From 7deb24270bd31f16043669a00c5d380603ccad6a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Apr 2024 10:40:55 +0200
Subject: [PATCH 001/887] bump version to 4.0.0dev

---
 CHANGELOG.md              | 10 ++++++++++
 assets/multiqc_config.yml |  4 ++--
 nextflow.config           |  2 +-
 3 files changed, 13 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 871c46594..18fabee33 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,16 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## v4.0.0dev - [date]
+
+### Added
+
+### Changed
+
+### Fixed
+
+### Removed
+
 ## v3.0.2 - [2024-04-10]
 
 ### Added
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index fe0459889..963555cf6 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,7 +1,7 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/rnafusion/releases/tag/3.0.2" target="_blank">nf-core/rnafusion</a>
+  This report has been generated by the <a href="https://github.com/nf-core/rnafusion/tree/dev" target="_blank">nf-core/rnafusion</a>
   analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/rnafusion/3.0.2/docs/output" target="_blank">documentation</a>.
+  <a href="https://nf-co.re/rnafusion/dev/docs/output" target="_blank">documentation</a>.
 
 report_section_order:
   nf-core-rnafusion-methods-description:
diff --git a/nextflow.config b/nextflow.config
index 17375158e..22799afed 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -302,7 +302,7 @@ manifest {
     description     = """Nextflow rnafusion analysis pipeline, part of the nf-core community."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '3.0.2'
+    version         = '4.0.0dev'
     doi             = ''
 }
 

From 8f794b39585dec444eb2a0e1617ad7938e355026 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Apr 2024 12:42:45 +0200
Subject: [PATCH 002/887] get arriba from nf-core

---
 conf/modules.config                           |   2 +-
 modules.json                                  |  46 +-
 modules/nf-core/arriba/arriba/environment.yml |   7 +
 modules/nf-core/arriba/arriba/main.nf         |  66 +++
 modules/nf-core/arriba/arriba/meta.yml        | 112 ++++
 .../nf-core/arriba/download/environment.yml   |   7 +
 .../arriba/download/main.nf                   |  17 +-
 .../arriba/download/meta.yml                  |   3 +-
 modules/nf-core/cat/cat/main.nf               |  23 +-
 modules/nf-core/cat/cat/tests/main.nf.test    |  45 +-
 modules/nf-core/cat/fastq/environment.yml     |   2 +-
 modules/nf-core/cat/fastq/main.nf             |   1 -
 modules/nf-core/cat/fastq/tests/main.nf.test  |  63 ++-
 .../nf-core/cat/fastq/tests/main.nf.test.snap | 185 +++++--
 modules/nf-core/fastp/main.nf                 |  26 +-
 modules/nf-core/fastp/tests/main.nf.test      | 332 ++++++++++--
 modules/nf-core/fastp/tests/main.nf.test.snap | 290 ++++++++++-
 .../fastp/tests/nextflow.interleaved.config   |   5 +
 ...low.config => nextflow.save_failed.config} |   3 +-
 modules/nf-core/fastqc/main.nf                |   6 +
 .../gatk4/bedtointervallist/environment.yml   |   2 +-
 .../nf-core/gatk4/bedtointervallist/main.nf   |   4 +-
 .../bedtointervallist/tests/main.nf.test      |  38 ++
 .../bedtointervallist/tests/main.nf.test.snap |  35 ++
 .../gatk4/bedtointervallist/tests/tags.yml    |   2 +
 .../createsequencedictionary/environment.yml  |   2 +-
 .../gatk4/createsequencedictionary/main.nf    |   4 +-
 .../gatk4/markduplicates/environment.yml      |   5 +-
 modules/nf-core/gatk4/markduplicates/main.nf  |   4 +-
 .../gatk4/markduplicates/tests/bam.config     |   8 +
 .../gatk4/markduplicates/tests/cram.config    |   8 +
 .../gatk4/markduplicates/tests/main.nf.test   | 126 +++++
 .../markduplicates/tests/main.nf.test.snap    | 160 ++++++
 .../gatk4/markduplicates/tests/tags.yml       |   2 +
 .../collectinsertsizemetrics/environment.yml  |   2 +-
 .../picard/collectinsertsizemetrics/main.nf   |   4 +-
 .../picard/collectinsertsizemetrics/meta.yml  |   2 +-
 .../picard/collectwgsmetrics/environment.yml  |   2 +-
 .../nf-core/picard/collectwgsmetrics/main.nf  |   4 +-
 .../nf-core/samtools/faidx/environment.yml    |   5 +-
 modules/nf-core/samtools/faidx/main.nf        |   4 +-
 modules/nf-core/samtools/faidx/meta.yml       |   4 +
 .../nf-core/samtools/faidx/tests/main.nf.test | 122 +++++
 .../samtools/faidx/tests/main.nf.test.snap    | 249 +++++++++
 .../samtools/faidx/tests/nextflow.config      |   7 +
 .../samtools/faidx/tests/nextflow2.config     |   6 +
 modules/nf-core/samtools/faidx/tests/tags.yml |   2 +
 .../nf-core/samtools/index/environment.yml    |   3 +-
 modules/nf-core/samtools/index/main.nf        |   4 +-
 .../nf-core/samtools/index/tests/main.nf.test |  36 +-
 .../samtools/index/tests/main.nf.test.snap    |  54 +-
 modules/nf-core/samtools/sort/environment.yml |   3 +-
 modules/nf-core/samtools/sort/main.nf         |  32 +-
 modules/nf-core/samtools/sort/meta.yml        |  32 +-
 .../nf-core/samtools/sort/tests/main.nf.test  |  68 ++-
 .../samtools/sort/tests/main.nf.test.snap     | 127 ++++-
 .../samtools/sort/tests/nextflow.config       |   3 +-
 modules/nf-core/samtools/view/environment.yml |   3 +-
 modules/nf-core/samtools/view/main.nf         |  17 +-
 .../nf-core/samtools/view/tests/bam.config    |   3 +
 .../samtools/view/tests/bam_index.config      |   3 +
 .../nf-core/samtools/view/tests/main.nf.test  | 212 ++++++++
 .../samtools/view/tests/main.nf.test.snap     | 488 ++++++++++++++++++
 modules/nf-core/samtools/view/tests/tags.yml  |   2 +
 modules/nf-core/star/align/environment.yml    |   3 +-
 modules/nf-core/star/align/main.nf            |   4 +-
 modules/nf-core/star/align/tests/main.nf.test | 141 ++---
 .../star/align/tests/main.nf.test.snap        |  68 +--
 .../star/genomegenerate/environment.yml       |   3 +-
 modules/nf-core/star/genomegenerate/main.nf   |  87 ++--
 .../star/genomegenerate/tests/main.nf.test    |  89 +++-
 .../genomegenerate/tests/main.nf.test.snap    |  86 ++-
 .../stringtie/merge/tests/main.nf.test        |  48 +-
 .../stringtie/merge/tests/main.nf.test.snap   |  20 +-
 .../stringtie/stringtie/tests/main.nf.test    |  64 ++-
 .../stringtie/stringtie/tests/nextflow.config |   5 +
 subworkflows/local/arriba_workflow.nf         |  12 +-
 workflows/build_references.nf                 |   2 +-
 78 files changed, 3244 insertions(+), 532 deletions(-)
 create mode 100644 modules/nf-core/arriba/arriba/environment.yml
 create mode 100644 modules/nf-core/arriba/arriba/main.nf
 create mode 100644 modules/nf-core/arriba/arriba/meta.yml
 create mode 100644 modules/nf-core/arriba/download/environment.yml
 rename modules/{local => nf-core}/arriba/download/main.nf (65%)
 rename modules/{local => nf-core}/arriba/download/meta.yml (95%)
 create mode 100644 modules/nf-core/fastp/tests/nextflow.interleaved.config
 rename modules/nf-core/fastp/tests/{nextflow.config => nextflow.save_failed.config} (50%)
 create mode 100644 modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test
 create mode 100644 modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/gatk4/bedtointervallist/tests/tags.yml
 create mode 100644 modules/nf-core/gatk4/markduplicates/tests/bam.config
 create mode 100644 modules/nf-core/gatk4/markduplicates/tests/cram.config
 create mode 100644 modules/nf-core/gatk4/markduplicates/tests/main.nf.test
 create mode 100644 modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/gatk4/markduplicates/tests/tags.yml
 create mode 100644 modules/nf-core/samtools/faidx/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/faidx/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/faidx/tests/nextflow.config
 create mode 100644 modules/nf-core/samtools/faidx/tests/nextflow2.config
 create mode 100644 modules/nf-core/samtools/faidx/tests/tags.yml
 create mode 100644 modules/nf-core/samtools/view/tests/bam.config
 create mode 100644 modules/nf-core/samtools/view/tests/bam_index.config
 create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/view/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/view/tests/tags.yml
 create mode 100644 modules/nf-core/stringtie/stringtie/tests/nextflow.config

diff --git a/conf/modules.config b/conf/modules.config
index 9d4ca62b1..dfb660787 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -18,7 +18,7 @@ process {
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
-    withName: 'ARRIBA' {
+    withName: 'ARRIBA_ARRIBA' {
         publishDir = [
             path: { "${params.outdir}/arriba" },
             mode: params.publish_dir_mode,
diff --git a/modules.json b/modules.json
index 23a6ce29f..f14cf897a 100644
--- a/modules.json
+++ b/modules.json
@@ -12,42 +12,52 @@
                     },
                     "arriba": {
                         "branch": "master",
-                        "git_sha": "ea9e2892a9d12e8769402f12096219942bcf6536",
+                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
-                    "cat/cat": {
+                    "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
-                    "cat/fastq": {
+                    "arriba/download": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
+                    "cat/cat": {
+                        "branch": "master",
+                        "git_sha": "9437e6053dccf4aafa022bfd6e7e9de67e625af8",
+                        "installed_by": ["modules"]
+                    },
+                    "cat/fastq": {
+                        "branch": "master",
+                        "git_sha": "0997b47c93c06b49aa7b3fefda87e728312cf2ca",
+                        "installed_by": ["modules"]
+                    },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
+                        "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
                         "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "d3f215802f696f7993f25c759781d2db91232015",
                         "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980",
                         "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "194fca815cf594646e638fa5476acbcc296f1850",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
@@ -57,52 +67,52 @@
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
                         "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
                         "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "f153f1f10e1083c49935565844cccb7453021682",
                         "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8",
+                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
                         "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "4352dbdb09ec40db71e9b172b97a01dcf5622c26",
                         "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "0bd7d2333a88483aa0476acea172e9f5f6dd83bb",
                         "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "9f6b233518f7d9ecdcf24b798b7e491db5424273",
+                        "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9",
                         "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
-                        "git_sha": "0e98289b5bec6e3f8f588a8a9d05e8aacc1179a0",
+                        "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9",
                         "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
-                        "git_sha": "b0dcb44b018d9b2bcb35b1abb7bcd34061bc5a6d",
+                        "git_sha": "5756b12b1fab0c07f05b9ad2d5d5c262b1a2c1ef",
                         "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "b0dcb44b018d9b2bcb35b1abb7bcd34061bc5a6d",
+                        "git_sha": "b1b959609bda44341120aed1766329909f54b8d0",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/arriba/arriba/environment.yml b/modules/nf-core/arriba/arriba/environment.yml
new file mode 100644
index 000000000..8494c8c03
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/environment.yml
@@ -0,0 +1,7 @@
+name: arriba_arriba
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::arriba=2.4.0
diff --git a/modules/nf-core/arriba/arriba/main.nf b/modules/nf-core/arriba/arriba/main.nf
new file mode 100644
index 000000000..a29a8d140
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/main.nf
@@ -0,0 +1,66 @@
+process ARRIBA_ARRIBA {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
+        'biocontainers/arriba:2.4.0--h0033a41_2' }"
+
+    input:
+    tuple val(meta), path(bam)
+    tuple val(meta2), path(fasta)
+    tuple val(meta3), path(gtf)
+    tuple val(meta4), path(blacklist)
+    tuple val(meta5), path(known_fusions)
+    tuple val(meta6), path(structural_variants)
+    tuple val(meta7), path(tags)
+    tuple val(meta8), path(protein_domains)
+
+    output:
+    tuple val(meta), path("*.fusions.tsv")          , emit: fusions
+    tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
+    path "versions.yml"                             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
+    def known_fusions = known_fusions ? "-k $known_fusions" : ""
+    def structural_variants = structural_variants ? "-d $structual_variants" : ""
+    def tags = tags ? "-t $tags" : ""
+    def protein_domains = protein_domains ? "-p $protein_domains" : ""
+
+    """
+    arriba \\
+        -x $bam \\
+        -a $fasta \\
+        -g $gtf \\
+        -o ${prefix}.fusions.tsv \\
+        -O ${prefix}.fusions.discarded.tsv \\
+        $blacklist \\
+        $known_fusions \\
+        $structural_variants \\
+        $tags \\
+        $protein_domains \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        arriba: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo stub > ${prefix}.fusions.tsv
+    echo stub > ${prefix}.fusions.discarded.tsv
+
+    echo "${task.process}:" > versions.yml
+    echo ' arriba: 2.2.1' >> versions.yml
+    """
+}
diff --git a/modules/nf-core/arriba/arriba/meta.yml b/modules/nf-core/arriba/arriba/meta.yml
new file mode 100644
index 000000000..11f744b54
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/meta.yml
@@ -0,0 +1,112 @@
+name: arriba_arriba
+description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
+keywords:
+  - fusion
+  - arriba
+  - detection
+  - RNA-Seq
+tools:
+  - arriba:
+      description: Fast and accurate gene fusion detection from RNA-Seq data
+      homepage: https://github.com/suhrig/arriba
+      documentation: https://arriba.readthedocs.io/en/latest/
+      tool_dev_url: https://github.com/suhrig/arriba
+      doi: "10.1101/gr.257246.119"
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - fasta:
+      type: file
+      description: Assembly FASTA file
+      pattern: "*.{fasta}"
+  - meta3:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - gtf:
+      type: file
+      description: Annotation GTF file
+      pattern: "*.{gtf}"
+  - meta4:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - blacklist:
+      type: file
+      description: Blacklist file
+      pattern: "*.{tsv}"
+  - meta5:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - known_fusions:
+      type: file
+      description: Known fusions file
+      pattern: "*.{tsv}"
+  - meta6:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - structural_variants:
+      type: file
+      description: Structural variants file
+      pattern: "*.{tsv}"
+  - meta7:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - tags:
+      type: file
+      description: Tags file
+      pattern: "*.{tsv}"
+  - meta8:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'test' ]
+  - protein_domains:
+      type: file
+      description: Protein domains file
+      pattern: "*.{gff3}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - fusions:
+      type: file
+      description: File contains fusions which pass all of Arriba's filters.
+      pattern: "*.{fusions.tsv}"
+  - fusions_fail:
+      type: file
+      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
+      pattern: "*.{fusions.discarded.tsv}"
+authors:
+  - "@praveenraj2018"
+  - "@rannick"
+maintainers:
+  - "@praveenraj2018"
+  - "@rannick"
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
new file mode 100644
index 000000000..dc7d3bf99
--- /dev/null
+++ b/modules/nf-core/arriba/download/environment.yml
@@ -0,0 +1,7 @@
+name: arriba_download
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::arriba=2.4.0
diff --git a/modules/local/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
similarity index 65%
rename from modules/local/arriba/download/main.nf
rename to modules/nf-core/arriba/download/main.nf
index 860439ade..7f11898bd 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -1,15 +1,18 @@
 process ARRIBA_DOWNLOAD {
     tag "arriba"
-    label 'process_low'
+    label 'process_single'
 
-    conda "bioconda::gnu-wget=1.18"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
-        'quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
+        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
+        'biocontainers/arriba:2.4.0--h0033a41_2' }"
 
     output:
-    path "versions.yml"   , emit: versions
     path "*"              , emit: reference
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
 
     script:
     """
@@ -21,7 +24,7 @@ process ARRIBA_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        arriba_download: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
     END_VERSIONS
     """
 
@@ -35,7 +38,7 @@ process ARRIBA_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        arriba_download: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
     END_VERSIONS
     """
 }
diff --git a/modules/local/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
similarity index 95%
rename from modules/local/arriba/download/meta.yml
rename to modules/nf-core/arriba/download/meta.yml
index 55e50b115..d32106163 100644
--- a/modules/local/arriba/download/meta.yml
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -3,6 +3,7 @@ description: Arriba is a command-line tool for the detection of gene fusions fro
 keywords:
   - fusion
   - arriba
+  - reference
 tools:
   - arriba:
       description: Fast and accurate gene fusion detection from RNA-Seq data
@@ -23,4 +24,4 @@ output:
       pattern: "*"
 
 authors:
-  - "@praveenraj2018, @rannick"
+  - "@peterpru"
diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf
index 4264a92cc..adbdbd7ba 100644
--- a/modules/nf-core/cat/cat/main.nf
+++ b/modules/nf-core/cat/cat/main.nf
@@ -22,6 +22,8 @@ process CAT_CAT {
     def args2 = task.ext.args2 ?: ''
     def file_list = files_in.collect { it.toString() }
 
+    // choose appropriate concatenation tool depending on input and output format
+
     // | input     | output     | command1 | command2 |
     // |-----------|------------|----------|----------|
     // | gzipped   | gzipped    | cat      |          |
@@ -30,11 +32,15 @@ process CAT_CAT {
     // | ungzipped | gzipped    | cat      | pigz     |
 
     // Use input file ending as default
-    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    prefix   = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}"
     out_zip  = prefix.endsWith('.gz')
     in_zip   = file_list[0].endsWith('.gz')
     command1 = (in_zip && !out_zip) ? 'zcat' : 'cat'
     command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : ''
+    if(file_list.contains(prefix.trim())) {
+        error "The name of the input file can't be the same as for the output prefix in the " +
+        "module CAT_CAT (currently `$prefix`). Please choose a different one."
+    }
     """
     $command1 \\
         $args \\
@@ -49,8 +55,12 @@ process CAT_CAT {
     """
 
     stub:
-    def file_list = files_in.collect { it.toString() }
-    prefix   = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    def file_list   = files_in.collect { it.toString() }
+    prefix          = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
+    if(file_list.contains(prefix.trim())) {
+        error "The name of the input file can't be the same as for the output prefix in the " +
+        "module CAT_CAT (currently `$prefix`). Please choose a different one."
+    }
     """
     touch $prefix
 
@@ -60,3 +70,10 @@ process CAT_CAT {
     END_VERSIONS
     """
 }
+
+// for .gz files also include the second to last extension if it is present. E.g., .fasta.gz
+def getFileSuffix(filename) {
+    def match = filename =~ /^.*?((\.\w{1,5})?(\.\w{1,5}\.gz$))/
+    return match ? match[0][1] : filename.substring(filename.lastIndexOf('.'))
+}
+
diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test
index 5766daafb..fcee2d19f 100644
--- a/modules/nf-core/cat/cat/tests/main.nf.test
+++ b/modules/nf-core/cat/cat/tests/main.nf.test
@@ -8,6 +8,32 @@ nextflow_process {
     tag "cat"
     tag "cat/cat"
 
+    test("test_cat_name_conflict") {
+        when {
+            params {
+                outdir   = "${outputDir}"
+            }
+            process {
+                """
+                input[0] =
+                    [
+                        [ id:'genome', single_end:true ],
+                        [
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true)
+                        ]
+                    ]
+                """
+            }
+        }
+        then {
+            assertAll(
+                { assert !process.success },
+                { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") }
+            )
+        }
+    }
+
     test("test_cat_unzipped_unzipped") {
         when {
             params {
@@ -19,8 +45,8 @@ nextflow_process {
                     [
                         [ id:'test', single_end:true ],
                         [
-                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true),
-                            file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true)
                         ]
                     ]
                 """
@@ -46,8 +72,8 @@ nextflow_process {
                     [
                         [ id:'test', single_end:true ],
                         [
-                            file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true),
-                            file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true)
                         ]
                     ]
                 """
@@ -76,8 +102,8 @@ nextflow_process {
                     [
                         [ id:'test', single_end:true ],
                         [
-                            file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true),
-                            file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true)
                         ]
                     ]
                 """
@@ -105,8 +131,8 @@ nextflow_process {
                     [
                         [ id:'test', single_end:true ],
                         [
-                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true),
-                            file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true)
                         ]
                     ]
                 """
@@ -134,7 +160,7 @@ nextflow_process {
                     [
                         [ id:'test', single_end:true ],
                         [
-                            file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
                         ]
                     ]
                 """
@@ -150,4 +176,3 @@ nextflow_process {
         }
     }
 }
-
diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml
index bff93add0..8c69b121f 100644
--- a/modules/nf-core/cat/fastq/environment.yml
+++ b/modules/nf-core/cat/fastq/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - conda-forge::sed=4.7
+  - conda-forge::coreutils=8.30
diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf
index 3d963784c..f132b2adc 100644
--- a/modules/nf-core/cat/fastq/main.nf
+++ b/modules/nf-core/cat/fastq/main.nf
@@ -76,5 +76,4 @@ process CAT_FASTQ {
             """
         }
     }
-
 }
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test b/modules/nf-core/cat/fastq/tests/main.nf.test
index f5f941825..dab2e14c0 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test
@@ -16,11 +16,11 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                            [ id:'test', single_end:true ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                            file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true) ]
-                        ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -28,8 +28,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -42,13 +41,13 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -56,8 +55,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -70,11 +68,11 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -82,8 +80,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -96,13 +93,13 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                    file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -110,8 +107,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
@@ -124,10 +120,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)]
+                ])
                 """
             }
         }
@@ -135,8 +131,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.reads).match() },
-                { assert path(process.out.versions.get(0)).getText().contains("cat") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test.snap b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
index ec2342e54..43dfe28fc 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test.snap
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
@@ -1,78 +1,169 @@
 {
     "test_cat_fastq_single_end": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:12.990284837"
+        "timestamp": "2024-01-17T17:30:39.816981"
     },
     "test_cat_fastq_single_end_same_name": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:31.554568147"
+        "timestamp": "2024-01-17T17:32:35.229332"
     },
     "test_cat_fastq_single_end_single_file": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.merged.fastq.gz:md5,e325ef7deb4023447a1f074e285761af"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:49.629360033"
+        "timestamp": "2024-01-17T17:34:00.058829"
     },
     "test_cat_fastq_paired_end_same_name": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
+            {
+                "0": [
                     [
-                        "test_1.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66",
-                        "test_2.merged.fastq.gz:md5,fe9f266f43a6fc3dcab690a18419a56e"
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
                     ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-17T23:19:40.711617539"
+        "timestamp": "2024-01-17T17:33:33.031555"
     },
     "test_cat_fastq_paired_end": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
                     [
-                        "test_1.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d",
-                        "test_2.merged.fastq.gz:md5,77c8e966e130d8c6b6ec9be52fcb2bda"
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
                     ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-10-18T07:53:20.923560211"
+        "timestamp": "2024-01-17T17:32:02.270935"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf
index c8e815aea..4fc19b744 100644
--- a/modules/nf-core/fastp/main.nf
+++ b/modules/nf-core/fastp/main.nf
@@ -29,7 +29,7 @@ process FASTP {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : ""
-    def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
+    def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--failed_out ${prefix}.paired.fail.fastq.gz --unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
     // Added soft-links to original fastqs for consistent naming in MultiQC
     // Use single ended for interleaved. Add --interleaved_in in config.
     if ( task.ext.args?.contains('--interleaved_in') ) {
@@ -45,7 +45,7 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> ${prefix}.fastp.log \\
+            2> >(tee ${prefix}.fastp.log >&2) \\
         | gzip -c > ${prefix}.fastp.fastq.gz
 
         cat <<-END_VERSIONS > versions.yml
@@ -66,7 +66,7 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> ${prefix}.fastp.log
+            2> >(tee ${prefix}.fastp.log >&2)
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
@@ -91,7 +91,7 @@ process FASTP {
             --thread $task.cpus \\
             --detect_adapter_for_pe \\
             $args \\
-            2> ${prefix}.fastp.log
+            2> >(tee ${prefix}.fastp.log >&2)
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
@@ -99,4 +99,22 @@ process FASTP {
         END_VERSIONS
         """
     }
+
+    stub:
+    def prefix              = task.ext.prefix ?: "${meta.id}"
+    def is_single_output    = task.ext.args?.contains('--interleaved_in') || meta.single_end
+    def touch_reads         = is_single_output ? "${prefix}.fastp.fastq.gz" : "${prefix}_1.fastp.fastq.gz ${prefix}_2.fastp.fastq.gz"
+    def touch_merged        = (!is_single_output && save_merged) ? "touch ${prefix}.merged.fastq.gz" : ""
+    """
+    touch $touch_reads
+    touch "${prefix}.fastp.json"
+    touch "${prefix}.fastp.html"
+    touch "${prefix}.fastp.log"
+    $touch_merged
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test
index f610b735e..6f1f48978 100644
--- a/modules/nf-core/fastp/tests/main.nf.test
+++ b/modules/nf-core/fastp/tests/main.nf.test
@@ -19,11 +19,10 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:true ],
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-                ]
-
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -57,7 +56,67 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_single_end-_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_single_end") }
+            )
+        }
+    }
+
+    test("test_fastp_single_end-stub") {
+
+        options '-stub'
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
+
+        then {
+
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_single_end-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_single_end_stub") }
             )
         }
     }
@@ -74,12 +133,11 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                ]
-
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -127,13 +185,28 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_paired_end") }
             )
         }
     }
 
-    test("fastp test_fastp_interleaved") {
-        config './nextflow.config'
+    test("test_fastp_paired_end-stub") {
+
+        options '-stub'
+
         when {
             params {
                 outdir   = "$outputDir"
@@ -144,10 +217,56 @@ nextflow_process {
                 save_trimmed_fail = false
                 save_merged       = false
 
-                input[0] = [ [ id:'test', single_end:true ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
-                        ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
 
+        then {
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_paired_end-stub") }
+            )
+        }
+    }
+
+    test("fastp test_fastp_interleaved") {
+
+        config './nextflow.interleaved.config'
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -159,7 +278,7 @@ nextflow_process {
             def html_text  = [ "Q20 bases:</td><td class='col2'>25.719000 K (93.033098%)",
 									"paired end (151 cycles + 151 cycles)"]
             def log_text   = [ "Q20 bases: 12922(92.9841%)",
-									"reads passed filter: 198"]
+									"reads passed filter: 162"]
             def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
 									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
 									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
@@ -181,7 +300,67 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_interleaved-_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_interleaved") }
+            )
+        }
+    }
+
+    test("fastp test_fastp_interleaved-stub") {
+
+        options '-stub'
+
+        config './nextflow.interleaved.config'
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { file(it[1]).getName() } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_interleaved-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_interleaved-stub") }
             )
         }
     }
@@ -198,9 +377,10 @@ nextflow_process {
                 save_trimmed_fail = true
                 save_merged       = false
 
-                input[0] = [ [ id:'test', single_end:true ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
-                        ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -241,13 +421,14 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_single_end_trim_fail") }
             )
         }
     }
 
     test("test_fastp_paired_end_trim_fail") {
 
+        config './nextflow.save_failed.config'
         when {
             params {
                 outdir   = "$outputDir"
@@ -258,13 +439,11 @@ nextflow_process {
                 save_trimmed_fail = true
                 save_merged       = false
 
-                input[0] = [
+                input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
-                    ]
-                ]
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -277,7 +456,7 @@ nextflow_process {
 									"The input has little adapter percentage (~0.000000%), probably it's trimmed before."]
             def log_text            = [ "No adapter detected for read1",
 									"Q30 bases: 12281(88.3716%)"]
-            def json_text           = ['"passed_filter_reads": 198']
+            def json_text           = ['"passed_filter_reads": 162']
             def read1_lines         = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
 									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
 									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
@@ -290,9 +469,9 @@ nextflow_process {
 									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/2",
 									"GCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGT",
 									"AAAAA6EEAEEEEEAEEAEEAEEEEEEA6EEEEAEEAEEEEE6EEEEEEAEEEEA///A<<EEEEEEEEEAEEEEEE"]
-            def failed_read2_lines  = ["@ERR5069949.885966 NS500628:121:HK3MMAFX2:4:11610:19682:20132/2",
-									"CTTAGGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACACAATTTTGCATAGAATGTCCAATAA",
-									"A//AA6EEAEEEEE6EEE/EEA/EA///AAE/EAEEEAE6AE/E/E/EEAAE/EAA/E/E/<EA//E/6"]
+            def failed_read2_lines  = ["@ERR5069949.973930 NS500628:121:HK3MMAFX2:2:21105:5548:5659/2",
+									"CCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAG",
+									"AAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEAE<EAEEEEEEAEEAEE"]
             // def failed_read1_lines = path(process.out.reads_fail.get(0).get(1).get(0)).linesGzip is empty file
             assertAll(
                 { assert process.success },
@@ -306,7 +485,7 @@ nextflow_process {
                     }
                 },
                 { failed_read2_lines.each { failed_read2_line ->
-                    { assert path(process.out.reads_fail.get(0).get(1).get(1)).linesGzip.contains(failed_read2_line) }
+                    { assert path(process.out.reads_fail.get(0).get(1).get(2)).linesGzip.contains(failed_read2_line) }
                     }
                 },
                 { html_text.each { html_part ->
@@ -321,7 +500,7 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") }
             )
         }
     }
@@ -337,11 +516,11 @@ nextflow_process {
                 adapter_fasta     = []
                 save_trimmed_fail = false
                 save_merged       = true
-
-                input[0] = [ [ id:'test', single_end:false ], // meta map
-                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                           ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -399,7 +578,67 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end_merged_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_paired_end_merged") }
+            )
+        }
+    }
+
+    test("test_fastp_paired_end_merged-stub") {
+
+        options '-stub'
+
+        when {
+            params {
+                outdir   = "$outputDir"
+            }
+            process {
+                """
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = true
+
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = adapter_fasta
+                input[2] = save_trimmed_fail
+                input[3] = save_merged
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        (
+                            [process.out.reads[0][0].toString()] + // meta
+                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
+                            process.out.json.collect { file(it[1]).getName() } +
+                            process.out.html.collect { file(it[1]).getName() } +
+                            process.out.log.collect { file(it[1]).getName() } +
+                            process.out.reads_fail.collect { file(it[1]).getName() } +
+                            process.out.reads_merged.collect { file(it[1]).getName() }
+                        ).sort()
+                    ).match("test_fastp_paired_end_merged-for_stub_match")
+                },
+                { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") }
             )
         }
     }
@@ -412,14 +651,15 @@ nextflow_process {
             }
             process {
                 """
-                adapter_fasta     = file("https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/fastp/adapters.fasta", checkIfExists: true)
+                adapter_fasta     = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ])
                 save_trimmed_fail = false
                 save_merged       = true
 
-                input[0] = [ [ id:'test', single_end:false ], // meta map
-                             [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
-                               file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
-                           ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
                 input[1] = adapter_fasta
                 input[2] = save_trimmed_fail
                 input[3] = save_merged
@@ -478,7 +718,7 @@ nextflow_process {
                     { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
                     }
                 },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") }
             )
         }
     }
diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap
index 0fa68c7d7..3e8762889 100644
--- a/modules/nf-core/fastp/tests/main.nf.test.snap
+++ b/modules/nf-core/fastp/tests/main.nf.test.snap
@@ -7,11 +7,47 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.fastp.json:md5,168f516f7bd4b7b6c32da7cba87299a4"
+                    "test.fastp.json:md5,b24e0624df5cc0b11cd5ba21b726fb22"
                 ]
             ]
         ],
-        "timestamp": "2023-10-17T11:04:45.794175881"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:19:15.063001"
+    },
+    "test_fastp_paired_end_merged-for_stub_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "test.merged.fastq.gz",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-17T18:10:13.467574"
+    },
+    "versions_interleaved": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:56:24.615634793"
     },
     "test_fastp_single_end_json": {
         "content": [
@@ -25,15 +61,253 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-17T11:04:10.566343705"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:18:43.526412"
+    },
+    "versions_paired_end": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:55:42.333545689"
+    },
+    "test_fastp_paired_end_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T12:03:06.431833729"
+    },
+    "test_fastp_interleaved-_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:19:15.111894"
+    },
+    "test_fastp_paired_end_merged_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "test.merged.fastq.gz",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T12:08:44.496251446"
+    },
+    "versions_single_end_stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:55:27.354051299"
+    },
+    "versions_interleaved-stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:56:46.535528418"
+    },
+    "versions_single_end_trim_fail": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:03.724591407"
+    },
+    "test_fastp_paired_end-for_stub_match": {
+        "content": [
+            [
+                [
+                    "test_1.fastp.fastq.gz",
+                    "test_2.fastp.fastq.gz"
+                ],
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=false}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-17T18:07:15.398827"
+    },
+    "versions_paired_end-stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:56:06.50017282"
+    },
+    "versions_single_end": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:55:07.67921647"
+    },
+    "versions_paired_end_merged_stub": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:47.350653154"
+    },
+    "test_fastp_interleaved-for_stub_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-17T18:08:06.127974"
+    },
+    "versions_paired_end_trim_fail": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:18.140484878"
+    },
+    "test_fastp_single_end-for_stub_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-17T18:06:00.244202"
+    },
+    "test_fastp_single_end-_match": {
+        "content": [
+            [
+                "test.fastp.fastq.gz",
+                "test.fastp.html",
+                "test.fastp.json",
+                "test.fastp.log",
+                "{id=test, single_end=true}"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:18:43.580336"
+    },
+    "versions_paired_end_merged_adapterlist": {
+        "content": [
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T12:05:37.845370554"
     },
-    "versions": {
+    "versions_paired_end_merged": {
         "content": [
             [
                 "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
-        "timestamp": "2023-10-17T11:04:10.582076024"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:59:32.860543858"
     },
     "test_fastp_single_end_trim_fail_json": {
         "content": [
@@ -47,6 +321,10 @@
                 ]
             ]
         ],
-        "timestamp": "2023-10-17T11:05:00.379878948"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-01-17T18:08:41.942317"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/fastp/tests/nextflow.interleaved.config b/modules/nf-core/fastp/tests/nextflow.interleaved.config
new file mode 100644
index 000000000..4be8dbd2c
--- /dev/null
+++ b/modules/nf-core/fastp/tests/nextflow.interleaved.config
@@ -0,0 +1,5 @@
+process {
+    withName: FASTP {
+        ext.args = "--interleaved_in -e 30"
+    }
+}
diff --git a/modules/nf-core/fastp/tests/nextflow.config b/modules/nf-core/fastp/tests/nextflow.save_failed.config
similarity index 50%
rename from modules/nf-core/fastp/tests/nextflow.config
rename to modules/nf-core/fastp/tests/nextflow.save_failed.config
index 0f7849ad9..53b61b0c1 100644
--- a/modules/nf-core/fastp/tests/nextflow.config
+++ b/modules/nf-core/fastp/tests/nextflow.save_failed.config
@@ -1,6 +1,5 @@
 process {
-
     withName: FASTP {
-        ext.args = "--interleaved_in"
+        ext.args = "-e 30"
     }
 }
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 9e19a74c5..d79f1c862 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -25,6 +25,11 @@ process FASTQC {
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
     def rename_to = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
+
+    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB')
+    // FastQC memory value allowed range (100 - 10000)
+    def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
+
     """
     printf "%s %s\\n" $rename_to | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
@@ -33,6 +38,7 @@ process FASTQC {
     fastqc \\
         $args \\
         --threads $task.cpus \\
+        --memory $fastqc_memory \\
         $renamed_files
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/gatk4/bedtointervallist/environment.yml b/modules/nf-core/gatk4/bedtointervallist/environment.yml
index e7cb4280e..d6fbe2e77 100644
--- a/modules/nf-core/gatk4/bedtointervallist/environment.yml
+++ b/modules/nf-core/gatk4/bedtointervallist/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::gatk4=4.4.0.0
+  - bioconda::gatk4=4.5.0.0
diff --git a/modules/nf-core/gatk4/bedtointervallist/main.nf b/modules/nf-core/gatk4/bedtointervallist/main.nf
index 88b24b1ac..68863d677 100644
--- a/modules/nf-core/gatk4/bedtointervallist/main.nf
+++ b/modules/nf-core/gatk4/bedtointervallist/main.nf
@@ -4,8 +4,8 @@ process GATK4_BEDTOINTERVALLIST {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0':
-        'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0':
+        'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bed)
diff --git a/modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test b/modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test
new file mode 100644
index 000000000..2289f73fa
--- /dev/null
+++ b/modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test
@@ -0,0 +1,38 @@
+nextflow_process {
+
+    name "Test Process GATK4_BEDTOINTERVALLIST"
+    script "../main.nf"
+    process "GATK4_BEDTOINTERVALLIST"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "gatk4"
+    tag "gatk4/bedtointervallist"
+
+    test("test_gatk4_bedtointervallist") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test' ], // meta map
+                [file(params.modules_testdata_base_path + 
+                'genomics/sarscov2/genome/bed/test.bed', checkIfExists: true) ]
+                ]
+                input[1] = [ [ id:'dict' ], // meta map
+                [file(params.modules_testdata_base_path +
+                'genomics/sarscov2/genome/genome.dict', checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test.snap b/modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test.snap
new file mode 100644
index 000000000..48c322fd0
--- /dev/null
+++ b/modules/nf-core/gatk4/bedtointervallist/tests/main.nf.test.snap
@@ -0,0 +1,35 @@
+{
+    "test_gatk4_bedtointervallist": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.interval_list:md5,e51101c9357fb2d59fd30e370eefa39c"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,29a18c36f27584eb5a5f2f5457088b3b"
+                ],
+                "interval_list": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.interval_list:md5,e51101c9357fb2d59fd30e370eefa39c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,29a18c36f27584eb5a5f2f5457088b3b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-19T14:20:12.168775"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/gatk4/bedtointervallist/tests/tags.yml b/modules/nf-core/gatk4/bedtointervallist/tests/tags.yml
new file mode 100644
index 000000000..b4d54f129
--- /dev/null
+++ b/modules/nf-core/gatk4/bedtointervallist/tests/tags.yml
@@ -0,0 +1,2 @@
+gatk4/bedtointervallist:
+  - "modules/nf-core/gatk4/bedtointervallist/**"
diff --git a/modules/nf-core/gatk4/createsequencedictionary/environment.yml b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
index db663e148..78822ad03 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/environment.yml
+++ b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::gatk4=4.4.0.0
+  - bioconda::gatk4=4.5.0.0
diff --git a/modules/nf-core/gatk4/createsequencedictionary/main.nf b/modules/nf-core/gatk4/createsequencedictionary/main.nf
index b47ad1622..c7f1d75b3 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/main.nf
+++ b/modules/nf-core/gatk4/createsequencedictionary/main.nf
@@ -4,8 +4,8 @@ process GATK4_CREATESEQUENCEDICTIONARY {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gatk4:4.4.0.0--py36hdfd78af_0':
-        'biocontainers/gatk4:4.4.0.0--py36hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/gatk4:4.5.0.0--py36hdfd78af_0':
+        'biocontainers/gatk4:4.5.0.0--py36hdfd78af_0' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/gatk4/markduplicates/environment.yml b/modules/nf-core/gatk4/markduplicates/environment.yml
index 9adad104d..7362eea6f 100644
--- a/modules/nf-core/gatk4/markduplicates/environment.yml
+++ b/modules/nf-core/gatk4/markduplicates/environment.yml
@@ -4,5 +4,6 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::gatk4=4.4.0.0
-  - bioconda::samtools=1.17
+  - bioconda::gatk4=4.5.0.0
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/gatk4/markduplicates/main.nf b/modules/nf-core/gatk4/markduplicates/main.nf
index 564b86d3d..baadefef4 100644
--- a/modules/nf-core/gatk4/markduplicates/main.nf
+++ b/modules/nf-core/gatk4/markduplicates/main.nf
@@ -4,8 +4,8 @@ process GATK4_MARKDUPLICATES {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-d9e7bad0f7fbc8f4458d5c3ab7ffaaf0235b59fb:f857e2d6cc88d35580d01cf39e0959a68b83c1d9-0':
-        'biocontainers/mulled-v2-d9e7bad0f7fbc8f4458d5c3ab7ffaaf0235b59fb:f857e2d6cc88d35580d01cf39e0959a68b83c1d9-0' }"
+        'https://depot.galaxyproject.org/singularity/mulled-v2-d9e7bad0f7fbc8f4458d5c3ab7ffaaf0235b59fb:7cc3d06cbf42e28c5e2ebfc7c858654c7340a9d5-0':
+        'biocontainers/mulled-v2-d9e7bad0f7fbc8f4458d5c3ab7ffaaf0235b59fb:7cc3d06cbf42e28c5e2ebfc7c858654c7340a9d5-0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/gatk4/markduplicates/tests/bam.config b/modules/nf-core/gatk4/markduplicates/tests/bam.config
new file mode 100644
index 000000000..0bbfbac35
--- /dev/null
+++ b/modules/nf-core/gatk4/markduplicates/tests/bam.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: GATK4_MARKDUPLICATES {
+        ext.args = '--CREATE_INDEX true'
+        ext.prefix = { "${meta.id}.bam" }
+    }
+
+}
diff --git a/modules/nf-core/gatk4/markduplicates/tests/cram.config b/modules/nf-core/gatk4/markduplicates/tests/cram.config
new file mode 100644
index 000000000..04a9b0745
--- /dev/null
+++ b/modules/nf-core/gatk4/markduplicates/tests/cram.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: GATK4_MARKDUPLICATES {
+        ext.args = '--CREATE_INDEX true'
+        ext.prefix = { "${meta.id}.cram" }
+    }
+
+}
diff --git a/modules/nf-core/gatk4/markduplicates/tests/main.nf.test b/modules/nf-core/gatk4/markduplicates/tests/main.nf.test
new file mode 100644
index 000000000..bbcf74db6
--- /dev/null
+++ b/modules/nf-core/gatk4/markduplicates/tests/main.nf.test
@@ -0,0 +1,126 @@
+nextflow_process {
+
+    name "Test Process GATK4_MARKDUPLICATES"
+    script "../main.nf"
+    process "GATK4_MARKDUPLICATES"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "gatk4"
+    tag "gatk4/markduplicates"
+
+    test("sarscov2 - bam") {
+        config "./bam.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = []
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.bam).match("bam") },
+                { assert snapshot(process.out.bai).match("bai") },
+                { assert snapshot(process.out.versions).match("versions") },
+                { assert snapshot(file(process.out.metrics[0][1]).name).match("test.metrics") }
+            )
+        }
+    }
+
+    test("homo_sapiens - multiple bam") {
+        config "./bam.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ]
+                input[1] = []
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.bam).match("multi bam") },
+                { assert snapshot(process.out.bai).match("multi bai") },
+                { assert snapshot(process.out.versions).match("multi versions") },
+                { assert snapshot(file(process.out.metrics[0][1]).name).match("multi test.metrics") }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - multiple cram") {
+        config "./cram.config"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ]
+                input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.cram[0][1]).name).match("multi cram")},
+                { assert snapshot(file(process.out.crai[0][1]).name).match("multi crai") },
+                { assert snapshot(process.out.versions).match("multi cram versions") },
+                { assert snapshot(file(process.out.metrics[0][1]).name).match("multi cram test.metrics") }
+            )
+        }
+
+    }
+
+    test("stub") {
+        config "./bam.config"
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ],
+                    []
+                ]
+                input[1] = []
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap b/modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap
new file mode 100644
index 000000000..336bb3735
--- /dev/null
+++ b/modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap
@@ -0,0 +1,160 @@
+{
+    "multi bam": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.bam:md5,8a808b1a94d2627c4d659a2151c4cb9f"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-02-13T15:21:36.059923"
+    },
+    "multi crai": {
+        "content": [
+            "test.cram.crai"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2023-12-12T17:43:37.780426007"
+    },
+    "multi bai": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.bai:md5,38b99c5f771895ecf5324c3186b9d452"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-02-13T15:21:36.09642"
+    },
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,c58bf16c6e3786cc4d17bb7249f9ffe5"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-02-13T15:21:08.710549"
+    },
+    "multi test.metrics": {
+        "content": [
+            "test.bam.metrics"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2023-12-12T17:43:11.732892667"
+    },
+    "bai": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.bai:md5,26001bcdbce12e9f07557d8f7b8d360e"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2023-12-12T17:42:39.651888758"
+    },
+    "multi cram versions": {
+        "content": [
+            [
+                "versions.yml:md5,c58bf16c6e3786cc4d17bb7249f9ffe5"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-02-13T15:21:56.966376"
+    },
+    "multi versions": {
+        "content": [
+            [
+                "versions.yml:md5,c58bf16c6e3786cc4d17bb7249f9ffe5"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-02-13T15:21:36.138095"
+    },
+    "multi cram test.metrics": {
+        "content": [
+            "test.cram.metrics"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2023-12-12T17:43:37.798977444"
+    },
+    "multi cram": {
+        "content": [
+            "test.cram"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2023-12-12T17:43:37.771137858"
+    },
+    "bam": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.bam:md5,75d914ba8804eaf2acf02ab432197ec9"
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-02-13T15:21:08.645892"
+    },
+    "test.metrics": {
+        "content": [
+            "test.bam.metrics"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2023-12-12T17:42:39.672508385"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/gatk4/markduplicates/tests/tags.yml b/modules/nf-core/gatk4/markduplicates/tests/tags.yml
new file mode 100644
index 000000000..8632e32b3
--- /dev/null
+++ b/modules/nf-core/gatk4/markduplicates/tests/tags.yml
@@ -0,0 +1,2 @@
+gatk4/markduplicates:
+  - "modules/nf-core/gatk4/markduplicates/**"
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
index 5c85f8725..73ba7dc6b 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
+++ b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::picard=3.1.0
+  - bioconda::picard=3.1.1
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/main.nf b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
index 48e4d2ad2..06c4bc172 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/main.nf
+++ b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTINSERTSIZEMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.1.0--hdfd78af_0' :
-        'biocontainers/picard:3.1.0--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' :
+        'biocontainers/picard:3.1.1--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/meta.yml b/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
index efd5abe06..e2749cb62 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
+++ b/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
@@ -12,7 +12,7 @@ tools:
       homepage: "https://broadinstitute.github.io/picard/"
       documentation: "https://broadinstitute.github.io/picard/"
       tool_dev_url: "https://github.com/broadinstitute/picard"
-      licence: "['MIT']"
+      licence: ["MIT"]
 input:
   - meta:
       type: map
diff --git a/modules/nf-core/picard/collectwgsmetrics/environment.yml b/modules/nf-core/picard/collectwgsmetrics/environment.yml
index 8adda4914..6a0b9258e 100644
--- a/modules/nf-core/picard/collectwgsmetrics/environment.yml
+++ b/modules/nf-core/picard/collectwgsmetrics/environment.yml
@@ -4,5 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::picard=3.1.0
+  - bioconda::picard=3.1.1
   - r::r-base
diff --git a/modules/nf-core/picard/collectwgsmetrics/main.nf b/modules/nf-core/picard/collectwgsmetrics/main.nf
index 67aa5b5e8..35f4129cf 100644
--- a/modules/nf-core/picard/collectwgsmetrics/main.nf
+++ b/modules/nf-core/picard/collectwgsmetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTWGSMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.1.0--hdfd78af_0' :
-        'biocontainers/picard:3.1.0--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' :
+        'biocontainers/picard:3.1.1--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml
index 73badedb1..9c24eb0ab 100644
--- a/modules/nf-core/samtools/faidx/environment.yml
+++ b/modules/nf-core/samtools/faidx/environment.yml
@@ -1,7 +1,10 @@
 name: samtools_faidx
+
 channels:
   - conda-forge
   - bioconda
   - defaults
+
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::htslib=1.19.1
+  - bioconda::samtools=1.19.2
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
index 3aa988224..cfe7ad95f 100644
--- a/modules/nf-core/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_FAIDX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
index e189af28f..f3c25de20 100644
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -39,6 +39,10 @@ output:
       description: |
         Groovy Map containing sample information
         e.g. [ id:'test', single_end:false ]
+  - fa:
+      type: file
+      description: FASTA file
+      pattern: "*.{fa}"
   - fai:
       type: file
       description: FASTA index file
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test b/modules/nf-core/samtools/faidx/tests/main.nf.test
new file mode 100644
index 000000000..17244ef2e
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test
@@ -0,0 +1,122 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_FAIDX"
+    script "../main.nf"
+    process "SAMTOOLS_FAIDX"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/faidx"
+
+    test("test_samtools_faidx") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_bgzip") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)]
+
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_fasta") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_stub_fasta") {
+
+        config "./nextflow2.config"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_stub_fai") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
+
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
new file mode 100644
index 000000000..3e651ef68
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
@@ -0,0 +1,249 @@
+{
+    "test_samtools_faidx": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:22:39.412601"
+    },
+    "test_samtools_faidx_bgzip": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:23:22.427966"
+    },
+    "test_samtools_faidx_fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,6a0774a0ad937ba0bfd2ac7457d90f36"
+                    ]
+                ],
+                "fai": [
+                    
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:24:04.107537"
+    },
+    "test_samtools_faidx_stub_fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "extract.fa:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "fai": [
+                    
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:24:45.868463"
+    },
+    "test_samtools_faidx_stub_fai": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-18T16:25:27.550554"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/tests/nextflow.config b/modules/nf-core/samtools/faidx/tests/nextflow.config
new file mode 100644
index 000000000..f76a3ba09
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/nextflow.config
@@ -0,0 +1,7 @@
+process {
+
+    withName: SAMTOOLS_FAIDX {
+        ext.args = 'MT192765.1 -o extract.fa'
+    }
+
+}
diff --git a/modules/nf-core/samtools/faidx/tests/nextflow2.config b/modules/nf-core/samtools/faidx/tests/nextflow2.config
new file mode 100644
index 000000000..33ebbd5df
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/nextflow2.config
@@ -0,0 +1,6 @@
+process {
+
+    withName: SAMTOOLS_FAIDX {
+        ext.args = '-o extract.fa'
+    }
+}
diff --git a/modules/nf-core/samtools/faidx/tests/tags.yml b/modules/nf-core/samtools/faidx/tests/tags.yml
new file mode 100644
index 000000000..e4a839481
--- /dev/null
+++ b/modules/nf-core/samtools/faidx/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/faidx:
+  - modules/nf-core/samtools/faidx/**
diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml
index 3c6f95b25..a5e506498 100644
--- a/modules/nf-core/samtools/index/environment.yml
+++ b/modules/nf-core/samtools/index/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index 256bd7c46..dc14f98d6 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_INDEX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test
index c76a9169f..bb7756d1c 100644
--- a/modules/nf-core/samtools/index/tests/main.nf.test
+++ b/modules/nf-core/samtools/index/tests/main.nf.test
@@ -8,7 +8,7 @@ nextflow_process {
     tag "samtools"
     tag "samtools/index"
 
-    test("sarscov2 [BAI]") {
+    test("bai") {
 
         when {
             params {
@@ -16,10 +16,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -28,12 +28,12 @@ nextflow_process {
             assertAll (
                 { assert process.success },
                 { assert snapshot(process.out.bai).match("bai") },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.versions).match("bai_versions") }
             )
         }
     }
 
-    test("homo_sapiens [CRAI]") {
+    test("crai") {
 
         when {
             params {
@@ -41,10 +41,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true)
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -53,12 +53,12 @@ nextflow_process {
             assertAll (
                 { assert process.success },
                 { assert snapshot(process.out.crai).match("crai") },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.versions).match("crai_versions") }
             )
         }
     }
 
-    test("homo_sapiens [CSI]") {
+    test("csi") {
 
         config "./csi.nextflow.config"
 
@@ -68,10 +68,10 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -80,7 +80,7 @@ nextflow_process {
             assertAll (
                 { assert process.success },
                 { assert path(process.out.csi.get(0).get(1)).exists() },
-                { assert path(process.out.versions.get(0)).getText().contains("samtools") }
+                { assert snapshot(process.out.versions).match("csi_versions") }
             )
         }
     }
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap
index b3baee7fb..3dc8e7de8 100644
--- a/modules/nf-core/samtools/index/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap
@@ -1,28 +1,74 @@
 {
+    "crai_versions": {
+        "content": [
+            [
+                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:00.324667957"
+    },
+    "csi_versions": {
+        "content": [
+            [
+                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:07.885103162"
+    },
     "crai": {
         "content": [
             [
                 [
                     {
-                        "id": "test"
+                        "id": "test",
+                        "single_end": false
                     },
                     "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
                 ]
             ]
         ],
-        "timestamp": "2023-11-15T15:17:37.30801"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T18:41:38.446424"
     },
     "bai": {
         "content": [
             [
                 [
                     {
-                        "id": "test"
+                        "id": "test",
+                        "single_end": false
                     },
                     "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
                 ]
             ]
         ],
-        "timestamp": "2023-11-15T15:17:30.869234"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T18:40:46.579747"
+    },
+    "bai_versions": {
+        "content": [
+            [
+                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:11:51.641425452"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml
index 508659f02..4d898e486 100644
--- a/modules/nf-core/samtools/sort/environment.yml
+++ b/modules/nf-core/samtools/sort/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index 60f0c634d..fc374f980 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -4,15 +4,18 @@ process SAMTOOLS_SORT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
-    tuple val(meta), path(bam)
+    tuple val(meta) , path(bam)
+    tuple val(meta2), path(fasta)
 
     output:
-    tuple val(meta), path("*.bam"), emit: bam
-    tuple val(meta), path("*.csi"), emit: csi, optional: true
+    tuple val(meta), path("*.bam"),     emit: bam,  optional: true
+    tuple val(meta), path("*.cram"),    emit: cram, optional: true
+    tuple val(meta), path("*.crai"),    emit: crai, optional: true
+    tuple val(meta), path("*.csi"),     emit: csi,  optional: true
     path  "versions.yml"          , emit: versions
 
     when:
@@ -21,14 +24,24 @@ process SAMTOOLS_SORT {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def extension = args.contains("--output-fmt sam") ? "sam" :
+                    args.contains("--output-fmt cram") ? "cram" :
+                    "bam"
+    def reference = fasta ? "--reference ${fasta}" : ""
     if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+
     """
+    samtools cat \\
+        --threads $task.cpus \\
+        ${bam} \\
+    | \\
     samtools sort \\
         $args \\
-        -@ $task.cpus \\
-        -o ${prefix}.bam \\
-        -T $prefix \\
-        $bam
+        -T ${prefix} \\
+        --threads $task.cpus \\
+        ${reference} \\
+        -o ${prefix}.${extension} \\
+        -
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -40,6 +53,7 @@ process SAMTOOLS_SORT {
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.bam
+    touch ${prefix}.bam.csi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml
index 2200de72f..341a7d0eb 100644
--- a/modules/nf-core/samtools/sort/meta.yml
+++ b/modules/nf-core/samtools/sort/meta.yml
@@ -23,8 +23,18 @@ input:
         e.g. [ id:'test', single_end:false ]
   - bam:
       type: file
-      description: BAM/CRAM/SAM file
+      description: BAM/CRAM/SAM file(s)
       pattern: "*.{bam,cram,sam}"
+  - meta2:
+      type: map
+      description: |
+        Groovy Map containing reference information
+        e.g. [ id:'genome' ]
+  - fasta:
+      type: file
+      description: Reference genome FASTA file
+      pattern: "*.{fa,fasta,fna}"
+      optional: true
 output:
   - meta:
       type: map
@@ -33,19 +43,29 @@ output:
         e.g. [ id:'test', single_end:false ]
   - bam:
       type: file
-      description: Sorted BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - versions:
+      description: Sorted BAM file
+      pattern: "*.{bam}"
+  - cram:
       type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      description: Sorted CRAM file
+      pattern: "*.{cram}"
+  - crai:
+      type: file
+      description: CRAM index file (optional)
+      pattern: "*.crai"
   - csi:
       type: file
       description: BAM index file (optional)
       pattern: "*.csi"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@ewels"
+  - "@matthdsm"
 maintainers:
   - "@drpatelh"
   - "@ewels"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test
index 1f72f3b96..8360e2b1f 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test
@@ -8,22 +8,21 @@ nextflow_process {
     tag "samtools"
     tag "samtools/sort"
 
-    test("test_samtools_sort") {
+    test("bam") {
 
         config "./nextflow.config"
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                input[0] = [
-                    [ id:'test', single_end:false ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true)
-                    ]
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -34,13 +33,39 @@ nextflow_process {
                 { assert snapshot(process.out).match() }
             )
         }
+    }
+
+    test("cram") {
+
+        config "./nextflow.config"
 
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
     }
 
-    test("test_samtools_sort_stub") {
+    test("bam_stub") {
 
         config "./nextflow.config"
-        options "-stub-run"
+        options "-stub"
 
         when {
             params {
@@ -48,12 +73,14 @@ nextflow_process {
             }
             process {
                 """
-                input[0] = [
-                    [ id:'test', single_end:false ],
-                    [
-                        file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true)
-                    ]
-                ]
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
                 """
             }
         }
@@ -61,10 +88,9 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") },
+                { assert snapshot(process.out.versions).match("bam_stub_versions") }
             )
         }
-
     }
-
 }
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
index a43566dab..384776564 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "test_samtools_sort": {
+    "cram": {
         "content": [
             {
                 "0": [
@@ -8,14 +8,26 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,a29570e7607d217c2fa4d75829e09cd7"
+                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
                     ]
                 ],
                 "1": [
                     
                 ],
                 "2": [
-                    "versions.yml:md5,46f7a36082fa1f68285fe30d689244e8"
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
                 ],
                 "bam": [
                     [
@@ -23,17 +35,120 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,a29570e7607d217c2fa4d75829e09cd7"
+                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
                     ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
                 ],
                 "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-04T15:08:00.830294"
+    },
+    "bam_stub_bam": {
+        "content": [
+            "test.sorted.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:21:04.364044"
+    },
+    "bam_stub_versions": {
+        "content": [
+            [
+                "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:15:00.20800281"
+    },
+    "bam": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
+                    ]
+                ],
+                "1": [
                     
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                    ]
                 ],
                 "versions": [
-                    "versions.yml:md5,46f7a36082fa1f68285fe30d689244e8"
+                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
                 ]
             }
         ],
-        "timestamp": "2023-10-17T17:21:46.5427968"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-04T15:07:48.773803"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/tests/nextflow.config b/modules/nf-core/samtools/sort/tests/nextflow.config
index d0f350868..f642771f5 100644
--- a/modules/nf-core/samtools/sort/tests/nextflow.config
+++ b/modules/nf-core/samtools/sort/tests/nextflow.config
@@ -1,7 +1,8 @@
 process {
 
     withName: SAMTOOLS_SORT {
-        ext.prefix = { "${meta.id}.sorted" }
+        ext.prefix  = { "${meta.id}.sorted" }
+        ext.args    = "--write-index"
     }
 
 }
diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml
index 141e7bd82..b0676f338 100644
--- a/modules/nf-core/samtools/view/environment.yml
+++ b/modules/nf-core/samtools/view/environment.yml
@@ -4,4 +4,5 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::samtools=1.17
+  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.19.1
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
index ddf3f88ae..5a8989d69 100644
--- a/modules/nf-core/samtools/view/main.nf
+++ b/modules/nf-core/samtools/view/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_VIEW {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' :
-        'biocontainers/samtools:1.17--h00cdaf9_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
+        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input), path(index)
@@ -53,10 +53,19 @@ process SAMTOOLS_VIEW {
     """
 
     stub:
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def file_type = args.contains("--output-fmt sam") ? "sam" :
+                    args.contains("--output-fmt bam") ? "bam" :
+                    args.contains("--output-fmt cram") ? "cram" :
+                    input.getExtension()
+    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+
+    def index = args.contains("--write-index") ? "touch ${prefix}.csi" : ""
+
     """
-    touch ${prefix}.bam
-    touch ${prefix}.cram
+    touch ${prefix}.${file_type}
+    ${index}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/view/tests/bam.config b/modules/nf-core/samtools/view/tests/bam.config
new file mode 100644
index 000000000..c10d10811
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/bam.config
@@ -0,0 +1,3 @@
+process {
+    ext.args = "--output-fmt bam"
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/bam_index.config b/modules/nf-core/samtools/view/tests/bam_index.config
new file mode 100644
index 000000000..771ae033a
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/bam_index.config
@@ -0,0 +1,3 @@
+process {
+    ext.args = "--output-fmt bam --write-index"
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test b/modules/nf-core/samtools/view/tests/main.nf.test
new file mode 100644
index 000000000..45a0defba
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/main.nf.test
@@ -0,0 +1,212 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_VIEW"
+    script "../main.nf"
+    process "SAMTOOLS_VIEW"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/view"
+
+    test("bam") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_bam") },
+                { assert snapshot(process.out.bai).match("bam_bai") },
+                { assert snapshot(process.out.crai).match("bam_crai") },
+                { assert snapshot(process.out.cram).match("bam_cram") },
+                { assert snapshot(process.out.csi).match("bam_csi") },
+                { assert snapshot(process.out.sam).match("bam_sam") },
+                { assert snapshot(process.out.versions).match("bam_versions") }
+            )
+        }
+    }
+
+    test("cram") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.cram[0][1]).name).match("cram_cram") },
+                { assert snapshot(process.out.bai).match("cram_bai") },
+                { assert snapshot(process.out.bam).match("cram_bam") },
+                { assert snapshot(process.out.crai).match("cram_crai") },
+                { assert snapshot(process.out.csi).match("cram_csi") },
+                { assert snapshot(process.out.sam).match("cram_sam") },
+                { assert snapshot(process.out.versions).match("cram_versions") }
+            )
+        }
+    }
+
+    test("cram_to_bam") {
+
+        config "./bam.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    []
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_bam") },
+                { assert snapshot(process.out.bai).match("cram_to_bam_bai") },
+                { assert snapshot(process.out.crai).match("cram_to_bam_crai") },
+                { assert snapshot(process.out.cram).match("cram_to_bam_cram") },
+                { assert snapshot(process.out.csi).match("cram_to_bam_csi") },
+                { assert snapshot(process.out.sam).match("cram_to_bam_sam") },
+                { assert snapshot(process.out.versions).match("cram_to_bam_versions") }
+            )
+        }
+    }
+
+    test("cram_to_bam_index") {
+
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    []
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_bam") },
+                { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_csi") },
+                { assert snapshot(process.out.bai).match("cram_to_bam_index_bai") },
+                { assert snapshot(process.out.crai).match("cram_to_bam_index_crai") },
+                { assert snapshot(process.out.cram).match("cram_to_bam_index_cram") },
+                { assert snapshot(process.out.sam).match("cram_to_bam_index_sam") },
+                { assert snapshot(process.out.versions).match("cram_to_bam_index_versions") }
+            )
+        }
+    }
+
+    test("cram_to_bam_index_qname") {
+
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    []
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = Channel.of("testN:2817", "testN:2814").collectFile(name: "readnames.list", newLine: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_qname_bam") },
+                { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_qname_csi") },
+                { assert snapshot(process.out.bai).match("cram_to_bam_index_qname_bai") },
+                { assert snapshot(process.out.crai).match("cram_to_bam_index_qname_crai") },
+                { assert snapshot(process.out.cram).match("cram_to_bam_index_qname_cram") },
+                { assert snapshot(process.out.sam).match("cram_to_bam_index_qname_sam") },
+                { assert snapshot(process.out.versions).match("cram_to_bam_index_qname_versions") }
+            )
+        }
+    }
+
+    test("bam_stub") {
+
+        options "-stub"
+        config "./bam_index.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true),
+                    []
+                ])
+                input[1] = [[],[]]
+                input[2] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") },
+                { assert snapshot(file(process.out.csi[0][1]).name).match("bam_stub_csi") },
+                { assert snapshot(process.out.bai).match("bam_stub_bai") },
+                { assert snapshot(process.out.crai).match("bam_stub_crai") },
+                { assert snapshot(process.out.cram).match("bam_stub_cram") },
+                { assert snapshot(process.out.sam).match("bam_stub_sam") },
+                { assert snapshot(process.out.versions).match("bam_stub_versions") }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap
new file mode 100644
index 000000000..f55943a7a
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/main.nf.test.snap
@@ -0,0 +1,488 @@
+{
+    "bam_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.256068"
+    },
+    "cram_to_bam_index_csi": {
+        "content": [
+            "test.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.958617"
+    },
+    "bam_stub_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.065301"
+    },
+    "bam_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.258578"
+    },
+    "bam_stub_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.071284"
+    },
+    "bam_stub_versions": {
+        "content": [
+            [
+                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:13:09.713353823"
+    },
+    "cram_to_bam_index_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.972288"
+    },
+    "cram_to_bam_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.999247"
+    },
+    "cram_to_bam_index_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.976457"
+    },
+    "cram_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.497581"
+    },
+    "cram_csi": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.50038"
+    },
+    "cram_to_bam_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.992239"
+    },
+    "cram_to_bam_index_qname_csi": {
+        "content": [
+            "test.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.325496"
+    },
+    "bam_stub_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.079529"
+    },
+    "cram_cram": {
+        "content": [
+            "test.cram"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.490286"
+    },
+    "bam_csi": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.262882"
+    },
+    "cram_to_bam_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.989247"
+    },
+    "cram_to_bam_index_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.967681"
+    },
+    "cram_to_bam_index_qname_versions": {
+        "content": [
+            [
+                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:13:03.935041046"
+    },
+    "cram_to_bam_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.982361"
+    },
+    "cram_to_bam_index_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.95456"
+    },
+    "cram_to_bam_index_versions": {
+        "content": [
+            [
+                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:55.910685496"
+    },
+    "cram_to_bam_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.98601"
+    },
+    "cram_to_bam_versions": {
+        "content": [
+            [
+                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:47.715221169"
+    },
+    "cram_bam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.495512"
+    },
+    "bam_stub_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.076908"
+    },
+    "cram_to_bam_index_qname_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.328458"
+    },
+    "cram_to_bam_index_qname_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.330789"
+    },
+    "cram_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.493129"
+    },
+    "bam_stub_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.074313"
+    },
+    "cram_to_bam_index_qname_bam": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.322874"
+    },
+    "bam_versions": {
+        "content": [
+            [
+                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:31.692607421"
+    },
+    "cram_to_bam_index_qname_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.333248"
+    },
+    "bam_crai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.259774"
+    },
+    "bam_cram": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.261287"
+    },
+    "cram_to_bam_csi": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:04.995454"
+    },
+    "cram_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:56.502625"
+    },
+    "cram_versions": {
+        "content": [
+            [
+                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.01.0"
+        },
+        "timestamp": "2024-02-13T16:12:39.913411036"
+    },
+    "bam_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:37:51.264651"
+    },
+    "cram_to_bam_index_bai": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:12.962863"
+    },
+    "cram_to_bam_index_qname_sam": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.337634"
+    },
+    "bam_stub_csi": {
+        "content": [
+            "test.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:32.068596"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/tags.yml b/modules/nf-core/samtools/view/tests/tags.yml
new file mode 100644
index 000000000..4fdf1dd12
--- /dev/null
+++ b/modules/nf-core/samtools/view/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/view:
+  - "modules/nf-core/samtools/view/**"
diff --git a/modules/nf-core/star/align/environment.yml b/modules/nf-core/star/align/environment.yml
index 6db209884..8bd58cff5 100644
--- a/modules/nf-core/star/align/environment.yml
+++ b/modules/nf-core/star/align/environment.yml
@@ -5,5 +5,6 @@ channels:
   - defaults
 dependencies:
   - bioconda::star=2.7.10a
-  - bioconda::samtools=1.16.1
+  - bioconda::samtools=1.18
+  - bioconda::htslib=1.18
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index cc4f5af52..8e9c48b1c 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -4,8 +4,8 @@ process STAR_ALIGN {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:019f262d90511939dce2dca4b7c868fc108f73db-0' :
-        'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:019f262d90511939dce2dca4b7c868fc108f73db-0' }"
+        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' :
+        'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' }"
 
     input:
     tuple val(meta), path(reads, stageAs: "input*/*")
diff --git a/modules/nf-core/star/align/tests/main.nf.test b/modules/nf-core/star/align/tests/main.nf.test
index 4c8784741..6ecd77863 100644
--- a/modules/nf-core/star/align/tests/main.nf.test
+++ b/modules/nf-core/star/align/tests/main.nf.test
@@ -7,39 +7,40 @@ nextflow_process {
     tag "modules_nfcore"
     tag "star"
     tag "star/align"
+    tag "star/genomegenerate"
 
-    test("homo_sapiens - single_end") {
-        config "./nextflow.config"
-
-        setup {
-            run("STAR_GENOMEGENERATE") {
-                script "../../../star/genomegenerate/main.nf"
-                process {
-                    """
-                    input[0] = Channel.of([
-                        [ id:'test_fasta' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]
-                    ])
-                    input[1] = Channel.of([
-                        [ id:'test_gtf' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
-                    ])
-                    """
-                }
+    setup {
+        run("STAR_GENOMEGENERATE") {
+            script "../../../star/genomegenerate/main.nf"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
             }
         }
+    }
+
+    test("homo_sapiens - single_end") {
+        config "./nextflow.config"
 
         when {
             process {
                 """
                 input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
-                    [ file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true) ]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true) ]
                 ])
                 input[1] = STAR_GENOMEGENERATE.out.index
                 input[2] = Channel.of([
                     [ id:'test_gtf' ],
-                    [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
                 ])
                 input[3] = false
                 input[4] = 'illumina'
@@ -74,38 +75,20 @@ nextflow_process {
     test("homo_sapiens - paired_end") {
         config "./nextflow.config"
 
-        setup {
-            run("STAR_GENOMEGENERATE") {
-                script "../../../star/genomegenerate/main.nf"
-                process {
-                    """
-                    input[0] = Channel.of([
-                        [ id:'test_fasta' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]
-                    ])
-                    input[1] = Channel.of([
-                        [ id:'test_gtf' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
-                    ])
-                    """
-                }
-            }
-        }
-
         when {
             process {
                 """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true)
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
                     ]
                 ])
                 input[1] = STAR_GENOMEGENERATE.out.index
                 input[2] = Channel.of([
                     [ id:'test_gtf' ],
-                    [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
                 ])
                 input[3] = false
                 input[4] = 'illumina'
@@ -140,38 +123,20 @@ nextflow_process {
     test("homo_sapiens - paired_end - arriba") {
         config "./nextflow.arriba.config"
 
-        setup {
-            run("STAR_GENOMEGENERATE") {
-                script "../../../star/genomegenerate/main.nf"
-                process {
-                    """
-                    input[0] = Channel.of([
-                        [ id:'test_fasta' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]
-                    ])
-                    input[1] = Channel.of([
-                        [ id:'test_gtf' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
-                    ])
-                    """
-                }
-            }
-        }
-
         when {
             process {
                 """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true)
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
                     ]
                 ])
                 input[1] = STAR_GENOMEGENERATE.out.index
                 input[2] = Channel.of([
                     [ id:'test_gtf' ],
-                    [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
                 ])
                 input[3] = false
                 input[4] = 'illumina'
@@ -206,38 +171,20 @@ nextflow_process {
     test("homo_sapiens - paired_end - starfusion") {
         config "./nextflow.starfusion.config"
 
-        setup {
-            run("STAR_GENOMEGENERATE") {
-                script "../../../star/genomegenerate/main.nf"
-                process {
-                    """
-                    input[0] = Channel.of([
-                        [ id:'test_fasta' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]
-                    ])
-                    input[1] = Channel.of([
-                        [ id:'test_gtf' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
-                    ])
-                    """
-                }
-            }
-        }
-
         when {
             process {
                 """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true)
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
                     ]
                 ])
                 input[1] = STAR_GENOMEGENERATE.out.index
                 input[2] = Channel.of([
                     [ id:'test_gtf' ],
-                    [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
                 ])
                 input[3] = false
                 input[4] = 'illumina'
@@ -272,40 +219,22 @@ nextflow_process {
     test("homo_sapiens - paired_end - multiple") {
         config "./nextflow.config"
 
-        setup {
-            run("STAR_GENOMEGENERATE") {
-                script "../../../star/genomegenerate/main.nf"
-                process {
-                    """
-                    input[0] = Channel.of([
-                        [ id:'test_fasta' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]
-                    ])
-                    input[1] = Channel.of([
-                        [ id:'test_gtf' ],
-                        [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
-                    ])
-                    """
-                }
-            }
-        }
-
         when {
             process {
                 """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true),
-                        file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true)
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
                     ]
                 ])
                 input[1] = STAR_GENOMEGENERATE.out.index
                 input[2] = Channel.of([
                     [ id:'test_gtf' ],
-                    [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
                 ])
                 input[3] = false
                 input[4] = 'illumina'
diff --git a/modules/nf-core/star/align/tests/main.nf.test.snap b/modules/nf-core/star/align/tests/main.nf.test.snap
index 59b735d44..08edb914b 100644
--- a/modules/nf-core/star/align/tests/main.nf.test.snap
+++ b/modules/nf-core/star/align/tests/main.nf.test.snap
@@ -11,7 +11,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:29:01.19639"
+        "timestamp": "2023-12-04T18:01:19.968225733"
     },
     "homo_sapiens - paired_end - multiple - wig": {
         "content": [
@@ -33,7 +33,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:25:07.396223"
+        "timestamp": "2023-12-04T17:56:12.347549723"
     },
     "homo_sapiens - single_end - wig": {
         "content": [
@@ -54,10 +54,10 @@
     "homo_sapiens - paired_end - arriba - versions": {
         "content": [
             [
-                "versions.yml:md5,452ef035aacbc68d47041e86279a9333"
+                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
             ]
         ],
-        "timestamp": "2023-11-23T13:25:07.494015"
+        "timestamp": "2023-12-04T17:56:12.431212643"
     },
     "homo_sapiens - paired_end - multiple - bedgraph": {
         "content": [
@@ -74,7 +74,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:29:01.396383"
+        "timestamp": "2023-12-04T18:01:20.07119229"
     },
     "homo_sapiens - paired_end - read_per_gene_tab": {
         "content": [
@@ -112,7 +112,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:25:07.348239"
+        "timestamp": "2023-12-04T17:56:12.268388251"
     },
     "homo_sapiens - single_end - sam": {
         "content": [
@@ -133,10 +133,10 @@
     "homo_sapiens - single_end - versions": {
         "content": [
             [
-                "versions.yml:md5,452ef035aacbc68d47041e86279a9333"
+                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
             ]
         ],
-        "timestamp": "2023-11-23T13:22:55.259282"
+        "timestamp": "2023-12-04T17:53:26.664210196"
     },
     "homo_sapiens - paired_end - multiple - log_out": {
         "content": [
@@ -172,7 +172,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:29:01.729175"
+        "timestamp": "2023-12-04T18:01:20.189486201"
     },
     "homo_sapiens - paired_end - starfusion - log_final": {
         "content": [
@@ -214,7 +214,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:27:56.003675"
+        "timestamp": "2023-12-04T17:59:58.53235164"
     },
     "homo_sapiens - paired_end - arriba - junction": {
         "content": [
@@ -239,7 +239,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:22:55.163495"
+        "timestamp": "2023-12-04T17:53:26.394863748"
     },
     "homo_sapiens - paired_end - arriba - read_per_gene_tab": {
         "content": [
@@ -277,7 +277,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:23:33.265265"
+        "timestamp": "2023-12-04T17:54:11.934832258"
     },
     "homo_sapiens - paired_end - arriba - bam_transcript": {
         "content": [
@@ -302,10 +302,10 @@
     "homo_sapiens - paired_end - multiple - versions": {
         "content": [
             [
-                "versions.yml:md5,452ef035aacbc68d47041e86279a9333"
+                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
             ]
         ],
-        "timestamp": "2023-11-23T13:29:01.937182"
+        "timestamp": "2023-12-04T18:01:20.393705142"
     },
     "homo_sapiens - paired_end - starfusion - bam_transcript": {
         "content": [
@@ -327,7 +327,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:27:56.379367"
+        "timestamp": "2023-12-04T17:59:58.818041322"
     },
     "homo_sapiens - single_end - fastq": {
         "content": [
@@ -349,7 +349,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:23:33.413683"
+        "timestamp": "2023-12-04T17:54:12.255481058"
     },
     "homo_sapiens - paired_end - starfusion - bedgraph": {
         "content": [
@@ -370,10 +370,10 @@
     "homo_sapiens - paired_end - versions": {
         "content": [
             [
-                "versions.yml:md5,452ef035aacbc68d47041e86279a9333"
+                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
             ]
         ],
-        "timestamp": "2023-11-23T13:23:33.445323"
+        "timestamp": "2023-12-04T17:54:12.343840482"
     },
     "homo_sapiens - paired_end - multiple - tab": {
         "content": [
@@ -387,7 +387,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:29:01.793129"
+        "timestamp": "2023-12-04T18:01:20.291692062"
     },
     "homo_sapiens - single_end - bam": {
         "content": [
@@ -401,7 +401,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:22:55.128568"
+        "timestamp": "2023-12-04T17:53:26.265642675"
     },
     "homo_sapiens - paired_end - arriba - wig": {
         "content": [
@@ -423,7 +423,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:23:33.354416"
+        "timestamp": "2023-12-04T17:54:12.126063825"
     },
     "homo_sapiens - paired_end - arriba - log_final": {
         "content": [
@@ -459,7 +459,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:29:01.13168"
+        "timestamp": "2023-12-04T18:01:19.851247126"
     },
     "homo_sapiens - paired_end - multiple - fastq": {
         "content": [
@@ -481,7 +481,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:22:55.134799"
+        "timestamp": "2023-12-04T17:53:26.335457371"
     },
     "homo_sapiens - paired_end - arriba - bam_sorted": {
         "content": [
@@ -503,7 +503,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:27:56.228327"
+        "timestamp": "2023-12-04T17:59:58.641115828"
     },
     "homo_sapiens - single_end - tab": {
         "content": [
@@ -517,15 +517,15 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:22:55.236346"
+        "timestamp": "2023-12-04T17:53:26.580593434"
     },
     "homo_sapiens - paired_end - starfusion - versions": {
         "content": [
             [
-                "versions.yml:md5,452ef035aacbc68d47041e86279a9333"
+                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
             ]
         ],
-        "timestamp": "2023-11-23T13:27:56.460903"
+        "timestamp": "2023-12-04T17:59:58.907317103"
     },
     "homo_sapiens - paired_end - multiple - bam_unsorted": {
         "content": [
@@ -556,7 +556,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:23:33.313258"
+        "timestamp": "2023-12-04T17:54:12.064121304"
     },
     "homo_sapiens - paired_end - starfusion - bam_unsorted": {
         "content": [
@@ -600,7 +600,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:23:33.274809"
+        "timestamp": "2023-12-04T17:54:12.002180537"
     },
     "homo_sapiens - single_end - spl_junc_tab": {
         "content": [
@@ -614,7 +614,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:22:55.226143"
+        "timestamp": "2023-12-04T17:53:26.50932751"
     },
     "homo_sapiens - paired_end - starfusion - spl_junc_tab": {
         "content": [
@@ -628,7 +628,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:27:56.337072"
+        "timestamp": "2023-12-04T17:59:58.731699486"
     },
     "homo_sapiens - single_end - log_out": {
         "content": [
@@ -706,7 +706,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:23:33.398603"
+        "timestamp": "2023-12-04T17:54:12.185730856"
     },
     "homo_sapiens - paired_end - starfusion - sam": {
         "content": [
@@ -728,7 +728,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:25:06.887604"
+        "timestamp": "2023-12-04T17:56:12.190560178"
     },
     "homo_sapiens - single_end - log_progress": {
         "content": [
@@ -742,7 +742,7 @@
                 ]
             ]
         ],
-        "timestamp": "2023-11-23T13:22:55.195544"
+        "timestamp": "2023-12-04T17:53:26.450352138"
     },
     "homo_sapiens - paired_end - starfusion - wig": {
         "content": [
diff --git a/modules/nf-core/star/genomegenerate/environment.yml b/modules/nf-core/star/genomegenerate/environment.yml
index 0b35ff518..791f255e5 100644
--- a/modules/nf-core/star/genomegenerate/environment.yml
+++ b/modules/nf-core/star/genomegenerate/environment.yml
@@ -4,6 +4,7 @@ channels:
   - bioconda
   - defaults
 dependencies:
+  - bioconda::samtools=1.18
+  - bioconda::htslib=1.18
   - bioconda::star=2.7.10a
-  - bioconda::samtools=1.16.1
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/genomegenerate/main.nf b/modules/nf-core/star/genomegenerate/main.nf
index d20618443..b8855715b 100644
--- a/modules/nf-core/star/genomegenerate/main.nf
+++ b/modules/nf-core/star/genomegenerate/main.nf
@@ -4,8 +4,8 @@ process STAR_GENOMEGENERATE {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:019f262d90511939dce2dca4b7c868fc108f73db-0' :
-        'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:019f262d90511939dce2dca4b7c868fc108f73db-0' }"
+        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' :
+        'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' }"
 
     input:
     tuple val(meta), path(fasta)
@@ -19,9 +19,10 @@ process STAR_GENOMEGENERATE {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def args_list = args.tokenize()
-    def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
+    def args        = task.ext.args ?: ''
+    def args_list   = args.tokenize()
+    def memory      = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
+    def include_gtf = gtf ? "--sjdbGTFfile $gtf" : ''
     if (args_list.contains('--genomeSAindexNbases')) {
         """
         mkdir star
@@ -29,7 +30,7 @@ process STAR_GENOMEGENERATE {
             --runMode genomeGenerate \\
             --genomeDir star/ \\
             --genomeFastaFiles $fasta \\
-            --sjdbGTFfile $gtf \\
+            $include_gtf \\
             --runThreadN $task.cpus \\
             $memory \\
             $args
@@ -51,7 +52,7 @@ process STAR_GENOMEGENERATE {
             --runMode genomeGenerate \\
             --genomeDir star/ \\
             --genomeFastaFiles $fasta \\
-            --sjdbGTFfile $gtf \\
+            $include_gtf \\
             --runThreadN $task.cpus \\
             --genomeSAindexNbases \$NUM_BASES \\
             $memory \\
@@ -67,30 +68,52 @@ process STAR_GENOMEGENERATE {
     }
 
     stub:
-    """
-    mkdir star
-    touch star/Genome
-    touch star/Log.out
-    touch star/SA
-    touch star/SAindex
-    touch star/chrLength.txt
-    touch star/chrName.txt
-    touch star/chrNameLength.txt
-    touch star/chrStart.txt
-    touch star/exonGeTrInfo.tab
-    touch star/exonInfo.tab
-    touch star/geneInfo.tab
-    touch star/genomeParameters.txt
-    touch star/sjdbInfo.txt
-    touch star/sjdbList.fromGTF.out.tab
-    touch star/sjdbList.out.tab
-    touch star/transcriptInfo.tab
+    if (gtf) {
+        """
+        mkdir star
+        touch star/Genome
+        touch star/Log.out
+        touch star/SA
+        touch star/SAindex
+        touch star/chrLength.txt
+        touch star/chrName.txt
+        touch star/chrNameLength.txt
+        touch star/chrStart.txt
+        touch star/exonGeTrInfo.tab
+        touch star/exonInfo.tab
+        touch star/geneInfo.tab
+        touch star/genomeParameters.txt
+        touch star/sjdbInfo.txt
+        touch star/sjdbList.fromGTF.out.tab
+        touch star/sjdbList.out.tab
+        touch star/transcriptInfo.tab
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        star: \$(STAR --version | sed -e "s/STAR_//g")
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//')
-    END_VERSIONS
-    """
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            star: \$(STAR --version | sed -e "s/STAR_//g")
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+            gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//')
+        END_VERSIONS
+        """
+    } else {
+        """
+        mkdir star
+        touch star/Genome
+        touch star/Log.out
+        touch star/SA
+        touch star/SAindex
+        touch star/chrLength.txt
+        touch star/chrName.txt
+        touch star/chrNameLength.txt
+        touch star/chrStart.txt
+        touch star/genomeParameters.txt
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            star: \$(STAR --version | sed -e "s/STAR_//g")
+            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+            gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//')
+        END_VERSIONS
+        """
+    }
 }
diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test b/modules/nf-core/star/genomegenerate/tests/main.nf.test
index eed82926f..c17c8ba45 100644
--- a/modules/nf-core/star/genomegenerate/tests/main.nf.test
+++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test
@@ -8,18 +8,18 @@ nextflow_process {
     tag "star"
     tag "star/genomegenerate"
 
-    test("homo_sapiens") {
+    test("fasta_gtf") {
 
         when {
             process {
                 """
                 input[0] = Channel.of([
                     [ id:'test_fasta' ],
-                    [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
                 ])
                 input[1] = Channel.of([
                     [ id:'test_gtf' ],
-                    [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)]
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
                 ])
                 """
             }
@@ -28,11 +28,88 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.index[0][1]).name).match("index") },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_index") },
+                { assert snapshot(process.out.versions).match("fasta_gtf_versions") }
+            )
+        }
+    }
+
+    test("fasta_gtf_stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_stub_index") },
+                { assert snapshot(process.out.versions).match("fasta_gtf_stub_versions") }
+            )
+        }
+    }
+
+    test("fasta") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([ [], [] ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_index") },
+                { assert snapshot(process.out.versions).match("fasta_versions") }
+            )
+        }
+
+    }
+
+    test("fasta_stub") {
+
+        options '-stub'
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([ [], [] ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_stub_index") },
+                { assert snapshot(process.out.versions).match("fasta_stub_versions") }
             )
         }
 
     }
 
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
index bd4e0caa6..5653d6e6c 100644
--- a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
+++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
@@ -1,16 +1,90 @@
 {
-    "versions": {
+    "fasta_gtf_versions": {
         "content": [
             [
-                "versions.yml:md5,9c11319b80fdedc90dadce4e0fb42ded"
+                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
             ]
         ],
-        "timestamp": "2023-11-23T11:18:14.835118"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:54:31.798555"
     },
-    "index": {
+    "fasta_stub_versions": {
         "content": [
-            "star"
+            [
+                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:55:07.521209"
+    },
+    "fasta_gtf_stub_index": {
+        "content": [
+            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:54:46.478098"
+    },
+    "fasta_gtf_stub_versions": {
+        "content": [
+            [
+                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:54:46.491657"
+    },
+    "fasta_index": {
+        "content": [
+            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:54:57.552329"
+    },
+    "fasta_versions": {
+        "content": [
+            [
+                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:54:57.560541"
+    },
+    "fasta_gtf_index": {
+        "content": [
+            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:54:31.786814"
+    },
+    "fasta_stub_index": {
+        "content": [
+            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]"
         ],
-        "timestamp": "2023-11-23T11:31:47.560528"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-01T15:55:07.517472"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/stringtie/merge/tests/main.nf.test b/modules/nf-core/stringtie/merge/tests/main.nf.test
index 90368134b..358c561a0 100644
--- a/modules/nf-core/stringtie/merge/tests/main.nf.test
+++ b/modules/nf-core/stringtie/merge/tests/main.nf.test
@@ -7,30 +7,30 @@ nextflow_process {
     tag "modules_nfcore"
     tag "stringtie"
     tag "stringtie/merge"
+    tag "stringtie/stringtie"
 
-    test("homo_sapiens - forward strandedness") {
-
-        setup {
-            run("STRINGTIE_STRINGTIE") {
-                script "../../stringtie/main.nf"
-                process {
-                """
-                input[0] = [
-                                [ id:'test', strandedness:'forward' ], // meta map
-                                [ file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
-                            ]
-                input[1] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
-                """
-            }
+    setup {
+        run("STRINGTIE_STRINGTIE") {
+            script "../../stringtie/main.nf"
+            process {
+            """
+            input[0] = [
+                        [ id:'test', strandedness:'reverse' ], // meta map
+                        [ file(params.modules_testdata_base_path + "genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+            input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
+            """
             }
-
         }
+    }
+
+    test("homo_sapiens - forward strandedness") {
 
         when {
             process {
                 """
                 input[0] = STRINGTIE_STRINGTIE.out.transcript_gtf.map { it -> it[1] }
-                input[1] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
+                input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
                 """
             }
         }
@@ -46,22 +46,6 @@ nextflow_process {
 
     test("homo_sapiens - reverse strandedness") {
 
-        setup {
-            run("STRINGTIE_STRINGTIE") {
-                script "../../stringtie/main.nf"
-                process {
-                """
-                input[0] = [
-                                [ id:'test', strandedness:'reverse' ], // meta map
-                                [ file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
-                            ]
-                input[1] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
-                """
-            }
-            }
-
-        }
-
         when {
             process {
                 """
diff --git a/modules/nf-core/stringtie/merge/tests/main.nf.test.snap b/modules/nf-core/stringtie/merge/tests/main.nf.test.snap
index 3e4bc68f6..e10406964 100644
--- a/modules/nf-core/stringtie/merge/tests/main.nf.test.snap
+++ b/modules/nf-core/stringtie/merge/tests/main.nf.test.snap
@@ -5,6 +5,10 @@
                 "versions.yml:md5,b73d45fdebf4c8c446bb01817db1665d"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-11-23T14:14:39.697712988"
     },
     "rs_gtf": {
@@ -13,15 +17,23 @@
                 "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-11-23T14:14:39.691894799"
     },
     "fs_gtf": {
         "content": [
             [
-                "stringtie.merged.gtf:md5,d959eb2fab0db48ded7275e0a2e83c05"
+                "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
             ]
         ],
-        "timestamp": "2023-11-23T14:14:20.872841278"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-07T16:43:48.130184"
     },
     "fs_versions": {
         "content": [
@@ -29,6 +41,10 @@
                 "versions.yml:md5,b73d45fdebf4c8c446bb01817db1665d"
             ]
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
         "timestamp": "2023-11-23T14:14:20.883140097"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/stringtie/stringtie/tests/main.nf.test b/modules/nf-core/stringtie/stringtie/tests/main.nf.test
index 68786b745..00efe8f1a 100644
--- a/modules/nf-core/stringtie/stringtie/tests/main.nf.test
+++ b/modules/nf-core/stringtie/stringtie/tests/main.nf.test
@@ -10,13 +10,15 @@ nextflow_process {
 
     test("sarscov2 [bam] - forward strandedness") {
 
+        config "./nextflow.config"
+
         when {
             process {
                 """
                 input[0] = [
                             [ id:'test', strandedness:'forward' ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
-                           ]
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
                 input[1] = []
                 """
             }
@@ -24,24 +26,26 @@ nextflow_process {
 
         then {
             assertAll(
-            { assert process.success },
-            { assert snapshot(process.out.transcript_gtf).match("fs_transcript_gtf") },
-            { assert snapshot(process.out.abundance).match("fs_abundance") },
-            { assert snapshot(process.out.versions).match("fs_versions") }
+                { assert process.success },
+                { assert snapshot(process.out.transcript_gtf).match("fs_transcript_gtf") },
+                { assert snapshot(process.out.abundance).match("fs_abundance") },
+                { assert snapshot(process.out.versions).match("fs_versions") }
             )
         }
     }
 
     test("sarscov2 [bam] - forward strandedness + reference annotation") {
 
+        config "./nextflow.config"
+
         when {
             process {
                 """
                 input[0] = [
-                            [ id:'test', strandedness:'forward' ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
-                           ]
-                input[1] = file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true)
+                    [ id:'test', strandedness:'forward' ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
                 """
             }
         }
@@ -49,23 +53,25 @@ nextflow_process {
         then {
             assertAll(
             { assert process.success },
-            { assert snapshot(process.out.transcript_gtf).match("fs_gtf_transcript_gtf") },
-            { assert snapshot(process.out.abundance).match("fs_gtf_abundance") },
-            { assert snapshot(process.out.ballgown).match("fs_gtf_ballgown") },
-            { assert snapshot(process.out.versions).match("fs_gtf_versions") }
+                { assert snapshot(process.out.transcript_gtf).match("fs_gtf_transcript_gtf") },
+                { assert snapshot(process.out.abundance).match("fs_gtf_abundance") },
+                { assert snapshot(process.out.ballgown).match("fs_gtf_ballgown") },
+                { assert snapshot(process.out.versions).match("fs_gtf_versions") }
             )
         }
     }
 
     test("sarscov2 [bam] - reverse strandedness") {
 
+        config "./nextflow.config"
+
         when {
             process {
                 """
                 input[0] = [
                             [ id:'test', strandedness:'reverse' ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
-                           ]
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
                 input[1] = []
                 """
             }
@@ -73,35 +79,37 @@ nextflow_process {
 
         then {
             assertAll(
-            { assert process.success },
-            { assert snapshot(process.out.transcript_gtf).match("rs_transcript_gtf") },
-            { assert snapshot(process.out.abundance).match("rs_abundance") },
-            { assert snapshot(process.out.versions).match("rs_versions") }
+                { assert process.success },
+                { assert snapshot(process.out.transcript_gtf).match("rs_transcript_gtf") },
+                { assert snapshot(process.out.abundance).match("rs_abundance") },
+                { assert snapshot(process.out.versions).match("rs_versions") }
             )
         }
     }
 
     test("sarscov2 [bam] - reverse strandedness + reference annotation") {
 
+        config "./nextflow.config"
+
         when {
             process {
                 """
                 input[0] = [
                             [ id:'test', strandedness:'reverse' ], // meta map
-                            [ file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
-                           ]
-                input[1] = file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true)
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
                 """
             }
         }
 
         then {
             assertAll(
-            { assert process.success },
-            { assert snapshot(process.out.transcript_gtf).match("rs_gtf_transcript_gtf") },
-            { assert snapshot(process.out.abundance).match("rs_gtf_abundance") },
-            { assert snapshot(process.out.ballgown).match("rs_gtf_ballgown") },
-            { assert snapshot(process.out.versions).match("rs_gtf_versions") }
+                { assert process.success },
+                { assert snapshot(process.out.transcript_gtf).match("rs_gtf_transcript_gtf") },
+                { assert snapshot(process.out.abundance).match("rs_gtf_abundance") },
+                { assert snapshot(process.out.ballgown).match("rs_gtf_ballgown") },
+                { assert snapshot(process.out.versions).match("rs_gtf_versions") }
             )
         }
     }
diff --git a/modules/nf-core/stringtie/stringtie/tests/nextflow.config b/modules/nf-core/stringtie/stringtie/tests/nextflow.config
new file mode 100644
index 000000000..e3aaa0999
--- /dev/null
+++ b/modules/nf-core/stringtie/stringtie/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'STRINGTIE_STRINGTIE' {
+        ext.args = ''
+    }
+}
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index f59018dc9..3cb8d6ac4 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -1,4 +1,4 @@
-include { ARRIBA }                                      from '../../modules/nf-core/arriba/main'
+include { ARRIBA_ARRIBA }                               from '../../modules/nf-core/arriba/main'
 include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_ARRIBA}  from '../../modules/nf-core/samtools/index/main'
 include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA}    from '../../modules/nf-core/samtools/sort/main'
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_ARRIBA}    from '../../modules/nf-core/samtools/view/main'
@@ -28,16 +28,16 @@ workflow ARRIBA_WORKFLOW {
                     .map { meta, reads, fusions -> [ meta, fusions ] }
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
-                ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, ch_arriba_ref_blacklist, ch_arriba_ref_known_fusions, [[],[]], [[],[]], ch_arriba_ref_protein_domains )
-                ch_versions = ch_versions.mix(ARRIBA.out.versions)
+                ARRIBA_ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, ch_arriba_ref_blacklist, ch_arriba_ref_known_fusions, [[],[]], [[],[]], ch_arriba_ref_protein_domains )
+                ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
-                ch_arriba_fusions     = ARRIBA.out.fusions
-                ch_arriba_fusion_fail = ARRIBA.out.fusions_fail.map{ meta, file -> return file}
+                ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
+                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ meta, file -> return file}
             }
 
             if (params.cram.contains('arriba') ){
 
-                SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam)
+                SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam, ch_fasta)
                 ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions )
 
