In my .fcs file, spillover data available in $SPILLOVER and other $spill and $spillover have null; how Do I perform compensation? I tried, but it gave me an error
fs_comp<- compensate(fs, spillover(fs[[1]])$SPILLOVER)
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function ‘compensate’ for signature ‘"flowFrame", "NULL"’
In my .fcs file, spillover data available in $SPILLOVER and other $spill and $spillover have null; how Do I perform compensation? I tried, but it gave me an error
fs_comp<- compensate(fs, spillover(fs[[1]])$SPILLOVER)
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function ‘compensate’ for signature ‘"flowFrame", "NULL"’