CasanovoGUI is a cross-platform desktop application for Casanovo — de novo peptide sequencing from MS/MS spectra with a transformer model. Configure your inputs and parameters in a simple form, click Run Casanovo, and watch the output stream live in the console. The GUI wraps Casanovo's command-line sub-commands — de novo sequencing, database search, evaluation, and training — each as its own tab, plus a View tab to explore your results: map peptides to a reference proteome, inspect score distributions and per-residue confidence, and open spectra in PDV for annotated-spectrum visualization.
Download the installer for your platform from the Releases page:
| OS | Download | How to run |
|---|---|---|
| Windows | CasanovoGUI-<version>-windows-x64.zip |
Unzip and run CasanovoGUI.exe |
| macOS (Apple Silicon) | CasanovoGUI-<version>-macos-arm64.dmg |
Open and drag to Applications |
| Linux | CasanovoGUI-<version>-linux-x86_64.deb or …-linux-x86_64.tar.gz |
Install the .deb, or just extract the .tar.gz (no root) and run |
No manual tool installation is needed. The installers bundle their own Java
runtime, so you do not have to install Java. And the first time you start an
analysis, if Casanovo isn't already on your machine the GUI offers to install it
for you — it downloads a private Python and Casanovo automatically (no Python or
pip setup required). Just download, launch, and run.
Intel Macs: use the cross-platform
CasanovoGUI-<version>.jarfrom the Releases page instead (it needs a Java 23+ runtime installed).
For a complete guide to using CasanovoGUI — installing it, running each task (de novo sequencing, database search, evaluation, and training), and exploring your results in the View tab — see Getting Started: Graphical User Interface in the Casanovo documentation.