                 SAMTOOLS_VIEW_FOR_ARRIBA(SAMTOOLS_SORT_FOR_ARRIBA.out.bam.map { meta, bam -> [ meta, bam, [] ] }, ch_fasta, [])
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 7847dd055..bd985e4fb 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -4,7 +4,7 @@
 ========================================================================================
 */
 
-include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
+include { ARRIBA_DOWNLOAD }                 from '../modules/nf-core/arriba/download/main'
 include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'

From 213382715db3a90bdab4b9a9c736e29150f1ba17 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Apr 2024 13:00:08 +0200
Subject: [PATCH 003/887] fix path

---
 subworkflows/local/arriba_workflow.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 3cb8d6ac4..ce142a749 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -1,4 +1,4 @@
-include { ARRIBA_ARRIBA }                               from '../../modules/nf-core/arriba/main'
+include { ARRIBA_ARRIBA }                               from '../../modules/nf-core/arriba/arriba/main'
 include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_ARRIBA}  from '../../modules/nf-core/samtools/index/main'
 include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA}    from '../../modules/nf-core/samtools/sort/main'
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_ARRIBA}    from '../../modules/nf-core/samtools/view/main'

From a8f87e7fcf812780646e26b14703cff5e777bf1f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Apr 2024 13:07:05 +0200
Subject: [PATCH 004/887] add latest zenodo

---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 58189dc64..b418192ac 100644
--- a/README.md
+++ b/README.md
@@ -6,7 +6,7 @@
 </h1>
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)
-[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
+[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.2565517-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.2565517)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
 [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/)

From 6c0645a2c121dd96e86e8bc745ab8b78c724a1c0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Apr 2024 13:21:01 +0200
Subject: [PATCH 005/887] remove TODOs

---
 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 3a02b058b..3aee5fc24 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -183,7 +183,6 @@ def genomeExistsError() {
 // Generate methods description for MultiQC
 //
 def toolCitationText() {
-    // TODO nf-core: Optionally add in-text citation tools to this list.
     // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "",
     // Uncomment function in methodsDescriptionText to render in MultiQC report
     def citation_text = [
@@ -197,7 +196,6 @@ def toolCitationText() {
 }
 
 def toolBibliographyText() {
-    // TODO nf-core: Optionally add bibliographic entries to this list.
     // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "<li>Author (2023) Pub name, Journal, DOI</li>" : "",
     // Uncomment function in methodsDescriptionText to render in MultiQC report
     def reference_text = [
@@ -222,7 +220,7 @@ def methodsDescriptionText(mqc_methods_yaml) {
     meta["tool_citations"] = ""
     meta["tool_bibliography"] = ""
 
-    // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
+    // nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
     // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".")
     // meta["tool_bibliography"] = toolBibliographyText()
 

From 0443c44576219718c3474711ff83db4081233185 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Apr 2024 13:28:56 +0200
Subject: [PATCH 006/887] update starfusion

---
 modules/local/fusioninspector/main.nf     | 4 ++--
 modules/local/starfusion/build/main.nf    | 4 ++--
 modules/local/starfusion/detect/main.nf   | 4 ++--
 modules/local/starfusion/download/main.nf | 4 ++--
 4 files changed, 8 insertions(+), 8 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index c7fcd3f04..2f99c7111 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -2,8 +2,8 @@ process FUSIONINSPECTOR {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.12.0 bioconda::samtools=1.9 bioconda::star=2.7.8a"
-    container 'docker.io/trinityctat/starfusion:1.12.0'
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
+    container 'docker.io/trinityctat/starfusion:1.13.0'
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index e2bd7879f..a768cc5c9 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -1,8 +1,8 @@
 process STARFUSION_BUILD {
     tag 'star-fusion'
 
-    conda "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.12.0 bioconda::trinity=2.13.2 bioconda::samtools=1.9 bioconda::star=2.7.8a"
-    container "docker.io/trinityctat/starfusion:1.12.0"
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
+    container "docker.io/trinityctat/starfusion:1.13.0"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 1994d5551..9a46cc00d 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -2,8 +2,8 @@ process STARFUSION {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.12.0 bioconda::trinity=2.13.2 bioconda::samtools=1.9 bioconda::star=2.7.8a"
-    container 'docker.io/trinityctat/starfusion:1.12.0'
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
+    container 'docker.io/trinityctat/starfusion:1.13.0'
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 9c9b14827..221d12dd9 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,8 +1,8 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
-    conda "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.12.0 bioconda::trinity=2.13.2 bioconda::samtools=1.9 bioconda::star=2.7.8a"
-    container 'docker.io/trinityctat/starfusion:1.12.0'
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
+    container 'docker.io/trinityctat/starfusion:1.13.0'
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference

From 2c35b26ca16f88c5ad8ab79e2d5bf2c9442ec9c1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 12 Apr 2024 10:27:09 +0200
Subject: [PATCH 007/887] picard collectrnaseqmetrics to nf-core

---
 modules.json                                  |  14 +--
 .../local/picard/collectrnaseqmetrics/main.nf |  68 -----------
 .../picard/collectrnaseqmetrics/meta.yml      |  53 ---------
 modules/nf-core/agat/convertspgff2tsv/main.nf |  13 +-
 .../agat/convertspgff2tsv/tests/main.nf.test  |  61 ++++++++++
 .../convertspgff2tsv/tests/main.nf.test.snap  |  40 +++++++
 .../agat/convertspgff2tsv/tests/tags.yml      |   2 +
 modules/nf-core/arriba/arriba/main.nf         |   6 +-
 modules/nf-core/arriba/main.nf                |  66 -----------
 modules/nf-core/arriba/meta.yml               | 111 ------------------
 .../collectrnaseqmetrics/environment.yml      |   7 ++
 .../picard/collectrnaseqmetrics/main.nf       |  62 ++++++++++
 .../picard/collectrnaseqmetrics/meta.yml      |  61 ++++++++++
 subworkflows/local/qc_workflow.nf             |   5 +-
 workflows/rnafusion.nf                        |   1 -
 15 files changed, 258 insertions(+), 312 deletions(-)
 delete mode 100644 modules/local/picard/collectrnaseqmetrics/main.nf
 delete mode 100644 modules/local/picard/collectrnaseqmetrics/meta.yml
 create mode 100644 modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
 create mode 100644 modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/agat/convertspgff2tsv/tests/tags.yml
 delete mode 100644 modules/nf-core/arriba/main.nf
 delete mode 100644 modules/nf-core/arriba/meta.yml
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/environment.yml
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/main.nf
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/meta.yml

diff --git a/modules.json b/modules.json
index f14cf897a..1b751b9e8 100644
--- a/modules.json
+++ b/modules.json
@@ -7,17 +7,12 @@
                 "nf-core": {
                     "agat/convertspgff2tsv": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
-                    },
-                    "arriba": {
-                        "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "9198082dcec6d8374c0bf3d7e38dd1f79d4601a7",
                         "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "bbaeb3a3149def237b9d0acb9ab749ae98088e29",
                         "installed_by": ["modules"]
                     },
                     "arriba/download": {
@@ -70,6 +65,11 @@
                         "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
                         "installed_by": ["modules"]
                     },
+                    "picard/collectrnaseqmetrics": {
+                        "branch": "master",
+                        "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
+                        "installed_by": ["modules"]
+                    },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
diff --git a/modules/local/picard/collectrnaseqmetrics/main.nf b/modules/local/picard/collectrnaseqmetrics/main.nf
deleted file mode 100644
index ede593e0e..000000000
--- a/modules/local/picard/collectrnaseqmetrics/main.nf
+++ /dev/null
@@ -1,68 +0,0 @@
-process PICARD_COLLECTRNASEQMETRICS {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::picard=3.1.0"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.1.0--hdfd78af_0' :
-        'biocontainers/picard:3.1.0--hdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(bam), path(bai)
-    tuple val(meta2), path(refflat)
-    tuple val(meta3), path(rrna_intervals)
-
-    output:
-    tuple val(meta), path("*rna_metrics.txt")    , emit: metrics
-    path  "versions.yml"                        , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def strandedness = ''
-    // def strandedness = '--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND'
-    if ("${meta.strandedness}" == 'forward') {
-        strandedness = '--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND'
-    } else if ("${meta.strandedness}" == 'reverse') {
-        strandedness = '--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND'
-    } else {
-        strandedness = '--STRAND_SPECIFICITY NONE'
-    }
-
-    def rrna = rrna_intervals == [] ? '' : "--RIBOSOMAL_INTERVALS ${rrna_intervals}"
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def avail_mem = 3072
-    if (!task.memory) {
-        log.info '[Picard CollectRnaMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
-    }
-    """
-    picard \\
-        -Xmx${avail_mem}M \\
-        CollectRnaSeqMetrics \\
-        --TMP_DIR ./tmp \\
-        ${strandedness} \\
-        ${rrna} \\
-        --REF_FLAT ${refflat} \\
-        --INPUT ${bam} \\
-        --OUTPUT ${prefix}_rna_metrics.txt \\
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        picard: \$(picard CollectRnaMetrics --version 2>&1 | grep -o 'Version.*' | cut -f2- -d:)
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}_rna_metrics.txt
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        picard: \$(picard CollectRnaMetrics --version 2>&1 | grep -o 'Version.*' | cut -f2- -d:)
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/picard/collectrnaseqmetrics/meta.yml b/modules/local/picard/collectrnaseqmetrics/meta.yml
deleted file mode 100644
index 0cae7d07a..000000000
--- a/modules/local/picard/collectrnaseqmetrics/meta.yml
+++ /dev/null
@@ -1,53 +0,0 @@
-name: picard_collectrnaseqmetrics
-description: Produces RNA alignment metrics for a SAM or BAM file.
-keywords:
-  - alignment
-  - metrics
-  - statistics
-  - quality
-  - bam
-  - RNA
-tools:
-  - picard:
-      description: |
-        A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS)
-        data and formats such as SAM/BAM/CRAM and VCF.
-      homepage: https://broadinstitute.github.io/picard/
-      documentation: https://broadinstitute.github.io/picard/
-      licence: ["MIT"]
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM file
-      pattern: "*.{bam}"
-  - bai:
-      type: file
-      description: An optional BAM index file. If desired, --CREATE_INDEX must be passed as a flag
-      pattern: "*.{bai}"
-  - refflat:
-      type: file
-      description: Gene annotations in refFlat form
-  - rrna_intervals:
-      type: file
-      description: Location of rRNA sequences in genome, in interval_list format
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - metrics:
-      type: file
-      description: Alignment metrics files generated by picard
-      pattern: "*_{metrics}"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@rannick"
diff --git a/modules/nf-core/agat/convertspgff2tsv/main.nf b/modules/nf-core/agat/convertspgff2tsv/main.nf
index cef483600..5cded33ef 100644
--- a/modules/nf-core/agat/convertspgff2tsv/main.nf
+++ b/modules/nf-core/agat/convertspgff2tsv/main.nf
@@ -20,7 +20,6 @@ process AGAT_CONVERTSPGFF2TSV {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-
     """
     agat_convert_sp_gff2tsv.pl \\
         --gff $gff \\
@@ -32,4 +31,16 @@ process AGAT_CONVERTSPGFF2TSV {
         agat: \$(agat_convert_sp_gff2tsv.pl --help | sed '3!d; s/.*v//' | sed 's/ .*//')
     END_VERSIONS
     """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.tsv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        agat: \$(agat_convert_sp_gff2tsv.pl --help | sed '3!d; s/.*v//' | sed 's/ .*//')
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
new file mode 100644
index 000000000..41239a588
--- /dev/null
+++ b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
@@ -0,0 +1,61 @@
+nextflow_process {
+
+    name "Test Process AGAT_CONVERTSPGFF2TSV"
+    script "../main.nf"
+    process "AGAT_CONVERTSPGFF2TSV"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "agat"
+    tag "agat/convertspgff2tsv"
+
+    test("sarscov2 - genome [gff3]") {
+
+        when {
+            process {
+                """
+                // TODO nf-core: define inputs of the process here. Example:
+
+                input[0] = [
+                            [ id: 'test' ], // meta map
+                            file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - genome [gff3] - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id: 'test' ], // meta map
+                            file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.tsv.collect { file(it[1]).getName() } +
+                                process.out.versions).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
new file mode 100644
index 000000000..cf21cd659
--- /dev/null
+++ b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
@@ -0,0 +1,40 @@
+{
+    "sarscov2 - genome [gff3] - stub": {
+        "content": [
+            [
+                "test.tsv",
+                "versions.yml:md5,07ae8e1f648a67237a7efeafb551f01c"
+            ]
+        ],
+        "timestamp": "2023-12-24T23:55:30.786170144"
+    },
+    "sarscov2 - genome [gff3]": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.tsv:md5,e565b8ee5bc69b0625046a8036fda552"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,07ae8e1f648a67237a7efeafb551f01c"
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.tsv:md5,e565b8ee5bc69b0625046a8036fda552"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,07ae8e1f648a67237a7efeafb551f01c"
+                ]
+            }
+        ],
+        "timestamp": "2023-12-24T23:55:20.642366893"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/tags.yml b/modules/nf-core/agat/convertspgff2tsv/tests/tags.yml
new file mode 100644
index 000000000..d2ee37213
--- /dev/null
+++ b/modules/nf-core/agat/convertspgff2tsv/tests/tags.yml
@@ -0,0 +1,2 @@
+agat/convertspgff2tsv:
+  - "modules/nf-core/agat/convertspgff2tsv/**"
diff --git a/modules/nf-core/arriba/arriba/main.nf b/modules/nf-core/arriba/arriba/main.nf
index a29a8d140..761d0bf35 100644
--- a/modules/nf-core/arriba/arriba/main.nf
+++ b/modules/nf-core/arriba/arriba/main.nf
@@ -60,7 +60,9 @@ process ARRIBA_ARRIBA {
     echo stub > ${prefix}.fusions.tsv
     echo stub > ${prefix}.fusions.discarded.tsv
 
-    echo "${task.process}:" > versions.yml
-    echo ' arriba: 2.2.1' >> versions.yml
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        arriba: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
+    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/arriba/main.nf b/modules/nf-core/arriba/main.nf
deleted file mode 100644
index 2537d05b8..000000000
--- a/modules/nf-core/arriba/main.nf
+++ /dev/null
@@ -1,66 +0,0 @@
-process ARRIBA {
-    tag "$meta.id"
-    label 'process_medium'
-
-    conda "bioconda::arriba=2.4.0"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
-        'biocontainers/arriba:2.4.0--h0033a41_2' }"
-
-    input:
-    tuple val(meta), path(bam)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(gtf)
-    tuple val(meta4), path(blacklist)
-    tuple val(meta5), path(known_fusions)
-    tuple val(meta6), path(structural_variants)
-    tuple val(meta7), path(tags)
-    tuple val(meta8), path(protein_domains)
-
-    output:
-    tuple val(meta), path("*.fusions.tsv")          , emit: fusions
-    tuple val(meta), path("*.fusions.discarded.tsv"), emit: fusions_fail
-    path "versions.yml"                             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
-    def known_fusions = known_fusions ? "-k $known_fusions" : ""
-    def structural_variants = structural_variants ? "-d $structual_variants" : ""
-    def tags = tags ? "-t $tags" : ""
-    def protein_domains = protein_domains ? "-p $protein_domains" : ""
-
-    """
-    arriba \\
-        -x $bam \\
-        -a $fasta \\
-        -g $gtf \\
-        -o ${prefix}.fusions.tsv \\
-        -O ${prefix}.fusions.discarded.tsv \\
-        $blacklist \\
-        $known_fusions \\
-        $structural_variants \\
-        $tags \\
-        $protein_domains \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        arriba: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    echo stub > ${prefix}.fusions.tsv
-    echo stub > ${prefix}.fusions.discarded.tsv
-
-    echo "${task.process}:" > versions.yml
-    echo ' arriba: 2.2.1' >> versions.yml
-    """
-}
diff --git a/modules/nf-core/arriba/meta.yml b/modules/nf-core/arriba/meta.yml
deleted file mode 100644
index 85b3a30b5..000000000
--- a/modules/nf-core/arriba/meta.yml
+++ /dev/null
@@ -1,111 +0,0 @@
-name: arriba
-description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
-keywords:
-  - fusion
-  - arriba
-  - detection
-  - RNA-Seq
-tools:
-  - arriba:
-      description: Fast and accurate gene fusion detection from RNA-Seq data
-      homepage: https://github.com/suhrig/arriba
-      documentation: https://arriba.readthedocs.io/en/latest/
-      tool_dev_url: https://github.com/suhrig/arriba
-      doi: "10.1101/gr.257246.119"
-      licence: ["MIT"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fasta:
-      type: file
-      description: Assembly FASTA file
-      pattern: "*.{fasta}"
-  - meta3:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - gtf:
-      type: file
-      description: Annotation GTF file
-      pattern: "*.{gtf}"
-  - meta4:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - blacklist:
-      type: file
-      description: Blacklist file
-      pattern: "*.{tsv}"
-  - meta5:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - known_fusions:
-      type: file
-      description: Known fusions file
-      pattern: "*.{tsv}"
-  - meta6:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - structural_variants:
-      type: file
-      description: Structural variants file
-      pattern: "*.{tsv}"
-  - meta7:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - tags:
-      type: file
-      description: Tags file
-      pattern: "*.{tsv}"
-  - meta8:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - protein_domains:
-      type: file
-      description: Protein domains file
-      pattern: "*.{gff3}"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - fusions:
-      type: file
-      description: File contains fusions which pass all of Arriba's filters.
-      pattern: "*.{fusions.tsv}"
-  - fusions_fail:
-      type: file
-      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
-      pattern: "*.{fusions.discarded.tsv}"
-
-authors:
-  - "@praveenraj2018,@rannick"
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
new file mode 100644
index 000000000..542b2e823
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
@@ -0,0 +1,7 @@
+name: picard_collectrnaseqmetrics
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::picard=3.1.1
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/main.nf b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
new file mode 100644
index 000000000..f833a105c
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
@@ -0,0 +1,62 @@
+process PICARD_COLLECTRNASEQMETRICS {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' :
+        'biocontainers/picard:3.1.1--hdfd78af_0' }"
+
+    input:
+    tuple val(meta), path(bam)
+    path ref_flat
+    path fasta
+    path rrna_intervals
+
+    output:
+    tuple val(meta), path("*.rna_metrics")  , emit: metrics
+    tuple val(meta), path("*.pdf")          , emit: pdf, optional: true
+    path "versions.yml"                     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def reference = fasta ? "--REFERENCE_SEQUENCE ${fasta}" : ""
+    def rrna = rrna_intervals ? "--RIBOSOMAL_INTERVALS ${rrna_intervals}" : ""
+    def avail_mem = 3072
+    if (!task.memory) {
+        log.info '[Picard CollectRnaSeqMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
+    } else {
+        avail_mem = (task.memory.mega*0.8).intValue()
+    }
+    """
+    picard \\
+        -Xmx${avail_mem}M \\
+        CollectRnaSeqMetrics \\
+        $args \\
+        $reference \\
+        $rrna \\
+        --REF_FLAT $ref_flat \\
+        --INPUT $bam \\
+        --OUTPUT ${prefix}.rna_metrics
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        picard: \$(echo \$(picard CollectRnaSeqMetrics --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:)
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.rna_metrics
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        picard: \$(echo \$(picard CollectRnaSeqMetrics --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/meta.yml b/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
new file mode 100644
index 000000000..8a7bd2346
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
@@ -0,0 +1,61 @@
+name: "picard_collectrnaseqmetrics"
+description: Collect metrics from a RNAseq BAM file
+keywords:
+  - rna
+  - bam
+  - metrics
+  - alignment
+  - statistics
+  - quality
+tools:
+  - "picard":
+      description: |
+        A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS)
+        data and formats such as SAM/BAM/CRAM and VCF.
+      homepage: "https://broadinstitute.github.io/picard/"
+      documentation: "https://broadinstitute.github.io/picard/"
+      tool_dev_url: "https://github.com/broadinstitute/picard"
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false, strandedness:true ]
+  - bam:
+      type: file
+      description: BAM/SAM file
+      pattern: "*.{bam,sam}"
+  - ref_flat:
+      type: file
+      description: Genome ref_flat file
+  - gene_pred:
+      type: file
+      description: genome gene_pred file as an alternative to the ref_flat file
+  - fasta:
+      type: file
+      description: Genome fasta file
+  - rrna_intervals:
+      type: file
+      description: Interval file of ribosomal RNA regions
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - metrics:
+      type: file
+      description: RNA alignment metrics files generated by picard
+      pattern: "*.rna_metrics"
+  - pdf:
+      type: file
+      description: Plot normalized position vs. coverage in a pdf file generated by picard
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@anoronh4"
+maintainers:
+  - "@anoronh4"
diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index 6b53358ad..689314671 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -2,14 +2,13 @@
 // Check input samplesheet and get read channels
 //
 
-include { PICARD_COLLECTRNASEQMETRICS }                from '../../modules/local/picard/collectrnaseqmetrics/main'
+include { PICARD_COLLECTRNASEQMETRICS }                from '../../modules/nf-core/picard/collectrnaseqmetrics/main'
 include { GATK4_MARKDUPLICATES }                       from '../../modules/nf-core/gatk4/markduplicates/main'
 include { PICARD_COLLECTINSERTSIZEMETRICS }            from '../../modules/nf-core/picard/collectinsertsizemetrics/main'
 
 workflow QC_WORKFLOW {
     take:
         ch_bam_sorted
-        ch_bam_sorted_indexed
         ch_chrgtf
         ch_refflat
         ch_fasta
@@ -19,7 +18,7 @@ workflow QC_WORKFLOW {
     main:
         ch_versions = Channel.empty()
 
-        PICARD_COLLECTRNASEQMETRICS(ch_bam_sorted_indexed, ch_refflat, ch_rrna_interval)
+        PICARD_COLLECTRNASEQMETRICS(ch_bam_sorted, ch_refflat.map { meta, refflat -> [refflat]}, ch_rrna_interval.map { meta, rrna_interval -> [rrna_interval]})
         ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
         ch_rnaseq_metrics = Channel.empty().mix(PICARD_COLLECTRNASEQMETRICS.out.metrics)
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 7483f1be5..400df71aa 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -200,7 +200,6 @@ workflow RNAFUSION {
     //QC
     QC_WORKFLOW (
         STARFUSION_WORKFLOW.out.ch_bam_sorted,
-        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
         ch_chrgtf,
         ch_refflat,
         ch_fasta,

From 20b8b1b9bd46555f0117585d516965c1a2d1d184 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 12 Apr 2024 10:34:36 +0200
Subject: [PATCH 008/887] fix channels

---
 subworkflows/local/qc_workflow.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index 689314671..c13fd4940 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -18,7 +18,7 @@ workflow QC_WORKFLOW {
     main:
         ch_versions = Channel.empty()
 
-        PICARD_COLLECTRNASEQMETRICS(ch_bam_sorted, ch_refflat.map { meta, refflat -> [refflat]}, ch_rrna_interval.map { meta, rrna_interval -> [rrna_interval]})
+        PICARD_COLLECTRNASEQMETRICS(ch_bam_sorted, ch_refflat.map { meta, refflat -> [refflat]},  ch_fasta.map { meta, fasta -> [fasta]}, ch_rrna_interval.map { meta, rrna_interval -> [rrna_interval]})
         ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
         ch_rnaseq_metrics = Channel.empty().mix(PICARD_COLLECTRNASEQMETRICS.out.metrics)
 

From ba4396b045140592dfa5cb01235a7bc751cbcc99 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 12 Apr 2024 11:05:49 +0200
Subject: [PATCH 009/887] use ucsc gtftogenepred from nf-core

---
 conf/modules.config                           | 17 +++---
 modules.json                                  |  5 ++
 .../local/uscs/custom_gtftogenepred/main.nf   | 37 -------------
 .../local/uscs/custom_gtftogenepred/meta.yml  | 34 ------------
 .../ucsc/gtftogenepred/environment.yml        |  7 +++
 modules/nf-core/ucsc/gtftogenepred/main.nf    | 54 +++++++++++++++++++
 modules/nf-core/ucsc/gtftogenepred/meta.yml   | 47 ++++++++++++++++
 workflows/build_references.nf                 |  6 +--
 workflows/rnafusion.nf                        |  2 +-
 9 files changed, 126 insertions(+), 83 deletions(-)
 delete mode 100644 modules/local/uscs/custom_gtftogenepred/main.nf
 delete mode 100644 modules/local/uscs/custom_gtftogenepred/meta.yml
 create mode 100644 modules/nf-core/ucsc/gtftogenepred/environment.yml
 create mode 100644 modules/nf-core/ucsc/gtftogenepred/main.nf
 create mode 100644 modules/nf-core/ucsc/gtftogenepred/meta.yml

diff --git a/conf/modules.config b/conf/modules.config
index dfb660787..850703297 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -122,14 +122,6 @@ process {
         ]
     }
 
-    withName: 'GTF_TO_REFFLAT' {
-        publishDir = [
-            path: { "${params.genomes_base}/ensembl" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-        ]
-    }
-
     withName: 'HGNC_DOWNLOAD' {
         publishDir = [
             path: { "${params.genomes_base}/hgnc" },
@@ -341,6 +333,15 @@ process {
         ]
     }
 
+    withName: 'UCSC_GTFTOGENEPRED' {
+        ext.args   = "-genePredExt -geneNameAsName2"
+        publishDir = [
+            path: { "${params.genomes_base}/ensembl" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
+
     withName: 'VCF_COLLECT' {
         ext.when = {!params.fusioninspector_only}
     }
diff --git a/modules.json b/modules.json
index 1b751b9e8..21254e699 100644
--- a/modules.json
+++ b/modules.json
@@ -114,6 +114,11 @@
                         "branch": "master",
                         "git_sha": "b1b959609bda44341120aed1766329909f54b8d0",
                         "installed_by": ["modules"]
+                    },
+                    "ucsc/gtftogenepred": {
+                        "branch": "master",
+                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "installed_by": ["modules"]
                     }
                 }
             },
diff --git a/modules/local/uscs/custom_gtftogenepred/main.nf b/modules/local/uscs/custom_gtftogenepred/main.nf
deleted file mode 100644
index 78fcbd296..000000000
--- a/modules/local/uscs/custom_gtftogenepred/main.nf
+++ /dev/null
@@ -1,37 +0,0 @@
-process GTF_TO_REFFLAT {
-    label 'process_low'
-
-    conda "bioconda::ucsc-gtftogenepred=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-gtftogenepred:377--ha8a8165_5' :
-        'quay.io/biocontainers/ucsc-gtftogenepred:377--ha8a8165_5' }"
-
-    input:
-    tuple val(meta), path (gtf)
-
-    output:
-    path('*.refflat'), emit: refflat
-
-    script:
-    def genepred = gtf + '.genepred'
-    def refflat = gtf + '.refflat'
-    """
-    gtfToGenePred -genePredExt -geneNameAsName2 ${gtf} ${genepred}
-    paste ${genepred} ${genepred} | cut -f12,16-25 > ${refflat}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gtfToGenePred: 377
-    END_VERSIONS
-    """
-
-    stub:
-    def refflat = gtf + '.refflat'
-    """
-    touch ${refflat}
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gtfToGenePred: 377
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/uscs/custom_gtftogenepred/meta.yml b/modules/local/uscs/custom_gtftogenepred/meta.yml
deleted file mode 100644
index 09711f438..000000000
--- a/modules/local/uscs/custom_gtftogenepred/meta.yml
+++ /dev/null
@@ -1,34 +0,0 @@
-name: gtf_to_refflat
-description: generate gene annotations in refFlat form
-  - gtftorefflat
-tools:
-  - gtf_to_refflat:
-      description: generate gene annotations in refFlat form
-      homepage: https://pachterlab.github.io/kallisto/
-      documentation: https://pachterlab.github.io/kallisto/manual
-      tool_dev_url: https://github.com/pachterlab/kallisto
-      doi: ""
-      licence: ["BSD-2-Clause"]
-
-input:
-  - fasta:
-      type: file
-      description: genome fasta file
-      pattern: "*.{fasta*}"
-  - reference:
-      type: directory
-      description: Path to kallisto index
-      pattern: "*"
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - fusions:
-      type: file
-      description: fusions
-      pattern: "*.txt"
-
-authors:
-  - "@rannick"
diff --git a/modules/nf-core/ucsc/gtftogenepred/environment.yml b/modules/nf-core/ucsc/gtftogenepred/environment.yml
new file mode 100644
index 000000000..5216fc8ff
--- /dev/null
+++ b/modules/nf-core/ucsc/gtftogenepred/environment.yml
@@ -0,0 +1,7 @@
+name: ucsc_gtftogenepred
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::ucsc-gtftogenepred=447
diff --git a/modules/nf-core/ucsc/gtftogenepred/main.nf b/modules/nf-core/ucsc/gtftogenepred/main.nf
new file mode 100644
index 000000000..88aace26b
--- /dev/null
+++ b/modules/nf-core/ucsc/gtftogenepred/main.nf
@@ -0,0 +1,54 @@
+process UCSC_GTFTOGENEPRED {
+    tag '${meta.id}'
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ucsc-gtftogenepred:447--h954228d_0':
+        'biocontainers/ucsc-gtftogenepred:447--h954228d_0' }"
+
+    input:
+    tuple val(meta), path(gtf)
+
+    output:
+    tuple val(meta), path("*.genepred"), emit: genepred
+    tuple val(meta), path("*.refflat") , emit: refflat , optional: true
+    path "versions.yml"                , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def gen_refflat = args.contains("-genePredExt") && args.contains("-geneNameAsName2") ? "true" : "false"
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '447' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    gtfToGenePred \\
+        $args \\
+        $gtf  \\
+        ${prefix}.genepred
+
+    if [ "${gen_refflat}" == "true" ] ; then
+        awk 'BEGIN { OFS="\\t"} {print \$12, \$1, \$2, \$3, \$4, \$5, \$6, \$7, \$8, \$9, \$10}' ${prefix}.genepred > ${prefix}.refflat
+    fi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '447'
+    """
+    touch ${prefix}.genepred
+    touch ${prefix}.refflat
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ucsc: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/ucsc/gtftogenepred/meta.yml b/modules/nf-core/ucsc/gtftogenepred/meta.yml
new file mode 100644
index 000000000..02122e410
--- /dev/null
+++ b/modules/nf-core/ucsc/gtftogenepred/meta.yml
@@ -0,0 +1,47 @@
+name: ucsc_gtftogenepred
+description: compute average score of bigwig over bed file
+keywords:
+  - gtf
+  - genepred
+  - refflat
+  - ucsc
+  - gtftogenepred
+tools:
+  - ucsc:
+      description: Convert GTF files to GenePred format
+      homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
+      licence: ["varies; see http://genome.ucsc.edu/license"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - gtf:
+      type: file
+      description: GTF file
+      pattern: "*.{gtf}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - genepred:
+      type: file
+      description: genepred file
+      pattern: "*.{genepred}"
+  - refflat:
+      type: file
+      description: refflat file
+      pattern: "*.{refflat}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@BarryDigby"
+  - "@anoronh4"
+maintainers:
+  - "@BarryDigby"
+  - "@anoronh4"
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index bd985e4fb..7e8c984ea 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -11,7 +11,7 @@ include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/
 include { HGNC_DOWNLOAD }                   from '../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../modules/local/starfusion/build/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
-include { GTF_TO_REFFLAT }                  from '../modules/local/uscs/custom_gtftogenepred/main'
+include { UCSC_GTFTOGENEPRED }              from '../modules/nf-core/ucsc/gtftogenepred/main'
 include { RRNA_TRANSCRIPTS }                from '../modules/local/rrnatranscripts/main'
 include { CONVERT2BED }                     from '../modules/local/convert2bed/main'
 /*
@@ -69,9 +69,9 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.starfusion_build){
-        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
+        UCSC_GTFTOGENEPRED(ENSEMBL_DOWNLOAD.out.chrgtf)
     } else {
-        GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
+        UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.chrgtf)
     }
 
     if (params.fusionreport || params.all) {
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 400df71aa..e9152c4ba 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -25,7 +25,7 @@ ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }
 ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
 ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
 ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
-ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
+// ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
 ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
 
 

From 91a2af2b4bced2a5762a4ef4f760e6f9832f1064 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Apr 2024 10:26:05 +0200
Subject: [PATCH 010/887] bedops convert2bed from nf-core

---
 modules.json                                  |  5 ++
 modules/local/convert2bed/main.nf             | 36 ----------
 modules/local/convert2bed/meta.yml            | 30 --------
 modules/local/rrnatranscripts/main.nf         |  7 +-
 .../bedops/convert2bed/environment.yml        |  7 ++
 modules/nf-core/bedops/convert2bed/main.nf    | 48 +++++++++++++
 modules/nf-core/bedops/convert2bed/meta.yml   | 48 +++++++++++++
 .../bedops/convert2bed/tests/main.nf.test     | 59 ++++++++++++++++
 .../convert2bed/tests/main.nf.test.snap       | 68 +++++++++++++++++++
 .../nf-core/bedops/convert2bed/tests/tags.yml |  2 +
 workflows/build_references.nf                 | 14 ++--
 11 files changed, 247 insertions(+), 77 deletions(-)
 delete mode 100644 modules/local/convert2bed/main.nf
 delete mode 100644 modules/local/convert2bed/meta.yml
 create mode 100644 modules/nf-core/bedops/convert2bed/environment.yml
 create mode 100644 modules/nf-core/bedops/convert2bed/main.nf
 create mode 100644 modules/nf-core/bedops/convert2bed/meta.yml
 create mode 100644 modules/nf-core/bedops/convert2bed/tests/main.nf.test
 create mode 100644 modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/bedops/convert2bed/tests/tags.yml

diff --git a/modules.json b/modules.json
index 21254e699..41cd9acd9 100644
--- a/modules.json
+++ b/modules.json
@@ -20,6 +20,11 @@
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
+                    "bedops/convert2bed": {
+                        "branch": "master",
+                        "git_sha": "bbd685cd308e4d8bcf0c2caf82da3ee3c9e24157",
+                        "installed_by": ["modules"]
+                    },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "9437e6053dccf4aafa022bfd6e7e9de67e625af8",
diff --git a/modules/local/convert2bed/main.nf b/modules/local/convert2bed/main.nf
deleted file mode 100644
index c4313f0a7..000000000
--- a/modules/local/convert2bed/main.nf
+++ /dev/null
@@ -1,36 +0,0 @@
-process CONVERT2BED {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "bioconda::bedops=2.4.41"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedops:2.4.41--h9f5acd7_0' :
-        'quay.io/biocontainers/bedops:2.4.41--h9f5acd7_0' }"
-
-    input:
-    tuple val(meta), path(gtf)
-
-    output:
-    tuple val(meta), path("*.bed") , emit: bed
-
-    script:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    convert2bed -i gtf < $gtf > ${prefix}.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        convert2bed: \$(convert2bed --version | grep vers | sed 's/^.*.version:  //')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.bed
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        convert2bed: \$(convert2bed --version | grep vers | sed 's/^.*.version:  //')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/convert2bed/meta.yml b/modules/local/convert2bed/meta.yml
deleted file mode 100644
index 31ff3649a..000000000
--- a/modules/local/convert2bed/meta.yml
+++ /dev/null
@@ -1,30 +0,0 @@
-name:
-description: convert from GTF to BED format
-  - convert2bed
-tools:
-  - convert2bed:
-      description: convert from GTF to BED format
-      homepage: https://github.com/bedops/bedops
-      documentation: https://bedops.readthedocs.io/en/latest/index.html
-      tool_dev_url: https://github.com/bedops/bedops
-      doi: ""
-      licence: ["GNU GENERAL PUBLIC LICENSE"]
-
-input:
-  - gtf:
-      type: file
-      description: Path to GTF file
-      pattern: "*.{gtf*}"
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - bed:
-      type: file
-      description: bed file
-      pattern: "*.bed"
-
-authors:
-  - "@rannick"
diff --git a/modules/local/rrnatranscripts/main.nf b/modules/local/rrnatranscripts/main.nf
index e4afc2845..c31de7076 100644
--- a/modules/local/rrnatranscripts/main.nf
+++ b/modules/local/rrnatranscripts/main.nf
@@ -2,12 +2,11 @@ process RRNA_TRANSCRIPTS {
     tag "$meta.id"
     label 'process_single'
 
-    conda "conda-forge::python=3.8.3"
+    conda "conda-forge::python=3.12.2"
 
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/python:3.9--1' :
-        'quay.io/biocontainers/python:3.9--1' }"
-
+        'https://depot.galaxyproject.org/singularity/python:3.12' :
+        'quay.io/biocontainers/python:3.12' }"
 
     input:
         tuple val(meta), path(gtf)
diff --git a/modules/nf-core/bedops/convert2bed/environment.yml b/modules/nf-core/bedops/convert2bed/environment.yml
new file mode 100644
index 000000000..049b29e9b
--- /dev/null
+++ b/modules/nf-core/bedops/convert2bed/environment.yml
@@ -0,0 +1,7 @@
+name: "bedops_convert2bed"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - "bioconda::bedops=2.4.41"
diff --git a/modules/nf-core/bedops/convert2bed/main.nf b/modules/nf-core/bedops/convert2bed/main.nf
new file mode 100644
index 000000000..a23efbd56
--- /dev/null
+++ b/modules/nf-core/bedops/convert2bed/main.nf
@@ -0,0 +1,48 @@
+process BEDOPS_CONVERT2BED {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bedops:2.4.41--h4ac6f70_2':
+        'biocontainers/bedops:2.4.41--h4ac6f70_2' }"
+
+    input:
+    tuple val(meta), path(in_file)
+
+    output:
+    tuple val(meta), path("*.bed"), emit: bed
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def format = in_file.getExtension()
+    """
+    convert2bed \\
+        $args \\
+        -i $format \\
+        < $in_file \\
+        > ${prefix}.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedops/convert2bed: \$(convert2bed --version | grep vers | sed 's/^.*.version:  //')
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bedops/convert2bed: \$(convert2bed --version | grep vers | sed 's/^.*.version:  //')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/bedops/convert2bed/meta.yml b/modules/nf-core/bedops/convert2bed/meta.yml
new file mode 100644
index 000000000..eea919650
--- /dev/null
+++ b/modules/nf-core/bedops/convert2bed/meta.yml
@@ -0,0 +1,48 @@
+name: "bedops_convert2bed"
+description: Convert BAM/GFF/GTF/GVF/PSL files to bed
+keywords:
+  - convert
+  - bed
+  - genomics
+tools:
+  - "bedops":
+      description: "High-performance genomic feature operations."
+      homepage: "https://bedops.readthedocs.io/en/latest/content/reference/file-management/conversion/convert2bed.html#convert2bed"
+      documentation: "https://bedops.readthedocs.io/en/latest/"
+      tool_dev_url: "https://github.com/bedops"
+      doi: "10.1093/bioinformatics/bts277"
+      licence: ["GNU v2"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1', single_end:false ]`
+
+  - input:
+      type: file
+      description: Sorted BAM/GFF/GTF/GVF/PSL file
+      pattern: "*.{bam,gff,gtf,gvf,psl}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. `[ id:'sample1', single_end:false ]`
+
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+
+  - bed:
+      type: file
+      description: Sorted BED file
+      pattern: "*.{bed}"
+
+authors:
+  - "@rannick"
+maintainers:
+  - "@rannick"
diff --git a/modules/nf-core/bedops/convert2bed/tests/main.nf.test b/modules/nf-core/bedops/convert2bed/tests/main.nf.test
new file mode 100644
index 000000000..183f8de6c
--- /dev/null
+++ b/modules/nf-core/bedops/convert2bed/tests/main.nf.test
@@ -0,0 +1,59 @@
+// nf-core modules test bedops/convert2bed
+nextflow_process {
+
+    name "Test Process BEDOPS_CONVERT2BED"
+    script "../main.nf"
+    process "BEDOPS_CONVERT2BED"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "bedops"
+    tag "bedops/convert2bed"
+
+    test("sarscov2 - gtf") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true)
+                    ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - gtf - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test' ], // meta map
+                    file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true)
+                    ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap b/modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap
new file mode 100644
index 000000000..e2482924d
--- /dev/null
+++ b/modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap
@@ -0,0 +1,68 @@
+{
+    "sarscov2 - gtf - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-11T12:06:14.755423333"
+    },
+    "sarscov2 - gtf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed:md5,adfdd36e848a62f4b0ea8a694abe9659"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.bed:md5,adfdd36e848a62f4b0ea8a694abe9659"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-11T12:06:08.876003152"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/bedops/convert2bed/tests/tags.yml b/modules/nf-core/bedops/convert2bed/tests/tags.yml
new file mode 100644
index 000000000..fd2c29935
--- /dev/null
+++ b/modules/nf-core/bedops/convert2bed/tests/tags.yml
@@ -0,0 +1,2 @@
+bedops/convert2bed:
+  - "modules/nf-core/bedops/convert2bed/**"
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 7e8c984ea..b1ea6d70c 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -4,26 +4,26 @@
 ========================================================================================
 */
 
-include { ARRIBA_DOWNLOAD }                 from '../modules/nf-core/arriba/download/main'
 include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../modules/local/starfusion/build/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
-include { UCSC_GTFTOGENEPRED }              from '../modules/nf-core/ucsc/gtftogenepred/main'
 include { RRNA_TRANSCRIPTS }                from '../modules/local/rrnatranscripts/main'
-include { CONVERT2BED }                     from '../modules/local/convert2bed/main'
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
 
+include { ARRIBA_DOWNLOAD }                 from '../modules/nf-core/arriba/download/main'
+include { BEDOPS_CONVERT2BED } from '../modules/nf-core/bedops/convert2bed/main'
+include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
+include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
 include { SAMTOOLS_FAIDX }                  from '../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genomegenerate/main'
-include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
-include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
+include { UCSC_GTFTOGENEPRED }              from '../modules/nf-core/ucsc/gtftogenepred/main'
 
 /*
 ========================================================================================
@@ -43,9 +43,9 @@ workflow BUILD_REFERENCES {
     GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.fasta)
 
     RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
-    CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
+    BEDOPS_CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
 
-    GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
+    GATK4_BEDTOINTERVALLIST(BEDOPS_CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
 
 
     if (params.starindex || params.all || params.starfusion || params.arriba) {

From b493b90162d65a55f232c759c3bbac8e64b76848 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Wed, 8 May 2024 14:01:06 +0000
Subject: [PATCH 011/887] Template update for nf-core/tools version 2.14.0

---
 .editorconfig                                 |   6 +-
 .github/PULL_REQUEST_TEMPLATE.md              |   2 +-
 .github/workflows/awsfulltest.yml             |  10 +-
 .github/workflows/awstest.yml                 |  12 +-
 .github/workflows/ci.yml                      |   4 +-
 .github/workflows/download_pipeline.yml       |  22 ++-
 .github/workflows/fix-linting.yml             |   6 +-
 .github/workflows/linting.yml                 |  18 +-
 .github/workflows/linting_comment.yml         |   2 +-
 .github/workflows/release-announcements.yml   |   6 +-
 .nf-core.yml                                  |   1 +
 .pre-commit-config.yaml                       |   3 +
 CHANGELOG.md                                  |   2 +-
 README.md                                     |   2 +-
 conf/base.config                              |   3 -
 conf/modules.config                           |   8 -
 conf/test.config                              |   2 +-
 conf/test_full.config                         |   2 +-
 docs/usage.md                                 |   2 +
 modules.json                                  |   4 +-
 modules/nf-core/fastqc/main.nf                |   6 +
 nextflow.config                               | 178 +++++++++---------
 nextflow_schema.json                          |   7 +
 pyproject.toml                                |  15 --
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  16 +-
 .../nf-core/utils_nfcore_pipeline/main.nf     |   8 +-
 workflows/rnafusion.nf                        |  46 +++--
 27 files changed, 222 insertions(+), 171 deletions(-)
 delete mode 100644 pyproject.toml

diff --git a/.editorconfig b/.editorconfig
index dd9ffa538..72dda289a 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -28,10 +28,6 @@ indent_style = unset
 [/assets/email*]
 indent_size = unset
 
-# ignore Readme
-[README.md]
-indent_style = unset
-
-# ignore python
+# ignore python and markdown
 [*.{py,md}]
 indent_style = unset
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index 8b85a0aae..ed1d027be 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
 - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core lint`).
-- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`).
+- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
 - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
 - [ ] Output Documentation in `docs/output.md` is updated.
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index a4b6b4d35..3c5e4c8ac 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -8,12 +8,12 @@ on:
     types: [published]
   workflow_dispatch:
 jobs:
-  run-tower:
+  run-platform:
     name: Run AWS full tests
     if: github.repository == 'nf-core/rnafusion'
     runs-on: ubuntu-latest
     steps:
-      - name: Launch workflow via tower
+      - name: Launch workflow via Seqera Platform
         uses: seqeralabs/action-tower-launch@v2
         # TODO nf-core: You can customise AWS full pipeline tests as required
         # Add full size test data (but still relatively small datasets for few samples)
@@ -33,7 +33,7 @@ jobs:
 
       - uses: actions/upload-artifact@v4
         with:
-          name: Tower debug log file
+          name: Seqera Platform debug log file
           path: |
-            tower_action_*.log
-            tower_action_*.json
+            seqera_platform_action_*.log
+            seqera_platform_action_*.json
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index cf29b8fdd..d3448f1be 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -5,13 +5,13 @@ name: nf-core AWS test
 on:
   workflow_dispatch:
 jobs:
-  run-tower:
+  run-platform:
     name: Run AWS tests
     if: github.repository == 'nf-core/rnafusion'
     runs-on: ubuntu-latest
     steps:
-      # Launch workflow using Tower CLI tool action
-      - name: Launch workflow via tower
+      # Launch workflow using Seqera Platform CLI tool action
+      - name: Launch workflow via Seqera Platform
         uses: seqeralabs/action-tower-launch@v2
         with:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
@@ -27,7 +27,7 @@ jobs:
 
       - uses: actions/upload-artifact@v4
         with:
-          name: Tower debug log file
+          name: Seqera Platform debug log file
           path: |
-            tower_action_*.log
-            tower_action_*.json
+            seqera_platform_action_*.log
+            seqera_platform_action_*.json
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 3390bf1ac..a776bde84 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -28,10 +28,10 @@ jobs:
           - "latest-everything"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
         with:
           version: "${{ matrix.NXF_VER }}"
 
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 08622fd51..2d20d6442 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -14,6 +14,8 @@ on:
   pull_request:
     types:
       - opened
+      - edited
+      - synchronize
     branches:
       - master
   pull_request_target:
@@ -28,11 +30,14 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - name: Disk space cleanup
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
       - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
         with:
@@ -65,8 +70,17 @@ jobs:
       - name: Inspect download
         run: tree ./${{ env.REPOTITLE_LOWERCASE }}
 
-      - name: Run the downloaded pipeline
+      - name: Run the downloaded pipeline (stub)
+        id: stub_run_pipeline
+        continue-on-error: true
         env:
           NXF_SINGULARITY_CACHEDIR: ./
           NXF_SINGULARITY_HOME_MOUNT: true
         run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
+      - name: Run the downloaded pipeline (stub run not supported)
+        id: run_pipeline
+        if: ${{ job.steps.stub_run_pipeline.status == failure() }}
+        env:
+          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_HOME_MOUNT: true
+        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index 36ecd2076..df750880c 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
@@ -32,9 +32,9 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
       # Install and run pre-commit
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
+          python-version: "3.12"
 
       - name: Install pre-commit
         run: pip install pre-commit
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 073e18767..a3fb25414 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,12 +14,12 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
-      - name: Set up Python 3.11
-        uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - name: Set up Python 3.12
+        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: 3.11
+          python-version: "3.12"
           cache: "pip"
 
       - name: Install pre-commit
@@ -32,14 +32,14 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v1
+        uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
-          python-version: "3.11"
+          python-version: "3.12"
           architecture: "x64"
 
       - name: Install dependencies
@@ -60,7 +60,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
+        uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index b706875fc..40acc23f5 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3
+        uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index d468aeaae..03ecfcf72 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -12,7 +12,7 @@ jobs:
       - name: get topics and convert to hashtags
         id: get_topics
         run: |
-          curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT
+          echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')"  >> $GITHUB_OUTPUT
 
       - uses: rzr/fediverse-action@master
         with:
@@ -25,13 +25,13 @@ jobs:
 
             Please see the changelog: ${{ github.event.release.html_url }}
 
-            ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics
+            ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
 
   send-tweet:
     runs-on: ubuntu-latest
 
     steps:
-      - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
+      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
           python-version: "3.10"
       - name: Install dependencies
diff --git a/.nf-core.yml b/.nf-core.yml
index 3805dc81c..d6daa4034 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1 +1,2 @@
 repository_type: pipeline
+nf_core_version: "2.14.0"
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index af57081f6..4dc0f1dcd 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -3,6 +3,9 @@ repos:
     rev: "v3.1.0"
     hooks:
       - id: prettier
+        additional_dependencies:
+          - prettier@3.2.5
+
   - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
     rev: "2.7.3"
     hooks:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index a01e6117e..9b6d5e6a8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v3.0.2dev - [date]
+## v4.0.0dev - [date]
 
 Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template.
 
diff --git a/README.md b/README.md
index 805de70e0..5075b862d 100644
--- a/README.md
+++ b/README.md
@@ -13,7 +13,7 @@
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
-[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/rnafusion)
+[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)
 
 [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
 
diff --git a/conf/base.config b/conf/base.config
index 51042a029..0af2fc0da 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -59,7 +59,4 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
-    withName:CUSTOM_DUMPSOFTWAREVERSIONS {
-        cache = false
-    }
 }
diff --git a/conf/modules.config b/conf/modules.config
index e3ea8fa6c..d203d2b6e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -22,14 +22,6 @@ process {
         ext.args = '--quiet'
     }
 
-    withName: CUSTOM_DUMPSOFTWAREVERSIONS {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            pattern: '*_versions.yml'
-        ]
-    }
-
     withName: 'MULTIQC' {
         ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
diff --git a/conf/test.config b/conf/test.config
index 796db260d..d54c7159b 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -22,7 +22,7 @@ params {
     // Input data
     // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
     // TODO nf-core: Give any required params for the test so that command line flags are not needed
-    input  = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
+    input  = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
 
     // Genome references
     genome = 'R64-1-1'
diff --git a/conf/test_full.config b/conf/test_full.config
index e2522d720..7662ac58d 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -17,7 +17,7 @@ params {
     // Input data for full size test
     // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA)
     // TODO nf-core: Give any required params for the test so that command line flags are not needed
-    input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
+    input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv'
 
     // Genome references
     genome = 'R64-1-1'
diff --git a/docs/usage.md b/docs/usage.md
index a39d7dc4b..612cb410f 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -156,6 +156,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
   - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
 - `apptainer`
   - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
+- `wave`
+  - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later).
 - `conda`
   - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
 
diff --git a/modules.json b/modules.json
index 5002e3469..c253f7934 100644
--- a/modules.json
+++ b/modules.json
@@ -7,7 +7,7 @@
                 "nf-core": {
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
+                        "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
@@ -26,7 +26,7 @@
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfvalidation_plugin": {
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 9e19a74c5..d79f1c862 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -25,6 +25,11 @@ process FASTQC {
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
     def rename_to = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
+
+    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB')
+    // FastQC memory value allowed range (100 - 10000)
+    def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
+
     """
     printf "%s %s\\n" $rename_to | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
@@ -33,6 +38,7 @@ process FASTQC {
     fastqc \\
         $args \\
         --threads $task.cpus \\
+        --memory $fastqc_memory \\
         $renamed_files
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/nextflow.config b/nextflow.config
index fc563542d..172afb674 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,7 +16,8 @@ params {
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'
     igenomes_ignore            = false
-    fasta                      = null// MultiQC options
+
+    // MultiQC options
     multiqc_config             = null
     multiqc_title              = null
     multiqc_logo               = null
@@ -24,15 +25,16 @@ params {
     multiqc_methods_description = null
 
     // Boilerplate options
-    outdir                     = null
-    publish_dir_mode           = 'copy'
-    email                      = null
-    email_on_fail              = null
-    plaintext_email            = false
-    monochrome_logs            = false
-    hook_url                   = null
-    help                       = false
-    version                    = false
+    outdir                       = null
+    publish_dir_mode             = 'copy'
+    email                        = null
+    email_on_fail                = null
+    plaintext_email              = false
+    monochrome_logs              = false
+    hook_url                     = null
+    help                         = false
+    version                      = false
+    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/'
 
     // Config options
     config_profile_name        = null
@@ -68,103 +70,109 @@ try {
 }
 
 // Load nf-core/rnafusion custom profiles from different institutions.
-// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs!
-// try {
-//   includeConfig "${params.custom_config_base}/pipeline/rnafusion.config"
-// } catch (Exception e) {
-//   System.err.println("WARNING: Could not load nf-core/config/rnafusion profiles: ${params.custom_config_base}/pipeline/rnafusion.config")
-// }
+try {
+    includeConfig "${params.custom_config_base}/pipeline/rnafusion.config"
+} catch (Exception e) {
+    System.err.println("WARNING: Could not load nf-core/config/rnafusion profiles: ${params.custom_config_base}/pipeline/rnafusion.config")
+}
 profiles {
     debug {
-        dumpHashes             = true
-        process.beforeScript   = 'echo $HOSTNAME'
-        cleanup                = false
+        dumpHashes              = true
+        process.beforeScript    = 'echo $HOSTNAME'
+        cleanup                 = false
         nextflow.enable.configProcessNamesValidation = true
     }
     conda {
-        conda.enabled          = true
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        channels               = ['conda-forge', 'bioconda', 'defaults']
-        apptainer.enabled      = false
+        conda.enabled           = true
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        conda.channels          = ['conda-forge', 'bioconda', 'defaults']
+        apptainer.enabled       = false
     }
     mamba {
-        conda.enabled          = true
-        conda.useMamba         = true
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        conda.enabled           = true
+        conda.useMamba          = true
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     docker {
-        docker.enabled         = true
-        conda.enabled          = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
-        docker.runOptions      = '-u $(id -u):$(id -g)'
+        docker.enabled          = true
+        conda.enabled           = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
+        docker.runOptions       = '-u $(id -u):$(id -g)'
     }
     arm {
-        docker.runOptions      = '-u $(id -u):$(id -g) --platform=linux/amd64'
+        docker.runOptions       = '-u $(id -u):$(id -g) --platform=linux/amd64'
     }
     singularity {
-        singularity.enabled    = true
-        singularity.autoMounts = true
-        conda.enabled          = false
-        docker.enabled         = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        singularity.enabled     = true
+        singularity.autoMounts  = true
+        conda.enabled           = false
+        docker.enabled          = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     podman {
-        podman.enabled         = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        podman.enabled          = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     shifter {
-        shifter.enabled        = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        charliecloud.enabled   = false
-        apptainer.enabled      = false
+        shifter.enabled         = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        charliecloud.enabled    = false
+        apptainer.enabled       = false
     }
     charliecloud {
-        charliecloud.enabled   = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        apptainer.enabled      = false
+        charliecloud.enabled    = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        apptainer.enabled       = false
     }
     apptainer {
-        apptainer.enabled      = true
-        apptainer.autoMounts   = true
-        conda.enabled          = false
-        docker.enabled         = false
-        singularity.enabled    = false
-        podman.enabled         = false
-        shifter.enabled        = false
-        charliecloud.enabled   = false
+        apptainer.enabled       = true
+        apptainer.autoMounts    = true
+        conda.enabled           = false
+        docker.enabled          = false
+        singularity.enabled     = false
+        podman.enabled          = false
+        shifter.enabled         = false
+        charliecloud.enabled    = false
+    }
+    wave {
+        apptainer.ociAutoPull   = true
+        singularity.ociAutoPull = true
+        wave.enabled            = true
+        wave.freeze             = true
+        wave.strategy           = 'conda,container'
     }
     gitpod {
-        executor.name          = 'local'
-        executor.cpus          = 4
-        executor.memory        = 8.GB
+        executor.name           = 'local'
+        executor.cpus           = 4
+        executor.memory         = 8.GB
     }
     test      { includeConfig 'conf/test.config'      }
     test_full { includeConfig 'conf/test_full.config' }
@@ -231,7 +239,7 @@ manifest {
     description     = """Nextflow rnafusion analysis pipeline, part of the nf-core community."""
     mainScript      = 'main.nf'
     nextflowVersion = '!>=23.04.0'
-    version         = '3.0.2dev'
+    version         = '4.0.0dev'
     doi             = ''
 }
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 025bd65df..91bbdef8c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -265,6 +265,13 @@
                     "description": "Validation of parameters in lenient more.",
                     "hidden": true,
                     "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
+                },
+                "pipelines_testdata_base_path": {
+                    "type": "string",
+                    "fa_icon": "far fa-check-circle",
+                    "description": "Base URL or local path to location of pipeline test dataset files",
+                    "default": "https://raw.githubusercontent.com/nf-core/test-datasets/",
+                    "hidden": true
                 }
             }
         }
diff --git a/pyproject.toml b/pyproject.toml
deleted file mode 100644
index 56110621e..000000000
--- a/pyproject.toml
+++ /dev/null
@@ -1,15 +0,0 @@
-# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff.
-# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation.
-[tool.ruff]
-line-length = 120
-target-version = "py38"
-cache-dir = "~/.cache/ruff"
-
-[tool.ruff.lint]
-select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"]
-
-[tool.ruff.lint.isort]
-known-first-party = ["nf_core"]
-
-[tool.ruff.lint.per-file-ignores]
-"__init__.py" = ["E402", "F401"]
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index c24a3741a..8d187230d 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -140,6 +140,10 @@ workflow PIPELINE_COMPLETION {
             imNotification(summary_params, hook_url)
         }
     }
+
+    workflow.onError {
+        log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting"
+    }
 }
 
 /*
@@ -230,8 +234,16 @@ def methodsDescriptionText(mqc_methods_yaml) {
     meta["manifest_map"] = workflow.manifest.toMap()
 
     // Pipeline DOI
-    meta["doi_text"] = meta.manifest_map.doi ? "(doi: <a href=\'https://doi.org/${meta.manifest_map.doi}\'>${meta.manifest_map.doi}</a>)" : ""
-    meta["nodoi_text"] = meta.manifest_map.doi ? "": "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
+    if (meta.manifest_map.doi) {
+        // Using a loop to handle multiple DOIs
+        // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
+        // Removing ` ` since the manifest.doi is a string and not a proper list
+        def temp_doi_ref = ""
+        String[] manifest_doi = meta.manifest_map.doi.tokenize(",")
+        for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: <a href=\'https://doi.org/${doi_ref.replace("https://doi.org/", "").replace(" ", "")}\'>${doi_ref.replace("https://doi.org/", "").replace(" ", "")}</a>), "
+        meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2)
+    } else meta["doi_text"] = ""
+    meta["nodoi_text"] = meta.manifest_map.doi ? "" : "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
 
     // Tool references
     meta["tool_citations"] = ""
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index a8b55d6fe..14558c392 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) {
 // Citation string for pipeline
 //
 def workflowCitation() {
+    def temp_doi_ref = ""
+    String[] manifest_doi = workflow.manifest.doi.tokenize(",")
+    // Using a loop to handle multiple DOIs
+    // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
+    // Removing ` ` since the manifest.doi is a string and not a proper list
+    for (String doi_ref: manifest_doi) temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
     return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
         "* The pipeline\n" +
-        "  ${workflow.manifest.doi}\n\n" +
+        temp_doi_ref + "\n" +
         "* The nf-core framework\n" +
         "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
         "* Software dependencies\n" +
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 62c2cf15c..2b01b6da0 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -40,22 +40,44 @@ workflow RNAFUSION {
     // Collate and save software versions
     //
     softwareVersionsToYAML(ch_versions)
-        .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true)
-        .set { ch_collated_versions }
+        .collectFile(
+            storeDir: "${params.outdir}/pipeline_info",
+            name: 'nf_core_pipeline_software_mqc_versions.yml',
+            sort: true,
+            newLine: true
+        ).set { ch_collated_versions }
 
     //
     // MODULE: MultiQC
     //
-    ch_multiqc_config                     = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-    ch_multiqc_custom_config              = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty()
-    ch_multiqc_logo                       = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty()
-    summary_params                        = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary                   = Channel.value(paramsSummaryMultiqc(summary_params))
-    ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description))
-    ch_multiqc_files                      = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files                      = ch_multiqc_files.mix(ch_collated_versions)
-    ch_multiqc_files                      = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false))
+    ch_multiqc_config        = Channel.fromPath(
+        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+    ch_multiqc_custom_config = params.multiqc_config ?
+        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+        Channel.empty()
+    ch_multiqc_logo          = params.multiqc_logo ?
+        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+        Channel.empty()
+
+    summary_params      = paramsSummaryMap(
+        workflow, parameters_schema: "nextflow_schema.json")
+    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+
+    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
+        file(params.multiqc_methods_description, checkIfExists: true) :
+        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+    ch_methods_description                = Channel.value(
+        methodsDescriptionText(ch_multiqc_custom_methods_description))
+
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_methods_description.collectFile(
+            name: 'methods_description_mqc.yaml',
+            sort: true
+        )
+    )
 
     MULTIQC (
         ch_multiqc_files.collect(),

From aeca9e2ebe9f9ec7eb0dd84b3907eaf7bf58cb6a Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 9 May 2024 11:43:56 +0000
Subject: [PATCH 012/887] Template update for nf-core/tools version 2.14.1

---
 .github/workflows/linting.yml | 1 -
 .nf-core.yml                  | 2 +-
 2 files changed, 1 insertion(+), 2 deletions(-)

diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index a3fb25414..1fcafe880 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -20,7 +20,6 @@ jobs:
         uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
         with:
           python-version: "3.12"
-          cache: "pip"
 
       - name: Install pre-commit
         run: pip install pre-commit
diff --git a/.nf-core.yml b/.nf-core.yml
index d6daa4034..e0b85a77f 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,2 +1,2 @@
 repository_type: pipeline
-nf_core_version: "2.14.0"
+nf_core_version: "2.14.1"

From 2da759096716c8d2a9e46d200dd5c9ba697c126c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 14 May 2024 10:59:56 +0200
Subject: [PATCH 013/887] add salmon first steps

---
 modules.json                                  |  10 ++
 modules/nf-core/salmon/index/environment.yml  |   7 +
 modules/nf-core/salmon/index/main.nf          |  47 +++++
 modules/nf-core/salmon/index/meta.yml         |  37 ++++
 .../nf-core/salmon/index/tests/main.nf.test   |  35 ++++
 .../salmon/index/tests/main.nf.test.snap      |  14 ++
 modules/nf-core/salmon/index/tests/tags.yml   |   2 +
 modules/nf-core/salmon/quant/environment.yml  |   7 +
 modules/nf-core/salmon/quant/main.nf          |  79 +++++++++
 modules/nf-core/salmon/quant/meta.yml         |  62 +++++++
 .../nf-core/salmon/quant/tests/main.nf.test   | 160 ++++++++++++++++++
 .../salmon/quant/tests/main.nf.test.snap      |  50 ++++++
 .../salmon/quant/tests/nextflow.config        |   7 +
 modules/nf-core/salmon/quant/tests/tags.yml   |   2 +
 subworkflows/local/qc_workflow.nf             |   5 +-
 workflows/build_references.nf                 |   2 +
 workflows/rnafusion.nf                        |   1 +
 17 files changed, 526 insertions(+), 1 deletion(-)
 create mode 100644 modules/nf-core/salmon/index/environment.yml
 create mode 100644 modules/nf-core/salmon/index/main.nf
 create mode 100644 modules/nf-core/salmon/index/meta.yml
 create mode 100644 modules/nf-core/salmon/index/tests/main.nf.test
 create mode 100644 modules/nf-core/salmon/index/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/salmon/index/tests/tags.yml
 create mode 100644 modules/nf-core/salmon/quant/environment.yml
 create mode 100644 modules/nf-core/salmon/quant/main.nf
 create mode 100644 modules/nf-core/salmon/quant/meta.yml
 create mode 100644 modules/nf-core/salmon/quant/tests/main.nf.test
 create mode 100644 modules/nf-core/salmon/quant/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/salmon/quant/tests/nextflow.config
 create mode 100644 modules/nf-core/salmon/quant/tests/tags.yml

diff --git a/modules.json b/modules.json
index 23a6ce29f..384c60a53 100644
--- a/modules.json
+++ b/modules.json
@@ -65,6 +65,16 @@
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
+                    "salmon/index": {
+                        "branch": "master",
+                        "git_sha": "ffc101e1b84ef3df2e4e4a966e84b3c513ae5693",
+                        "installed_by": ["modules"]
+                    },
+                    "salmon/quant": {
+                        "branch": "master",
+                        "git_sha": "03a8562231d575c313266c193a980594b941e3ea",
+                        "installed_by": ["modules"]
+                    },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
diff --git a/modules/nf-core/salmon/index/environment.yml b/modules/nf-core/salmon/index/environment.yml
new file mode 100644
index 000000000..a6607d964
--- /dev/null
+++ b/modules/nf-core/salmon/index/environment.yml
@@ -0,0 +1,7 @@
+name: salmon_index
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::salmon=1.10.1
diff --git a/modules/nf-core/salmon/index/main.nf b/modules/nf-core/salmon/index/main.nf
new file mode 100644
index 000000000..88d9cf14a
--- /dev/null
+++ b/modules/nf-core/salmon/index/main.nf
@@ -0,0 +1,47 @@
+process SALMON_INDEX {
+    tag "$transcript_fasta"
+    label "process_medium"
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/salmon:1.10.1--h7e5ed60_0' :
+        'biocontainers/salmon:1.10.1--h7e5ed60_0' }"
+
+    input:
+    path genome_fasta
+    path transcript_fasta
+
+    output:
+    path "salmon"      , emit: index
+    path "versions.yml", emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def get_decoy_ids = "grep '^>' $genome_fasta | cut -d ' ' -f 1 | cut -d \$'\\t' -f 1 > decoys.txt"
+    def gentrome      = "gentrome.fa"
+    if (genome_fasta.endsWith('.gz')) {
+        get_decoy_ids = "grep '^>' <(gunzip -c $genome_fasta) | cut -d ' ' -f 1 | cut -d \$'\\t' -f 1 > decoys.txt"
+        gentrome      = "gentrome.fa.gz"
+    }
+    """
+    $get_decoy_ids
+    sed -i.bak -e 's/>//g' decoys.txt
+    cat $transcript_fasta $genome_fasta > $gentrome
+
+    salmon \\
+        index \\
+        --threads $task.cpus \\
+        -t $gentrome \\
+        -d decoys.txt \\
+        $args \\
+        -i salmon
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/salmon/index/meta.yml b/modules/nf-core/salmon/index/meta.yml
new file mode 100644
index 000000000..fd94dd274
--- /dev/null
+++ b/modules/nf-core/salmon/index/meta.yml
@@ -0,0 +1,37 @@
+name: salmon_index
+description: Create index for salmon
+keywords:
+  - index
+  - fasta
+  - genome
+  - reference
+tools:
+  - salmon:
+      description: |
+        Salmon is a tool for wicked-fast transcript quantification from RNA-seq data
+      homepage: https://salmon.readthedocs.io/en/latest/salmon.html
+      manual: https://salmon.readthedocs.io/en/latest/salmon.html
+      doi: 10.1038/nmeth.4197
+      licence: ["GPL-3.0-or-later"]
+input:
+  - genome_fasta:
+      type: file
+      description: Fasta file of the reference genome
+  - transcriptome_fasta:
+      type: file
+      description: Fasta file of the reference transcriptome
+output:
+  - index:
+      type: directory
+      description: Folder containing the star index files
+      pattern: "salmon"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"
+maintainers:
+  - "@kevinmenden"
+  - "@drpatelh"
diff --git a/modules/nf-core/salmon/index/tests/main.nf.test b/modules/nf-core/salmon/index/tests/main.nf.test
new file mode 100644
index 000000000..538b231b4
--- /dev/null
+++ b/modules/nf-core/salmon/index/tests/main.nf.test
@@ -0,0 +1,35 @@
+nextflow_process {
+
+    name "Test Process SALMON_INDEX"
+    script "../main.nf"
+    process "SALMON_INDEX"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "salmon"
+    tag "salmon/index"
+
+    test("sarscov2") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.fasta", checkIfExists: true)])
+                input[1] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.index.get(0)).exists() },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/salmon/index/tests/main.nf.test.snap b/modules/nf-core/salmon/index/tests/main.nf.test.snap
new file mode 100644
index 000000000..94edf3903
--- /dev/null
+++ b/modules/nf-core/salmon/index/tests/main.nf.test.snap
@@ -0,0 +1,14 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,563eeafb4577be0b13801d7021c0bf42"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2023-11-22T14:26:33.32036"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/salmon/index/tests/tags.yml b/modules/nf-core/salmon/index/tests/tags.yml
new file mode 100644
index 000000000..02997890f
--- /dev/null
+++ b/modules/nf-core/salmon/index/tests/tags.yml
@@ -0,0 +1,2 @@
+salmon/index:
+  - modules/nf-core/salmon/index/**
diff --git a/modules/nf-core/salmon/quant/environment.yml b/modules/nf-core/salmon/quant/environment.yml
new file mode 100644
index 000000000..862667241
--- /dev/null
+++ b/modules/nf-core/salmon/quant/environment.yml
@@ -0,0 +1,7 @@
+name: salmon_quant
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::salmon=1.10.1
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
new file mode 100644
index 000000000..e115d39cf
--- /dev/null
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -0,0 +1,79 @@
+process SALMON_QUANT {
+    tag "$meta.id"
+    label "process_medium"
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/salmon:1.10.1--h7e5ed60_0' :
+        'biocontainers/salmon:1.10.1--h7e5ed60_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+    path  index
+    path  gtf
+    path  transcript_fasta
+    val   alignment_mode
+    val   lib_type
+
+    output:
+    tuple val(meta), path("${prefix}") , emit: results
+    tuple val(meta), path("*info.json"), emit: json_info, optional: true
+    path  "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args   ?: ''
+    prefix   = task.ext.prefix ?: "${meta.id}"
+
+    def reference   = "--index $index"
+    def reads1 = [], reads2 = []
+    meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
+    def input_reads = meta.single_end ? "-r ${reads1.join(" ")}" : "-1 ${reads1.join(" ")} -2 ${reads2.join(" ")}"
+    if (alignment_mode) {
+        reference   = "-t $transcript_fasta"
+        input_reads = "-a $reads"
+    }
+
+    def strandedness_opts = [
+        'A', 'U', 'SF', 'SR',
+        'IS', 'IU' , 'ISF', 'ISR',
+        'OS', 'OU' , 'OSF', 'OSR',
+        'MS', 'MU' , 'MSF', 'MSR'
+    ]
+    def strandedness =  'A'
+    if (lib_type) {
+        if (strandedness_opts.contains(lib_type)) {
+            strandedness = lib_type
+        } else {
+            log.info "[Salmon Quant] Invalid library type specified '--libType=${lib_type}', defaulting to auto-detection with '--libType=A'."
+        }
+    } else {
+        strandedness = meta.single_end ? 'U' : 'IU'
+        if (meta.strandedness == 'forward') {
+            strandedness = meta.single_end ? 'SF' : 'ISF'
+        } else if (meta.strandedness == 'reverse') {
+            strandedness = meta.single_end ? 'SR' : 'ISR'
+        }
+    }
+    """
+    salmon quant \\
+        --geneMap $gtf \\
+        --threads $task.cpus \\
+        --libType=$strandedness \\
+        $reference \\
+        $input_reads \\
+        $args \\
+        -o $prefix
+
+    if [ -f $prefix/aux_info/meta_info.json ]; then
+        cp $prefix/aux_info/meta_info.json "${prefix}_meta_info.json"
+    fi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/salmon/quant/meta.yml b/modules/nf-core/salmon/quant/meta.yml
new file mode 100644
index 000000000..a2faf589a
--- /dev/null
+++ b/modules/nf-core/salmon/quant/meta.yml
@@ -0,0 +1,62 @@
+name: salmon_quant
+description: gene/transcript quantification with Salmon
+keywords:
+  - index
+  - fasta
+  - genome
+  - reference
+tools:
+  - salmon:
+      description: |
+        Salmon is a tool for wicked-fast transcript quantification from RNA-seq data
+      homepage: https://salmon.readthedocs.io/en/latest/salmon.html
+      manual: https://salmon.readthedocs.io/en/latest/salmon.html
+      doi: 10.1038/nmeth.4197
+      licence: ["GPL-3.0-or-later"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files for single-end or paired-end data.
+        Multiple single-end fastqs or pairs of paired-end fastqs are
+        handled.
+  - index:
+      type: directory
+      description: Folder containing the star index files
+  - gtf:
+      type: file
+      description: GTF of the reference transcriptome
+  - transcript_fasta:
+      type: file
+      description: Fasta file of the reference transcriptome
+  - alignment_mode:
+      type: boolean
+      description: whether to run salmon in alignment mode
+  - lib_type:
+      type: string
+      description: |
+        Override library type inferred based on strandedness defined in meta object
+output:
+  - results:
+      type: directory
+      description: Folder containing the quantification results for a specific sample
+      pattern: "${prefix}"
+  - json_info:
+      type: file
+      description: File containing meta information from Salmon quant
+      pattern: "*info.json"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@kevinmenden"
+  - "@drpatelh"
+maintainers:
+  - "@kevinmenden"
+  - "@drpatelh"
diff --git a/modules/nf-core/salmon/quant/tests/main.nf.test b/modules/nf-core/salmon/quant/tests/main.nf.test
new file mode 100644
index 000000000..04e61e2b3
--- /dev/null
+++ b/modules/nf-core/salmon/quant/tests/main.nf.test
@@ -0,0 +1,160 @@
+nextflow_process {
+
+    name "Test Process SALMON_QUANT"
+    script "../main.nf"
+    process "SALMON_QUANT"
+    config "./nextflow.config"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "salmon"
+    tag "salmon/quant"
+    tag "salmon/index"
+
+    setup {
+        run("SALMON_INDEX") {
+            script "../../../salmon/index/main.nf"
+            process {
+                """
+                input[0] = Channel.of([file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.fasta", checkIfExists: true)])
+                input[1] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                """
+            }
+        }
+    }
+
+    test("sarscov2 - single_end") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: true ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = ''
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_single_end") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - single_end lib type A") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: true ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = 'A'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_single_end_lib_type_a") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - pair_end") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: true ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
+                    ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = ''
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_pair_end") }
+            )
+        }
+
+    }
+
+    test("sarscov2 - pair_end multiple") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test2_1.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test2_2.fastq.gz", checkIfExists: true)
+                    ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = ''
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_pair_end_multiple") }
+            )
+        }
+
+    }
+}
diff --git a/modules/nf-core/salmon/quant/tests/main.nf.test.snap b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
new file mode 100644
index 000000000..a1ec792e0
--- /dev/null
+++ b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
@@ -0,0 +1,50 @@
+{
+    "versions_single_end": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-01T11:51:01.865730832"
+    },
+    "versions_pair_end": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-06T17:10:56.121713"
+    },
+    "versions_single_end_lib_type_a": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-06T17:09:14.898351"
+    },
+    "versions_pair_end_multiple": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-06T17:11:07.846643"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/salmon/quant/tests/nextflow.config b/modules/nf-core/salmon/quant/tests/nextflow.config
new file mode 100644
index 000000000..37c082129
--- /dev/null
+++ b/modules/nf-core/salmon/quant/tests/nextflow.config
@@ -0,0 +1,7 @@
+process {
+
+    withName: SALMON_QUANT {
+        ext.args = '--minAssignedFrags 1'
+    }
+
+}
diff --git a/modules/nf-core/salmon/quant/tests/tags.yml b/modules/nf-core/salmon/quant/tests/tags.yml
new file mode 100644
index 000000000..048d81642
--- /dev/null
+++ b/modules/nf-core/salmon/quant/tests/tags.yml
@@ -0,0 +1,2 @@
+salmon/quant:
+  - modules/nf-core/salmon/quant/**
diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index 6b53358ad..0bc37a601 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -5,9 +5,10 @@
 include { PICARD_COLLECTRNASEQMETRICS }                from '../../modules/local/picard/collectrnaseqmetrics/main'
 include { GATK4_MARKDUPLICATES }                       from '../../modules/nf-core/gatk4/markduplicates/main'
 include { PICARD_COLLECTINSERTSIZEMETRICS }            from '../../modules/nf-core/picard/collectinsertsizemetrics/main'
-
+ include { SALMON_QUANT }                              from '../../modules/nf-core/salmon/quant/main'
 workflow QC_WORKFLOW {
     take:
+        ch_reads_all
         ch_bam_sorted
         ch_bam_sorted_indexed
         ch_chrgtf
@@ -31,6 +32,8 @@ workflow QC_WORKFLOW {
         ch_versions = ch_versions.mix(PICARD_COLLECTINSERTSIZEMETRICS.out.versions)
         ch_insertsize_metrics = Channel.empty().mix(PICARD_COLLECTINSERTSIZEMETRICS.out.metrics)
 
+        SALMON_QUANT(ch_reads_all, salmon_index, ch_chrgtf.map { meta, gtf -> [ gtf ]}, transcript_fasta, true, '')
+
 
     emit:
         versions            = ch_versions
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 7847dd055..151849820 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -24,6 +24,7 @@ include { SAMTOOLS_FAIDX }                  from '../modules/nf-core/samtools/fa
 include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genomegenerate/main'
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
 include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
+include { SALMON_INDEX }                    from '../modules/nf-core/salmon/index/main'
 
 /*
 ========================================================================================
@@ -47,6 +48,7 @@ workflow BUILD_REFERENCES {
 
     GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
 
+    SALMON_INDEX(ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf)
 
     if (params.starindex || params.all || params.starfusion || params.arriba) {
         STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 7483f1be5..0c23a15c1 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -199,6 +199,7 @@ workflow RNAFUSION {
 
     //QC
     QC_WORKFLOW (
+        ch_reads_all,
         STARFUSION_WORKFLOW.out.ch_bam_sorted,
         STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
         ch_chrgtf,

From 89ade662e07eb87a3e27fcc008cfbc8693ff79c0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 14 May 2024 11:16:24 +0200
Subject: [PATCH 014/887] remove samplesheet check conf

---
 conf/modules.config | 8 --------
 1 file changed, 8 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 9d4ca62b1..965b31335 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -172,14 +172,6 @@ process {
         ]
     }
 
-    withName: 'SAMPLESHEET_CHECK' {
-        publishDir = [
-            path: { "${params.outdir}/pipeline_info" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
     withName: 'SAMTOOLS_FAIDX' {
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },

From 71f8caf804f757b73523a30ef44c558fef5d26a7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 10:37:47 +0200
Subject: [PATCH 015/887] tests

---
 conf/modules.config                           |  9 +++
 modules.json                                  | 10 +++
 modules/local/ensembl/main.nf                 | 10 ++-
 modules/local/gffread.nf                      | 50 +++++++++++++
 modules/nf-core/gunzip/environment.yml        |  7 ++
 modules/nf-core/gunzip/main.nf                | 48 +++++++++++++
 modules/nf-core/gunzip/meta.yml               | 39 ++++++++++
 modules/nf-core/gunzip/tests/main.nf.test     | 36 ++++++++++
 .../nf-core/gunzip/tests/main.nf.test.snap    | 31 ++++++++
 modules/nf-core/gunzip/tests/tags.yml         |  2 +
 .../rsem/preparereference/environment.yml     |  8 +++
 modules/nf-core/rsem/preparereference/main.nf | 72 +++++++++++++++++++
 .../nf-core/rsem/preparereference/meta.yml    | 42 +++++++++++
 .../rsem/preparereference/tests/main.nf.test  | 32 +++++++++
 .../preparereference/tests/main.nf.test.snap  | 35 +++++++++
 workflows/build_references.nf                 | 72 ++++++++++---------
 16 files changed, 468 insertions(+), 35 deletions(-)
 create mode 100644 modules/local/gffread.nf
 create mode 100644 modules/nf-core/gunzip/environment.yml
 create mode 100644 modules/nf-core/gunzip/main.nf
 create mode 100644 modules/nf-core/gunzip/meta.yml
 create mode 100644 modules/nf-core/gunzip/tests/main.nf.test
 create mode 100644 modules/nf-core/gunzip/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/gunzip/tests/tags.yml
 create mode 100644 modules/nf-core/rsem/preparereference/environment.yml
 create mode 100644 modules/nf-core/rsem/preparereference/main.nf
 create mode 100644 modules/nf-core/rsem/preparereference/meta.yml
 create mode 100644 modules/nf-core/rsem/preparereference/tests/main.nf.test
 create mode 100644 modules/nf-core/rsem/preparereference/tests/main.nf.test.snap

diff --git a/conf/modules.config b/conf/modules.config
index 9d4ca62b1..bef67c2ca 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -122,6 +122,15 @@ process {
         ]
     }
 
+    withName: 'GFFREAD' {
+        ext.args = { '-w' }
+        publishDir = [
+            path: { "${params.genomes_base}/gffread" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
+
     withName: 'GTF_TO_REFFLAT' {
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },
diff --git a/modules.json b/modules.json
index 384c60a53..cb364b688 100644
--- a/modules.json
+++ b/modules.json
@@ -50,6 +50,11 @@
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
+                    "gunzip": {
+                        "branch": "master",
+                        "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
+                        "installed_by": ["modules"]
+                    },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
@@ -65,6 +70,11 @@
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
                         "installed_by": ["modules"]
                     },
+                    "rsem/preparereference": {
+                        "branch": "master",
+                        "git_sha": "301b088c7e9e00c4c80686411383f07173b54d69",
+                        "installed_by": ["modules"]
+                    },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "ffc101e1b84ef3df2e4e4a966e84b3c513ae5693",
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 4a782c0df..90ef0e470 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -17,7 +17,10 @@ process ENSEMBL_DOWNLOAD {
     tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.gtf")           , emit: gtf
     tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.chr.gtf")       , emit: chrgtf
     tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.cdna.all.fa.gz"), emit: transcript
-    path "versions.yml"                                                                                                                       , emit: versions
+    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz"), emit: primary_assembly
+    tuple val(meta), path("gencode.v37.primary_assembly.annotation.gtf"), emit: primary_assembly_gtf
+
+    path "versions.yml"                                            , emit: versions
 
 
     script:
@@ -28,12 +31,13 @@ process ENSEMBL_DOWNLOAD {
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz
-
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.primary_assembly.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.dna.primary_assembly.fa.gz
+    wget https://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_37/gencode.v37.primary_assembly.annotation.gtf.gz
     gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
 
