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RUN_GISTIC:ASSEMBLE_GISTIC_OUTPUT - KeyError: 'Unique Name' #33
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Description of the bug
Hello, good afternoon !
I encountered the following error while trying to run GISTIC along with ichorCNA. The error message refers to a missing column named 'Unique Name', but I am unsure in which file this column is supposed to be present.
This issue appeared after I made the latest pull to version v1.0.6. Before this update, GISTIC was running without any problems. I also had the same issue with qpdf, as described in #32.
I am attaching the .nextflow.log, command.sh, and .command.run files for reference.
Thank you in advance for your help, and congratulations on your latest publication of SAMURAI! :D
executor > local (2)
[71/96fd69] process > DINCALCILAB_SAMURAI:SAMURAI:SAMTOOLS_INDEX (16_11418_R) [100%] 6 of 6, cached: 6 ✔
[b3/ec30fc] process > DINCALCILAB_SAMURAI:SAMURAI:BAM_QC_PICARD:PICARD_COLLECTMULTIPLEMETRIC... [100%] 6 of 6, cached: 6 ✔
[- ] process > DINCALCILAB_SAMURAI:SAMURAI:BAM_QC_PICARD:PICARD_COLLECTHSMETRICS -
[e7/5de44c] process > DINCALCILAB_SAMURAI:SAMURAI:BAM_QC_PICARD:PICARD_COLLECTWGSMETRICS (VH... [100%] 6 of 6, cached: 6 ✔
[67/736e2a] process > DINCALCILAB_SAMURAI:SAMURAI:LIQUID_BIOPSY:HMMCOPY_READCOUNTER_ICHORCNA... [100%] 6 of 6, cached: 6 ✔
[5f/f979ef] process > DINCALCILAB_SAMURAI:SAMURAI:LIQUID_BIOPSY:RUN_ICHORCNA (M16_1190_R) [100%] 6 of 6, cached: 6 ✔
[c4/ff4d01] process > DINCALCILAB_SAMURAI:SAMURAI:LIQUID_BIOPSY:AGGREGATE_ICHORCNA_TABLE (As... [100%] 1 of 1, cached: 1 ✔
[93/45a501] process > DINCALCILAB_SAMURAI:SAMURAI:LIQUID_BIOPSY:CORRECT_LOGR_ICHORCNA (Corre... [100%] 1 of 1, cached: 1 ✔
[62/bb08b1] process > DINCALCILAB_SAMURAI:SAMURAI:LIQUID_BIOPSY:CONCATENATE_BIN_PLOTS (Conca... [100%] 1 of 1, cached: 1 ✔
[d5/6721e1] process > DINCALCILAB_SAMURAI:SAMURAI:RUN_GISTIC:GISTIC2 (Run Gistic2) [100%] 1 of 1, cached: 1 ✔
[52/316646] process > DINCALCILAB_SAMURAI:SAMURAI:RUN_GISTIC:ASSEMBLE_GISTIC_OUTPUT (Rearran... [ 0%] 0 of 1
[fc/fcc57e] process > DINCALCILAB_SAMURAI:SAMURAI:RUN_GISTIC:MAFTOOLS (Maftools Plot) [ 0%] 0 of 1
[- ] process > DINCALCILAB_SAMURAI:SAMURAI:MULTIQC
-[dincalcilab/samurai] Pipeline completed with errors-
ERROR ~ Error executing process > 'DINCALCILAB_SAMURAI:SAMURAI:RUN_GISTIC:ASSEMBLE_GISTIC_OUTPUT (Rearrange GISTIC2 output)'
Caused by:
Process `DINCALCILAB_SAMURAI:SAMURAI:RUN_GISTIC:ASSEMBLE_GISTIC_OUTPUT (Rearrange GISTIC2 output)` terminated with an error exit status (1)
Command executed:
gistic lesion_table -t all --genome hg38 gistic_results gistic_summary_mqc
mv gistic_summary_mqc.focal_lesions.txt gistic_summary_mqc.txt
# Only do the rename if the file was produced, i.e. when broad analysis runs
if [ -f gistic_summary_mqc.broad_lesions.txt ]; then
mv gistic_summary_mqc.broad_lesions.txt gistic_broad_lesions_mqc.txt
fi
gistic gene_table --genome hg38 gistic_results gistic
gistic copy_number_state_table --genome hg38 gistic_results gistic_cn_states.txt
gistic log2ratio_table --genome hg38 gistic_results gistic_log2R.txt
cat <<-END_VERSIONS > versions.yml
"DINCALCILAB_SAMURAI:SAMURAI:RUN_GISTIC:ASSEMBLE_GISTIC_OUTPUT":
gistic-cli: $(gistic --version | cut -d ' ' -f2)
END_VERSIONS
Command used and terminal output
nextflow run dincalcilab/samurai --input short_ver_test_new_version_samurai.csv -profile docker --outdir SAMURAI_SHORT_VER_RUN_FROM_BAM --genome hg38 --fasta ~/Reference/bwa_GRCh38/Homo_sapiens.GRCh38.dna.primary_assembly.fa --fai ~/Reference/bwa_GRCh38/Homo_sapiens.GRCh38.dna.primary_assembly.fa.fai --dict ~/Reference/bwa_GRCh38/Homo_sapiens.GRCh38.dna.primary_assembly.fa.dict --analysis_type liquid_biopsy --caller ichorcna --binsize 1000 --normal_panel ~/Projects/ichorcna_u/ichorCNA_U/src_ichorCNA/inst/extdata/HD_ULP_PoN_1Mb_median_normAutosome_mapScoreFiltered_median.rds --ichorcna_readcounter_chrs "1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,X,Y" --ichorcna_chrs_to_use "c(1:22)" --ichorcna_chrs_to_train "c(1:22)" --ichorcna_chrs_to_normalize "c(1:22)" --ichorcna_estimate_sc TRUE --ichorcna_gc_wig ~/Projects/ichorcna_u/ichorCNA_U/src_ichorCNA/inst/extdata/gc_hg38_1000kb.wig --ichorcna_map_wig ~/Projects/ichorcna_u/ichorCNA_U/src_ichorCNA/inst/extdata/map_hg38_1000kb.wig --ichorcna_centromere_file ~/Projects/ichorcna_u/ichorCNA_U/src_ichorCNA/inst/extdata/GRCh38.GCA_000001405.2_centromere_acen.txt --ichorcna_normal_states "c(0.5,0.6,0.7,0.8,0.9)" --ichorcna_plotylim "c(-2, 2)" --max_memory 28.GB --compute_signatures TRUE --run_gistic TRUE --gistic_broad_analysis TRUE -resumeRelevant files
System information
N E X T F L O W ~ version 23.10.1
Container: Docker
OS: Linux
Version: dincalcilab/samurai v1.0.6-gb82e24f
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lbeltrame commentedon Feb 4, 2025
Can you check the header output files from GISTIC directly (in particular
all_lesions_conf_xx.txtwhere xx is your confidence level)?Unique Nameshould be a column in the GISTIC output: it may mean that no regions where identified.SaraPotente commentedon Jul 2, 2026
No response. Closed.