Terminal-based plasmid map viewer, sequence editor, and cloning/mutagenesis workbench in pure Python

Batch primer design for genomic regions

Safe, idiomatic Rust bindings to the primer3 C library for PCR primer design and oligonucleotide thermodynamic calculations

A high-performance Rust implementation of primer3 design, leveraging PyO3 to interface with Python's primer3 package. This tool provides fast and reliable primer design for qPCR applications with thermodynamic filtering.

Local, open-source Primer-BLAST for plant breeding: Primer3 + BLAST+, multi-reference specificity, in-silico PCR, CAPS/dCAPS, offline

Focused WSL-backed exon primer and CAPS marker GUI for Windows

Species-specific primer design tool: NCBI retrieval → BLAST mismatch analysis → Primer3 design → Primer-BLAST validation. Desktop GUI (PySide6) + PDF/DOCX reports.

Focused WSL-backed Primer3 and primer specificity GUI for Windows

WebAssembly build of primer3 for client-side PCR primer and probe design

A collection of simple python tools for molecular biology and bioinformatics

CE-specific qPCR primer design pipeline: Primer3 + pair-wise BLAST against genome & curated RefSeq mRNA to select junction-spanning assays.

Simple shiny app to analyze primer quality.

A web app interface for Primer3 using Streamlit and primer3-py, modeled after Primer3Plus