-
+    gunzip gencode.v37.primary_assembly.annotation.gtf.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/local/gffread.nf b/modules/local/gffread.nf
new file mode 100644
index 000000000..98863efa5
--- /dev/null
+++ b/modules/local/gffread.nf
@@ -0,0 +1,50 @@
+process GFFREAD {
+    tag "$gff"
+    label 'process_low'
+
+    conda "bioconda::gffread=0.12.1"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0' :
+        'biocontainers/gffread:0.12.1--h8b12597_0' }"
+
+    input:
+    tuple val(meta), path(gff)
+    tuple val(meta2), path(fasta), path(fai)
+
+    output:
+    path "*_gffread_output.gtf", emit: gtf,        optional: true
+    path "*_gffread_output.fa"  , emit: tr_fasta,   optional: true
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args   = task.ext.args   ?: ''
+    def genome = fasta ? "-g ${fasta}" : ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def outp   = args.contains("-w") ? "-w ${prefix}_gffread_output.fa" : "-o ${prefix}_gffread_output.gtf"
+
+    """
+    gffread \\
+        $gff \\
+        $genome \\
+        $outp
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gffread: \$(gffread --version 2>&1)
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}_gffread"
+    """
+    touch ${prefix}_gffread_output.gtf
+    touch ${prefix}_gffread_output.fa
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gffread: \$(gffread --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml
new file mode 100644
index 000000000..25910b340
--- /dev/null
+++ b/modules/nf-core/gunzip/environment.yml
@@ -0,0 +1,7 @@
+name: gunzip
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - conda-forge::sed=4.7
diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf
new file mode 100644
index 000000000..468a6f287
--- /dev/null
+++ b/modules/nf-core/gunzip/main.nf
@@ -0,0 +1,48 @@
+process GUNZIP {
+    tag "$archive"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
+        'nf-core/ubuntu:20.04' }"
+
+    input:
+    tuple val(meta), path(archive)
+
+    output:
+    tuple val(meta), path("$gunzip"), emit: gunzip
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    gunzip = archive.toString() - '.gz'
+    """
+    # Not calling gunzip itself because it creates files
+    # with the original group ownership rather than the
+    # default one for that user / the work directory
+    gzip \\
+        -cd \\
+        $args \\
+        $archive \\
+        > $gunzip
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    gunzip = archive.toString() - '.gz'
+    """
+    touch $gunzip
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml
new file mode 100644
index 000000000..231034f2f
--- /dev/null
+++ b/modules/nf-core/gunzip/meta.yml
@@ -0,0 +1,39 @@
+name: gunzip
+description: Compresses and decompresses files.
+keywords:
+  - gunzip
+  - compression
+  - decompression
+tools:
+  - gunzip:
+      description: |
+        gzip is a file format and a software application used for file compression and decompression.
+      documentation: https://www.gnu.org/software/gzip/manual/gzip.html
+      licence: ["GPL-3.0-or-later"]
+input:
+  - meta:
+      type: map
+      description: |
+        Optional groovy Map containing meta information
+        e.g. [ id:'test', single_end:false ]
+  - archive:
+      type: file
+      description: File to be compressed/uncompressed
+      pattern: "*.*"
+output:
+  - gunzip:
+      type: file
+      description: Compressed/uncompressed file
+      pattern: "*.*"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@jfy133"
+maintainers:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@jfy133"
diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test
new file mode 100644
index 000000000..6406008ef
--- /dev/null
+++ b/modules/nf-core/gunzip/tests/main.nf.test
@@ -0,0 +1,36 @@
+nextflow_process {
+
+    name "Test Process GUNZIP"
+    script "../main.nf"
+    process "GUNZIP"
+    tag "gunzip"
+    tag "modules_nfcore"
+    tag "modules"
+
+    test("Should run without failures") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                        [],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                    ]
+                )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap
new file mode 100644
index 000000000..720fd9ff4
--- /dev/null
+++ b/modules/nf-core/gunzip/tests/main.nf.test.snap
@@ -0,0 +1,31 @@
+{
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    [
+                        [
+                            
+                        ],
+                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ],
+                "gunzip": [
+                    [
+                        [
+                            
+                        ],
+                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ]
+            }
+        ],
+        "timestamp": "2023-10-17T15:35:37.690477896"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml
new file mode 100644
index 000000000..fd3f69154
--- /dev/null
+++ b/modules/nf-core/gunzip/tests/tags.yml
@@ -0,0 +1,2 @@
+gunzip:
+  - modules/nf-core/gunzip/**
diff --git a/modules/nf-core/rsem/preparereference/environment.yml b/modules/nf-core/rsem/preparereference/environment.yml
new file mode 100644
index 000000000..c80e69106
--- /dev/null
+++ b/modules/nf-core/rsem/preparereference/environment.yml
@@ -0,0 +1,8 @@
+name: rsem_preparereference
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::rsem=1.3.3
+  - bioconda::star=2.7.10a
diff --git a/modules/nf-core/rsem/preparereference/main.nf b/modules/nf-core/rsem/preparereference/main.nf
new file mode 100644
index 000000000..44f76ebd6
--- /dev/null
+++ b/modules/nf-core/rsem/preparereference/main.nf
@@ -0,0 +1,72 @@
+process RSEM_PREPAREREFERENCE {
+    tag "$fasta"
+    label 'process_high'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' :
+        'biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' }"
+
+    input:
+    path fasta, stageAs: "rsem/*"
+    path gtf
+
+    output:
+    path "rsem"           , emit: index
+    path "*transcripts.fa", emit: transcript_fasta
+    path "versions.yml"   , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    def args_list = args.tokenize()
+    if (args_list.contains('--star')) {
+        args_list.removeIf { it.contains('--star') }
+        def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
+        """
+        STAR \\
+            --runMode genomeGenerate \\
+            --genomeDir rsem/ \\
+            --genomeFastaFiles $fasta \\
+            --sjdbGTFfile $gtf \\
+            --runThreadN $task.cpus \\
+            $memory \\
+            $args2
+
+        rsem-prepare-reference \\
+            --gtf $gtf \\
+            --num-threads $task.cpus \\
+            ${args_list.join(' ')} \\
+            $fasta \\
+            rsem/genome
+
+        cp rsem/genome.transcripts.fa .
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            rsem: \$(rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g")
+            star: \$(STAR --version | sed -e "s/STAR_//g")
+        END_VERSIONS
+        """
+    } else {
+        """
+        rsem-prepare-reference \\
+            --gtf $gtf \\
+            --num-threads $task.cpus \\
+            $args \\
+            $fasta \\
+            rsem/genome
+
+        cp rsem/genome.transcripts.fa .
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            rsem: \$(rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g")
+            star: \$(STAR --version | sed -e "s/STAR_//g")
+        END_VERSIONS
+        """
+    }
+}
diff --git a/modules/nf-core/rsem/preparereference/meta.yml b/modules/nf-core/rsem/preparereference/meta.yml
new file mode 100644
index 000000000..05aa44fe6
--- /dev/null
+++ b/modules/nf-core/rsem/preparereference/meta.yml
@@ -0,0 +1,42 @@
+name: rsem_preparereference
+description: Prepare a reference genome for RSEM
+keywords:
+  - rsem
+  - genome
+  - index
+tools:
+  - rseqc:
+      description: |
+        RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
+      homepage: https://github.com/deweylab/RSEM
+      documentation: https://github.com/deweylab/RSEM
+      doi: 10.1186/1471-2105-12-323
+      licence: ["GPL-3.0-or-later"]
+input:
+  - fasta:
+      type: file
+      description: The Fasta file of the reference genome
+      pattern: "*.{fasta,fa}"
+  - gtf:
+      type: file
+      description: The GTF file of the reference genome
+      pattern: "*.gtf"
+output:
+  - rsem:
+      type: directory
+      description: RSEM index directory
+      pattern: "rsem"
+  - transcript_fasta:
+      type: file
+      description: Fasta file of transcripts
+      pattern: "rsem/*transcripts.fa"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@kevinmenden"
+maintainers:
+  - "@drpatelh"
+  - "@kevinmenden"
diff --git a/modules/nf-core/rsem/preparereference/tests/main.nf.test b/modules/nf-core/rsem/preparereference/tests/main.nf.test
new file mode 100644
index 000000000..4353d4d9b
--- /dev/null
+++ b/modules/nf-core/rsem/preparereference/tests/main.nf.test
@@ -0,0 +1,32 @@
+nextflow_process {
+
+    name "Test Process RSEM_PREPAREREFERENCE"
+    script "../main.nf"
+    process "RSEM_PREPAREREFERENCE"
+
+    test("homo_sapiens") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true))
+                input[1] = Channel.of(file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true))
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.index).match("index")},
+                { assert snapshot(process.out.transcript_fasta).match("transcript_fasta")},
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/rsem/preparereference/tests/main.nf.test.snap b/modules/nf-core/rsem/preparereference/tests/main.nf.test.snap
new file mode 100644
index 000000000..02510659a
--- /dev/null
+++ b/modules/nf-core/rsem/preparereference/tests/main.nf.test.snap
@@ -0,0 +1,35 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,517611c42f6354d3609db1b35fffa397"
+            ]
+        ],
+        "timestamp": "2023-11-22T13:16:49.170587"
+    },
+    "index": {
+        "content": [
+            [
+                [
+                    "genome.chrlist:md5,b190587cae0531f3cf25552d8aa674db",
+                    "genome.fasta:md5,f315020d899597c1b57e5fe9f60f4c3e",
+                    "genome.grp:md5,c2848a8b6d495956c11ec53efc1de67e",
+                    "genome.idx.fa:md5,050c521a2719c2ae48267c1e65218f29",
+                    "genome.n2g.idx.fa:md5,050c521a2719c2ae48267c1e65218f29",
+                    "genome.seq:md5,94da0c6b88c33e63c9a052a11f4f57c1",
+                    "genome.ti:md5,c9e4ae8d4d13a504eec2acf1b8589a66",
+                    "genome.transcripts.fa:md5,050c521a2719c2ae48267c1e65218f29"
+                ]
+            ]
+        ],
+        "timestamp": "2023-11-22T13:16:49.140398"
+    },
+    "transcript_fasta": {
+        "content": [
+            [
+                "genome.transcripts.fa:md5,050c521a2719c2ae48267c1e65218f29"
+            ]
+        ],
+        "timestamp": "2023-11-22T13:16:49.159946"
+    }
+}
\ No newline at end of file
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 151849820..293f00482 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -25,7 +25,9 @@ include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genome
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
 include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
 include { SALMON_INDEX }                    from '../modules/nf-core/salmon/index/main'
-
+include { RSEM_PREPAREREFERENCE }           from '../modules/nf-core/rsem/preparereference'
+include { GFFREAD                                      } from '../modules/local/gffread'
+ include { GUNZIP } from '../modules/nf-core/gunzip/main'
 /*
 ========================================================================================
     RUN MAIN WORKFLOW
@@ -37,48 +39,54 @@ workflow BUILD_REFERENCES {
     def fake_meta = [:]
     fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
     ENSEMBL_DOWNLOAD( params.ensembl_version, params.genome, fake_meta )
-    HGNC_DOWNLOAD( )
+    // HGNC_DOWNLOAD( )
+    GUNZIP(ENSEMBL_DOWNLOAD.out.primary_assembly)
+
+
+    SAMTOOLS_FAIDX(GUNZIP.out.gunzip, [[],[]])
+    // GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.fasta)
 
+    // RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
+    // CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
 
-    SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.fasta, [[],[]])
-    GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.fasta)
+    // GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
 
-    RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
-    CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
+    ch_fasta_fai =(GUNZIP.out.gunzip).join(SAMTOOLS_FAIDX.out.fai)
+    GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ch_fasta_fai)
+    SALMON_INDEX(GUNZIP.out.gunzip.map { meta, fasta -> [ fasta ] }, GFFREAD.out.tr_fasta)
 
-    GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
+    // RSEM_PREPAREREFERENCE(ENSEMBL_DOWNLOAD.out.fasta.map { meta, fasta -> [ fasta ] }, ENSEMBL_DOWNLOAD.out.gtf.map { meta, gtf -> [ gtf ] })
 
-    SALMON_INDEX(ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf)
 
-    if (params.starindex || params.all || params.starfusion || params.arriba) {
-        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
-    }
+    // if (params.starindex || params.all || params.starfusion || params.arriba) {
+    //     STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+    // }
 
-    if (params.arriba || params.all) {
-        ARRIBA_DOWNLOAD()
-    }
+    // if (params.arriba || params.all) {
+    //     ARRIBA_DOWNLOAD()
+    // }
 
-    if (params.fusioncatcher || params.all) {
-        FUSIONCATCHER_DOWNLOAD()
-    }
+    // if (params.fusioncatcher || params.all) {
+    //     FUSIONCATCHER_DOWNLOAD()
+    // }
 
-    if (params.starfusion || params.all) {
-        if (params.starfusion_build){
-            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
-        } else {
-            STARFUSION_DOWNLOAD()
-        }
-    }
+    // if (params.starfusion || params.all) {
+    //     if (params.starfusion_build){
+    //         STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
+    //     } else {
+    //         STARFUSION_DOWNLOAD()
+    //     }
+    // }
 
-    if (params.starfusion_build){
-        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
-    } else {
-        GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
-    }
+    // if (params.starfusion_build){
+    //     GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
+    // } else {
+    //     GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
+    // }
 
-    if (params.fusionreport || params.all) {
-        FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
-    }
+    // if (params.fusionreport || params.all) {
+    //     FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
+    // }
 
 }
 

From 0a87206220786b38cf712d32af3e82f07145cbd5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 11:08:05 +0200
Subject: [PATCH 016/887] update

---
 modules.json                                  | 118 +++++---
 modules/nf-core/gffread/environment.yml       |   7 +
 modules/nf-core/gffread/main.nf               |  60 ++++
 modules/nf-core/gffread/meta.yml              |  55 ++++
 modules/nf-core/gffread/tests/main.nf.test    | 223 ++++++++++++++
 .../nf-core/gffread/tests/main.nf.test.snap   | 272 ++++++++++++++++++
 .../gffread/tests/nextflow-fasta.config       |   5 +
 .../gffread/tests/nextflow-gff3.config        |   5 +
 modules/nf-core/gffread/tests/nextflow.config |   5 +
 modules/nf-core/gffread/tests/tags.yml        |   2 +
 .../rsem/preparereference/environment.yml     |   8 -
 modules/nf-core/rsem/preparereference/main.nf |  72 -----
 .../nf-core/rsem/preparereference/meta.yml    |  42 ---
 .../rsem/preparereference/tests/main.nf.test  |  32 ---
 .../preparereference/tests/main.nf.test.snap  |  35 ---
 workflows/build_references.nf                 |   3 +-
 16 files changed, 721 insertions(+), 223 deletions(-)
 create mode 100644 modules/nf-core/gffread/environment.yml
 create mode 100644 modules/nf-core/gffread/main.nf
 create mode 100644 modules/nf-core/gffread/meta.yml
 create mode 100644 modules/nf-core/gffread/tests/main.nf.test
 create mode 100644 modules/nf-core/gffread/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/gffread/tests/nextflow-fasta.config
 create mode 100644 modules/nf-core/gffread/tests/nextflow-gff3.config
 create mode 100644 modules/nf-core/gffread/tests/nextflow.config
 create mode 100644 modules/nf-core/gffread/tests/tags.yml
 delete mode 100644 modules/nf-core/rsem/preparereference/environment.yml
 delete mode 100644 modules/nf-core/rsem/preparereference/main.nf
 delete mode 100644 modules/nf-core/rsem/preparereference/meta.yml
 delete mode 100644 modules/nf-core/rsem/preparereference/tests/main.nf.test
 delete mode 100644 modules/nf-core/rsem/preparereference/tests/main.nf.test.snap

diff --git a/modules.json b/modules.json
index cb364b688..4aa2ebe57 100644
--- a/modules.json
+++ b/modules.json
@@ -8,122 +8,170 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba": {
                         "branch": "master",
                         "git_sha": "ea9e2892a9d12e8769402f12096219942bcf6536",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
+                    },
+                    "gffread": {
+                        "branch": "master",
+                        "git_sha": "6c996d7fbe0816dcbb68ce587ad5f873313682a1",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gunzip": {
                         "branch": "master",
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
-                    },
-                    "rsem/preparereference": {
-                        "branch": "master",
-                        "git_sha": "301b088c7e9e00c4c80686411383f07173b54d69",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "ffc101e1b84ef3df2e4e4a966e84b3c513ae5693",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "03a8562231d575c313266c193a980594b941e3ea",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "9f6b233518f7d9ecdcf24b798b7e491db5424273",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "0e98289b5bec6e3f8f588a8a9d05e8aacc1179a0",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "b0dcb44b018d9b2bcb35b1abb7bcd34061bc5a6d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "b0dcb44b018d9b2bcb35b1abb7bcd34061bc5a6d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -132,20 +180,26 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfvalidation_plugin": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/gffread/environment.yml b/modules/nf-core/gffread/environment.yml
new file mode 100644
index 000000000..c6df58ad0
--- /dev/null
+++ b/modules/nf-core/gffread/environment.yml
@@ -0,0 +1,7 @@
+name: gffread
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - bioconda::gffread=0.12.7
diff --git a/modules/nf-core/gffread/main.nf b/modules/nf-core/gffread/main.nf
new file mode 100644
index 000000000..da55cbab7
--- /dev/null
+++ b/modules/nf-core/gffread/main.nf
@@ -0,0 +1,60 @@
+process GFFREAD {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/gffread:0.12.7--hdcf5f25_4' :
+        'biocontainers/gffread:0.12.7--hdcf5f25_4' }"
+
+    input:
+    tuple val(meta), path(gff)
+    path fasta
+
+    output:
+    tuple val(meta), path("*.gtf")  , emit: gtf             , optional: true
+    tuple val(meta), path("*.gff3") , emit: gffread_gff     , optional: true
+    tuple val(meta), path("*.fasta"), emit: gffread_fasta   , optional: true
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args        = task.ext.args             ?: ''
+    def prefix      = task.ext.prefix           ?: "${meta.id}"
+    def extension   = args.contains("-T")       ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' )
+    def fasta_arg   = fasta                     ? "-g $fasta" : ''
+    def output_name = "${prefix}.${extension}"
+    def output      = extension == "fasta"      ? "$output_name" : "-o $output_name"
+    def args_sorted = args.replaceAll(/(.*)(-[wxy])(.*)/) { all, pre, param, post -> "$pre $post $param" }.trim()
+    // args_sorted  = Move '-w', '-x', and '-y' to the end of the args string as gffread expects the file name after these parameters
+    if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    gffread \\
+        $gff \\
+        $fasta_arg \\
+        $args_sorted \\
+        $output
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gffread: \$(gffread --version 2>&1)
+    END_VERSIONS
+    """
+
+    stub:
+    def args        = task.ext.args             ?: ''
+    def prefix      = task.ext.prefix           ?: "${meta.id}"
+    def extension   = args.contains("-T")       ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' )
+    def output_name = "${prefix}.${extension}"
+    if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    """
+    touch $output_name
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gffread: \$(gffread --version 2>&1)
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/gffread/meta.yml b/modules/nf-core/gffread/meta.yml
new file mode 100644
index 000000000..c06028208
--- /dev/null
+++ b/modules/nf-core/gffread/meta.yml
@@ -0,0 +1,55 @@
+name: gffread
+description: Validate, filter, convert and perform various other operations on GFF files
+keywords:
+  - gff
+  - conversion
+  - validation
+tools:
+  - gffread:
+      description: GFF/GTF utility providing format conversions, region filtering, FASTA sequence extraction and more.
+      homepage: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread
+      documentation: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread
+      tool_dev_url: https://github.com/gpertea/gffread
+      doi: 10.12688/f1000research.23297.1
+      licence: ["MIT"]
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing meta data
+        e.g. [ id:'test' ]
+  - gff:
+      type: file
+      description: A reference file in either the GFF3, GFF2 or GTF format.
+      pattern: "*.{gff, gtf}"
+  - fasta:
+      type: file
+      description: A multi-fasta file with the genomic sequences
+      pattern: "*.{fasta,fa,faa,fas,fsa}"
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing meta data
+        e.g. [ id:'test' ]
+  - gtf:
+      type: file
+      description: GTF file resulting from the conversion of the GFF input file if '-T' argument is present
+      pattern: "*.{gtf}"
+  - gffread_gff:
+      type: file
+      description: GFF3 file resulting from the conversion of the GFF input file if '-T' argument is absent
+      pattern: "*.gff3"
+  - gffread_fasta:
+      type: file
+      description: Fasta file produced when either of '-w', '-x', '-y' parameters is present
+      pattern: "*.fasta"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@edmundmiller"
+maintainers:
+  - "@edmundmiller"
+  - "@gallvp"
diff --git a/modules/nf-core/gffread/tests/main.nf.test b/modules/nf-core/gffread/tests/main.nf.test
new file mode 100644
index 000000000..4cd13dcd3
--- /dev/null
+++ b/modules/nf-core/gffread/tests/main.nf.test
@@ -0,0 +1,223 @@
+nextflow_process {
+
+    name "Test Process GFFREAD"
+    script "../main.nf"
+    process "GFFREAD"
+
+    tag "gffread"
+    tag "modules_nfcore"
+    tag "modules"
+
+    test("sarscov2-gff3-gtf") {
+
+        config "./nextflow.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gffread_gff == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-gtf-stub") {
+
+        options '-stub'
+        config "./nextflow.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gffread_gff == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-gff3") {
+
+        config "./nextflow-gff3.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-gff3-stub") {
+
+        options '-stub'
+        config "./nextflow-gff3.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_fasta == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-fasta") {
+
+        config "./nextflow-fasta.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_gff == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-fasta-stub") {
+
+        options '-stub'
+        config "./nextflow-fasta.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'test'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() },
+                { assert process.out.gtf == [] },
+                { assert process.out.gffread_gff == [] }
+            )
+        }
+
+    }
+
+    test("sarscov2-gff3-fasta-fail-catch") {
+
+        options '-stub'
+        config "./nextflow-fasta.config"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = [
+                    [id: 'genome'],
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert ! process.success },
+                { assert process.stdout.toString().contains("Input and output names are the same") }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/gffread/tests/main.nf.test.snap b/modules/nf-core/gffread/tests/main.nf.test.snap
new file mode 100644
index 000000000..15262320d
--- /dev/null
+++ b/modules/nf-core/gffread/tests/main.nf.test.snap
@@ -0,0 +1,272 @@
+{
+    "sarscov2-gff3-gtf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T10:48:56.496187"
+    },
+    "sarscov2-gff3-gff3": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91"
+                    ]
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T10:49:00.892782"
+    },
+    "sarscov2-gff3-gtf-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T11:11:26.975666"
+    },
+    "sarscov2-gff3-fasta-stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T11:11:44.34792"
+    },
+    "sarscov2-gff3-gff3-stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    
+                ],
+                "gffread_gff": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T11:11:35.221671"
+    },
+    "sarscov2-gff3-fasta": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ],
+                "gffread_fasta": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a"
+                    ]
+                ],
+                "gffread_gff": [
+                    
+                ],
+                "gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-09T10:54:02.88143"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/gffread/tests/nextflow-fasta.config b/modules/nf-core/gffread/tests/nextflow-fasta.config
new file mode 100644
index 000000000..ac6cb1484
--- /dev/null
+++ b/modules/nf-core/gffread/tests/nextflow-fasta.config
@@ -0,0 +1,5 @@
+process {
+    withName: GFFREAD {
+        ext.args = '-w -S'
+    }
+}
diff --git a/modules/nf-core/gffread/tests/nextflow-gff3.config b/modules/nf-core/gffread/tests/nextflow-gff3.config
new file mode 100644
index 000000000..afe0830e5
--- /dev/null
+++ b/modules/nf-core/gffread/tests/nextflow-gff3.config
@@ -0,0 +1,5 @@
+process {
+    withName: GFFREAD {
+        ext.args = ''
+    }
+}
diff --git a/modules/nf-core/gffread/tests/nextflow.config b/modules/nf-core/gffread/tests/nextflow.config
new file mode 100644
index 000000000..74b25094f
--- /dev/null
+++ b/modules/nf-core/gffread/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: GFFREAD {
+        ext.args = '-T'
+    }
+}
diff --git a/modules/nf-core/gffread/tests/tags.yml b/modules/nf-core/gffread/tests/tags.yml
new file mode 100644
index 000000000..055760656
--- /dev/null
+++ b/modules/nf-core/gffread/tests/tags.yml
@@ -0,0 +1,2 @@
+gffread:
+  - modules/nf-core/gffread/**
diff --git a/modules/nf-core/rsem/preparereference/environment.yml b/modules/nf-core/rsem/preparereference/environment.yml
deleted file mode 100644
index c80e69106..000000000
--- a/modules/nf-core/rsem/preparereference/environment.yml
+++ /dev/null
@@ -1,8 +0,0 @@
-name: rsem_preparereference
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - bioconda::rsem=1.3.3
-  - bioconda::star=2.7.10a
diff --git a/modules/nf-core/rsem/preparereference/main.nf b/modules/nf-core/rsem/preparereference/main.nf
deleted file mode 100644
index 44f76ebd6..000000000
--- a/modules/nf-core/rsem/preparereference/main.nf
+++ /dev/null
@@ -1,72 +0,0 @@
-process RSEM_PREPAREREFERENCE {
-    tag "$fasta"
-    label 'process_high'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' :
-        'biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' }"
-
-    input:
-    path fasta, stageAs: "rsem/*"
-    path gtf
-
-    output:
-    path "rsem"           , emit: index
-    path "*transcripts.fa", emit: transcript_fasta
-    path "versions.yml"   , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    def args_list = args.tokenize()
-    if (args_list.contains('--star')) {
-        args_list.removeIf { it.contains('--star') }
-        def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
-        """
-        STAR \\
-            --runMode genomeGenerate \\
-            --genomeDir rsem/ \\
-            --genomeFastaFiles $fasta \\
-            --sjdbGTFfile $gtf \\
-            --runThreadN $task.cpus \\
-            $memory \\
-            $args2
-
-        rsem-prepare-reference \\
-            --gtf $gtf \\
-            --num-threads $task.cpus \\
-            ${args_list.join(' ')} \\
-            $fasta \\
-            rsem/genome
-
-        cp rsem/genome.transcripts.fa .
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            rsem: \$(rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g")
-            star: \$(STAR --version | sed -e "s/STAR_//g")
-        END_VERSIONS
-        """
-    } else {
-        """
-        rsem-prepare-reference \\
-            --gtf $gtf \\
-            --num-threads $task.cpus \\
-            $args \\
-            $fasta \\
-            rsem/genome
-
-        cp rsem/genome.transcripts.fa .
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            rsem: \$(rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g")
-            star: \$(STAR --version | sed -e "s/STAR_//g")
-        END_VERSIONS
-        """
-    }
-}
diff --git a/modules/nf-core/rsem/preparereference/meta.yml b/modules/nf-core/rsem/preparereference/meta.yml
deleted file mode 100644
index 05aa44fe6..000000000
--- a/modules/nf-core/rsem/preparereference/meta.yml
+++ /dev/null
@@ -1,42 +0,0 @@
-name: rsem_preparereference
-description: Prepare a reference genome for RSEM
-keywords:
-  - rsem
-  - genome
-  - index
-tools:
-  - rseqc:
-      description: |
-        RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
-      homepage: https://github.com/deweylab/RSEM
-      documentation: https://github.com/deweylab/RSEM
-      doi: 10.1186/1471-2105-12-323
-      licence: ["GPL-3.0-or-later"]
-input:
-  - fasta:
-      type: file
-      description: The Fasta file of the reference genome
-      pattern: "*.{fasta,fa}"
-  - gtf:
-      type: file
-      description: The GTF file of the reference genome
-      pattern: "*.gtf"
-output:
-  - rsem:
-      type: directory
-      description: RSEM index directory
-      pattern: "rsem"
-  - transcript_fasta:
-      type: file
-      description: Fasta file of transcripts
-      pattern: "rsem/*transcripts.fa"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@kevinmenden"
-maintainers:
-  - "@drpatelh"
-  - "@kevinmenden"
diff --git a/modules/nf-core/rsem/preparereference/tests/main.nf.test b/modules/nf-core/rsem/preparereference/tests/main.nf.test
deleted file mode 100644
index 4353d4d9b..000000000
--- a/modules/nf-core/rsem/preparereference/tests/main.nf.test
+++ /dev/null
@@ -1,32 +0,0 @@
-nextflow_process {
-
-    name "Test Process RSEM_PREPAREREFERENCE"
-    script "../main.nf"
-    process "RSEM_PREPAREREFERENCE"
-
-    test("homo_sapiens") {
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-            process {
-                """
-                input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true))
-                input[1] = Channel.of(file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true))
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out.index).match("index")},
-                { assert snapshot(process.out.transcript_fasta).match("transcript_fasta")},
-                { assert snapshot(process.out.versions).match("versions") }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/rsem/preparereference/tests/main.nf.test.snap b/modules/nf-core/rsem/preparereference/tests/main.nf.test.snap
deleted file mode 100644
index 02510659a..000000000
--- a/modules/nf-core/rsem/preparereference/tests/main.nf.test.snap
+++ /dev/null
@@ -1,35 +0,0 @@
-{
-    "versions": {
-        "content": [
-            [
-                "versions.yml:md5,517611c42f6354d3609db1b35fffa397"
-            ]
-        ],
-        "timestamp": "2023-11-22T13:16:49.170587"
-    },
-    "index": {
-        "content": [
-            [
-                [
-                    "genome.chrlist:md5,b190587cae0531f3cf25552d8aa674db",
-                    "genome.fasta:md5,f315020d899597c1b57e5fe9f60f4c3e",
-                    "genome.grp:md5,c2848a8b6d495956c11ec53efc1de67e",
-                    "genome.idx.fa:md5,050c521a2719c2ae48267c1e65218f29",
-                    "genome.n2g.idx.fa:md5,050c521a2719c2ae48267c1e65218f29",
-                    "genome.seq:md5,94da0c6b88c33e63c9a052a11f4f57c1",
-                    "genome.ti:md5,c9e4ae8d4d13a504eec2acf1b8589a66",
-                    "genome.transcripts.fa:md5,050c521a2719c2ae48267c1e65218f29"
-                ]
-            ]
-        ],
-        "timestamp": "2023-11-22T13:16:49.140398"
-    },
-    "transcript_fasta": {
-        "content": [
-            [
-                "genome.transcripts.fa:md5,050c521a2719c2ae48267c1e65218f29"
-            ]
-        ],
-        "timestamp": "2023-11-22T13:16:49.159946"
-    }
-}
\ No newline at end of file
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 293f00482..db28c2a8a 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -25,9 +25,8 @@ include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genome
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
 include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
 include { SALMON_INDEX }                    from '../modules/nf-core/salmon/index/main'
-include { RSEM_PREPAREREFERENCE }           from '../modules/nf-core/rsem/preparereference'
 include { GFFREAD                                      } from '../modules/local/gffread'
- include { GUNZIP } from '../modules/nf-core/gunzip/main'
+include { GUNZIP } from '../modules/nf-core/gunzip/main'
 /*
 ========================================================================================
     RUN MAIN WORKFLOW

From 3be24cb69c63573a922da61a49a7bc85dbbbe301 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 11:09:28 +0200
Subject: [PATCH 017/887] fix merge conflicts

---
 modules.json | 9 +--------
 1 file changed, 1 insertion(+), 8 deletions(-)

diff --git a/modules.json b/modules.json
index 52ca63ff1..b9ae28bac 100644
--- a/modules.json
+++ b/modules.json
@@ -42,15 +42,8 @@
                     },
                     "fastqc": {
                         "branch": "master",
-<<<<<<< HEAD
-                        "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c",
-                        "installed_by": [
-                            "modules"
-                        ]
-=======
                         "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
                         "installed_by": ["modules"]
->>>>>>> 973ee3ba5bd0fa00178dcfb917bb8cfab36d0243
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
@@ -212,4 +205,4 @@
             }
         }
     }
-}
\ No newline at end of file
+}

From 002b9288486488f19f15523b138757cda7eec1f5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 13:25:21 +0200
Subject: [PATCH 018/887] cleanup

---
 conf/modules.config                           |  16 +++
 modules.json                                  | 119 ++++-----------
 modules/local/ensembl/main.nf                 |  32 ++---
 modules/nf-core/salmon/index/main.nf          |  25 ++++
 .../nf-core/salmon/index/tests/main.nf.test   |  24 ++++
 .../salmon/index/tests/main.nf.test.snap      |  12 ++
 modules/nf-core/salmon/quant/main.nf          |  12 ++
 .../nf-core/salmon/quant/tests/main.nf.test   | 136 ++++++++++++++++++
 .../salmon/quant/tests/main.nf.test.snap      |  75 ++++++++++
 nextflow.config                               |   1 +
 nextflow_schema.json                          |   5 +
 workflows/build_references.nf                 |  93 ++++++------
 workflows/rnafusion.nf                        |   7 +
 13 files changed, 399 insertions(+), 158 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 75b957ec8..696889c8e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -181,6 +181,22 @@ process {
         ]
     }
 
+    withName: 'SALMON_INDEX' {
+        publishDir = [
+            path: { "${params.genomes_base}/salmon" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
+
+    withName: 'SALMON_QUANT' {
+        publishDir = [
+            path: { "${params.outdir}/salmon" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
+
     withName: 'SAMTOOLS_FAIDX' {
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },
diff --git a/modules.json b/modules.json
index b9ae28bac..4157b3177 100644
--- a/modules.json
+++ b/modules.json
@@ -8,37 +8,27 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba": {
                         "branch": "master",
-                        "git_sha": "ea9e2892a9d12e8769402f12096219942bcf6536",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "bbaeb3a3149def237b9d0acb9ab749ae98088e29",
+                        "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882",
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
@@ -48,128 +38,92 @@
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "6c996d7fbe0816dcbb68ce587ad5f873313682a1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gunzip": {
                         "branch": "master",
                         "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
-                        "git_sha": "ffc101e1b84ef3df2e4e4a966e84b3c513ae5693",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
+                        "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
-                        "git_sha": "03a8562231d575c313266c193a980594b941e3ea",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "9f6b233518f7d9ecdcf24b798b7e491db5424273",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "0e98289b5bec6e3f8f588a8a9d05e8aacc1179a0",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
-                        "git_sha": "b0dcb44b018d9b2bcb35b1abb7bcd34061bc5a6d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "5756b12b1fab0c07f05b9ad2d5d5c262b1a2c1ef",
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "b0dcb44b018d9b2bcb35b1abb7bcd34061bc5a6d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "b1b959609bda44341120aed1766329909f54b8d0",
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -178,28 +132,17 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-<<<<<<< HEAD
-                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
-=======
                         "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3",
                         "installed_by": ["subworkflows"]
->>>>>>> 973ee3ba5bd0fa00178dcfb917bb8cfab36d0243
                     },
                     "utils_nfvalidation_plugin": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 90ef0e470..8297e2901 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -13,48 +13,40 @@ process ENSEMBL_DOWNLOAD {
     val meta
 
     output:
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.all.fa")        , emit: fasta
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.gtf")           , emit: gtf
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.chr.gtf")       , emit: chrgtf
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.cdna.all.fa.gz"), emit: transcript
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz"), emit: primary_assembly
-    tuple val(meta), path("gencode.v37.primary_assembly.annotation.gtf"), emit: primary_assembly_gtf
-
-    path "versions.yml"                                            , emit: versions
+    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.gtf")                       , emit: gtf
+    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa")   , emit: primary_assembly
+    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.chr.gtf")                   , emit: chrgtf
+    path "versions.yml"                                                                          , emit: versions
 
 
     script:
     """
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz
-
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz
     wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.primary_assembly.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.dna.primary_assembly.fa.gz
-    wget https://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_37/gencode.v37.primary_assembly.annotation.gtf.gz
-    gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa
+    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
+
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
+    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.dna.primary_assembly.fa.gz
     gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
 
-    gunzip gencode.v37.primary_assembly.annotation.gtf.gz
-
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
+
     END_VERSIONS
     """
 
     stub:
     """
-    touch "Homo_sapiens.${genome}.${ensembl_version}.all.fa"
     touch "Homo_sapiens.${genome}.${ensembl_version}.gtf"
-    touch "Homo_sapiens.${genome}.${ensembl_version}.chr.gtf"
-    touch "Homo_sapiens.${genome}.${ensembl_version}.cdna.all.fa.gz"
+    touch "Homo_sapiens.${params.genome}.${ensembl_version}.dna.primary_assembly.fa"
+    touch "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
     END_VERSIONS
     """
 
diff --git a/modules/nf-core/salmon/index/main.nf b/modules/nf-core/salmon/index/main.nf
index 88d9cf14a..e755d9a34 100644
--- a/modules/nf-core/salmon/index/main.nf
+++ b/modules/nf-core/salmon/index/main.nf
@@ -44,4 +44,29 @@ process SALMON_INDEX {
         salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
     END_VERSIONS
     """
+
+    stub:
+    """
+    mkdir salmon
+    touch salmon/complete_ref_lens.bin
+    touch salmon/ctable.bin
+    touch salmon/ctg_offsets.bin
+    touch salmon/duplicate_clusters.tsv
+    touch salmon/info.json
+    touch salmon/mphf.bin
+    touch salmon/pos.bin
+    touch salmon/pre_indexing.log
+    touch salmon/rank.bin
+    touch salmon/refAccumLengths.bin
+    touch salmon/ref_indexing.log
+    touch salmon/reflengths.bin
+    touch salmon/refseq.bin
+    touch salmon/seq.bin
+    touch salmon/versionInfo.json
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/salmon/index/tests/main.nf.test b/modules/nf-core/salmon/index/tests/main.nf.test
index 538b231b4..16b3c1a79 100644
--- a/modules/nf-core/salmon/index/tests/main.nf.test
+++ b/modules/nf-core/salmon/index/tests/main.nf.test
@@ -32,4 +32,28 @@ nextflow_process {
 
     }
 
+     test("sarscov2 stub") {
+        options "-stub"
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.fasta", checkIfExists: true)])
+                input[1] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.index.get(0)).exists() },
+                { assert snapshot(process.out.versions).match("versions stub") }
+            )
+        }
+
+    }
+
 }
\ No newline at end of file
diff --git a/modules/nf-core/salmon/index/tests/main.nf.test.snap b/modules/nf-core/salmon/index/tests/main.nf.test.snap
index 94edf3903..703e455c5 100644
--- a/modules/nf-core/salmon/index/tests/main.nf.test.snap
+++ b/modules/nf-core/salmon/index/tests/main.nf.test.snap
@@ -10,5 +10,17 @@
             "nextflow": "23.10.1"
         },
         "timestamp": "2023-11-22T14:26:33.32036"
+    },
+    "versions stub": {
+        "content": [
+            [
+                "versions.yml:md5,563eeafb4577be0b13801d7021c0bf42"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-23T09:47:58.828124"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index e115d39cf..e177b42ac 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -76,4 +76,16 @@ process SALMON_QUANT {
         salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
     END_VERSIONS
     """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    mkdir ${prefix}
+    touch ${prefix}_meta_info.json
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g")
+    END_VERSIONS
+    """
 }
diff --git a/modules/nf-core/salmon/quant/tests/main.nf.test b/modules/nf-core/salmon/quant/tests/main.nf.test
index 04e61e2b3..b387fac2a 100644
--- a/modules/nf-core/salmon/quant/tests/main.nf.test
+++ b/modules/nf-core/salmon/quant/tests/main.nf.test
@@ -54,6 +54,38 @@ nextflow_process {
 
     }
 
+    test("sarscov2 - single_end stub") {
+        options "-stub"
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: true ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = ''
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_single_end_stub") }
+            )
+        }
+
+    }
+
     test("sarscov2 - single_end lib type A") {
 
         when {
@@ -86,6 +118,38 @@ nextflow_process {
 
     }
 
+    test("sarscov2 - single_end lib type A stub") {
+        options "-stub"
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: true ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = 'A'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_single_end_lib_type_a_stub") }
+            )
+        }
+
+    }
+
     test("sarscov2 - pair_end") {
 
         when {
@@ -121,6 +185,41 @@ nextflow_process {
 
     }
 
+    test("sarscov2 - pair_end stub") {
+        options "-stub"
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: true ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
+                    ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = ''
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_pair_end stub") }
+            )
+        }
+
+    }
+
     test("sarscov2 - pair_end multiple") {
 
         when {
@@ -157,4 +256,41 @@ nextflow_process {
         }
 
     }
+
+    test("sarscov2 - pair_end multiple stub") {
+        options "-stub"
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test2_1.fastq.gz", checkIfExists: true),
+                        file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test2_2.fastq.gz", checkIfExists: true)
+                    ]
+                ])
+                input[1] = SALMON_INDEX.out.index
+                input[2] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)])
+                input[3] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                input[4] = false
+                input[5] = ''
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.json_info.get(0).get(1)).exists() },
+                { assert path(process.out.results.get(0).get(1)).exists() },
+                { assert snapshot(process.out.versions).match("versions_pair_end_multiple_stub") }
+            )
+        }
+
+    }
 }
diff --git a/modules/nf-core/salmon/quant/tests/main.nf.test.snap b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
index a1ec792e0..a80bc8aa8 100644
--- a/modules/nf-core/salmon/quant/tests/main.nf.test.snap
+++ b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
@@ -1,4 +1,43 @@
 {
+    "versions_single_end_lib_type_a_stub": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-24T14:34:21.647863"
+    },
+    "versions_pair_end_multiple_stub": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-24T14:34:48.03415"
+    },
+    "sarscov2 - single_end stub": {
+        "content": [
+            [
+                
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-23T09:34:16.444372"
+    },
     "versions_single_end": {
         "content": [
             [
@@ -23,6 +62,42 @@
         },
         "timestamp": "2024-02-06T17:10:56.121713"
     },
+    "versions_pair_end_stub": {
+        "content": [
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-23T08:38:01.276656"
+    },
+    "versions_pair_end stub": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-24T14:34:36.570127"
+    },
+    "versions_single_end_stub": {
+        "content": [
+            [
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-04-24T14:28:01.864343"
+    },
     "versions_single_end_lib_type_a": {
         "content": [
             [
diff --git a/nextflow.config b/nextflow.config
index 871f543c7..14127c38f 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -83,6 +83,7 @@ params {
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
     hgnc_ref                      = "${params.genomes_base}/hgnc/hgnc_complete_set.txt"
     hgnc_date                     = "${params.genomes_base}/hgnc/HGNC-DB-timestamp.txt"
+    salmon_index                  = "${params.genomes_base}/salmon/salmon"
     starfusion_ref                = "${params.genomes_base}/starfusion/ctat_genome_lib_build_dir"
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 63c90c19d..69270631f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -200,6 +200,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Use QIAGEN instead of SANGER to download COSMIC database"
                 },
+                "salmon_index": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to salmon index"
+                },
                 "starfusion": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index db28c2a8a..038f460ae 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -25,8 +25,8 @@ include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genome
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
 include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
 include { SALMON_INDEX }                    from '../modules/nf-core/salmon/index/main'
-include { GFFREAD                                      } from '../modules/local/gffread'
-include { GUNZIP } from '../modules/nf-core/gunzip/main'
+include { GFFREAD }                         from '../modules/nf-core/gffread/main'
+
 /*
 ========================================================================================
     RUN MAIN WORKFLOW
@@ -38,54 +38,47 @@ workflow BUILD_REFERENCES {
     def fake_meta = [:]
     fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
     ENSEMBL_DOWNLOAD( params.ensembl_version, params.genome, fake_meta )
-    // HGNC_DOWNLOAD( )
-    GUNZIP(ENSEMBL_DOWNLOAD.out.primary_assembly)
-
-
-    SAMTOOLS_FAIDX(GUNZIP.out.gunzip, [[],[]])
-    // GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.fasta)
-
-    // RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
-    // CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
-
-    // GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
-
-    ch_fasta_fai =(GUNZIP.out.gunzip).join(SAMTOOLS_FAIDX.out.fai)
-    GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ch_fasta_fai)
-    SALMON_INDEX(GUNZIP.out.gunzip.map { meta, fasta -> [ fasta ] }, GFFREAD.out.tr_fasta)
-
-    // RSEM_PREPAREREFERENCE(ENSEMBL_DOWNLOAD.out.fasta.map { meta, fasta -> [ fasta ] }, ENSEMBL_DOWNLOAD.out.gtf.map { meta, gtf -> [ gtf ] })
-
-
-    // if (params.starindex || params.all || params.starfusion || params.arriba) {
-    //     STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
-    // }
-
-    // if (params.arriba || params.all) {
-    //     ARRIBA_DOWNLOAD()
-    // }
-
-    // if (params.fusioncatcher || params.all) {
-    //     FUSIONCATCHER_DOWNLOAD()
-    // }
-
-    // if (params.starfusion || params.all) {
-    //     if (params.starfusion_build){
-    //         STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
-    //     } else {
-    //         STARFUSION_DOWNLOAD()
-    //     }
-    // }
-
-    // if (params.starfusion_build){
-    //     GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
-    // } else {
-    //     GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
-    // }
-
-    // if (params.fusionreport || params.all) {
-    //     FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
-    // }
+    HGNC_DOWNLOAD( )
+    SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.primary_assembly, [[],[]])
+    GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.primary_assembly)
+
+    RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
+    CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
+
+    GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
+
+    GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ENSEMBL_DOWNLOAD.out.primary_assembly.map { meta, fasta -> [ fasta ] })
+    SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+
+    if (params.starindex || params.all || params.starfusion || params.arriba) {
+        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+    }
+
+    if (params.arriba || params.all) {
+        ARRIBA_DOWNLOAD()
+    }
+
+    if (params.fusioncatcher || params.all) {
+        FUSIONCATCHER_DOWNLOAD()
+    }
+
+    if (params.starfusion || params.all) {
+        if (params.starfusion_build){
+            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
+        } else {
+            STARFUSION_DOWNLOAD()
+        }
+    }
+
+    if (params.starfusion_build){
+        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
+    } else {
+        GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
+    }
+
+    if (params.fusionreport || params.all) {
+        FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
+    }
 
 }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 9837c7495..72c14ea1e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -6,6 +6,7 @@
 
 include { FASTQC                 } from '../modules/nf-core/fastqc/main'
 include { MULTIQC                } from '../modules/nf-core/multiqc/main'
+include { SALMON_QUANT           } from '../modules/nf-core/salmon/quant/main'
 include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
@@ -25,6 +26,7 @@ ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }
 ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
 ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
 ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
+ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
 ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
 ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
 
@@ -113,6 +115,7 @@ workflow RNAFUSION {
     .set { ch_cat_fastq }
     ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
 
+
     //
     // MODULE: Run FastQC
     //
@@ -129,6 +132,10 @@ workflow RNAFUSION {
     ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
+
+    SALMON_QUANT( ch_reads_all, ch_salmon_index, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+
+
     //
     // SUBWORKFLOW:  Run STAR alignment and Arriba
     //

From 0999af252b16a8714a78b5d650d103f688a0e967 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 13:27:15 +0200
Subject: [PATCH 019/887] cleanup

---
 subworkflows/local/qc_workflow.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index 0bc37a601..d7958fecc 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -5,7 +5,7 @@
 include { PICARD_COLLECTRNASEQMETRICS }                from '../../modules/local/picard/collectrnaseqmetrics/main'
 include { GATK4_MARKDUPLICATES }                       from '../../modules/nf-core/gatk4/markduplicates/main'
 include { PICARD_COLLECTINSERTSIZEMETRICS }            from '../../modules/nf-core/picard/collectinsertsizemetrics/main'
- include { SALMON_QUANT }                              from '../../modules/nf-core/salmon/quant/main'
+
 workflow QC_WORKFLOW {
     take:
         ch_reads_all
@@ -32,7 +32,6 @@ workflow QC_WORKFLOW {
         ch_versions = ch_versions.mix(PICARD_COLLECTINSERTSIZEMETRICS.out.versions)
         ch_insertsize_metrics = Channel.empty().mix(PICARD_COLLECTINSERTSIZEMETRICS.out.metrics)
 
-        SALMON_QUANT(ch_reads_all, salmon_index, ch_chrgtf.map { meta, gtf -> [ gtf ]}, transcript_fasta, true, '')
 
 
     emit:

From 3f47ecdc693f1e47185be055a02558fceb7eae96 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 16:18:28 +0200
Subject: [PATCH 020/887] remove local gffread

---
 modules/local/gffread.nf | 50 ----------------------------------------
 1 file changed, 50 deletions(-)
 delete mode 100644 modules/local/gffread.nf

diff --git a/modules/local/gffread.nf b/modules/local/gffread.nf
deleted file mode 100644
index 98863efa5..000000000
--- a/modules/local/gffread.nf
+++ /dev/null
@@ -1,50 +0,0 @@
-process GFFREAD {
-    tag "$gff"
-    label 'process_low'
-
-    conda "bioconda::gffread=0.12.1"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0' :
-        'biocontainers/gffread:0.12.1--h8b12597_0' }"
-
-    input:
-    tuple val(meta), path(gff)
-    tuple val(meta2), path(fasta), path(fai)
-
-    output:
-    path "*_gffread_output.gtf", emit: gtf,        optional: true
-    path "*_gffread_output.fa"  , emit: tr_fasta,   optional: true
-    path "versions.yml" , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args   = task.ext.args   ?: ''
-    def genome = fasta ? "-g ${fasta}" : ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def outp   = args.contains("-w") ? "-w ${prefix}_gffread_output.fa" : "-o ${prefix}_gffread_output.gtf"
-
-    """
-    gffread \\
-        $gff \\
-        $genome \\
-        $outp
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gffread: \$(gffread --version 2>&1)
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}_gffread"
-    """
-    touch ${prefix}_gffread_output.gtf
-    touch ${prefix}_gffread_output.fa
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gffread: \$(gffread --version 2>&1)
-    END_VERSIONS
-    """
-}

From 7f1065afe127e69db33fe13a34179e263c14235c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 16:21:53 +0200
Subject: [PATCH 021/887] use primary assembly as main fasta and gtf instead of
 chr

---
 workflows/build_references.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 038f460ae..5b523db3f 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -51,7 +51,7 @@ workflow BUILD_REFERENCES {
     SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
 
     if (params.starindex || params.all || params.starfusion || params.arriba) {
-        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.gtf )
+        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
     }
 
     if (params.arriba || params.all) {
@@ -64,16 +64,16 @@ workflow BUILD_REFERENCES {
 
     if (params.starfusion || params.all) {
         if (params.starfusion_build){
-            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.fasta, ENSEMBL_DOWNLOAD.out.chrgtf )
+            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
         } else {
             STARFUSION_DOWNLOAD()
         }
     }
 
     if (params.starfusion_build){
-        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.chrgtf)
+        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.gtf)
     } else {
-        GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.chrgtf)
+        GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.gtf)
     }
 
     if (params.fusionreport || params.all) {

From e98df822b6b970ea7f964156f6ccd7adbcfbc5cd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 16:24:05 +0200
Subject: [PATCH 022/887] fix channel io

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 72c14ea1e..45fd77eb0 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -133,7 +133,7 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
 
-    SALMON_QUANT( ch_reads_all, ch_salmon_index, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+    SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
 
 
     //

From 8b599637cf4e9dd8a2300d166d1ae29db0d3f054 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 May 2024 17:19:29 +0200
Subject: [PATCH 023/887] update changelog and output

---
 CHANGELOG.md   | 18 +++++++++++-------
 docs/output.md | 13 +++++++++++++
 2 files changed, 24 insertions(+), 7 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 18fabee33..5effdca59 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Added
 
+- Normalized gene expression calculated [#488](https://github.com/nf-core/rnafusion/pull/488)
+- Primary assembly now used as main reference genome FASTA file, as recommended by the STAR manual [#488](https://github.com/nf-core/rnafusion/pull/488)
+- Use of only ensembl GTF file, not chr.gtf file as GTF reference file [#488](https://github.com/nf-core/rnafusion/pull/488)
+
 ### Changed
 
 ### Fixed
@@ -24,12 +28,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Fixed
 
-- fix bug when using parameter "whitelist" [#466](https://github.com/nf-core/rnafusion/pull/466)
-- fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report [#458](https://github.com/nf-core/rnafusion/pull/458)
-- fix VCF_COLLECT when fusioninspector output is empty but fusionreport is not [#465](https://github.com/nf-core/rnafusion/pull/465)
-- fix VCF_COLLECT bug [#481](https://github.com/nf-core/rnafusion/pull/481)
-- fix conda package for starfusion/detect[#482](https://github.com/nf-core/rnafusion/pull/482)
-- fix logical gate so when stringtie should run but not starfusion, starfusion will not run[#482](https://github.com/nf-core/rnafusion/pull/482)
+- Fix bug when using parameter "whitelist" [#466](https://github.com/nf-core/rnafusion/pull/466)
+- Fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report [#458](https://github.com/nf-core/rnafusion/pull/458)
+- Fix VCF_COLLECT when fusioninspector output is empty but fusionreport is not [#465](https://github.com/nf-core/rnafusion/pull/465)
+- Fix VCF_COLLECT bug [#481](https://github.com/nf-core/rnafusion/pull/481)
+- Fix conda package for starfusion/detect[#482](https://github.com/nf-core/rnafusion/pull/482)
+- Fix logical gate so when stringtie should run but not starfusion, starfusion will not run[#482](https://github.com/nf-core/rnafusion/pull/482)
 
 ### Removed
 
@@ -39,7 +43,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Changed
 
-- python3 explicit in vcf_collect [#452](https://github.com/nf-core/rnafusion/pull/452)
+- Python3 explicit in vcf_collect [#452](https://github.com/nf-core/rnafusion/pull/452)
 
 ### Fixed
 
diff --git a/docs/output.md b/docs/output.md
index dede2c87b..128a2f365 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -23,6 +23,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 - [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report for FusionInspector fusions
 - [Picard](#picard) - Collect QC metrics
 - [FastQC](#fastqc) - Raw read quality control
+- [Salmon](#salmon) - Normalized gene expression calculation
 - [MultiQC](#multiqc) - Aggregate reports describing QC results from the whole pipeline
 - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
@@ -234,6 +235,18 @@ $$
 
 All tools have the same weight.
 
+### Salmon
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `salmon`
+  - `<sample>`
+
+</details>
+
+Folder containing the quantification results
+
 ### Kallisto
 
 <details markdown="1">

From 83e820a6524e3ac709f921ef922a25830296766e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 May 2024 10:45:34 +0200
Subject: [PATCH 024/887] Update conf/modules.config
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Co-authored-by: Lucía Peña-Pérez <lucia.pena.perez@scilifelab.se>
---
 conf/modules.config | 4 ++++
 1 file changed, 4 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 696889c8e..a1fe17026 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -190,6 +190,10 @@ process {
     }
 
     withName: 'SALMON_QUANT' {
+        ext.args = { [
+            '--gcBias',
+            '--validateMappings'
+            ].join(' ') }
         publishDir = [
             path: { "${params.outdir}/salmon" },
             mode: params.publish_dir_mode,

From 7a2aca5d42e0df71d2ffa65f8160d10ced94cb44 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 May 2024 11:48:17 +0200
Subject: [PATCH 025/887] update modules and use rrnatranscript from nf-core

---
 modules.json                                  | 13 ++-
 .../agat/convertspgff2tsv/environment.yml     |  2 +-
 modules/nf-core/agat/convertspgff2tsv/main.nf |  8 +-
 .../nf-core/agat/convertspgff2tsv/meta.yml    |  2 +
 .../agat/convertspgff2tsv/tests/main.nf.test  |  2 -
 .../convertspgff2tsv/tests/main.nf.test.snap  | 22 +++--
 modules/nf-core/cat/fastq/tests/main.nf.test  |  2 +
 .../tests/main.nf.test                        | 56 ++++++++++++
 .../tests/main.nf.test.snap                   | 68 +++++++++++++++
 .../createsequencedictionary/tests/tags.yml   |  2 +
 .../nf-core/rrnatranscripts/environment.yml   |  9 ++
 modules/nf-core/rrnatranscripts/main.nf       | 43 ++++++++++
 modules/nf-core/rrnatranscripts/meta.yml      | 31 +++++++
 .../templates/get_rrna_transcripts.py         | 85 +++++++++++++++++++
 .../rrnatranscripts/tests/main.nf.test        | 53 ++++++++++++
 .../rrnatranscripts/tests/main.nf.test.snap   | 40 +++++++++
 .../nf-core/rrnatranscripts/tests/tags.yml    |  2 +
 modules/nf-core/ucsc/gtftogenepred/main.nf    |  2 +-
 workflows/build_references.nf                 | 11 +--
 19 files changed, 429 insertions(+), 24 deletions(-)
 create mode 100644 modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test
 create mode 100644 modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml
 create mode 100644 modules/nf-core/rrnatranscripts/environment.yml
 create mode 100644 modules/nf-core/rrnatranscripts/main.nf
 create mode 100644 modules/nf-core/rrnatranscripts/meta.yml
 create mode 100644 modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
 create mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test
 create mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/rrnatranscripts/tests/tags.yml

diff --git a/modules.json b/modules.json
index eb75243ad..d8fe40315 100644
--- a/modules.json
+++ b/modules.json
@@ -7,7 +7,7 @@
                 "nf-core": {
                     "agat/convertspgff2tsv": {
                         "branch": "master",
-                        "git_sha": "9198082dcec6d8374c0bf3d7e38dd1f79d4601a7",
+                        "git_sha": "b1d41826971b91f9649b46a8f3ccb226ee150c85",
                         "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
@@ -32,7 +32,7 @@
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "0997b47c93c06b49aa7b3fefda87e728312cf2ca",
+                        "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e",
                         "installed_by": ["modules"]
                     },
                     "fastp": {
@@ -52,7 +52,7 @@
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
-                        "git_sha": "d742e3143f2ccb8853c29b35cfcf50b5e5026980",
+                        "git_sha": "e6fe277739f5894711405af3e717b2470bd956b5",
                         "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
@@ -80,6 +80,11 @@
                         "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
                         "installed_by": ["modules"]
                     },
+                    "rrnatranscripts": {
+                        "branch": "master",
+                        "git_sha": "7b17de553ee27a17478f6bf9df15acd26b1ef377",
+                        "installed_by": ["modules"]
+                    },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "f153f1f10e1083c49935565844cccb7453021682",
@@ -122,7 +127,7 @@
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "acb0880789a6ebc2168d3b2d3d42b5bce6a62431",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/agat/convertspgff2tsv/environment.yml b/modules/nf-core/agat/convertspgff2tsv/environment.yml
index b5fdf3dbb..690f50ae5 100644
--- a/modules/nf-core/agat/convertspgff2tsv/environment.yml
+++ b/modules/nf-core/agat/convertspgff2tsv/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::agat=1.2.0
+  - bioconda::agat=1.4.0
diff --git a/modules/nf-core/agat/convertspgff2tsv/main.nf b/modules/nf-core/agat/convertspgff2tsv/main.nf
index 5cded33ef..a6f73b6c1 100644
--- a/modules/nf-core/agat/convertspgff2tsv/main.nf
+++ b/modules/nf-core/agat/convertspgff2tsv/main.nf
@@ -4,8 +4,8 @@ process AGAT_CONVERTSPGFF2TSV {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/agat:1.2.0--pl5321hdfd78af_0' :
-        'biocontainers/agat:1.2.0--pl5321hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/agat:1.4.0--pl5321hdfd78af_0' :
+        'biocontainers/agat:1.4.0--pl5321hdfd78af_0' }"
 
     input:
     tuple val(meta), path(gff)
@@ -28,7 +28,7 @@ process AGAT_CONVERTSPGFF2TSV {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        agat: \$(agat_convert_sp_gff2tsv.pl --help | sed '3!d; s/.*v//' | sed 's/ .*//')
+        agat: \$(agat_convert_sp_gff2tsv.pl --help | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p')
     END_VERSIONS
     """
 
@@ -40,7 +40,7 @@ process AGAT_CONVERTSPGFF2TSV {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        agat: \$(agat_convert_sp_gff2tsv.pl --help | sed '3!d; s/.*v//' | sed 's/ .*//')
+        agat: \$(agat_convert_sp_gff2tsv.pl --help | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p')
     END_VERSIONS
     """
 }
diff --git a/modules/nf-core/agat/convertspgff2tsv/meta.yml b/modules/nf-core/agat/convertspgff2tsv/meta.yml
index f5865dfe7..83078787b 100644
--- a/modules/nf-core/agat/convertspgff2tsv/meta.yml
+++ b/modules/nf-core/agat/convertspgff2tsv/meta.yml
@@ -36,3 +36,5 @@ output:
       pattern: "versions.yml"
 authors:
   - "@rannick"
+maintainers:
+  - "@gallvp"
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
index 41239a588..4898a4a9b 100644
--- a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
+++ b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
@@ -14,8 +14,6 @@ nextflow_process {
         when {
             process {
                 """
-                // TODO nf-core: define inputs of the process here. Example:
-
                 input[0] = [
                             [ id: 'test' ], // meta map
                             file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
index cf21cd659..71ed6205c 100644
--- a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
+++ b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
@@ -3,10 +3,14 @@
         "content": [
             [
                 "test.tsv",
-                "versions.yml:md5,07ae8e1f648a67237a7efeafb551f01c"
+                "versions.yml:md5,b81565a6ff8911848806128b3bec8508"
             ]
         ],
-        "timestamp": "2023-12-24T23:55:30.786170144"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T10:06:55.853319"
     },
     "sarscov2 - genome [gff3]": {
         "content": [
@@ -16,25 +20,29 @@
                         {
                             "id": "test"
                         },
-                        "test.tsv:md5,e565b8ee5bc69b0625046a8036fda552"
+                        "test.tsv:md5,8373d2035689d23694f87606116cdccd"
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,07ae8e1f648a67237a7efeafb551f01c"
+                    "versions.yml:md5,b81565a6ff8911848806128b3bec8508"
                 ],
                 "tsv": [
                     [
                         {
                             "id": "test"
                         },
-                        "test.tsv:md5,e565b8ee5bc69b0625046a8036fda552"
+                        "test.tsv:md5,8373d2035689d23694f87606116cdccd"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,07ae8e1f648a67237a7efeafb551f01c"
+                    "versions.yml:md5,b81565a6ff8911848806128b3bec8508"
                 ]
             }
         ],
-        "timestamp": "2023-12-24T23:55:20.642366893"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-05-08T10:06:51.415395"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test b/modules/nf-core/cat/fastq/tests/main.nf.test
index dab2e14c0..a71dcb8df 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test
@@ -1,3 +1,5 @@
+// NOTE The version snaps may not be consistant
+// https://github.com/nf-core/modules/pull/4087#issuecomment-1767948035
 nextflow_process {
 
     name "Test Process CAT_FASTQ"
diff --git a/modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test b/modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test
new file mode 100644
index 000000000..a8a9c6d2e
--- /dev/null
+++ b/modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test
@@ -0,0 +1,56 @@
+nextflow_process {
+
+    name "Test Process GATK4_CREATESEQUENCEDICTIONARY"
+    script "../main.nf"
+    process "GATK4_CREATESEQUENCEDICTIONARY"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "gatk4"
+    tag "gatk4/createsequencedictionary"
+
+    test("sarscov2 - fasta") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                    ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 - fasta - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                    ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test.snap b/modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test.snap
new file mode 100644
index 000000000..16735f954
--- /dev/null
+++ b/modules/nf-core/gatk4/createsequencedictionary/tests/main.nf.test.snap
@@ -0,0 +1,68 @@
+{
+    "sarscov2 - fasta - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.dict:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,e60dd34a71fc2029d81dc67ccb5d6be6"
+                ],
+                "dict": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.dict:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e60dd34a71fc2029d81dc67ccb5d6be6"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-05-16T10:16:16.34453"
+    },
+    "sarscov2 - fasta": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.dict:md5,7362679f176e0f52add03c08f457f646"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,e60dd34a71fc2029d81dc67ccb5d6be6"
+                ],
+                "dict": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.dict:md5,7362679f176e0f52add03c08f457f646"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e60dd34a71fc2029d81dc67ccb5d6be6"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.0"
+        },
+        "timestamp": "2024-05-16T13:58:25.822068"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml b/modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml
new file mode 100644
index 000000000..035c5e4c7
--- /dev/null
+++ b/modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml
@@ -0,0 +1,2 @@
+gatk4/createsequencedictionary:
+  - "modules/nf-core/gatk4/createsequencedictionary/**"
diff --git a/modules/nf-core/rrnatranscripts/environment.yml b/modules/nf-core/rrnatranscripts/environment.yml
new file mode 100644
index 000000000..e812273bf
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/environment.yml
@@ -0,0 +1,9 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+name: "rrnatranscripts"
+channels:
+  - conda-forge
+  - bioconda
+  - defaults
+dependencies:
+  - python=3.12.2
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
new file mode 100644
index 000000000..50843c768
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/main.nf
@@ -0,0 +1,43 @@
+process RRNATRANSCRIPTS {
+    tag '$rrna'
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/python:3.12' :
+        'biocontainers/python:3.12' }"
+
+    input:
+    path(gtf)
+
+    output:
+    path("*rrna_intervals.gtf") , emit: rrna_gtf, optional: true
+    path "versions.yml"         , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def prefix = task.ext.prefix ?: "${gtf.baseName}"
+    """
+    grep -E '^#|rRNA' genome.gtf > ${prefix}_rrna_intervals.gtf || true
+    if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
+        rm ${prefix}_rrna_intervals.gtf
+    fi
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | sed -e "s/Python //g")
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${gtf.baseName}"
+    """
+    touch ${prefix}_rrna_intervals.gtf
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | sed -e "s/Python //g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/rrnatranscripts/meta.yml b/modules/nf-core/rrnatranscripts/meta.yml
new file mode 100644
index 000000000..145506011
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/meta.yml
@@ -0,0 +1,31 @@
+name: rrnatranscripts
+description: Ribosomal RNA extraction from a GTF file.
+keywords:
+  - ribosomal
+  - rna
+  - genomics
+tools:
+  - rrnatranscripts:
+      description: |
+        Extraction of ribosomal RNA
+      homepage: https://github.com/nf-core/rnafusion
+      licence: ["GPL-3.0-or-later"]
+input:
+  - gtf:
+      type: file
+      description: GTF file
+      pattern: "*.{gtf}"
+output:
+  #
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - rrna_gtf:
+      type: file
+      description: GTF file with ribosomal RNA only
+      pattern: "*.{gtf}"
+authors:
+  - "@rannick"
+maintainers:
+  - "@rannick"
diff --git a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
new file mode 100644
index 000000000..2e320e7bd
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
@@ -0,0 +1,85 @@
+#!/usr/bin/env python3
+
+import logging
+import platform
+import sys
+from pathlib import Path
+
+# Configure logging
+logging.basicConfig(format="%(name)s - %(asctime)s %(levelname)s: %(message)s")
+logger = logging.getLogger(__name__)
+logger.setLevel(logging.INFO)
+
+def get_rrna_intervals(gtf: str, rrna_transcripts: str):
+    """
+    Get lines containing ``#`` or ``gene_type rRNA`` or ```` or ``gene_type rRNA_pseudogene`` or ``gene_type MT_rRNA``
+    Create output file
+
+    Args:
+        file_in (pathlib.Path): The given GTF file.
+        file_out (pathlib.Path): Where the ribosomal RNA GTF file should
+            be created; always in GTF format.
+    """
+    patterns = {
+        "#",
+        'transcript_biotype "Mt_rRNA"',
+        'transcript_biotype "rRNA"',
+        'transcript_biotype "rRNA_pseudogene"',
+    }
+    line_starts = {"MT", "1", "2", "3", "4", "5", "6", "7", "8", "9"}
+    out_lines = []
+    path_gtf = Path(gtf)
+    path_rrna_transcripts = Path(rrna_transcripts)
+    if not path_gtf.is_file():
+        logger.error(f"The given input file {gtf} was not found!")
+        sys.exit(2)
+    with path_gtf.open() as f:
+        data = f.readlines()
+        for line in data:
+            for pattern in patterns:
+                if pattern in line:
+                    for line_start in line_starts:
+                        if line.startswith(line_start):
+                            out_lines.append(line)
+    if out_lines != []:
+        with path_rrna_transcripts.open(mode="w") as out_file:
+            out_file.writelines(out_lines)
+
+
+def format_yaml_like(data: dict, indent: int = 0) -> str:
+    """Formats a dictionary to a YAML-like string.
+
+    Args:
+        data (dict): The dictionary to format.
+        indent (int): The current indentation level.
+
+    Returns:
+        str: A string formatted as YAML.
+    """
+    yaml_str = ""
+    for key, value in data.items():
+        spaces = "  " * indent
+        if isinstance(value, dict):
+            yaml_str += f"{spaces}{key}:\\n{format_yaml_like(value, indent + 1)}"
+        else:
+            yaml_str += f"{spaces}{key}: {value}\\n"
+    return yaml_str
+
+
+if __name__ == "__main__":
+    if '${task.ext.prefix}' != "null":
+        prefix = "${task.ext.prefix}."
+    else:
+        prefix = "${task.ext.gtf}."
+
+    if not get_rrna_intervals('$gtf', f"{prefix}_rrna_intervals.gtf"):
+        logging.error("Failed to extract rrna transcipts.")
+
+
+
+    # Write the versions
+    versions_this_module = {}
+    versions_this_module["${task.process}"] = {"python": platform.python_version()}
+    with open("versions.yml", "w") as f:
+        f.write(format_yaml_like(versions_this_module))
+
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test b/modules/nf-core/rrnatranscripts/tests/main.nf.test
new file mode 100644
index 000000000..1c29522ad
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/tests/main.nf.test
@@ -0,0 +1,53 @@
+nextflow_process {
+
+    name "Test Process RRNATRANSCRIPTS"
+    script "../main.nf"
+    process "RRNATRANSCRIPTS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "rrnatranscripts"
+
+    test("homo_sapiens - gtf") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                            file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
+                ]                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - gtf - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
+                ]                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap b/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
new file mode 100644
index 000000000..a190ddcbf
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
@@ -0,0 +1,40 @@
+{
+    "homo_sapiens - gtf": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
+                ],
+                "rrna_gtf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
+                ]
+            }
+        ],
+        "timestamp": "2024-05-21T13:55:44.831609"
+    },
+    "homo_sapiens - gtf - stub": {
+        "content": [
+            {
+                "0": [
+                    "genome_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "1": [
+                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
+                ],
+                "rrna_gtf": [
+                    "genome_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "versions": [
+                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
+                ]
+            }
+        ],
+        "timestamp": "2024-05-21T13:55:56.134136"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/rrnatranscripts/tests/tags.yml b/modules/nf-core/rrnatranscripts/tests/tags.yml
new file mode 100644
index 000000000..ade15226a
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/tests/tags.yml
@@ -0,0 +1,2 @@
+rrnatranscripts:
+  - "modules/nf-core/rrnatranscripts/**"
diff --git a/modules/nf-core/ucsc/gtftogenepred/main.nf b/modules/nf-core/ucsc/gtftogenepred/main.nf
index 88aace26b..afbb5f3f9 100644
--- a/modules/nf-core/ucsc/gtftogenepred/main.nf
+++ b/modules/nf-core/ucsc/gtftogenepred/main.nf
@@ -1,5 +1,5 @@
 process UCSC_GTFTOGENEPRED {
-    tag '${meta.id}'
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index b1ea6d70c..f492de601 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -10,7 +10,7 @@ include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/
 include { HGNC_DOWNLOAD }                   from '../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../modules/local/starfusion/build/main'
 include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
-include { RRNA_TRANSCRIPTS }                from '../modules/local/rrnatranscripts/main'
+
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
@@ -18,9 +18,10 @@ include { RRNA_TRANSCRIPTS }                from '../modules/local/rrnatranscrip
 */
 
 include { ARRIBA_DOWNLOAD }                 from '../modules/nf-core/arriba/download/main'
-include { BEDOPS_CONVERT2BED } from '../modules/nf-core/bedops/convert2bed/main'
+include { BEDOPS_CONVERT2BED }              from '../modules/nf-core/bedops/convert2bed/main'
 include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
+include { RRNATRANSCRIPTS }                 from '../modules/nf-core/rrnatranscripts/main'
 include { SAMTOOLS_FAIDX }                  from '../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genomegenerate/main'
 include { UCSC_GTFTOGENEPRED }              from '../modules/nf-core/ucsc/gtftogenepred/main'
@@ -36,14 +37,14 @@ workflow BUILD_REFERENCES {
     def fake_meta = [:]
     fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
     ENSEMBL_DOWNLOAD( params.ensembl_version, params.genome, fake_meta )
-    HGNC_DOWNLOAD( )
+    HGNC_DOWNLOAD()
 
 
     SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.fasta, [[],[]])
     GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.fasta)
 
-    RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
-    BEDOPS_CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
+    RRNATRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf.map{ meta, gtf -> [ gtf ] })
+    BEDOPS_CONVERT2BED([fake_meta.mix(RRNATRANSCRIPTS.out.rrna_gtf)])
 
     GATK4_BEDTOINTERVALLIST(BEDOPS_CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
 

From c8b33090e8ca3364ac80e8f690594978fd8bbb29 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 May 2024 12:50:20 +0200
Subject: [PATCH 026/887] fix channel io

---
 workflows/build_references.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index f492de601..32bfdce53 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -44,8 +44,7 @@ workflow BUILD_REFERENCES {
     GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.fasta)
 
     RRNATRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf.map{ meta, gtf -> [ gtf ] })
-    BEDOPS_CONVERT2BED([fake_meta.mix(RRNATRANSCRIPTS.out.rrna_gtf)])
-
+    BEDOPS_CONVERT2BED(RRNATRANSCRIPTS.out.rrna_gtf.map{ it -> [[id:it.Name], it] })
     GATK4_BEDTOINTERVALLIST(BEDOPS_CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
 
 

From eeeb9aebacb895e6808f0eaf12765a80a35af79d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 May 2024 12:55:33 +0200
Subject: [PATCH 027/887] remove gunzip

---
 modules.json                                  | 111 +++++++++++++-----
 modules/nf-core/gunzip/environment.yml        |   7 --
 modules/nf-core/gunzip/main.nf                |  48 --------
 modules/nf-core/gunzip/meta.yml               |  39 ------
 modules/nf-core/gunzip/tests/main.nf.test     |  36 ------
 .../nf-core/gunzip/tests/main.nf.test.snap    |  31 -----
 modules/nf-core/gunzip/tests/tags.yml         |   2 -
 7 files changed, 79 insertions(+), 195 deletions(-)
 delete mode 100644 modules/nf-core/gunzip/environment.yml
 delete mode 100644 modules/nf-core/gunzip/main.nf
 delete mode 100644 modules/nf-core/gunzip/meta.yml
 delete mode 100644 modules/nf-core/gunzip/tests/main.nf.test
 delete mode 100644 modules/nf-core/gunzip/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/gunzip/tests/tags.yml

diff --git a/modules.json b/modules.json
index 4157b3177..fded9d460 100644
--- a/modules.json
+++ b/modules.json
@@ -8,122 +8,163 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba": {
                         "branch": "master",
                         "git_sha": "bbaeb3a3149def237b9d0acb9ab749ae98088e29",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "6c996d7fbe0816dcbb68ce587ad5f873313682a1",
-                        "installed_by": ["modules"]
-                    },
-                    "gunzip": {
-                        "branch": "master",
-                        "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "9f6b233518f7d9ecdcf24b798b7e491db5424273",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "0e98289b5bec6e3f8f588a8a9d05e8aacc1179a0",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "5756b12b1fab0c07f05b9ad2d5d5c262b1a2c1ef",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "b1b959609bda44341120aed1766329909f54b8d0",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -132,20 +173,26 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfvalidation_plugin": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml
deleted file mode 100644
index 25910b340..000000000
--- a/modules/nf-core/gunzip/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-name: gunzip
-channels:
-  - conda-forge
-  - bioconda
-  - defaults
-dependencies:
-  - conda-forge::sed=4.7
diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf
deleted file mode 100644
index 468a6f287..000000000
--- a/modules/nf-core/gunzip/main.nf
+++ /dev/null
@@ -1,48 +0,0 @@
-process GUNZIP {
-    tag "$archive"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
-        'nf-core/ubuntu:20.04' }"
-
-    input:
-    tuple val(meta), path(archive)
-
-    output:
-    tuple val(meta), path("$gunzip"), emit: gunzip
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    gunzip = archive.toString() - '.gz'
-    """
-    # Not calling gunzip itself because it creates files
-    # with the original group ownership rather than the
-    # default one for that user / the work directory
-    gzip \\
-        -cd \\
-        $args \\
-        $archive \\
-        > $gunzip
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    gunzip = archive.toString() - '.gz'
-    """
-    touch $gunzip
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml
deleted file mode 100644
index 231034f2f..000000000
--- a/modules/nf-core/gunzip/meta.yml
+++ /dev/null
@@ -1,39 +0,0 @@
-name: gunzip
-description: Compresses and decompresses files.
-keywords:
-  - gunzip
-  - compression
-  - decompression
-tools:
-  - gunzip:
-      description: |
-        gzip is a file format and a software application used for file compression and decompression.
-      documentation: https://www.gnu.org/software/gzip/manual/gzip.html
-      licence: ["GPL-3.0-or-later"]
-input:
-  - meta:
-      type: map
-      description: |
-        Optional groovy Map containing meta information
-        e.g. [ id:'test', single_end:false ]
-  - archive:
-      type: file
-      description: File to be compressed/uncompressed
-      pattern: "*.*"
-output:
-  - gunzip:
-      type: file
-      description: Compressed/uncompressed file
-      pattern: "*.*"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-authors:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@jfy133"
-maintainers:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@jfy133"
diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test
deleted file mode 100644
index 6406008ef..000000000
--- a/modules/nf-core/gunzip/tests/main.nf.test
+++ /dev/null
@@ -1,36 +0,0 @@
-nextflow_process {
-
-    name "Test Process GUNZIP"
-    script "../main.nf"
-    process "GUNZIP"
-    tag "gunzip"
-    tag "modules_nfcore"
-    tag "modules"
-
-    test("Should run without failures") {
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-            process {
-                """
-                input[0] = Channel.of([
-                        [],
-                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
-                    ]
-                )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-            { assert process.success },
-            { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap
deleted file mode 100644
index 720fd9ff4..000000000
--- a/modules/nf-core/gunzip/tests/main.nf.test.snap
+++ /dev/null
@@ -1,31 +0,0 @@
-{
-    "Should run without failures": {
-        "content": [
-            {
-                "0": [
-                    [
-                        [
-                            
-                        ],
-                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ],
-                "gunzip": [
-                    [
-                        [
-                            
-                        ],
-                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ]
-            }
-        ],
-        "timestamp": "2023-10-17T15:35:37.690477896"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml
deleted file mode 100644
index fd3f69154..000000000
--- a/modules/nf-core/gunzip/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-gunzip:
-  - modules/nf-core/gunzip/**

From 6d08a74d6f0fc6fc718e9ec96d14bf2200dc5c3c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 22 May 2024 13:03:31 +0200
Subject: [PATCH 028/887] prettier

---
 modules.json | 106 +++++++++++++--------------------------------------
 1 file changed, 27 insertions(+), 79 deletions(-)

diff --git a/modules.json b/modules.json
index fded9d460..7f4cf0efc 100644
--- a/modules.json
+++ b/modules.json
@@ -8,163 +8,117 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba": {
                         "branch": "master",
                         "git_sha": "bbaeb3a3149def237b9d0acb9ab749ae98088e29",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "02fd5bd7275abad27aad32d5c852e0a9b1b98882",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "6c996d7fbe0816dcbb68ce587ad5f873313682a1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "9f6b233518f7d9ecdcf24b798b7e491db5424273",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "0e98289b5bec6e3f8f588a8a9d05e8aacc1179a0",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "5756b12b1fab0c07f05b9ad2d5d5c262b1a2c1ef",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "b1b959609bda44341120aed1766329909f54b8d0",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -173,26 +127,20 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfvalidation_plugin": {
                         "branch": "master",
                         "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From 3332ebbc1dc877a875acf69f481627b595268ef8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 24 May 2024 15:32:43 +0200
Subject: [PATCH 029/887] WIP: restructure reference building

---
 conf/modules.config                    |   2 +-
 main.nf                                |  10 +-
 nextflow.config                        |   5 +-
 subworkflows/local/build_references.nf | 122 +++++++
 workflows/build_references.nf          |  89 -----
 workflows/rnafusion.nf                 | 436 +++++++++++++------------
 6 files changed, 347 insertions(+), 317 deletions(-)
 create mode 100644 subworkflows/local/build_references.nf
 delete mode 100644 workflows/build_references.nf

diff --git a/conf/modules.config b/conf/modules.config
index a1fe17026..44dec3397 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -59,7 +59,7 @@ process {
 
     withName: 'FASTQC' {
         ext.args = '--quiet'
-        ext.when         = { !params.skip_qc }
+        ext.when = { !params.skip_qc }
         publishDir = [
             path: { "${params.outdir}/fastqc" },
             mode: params.publish_dir_mode,
diff --git a/main.nf b/main.nf
index ac96ab669..4c945cf83 100644
--- a/main.nf
+++ b/main.nf
@@ -20,7 +20,6 @@ nextflow.enable.dsl = 2
 include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
-include { BUILD_REFERENCES        } from './workflows/build_references'
 include { RNAFUSION               } from './workflows/rnafusion'
 
 
@@ -50,12 +49,9 @@ workflow NFCORE_RNAFUSION {
     //
     // WORKFLOW: Run pipeline
     //
-    if (params.build_references) {
-        BUILD_REFERENCES ()
-    } else {
-        ch_samplesheet = Channel.value(file(params.input, checkIfExists: true))
-        RNAFUSION(ch_samplesheet)
-    }
+
+    ch_samplesheet = Channel.value(file(params.input, checkIfExists: true))
+    RNAFUSION(ch_samplesheet)
 
 }
 /*
diff --git a/nextflow.config b/nextflow.config
index 14127c38f..265c3c0b3 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -33,11 +33,10 @@ params {
     genomes                    = [:]
 
     // Genomes options
-    fasta          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"
-    fai            = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa.fai"
+    fasta          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa"
+    fai            = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa.fai"
     gtf            = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
     chrgtf         = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
-    transcript     = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz"
     refflat        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat"
     rrna_intervals = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list"
 
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
new file mode 100644
index 000000000..bb2acc4d3
--- /dev/null
+++ b/subworkflows/local/build_references.nf
@@ -0,0 +1,122 @@
+/*
+========================================================================================
+    IMPORT LOCAL MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+include { ARRIBA_DOWNLOAD }                 from '../../modules/local/arriba/download/main'
+include { ENSEMBL_DOWNLOAD }                from '../../modules/local/ensembl/main'
+include { FUSIONCATCHER_DOWNLOAD }          from '../../modules/local/fusioncatcher/download/main'
+include { FUSIONREPORT_DOWNLOAD }           from '../../modules/local/fusionreport/download/main'
+include { HGNC_DOWNLOAD }                   from '../../modules/local/hgnc/main'
+include { STARFUSION_BUILD }                from '../../modules/local/starfusion/build/main'
+include { STARFUSION_DOWNLOAD }             from '../../modules/local/starfusion/download/main'
+include { GTF_TO_REFFLAT }                  from '../../modules/local/uscs/custom_gtftogenepred/main'
+include { RRNA_TRANSCRIPTS }                from '../../modules/local/rrnatranscripts/main'
+include { CONVERT2BED }                     from '../../modules/local/convert2bed/main'
+/*
+========================================================================================
+    IMPORT NF-CORE MODULES/SUBWORKFLOWS
+========================================================================================
+*/
+
+include { SAMTOOLS_FAIDX }                  from '../../modules/nf-core/samtools/faidx/main'
+include { STAR_GENOMEGENERATE }             from '../../modules/nf-core/star/genomegenerate/main'
+include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../modules/nf-core/gatk4/createsequencedictionary/main'
+include { GATK4_BEDTOINTERVALLIST }         from '../../modules/nf-core/gatk4/bedtointervallist/main'
+include { SALMON_INDEX }                    from '../../modules/nf-core/salmon/index/main'
+include { GFFREAD }                         from '../../modules/nf-core/gffread/main'
+
+/*
+========================================================================================
+    RUN MAIN WORKFLOW
+========================================================================================
+*/
+
+workflow BUILD_REFERENCES {
+
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    def fake_meta = [:]
+    fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
+
+    if (!file(params.fasta).exists() || file(params.fasta).isEmpty() ||
+                    !file(params.chrgtf).exists() || file(params.chrgtf).isEmpty() ||
+                    !file(params.gtf).exists() || file(params.gtf).isEmpty()){
+        ENSEMBL_DOWNLOAD(params.ensembl_version, params.genome, fake_meta)}
+    ch_fasta = {(!file(params.fasta).exists() || file(params.fasta).isEmpty()) ? ENSEMBL_DOWNLOAD.out.primary_assembly : Channel.fromPath(params.fasta).map { that -> [[id:that.Name], that] }.collect()}
+
+    if (!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
+                    !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()){
+        HGNC_DOWNLOAD( )}
+
+    if (!file(params.fai).exists() || file(params.fai).isEmpty(){
+        SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.primary_assembly, [[],[]])}
+
+    if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty(){
+        GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.primary_assembly)
+        RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
+        CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
+        GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
+        }
+
+    if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty(){ // add condition for qc, check that dirs can also be checked with isEmpty()
+        GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ENSEMBL_DOWNLOAD.out.primary_assembly.map { meta, fasta -> [ fasta ] })
+        SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+        }
+
+
+    if ((params.starindex || params.all || params.starfusion || params.arriba) &&
+            (!params.starindex_ref.exits() || params.starindex_ref.isEmpty())
+            ) {
+        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
+    }
+    ch_starindex_ref = ...
+
+    // if (params.arriba || params.all) {
+    //     ARRIBA_DOWNLOAD()
+    // }
+
+    // if (params.fusioncatcher || params.all) {
+    //     FUSIONCATCHER_DOWNLOAD()
+    // }
+
+    // if (params.starfusion || params.all) {
+    //     if (params.starfusion_build){
+    //         STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
+    //     } else {
+    //         STARFUSION_DOWNLOAD()
+    //     }
+    // }
+
+    // if (params.starfusion_build){
+    //     GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.gtf)
+    // } else {
+    //     GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.gtf)
+    // }
+
+    // if (params.fusionreport || params.all) {
+    //     FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
+    // }
+
+    emit:
+    ch_fasta
+    ch_chrgtf = {(!file(params.chrgtf).exists() || file(params.chrgtf).isEmpty()) ? ENSEMBL_DOWNLOAD.out.chrgtf : Channel.fromPath(params.chrgtf).map { that -> [[id:that.Name], that] }.collect()}
+    ch_gtf = {(!file(params.gtf).exists() || file(params.gtf).isEmpty()) ? ENSEMBL_DOWNLOAD.out.gtf : Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }.collect()}
+    ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
+    ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
+    ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
+    ch_rrna_interval = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
+    ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
+
+
+}
+
+/*
+========================================================================================
+    THE END
+========================================================================================
+*/
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
deleted file mode 100644
index 5b523db3f..000000000
--- a/workflows/build_references.nf
+++ /dev/null
@@ -1,89 +0,0 @@
-/*
-========================================================================================
-    IMPORT LOCAL MODULES/SUBWORKFLOWS
-========================================================================================
-*/
-
-include { ARRIBA_DOWNLOAD }                 from '../modules/local/arriba/download/main'
-include { ENSEMBL_DOWNLOAD }                from '../modules/local/ensembl/main'
-include { FUSIONCATCHER_DOWNLOAD }          from '../modules/local/fusioncatcher/download/main'
-include { FUSIONREPORT_DOWNLOAD }           from '../modules/local/fusionreport/download/main'
-include { HGNC_DOWNLOAD }                   from '../modules/local/hgnc/main'
-include { STARFUSION_BUILD }                from '../modules/local/starfusion/build/main'
-include { STARFUSION_DOWNLOAD }             from '../modules/local/starfusion/download/main'
-include { GTF_TO_REFFLAT }                  from '../modules/local/uscs/custom_gtftogenepred/main'
-include { RRNA_TRANSCRIPTS }                from '../modules/local/rrnatranscripts/main'
-include { CONVERT2BED }                     from '../modules/local/convert2bed/main'
-/*
-========================================================================================
-    IMPORT NF-CORE MODULES/SUBWORKFLOWS
-========================================================================================
-*/
-
-include { SAMTOOLS_FAIDX }                  from '../modules/nf-core/samtools/faidx/main'
-include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genomegenerate/main'
-include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
-include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
-include { SALMON_INDEX }                    from '../modules/nf-core/salmon/index/main'
-include { GFFREAD }                         from '../modules/nf-core/gffread/main'
-
-/*
-========================================================================================
-    RUN MAIN WORKFLOW
-========================================================================================
-*/
-
-workflow BUILD_REFERENCES {
-
-    def fake_meta = [:]
-    fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
-    ENSEMBL_DOWNLOAD( params.ensembl_version, params.genome, fake_meta )
-    HGNC_DOWNLOAD( )
-    SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.primary_assembly, [[],[]])
-    GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.primary_assembly)
-
-    RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
-    CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
-
-    GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
-
-    GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ENSEMBL_DOWNLOAD.out.primary_assembly.map { meta, fasta -> [ fasta ] })
-    SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
-
-    if (params.starindex || params.all || params.starfusion || params.arriba) {
-        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
-    }
-
-    if (params.arriba || params.all) {
-        ARRIBA_DOWNLOAD()
-    }
-
-    if (params.fusioncatcher || params.all) {
-        FUSIONCATCHER_DOWNLOAD()
-    }
-
-    if (params.starfusion || params.all) {
-        if (params.starfusion_build){
-            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
-        } else {
-            STARFUSION_DOWNLOAD()
-        }
-    }
-
-    if (params.starfusion_build){
-        GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.gtf)
-    } else {
-        GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.gtf)
-    }
-
-    if (params.fusionreport || params.all) {
-        FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
-    }
-
-}
-
-/*
-========================================================================================
-    THE END
-========================================================================================
-*/
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 45fd77eb0..bf7fa1b8e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -12,25 +12,6 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi
 include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation'
 
-ch_chrgtf = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
-ch_rrna_interval = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
-ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
-ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
-ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
-ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
-ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
-ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
-
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     IMPORT LOCAL MODULES/SUBWORKFLOWS
@@ -40,7 +21,7 @@ ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
 //
 // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
 //
-
+include { BUILD_REFERENCES              }   from '../subworkflows/local/build_references'
 include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
@@ -75,212 +56,233 @@ workflow RNAFUSION {
 
     main:
 
-    ch_versions = Channel.empty()
-    ch_multiqc_files = Channel.empty()
-
-    //
-    // Create channel from input file provided through params.input
-    //
-    Channel
-        .fromSamplesheet("input")
-        .map {
-            meta, fastq_1, fastq_2, strandedness ->
-                if (!fastq_2) {
-                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
-                } else {
-                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
-                }
-        }
-        .groupTuple()
-        .map {
-            validateInputSamplesheet(it)
-        }
-        .branch {
-            meta, fastqs ->
-                single  : fastqs.size() == 1
-                    return [ meta, fastqs.flatten() ]
-                multiple: fastqs.size() > 1
-                    return [ meta, fastqs.flatten() ]
-        }
-        .set { ch_fastq }
-
-    //
-    // MODULE: Concatenate FastQ files from same sample if required
-    //
-    CAT_FASTQ (
-        ch_fastq.multiple
-    )
-    .reads
-    .mix(ch_fastq.single)
-    .set { ch_cat_fastq }
-    ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
-
 
-    //
-    // MODULE: Run FastQC
-    //
-    FASTQC (
-        ch_cat_fastq
-    )
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    ch_versions = ch_versions.mix(FASTQC.out.versions)
+    // Reference channels
+    // ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
+    // ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
+    // ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
+    // ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
+    // ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
+    // ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
+    // ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
+    // ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
 
-    TRIM_WORKFLOW (
-        ch_cat_fastq
-    )
-    ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
-    ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
-    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
 
-    SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+    ch_versions = Channel.empty()
+    ch_multiqc_files = Channel.empty()
 
 
     //
-    // SUBWORKFLOW:  Run STAR alignment and Arriba
+    // Create references if necessary
     //
-    ARRIBA_WORKFLOW (
-        ch_reads_all,
-        ch_gtf,
-        ch_fasta,
-        ch_starindex_ensembl_ref,
-        ch_arriba_ref_blacklist,
-        ch_arriba_ref_known_fusions,
-        ch_arriba_ref_protein_domains
-    )
-    ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
-
-
-//Run STAR fusion
-    STARFUSION_WORKFLOW (
-        ch_reads_all,
-        ch_chrgtf,
-        ch_starindex_ref,
-        ch_fasta
-    )
-    ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
-
-
-//Run fusioncatcher
-    FUSIONCATCHER_WORKFLOW (
-        ch_reads_fusioncatcher
-    )
-    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
-
-
-//Run stringtie
-    STRINGTIE_WORKFLOW (
-        STARFUSION_WORKFLOW.out.ch_bam_sorted,
-        ch_chrgtf
-    )
-    ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
-
-
-    //Run fusion-report
-    FUSIONREPORT_WORKFLOW (
-        ch_reads_all,
-        ch_fusionreport_ref,
-        ARRIBA_WORKFLOW.out.fusions,
-        STARFUSION_WORKFLOW.out.fusions,
-        FUSIONCATCHER_WORKFLOW.out.fusions
-    )
-    ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
-
-
-    //Run fusionInpector
-    FUSIONINSPECTOR_WORKFLOW (
-        ch_reads_all,
-        FUSIONREPORT_WORKFLOW.out.fusion_list,
-        FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
-        FUSIONREPORT_WORKFLOW.out.report,
-        FUSIONREPORT_WORKFLOW.out.csv,
-        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-        ch_chrgtf,
-        ch_arriba_ref_protein_domains,
-        ch_arriba_ref_cytobands,
-        ch_hgnc_ref,
-        ch_hgnc_date
-    )
-    ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
-
-
-    //QC
-    QC_WORKFLOW (
-        ch_reads_all,
-        STARFUSION_WORKFLOW.out.ch_bam_sorted,
-        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-        ch_chrgtf,
-        ch_refflat,
-        ch_fasta,
-        ch_fai,
-        ch_rrna_interval
-    )
-    ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
 
