Hi,
Thanks for developing MAnorm.
I have a question regarding normalisation.
Is it possible to normalise by spike-in or using in such way that doesn't correct the library size in each condition?
The way I normalised was by inserting 10% of spike-in mice control with my human cells samples.
I mapped each to its genome, creating a factor between the number of mapped mice reads and human ones and correcting the library size of each sample by the factor. In such a way, I can assume that the quantitative difference I'm observing is biological..
If I understood right, MAnorm ignores that. So it would be great if I could skip any lib size correction.
Hope to hear from you.
many thanks
Hi,
Thanks for developing MAnorm.
I have a question regarding normalisation.
Is it possible to normalise by spike-in or using in such way that doesn't correct the library size in each condition?
The way I normalised was by inserting 10% of spike-in mice control with my human cells samples.
I mapped each to its genome, creating a factor between the number of mapped mice reads and human ones and correcting the library size of each sample by the factor. In such a way, I can assume that the quantitative difference I'm observing is biological..
If I understood right, MAnorm ignores that. So it would be great if I could skip any lib size correction.
Hope to hear from you.
many thanks