Reanalysis of the optical pooled CRISPR screen from Funk et al. 2022 (Cell) using brieflow v1.4.10.
| Parameter | Value |
|---|---|
| Cell line | HeLa-TetR-Cas9 (A7) |
| Genes targeted | 5,299 |
| Total guides | 20,445 (4 per gene) |
| Plates | 8 |
| Phenotype markers | DAPI, Tubulin (alpha-tubulin-FITC), γ-H2AX (AF594), Phalloidin (AF750) |
Raw data was processed end-to-end with brieflow, covering preprocessing, SBS decoding, phenotype feature extraction, merge, aggregate, and clustering steps. Configuration notebooks and Slurm scripts are in analysis/.
Raw imaging data (ND2 format) and brieflow outputs are stored at /lab/ops_analysis_ssd/cheeseman/aconcagua-analysis/. Source data used for figure generation is in analysis/source_data/.
Citation will be added upon publication.