-    //
-    // Collate and save software versions
-    //
-    softwareVersionsToYAML(ch_versions)
-        .collectFile(
-            storeDir: "${params.outdir}/pipeline_info",
-            name: 'nf_core_pipeline_software_mqc_versions.yml',
-            sort: true,
-            newLine: true
-        ).set { ch_collated_versions }
-
-
-    //
-    // MODULE: MultiQC
-    //
-    ch_multiqc_config        = Channel.fromPath(
-        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-    ch_multiqc_custom_config = params.multiqc_config ?
-        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
-        Channel.empty()
-    ch_multiqc_logo          = params.multiqc_logo ?
-        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
-        Channel.empty()
-
-    summary_params      = paramsSummaryMap(
-        workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
-
-    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
-        file(params.multiqc_methods_description, checkIfExists: true) :
-        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = Channel.value(
-        methodsDescriptionText(ch_multiqc_custom_methods_description))
-
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_methods_description.collectFile(
-            name: 'methods_description_mqc.yaml',
-            sort: true
-        )
-    )
-    ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
-
-    MULTIQC (
-        ch_multiqc_files.collect(),
-        ch_multiqc_config.toList(),
-        ch_multiqc_custom_config.toList(),
-        ch_multiqc_logo.toList()
-    )
-
-    emit:
-    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
-    versions       = ch_versions                 // channel: [ path(versions.yml) ]
+    BUILD_REFERENCES()
+
+
+//     //
+//     // Create channel from input file provided through params.input
+//     //
+//     Channel
+//         .fromSamplesheet("input")
+//         .map {
+//             meta, fastq_1, fastq_2, strandedness ->
+//                 if (!fastq_2) {
+//                     return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
+//                 } else {
+//                     return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
+//                 }
+//         }
+//         .groupTuple()
+//         .map {
+//             validateInputSamplesheet(it)
+//         }
+//         .branch {
+//             meta, fastqs ->
+//                 single  : fastqs.size() == 1
+//                     return [ meta, fastqs.flatten() ]
+//                 multiple: fastqs.size() > 1
+//                     return [ meta, fastqs.flatten() ]
+//         }
+//         .set { ch_fastq }
+
+//     //
+//     // MODULE: Concatenate FastQ files from same sample if required
+//     //
+//     CAT_FASTQ (
+//         ch_fastq.multiple
+//     )
+//     .reads
+//     .mix(ch_fastq.single)
+//     .set { ch_cat_fastq }
+//     ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
+
+
+//     //
+//     // MODULE: Run FastQC
+//     //
+//     FASTQC (
+//         ch_cat_fastq
+//     )
+//     ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+//     ch_versions = ch_versions.mix(FASTQC.out.versions)
+
+//     TRIM_WORKFLOW (
+//         ch_cat_fastq
+//     )
+//     ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
+//     ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
+//     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+
+
+//     SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+
+
+//     //
+//     // SUBWORKFLOW:  Run STAR alignment and Arriba
+//     //
+//     ARRIBA_WORKFLOW (
+//         ch_reads_all,
+//         ch_gtf,
+//         ch_fasta,
+//         ch_starindex_ensembl_ref,
+//         ch_arriba_ref_blacklist,
+//         ch_arriba_ref_known_fusions,
+//         ch_arriba_ref_protein_domains
+//     )
+//     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
+
+
+// //Run STAR fusion
+//     STARFUSION_WORKFLOW (
+//         ch_reads_all,
+//         ch_chrgtf,
+//         ch_starindex_ref,
+//         ch_fasta
+//     )
+//     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+
+
+// //Run fusioncatcher
+//     FUSIONCATCHER_WORKFLOW (
+//         ch_reads_fusioncatcher
+//     )
+//     ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+
+
+// //Run stringtie
+//     STRINGTIE_WORKFLOW (
+//         STARFUSION_WORKFLOW.out.ch_bam_sorted,
+//         ch_chrgtf
+//     )
+//     ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
+
+
+//     //Run fusion-report
+//     FUSIONREPORT_WORKFLOW (
+//         ch_reads_all,
+//         ch_fusionreport_ref,
+//         ARRIBA_WORKFLOW.out.fusions,
+//         STARFUSION_WORKFLOW.out.fusions,
+//         FUSIONCATCHER_WORKFLOW.out.fusions
+//     )
+//     ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
+
+
+//     //Run fusionInpector
+//     FUSIONINSPECTOR_WORKFLOW (
+//         ch_reads_all,
+//         FUSIONREPORT_WORKFLOW.out.fusion_list,
+//         FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
+//         FUSIONREPORT_WORKFLOW.out.report,
+//         FUSIONREPORT_WORKFLOW.out.csv,
+//         STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+//         ch_chrgtf,
+//         ch_arriba_ref_protein_domains,
+//         ch_arriba_ref_cytobands,
+//         ch_hgnc_ref,
+//         ch_hgnc_date
+//     )
+//     ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
+
+
+//     //QC
+//     QC_WORKFLOW (
+//         ch_reads_all,
+//         STARFUSION_WORKFLOW.out.ch_bam_sorted,
+//         STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+//         ch_chrgtf,
+//         ch_refflat,
+//         ch_fasta,
+//         ch_fai,
+//         ch_rrna_interval
+//     )
+//     ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
+
+//     //
+//     // Collate and save software versions
+//     //
+//     softwareVersionsToYAML(ch_versions)
+//         .collectFile(
+//             storeDir: "${params.outdir}/pipeline_info",
+//             name: 'nf_core_pipeline_software_mqc_versions.yml',
+//             sort: true,
+//             newLine: true
+//         ).set { ch_collated_versions }
+
+
+//     //
+//     // MODULE: MultiQC
+//     //
+//     ch_multiqc_config        = Channel.fromPath(
+//         "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+//     ch_multiqc_custom_config = params.multiqc_config ?
+//         Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+//         Channel.empty()
+//     ch_multiqc_logo          = params.multiqc_logo ?
+//         Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+//         Channel.empty()
+
+//     summary_params      = paramsSummaryMap(
+//         workflow, parameters_schema: "nextflow_schema.json")
+//     ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+
+//     ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
+//         file(params.multiqc_methods_description, checkIfExists: true) :
+//         file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+//     ch_methods_description                = Channel.value(
+//         methodsDescriptionText(ch_multiqc_custom_methods_description))
+
+//     ch_multiqc_files = ch_multiqc_files.mix(
+//         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+//     ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
+//     ch_multiqc_files = ch_multiqc_files.mix(
+//         ch_methods_description.collectFile(
+//             name: 'methods_description_mqc.yaml',
+//             sort: true
+//         )
+//     )
+//     ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
+//     ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
+
+//     MULTIQC (
+//         ch_multiqc_files.collect(),
+//         ch_multiqc_config.toList(),
+//         ch_multiqc_custom_config.toList(),
+//         ch_multiqc_logo.toList()
+//     )
+
+//     emit:
+//     multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+//     versions       = ch_versions                 // channel: [ path(versions.yml) ]
 
 }
 

From bf0c33e179290f060a661301e906cb3d24435951 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 28 May 2024 15:39:38 +0200
Subject: [PATCH 030/887] refactor downloading of references

---
 modules/local/fusioncatcher/build/main.nf     |  42 +++++
 modules/local/fusioncatcher/build/meta.yml    |  25 +++
 modules/local/fusioncatcher/download/main.nf  |  30 ++--
 .../local/uscs/custom_gtftogenepred/main.nf   |   1 +
 nextflow.config                               |  30 ++--
 nextflow_schema.json                          |  48 +++---
 subworkflows/local/build_references.nf        | 144 +++++++++++-------
 workflows/rnafusion.nf                        |   9 +-
 8 files changed, 220 insertions(+), 109 deletions(-)
 create mode 100644 modules/local/fusioncatcher/build/main.nf
 create mode 100644 modules/local/fusioncatcher/build/meta.yml

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
new file mode 100644
index 000000000..afab999c0
--- /dev/null
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -0,0 +1,42 @@
+process FUSIONCATCHER_BUILD {
+    tag "fusioncatcher_build"
+    label 'process_medium'
+
+    conda "bioconda::fusioncatcher=1.33"
+    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
+
+    input:
+    val ensembl_version
+
+    output:
+    path "*"                , emit: reference
+    path "versions.yml"     , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+
+    def args = task.ext.args ?: ''
+    """
+    fusioncatcher-build \\
+        -g homo_sapiens \\
+        -o human_${human_version} \\
+        $args
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    mkdir human_v${ensembl_version}
+    touch human_v${ensembl_version}/ensembl_fully_overlapping_genes.txt
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/fusioncatcher/build/meta.yml b/modules/local/fusioncatcher/build/meta.yml
new file mode 100644
index 000000000..40421a4ee
--- /dev/null
+++ b/modules/local/fusioncatcher/build/meta.yml
@@ -0,0 +1,25 @@
+name: fusioncatcher_download
+description: Build genome for fusioncatcher
+keywords:
+  - sort
+tools:
+  - fusioncatcher:
+      description: Build genome for fusioncatcher
+      homepage: https://github.com/ndaniel/fusioncatcher/
+      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
+      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
+      doi: "10.1101/011650"
+      licence: ["GPL v3"]
+
+output:
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - reference:
+      type: directory
+      description: Path to fusioncatcher references
+      pattern: "*"
+
+authors:
+  - "@praveenraj2018, @rannick"
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 156e70b65..7f59d9205 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -5,6 +5,10 @@ process FUSIONCATCHER_DOWNLOAD {
     conda "bioconda::fusioncatcher=1.33"
     container "docker.io/clinicalgenomics/fusioncatcher:1.33"
 
+
+    input:
+    val ensembl_version
+
     output:
     path "*"                , emit: reference
     path "versions.yml"     , emit: versions
@@ -16,22 +20,14 @@ process FUSIONCATCHER_DOWNLOAD {
 
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
-    def human_version = "v102"
-    def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
+    def url =
     """
-    if wget --spider "$url" 2>/dev/null; then
-        wget $args $url
-        wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ab
-        wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ac
-        wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.ad
-        cat human_${human_version}.tar.gz.* | tar xz
-        rm human_${human_version}.tar*
-    else
-        fusioncatcher-build \\
-            -g homo_sapiens \\
-            -o human_${human_version} \\
-            $args2
-    fi
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.aa
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ab
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ac
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ad
+    cat human_${ensembl_version}.tar.gz.* | tar xz
+    rm human_${ensembl_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -40,9 +36,9 @@ process FUSIONCATCHER_DOWNLOAD {
     """
 
     stub:
-    def human_version = "v102"
     """
-    mkdir human_${human_version}
+    mkdir human_v${ensembl_version}
+    touch human_v${ensembl_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
diff --git a/modules/local/uscs/custom_gtftogenepred/main.nf b/modules/local/uscs/custom_gtftogenepred/main.nf
index 78fcbd296..53a74e3d3 100644
--- a/modules/local/uscs/custom_gtftogenepred/main.nf
+++ b/modules/local/uscs/custom_gtftogenepred/main.nf
@@ -1,4 +1,5 @@
 process GTF_TO_REFFLAT {
+    tag "$meta.id"
     label 'process_low'
 
     conda "bioconda::ucsc-gtftogenepred=377"
diff --git a/nextflow.config b/nextflow.config
index 265c3c0b3..fe2dd83da 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -27,19 +27,11 @@ params {
     // Genome
     genome                     = 'GRCh38'
     genomes_base               = "${params.outdir}/references"
-    ensembl_version            = 102
+    ensembl_version            = 112
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
 
-    // Genomes options
-    fasta          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa"
-    fai            = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa.fai"
-    gtf            = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
-    chrgtf         = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
-    refflat        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat"
-    rrna_intervals = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list"
-
     // Filtering
     tools_cutoff               = 1
 
@@ -72,14 +64,21 @@ params {
     skip_qc                    = false
     skip_vis                   = false
 
+    // Download references option
+    download_refs              = true
+
     // Path to references
+    fasta                         = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa"
+    fai                           = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa.fai"
+    gtf                           = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
+    chrgtf                        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
+    refflat                       = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf.refflat"
+    rrna_intervals                = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list"
     ensembl_ref                   = "${params.genomes_base}/ensembl"
-    arriba_ref                    = "${params.genomes_base}/arriba"
     arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz"
-    arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv"
     arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz"
     arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3"
-    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v102"
+    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.ensembl_version}"
     hgnc_ref                      = "${params.genomes_base}/hgnc/hgnc_complete_set.txt"
     hgnc_date                     = "${params.genomes_base}/hgnc/HGNC-DB-timestamp.txt"
     salmon_index                  = "${params.genomes_base}/salmon/salmon"
@@ -88,6 +87,13 @@ params {
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
 
 
+    // Internal file presence checks
+    salmon_index_stub_check       = "${params.genomes_base}/salmon/salmon/complete_ref_lens.bin"
+    starindex_ref_stub_check      = "${params.genomes_base}/star/star/Genome"
+    fusionreport_ref_stub_check   = "${params.genomes_base}/fusion_report_db/mitelman.db"
+    fusioncatcher_ref_stub_check  = "${params.genomes_base}/fusioncatcher/human_v${params.ensembl_version}/ensembl_fully_overlapping_genes.txt"
+    starfusion_ref_stub_check     = "${params.genomes_base}/starfusion/Pfam-A.hmm"
+
     // Path to fusion outputs
     arriba_fusions                = null
     starfusion_fusions            = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 69270631f..0889caa20 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -105,21 +105,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Build or run arriba references/analyses"
                 },
-                "arriba_ref": {
-                    "type": "string",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to arriba references"
-                },
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
                 },
-                "arriba_ref_cytobands": {
-                    "type": "string",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to arriba reference cytobands"
-                },
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -135,6 +125,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba output"
                 },
+                "download_refs": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Download references instead of building them (for fusioncatcher and starfusion)"
+                },
                 "ensembl_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -160,6 +155,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
+                "fusioncatcher_ref_stub_check": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to file in fusioncatcher references"
+                },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
@@ -185,6 +185,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusionreport references"
                 },
+                "fusionreport_ref_stub_check": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to file in fusionreport references"
+                },
                 "hgnc_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -205,6 +210,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to salmon index"
                 },
+                "salmon_index_stub_check": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to file in salmon index"
+                },
                 "starfusion": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -220,6 +230,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starfusion references"
                 },
+                "starfusion_ref_stub_check": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to file in starfusion references"
+                },
                 "starindex": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -230,6 +245,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starindex references"
                 },
+                "starindex_ref_stub_check": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to file in starindex references"
+                },
                 "stringtie": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -326,14 +346,6 @@
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
                 },
-                "transcript": {
-                    "type": "string",
-                    "format": "file-path",
-                    "mimetype": "text/plain",
-                    "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
-                    "description": "Path to GTF genome file.",
-                    "fa_icon": "far fa-file-code"
-                },
                 "refflat": {
                     "type": "string",
                     "format": "file-path",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index bb2acc4d3..0101c4d33 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -7,6 +7,7 @@
 include { ARRIBA_DOWNLOAD }                 from '../../modules/local/arriba/download/main'
 include { ENSEMBL_DOWNLOAD }                from '../../modules/local/ensembl/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../../modules/local/fusioncatcher/download/main'
+include { FUSIONCATCHER_BUILD }             from '../../modules/local/fusioncatcher/build/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../../modules/local/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../../modules/local/starfusion/build/main'
@@ -37,82 +38,117 @@ workflow BUILD_REFERENCES {
 
 
     main:
-
     ch_versions = Channel.empty()
 
-    def fake_meta = [:]
-    fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
 
     if (!file(params.fasta).exists() || file(params.fasta).isEmpty() ||
-                    !file(params.chrgtf).exists() || file(params.chrgtf).isEmpty() ||
-                    !file(params.gtf).exists() || file(params.gtf).isEmpty()){
+            !file(params.chrgtf).exists() || file(params.chrgtf).isEmpty() ||
+            !file(params.gtf).exists() || file(params.gtf).isEmpty()){
+        fake_meta = [:]
+        fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
         ENSEMBL_DOWNLOAD(params.ensembl_version, params.genome, fake_meta)}
-    ch_fasta = {(!file(params.fasta).exists() || file(params.fasta).isEmpty()) ? ENSEMBL_DOWNLOAD.out.primary_assembly : Channel.fromPath(params.fasta).map { that -> [[id:that.Name], that] }.collect()}
+    ch_fasta = Channel.fromPath(params.fasta).map { that -> [[id:that.Name], that] }
+    ch_chrgtf = Channel.fromPath(params.chrgtf).map { that -> [[id:that.Name], that] }
+    ch_gtf = Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }
 
     if (!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
-                    !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()){
+                !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()){
         HGNC_DOWNLOAD( )}
+    ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { that -> [[id:that.Name], that] }
+    ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
+
 
-    if (!file(params.fai).exists() || file(params.fai).isEmpty(){
-        SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.primary_assembly, [[],[]])}
+    if (!file(params.fai).exists() || file(params.fai).isEmpty()){
+        SAMTOOLS_FAIDX(ch_fasta, [[],[]])}
+    ch_fai = Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
 
-    if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty(){
-        GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.primary_assembly)
-        RRNA_TRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf)
+
+    if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty()){
+        GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
+        RRNA_TRANSCRIPTS(ch_gtf)
         CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
         GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
         }
+    ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
+
 
-    if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty(){ // add condition for qc, check that dirs can also be checked with isEmpty()
-        GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ENSEMBL_DOWNLOAD.out.primary_assembly.map { meta, fasta -> [ fasta ] })
-        SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+    if (!file(params.refflat).exists() || file(params.refflat).isEmpty()){
+        GTF_TO_REFFLAT(ch_gtf)}
+    ch_refflat = Channel.fromPath(params.refflat)
+
+
+    if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty() ||
+        !file(params.salmon_index_stub_check).exists() || file(params.salmon_index_stub_check).isEmpty()){ // add condition for qc
+        GFFREAD(ch_gtf, ch_fasta.map{ meta, fasta -> [ fasta ] })
+        SALMON_INDEX(ch_fasta.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
         }
+    ch_salmon_index = Channel.fromPath(params.salmon_index)
 
 
     if ((params.starindex || params.all || params.starfusion || params.arriba) &&
-            (!params.starindex_ref.exits() || params.starindex_ref.isEmpty())
-            ) {
-        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
+            (!file(params.starindex_ref).exists() || file(params.starindex_ref).isEmpty() ||
+            !file(params.starindex_ref_stub_check).exists() || file(params.starindex_ref_stub_check).isEmpty() )) {
+        STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
+    }
+    ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
+
+
+    if ((params.arriba || params.all) &&
+            (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
+            !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
+            !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
+        ARRIBA_DOWNLOAD()}
+    ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
+    ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
+    ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
+
+
+    if ((params.fusioncatcher || params.all) &&
+            (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
+            !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
+        if (params.download_refs) {
+            FUSIONCATCHER_DOWNLOAD(params.ensembl_version)}
+        else {
+            FUSIONCATCHER_BUILD(params.ensembl_version)}
     }
-    ch_starindex_ref = ...
-
-    // if (params.arriba || params.all) {
-    //     ARRIBA_DOWNLOAD()
-    // }
-
-    // if (params.fusioncatcher || params.all) {
-    //     FUSIONCATCHER_DOWNLOAD()
-    // }
-
-    // if (params.starfusion || params.all) {
-    //     if (params.starfusion_build){
-    //         STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
-    //     } else {
-    //         STARFUSION_DOWNLOAD()
-    //     }
-    // }
-
-    // if (params.starfusion_build){
-    //     GTF_TO_REFFLAT(ENSEMBL_DOWNLOAD.out.gtf)
-    // } else {
-    //     GTF_TO_REFFLAT(STARFUSION_DOWNLOAD.out.gtf)
-    // }
-
-    // if (params.fusionreport || params.all) {
-    //     FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
-    // }
+    ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
 
-    emit:
-    ch_fasta
-    ch_chrgtf = {(!file(params.chrgtf).exists() || file(params.chrgtf).isEmpty()) ? ENSEMBL_DOWNLOAD.out.chrgtf : Channel.fromPath(params.chrgtf).map { that -> [[id:that.Name], that] }.collect()}
-    ch_gtf = {(!file(params.gtf).exists() || file(params.gtf).isEmpty()) ? ENSEMBL_DOWNLOAD.out.gtf : Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }.collect()}
-    ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
-    ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
-    ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
-    ch_rrna_interval = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
-    ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
+
+    if ((params.starfusion || params.all) &&
+            (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
+            !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
+        if (params.download_refs) {
+            STARFUSION_DOWNLOAD( ch_fasta, ch_gtf )}
+        else {
+            STARFUSION_BUILD( ch_fasta, ch_gtf )}
+    }
+    ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }
 
 
+    if ((params.fusionreport || params.all) &&
+            (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
+            !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
+        if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
+        FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )}
+    ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
+
+    emit:
+    ch_fasta
+    ch_chrgtf
+    ch_gtf
+    ch_hgnc_ref
+    ch_hgnc_date
+    ch_fai
+    ch_rrna_interval
+    ch_refflat
+    ch_salmon_index
+    ch_starindex_ref
+    ch_arriba_ref_blacklist
+    ch_arriba_ref_known_fusions
+    ch_arriba_ref_protein_domains
+    ch_fusioncatcher_ref
+    ch_starfusion_ref
+    ch_fusionreport_ref
 }
 
 /*
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bf7fa1b8e..64bd3f99c 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -58,14 +58,7 @@ workflow RNAFUSION {
 
 
     // Reference channels
-    // ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
-    // ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
-    // ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
-    // ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
-    // ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
-    // ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
-    // ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
-    // ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
+
 
 
 

From a19abbde7f1268bf49b5e57a7485040fc592468d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 28 May 2024 21:14:21 +0200
Subject: [PATCH 031/887] fix params

---
 modules/local/fusioncatcher/build/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index afab999c0..c5eeeb451 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -21,7 +21,7 @@ process FUSIONCATCHER_BUILD {
     """
     fusioncatcher-build \\
         -g homo_sapiens \\
-        -o human_${human_version} \\
+        -o human_v${ensembl_version} \\
         $args
 
     cat <<-END_VERSIONS > versions.yml

From 93fbfb8bc795c3fe405d9e98c6199469e213e203 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 28 May 2024 21:25:30 +0200
Subject: [PATCH 032/887] fix

---
 modules/local/fusioncatcher/build/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index c5eeeb451..513535567 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -19,7 +19,7 @@ process FUSIONCATCHER_BUILD {
 
     def args = task.ext.args ?: ''
     """
-    fusioncatcher-build \\
+    fusioncatcher-build.py \\
         -g homo_sapiens \\
         -o human_v${ensembl_version} \\
         $args

From 49c133719ad62d6610bb9d0fd6e1a9e4b81aef73 Mon Sep 17 00:00:00 2001
From: asp8200 <anders@mynucleus.com>
Date: Tue, 11 Jun 2024 12:19:34 +0000
Subject: [PATCH 033/887] update docs

---
 README.md     |  6 ++----
 docs/usage.md | 12 ++++++++++++
 2 files changed, 14 insertions(+), 4 deletions(-)

diff --git a/README.md b/README.md
index f34ee57ec..4324f2109 100644
--- a/README.md
+++ b/README.md
@@ -76,8 +76,7 @@ First, build the references:
 
 ```bash
 nextflow run nf-core/rnafusion \
-   -profile <docker/singularity/.../institute> \
-   -profile test \
+   -profile test,<docker/singularity/.../institute> \
    --outdir <OUTDIR>\
    --build_references \
    -stub
@@ -87,8 +86,7 @@ Then perform the analysis:
 
 ```bash
 nextflow run nf-core/rnafusion \
-   -profile <docker/singularity/.../institute> \
-   -profile test \
+   -profile test,<docker/singularity/.../institute> \
    --outdir <OUTDIR>\
    -stub
 ```
diff --git a/docs/usage.md b/docs/usage.md
index 6ed114706..80ff457c5 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -31,6 +31,7 @@ The rnafusion pipeline needs references for the fusion detection tools, so downl
 
 ```bash
 nextflow run nf-core/rnafusion \
+  -profile <docker/singularity/.../institute> \
   --build_references --all \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -41,6 +42,7 @@ References for each tools can also be downloaded separately with:
 
 ```bash
 nextflow run nf-core/rnafusion \
+  -profile <docker/singularity/.../institute> \
   --build_references --<tool1> --<tool2> ... \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -59,6 +61,7 @@ Use credentials from QIAGEN and add `--qiagen`
 
 ```bash
 nextflow run nf-core/rnafusion \
+  -profile <docker/singularity/.../institute> \
   --build_references --<tool1> --<tool2> ... \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -75,6 +78,7 @@ If process `FUSIONREPORT_DOWNLOAD` times out, it could be due to network restric
 
 ```bash
 nextflow run nf-core/rnafusion  \
+  -profile <docker/singularity/.../institute> \
   --build_references \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --fusionreport \
@@ -134,6 +138,7 @@ The pipeline can either be run using all fusion detection tools or specifying in
 
 ```bash
 nextflow run nf-core/rnafusion \
+  -profile <docker/singularity/.../institute> \
   --all \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -144,6 +149,7 @@ To run only a specific detection tool use: `--tool`:
 
 ```bash
 nextflow run nf-core/rnafusion \
+  -profile <docker/singularity/.../institute> \
   --<tool1> --<tool2> ... \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -200,6 +206,7 @@ When the flag `--fastp_trim` is used, `fastp` is used to provide all tools with
 
 ```bash
 nextflow run nf-core/rnafusion \
+-profile <docker/singularity/.../institute> \
 --<tool1> --<tool2> ... \
 --input <SAMPLE_SHEET.CSV> \
 --genomes_base <PATH/TO/REFERENCES> \
@@ -213,6 +220,7 @@ nextflow run nf-core/rnafusion \
 
 ```bash
 nextflow run nf-core/rnafusion \
+  -profile <docker/singularity/.../institute> \
   --<tool1> --<tool2> ... \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -226,6 +234,7 @@ nextflow run nf-core/rnafusion \
 
 ```bash
 nextflow run nf-core/rnafusion \
+  -profile <docker/singularity/.../institute> \
   --<tool1> --<tool2> ... \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -246,6 +255,7 @@ FusionInspector can be run as a standalone with:
 
 ```bash
 nextflow run nf-core/rnafusion \
+-profile <docker/singularity/.../institute> \
 --fusioninspector_only \
 --fusioninspector_fusions <PATH_TO_CUSTOM_FUSION_FILE> \
 --input <SAMPLE_SHEET.CSV> \
@@ -263,6 +273,7 @@ GENE3--GENE4
 
 ```bash
 nextflow run nf-core/rnafusion \
+-profile <docker/singularity/.../institute> \
 --skip_qc \
 --all OR <--tool>
 --input <SAMPLE_SHEET.CSV> \
@@ -276,6 +287,7 @@ This will skip all QC-related processes (picard metrics collection)
 
 ```bash
 nextflow run nf-core/rnafusion \
+-profile <docker/singularity/.../institute> \
 --skip_vis \
 --all OR <--tool>
 --input <SAMPLE_SHEET.CSV> \

From 5be23dbc44ca63c18355420751b0c45b2b9fce23 Mon Sep 17 00:00:00 2001
From: asp8200 <anders@mynucleus.com>
Date: Tue, 11 Jun 2024 12:22:23 +0000
Subject: [PATCH 034/887] update changelog

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5effdca59..be2b037dd 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,6 +15,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Fixed
 
+- Fixed some Nextflow run-commands in the docs [#491](https://github.com/nf-core/rnafusion/pull/491)
+
 ### Removed
 
 ## v3.0.2 - [2024-04-10]

From b7dd1375cd74fa4c9b37edc415c665976b708930 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 13 Jun 2024 11:41:44 +0200
Subject: [PATCH 035/887] update refs generation

---
 conf/modules.config                          |  12 ++-
 modules/local/fusioncatcher/build/main.nf    |   4 +-
 modules/local/fusioncatcher/download/main.nf |   2 +-
 nextflow.config                              |   1 +
 nextflow_schema.json                         |   4 +
 subworkflows/local/build_references.nf       | 101 +++++++++++-------
 workflows/rnafusion.nf                       | 106 +++++++++----------
 7 files changed, 131 insertions(+), 99 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 44dec3397..e78f73dbb 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -45,6 +45,10 @@ process {
         ]
     }
 
+    withName: 'CAT_FASTQ' {
+        ext.when   = { !params.build_references }
+    }
+
     withName: 'ENSEMBL_DOWNLOAD' {
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },
@@ -59,7 +63,7 @@ process {
 
     withName: 'FASTQC' {
         ext.args = '--quiet'
-        ext.when = { !params.skip_qc }
+        ext.when = { !params.skip_qc and not !params.build_references }
         publishDir = [
             path: { "${params.outdir}/fastqc" },
             mode: params.publish_dir_mode,
@@ -69,6 +73,7 @@ process {
 
     withName: 'FASTQC_FOR_FASTP' {
         ext.args = '--quiet'
+        ext.when = ( !params.skip_qc)
         ext.prefix = { "${meta.id}_trimmed" }
         publishDir = [
             path: { "${params.outdir}/fastqc_for_fastp" },
@@ -362,6 +367,11 @@ process {
         ]
     }
 
+    withName: 'TRIM_WORKFLOW:*' {
+        ext.when = { !params.build_references }
+
+    }
+
     withName: 'VCF_COLLECT' {
         ext.when = {!params.fusioninspector_only}
     }
diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 513535567..15f7afedc 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -9,8 +9,8 @@ process FUSIONCATCHER_BUILD {
     val ensembl_version
 
     output:
-    path "*"                , emit: reference
-    path "versions.yml"     , emit: versions
+    path "human_v${ensembl_version}"  , emit: reference
+    path "versions.yml"               , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 7f59d9205..ff146c95c 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -10,7 +10,7 @@ process FUSIONCATCHER_DOWNLOAD {
     val ensembl_version
 
     output:
-    path "*"                , emit: reference
+    path "human_v${ensembl_version}"                , emit: reference
     path "versions.yml"     , emit: versions
 
     when:
diff --git a/nextflow.config b/nextflow.config
index fe2dd83da..b7d78f7d9 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -62,6 +62,7 @@ params {
 
     // Skip steps
     skip_qc                    = false
+    skip_vcf                   = false
     skip_vis                   = false
 
     // Download references option
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 0889caa20..604e7b00a 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -15,6 +15,10 @@
                     "type": "boolean",
                     "description": "Skip QC steps"
                 },
+                "skip_vcf": {
+                    "type": "boolean",
+                    "description": "Skip vcf creation step"
+                }
                 "skip_vis": {
                     "type": "boolean",
                     "description": "Skip visualisation steps"
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 0101c4d33..32602cc1e 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -46,21 +46,30 @@ workflow BUILD_REFERENCES {
             !file(params.gtf).exists() || file(params.gtf).isEmpty()){
         fake_meta = [:]
         fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
-        ENSEMBL_DOWNLOAD(params.ensembl_version, params.genome, fake_meta)}
-    ch_fasta = Channel.fromPath(params.fasta).map { that -> [[id:that.Name], that] }
-    ch_chrgtf = Channel.fromPath(params.chrgtf).map { that -> [[id:that.Name], that] }
-    ch_gtf = Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }
+        ENSEMBL_DOWNLOAD(params.ensembl_version, params.genome, fake_meta)
+        ch_fasta = ENSEMBL_DOWNLOAD.out.primary_assembly
+        ch_chrgtf = ENSEMBL_DOWNLOAD.out.chrgtf
+        ch_gtf = ENSEMBL_DOWNLOAD.out.gtf
+    } else {
+        ch_fasta = Channel.fromPath(params.fasta).map { that -> [[id:that.Name], that] }
+        ch_chrgtf = Channel.fromPath(params.chrgtf).map { that -> [[id:that.Name], that] }
+        ch_gtf = Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }
+    }
+
 
-    if (!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
-                !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()){
-        HGNC_DOWNLOAD( )}
-    ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { that -> [[id:that.Name], that] }
-    ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
+    if ((!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
+                !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()) and not params.skip_vcf){
+        HGNC_DOWNLOAD( )
+        ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref
+        ch_hgnc_date =HGNC_DOWNLOAD.out.hgnc_date
 
+    } else {
+        ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { that -> [[id:that.Name], that] }
+        ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
+    }
 
-    if (!file(params.fai).exists() || file(params.fai).isEmpty()){
-        SAMTOOLS_FAIDX(ch_fasta, [[],[]])}
-    ch_fai = Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
+
+    ch_fai = (!file(params.fai).exists() || file(params.fai).isEmpty()) ? SAMTOOLS_FAIDX(ch_fasta, [[],[]]).fai : Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
 
 
     if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty()){
@@ -68,69 +77,81 @@ workflow BUILD_REFERENCES {
         RRNA_TRANSCRIPTS(ch_gtf)
         CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
         GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
-        }
-    ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
+        ch_rrna_interval = GATK4_BEDTOINTERVALLIST.out.interval_list
+    } else {
+        ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
+    }
 
 
-    if (!file(params.refflat).exists() || file(params.refflat).isEmpty()){
-        GTF_TO_REFFLAT(ch_gtf)}
-    ch_refflat = Channel.fromPath(params.refflat)
+    ch_refflat = (!file(params.refflat).exists() || file(params.refflat).isEmpty()) ? GTF_TO_REFFLAT.refflat : Channel.fromPath(params.refflat)
 
 
     if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty() ||
         !file(params.salmon_index_stub_check).exists() || file(params.salmon_index_stub_check).isEmpty()){ // add condition for qc
         GFFREAD(ch_gtf, ch_fasta.map{ meta, fasta -> [ fasta ] })
         SALMON_INDEX(ch_fasta.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+        ch_salmon_index = SALMON_INDEX.out.index
+    } else {
+        ch_salmon_index = Channel.fromPath(params.salmon_index)
         }
-    ch_salmon_index = Channel.fromPath(params.salmon_index)
 
 
     if ((params.starindex || params.all || params.starfusion || params.arriba) &&
             (!file(params.starindex_ref).exists() || file(params.starindex_ref).isEmpty() ||
             !file(params.starindex_ref_stub_check).exists() || file(params.starindex_ref_stub_check).isEmpty() )) {
         STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
+        ch_starindex_ref = STAR_GENOMEGENERATE.out.index
+    } else {
+        ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
     }
-    ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
 
 
-    if ((params.arriba || params.all) &&
-            (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
-            !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
-            !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
-        ARRIBA_DOWNLOAD()}
-    ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
-    ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
-    ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
+//     if ((params.arriba || params.all) &&
+//             (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
+//             !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
+//             !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
+//         ARRIBA_DOWNLOAD()
+//         ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
+//         ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
+//         ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
+//     } else {
+// // need to update the module to emit blacklist,knownfusions etc
+//     }
 
 
     if ((params.fusioncatcher || params.all) &&
             (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
             !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
         if (params.download_refs) {
-            FUSIONCATCHER_DOWNLOAD(params.ensembl_version)}
+            FUSIONCATCHER_DOWNLOAD(params.ensembl_version)
+            ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference}
         else {
-            FUSIONCATCHER_BUILD(params.ensembl_version)}
-    }
-    ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
+            FUSIONCATCHER_BUILD(params.ensembl_version)
+            ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference}
+        } else {
+            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
+        }
 
 
     if ((params.starfusion || params.all) &&
             (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
             !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
         if (params.download_refs) {
-            STARFUSION_DOWNLOAD( ch_fasta, ch_gtf )}
+            ch_starfusion_ref = STARFUSION_DOWNLOAD( ch_fasta, ch_gtf ).out.reference }
         else {
-            STARFUSION_BUILD( ch_fasta, ch_gtf )}
-    }
-    ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }
+            ch_starfusion_ref = STARFUSION_BUILD( ch_fasta, ch_gtf ).out.reference }
+    } else {
+        ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }}
 
 
     if ((params.fusionreport || params.all) &&
             (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
             !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
         if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
-        FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )}
-    ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
+        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd ).out.reference
+    } else {
+        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
+    }
 
     emit:
     ch_fasta
@@ -143,9 +164,9 @@ workflow BUILD_REFERENCES {
     ch_refflat
     ch_salmon_index
     ch_starindex_ref
-    ch_arriba_ref_blacklist
-    ch_arriba_ref_known_fusions
-    ch_arriba_ref_protein_domains
+    // ch_arriba_ref_blacklist
+    // ch_arriba_ref_known_fusions
+    // ch_arriba_ref_protein_domains
     ch_fusioncatcher_ref
     ch_starfusion_ref
     ch_fusionreport_ref
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 64bd3f99c..eb0e19f49 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -57,11 +57,6 @@ workflow RNAFUSION {
     main:
 
 
-    // Reference channels
-
-
-
-
     ch_versions = Channel.empty()
     ch_multiqc_files = Channel.empty()
 
@@ -73,59 +68,60 @@ workflow RNAFUSION {
     BUILD_REFERENCES()
 
 
-//     //
-//     // Create channel from input file provided through params.input
-//     //
-//     Channel
-//         .fromSamplesheet("input")
-//         .map {
-//             meta, fastq_1, fastq_2, strandedness ->
-//                 if (!fastq_2) {
-//                     return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
-//                 } else {
-//                     return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
-//                 }
-//         }
-//         .groupTuple()
-//         .map {
-//             validateInputSamplesheet(it)
-//         }
-//         .branch {
-//             meta, fastqs ->
-//                 single  : fastqs.size() == 1
-//                     return [ meta, fastqs.flatten() ]
-//                 multiple: fastqs.size() > 1
-//                     return [ meta, fastqs.flatten() ]
-//         }
-//         .set { ch_fastq }
-
-//     //
-//     // MODULE: Concatenate FastQ files from same sample if required
-//     //
-//     CAT_FASTQ (
-//         ch_fastq.multiple
-//     )
-//     .reads
-//     .mix(ch_fastq.single)
-//     .set { ch_cat_fastq }
-//     ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
+    //
+    // Create channel from input file provided through params.input
+    //
+    Channel
+        .fromSamplesheet("input")
+        .map {
+            meta, fastq_1, fastq_2, strandedness ->
+                if (!fastq_2) {
+                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
+                } else {
+                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
+                }
+        }
+        .groupTuple()
+        .map {
+            validateInputSamplesheet(it)
+        }
+        .branch {
+            meta, fastqs ->
+                single  : fastqs.size() == 1
+                    return [ meta, fastqs.flatten() ]
+                multiple: fastqs.size() > 1
+                    return [ meta, fastqs.flatten() ]
+        }
+        .set { ch_fastq }
 
+    //
+    // MODULE: Concatenate FastQ files from same sample if required
+    //
+    CAT_FASTQ (
+        ch_fastq.multiple
+    )
+    .reads
+    .mix(ch_fastq.single)
+    .set { ch_cat_fastq }
+    ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
 
-//     //
-//     // MODULE: Run FastQC
-//     //
-//     FASTQC (
-//         ch_cat_fastq
-//     )
-//     ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-//     ch_versions = ch_versions.mix(FASTQC.out.versions)
 
-//     TRIM_WORKFLOW (
-//         ch_cat_fastq
-//     )
-//     ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
-//     ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
-//     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+    //
+    // MODULE: Run FastQC
+    //
+    FASTQC (
+        ch_cat_fastq
+    )
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+    ch_versions = ch_versions.mix(FASTQC.out.versions)
+
+
+    TRIM_WORKFLOW (
+        ch_cat_fastq
+    )
+    ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
+    ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
+    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
 
 //     SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')

From 89be7c1ab16c8a25dc7dedc8cdc7a18a3c36d75f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 13 Jun 2024 12:15:12 +0200
Subject: [PATCH 036/887] fix small issues

---
 modules/local/fusioncatcher/build/main.nf |  2 +-
 nextflow_schema.json                      |  2 +-
 subworkflows/local/build_references.nf    |  5 ++---
 workflows/rnafusion.nf                    | 10 +++++-----
 4 files changed, 9 insertions(+), 10 deletions(-)

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 15f7afedc..0258a2cca 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER_BUILD {
     label 'process_medium'
 
     conda "bioconda::fusioncatcher=1.33"
-    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
+    container "docker.io/rannickscilifelab/fusioncatcher:1.33a"
 
     input:
     val ensembl_version
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 604e7b00a..95238d65d 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -18,7 +18,7 @@
                 "skip_vcf": {
                     "type": "boolean",
                     "description": "Skip vcf creation step"
-                }
+                },
                 "skip_vis": {
                     "type": "boolean",
                     "description": "Skip visualisation steps"
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 32602cc1e..49a18ce2a 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -58,11 +58,10 @@ workflow BUILD_REFERENCES {
 
 
     if ((!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
-                !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()) and not params.skip_vcf){
+            !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()) && !params.skip_vcf){
         HGNC_DOWNLOAD( )
         ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref
-        ch_hgnc_date =HGNC_DOWNLOAD.out.hgnc_date
-
+        ch_hgnc_date = HGNC_DOWNLOAD.out.hgnc_date
     } else {
         ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { that -> [[id:that.Name], that] }
         ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index eb0e19f49..8067210a8 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -109,11 +109,11 @@ workflow RNAFUSION {
     //
     // MODULE: Run FastQC
     //
-    FASTQC (
-        ch_cat_fastq
-    )
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    ch_versions = ch_versions.mix(FASTQC.out.versions)
+    // FASTQC (
+    //     ch_cat_fastq
+    // )
+    // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+    // ch_versions = ch_versions.mix(FASTQC.out.versions)
 
 
     TRIM_WORKFLOW (

From 0b854c6f75ae63ef683f8a0d0b04897f98920522 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alexander.peltzer@boehringer-ingelheim.com>
Date: Thu, 8 Aug 2024 15:43:35 +0200
Subject: [PATCH 037/887] Add option to wget to ignore proxy certificates

---
 modules/local/arriba/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/arriba/download/main.nf b/modules/local/arriba/download/main.nf
index 860439ade..61b9a91f7 100644
--- a/modules/local/arriba/download/main.nf
+++ b/modules/local/arriba/download/main.nf
@@ -13,7 +13,7 @@ process ARRIBA_DOWNLOAD {
 
     script:
     """
-    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz
+    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz --no-check-certificate
     tar -xzvf arriba_v2.4.0.tar.gz
     rm arriba_v2.4.0.tar.gz
     mv arriba_v2.4.0/database/* .

From 8f8eeaa28a17ea76ab871c80dba5fe641acc7441 Mon Sep 17 00:00:00 2001
From: Alexander Peltzer <alex.peltzer@gmail.com>
Date: Thu, 8 Aug 2024 14:59:49 +0000
Subject: [PATCH 038/887] Add changelog

---
 CHANGELOG.md | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 871c46594..21af3e270 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,12 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## dev
+
+## Fixed
+
+- fix bug when trying to build indices behind a proxy and wget was unable to download arriba indices [[#495](https://github.com/nf-core/rnafusion/issues/495)]
+
 ## v3.0.2 - [2024-04-10]
 
 ### Added

From 0c6fe8283c61f0dc4557970ce576d08fc97ee909 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 Sep 2024 11:21:33 +0200
Subject: [PATCH 039/887] updates

---
 conf/base.config                             |  2 +-
 modules/local/fusioncatcher/build/main.nf    |  2 +-
 modules/local/fusioncatcher/download/main.nf |  1 +
 modules/local/starfusion/download/main.nf    |  1 +
 subworkflows/local/build_references.nf       | 30 ++++++++++----------
 subworkflows/local/trim_workflow.nf          | 18 ++++--------
 workflows/rnafusion.nf                       | 24 +++++++++-------
 7 files changed, 38 insertions(+), 40 deletions(-)

diff --git a/conf/base.config b/conf/base.config
index 5b2e6f360..62d8dbf15 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -31,7 +31,7 @@ process {
     }
     withLabel:process_medium {
         cpus   = { check_max( 6     * task.attempt, 'cpus'    ) }
-        memory = { check_max( 36.GB * task.attempt, 'memory'  ) }
+        memory = { check_max( 16.GB * task.attempt, 'memory'  ) }
         time   = { check_max( 8.h   * task.attempt, 'time'    ) }
     }
     withLabel:process_high {
diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 0258a2cca..15f7afedc 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER_BUILD {
     label 'process_medium'
 
     conda "bioconda::fusioncatcher=1.33"
-    container "docker.io/rannickscilifelab/fusioncatcher:1.33a"
+    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
 
     input:
     val ensembl_version
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index ff146c95c..aec162882 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,6 +22,7 @@ process FUSIONCATCHER_DOWNLOAD {
     def args2 = task.ext.args2 ?: ''
     def url =
     """
+    TODO: move to my sourceforge
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.aa
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ab
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ac
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 9c9b14827..e3a4b1b85 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -9,6 +9,7 @@ process STARFUSION_DOWNLOAD {
     path "ctat_genome_lib_build_dir/ref_annot.gtf", emit: chrgtf
 
 
+    TODO: move to my sourceforge
     script:
     """
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.10/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 49a18ce2a..a1a640509 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -92,7 +92,7 @@ workflow BUILD_REFERENCES {
         ch_salmon_index = SALMON_INDEX.out.index
     } else {
         ch_salmon_index = Channel.fromPath(params.salmon_index)
-        }
+    }
 
 
     if ((params.starindex || params.all || params.starfusion || params.arriba) &&
@@ -105,17 +105,17 @@ workflow BUILD_REFERENCES {
     }
 
 
-//     if ((params.arriba || params.all) &&
-//             (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
-//             !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
-//             !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
-//         ARRIBA_DOWNLOAD()
-//         ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
-//         ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
-//         ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
-//     } else {
-// // need to update the module to emit blacklist,knownfusions etc
-//     }
+    if ((params.arriba || params.all) &&
+            (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
+            !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
+            !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
+        ARRIBA_DOWNLOAD()
+        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
+        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
+        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
+    } else {
+// TODO need to update the module to emit blacklist,knownfusions etc
+    }
 
 
     if ((params.fusioncatcher || params.all) &&
@@ -163,9 +163,9 @@ workflow BUILD_REFERENCES {
     ch_refflat
     ch_salmon_index
     ch_starindex_ref
-    // ch_arriba_ref_blacklist
-    // ch_arriba_ref_known_fusions
-    // ch_arriba_ref_protein_domains
+    ch_arriba_ref_blacklist
+    ch_arriba_ref_known_fusions
+    ch_arriba_ref_protein_domains
     ch_fusioncatcher_ref
     ch_starfusion_ref
     ch_fusionreport_ref
diff --git a/subworkflows/local/trim_workflow.nf b/subworkflows/local/trim_workflow.nf
index ea21134db..f963451bc 100644
--- a/subworkflows/local/trim_workflow.nf
+++ b/subworkflows/local/trim_workflow.nf
@@ -8,9 +8,6 @@ workflow TRIM_WORKFLOW {
 
     main:
         ch_versions = Channel.empty()
-        ch_fastp_html = Channel.empty()
-        ch_fastp_json = Channel.empty()
-        ch_fastqc_trimmed = Channel.empty()
 
         if (params.fastp_trim) {
             FASTP(reads, params.adapter_fasta, false, false)
@@ -19,24 +16,21 @@ workflow TRIM_WORKFLOW {
             FASTQC_FOR_FASTP(FASTP.out.reads)
             ch_versions = ch_versions.mix(FASTQC_FOR_FASTP.out.versions)
 
-            ch_reads_all = FASTP.out.reads
-            ch_reads_fusioncatcher = ch_reads_all
+            ch_reads = FASTP.out.reads
             ch_fastp_html = FASTP.out.html
             ch_fastp_json = FASTP.out.json
             ch_fastqc_trimmed = FASTQC_FOR_FASTP.out.zip
 
         }
         else {
-            ch_reads_all = reads
-            ch_reads_fusioncatcher = reads
+            ch_reads = reads
         }
 
     emit:
-        ch_reads_all
-        ch_reads_fusioncatcher
-        ch_fastp_html
-        ch_fastp_json
-        ch_fastqc_trimmed
+        trimmed_reads = ch_reads
+        fastp_html = ch_fastp_html.ifEmpty([])
+        fastp_json = ch_fastp_json.ifEmpty([])
+        fastqc_trimmed = ch_fastqc_trimmed.ifEmpty([])
         versions = ch_versions
     }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 8067210a8..302f2829f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -107,26 +107,28 @@ workflow RNAFUSION {
 
 
     //
-    // MODULE: Run FastQC
+    // QC from FASTQ files
     //
-    // FASTQC (
-    //     ch_cat_fastq
-    // )
-    // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    // ch_versions = ch_versions.mix(FASTQC.out.versions)
+    FASTQC (
+        ch_cat_fastq
+    )
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+    ch_versions = ch_versions.mix(FASTQC.out.versions)
 
+    SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+    ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.zip.collect{it[1]})
+    ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
 
+    //
+    // Trimming
+    //
     TRIM_WORKFLOW (
         ch_cat_fastq
     )
-    ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
-    ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
+    ch_reads = TRIM_WORKFLOW.out.trimmed_reads
     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
 
-//     SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
-
-
 //     //
 //     // SUBWORKFLOW:  Run STAR alignment and Arriba
 //     //

From 40da42dc561fbbf8ec9a6a6dd2f18d67eaeec118 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 Sep 2024 11:33:15 +0200
Subject: [PATCH 040/887] update modules again

---
 modules.json                                  |   56 +-
 .../agat/convertspgff2tsv/environment.yml     |    2 -
 .../agat/convertspgff2tsv/tests/main.nf.test  |    4 +-
 modules/nf-core/arriba/arriba/environment.yml |    2 -
 .../nf-core/arriba/download/environment.yml   |    2 -
 modules/nf-core/arriba/download/main.nf       |    2 +
 .../arriba/download/tests/main.nf.test        |   35 +
 .../arriba/download/tests/main.nf.test.snap   |   35 +
 .../bedops/convert2bed/environment.yml        |    2 -
 .../bedops/convert2bed/tests/main.nf.test     |    4 +-
 modules/nf-core/cat/cat/environment.yml       |    2 -
 modules/nf-core/cat/cat/main.nf               |    1 -
 modules/nf-core/cat/cat/tests/main.nf.test    |   27 +-
 .../nf-core/cat/cat/tests/main.nf.test.snap   |   74 +-
 modules/nf-core/cat/fastq/environment.yml     |    2 -
 modules/nf-core/cat/fastq/main.nf             |   10 +-
 modules/nf-core/cat/fastq/tests/main.nf.test  |  136 +-
 .../nf-core/cat/fastq/tests/main.nf.test.snap |  207 ++
 modules/nf-core/fastp/environment.yml         |    2 -
 modules/nf-core/fastp/main.nf                 |   21 +-
 modules/nf-core/fastp/meta.yml                |    6 +-
 modules/nf-core/fastp/tests/main.nf.test      |  823 +++---
 modules/nf-core/fastp/tests/main.nf.test.snap | 1361 ++++++++--
 modules/nf-core/fastqc/environment.yml        |    2 -
 modules/nf-core/fastqc/main.nf                |    5 +-
 modules/nf-core/fastqc/tests/main.nf.test     |  225 +-
 .../nf-core/fastqc/tests/main.nf.test.snap    |  370 ++-
 .../gatk4/bedtointervallist/environment.yml   |    2 -
 .../createsequencedictionary/environment.yml  |    2 -
 .../gatk4/markduplicates/environment.yml      |    2 -
 modules/nf-core/gffread/environment.yml       |    2 -
 modules/nf-core/multiqc/environment.yml       |    4 +-
 modules/nf-core/multiqc/main.nf               |   14 +-
 modules/nf-core/multiqc/meta.yml              |   13 +
 modules/nf-core/multiqc/tests/main.nf.test    |    8 +
 .../nf-core/multiqc/tests/main.nf.test.snap   |   20 +-
 modules/nf-core/multiqc/tests/nextflow.config |    5 +
 .../collectinsertsizemetrics/environment.yml  |    4 +-
 .../picard/collectinsertsizemetrics/main.nf   |    8 +-
 .../tests/main.nf.test                        |   62 +
 .../tests/main.nf.test.snap                   |   74 +
 .../collectrnaseqmetrics/environment.yml      |    4 +-
 .../picard/collectrnaseqmetrics/main.nf       |    4 +-
 .../picard/collectwgsmetrics/environment.yml  |    4 +-
 .../nf-core/picard/collectwgsmetrics/main.nf  |    4 +-
 .../collectwgsmetrics/tests/main.nf.test      |   83 +
 .../collectwgsmetrics/tests/main.nf.test.snap |   28 +
 .../nf-core/rrnatranscripts/environment.yml   |    2 -
 .../templates/get_rrna_transcripts.py         |    8 +-
 .../rrnatranscripts/tests/main.nf.test        |    4 +-
 modules/nf-core/salmon/index/environment.yml  |    2 -
 modules/nf-core/salmon/quant/environment.yml  |    2 -
 modules/nf-core/salmon/quant/main.nf          |   11 +-
 .../nf-core/salmon/quant/tests/main.nf.test   |   40 +-
 .../salmon/quant/tests/main.nf.test.snap      |  123 +-
 .../nf-core/samtools/faidx/environment.yml    |    7 +-
 modules/nf-core/samtools/faidx/main.nf        |    4 +-
 .../samtools/faidx/tests/main.nf.test.snap    |   30 +-
 .../nf-core/samtools/index/environment.yml    |    6 +-
 modules/nf-core/samtools/index/main.nf        |   11 +-
 .../nf-core/samtools/index/tests/main.nf.test |   87 +-
 .../samtools/index/tests/main.nf.test.snap    |  264 +-
 modules/nf-core/samtools/sort/environment.yml |    6 +-
 modules/nf-core/samtools/sort/main.nf         |   18 +-
 .../nf-core/samtools/sort/tests/main.nf.test  |   52 +-
 .../samtools/sort/tests/main.nf.test.snap     |  144 +-
 .../samtools/sort/tests/nextflow_cram.config  |    8 +
 modules/nf-core/samtools/view/environment.yml |    6 +-
 modules/nf-core/samtools/view/main.nf         |   44 +-
 modules/nf-core/samtools/view/meta.yml        |    9 +
 .../nf-core/samtools/view/tests/main.nf.test  |    2 +
 .../samtools/view/tests/main.nf.test.snap     |   58 +-
 modules/nf-core/star/align/environment.yml    |    2 -
 modules/nf-core/star/align/main.nf            |    4 +-
 modules/nf-core/star/align/tests/main.nf.test |  523 +++-
 .../star/align/tests/main.nf.test.snap        | 2288 +++++++++++++----
 .../star/genomegenerate/environment.yml       |    2 -
 .../star/genomegenerate/tests/main.nf.test    |   39 +-
 .../genomegenerate/tests/main.nf.test.snap    |  182 +-
 .../nf-core/stringtie/merge/environment.yml   |    2 -
 .../stringtie/merge/tests/main.nf.test        |    2 +-
 .../stringtie/stringtie/environment.yml       |    2 -
 .../stringtie/stringtie/tests/main.nf.test    |  139 +-
 .../stringtie/tests/main.nf.test.snap         |  504 +++-
 .../ucsc/gtftogenepred/environment.yml        |    2 -
 .../ucsc/gtftogenepred/tests/main.nf.test     |   36 +
 .../gtftogenepred/tests/main.nf.test.snap     |   51 +
 .../ucsc/gtftogenepred/tests/nextflow.config  |    8 +
 88 files changed, 6478 insertions(+), 2021 deletions(-)
 create mode 100644 modules/nf-core/arriba/download/tests/main.nf.test
 create mode 100644 modules/nf-core/arriba/download/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/multiqc/tests/nextflow.config
 create mode 100644 modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test
 create mode 100644 modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test
 create mode 100644 modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/sort/tests/nextflow_cram.config
 create mode 100644 modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test
 create mode 100644 modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/ucsc/gtftogenepred/tests/nextflow.config

diff --git a/modules.json b/modules.json
index b3150b16b..2b34aabb8 100644
--- a/modules.json
+++ b/modules.json
@@ -7,142 +7,142 @@
                 "nf-core": {
                     "agat/convertspgff2tsv": {
                         "branch": "master",
-                        "git_sha": "b1d41826971b91f9649b46a8f3ccb226ee150c85",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "bbaeb3a3149def237b9d0acb9ab749ae98088e29",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "bedops/convert2bed": {
                         "branch": "master",
-                        "git_sha": "bbd685cd308e4d8bcf0c2caf82da3ee3c9e24157",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
-                        "git_sha": "9437e6053dccf4aafa022bfd6e7e9de67e625af8",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "4fc983ad0b30e6e32696fa7d980c76c7bfe1c03e",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
-                        "git_sha": "d3f215802f696f7993f25c759781d2db91232015",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
-                        "git_sha": "e6fe277739f5894711405af3e717b2470bd956b5",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
-                        "git_sha": "194fca815cf594646e638fa5476acbcc296f1850",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
-                        "git_sha": "6c996d7fbe0816dcbb68ce587ad5f873313682a1",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
-                        "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
-                        "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
-                        "git_sha": "20b0918591d4ba20047d7e13e5094bcceba81447",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "rrnatranscripts": {
                         "branch": "master",
-                        "git_sha": "7b17de553ee27a17478f6bf9df15acd26b1ef377",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
-                        "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
-                        "git_sha": "cb6b2b94fc40dea58f0b1e3dd095f3dd24f2ac8a",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
-                        "git_sha": "f153f1f10e1083c49935565844cccb7453021682",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "4352dbdb09ec40db71e9b172b97a01dcf5622c26",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
-                        "git_sha": "0bd7d2333a88483aa0476acea172e9f5f6dd83bb",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
-                        "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
-                        "git_sha": "5756b12b1fab0c07f05b9ad2d5d5c262b1a2c1ef",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "b1b959609bda44341120aed1766329909f54b8d0",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
-                        "git_sha": "acb0880789a6ebc2168d3b2d3d42b5bce6a62431",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/agat/convertspgff2tsv/environment.yml b/modules/nf-core/agat/convertspgff2tsv/environment.yml
index 690f50ae5..0410ee765 100644
--- a/modules/nf-core/agat/convertspgff2tsv/environment.yml
+++ b/modules/nf-core/agat/convertspgff2tsv/environment.yml
@@ -1,7 +1,5 @@
-name: agat_convertspgff2tsv
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::agat=1.4.0
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
index 4898a4a9b..6a2e8942e 100644
--- a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
+++ b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test
@@ -16,7 +16,7 @@ nextflow_process {
                 """
                 input[0] = [
                             [ id: 'test' ], // meta map
-                            file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3', checkIfExists: true)
                 ]
                 """
             }
@@ -40,7 +40,7 @@ nextflow_process {
                 """
                 input[0] = [
                             [ id: 'test' ], // meta map
-                            file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3', checkIfExists: true)
                 ]
                 """
             }
diff --git a/modules/nf-core/arriba/arriba/environment.yml b/modules/nf-core/arriba/arriba/environment.yml
index 8494c8c03..d0883a0d2 100644
--- a/modules/nf-core/arriba/arriba/environment.yml
+++ b/modules/nf-core/arriba/arriba/environment.yml
@@ -1,7 +1,5 @@
-name: arriba_arriba
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::arriba=2.4.0
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
index dc7d3bf99..d0883a0d2 100644
--- a/modules/nf-core/arriba/download/environment.yml
+++ b/modules/nf-core/arriba/download/environment.yml
@@ -1,7 +1,5 @@
-name: arriba_download
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::arriba=2.4.0
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index 7f11898bd..96a4e3362 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -7,6 +7,8 @@ process ARRIBA_DOWNLOAD {
         'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
         'biocontainers/arriba:2.4.0--h0033a41_2' }"
 
+    input:
+
     output:
     path "*"              , emit: reference
     path "versions.yml"           , emit: versions
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test b/modules/nf-core/arriba/download/tests/main.nf.test
new file mode 100644
index 000000000..cccc95db2
--- /dev/null
+++ b/modules/nf-core/arriba/download/tests/main.nf.test
@@ -0,0 +1,35 @@
+
+nextflow_process {
+
+    name "Test Process ARRIBA_DOWNLOAD"
+    script "../main.nf"
+    process "ARRIBA_DOWNLOAD"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "arriba"
+    tag "arriba/download"
+
+    test("test-arriba-download") {
+
+        when {
+            process {
+                """
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.reference[0].collect { file(it).name }.toSorted(),
+                    process.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
new file mode 100644
index 000000000..eda3f7066
--- /dev/null
+++ b/modules/nf-core/arriba/download/tests/main.nf.test.snap
@@ -0,0 +1,35 @@
+{
+    "test-arriba-download": {
+        "content": [
+            [
+                "CREDITS",
+                "RefSeq_viral_genomes_v2.4.0.fa.gz",
+                "blacklist_hg19_hs37d5_GRCh37_v2.4.0.tsv.gz",
+                "blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "blacklist_mm10_GRCm38_v2.4.0.tsv.gz",
+                "blacklist_mm39_GRCm39_v2.4.0.tsv.gz",
+                "cytobands_hg19_hs37d5_GRCh37_v2.4.0.tsv",
+                "cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "cytobands_mm10_GRCm38_v2.4.0.tsv",
+                "cytobands_mm39_GRCm39_v2.4.0.tsv",
+                "known_fusions_hg19_hs37d5_GRCh37_v2.4.0.tsv.gz",
+                "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "known_fusions_mm10_GRCm38_v2.4.0.tsv.gz",
+                "known_fusions_mm39_GRCm39_v2.4.0.tsv.gz",
+                "protein_domains_hg19_hs37d5_GRCh37_v2.4.0.gff3",
+                "protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "protein_domains_mm10_GRCm38_v2.4.0.gff3",
+                "protein_domains_mm39_GRCm39_v2.4.0.gff3",
+                "versions.yml"
+            ],
+            [
+                "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-22T17:00:38.086459"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/bedops/convert2bed/environment.yml b/modules/nf-core/bedops/convert2bed/environment.yml
index 049b29e9b..3c13066f7 100644
--- a/modules/nf-core/bedops/convert2bed/environment.yml
+++ b/modules/nf-core/bedops/convert2bed/environment.yml
@@ -1,7 +1,5 @@
-name: "bedops_convert2bed"
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - "bioconda::bedops=2.4.41"
diff --git a/modules/nf-core/bedops/convert2bed/tests/main.nf.test b/modules/nf-core/bedops/convert2bed/tests/main.nf.test
index 183f8de6c..3f2b193a6 100644
--- a/modules/nf-core/bedops/convert2bed/tests/main.nf.test
+++ b/modules/nf-core/bedops/convert2bed/tests/main.nf.test
@@ -17,7 +17,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gtf', checkIfExists: true)
                     ]
                 """
             }
@@ -41,7 +41,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'test' ], // meta map
-                    file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gtf', checkIfExists: true)
                     ]
                 """
             }
diff --git a/modules/nf-core/cat/cat/environment.yml b/modules/nf-core/cat/cat/environment.yml
index 17a04ef23..9b01c865a 100644
--- a/modules/nf-core/cat/cat/environment.yml
+++ b/modules/nf-core/cat/cat/environment.yml
@@ -1,7 +1,5 @@
-name: cat_cat
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - conda-forge::pigz=2.3.4
diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf
index adbdbd7ba..2862c64cd 100644
--- a/modules/nf-core/cat/cat/main.nf
+++ b/modules/nf-core/cat/cat/main.nf
@@ -76,4 +76,3 @@ def getFileSuffix(filename) {
     def match = filename =~ /^.*?((\.\w{1,5})?(\.\w{1,5}\.gz$))/
     return match ? match[0][1] : filename.substring(filename.lastIndexOf('.'))
 }
-
diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test
index fcee2d19f..9cb161788 100644
--- a/modules/nf-core/cat/cat/tests/main.nf.test
+++ b/modules/nf-core/cat/cat/tests/main.nf.test
@@ -29,7 +29,8 @@ nextflow_process {
         then {
             assertAll(
                 { assert !process.success },
-                { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") }
+                { assert process.stdout.toString().contains("The name of the input file can't be the same as for the output prefix") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -83,8 +84,12 @@ nextflow_process {
             def lines = path(process.out.file_out.get(0).get(1)).linesGzip
             assertAll(
                 { assert process.success },
-                { assert snapshot(lines[0..5]).match("test_cat_zipped_zipped_lines") },
-                { assert snapshot(lines.size()).match("test_cat_zipped_zipped_size")}
+                { assert snapshot(
+                    lines[0..5],
+                    lines.size(),
+                    process.out.versions
+                    ).match()
+                }
             )
         }
     }
@@ -142,8 +147,12 @@ nextflow_process {
             def lines = path(process.out.file_out.get(0).get(1)).linesGzip
             assertAll(
                 { assert process.success },
-                { assert snapshot(lines[0..5]).match("test_cat_unzipped_zipped_lines") },
-                { assert snapshot(lines.size()).match("test_cat_unzipped_zipped_size")}
+                { assert snapshot(
+                    lines[0..5],
+                    lines.size(),
+                    process.out.versions
+                    ).match()
+                }
             )
         }
     }
@@ -170,8 +179,12 @@ nextflow_process {
             def lines = path(process.out.file_out.get(0).get(1)).linesGzip
             assertAll(
                 { assert process.success },
-                { assert snapshot(lines[0..5]).match("test_cat_one_file_unzipped_zipped_lines") },
-                { assert snapshot(lines.size()).match("test_cat_one_file_unzipped_zipped_size")}
+                { assert snapshot(
+                    lines[0..5],
+                    lines.size(),
+                    process.out.versions
+                    ).match()
+                }
             )
         }
     }
diff --git a/modules/nf-core/cat/cat/tests/main.nf.test.snap b/modules/nf-core/cat/cat/tests/main.nf.test.snap
index 423571ba2..b7623ee65 100644
--- a/modules/nf-core/cat/cat/tests/main.nf.test.snap
+++ b/modules/nf-core/cat/cat/tests/main.nf.test.snap
@@ -1,10 +1,4 @@
 {
-    "test_cat_unzipped_zipped_size": {
-        "content": [
-            375
-        ],
-        "timestamp": "2023-10-16T14:33:08.049445686"
-    },
     "test_cat_unzipped_unzipped": {
         "content": [
             {
@@ -34,6 +28,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
         "timestamp": "2023-10-16T14:32:18.500464399"
     },
     "test_cat_zipped_unzipped": {
@@ -65,9 +63,13 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
         "timestamp": "2023-10-16T14:32:49.642741302"
     },
-    "test_cat_zipped_zipped_lines": {
+    "test_cat_zipped_zipped": {
         "content": [
             [
                 "MT192765.1\tGenbank\ttranscript\t259\t29667\t.\t+\t.\tID=unknown_transcript_1;geneID=orf1ab;gene_name=orf1ab",
@@ -76,11 +78,31 @@
                 "MT192765.1\tGenbank\tCDS\t13461\t21548\t.\t+\t0\tParent=unknown_transcript_1;exception=\"ribosomal slippage\";gbkey=CDS;gene=orf1ab;note=\"pp1ab;translated=by -1 ribosomal frameshift\";product=\"orf1ab polyprotein\";protein_id=QIK50426.1",
                 "MT192765.1\tGenbank\tCDS\t21556\t25377\t.\t+\t0\tParent=unknown_transcript_1;gbkey=CDS;gene=S;note=\"structural protein\";product=\"surface glycoprotein\";protein_id=QIK50427.1",
                 "MT192765.1\tGenbank\tgene\t21556\t25377\t.\t+\t.\tParent=unknown_transcript_1"
+            ],
+            78,
+            [
+                "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:51:46.802978"
+    },
+    "test_cat_name_conflict": {
+        "content": [
+            [
+                
             ]
         ],
-        "timestamp": "2023-10-16T14:32:33.629048645"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:51:29.45394"
     },
-    "test_cat_unzipped_zipped_lines": {
+    "test_cat_one_file_unzipped_zipped": {
         "content": [
             [
                 ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome",
@@ -89,11 +111,19 @@
                 "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG",
                 "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT",
                 "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG"
+            ],
+            374,
+            [
+                "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
             ]
         ],
-        "timestamp": "2023-10-16T14:33:08.038830506"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:52:02.774016"
     },
-    "test_cat_one_file_unzipped_zipped_lines": {
+    "test_cat_unzipped_zipped": {
         "content": [
             [
                 ">MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome",
@@ -102,20 +132,16 @@
                 "TAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGG",
                 "GTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTT",
                 "ACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAG"
+            ],
+            375,
+            [
+                "versions.yml:md5,115ed6177ebcff24eb99d503fa5ef894"
             ]
         ],
-        "timestamp": "2023-10-16T14:33:21.39642399"
-    },
-    "test_cat_zipped_zipped_size": {
-        "content": [
-            78
-        ],
-        "timestamp": "2023-10-16T14:32:33.641869244"
-    },
-    "test_cat_one_file_unzipped_zipped_size": {
-        "content": [
-            374
-        ],
-        "timestamp": "2023-10-16T14:33:21.4094373"
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:51:57.581523"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml
index 8c69b121f..c7eb9bd1c 100644
--- a/modules/nf-core/cat/fastq/environment.yml
+++ b/modules/nf-core/cat/fastq/environment.yml
@@ -1,7 +1,5 @@
-name: cat_fastq
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - conda-forge::coreutils=8.30
diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf
index f132b2adc..b68e5f911 100644
--- a/modules/nf-core/cat/fastq/main.nf
+++ b/modules/nf-core/cat/fastq/main.nf
@@ -53,9 +53,9 @@ process CAT_FASTQ {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def readList = reads instanceof List ? reads.collect{ it.toString() } : [reads.toString()]
     if (meta.single_end) {
-        if (readList.size > 1) {
+        if (readList.size >= 1) {
             """
-            touch ${prefix}.merged.fastq.gz
+            echo '' | gzip > ${prefix}.merged.fastq.gz
 
             cat <<-END_VERSIONS > versions.yml
             "${task.process}":
@@ -64,10 +64,10 @@ process CAT_FASTQ {
             """
         }
     } else {
-        if (readList.size > 2) {
+        if (readList.size >= 2) {
             """
-            touch ${prefix}_1.merged.fastq.gz
-            touch ${prefix}_2.merged.fastq.gz
+            echo '' | gzip > ${prefix}_1.merged.fastq.gz
+            echo '' | gzip > ${prefix}_2.merged.fastq.gz
 
             cat <<-END_VERSIONS > versions.yml
             "${task.process}":
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test b/modules/nf-core/cat/fastq/tests/main.nf.test
index a71dcb8df..f88a78b6c 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test
@@ -13,9 +13,6 @@ nextflow_process {
     test("test_cat_fastq_single_end") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([
@@ -38,9 +35,6 @@ nextflow_process {
     test("test_cat_fastq_paired_end") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([
@@ -65,9 +59,6 @@ nextflow_process {
     test("test_cat_fastq_single_end_same_name") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([
@@ -90,9 +81,6 @@ nextflow_process {
     test("test_cat_fastq_paired_end_same_name") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([
@@ -117,9 +105,129 @@ nextflow_process {
     test("test_cat_fastq_single_end_single_file") {
 
         when {
-            params {
-                outdir   = "$outputDir"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_single_end - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_paired_end - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true)]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_single_end_same_name - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_paired_end_same_name - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
+                """
             }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_single_end_single_file - stub") {
+
+        options "-stub"
+
+        when {
             process {
                 """
                 input[0] = Channel.of([
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test.snap b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
index 43dfe28fc..aec119a94 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test.snap
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
@@ -28,6 +28,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
         "timestamp": "2024-01-17T17:30:39.816981"
     },
     "test_cat_fastq_single_end_same_name": {
@@ -59,6 +63,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
         "timestamp": "2024-01-17T17:32:35.229332"
     },
     "test_cat_fastq_single_end_single_file": {
@@ -90,6 +98,10 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
         "timestamp": "2024-01-17T17:34:00.058829"
     },
     "test_cat_fastq_paired_end_same_name": {
@@ -127,8 +139,123 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
         "timestamp": "2024-01-17T17:33:33.031555"
     },
+    "test_cat_fastq_single_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T12:07:28.244999"
+    },
+    "test_cat_fastq_paired_end_same_name - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T12:07:57.070911"
+    },
+    "test_cat_fastq_single_end_same_name - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T12:07:46.796254"
+    },
     "test_cat_fastq_paired_end": {
         "content": [
             {
@@ -164,6 +291,86 @@
                 ]
             }
         ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
         "timestamp": "2024-01-17T17:32:02.270935"
+    },
+    "test_cat_fastq_paired_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T12:07:37.807553"
+    },
+    "test_cat_fastq_single_end_single_file - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T12:14:51.861264"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/fastp/environment.yml
index 70389e664..26d4aca5d 100644
--- a/modules/nf-core/fastp/environment.yml
+++ b/modules/nf-core/fastp/environment.yml
@@ -1,7 +1,5 @@
-name: fastp
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::fastp=0.23.4
diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf
index 4fc19b744..e1b9f5656 100644
--- a/modules/nf-core/fastp/main.nf
+++ b/modules/nf-core/fastp/main.nf
@@ -10,6 +10,7 @@ process FASTP {
     input:
     tuple val(meta), path(reads)
     path  adapter_fasta
+    val   discard_trimmed_pass
     val   save_trimmed_fail
     val   save_merged
 
@@ -18,9 +19,9 @@ process FASTP {
     tuple val(meta), path('*.json')           , emit: json
     tuple val(meta), path('*.html')           , emit: html
     tuple val(meta), path('*.log')            , emit: log
-    path "versions.yml"                       , emit: versions
     tuple val(meta), path('*.fail.fastq.gz')  , optional:true, emit: reads_fail
     tuple val(meta), path('*.merged.fastq.gz'), optional:true, emit: reads_merged
+    path "versions.yml"                       , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -30,6 +31,8 @@ process FASTP {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : ""
     def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--failed_out ${prefix}.paired.fail.fastq.gz --unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
+    def out_fq1 = discard_trimmed_pass ?: ( meta.single_end ? "--out1 ${prefix}.fastp.fastq.gz" : "--out1 ${prefix}_1.fastp.fastq.gz" )
+    def out_fq2 = discard_trimmed_pass ?: "--out2 ${prefix}_2.fastp.fastq.gz"
     // Added soft-links to original fastqs for consistent naming in MultiQC
     // Use single ended for interleaved. Add --interleaved_in in config.
     if ( task.ext.args?.contains('--interleaved_in') ) {
@@ -59,7 +62,7 @@ process FASTP {
 
         fastp \\
             --in1 ${prefix}.fastq.gz \\
-            --out1  ${prefix}.fastp.fastq.gz \\
+            $out_fq1 \\
             --thread $task.cpus \\
             --json ${prefix}.fastp.json \\
             --html ${prefix}.fastp.html \\
@@ -81,8 +84,8 @@ process FASTP {
         fastp \\
             --in1 ${prefix}_1.fastq.gz \\
             --in2 ${prefix}_2.fastq.gz \\
-            --out1 ${prefix}_1.fastp.fastq.gz \\
-            --out2 ${prefix}_2.fastp.fastq.gz \\
+            $out_fq1 \\
+            $out_fq2 \\
             --json ${prefix}.fastp.json \\
             --html ${prefix}.fastp.html \\
             $adapter_list \\
@@ -103,14 +106,16 @@ process FASTP {
     stub:
     def prefix              = task.ext.prefix ?: "${meta.id}"
     def is_single_output    = task.ext.args?.contains('--interleaved_in') || meta.single_end
-    def touch_reads         = is_single_output ? "${prefix}.fastp.fastq.gz" : "${prefix}_1.fastp.fastq.gz ${prefix}_2.fastp.fastq.gz"
-    def touch_merged        = (!is_single_output && save_merged) ? "touch ${prefix}.merged.fastq.gz" : ""
+    def touch_reads         = (discard_trimmed_pass) ? "" : (is_single_output) ? "echo '' | gzip > ${prefix}.fastp.fastq.gz" : "echo '' | gzip > ${prefix}_1.fastp.fastq.gz ; echo '' | gzip > ${prefix}_2.fastp.fastq.gz"
+    def touch_merged        = (!is_single_output && save_merged) ? "echo '' | gzip >  ${prefix}.merged.fastq.gz" : ""
+    def touch_fail_fastq    = (!save_trimmed_fail) ? "" : meta.single_end ? "echo '' | gzip > ${prefix}.fail.fastq.gz" : "echo '' | gzip > ${prefix}.paired.fail.fastq.gz ; echo '' | gzip > ${prefix}_1.fail.fastq.gz ; echo '' | gzip > ${prefix}_2.fail.fastq.gz"
     """
-    touch $touch_reads
+    $touch_reads
+    $touch_fail_fastq
+    $touch_merged
     touch "${prefix}.fastp.json"
     touch "${prefix}.fastp.html"
     touch "${prefix}.fastp.log"
-    $touch_merged
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml
index c22a16abd..8dfecc184 100644
--- a/modules/nf-core/fastp/meta.yml
+++ b/modules/nf-core/fastp/meta.yml
@@ -27,12 +27,16 @@ input:
       type: file
       description: File in FASTA format containing possible adapters to remove.
       pattern: "*.{fasta,fna,fas,fa}"
+  - discard_trimmed_pass:
+      type: boolean
+      description: Specify true to not write any reads that pass trimming thresholds. |
+        This can be used to use fastp for the output report only.
   - save_trimmed_fail:
       type: boolean
       description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz`
   - save_merged:
       type: boolean
-      description: Specify true to save all merged reads to the a file ending in `*.merged.fastq.gz`
+      description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz`
 output:
   - meta:
       type: map
diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test
index 6f1f48978..30dbb8aab 100644
--- a/modules/nf-core/fastp/tests/main.nf.test
+++ b/modules/nf-core/fastp/tests/main.nf.test
@@ -10,221 +10,290 @@ nextflow_process {
     test("test_fastp_single_end") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
+
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = false
-                save_merged       = false
-
                 input[0] = Channel.of([
                     [ id:'test', single_end:true ],
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = false
                 """
             }
         }
 
         then {
-            def html_text  = [ "Q20 bases:</td><td class='col2'>12.922000 K (92.984097%)",
-                                "single end (151 cycles)" ]
-            def log_text   = [ "Q20 bases: 12922(92.9841%)",
-								"reads passed filter: 99" ]
-            def read_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
-								"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
-								"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
-								"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/1",
-								"ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGC",
-								"AAA6AEEEEEEEEEAEEE/6EEAEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEE<AAEEEEEEEEEEE</EEEA/AE"]
             assertAll(
                 { assert process.success },
-                { read_lines.each { read_line ->
-                    { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) }
-                    }
-                },
-                { html_text.each { html_part ->
-                    { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) }
-                    }
-                },
-                { assert snapshot(process.out.json).match("test_fastp_single_end_json") },
-                { log_text.each { log_part ->
-                    { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
-                    }
-                },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { file(it[1]).getName() } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_single_end-_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_single_end") }
+                { assert path(process.out.html.get(0).get(1)).getText().contains("single end (151 cycles)") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("reads passed filter: 99") },
+                { assert snapshot(
+                    process.out.json,
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.versions).match() }
             )
         }
     }
 
-    test("test_fastp_single_end-stub") {
-
-        options '-stub'
+    test("test_fastp_paired_end") {
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
+
             process {
                 """
                 adapter_fasta     = []
+                save_trimmed_pass = true
                 save_trimmed_fail = false
                 save_merged       = false
 
                 input[0] = Channel.of([
-                    [ id:'test', single_end:true ],
-                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = false
                 """
             }
         }
 
         then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.html.get(0).get(1)).getText().contains("The input has little adapter percentage (~0.000000%), probably it's trimmed before.") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("Q30 bases: 12281(88.3716%)") },
+                { assert snapshot(
+                    process.out.json,
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.versions).match() }
+            )
+        }
+    }
 
+    test("fastp test_fastp_interleaved") {
+
+        config './nextflow.interleaved.config'
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
+
+        then {
             assertAll(
                 { assert process.success },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { file(it[1]).getName() } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_single_end-for_stub_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_single_end_stub") }
+                { assert path(process.out.html.get(0).get(1)).getText().contains("paired end (151 cycles + 151 cycles)") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("reads passed filter: 162") },
+                { assert process.out.reads_fail == [] },
+                { assert process.out.reads_merged == [] },
+                { assert snapshot(
+                    process.out.reads,
+                    process.out.json,
+                    process.out.versions).match() }
             )
         }
     }
 
-    test("test_fastp_paired_end") {
+    test("test_fastp_single_end_trim_fail") {
 
         when {
-            params {
-                outdir   = "$outputDir"
+
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = []
+                input[2] = false
+                input[3] = true
+                input[4] = false
+                """
             }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.html.get(0).get(1)).getText().contains("single end (151 cycles)") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("reads passed filter: 99") },
+                { assert snapshot(
+                    process.out.json,
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_fastp_paired_end_trim_fail") {
+
+        config './nextflow.save_failed.config'
+        when {
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = false
-                save_merged       = false
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
+                ])
+                input[1] = []
+                input[2] = false
+                input[3] = true
+                input[4] = false
+                """
+            }
+        }
 
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.html.get(0).get(1)).getText().contains("The input has little adapter percentage (~0.000000%), probably it's trimmed before.") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("reads passed filter: 162") },
+                { assert snapshot(
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.json,
+                    process.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_fastp_paired_end_merged") {
+
+        when {
+            process {
+                """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = true
                 """
             }
         }
 
         then {
-            def html_text   = [ "Q20 bases:</td><td class='col2'>25.719000 K (93.033098%)",
-									"The input has little adapter percentage (~0.000000%), probably it's trimmed before."]
-            def log_text    = [ "No adapter detected for read1",
-									"Q30 bases: 12281(88.3716%)"]
-            def json_text   = ['"passed_filter_reads": 198']
-            def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
-									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
-									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
-									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/1",
-									"ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGC",
-									"AAA6AEEEEEEEEEAEEE/6EEAEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEE<AAEEEEEEEEEEE</EEEA/AE"]
-            def read2_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/2",
-									"ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTG",
-									"AAAAAEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEAEEEEEAAEEEEEEEEEAAEAAA<<EAAEEEEEEEAAA<<<AE",
-									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/2",
-									"GCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGT",
-									"AAAAA6EEAEEEEEAEEAEEAEEEEEEA6EEEEAEEAEEEEE6EEEEEEAEEEEA///A<<EEEEEEEEEAEEEEEE"]
             assertAll(
                 { assert process.success },
-                { read1_lines.each { read1_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) }
-                    }
-                },
-                { read2_lines.each { read2_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) }
-                    }
-                },
-                { html_text.each { html_part ->
-                    { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) }
-                    }
-                },
-                { json_text.each { json_part ->
-                    { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) }
-                    }
-                },
-                { log_text.each { log_part ->
-                    { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
-                    }
-                },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_paired_end_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_paired_end") }
+                { assert path(process.out.html.get(0).get(1)).getText().contains("The input has little adapter percentage (~0.000000%), probably it's trimmed before.") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("total reads: 75") },
+                { assert snapshot(
+                    process.out.json,
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.versions).match() },
             )
         }
     }
 
-    test("test_fastp_paired_end-stub") {
-
-        options '-stub'
+    test("test_fastp_paired_end_merged_adapterlist") {
 
         when {
-            params {
-                outdir   = "$outputDir"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] =  Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ])
+                input[2] = false
+                input[3] = false
+                input[4] = true
+                """
             }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.html.get(0).get(1)).getText().contains("<div id='After_filtering__merged__quality'>") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("total bases: 13683") },
+                { assert snapshot(
+                    process.out.json,
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_fastp_single_end_qc_only") {
+
+        when {
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = false
-                save_merged       = false
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = []
+                input[2] = true
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.html.get(0).get(1)).getText().contains("single end (151 cycles)") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("reads passed filter: 99") },
+                { assert snapshot(
+                    process.out.json,
+                    process.out.reads,
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.reads_merged,
+                    process.out.versions).match() }
+            )
+        }
+    }
 
+    test("test_fastp_paired_end_qc_only") {
+
+        when {
+            process {
+                """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = true
+                input[3] = false
+                input[4] = false
                 """
             }
         }
@@ -232,114 +301,99 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_paired_end-for_stub_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_paired_end-stub") }
+                { assert path(process.out.html.get(0).get(1)).getText().contains("The input has little adapter percentage (~0.000000%), probably it's trimmed before.") },
+                { assert path(process.out.log.get(0).get(1)).getText().contains("Q30 bases: 12281(88.3716%)") },
+                { assert snapshot(
+                    process.out.json,
+                    process.out.reads,
+                    process.out.reads,
+                    process.out.reads_fail,
+                    process.out.reads_fail,
+                    process.out.reads_merged,
+                    process.out.reads_merged,
+                    process.out.versions).match() }
             )
         }
     }
 
-    test("fastp test_fastp_interleaved") {
+    test("test_fastp_single_end - stub") {
+
+        options "-stub"
 
-        config './nextflow.interleaved.config'
         when {
-            params {
-                outdir   = "$outputDir"
+
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = false
+                """
             }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_fastp_paired_end - stub") {
+
+        options "-stub"
+
+        when {
+
             process {
                 """
                 adapter_fasta     = []
+                save_trimmed_pass = true
                 save_trimmed_fail = false
                 save_merged       = false
 
                 input[0] = Channel.of([
-                    [ id:'test', single_end:true ], // meta map
-                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = false
                 """
             }
         }
 
         then {
-            def html_text  = [ "Q20 bases:</td><td class='col2'>25.719000 K (93.033098%)",
-									"paired end (151 cycles + 151 cycles)"]
-            def log_text   = [ "Q20 bases: 12922(92.9841%)",
-									"reads passed filter: 162"]
-            def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
-									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
-									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
-									"@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/2",
-									"ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTG",
-									"AAAAAEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEAEEEEEAAEEEEEEEEEAAEAAA<<EAAEEEEEEEAAA<<<AE"]
             assertAll(
                 { assert process.success },
-                { read_lines.each { read_line ->
-                    { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) }
-                    }
-                },
-                { html_text.each { html_part ->
-                    { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) }
-                    }
-                },
-                { assert snapshot(process.out.json).match("fastp test_fastp_interleaved_json") },
-                { log_text.each { log_part ->
-                    { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
-                    }
-                },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { file(it[1]).getName() } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_interleaved-_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_interleaved") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("fastp test_fastp_interleaved-stub") {
+    test("fastp - stub test_fastp_interleaved") {
 
-        options '-stub'
+        options "-stub"
 
         config './nextflow.interleaved.config'
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = false
-                save_merged       = false
-
                 input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = false
                 """
             }
         }
@@ -347,277 +401,112 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { file(it[1]).getName() } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_interleaved-for_stub_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_interleaved-stub") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("test_fastp_single_end_trim_fail") {
+    test("test_fastp_single_end_trim_fail - stub") {
+
+        options "-stub"
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
+
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = true
-                save_merged       = false
-
                 input[0] = Channel.of([
                     [ id:'test', single_end:true ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = true
+                input[4] = false
                 """
             }
         }
 
         then {
-            def html_text         = [ "Q20 bases:</td><td class='col2'>12.922000 K (92.984097%)",
-									"single end (151 cycles)"]
-            def log_text          = [ "Q20 bases: 12922(92.9841%)",
-									"reads passed filter: 99" ]
-            def read_lines        = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
-									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
-									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
-									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/1",
-									"ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGC",
-									"AAA6AEEEEEEEEEAEEE/6EEAEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEE<AAEEEEEEEEEEE</EEEA/AE"]
-            def failed_read_lines = [ "@ERR5069949.885966 NS500628:121:HK3MMAFX2:4:11610:19682:20132/1 failed_quality_filter",
-									"GTCTAATCATAATTTCTTGGTACAGGCTGGTATTGTTCATCTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTT",
-									"AAA//E/EAA/E//E//E//E/E//AE/A/E//EAEA///AE//E///E/EEE6EEEAEEA///E/AEE/EAEE/E//E"]
             assertAll(
                 { assert process.success },
-                { read_lines.each { read_line ->
-                    { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) }
-                    }
-                },
-                { failed_read_lines.each { failed_read_line ->
-                    { assert path(process.out.reads_fail.get(0).get(1)).linesGzip.contains(failed_read_line) }
-                    }
-                },
-                { html_text.each { html_part ->
-                    { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) }
-                    }
-                },
-                { assert snapshot(process.out.json).match("test_fastp_single_end_trim_fail_json") },
-                { log_text.each { log_part ->
-                    { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
-                    }
-                },
-                { assert snapshot(process.out.versions).match("versions_single_end_trim_fail") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("test_fastp_paired_end_trim_fail") {
+    test("test_fastp_paired_end_trim_fail - stub") {
+
+        options "-stub"
 
         config './nextflow.save_failed.config'
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = true
-                save_merged       = false
-
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = true
+                input[4] = false
                 """
             }
         }
 
         then {
-            def html_text           = [ "Q20 bases:</td><td class='col2'>25.719000 K (93.033098%)",
-									"The input has little adapter percentage (~0.000000%), probably it's trimmed before."]
-            def log_text            = [ "No adapter detected for read1",
-									"Q30 bases: 12281(88.3716%)"]
-            def json_text           = ['"passed_filter_reads": 162']
-            def read1_lines         = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1",
-									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT",
-									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAA",
-									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/1",
-									"ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGC",
-									"AAA6AEEEEEEEEEAEEE/6EEAEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEE<AAEEEEEEEEEEE</EEEA/AE"]
-            def read2_lines         = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/2",
-									"ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTG",
-									"AAAAAEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEAEEEEEAAEEEEEEEEEAAEAAA<<EAAEEEEEEEAAA<<<AE",
-									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/2",
-									"GCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGT",
-									"AAAAA6EEAEEEEEAEEAEEAEEEEEEA6EEEEAEEAEEEEE6EEEEEEAEEEEA///A<<EEEEEEEEEAEEEEEE"]
-            def failed_read2_lines  = ["@ERR5069949.973930 NS500628:121:HK3MMAFX2:2:21105:5548:5659/2",
-									"CCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAG",
-									"AAAAAEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEEEEEEAE<EAEEEEEEAEEAEE"]
-            // def failed_read1_lines = path(process.out.reads_fail.get(0).get(1).get(0)).linesGzip is empty file
             assertAll(
                 { assert process.success },
-                { assert path(process.out.reads_fail.get(0).get(1).get(0)).exists() },
-                { read1_lines.each { read1_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) }
-                    }
-                },
-                { read2_lines.each { read2_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) }
-                    }
-                },
-                { failed_read2_lines.each { failed_read2_line ->
-                    { assert path(process.out.reads_fail.get(0).get(1).get(2)).linesGzip.contains(failed_read2_line) }
-                    }
-                },
-                { html_text.each { html_part ->
-                    { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) }
-                    }
-                },
-                { json_text.each { json_part ->
-                    { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) }
-                    }
-                },
-                { log_text.each { log_part ->
-                    { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
-                    }
-                },
-                { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("test_fastp_paired_end_merged") {
+    test("test_fastp_paired_end_merged - stub") {
+
+        options "-stub"
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = false
-                save_merged       = true
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = false
+                input[3] = false
+                input[4] = true
                 """
             }
         }
 
         then {
-            def html_text         = [ "<div id='After_filtering__merged__quality'>"]
-            def log_text          = [ "Merged and filtered:",
-									"total reads: 75",
-									"total bases: 13683"]
-            def json_text         = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683']
-            def read1_lines       = [ "@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1",
-									"CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC",
-									"AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE<AEEAEEEAEAEEEAEAEEAE/AEEEEAEEEEAEA",
-									"@ERR5069949.324865 NS500628:121:HK3MMAFX2:1:11102:17526:14721/1",
-									"CACAAACTCTAAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCAGATAATAATGTTGCAAGTAGAACTTCGTGCAGATTAAAATTTTCATAAGCACTCTAAAGAAGTTGAATGTCTTCAAATTTCTTAACATTAGGGCCCACAACAAG",
-									"AAAAAEA/A<EAA/AE/EE/EE//////EA/EEE/E/EEEE//E/6//EA//<AA/A/EEEAA/EEEE/EEEA/E/</AEE////AEEEE//<E//EAE/A///<EEE//<E<<EEE<///A//E/E/EEEAA/<A////<A/AEAAA//E"]
-            def read2_lines       = ["@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/2",
-									"GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTGAGTAGTTAGAAG",
-									"AAAAAEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEA/EAA</EEEEEEEEEE/AE//A/E<AE<AA<AEEE/AAEAAAEEAEEA<A6AEEA<EEAEEEEEEEAA//EE",
-									"@ERR5069949.324865 NS500628:121:HK3MMAFX2:1:11102:17526:14721/2",
-									"ATGAATCTGATGAATACATAGCTACTAATGGACCTCTTAAAGTGCGTGGTAGTTGTGATTAAAGCGGACACATACTTGCTAAACACTCTCTTCATGATGTC",
-									"A/AAAEEEEA6AA6EE//EEA/EEEAE/EA/A////E</EEAA//EEA////EAE<///E/AEA</AAE/EA//E<EAAAE/AA//AEE//A/AE//</EE"]
-            def read_merged_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1 merged_150_37",
-									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGTGGTGCAGGTAATTGAGCAGGGTCGCCAATGTACACAT",
-									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAAEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEAAAAA",
-									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/1 merged_77_0",
-									"ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGC",
-									"AAA6AEEEEEEEEEAEEE/6EEAEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEE<AAEEEEEEEEEEE</EEEA/AE"]
             assertAll(
                 { assert process.success },
-                { read1_lines.each { read1_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) }
-                    }
-                },
-                { read2_lines.each { read2_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) }
-                    }
-                },
-                { read_merged_lines.each { read_merged_line ->
-                    { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) }
-                    }
-                },
-                { html_text.each { html_part ->
-                    { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) }
-                    }
-                },
-                { json_text.each { json_part ->
-                    { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) }
-                    }
-                },
-                { log_text.each { log_part ->
-                    { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
-                    }
-                },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_paired_end_merged_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_paired_end_merged") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("test_fastp_paired_end_merged-stub") {
+    test("test_fastp_paired_end_merged_adapterlist - stub") {
 
-        options '-stub'
+        options "-stub"
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                adapter_fasta     = []
-                save_trimmed_fail = false
-                save_merged       = true
-
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] =  Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ])
+                input[2] = false
+                input[3] = false
+                input[4] = true
                 """
             }
         }
@@ -625,101 +514,63 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                {
-                    assert snapshot(
-                        (
-                            [process.out.reads[0][0].toString()] + // meta
-                            process.out.reads.collect { it[1].collect { item -> file(item).getName() } } +
-                            process.out.json.collect { file(it[1]).getName() } +
-                            process.out.html.collect { file(it[1]).getName() } +
-                            process.out.log.collect { file(it[1]).getName() } +
-                            process.out.reads_fail.collect { file(it[1]).getName() } +
-                            process.out.reads_merged.collect { file(it[1]).getName() }
-                        ).sort()
-                    ).match("test_fastp_paired_end_merged-for_stub_match")
-                },
-                { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("test_fastp_paired_end_merged_adapterlist") {
+    test("test_fastp_single_end_qc_only - stub") {
+
+        options "-stub"
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
-                adapter_fasta     = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ])
-                save_trimmed_fail = false
-                save_merged       = true
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = []
+                input[2] = true
+                input[3] = false
+                input[4] = false
+                """
+            }
+        }
 
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_fastp_paired_end_qc_only - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
                     [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = adapter_fasta
-                input[2] = save_trimmed_fail
-                input[3] = save_merged
+                input[1] = []
+                input[2] = true
+                input[3] = false
+                input[4] = false
                 """
             }
         }
 
         then {
-            def html_text         = [ "<div id='After_filtering__merged__quality'>"]
-            def log_text          = [ "Merged and filtered:",
-									"total reads: 75",
-									"total bases: 13683"]
-            def json_text         = ['"merged_and_filtered": {', '"total_reads": 75', '"total_bases": 13683',"--adapter_fasta"]
-            def read1_lines       = ["@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/1",
-									"CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC",
-									"AAAAAEAEEAEEEEEEEEEEEEEEEEAEEEEAEEEEEEEEAEEEEEEEEEEEEEEEEE/EAEEEEEE/6EEEEEEEEEEAEEAEEE/EE/AEEAEEEEEAEEEA/EEAAEAE<AEEAEEEAEAEEEAEAEEAE/AEEEEAEEEEAEA",
-									"@ERR5069949.324865 NS500628:121:HK3MMAFX2:1:11102:17526:14721/1",
-									"CACAAACTCTAAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCAGATAATAATGTTGCAAGTAGAACTTCGTGCAGATTAAAATTTTCATAAGCACTCTAAAGAAGTTGAATGTCTTCAAATTTCTTAACATTAGGGCCCACAACAAG",
-									"AAAAAEA/A<EAA/AE/EE/EE//////EA/EEE/E/EEEE//E/6//EA//<AA/A/EEEAA/EEEE/EEEA/E/</AEE////AEEEE//<E//EAE/A///<EEE//<E<<EEE<///A//E/E/EEEAA/<A////<A/AEAAA//E"]
-            def read2_lines       = ["@ERR5069949.1066259 NS500628:121:HK3MMAFX2:1:11312:18369:8333/2",
-									"GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTGAGTAGTTAGAAG",
-									"AAAAAEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEA/EAA</EEEEEEEEEE/AE//A/E<AE<AA<AEEE/AAEAAAEEAEEA<A6AEEA<EEAEEEEEEEAA//EE",
-									"@ERR5069949.324865 NS500628:121:HK3MMAFX2:1:11102:17526:14721/2",
-									"ATGAATCTGATGAATACATAGCTACTAATGGACCTCTTAAAGTGCGTGGTAGTTGTGATTAAAGCGGACACATACTTGCTAAACACTCTCTTCATGATGTC",
-									"A/AAAEEEEA6AA6EE//EEA/EEEAE/EA/A////E</EEAA//EEA////EAE<///E/AEA</AAE/EA//E<EAAAE/AA//AEE//A/AE//</EE"]
-            def read_merged_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1 merged_150_37",
-									"TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGTGGTGCAGGTAATTGAGCAGGGTCGCCAATGTACACAT",
-									"AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE<EEAAAEEEEEEEEEAAAAEAEEEAEEEEEE<AAAAEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEAAAAA",
-									"@ERR5069949.576388 NS500628:121:HK3MMAFX2:4:11501:11167:14939/1 merged_77_0",
-									"ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGC",
-									"AAA6AEEEEEEEEEAEEE/6EEAEEEAEEEEEAEEEEEEEEEEEEEEEEEEEEE<AAEEEEEEEEEEE</EEEA/AE"]
-
             assertAll(
                 { assert process.success },
-                { read1_lines.each { read1_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) }
-                    }
-                },
-                { read2_lines.each { read2_line ->
-                    { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) }
-                    }
-                },
-                { read_merged_lines.each { read_merged_line ->
-                    { assert path(process.out.reads_merged.get(0).get(1)).linesGzip.contains(read_merged_line) }
-                    }
-                },
-                { html_text.each { html_part ->
-                    { assert path(process.out.html.get(0).get(1)).getText().contains(html_part) }
-                    }
-                },
-                { json_text.each { json_part ->
-                    { assert path(process.out.json.get(0).get(1)).getText().contains(json_part) }
-                    }
-                },
-                { log_text.each { log_part ->
-                    { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) }
-                    }
-                },
-                { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap
index 3e8762889..54be7e45f 100644
--- a/modules/nf-core/fastp/tests/main.nf.test.snap
+++ b/modules/nf-core/fastp/tests/main.nf.test.snap
@@ -1,55 +1,178 @@
 {
-    "fastp test_fastp_interleaved_json": {
+    "test_fastp_single_end_qc_only - stub": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.fastp.json:md5,b24e0624df5cc0b11cd5ba21b726fb22"
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "json": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    
+                ],
+                "reads_fail": [
+                    
+                ],
+                "reads_merged": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
                 ]
-            ]
+            }
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-03-18T16:19:15.063001"
+        "timestamp": "2024-07-05T14:31:10.841098"
     },
-    "test_fastp_paired_end_merged-for_stub_match": {
+    "test_fastp_paired_end": {
         "content": [
             [
                 [
-                    "test_1.fastp.fastq.gz",
-                    "test_2.fastp.fastq.gz"
-                ],
-                "test.fastp.html",
-                "test.fastp.json",
-                "test.fastp.log",
-                "test.merged.fastq.gz",
-                "{id=test, single_end=false}"
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "test_1.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7",
+                        "test_2.fastp.fastq.gz:md5,25cbdca08e2083dbd4f0502de6b62f39"
+                    ]
+                ]
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-01-17T18:10:13.467574"
+        "timestamp": "2024-07-05T13:43:28.665779"
     },
-    "versions_interleaved": {
+    "test_fastp_paired_end_merged_adapterlist": {
         "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.fastp.json:md5,5914ca3f21ce162123a824e33e8564f6"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "test_1.fastp.fastq.gz:md5,54b726a55e992a869fd3fa778afe1672",
+                        "test_2.fastp.fastq.gz:md5,29d3b33b869f7b63417b8ff07bb128ba"
+                    ]
+                ]
+            ],
+            [
+                
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.merged.fastq.gz:md5,c873bb1ab3fa859dcc47306465e749d5"
+                ]
+            ],
             [
                 "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-01T11:56:24.615634793"
+        "timestamp": "2024-07-05T13:44:18.210375"
     },
-    "test_fastp_single_end_json": {
+    "test_fastp_single_end_qc_only": {
         "content": [
             [
                 [
@@ -57,274 +180,1152 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc"
+                    "test.fastp.json:md5,5cc5f01e449309e0e689ed6f51a2294a"
                 ]
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-03-18T16:18:43.526412"
-    },
-    "versions_paired_end": {
-        "content": [
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
             [
                 "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-01T11:55:42.333545689"
+        "timestamp": "2024-07-05T13:44:27.380974"
     },
-    "test_fastp_paired_end_match": {
+    "test_fastp_paired_end_trim_fail": {
         "content": [
             [
                 [
-                    "test_1.fastp.fastq.gz",
-                    "test_2.fastp.fastq.gz"
-                ],
-                "test.fastp.html",
-                "test.fastp.json",
-                "test.fastp.log",
-                "{id=test, single_end=false}"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-01T12:03:06.431833729"
-    },
-    "test_fastp_interleaved-_match": {
-        "content": [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "test_1.fastp.fastq.gz:md5,6ff32a64c5188b9a9192be1398c262c7",
+                        "test_2.fastp.fastq.gz:md5,db0cb7c9977e94ac2b4b446ebd017a8a"
+                    ]
+                ]
+            ],
             [
-                "test.fastp.fastq.gz",
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-                "test.fastp.html",
-                "test.fastp.json",
-                "test.fastp.log",
-                "{id=test, single_end=true}"
-            ]
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "json": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "reads_fail": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "reads_merged": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                ]
+            }
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-01-17T18:06:00.244202"
+        "timestamp": "2024-07-05T14:05:36.898142"
     },
-    "test_fastp_single_end-_match": {
+    "test_fastp_paired_end_trim_fail - stub": {
         "content": [
-            [
-                "test.fastp.fastq.gz",
-                "test.fastp.html",
-                "test.fastp.json",
-                "test.fastp.log",
-                "{id=test, single_end=true}"
-            ]
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.paired.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_1.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "json": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "reads_fail": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.paired.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_1.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fail.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "reads_merged": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                ]
+            }
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-03-18T16:18:43.580336"
+        "timestamp": "2024-07-05T14:05:49.212847"
     },
-    "versions_paired_end_merged_adapterlist": {
+    "fastp test_fastp_interleaved": {
         "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.fastp.fastq.gz:md5,217d62dc13a23e92513a1bd8e1bcea39"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.fastp.json:md5,b24e0624df5cc0b11cd5ba21b726fb22"
+                ]
+            ],
             [
                 "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-01T12:05:37.845370554"
+        "timestamp": "2024-07-05T13:43:38.910832"
     },
-    "versions_paired_end_merged": {
+    "test_fastp_single_end_trim_fail": {
         "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.fail.fastq.gz:md5,3e4aaadb66a5b8fc9b881bf39c227abd"
+                ]
+            ],
+            [
+                
+            ],
             [
                 "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-01T11:59:32.860543858"
+        "timestamp": "2024-07-05T13:43:48.22378"
     },
-    "test_fastp_single_end_trim_fail_json": {
+    "test_fastp_paired_end_qc_only": {
         "content": [
             [
                 [
                     {
                         "id": "test",
-                        "single_end": true
+                        "single_end": false
                     },
-                    "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5"
+                    "test.fastp.json:md5,623064a45912dac6f2b64e3f2e9901df"
                 ]
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-05T13:44:36.334938"
+    },
+    "test_fastp_paired_end_qc_only - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "json": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    
+                ],
+                "reads_fail": [
+                    
+                ],
+                "reads_merged": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-01-17T18:08:41.942317"
+        "timestamp": "2024-07-05T14:31:27.096468"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
index 1787b38a9..691d4c763 100644
--- a/modules/nf-core/fastqc/environment.yml
+++ b/modules/nf-core/fastqc/environment.yml
@@ -1,7 +1,5 @@
-name: fastqc
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::fastqc=0.12.1
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index d79f1c862..d8989f481 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -26,7 +26,10 @@ process FASTQC {
     def rename_to = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
 
-    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB')
+    // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
+    // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
+    // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
+    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
 
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index 70edae4d9..e9d79a074 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -23,17 +23,14 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
-            // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
-            // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
-
-            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_single") }
+                { assert process.success },
+                // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
+                // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
+                // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -54,16 +51,14 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
-            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
-            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
-            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
-            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_paired") }
+                { assert process.success },
+                { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+                { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+                { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+                { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+                { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -83,13 +78,11 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") }
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -109,13 +102,11 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_bam") }
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -138,22 +129,20 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
-            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
-            { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
-            { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
-            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
-            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
-            { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
-            { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
-            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_multiple") }
+                { assert process.success },
+                { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+                { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+                { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
+                { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
+                { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+                { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+                { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
+                { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
+                { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -173,21 +162,18 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") }
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
 
     test("sarscov2 single-end [fastq] - stub") {
 
-        options "-stub"
-
+    options "-stub"
         when {
             process {
                 """
@@ -201,12 +187,123 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-            { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
-                                process.out.zip.collect { file(it[1]).getName() } +
-                                process.out.versions ).match("fastqc_stub") }
+                { assert process.success },
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
+    test("sarscov2 paired-end [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true)
+                ])
+            """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [bam] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 multiple [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'mysample', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
 }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 86f7c3115..d5db3092f 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,88 +1,392 @@
 {
-    "fastqc_versions_interleaved": {
+    "sarscov2 custom_prefix": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:40:07.293713"
+        "timestamp": "2024-07-22T11:02:16.374038"
     },
-    "fastqc_stub": {
+    "sarscov2 single-end [fastq] - stub": {
         "content": [
-            [
-                "test.html",
-                "test.zip",
-                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
-            ]
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:24.993809"
+    },
+    "sarscov2 custom_prefix - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:31:01.425198"
+        "timestamp": "2024-07-22T11:03:10.93942"
     },
-    "fastqc_versions_multiple": {
+    "sarscov2 interleaved [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:40:55.797907"
+        "timestamp": "2024-07-22T11:01:42.355718"
     },
-    "fastqc_versions_bam": {
+    "sarscov2 paired-end [bam]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:40:26.795862"
+        "timestamp": "2024-07-22T11:01:53.276274"
     },
-    "fastqc_versions_single": {
+    "sarscov2 multiple [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:39:27.043675"
+        "timestamp": "2024-07-22T11:02:05.527626"
     },
-    "fastqc_versions_paired": {
+    "sarscov2 paired-end [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:31.188871"
+    },
+    "sarscov2 paired-end [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:34.273566"
+    },
+    "sarscov2 multiple [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:39:47.584191"
+        "timestamp": "2024-07-22T11:03:02.304411"
     },
-    "fastqc_versions_custom_prefix": {
+    "sarscov2 single-end [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:19.095607"
+    },
+    "sarscov2 interleaved [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:44.640184"
+    },
+    "sarscov2 paired-end [bam] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:41:14.576531"
+        "timestamp": "2024-07-22T11:02:53.550742"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/gatk4/bedtointervallist/environment.yml b/modules/nf-core/gatk4/bedtointervallist/environment.yml
index d6fbe2e77..55993f440 100644
--- a/modules/nf-core/gatk4/bedtointervallist/environment.yml
+++ b/modules/nf-core/gatk4/bedtointervallist/environment.yml
@@ -1,7 +1,5 @@
-name: gatk4_bedtointervallist
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::gatk4=4.5.0.0
diff --git a/modules/nf-core/gatk4/createsequencedictionary/environment.yml b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
index 78822ad03..55993f440 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/environment.yml
+++ b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
@@ -1,7 +1,5 @@
-name: gatk4_createsequencedictionary
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::gatk4=4.5.0.0
diff --git a/modules/nf-core/gatk4/markduplicates/environment.yml b/modules/nf-core/gatk4/markduplicates/environment.yml
index 7362eea6f..9fe34eb36 100644
--- a/modules/nf-core/gatk4/markduplicates/environment.yml
+++ b/modules/nf-core/gatk4/markduplicates/environment.yml
@@ -1,8 +1,6 @@
-name: gatk4_markduplicates
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::gatk4=4.5.0.0
   - bioconda::samtools=1.19.2
diff --git a/modules/nf-core/gffread/environment.yml b/modules/nf-core/gffread/environment.yml
index c6df58ad0..ee2398416 100644
--- a/modules/nf-core/gffread/environment.yml
+++ b/modules/nf-core/gffread/environment.yml
@@ -1,7 +1,5 @@
-name: gffread
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::gffread=0.12.7
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index ca39fb67e..f1cd99b07 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -1,7 +1,5 @@
-name: multiqc
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::multiqc=1.21
+  - bioconda::multiqc=1.24.1
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 47ac352f9..ceaec139e 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,14 +3,16 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.21--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.24.1--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.24.1--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
     path(multiqc_config)
     path(extra_multiqc_config)
     path(multiqc_logo)
+    path(replace_names)
+    path(sample_names)
 
     output:
     path "*multiqc_report.html", emit: report
@@ -23,16 +25,22 @@ process MULTIQC {
 
     script:
     def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : ''
     def config = multiqc_config ? "--config $multiqc_config" : ''
     def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
-    def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : ''
+    def logo = multiqc_logo ? "--cl-config 'custom_logo: \"${multiqc_logo}\"'" : ''
+    def replace = replace_names ? "--replace-names ${replace_names}" : ''
+    def samples = sample_names ? "--sample-names ${sample_names}" : ''
     """
     multiqc \\
         --force \\
         $args \\
         $config \\
+        $prefix \\
         $extra_config \\
         $logo \\
+        $replace \\
+        $samples \\
         .
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index 45a9bc35e..382c08cbc 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -29,6 +29,19 @@ input:
       type: file
       description: Optional logo file for MultiQC
       pattern: "*.{png}"
+  - replace_names:
+      type: file
+      description: |
+        Optional two-column sample renaming file. First column a set of
+        patterns, second column a set of corresponding replacements. Passed via
+        MultiQC's `--replace-names` option.
+      pattern: "*.{tsv}"
+  - sample_names:
+      type: file
+      description: |
+        Optional TSV file with headers, passed to the MultiQC --sample_names
+        argument.
+      pattern: "*.{tsv}"
 output:
   - report:
       type: file
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
index f1c4242ef..33316a7dd 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -8,6 +8,8 @@ nextflow_process {
     tag "modules_nfcore"
     tag "multiqc"
 
+    config "./nextflow.config"
+
     test("sarscov2 single-end [fastqc]") {
 
         when {
@@ -17,6 +19,8 @@ nextflow_process {
                 input[1] = []
                 input[2] = []
                 input[3] = []
+                input[4] = []
+                input[5] = []
                 """
             }
         }
@@ -41,6 +45,8 @@ nextflow_process {
                 input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
                 input[2] = []
                 input[3] = []
+                input[4] = []
+                input[5] = []
                 """
             }
         }
@@ -66,6 +72,8 @@ nextflow_process {
                 input[1] = []
                 input[2] = []
                 input[3] = []
+                input[4] = []
+                input[5] = []
                 """
             }
         }
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index bfebd8029..83fa080c8 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+                "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-29T08:48:55.657331"
+        "timestamp": "2024-07-10T12:41:34.562023"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+                "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-29T08:49:49.071937"
+        "timestamp": "2024-07-10T11:27:11.933869532"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+                "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-29T08:49:25.457567"
+        "timestamp": "2024-07-10T11:26:56.709849369"
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/multiqc/tests/nextflow.config b/modules/nf-core/multiqc/tests/nextflow.config
new file mode 100644
index 000000000..c537a6a3e
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'MULTIQC' {
+        ext.prefix = null
+    }
+}
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
index 73ba7dc6b..ff4a85edb 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
+++ b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
@@ -1,7 +1,5 @@
-name: picard_collectinsertsizemetrics
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::picard=3.1.1
+  - bioconda::picard=3.2.0
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/main.nf b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
index 06c4bc172..1a49a7f0a 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/main.nf
+++ b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTINSERTSIZEMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' :
-        'biocontainers/picard:3.1.1--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.2.0--hdfd78af_0' :
+        'biocontainers/picard:3.2.0--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam)
@@ -47,6 +47,10 @@ process PICARD_COLLECTINSERTSIZEMETRICS {
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def avail_mem = 3072
+    if (!task.memory) {
+        log.info '[Picard CollectInsertSizeMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
+    }
     """
     touch ${prefix}.pdf
     touch ${prefix}.txt
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test b/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test
new file mode 100644
index 000000000..4cf7a3323
--- /dev/null
+++ b/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test
@@ -0,0 +1,62 @@
+
+nextflow_process {
+
+    name "Test Process PICARD_COLLECTINSERTSIZEMETRICS"
+    script "../main.nf"
+    process "PICARD_COLLECTINSERTSIZEMETRICS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "picard"
+    tag "picard/collectinsertsizemetrics"
+
+    test("test-picard-collectinsertsizemetrics") {
+
+        when {
+            process {
+                """
+                input[0] = [
+				    [ id:'test', single_end:false ], // meta map
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+				]
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+					file(process.out.metrics[0][1]).readLines()[5..8],
+					file(process.out.histogram[0][1]).name,
+					process.out.versions
+					).match()
+				}
+            )
+        }
+    }
+
+    test("test-picard-collectinsertsizemetrics-stub") {
+        options '-stub'
+        when {
+            process {
+                """
+                input[0] = [
+				    [ id:'test', single_end:false ], // meta map
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+				]
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap b/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap
new file mode 100644
index 000000000..bcb5b4557
--- /dev/null
+++ b/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap
@@ -0,0 +1,74 @@
+{
+    "test-picard-collectinsertsizemetrics": {
+        "content": [
+            [
+                "## METRICS CLASS\tpicard.analysis.InsertSizeMetrics",
+                "MEDIAN_INSERT_SIZE\tMODE_INSERT_SIZE\tMEDIAN_ABSOLUTE_DEVIATION\tMIN_INSERT_SIZE\tMAX_INSERT_SIZE\tMEAN_INSERT_SIZE\tSTANDARD_DEVIATION\tREAD_PAIRS\tPAIR_ORIENTATION\tWIDTH_OF_10_PERCENT\tWIDTH_OF_20_PERCENT\tWIDTH_OF_30_PERCENT\tWIDTH_OF_40_PERCENT\tWIDTH_OF_50_PERCENT\tWIDTH_OF_60_PERCENT\tWIDTH_OF_70_PERCENT\tWIDTH_OF_80_PERCENT\tWIDTH_OF_90_PERCENT\tWIDTH_OF_95_PERCENT\tWIDTH_OF_99_PERCENT\tSAMPLE\tLIBRARY\tREAD_GROUP",
+                "209\t159\t46\t77\t364\t207.659794\t66.769018\t97\tFR\t25\t49\t59\t77\t93\t123\t145\t183\t223\t255\t311\t\t\t",
+                ""
+            ],
+            "test.pdf",
+            [
+                "versions.yml:md5,4423bfe2194f61d919c382d2225b0835"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-30T12:23:29.392944"
+    },
+    "test-picard-collectinsertsizemetrics-stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,4423bfe2194f61d919c382d2225b0835"
+                ],
+                "histogram": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "metrics": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,4423bfe2194f61d919c382d2225b0835"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-30T12:20:03.841884"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
index 542b2e823..ff4a85edb 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
+++ b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
@@ -1,7 +1,5 @@
-name: picard_collectrnaseqmetrics
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::picard=3.1.1
+  - bioconda::picard=3.2.0
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/main.nf b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
index f833a105c..0c8f2cfa2 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/main.nf
+++ b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTRNASEQMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' :
-        'biocontainers/picard:3.1.1--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.2.0--hdfd78af_0' :
+        'biocontainers/picard:3.2.0--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/picard/collectwgsmetrics/environment.yml b/modules/nf-core/picard/collectwgsmetrics/environment.yml
index 6a0b9258e..58d525818 100644
--- a/modules/nf-core/picard/collectwgsmetrics/environment.yml
+++ b/modules/nf-core/picard/collectwgsmetrics/environment.yml
@@ -1,8 +1,6 @@
-name: picard_collectwgsmetrics
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::picard=3.1.1
+  - bioconda::picard=3.2.0
   - r::r-base
diff --git a/modules/nf-core/picard/collectwgsmetrics/main.nf b/modules/nf-core/picard/collectwgsmetrics/main.nf
index 35f4129cf..6002a7ca9 100644
--- a/modules/nf-core/picard/collectwgsmetrics/main.nf
+++ b/modules/nf-core/picard/collectwgsmetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTWGSMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' :
-        'biocontainers/picard:3.1.1--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.2.0--hdfd78af_0' :
+        'biocontainers/picard:3.2.0--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test b/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test
new file mode 100644
index 000000000..a3984566d
--- /dev/null
+++ b/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test
@@ -0,0 +1,83 @@
+
+nextflow_process {
+
+    name "Test Process PICARD_COLLECTWGSMETRICS"
+    script "../main.nf"
+    process "PICARD_COLLECTWGSMETRICS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "picard"
+    tag "picard/collectwgsmetrics"
+
+    test("test-picard-collectwgsmetrics") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true),
+				]
+				input[1] = [
+				    [id:'genome'],
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+				]
+				input[2] = [
+				    [id:'genome'],
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)
+				]
+				input[3] = []
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.metrics[0][1]).text.contains('coverage	high_quality_coverage_count'),
+                    process.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("test-picard-collectwgsmetrics-with-interval") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+				    []
+				]
+				input[1] = [
+				    [id:'genome'],
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+				]
+				input[2] = [
+				    [id:'genome'],
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)
+				]
+				input[3] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/picard/baits.interval_list', checkIfExists: true)
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.metrics[0][1]).text.contains('coverage	high_quality_coverage_count'),
+                    process.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap b/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap
new file mode 100644
index 000000000..f188382b0
--- /dev/null
+++ b/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap
@@ -0,0 +1,28 @@
+{
+    "test-picard-collectwgsmetrics-with-interval": {
+        "content": [
+            true,
+            [
+                "versions.yml:md5,06b5898fb06823b736c90e1dcebe75fe"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-23T11:02:46.301176"
+    },
+    "test-picard-collectwgsmetrics": {
+        "content": [
+            true,
+            [
+                "versions.yml:md5,06b5898fb06823b736c90e1dcebe75fe"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-23T11:02:25.132069"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/rrnatranscripts/environment.yml b/modules/nf-core/rrnatranscripts/environment.yml
index e812273bf..6f09494b4 100644
--- a/modules/nf-core/rrnatranscripts/environment.yml
+++ b/modules/nf-core/rrnatranscripts/environment.yml
@@ -1,9 +1,7 @@
 ---
 # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-name: "rrnatranscripts"
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - python=3.12.2
diff --git a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
index 2e320e7bd..ea3ce0f49 100644
--- a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
+++ b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
@@ -10,6 +10,7 @@
 logger = logging.getLogger(__name__)
 logger.setLevel(logging.INFO)
 
+
 def get_rrna_intervals(gtf: str, rrna_transcripts: str):
     """
     Get lines containing ``#`` or ``gene_type rRNA`` or ```` or ``gene_type rRNA_pseudogene`` or ``gene_type MT_rRNA``
@@ -67,19 +68,16 @@ def format_yaml_like(data: dict, indent: int = 0) -> str:
 
 
 if __name__ == "__main__":
-    if '${task.ext.prefix}' != "null":
+    if "${task.ext.prefix}" != "null":
         prefix = "${task.ext.prefix}."
     else:
         prefix = "${task.ext.gtf}."
 
-    if not get_rrna_intervals('$gtf', f"{prefix}_rrna_intervals.gtf"):
+    if not get_rrna_intervals("$gtf", f"{prefix}_rrna_intervals.gtf"):
         logging.error("Failed to extract rrna transcipts.")
 
-
-
     # Write the versions
     versions_this_module = {}
     versions_this_module["${task.process}"] = {"python": platform.python_version()}
     with open("versions.yml", "w") as f:
         f.write(format_yaml_like(versions_this_module))
-
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test b/modules/nf-core/rrnatranscripts/tests/main.nf.test
index 1c29522ad..db0d55652 100644
--- a/modules/nf-core/rrnatranscripts/tests/main.nf.test
+++ b/modules/nf-core/rrnatranscripts/tests/main.nf.test
@@ -14,7 +14,7 @@ nextflow_process {
             process {
                 """
                 input[0] = [
-                            file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
                 ]                """
             }
         }
@@ -36,7 +36,7 @@ nextflow_process {
             process {
                 """
                 input[0] = [
-                            file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
                 ]                """
             }
         }
diff --git a/modules/nf-core/salmon/index/environment.yml b/modules/nf-core/salmon/index/environment.yml
index a6607d964..471164faa 100644
--- a/modules/nf-core/salmon/index/environment.yml
+++ b/modules/nf-core/salmon/index/environment.yml
@@ -1,7 +1,5 @@
-name: salmon_index
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::salmon=1.10.1
diff --git a/modules/nf-core/salmon/quant/environment.yml b/modules/nf-core/salmon/quant/environment.yml
index 862667241..471164faa 100644
--- a/modules/nf-core/salmon/quant/environment.yml
+++ b/modules/nf-core/salmon/quant/environment.yml
@@ -1,7 +1,5 @@
-name: salmon_quant
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::salmon=1.10.1
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index e177b42ac..6c528b240 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -16,9 +16,10 @@ process SALMON_QUANT {
     val   lib_type
 
     output:
-    tuple val(meta), path("${prefix}") , emit: results
-    tuple val(meta), path("*info.json"), emit: json_info, optional: true
-    path  "versions.yml"               , emit: versions
+    tuple val(meta), path("${prefix}")              , emit: results
+    tuple val(meta), path("*info.json")             , emit: json_info, optional: true
+    tuple val(meta), path("*lib_format_counts.json"), emit: lib_format_counts, optional: true
+    path  "versions.yml"                            , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -70,6 +71,9 @@ process SALMON_QUANT {
     if [ -f $prefix/aux_info/meta_info.json ]; then
         cp $prefix/aux_info/meta_info.json "${prefix}_meta_info.json"
     fi
+    if [ -f $prefix/lib_format_counts.json ]; then
+        cp $prefix/lib_format_counts.json "${prefix}_lib_format_counts.json"
+    fi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -82,6 +86,7 @@ process SALMON_QUANT {
     """
     mkdir ${prefix}
     touch ${prefix}_meta_info.json
+    touch ${prefix}_lib_format_counts.json
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/salmon/quant/tests/main.nf.test b/modules/nf-core/salmon/quant/tests/main.nf.test
index b387fac2a..2964cc3d0 100644
--- a/modules/nf-core/salmon/quant/tests/main.nf.test
+++ b/modules/nf-core/salmon/quant/tests/main.nf.test
@@ -48,7 +48,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_single_end") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
@@ -80,7 +83,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_single_end_stub") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
@@ -112,7 +118,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_single_end_lib_type_a") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
@@ -144,7 +153,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_single_end_lib_type_a_stub") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
@@ -179,7 +191,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_pair_end") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
@@ -214,7 +229,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_pair_end stub") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
@@ -251,7 +269,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_pair_end_multiple") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
@@ -288,7 +309,10 @@ nextflow_process {
                 { assert process.success },
                 { assert path(process.out.json_info.get(0).get(1)).exists() },
                 { assert path(process.out.results.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("versions_pair_end_multiple_stub") }
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.lib_format_counts
+                ).match() }
             )
         }
 
diff --git a/modules/nf-core/salmon/quant/tests/main.nf.test.snap b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
index a80bc8aa8..547ce2de1 100644
--- a/modules/nf-core/salmon/quant/tests/main.nf.test.snap
+++ b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
@@ -1,125 +1,170 @@
 {
-    "versions_single_end_lib_type_a_stub": {
+    "sarscov2 - single_end": {
         "content": [
             [
                 "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-04-24T14:34:21.647863"
-    },
-    "versions_pair_end_multiple_stub": {
-        "content": [
+            ],
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test_lib_format_counts.json:md5,c7999dfccd32c090d94e5951522eecd4"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-04-24T14:34:48.03415"
+        "timestamp": "2024-06-14T09:51:22.424672"
     },
     "sarscov2 - single_end stub": {
         "content": [
             [
-                
+                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
             ],
             [
-                
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-04-23T09:34:16.444372"
+        "timestamp": "2024-06-14T09:51:32.292277"
     },
-    "versions_single_end": {
+    "sarscov2 - single_end lib type A": {
         "content": [
             [
                 "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test_lib_format_counts.json:md5,c7999dfccd32c090d94e5951522eecd4"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-01T11:51:01.865730832"
+        "timestamp": "2024-06-14T09:51:42.656382"
     },
-    "versions_pair_end": {
+    "sarscov2 - pair_end multiple": {
         "content": [
             [
                 "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-06T17:10:56.121713"
-    },
-    "versions_pair_end_stub": {
-        "content": [
+            ],
             [
-                
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test_lib_format_counts.json:md5,4a2ee0fac91a4a3471872808d8bd3ff8"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-04-23T08:38:01.276656"
+        "timestamp": "2024-06-14T09:52:33.03647"
     },
-    "versions_pair_end stub": {
+    "sarscov2 - pair_end multiple stub": {
         "content": [
             [
                 "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-04-24T14:34:36.570127"
+        "timestamp": "2024-06-14T09:52:43.388379"
     },
-    "versions_single_end_stub": {
+    "sarscov2 - single_end lib type A stub": {
         "content": [
             [
                 "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-04-24T14:28:01.864343"
+        "timestamp": "2024-06-14T09:51:52.612664"
     },
-    "versions_single_end_lib_type_a": {
+    "sarscov2 - pair_end": {
         "content": [
             [
                 "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test_lib_format_counts.json:md5,e9516e73c9fb39145513b2a41a0af95f"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-06T17:09:14.898351"
+        "timestamp": "2024-06-14T09:52:05.577881"
     },
-    "versions_pair_end_multiple": {
+    "sarscov2 - pair_end stub": {
         "content": [
             [
                 "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-06T17:11:07.846643"
+        "timestamp": "2024-06-14T09:52:15.286461"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml
index 9c24eb0ab..b98cbb990 100644
--- a/modules/nf-core/samtools/faidx/environment.yml
+++ b/modules/nf-core/samtools/faidx/environment.yml
@@ -1,10 +1,7 @@
-name: samtools_faidx
-
 channels:
   - conda-forge
   - bioconda
-  - defaults
 
 dependencies:
-  - bioconda::htslib=1.19.1
-  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.20
+  - bioconda::samtools=1.20
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
index cfe7ad95f..bdcdbc954 100644
--- a/modules/nf-core/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_FAIDX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
-        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
+        'biocontainers/samtools:1.20--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
index 3e651ef68..3223b72bc 100644
--- a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
@@ -18,7 +18,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ],
                 "fa": [
                     
@@ -36,7 +36,7 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ]
             }
         ],
@@ -44,7 +44,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-18T16:22:39.412601"
+        "timestamp": "2024-05-28T15:42:14.779784761"
     },
     "test_samtools_faidx_bgzip": {
         "content": [
@@ -71,7 +71,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ],
                 "fa": [
                     
@@ -95,7 +95,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ]
             }
         ],
@@ -103,7 +103,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-18T16:23:22.427966"
+        "timestamp": "2024-05-28T15:42:20.256633877"
     },
     "test_samtools_faidx_fasta": {
         "content": [
@@ -124,7 +124,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ],
                 "fa": [
                     [
@@ -142,7 +142,7 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ]
             }
         ],
@@ -150,7 +150,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-18T16:24:04.107537"
+        "timestamp": "2024-05-28T15:42:25.632577273"
     },
     "test_samtools_faidx_stub_fasta": {
         "content": [
@@ -171,7 +171,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ],
                 "fa": [
                     [
@@ -189,7 +189,7 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ]
             }
         ],
@@ -197,7 +197,7 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-18T16:24:45.868463"
+        "timestamp": "2024-05-28T15:42:31.058424849"
     },
     "test_samtools_faidx_stub_fai": {
         "content": [
@@ -218,7 +218,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ],
                 "fa": [
                     
@@ -236,7 +236,7 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c"
+                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
                 ]
             }
         ],
@@ -244,6 +244,6 @@
             "nf-test": "0.8.4",
             "nextflow": "23.10.1"
         },
-        "timestamp": "2024-03-18T16:25:27.550554"
+        "timestamp": "2024-05-28T15:42:36.479929617"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml
index a5e506498..da2df5e43 100644
--- a/modules/nf-core/samtools/index/environment.yml
+++ b/modules/nf-core/samtools/index/environment.yml
@@ -1,8 +1,6 @@
-name: samtools_index
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::samtools=1.19.2
-  - bioconda::htslib=1.19.1
+  - bioconda::samtools=1.20
+  - bioconda::htslib=1.20
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index dc14f98d6..e002585b9 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_INDEX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
-        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
+        'biocontainers/samtools:1.20--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input)
@@ -35,10 +35,11 @@ process SAMTOOLS_INDEX {
     """
 
     stub:
+    def args = task.ext.args ?: ''
+    def extension = file(input).getExtension() == 'cram' ?
+                    "crai" : args.contains("-c") ?  "csi" : "bai"
     """
-    touch ${input}.bai
-    touch ${input}.crai
-    touch ${input}.csi
+    touch ${input}.${extension}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test
index bb7756d1c..ca34fb5cd 100644
--- a/modules/nf-core/samtools/index/tests/main.nf.test
+++ b/modules/nf-core/samtools/index/tests/main.nf.test
@@ -9,11 +9,7 @@ nextflow_process {
     tag "samtools/index"
 
     test("bai") {
-
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([
@@ -27,18 +23,13 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(process.out.bai).match("bai") },
-                { assert snapshot(process.out.versions).match("bai_versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
     test("crai") {
-
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([
@@ -52,20 +43,83 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(process.out.crai).match("crai") },
-                { assert snapshot(process.out.versions).match("crai_versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
     test("csi") {
-
         config "./csi.nextflow.config"
 
         when {
-            params {
-                outdir   = "$outputDir"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.csi[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("bai - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("crai - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true)
+                ])
+                """
             }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("csi - stub") {
+        options "-stub"
+        config "./csi.nextflow.config"
+
+        when {
             process {
                 """
                 input[0] = Channel.of([
@@ -79,8 +133,7 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert path(process.out.csi.get(0).get(1)).exists() },
-                { assert snapshot(process.out.versions).match("csi_versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap
index 3dc8e7de8..799d199ce 100644
--- a/modules/nf-core/samtools/index/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap
@@ -1,74 +1,250 @@
 {
-    "crai_versions": {
+    "csi - stub": {
         "content": [
-            [
-                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
-            ]
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ],
+                "bai": [
+                    
+                ],
+                "crai": [
+                    
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-13T16:12:00.324667957"
+        "timestamp": "2024-07-22T16:51:53.9057"
     },
-    "csi_versions": {
+    "crai - stub": {
         "content": [
-            [
-                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
-            ]
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ],
+                "bai": [
+                    
+                ],
+                "crai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-13T16:12:07.885103162"
+        "timestamp": "2024-07-22T16:51:45.931558"
     },
-    "crai": {
+    "bai - stub": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
                 ]
-            ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-12T18:41:38.446424"
+        "timestamp": "2024-07-22T16:51:34.807525"
     },
-    "bai": {
+    "csi": {
         "content": [
+            "test.paired_end.sorted.bam.csi",
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
-                ]
+                "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-12T18:40:46.579747"
+        "timestamp": "2024-07-22T16:52:55.688799"
     },
-    "bai_versions": {
+    "crai": {
         "content": [
-            [
-                "versions.yml:md5,cc4370091670b64bba7c7206403ffb3e"
-            ]
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ],
+                "bai": [
+                    
+                ],
+                "crai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T16:51:17.609533"
+    },
+    "bai": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "csi": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-13T16:11:51.641425452"
+        "timestamp": "2024-07-22T16:51:04.16585"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml
index 4d898e486..da2df5e43 100644
--- a/modules/nf-core/samtools/sort/environment.yml
+++ b/modules/nf-core/samtools/sort/environment.yml
@@ -1,8 +1,6 @@
-name: samtools_sort
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::samtools=1.19.2
-  - bioconda::htslib=1.19.1
+  - bioconda::samtools=1.20
+  - bioconda::htslib=1.20
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index fc374f980..8e019099c 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_SORT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
-        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
+        'biocontainers/samtools:1.20--h50ea8bc_0' }"
 
     input:
     tuple val(meta) , path(bam)
@@ -50,10 +50,20 @@ process SAMTOOLS_SORT {
     """
 
     stub:
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def extension = args.contains("--output-fmt sam") ? "sam" :
+                    args.contains("--output-fmt cram") ? "cram" :
+                    "bam"
     """
-    touch ${prefix}.bam
-    touch ${prefix}.bam.csi
+    touch ${prefix}.${extension}
+    if [ "${extension}" == "bam" ];
+    then
+        touch ${prefix}.${extension}.csi
+    elif [ "${extension}" == "cram" ];
+    then
+        touch ${prefix}.${extension}.crai
+    fi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test
index 8360e2b1f..c2ea9c72a 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test
@@ -30,13 +30,49 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                        process.out.versions
+                ).match()}
             )
         }
     }
 
     test("cram") {
 
+        config "./nextflow_cram.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out.cram.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.crai.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                    process.out.versions
+                ).match()}
+            )
+        }
+    }
+
+    test("bam - stub") {
+
+        options "-stub"
         config "./nextflow.config"
 
         when {
@@ -62,24 +98,21 @@ nextflow_process {
         }
     }
 
-    test("bam_stub") {
+    test("cram - stub") {
 
-        config "./nextflow.config"
         options "-stub"
+        config "./nextflow_cram.config"
 
         when {
-            params {
-                outdir   = "$outputDir"
-            }
             process {
                 """
                 input[0] = Channel.of([
                     [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true)
                 ])
                 input[1] = Channel.of([
                     [ id:'fasta' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
                 ])
                 """
             }
@@ -88,8 +121,7 @@ nextflow_process {
         then {
             assertAll (
                 { assert process.success },
-                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") },
-                { assert snapshot(process.out.versions).match("bam_stub_versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
index 384776564..da38d5d15 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -1,5 +1,35 @@
 {
     "cram": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.cram"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.cram.crai"
+                ]
+            ],
+            [
+                "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T17:19:37.196205"
+    },
+    "bam - stub": {
         "content": [
             {
                 "0": [
@@ -8,7 +38,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
+                        "test.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "1": [
@@ -23,11 +53,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                        "test.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "4": [
-                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
                 ],
                 "bam": [
                     [
@@ -35,7 +65,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
+                        "test.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "crai": [
@@ -50,105 +80,113 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                        "test.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-03-04T15:08:00.830294"
-    },
-    "bam_stub_bam": {
-        "content": [
-            "test.sorted.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-12T19:21:04.364044"
+        "timestamp": "2024-07-22T15:54:46.580756"
     },
-    "bam_stub_versions": {
-        "content": [
-            [
-                "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
-        },
-        "timestamp": "2024-02-13T16:15:00.20800281"
-    },
-    "bam": {
+    "cram - stub": {
         "content": [
             {
                 "0": [
+                    
+                ],
+                "1": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
+                        "test.sorted.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
-                ],
-                "1": [
-                    
                 ],
                 "2": [
-                    
-                ],
-                "3": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                        "test.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
+                ],
+                "3": [
+                    
                 ],
                 "4": [
-                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
                 ],
                 "bam": [
+                    
+                ],
+                "crai": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,bc0b7c25da26384a006ed84cc9e4da23"
+                        "test.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
-                ],
-                "crai": [
-                    
                 ],
                 "cram": [
-                    
-                ],
-                "csi": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam.csi:md5,8d4e836c2fed6c0bf874d5e8cdba5831"
+                        "test.sorted.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
+                ],
+                "csi": [
+                    
                 ],
                 "versions": [
-                    "versions.yml:md5,e6d43fefc9a8bff91c2ce6e3a1716eca"
+                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T15:57:30.505698"
+    },
+    "bam": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,21c992d59615936b99f2ad008aa54400"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.csi"
+                ]
+            ],
+            [
+                "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-03-04T15:07:48.773803"
+        "timestamp": "2024-07-22T15:54:25.872954"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/tests/nextflow_cram.config b/modules/nf-core/samtools/sort/tests/nextflow_cram.config
new file mode 100644
index 000000000..3a8c0188b
--- /dev/null
+++ b/modules/nf-core/samtools/sort/tests/nextflow_cram.config
@@ -0,0 +1,8 @@
+process {
+
+    withName: SAMTOOLS_SORT {
+        ext.prefix  = { "${meta.id}.sorted" }
+        ext.args    = "--write-index --output-fmt cram"
+    }
+
+}
diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml
index b0676f338..da2df5e43 100644
--- a/modules/nf-core/samtools/view/environment.yml
+++ b/modules/nf-core/samtools/view/environment.yml
@@ -1,8 +1,6 @@
-name: samtools_view
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::samtools=1.19.2
-  - bioconda::htslib=1.19.1
+  - bioconda::samtools=1.20
+  - bioconda::htslib=1.20
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
index 5a8989d69..dc611448c 100644
--- a/modules/nf-core/samtools/view/main.nf
+++ b/modules/nf-core/samtools/view/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_VIEW {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' :
-        'biocontainers/samtools:1.19.2--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
+        'biocontainers/samtools:1.20--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input), path(index)
@@ -13,13 +13,15 @@ process SAMTOOLS_VIEW {
     path qname
 
     output:
-    tuple val(meta), path("*.bam"),  emit: bam,     optional: true
-    tuple val(meta), path("*.cram"), emit: cram,    optional: true
-    tuple val(meta), path("*.sam"),  emit: sam,     optional: true
-    tuple val(meta), path("*.bai"),  emit: bai,     optional: true
-    tuple val(meta), path("*.csi"),  emit: csi,     optional: true
-    tuple val(meta), path("*.crai"), emit: crai,    optional: true
-    path  "versions.yml",            emit: versions
+    tuple val(meta), path("${prefix}.bam"),                                    emit: bam,              optional: true
+    tuple val(meta), path("${prefix}.cram"),                                   emit: cram,             optional: true
+    tuple val(meta), path("${prefix}.sam"),                                    emit: sam,              optional: true
+    tuple val(meta), path("${prefix}.${file_type}.bai"),                       emit: bai,              optional: true
+    tuple val(meta), path("${prefix}.${file_type}.csi"),                       emit: csi,              optional: true
+    tuple val(meta), path("${prefix}.${file_type}.crai"),                      emit: crai,             optional: true
+    tuple val(meta), path("${prefix}.unselected.${file_type}"),                emit: unselected,       optional: true
+    tuple val(meta), path("${prefix}.unselected.${file_type}.{bai,csi,crsi}"), emit: unselected_index, optional: true
+    path  "versions.yml",                                                      emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,13 +29,13 @@ process SAMTOOLS_VIEW {
     script:
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     def reference = fasta ? "--reference ${fasta}" : ""
-    def readnames = qname ? "--qname-file ${qname}": ""
-    def file_type = args.contains("--output-fmt sam") ? "sam" :
-                    args.contains("--output-fmt bam") ? "bam" :
-                    args.contains("--output-fmt cram") ? "cram" :
-                    input.getExtension()
+    file_type = args.contains("--output-fmt sam") ? "sam" :
+                args.contains("--output-fmt bam") ? "bam" :
+                args.contains("--output-fmt cram") ? "cram" :
+                input.getExtension()
+    readnames = qname ? "--qname-file ${qname} --output-unselected ${prefix}.unselected.${file_type}": ""
     if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
     """
     samtools \\
@@ -54,14 +56,14 @@ process SAMTOOLS_VIEW {
 
     stub:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def file_type = args.contains("--output-fmt sam") ? "sam" :
-                    args.contains("--output-fmt bam") ? "bam" :
-                    args.contains("--output-fmt cram") ? "cram" :
-                    input.getExtension()
+    prefix = task.ext.prefix ?: "${meta.id}"
+    file_type = args.contains("--output-fmt sam") ? "sam" :
+                args.contains("--output-fmt bam") ? "bam" :
+                args.contains("--output-fmt cram") ? "cram" :
+                input.getExtension()
     if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
 
-    def index = args.contains("--write-index") ? "touch ${prefix}.csi" : ""
+    def index = args.contains("--write-index") ? "touch ${prefix}.${file_type}.csi" : ""
 
     """
     touch ${prefix}.${file_type}
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
index 3dadafae7..27be60d08 100644
--- a/modules/nf-core/samtools/view/meta.yml
+++ b/modules/nf-core/samtools/view/meta.yml
@@ -73,6 +73,15 @@ output:
       type: file
       description: optional CRAM file index
       pattern: "*.{crai}"
+  # unselected and unselected_index are created when passing a qname
+  - unselected:
+      type: file
+      description: optional file with unselected alignments
+      pattern: "*.unselected.{bam,cram,sam}"
+  - unselected_index:
+      type: file
+      description: index for the "unselected" file
+      pattern: "*.unselected.{bai,csi,crai}"
   - versions:
       type: file
       description: File containing software versions
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test b/modules/nf-core/samtools/view/tests/main.nf.test
index 45a0defba..37b81a916 100644
--- a/modules/nf-core/samtools/view/tests/main.nf.test
+++ b/modules/nf-core/samtools/view/tests/main.nf.test
@@ -172,6 +172,8 @@ nextflow_process {
                 { assert snapshot(process.out.crai).match("cram_to_bam_index_qname_crai") },
                 { assert snapshot(process.out.cram).match("cram_to_bam_index_qname_cram") },
                 { assert snapshot(process.out.sam).match("cram_to_bam_index_qname_sam") },
+                { assert snapshot(file(process.out.unselected[0][1]).name).match("cram_to_bam_index_qname_unselected") },
+                { assert snapshot(file(process.out.unselected_index[0][1]).name).match("cram_to_bam_index_qname_unselected_csi") },
                 { assert snapshot(process.out.versions).match("cram_to_bam_index_qname_versions") }
             )
         }
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap
index f55943a7a..6bcce9fea 100644
--- a/modules/nf-core/samtools/view/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/view/tests/main.nf.test.snap
@@ -56,14 +56,14 @@
     "bam_stub_versions": {
         "content": [
             [
-                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-13T16:13:09.713353823"
+        "timestamp": "2024-05-28T15:43:20.390692583"
     },
     "cram_to_bam_index_cram": {
         "content": [
@@ -208,14 +208,14 @@
     "cram_to_bam_index_qname_versions": {
         "content": [
             [
-                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-13T16:13:03.935041046"
+        "timestamp": "2024-05-28T15:43:15.007493874"
     },
     "cram_to_bam_bam": {
         "content": [
@@ -240,14 +240,14 @@
     "cram_to_bam_index_versions": {
         "content": [
             [
-                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-13T16:12:55.910685496"
+        "timestamp": "2024-05-28T15:43:09.472376824"
     },
     "cram_to_bam_bai": {
         "content": [
@@ -264,14 +264,14 @@
     "cram_to_bam_versions": {
         "content": [
             [
-                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-13T16:12:47.715221169"
+        "timestamp": "2024-05-28T15:43:04.080050906"
     },
     "cram_bam": {
         "content": [
@@ -355,17 +355,37 @@
         },
         "timestamp": "2024-02-12T19:38:23.322874"
     },
+    "cram_to_bam_index_qname_unselected": {
+        "content": [
+            "test.unselected.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.322874"
+    },
+    "cram_to_bam_index_qname_unselected_csi": {
+        "content": [
+            "test.unselected.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.328458"
+    },
     "bam_versions": {
         "content": [
             [
-                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-13T16:12:31.692607421"
+        "timestamp": "2024-05-28T15:42:52.978954857"
     },
     "cram_to_bam_index_qname_cram": {
         "content": [
@@ -430,14 +450,14 @@
     "cram_versions": {
         "content": [
             [
-                "versions.yml:md5,4ea32c57d546102a1b32d9693ada7cf1"
+                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "24.01.0"
+            "nextflow": "23.10.1"
         },
-        "timestamp": "2024-02-13T16:12:39.913411036"
+        "timestamp": "2024-05-28T15:42:58.400776109"
     },
     "bam_sam": {
         "content": [
@@ -477,7 +497,7 @@
     },
     "bam_stub_csi": {
         "content": [
-            "test.csi"
+            "test.bam.csi"
         ],
         "meta": {
             "nf-test": "0.8.4",
diff --git a/modules/nf-core/star/align/environment.yml b/modules/nf-core/star/align/environment.yml
index 8bd58cff5..e3d1c714d 100644
--- a/modules/nf-core/star/align/environment.yml
+++ b/modules/nf-core/star/align/environment.yml
@@ -1,8 +1,6 @@
-name: star_align
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::star=2.7.10a
   - bioconda::samtools=1.18
diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index 8e9c48b1c..ae67e0040 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -81,6 +81,8 @@ process STAR_ALIGN {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
+    echo "" | gzip > ${prefix}.unmapped_1.fastq.gz
+    echo "" | gzip > ${prefix}.unmapped_2.fastq.gz
     touch ${prefix}Xd.out.bam
     touch ${prefix}.Log.final.out
     touch ${prefix}.Log.out
@@ -89,8 +91,6 @@ process STAR_ALIGN {
     touch ${prefix}.toTranscriptome.out.bam
     touch ${prefix}.Aligned.unsort.out.bam
     touch ${prefix}.Aligned.sortedByCoord.out.bam
-    touch ${prefix}.unmapped_1.fastq.gz
-    touch ${prefix}.unmapped_2.fastq.gz
     touch ${prefix}.tab
     touch ${prefix}.SJ.out.tab
     touch ${prefix}.ReadsPerGene.out.tab
diff --git a/modules/nf-core/star/align/tests/main.nf.test b/modules/nf-core/star/align/tests/main.nf.test
index 6ecd77863..2d9f72ddc 100644
--- a/modules/nf-core/star/align/tests/main.nf.test
+++ b/modules/nf-core/star/align/tests/main.nf.test
@@ -9,27 +9,367 @@ nextflow_process {
     tag "star/align"
     tag "star/genomegenerate"
 
-    setup {
-        run("STAR_GENOMEGENERATE") {
-            script "../../../star/genomegenerate/main.nf"
+    test("homo_sapiens - single_end") {
+        config "./nextflow.config"
+
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
+        when {
             process {
                 """
                 input[0] = Channel.of([
-                    [ id:'test_fasta' ],
-                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true) ]
                 ])
-                input[1] = Channel.of([
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
                     [ id:'test_gtf' ],
                     [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
                 ])
+                input[3] = false
+                input[4] = 'illumina'
+                input[5] = false
                 """
             }
         }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.log_final[0][1]).name,
+                    file(process.out.log_out[0][1]).name,
+                    file(process.out.log_progress[0][1]).name,
+                    process.out.bam,
+                    process.out.bam_sorted,
+                    process.out.bam_transcript,
+                    process.out.bam_unsorted,
+                    process.out.bedgraph,
+                    process.out.fastq,
+                    process.out.junction,
+                    process.out.read_per_gene_tab,
+                    process.out.sam,
+                    process.out.spl_junc_tab,
+                    process.out.tab,
+                    process.out.wig,
+                    process.out.versions
+                ).match() }
+            )
+        }
     }
 
-    test("homo_sapiens - single_end") {
+    test("homo_sapiens - paired_end") {
+        config "./nextflow.config"
+
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = false
+                input[4] = 'illumina'
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.log_final[0][1]).name,
+                    file(process.out.log_out[0][1]).name,
+                    file(process.out.log_progress[0][1]).name,
+                    process.out.bam,
+                    process.out.bam_sorted,
+                    process.out.bam_transcript,
+                    process.out.bam_unsorted,
+                    process.out.bedgraph,
+                    process.out.fastq,
+                    process.out.junction,
+                    process.out.read_per_gene_tab,
+                    process.out.sam,
+                    process.out.spl_junc_tab,
+                    process.out.tab,
+                    process.out.wig,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - paired_end - arriba") {
+        config "./nextflow.arriba.config"
+
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = false
+                input[4] = 'illumina'
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.log_final[0][1]).name,
+                    file(process.out.log_out[0][1]).name,
+                    file(process.out.log_progress[0][1]).name,
+                    process.out.bam,
+                    process.out.bam_sorted,
+                    process.out.bam_transcript,
+                    process.out.bam_unsorted,
+                    process.out.bedgraph,
+                    process.out.fastq,
+                    process.out.junction,
+                    process.out.read_per_gene_tab,
+                    process.out.sam,
+                    process.out.spl_junc_tab,
+                    process.out.tab,
+                    process.out.wig,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - paired_end - starfusion") {
+        config "./nextflow.starfusion.config"
+
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = false
+                input[4] = 'illumina'
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.log_final[0][1]).name,
+                    file(process.out.log_out[0][1]).name,
+                    file(process.out.log_progress[0][1]).name,
+                    process.out.bam,
+                    process.out.bam_sorted,
+                    process.out.bam_transcript,
+                    process.out.bam_unsorted,
+                    process.out.bedgraph,
+                    process.out.fastq,
+                    process.out.junction,
+                    process.out.read_per_gene_tab,
+                    process.out.sam,
+                    process.out.spl_junc_tab,
+                    process.out.tab,
+                    process.out.wig,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - paired_end - multiple") {
         config "./nextflow.config"
 
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = false
+                input[4] = 'illumina'
+                input[5] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.log_final[0][1]).name,
+                    file(process.out.log_out[0][1]).name,
+                    file(process.out.log_progress[0][1]).name,
+                    process.out.bam,
+                    process.out.bam_sorted,
+                    process.out.bam_transcript,
+                    process.out.bam_unsorted,
+                    process.out.bedgraph,
+                    process.out.fastq,
+                    process.out.junction,
+                    process.out.read_per_gene_tab,
+                    process.out.sam,
+                    process.out.spl_junc_tab,
+                    process.out.tab,
+                    process.out.wig,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("homo_sapiens - single_end - stub") {
+        options "-stub"
+        config "./nextflow.config"
+
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
         when {
             process {
                 """
@@ -52,29 +392,33 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.log_final[0][1]).name).match("homo_sapiens - single_end - log_final") },
-                { assert snapshot(file(process.out.log_out[0][1]).name).match("homo_sapiens - single_end - log_out") },
-                { assert snapshot(process.out.bam).match("homo_sapiens - single_end - bam") },
-                { assert snapshot(process.out.bam_sorted).match("homo_sapiens - single_end - bam_sorted") },
-                { assert snapshot(process.out.bam_transcript).match("homo_sapiens - single_end - bam_transcript") },
-                { assert snapshot(process.out.bam_unsorted).match("homo_sapiens - single_end - bam_unsorted") },
-                { assert snapshot(process.out.bedgraph).match("homo_sapiens - single_end - bedgraph") },
-                { assert snapshot(process.out.fastq).match("homo_sapiens - single_end - fastq") },
-                { assert snapshot(process.out.junction).match("homo_sapiens - single_end - junction") },
-                { assert snapshot(process.out.log_progress).match("homo_sapiens - single_end - log_progress") },
-                { assert snapshot(process.out.read_per_gene_tab).match("homo_sapiens - single_end - read_per_gene_tab") },
-                { assert snapshot(process.out.sam).match("homo_sapiens - single_end - sam") },
-                { assert snapshot(process.out.spl_junc_tab).match("homo_sapiens - single_end - spl_junc_tab") },
-                { assert snapshot(process.out.tab).match("homo_sapiens - single_end - tab") },
-                { assert snapshot(process.out.wig).match("homo_sapiens - single_end - wig") },
-                { assert snapshot(process.out.versions).match("homo_sapiens - single_end - versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("homo_sapiens - paired_end") {
+    test("homo_sapiens - paired_end - stub") {
+        options "-stub"
         config "./nextflow.config"
 
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
         when {
             process {
                 """
@@ -100,29 +444,33 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.log_final[0][1]).name).match("homo_sapiens - paired_end - log_final") },
-                { assert snapshot(file(process.out.log_out[0][1]).name).match("homo_sapiens - paired_end - log_out") },
-                { assert snapshot(process.out.bam).match("homo_sapiens - paired_end - bam") },
-                { assert snapshot(process.out.bam_sorted).match("homo_sapiens - paired_end - bam_sorted") },
-                { assert snapshot(process.out.bam_transcript).match("homo_sapiens - paired_end - bam_transcript") },
-                { assert snapshot(process.out.bam_unsorted).match("homo_sapiens - paired_end - bam_unsorted") },
-                { assert snapshot(process.out.bedgraph).match("homo_sapiens - paired_end - bedgraph") },
-                { assert snapshot(process.out.fastq).match("homo_sapiens - paired_end - fastq") },
-                { assert snapshot(process.out.junction).match("homo_sapiens - paired_end - junction") },
-                { assert snapshot(process.out.log_progress).match("homo_sapiens - paired_end - log_progress") },
-                { assert snapshot(process.out.read_per_gene_tab).match("homo_sapiens - paired_end - read_per_gene_tab") },
-                { assert snapshot(process.out.sam).match("homo_sapiens - paired_end - sam") },
-                { assert snapshot(process.out.spl_junc_tab).match("homo_sapiens - paired_end - spl_junc_tab") },
-                { assert snapshot(process.out.tab).match("homo_sapiens - paired_end - tab") },
-                { assert snapshot(process.out.wig).match("homo_sapiens - paired_end - wig") },
-                { assert snapshot(process.out.versions).match("homo_sapiens - paired_end - versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("homo_sapiens - paired_end - arriba") {
+    test("homo_sapiens - paired_end - arriba - stub") {
+        options "-stub"
         config "./nextflow.arriba.config"
 
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
         when {
             process {
                 """
@@ -148,29 +496,33 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.log_final[0][1]).name).match("homo_sapiens - paired_end - arriba - log_final") },
-                { assert snapshot(file(process.out.log_out[0][1]).name).match("homo_sapiens - paired_end - arriba - log_out") },
-                { assert snapshot(file(process.out.log_progress[0][1]).name).match("homo_sapiens - paired_end - arriba - log_progress") },
-                { assert snapshot(process.out.bam).match("homo_sapiens - paired_end - arriba - bam") },
-                { assert snapshot(process.out.bam_sorted).match("homo_sapiens - paired_end - arriba - bam_sorted") },
-                { assert snapshot(process.out.bam_transcript).match("homo_sapiens - paired_end - arriba - bam_transcript") },
-                { assert snapshot(process.out.bam_unsorted).match("homo_sapiens - paired_end - arriba - bam_unsorted") },
-                { assert snapshot(process.out.bedgraph).match("homo_sapiens - paired_end - arriba - bedgraph") },
-                { assert snapshot(process.out.fastq).match("homo_sapiens - paired_end - arriba - fastq") },
-                { assert snapshot(process.out.junction).match("homo_sapiens - paired_end - arriba - junction") },
-                { assert snapshot(process.out.read_per_gene_tab).match("homo_sapiens - paired_end - arriba - read_per_gene_tab") },
-                { assert snapshot(process.out.sam).match("homo_sapiens - paired_end - arriba - sam") },
-                { assert snapshot(process.out.spl_junc_tab).match("homo_sapiens - paired_end - arriba - spl_junc_tab") },
-                { assert snapshot(process.out.tab).match("homo_sapiens - paired_end - arriba - tab") },
-                { assert snapshot(process.out.wig).match("homo_sapiens - paired_end - arriba - wig") },
-                { assert snapshot(process.out.versions).match("homo_sapiens - paired_end - arriba - versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("homo_sapiens - paired_end - starfusion") {
+    test("homo_sapiens - paired_end - starfusion - stub") {
+        options "-stub"
         config "./nextflow.starfusion.config"
 
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
         when {
             process {
                 """
@@ -196,29 +548,33 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.log_final[0][1]).name).match("homo_sapiens - paired_end - starfusion - log_final") },
-                { assert snapshot(file(process.out.log_out[0][1]).name).match("homo_sapiens - paired_end - starfusion - log_out") },
-                { assert snapshot(file(process.out.log_progress[0][1]).name).match("homo_sapiens - paired_end - starfusion - log_progress") },
-                { assert snapshot(process.out.bam).match("homo_sapiens - paired_end - starfusion - bam") },
-                { assert snapshot(process.out.bam_sorted).match("homo_sapiens - paired_end - starfusion - bam_sorted") },
-                { assert snapshot(process.out.bam_transcript).match("homo_sapiens - paired_end - starfusion - bam_transcript") },
-                { assert snapshot(process.out.bam_unsorted).match("homo_sapiens - paired_end - starfusion - bam_unsorted") },
-                { assert snapshot(process.out.bedgraph).match("homo_sapiens - paired_end - starfusion - bedgraph") },
-                { assert snapshot(process.out.fastq).match("homo_sapiens - paired_end - starfusion - fastq") },
-                { assert snapshot(process.out.junction).match("homo_sapiens - paired_end - starfusion - junction") },
-                { assert snapshot(process.out.read_per_gene_tab).match("homo_sapiens - paired_end - starfusion - read_per_gene_tab") },
-                { assert snapshot(process.out.sam).match("homo_sapiens - paired_end - starfusion - sam") },
-                { assert snapshot(process.out.spl_junc_tab).match("homo_sapiens - paired_end - starfusion - spl_junc_tab") },
-                { assert snapshot(process.out.tab).match("homo_sapiens - paired_end - starfusion - tab") },
-                { assert snapshot(process.out.wig).match("homo_sapiens - paired_end - starfusion - wig") },
-                { assert snapshot(process.out.versions).match("homo_sapiens - paired_end - starfusion - versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
-    test("homo_sapiens - paired_end - multiple") {
+    test("homo_sapiens - paired_end - multiple - stub") {
+        options "-stub"
         config "./nextflow.config"
 
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../star/genomegenerate/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[1] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    """
+                }
+            }
+        }
+
         when {
             process {
                 """
@@ -246,22 +602,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.log_final[0][1]).name).match("homo_sapiens - paired_end - multiple - log_final") },
-                { assert snapshot(file(process.out.log_out[0][1]).name).match("homo_sapiens - paired_end - multiple - log_out") },
-                { assert snapshot(file(process.out.log_progress[0][1]).name).match("homo_sapiens - paired_end - multiple - log_progress") },
-                { assert snapshot(process.out.bam).match("homo_sapiens - paired_end - multiple - bam") },
-                { assert snapshot(process.out.bam_sorted).match("homo_sapiens - paired_end - multiple - bam_sorted") },
-                { assert snapshot(process.out.bam_transcript).match("homo_sapiens - paired_end - multiple - bam_transcript") },
-                { assert snapshot(process.out.bam_unsorted).match("homo_sapiens - paired_end - multiple - bam_unsorted") },
-                { assert snapshot(process.out.bedgraph).match("homo_sapiens - paired_end - multiple - bedgraph") },
-                { assert snapshot(process.out.fastq).match("homo_sapiens - paired_end - multiple - fastq") },
-                { assert snapshot(process.out.junction).match("homo_sapiens - paired_end - multiple - junction") },
-                { assert snapshot(process.out.read_per_gene_tab).match("homo_sapiens - paired_end - multiple - read_per_gene_tab") },
-                { assert snapshot(process.out.sam).match("homo_sapiens - paired_end - multiple - sam") },
-                { assert snapshot(process.out.spl_junc_tab).match("homo_sapiens - paired_end - multiple - spl_junc_tab") },
-                { assert snapshot(process.out.tab).match("homo_sapiens - paired_end - multiple - tab") },
-                { assert snapshot(process.out.wig).match("homo_sapiens - paired_end - multiple - wig") },
-                { assert snapshot(process.out.versions).match("homo_sapiens - paired_end - multiple - versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/star/align/tests/main.nf.test.snap b/modules/nf-core/star/align/tests/main.nf.test.snap
index 08edb914b..c814eb56c 100644
--- a/modules/nf-core/star/align/tests/main.nf.test.snap
+++ b/modules/nf-core/star/align/tests/main.nf.test.snap
@@ -1,382 +1,1170 @@
 {
-    "homo_sapiens - paired_end - multiple - bam_sorted": {
+    "homo_sapiens - single_end - stub": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Aligned.sortedByCoord.out.bam:md5,ab07c21d63ab0a6c07d171d213c81d5a"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "10": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "11": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "12": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "13": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "14": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "15": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        [
+                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
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             [
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         "content": [
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+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
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+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
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+                        {
+                            "id": "test",
+                            "single_end": false
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+                            "single_end": false
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+                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
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+                            "single_end": false
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+                            "single_end": false
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+                            "single_end": false
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e"
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+                ],
+                "fastq": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
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+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
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+                "log_final": [
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+                            "single_end": false
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+                            "single_end": false
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+                        "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e"
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+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
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+                "tab": [
+                    [
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+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
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+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
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+                "versions": [
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+                            "single_end": false
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+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
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-        "timestamp": "2023-11-23T13:27:56.082408"
+        "meta": {
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+            "nextflow": "24.04.3"
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+        "timestamp": "2024-07-22T15:16:51.360287"
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-    "homo_sapiens - paired_end - starfusion - tab": {
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         "content": [
+            "test.Log.final.out",
+            "test.Log.out",
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             [
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                     {
                         "id": "test",
                         "single_end": false
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-        "timestamp": "2023-12-04T17:59:58.818041322"
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-    "homo_sapiens - single_end - fastq": {
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+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
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+                    "test.Aligned.sortedByCoord.out.bam:md5,ab07c21d63ab0a6c07d171d213c81d5a"
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-        "timestamp": "2023-11-23T13:22:55.175307"
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+            [
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                     {
                         "id": "test",
                         "single_end": false
                     },
-                    "test.SJ.out.tab:md5,844af19ab0fc8cd9a3f75228445aca0d"
+                    [
+                        "test.Signal.Unique.str1.out.bg:md5,d7bf8b70b436ca048a62513e1d0ece3a",
+                        "test.Signal.UniqueMultiple.str1.out.bg:md5,686d58493b9eb445b56ace4d67f76ef6"
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-        "timestamp": "2023-12-04T17:54:12.255481058"
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             [
                 
-            ]
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-        "timestamp": "2023-11-23T13:27:56.155413"
-    },
-    "homo_sapiens - single_end - bam_transcript": {
-        "content": [
+            ],
             [
                 
-            ]
-        ],
-        "timestamp": "2023-11-23T13:22:55.144852"
-    },
-    "homo_sapiens - paired_end - versions": {
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             [
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-        "timestamp": "2023-12-04T17:54:12.343840482"
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-    "homo_sapiens - paired_end - multiple - tab": {
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+            ],
+            [
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             [
                 [
                     {
@@ -385,385 +1173,801 @@
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                     "test.SJ.out.tab:md5,069877e053714e23010fe4e1c003b4a2"
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-        "timestamp": "2023-12-04T18:01:20.291692062"
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                     {
                         "id": "test",
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+                        "single_end": false
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-        "timestamp": "2023-12-04T17:53:26.265642675"
-    },
-    "homo_sapiens - paired_end - arriba - wig": {
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+            ],
             [
                 
+            ],
+            [
+                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
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         ],
-        "timestamp": "2023-11-23T13:25:07.444214"
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+        "timestamp": "2024-07-22T13:13:28.987438"
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+                        },
+                        [
