HBB_MiniProject_ShreyaBansode

📌 Project Title

Evolutionary Analysis of the HBB Gene Across Multiple Species

🧬 Objective

To explore the evolutionary conservation of the HBB (Hemoglobin subunit beta) gene across six species using pairwise alignment, multiple sequence alignment, sequence logo analysis, and phylogenetic tree construction.

🔧 Tools Used

• NCBI Protein Database – Sequence retrieval
• EMBOSS Needle – Pairwise alignment
• Clustal Omega – Multiple Sequence Alignment
• Skylign – Sequence logo generation
• MEGA X – Phylogenetic tree construction
• MS Word / Google Docs – Report preparation

🧪 Methodology

1. Retrieved HBB protein sequences from NCBI for six species.
2. Conducted pairwise alignment using EMBOSS Needle.
3. Performed multiple sequence alignment using Clustal Omega.
4. Generated a sequence logo to visualize conserved residues.
5. Constructed a phylogenetic tree using MEGA X.
6. Documented findings in a comprehensive report.

📄 Report

The complete report is available in the repository as:
HBB_MiniProject_ShreyaBansode.pdf

📷 Screenshots

All key results and tool outputs (alignment, logo, tree) are included in the report.

✅ Submission

Submitted the GitHub link via the official Google Form as per the project guidelines.

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📁 Repository Structure