Releases: hisplan/sharp
Releases · hisplan/sharp
v0.1.1
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Fixed the problem where one vs. rest plots fail if negative exists.
Other minor updates.
v0.1.0
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Adds automated basic QC notebook for Cell Hashing (aka. hashtag) and CITE-SEQ.
Disables read_from_cache, enables write_to_cache by default.
v0.0.13
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Added missing submit-cellplex.sh.
Added absolute minimum tutorial notebook.
v0.0.12
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Added proper handle for antibody name composed of just numbers.
Added outputs overview document.
Updated QC notebooks
Updated job templates
Removed deprecated code
v0.0.11
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v0.0.10
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Handles an edge case where doublets don't exist.
Refactored for SCING.
Uses seqc-utils:0.5.1.
v0.0.9
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Supports ASAP-seq.
Generates AnnData for all assays.
Updates the inspection scripts to use the generated AnnData.
Changes the workflow name from Sharp to Hashtag
Uses hto_gex_mapper to speed up the HTO <--> GEX translation process.
Adds maxRetries: 3 to the workflow option file.
Removed unnecessary input variables from CiteSeq and Preprocess.
Adds unit tests
v0.0.8
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Refactored to support Amazon ECR.
Renamed QC to SanityCheck.
Removed sc.pp.calculate_qc_metrics from CITE-seq AnnData.
v0.0.7
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Added demuxMode for K-Means based demultiplexing: 1=default, 2=noisy methanol, 3=aggressively rescue from doublets
Added minCount: total count for CB less than this threshold will be marked as negative (unreliable observations)
Beta support for 10x Cell Multiplexing (aka. cellplex)
v0.0.6
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Auto-computes memory requirements if -1 is passed to Sharp.resourceSpec. This helps process 400M+ reads and/or 20K+ cells
Refactored to support different versions of CITE-seq-Count.
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