+                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "8": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "9": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "bam_sorted": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "bam_transcript": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam_unsorted": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bedgraph": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastq": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "junction": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log_final": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log_out": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log_progress": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "read_per_gene_tab": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "sam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "spl_junc_tab": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tab": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                ],
+                "wig": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
         ],
-        "timestamp": "2023-11-23T13:27:55.93945"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T15:16:40.64399"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/star/genomegenerate/environment.yml b/modules/nf-core/star/genomegenerate/environment.yml
index 791f255e5..38611dfa2 100644
--- a/modules/nf-core/star/genomegenerate/environment.yml
+++ b/modules/nf-core/star/genomegenerate/environment.yml
@@ -1,8 +1,6 @@
-name: star_genomegenerate
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::samtools=1.18
   - bioconda::htslib=1.18
diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test b/modules/nf-core/star/genomegenerate/tests/main.nf.test
index c17c8ba45..4d619c473 100644
--- a/modules/nf-core/star/genomegenerate/tests/main.nf.test
+++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test
@@ -28,15 +28,15 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_index") },
-                { assert snapshot(process.out.versions).match("fasta_gtf_versions") }
+                { assert snapshot(
+                    file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString(),
+                    process.out.versions)
+                .match() }
             )
         }
     }
 
-    test("fasta_gtf_stub") {
-
-        options '-stub'
+    test("fasta") {
 
         when {
             process {
@@ -45,10 +45,7 @@ nextflow_process {
                     [ id:'test_fasta' ],
                     [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
                 ])
-                input[1] = Channel.of([
-                    [ id:'test_gtf' ],
-                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
-                ])
+                input[1] = Channel.of([ [], [] ])
                 """
             }
         }
@@ -56,13 +53,17 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_stub_index") },
-                { assert snapshot(process.out.versions).match("fasta_gtf_stub_versions") }
+                { assert snapshot(
+                    file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString(),
+                    process.out.versions
+                ).match() }
             )
         }
     }
 
-    test("fasta") {
+    test("fasta_gtf_stub") {
+
+        options '-stub'
 
         when {
             process {
@@ -71,7 +72,10 @@ nextflow_process {
                     [ id:'test_fasta' ],
                     [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
                 ])
-                input[1] = Channel.of([ [], [] ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
                 """
             }
         }
@@ -79,11 +83,9 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_index") },
-                { assert snapshot(process.out.versions).match("fasta_versions") }
+                { assert snapshot(process.out).match() }
             )
         }
-
     }
 
     test("fasta_stub") {
@@ -105,11 +107,8 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_stub_index") },
-                { assert snapshot(process.out.versions).match("fasta_stub_versions") }
+                { assert snapshot(process.out).match() }
             )
         }
-
     }
-
 }
diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
index 5653d6e6c..207f4b4f5 100644
--- a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
+++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
@@ -1,90 +1,148 @@
 {
-    "fasta_gtf_versions": {
+    "fasta_gtf": {
         "content": [
+            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]",
             [
                 "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-01T15:54:31.798555"
+        "timestamp": "2024-07-22T14:55:35.478401"
     },
-    "fasta_stub_versions": {
+    "fasta_gtf_stub": {
         "content": [
-            [
-                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-01T15:55:07.521209"
-    },
-    "fasta_gtf_stub_index": {
-        "content": [
-            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-01T15:54:46.478098"
-    },
-    "fasta_gtf_stub_versions": {
-        "content": [
-            [
-                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
-            ]
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_fasta"
+                        },
+                        [
+                            "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                ],
+                "index": [
+                    [
+                        {
+                            "id": "test_fasta"
+                        },
+                        [
+                            "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-01T15:54:46.491657"
+        "timestamp": "2024-07-22T14:55:57.247585"
     },
-    "fasta_index": {
+    "fasta_stub": {
         "content": [
-            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_fasta"
+                        },
+                        [
+                            "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                ],
+                "index": [
+                    [
+                        {
+                            "id": "test_fasta"
+                        },
+                        [
+                            "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-01T15:54:57.552329"
+        "timestamp": "2024-07-22T14:56:07.01742"
     },
-    "fasta_versions": {
+    "fasta": {
         "content": [
+            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]",
             [
                 "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-01T15:54:57.560541"
-    },
-    "fasta_gtf_index": {
-        "content": [
-            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-01T15:54:31.786814"
-    },
-    "fasta_stub_index": {
-        "content": [
-            "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-02-01T15:55:07.517472"
+        "timestamp": "2024-07-22T14:55:45.48784"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/stringtie/merge/environment.yml b/modules/nf-core/stringtie/merge/environment.yml
index 9914b2025..0556de41a 100644
--- a/modules/nf-core/stringtie/merge/environment.yml
+++ b/modules/nf-core/stringtie/merge/environment.yml
@@ -1,7 +1,5 @@
-name: stringtie_merge
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::stringtie=2.2.1
diff --git a/modules/nf-core/stringtie/merge/tests/main.nf.test b/modules/nf-core/stringtie/merge/tests/main.nf.test
index 358c561a0..bcc648bc7 100644
--- a/modules/nf-core/stringtie/merge/tests/main.nf.test
+++ b/modules/nf-core/stringtie/merge/tests/main.nf.test
@@ -50,7 +50,7 @@ nextflow_process {
             process {
                 """
                 input[0] = STRINGTIE_STRINGTIE.out.transcript_gtf.map { it -> it[1] }
-                input[1] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
+                input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
                 """
             }
         }
diff --git a/modules/nf-core/stringtie/stringtie/environment.yml b/modules/nf-core/stringtie/stringtie/environment.yml
index 7a0eccdb8..0556de41a 100644
--- a/modules/nf-core/stringtie/stringtie/environment.yml
+++ b/modules/nf-core/stringtie/stringtie/environment.yml
@@ -1,7 +1,5 @@
-name: stringtie_stringtie
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::stringtie=2.2.1
diff --git a/modules/nf-core/stringtie/stringtie/tests/main.nf.test b/modules/nf-core/stringtie/stringtie/tests/main.nf.test
index 00efe8f1a..2204e849f 100644
--- a/modules/nf-core/stringtie/stringtie/tests/main.nf.test
+++ b/modules/nf-core/stringtie/stringtie/tests/main.nf.test
@@ -3,6 +3,7 @@ nextflow_process {
     name "Test Process STRINGTIE_STRINGTIE"
     script "../main.nf"
     process "STRINGTIE_STRINGTIE"
+    config "./nextflow.config"
     tag "modules"
     tag "modules_nfcore"
     tag "stringtie"
@@ -10,8 +11,6 @@ nextflow_process {
 
     test("sarscov2 [bam] - forward strandedness") {
 
-        config "./nextflow.config"
-
         when {
             process {
                 """
@@ -27,17 +26,17 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.transcript_gtf).match("fs_transcript_gtf") },
-                { assert snapshot(process.out.abundance).match("fs_abundance") },
-                { assert snapshot(process.out.versions).match("fs_versions") }
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
             )
         }
     }
 
     test("sarscov2 [bam] - forward strandedness + reference annotation") {
 
-        config "./nextflow.config"
-
         when {
             process {
                 """
@@ -53,18 +52,18 @@ nextflow_process {
         then {
             assertAll(
             { assert process.success },
-                { assert snapshot(process.out.transcript_gtf).match("fs_gtf_transcript_gtf") },
-                { assert snapshot(process.out.abundance).match("fs_gtf_abundance") },
-                { assert snapshot(process.out.ballgown).match("fs_gtf_ballgown") },
-                { assert snapshot(process.out.versions).match("fs_gtf_versions") }
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.ballgown,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
             )
         }
     }
 
     test("sarscov2 [bam] - reverse strandedness") {
 
-        config "./nextflow.config"
-
         when {
             process {
                 """
@@ -80,16 +79,117 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.transcript_gtf).match("rs_transcript_gtf") },
-                { assert snapshot(process.out.abundance).match("rs_abundance") },
-                { assert snapshot(process.out.versions).match("rs_versions") }
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
             )
         }
     }
 
     test("sarscov2 [bam] - reverse strandedness + reference annotation") {
 
-        config "./nextflow.config"
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'reverse' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.abundance,
+                    process.out.ballgown,
+                    process.out.transcript_gtf,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - forward strandedness - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'forward' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - forward strandedness + reference annotation - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', strandedness:'forward' ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                ]
+                input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gtf", checkIfExists: true)
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse strandedness - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                            [ id:'test', strandedness:'reverse' ], // meta map
+                            [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true) ]
+                        ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 [bam] - reverse strandedness + reference annotation - stub") {
+
+        options "-stub"
 
         when {
             process {
@@ -106,10 +206,7 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.transcript_gtf).match("rs_gtf_transcript_gtf") },
-                { assert snapshot(process.out.abundance).match("rs_gtf_abundance") },
-                { assert snapshot(process.out.ballgown).match("rs_gtf_ballgown") },
-                { assert snapshot(process.out.versions).match("rs_gtf_versions") }
+                { assert snapshot(process.out).match() }
             )
         }
     }
diff --git a/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap b/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
index bf7516364..124dd4cbe 100644
--- a/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
+++ b/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "fs_abundance": {
+    "sarscov2 [bam] - forward strandedness + reference annotation": {
         "content": [
             [
                 [
@@ -7,49 +7,44 @@
                         "id": "test",
                         "strandedness": "forward"
                     },
-                    "test.gene.abundance.txt:md5,d6f5c8cadb8458f1df0427cf790246e3"
+                    "test.gene.abundance.txt:md5,7d8bce7f2a922e367cedccae7267c22e"
                 ]
-            ]
-        ],
-        "timestamp": "2023-11-23T13:55:41.032044613"
-    },
-    "fs_transcript_gtf": {
-        "content": [
+            ],
             [
                 [
                     {
                         "id": "test",
                         "strandedness": "forward"
                     },
-                    "test.transcripts.gtf:md5,569137af5be452413086b50653a97203"
+                    [
+                        "e2t.ctab:md5,e981c0038295ae54b63cedb1083f1540",
+                        "e_data.ctab:md5,6b4cf69bc03f3f69890f972a0e8b7471",
+                        "i2t.ctab:md5,8a117c8aa4334b4c2d4711932b006fb4",
+                        "i_data.ctab:md5,be3abe09740603213f83d50dcf81427f",
+                        "t_data.ctab:md5,3b66c065da73ae0dd41cc332eff6a818"
+                    ]
                 ]
-            ]
-        ],
-        "timestamp": "2023-11-23T13:55:41.017978904"
-    },
-    "rs_abundance": {
-        "content": [
+            ],
             [
                 [
                     {
                         "id": "test",
-                        "strandedness": "reverse"
+                        "strandedness": "forward"
                     },
-                    "test.gene.abundance.txt:md5,d6f5c8cadb8458f1df0427cf790246e3"
+                    "test.transcripts.gtf:md5,f56cf8aba2c4a5673bc7963ba5f12d04"
                 ]
-            ]
-        ],
-        "timestamp": "2023-11-23T13:56:13.601112933"
-    },
-    "fs_gtf_versions": {
-        "content": [
+            ],
             [
                 "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
             ]
         ],
-        "timestamp": "2023-11-23T13:56:00.523797974"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:33:44.299962"
     },
-    "fs_gtf_transcript_gtf": {
+    "sarscov2 [bam] - forward strandedness": {
         "content": [
             [
                 [
@@ -57,50 +52,395 @@
                         "id": "test",
                         "strandedness": "forward"
                     },
-                    "test.transcripts.gtf:md5,f56cf8aba2c4a5673bc7963ba5f12d04"
+                    "test.gene.abundance.txt:md5,d6f5c8cadb8458f1df0427cf790246e3"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "strandedness": "forward"
+                    },
+                    "test.transcripts.gtf:md5,569137af5be452413086b50653a97203"
                 ]
+            ],
+            [
+                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
             ]
         ],
-        "timestamp": "2023-11-23T13:56:00.475164879"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:33:35.177738"
     },
-    "rs_versions": {
+    "sarscov2 [bam] - forward strandedness - stub": {
         "content": [
-            [
-                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
-            ]
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                ]
+            }
         ],
-        "timestamp": "2023-11-23T13:56:13.623892691"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:36:32.885078"
     },
-    "rs_gtf_transcript_gtf": {
+    "sarscov2 [bam] - forward strandedness + reference annotation - stub": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "strandedness": "reverse"
-                    },
-                    "test.transcripts.gtf:md5,bb346053a8c156b803b055133376c7fa"
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "forward"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
                 ]
-            ]
+            }
         ],
-        "timestamp": "2023-11-23T13:56:22.693599559"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:36:43.325777"
     },
-    "fs_gtf_abundance": {
+    "sarscov2 [bam] - reverse strandedness + reference annotation - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:37:06.085936"
+    },
+    "sarscov2 [bam] - reverse strandedness - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                ],
+                "abundance": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "ballgown": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coverage_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "transcript_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "strandedness": "reverse"
+                        },
+                        "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:36:53.837578"
+    },
+    "sarscov2 [bam] - reverse strandedness + reference annotation": {
         "content": [
             [
                 [
                     {
                         "id": "test",
-                        "strandedness": "forward"
+                        "strandedness": "reverse"
                     },
-                    "test.gene.abundance.txt:md5,7d8bce7f2a922e367cedccae7267c22e"
+                    "test.gene.abundance.txt:md5,7385b870b955dae2c2ab78a70cf05cce"
                 ]
-            ]
-        ],
-        "timestamp": "2023-11-23T13:56:00.482135418"
-    },
-    "rs_gtf_ballgown": {
-        "content": [
+            ],
             [
                 [
                     {
@@ -115,72 +455,54 @@
                         "t_data.ctab:md5,3b66c065da73ae0dd41cc332eff6a818"
                     ]
                 ]
-            ]
-        ],
-        "timestamp": "2023-11-23T13:56:22.715698347"
-    },
-    "rs_transcript_gtf": {
-        "content": [
+            ],
             [
                 [
                     {
                         "id": "test",
                         "strandedness": "reverse"
                     },
-                    "test.transcripts.gtf:md5,31c34aec2bf36bb0ea3c16c2afeeeb1f"
+                    "test.transcripts.gtf:md5,bb346053a8c156b803b055133376c7fa"
                 ]
-            ]
-        ],
-        "timestamp": "2023-11-23T13:56:13.590054035"
-    },
-    "rs_gtf_versions": {
-        "content": [
+            ],
             [
                 "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
             ]
         ],
-        "timestamp": "2023-11-23T13:56:22.725513476"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:34:03.114695"
     },
-    "fs_gtf_ballgown": {
+    "sarscov2 [bam] - reverse strandedness": {
         "content": [
             [
                 [
                     {
                         "id": "test",
-                        "strandedness": "forward"
+                        "strandedness": "reverse"
                     },
-                    [
-                        "e2t.ctab:md5,e981c0038295ae54b63cedb1083f1540",
-                        "e_data.ctab:md5,6b4cf69bc03f3f69890f972a0e8b7471",
-                        "i2t.ctab:md5,8a117c8aa4334b4c2d4711932b006fb4",
-                        "i_data.ctab:md5,be3abe09740603213f83d50dcf81427f",
-                        "t_data.ctab:md5,3b66c065da73ae0dd41cc332eff6a818"
-                    ]
+                    "test.gene.abundance.txt:md5,d6f5c8cadb8458f1df0427cf790246e3"
                 ]
-            ]
-        ],
-        "timestamp": "2023-11-23T13:56:00.494299817"
-    },
-    "fs_versions": {
-        "content": [
-            [
-                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
-            ]
-        ],
-        "timestamp": "2023-11-23T13:55:41.049417582"
-    },
-    "rs_gtf_abundance": {
-        "content": [
+            ],
             [
                 [
                     {
                         "id": "test",
                         "strandedness": "reverse"
                     },
-                    "test.gene.abundance.txt:md5,7385b870b955dae2c2ab78a70cf05cce"
+                    "test.transcripts.gtf:md5,31c34aec2bf36bb0ea3c16c2afeeeb1f"
                 ]
+            ],
+            [
+                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
             ]
         ],
-        "timestamp": "2023-11-23T13:56:22.701059059"
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T12:33:52.874479"
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/ucsc/gtftogenepred/environment.yml b/modules/nf-core/ucsc/gtftogenepred/environment.yml
index 5216fc8ff..5c4f6c2fa 100644
--- a/modules/nf-core/ucsc/gtftogenepred/environment.yml
+++ b/modules/nf-core/ucsc/gtftogenepred/environment.yml
@@ -1,7 +1,5 @@
-name: ucsc_gtftogenepred
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::ucsc-gtftogenepred=447
diff --git a/modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test b/modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test
new file mode 100644
index 000000000..e0396a63c
--- /dev/null
+++ b/modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test
@@ -0,0 +1,36 @@
+
+nextflow_process {
+
+    name "Test Process UCSC_GTFTOGENEPRED"
+    script "../main.nf"
+    process "UCSC_GTFTOGENEPRED"
+    config "./nextflow.config"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "ucsc"
+    tag "ucsc/gtftogenepred"
+
+    test("test-ucsc-gtftogenepred") {
+
+        when {
+            process {
+                """
+                input[0] = [
+				    [ id: 'test' ], // meta map
+				    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gtf', checkIfExists: true) ]
+				]
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test.snap b/modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test.snap
new file mode 100644
index 000000000..f021f8238
--- /dev/null
+++ b/modules/nf-core/ucsc/gtftogenepred/tests/main.nf.test.snap
@@ -0,0 +1,51 @@
+{
+    "test-ucsc-gtftogenepred": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.genepred:md5,779e4749efaf38da3443ddfde30cc76c"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.refflat:md5,4101802f41d4cf7ee2667587da11bf42"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,fd95365619a316eb451190365b1b799e"
+                ],
+                "genepred": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.genepred:md5,779e4749efaf38da3443ddfde30cc76c"
+                    ]
+                ],
+                "refflat": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.refflat:md5,4101802f41d4cf7ee2667587da11bf42"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,fd95365619a316eb451190365b1b799e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-23T08:55:50.58172"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/ucsc/gtftogenepred/tests/nextflow.config b/modules/nf-core/ucsc/gtftogenepred/tests/nextflow.config
new file mode 100644
index 000000000..889bb6ce6
--- /dev/null
+++ b/modules/nf-core/ucsc/gtftogenepred/tests/nextflow.config
@@ -0,0 +1,8 @@
+process {
+    withName: UCSC_GTFTOGENEPRED {
+        ext.args = [
+            "-genePredExt",
+            "-geneNameAsName2"
+        ].join(' ').trim()
+    }
+}

From 55dcc55b5cced7cd14d2802beee18e1b99ecc8b6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 Sep 2024 14:56:24 +0200
Subject: [PATCH 041/887] update arriba modules

---
 modules/nf-core/arriba/download/main.nf                    | 2 +-
 null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt | 1 +
 2 files changed, 2 insertions(+), 1 deletion(-)
 create mode 100644 null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt

diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index 5e606574c..96a4e3362 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -18,7 +18,7 @@ process ARRIBA_DOWNLOAD {
 
     script:
     """
-    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz --no-check-certificate
+    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz
     tar -xzvf arriba_v2.4.0.tar.gz
     rm arriba_v2.4.0.tar.gz
     mv arriba_v2.4.0/database/* .
diff --git a/null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt b/null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt
new file mode 100644
index 000000000..6b739acdf
--- /dev/null
+++ b/null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt
@@ -0,0 +1 @@
+task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar

From f9f808da200334fdd8378cf53719c2219097fc4e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 Sep 2024 15:23:30 +0200
Subject: [PATCH 042/887] rm file

---
 null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt | 1 -
 1 file changed, 1 deletion(-)
 delete mode 100644 null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt

diff --git a/null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt b/null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt
deleted file mode 100644
index 6b739acdf..000000000
--- a/null/pipeline_info/execution_trace_2024-09-12_11-34-43.txt
+++ /dev/null
@@ -1 +0,0 @@
-task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar

From 7f8ee685abc8261efe4d847cc5b6536ad1955f57 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 Sep 2024 15:57:50 +0200
Subject: [PATCH 043/887] fixes

---
 .../pipeline_info/execution_trace_2024-09-12_15-47-05.txt | 1 +
 .../pipeline_info/execution_trace_2024-09-12_15-47-37.txt | 1 +
 .../pipeline_info/execution_trace_2024-09-12_15-48-22.txt | 1 +
 workflows/build_references.nf                             | 8 +++-----
 4 files changed, 6 insertions(+), 5 deletions(-)
 create mode 100644 null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt
 create mode 100644 null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt
 create mode 100644 null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt

diff --git a/null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt b/null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt
new file mode 100644
index 000000000..6b739acdf
--- /dev/null
+++ b/null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt
@@ -0,0 +1 @@
+task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar
diff --git a/null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt b/null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt
new file mode 100644
index 000000000..6b739acdf
--- /dev/null
+++ b/null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt
@@ -0,0 +1 @@
+task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar
diff --git a/null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt b/null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt
new file mode 100644
index 000000000..6b739acdf
--- /dev/null
+++ b/null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt
@@ -0,0 +1 @@
+task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 7fdfcd8ca..da6664373 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -25,8 +25,6 @@ include { RRNATRANSCRIPTS }                 from '../modules/nf-core/rrnatranscr
 include { SAMTOOLS_FAIDX }                  from '../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../modules/nf-core/star/genomegenerate/main'
 include { UCSC_GTFTOGENEPRED }              from '../modules/nf-core/ucsc/gtftogenepred/main'
-include { GATK4_CREATESEQUENCEDICTIONARY }  from '../modules/nf-core/gatk4/createsequencedictionary/main'
-include { GATK4_BEDTOINTERVALLIST }         from '../modules/nf-core/gatk4/bedtointervallist/main'
 include { SALMON_INDEX }                    from '../modules/nf-core/salmon/index/main'
 include { GFFREAD }                         from '../modules/nf-core/gffread/main'
 
@@ -42,8 +40,8 @@ workflow BUILD_REFERENCES {
     fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
     ENSEMBL_DOWNLOAD( params.ensembl_version, params.genome, fake_meta )
     HGNC_DOWNLOAD()
-    SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.fasta, [[],[]])
-    GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.fasta)
+    SAMTOOLS_FAIDX(ENSEMBL_DOWNLOAD.out.primary_assembly, [[],[]])
+    GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.primary_assembly)
 
 
     RRNATRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf.map{ meta, gtf -> [ gtf ] })
@@ -77,7 +75,7 @@ workflow BUILD_REFERENCES {
         UCSC_GTFTOGENEPRED(ENSEMBL_DOWNLOAD.out.chrgtf)
     } else {
         UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.chrgtf)
-
+    }
     if (params.fusionreport || params.all) {
         FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
     }

From e54c5a67c980ef3c9d532ed311b9256767622173 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 12 Sep 2024 15:58:15 +0200
Subject: [PATCH 044/887] remove files

---
 null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt | 1 -
 null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt | 1 -
 null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt | 1 -
 3 files changed, 3 deletions(-)
 delete mode 100644 null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt
 delete mode 100644 null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt
 delete mode 100644 null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt

diff --git a/null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt b/null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt
deleted file mode 100644
index 6b739acdf..000000000
--- a/null/pipeline_info/execution_trace_2024-09-12_15-47-05.txt
+++ /dev/null
@@ -1 +0,0 @@
-task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar
diff --git a/null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt b/null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt
deleted file mode 100644
index 6b739acdf..000000000
--- a/null/pipeline_info/execution_trace_2024-09-12_15-47-37.txt
+++ /dev/null
@@ -1 +0,0 @@
-task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar
diff --git a/null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt b/null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt
deleted file mode 100644
index 6b739acdf..000000000
--- a/null/pipeline_info/execution_trace_2024-09-12_15-48-22.txt
+++ /dev/null
@@ -1 +0,0 @@
-task_id	hash	native_id	name	status	exit	submit	duration	realtime	%cpu	peak_rss	peak_vmem	rchar	wchar

From 26d44e44a00e51c4cd3640a43e5cbbb5df938c27 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Sep 2024 10:52:02 +0200
Subject: [PATCH 045/887] update new fastq arg

---
 subworkflows/local/trim_workflow.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/trim_workflow.nf b/subworkflows/local/trim_workflow.nf
index ea21134db..27088a180 100644
--- a/subworkflows/local/trim_workflow.nf
+++ b/subworkflows/local/trim_workflow.nf
@@ -13,7 +13,7 @@ workflow TRIM_WORKFLOW {
         ch_fastqc_trimmed = Channel.empty()
 
         if (params.fastp_trim) {
-            FASTP(reads, params.adapter_fasta, false, false)
+            FASTP(reads, params.adapter_fasta, false, false, false)
             ch_versions = ch_versions.mix(FASTP.out.versions)
 
             FASTQC_FOR_FASTP(FASTP.out.reads)

From 9a4a8eb0e1603e3749b6b7366aefc9dca06f0d0f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Sep 2024 14:25:51 +0200
Subject: [PATCH 046/887] update multiqc and multiqc args

---
 modules.json                                    | 2 +-
 modules/nf-core/multiqc/environment.yml         | 2 +-
 modules/nf-core/multiqc/main.nf                 | 4 ++--
 modules/nf-core/multiqc/tests/main.nf.test.snap | 6 +++---
 workflows/rnafusion.nf                          | 4 +++-
 5 files changed, 10 insertions(+), 8 deletions(-)

diff --git a/modules.json b/modules.json
index 2b34aabb8..322f834fa 100644
--- a/modules.json
+++ b/modules.json
@@ -67,7 +67,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "7c316cae26baf55e0add993bed2b0c9f7105c653",
                         "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index f1cd99b07..0fe126444 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.24.1
+  - bioconda::multiqc=1.25
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index ceaec139e..b9ccebdbb 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.24.1--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.24.1--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.25--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.25--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index 83fa080c8..b779e4692 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,7 +2,7 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760"
+                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
             ]
         ],
         "meta": {
@@ -17,7 +17,7 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760"
+                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
             ]
         ],
         "meta": {
@@ -29,7 +29,7 @@
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760"
+                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
             ]
         ],
         "meta": {
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 85bde3955..d73ac21ed 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -274,7 +274,9 @@ workflow RNAFUSION {
         ch_multiqc_files.collect(),
         ch_multiqc_config.toList(),
         ch_multiqc_custom_config.toList(),
-        ch_multiqc_logo.toList()
+        ch_multiqc_logo.toList(),
+        [],
+        []
     )
 
     emit:

From eb6e354c180f6c533cbb862678403984e79275b2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 23 Sep 2024 21:15:42 +0200
Subject: [PATCH 047/887] change strategy

---
 conf/modules.config                    |  3 ++-
 modules/local/ensembl/main.nf          | 22 +++++++++--------
 nextflow.config                        |  2 +-
 subworkflows/local/build_references.nf |  7 +++++-
 workflows/rnafusion.nf                 | 34 ++++++++++++++++++++++++--
 5 files changed, 53 insertions(+), 15 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index e78f73dbb..bbafa80d6 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -49,7 +49,8 @@ process {
         ext.when   = { !params.build_references }
     }
 
-    withName: 'ENSEMBL_DOWNLOAD' {
+    withName: '.*BUILD_REFERENCES:ENSEMBL_DOWNLOAD' {
+        ext.when = { !params.fasta || ! params.gtf }
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },
             mode: params.publish_dir_mode,
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 8297e2901..695ab3123 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -14,20 +14,23 @@ process ENSEMBL_DOWNLOAD {
 
     output:
     tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.gtf")                       , emit: gtf
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa")   , emit: primary_assembly
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.chr.gtf")                   , emit: chrgtf
+    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa")   , emit: fasta
     path "versions.yml"                                                                          , emit: versions
 
 
     script:
     """
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.primary_assembly.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.dna.primary_assembly.fa.gz
-    wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
+    if [ ${genome} == 'GRCh37' ]; then
+        wget ftp://ftp.ensembl.org/pub/grch37/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${genome}.87.gtf.gz -O Homo_sapiens.${genome}.${ensembl_version}.gtf.gz
+        wget ftp://ftp.ensembl.org/pub/grch37/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${genome}.dna.primary_assembly.fa.gz -O Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz
 
-    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz
-    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.dna.primary_assembly.fa.gz
-    gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz
+
+    else:
+        wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${genome}.${ensembl_version}.gtf.gz
+        wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${genome}.dna.primary_assembly.fa.gz -O Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz
+
+    gunzip Homo_sapiens.${genome}.${ensembl_version}.gtf.gz
+    gunzip Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -40,8 +43,7 @@ process ENSEMBL_DOWNLOAD {
     stub:
     """
     touch "Homo_sapiens.${genome}.${ensembl_version}.gtf"
-    touch "Homo_sapiens.${params.genome}.${ensembl_version}.dna.primary_assembly.fa"
-    touch "Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf"
+    touch "Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa"
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/nextflow.config b/nextflow.config
index b7d78f7d9..ccaf0348a 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -27,7 +27,7 @@ params {
     // Genome
     genome                     = 'GRCh38'
     genomes_base               = "${params.outdir}/references"
-    ensembl_version            = 112
+    ensembl_version            = 111
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index a1a640509..c7a62033e 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -35,12 +35,17 @@ include { GFFREAD }                         from '../../modules/nf-core/gffread/
 */
 
 workflow BUILD_REFERENCES {
-
+    take:
+        genome                           // channel: [mandatory]  val(genome)
+        ensembl_version                  // channel: [mandatory]  val(ensembl_version)
 
     main:
     ch_versions = Channel.empty()
 
 
+
+
+
     if (!file(params.fasta).exists() || file(params.fasta).isEmpty() ||
             !file(params.chrgtf).exists() || file(params.chrgtf).isEmpty() ||
             !file(params.gtf).exists() || file(params.gtf).isEmpty()){
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 302f2829f..368658401 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -65,8 +65,38 @@ workflow RNAFUSION {
     // Create references if necessary
     //
 
-    BUILD_REFERENCES()
-
+    BUILD_REFERENCES(params.genome, params.ensembl_version)
+    ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
+
+    // Optional
+    ch_fasta                      = params.fasta                        ? Channel.fromPath(params.fasta).map {it -> [[id:it[0].simpleName], it]}.collect()
+                                                                        : BUILD_REFERENCES.out.fasta.map {it -> [[id:it[0].simpleName], it]}.collect()
+    ch_gtf                        = params.gtf                          ? Channel.fromPath(params.gtf).map {it -> [[id:it[0].simpleName], it]}.collect()
+                                                                        : downloads.gtf.map {it -> [[id:it[0].simpleName], it]}.collect()
+    ch_vep_cache_unprocessed      = params.vep_cache                    ? Channel.fromPath(params.vep_cache)
+                                                                        : Channel.empty().mix(downloads.vep_cache)
+    ch_vep_extra_files_unsplit    = params.vep_plugin_files             ? Channel.fromPath(params.vep_plugin_files)
+                                                                        : Channel.empty().mix(downloads.vep_plugin)
+    ch_fai                        = params.fai                          ? Channel.fromPath(params.fai).map {it -> [[id:it[0].simpleName], it]}.collect()
+                                                                        : Channel.empty()
+    ch_gene_panel_clinical_filter = params.gene_panel_clinical_filter   ? Channel.fromPath(params.gene_panel_clinical_filter).collect()
+                                                                        : Channel.empty()
+    ch_ref_drop_annot_file        = params.reference_drop_annot_file    ? Channel.fromPath(params.reference_drop_annot_file).collect()
+                                                                        : Channel.empty()
+    ch_ref_drop_count_file        = params.reference_drop_count_file    ? Channel.fromPath(params.reference_drop_count_file).collect()
+                                                                        : Channel.empty()
+    ch_ref_drop_splice_folder     = params.reference_drop_splice_folder ? Channel.fromPath(params.reference_drop_splice_folder).collect()
+                                                                        : Channel.empty()
+    ch_salmon_index               = params.salmon_index                 ? Channel.fromPath(params.salmon_index)
+                                                                        : Channel.empty()
+    ch_star_index                 = params.star_index                   ? Channel.fromPath(params.star_index).map {it -> [[id:it[0].simpleName], it]}.collect()
+                                                                        : Channel.empty()
+    ch_transcript_fasta           = params.transcript_fasta             ? Channel.fromPath(params.transcript_fasta)
+                                                                        : Channel.empty()
+    ch_sequence_dict              = params.sequence_dict                ? Channel.fromPath(params.sequence_dict).map{ it -> [[id:it[0].simpleName], it] }.collect()
+                                                                        : Channel.empty()
+    ch_subsample_bed              = params.subsample_bed                ? Channel.fromPath(params.subsample_bed).collect()
+                                                                        : Channel.empty()
 
     //
     // Create channel from input file provided through params.input

From 40fbd83d3d45d009af01dda1eea44ef9af44fe24 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 7 Oct 2024 14:41:49 +0200
Subject: [PATCH 048/887] intermediate state

---
 modules/local/fusioncatcher/download/main.nf |   5 +-
 modules/local/starfusion/download/main.nf    |   2 +-
 nextflow.config                              |   3 +-
 subworkflows/local/build_references.nf       |  63 ++++---
 workflows/rnafusion.nf                       | 170 +++++++++----------
 5 files changed, 121 insertions(+), 122 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index aec162882..916b4673a 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -20,9 +20,10 @@ process FUSIONCATCHER_DOWNLOAD {
 
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
-    def url =
+        // TODO: move to my sourceforge
+
+    // def url =
     """
-    TODO: move to my sourceforge
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.aa
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ab
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ac
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index e3a4b1b85..fedadfa0b 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -9,7 +9,7 @@ process STARFUSION_DOWNLOAD {
     path "ctat_genome_lib_build_dir/ref_annot.gtf", emit: chrgtf
 
 
-    TODO: move to my sourceforge
+    // TODO: move to my sourceforge
     script:
     """
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.10/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
diff --git a/nextflow.config b/nextflow.config
index ccaf0348a..db5a3f633 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -27,7 +27,7 @@ params {
     // Genome
     genome                     = 'GRCh38'
     genomes_base               = "${params.outdir}/references"
-    ensembl_version            = 111
+    ensembl_version            = 112
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
@@ -72,7 +72,6 @@ params {
     fasta                         = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa"
     fai                           = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa.fai"
     gtf                           = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
-    chrgtf                        = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf"
     refflat                       = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf.refflat"
     rrna_intervals                = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list"
     ensembl_ref                   = "${params.genomes_base}/ensembl"
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index c7a62033e..cfb4258ba 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -42,25 +42,26 @@ workflow BUILD_REFERENCES {
     main:
     ch_versions = Channel.empty()
 
-
-
-
-
     if (!file(params.fasta).exists() || file(params.fasta).isEmpty() ||
-            !file(params.chrgtf).exists() || file(params.chrgtf).isEmpty() ||
             !file(params.gtf).exists() || file(params.gtf).isEmpty()){
         fake_meta = [:]
         fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
         ENSEMBL_DOWNLOAD(params.ensembl_version, params.genome, fake_meta)
+        ch_versions = ENSEMBL_DOWNLOAD.out.versions
         ch_fasta = ENSEMBL_DOWNLOAD.out.primary_assembly
-        ch_chrgtf = ENSEMBL_DOWNLOAD.out.chrgtf
         ch_gtf = ENSEMBL_DOWNLOAD.out.gtf
     } else {
         ch_fasta = Channel.fromPath(params.fasta).map { that -> [[id:that.Name], that] }
-        ch_chrgtf = Channel.fromPath(params.chrgtf).map { that -> [[id:that.Name], that] }
         ch_gtf = Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }
     }
 
+    if (!file(params.fai).exists() || file(params.fai).isEmpty()){
+        SAMTOOLS_FAIDX(ch_fasta, [[],[]]).fai
+        ch_versions = SAMTOOLS_FAIDX.out.versions
+        ch_fai = SAMTOOLS_FAIDX.out.fai
+    } else {
+        ch_fai = Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
+    }
 
     if ((!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
             !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()) && !params.skip_vcf){
@@ -72,10 +73,6 @@ workflow BUILD_REFERENCES {
         ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
     }
 
-
-    ch_fai = (!file(params.fai).exists() || file(params.fai).isEmpty()) ? SAMTOOLS_FAIDX(ch_fasta, [[],[]]).fai : Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
-
-
     if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty()){
         GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
         RRNA_TRANSCRIPTS(ch_gtf)
@@ -86,9 +83,12 @@ workflow BUILD_REFERENCES {
         ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
     }
 
-
-    ch_refflat = (!file(params.refflat).exists() || file(params.refflat).isEmpty()) ? GTF_TO_REFFLAT.refflat : Channel.fromPath(params.refflat)
-
+    if (!file(params.refflat).exists() || file(params.refflat).isEmpty()){
+        GTF_TO_REFFLAT(ch_gtf)
+        ch_refflat = GTF_TO_REFFLAT.out.refflat
+    } else {
+        ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
+    }
 
     if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty() ||
         !file(params.salmon_index_stub_check).exists() || file(params.salmon_index_stub_check).isEmpty()){ // add condition for qc
@@ -99,7 +99,6 @@ workflow BUILD_REFERENCES {
         ch_salmon_index = Channel.fromPath(params.salmon_index)
     }
 
-
     if ((params.starindex || params.all || params.starfusion || params.arriba) &&
             (!file(params.starindex_ref).exists() || file(params.starindex_ref).isEmpty() ||
             !file(params.starindex_ref_stub_check).exists() || file(params.starindex_ref_stub_check).isEmpty() )) {
@@ -109,18 +108,17 @@ workflow BUILD_REFERENCES {
         ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
     }
 
-
-    if ((params.arriba || params.all) &&
-            (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
-            !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
-            !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
-        ARRIBA_DOWNLOAD()
-        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
-        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
-        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
-    } else {
-// TODO need to update the module to emit blacklist,knownfusions etc
-    }
+//     if ((params.arriba || params.all) &&
+//             (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
+//             !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
+//             !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
+//         ARRIBA_DOWNLOAD()
+//         ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
+//         ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
+//         ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
+//     } else {
+// // TODO need to update the module to emit blacklist,knownfusions etc
+//     }
 
 
     if ((params.fusioncatcher || params.all) &&
@@ -159,21 +157,22 @@ workflow BUILD_REFERENCES {
 
     emit:
     ch_fasta
-    ch_chrgtf
     ch_gtf
+    ch_fai
+
     ch_hgnc_ref
     ch_hgnc_date
-    ch_fai
     ch_rrna_interval
     ch_refflat
     ch_salmon_index
     ch_starindex_ref
-    ch_arriba_ref_blacklist
-    ch_arriba_ref_known_fusions
-    ch_arriba_ref_protein_domains
+    // ch_arriba_ref_blacklist
+    // ch_arriba_ref_known_fusions
+    // ch_arriba_ref_protein_domains
     ch_fusioncatcher_ref
     ch_starfusion_ref
     ch_fusionreport_ref
+    versions        = ch_versions
 }
 
 /*
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 368658401..67dedb4b1 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -68,95 +68,95 @@ workflow RNAFUSION {
     BUILD_REFERENCES(params.genome, params.ensembl_version)
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
-    // Optional
-    ch_fasta                      = params.fasta                        ? Channel.fromPath(params.fasta).map {it -> [[id:it[0].simpleName], it]}.collect()
-                                                                        : BUILD_REFERENCES.out.fasta.map {it -> [[id:it[0].simpleName], it]}.collect()
-    ch_gtf                        = params.gtf                          ? Channel.fromPath(params.gtf).map {it -> [[id:it[0].simpleName], it]}.collect()
-                                                                        : downloads.gtf.map {it -> [[id:it[0].simpleName], it]}.collect()
-    ch_vep_cache_unprocessed      = params.vep_cache                    ? Channel.fromPath(params.vep_cache)
-                                                                        : Channel.empty().mix(downloads.vep_cache)
-    ch_vep_extra_files_unsplit    = params.vep_plugin_files             ? Channel.fromPath(params.vep_plugin_files)
-                                                                        : Channel.empty().mix(downloads.vep_plugin)
-    ch_fai                        = params.fai                          ? Channel.fromPath(params.fai).map {it -> [[id:it[0].simpleName], it]}.collect()
-                                                                        : Channel.empty()
-    ch_gene_panel_clinical_filter = params.gene_panel_clinical_filter   ? Channel.fromPath(params.gene_panel_clinical_filter).collect()
-                                                                        : Channel.empty()
-    ch_ref_drop_annot_file        = params.reference_drop_annot_file    ? Channel.fromPath(params.reference_drop_annot_file).collect()
-                                                                        : Channel.empty()
-    ch_ref_drop_count_file        = params.reference_drop_count_file    ? Channel.fromPath(params.reference_drop_count_file).collect()
-                                                                        : Channel.empty()
-    ch_ref_drop_splice_folder     = params.reference_drop_splice_folder ? Channel.fromPath(params.reference_drop_splice_folder).collect()
-                                                                        : Channel.empty()
-    ch_salmon_index               = params.salmon_index                 ? Channel.fromPath(params.salmon_index)
-                                                                        : Channel.empty()
-    ch_star_index                 = params.star_index                   ? Channel.fromPath(params.star_index).map {it -> [[id:it[0].simpleName], it]}.collect()
-                                                                        : Channel.empty()
-    ch_transcript_fasta           = params.transcript_fasta             ? Channel.fromPath(params.transcript_fasta)
-                                                                        : Channel.empty()
-    ch_sequence_dict              = params.sequence_dict                ? Channel.fromPath(params.sequence_dict).map{ it -> [[id:it[0].simpleName], it] }.collect()
-                                                                        : Channel.empty()
-    ch_subsample_bed              = params.subsample_bed                ? Channel.fromPath(params.subsample_bed).collect()
-                                                                        : Channel.empty()
+    // // Optional
+    // ch_fasta                      = params.fasta                        ? Channel.fromPath(params.fasta).map {it -> [[id:it[0].simpleName], it]}.collect()
+    //                                                                     : BUILD_REFERENCES.out.fasta.map {it -> [[id:it[0].simpleName], it]}.collect()
+    // ch_gtf                        = params.gtf                          ? Channel.fromPath(params.gtf).map {it -> [[id:it[0].simpleName], it]}.collect()
+    //                                                                     : downloads.gtf.map {it -> [[id:it[0].simpleName], it]}.collect()
+    // ch_vep_cache_unprocessed      = params.vep_cache                    ? Channel.fromPath(params.vep_cache)
+    //                                                                     : Channel.empty().mix(downloads.vep_cache)
+    // ch_vep_extra_files_unsplit    = params.vep_plugin_files             ? Channel.fromPath(params.vep_plugin_files)
+    //                                                                     : Channel.empty().mix(downloads.vep_plugin)
+    // ch_fai                        = params.fai                          ? Channel.fromPath(params.fai).map {it -> [[id:it[0].simpleName], it]}.collect()
+    //                                                                     : Channel.empty()
+    // ch_gene_panel_clinical_filter = params.gene_panel_clinical_filter   ? Channel.fromPath(params.gene_panel_clinical_filter).collect()
+    //                                                                     : Channel.empty()
+    // ch_ref_drop_annot_file        = params.reference_drop_annot_file    ? Channel.fromPath(params.reference_drop_annot_file).collect()
+    //                                                                     : Channel.empty()
+    // ch_ref_drop_count_file        = params.reference_drop_count_file    ? Channel.fromPath(params.reference_drop_count_file).collect()
+    //                                                                     : Channel.empty()
+    // ch_ref_drop_splice_folder     = params.reference_drop_splice_folder ? Channel.fromPath(params.reference_drop_splice_folder).collect()
+    //                                                                     : Channel.empty()
+    // ch_salmon_index               = params.salmon_index                 ? Channel.fromPath(params.salmon_index)
+    //                                                                     : Channel.empty()
+    // ch_star_index                 = params.star_index                   ? Channel.fromPath(params.star_index).map {it -> [[id:it[0].simpleName], it]}.collect()
+    //                                                                     : Channel.empty()
+    // ch_transcript_fasta           = params.transcript_fasta             ? Channel.fromPath(params.transcript_fasta)
+    //                                                                     : Channel.empty()
+    // ch_sequence_dict              = params.sequence_dict                ? Channel.fromPath(params.sequence_dict).map{ it -> [[id:it[0].simpleName], it] }.collect()
+    //                                                                     : Channel.empty()
+    // ch_subsample_bed              = params.subsample_bed                ? Channel.fromPath(params.subsample_bed).collect()
+    //                                                                     : Channel.empty()
 
     //
     // Create channel from input file provided through params.input
     //
-    Channel
-        .fromSamplesheet("input")
-        .map {
-            meta, fastq_1, fastq_2, strandedness ->
-                if (!fastq_2) {
-                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
-                } else {
-                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
-                }
-        }
-        .groupTuple()
-        .map {
-            validateInputSamplesheet(it)
-        }
-        .branch {
-            meta, fastqs ->
-                single  : fastqs.size() == 1
-                    return [ meta, fastqs.flatten() ]
-                multiple: fastqs.size() > 1
-                    return [ meta, fastqs.flatten() ]
-        }
-        .set { ch_fastq }
-
-    //
-    // MODULE: Concatenate FastQ files from same sample if required
-    //
-    CAT_FASTQ (
-        ch_fastq.multiple
-    )
-    .reads
-    .mix(ch_fastq.single)
-    .set { ch_cat_fastq }
-    ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
-
-
-    //
-    // QC from FASTQ files
-    //
-    FASTQC (
-        ch_cat_fastq
-    )
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    ch_versions = ch_versions.mix(FASTQC.out.versions)
-
-    SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
-    ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.zip.collect{it[1]})
-    ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
-
-    //
-    // Trimming
-    //
-    TRIM_WORKFLOW (
-        ch_cat_fastq
-    )
-    ch_reads = TRIM_WORKFLOW.out.trimmed_reads
-    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+    // Channel
+    //     .fromSamplesheet("input")
+    //     .map {
+    //         meta, fastq_1, fastq_2, strandedness ->
+    //             if (!fastq_2) {
+    //                 return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
+    //             } else {
+    //                 return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
+    //             }
+    //     }
+    //     .groupTuple()
+    //     .map {
+    //         validateInputSamplesheet(it)
+    //     }
+    //     .branch {
+    //         meta, fastqs ->
+    //             single  : fastqs.size() == 1
+    //                 return [ meta, fastqs.flatten() ]
+    //             multiple: fastqs.size() > 1
+    //                 return [ meta, fastqs.flatten() ]
+    //     }
+    //     .set { ch_fastq }
+
+    // //
+    // // MODULE: Concatenate FastQ files from same sample if required
+    // //
+    // CAT_FASTQ (
+    //     ch_fastq.multiple
+    // )
+    // .reads
+    // .mix(ch_fastq.single)
+    // .set { ch_cat_fastq }
+    // ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
+
+
+    // //
+    // // QC from FASTQ files
+    // //
+    // FASTQC (
+    //     ch_cat_fastq
+    // )
+    // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+    // ch_versions = ch_versions.mix(FASTQC.out.versions)
+
+    // SALMON_QUANT( ch_reads_all, BUILD_REFERENCES.out.ch_salmon_index.map{ meta, index ->  index  }, BUILD_REFERENCES.out.ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+    // ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.zip.collect{it[1]})
+    // ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
+
+    // //
+    // // Trimming
+    // //
+    // TRIM_WORKFLOW (
+    //     ch_cat_fastq
+    // )
+    // ch_reads = TRIM_WORKFLOW.out.trimmed_reads
+    // ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
 
 //     //

From e5ffe10759df03a754747d7c6b3b51fa3be2aabc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 7 Oct 2024 14:43:45 +0200
Subject: [PATCH 049/887] update

---
 modules.json                                  |  56 +--
 .../nf-core/agat/convertspgff2tsv/meta.yml    |  40 ++-
 modules/nf-core/arriba/arriba/meta.yml        | 187 +++++-----
 modules/nf-core/arriba/download/meta.yml      |  21 +-
 modules/nf-core/bedops/convert2bed/meta.yml   |  49 ++-
 modules/nf-core/cat/cat/meta.yml              |  39 ++-
 modules/nf-core/cat/fastq/meta.yml            |  43 +--
 modules/nf-core/fastp/meta.yml                | 138 +++++---
 modules/nf-core/fastqc/meta.yml               |  57 +--
 .../nf-core/gatk4/bedtointervallist/meta.yml  |  59 ++--
 .../gatk4/createsequencedictionary/meta.yml   |  37 +-
 .../gatk4/markduplicates/environment.yml      |   3 +-
 modules/nf-core/gatk4/markduplicates/meta.yml | 121 ++++---
 modules/nf-core/gffread/meta.yml              |  84 +++--
 modules/nf-core/multiqc/environment.yml       |   2 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 modules/nf-core/multiqc/meta.yml              |  91 ++---
 .../nf-core/multiqc/tests/main.nf.test.snap   |  26 +-
 .../picard/collectinsertsizemetrics/meta.yml  |  59 ++--
 .../picard/collectrnaseqmetrics/meta.yml      |  75 ++--
 .../nf-core/picard/collectwgsmetrics/meta.yml |  97 +++---
 modules/nf-core/rrnatranscripts/meta.yml      |  25 +-
 modules/nf-core/salmon/index/meta.yml         |  27 +-
 modules/nf-core/salmon/quant/meta.yml         |  94 +++--
 .../nf-core/samtools/faidx/environment.yml    |   7 +-
 modules/nf-core/samtools/faidx/main.nf        |   4 +-
 modules/nf-core/samtools/faidx/meta.yml       |  85 +++--
 .../samtools/faidx/tests/main.nf.test.snap    |  50 +--
 .../nf-core/samtools/index/environment.yml    |   6 +-
 modules/nf-core/samtools/index/main.nf        |   4 +-
 modules/nf-core/samtools/index/meta.yml       |  68 ++--
 .../samtools/index/tests/main.nf.test.snap    |  46 +--
 modules/nf-core/samtools/sort/environment.yml |   6 +-
 modules/nf-core/samtools/sort/main.nf         |   5 +-
 modules/nf-core/samtools/sort/meta.yml        |  99 +++---
 .../samtools/sort/tests/main.nf.test.snap     |  30 +-
 modules/nf-core/samtools/view/environment.yml |   6 +-
 modules/nf-core/samtools/view/main.nf         |   4 +-
 modules/nf-core/samtools/view/meta.yml        | 163 +++++----
 .../samtools/view/tests/main.nf.test.snap     |  66 ++--
 modules/nf-core/star/align/environment.yml    |   5 +-
 modules/nf-core/star/align/main.nf            |  33 +-
 modules/nf-core/star/align/meta.yml           | 258 +++++++++-----
 .../star/align/tests/main.nf.test.snap        | 324 ++++++++++--------
 .../star/align/tests/nextflow.arriba.config   |   3 -
 .../nf-core/star/align/tests/nextflow.config  |   3 -
 .../align/tests/nextflow.starfusion.config    |   3 -
 .../star/genomegenerate/environment.yml       |   3 +-
 modules/nf-core/star/genomegenerate/meta.yml  |  57 +--
 modules/nf-core/stringtie/merge/meta.yml      |  38 +-
 modules/nf-core/stringtie/stringtie/meta.yml  |  89 +++--
 modules/nf-core/ucsc/gtftogenepred/meta.yml   |  55 +--
 52 files changed, 1723 insertions(+), 1231 deletions(-)

diff --git a/modules.json b/modules.json
index 322f834fa..1a90927b5 100644
--- a/modules.json
+++ b/modules.json
@@ -7,142 +7,142 @@
                 "nf-core": {
                     "agat/convertspgff2tsv": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "bedops/convert2bed": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "7c316cae26baf55e0add993bed2b0c9f7105c653",
+                        "git_sha": "b8d36829fa84b6e404364abff787e8b07f6d058c",
                         "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "rrnatranscripts": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
                         "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
                         "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
                         "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
                         "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "30a97c755895b7dfe40a730b0695c554a10f1cdd",
                         "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
-                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/agat/convertspgff2tsv/meta.yml b/modules/nf-core/agat/convertspgff2tsv/meta.yml
index 83078787b..31f098683 100644
--- a/modules/nf-core/agat/convertspgff2tsv/meta.yml
+++ b/modules/nf-core/agat/convertspgff2tsv/meta.yml
@@ -9,31 +9,39 @@ keywords:
   - tsv
 tools:
   - agat:
-      description: "AGAT is a toolkit for manipulation and getting information from GFF/GTF files"
+      description: "AGAT is a toolkit for manipulation and getting information from
+        GFF/GTF files"
       homepage: "https://github.com/NBISweden/AGAT"
       documentation: "https://agat.readthedocs.io/"
       tool_dev_url: "https://github.com/NBISweden/AGAT"
       doi: "10.5281/zenodo.3552717"
       licence: ["GPL v3"]
+      identifier: biotools:AGAT
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - gff:
-      type: file
-      description: Annotation file in GFF3/GTF format
-      pattern: "*.{gff, gtf}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - gff:
+        type: file
+        description: Annotation file in GFF3/GTF format
+        pattern: "*.{gff, gtf}"
 output:
   - tsv:
-      type: file
-      description: Annotation file in TSV format
-      pattern: "*.{gtf}"
+      - meta:
+          type: file
+          description: Annotation file in TSV format
+          pattern: "*.{gtf}"
+      - "*.tsv":
+          type: file
+          description: Annotation file in TSV format
+          pattern: "*.{gtf}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@rannick"
 maintainers:
diff --git a/modules/nf-core/arriba/arriba/meta.yml b/modules/nf-core/arriba/arriba/meta.yml
index 11f744b54..f230dda1f 100644
--- a/modules/nf-core/arriba/arriba/meta.yml
+++ b/modules/nf-core/arriba/arriba/meta.yml
@@ -1,5 +1,6 @@
 name: arriba_arriba
-description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
+description: Arriba is a command-line tool for the detection of gene fusions from
+  RNA-Seq data.
 keywords:
   - fusion
   - arriba
@@ -13,97 +14,107 @@ tools:
       tool_dev_url: https://github.com/suhrig/arriba
       doi: "10.1101/gr.257246.119"
       licence: ["MIT"]
+      identifier: biotools:Arriba
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fasta:
-      type: file
-      description: Assembly FASTA file
-      pattern: "*.{fasta}"
-  - meta3:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - gtf:
-      type: file
-      description: Annotation GTF file
-      pattern: "*.{gtf}"
-  - meta4:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - blacklist:
-      type: file
-      description: Blacklist file
-      pattern: "*.{tsv}"
-  - meta5:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - known_fusions:
-      type: file
-      description: Known fusions file
-      pattern: "*.{tsv}"
-  - meta6:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - structural_variants:
-      type: file
-      description: Structural variants file
-      pattern: "*.{tsv}"
-  - meta7:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - tags:
-      type: file
-      description: Tags file
-      pattern: "*.{tsv}"
-  - meta8:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - protein_domains:
-      type: file
-      description: Protein domains file
-      pattern: "*.{gff3}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - fasta:
+        type: file
+        description: Assembly FASTA file
+        pattern: "*.{fasta}"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - gtf:
+        type: file
+        description: Annotation GTF file
+        pattern: "*.{gtf}"
+  - - meta4:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - blacklist:
+        type: file
+        description: Blacklist file
+        pattern: "*.{tsv}"
+  - - meta5:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - known_fusions:
+        type: file
+        description: Known fusions file
+        pattern: "*.{tsv}"
+  - - meta6:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - structural_variants:
+        type: file
+        description: Structural variants file
+        pattern: "*.{tsv}"
+  - - meta7:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - tags:
+        type: file
+        description: Tags file
+        pattern: "*.{tsv}"
+  - - meta8:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - protein_domains:
+        type: file
+        description: Protein domains file
+        pattern: "*.{gff3}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
   - fusions:
-      type: file
-      description: File contains fusions which pass all of Arriba's filters.
-      pattern: "*.{fusions.tsv}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fusions.tsv":
+          type: file
+          description: File contains fusions which pass all of Arriba's filters.
+          pattern: "*.{fusions.tsv}"
   - fusions_fail:
-      type: file
-      description: File contains fusions that Arriba classified as an artifact or that are also observed in healthy tissue.
-      pattern: "*.{fusions.discarded.tsv}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fusions.discarded.tsv":
+          type: file
+          description: File contains fusions that Arriba classified as an artifact or
+            that are also observed in healthy tissue.
+          pattern: "*.{fusions.discarded.tsv}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@praveenraj2018"
   - "@rannick"
diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
index d32106163..531c66c2f 100644
--- a/modules/nf-core/arriba/download/meta.yml
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -1,5 +1,6 @@
 name: arriba_download
-description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
+description: Arriba is a command-line tool for the detection of gene fusions from
+  RNA-Seq data.
 keywords:
   - fusion
   - arriba
@@ -12,16 +13,18 @@ tools:
       tool_dev_url: https://github.com/suhrig/arriba
       doi: "10.1101/gr.257246.119"
       licence: ["MIT"]
+      identifier: biotools:Arriba
 
 output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
   - reference:
-      type: directory
-      description: Folder with arriba references
-      pattern: "*"
-
+      - "*":
+          type: directory
+          description: Folder with arriba references
+          pattern: "*"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@peterpru"
diff --git a/modules/nf-core/bedops/convert2bed/meta.yml b/modules/nf-core/bedops/convert2bed/meta.yml
index eea919650..6d84c0316 100644
--- a/modules/nf-core/bedops/convert2bed/meta.yml
+++ b/modules/nf-core/bedops/convert2bed/meta.yml
@@ -12,36 +12,33 @@ tools:
       tool_dev_url: "https://github.com/bedops"
       doi: "10.1093/bioinformatics/bts277"
       licence: ["GNU v2"]
+      identifier: biotools:bedops
 
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. `[ id:'sample1', single_end:false ]`
-
-  - input:
-      type: file
-      description: Sorted BAM/GFF/GTF/GVF/PSL file
-      pattern: "*.{bam,gff,gtf,gvf,psl}"
-
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - in_file:
+        type: file
+        description: Input file
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. `[ id:'sample1', single_end:false ]`
-
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
   - bed:
-      type: file
-      description: Sorted BED file
-      pattern: "*.{bed}"
-
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.bed":
+          type: file
+          description: Sorted BED file
+          pattern: "*.{bed}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@rannick"
 maintainers:
diff --git a/modules/nf-core/cat/cat/meta.yml b/modules/nf-core/cat/cat/meta.yml
index 00a8db0bc..81778a067 100644
--- a/modules/nf-core/cat/cat/meta.yml
+++ b/modules/nf-core/cat/cat/meta.yml
@@ -9,25 +9,32 @@ tools:
       description: Just concatenation
       documentation: https://man7.org/linux/man-pages/man1/cat.1.html
       licence: ["GPL-3.0-or-later"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - files_in:
-      type: file
-      description: List of compressed / uncompressed files
-      pattern: "*"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - files_in:
+        type: file
+        description: List of compressed / uncompressed files
+        pattern: "*"
 output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
   - file_out:
-      type: file
-      description: Concatenated file. Will be gzipped if file_out ends with ".gz"
-      pattern: "${file_out}"
+      - meta:
+          type: file
+          description: Concatenated file. Will be gzipped if file_out ends with ".gz"
+          pattern: "${file_out}"
+      - ${prefix}:
+          type: file
+          description: Concatenated file. Will be gzipped if file_out ends with ".gz"
+          pattern: "${file_out}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@erikrikarddaniel"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/cat/fastq/meta.yml b/modules/nf-core/cat/fastq/meta.yml
index db4ac3c79..91ff2fb5f 100644
--- a/modules/nf-core/cat/fastq/meta.yml
+++ b/modules/nf-core/cat/fastq/meta.yml
@@ -10,30 +10,33 @@ tools:
         The cat utility reads files sequentially, writing them to the standard output.
       documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html
       licence: ["GPL-3.0-or-later"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files to be concatenated.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FastQ files to be concatenated.
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - reads:
-      type: file
-      description: Merged fastq file
-      pattern: "*.{merged.fastq.gz}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.merged.fastq.gz":
+          type: file
+          description: Merged fastq file
+          pattern: "*.{merged.fastq.gz}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml
index 8dfecc184..159404d08 100644
--- a/modules/nf-core/fastp/meta.yml
+++ b/modules/nf-core/fastp/meta.yml
@@ -11,66 +11,100 @@ tools:
       documentation: https://github.com/OpenGene/fastp
       doi: 10.1093/bioinformatics/bty560
       licence: ["MIT"]
+      identifier: biotools:fastp
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads.
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively. If you wish to run interleaved paired-end data,  supply as single-end data
-        but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module.
-  - adapter_fasta:
-      type: file
-      description: File in FASTA format containing possible adapters to remove.
-      pattern: "*.{fasta,fna,fas,fa}"
-  - discard_trimmed_pass:
-      type: boolean
-      description: Specify true to not write any reads that pass trimming thresholds. |
-        This can be used to use fastp for the output report only.
-  - save_trimmed_fail:
-      type: boolean
-      description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz`
-  - save_merged:
-      type: boolean
-      description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz`
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads.
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+          respectively. If you wish to run interleaved paired-end data,  supply as single-end data
+          but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module.
+  - - adapter_fasta:
+        type: file
+        description: File in FASTA format containing possible adapters to remove.
+        pattern: "*.{fasta,fna,fas,fa}"
+  - - discard_trimmed_pass:
+        type: boolean
+        description: Specify true to not write any reads that pass trimming thresholds.
+          | This can be used to use fastp for the output report only.
+  - - save_trimmed_fail:
+        type: boolean
+        description: Specify true to save files that failed to pass trimming thresholds
+          ending in `*.fail.fastq.gz`
+  - - save_merged:
+        type: boolean
+        description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz`
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - reads:
-      type: file
-      description: The trimmed/modified/unmerged fastq reads
-      pattern: "*fastp.fastq.gz"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fastp.fastq.gz":
+          type: file
+          description: The trimmed/modified/unmerged fastq reads
+          pattern: "*fastp.fastq.gz"
   - json:
-      type: file
-      description: Results in JSON format
-      pattern: "*.json"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.json":
+          type: file
+          description: Results in JSON format
+          pattern: "*.json"
   - html:
-      type: file
-      description: Results in HTML format
-      pattern: "*.html"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.html":
+          type: file
+          description: Results in HTML format
+          pattern: "*.html"
   - log:
-      type: file
-      description: fastq log file
-      pattern: "*.log"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.log":
+          type: file
+          description: fastq log file
+          pattern: "*.log"
   - reads_fail:
-      type: file
-      description: Reads the failed the preprocessing
-      pattern: "*fail.fastq.gz"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fail.fastq.gz":
+          type: file
+          description: Reads the failed the preprocessing
+          pattern: "*fail.fastq.gz"
   - reads_merged:
-      type: file
-      description: Reads that were successfully merged
-      pattern: "*.{merged.fastq.gz}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.merged.fastq.gz":
+          type: file
+          description: Reads that were successfully merged
+          pattern: "*.{merged.fastq.gz}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@kevinmenden"
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index ee5507e06..4827da7af 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -16,35 +16,44 @@ tools:
       homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
       documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
       licence: ["GPL-2.0-only"]
+      identifier: biotools:fastqc
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+          respectively.
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - html:
-      type: file
-      description: FastQC report
-      pattern: "*_{fastqc.html}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.html":
+          type: file
+          description: FastQC report
+          pattern: "*_{fastqc.html}"
   - zip:
-      type: file
-      description: FastQC report archive
-      pattern: "*_{fastqc.zip}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.zip":
+          type: file
+          description: FastQC report archive
+          pattern: "*_{fastqc.zip}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@grst"
diff --git a/modules/nf-core/gatk4/bedtointervallist/meta.yml b/modules/nf-core/gatk4/bedtointervallist/meta.yml
index 187da8850..25348e162 100644
--- a/modules/nf-core/gatk4/bedtointervallist/meta.yml
+++ b/modules/nf-core/gatk4/bedtointervallist/meta.yml
@@ -15,34 +15,45 @@ tools:
       documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s
       doi: 10.1158/1538-7445.AM2017-3590
       licence: ["Apache-2.0"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test']
-  - bed:
-      type: file
-      description: Input bed file
-      pattern: "*.bed"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - dict:
-      type: file
-      description: Sequence dictionary
-      pattern: "*.dict"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test']
+    - bed:
+        type: file
+        description: Input bed file
+        pattern: "*.bed"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - dict:
+        type: file
+        description: Sequence dictionary
+        pattern: "*.dict"
 output:
   - interval_list:
-      type: file
-      description: gatk interval list file
-      pattern: "*.interval_list"
+      - meta:
+          type: file
+          description: gatk interval list file
+          pattern: "*.interval_list"
+      - "*.interval_list":
+          type: file
+          description: gatk interval list file
+          pattern: "*.interval_list"
+      - _list:
+          type: file
+          description: gatk interval list file
+          pattern: "*.interval_list"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@kevinmenden"
   - "@ramprasadn"
diff --git a/modules/nf-core/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/gatk4/createsequencedictionary/meta.yml
index f9d70be09..7b5156bb3 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/meta.yml
+++ b/modules/nf-core/gatk4/createsequencedictionary/meta.yml
@@ -15,25 +15,32 @@ tools:
       documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s
       doi: 10.1158/1538-7445.AM2017-3590
       licence: ["Apache-2.0"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - fasta:
-      type: file
-      description: Input fasta file
-      pattern: "*.{fasta,fa}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: Input fasta file
+        pattern: "*.{fasta,fa}"
 output:
   - dict:
-      type: file
-      description: gatk dictionary file
-      pattern: "*.{dict}"
+      - meta:
+          type: file
+          description: gatk dictionary file
+          pattern: "*.{dict}"
+      - "*.dict":
+          type: file
+          description: gatk dictionary file
+          pattern: "*.{dict}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@maxulysse"
   - "@ramprasadn"
diff --git a/modules/nf-core/gatk4/markduplicates/environment.yml b/modules/nf-core/gatk4/markduplicates/environment.yml
index 9fe34eb36..3c73c17e4 100644
--- a/modules/nf-core/gatk4/markduplicates/environment.yml
+++ b/modules/nf-core/gatk4/markduplicates/environment.yml
@@ -1,7 +1,8 @@
 channels:
   - conda-forge
   - bioconda
+
 dependencies:
   - bioconda::gatk4=4.5.0.0
-  - bioconda::samtools=1.19.2
   - bioconda::htslib=1.19.1
+  - bioconda::samtools=1.19.2
diff --git a/modules/nf-core/gatk4/markduplicates/meta.yml b/modules/nf-core/gatk4/markduplicates/meta.yml
index b0f09d4b8..4772c5f39 100644
--- a/modules/nf-core/gatk4/markduplicates/meta.yml
+++ b/modules/nf-core/gatk4/markduplicates/meta.yml
@@ -1,5 +1,6 @@
 name: gatk4_markduplicates
-description: This tool locates and tags duplicate reads in a BAM or SAM file, where duplicate reads are defined as originating from a single fragment of DNA.
+description: This tool locates and tags duplicate reads in a BAM or SAM file, where
+  duplicate reads are defined as originating from a single fragment of DNA.
 keywords:
   - bam
   - gatk4
@@ -7,60 +8,90 @@ keywords:
   - sort
 tools:
   - gatk4:
-      description: Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size.
+      description: Developed in the Data Sciences Platform at the Broad Institute, the
+        toolkit offers a wide variety of tools with a primary focus on variant discovery
+        and genotyping. Its powerful processing engine and high-performance computing
+        features make it capable of taking on projects of any size.
       homepage: https://gatk.broadinstitute.org/hc/en-us
       documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-
       tool_dev_url: https://github.com/broadinstitute/gatk
       doi: 10.1158/1538-7445.AM2017-3590
       licence: ["MIT"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: Sorted BAM file
-      pattern: "*.{bam}"
-  - fasta:
-      type: file
-      description: Fasta file
-      pattern: "*.{fasta}"
-  - fasta_fai:
-      type: file
-      description: Fasta index file
-      pattern: "*.{fai}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: Sorted BAM file
+        pattern: "*.{bam}"
+  - - fasta:
+        type: file
+        description: Fasta file
+        pattern: "*.{fasta}"
+  - - fasta_fai:
+        type: file
+        description: Fasta index file
+        pattern: "*.{fai}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - bam:
-      type: file
-      description: Marked duplicates BAM file
-      pattern: "*.{bam}"
   - cram:
-      type: file
-      description: Marked duplicates CRAM file
-      pattern: "*.{cram}"
-  - bai:
-      type: file
-      description: BAM index file
-      pattern: "*.{bam.bai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*cram":
+          type: file
+          description: Marked duplicates CRAM file
+          pattern: "*.{cram}"
+  - bam:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*bam":
+          type: file
+          description: Marked duplicates BAM file
+          pattern: "*.{bam}"
   - crai:
-      type: file
-      description: CRAM index file
-      pattern: "*.{cram.crai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.crai":
+          type: file
+          description: CRAM index file
+          pattern: "*.{cram.crai}"
+  - bai:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bai":
+          type: file
+          description: BAM index file
+          pattern: "*.{bam.bai}"
   - metrics:
-      type: file
-      description: Duplicate metrics file generated by GATK
-      pattern: "*.{metrics.txt}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.metrics":
+          type: file
+          description: Duplicate metrics file generated by GATK
+          pattern: "*.{metrics.txt}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@ajodeh-juma"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gffread/meta.yml b/modules/nf-core/gffread/meta.yml
index c06028208..bebe7f575 100644
--- a/modules/nf-core/gffread/meta.yml
+++ b/modules/nf-core/gffread/meta.yml
@@ -1,53 +1,73 @@
 name: gffread
-description: Validate, filter, convert and perform various other operations on GFF files
+description: Validate, filter, convert and perform various other operations on GFF
+  files
 keywords:
   - gff
   - conversion
   - validation
 tools:
   - gffread:
-      description: GFF/GTF utility providing format conversions, region filtering, FASTA sequence extraction and more.
+      description: GFF/GTF utility providing format conversions, region filtering, FASTA
+        sequence extraction and more.
       homepage: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread
       documentation: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread
       tool_dev_url: https://github.com/gpertea/gffread
       doi: 10.12688/f1000research.23297.1
       licence: ["MIT"]
+      identifier: biotools:gffread
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing meta data
-        e.g. [ id:'test' ]
-  - gff:
-      type: file
-      description: A reference file in either the GFF3, GFF2 or GTF format.
-      pattern: "*.{gff, gtf}"
-  - fasta:
-      type: file
-      description: A multi-fasta file with the genomic sequences
-      pattern: "*.{fasta,fa,faa,fas,fsa}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing meta data
+          e.g. [ id:'test' ]
+    - gff:
+        type: file
+        description: A reference file in either the GFF3, GFF2 or GTF format.
+        pattern: "*.{gff, gtf}"
+  - - fasta:
+        type: file
+        description: A multi-fasta file with the genomic sequences
+        pattern: "*.{fasta,fa,faa,fas,fsa}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing meta data
-        e.g. [ id:'test' ]
   - gtf:
-      type: file
-      description: GTF file resulting from the conversion of the GFF input file if '-T' argument is present
-      pattern: "*.{gtf}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing meta data
+            e.g. [ id:'test' ]
+      - "*.gtf":
+          type: file
+          description: GTF file resulting from the conversion of the GFF input file if
+            '-T' argument is present
+          pattern: "*.{gtf}"
   - gffread_gff:
-      type: file
-      description: GFF3 file resulting from the conversion of the GFF input file if '-T' argument is absent
-      pattern: "*.gff3"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing meta data
+            e.g. [ id:'test' ]
+      - "*.gff3":
+          type: file
+          description: GFF3 file resulting from the conversion of the GFF input file if
+            '-T' argument is absent
+          pattern: "*.gff3"
   - gffread_fasta:
-      type: file
-      description: Fasta file produced when either of '-w', '-x', '-y' parameters is present
-      pattern: "*.fasta"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing meta data
+            e.g. [ id:'test' ]
+      - "*.fasta":
+          type: file
+          description: Fasta file produced when either of '-w', '-x', '-y' parameters
+            is present
+          pattern: "*.fasta"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@edmundmiller"
 maintainers:
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index 0fe126444..6f5b867b7 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.25
+  - bioconda::multiqc=1.25.1
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index b9ccebdbb..9724d2f34 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.25--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.25--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.25.1--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.25.1--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index 382c08cbc..b16c18792 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,5 +1,6 @@
 name: multiqc
-description: Aggregate results from bioinformatics analyses across many samples into a single report
+description: Aggregate results from bioinformatics analyses across many samples into
+  a single report
 keywords:
   - QC
   - bioinformatics tools
@@ -12,53 +13,59 @@ tools:
       homepage: https://multiqc.info/
       documentation: https://multiqc.info/docs/
       licence: ["GPL-3.0-or-later"]
+      identifier: biotools:multiqc
 input:
-  - multiqc_files:
-      type: file
-      description: |
-        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-  - multiqc_config:
-      type: file
-      description: Optional config yml for MultiQC
-      pattern: "*.{yml,yaml}"
-  - extra_multiqc_config:
-      type: file
-      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
-      pattern: "*.{yml,yaml}"
-  - multiqc_logo:
-      type: file
-      description: Optional logo file for MultiQC
-      pattern: "*.{png}"
-  - replace_names:
-      type: file
-      description: |
-        Optional two-column sample renaming file. First column a set of
-        patterns, second column a set of corresponding replacements. Passed via
-        MultiQC's `--replace-names` option.
-      pattern: "*.{tsv}"
-  - sample_names:
-      type: file
-      description: |
-        Optional TSV file with headers, passed to the MultiQC --sample_names
-        argument.
-      pattern: "*.{tsv}"
+  - - multiqc_files:
+        type: file
+        description: |
+          List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - - multiqc_config:
+        type: file
+        description: Optional config yml for MultiQC
+        pattern: "*.{yml,yaml}"
+  - - extra_multiqc_config:
+        type: file
+        description: Second optional config yml for MultiQC. Will override common sections
+          in multiqc_config.
+        pattern: "*.{yml,yaml}"
+  - - multiqc_logo:
+        type: file
+        description: Optional logo file for MultiQC
+        pattern: "*.{png}"
+  - - replace_names:
+        type: file
+        description: |
+          Optional two-column sample renaming file. First column a set of
+          patterns, second column a set of corresponding replacements. Passed via
+          MultiQC's `--replace-names` option.
+        pattern: "*.{tsv}"
+  - - sample_names:
+        type: file
+        description: |
+          Optional TSV file with headers, passed to the MultiQC --sample_names
+          argument.
+        pattern: "*.{tsv}"
 output:
   - report:
-      type: file
-      description: MultiQC report file
-      pattern: "multiqc_report.html"
+      - "*multiqc_report.html":
+          type: file
+          description: MultiQC report file
+          pattern: "multiqc_report.html"
   - data:
-      type: directory
-      description: MultiQC data dir
-      pattern: "multiqc_data"
+      - "*_data":
+          type: directory
+          description: MultiQC data dir
+          pattern: "multiqc_data"
   - plots:
-      type: file
-      description: Plots created by MultiQC
-      pattern: "*_data"
+      - "*_plots":
+          type: file
+          description: Plots created by MultiQC
+          pattern: "*_data"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@abhi18av"
   - "@bunop"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index b779e4692..2fcbb5ff7 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-10T12:41:34.562023"
+        "timestamp": "2024-10-02T17:51:46.317523"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-10T11:27:11.933869532"
+        "timestamp": "2024-10-02T17:52:20.680978"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-10T11:26:56.709849369"
+        "timestamp": "2024-10-02T17:52:09.185842"
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/meta.yml b/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
index e2749cb62..0947048dc 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
+++ b/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
@@ -13,34 +13,43 @@ tools:
       documentation: "https://broadinstitute.github.io/picard/"
       tool_dev_url: "https://github.com/broadinstitute/picard"
       licence: ["MIT"]
+      identifier: biotools:picard_tools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - pdf:
-      type: file
-      description: Histogram plots of the insert size metrics computed by Picard
-      pattern: "*.pdf"
   - metrics:
-      type: file
-      description: Values used by Picard to generate the insert size histograms
-      pattern: "*.txt"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.txt":
+          type: file
+          description: Values used by Picard to generate the insert size histograms
+          pattern: "*.txt"
+  - histogram:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.pdf":
+          type: file
+          description: Insert size histogram in PDF format
+          pattern: "*.pdf"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@FerriolCalvet"
 maintainers:
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/meta.yml b/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
index 8a7bd2346..15d146baa 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
+++ b/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
@@ -16,45 +16,52 @@ tools:
       documentation: "https://broadinstitute.github.io/picard/"
       tool_dev_url: "https://github.com/broadinstitute/picard"
       licence: ["MIT"]
+      identifier: biotools:picard_tools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false, strandedness:true ]
-  - bam:
-      type: file
-      description: BAM/SAM file
-      pattern: "*.{bam,sam}"
-  - ref_flat:
-      type: file
-      description: Genome ref_flat file
-  - gene_pred:
-      type: file
-      description: genome gene_pred file as an alternative to the ref_flat file
-  - fasta:
-      type: file
-      description: Genome fasta file
-  - rrna_intervals:
-      type: file
-      description: Interval file of ribosomal RNA regions
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false, strandedness:true ]
+    - bam:
+        type: file
+        description: BAM/SAM file
+        pattern: "*.{bam,sam}"
+  - - ref_flat:
+        type: file
+        description: Genome ref_flat file
+  - - fasta:
+        type: file
+        description: Genome fasta file
+  - - rrna_intervals:
+        type: file
+        description: Interval file of ribosomal RNA regions
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - metrics:
-      type: file
-      description: RNA alignment metrics files generated by picard
-      pattern: "*.rna_metrics"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.rna_metrics":
+          type: file
+          description: RNA alignment metrics files generated by picard
+          pattern: "*.rna_metrics"
   - pdf:
-      type: file
-      description: Plot normalized position vs. coverage in a pdf file generated by picard
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.pdf":
+          type: file
+          description: Plot normalized position vs. coverage in a pdf file generated by
+            picard
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@anoronh4"
 maintainers:
diff --git a/modules/nf-core/picard/collectwgsmetrics/meta.yml b/modules/nf-core/picard/collectwgsmetrics/meta.yml
index 5576ef928..bb7480809 100644
--- a/modules/nf-core/picard/collectwgsmetrics/meta.yml
+++ b/modules/nf-core/picard/collectwgsmetrics/meta.yml
@@ -1,5 +1,6 @@
 name: picard_collectwgsmetrics
-description: Collect metrics about coverage and performance of whole genome sequencing (WGS) experiments.
+description: Collect metrics about coverage and performance of whole genome sequencing
+  (WGS) experiments.
 keywords:
   - alignment
   - metrics
@@ -14,55 +15,59 @@ tools:
       homepage: https://broadinstitute.github.io/picard/
       documentation: https://broadinstitute.github.io/picard/
       licence: ["MIT"]
+      identifier: biotools:picard_tools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: Aligned reads file
-      pattern: "*.{bam, cram}"
-  - bai:
-      type: file
-      description: (Optional) Aligned reads file index
-      pattern: "*.{bai,crai}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - fasta:
-      type: file
-      description: Genome fasta file
-      pattern: "*.{fa,fasta,fna}"
-  - meta3:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - fai:
-      type: file
-      description: Genome fasta file index
-      pattern: "*.{fai}"
-  - intervallist:
-      type: file
-      description: Picard Interval List. Defines which contigs to include. Can be generated from a BED file with GATK BedToIntervalList.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: Aligned reads file
+        pattern: "*.{bam, cram}"
+    - bai:
+        type: file
+        description: (Optional) Aligned reads file index
+        pattern: "*.{bai,crai}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: Genome fasta file
+        pattern: "*.{fa,fasta,fna}"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fai:
+        type: file
+        description: Genome fasta file index
+        pattern: "*.{fai}"
+  - - intervallist:
+        type: file
+        description: Picard Interval List. Defines which contigs to include. Can be
+          generated from a BED file with GATK BedToIntervalList.
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - metrics:
-      type: file
-      description: Alignment metrics files generated by picard
-      pattern: "*_{metrics}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*_metrics":
+          type: file
+          description: Alignment metrics files generated by picard
+          pattern: "*_{metrics}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@flowuenne"
diff --git a/modules/nf-core/rrnatranscripts/meta.yml b/modules/nf-core/rrnatranscripts/meta.yml
index 145506011..93f6a10ef 100644
--- a/modules/nf-core/rrnatranscripts/meta.yml
+++ b/modules/nf-core/rrnatranscripts/meta.yml
@@ -10,21 +10,24 @@ tools:
         Extraction of ribosomal RNA
       homepage: https://github.com/nf-core/rnafusion
       licence: ["GPL-3.0-or-later"]
+      identifier: ""
 input:
-  - gtf:
-      type: file
-      description: GTF file
-      pattern: "*.{gtf}"
+  - - gtf:
+        type: file
+        description: GTF file
+        pattern: "*.{gtf}"
 output:
   #
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
   - rrna_gtf:
-      type: file
-      description: GTF file with ribosomal RNA only
-      pattern: "*.{gtf}"
+      - "*rrna_intervals.gtf":
+          type: file
+          description: GTF file with ribosomal RNA only
+          pattern: "*.{gtf}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@rannick"
 maintainers:
diff --git a/modules/nf-core/salmon/index/meta.yml b/modules/nf-core/salmon/index/meta.yml
index fd94dd274..48486a2bb 100644
--- a/modules/nf-core/salmon/index/meta.yml
+++ b/modules/nf-core/salmon/index/meta.yml
@@ -13,22 +13,25 @@ tools:
       manual: https://salmon.readthedocs.io/en/latest/salmon.html
       doi: 10.1038/nmeth.4197
       licence: ["GPL-3.0-or-later"]
+      identifier: biotools:salmon
 input:
-  - genome_fasta:
-      type: file
-      description: Fasta file of the reference genome
-  - transcriptome_fasta:
-      type: file
-      description: Fasta file of the reference transcriptome
+  - - genome_fasta:
+        type: file
+        description: Fasta file of the reference genome
+  - - transcript_fasta:
+        type: file
+        description: Fasta file of the reference transcriptome
 output:
   - index:
-      type: directory
-      description: Folder containing the star index files
-      pattern: "salmon"
+      - salmon:
+          type: directory
+          description: Folder containing the star index files
+          pattern: "salmon"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/salmon/quant/meta.yml b/modules/nf-core/salmon/quant/meta.yml
index a2faf589a..4cacde0f0 100644
--- a/modules/nf-core/salmon/quant/meta.yml
+++ b/modules/nf-core/salmon/quant/meta.yml
@@ -13,47 +13,69 @@ tools:
       manual: https://salmon.readthedocs.io/en/latest/salmon.html
       doi: 10.1038/nmeth.4197
       licence: ["GPL-3.0-or-later"]
+      identifier: biotools:salmon
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files for single-end or paired-end data.
-        Multiple single-end fastqs or pairs of paired-end fastqs are
-        handled.
-  - index:
-      type: directory
-      description: Folder containing the star index files
-  - gtf:
-      type: file
-      description: GTF of the reference transcriptome
-  - transcript_fasta:
-      type: file
-      description: Fasta file of the reference transcriptome
-  - alignment_mode:
-      type: boolean
-      description: whether to run salmon in alignment mode
-  - lib_type:
-      type: string
-      description: |
-        Override library type inferred based on strandedness defined in meta object
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FastQ files for single-end or paired-end data.
+          Multiple single-end fastqs or pairs of paired-end fastqs are
+          handled.
+  - - index:
+        type: directory
+        description: Folder containing the star index files
+  - - gtf:
+        type: file
+        description: GTF of the reference transcriptome
+  - - transcript_fasta:
+        type: file
+        description: Fasta file of the reference transcriptome
+  - - alignment_mode:
+        type: boolean
+        description: whether to run salmon in alignment mode
+  - - lib_type:
+        type: string
+        description: |
+          Override library type inferred based on strandedness defined in meta object
 output:
   - results:
-      type: directory
-      description: Folder containing the quantification results for a specific sample
-      pattern: "${prefix}"
+      - meta:
+          type: directory
+          description: Folder containing the quantification results for a specific sample
+          pattern: "${prefix}"
+      - ${prefix}:
+          type: directory
+          description: Folder containing the quantification results for a specific sample
+          pattern: "${prefix}"
   - json_info:
-      type: file
-      description: File containing meta information from Salmon quant
-      pattern: "*info.json"
+      - meta:
+          type: file
+          description: File containing meta information from Salmon quant
+          pattern: "*info.json"
+      - "*info.json":
+          type: file
+          description: File containing meta information from Salmon quant
+          pattern: "*info.json"
+  - lib_format_counts:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*lib_format_counts.json":
+          type: file
+          description: File containing the library format counts
+          pattern: "*lib_format_counts.json"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml
index b98cbb990..62054fc97 100644
--- a/modules/nf-core/samtools/faidx/environment.yml
+++ b/modules/nf-core/samtools/faidx/environment.yml
@@ -1,7 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
-
 dependencies:
-  - bioconda::htslib=1.20
-  - bioconda::samtools=1.20
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
index bdcdbc954..28c0a81cf 100644
--- a/modules/nf-core/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_FAIDX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
-        'biocontainers/samtools:1.20--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
index f3c25de20..6721b2cb8 100644
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -14,47 +14,62 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fasta:
-      type: file
-      description: FASTA file
-      pattern: "*.{fa,fasta}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fai:
-      type: file
-      description: FASTA index file
-      pattern: "*.{fai}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - fasta:
+        type: file
+        description: FASTA file
+        pattern: "*.{fa,fasta}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - fai:
+        type: file
+        description: FASTA index file
+        pattern: "*.{fai}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - fa:
-      type: file
-      description: FASTA file
-      pattern: "*.{fa}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.{fa,fasta}":
+          type: file
+          description: FASTA file
+          pattern: "*.{fa}"
   - fai:
-      type: file
-      description: FASTA index file
-      pattern: "*.{fai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fai":
+          type: file
+          description: FASTA index file
+          pattern: "*.{fai}"
   - gzi:
-      type: file
-      description: Optional gzip index file for compressed inputs
-      pattern: "*.gzi"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gzi":
+          type: file
+          description: Optional gzip index file for compressed inputs
+          pattern: "*.gzi"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@ewels"
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
index 3223b72bc..1bbb3ec2b 100644
--- a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
@@ -18,7 +18,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
                     
@@ -36,15 +36,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:42:14.779784761"
+        "timestamp": "2024-09-16T07:57:47.450887871"
     },
     "test_samtools_faidx_bgzip": {
         "content": [
@@ -71,7 +71,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
                     
@@ -95,15 +95,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:42:20.256633877"
+        "timestamp": "2024-09-16T07:58:04.804905659"
     },
     "test_samtools_faidx_fasta": {
         "content": [
@@ -124,7 +124,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
                     [
@@ -142,15 +142,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:42:25.632577273"
+        "timestamp": "2024-09-16T07:58:23.831268154"
     },
     "test_samtools_faidx_stub_fasta": {
         "content": [
@@ -171,7 +171,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
                     [
@@ -189,15 +189,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:42:31.058424849"
+        "timestamp": "2024-09-16T07:58:35.600243706"
     },
     "test_samtools_faidx_stub_fai": {
         "content": [
@@ -218,7 +218,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
                     
@@ -236,14 +236,14 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,2db78952923a61e05d50b95518b21856"
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:42:36.479929617"
+        "timestamp": "2024-09-16T07:58:54.705460167"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml
index da2df5e43..62054fc97 100644
--- a/modules/nf-core/samtools/index/environment.yml
+++ b/modules/nf-core/samtools/index/environment.yml
@@ -1,6 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::samtools=1.20
-  - bioconda::htslib=1.20
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index e002585b9..311756102 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_INDEX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
-        'biocontainers/samtools:1.20--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input)
diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml
index 01a4ee03e..db8df0d50 100644
--- a/modules/nf-core/samtools/index/meta.yml
+++ b/modules/nf-core/samtools/index/meta.yml
@@ -15,38 +15,52 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: input file
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - bai:
-      type: file
-      description: BAM/CRAM/SAM index file
-      pattern: "*.{bai,crai,sai}"
-  - crai:
-      type: file
-      description: BAM/CRAM/SAM index file
-      pattern: "*.{bai,crai,sai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bai":
+          type: file
+          description: BAM/CRAM/SAM index file
+          pattern: "*.{bai,crai,sai}"
   - csi:
-      type: file
-      description: CSI index file
-      pattern: "*.{csi}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.csi":
+          type: file
+          description: CSI index file
+          pattern: "*.{csi}"
+  - crai:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.crai":
+          type: file
+          description: BAM/CRAM/SAM index file
+          pattern: "*.{bai,crai,sai}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@ewels"
diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap
index 799d199ce..72d65e81a 100644
--- a/modules/nf-core/samtools/index/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap
@@ -18,7 +18,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ],
                 "bai": [
                     
@@ -36,15 +36,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T16:51:53.9057"
+        "timestamp": "2024-09-16T08:21:25.261127166"
     },
     "crai - stub": {
         "content": [
@@ -65,7 +65,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ],
                 "bai": [
                     
@@ -83,15 +83,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T16:51:45.931558"
+        "timestamp": "2024-09-16T08:21:12.653194876"
     },
     "bai - stub": {
         "content": [
@@ -112,7 +112,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ],
                 "bai": [
                     [
@@ -130,28 +130,28 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T16:51:34.807525"
+        "timestamp": "2024-09-16T08:21:01.854932651"
     },
     "csi": {
         "content": [
             "test.paired_end.sorted.bam.csi",
             [
-                "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T16:52:55.688799"
+        "timestamp": "2024-09-16T08:20:51.485364222"
     },
     "crai": {
         "content": [
@@ -172,7 +172,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ],
                 "bai": [
                     
@@ -190,15 +190,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T16:51:17.609533"
+        "timestamp": "2024-09-16T08:20:40.518873972"
     },
     "bai": {
         "content": [
@@ -219,7 +219,7 @@
                     
                 ],
                 "3": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ],
                 "bai": [
                     [
@@ -237,14 +237,14 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,802c9776d9c5e95314e888cf18e96d77"
+                    "versions.yml:md5,5e09a6fdf76de396728f877193d72315"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T16:51:04.16585"
+        "timestamp": "2024-09-16T08:20:21.184050361"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml
index da2df5e43..62054fc97 100644
--- a/modules/nf-core/samtools/sort/environment.yml
+++ b/modules/nf-core/samtools/sort/environment.yml
@@ -1,6 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::samtools=1.20
-  - bioconda::htslib=1.20
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index 8e019099c..acfd9252c 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_SORT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
-        'biocontainers/samtools:1.20--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta) , path(bam)
@@ -32,7 +32,6 @@ process SAMTOOLS_SORT {
 
     """
     samtools cat \\
-        --threads $task.cpus \\
         ${bam} \\
     | \\
     samtools sort \\
diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml
index 341a7d0eb..a9dbec5a8 100644
--- a/modules/nf-core/samtools/sort/meta.yml
+++ b/modules/nf-core/samtools/sort/meta.yml
@@ -15,52 +15,73 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file(s)
-      pattern: "*.{bam,cram,sam}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'genome' ]
-  - fasta:
-      type: file
-      description: Reference genome FASTA file
-      pattern: "*.{fa,fasta,fna}"
-      optional: true
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file(s)
+        pattern: "*.{bam,cram,sam}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: Reference genome FASTA file
+        pattern: "*.{fa,fasta,fna}"
+        optional: true
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - bam:
-      type: file
-      description: Sorted BAM file
-      pattern: "*.{bam}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bam":
+          type: file
+          description: Sorted BAM file
+          pattern: "*.{bam}"
   - cram:
-      type: file
-      description: Sorted CRAM file
-      pattern: "*.{cram}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.cram":
+          type: file
+          description: Sorted CRAM file
+          pattern: "*.{cram}"
   - crai:
-      type: file
-      description: CRAM index file (optional)
-      pattern: "*.crai"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.crai":
+          type: file
+          description: CRAM index file (optional)
+          pattern: "*.crai"
   - csi:
-      type: file
-      description: BAM index file (optional)
-      pattern: "*.csi"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.csi":
+          type: file
+          description: BAM index file (optional)
+          pattern: "*.csi"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@ewels"
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
index da38d5d15..2d6b29004 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -20,14 +20,14 @@
                 ]
             ],
             [
-                "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T17:19:37.196205"
+        "timestamp": "2024-09-16T08:49:58.207549273"
     },
     "bam - stub": {
         "content": [
@@ -57,7 +57,7 @@
                     ]
                 ],
                 "4": [
-                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
                 ],
                 "bam": [
                     [
@@ -84,15 +84,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T15:54:46.580756"
+        "timestamp": "2024-09-16T08:50:08.630951018"
     },
     "cram - stub": {
         "content": [
@@ -122,7 +122,7 @@
                     
                 ],
                 "4": [
-                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
                 ],
                 "bam": [
                     
@@ -149,15 +149,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T15:57:30.505698"
+        "timestamp": "2024-09-16T08:50:19.061912443"
     },
     "bam": {
         "content": [
@@ -167,7 +167,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.sorted.bam:md5,21c992d59615936b99f2ad008aa54400"
+                    "test.sorted.bam:md5,34aa85e86abefe637f7a4a9887f016fc"
                 ]
             ],
             [
@@ -180,13 +180,13 @@
                 ]
             ],
             [
-                "versions.yml:md5,7a360de20e1d7a6f15a5e8fbe0a9c062"
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T15:54:25.872954"
+        "timestamp": "2024-09-16T08:49:43.971158333"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml
index da2df5e43..62054fc97 100644
--- a/modules/nf-core/samtools/view/environment.yml
+++ b/modules/nf-core/samtools/view/environment.yml
@@ -1,6 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::samtools=1.20
-  - bioconda::htslib=1.20
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
index dc611448c..37e05cec8 100644
--- a/modules/nf-core/samtools/view/main.nf
+++ b/modules/nf-core/samtools/view/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_VIEW {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' :
-        'biocontainers/samtools:1.20--h50ea8bc_0' }"
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
 
     input:
     tuple val(meta), path(input), path(index)
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
index 27be60d08..caa7b0150 100644
--- a/modules/nf-core/samtools/view/meta.yml
+++ b/modules/nf-core/samtools/view/meta.yml
@@ -15,77 +15,120 @@ tools:
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
       licence: ["MIT"]
+      identifier: biotools:samtools
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - input:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - index:
-      type: file
-      description: BAM.BAI/BAM.CSI/CRAM.CRAI file (optional)
-      pattern: "*.{.bai,.csi,.crai}"
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - fasta:
-      type: file
-      description: Reference file the CRAM was created with (optional)
-      pattern: "*.{fasta,fa}"
-  - qname:
-      type: file
-      description: Optional file with read names to output only select alignments
-      pattern: "*.{txt,list}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+    - index:
+        type: file
+        description: BAM.BAI/BAM.CSI/CRAM.CRAI file (optional)
+        pattern: "*.{.bai,.csi,.crai}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - fasta:
+        type: file
+        description: Reference file the CRAM was created with (optional)
+        pattern: "*.{fasta,fa}"
+  - - qname:
+        type: file
+        description: Optional file with read names to output only select alignments
+        pattern: "*.{txt,list}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - bam:
-      type: file
-      description: optional filtered/converted BAM file
-      pattern: "*.{bam}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.bam:
+          type: file
+          description: optional filtered/converted BAM file
+          pattern: "*.{bam}"
   - cram:
-      type: file
-      description: optional filtered/converted CRAM file
-      pattern: "*.{cram}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.cram:
+          type: file
+          description: optional filtered/converted CRAM file
+          pattern: "*.{cram}"
   - sam:
-      type: file
-      description: optional filtered/converted SAM file
-      pattern: "*.{sam}"
-  # bai, csi, and crai are created with `--write-index`
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.sam:
+          type: file
+          description: optional filtered/converted SAM file
+          pattern: "*.{sam}"
   - bai:
-      type: file
-      description: optional BAM file index
-      pattern: "*.{bai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.${file_type}.bai:
+          type: file
+          description: optional BAM file index
+          pattern: "*.{bai}"
   - csi:
-      type: file
-      description: optional tabix BAM file index
-      pattern: "*.{csi}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.${file_type}.csi:
+          type: file
+          description: optional tabix BAM file index
+          pattern: "*.{csi}"
   - crai:
-      type: file
-      description: optional CRAM file index
-      pattern: "*.{crai}"
-  # unselected and unselected_index are created when passing a qname
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.${file_type}.crai:
+          type: file
+          description: optional CRAM file index
+          pattern: "*.{crai}"
   - unselected:
-      type: file
-      description: optional file with unselected alignments
-      pattern: "*.unselected.{bam,cram,sam}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.unselected.${file_type}:
+          type: file
+          description: optional file with unselected alignments
+          pattern: "*.unselected.{bam,cram,sam}"
   - unselected_index:
-      type: file
-      description: index for the "unselected" file
-      pattern: "*.unselected.{bai,csi,crai}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.unselected.${file_type}.{bai,csi,crsi}:
+          type: file
+          description: index for the "unselected" file
+          pattern: "*.unselected.{bai,csi,crai}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@joseespinosa"
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap
index 6bcce9fea..63849b037 100644
--- a/modules/nf-core/samtools/view/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/view/tests/main.nf.test.snap
@@ -56,14 +56,14 @@
     "bam_stub_versions": {
         "content": [
             [
-                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:43:20.390692583"
+        "timestamp": "2024-09-16T09:26:24.461775464"
     },
     "cram_to_bam_index_cram": {
         "content": [
@@ -169,6 +169,16 @@
         },
         "timestamp": "2024-02-12T19:37:56.490286"
     },
+    "cram_to_bam_index_qname_unselected_csi": {
+        "content": [
+            "test.unselected.bam.csi"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.04.3"
+        },
+        "timestamp": "2024-02-12T19:38:23.328458"
+    },
     "bam_csi": {
         "content": [
             [
@@ -208,14 +218,14 @@
     "cram_to_bam_index_qname_versions": {
         "content": [
             [
-                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:43:15.007493874"
+        "timestamp": "2024-09-16T09:25:51.953436682"
     },
     "cram_to_bam_bam": {
         "content": [
@@ -240,14 +250,14 @@
     "cram_to_bam_index_versions": {
         "content": [
             [
-                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:43:09.472376824"
+        "timestamp": "2024-09-16T09:25:14.475388399"
     },
     "cram_to_bam_bai": {
         "content": [
@@ -264,14 +274,14 @@
     "cram_to_bam_versions": {
         "content": [
             [
-                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:43:04.080050906"
+        "timestamp": "2024-09-16T09:24:49.673441798"
     },
     "cram_bam": {
         "content": [
@@ -378,14 +388,14 @@
     "bam_versions": {
         "content": [
             [
-                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-05-28T15:42:52.978954857"
+        "timestamp": "2024-09-16T09:23:27.151650338"
     },
     "cram_to_bam_index_qname_cram": {
         "content": [
@@ -450,14 +460,24 @@
     "cram_versions": {
         "content": [
             [
-                "versions.yml:md5,6cd41a9a3b4a95271ec011ea990a2838"
+                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
             ]
         ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:24:12.95416913"
+    },
+    "cram_to_bam_index_qname_unselected": {
+        "content": [
+            "test.unselected.bam"
+        ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "23.04.3"
         },
-        "timestamp": "2024-05-28T15:42:58.400776109"
+        "timestamp": "2024-02-12T19:38:23.322874"
     },
     "bam_sam": {
         "content": [
diff --git a/modules/nf-core/star/align/environment.yml b/modules/nf-core/star/align/environment.yml
index e3d1c714d..1debc4c9b 100644
--- a/modules/nf-core/star/align/environment.yml
+++ b/modules/nf-core/star/align/environment.yml
@@ -1,8 +1,9 @@
 channels:
   - conda-forge
   - bioconda
+
 dependencies:
-  - bioconda::star=2.7.10a
-  - bioconda::samtools=1.18
   - bioconda::htslib=1.18
+  - bioconda::samtools=1.18
+  - bioconda::star=2.7.10a
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index ae67e0040..b5bc9ddf8 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -21,33 +21,34 @@ process STAR_ALIGN {
     tuple val(meta), path('*Log.progress.out'), emit: log_progress
     path  "versions.yml"                      , emit: versions
 
-    tuple val(meta), path('*d.out.bam')              , optional:true, emit: bam
-    tuple val(meta), path('*sortedByCoord.out.bam')  , optional:true, emit: bam_sorted
-    tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript
-    tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted
-    tuple val(meta), path('*fastq.gz')               , optional:true, emit: fastq
-    tuple val(meta), path('*.tab')                   , optional:true, emit: tab
-    tuple val(meta), path('*.SJ.out.tab')            , optional:true, emit: spl_junc_tab
-    tuple val(meta), path('*.ReadsPerGene.out.tab')  , optional:true, emit: read_per_gene_tab
-    tuple val(meta), path('*.out.junction')          , optional:true, emit: junction
-    tuple val(meta), path('*.out.sam')               , optional:true, emit: sam
-    tuple val(meta), path('*.wig')                   , optional:true, emit: wig
-    tuple val(meta), path('*.bg')                    , optional:true, emit: bedgraph
+    tuple val(meta), path('*d.out.bam')                              , optional:true, emit: bam
+    tuple val(meta), path("${prefix}.sortedByCoord.out.bam")         , optional:true, emit: bam_sorted
+    tuple val(meta), path("${prefix}.Aligned.sortedByCoord.out.bam") , optional:true, emit: bam_sorted_aligned
+    tuple val(meta), path('*toTranscriptome.out.bam')                , optional:true, emit: bam_transcript
+    tuple val(meta), path('*Aligned.unsort.out.bam')                 , optional:true, emit: bam_unsorted
+    tuple val(meta), path('*fastq.gz')                               , optional:true, emit: fastq
+    tuple val(meta), path('*.tab')                                   , optional:true, emit: tab
+    tuple val(meta), path('*.SJ.out.tab')                            , optional:true, emit: spl_junc_tab
+    tuple val(meta), path('*.ReadsPerGene.out.tab')                  , optional:true, emit: read_per_gene_tab
+    tuple val(meta), path('*.out.junction')                          , optional:true, emit: junction
+    tuple val(meta), path('*.out.sam')                               , optional:true, emit: sam
+    tuple val(meta), path('*.wig')                                   , optional:true, emit: wig
+    tuple val(meta), path('*.bg')                                    , optional:true, emit: bedgraph
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     def reads1 = [], reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
     def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""
     def seq_center      = seq_center ? "'CN:$seq_center'" : ""
-    def attrRG          = args.contains("--outSAMattrRGline") ? "" : "--outSAMattrRGline 'ID:$prefix' $seq_center 'SM:$prefix' $seq_platform"
+    attrRG          = args.contains("--outSAMattrRGline") ? "" : "--outSAMattrRGline 'ID:$prefix' $seq_center 'SM:$prefix' $seq_platform"
     def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
-    def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
+    mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
     """
     STAR \\
         --genomeDir $index \\
@@ -79,7 +80,7 @@ process STAR_ALIGN {
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     """
     echo "" | gzip > ${prefix}.unmapped_1.fastq.gz
     echo "" | gzip > ${prefix}.unmapped_2.fastq.gz
diff --git a/modules/nf-core/star/align/meta.yml b/modules/nf-core/star/align/meta.yml
index e80dbb7dd..d9cba2aa7 100644
--- a/modules/nf-core/star/align/meta.yml
+++ b/modules/nf-core/star/align/meta.yml
@@ -14,97 +14,201 @@ tools:
       manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
       doi: 10.1093/bioinformatics/bts635
       licence: ["MIT"]
+      identifier: biotools:star
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively.
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - index:
-      type: directory
-      description: STAR genome index
-      pattern: "star"
-  - meta3:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - gtf:
-      type: file
-      description: Annotation GTF file
-      pattern: "*.{gtf}"
-  - star_ignore_sjdbgtf:
-      type: boolean
-      description: Ignore annotation GTF file
-  - seq_platform:
-      type: string
-      description: Sequencing platform
-  - seq_center:
-      type: string
-      description: Sequencing center
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+          respectively.
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - index:
+        type: directory
+        description: STAR genome index
+        pattern: "star"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - gtf:
+        type: file
+        description: Annotation GTF file
+        pattern: "*.{gtf}"
+  - - star_ignore_sjdbgtf:
+        type: boolean
+        description: Ignore annotation GTF file
+  - - seq_platform:
+        type: string
+        description: Sequencing platform
+  - - seq_center:
+        type: string
+        description: Sequencing center
 output:
-  - bam:
-      type: file
-      description: Output BAM file containing read alignments
-      pattern: "*.{bam}"
   - log_final:
-      type: file
-      description: STAR final log file
-      pattern: "*Log.final.out"
+      - meta:
+          type: file
+          description: STAR final log file
+          pattern: "*Log.final.out"
+      - "*Log.final.out":
+          type: file
+          description: STAR final log file
+          pattern: "*Log.final.out"
   - log_out:
-      type: file
-      description: STAR lot out file
-      pattern: "*Log.out"
+      - meta:
+          type: file
+          description: STAR lot out file
+          pattern: "*Log.out"
+      - "*Log.out":
+          type: file
+          description: STAR lot out file
+          pattern: "*Log.out"
   - log_progress:
-      type: file
-      description: STAR log progress file
-      pattern: "*Log.progress.out"
+      - meta:
+          type: file
+          description: STAR log progress file
+          pattern: "*Log.progress.out"
+      - "*Log.progress.out":
+          type: file
+          description: STAR log progress file
+          pattern: "*Log.progress.out"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+  - bam:
+      - meta:
+          type: file
+          description: Output BAM file containing read alignments
+          pattern: "*.{bam}"
+      - "*d.out.bam":
+          type: file
+          description: Output BAM file containing read alignments
+          pattern: "*.{bam}"
   - bam_sorted:
-      type: file
-      description: Sorted BAM file of read alignments (optional)
-      pattern: "*sortedByCoord.out.bam"
+      - meta:
+          type: file
+          description: Sorted BAM file of read alignments (optional)
+          pattern: "*sortedByCoord.out.bam"
+      - ${prefix}.sortedByCoord.out.bam:
+          type: file
+          description: Sorted BAM file of read alignments (optional)
+          pattern: "*sortedByCoord.out.bam"
+  - bam_sorted_aligned:
+      - meta: {}
+      - ${prefix}.Aligned.sortedByCoord.out.bam: {}
   - bam_transcript:
-      type: file
-      description: Output BAM file of transcriptome alignment (optional)
-      pattern: "*toTranscriptome.out.bam"
+      - meta:
+          type: file
+          description: Output BAM file of transcriptome alignment (optional)
+          pattern: "*toTranscriptome.out.bam"
+      - "*toTranscriptome.out.bam":
+          type: file
+          description: Output BAM file of transcriptome alignment (optional)
+          pattern: "*toTranscriptome.out.bam"
   - bam_unsorted:
-      type: file
-      description: Unsorted BAM file of read alignments (optional)
-      pattern: "*Aligned.unsort.out.bam"
+      - meta:
+          type: file
+          description: Unsorted BAM file of read alignments (optional)
+          pattern: "*Aligned.unsort.out.bam"
+      - "*Aligned.unsort.out.bam":
+          type: file
+          description: Unsorted BAM file of read alignments (optional)
+          pattern: "*Aligned.unsort.out.bam"
   - fastq:
-      type: file
-      description: Unmapped FastQ files (optional)
-      pattern: "*fastq.gz"
+      - meta:
+          type: file
+          description: Unmapped FastQ files (optional)
+          pattern: "*fastq.gz"
+      - "*fastq.gz":
+          type: file
+          description: Unmapped FastQ files (optional)
+          pattern: "*fastq.gz"
   - tab:
-      type: file
-      description: STAR output tab file(s) (optional)
-      pattern: "*.tab"
+      - meta:
+          type: file
+          description: STAR output tab file(s) (optional)
+          pattern: "*.tab"
+      - "*.tab":
+          type: file
+          description: STAR output tab file(s) (optional)
+          pattern: "*.tab"
+  - spl_junc_tab:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*.SJ.out.tab"
+      - "*.SJ.out.tab":
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*.SJ.out.tab"
+  - read_per_gene_tab:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*.ReadsPerGene.out.tab"
+      - "*.ReadsPerGene.out.tab":
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*.ReadsPerGene.out.tab"
   - junction:
-      type: file
-      description: STAR chimeric junction output file (optional)
-      pattern: "*.out.junction"
+      - meta:
+          type: file
+          description: STAR chimeric junction output file (optional)
+          pattern: "*.out.junction"
+      - "*.out.junction":
+          type: file
+          description: STAR chimeric junction output file (optional)
+          pattern: "*.out.junction"
+  - sam:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*.out.sam"
+      - "*.out.sam":
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+          pattern: "*.out.sam"
   - wig:
-      type: file
-      description: STAR output wiggle format file(s) (optional)
-      pattern: "*.wig"
+      - meta:
+          type: file
+          description: STAR output wiggle format file(s) (optional)
+          pattern: "*.wig"
+      - "*.wig":
+          type: file
+          description: STAR output wiggle format file(s) (optional)
+          pattern: "*.wig"
   - bedgraph:
-      type: file
-      description: STAR output bedGraph format file(s) (optional)
-      pattern: "*.bg"
+      - meta:
+          type: file
+          description: STAR output bedGraph format file(s) (optional)
+          pattern: "*.bg"
+      - "*.bg":
+          type: file
+          description: STAR output bedGraph format file(s) (optional)
+          pattern: "*.bg"
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/star/align/tests/main.nf.test.snap b/modules/nf-core/star/align/tests/main.nf.test.snap
index c814eb56c..0da28d37c 100644
--- a/modules/nf-core/star/align/tests/main.nf.test.snap
+++ b/modules/nf-core/star/align/tests/main.nf.test.snap
@@ -26,7 +26,11 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        [
+                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
                     ]
                 ],
                 "11": [
@@ -35,7 +39,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "12": [
@@ -44,7 +48,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "13": [
@@ -53,7 +57,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "14": [
@@ -62,10 +66,19 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "15": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "16": [
                     [
                         {
                             "id": "test",
@@ -105,10 +118,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "6": [
@@ -117,7 +127,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "7": [
@@ -126,7 +136,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "8": [
@@ -135,10 +145,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        [
-                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
+                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "9": [
@@ -148,9 +155,8 @@
                             "single_end": true
                         },
                         [
-                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
@@ -173,10 +179,16 @@
                             "id": "test",
                             "single_end": true
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam_sorted_aligned": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "bam_transcript": [
@@ -312,7 +324,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T15:16:04.712114"
+        "timestamp": "2024-10-01T11:39:15.440397"
     },
     "homo_sapiens - paired_end - arriba - stub": {
         "content": [
@@ -341,7 +353,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        [
+                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
                     ]
                 ],
                 "11": [
@@ -350,7 +366,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "12": [
@@ -359,7 +375,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "13": [
@@ -368,7 +384,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "14": [
@@ -377,10 +393,19 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "15": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "16": [
                     [
                         {
                             "id": "test",
@@ -420,10 +445,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "6": [
@@ -432,7 +454,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "7": [
@@ -441,7 +463,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "8": [
@@ -450,10 +472,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
+                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "9": [
@@ -463,9 +482,8 @@
                             "single_end": false
                         },
                         [
-                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
@@ -488,10 +506,16 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam_sorted_aligned": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "bam_transcript": [
@@ -627,7 +651,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T15:16:28.874293"
+        "timestamp": "2024-10-01T11:40:15.472109"
     },
     "homo_sapiens - single_end": {
         "content": [
@@ -644,13 +668,7 @@
                 ]
             ],
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.Aligned.sortedByCoord.out.bam:md5,c6cfaccaf91bc7fdabed3cfe236d4535"
-                ]
+                
             ],
             [
                 
@@ -711,7 +729,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T18:02:34.35338"
+        "timestamp": "2024-10-01T11:23:08.001162"
     },
     "homo_sapiens - paired_end": {
         "content": [
@@ -728,13 +746,7 @@
                 ]
             ],
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Aligned.sortedByCoord.out.bam:md5,b9ee1c607e07323bc1652ef3babb543f"
-                ]
+                
             ],
             [
                 
@@ -795,7 +807,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T18:03:16.701923"
+        "timestamp": "2024-10-01T11:24:46.295219"
     },
     "homo_sapiens - paired_end - multiple - stub": {
         "content": [
@@ -824,7 +836,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        [
+                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
                     ]
                 ],
                 "11": [
@@ -833,7 +849,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "12": [
@@ -842,7 +858,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "13": [
@@ -851,7 +867,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "14": [
@@ -860,10 +876,19 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "15": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "16": [
                     [
                         {
                             "id": "test",
@@ -903,10 +928,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "6": [
@@ -915,7 +937,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "7": [
@@ -924,7 +946,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "8": [
@@ -933,10 +955,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
+                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "9": [
@@ -946,9 +965,8 @@
                             "single_end": false
                         },
                         [
-                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
@@ -971,10 +989,16 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam_sorted_aligned": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "bam_transcript": [
@@ -1110,7 +1134,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T15:16:51.360287"
+        "timestamp": "2024-10-01T11:41:07.310866"
     },
     "homo_sapiens - paired_end - multiple": {
         "content": [
@@ -1127,13 +1151,7 @@
                 ]
             ],
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Aligned.sortedByCoord.out.bam:md5,ab07c21d63ab0a6c07d171d213c81d5a"
-                ]
+                
             ],
             [
                 
@@ -1194,7 +1212,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T13:13:28.987438"
+        "timestamp": "2024-10-01T11:38:46.605044"
     },
     "homo_sapiens - paired_end - stub": {
         "content": [
@@ -1223,7 +1241,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        [
+                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
                     ]
                 ],
                 "11": [
@@ -1232,7 +1254,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "12": [
@@ -1241,7 +1263,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "13": [
@@ -1250,7 +1272,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "14": [
@@ -1259,10 +1281,19 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "15": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "16": [
                     [
                         {
                             "id": "test",
@@ -1302,10 +1333,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "6": [
@@ -1314,7 +1342,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "7": [
@@ -1323,7 +1351,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "8": [
@@ -1332,10 +1360,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
+                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "9": [
@@ -1345,9 +1370,8 @@
                             "single_end": false
                         },
                         [
-                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
@@ -1370,10 +1394,16 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam_sorted_aligned": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "bam_transcript": [
@@ -1509,7 +1539,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T15:16:16.798018"
+        "timestamp": "2024-10-01T11:39:51.595873"
     },
     "homo_sapiens - paired_end - starfusion": {
         "content": [
@@ -1584,7 +1614,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T13:10:55.371956"
+        "timestamp": "2024-10-01T11:35:43.874508"
     },
     "homo_sapiens - paired_end - arriba": {
         "content": [
@@ -1653,7 +1683,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T13:05:10.7534"
+        "timestamp": "2024-10-01T11:28:32.599223"
     },
     "homo_sapiens - paired_end - starfusion - stub": {
         "content": [
@@ -1682,7 +1712,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        [
+                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
                     ]
                 ],
                 "11": [
@@ -1691,7 +1725,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "12": [
@@ -1700,7 +1734,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "13": [
@@ -1709,7 +1743,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "14": [
@@ -1718,10 +1752,19 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "15": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "16": [
                     [
                         {
                             "id": "test",
@@ -1761,10 +1804,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "6": [
@@ -1773,7 +1813,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "7": [
@@ -1782,7 +1822,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "8": [
@@ -1791,10 +1831,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
+                        "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "9": [
@@ -1804,9 +1841,8 @@
                             "single_end": false
                         },
                         [
-                            "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
@@ -1829,10 +1865,16 @@
                             "id": "test",
                             "single_end": false
                         },
-                        [
-                            "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
+                        "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam_sorted_aligned": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "bam_transcript": [
@@ -1968,6 +2010,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.04.3"
         },
-        "timestamp": "2024-07-22T15:16:40.64399"
+        "timestamp": "2024-10-01T11:40:42.29249"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/star/align/tests/nextflow.arriba.config b/modules/nf-core/star/align/tests/nextflow.arriba.config
index 2324b9e58..cf09323fc 100644
--- a/modules/nf-core/star/align/tests/nextflow.arriba.config
+++ b/modules/nf-core/star/align/tests/nextflow.arriba.config
@@ -9,6 +9,3 @@ process {
     }
 
 }
-
-// Fix chown issue for the output star folder
-docker.runOptions = '--platform=linux/amd64 -u $(id -u):$(id -g)'
diff --git a/modules/nf-core/star/align/tests/nextflow.config b/modules/nf-core/star/align/tests/nextflow.config
index c4ac58088..18bc2ee8a 100644
--- a/modules/nf-core/star/align/tests/nextflow.config
+++ b/modules/nf-core/star/align/tests/nextflow.config
@@ -9,6 +9,3 @@ process {
     }
 
 }
-
-// Fix chown issue for the output star folder
-docker.runOptions = '--platform=linux/amd64 -u $(id -u):$(id -g)'
diff --git a/modules/nf-core/star/align/tests/nextflow.starfusion.config b/modules/nf-core/star/align/tests/nextflow.starfusion.config
index 467b64977..7880bfcf5 100644
--- a/modules/nf-core/star/align/tests/nextflow.starfusion.config
+++ b/modules/nf-core/star/align/tests/nextflow.starfusion.config
@@ -9,6 +9,3 @@ process {
     }
 
 }
-
-// Fix chown issue for the output star folder
-docker.runOptions = '--platform=linux/amd64 -u $(id -u):$(id -g)'
diff --git a/modules/nf-core/star/genomegenerate/environment.yml b/modules/nf-core/star/genomegenerate/environment.yml
index 38611dfa2..1debc4c9b 100644
--- a/modules/nf-core/star/genomegenerate/environment.yml
+++ b/modules/nf-core/star/genomegenerate/environment.yml
@@ -1,8 +1,9 @@
 channels:
   - conda-forge
   - bioconda
+
 dependencies:
-  - bioconda::samtools=1.18
   - bioconda::htslib=1.18
+  - bioconda::samtools=1.18
   - bioconda::star=2.7.10a
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/genomegenerate/meta.yml b/modules/nf-core/star/genomegenerate/meta.yml
index 1061e1b8d..33c1f65f3 100644
--- a/modules/nf-core/star/genomegenerate/meta.yml
+++ b/modules/nf-core/star/genomegenerate/meta.yml
@@ -14,37 +14,40 @@ tools:
       manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf
       doi: 10.1093/bioinformatics/bts635
       licence: ["MIT"]
+      identifier: biotools:star
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fasta:
-      type: file
-      description: Fasta file of the reference genome
-  - meta2:
-      type: map
-      description: |
-        Groovy Map containing reference information
-        e.g. [ id:'test' ]
-  - gtf:
-      type: file
-      description: GTF file of the reference genome
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - fasta:
+        type: file
+        description: Fasta file of the reference genome
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'test' ]
+    - gtf:
+        type: file
+        description: GTF file of the reference genome
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - index:
-      type: directory
-      description: Folder containing the star index files
-      pattern: "star"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - star:
+          type: directory
+          description: Folder containing the star index files
+          pattern: "star"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/stringtie/merge/meta.yml b/modules/nf-core/stringtie/merge/meta.yml
index 5d02d6786..cf6902b32 100644
--- a/modules/nf-core/stringtie/merge/meta.yml
+++ b/modules/nf-core/stringtie/merge/meta.yml
@@ -11,27 +11,29 @@ tools:
       homepage: https://ccb.jhu.edu/software/stringtie/index.shtml
       documentation: https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
       licence: ["MIT"]
+      identifier: biotools:stringtie
 input:
-  - stringtie_gtf:
-      type: file
-      description: |
-        Stringtie transcript gtf output(s).
-      pattern: "*.gtf"
-  - annotation_gtf:
-      type: file
-      description: |
-        Annotation gtf file (optional).
-      pattern: "*.gtf"
+  - - stringtie_gtf:
+        type: file
+        description: |
+          Stringtie transcript gtf output(s).
+        pattern: "*.gtf"
+  - - annotation_gtf:
+        type: file
+        description: |
+          Annotation gtf file (optional).
+        pattern: "*.gtf"
 output:
-  - merged_gtf:
-      type: map
-      description: |
-        Merged gtf from annotation and stringtie output gtfs.
-      pattern: "*.gtf"
+  - gtf:
+      - stringtie.merged.gtf:
+          type: file
+          description: Merged gtf file
+          pattern: "stringtie.merged.gtf"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@yuukiiwa"
 maintainers:
diff --git a/modules/nf-core/stringtie/stringtie/meta.yml b/modules/nf-core/stringtie/stringtie/meta.yml
index d8ebdd88a..e55b2abfc 100644
--- a/modules/nf-core/stringtie/stringtie/meta.yml
+++ b/modules/nf-core/stringtie/stringtie/meta.yml
@@ -12,46 +12,67 @@ tools:
       homepage: https://ccb.jhu.edu/software/stringtie/index.shtml
       documentation: https://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
       licence: ["MIT"]
+      identifier: biotools:stringtie
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: |
-        Stringtie transcript gtf output(s).
-  - annotation_gtf:
-      type: file
-      description: |
-        Annotation gtf file (optional).
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: |
+          Stringtie transcript gtf output(s).
+  - - annotation_gtf:
+        type: file
+        description: |
+          Annotation gtf file (optional).
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - transcript_gtf:
-      type: file
-      description: transcript gtf
-      pattern: "*.{transcripts.gtf}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.transcripts.gtf":
+          type: file
+          description: transcript gtf
+          pattern: "*.{transcripts.gtf}"
+  - abundance:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.abundance.txt":
+          type: file
+          description: abundance
+          pattern: "*.{abundance.txt}"
   - coverage_gtf:
-      type: file
-      description: coverage gtf
-      pattern: "*.{coverage.gtf}"
-  - abudance:
-      type: file
-      description: abudance
-      pattern: "*.{abudance.txt}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.coverage.gtf":
+          type: file
+          description: coverage gtf
+          pattern: "*.{coverage.gtf}"
   - ballgown:
-      type: file
-      description: for running ballgown
-      pattern: "*.{ballgown}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.ballgown":
+          type: file
+          description: for running ballgown
+          pattern: "*.{ballgown}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
 maintainers:
diff --git a/modules/nf-core/ucsc/gtftogenepred/meta.yml b/modules/nf-core/ucsc/gtftogenepred/meta.yml
index 02122e410..cf04154d4 100644
--- a/modules/nf-core/ucsc/gtftogenepred/meta.yml
+++ b/modules/nf-core/ucsc/gtftogenepred/meta.yml
@@ -11,34 +11,43 @@ tools:
       description: Convert GTF files to GenePred format
       homepage: http://hgdownload.cse.ucsc.edu/admin/exe/
       licence: ["varies; see http://genome.ucsc.edu/license"]
+      identifier: ""
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - gtf:
-      type: file
-      description: GTF file
-      pattern: "*.{gtf}"
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - gtf:
+        type: file
+        description: GTF file
+        pattern: "*.{gtf}"
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - genepred:
-      type: file
-      description: genepred file
-      pattern: "*.{genepred}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.genepred":
+          type: file
+          description: genepred file
+          pattern: "*.{genepred}"
   - refflat:
-      type: file
-      description: refflat file
-      pattern: "*.{refflat}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.refflat":
+          type: file
+          description: refflat file
+          pattern: "*.{refflat}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@BarryDigby"
   - "@anoronh4"

From db7cf556e61235a741160711695af3df75e9d530 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Oct 2024 09:46:52 +0200
Subject: [PATCH 050/887] updated changelog

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9a8ad1d49..fe350b46c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,6 +13,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Changed
 
+- Updated modules and migrated non-specific modules to nf-core/modules [#484](https://github.com/nf-core/rnafusion/pull/484)
+
 ### Fixed
 
 - Fixed some Nextflow run-commands in the docs [#491](https://github.com/nf-core/rnafusion/pull/491)

From b8c672a4121f758034f1ca3310294487e5fe8deb Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 8 Oct 2024 12:32:59 +0000
Subject: [PATCH 051/887] Template update for nf-core/tools version 3.0.0

---
 .editorconfig                                 |   4 +
 .github/CONTRIBUTING.md                       |  10 +-
 .github/PULL_REQUEST_TEMPLATE.md              |   2 +-
 .github/workflows/awsfulltest.yml             |  23 +-
 .github/workflows/ci.yml                      |  17 +-
 .github/workflows/download_pipeline.yml       |  53 ++-
 .github/workflows/linting.yml                 |  23 +-
 .github/workflows/linting_comment.yml         |   2 +-
 .github/workflows/release-announcements.yml   |   2 +-
 .../workflows/template_version_comment.yml    |  43 ++
 .gitpod.yml                                   |   7 +-
 .nf-core.yml                                  |  19 +-
 .pre-commit-config.yaml                       |   2 +-
 .prettierignore                               |   1 +
 CITATIONS.md                                  |   4 +-
 README.md                                     |   5 +-
 assets/schema_input.json                      |   2 +-
 conf/base.config                              |  34 +-
 conf/igenomes_ignored.config                  |   9 +
 conf/modules.config                           |   1 -
 conf/test.config                              |  13 +-
 docs/images/mqc_fastqc_adapter.png            | Bin 23458 -> 0 bytes
 docs/images/mqc_fastqc_counts.png             | Bin 33918 -> 0 bytes
 docs/images/mqc_fastqc_quality.png            | Bin 55769 -> 0 bytes
 docs/output.md                                |  11 +-
 docs/usage.md                                 |  12 +-
 main.nf                                       |  10 +-
 modules.json                                  |  12 +-
 modules/nf-core/fastqc/environment.yml        |   2 -
 modules/nf-core/fastqc/main.nf                |   5 +-
 modules/nf-core/fastqc/meta.yml               |  57 +--
 modules/nf-core/fastqc/tests/main.nf.test     | 225 ++++++++---
 .../nf-core/fastqc/tests/main.nf.test.snap    | 370 ++++++++++++++++--
 modules/nf-core/multiqc/environment.yml       |   4 +-
 modules/nf-core/multiqc/main.nf               |  14 +-
 modules/nf-core/multiqc/meta.yml              |  78 ++--
 modules/nf-core/multiqc/tests/main.nf.test    |   8 +
 .../nf-core/multiqc/tests/main.nf.test.snap   |  20 +-
 modules/nf-core/multiqc/tests/nextflow.config |   5 +
 nextflow.config                               | 146 ++++---
 nextflow_schema.json                          |  85 +---
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  56 +--
 .../nf-core/utils_nextflow_pipeline/main.nf   |  24 +-
 .../tests/nextflow.config                     |   2 +-
 .../nf-core/utils_nfcore_pipeline/main.nf     |  45 ++-
 .../nf-core/utils_nfschema_plugin/main.nf     |  46 +++
 .../nf-core/utils_nfschema_plugin/meta.yml    |  35 ++
 .../utils_nfschema_plugin/tests/main.nf.test  | 117 ++++++
 .../tests/nextflow.config                     |   8 +
 .../tests/nextflow_schema.json                |   8 +-
 .../nf-core/utils_nfvalidation_plugin/main.nf |  62 ---
 .../utils_nfvalidation_plugin/meta.yml        |  44 ---
 .../tests/main.nf.test                        | 200 ----------
 .../utils_nfvalidation_plugin/tests/tags.yml  |   2 -
 workflows/rnafusion.nf                        |  23 +-
 55 files changed, 1206 insertions(+), 806 deletions(-)
 create mode 100644 .github/workflows/template_version_comment.yml
 create mode 100644 conf/igenomes_ignored.config
 delete mode 100755 docs/images/mqc_fastqc_adapter.png
 delete mode 100755 docs/images/mqc_fastqc_counts.png
 delete mode 100755 docs/images/mqc_fastqc_quality.png
 create mode 100644 modules/nf-core/multiqc/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/main.nf
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/meta.yml
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
 rename subworkflows/nf-core/{utils_nfvalidation_plugin => utils_nfschema_plugin}/tests/nextflow_schema.json (95%)
 delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
 delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
 delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
 delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml

diff --git a/.editorconfig b/.editorconfig
index 72dda289a..e10588156 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -11,6 +11,7 @@ indent_style = space
 [*.{md,yml,yaml,html,css,scss,js}]
 indent_size = 2
 
+
 # These files are edited and tested upstream in nf-core/modules
 [/modules/nf-core/**]
 charset = unset
@@ -25,9 +26,12 @@ insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
 
+
+
 [/assets/email*]
 indent_size = unset
 
+
 # ignore python and markdown
 [*.{py,md}]
 indent_style = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 8e5d1fefe..e57bc9213 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -19,7 +19,7 @@ If you'd like to write some code for nf-core/rnafusion, the standard workflow is
 1. Check that there isn't already an issue about your idea in the [nf-core/rnafusion issues](https://github.com/nf-core/rnafusion/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this
 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnafusion repository](https://github.com/nf-core/rnafusion) to your GitHub account
 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
-4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
+4. Use `nf-core pipelines schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
 
 If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/).
@@ -40,7 +40,7 @@ There are typically two types of tests that run:
 ### Lint tests
 
 `nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to.
-To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint <pipeline-directory>` command.
+To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core pipelines lint <pipeline-directory>` command.
 
 If any failures or warnings are encountered, please follow the listed URL for more documentation.
 
@@ -75,7 +75,7 @@ If you wish to contribute a new step, please use the following coding standards:
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
 4. Add any new parameters to `nextflow.config` with a default (see below).
-5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool).
+5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool).
 6. Add sanity checks and validation for all relevant parameters.
 7. Perform local tests to validate that the new code works as expected.
 8. If applicable, add a new test command in `.github/workflow/ci.yml`.
@@ -86,7 +86,7 @@ If you wish to contribute a new step, please use the following coding standards:
 
 Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope.
 
-Once there, use `nf-core schema build` to add to `nextflow_schema.json`.
+Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`.
 
 ### Default processes resource requirements
 
@@ -103,7 +103,7 @@ Please use the following naming schemes, to make it easy to understand what is g
 
 ### Nextflow version bumping
 
-If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]`
+If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core pipelines bump-version --nextflow . [min-nf-version]`
 
 ### Images and figures
 
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index ed1d027be..7b8124978 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
 - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md)
 - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
-- [ ] Make sure your code lints (`nf-core lint`).
+- [ ] Make sure your code lints (`nf-core pipelines lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
 - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
 - [ ] Usage Documentation in `docs/usage.md` is updated.
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 3c5e4c8ac..c67770f7a 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -1,18 +1,33 @@
 name: nf-core AWS full size tests
-# This workflow is triggered on published releases.
+# This workflow is triggered on PRs opened against the master branch.
 # It can be additionally triggered manually with GitHub actions workflow dispatch button.
 # It runs the -profile 'test_full' on AWS batch
 
 on:
-  release:
-    types: [published]
+  pull_request:
+    branches:
+      - master
   workflow_dispatch:
+  pull_request_review:
+    types: [submitted]
+
 jobs:
   run-platform:
     name: Run AWS full tests
-    if: github.repository == 'nf-core/rnafusion'
+    if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved'
     runs-on: ubuntu-latest
     steps:
+      - uses: octokit/request-action@v2.x
+        id: check_approvals
+        with:
+          route: GET /repos/${{ github.repository }}/pulls/${{ github.event.review.number }}/reviews
+        env:
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+      - id: test_variables
+        run: |
+          JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}'
+          CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length')
+          test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required
       - name: Launch workflow via Seqera Platform
         uses: seqeralabs/action-tower-launch@v2
         # TODO nf-core: You can customise AWS full pipeline tests as required
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index a776bde84..30fd4427a 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -7,6 +7,7 @@ on:
   pull_request:
   release:
     types: [published]
+  workflow_dispatch:
 
 env:
   NXF_ANSI_LOG: false
@@ -24,7 +25,7 @@ jobs:
     strategy:
       matrix:
         NXF_VER:
-          - "23.04.0"
+          - "24.04.2"
           - "latest-everything"
     steps:
       - name: Check out pipeline code
@@ -38,9 +39,21 @@ jobs:
       - name: Disk space cleanup
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - name: Run pipeline with test data
+      - name: Run pipeline with test data (docker)
         # TODO nf-core: You can customise CI pipeline run tests as required
         # For example: adding multiple test runs with different parameters
         # Remember that you can parallelise this by using strategy.matrix
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
+
+      - name: Run pipeline with test data (singularity)
+        # TODO nf-core: You can customise CI pipeline run tests as required
+        run: |
+          nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results
+        if: "${{ github.base_ref == 'master' }}"
+
+      - name: Run pipeline with test data (conda)
+        # TODO nf-core: You can customise CI pipeline run tests as required
+        run: |
+          nextflow run ${GITHUB_WORKSPACE} -profile test,conda --outdir ./results
+        if: "${{ github.base_ref == 'master' }}"
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 2d20d6442..713dc3e73 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -1,4 +1,4 @@
-name: Test successful pipeline download with 'nf-core download'
+name: Test successful pipeline download with 'nf-core pipelines download'
 
 # Run the workflow when:
 #  - dispatched manually
@@ -8,7 +8,7 @@ on:
   workflow_dispatch:
     inputs:
       testbranch:
-        description: "The specific branch you wish to utilize for the test execution of nf-core download."
+        description: "The specific branch you wish to utilize for the test execution of nf-core pipelines download."
         required: true
         default: "dev"
   pull_request:
@@ -39,9 +39,11 @@ jobs:
         with:
           python-version: "3.12"
           architecture: "x64"
-      - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
+
+      - name: Setup Apptainer
+        uses: eWaterCycle/setup-apptainer@4bb22c52d4f63406c49e94c804632975787312b3 # v2.0.0
         with:
-          singularity-version: 3.8.3
+          apptainer-version: 1.3.4
 
       - name: Install dependencies
         run: |
@@ -54,33 +56,64 @@ jobs:
           echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
           echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV}
 
+      - name: Make a cache directory for the container images
+        run: |
+          mkdir -p ./singularity_container_images
+
       - name: Download the pipeline
         env:
-          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
         run: |
-          nf-core download ${{ env.REPO_LOWERCASE }} \
+          nf-core pipelines download ${{ env.REPO_LOWERCASE }} \
           --revision ${{ env.REPO_BRANCH }} \
           --outdir ./${{ env.REPOTITLE_LOWERCASE }} \
           --compress "none" \
           --container-system 'singularity' \
-          --container-library "quay.io" -l "docker.io" -l "ghcr.io" \
+          --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io" \
           --container-cache-utilisation 'amend' \
-          --download-configuration
+          --download-configuration 'yes'
 
       - name: Inspect download
         run: tree ./${{ env.REPOTITLE_LOWERCASE }}
 
+      - name: Count the downloaded number of container images
+        id: count_initial
+        run: |
+          image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
+          echo "Initial container image count: $image_count"
+          echo "IMAGE_COUNT_INITIAL=$image_count" >> ${GITHUB_ENV}
+
       - name: Run the downloaded pipeline (stub)
         id: stub_run_pipeline
         continue-on-error: true
         env:
-          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
           NXF_SINGULARITY_HOME_MOUNT: true
         run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
       - name: Run the downloaded pipeline (stub run not supported)
         id: run_pipeline
         if: ${{ job.steps.stub_run_pipeline.status == failure() }}
         env:
-          NXF_SINGULARITY_CACHEDIR: ./
+          NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
           NXF_SINGULARITY_HOME_MOUNT: true
         run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
+
+      - name: Count the downloaded number of container images
+        id: count_afterwards
+        run: |
+          image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
+          echo "Post-pipeline run container image count: $image_count"
+          echo "IMAGE_COUNT_AFTER=$image_count" >> ${GITHUB_ENV}
+
+      - name: Compare container image counts
+        run: |
+          if [ "${{ env.IMAGE_COUNT_INITIAL }}" -ne "${{ env.IMAGE_COUNT_AFTER }}" ]; then
+            initial_count=${{ env.IMAGE_COUNT_INITIAL }}
+            final_count=${{ env.IMAGE_COUNT_AFTER }}
+            difference=$((final_count - initial_count))
+            echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!"
+            tree ./singularity_container_images
+            exit 1
+          else
+            echo "The pipeline can be downloaded successfully!"
+          fi
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 1fcafe880..b882838af 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -1,6 +1,6 @@
 name: nf-core linting
 # This workflow is triggered on pushes and PRs to the repository.
-# It runs the `nf-core lint` and markdown lint tests to ensure
+# It runs the `nf-core pipelines lint` and markdown lint tests to ensure
 # that the code meets the nf-core guidelines.
 on:
   push:
@@ -41,17 +41,32 @@ jobs:
           python-version: "3.12"
           architecture: "x64"
 
+      - name: read .nf-core.yml
+        uses: pietrobolcato/action-read-yaml@1.0.0
+        id: read_yml
+        with:
+          config: ${{ github.workspace }}/.nf-core.yaml
+
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install nf-core
+          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
+
+      - name: Run nf-core pipelines lint
+        if: ${{ github.base_ref != 'master' }}
+        env:
+          GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
+        run: nf-core -l lint_log.txt pipelines lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
-      - name: Run nf-core lint
+      - name: Run nf-core pipelines lint --release
+        if: ${{ github.base_ref == 'master' }}
         env:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }}
-        run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
+        run: nf-core -l lint_log.txt pipelines lint --release --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
       - name: Save PR number
         if: ${{ always() }}
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 40acc23f5..42e519bfa 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3
+        uses: dawidd6/action-download-artifact@bf251b5aa9c2f7eeb574a96ee720e24f801b7c11 # v6
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 03ecfcf72..c6ba35df4 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -12,7 +12,7 @@ jobs:
       - name: get topics and convert to hashtags
         id: get_topics
         run: |
-          echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')"  >> $GITHUB_OUTPUT
+          echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" | sed 's/-//g' >> $GITHUB_OUTPUT
 
       - uses: rzr/fediverse-action@master
         with:
diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml
new file mode 100644
index 000000000..9dea41f0d
--- /dev/null
+++ b/.github/workflows/template_version_comment.yml
@@ -0,0 +1,43 @@
+name: nf-core template version comment
+# This workflow is triggered on PRs to check if the pipeline template version matches the latest nf-core version.
+# It posts a comment to the PR, even if it comes from a fork.
+
+on: pull_request_target
+
+jobs:
+  template_version:
+    runs-on: ubuntu-latest
+    steps:
+      - name: Check out pipeline code
+        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+
+      - name: Read template version from .nf-core.yml
+        uses: pietrobolcato/action-read-yaml@1.0.0
+        id: read_yml
+        with:
+          config: ${{ github.workspace }}/.nf-core.yml
+
+      - name: Install nf-core
+        run: |
+          python -m pip install --upgrade pip
+          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
+
+      - name: Check nf-core outdated
+        id: nf_core_outdated
+        run: pip list --outdated | grep nf-core
+
+      - name: Post nf-core template version comment
+        uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
+        if: |
+          ${{ steps.nf_core_outdated.outputs.stdout }} =~ 'nf-core'
+        with:
+          repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }}
+          allow-repeats: false
+          message: |
+            ## :warning: Newer version of the nf-core template is available.
+
+            Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}.
+            Please update your pipeline to the latest version.
+
+            For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync).
+          #
diff --git a/.gitpod.yml b/.gitpod.yml
index 105a1821a..461186376 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -4,17 +4,14 @@ tasks:
     command: |
       pre-commit install --install-hooks
       nextflow self-update
-  - name: unset JAVA_TOOL_OPTIONS
-    command: |
-      unset JAVA_TOOL_OPTIONS
 
 vscode:
   extensions: # based on nf-core.nf-core-extensionpack
-    - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code
+    #- esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code
     - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
     - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
     - mechatroner.rainbow-csv # Highlight columns in csv files in different colors
-    # - nextflow.nextflow                    # Nextflow syntax highlighting
+    - nextflow.nextflow # Nextflow syntax highlighting
     - oderwat.indent-rainbow # Highlight indentation level
     - streetsidesoftware.code-spell-checker # Spelling checker for source code
     - charliermarsh.ruff # Code linter Ruff
diff --git a/.nf-core.yml b/.nf-core.yml
index e0b85a77f..01b1f08ca 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,2 +1,19 @@
+bump_version: null
+lint:
+  files_unchanged:
+    - .github/CONTRIBUTING.md
+    - .github/PULL_REQUEST_TEMPLATE.md
+nf_core_version: 3.0.0
+org_path: null
 repository_type: pipeline
-nf_core_version: "2.14.1"
+template:
+  author: Martin Proks, Annick Renevey
+  description: Nextflow rnafusion analysis pipeline, part of the nf-core community.
+  force: false
+  is_nfcore: true
+  name: rnafusion
+  org: nf-core
+  outdir: .
+  skip_features: null
+  version: 4.0.0dev
+update: null
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 4dc0f1dcd..9e9f0e1c4 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -7,7 +7,7 @@ repos:
           - prettier@3.2.5
 
   - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
-    rev: "2.7.3"
+    rev: "3.0.3"
     hooks:
       - id: editorconfig-checker
         alias: ec
diff --git a/.prettierignore b/.prettierignore
index 437d763d0..610e50692 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -1,3 +1,4 @@
+
 email_template.html
 adaptivecard.json
 slackreport.json
diff --git a/CITATIONS.md b/CITATIONS.md
index 98bdbc607..0fc81801b 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -12,11 +12,11 @@
 
 - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
 
-  > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].
+> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].
 
 - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
 
-  > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
+> Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
 ## Software packaging/containerisation tools
 
diff --git a/README.md b/README.md
index 5075b862d..932243be4 100644
--- a/README.md
+++ b/README.md
@@ -9,7 +9,7 @@
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
@@ -67,8 +67,7 @@ nextflow run nf-core/rnafusion \
 ```
 
