Local-first bioinformatics tools for DTC DNA conversion, VCF/gVCF processing, genetic variant annotation, and research-grade desktop and CLI workflows.
biotec-line builds practical Python desktop software for working with sensitive genetic files on the user's own machine. The current projects focus on converting direct-to-consumer DNA exports into VCF 4.2, validating genome builds, annotating VCF/gVCF data, filtering variants, and exporting research-oriented results without turning genetic data into a cloud workflow.
| Need | Start with | Why |
|---|---|---|
| Convert 23andMe, MyHeritage, FTDNA, or similar raw DNA exports to VCF 4.2 | genotype-to-vcf | PySide6 desktop converter and headless CLI with GRCh37/GRCh38 detection, dbSNP/FASTA reference lookup, SHA256 release verification, local cache, and privacy-focused VCF output. Runs on Windows, macOS, and Linux. |
| Annotate, filter, inspect, and export VCF/gVCF or 23andMe-derived variant files | VFDistiller | Local-first variant analysis GUI with gnomAD, MyVariant.info, VEP, ALFA, TOPMed, ClinVar-oriented fields, FASTA validation, CSV/Excel/PDF/VCF export, dependency audit, and cross-platform CI smoke tests |
This directory lists the public repositories that are part of the biotec-line organization profile. Private, internal, or unreleased research work is intentionally not listed here.
| Repository | Role | Public status |
|---|---|---|
| .github | Organization profile, shared issue templates, contribution policy, security policy, and machine-readable llms.txt context |
Active |
| genotype-to-vcf | DTC DNA raw-data to VCF 4.2 converter for 23andMe, MyHeritage, FTDNA, GRCh37/GRCh38, dbSNP, FASTA, SHA256 release checksums, and CLI pipeline workflows | Active |
| VFDistiller | Local-first VCF/gVCF annotation, filtering, validation, dependency audit, and export desktop tool for research-use-only variant workflows | Active |
| Project | Description |
|---|---|
| genotype-to-vcf | Converts four-column DTC DNA raw data into VCF 4.2 with automatic build detection, sex-aware ploidy handling, dbSNP integration, optional offline FASTA lookup, headless CLI mode for macOS/Linux pipelines, SHA256SUMS release verification, and local-only file handling |
| Project | Description |
|---|---|
| VFDistiller | Local-first desktop app for VCF, gVCF, 23andMe raw text, and FASTA workflows, including annotation, filtering, allele-frequency lookup, reference validation, research exports, and cross-platform source smoke tests (Windows, macOS, Linux) |
These tools are built for bioinformatics research, teaching, software development, and reproducible local workflows. They are not medical devices, not in-vitro diagnostic products, not clinically validated, and not intended for diagnosis, prognosis, therapy decisions, or clinical interpretation of genetic results.
Users remain responsible for lawful handling of genetic data, informed consent, data minimization, local storage security, and interpretation by qualified professionals where health-related questions are involved.
- Local first: raw genetic files, generated VCFs, caches, reference genomes, and settings stay on the user's machine by default.
- Privacy-conscious: tools avoid transmitting genotype data or raw files; optional external calls are limited and documented, such as rsID lookup or reference genome downloads.
- Research-use clarity: README files and application surfaces keep the non-clinical, non-diagnostic boundary visible.
- Cross-platform: desktop GUI on Windows; headless CLI for macOS and Linux pipeline integration.
- Inspectable data flow: conversion, annotation, filtering, caches, ignored local artifacts, and export formats are documented for maintainers and LLM-assisted review.
For crawlers and LLM tools, see llms.txt. It lists canonical repositories, project roles, research-use boundaries, and preferred search phrases for the biotec-line organization.
Useful GitHub and web search phrases include biotec-line VCF tools, DTC DNA to VCF converter, 23andMe MyHeritage VCF conversion, local-first VCF annotation desktop app, genotype-to-vcf CLI headless pipeline, gVCF filtering GUI, and research-use-only genetic variant annotation software.
biotec-line is the bioinformatics branch of the broader research and AI tool ecosystem: