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Bioinformatics analysis of the monkey pox virus

Workshop - Accra, Ghana, July 2026

This repository contains the materials and documentation for the workshop on the bioinformatics of the monkey pox virus

Instructors

  • Marie Lataretu
  • Torsten Houwaart

Schedule

Linux introduction, terminal basics, navigation, file management, viewing files, editing text.

Time Activity
09:00 - 12:00 Check-in & Laptop set up
12:00 - 13:00 🍽️ Lunch Break
13:00 - 16:30 Linux CLI
Time Activity
09:00 - 09:45 Recap
09:45 - 12:00 Trimmomatic (with coffee break in between)
12:00 - 13:00 🍽️ Lunch Break
13:00 - 16:30 BWA mem and Freebayes (with coffee break in between)
Time Activity
09:00 - 09:30 Recap
09:30 - 09:45 Pipelines and workflow manager
09:45 - 10:30 Genome reconstruction with amplicon-nf
10:30 - 10:45 ☕ Break
10:45 - 12:00 Downstream analysis I: Nextclade
12:00 - 13:00 🍽️ Lunch Break
13:00 - 14:30 Downstream analysis II: Phylogenetic trees
14:30 - 15:30 Data analysis
15:30 - 16:00 Recap & Closing remarks

Recommended setup

Clone the repository into your home directory:

cd ~
git clone https://github.com/rki-mf1/2026-Workshop-Ghana.git
cd ~/2026-Workshop-Ghana

How to use this repository

  • Start at the README.md of the relevant day.
  • Follow the exercises in numerical order.
  • Use a scratch or practice directory when a lesson asks you to create, move, rename, or delete files.

About

Introduction to the linux command line and the bioinformatics of ONT-based sequencing of the monkey pox virus

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