 > [!WARNING]
-> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_;
-> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files).
+> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).
 
 For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).
 
diff --git a/assets/schema_input.json b/assets/schema_input.json
index fa630b6c0..4f1f739a0 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -1,5 +1,5 @@
 {
-    "$schema": "http://json-schema.org/draft-07/schema",
+    "$schema": "https://json-schema.org/draft/2020-12/schema",
     "$id": "https://raw.githubusercontent.com/nf-core/rnafusion/master/assets/schema_input.json",
     "title": "nf-core/rnafusion pipeline - params.input schema",
     "description": "Schema for the file provided with params.input",
diff --git a/conf/base.config b/conf/base.config
index 0af2fc0da..f5ec67936 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -11,9 +11,9 @@
 process {
 
     // TODO nf-core: Check the defaults for all processes
-    cpus   = { check_max( 1    * task.attempt, 'cpus'   ) }
-    memory = { check_max( 6.GB * task.attempt, 'memory' ) }
-    time   = { check_max( 4.h  * task.attempt, 'time'   ) }
+    cpus   = { 1      * task.attempt }
+    memory = { 6.GB   * task.attempt }
+    time   = { 4.h    * task.attempt }
 
     errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' }
     maxRetries    = 1
@@ -27,30 +27,30 @@ process {
     // TODO nf-core: Customise requirements for specific processes.
     // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
     withLabel:process_single {
-        cpus   = { check_max( 1                  , 'cpus'    ) }
-        memory = { check_max( 6.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 4.h  * task.attempt, 'time'    ) }
+        cpus   = { 1                   }
+        memory = { 6.GB * task.attempt }
+        time   = { 4.h  * task.attempt }
     }
     withLabel:process_low {
-        cpus   = { check_max( 2     * task.attempt, 'cpus'    ) }
-        memory = { check_max( 12.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 4.h   * task.attempt, 'time'    ) }
+        cpus   = { 2     * task.attempt }
+        memory = { 12.GB * task.attempt }
+        time   = { 4.h   * task.attempt }
     }
     withLabel:process_medium {
-        cpus   = { check_max( 6     * task.attempt, 'cpus'    ) }
-        memory = { check_max( 36.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 8.h   * task.attempt, 'time'    ) }
+        cpus   = { 6     * task.attempt }
+        memory = { 36.GB * task.attempt }
+        time   = { 8.h   * task.attempt }
     }
     withLabel:process_high {
-        cpus   = { check_max( 12    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 72.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 16.h  * task.attempt, 'time'    ) }
+        cpus   = { 12    * task.attempt }
+        memory = { 72.GB * task.attempt }
+        time   = { 16.h  * task.attempt }
     }
     withLabel:process_long {
-        time   = { check_max( 20.h  * task.attempt, 'time'    ) }
+        time   = { 20.h  * task.attempt }
     }
     withLabel:process_high_memory {
-        memory = { check_max( 200.GB * task.attempt, 'memory' ) }
+        memory = { 200.GB * task.attempt }
     }
     withLabel:error_ignore {
         errorStrategy = 'ignore'
diff --git a/conf/igenomes_ignored.config b/conf/igenomes_ignored.config
new file mode 100644
index 000000000..b4034d824
--- /dev/null
+++ b/conf/igenomes_ignored.config
@@ -0,0 +1,9 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for iGenomes paths
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Empty genomes dictionary to use when igenomes is ignored.
+----------------------------------------------------------------------------------------
+*/
+
+params.genomes = [:]
diff --git a/conf/modules.config b/conf/modules.config
index d203d2b6e..d266a387f 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -21,7 +21,6 @@ process {
     withName: FASTQC {
         ext.args = '--quiet'
     }
-
     withName: 'MULTIQC' {
         ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
diff --git a/conf/test.config b/conf/test.config
index d54c7159b..955708618 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -10,15 +10,18 @@
 ----------------------------------------------------------------------------------------
 */
 
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
-    // Limit resources so that this can run on GitHub Actions
-    max_cpus   = 2
-    max_memory = '6.GB'
-    max_time   = '6.h'
-
     // Input data
     // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
     // TODO nf-core: Give any required params for the test so that command line flags are not needed
diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png
deleted file mode 100755
index 361d0e47acfb424dea1f326590d1eb2f6dfa26b5..0000000000000000000000000000000000000000
GIT binary patch
literal 0
HcmV?d00001

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diff --git a/docs/output.md b/docs/output.md
index 77b376b31..4a655ea18 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -14,6 +14,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 - [FastQC](#fastqc) - Raw read QC
 - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+
 - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### FastQC
@@ -29,16 +30,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
 
-![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png)
-
-![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png)
-
-![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png)
-
-:::note
-The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
-:::
-
 ### MultiQC
 
 <details markdown="1">
diff --git a/docs/usage.md b/docs/usage.md
index 612cb410f..01ddc7731 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -85,9 +85,9 @@ The above pipeline run specified with a params file in yaml format:
 nextflow run nf-core/rnafusion -profile docker -params-file params.yaml
 ```
 
-with `params.yaml` containing:
+with:
 
-```yaml
+```yaml title="params.yaml"
 input: './samplesheet.csv'
 outdir: './results/'
 genome: 'GRCh37'
@@ -199,14 +199,6 @@ See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config
 
 If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs).
 
-## Azure Resource Requests
-
-To be used with the `azurebatch` profile by specifying the `-profile azurebatch`.
-We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required.
-
-Note that the choice of VM size depends on your quota and the overall workload during the analysis.
-For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes).
-
 ## Running in the background
 
 Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished.
diff --git a/main.nf b/main.nf
index 914752471..3941f007e 100644
--- a/main.nf
+++ b/main.nf
@@ -9,8 +9,6 @@
 ----------------------------------------------------------------------------------------
 */
 
-nextflow.enable.dsl = 2
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS
@@ -20,7 +18,6 @@ nextflow.enable.dsl = 2
 include { RNAFUSION  } from './workflows/rnafusion'
 include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
-
 include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
 
 /*
@@ -56,10 +53,8 @@ workflow NFCORE_RNAFUSION {
     RNAFUSION (
         samplesheet
     )
-
     emit:
     multiqc_report = RNAFUSION.out.multiqc_report // channel: /path/to/multiqc_report.html
-
 }
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -70,27 +65,24 @@ workflow NFCORE_RNAFUSION {
 workflow {
 
     main:
-
     //
     // SUBWORKFLOW: Run initialisation tasks
     //
     PIPELINE_INITIALISATION (
         params.version,
-        params.help,
         params.validate_params,
         params.monochrome_logs,
         args,
         params.outdir,
         params.input
     )
-
+    
     //
     // WORKFLOW: Run main workflow
     //
     NFCORE_RNAFUSION (
         PIPELINE_INITIALISATION.out.samplesheet
     )
-
     //
     // SUBWORKFLOW: Run completion tasks
     //
diff --git a/modules.json b/modules.json
index c253f7934..68b131a5d 100644
--- a/modules.json
+++ b/modules.json
@@ -7,12 +7,12 @@
                 "nf-core": {
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
                         "installed_by": ["modules"]
                     }
                 }
@@ -21,17 +21,17 @@
                 "nf-core": {
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "git_sha": "d20fb2a9cc3e2835e9d067d1046a63252eb17352",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3",
+                        "git_sha": "2fdce49d30c0254f76bc0f13c55c17455c1251ab",
                         "installed_by": ["subworkflows"]
                     },
-                    "utils_nfvalidation_plugin": {
+                    "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa",
+                        "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
index 1787b38a9..691d4c763 100644
--- a/modules/nf-core/fastqc/environment.yml
+++ b/modules/nf-core/fastqc/environment.yml
@@ -1,7 +1,5 @@
-name: fastqc
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
   - bioconda::fastqc=0.12.1
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index d79f1c862..d8989f481 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -26,7 +26,10 @@ process FASTQC {
     def rename_to = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
 
-    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB')
+    // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
+    // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
+    // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
+    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
 
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index ee5507e06..4827da7af 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -16,35 +16,44 @@ tools:
       homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
       documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/
       licence: ["GPL-2.0-only"]
+      identifier: biotools:fastqc
 input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: |
-        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
-        respectively.
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: |
+          List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+          respectively.
 output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
   - html:
-      type: file
-      description: FastQC report
-      pattern: "*_{fastqc.html}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.html":
+          type: file
+          description: FastQC report
+          pattern: "*_{fastqc.html}"
   - zip:
-      type: file
-      description: FastQC report archive
-      pattern: "*_{fastqc.zip}"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.zip":
+          type: file
+          description: FastQC report archive
+          pattern: "*_{fastqc.zip}"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@grst"
diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test
index 70edae4d9..e9d79a074 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test
+++ b/modules/nf-core/fastqc/tests/main.nf.test
@@ -23,17 +23,14 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
-            // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
-            // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
-
-            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_single") }
+                { assert process.success },
+                // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it.
+                // looks like this: <div id="header_filename">Mon 2 Oct 2023<br/>test.gz</div>
+                // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -54,16 +51,14 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
-            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
-            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
-            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
-            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_paired") }
+                { assert process.success },
+                { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+                { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+                { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+                { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+                { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -83,13 +78,11 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") }
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -109,13 +102,11 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_bam") }
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -138,22 +129,20 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
-            { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
-            { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
-            { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
-            { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
-            { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
-            { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
-            { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
-            { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-            { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_multiple") }
+                { assert process.success },
+                { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" },
+                { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" },
+                { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" },
+                { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" },
+                { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" },
+                { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" },
+                { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" },
+                { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" },
+                { assert path(process.out.html[0][1][0]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][2]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert path(process.out.html[0][1][3]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
@@ -173,21 +162,18 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-
-            { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
-            { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
-            { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
-
-            { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") }
+                { assert process.success },
+                { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" },
+                { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" },
+                { assert path(process.out.html[0][1]).text.contains("<tr><td>File type</td><td>Conventional base calls</td></tr>") },
+                { assert snapshot(process.out.versions).match() }
             )
         }
     }
 
     test("sarscov2 single-end [fastq] - stub") {
 
-        options "-stub"
-
+    options "-stub"
         when {
             process {
                 """
@@ -201,12 +187,123 @@ nextflow_process {
 
         then {
             assertAll (
-            { assert process.success },
-            { assert snapshot(process.out.html.collect { file(it[1]).getName() } +
-                                process.out.zip.collect { file(it[1]).getName() } +
-                                process.out.versions ).match("fastqc_stub") }
+                { assert process.success },
+                { assert snapshot(process.out).match() }
             )
         }
     }
 
+    test("sarscov2 paired-end [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 interleaved [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true)
+                ])
+            """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [bam] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 multiple [fastq] - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 custom_prefix - stub") {
+
+    options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'mysample', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
 }
diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap
index 86f7c3115..d5db3092f 100644
--- a/modules/nf-core/fastqc/tests/main.nf.test.snap
+++ b/modules/nf-core/fastqc/tests/main.nf.test.snap
@@ -1,88 +1,392 @@
 {
-    "fastqc_versions_interleaved": {
+    "sarscov2 custom_prefix": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:40:07.293713"
+        "timestamp": "2024-07-22T11:02:16.374038"
     },
-    "fastqc_stub": {
+    "sarscov2 single-end [fastq] - stub": {
         "content": [
-            [
-                "test.html",
-                "test.zip",
-                "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
-            ]
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:24.993809"
+    },
+    "sarscov2 custom_prefix - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "mysample",
+                            "single_end": true
+                        },
+                        "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:31:01.425198"
+        "timestamp": "2024-07-22T11:03:10.93942"
     },
-    "fastqc_versions_multiple": {
+    "sarscov2 interleaved [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:40:55.797907"
+        "timestamp": "2024-07-22T11:01:42.355718"
     },
-    "fastqc_versions_bam": {
+    "sarscov2 paired-end [bam]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:40:26.795862"
+        "timestamp": "2024-07-22T11:01:53.276274"
     },
-    "fastqc_versions_single": {
+    "sarscov2 multiple [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:39:27.043675"
+        "timestamp": "2024-07-22T11:02:05.527626"
     },
-    "fastqc_versions_paired": {
+    "sarscov2 paired-end [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:31.188871"
+    },
+    "sarscov2 paired-end [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:34.273566"
+    },
+    "sarscov2 multiple [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:39:47.584191"
+        "timestamp": "2024-07-22T11:03:02.304411"
     },
-    "fastqc_versions_custom_prefix": {
+    "sarscov2 single-end [fastq]": {
         "content": [
             [
                 "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:01:19.095607"
+    },
+    "sarscov2 interleaved [fastq] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
+        },
+        "timestamp": "2024-07-22T11:02:44.640184"
+    },
+    "sarscov2 paired-end [bam] - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e1cc25ca8af856014824abd842e93978"
+                ],
+                "zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.3"
         },
-        "timestamp": "2024-01-31T17:41:14.576531"
+        "timestamp": "2024-07-22T11:02:53.550742"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index ca39fb67e..f1cd99b07 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -1,7 +1,5 @@
-name: multiqc
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::multiqc=1.21
+  - bioconda::multiqc=1.24.1
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 47ac352f9..b9ccebdbb 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,14 +3,16 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.21--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.25--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.25--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
     path(multiqc_config)
     path(extra_multiqc_config)
     path(multiqc_logo)
+    path(replace_names)
+    path(sample_names)
 
     output:
     path "*multiqc_report.html", emit: report
@@ -23,16 +25,22 @@ process MULTIQC {
 
     script:
     def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : ''
     def config = multiqc_config ? "--config $multiqc_config" : ''
     def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : ''
-    def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : ''
+    def logo = multiqc_logo ? "--cl-config 'custom_logo: \"${multiqc_logo}\"'" : ''
+    def replace = replace_names ? "--replace-names ${replace_names}" : ''
+    def samples = sample_names ? "--sample-names ${sample_names}" : ''
     """
     multiqc \\
         --force \\
         $args \\
         $config \\
+        $prefix \\
         $extra_config \\
         $logo \\
+        $replace \\
+        $samples \\
         .
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index 45a9bc35e..b16c18792 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -1,5 +1,6 @@
 name: multiqc
-description: Aggregate results from bioinformatics analyses across many samples into a single report
+description: Aggregate results from bioinformatics analyses across many samples into
+  a single report
 keywords:
   - QC
   - bioinformatics tools
@@ -12,40 +13,59 @@ tools:
       homepage: https://multiqc.info/
       documentation: https://multiqc.info/docs/
       licence: ["GPL-3.0-or-later"]
+      identifier: biotools:multiqc
 input:
-  - multiqc_files:
-      type: file
-      description: |
-        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-  - multiqc_config:
-      type: file
-      description: Optional config yml for MultiQC
-      pattern: "*.{yml,yaml}"
-  - extra_multiqc_config:
-      type: file
-      description: Second optional config yml for MultiQC. Will override common sections in multiqc_config.
-      pattern: "*.{yml,yaml}"
-  - multiqc_logo:
-      type: file
-      description: Optional logo file for MultiQC
-      pattern: "*.{png}"
+  - - multiqc_files:
+        type: file
+        description: |
+          List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - - multiqc_config:
+        type: file
+        description: Optional config yml for MultiQC
+        pattern: "*.{yml,yaml}"
+  - - extra_multiqc_config:
+        type: file
+        description: Second optional config yml for MultiQC. Will override common sections
+          in multiqc_config.
+        pattern: "*.{yml,yaml}"
+  - - multiqc_logo:
+        type: file
+        description: Optional logo file for MultiQC
+        pattern: "*.{png}"
+  - - replace_names:
+        type: file
+        description: |
+          Optional two-column sample renaming file. First column a set of
+          patterns, second column a set of corresponding replacements. Passed via
+          MultiQC's `--replace-names` option.
+        pattern: "*.{tsv}"
+  - - sample_names:
+        type: file
+        description: |
+          Optional TSV file with headers, passed to the MultiQC --sample_names
+          argument.
+        pattern: "*.{tsv}"
 output:
   - report:
-      type: file
-      description: MultiQC report file
-      pattern: "multiqc_report.html"
+      - "*multiqc_report.html":
+          type: file
+          description: MultiQC report file
+          pattern: "multiqc_report.html"
   - data:
-      type: directory
-      description: MultiQC data dir
-      pattern: "multiqc_data"
+      - "*_data":
+          type: directory
+          description: MultiQC data dir
+          pattern: "multiqc_data"
   - plots:
-      type: file
-      description: Plots created by MultiQC
-      pattern: "*_data"
+      - "*_plots":
+          type: file
+          description: Plots created by MultiQC
+          pattern: "*_data"
   - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@abhi18av"
   - "@bunop"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test
index f1c4242ef..33316a7dd 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test
+++ b/modules/nf-core/multiqc/tests/main.nf.test
@@ -8,6 +8,8 @@ nextflow_process {
     tag "modules_nfcore"
     tag "multiqc"
 
+    config "./nextflow.config"
+
     test("sarscov2 single-end [fastqc]") {
 
         when {
@@ -17,6 +19,8 @@ nextflow_process {
                 input[1] = []
                 input[2] = []
                 input[3] = []
+                input[4] = []
+                input[5] = []
                 """
             }
         }
@@ -41,6 +45,8 @@ nextflow_process {
                 input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true))
                 input[2] = []
                 input[3] = []
+                input[4] = []
+                input[5] = []
                 """
             }
         }
@@ -66,6 +72,8 @@ nextflow_process {
                 input[1] = []
                 input[2] = []
                 input[3] = []
+                input[4] = []
+                input[5] = []
                 """
             }
         }
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index bfebd8029..b779e4692 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-29T08:48:55.657331"
+        "timestamp": "2024-07-10T12:41:34.562023"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-29T08:49:49.071937"
+        "timestamp": "2024-07-10T11:27:11.933869532"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d"
+                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
             ]
         ],
         "meta": {
             "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nextflow": "24.04.2"
         },
-        "timestamp": "2024-02-29T08:49:25.457567"
+        "timestamp": "2024-07-10T11:26:56.709849369"
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/multiqc/tests/nextflow.config b/modules/nf-core/multiqc/tests/nextflow.config
new file mode 100644
index 000000000..c537a6a3e
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'MULTIQC' {
+        ext.prefix = null
+    }
+}
diff --git a/nextflow.config b/nextflow.config
index 172afb674..12203c3dd 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -16,7 +16,6 @@ params {
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'
     igenomes_ignore            = false
-
     // MultiQC options
     multiqc_config             = null
     multiqc_title              = null
@@ -33,48 +32,26 @@ params {
     monochrome_logs              = false
     hook_url                     = null
     help                         = false
+    help_full                    = false
+    show_hidden                  = false
     version                      = false
     pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/'
-
     // Config options
     config_profile_name        = null
     config_profile_description = null
+
     custom_config_version      = 'master'
     custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
     config_profile_contact     = null
     config_profile_url         = null
-
-    // Max resource options
-    // Defaults only, expecting to be overwritten
-    max_memory                 = '128.GB'
-    max_cpus                   = 16
-    max_time                   = '240.h'
-
     // Schema validation default options
-    validationFailUnrecognisedParams = false
-    validationLenientMode            = false
-    validationSchemaIgnoreParams     = 'genomes,igenomes_base'
-    validationShowHiddenParams       = false
-    validate_params                  = true
-
+    validate_params            = true
+    
 }
 
 // Load base.config by default for all pipelines
 includeConfig 'conf/base.config'
 
-// Load nf-core custom profiles from different Institutions
-try {
-    includeConfig "${params.custom_config_base}/nfcore_custom.config"
-} catch (Exception e) {
-    System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
-}
-
-// Load nf-core/rnafusion custom profiles from different institutions.
-try {
-    includeConfig "${params.custom_config_base}/pipeline/rnafusion.config"
-} catch (Exception e) {
-    System.err.println("WARNING: Could not load nf-core/config/rnafusion profiles: ${params.custom_config_base}/pipeline/rnafusion.config")
-}
 profiles {
     debug {
         dumpHashes              = true
@@ -89,7 +66,7 @@ profiles {
         podman.enabled          = false
         shifter.enabled         = false
         charliecloud.enabled    = false
-        conda.channels          = ['conda-forge', 'bioconda', 'defaults']
+        conda.channels          = ['conda-forge', 'bioconda']
         apptainer.enabled       = false
     }
     mamba {
@@ -178,25 +155,23 @@ profiles {
     test_full { includeConfig 'conf/test_full.config' }
 }
 
-// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile
-// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled
-// Set to your registry if you have a mirror of containers
-apptainer.registry   = 'quay.io'
-docker.registry      = 'quay.io'
-podman.registry      = 'quay.io'
-singularity.registry = 'quay.io'
+// Load nf-core custom profiles from different Institutions
+includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null"
 
-// Nextflow plugins
-plugins {
-    id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet
-}
+// Load nf-core/rnafusion custom profiles from different institutions.
+// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
+// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rnafusion.config" : "/dev/null"
+// Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile
+// Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled
+// Set to your registry if you have a mirror of containers
+apptainer.registry    = 'quay.io'
+docker.registry       = 'quay.io'
+podman.registry       = 'quay.io'
+singularity.registry  = 'quay.io'
+charliecloud.registry = 'quay.io'
 
 // Load igenomes.config if required
-if (!params.igenomes_ignore) {
-    includeConfig 'conf/igenomes.config'
-} else {
-    params.genomes = [:]
-}
+includeConfig !params.igenomes_ignore ? 'conf/igenomes.config' : 'conf/igenomes_ignored.config'
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
 // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container.
 // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable.
@@ -208,8 +183,15 @@ env {
     JULIA_DEPOT_PATH = "/usr/local/share/julia"
 }
 
-// Capture exit codes from upstream processes when piping
-process.shell = ['/bin/bash', '-euo', 'pipefail']
+// Set bash options
+process.shell = """\
+bash
+
+set -e # Exit if a tool returns a non-zero status/exit code
+set -u # Treat unset variables and parameters as an error
+set -o pipefail # Returns the status of the last command to exit with a non-zero status or zero if all successfully execute
+set -C # No clobber - prevent output redirection from overwriting files.
+"""
 
 // Disable process selector warnings by default. Use debug profile to enable warnings.
 nextflow.enable.configProcessNamesValidation = false
@@ -238,43 +220,47 @@ manifest {
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = """Nextflow rnafusion analysis pipeline, part of the nf-core community."""
     mainScript      = 'main.nf'
-    nextflowVersion = '!>=23.04.0'
+    nextflowVersion = '!>=24.04.2'
     version         = '4.0.0dev'
     doi             = ''
 }
 
-// Load modules.config for DSL2 module specific options
-includeConfig 'conf/modules.config'
+// Nextflow plugins
+plugins {
+    id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+}
+
+validation {
+    defaultIgnoreParams = ["genomes"]
+    help {
+        enabled = true
+        command = "nextflow run $manifest.name -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
+        fullParameter = "help_full"
+        showHiddenParameter = "show_hidden"
+        beforeText = """
+-\033[2m----------------------------------------------------\033[0m-
+                                        \033[0;32m,--.\033[0;30m/\033[0;32m,-.\033[0m
+\033[0;34m        ___     __   __   __   ___     \033[0;32m/,-._.--~\'\033[0m
+\033[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \033[0;33m}  {\033[0m
+\033[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \033[0;32m\\`-._,-`-,\033[0m
+                                        \033[0;32m`._,._,\'\033[0m
+\033[0;35m  ${manifest.name} ${manifest.version}\033[0m
+-\033[2m----------------------------------------------------\033[0m-
+"""
+        afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { "  https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""}
+* The nf-core framework
+  https://doi.org/10.1038/s41587-020-0439-x
 
-// Function to ensure that resource requirements don't go beyond
-// a maximum limit
-def check_max(obj, type) {
-    if (type == 'memory') {
-        try {
-            if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
-                return params.max_memory as nextflow.util.MemoryUnit
-            else
-                return obj
-        } catch (all) {
-            println "   ### ERROR ###   Max memory '${params.max_memory}' is not valid! Using default value: $obj"
-            return obj
-        }
-    } else if (type == 'time') {
-        try {
-            if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
-                return params.max_time as nextflow.util.Duration
-            else
-                return obj
-        } catch (all) {
-            println "   ### ERROR ###   Max time '${params.max_time}' is not valid! Using default value: $obj"
-            return obj
-        }
-    } else if (type == 'cpus') {
-        try {
-            return Math.min( obj, params.max_cpus as int )
-        } catch (all) {
-            println "   ### ERROR ###   Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
-            return obj
-        }
+* Software dependencies
+  https://github.com/${manifest.name}/blob/master/CITATIONS.md
+"""
+    }
+    summary {
+        beforeText = validation.help.beforeText
+        afterText = validation.help.afterText
     }
 }
+
+// Load modules.config for DSL2 module specific options
+includeConfig 'conf/modules.config'
+
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 91bbdef8c..021e105a2 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -1,10 +1,10 @@
 {
-    "$schema": "http://json-schema.org/draft-07/schema",
+    "$schema": "https://json-schema.org/draft/2020-12/schema",
     "$id": "https://raw.githubusercontent.com/nf-core/rnafusion/master/nextflow_schema.json",
     "title": "nf-core/rnafusion pipeline parameters",
     "description": "Nextflow rnafusion analysis pipeline, part of the nf-core community.",
     "type": "object",
-    "definitions": {
+    "$defs": {
         "input_output_options": {
             "title": "Input/output options",
             "type": "object",
@@ -71,6 +71,14 @@
                     "fa_icon": "fas fa-ban",
                     "hidden": true,
                     "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`."
+                },
+                "igenomes_base": {
+                    "type": "string",
+                    "format": "directory-path",
+                    "description": "The base path to the igenomes reference files",
+                    "fa_icon": "fas fa-ban",
+                    "hidden": true,
+                    "default": "s3://ngi-igenomes/igenomes/"
                 }
             }
         },
@@ -122,41 +130,6 @@
                 }
             }
         },
-        "max_job_request_options": {
-            "title": "Max job request options",
-            "type": "object",
-            "fa_icon": "fab fa-acquisitions-incorporated",
-            "description": "Set the top limit for requested resources for any single job.",
-            "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.",
-            "properties": {
-                "max_cpus": {
-                    "type": "integer",
-                    "description": "Maximum number of CPUs that can be requested for any single job.",
-                    "default": 16,
-                    "fa_icon": "fas fa-microchip",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`"
-                },
-                "max_memory": {
-                    "type": "string",
-                    "description": "Maximum amount of memory that can be requested for any single job.",
-                    "default": "128.GB",
-                    "fa_icon": "fas fa-memory",
-                    "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`"
-                },
-                "max_time": {
-                    "type": "string",
-                    "description": "Maximum amount of time that can be requested for any single job.",
-                    "default": "240.h",
-                    "fa_icon": "far fa-clock",
-                    "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$",
-                    "hidden": true,
-                    "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`"
-                }
-            }
-        },
         "generic_options": {
             "title": "Generic options",
             "type": "object",
@@ -164,12 +137,6 @@
             "description": "Less common options for the pipeline, typically set in a config file.",
             "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.",
             "properties": {
-                "help": {
-                    "type": "boolean",
-                    "description": "Display help text.",
-                    "fa_icon": "fas fa-question-circle",
-                    "hidden": true
-                },
                 "version": {
                     "type": "boolean",
                     "description": "Display version and exit.",
@@ -245,27 +212,6 @@
                     "fa_icon": "fas fa-check-square",
                     "hidden": true
                 },
-                "validationShowHiddenParams": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-eye-slash",
-                    "description": "Show all params when using `--help`",
-                    "hidden": true,
-                    "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters."
-                },
-                "validationFailUnrecognisedParams": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-check-circle",
-                    "description": "Validation of parameters fails when an unrecognised parameter is found.",
-                    "hidden": true,
-                    "help_text": "By default, when an unrecognised parameter is found, it returns a warinig."
-                },
-                "validationLenientMode": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-check-circle",
-                    "description": "Validation of parameters in lenient more.",
-                    "hidden": true,
-                    "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)."
-                },
                 "pipelines_testdata_base_path": {
                     "type": "string",
                     "fa_icon": "far fa-check-circle",
@@ -278,19 +224,16 @@
     },
     "allOf": [
         {
-            "$ref": "#/definitions/input_output_options"
-        },
-        {
-            "$ref": "#/definitions/reference_genome_options"
+            "$ref": "#/$defs/input_output_options"
         },
         {
-            "$ref": "#/definitions/institutional_config_options"
+            "$ref": "#/$defs/reference_genome_options"
         },
         {
-            "$ref": "#/definitions/max_job_request_options"
+            "$ref": "#/$defs/institutional_config_options"
         },
         {
-            "$ref": "#/definitions/generic_options"
+            "$ref": "#/$defs/generic_options"
         }
     ]
 }
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 8d187230d..10054a02d 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -8,17 +8,14 @@
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin'
-include { paramsSummaryMap          } from 'plugin/nf-validation'
-include { fromSamplesheet           } from 'plugin/nf-validation'
-include { UTILS_NEXTFLOW_PIPELINE   } from '../../nf-core/utils_nextflow_pipeline'
+include { UTILS_NFSCHEMA_PLUGIN     } from '../../nf-core/utils_nfschema_plugin'
+include { paramsSummaryMap          } from 'plugin/nf-schema'
+include { samplesheetToList         } from 'plugin/nf-schema'
 include { completionEmail           } from '../../nf-core/utils_nfcore_pipeline'
 include { completionSummary         } from '../../nf-core/utils_nfcore_pipeline'
-include { dashedLine                } from '../../nf-core/utils_nfcore_pipeline'
-include { nfCoreLogo                } from '../../nf-core/utils_nfcore_pipeline'
 include { imNotification            } from '../../nf-core/utils_nfcore_pipeline'
 include { UTILS_NFCORE_PIPELINE     } from '../../nf-core/utils_nfcore_pipeline'
-include { workflowCitation          } from '../../nf-core/utils_nfcore_pipeline'
+include { UTILS_NEXTFLOW_PIPELINE   } from '../../nf-core/utils_nextflow_pipeline'
 
 /*
 ========================================================================================
@@ -30,7 +27,6 @@ workflow PIPELINE_INITIALISATION {
 
     take:
     version           // boolean: Display version and exit
-    help              // boolean: Display help text
     validate_params   // boolean: Boolean whether to validate parameters against the schema at runtime
     monochrome_logs   // boolean: Do not use coloured log outputs
     nextflow_cli_args //   array: List of positional nextflow CLI args
@@ -51,20 +47,16 @@ workflow PIPELINE_INITIALISATION {
         workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1
     )
 
+    
     //
     // Validate parameters and generate parameter summary to stdout
     //
-    pre_help_text = nfCoreLogo(monochrome_logs)
-    post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs)
-    def String workflow_command = "nextflow run ${workflow.manifest.name} -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
-    UTILS_NFVALIDATION_PLUGIN (
-        help,
-        workflow_command,
-        pre_help_text,
-        post_help_text,
+    UTILS_NFSCHEMA_PLUGIN (
+        workflow,
         validate_params,
-        "nextflow_schema.json"
+        null
     )
+    
 
     //
     // Check config provided to the pipeline
@@ -80,8 +72,9 @@ workflow PIPELINE_INITIALISATION {
     //
     // Create channel from input file provided through params.input
     //
+
     Channel
-        .fromSamplesheet("input")
+        .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json"))
         .map {
             meta, fastq_1, fastq_2 ->
                 if (!fastq_2) {
@@ -91,8 +84,8 @@ workflow PIPELINE_INITIALISATION {
                 }
         }
         .groupTuple()
-        .map {
-            validateInputSamplesheet(it)
+        .map { samplesheet ->
+            validateInputSamplesheet(samplesheet)
         }
         .map {
             meta, fastqs ->
@@ -117,13 +110,13 @@ workflow PIPELINE_COMPLETION {
     email           //  string: email address
     email_on_fail   //  string: email address sent on pipeline failure
     plaintext_email // boolean: Send plain-text email instead of HTML
+    
     outdir          //    path: Path to output directory where results will be published
     monochrome_logs // boolean: Disable ANSI colour codes in log output
     hook_url        //  string: hook URL for notifications
     multiqc_report  //  string: Path to MultiQC report
 
     main:
-
     summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
 
     //
@@ -131,11 +124,18 @@ workflow PIPELINE_COMPLETION {
     //
     workflow.onComplete {
         if (email || email_on_fail) {
-            completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList())
+            completionEmail(
+                summary_params,
+                email,
+                email_on_fail,
+                plaintext_email,
+                outdir,
+                monochrome_logs,
+                multiqc_report.toList()
+            )
         }
 
         completionSummary(monochrome_logs)
-
         if (hook_url) {
             imNotification(summary_params, hook_url)
         }
@@ -165,7 +165,7 @@ def validateInputSamplesheet(input) {
     def (metas, fastqs) = input[1..2]
 
     // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end
-    def endedness_ok = metas.collect{ it.single_end }.unique().size == 1
+    def endedness_ok = metas.collect{ meta -> meta.single_end }.unique().size == 1
     if (!endedness_ok) {
         error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}")
     }
@@ -197,7 +197,6 @@ def genomeExistsError() {
         error(error_string)
     }
 }
-
 //
 // Generate methods description for MultiQC
 //
@@ -239,8 +238,10 @@ def methodsDescriptionText(mqc_methods_yaml) {
         // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
         // Removing ` ` since the manifest.doi is a string and not a proper list
         def temp_doi_ref = ""
-        String[] manifest_doi = meta.manifest_map.doi.tokenize(",")
-        for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: <a href=\'https://doi.org/${doi_ref.replace("https://doi.org/", "").replace(" ", "")}\'>${doi_ref.replace("https://doi.org/", "").replace(" ", "")}</a>), "
+        def manifest_doi = meta.manifest_map.doi.tokenize(",")
+        manifest_doi.each { doi_ref ->
+            temp_doi_ref += "(doi: <a href=\'https://doi.org/${doi_ref.replace("https://doi.org/", "").replace(" ", "")}\'>${doi_ref.replace("https://doi.org/", "").replace(" ", "")}</a>), "
+        }
         meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2)
     } else meta["doi_text"] = ""
     meta["nodoi_text"] = meta.manifest_map.doi ? "" : "<li>If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used. </li>"
@@ -261,3 +262,4 @@ def methodsDescriptionText(mqc_methods_yaml) {
 
     return description_html.toString()
 }
+
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
index ac31f28f6..28e32b200 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -2,10 +2,6 @@
 // Subworkflow with functionality that may be useful for any Nextflow pipeline
 //
 
-import org.yaml.snakeyaml.Yaml
-import groovy.json.JsonOutput
-import nextflow.extension.FilesEx
-
 /*
 ========================================================================================
     SUBWORKFLOW DEFINITION
@@ -58,7 +54,7 @@ workflow UTILS_NEXTFLOW_PIPELINE {
 // Generate version string
 //
 def getWorkflowVersion() {
-    String version_string = ""
+    def version_string = "" as String
     if (workflow.manifest.version) {
         def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
         version_string += "${prefix_v}${workflow.manifest.version}"
@@ -79,10 +75,10 @@ def dumpParametersToJSON(outdir) {
     def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
     def filename   = "params_${timestamp}.json"
     def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
-    def jsonStr    = JsonOutput.toJson(params)
-    temp_pf.text   = JsonOutput.prettyPrint(jsonStr)
+    def jsonStr    = groovy.json.JsonOutput.toJson(params)
+    temp_pf.text   = groovy.json.JsonOutput.prettyPrint(jsonStr)
 
-    FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json")
+    nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json")
     temp_pf.delete()
 }
 
@@ -90,7 +86,7 @@ def dumpParametersToJSON(outdir) {
 // When running with -profile conda, warn if channels have not been set-up appropriately
 //
 def checkCondaChannels() {
-    Yaml parser = new Yaml()
+    def parser = new org.yaml.snakeyaml.Yaml()
     def channels = []
     try {
         def config = parser.load("conda config --show channels".execute().text)
@@ -102,14 +98,16 @@ def checkCondaChannels() {
 
     // Check that all channels are present
     // This channel list is ordered by required channel priority.
-    def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults']
+    def required_channels_in_order = ['conda-forge', 'bioconda']
     def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
 
     // Check that they are in the right order
     def channel_priority_violation = false
-    def n = required_channels_in_order.size()
-    for (int i = 0; i < n - 1; i++) {
-        channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1]))
+
+    required_channels_in_order.eachWithIndex { channel, index ->
+        if (index < required_channels_in_order.size() - 1) {
+            channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index+1]))
+        }
     }
 
     if (channels_missing | channel_priority_violation) {
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
index d0a926bf6..a09572e5b 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config
@@ -3,7 +3,7 @@ manifest {
     author          = """nf-core"""
     homePage        = 'https://127.0.0.1'
     description     = """Dummy pipeline"""
-    nextflowVersion  = '!>=23.04.0'
+    nextflowVersion = '!>=23.04.0'
     version         = '9.9.9'
     doi             = 'https://doi.org/10.5281/zenodo.5070524'
 }
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index 14558c392..cbd8495bb 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -2,9 +2,6 @@
 // Subworkflow with utility functions specific to the nf-core pipeline template
 //
 
-import org.yaml.snakeyaml.Yaml
-import nextflow.extension.FilesEx
-
 /*
 ========================================================================================
     SUBWORKFLOW DEFINITION
@@ -34,7 +31,7 @@ workflow UTILS_NFCORE_PIPELINE {
 //  Warn if a -profile or Nextflow config has not been provided to run the pipeline
 //
 def checkConfigProvided() {
-    valid_config = true
+    def valid_config = true as Boolean
     if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
         log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
             "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
@@ -66,11 +63,13 @@ def checkProfileProvided(nextflow_cli_args) {
 //
 def workflowCitation() {
     def temp_doi_ref = ""
-    String[] manifest_doi = workflow.manifest.doi.tokenize(",")
+    def manifest_doi = workflow.manifest.doi.tokenize(",")
     // Using a loop to handle multiple DOIs
     // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
     // Removing ` ` since the manifest.doi is a string and not a proper list
-    for (String doi_ref: manifest_doi) temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
+    manifest_doi.each { doi_ref ->
+        temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
+    }
     return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
         "* The pipeline\n" +
         temp_doi_ref + "\n" +
@@ -84,7 +83,7 @@ def workflowCitation() {
 // Generate workflow version string
 //
 def getWorkflowVersion() {
-    String version_string = ""
+    def version_string = "" as String
     if (workflow.manifest.version) {
         def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : ''
         version_string += "${prefix_v}${workflow.manifest.version}"
@@ -102,8 +101,8 @@ def getWorkflowVersion() {
 // Get software versions for pipeline
 //
 def processVersionsFromYAML(yaml_file) {
-    Yaml yaml = new Yaml()
-    versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] }
+    def yaml = new org.yaml.snakeyaml.Yaml()
+    def versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] }
     return yaml.dumpAsMap(versions).trim()
 }
 
@@ -124,7 +123,7 @@ def workflowVersionToYAML() {
 def softwareVersionsToYAML(ch_versions) {
     return ch_versions
                 .unique()
-                .map { processVersionsFromYAML(it) }
+                .map { version -> processVersionsFromYAML(version) }
                 .unique()
                 .mix(Channel.of(workflowVersionToYAML()))
 }
@@ -134,19 +133,19 @@ def softwareVersionsToYAML(ch_versions) {
 //
 def paramsSummaryMultiqc(summary_params) {
     def summary_section = ''
-    for (group in summary_params.keySet()) {
+    summary_params.keySet().each { group ->
         def group_params = summary_params.get(group)  // This gets the parameters of that particular group
         if (group_params) {
             summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
             summary_section += "    <dl class=\"dl-horizontal\">\n"
-            for (param in group_params.keySet()) {
+            group_params.keySet().sort().each { param ->
                 summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
             }
             summary_section += "    </dl>\n"
         }
     }
 
-    String yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n"
+    def yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" as String
     yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
     yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
     yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
@@ -161,7 +160,7 @@ def paramsSummaryMultiqc(summary_params) {
 // nf-core logo
 //
 def nfCoreLogo(monochrome_logs=true) {
-    Map colors = logColours(monochrome_logs)
+    def colors = logColours(monochrome_logs) as Map
     String.format(
         """\n
         ${dashedLine(monochrome_logs)}
@@ -180,7 +179,7 @@ def nfCoreLogo(monochrome_logs=true) {
 // Return dashed line
 //
 def dashedLine(monochrome_logs=true) {
-    Map colors = logColours(monochrome_logs)
+    def colors = logColours(monochrome_logs) as Map
     return "-${colors.dim}----------------------------------------------------${colors.reset}-"
 }
 
@@ -188,7 +187,7 @@ def dashedLine(monochrome_logs=true) {
 // ANSII colours used for terminal logging
 //
 def logColours(monochrome_logs=true) {
-    Map colorcodes = [:]
+    def colorcodes = [:] as Map
 
     // Reset / Meta
     colorcodes['reset']      = monochrome_logs ? '' : "\033[0m"
@@ -287,7 +286,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     }
 
     def summary = [:]
-    for (group in summary_params.keySet()) {
+    summary_params.keySet().sort().each { group ->
         summary << summary_params[group]
     }
 
@@ -344,10 +343,10 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     def sendmail_html          = sendmail_template.toString()
 
     // Send the HTML e-mail
-    Map colors = logColours(monochrome_logs)
+    def colors = logColours(monochrome_logs) as Map
     if (email_address) {
         try {
-            if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') }
+            if (plaintext_email) { throw new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') }
             // Try to send HTML e-mail using sendmail
             def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
             sendmail_tf.withWriter { w -> w << sendmail_html }
@@ -364,13 +363,13 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     // Write summary e-mail HTML to a file
     def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
     output_hf.withWriter { w -> w << email_html }
-    FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html");
+    nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html");
     output_hf.delete()
 
     // Write summary e-mail TXT to a file
     def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
     output_tf.withWriter { w -> w << email_txt }
-    FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt");
+    nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt");
     output_tf.delete()
 }
 
@@ -378,7 +377,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
 // Print pipeline summary on completion
 //
 def completionSummary(monochrome_logs=true) {
-    Map colors = logColours(monochrome_logs)
+    def colors = logColours(monochrome_logs) as Map
     if (workflow.success) {
         if (workflow.stats.ignoredCount == 0) {
             log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
@@ -395,7 +394,7 @@ def completionSummary(monochrome_logs=true) {
 //
 def imNotification(summary_params, hook_url) {
     def summary = [:]
-    for (group in summary_params.keySet()) {
+    summary_params.keySet().sort().each { group ->
         summary << summary_params[group]
     }
 
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
new file mode 100644
index 000000000..4994303ea
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
@@ -0,0 +1,46 @@
+//
+// Subworkflow that uses the nf-schema plugin to validate parameters and render the parameter summary
+//
+
+include { paramsSummaryLog   } from 'plugin/nf-schema'
+include { validateParameters } from 'plugin/nf-schema'
+
+workflow UTILS_NFSCHEMA_PLUGIN {
+
+    take:
+    input_workflow      // workflow: the workflow object used by nf-schema to get metadata from the workflow
+    validate_params     // boolean:  validate the parameters
+    parameters_schema   // string:   path to the parameters JSON schema.
+                        //           this has to be the same as the schema given to `validation.parametersSchema`
+                        //           when this input is empty it will automatically use the configured schema or
+                        //           "${projectDir}/nextflow_schema.json" as default. This input should not be empty
+                        //           for meta pipelines
+
+    main:
+
+    //
+    // Print parameter summary to stdout. This will display the parameters
+    // that differ from the default given in the JSON schema
+    //
+    if(parameters_schema) {
+        log.info paramsSummaryLog(input_workflow, parameters_schema:parameters_schema)
+    } else {
+        log.info paramsSummaryLog(input_workflow)
+    }
+
+    //
+    // Validate the parameters using nextflow_schema.json or the schema
+    // given via the validation.parametersSchema configuration option
+    //
+    if(validate_params) {
+        if(parameters_schema) {
+            validateParameters(parameters_schema:parameters_schema)
+        } else {
+            validateParameters()
+        }
+    }
+
+    emit:
+    dummy_emit = true
+}
+
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
new file mode 100644
index 000000000..f7d9f0288
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
@@ -0,0 +1,35 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "utils_nfschema_plugin"
+description: Run nf-schema to validate parameters and create a summary of changed parameters
+keywords:
+  - validation
+  - JSON schema
+  - plugin
+  - parameters
+  - summary
+components: []
+input:
+  - input_workflow:
+      type: object
+      description: |
+        The workflow object of the used pipeline.
+        This object contains meta data used to create the params summary log
+  - validate_params:
+      type: boolean
+      description: Validate the parameters and error if invalid.
+  - parameters_schema:
+      type: string
+      description: |
+        Path to the parameters JSON schema.
+        This has to be the same as the schema given to the `validation.parametersSchema` config
+        option. When this input is empty it will automatically use the configured schema or
+        "${projectDir}/nextflow_schema.json" as default. The schema should not be given in this way
+        for meta pipelines.
+output:
+  - dummy_emit:
+      type: boolean
+      description: Dummy emit to make nf-core subworkflows lint happy
+authors:
+  - "@nvnieuwk"
+maintainers:
+  - "@nvnieuwk"
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
new file mode 100644
index 000000000..842dc432a
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
@@ -0,0 +1,117 @@
+nextflow_workflow {
+
+    name "Test Subworkflow UTILS_NFSCHEMA_PLUGIN"
+    script "../main.nf"
+    workflow "UTILS_NFSCHEMA_PLUGIN"
+
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "subworkflows/utils_nfschema_plugin"
+    tag "plugin/nf-schema"
+
+    config "./nextflow.config"
+
+    test("Should run nothing") {
+
+        when {
+
+            params {
+                test_data   = ''
+            }
+
+            workflow {
+                """
+                validate_params = false
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = ""
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should validate params") {
+
+        when {
+
+            params {
+                test_data   = ''
+                outdir      = 1
+            }
+
+            workflow {
+                """
+                validate_params = true
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = ""
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.failed },
+                { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } }
+            )
+        }
+    }
+
+    test("Should run nothing - custom schema") {
+
+        when {
+
+            params {
+                test_data   = ''
+            }
+
+            workflow {
+                """
+                validate_params = false
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+
+    test("Should validate params - custom schema") {
+
+        when {
+
+            params {
+                test_data   = ''
+                outdir      = 1
+            }
+
+            workflow {
+                """
+                validate_params = true
+                input[0] = workflow
+                input[1] = validate_params
+                input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.failed },
+                { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
new file mode 100644
index 000000000..0907ac58f
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
@@ -0,0 +1,8 @@
+plugins {
+    id "nf-schema@2.1.0"
+}
+
+validation {
+    parametersSchema = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
+    monochromeLogs = true
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json
similarity index 95%
rename from subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
rename to subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json
index 7626c1c93..331e0d2f4 100644
--- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json
@@ -1,10 +1,10 @@
 {
-    "$schema": "http://json-schema.org/draft-07/schema",
+    "$schema": "https://json-schema.org/draft/2020-12/schema",
     "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json",
     "title": ". pipeline parameters",
     "description": "",
     "type": "object",
-    "definitions": {
+    "$defs": {
         "input_output_options": {
             "title": "Input/output options",
             "type": "object",
@@ -87,10 +87,10 @@
     },
     "allOf": [
         {
-            "$ref": "#/definitions/input_output_options"
+            "$ref": "#/$defs/input_output_options"
         },
         {
-            "$ref": "#/definitions/generic_options"
+            "$ref": "#/$defs/generic_options"
         }
     ]
 }
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
deleted file mode 100644
index 2585b65d1..000000000
--- a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf
+++ /dev/null
@@ -1,62 +0,0 @@
-//
-// Subworkflow that uses the nf-validation plugin to render help text and parameter summary
-//
-
-/*
-========================================================================================
-    IMPORT NF-VALIDATION PLUGIN
-========================================================================================
-*/
-
-include { paramsHelp         } from 'plugin/nf-validation'
-include { paramsSummaryLog   } from 'plugin/nf-validation'
-include { validateParameters } from 'plugin/nf-validation'
-
-/*
-========================================================================================
-    SUBWORKFLOW DEFINITION
-========================================================================================
-*/
-
-workflow UTILS_NFVALIDATION_PLUGIN {
-
-    take:
-    print_help       // boolean: print help
-    workflow_command //  string: default commmand used to run pipeline
-    pre_help_text    //  string: string to be printed before help text and summary log
-    post_help_text   //  string: string to be printed after help text and summary log
-    validate_params  // boolean: validate parameters
-    schema_filename  //    path: JSON schema file, null to use default value
-
-    main:
-
-    log.debug "Using schema file: ${schema_filename}"
-
-    // Default values for strings
-    pre_help_text    = pre_help_text    ?: ''
-    post_help_text   = post_help_text   ?: ''
-    workflow_command = workflow_command ?: ''
-
-    //
-    // Print help message if needed
-    //
-    if (print_help) {
-        log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text
-        System.exit(0)
-    }
-
-    //
-    // Print parameter summary to stdout
-    //
-    log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text
-
-    //
-    // Validate parameters relative to the parameter JSON schema
-    //
-    if (validate_params){
-        validateParameters(parameters_schema: schema_filename)
-    }
-
-    emit:
-    dummy_emit = true
-}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
deleted file mode 100644
index 3d4a6b04f..000000000
--- a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml
+++ /dev/null
@@ -1,44 +0,0 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
-name: "UTILS_NFVALIDATION_PLUGIN"
-description: Use nf-validation to initiate and validate a pipeline
-keywords:
-  - utility
-  - pipeline
-  - initialise
-  - validation
-components: []
-input:
-  - print_help:
-      type: boolean
-      description: |
-        Print help message and exit
-  - workflow_command:
-      type: string
-      description: |
-        The command to run the workflow e.g. "nextflow run main.nf"
-  - pre_help_text:
-      type: string
-      description: |
-        Text to print before the help message
-  - post_help_text:
-      type: string
-      description: |
-        Text to print after the help message
-  - validate_params:
-      type: boolean
-      description: |
-        Validate the parameters and error if invalid.
-  - schema_filename:
-      type: string
-      description: |
-        The filename of the schema to validate against.
-output:
-  - dummy_emit:
-      type: boolean
-      description: |
-        Dummy emit to make nf-core subworkflows lint happy
-authors:
-  - "@adamrtalbot"
-maintainers:
-  - "@adamrtalbot"
-  - "@maxulysse"
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
deleted file mode 100644
index 5784a33f2..000000000
--- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test
+++ /dev/null
@@ -1,200 +0,0 @@
-nextflow_workflow {
-
-    name "Test Workflow UTILS_NFVALIDATION_PLUGIN"
-    script "../main.nf"
-    workflow "UTILS_NFVALIDATION_PLUGIN"
-    tag "subworkflows"
-    tag "subworkflows_nfcore"
-    tag "plugin/nf-validation"
-    tag "'plugin/nf-validation'"
-    tag "utils_nfvalidation_plugin"
-    tag "subworkflows/utils_nfvalidation_plugin"
-
-    test("Should run nothing") {
-
-        when {
-
-            params {
-                monochrome_logs = true
-                test_data       = ''
-            }
-
-            workflow {
-                """
-                help             = false
-                workflow_command = null
-                pre_help_text    = null
-                post_help_text   = null
-                validate_params  = false
-                schema_filename  = "$moduleTestDir/nextflow_schema.json"
-
-                input[0] = help
-                input[1] = workflow_command
-                input[2] = pre_help_text
-                input[3] = post_help_text
-                input[4] = validate_params
-                input[5] = schema_filename
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success }
-            )
-        }
-    }
-
-    test("Should run help") {
-
-
-        when {
-
-            params {
-                monochrome_logs = true
-                test_data       = ''
-            }
-            workflow {
-                """
-                help             = true
-                workflow_command = null
-                pre_help_text    = null
-                post_help_text   = null
-                validate_params  = false
-                schema_filename  = "$moduleTestDir/nextflow_schema.json"
-
-                input[0] = help
-                input[1] = workflow_command
-                input[2] = pre_help_text
-                input[3] = post_help_text
-                input[4] = validate_params
-                input[5] = schema_filename
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert workflow.exitStatus == 0 },
-                { assert workflow.stdout.any { it.contains('Input/output options') } },
-                { assert workflow.stdout.any { it.contains('--outdir') } }
-            )
-        }
-    }
-
-    test("Should run help with command") {
-
-        when {
-
-            params {
-                monochrome_logs = true
-                test_data       = ''
-            }
-            workflow {
-                """
-                help             = true
-                workflow_command = "nextflow run noorg/doesntexist"
-                pre_help_text    = null
-                post_help_text   = null
-                validate_params  = false
-                schema_filename  = "$moduleTestDir/nextflow_schema.json"
-
-                input[0] = help
-                input[1] = workflow_command
-                input[2] = pre_help_text
-                input[3] = post_help_text
-                input[4] = validate_params
-                input[5] = schema_filename
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert workflow.exitStatus == 0 },
-                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
-                { assert workflow.stdout.any { it.contains('Input/output options') } },
-                { assert workflow.stdout.any { it.contains('--outdir') } }
-            )
-        }
-    }
-
-    test("Should run help with extra text") {
-
-
-        when {
-
-            params {
-                monochrome_logs = true
-                test_data       = ''
-            }
-            workflow {
-                """
-                help             = true
-                workflow_command = "nextflow run noorg/doesntexist"
-                pre_help_text    = "pre-help-text"
-                post_help_text   = "post-help-text"
-                validate_params  = false
-                schema_filename  = "$moduleTestDir/nextflow_schema.json"
-
-                input[0] = help
-                input[1] = workflow_command
-                input[2] = pre_help_text
-                input[3] = post_help_text
-                input[4] = validate_params
-                input[5] = schema_filename
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert workflow.exitStatus == 0 },
-                { assert workflow.stdout.any { it.contains('pre-help-text') } },
-                { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } },
-                { assert workflow.stdout.any { it.contains('Input/output options') } },
-                { assert workflow.stdout.any { it.contains('--outdir') } },
-                { assert workflow.stdout.any { it.contains('post-help-text') } }
-            )
-        }
-    }
-
-    test("Should validate params") {
-
-        when {
-
-            params {
-                monochrome_logs = true
-                test_data       = ''
-                outdir          = 1
-            }
-            workflow {
-                """
-                help             = false
-                workflow_command = null
-                pre_help_text    = null
-                post_help_text   = null
-                validate_params  = true
-                schema_filename  = "$moduleTestDir/nextflow_schema.json"
-
-                input[0] = help
-                input[1] = workflow_command
-                input[2] = pre_help_text
-                input[3] = post_help_text
-                input[4] = validate_params
-                input[5] = schema_filename
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.failed },
-                { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } }
-            )
-        }
-    }
-}
diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
deleted file mode 100644
index 60b1cfff4..000000000
--- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-subworkflows/utils_nfvalidation_plugin:
-  - subworkflows/nf-core/utils_nfvalidation_plugin/**
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2b01b6da0..8192c8229 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -3,10 +3,9 @@
     IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
-
 include { FASTQC                 } from '../modules/nf-core/fastqc/main'
 include { MULTIQC                } from '../modules/nf-core/multiqc/main'
-include { paramsSummaryMap       } from 'plugin/nf-validation'
+include { paramsSummaryMap       } from 'plugin/nf-schema'
 include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
@@ -21,12 +20,10 @@ workflow RNAFUSION {
 
     take:
     ch_samplesheet // channel: samplesheet read in from --input
-
     main:
 
     ch_versions = Channel.empty()
     ch_multiqc_files = Channel.empty()
-
     //
     // MODULE: Run FastQC
     //
@@ -42,11 +39,12 @@ workflow RNAFUSION {
     softwareVersionsToYAML(ch_versions)
         .collectFile(
             storeDir: "${params.outdir}/pipeline_info",
-            name: 'nf_core_pipeline_software_mqc_versions.yml',
+            name: 'nf_core_'  + 'pipeline_software_' +  'mqc_'  + 'versions.yml',
             sort: true,
             newLine: true
         ).set { ch_collated_versions }
 
+
     //
     // MODULE: MultiQC
     //
@@ -59,18 +57,19 @@ workflow RNAFUSION {
         Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
         Channel.empty()
 
+    
     summary_params      = paramsSummaryMap(
         workflow, parameters_schema: "nextflow_schema.json")
     ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
-
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    
     ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
         file(params.multiqc_methods_description, checkIfExists: true) :
         file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
     ch_methods_description                = Channel.value(
         methodsDescriptionText(ch_multiqc_custom_methods_description))
 
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
     ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
     ch_multiqc_files = ch_multiqc_files.mix(
         ch_methods_description.collectFile(
@@ -83,12 +82,14 @@ workflow RNAFUSION {
         ch_multiqc_files.collect(),
         ch_multiqc_config.toList(),
         ch_multiqc_custom_config.toList(),
-        ch_multiqc_logo.toList()
+        ch_multiqc_logo.toList(),
+        [],
+        []
     )
 
-    emit:
-    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+    emit:multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
     versions       = ch_versions                 // channel: [ path(versions.yml) ]
+
 }
 
 /*

From 3dcb0585c01fa7142a46a353ddcc9ffb90223d01 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Wed, 9 Oct 2024 11:07:31 +0000
Subject: [PATCH 052/887] Template update for nf-core/tools version 3.0.1

---
 .editorconfig                                 |   4 -
 .github/CONTRIBUTING.md                       |   2 +-
 .github/workflows/awsfulltest.yml             |   6 +-
 .github/workflows/linting.yml                 |   4 +-
 .nf-core.yml                                  |   2 +-
 .prettierignore                               |   1 -
 docs/output.md                                |   1 -
 modules.json                                  |   6 +-
 modules/nf-core/multiqc/environment.yml       |   2 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  26 +-
 nextflow.config                               |   8 +-
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  12 +-
 .../nf-core/utils_nextflow_pipeline/main.nf   |  46 ++-
 .../nf-core/utils_nfcore_pipeline/main.nf     | 279 ++++++++++--------
 15 files changed, 209 insertions(+), 194 deletions(-)

diff --git a/.editorconfig b/.editorconfig
index e10588156..72dda289a 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -11,7 +11,6 @@ indent_style = space
 [*.{md,yml,yaml,html,css,scss,js}]
 indent_size = 2
 
-
 # These files are edited and tested upstream in nf-core/modules
 [/modules/nf-core/**]
 charset = unset
@@ -26,12 +25,9 @@ insert_final_newline = unset
 trim_trailing_whitespace = unset
 indent_style = unset
 
-
-
 [/assets/email*]
 indent_size = unset
 
-
 # ignore python and markdown
 [*.{py,md}]
 indent_style = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index e57bc9213..7d143af54 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -90,7 +90,7 @@ Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json
 
 ### Default processes resource requirements
 
-Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
+Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
 
 The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block.
 
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index c67770f7a..68975fb0d 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -14,16 +14,18 @@ on:
 jobs:
   run-platform:
     name: Run AWS full tests
-    if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved'
+    # run only if the PR is approved by at least 2 reviewers and against the master branch or manually triggered
+    if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch'
     runs-on: ubuntu-latest
     steps:
       - uses: octokit/request-action@v2.x
         id: check_approvals
         with:
-          route: GET /repos/${{ github.repository }}/pulls/${{ github.event.review.number }}/reviews
+          route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews
         env:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
       - id: test_variables
+        if: github.event_name != 'workflow_dispatch'
         run: |
           JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}'
           CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length')
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index b882838af..a502573c5 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -42,10 +42,10 @@ jobs:
           architecture: "x64"
 
       - name: read .nf-core.yml
-        uses: pietrobolcato/action-read-yaml@1.0.0
+        uses: pietrobolcato/action-read-yaml@1.1.0
         id: read_yml
         with:
-          config: ${{ github.workspace }}/.nf-core.yaml
+          config: ${{ github.workspace }}/.nf-core.yml
 
       - name: Install dependencies
         run: |
diff --git a/.nf-core.yml b/.nf-core.yml
index 01b1f08ca..d7c8a21e4 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -3,7 +3,7 @@ lint:
   files_unchanged:
     - .github/CONTRIBUTING.md
     - .github/PULL_REQUEST_TEMPLATE.md
-nf_core_version: 3.0.0
+nf_core_version: 3.0.1
 org_path: null
 repository_type: pipeline
 template:
diff --git a/.prettierignore b/.prettierignore
index 610e50692..437d763d0 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -1,4 +1,3 @@
-
 email_template.html
 adaptivecard.json
 slackreport.json
diff --git a/docs/output.md b/docs/output.md
index 4a655ea18..501e19501 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -14,7 +14,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 - [FastQC](#fastqc) - Raw read QC
 - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
-
 - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### FastQC
diff --git a/modules.json b/modules.json
index 68b131a5d..243717102 100644
--- a/modules.json
+++ b/modules.json
@@ -12,7 +12,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "b8d36829fa84b6e404364abff787e8b07f6d058c",
                         "installed_by": ["modules"]
                     }
                 }
@@ -21,12 +21,12 @@
                 "nf-core": {
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "d20fb2a9cc3e2835e9d067d1046a63252eb17352",
+                        "git_sha": "9d05360da397692321d377b6102d2fb22507c6ef",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "2fdce49d30c0254f76bc0f13c55c17455c1251ab",
+                        "git_sha": "772684d9d66f37b650c8ba5146ac1ee3ecba2acb",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index f1cd99b07..6f5b867b7 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.24.1
+  - bioconda::multiqc=1.25.1
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index b9ccebdbb..9724d2f34 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.25--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.25--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.25.1--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.25.1--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index b779e4692..2fcbb5ff7 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-10T12:41:34.562023"
+        "timestamp": "2024-10-02T17:51:46.317523"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-10T11:27:11.933869532"
+        "timestamp": "2024-10-02T17:52:20.680978"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-10T11:26:56.709849369"
+        "timestamp": "2024-10-02T17:52:09.185842"
     }
-}
+}
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 12203c3dd..d6f438520 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -12,10 +12,12 @@ params {
     // TODO nf-core: Specify your pipeline's command line flags
     // Input options
     input                      = null
+
     // References
     genome                     = null
     igenomes_base              = 's3://ngi-igenomes/igenomes/'
     igenomes_ignore            = false
+
     // MultiQC options
     multiqc_config             = null
     multiqc_title              = null
@@ -36,6 +38,7 @@ params {
     show_hidden                  = false
     version                      = false
     pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/'
+
     // Config options
     config_profile_name        = null
     config_profile_description = null
@@ -44,9 +47,9 @@ params {
     custom_config_base         = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}"
     config_profile_contact     = null
     config_profile_url         = null
+
     // Schema validation default options
     validate_params            = true
-    
 }
 
 // Load base.config by default for all pipelines
@@ -161,6 +164,7 @@ includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${pa
 // Load nf-core/rnafusion custom profiles from different institutions.
 // TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
 // includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rnafusion.config" : "/dev/null"
+
 // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile
 // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled
 // Set to your registry if you have a mirror of containers
@@ -172,6 +176,7 @@ charliecloud.registry = 'quay.io'
 
 // Load igenomes.config if required
 includeConfig !params.igenomes_ignore ? 'conf/igenomes.config' : 'conf/igenomes_ignored.config'
+
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
 // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container.
 // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable.
@@ -263,4 +268,3 @@ validation {
 
 // Load modules.config for DSL2 module specific options
 includeConfig 'conf/modules.config'
-
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 10054a02d..71a09c15b 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -18,9 +18,9 @@ include { UTILS_NFCORE_PIPELINE     } from '../../nf-core/utils_nfcore_pipeline'
 include { UTILS_NEXTFLOW_PIPELINE   } from '../../nf-core/utils_nextflow_pipeline'
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     SUBWORKFLOW TO INITIALISE PIPELINE
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 workflow PIPELINE_INITIALISATION {
@@ -99,9 +99,9 @@ workflow PIPELINE_INITIALISATION {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     SUBWORKFLOW FOR PIPELINE COMPLETION
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 workflow PIPELINE_COMPLETION {
@@ -147,9 +147,9 @@ workflow PIPELINE_COMPLETION {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     FUNCTIONS
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 //
 // Check and validate pipeline parameters
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
index 28e32b200..2b0dc67a6 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -3,13 +3,12 @@
 //
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     SUBWORKFLOW DEFINITION
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 workflow UTILS_NEXTFLOW_PIPELINE {
-
     take:
     print_version        // boolean: print version
     dump_parameters      // boolean: dump parameters
@@ -22,7 +21,7 @@ workflow UTILS_NEXTFLOW_PIPELINE {
     // Print workflow version and exit on --version
     //
     if (print_version) {
-        log.info "${workflow.manifest.name} ${getWorkflowVersion()}"
+        log.info("${workflow.manifest.name} ${getWorkflowVersion()}")
         System.exit(0)
     }
 
@@ -45,9 +44,9 @@ workflow UTILS_NEXTFLOW_PIPELINE {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     FUNCTIONS
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 //
@@ -72,11 +71,11 @@ def getWorkflowVersion() {
 // Dump pipeline parameters to a JSON file
 //
 def dumpParametersToJSON(outdir) {
-    def timestamp  = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-    def filename   = "params_${timestamp}.json"
-    def temp_pf    = new File(workflow.launchDir.toString(), ".${filename}")
-    def jsonStr    = groovy.json.JsonOutput.toJson(params)
-    temp_pf.text   = groovy.json.JsonOutput.prettyPrint(jsonStr)
+    def timestamp = new java.util.Date().format('yyyy-MM-dd_HH-mm-ss')
+    def filename  = "params_${timestamp}.json"
+    def temp_pf   = new File(workflow.launchDir.toString(), ".${filename}")
+    def jsonStr   = groovy.json.JsonOutput.toJson(params)
+    temp_pf.text  = groovy.json.JsonOutput.prettyPrint(jsonStr)
 
     nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json")
     temp_pf.delete()
@@ -91,9 +90,14 @@ def checkCondaChannels() {
     try {
         def config = parser.load("conda config --show channels".execute().text)
         channels = config.channels
-    } catch(NullPointerException | IOException e) {
-        log.warn "Could not verify conda channel configuration."
-        return
+    }
+    catch (NullPointerException e) {
+        log.warn("Could not verify conda channel configuration.")
+        return null
+    }
+    catch (IOException e) {
+        log.warn("Could not verify conda channel configuration.")
+        return null
     }
 
     // Check that all channels are present
@@ -106,19 +110,13 @@ def checkCondaChannels() {
 
     required_channels_in_order.eachWithIndex { channel, index ->
         if (index < required_channels_in_order.size() - 1) {
-            channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index+1]))
+            channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index + 1]))
         }
     }
 
     if (channels_missing | channel_priority_violation) {
-        log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
-            "  There is a problem with your Conda configuration!\n\n" +
-            "  You will need to set-up the conda-forge and bioconda channels correctly.\n" +
-            "  Please refer to https://bioconda.github.io/\n" +
-            "  The observed channel order is \n" +
-            "  ${channels}\n" +
-            "  but the following channel order is required:\n" +
-            "  ${required_channels_in_order}\n" +
-            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+        log.warn(
+            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + "  There is a problem with your Conda configuration!\n\n" + "  You will need to set-up the conda-forge and bioconda channels correctly.\n" + "  Please refer to https://bioconda.github.io/\n" + "  The observed channel order is \n" + "  ${channels}\n" + "  but the following channel order is required:\n" + "  ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+        )
     }
 }
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index cbd8495bb..b78273ca4 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -3,13 +3,12 @@
 //
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     SUBWORKFLOW DEFINITION
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 workflow UTILS_NFCORE_PIPELINE {
-
     take:
     nextflow_cli_args
 
@@ -22,9 +21,9 @@ workflow UTILS_NFCORE_PIPELINE {
 }
 
 /*
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     FUNCTIONS
-========================================================================================
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 //
@@ -33,12 +32,9 @@ workflow UTILS_NFCORE_PIPELINE {
 def checkConfigProvided() {
     def valid_config = true as Boolean
     if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) {
-        log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" +
-            "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" +
-            "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" +
-            "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" +
-            "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" +
-            "Please refer to the quick start section and usage docs for the pipeline.\n "
+        log.warn(
+            "[${workflow.manifest.name}] You are attempting to run the pipeline without any custom configuration!\n\n" + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + "   (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + "   (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + "   (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + "Please refer to the quick start section and usage docs for the pipeline.\n "
+        )
         valid_config = false
     }
     return valid_config
@@ -49,12 +45,14 @@ def checkConfigProvided() {
 //
 def checkProfileProvided(nextflow_cli_args) {
     if (workflow.profile.endsWith(',')) {
-        error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" +
-            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+        error(
+            "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+        )
     }
     if (nextflow_cli_args[0]) {
-        log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" +
-            "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+        log.warn(
+            "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n"
+        )
     }
 }
 
@@ -70,13 +68,7 @@ def workflowCitation() {
     manifest_doi.each { doi_ref ->
         temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
     }
-    return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" +
-        "* The pipeline\n" +
-        temp_doi_ref + "\n" +
-        "* The nf-core framework\n" +
-        "  https://doi.org/10.1038/s41587-020-0439-x\n\n" +
-        "* Software dependencies\n" +
-        "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
+    return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + "* The nf-core framework\n" + "  https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
 }
 
 //
@@ -102,7 +94,7 @@ def getWorkflowVersion() {
 //
 def processVersionsFromYAML(yaml_file) {
     def yaml = new org.yaml.snakeyaml.Yaml()
-    def versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] }
+    def versions = yaml.load(yaml_file).collectEntries { k, v -> [k.tokenize(':')[-1], v] }
     return yaml.dumpAsMap(versions).trim()
 }
 
@@ -112,8 +104,8 @@ def processVersionsFromYAML(yaml_file) {
 def workflowVersionToYAML() {
     return """
     Workflow:
-        $workflow.manifest.name: ${getWorkflowVersion()}
-        Nextflow: $workflow.nextflow.version
+        ${workflow.manifest.name}: ${getWorkflowVersion()}
+        Nextflow: ${workflow.nextflow.version}
     """.stripIndent().trim()
 }
 
@@ -121,11 +113,7 @@ def workflowVersionToYAML() {
 // Get channel of software versions used in pipeline in YAML format
 //
 def softwareVersionsToYAML(ch_versions) {
-    return ch_versions
-                .unique()
-                .map { version -> processVersionsFromYAML(version) }
-                .unique()
-                .mix(Channel.of(workflowVersionToYAML()))
+    return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML()))
 }
 
 //
@@ -133,25 +121,31 @@ def softwareVersionsToYAML(ch_versions) {
 //
 def paramsSummaryMultiqc(summary_params) {
     def summary_section = ''
-    summary_params.keySet().each { group ->
-        def group_params = summary_params.get(group)  // This gets the parameters of that particular group
-        if (group_params) {
-            summary_section += "    <p style=\"font-size:110%\"><b>$group</b></p>\n"
-            summary_section += "    <dl class=\"dl-horizontal\">\n"
-            group_params.keySet().sort().each { param ->
-                summary_section += "        <dt>$param</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+    summary_params
+        .keySet()
+        .each { group ->
+            def group_params = summary_params.get(group)
+            // This gets the parameters of that particular group
+            if (group_params) {
+                summary_section += "    <p style=\"font-size:110%\"><b>${group}</b></p>\n"
+                summary_section += "    <dl class=\"dl-horizontal\">\n"
+                group_params
+                    .keySet()
+                    .sort()
+                    .each { param ->
+                        summary_section += "        <dt>${param}</dt><dd><samp>${group_params.get(param) ?: '<span style=\"color:#999999;\">N/A</a>'}</samp></dd>\n"
+                    }
+                summary_section += "    </dl>\n"
             }
-            summary_section += "    </dl>\n"
         }
-    }
 
-    def yaml_file_text  = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" as String
-    yaml_file_text        += "description: ' - this information is collected when the pipeline is started.'\n"
-    yaml_file_text        += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
-    yaml_file_text        += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
-    yaml_file_text        += "plot_type: 'html'\n"
-    yaml_file_text        += "data: |\n"
-    yaml_file_text        += "${summary_section}"
+    def yaml_file_text = "id: '${workflow.manifest.name.replace('/', '-')}-summary'\n" as String
+    yaml_file_text     += "description: ' - this information is collected when the pipeline is started.'\n"
+    yaml_file_text     += "section_name: '${workflow.manifest.name} Workflow Summary'\n"
+    yaml_file_text     += "section_href: 'https://github.com/${workflow.manifest.name}'\n"
+    yaml_file_text     += "plot_type: 'html'\n"
+    yaml_file_text     += "data: |\n"
+    yaml_file_text     += "${summary_section}"
 
     return yaml_file_text
 }
@@ -199,54 +193,54 @@ def logColours(monochrome_logs=true) {
     colorcodes['hidden']     = monochrome_logs ? '' : "\033[8m"
 
     // Regular Colors
-    colorcodes['black']      = monochrome_logs ? '' : "\033[0;30m"
-    colorcodes['red']        = monochrome_logs ? '' : "\033[0;31m"
-    colorcodes['green']      = monochrome_logs ? '' : "\033[0;32m"
-    colorcodes['yellow']     = monochrome_logs ? '' : "\033[0;33m"
-    colorcodes['blue']       = monochrome_logs ? '' : "\033[0;34m"
-    colorcodes['purple']     = monochrome_logs ? '' : "\033[0;35m"
-    colorcodes['cyan']       = monochrome_logs ? '' : "\033[0;36m"
-    colorcodes['white']      = monochrome_logs ? '' : "\033[0;37m"
+    colorcodes['black']  = monochrome_logs ? '' : "\033[0;30m"
+    colorcodes['red']    = monochrome_logs ? '' : "\033[0;31m"
+    colorcodes['green']  = monochrome_logs ? '' : "\033[0;32m"
+    colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m"
+    colorcodes['blue']   = monochrome_logs ? '' : "\033[0;34m"
+    colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m"
+    colorcodes['cyan']   = monochrome_logs ? '' : "\033[0;36m"
+    colorcodes['white']  = monochrome_logs ? '' : "\033[0;37m"
 
     // Bold
-    colorcodes['bblack']     = monochrome_logs ? '' : "\033[1;30m"
-    colorcodes['bred']       = monochrome_logs ? '' : "\033[1;31m"
-    colorcodes['bgreen']     = monochrome_logs ? '' : "\033[1;32m"
-    colorcodes['byellow']    = monochrome_logs ? '' : "\033[1;33m"
-    colorcodes['bblue']      = monochrome_logs ? '' : "\033[1;34m"
-    colorcodes['bpurple']    = monochrome_logs ? '' : "\033[1;35m"
-    colorcodes['bcyan']      = monochrome_logs ? '' : "\033[1;36m"
-    colorcodes['bwhite']     = monochrome_logs ? '' : "\033[1;37m"
+    colorcodes['bblack']  = monochrome_logs ? '' : "\033[1;30m"
+    colorcodes['bred']    = monochrome_logs ? '' : "\033[1;31m"
+    colorcodes['bgreen']  = monochrome_logs ? '' : "\033[1;32m"
+    colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m"
+    colorcodes['bblue']   = monochrome_logs ? '' : "\033[1;34m"
+    colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m"
+    colorcodes['bcyan']   = monochrome_logs ? '' : "\033[1;36m"
+    colorcodes['bwhite']  = monochrome_logs ? '' : "\033[1;37m"
 
     // Underline
-    colorcodes['ublack']     = monochrome_logs ? '' : "\033[4;30m"
-    colorcodes['ured']       = monochrome_logs ? '' : "\033[4;31m"
-    colorcodes['ugreen']     = monochrome_logs ? '' : "\033[4;32m"
-    colorcodes['uyellow']    = monochrome_logs ? '' : "\033[4;33m"
-    colorcodes['ublue']      = monochrome_logs ? '' : "\033[4;34m"
-    colorcodes['upurple']    = monochrome_logs ? '' : "\033[4;35m"
-    colorcodes['ucyan']      = monochrome_logs ? '' : "\033[4;36m"
-    colorcodes['uwhite']     = monochrome_logs ? '' : "\033[4;37m"
+    colorcodes['ublack']  = monochrome_logs ? '' : "\033[4;30m"
+    colorcodes['ured']    = monochrome_logs ? '' : "\033[4;31m"
+    colorcodes['ugreen']  = monochrome_logs ? '' : "\033[4;32m"
+    colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m"
+    colorcodes['ublue']   = monochrome_logs ? '' : "\033[4;34m"
+    colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m"
+    colorcodes['ucyan']   = monochrome_logs ? '' : "\033[4;36m"
+    colorcodes['uwhite']  = monochrome_logs ? '' : "\033[4;37m"
 
     // High Intensity
-    colorcodes['iblack']     = monochrome_logs ? '' : "\033[0;90m"
-    colorcodes['ired']       = monochrome_logs ? '' : "\033[0;91m"
-    colorcodes['igreen']     = monochrome_logs ? '' : "\033[0;92m"
-    colorcodes['iyellow']    = monochrome_logs ? '' : "\033[0;93m"
-    colorcodes['iblue']      = monochrome_logs ? '' : "\033[0;94m"
-    colorcodes['ipurple']    = monochrome_logs ? '' : "\033[0;95m"
-    colorcodes['icyan']      = monochrome_logs ? '' : "\033[0;96m"
-    colorcodes['iwhite']     = monochrome_logs ? '' : "\033[0;97m"
+    colorcodes['iblack']  = monochrome_logs ? '' : "\033[0;90m"
+    colorcodes['ired']    = monochrome_logs ? '' : "\033[0;91m"
+    colorcodes['igreen']  = monochrome_logs ? '' : "\033[0;92m"
+    colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m"
+    colorcodes['iblue']   = monochrome_logs ? '' : "\033[0;94m"
+    colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m"
+    colorcodes['icyan']   = monochrome_logs ? '' : "\033[0;96m"
+    colorcodes['iwhite']  = monochrome_logs ? '' : "\033[0;97m"
 
     // Bold High Intensity
-    colorcodes['biblack']    = monochrome_logs ? '' : "\033[1;90m"
-    colorcodes['bired']      = monochrome_logs ? '' : "\033[1;91m"
-    colorcodes['bigreen']    = monochrome_logs ? '' : "\033[1;92m"
-    colorcodes['biyellow']   = monochrome_logs ? '' : "\033[1;93m"
-    colorcodes['biblue']     = monochrome_logs ? '' : "\033[1;94m"
-    colorcodes['bipurple']   = monochrome_logs ? '' : "\033[1;95m"
-    colorcodes['bicyan']     = monochrome_logs ? '' : "\033[1;96m"
-    colorcodes['biwhite']    = monochrome_logs ? '' : "\033[1;97m"
+    colorcodes['biblack']  = monochrome_logs ? '' : "\033[1;90m"
+    colorcodes['bired']    = monochrome_logs ? '' : "\033[1;91m"
+    colorcodes['bigreen']  = monochrome_logs ? '' : "\033[1;92m"
+    colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m"
+    colorcodes['biblue']   = monochrome_logs ? '' : "\033[1;94m"
+    colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m"
+    colorcodes['bicyan']   = monochrome_logs ? '' : "\033[1;96m"
+    colorcodes['biwhite']  = monochrome_logs ? '' : "\033[1;97m"
 
     return colorcodes
 }
@@ -261,14 +255,15 @@ def attachMultiqcReport(multiqc_report) {
             mqc_report = multiqc_report.getVal()
             if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
                 if (mqc_report.size() > 1) {
-                    log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one"
+                    log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one")
                 }
                 mqc_report = mqc_report[0]
             }
         }
-    } catch (all) {
+    }
+    catch (Exception all) {
         if (multiqc_report) {
-            log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email"
+            log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email")
         }
     }
     return mqc_report
@@ -280,26 +275,35 @@ def attachMultiqcReport(multiqc_report) {
 def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) {
 
     // Set up the e-mail variables
-    def subject = "[$workflow.manifest.name] Successful: $workflow.runName"
+    def subject = "[${workflow.manifest.name}] Successful: ${workflow.runName}"
     if (!workflow.success) {
-        subject = "[$workflow.manifest.name] FAILED: $workflow.runName"
+        subject = "[${workflow.manifest.name}] FAILED: ${workflow.runName}"
     }
 
     def summary = [:]
-    summary_params.keySet().sort().each { group ->
-        summary << summary_params[group]
-    }
+    summary_params
+        .keySet()
+        .sort()
+        .each { group ->
+            summary << summary_params[group]
+        }
 
     def misc_fields = [:]
     misc_fields['Date Started']              = workflow.start
     misc_fields['Date Completed']            = workflow.complete
     misc_fields['Pipeline script file path'] = workflow.scriptFile
     misc_fields['Pipeline script hash ID']   = workflow.scriptId
-    if (workflow.repository) misc_fields['Pipeline repository Git URL']    = workflow.repository
-    if (workflow.commitId)   misc_fields['Pipeline repository Git Commit'] = workflow.commitId
-    if (workflow.revision)   misc_fields['Pipeline Git branch/tag']        = workflow.revision
-    misc_fields['Nextflow Version']           = workflow.nextflow.version
-    misc_fields['Nextflow Build']             = workflow.nextflow.build
+    if (workflow.repository) {
+        misc_fields['Pipeline repository Git URL']    = workflow.repository
+    }
+    if (workflow.commitId) {
+        misc_fields['Pipeline repository Git Commit'] = workflow.commitId
+    }
+    if (workflow.revision) {
+        misc_fields['Pipeline Git branch/tag']        = workflow.revision
+    }
+    misc_fields['Nextflow Version']          = workflow.nextflow.version
+    misc_fields['Nextflow Build']            = workflow.nextflow.build
     misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp
 
     def email_fields = [:]
@@ -337,7 +341,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
 
     // Render the sendmail template
     def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
-    def smail_fields           = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ]
+    def smail_fields           = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()]
     def sf                     = new File("${workflow.projectDir}/assets/sendmail_template.txt")
     def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
     def sendmail_html          = sendmail_template.toString()
@@ -346,30 +350,32 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     def colors = logColours(monochrome_logs) as Map
     if (email_address) {
         try {
-            if (plaintext_email) { throw new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') }
+            if (plaintext_email) {
+new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML')            }
             // Try to send HTML e-mail using sendmail
             def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
             sendmail_tf.withWriter { w -> w << sendmail_html }
-            [ 'sendmail', '-t' ].execute() << sendmail_html
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-"
-        } catch (all) {
+            ['sendmail', '-t'].execute() << sendmail_html
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-")
+        }
+        catch (Exception all) {
             // Catch failures and try with plaintext
-            def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ]
+            def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address]
             mail_cmd.execute() << email_html
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-"
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (mail)-")
         }
     }
 
     // Write summary e-mail HTML to a file
     def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html")
     output_hf.withWriter { w -> w << email_html }
-    nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html");
+    nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html")
     output_hf.delete()
 
     // Write summary e-mail TXT to a file
     def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt")
     output_tf.withWriter { w -> w << email_txt }
-    nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt");
+    nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt")
     output_tf.delete()
 }
 
@@ -380,12 +386,14 @@ def completionSummary(monochrome_logs=true) {
     def colors = logColours(monochrome_logs) as Map
     if (workflow.success) {
         if (workflow.stats.ignoredCount == 0) {
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-"
-        } else {
-            log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-"
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Pipeline completed successfully${colors.reset}-")
+        }
+        else {
+            log.info("-${colors.purple}[${workflow.manifest.name}]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-")
         }
-    } else {
-        log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-"
+    }
+    else {
+        log.info("-${colors.purple}[${workflow.manifest.name}]${colors.red} Pipeline completed with errors${colors.reset}-")
     }
 }
 
@@ -394,21 +402,30 @@ def completionSummary(monochrome_logs=true) {
 //
 def imNotification(summary_params, hook_url) {
     def summary = [:]
-    summary_params.keySet().sort().each { group ->
-        summary << summary_params[group]
-    }
+    summary_params
+        .keySet()
+        .sort()
+        .each { group ->
+            summary << summary_params[group]
+        }
 
     def misc_fields = [:]
-    misc_fields['start']                                = workflow.start
-    misc_fields['complete']                             = workflow.complete
-    misc_fields['scriptfile']                           = workflow.scriptFile
-    misc_fields['scriptid']                             = workflow.scriptId
-    if (workflow.repository) misc_fields['repository']  = workflow.repository
-    if (workflow.commitId)   misc_fields['commitid']    = workflow.commitId
-    if (workflow.revision)   misc_fields['revision']    = workflow.revision
-    misc_fields['nxf_version']                          = workflow.nextflow.version
-    misc_fields['nxf_build']                            = workflow.nextflow.build
-    misc_fields['nxf_timestamp']                        = workflow.nextflow.timestamp
+    misc_fields['start']          = workflow.start
+    misc_fields['complete']       = workflow.complete
+    misc_fields['scriptfile']     = workflow.scriptFile
+    misc_fields['scriptid']       = workflow.scriptId
+    if (workflow.repository) {
+        misc_fields['repository'] = workflow.repository
+    }
+    if (workflow.commitId) {
+        misc_fields['commitid']   = workflow.commitId
+    }
+    if (workflow.revision) {
+        misc_fields['revision']   = workflow.revision
+    }
+    misc_fields['nxf_version']    = workflow.nextflow.version
+    misc_fields['nxf_build']      = workflow.nextflow.build
+    misc_fields['nxf_timestamp']  = workflow.nextflow.timestamp
 
     def msg_fields = [:]
     msg_fields['version']      = getWorkflowVersion()
@@ -433,13 +450,13 @@ def imNotification(summary_params, hook_url) {
     def json_message  = json_template.toString()
 
     // POST
-    def post = new URL(hook_url).openConnection();
+    def post = new URL(hook_url).openConnection()
     post.setRequestMethod("POST")
     post.setDoOutput(true)
     post.setRequestProperty("Content-Type", "application/json")
-    post.getOutputStream().write(json_message.getBytes("UTF-8"));
-    def postRC = post.getResponseCode();
-    if (! postRC.equals(200)) {
-        log.warn(post.getErrorStream().getText());
+    post.getOutputStream().write(json_message.getBytes("UTF-8"))
+    def postRC = post.getResponseCode()
+    if (!postRC.equals(200)) {
+        log.warn(post.getErrorStream().getText())
     }
 }

From 5073208e98aeee573c296ff5dfdaf840eababe71 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 10 Oct 2024 13:32:46 +0200
Subject: [PATCH 053/887] remove fusiongdb

---
 CHANGELOG.md                                | 2 ++
 docs/usage.md                               | 2 +-
 modules/local/fusionreport/download/main.nf | 1 -
 3 files changed, 3 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fe350b46c..aa3eb2b3e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,6 +22,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Removed
 
+- Remove fusionGDB from documentation and fusion-report download stubs
+
 ## v3.0.2 - [2024-04-10]
 
 ### Added
diff --git a/docs/usage.md b/docs/usage.md
index 80ff457c5..180664a48 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -98,7 +98,7 @@ process {
 }
 ```
 
