AttributeError: 'NoneType' object has no attribute 'strip' #6

I am at the first step and used the following command:
python run_preProcess_by_chr_step1.py -i /media/ambreen/D206B4E906B4CFA9/Mouse/TG_TS_RNA/alignment/123 -o /media/ambreen/D206B4E906B4CFA9/Mouse/TG_TS_RNA/alignment/ --nice
But it is giving me following error:
Traceback (most recent call last):
File "run_preProcess_by_chr_step1.py", line 227, in
if name == "main": main()
File "run_preProcess_by_chr_step1.py", line 178, in main
cmd += "%s " % preProcess_options.strip()
AttributeError: 'NoneType' object has no attribute 'strip'

the txt file i am loading is attached.
123.txt

what is wrong with file?