MAnorm2_utils could not process the read file

[maisarahabs]

Hi.
I converted my bam files to sam. Then I used sam2bed.

I used the command profile bins for my peak files and read files; both were in bed format.

The tool could read the peak files but could not process the read files.

============

Produced by profile_bins, version=1.0.0

Equivalent parameter settings:
peaks=suz12_13CF.bed,suz12_14CF.bed
reads=suz12_13.markdup.bed,suz12_14.markdup.bed
labs=s13,s14
n=suz12
min-peak-gap=150
typical-bin-size=1500
summits=using_middle_points
paired=True
keep-dup=all
method=byBin
fix-bin-size=False

Summary of peak numbers in peak files:
peak_file peaks_after_filtering peaks_after_merging
suz12_13CF.bed 36694 36694
suz12_14CF.bed 38237 38237

Divide merged peaks into reference genomic bins:
77196 bins in total

Errors occur when parsing the read file,
suz12_14.markdup.bed:

Program aborted.

Please advice. Thank you.

[tushiqi]

@maisarahabs
Sincerely sorry for my late response.
Typically it is because of the format of the read file. But it is indeed strange that the program has not printed any information about the specific problem. Could you please send me the read file so that I can track the progress of the program?

Thanks.