-The four `fusion-report` files: `cosmic.db`, `fusiongdb.db`, `fusiongdb2.db`, `mitelman.db`
+The four `fusion-report` files: `cosmic.db`, `fusiongdb2.db`, `mitelman.db`
 should then be copied into the HPC `<REFERENCE_PATH>/references/fusion_report_db`.
 
 #### Note about fusioncatcher references
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index ac288ade9..971db1b7f 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -28,7 +28,6 @@ process FUSIONREPORT_DOWNLOAD {
     """
     touch cosmic.db
     touch fusiongdb2.db
-    touch fusiongdb.db
     touch mitelman.db
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From 3de8b312040e68a4b4e9e1c59e84f86115db0623 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 10 Oct 2024 13:33:53 +0200
Subject: [PATCH 054/887] update changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index aa3eb2b3e..1e5a81e76 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,7 +22,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Removed
 
-- Remove fusionGDB from documentation and fusion-report download stubs
+- Remove fusionGDB from documentation and fusion-report download stubs [#503](https://github.com/nf-core/rnafusion/pull/503)
 
 ## v3.0.2 - [2024-04-10]
 

From 2c08d0cfefa83a7746ee146d44d1fea2cddb39ea Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 10 Oct 2024 14:09:25 +0200
Subject: [PATCH 055/887] remove fusiongdb

---
 docs/output.md | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 128a2f365..62859668a 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -18,7 +18,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 - [StringTie](#stringtie) - StringTie assembly
 - [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
 - [Samtools](#samtools) - SAM/BAM file manipulation
-- [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman, FusionGBD and FusionGDB2 databases
+- [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman, and FusionGDB2 databases
 - [FusionInspector](#fusionInspector) - Supervised analysis of fusion predictions from fusion-report, recover and re-score evidence for such predictions
 - [Arriba visualisation](#arriba-visualisation) - Arriba visualisation report for FusionInspector fusions
 - [Picard](#picard) - Collect QC metrics
@@ -50,7 +50,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
     - `human_v<version>` - dir with all references for fusioncatcher
   - `fusion_report_db`
     - `cosmic.db`
-    - `fusiongdb.db`
     - `fusiongdb2.db`
     - `mitelman.db`
   - `star` - dir with STAR index

From fedf17f056f7e40a955b53df49a169e277eb5df5 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Fri, 11 Oct 2024 12:38:33 +0000
Subject: [PATCH 056/887] Template update for nf-core/tools version 3.0.2

---
 .github/workflows/ci.yml                      | 60 +++++++++++++------
 .../workflows/template_version_comment.yml    | 21 ++++---
 .gitignore                                    |  1 +
 .nf-core.yml                                  |  2 +-
 main.nf                                       |  2 +-
 modules.json                                  |  6 +-
 modules/nf-core/multiqc/main.nf               |  2 +-
 nextflow.config                               |  4 +-
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  4 +-
 .../nf-core/utils_nextflow_pipeline/main.nf   | 30 +++++-----
 .../nf-core/utils_nfcore_pipeline/main.nf     | 10 ++--
 workflows/rnafusion.nf                        |  2 -
 12 files changed, 86 insertions(+), 58 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 30fd4427a..04fba0396 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -11,6 +11,8 @@ on:
 
 env:
   NXF_ANSI_LOG: false
+  NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
+  NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
 
 concurrency:
   group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}"
@@ -18,7 +20,7 @@ concurrency:
 
 jobs:
   test:
-    name: Run pipeline with test data
+    name: "Run pipeline with test data (${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }})"
     # Only run on push if this is the nf-core dev branch (merged PRs)
     if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}"
     runs-on: ubuntu-latest
@@ -27,33 +29,57 @@ jobs:
         NXF_VER:
           - "24.04.2"
           - "latest-everything"
+        profile:
+          - "conda"
+          - "docker"
+          - "singularity"
+        test_name:
+          - "test"
+        isMaster:
+          - ${{ github.base_ref == 'master' }}
+        # Exclude conda and singularity on dev
+        exclude:
+          - isMaster: false
+            profile: "conda"
+          - isMaster: false
+            profile: "singularity"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
 
-      - name: Install Nextflow
+      - name: Set up Nextflow
         uses: nf-core/setup-nextflow@v2
         with:
           version: "${{ matrix.NXF_VER }}"
 
-      - name: Disk space cleanup
-        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+      - name: Set up Apptainer
+        if: matrix.profile == 'singularity'
+        uses: eWaterCycle/setup-apptainer@main
 
-      - name: Run pipeline with test data (docker)
-        # TODO nf-core: You can customise CI pipeline run tests as required
-        # For example: adding multiple test runs with different parameters
-        # Remember that you can parallelise this by using strategy.matrix
+      - name: Set up Singularity
+        if: matrix.profile == 'singularity'
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
+          mkdir -p $NXF_SINGULARITY_CACHEDIR
+          mkdir -p $NXF_SINGULARITY_LIBRARYDIR
+
+      - name: Set up Miniconda
+        if: matrix.profile == 'conda'
+        uses: conda-incubator/setup-miniconda@a4260408e20b96e80095f42ff7f1a15b27dd94ca # v3
+        with:
+          miniconda-version: "latest"
+          auto-update-conda: true
+          conda-solver: libmamba
+          channels: conda-forge,bioconda
 
-      - name: Run pipeline with test data (singularity)
-        # TODO nf-core: You can customise CI pipeline run tests as required
+      - name: Set up Conda
+        if: matrix.profile == 'conda'
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results
-        if: "${{ github.base_ref == 'master' }}"
+          echo $(realpath $CONDA)/condabin >> $GITHUB_PATH
+          echo $(realpath python) >> $GITHUB_PATH
+
+      - name: Clean up Disk space
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - name: Run pipeline with test data (conda)
-        # TODO nf-core: You can customise CI pipeline run tests as required
+      - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile test,conda --outdir ./results
-        if: "${{ github.base_ref == 'master' }}"
+          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} --outdir ./results
diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml
index 9dea41f0d..e8aafe44d 100644
--- a/.github/workflows/template_version_comment.yml
+++ b/.github/workflows/template_version_comment.yml
@@ -10,9 +10,11 @@ jobs:
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+        with:
+          ref: ${{ github.event.pull_request.head.sha }}
 
       - name: Read template version from .nf-core.yml
-        uses: pietrobolcato/action-read-yaml@1.0.0
+        uses: nichmor/minimal-read-yaml@v0.0.2
         id: read_yml
         with:
           config: ${{ github.workspace }}/.nf-core.yml
@@ -24,20 +26,21 @@ jobs:
 
       - name: Check nf-core outdated
         id: nf_core_outdated
-        run: pip list --outdated | grep nf-core
+        run: echo "OUTPUT=$(pip list --outdated | grep nf-core)" >> ${GITHUB_ENV}
 
       - name: Post nf-core template version comment
         uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
         if: |
-          ${{ steps.nf_core_outdated.outputs.stdout }} =~ 'nf-core'
+          contains(env.OUTPUT, 'nf-core')
         with:
           repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }}
           allow-repeats: false
           message: |
-            ## :warning: Newer version of the nf-core template is available.
-
-            Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}.
-            Please update your pipeline to the latest version.
-
-            For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync).
+            > [!WARNING]
+            > Newer version of the nf-core template is available.
+            >
+            > Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}.
+            > Please update your pipeline to the latest version.
+            >
+            > For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync).
           #
diff --git a/.gitignore b/.gitignore
index 5124c9ac7..a42ce0162 100644
--- a/.gitignore
+++ b/.gitignore
@@ -6,3 +6,4 @@ results/
 testing/
 testing*
 *.pyc
+null/
diff --git a/.nf-core.yml b/.nf-core.yml
index d7c8a21e4..097a52b0e 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -3,7 +3,7 @@ lint:
   files_unchanged:
     - .github/CONTRIBUTING.md
     - .github/PULL_REQUEST_TEMPLATE.md
-nf_core_version: 3.0.1
+nf_core_version: 3.0.2
 org_path: null
 repository_type: pipeline
 template:
diff --git a/main.nf b/main.nf
index 3941f007e..6532daeed 100644
--- a/main.nf
+++ b/main.nf
@@ -76,7 +76,7 @@ workflow {
         params.outdir,
         params.input
     )
-    
+
     //
     // WORKFLOW: Run main workflow
     //
diff --git a/modules.json b/modules.json
index 243717102..01a6c107b 100644
--- a/modules.json
+++ b/modules.json
@@ -12,7 +12,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "b8d36829fa84b6e404364abff787e8b07f6d058c",
+                        "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d",
                         "installed_by": ["modules"]
                     }
                 }
@@ -21,12 +21,12 @@
                 "nf-core": {
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "9d05360da397692321d377b6102d2fb22507c6ef",
+                        "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "772684d9d66f37b650c8ba5146ac1ee3ecba2acb",
+                        "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 9724d2f34..cc0643e1d 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -52,7 +52,7 @@ process MULTIQC {
     stub:
     """
     mkdir multiqc_data
-    touch multiqc_plots
+    mkdir multiqc_plots
     touch multiqc_report.html
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/nextflow.config b/nextflow.config
index d6f438520..872c08044 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -254,10 +254,10 @@ validation {
 """
         afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { "  https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""}
 * The nf-core framework
-  https://doi.org/10.1038/s41587-020-0439-x
+    https://doi.org/10.1038/s41587-020-0439-x
 
 * Software dependencies
-  https://github.com/${manifest.name}/blob/master/CITATIONS.md
+    https://github.com/${manifest.name}/blob/master/CITATIONS.md
 """
     }
     summary {
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 71a09c15b..834a4e5c6 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -47,7 +47,6 @@ workflow PIPELINE_INITIALISATION {
         workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1
     )
 
-    
     //
     // Validate parameters and generate parameter summary to stdout
     //
@@ -56,7 +55,6 @@ workflow PIPELINE_INITIALISATION {
         validate_params,
         null
     )
-    
 
     //
     // Check config provided to the pipeline
@@ -64,6 +62,7 @@ workflow PIPELINE_INITIALISATION {
     UTILS_NFCORE_PIPELINE (
         nextflow_cli_args
     )
+
     //
     // Custom validation for pipeline parameters
     //
@@ -110,7 +109,6 @@ workflow PIPELINE_COMPLETION {
     email           //  string: email address
     email_on_fail   //  string: email address sent on pipeline failure
     plaintext_email // boolean: Send plain-text email instead of HTML
-    
     outdir          //    path: Path to output directory where results will be published
     monochrome_logs // boolean: Disable ANSI colour codes in log output
     hook_url        //  string: hook URL for notifications
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
index 2b0dc67a6..0fcbf7b3f 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -3,9 +3,9 @@
 //
 
 /*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     SUBWORKFLOW DEFINITION
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 workflow UTILS_NEXTFLOW_PIPELINE {
@@ -44,9 +44,9 @@ workflow UTILS_NEXTFLOW_PIPELINE {
 }
 
 /*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     FUNCTIONS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 //
@@ -106,17 +106,19 @@ def checkCondaChannels() {
     def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean
 
     // Check that they are in the right order
-    def channel_priority_violation = false
-
-    required_channels_in_order.eachWithIndex { channel, index ->
-        if (index < required_channels_in_order.size() - 1) {
-            channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index + 1]))
-        }
-    }
+    def channel_priority_violation = required_channels_in_order != channels.findAll { ch -> ch in required_channels_in_order }
 
     if (channels_missing | channel_priority_violation) {
-        log.warn(
-            "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + "  There is a problem with your Conda configuration!\n\n" + "  You will need to set-up the conda-forge and bioconda channels correctly.\n" + "  Please refer to https://bioconda.github.io/\n" + "  The observed channel order is \n" + "  ${channels}\n" + "  but the following channel order is required:\n" + "  ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
-        )
+        log.warn """\
+        ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+            There is a problem with your Conda configuration!
+            You will need to set-up the conda-forge and bioconda channels correctly.
+            Please refer to https://bioconda.github.io/
+            The observed channel order is
+            ${channels}
+            but the following channel order is required:
+            ${required_channels_in_order}
+        ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
+        """.stripIndent(true)
     }
 }
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index b78273ca4..5cb7bafef 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -3,9 +3,9 @@
 //
 
 /*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     SUBWORKFLOW DEFINITION
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 workflow UTILS_NFCORE_PIPELINE {
@@ -21,9 +21,9 @@ workflow UTILS_NFCORE_PIPELINE {
 }
 
 /*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     FUNCTIONS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
 //
@@ -62,7 +62,7 @@ def checkProfileProvided(nextflow_cli_args) {
 def workflowCitation() {
     def temp_doi_ref = ""
     def manifest_doi = workflow.manifest.doi.tokenize(",")
-    // Using a loop to handle multiple DOIs
+    // Handling multiple DOIs
     // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
     // Removing ` ` since the manifest.doi is a string and not a proper list
     manifest_doi.each { doi_ref ->
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 8192c8229..53c51b00d 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -57,13 +57,11 @@ workflow RNAFUSION {
         Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
         Channel.empty()
 
-    
     summary_params      = paramsSummaryMap(
         workflow, parameters_schema: "nextflow_schema.json")
     ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
     ch_multiqc_files = ch_multiqc_files.mix(
         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    
     ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
         file(params.multiqc_methods_description, checkIfExists: true) :
         file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)

From 7a889713da5ee69696daf159cb45d94e577cc483 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 09:27:26 +0200
Subject: [PATCH 057/887] ensembl to gencode, removing chrgtf

---
 conf/modules.config                           |  11 +-
 modules.json                                  |  10 ++
 modules/local/ensembl/main.nf                 |  55 -------
 modules/local/fusioncatcher/build/main.nf     |  12 +-
 modules/local/gencode_download/main.nf        |  49 +++++++
 modules/local/hgnc/main.nf                    |   2 +-
 modules/local/starfusion/download/main.nf     |   1 -
 .../nf-core/arriba/download/environment.yml   |   5 +
 modules/nf-core/arriba/download/main.nf       |  46 ++++++
 modules/nf-core/arriba/download/meta.yml      |  30 ++++
 .../arriba/download/tests/main.nf.test        |  35 +++++
 .../arriba/download/tests/main.nf.test.snap   |  35 +++++
 modules/nf-core/gunzip/environment.yml        |   7 +
 modules/nf-core/gunzip/main.nf                |  55 +++++++
 modules/nf-core/gunzip/meta.yml               |  47 ++++++
 modules/nf-core/gunzip/tests/main.nf.test     | 121 ++++++++++++++++
 .../nf-core/gunzip/tests/main.nf.test.snap    | 134 ++++++++++++++++++
 modules/nf-core/gunzip/tests/nextflow.config  |   5 +
 modules/nf-core/gunzip/tests/tags.yml         |   2 +
 nextflow.config                               |  19 ++-
 nextflow_schema.json                          |  19 +--
 subworkflows/local/build_references.nf        |  96 ++++++-------
 subworkflows/local/qc_workflow.nf             |   2 +-
 subworkflows/local/starfusion_workflow.nf     |   4 +-
 subworkflows/local/stringtie_workflow.nf      |   6 +-
 workflows/rnafusion.nf                        |  10 +-
 26 files changed, 663 insertions(+), 155 deletions(-)
 delete mode 100644 modules/local/ensembl/main.nf
 create mode 100644 modules/local/gencode_download/main.nf
 create mode 100644 modules/nf-core/arriba/download/environment.yml
 create mode 100644 modules/nf-core/arriba/download/main.nf
 create mode 100644 modules/nf-core/arriba/download/meta.yml
 create mode 100644 modules/nf-core/arriba/download/tests/main.nf.test
 create mode 100644 modules/nf-core/arriba/download/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/gunzip/environment.yml
 create mode 100644 modules/nf-core/gunzip/main.nf
 create mode 100644 modules/nf-core/gunzip/meta.yml
 create mode 100644 modules/nf-core/gunzip/tests/main.nf.test
 create mode 100644 modules/nf-core/gunzip/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/gunzip/tests/nextflow.config
 create mode 100644 modules/nf-core/gunzip/tests/tags.yml

diff --git a/conf/modules.config b/conf/modules.config
index bbafa80d6..af8324983 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -49,10 +49,9 @@ process {
         ext.when   = { !params.build_references }
     }
 
-    withName: '.*BUILD_REFERENCES:ENSEMBL_DOWNLOAD' {
-        ext.when = { !params.fasta || ! params.gtf }
+    withName: 'GENCODE_DOWNLOAD' {
         publishDir = [
-            path: { "${params.genomes_base}/ensembl" },
+            path: { "${params.genomes_base}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -122,7 +121,7 @@ process {
 
     withName: 'GATK4_BEDTOINTERVALLIST' {
         publishDir = [
-            path: { "${params.genomes_base}/ensembl" },
+            path: { "${params.genomes_base}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -139,7 +138,7 @@ process {
 
     withName: 'GTF_TO_REFFLAT' {
         publishDir = [
-            path: { "${params.genomes_base}/ensembl" },
+            path: { "${params.genomes_base}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -209,7 +208,7 @@ process {
 
     withName: 'SAMTOOLS_FAIDX' {
         publishDir = [
-            path: { "${params.genomes_base}/ensembl" },
+            path: { "${params.genomes_base}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
diff --git a/modules.json b/modules.json
index 7f4cf0efc..886af9756 100644
--- a/modules.json
+++ b/modules.json
@@ -15,6 +15,11 @@
                         "git_sha": "bbaeb3a3149def237b9d0acb9ab749ae98088e29",
                         "installed_by": ["modules"]
                     },
+                    "arriba/download": {
+                        "branch": "master",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "installed_by": ["modules"]
+                    },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
@@ -55,6 +60,11 @@
                         "git_sha": "6c996d7fbe0816dcbb68ce587ad5f873313682a1",
                         "installed_by": ["modules"]
                     },
+                    "gunzip": {
+                        "branch": "master",
+                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "installed_by": ["modules"]
+                    },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a",
diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
deleted file mode 100644
index 695ab3123..000000000
--- a/modules/local/ensembl/main.nf
+++ /dev/null
@@ -1,55 +0,0 @@
-process ENSEMBL_DOWNLOAD {
-    tag "ensembl"
-    label 'process_low'
-
-    conda "bioconda::gnu-wget=1.18"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
-        'quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
-
-    input:
-    val ensembl_version
-    val genome
-    val meta
-
-    output:
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.gtf")                       , emit: gtf
-    tuple val(meta), path("Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa")   , emit: fasta
-    path "versions.yml"                                                                          , emit: versions
-
-
-    script:
-    """
-    if [ ${genome} == 'GRCh37' ]; then
-        wget ftp://ftp.ensembl.org/pub/grch37/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${genome}.87.gtf.gz -O Homo_sapiens.${genome}.${ensembl_version}.gtf.gz
-        wget ftp://ftp.ensembl.org/pub/grch37/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${genome}.dna.primary_assembly.fa.gz -O Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz
-
-
-    else:
-        wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${genome}.${ensembl_version}.gtf.gz
-        wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${genome}.dna.primary_assembly.fa.gz -O Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz
-
-    gunzip Homo_sapiens.${genome}.${ensembl_version}.gtf.gz
-    gunzip Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch "Homo_sapiens.${genome}.${ensembl_version}.gtf"
-    touch "Homo_sapiens.${genome}.${ensembl_version}.dna.primary_assembly.fa"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-    END_VERSIONS
-    """
-
-}
diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 15f7afedc..51153253a 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -6,11 +6,11 @@ process FUSIONCATCHER_BUILD {
     container "docker.io/clinicalgenomics/fusioncatcher:1.33"
 
     input:
-    val ensembl_version
+    val genome_gencode_version
 
     output:
-    path "human_v${ensembl_version}"  , emit: reference
-    path "versions.yml"               , emit: versions
+    path "human_v${genome_gencode_version}"  , emit: reference
+    path "versions.yml"                      , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -21,7 +21,7 @@ process FUSIONCATCHER_BUILD {
     """
     fusioncatcher-build.py \\
         -g homo_sapiens \\
-        -o human_v${ensembl_version} \\
+        -o human_v${genome_gencode_version} \\
         $args
 
     cat <<-END_VERSIONS > versions.yml
@@ -32,8 +32,8 @@ process FUSIONCATCHER_BUILD {
 
     stub:
     """
-    mkdir human_v${ensembl_version}
-    touch human_v${ensembl_version}/ensembl_fully_overlapping_genes.txt
+    mkdir human_v${genome_gencode_version}
+    touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
diff --git a/modules/local/gencode_download/main.nf b/modules/local/gencode_download/main.nf
new file mode 100644
index 000000000..e9416cf4d
--- /dev/null
+++ b/modules/local/gencode_download/main.nf
@@ -0,0 +1,49 @@
+process GENCODE_DOWNLOAD {
+    tag "gencode_download"
+    label 'process_low'
+
+    conda "bioconda::gnu-wget=1.18"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
+        'quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
+
+    input:
+    val genome_gencode_version
+    val genome
+
+    output:
+    path "*.fa"        , emit: fasta
+    path "*.gtf"       , emit: gtf
+    path "versions.yml", emit: versions
+
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def folder_gencode = genome.contains("38") ? "" : "/${genome}_mapping"
+    def gtf_file_name = genome.contains("38") ? "gencode.v${genome_gencode_version}.primary_assembly.annotation.gtf.gz" : "gencode.v${genome_gencode_version}lift${genome_gencode_version}.annotation.gtf.gz"
+    """
+    wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_${genome_gencode_version}/${folder_gencode}${genome}.primary_assembly.genome.fa.gz -O Homo_sapiens_${genome}_${genome_gencode_version}_dna_primary_assembly.fa.gz
+    gunzip Homo_sapiens_${genome}_${genome_gencode_version}_dna_primary_assembly.fa.gz
+    wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_${genome_gencode_version}/${folder_gencode}${gtf_file_name} -O Homo_sapiens_${genome}_${genome_gencode_version}.gtf.gz
+    gunzip  Homo_sapiens_${genome}_${genome_gencode_version}.gtf.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch Homo_sapiens.${genome}.${genome_gencode_version}_dna_primary_assembly.fa
+    touch Homo_sapiens.${genome}.${genome_gencode_version}.gtf
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+    END_VERSIONS
+    """
+
+}
diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 1b3808f6d..6a0556314 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -18,7 +18,7 @@ process HGNC_DOWNLOAD {
 
     script:
     """
-    wget https://ftp.ebi.ac.uk/pub/databases/genenames/hgnc/tsv/hgnc_complete_set.txt
+    wget https://ftp.ebi.ac.uk/pub/databases/genenames/out_of_date_hgnc/tsv/hgnc_complete_set.txt
     date +%Y-%m-%d/%H:%M  > HGNC-DB-timestamp.txt
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index fedadfa0b..17bfa72eb 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -6,7 +6,6 @@ process STARFUSION_DOWNLOAD {
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference
-    path "ctat_genome_lib_build_dir/ref_annot.gtf", emit: chrgtf
 
 
     // TODO: move to my sourceforge
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
new file mode 100644
index 000000000..d0883a0d2
--- /dev/null
+++ b/modules/nf-core/arriba/download/environment.yml
@@ -0,0 +1,5 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::arriba=2.4.0
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
new file mode 100644
index 000000000..96a4e3362
--- /dev/null
+++ b/modules/nf-core/arriba/download/main.nf
@@ -0,0 +1,46 @@
+process ARRIBA_DOWNLOAD {
+    tag "arriba"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
+        'biocontainers/arriba:2.4.0--h0033a41_2' }"
+
+    input:
+
+    output:
+    path "*"              , emit: reference
+    path "versions.yml"           , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz
+    tar -xzvf arriba_v2.4.0.tar.gz
+    rm arriba_v2.4.0.tar.gz
+    mv arriba_v2.4.0/database/* .
+    rm -r arriba_v2.4.0
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        arriba_download: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch blacklist_hg38_GRCh38_v2.4.0.tsv.gz
+    touch protein_domains_hg38_GRCh38_v2.4.0.gff3
+    touch cytobands_hg38_GRCh38_v2.4.0.tsv
+    touch known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
+    touch protein_domains_hg38_GRCh38_v2.4.0.gff3
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        arriba_download: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
new file mode 100644
index 000000000..531c66c2f
--- /dev/null
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -0,0 +1,30 @@
+name: arriba_download
+description: Arriba is a command-line tool for the detection of gene fusions from
+  RNA-Seq data.
+keywords:
+  - fusion
+  - arriba
+  - reference
+tools:
+  - arriba:
+      description: Fast and accurate gene fusion detection from RNA-Seq data
+      homepage: https://github.com/suhrig/arriba
+      documentation: https://arriba.readthedocs.io/en/latest/
+      tool_dev_url: https://github.com/suhrig/arriba
+      doi: "10.1101/gr.257246.119"
+      licence: ["MIT"]
+      identifier: biotools:Arriba
+
+output:
+  - reference:
+      - "*":
+          type: directory
+          description: Folder with arriba references
+          pattern: "*"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@peterpru"
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test b/modules/nf-core/arriba/download/tests/main.nf.test
new file mode 100644
index 000000000..cccc95db2
--- /dev/null
+++ b/modules/nf-core/arriba/download/tests/main.nf.test
@@ -0,0 +1,35 @@
+
+nextflow_process {
+
+    name "Test Process ARRIBA_DOWNLOAD"
+    script "../main.nf"
+    process "ARRIBA_DOWNLOAD"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "arriba"
+    tag "arriba/download"
+
+    test("test-arriba-download") {
+
+        when {
+            process {
+                """
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.reference[0].collect { file(it).name }.toSorted(),
+                    process.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
new file mode 100644
index 000000000..eda3f7066
--- /dev/null
+++ b/modules/nf-core/arriba/download/tests/main.nf.test.snap
@@ -0,0 +1,35 @@
+{
+    "test-arriba-download": {
+        "content": [
+            [
+                "CREDITS",
+                "RefSeq_viral_genomes_v2.4.0.fa.gz",
+                "blacklist_hg19_hs37d5_GRCh37_v2.4.0.tsv.gz",
+                "blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "blacklist_mm10_GRCm38_v2.4.0.tsv.gz",
+                "blacklist_mm39_GRCm39_v2.4.0.tsv.gz",
+                "cytobands_hg19_hs37d5_GRCh37_v2.4.0.tsv",
+                "cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "cytobands_mm10_GRCm38_v2.4.0.tsv",
+                "cytobands_mm39_GRCm39_v2.4.0.tsv",
+                "known_fusions_hg19_hs37d5_GRCh37_v2.4.0.tsv.gz",
+                "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "known_fusions_mm10_GRCm38_v2.4.0.tsv.gz",
+                "known_fusions_mm39_GRCm39_v2.4.0.tsv.gz",
+                "protein_domains_hg19_hs37d5_GRCh37_v2.4.0.gff3",
+                "protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "protein_domains_mm10_GRCm38_v2.4.0.gff3",
+                "protein_domains_mm39_GRCm39_v2.4.0.gff3",
+                "versions.yml"
+            ],
+            [
+                "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-22T17:00:38.086459"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml
new file mode 100644
index 000000000..c7794856d
--- /dev/null
+++ b/modules/nf-core/gunzip/environment.yml
@@ -0,0 +1,7 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - conda-forge::grep=3.11
+  - conda-forge::sed=4.8
+  - conda-forge::tar=1.34
diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf
new file mode 100644
index 000000000..5e67e3b9b
--- /dev/null
+++ b/modules/nf-core/gunzip/main.nf
@@ -0,0 +1,55 @@
+process GUNZIP {
+    tag "$archive"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/ubuntu:22.04' :
+        'nf-core/ubuntu:22.04' }"
+
+    input:
+    tuple val(meta), path(archive)
+
+    output:
+    tuple val(meta), path("$gunzip"), emit: gunzip
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args        = task.ext.args ?: ''
+    def extension   = ( archive.toString() - '.gz' ).tokenize('.')[-1]
+    def name        = archive.toString() - '.gz' - ".$extension"
+    def prefix      = task.ext.prefix ?: name
+    gunzip          = prefix + ".$extension"
+    """
+    # Not calling gunzip itself because it creates files
+    # with the original group ownership rather than the
+    # default one for that user / the work directory
+    gzip \\
+        -cd \\
+        $args \\
+        $archive \\
+        > $gunzip
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def args        = task.ext.args ?: ''
+    def extension   = ( archive.toString() - '.gz' ).tokenize('.')[-1]
+    def name        = archive.toString() - '.gz' - ".$extension"
+    def prefix      = task.ext.prefix ?: name
+    gunzip          = prefix + ".$extension"
+    """
+    touch $gunzip
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml
new file mode 100644
index 000000000..9066c0353
--- /dev/null
+++ b/modules/nf-core/gunzip/meta.yml
@@ -0,0 +1,47 @@
+name: gunzip
+description: Compresses and decompresses files.
+keywords:
+  - gunzip
+  - compression
+  - decompression
+tools:
+  - gunzip:
+      description: |
+        gzip is a file format and a software application used for file compression and decompression.
+      documentation: https://www.gnu.org/software/gzip/manual/gzip.html
+      licence: ["GPL-3.0-or-later"]
+      identifier: ""
+input:
+  - - meta:
+        type: map
+        description: |
+          Optional groovy Map containing meta information
+          e.g. [ id:'test', single_end:false ]
+    - archive:
+        type: file
+        description: File to be compressed/uncompressed
+        pattern: "*.*"
+output:
+  - gunzip:
+      - meta:
+          type: file
+          description: Compressed/uncompressed file
+          pattern: "*.*"
+      - $gunzip:
+          type: file
+          description: Compressed/uncompressed file
+          pattern: "*.*"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@jfy133"
+maintainers:
+  - "@joseespinosa"
+  - "@drpatelh"
+  - "@jfy133"
+  - "@gallvp"
diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test
new file mode 100644
index 000000000..776211adf
--- /dev/null
+++ b/modules/nf-core/gunzip/tests/main.nf.test
@@ -0,0 +1,121 @@
+nextflow_process {
+
+    name "Test Process GUNZIP"
+    script "../main.nf"
+    process "GUNZIP"
+    tag "gunzip"
+    tag "modules_nfcore"
+    tag "modules"
+
+    test("Should run without failures") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                        [],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                    ]
+                )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("Should run without failures - prefix") {
+
+        config './nextflow.config'
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                        [ id: 'test' ],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                    ]
+                )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("Should run without failures - stub") {
+
+        options '-stub'
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                        [],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                    ]
+                )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("Should run without failures - prefix - stub") {
+
+        options '-stub'
+        config './nextflow.config'
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([
+                        [ id: 'test' ],
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
+                    ]
+                )
+                """
+            }
+        }
+
+        then {
+            assertAll(
+            { assert process.success },
+            { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap
new file mode 100644
index 000000000..069967e76
--- /dev/null
+++ b/modules/nf-core/gunzip/tests/main.nf.test.snap
@@ -0,0 +1,134 @@
+{
+    "Should run without failures - prefix - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ],
+                "gunzip": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-06-25T11:35:10.861293"
+    },
+    "Should run without failures - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        [
+                            
+                        ],
+                        "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ],
+                "gunzip": [
+                    [
+                        [
+                            
+                        ],
+                        "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-06-25T11:35:05.857145"
+    },
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    [
+                        [
+                            
+                        ],
+                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ],
+                "gunzip": [
+                    [
+                        [
+                            
+                        ],
+                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2023-10-17T15:35:37.690477896"
+    },
+    "Should run without failures - prefix": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ],
+                "gunzip": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-06-25T11:33:32.921739"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/gunzip/tests/nextflow.config b/modules/nf-core/gunzip/tests/nextflow.config
new file mode 100644
index 000000000..dec776425
--- /dev/null
+++ b/modules/nf-core/gunzip/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: GUNZIP {
+        ext.prefix = { "${meta.id}.xyz" }
+    }
+}
diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml
new file mode 100644
index 000000000..fd3f69154
--- /dev/null
+++ b/modules/nf-core/gunzip/tests/tags.yml
@@ -0,0 +1,2 @@
+gunzip:
+  - modules/nf-core/gunzip/**
diff --git a/nextflow.config b/nextflow.config
index db5a3f633..63369d6c4 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -12,7 +12,6 @@ params {
     // Input options
     input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
 
-    build_references           = false
     cosmic_username            = null
     cosmic_passwd              = null
     qiagen                     = false
@@ -27,7 +26,7 @@ params {
     // Genome
     genome                     = 'GRCh38'
     genomes_base               = "${params.outdir}/references"
-    ensembl_version            = 112
+    genome_gencode_version     = 46
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
@@ -69,16 +68,16 @@ params {
     download_refs              = true
 
     // Path to references
-    fasta                         = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa"
-    fai                           = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.dna.primary_assembly.fa.fai"
-    gtf                           = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf"
-    refflat                       = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf.refflat"
-    rrna_intervals                = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list"
-    ensembl_ref                   = "${params.genomes_base}/ensembl"
+    fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"
+    fai                           = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa.fai"
+    gtf                           = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf"
+    refflat                       = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf.refflat"
+    rrna_intervals                = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.interval_list"
+    gencode_ref                   = "${params.genomes_base}/gencode"
     arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz"
     arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz"
     arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3"
-    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.ensembl_version}"
+    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}"
     hgnc_ref                      = "${params.genomes_base}/hgnc/hgnc_complete_set.txt"
     hgnc_date                     = "${params.genomes_base}/hgnc/HGNC-DB-timestamp.txt"
     salmon_index                  = "${params.genomes_base}/salmon/salmon"
@@ -91,7 +90,7 @@ params {
     salmon_index_stub_check       = "${params.genomes_base}/salmon/salmon/complete_ref_lens.bin"
     starindex_ref_stub_check      = "${params.genomes_base}/star/star/Genome"
     fusionreport_ref_stub_check   = "${params.genomes_base}/fusion_report_db/mitelman.db"
-    fusioncatcher_ref_stub_check  = "${params.genomes_base}/fusioncatcher/human_v${params.ensembl_version}/ensembl_fully_overlapping_genes.txt"
+    fusioncatcher_ref_stub_check  = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}/ensembl_fully_overlapping_genes.txt"
     starfusion_ref_stub_check     = "${params.genomes_base}/starfusion/Pfam-A.hmm"
 
     // Path to fusion outputs
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 95238d65d..be31bcc30 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -62,11 +62,6 @@
                     "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.",
                     "fa_icon": "fas fa-file-signature"
                 },
-                "build_references": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Specifies which analysis type for the pipeline - either build references or analyse data"
-                },
                 "cosmic_username": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -82,7 +77,7 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to reference folder"
                 },
-                "ensembl_version": {
+                "genome_gencode_version": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
                     "description": "ensembl version",
@@ -134,10 +129,10 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Download references instead of building them (for fusioncatcher and starfusion)"
                 },
-                "ensembl_ref": {
+                "gencode_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
-                    "description": "Path to ensembl references"
+                    "description": "Path to gencode references"
                 },
                 "fusioncatcher": {
                     "type": "boolean",
@@ -342,14 +337,6 @@
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
                 },
-                "chrgtf": {
-                    "type": "string",
-                    "format": "file-path",
-                    "mimetype": "text/plain",
-                    "pattern": "^\\S+\\.gtf?(\\.gz)?$",
-                    "description": "Path to GTF genome file.",
-                    "fa_icon": "far fa-file-code"
-                },
                 "refflat": {
                     "type": "string",
                     "format": "file-path",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index cfb4258ba..8017f6346 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -5,7 +5,7 @@
 */
 
 include { ARRIBA_DOWNLOAD }                 from '../../modules/local/arriba/download/main'
-include { ENSEMBL_DOWNLOAD }                from '../../modules/local/ensembl/main'
+include { GENCODE_DOWNLOAD }                from '../../modules/local/gencode_download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../../modules/local/fusioncatcher/download/main'
 include { FUSIONCATCHER_BUILD }             from '../../modules/local/fusioncatcher/build/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../../modules/local/fusionreport/download/main'
@@ -37,26 +37,24 @@ include { GFFREAD }                         from '../../modules/nf-core/gffread/
 workflow BUILD_REFERENCES {
     take:
         genome                           // channel: [mandatory]  val(genome)
-        ensembl_version                  // channel: [mandatory]  val(ensembl_version)
+        genome_gencode_version           // channel: [mandatory]  val(genome_gencode_version)
 
     main:
     ch_versions = Channel.empty()
 
     if (!file(params.fasta).exists() || file(params.fasta).isEmpty() ||
             !file(params.gtf).exists() || file(params.gtf).isEmpty()){
-        fake_meta = [:]
-        fake_meta.id = "Homo_sapiens.${params.genome}.${params.ensembl_version}"
-        ENSEMBL_DOWNLOAD(params.ensembl_version, params.genome, fake_meta)
-        ch_versions = ENSEMBL_DOWNLOAD.out.versions
-        ch_fasta = ENSEMBL_DOWNLOAD.out.primary_assembly
-        ch_gtf = ENSEMBL_DOWNLOAD.out.gtf
+        GENCODE_DOWNLOAD(params.genome_gencode_version, params.genome)
+        ch_versions = GENCODE_DOWNLOAD.out.versions
+        ch_fasta = GENCODE_DOWNLOAD.out.fasta.map { that -> [[id:that.Name], that] }
+        ch_gtf = GENCODE_DOWNLOAD.out.gtf.map { that -> [[id:that.Name], that] }
     } else {
         ch_fasta = Channel.fromPath(params.fasta).map { that -> [[id:that.Name], that] }
         ch_gtf = Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }
     }
 
     if (!file(params.fai).exists() || file(params.fai).isEmpty()){
-        SAMTOOLS_FAIDX(ch_fasta, [[],[]]).fai
+        SAMTOOLS_FAIDX(ch_fasta, [[],[]])
         ch_versions = SAMTOOLS_FAIDX.out.versions
         ch_fai = SAMTOOLS_FAIDX.out.fai
     } else {
@@ -121,39 +119,39 @@ workflow BUILD_REFERENCES {
 //     }
 
 
-    if ((params.fusioncatcher || params.all) &&
-            (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
-            !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
-        if (params.download_refs) {
-            FUSIONCATCHER_DOWNLOAD(params.ensembl_version)
-            ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference}
-        else {
-            FUSIONCATCHER_BUILD(params.ensembl_version)
-            ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference}
-        } else {
-            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
-        }
-
-
-    if ((params.starfusion || params.all) &&
-            (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
-            !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
-        if (params.download_refs) {
-            ch_starfusion_ref = STARFUSION_DOWNLOAD( ch_fasta, ch_gtf ).out.reference }
-        else {
-            ch_starfusion_ref = STARFUSION_BUILD( ch_fasta, ch_gtf ).out.reference }
-    } else {
-        ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }}
-
-
-    if ((params.fusionreport || params.all) &&
-            (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
-            !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
-        if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
-        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd ).out.reference
-    } else {
-        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
-    }
+    // if ((params.fusioncatcher || params.all) &&
+    //         (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
+    //         !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
+    //     if (params.download_refs) {
+    //         FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
+    //         ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference}
+    //     else {
+    //         FUSIONCATCHER_BUILD(params.genome_gencode_version)
+    //         ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference}
+    //     } else {
+    //         ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
+    //     }
+
+
+    // if ((params.starfusion || params.all) &&
+    //         (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
+    //         !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
+    //     if (params.download_refs) {
+    //         ch_starfusion_ref = STARFUSION_DOWNLOAD( ch_fasta, ch_gtf ).out.reference }
+    //     else {
+    //         ch_starfusion_ref = STARFUSION_BUILD( ch_fasta, ch_gtf ).out.reference }
+    // } else {
+    //     ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }}
+
+
+    // if ((params.fusionreport || params.all) &&
+    //         (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
+    //         !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
+    //     if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
+    //     ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd ).out.reference
+    // } else {
+    //     ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
+    // }
 
     emit:
     ch_fasta
@@ -162,16 +160,16 @@ workflow BUILD_REFERENCES {
 
     ch_hgnc_ref
     ch_hgnc_date
-    ch_rrna_interval
-    ch_refflat
-    ch_salmon_index
-    ch_starindex_ref
+    // ch_rrna_interval
+    // ch_refflat
+    // ch_salmon_index
+    // ch_starindex_ref
     // ch_arriba_ref_blacklist
     // ch_arriba_ref_known_fusions
     // ch_arriba_ref_protein_domains
-    ch_fusioncatcher_ref
-    ch_starfusion_ref
-    ch_fusionreport_ref
+    // ch_fusioncatcher_ref
+    // ch_starfusion_ref
+    // ch_fusionreport_ref
     versions        = ch_versions
 }
 
diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
index d7958fecc..177f42d29 100644
--- a/subworkflows/local/qc_workflow.nf
+++ b/subworkflows/local/qc_workflow.nf
@@ -11,7 +11,7 @@ workflow QC_WORKFLOW {
         ch_reads_all
         ch_bam_sorted
         ch_bam_sorted_indexed
-        ch_chrgtf
+        ch_gtf
         ch_refflat
         ch_fasta
         ch_fai
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index de99d8e51..d6a0668a1 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -7,7 +7,7 @@ include { STARFUSION }                                           from '../../mod
 workflow STARFUSION_WORKFLOW {
     take:
         reads
-        ch_chrgtf
+        ch_gtf
         ch_starindex_ref
         ch_fasta
 
@@ -23,7 +23,7 @@ workflow STARFUSION_WORKFLOW {
                 ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
                                         .map { meta, reads, fusions -> [ meta, fusions ] }
             } else {
-                STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_chrgtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
+                STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
                 ch_align = STAR_FOR_STARFUSION.out.bam_sorted
 
diff --git a/subworkflows/local/stringtie_workflow.nf b/subworkflows/local/stringtie_workflow.nf
index 60bd4f38b..d66ec0ee4 100644
--- a/subworkflows/local/stringtie_workflow.nf
+++ b/subworkflows/local/stringtie_workflow.nf
@@ -5,14 +5,14 @@ include { STRINGTIE_MERGE }        from '../../modules/nf-core/stringtie/merge/m
 workflow STRINGTIE_WORKFLOW {
     take:
         bam_sorted
-        ch_chrgtf
+        ch_gtf
 
     main:
         ch_versions = Channel.empty()
         ch_stringtie_gtf = Channel.empty()
 
         if ((params.stringtie || params.all) && !params.fusioninspector_only) {
-            STRINGTIE_STRINGTIE(bam_sorted, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+            STRINGTIE_STRINGTIE(bam_sorted, ch_gtf.map { meta, gtf -> [ gtf ]})
             ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
 
             STRINGTIE_STRINGTIE
@@ -23,7 +23,7 @@ workflow STRINGTIE_WORKFLOW {
             ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
 
 
-            STRINGTIE_MERGE (stringtie_gtf, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+            STRINGTIE_MERGE (stringtie_gtf, ch_gtf.map { meta, gtf -> [ gtf ]})
             ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
             ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
         }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 67dedb4b1..bb24df1f4 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -65,7 +65,7 @@ workflow RNAFUSION {
     // Create references if necessary
     //
 
-    BUILD_REFERENCES(params.genome, params.ensembl_version)
+    BUILD_REFERENCES(params.genome, params.genome_gencode_version)
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
     // // Optional
@@ -177,7 +177,7 @@ workflow RNAFUSION {
 // //Run STAR fusion
 //     STARFUSION_WORKFLOW (
 //         ch_reads_all,
-//         ch_chrgtf,
+//         ch_gtf,
 //         ch_starindex_ref,
 //         ch_fasta
 //     )
@@ -194,7 +194,7 @@ workflow RNAFUSION {
 // //Run stringtie
 //     STRINGTIE_WORKFLOW (
 //         STARFUSION_WORKFLOW.out.ch_bam_sorted,
-//         ch_chrgtf
+//         ch_gtf
 //     )
 //     ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
 
@@ -218,7 +218,7 @@ workflow RNAFUSION {
 //         FUSIONREPORT_WORKFLOW.out.report,
 //         FUSIONREPORT_WORKFLOW.out.csv,
 //         STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-//         ch_chrgtf,
+//         ch_gtf,
 //         ch_arriba_ref_protein_domains,
 //         ch_arriba_ref_cytobands,
 //         ch_hgnc_ref,
@@ -232,7 +232,7 @@ workflow RNAFUSION {
 //         ch_reads_all,
 //         STARFUSION_WORKFLOW.out.ch_bam_sorted,
 //         STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-//         ch_chrgtf,
+//         ch_gtf,
 //         ch_refflat,
 //         ch_fasta,
 //         ch_fai,

From d6ca5b580223b139ce77a95cfcc05912a7155558 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 10:12:27 +0200
Subject: [PATCH 058/887] prettier

---
 nextflow_schema.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 07a31e4c8..60770738f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -515,7 +515,7 @@
     },
     "allOf": [
         {
-            "$ref": "#/$defs/input_output_options"    
+            "$ref": "#/$defs/input_output_options"
         },
         {
             "$ref": "#/$defs/skip_steps"

From e918b8229e9d0c781da5eb7b25f6b663614e98de Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 10:57:00 +0200
Subject: [PATCH 059/887] linitng

---
 nextflow.config        |  18 +---
 workflows/rnafusion.nf | 195 +----------------------------------------
 2 files changed, 3 insertions(+), 210 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 6f2725f83..3ea4afd11 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -125,21 +125,6 @@ params {
 // Load base.config by default for all pipelines
 includeConfig 'conf/base.config'
 
-// Load nf-core custom profiles from different Institutions
-try {
-    includeConfig "${params.custom_config_base}/nfcore_custom.config"
-} catch (Exception e) {
-    System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
-}
-
-// Load nf-core/rnafusion custom profiles from different institutions.
-// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs!
-try {
-    includeConfig "${params.custom_config_base}/pipeline/rnafusion.config"
-} catch (Exception e) {
-    System.err.println("WARNING: Could not load nf-core/config/rnafusion profiles: ${params.custom_config_base}/pipeline/rnafusion.config")
-}
-
 profiles {
     debug {
         dumpHashes              = true
@@ -252,8 +237,7 @@ profiles {
 includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null"
 
 // Load nf-core/rnafusion custom profiles from different institutions.
-// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
-// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rnafusion.config" : "/dev/null"
+includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rnafusion.config" : "/dev/null"
 
 // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile
 // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index c3831c47d..53c51b00d 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -9,58 +9,7 @@ include { paramsSummaryMap       } from 'plugin/nf-schema'
 include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
-include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation'
-
-ch_chrgtf = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
-ch_rrna_interval = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
-ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
-ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
-ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
-ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
-ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
-ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
-
 
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT LOCAL MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
-//
-
-include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
-include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
-include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
-include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
-include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow'
-include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
-include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
-include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
-include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT NF-CORE MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// MODULE: Installed directly from nf-core/modules
-//
-include { CAT_FASTQ                   } from '../modules/nf-core/cat/fastq/main'
-include { SALMON_QUANT                } from '../modules/nf-core/salmon/quant/main'
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -71,148 +20,18 @@ workflow RNAFUSION {
 
     take:
     ch_samplesheet // channel: samplesheet read in from --input
-    
     main:
 
     ch_versions = Channel.empty()
     ch_multiqc_files = Channel.empty()
-    //
-    // Create channel from input file provided through params.input
-    //
-    Channel
-        .fromSamplesheet("input")
-        .map {
-            meta, fastq_1, fastq_2, strandedness ->
-                if (!fastq_2) {
-                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
-                } else {
-                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
-                }
-        }
-        .groupTuple()
-        .map {
-            validateInputSamplesheet(it)
-        }
-        .branch {
-            meta, fastqs ->
-                single  : fastqs.size() == 1
-                    return [ meta, fastqs.flatten() ]
-                multiple: fastqs.size() > 1
-                    return [ meta, fastqs.flatten() ]
-        }
-        .set { ch_fastq }
-
-    //
-    // MODULE: Concatenate FastQ files from same sample if required
-    //
-    CAT_FASTQ (
-        ch_fastq.multiple
-    )
-    .reads
-    .mix(ch_fastq.single)
-    .set { ch_cat_fastq }
-    ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
-
-
     //
     // MODULE: Run FastQC
     //
     FASTQC (
-        ch_cat_fastq
+        ch_samplesheet
     )
     ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    ch_versions = ch_versions.mix(FASTQC.out.versions)
-
-    TRIM_WORKFLOW (
-        ch_cat_fastq
-    )
-    ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
-    ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
-    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
-
-
-    SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
-
-
-    //
-    // SUBWORKFLOW:  Run STAR alignment and Arriba
-    //
-    ARRIBA_WORKFLOW (
-        ch_reads_all,
-        ch_gtf,
-        ch_fasta,
-        ch_starindex_ensembl_ref,
-        ch_arriba_ref_blacklist,
-        ch_arriba_ref_known_fusions,
-        ch_arriba_ref_protein_domains
-    )
-    ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
-
-
-//Run STAR fusion
-    STARFUSION_WORKFLOW (
-        ch_reads_all,
-        ch_chrgtf,
-        ch_starindex_ref,
-        ch_fasta
-    )
-    ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
-
-
-//Run fusioncatcher
-    FUSIONCATCHER_WORKFLOW (
-        ch_reads_fusioncatcher
-    )
-    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
-
-
-//Run stringtie
-    STRINGTIE_WORKFLOW (
-        STARFUSION_WORKFLOW.out.ch_bam_sorted,
-        ch_chrgtf
-    )
-    ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
-
-
-    //Run fusion-report
-    FUSIONREPORT_WORKFLOW (
-        ch_reads_all,
-        ch_fusionreport_ref,
-        ARRIBA_WORKFLOW.out.fusions,
-        STARFUSION_WORKFLOW.out.fusions,
-        FUSIONCATCHER_WORKFLOW.out.fusions
-    )
-    ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
-
-
-    //Run fusionInpector
-    FUSIONINSPECTOR_WORKFLOW (
-        ch_reads_all,
-        FUSIONREPORT_WORKFLOW.out.fusion_list,
-        FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
-        FUSIONREPORT_WORKFLOW.out.report,
-        FUSIONREPORT_WORKFLOW.out.csv,
-        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-        ch_chrgtf,
-        ch_arriba_ref_protein_domains,
-        ch_arriba_ref_cytobands,
-        ch_hgnc_ref,
-        ch_hgnc_date
-    )
-    ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
-
-
-    //QC
-    QC_WORKFLOW (
-        ch_reads_all,
-        STARFUSION_WORKFLOW.out.ch_bam_sorted,
-        ch_chrgtf,
-        ch_refflat,
-        ch_fasta,
-        ch_fai,
-        ch_rrna_interval
-    )
-    ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
+    ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
     //
     // Collate and save software versions
@@ -256,16 +75,6 @@ workflow RNAFUSION {
             sort: true
         )
     )
-    ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
 
     MULTIQC (
         ch_multiqc_files.collect(),

From 90fab1933ac9a0047f84d8ddfd9cb4dff65cd481 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 11:57:50 +0200
Subject: [PATCH 060/887] fix input checks

---
 assets/schema_input.json | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index 96879f358..9747d48aa 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -29,9 +29,7 @@
             },
             "strandedness": {
                 "type": "string",
-                "format": "string",
-                "exists": true,
-                "pattern": "forward|reverse|unstranded|unknown",
+                "enum": ["forward", "reverse", "unstranded", "unknown"],
                 "errorMessage": "Strandedness has to be forward, reverse, unstranded or unknown"
             }
         },

From 82da72980c223cffb750b92befab1ae3dde95303 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 12:06:33 +0200
Subject: [PATCH 061/887] add igenomes to linting exclusion

---
 .nf-core.yml                 | 2 ++
 conf/igenomes_ignored.config | 9 ---------
 2 files changed, 2 insertions(+), 9 deletions(-)
 delete mode 100644 conf/igenomes_ignored.config

diff --git a/.nf-core.yml b/.nf-core.yml
index 097a52b0e..49964714b 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -3,6 +3,8 @@ lint:
   files_unchanged:
     - .github/CONTRIBUTING.md
     - .github/PULL_REQUEST_TEMPLATE.md
+    - conf/igenomes.config
+    - conf/igenomes_ignored.config
 nf_core_version: 3.0.2
 org_path: null
 repository_type: pipeline
diff --git a/conf/igenomes_ignored.config b/conf/igenomes_ignored.config
deleted file mode 100644
index b4034d824..000000000
--- a/conf/igenomes_ignored.config
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Nextflow config file for iGenomes paths
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Empty genomes dictionary to use when igenomes is ignored.
-----------------------------------------------------------------------------------------
-*/
-
-params.genomes = [:]

From 4b93703cc841ae65497bc9db20d666d4ca4bc4cf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 12:13:46 +0200
Subject: [PATCH 062/887] remove exist false for input as logic changed

---
 nextflow_schema.json | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 60770738f..470b1300b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -32,7 +32,6 @@
                 "input": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": false,
                     "schema": "assets/schema_input.json",
                     "mimetype": "text/csv",
                     "pattern": "^\\S+\\.csv$",

From 046c74d6e9ea9c246c61d0fd057e470810cf8854 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 12:47:38 +0200
Subject: [PATCH 063/887] declare samplesheet as input

---
 main.nf | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/main.nf b/main.nf
index d6d7364c5..2508e74bb 100644
--- a/main.nf
+++ b/main.nf
@@ -42,6 +42,8 @@ include { RNAFUSION               } from './workflows/rnafusion'
 // WORKFLOW: Run main analysis pipeline depending on type of input
 //
 workflow NFCORE_RNAFUSION {
+    take:
+    samplesheet
 
     main:
 

From e8949e00f5d215e5bf8cefc784d4ff47ac30bac2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Oct 2024 17:30:20 +0200
Subject: [PATCH 064/887] adapt samplesheet

---
 main.nf                                                    | 1 -
 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 6 +++---
 2 files changed, 3 insertions(+), 4 deletions(-)

diff --git a/main.nf b/main.nf
index 2508e74bb..ced04ca91 100644
--- a/main.nf
+++ b/main.nf
@@ -54,7 +54,6 @@ workflow NFCORE_RNAFUSION {
     if (params.build_references) {
         BUILD_REFERENCES ()
     } else {
-        ch_samplesheet = Channel.value(file(params.input, checkIfExists: true))
         RNAFUSION(samplesheet)
     }
 
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 72a6c82a7..ae62c8d4c 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -77,11 +77,11 @@ workflow PIPELINE_INITIALISATION {
     Channel
         .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json"))
         .map {
-            meta, fastq_1, fastq_2 ->
+            meta, fastq_1, fastq_2, strandedness ->
                 if (!fastq_2) {
-                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
+                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ], strandedness ]
                 } else {
-                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
+                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ], strandedness ]
                 }
         }
         .groupTuple()

From 94fb2461b6ea8ad9763bfa16865ace07f95272b1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 09:25:00 +0200
Subject: [PATCH 065/887] change output dir to avoid overwriting

---
 .github/workflows/ci.yml | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index f84c77191..a729acf88 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -87,12 +87,12 @@ jobs:
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} \
           -stub --build_references \
-          --outdir /home/runner/work/rnafusion/rnafusion/results --all \
-          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references \
+          --outdir ./results --all \
+          --genomes_base ./results/references \
           --cosmic_username ${{ secrets.COSMIC_USERNAME }} --cosmic_passwd ${{ secrets.COSMIC_PASSWD }}
 
       - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile -profile ${{ matrix.test_name }},${{ matrix.profile }} -stub \
-          --outdir /home/runner/work/rnafusion/rnafusion/results --all ${{ matrix.trim_parameters }} \
-          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
+          --outdir ./results --all ${{ matrix.trim_parameters }} \
+          --genomes_base ./results/references

From 4e029f81c2bc4e364be6d464bfc91241b72dc108 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 09:33:44 +0200
Subject: [PATCH 066/887] correct error in output of version in hgcn

---
 modules/local/hgnc/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 1b3808f6d..27c4321f7 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -23,7 +23,7 @@ process HGNC_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
     END_VERSIONS
     """
 
@@ -34,7 +34,7 @@ process HGNC_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
     END_VERSIONS
     """
 

From 8d7f7a9a75e555a1e1edeeee10a83a3641ebb450 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 09:34:34 +0200
Subject: [PATCH 067/887] revert change of dir

---
 .github/workflows/ci.yml | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index a729acf88..f84c77191 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -87,12 +87,12 @@ jobs:
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} \
           -stub --build_references \
-          --outdir ./results --all \
-          --genomes_base ./results/references \
+          --outdir /home/runner/work/rnafusion/rnafusion/results --all \
+          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references \
           --cosmic_username ${{ secrets.COSMIC_USERNAME }} --cosmic_passwd ${{ secrets.COSMIC_PASSWD }}
 
       - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile -profile ${{ matrix.test_name }},${{ matrix.profile }} -stub \
-          --outdir ./results --all ${{ matrix.trim_parameters }} \
-          --genomes_base ./results/references
+          --outdir /home/runner/work/rnafusion/rnafusion/results --all ${{ matrix.trim_parameters }} \
+          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references

From 33e394c33c5eafc8b1a8ad5e832e33b349baf863 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 09:43:24 +0200
Subject: [PATCH 068/887] correct for ensembl too

---
 modules/local/ensembl/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 8297e2901..4715690c6 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -31,7 +31,7 @@ process ENSEMBL_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
         gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
 
     END_VERSIONS
@@ -45,7 +45,7 @@ process ENSEMBL_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
         gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
     END_VERSIONS
     """

From 93254d2523f8e4cc5db9d8c62d49110fb974e3ff Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 09:52:06 +0200
Subject: [PATCH 069/887] remove check max

---
 conf/modules.config | 18 +++++++++---------
 1 file changed, 9 insertions(+), 9 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 25169abb3..699720400 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -304,9 +304,9 @@ process {
 
     withName: 'STAR_GENOMEGENERATE' {
         ext.args = "--sjdbOverhang ${params.read_length - 1}"
-        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 2.d  * task.attempt, 'time'    ) }
+        cpus   = { 24    * task.attempt }
+        memory = { 100.GB * task.attempt }
+        time   = { 2.d  * task.attempt }
         publishDir = [
             path: { "${params.genomes_base}" },
             mode: params.publish_dir_mode,
@@ -315,9 +315,9 @@ process {
     }
 
     withName: 'STARFUSION_BUILD' {
-        cpus   = { check_max( 24    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 100.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 2.d  * task.attempt, 'time'    ) }
+        cpus   = { 24    * task.attempt }
+        memory = { 100.GB * task.attempt }
+        time   = { 2.d  * task.attempt }
         publishDir = [
             path: { "${params.genomes_base}/starfusion" },
             mode: params.publish_dir_mode,
@@ -326,9 +326,9 @@ process {
     }
 
     withName: 'STARFUSION_DOWNLOAD' {
-        cpus   = { check_max( 2    * task.attempt, 'cpus'    ) }
-        memory = { check_max( 24.GB * task.attempt, 'memory'  ) }
-        time   = { check_max( 6.h  * task.attempt, 'time'    ) }
+        cpus   = { 2    * task.attempt }
+        memory = { 24.GB * task.attempt }
+        time   = { 6.h  * task.attempt }
         publishDir = [
             path: { "${params.genomes_base}/starfusion" },
             mode: params.publish_dir_mode,

From 533a7daca15138b107e9c2e82225430f84e1599e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 10:01:28 +0200
Subject: [PATCH 070/887] remove duplicate profile

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index f84c77191..e1028ed8e 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -93,6 +93,6 @@ jobs:
 
       - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile -profile ${{ matrix.test_name }},${{ matrix.profile }} -stub \
+          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} -stub \
           --outdir /home/runner/work/rnafusion/rnafusion/results --all ${{ matrix.trim_parameters }} \
           --genomes_base /home/runner/work/rnafusion/rnafusion/results/references

From b3a67f5ad7bd551af5bb252bf2087a4389920aed Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 11:56:30 +0200
Subject: [PATCH 071/887] backtrack rnafusion main

---
 workflows/rnafusion.nf | 131 ++++++++++++++++++++++++++++++++++++++++-
 1 file changed, 129 insertions(+), 2 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 53c51b00d..dd1dedb40 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -3,13 +3,44 @@
     IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
+include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
+include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
+include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
+include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
+include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow'
+include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
+include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
+include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
+include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
+include { CAT_FASTQ              } from '../modules/nf-core/cat/fastq/main'
 include { FASTQC                 } from '../modules/nf-core/fastqc/main'
 include { MULTIQC                } from '../modules/nf-core/multiqc/main'
+include { SALMON_QUANT           } from '../modules/nf-core/salmon/quant/main'
 include { paramsSummaryMap       } from 'plugin/nf-schema'
 include { paramsSummaryMultiqc   } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 
+
+ch_chrgtf = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect()
+ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
+ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
+ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
+ch_rrna_interval = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
+ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
+ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
+ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
+ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
+ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
+ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
+ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
+ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
+ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
+ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
+ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
+ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
+
+
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -33,6 +64,100 @@ workflow RNAFUSION {
     ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
     ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
+
+
+    TRIM_WORKFLOW (
+        ch_samplesheet
+    )
+    ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
+    ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
+    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+
+
+    SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+
+
+    //
+    // SUBWORKFLOW:  Run STAR alignment and Arriba
+    //
+    ARRIBA_WORKFLOW (
+        ch_reads_all,
+        ch_gtf,
+        ch_fasta,
+        ch_starindex_ensembl_ref,
+        ch_arriba_ref_blacklist,
+        ch_arriba_ref_known_fusions,
+        ch_arriba_ref_protein_domains
+    )
+    ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
+
+
+    //Run STAR fusion
+    STARFUSION_WORKFLOW (
+        ch_reads_all,
+        ch_chrgtf,
+        ch_starindex_ref,
+        ch_fasta
+    )
+    ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+
+
+    //Run fusioncatcher
+    FUSIONCATCHER_WORKFLOW (
+        ch_reads_fusioncatcher
+    )
+    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+
+
+    //Run stringtie
+    STRINGTIE_WORKFLOW (
+        STARFUSION_WORKFLOW.out.ch_bam_sorted,
+        ch_chrgtf
+    )
+    ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
+
+
+    //Run fusion-report
+    FUSIONREPORT_WORKFLOW (
+        ch_reads_all,
+        ch_fusionreport_ref,
+        ARRIBA_WORKFLOW.out.fusions,
+        STARFUSION_WORKFLOW.out.fusions,
+        FUSIONCATCHER_WORKFLOW.out.fusions
+    )
+    ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
+
+
+    //Run fusionInpector
+    FUSIONINSPECTOR_WORKFLOW (
+        ch_reads_all,
+        FUSIONREPORT_WORKFLOW.out.fusion_list,
+        FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
+        FUSIONREPORT_WORKFLOW.out.report,
+        FUSIONREPORT_WORKFLOW.out.csv,
+        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+        ch_chrgtf,
+        ch_arriba_ref_protein_domains,
+        ch_arriba_ref_cytobands,
+        ch_hgnc_ref,
+        ch_hgnc_date
+    )
+    ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
+
+
+    //QC
+    QC_WORKFLOW (
+        ch_reads_all,
+        STARFUSION_WORKFLOW.out.ch_bam_sorted,
+        ch_chrgtf,
+        ch_refflat,
+        ch_fasta,
+        ch_fai,
+        ch_rrna_interval
+    )
+    ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
+
+
     //
     // Collate and save software versions
     //
@@ -44,7 +169,6 @@ workflow RNAFUSION {
             newLine: true
         ).set { ch_collated_versions }
 
-
     //
     // MODULE: MultiQC
     //
@@ -85,7 +209,10 @@ workflow RNAFUSION {
         []
     )
 
-    emit:multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+
+
+    emit:
+    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
     versions       = ch_versions                 // channel: [ path(versions.yml) ]
 
 }

From 6773f5bac62303753976b3949ad90b3e2b227f07 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 14:35:13 +0200
Subject: [PATCH 072/887] update containers to nf-core and quay registry

---
 modules/local/ensembl/main.nf                | 2 +-
 modules/local/fusioncatcher/detect/main.nf   | 2 +-
 modules/local/fusioncatcher/download/main.nf | 2 +-
 modules/local/fusioninspector/main.nf        | 2 +-
 modules/local/fusionreport/detect/main.nf    | 2 +-
 modules/local/fusionreport/download/main.nf  | 2 +-
 modules/local/hgnc/main.nf                   | 2 +-
 modules/local/rrnatranscripts/main.nf        | 2 +-
 modules/local/starfusion/build/main.nf       | 2 +-
 modules/local/starfusion/detect/main.nf      | 2 +-
 modules/local/starfusion/download/main.nf    | 2 +-
 modules/local/vcf_collect/main.nf            | 2 +-
 12 files changed, 12 insertions(+), 12 deletions(-)

diff --git a/modules/local/ensembl/main.nf b/modules/local/ensembl/main.nf
index 4715690c6..8f708fc26 100644
--- a/modules/local/ensembl/main.nf
+++ b/modules/local/ensembl/main.nf
@@ -5,7 +5,7 @@ process ENSEMBL_DOWNLOAD {
     conda "bioconda::gnu-wget=1.18"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
-        'quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
+        'biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
 
     input:
     val ensembl_version
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 2977d6686..30e641db2 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER {
     label 'process_high'
 
     conda "bioconda::fusioncatcher=1.33"
-    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
+    container "nf-core/fusioncatcher:1.33"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 156e70b65..0f9178c84 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER_DOWNLOAD {
     label 'process_medium'
 
     conda "bioconda::fusioncatcher=1.33"
-    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
+    container "nf-core/fusioncatcher:1.33"
 
     output:
     path "*"                , emit: reference
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 2f99c7111..1b04ce2c8 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -3,7 +3,7 @@ process FUSIONINSPECTOR {
     label 'process_high'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container 'docker.io/trinityctat/starfusion:1.13.0'
+    container "nf-core/starfusion:1.13.0"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 8024d8f8e..386bb3ae4 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -3,7 +3,7 @@ process FUSIONREPORT {
     label 'process_medium'
 
     conda "bioconda::star=2.7.9a"
-    container "docker.io/clinicalgenomics/fusion-report:2.1.8"
+    container "nf-core/fusion-report:2.1.8"
 
 
     input:
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index ac288ade9..3d08360ca 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -3,7 +3,7 @@ process FUSIONREPORT_DOWNLOAD {
     label 'process_medium'
 
     conda "bioconda::star=2.7.9a"
-    container "docker.io/clinicalgenomics/fusion-report:2.1.8"
+    container "nf-core/fusion-report:2.1.8"
 
     input:
     val(username)
diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 27c4321f7..865c59d24 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -5,7 +5,7 @@ process HGNC_DOWNLOAD {
     conda "bioconda::gnu-wget=1.18"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
-        'quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
+        'biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
 
     input:
 
diff --git a/modules/local/rrnatranscripts/main.nf b/modules/local/rrnatranscripts/main.nf
index c31de7076..fb4ec2d62 100644
--- a/modules/local/rrnatranscripts/main.nf
+++ b/modules/local/rrnatranscripts/main.nf
@@ -6,7 +6,7 @@ process RRNA_TRANSCRIPTS {
 
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/python:3.12' :
-        'quay.io/biocontainers/python:3.12' }"
+        'biocontainers/python:3.12' }"
 
     input:
         tuple val(meta), path(gtf)
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index a768cc5c9..14b0eaf7c 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -2,7 +2,7 @@ process STARFUSION_BUILD {
     tag 'star-fusion'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container "docker.io/trinityctat/starfusion:1.13.0"
+    container "nf-core/starfusion:1.13.0"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 9a46cc00d..cc13e4f4c 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -3,7 +3,7 @@ process STARFUSION {
     label 'process_high'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container 'docker.io/trinityctat/starfusion:1.13.0'
+    container "nf-core/starfusion:1.13.0"
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 221d12dd9..5eb5bdef7 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -2,7 +2,7 @@ process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container 'docker.io/trinityctat/starfusion:1.13.0'
+    container "nf-core/starfusion:1.13.0"
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference
diff --git a/modules/local/vcf_collect/main.nf b/modules/local/vcf_collect/main.nf
index 3ede7936c..3a1bf2217 100644
--- a/modules/local/vcf_collect/main.nf
+++ b/modules/local/vcf_collect/main.nf
@@ -5,7 +5,7 @@ process VCF_COLLECT {
     conda "conda-forge::pandas=1.5.2"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/pandas:1.5.2' :
-        'quay.io/biocontainers/pandas:1.5.2' }"
+        'biocontainers/pandas:1.5.2' }"
 
     input:
     tuple val(meta), path(fusioninspector_tsv), path(fusioninspector_gtf_tsv), path(fusionreport_report), path(fusionreport_csv)

From 1d6304012c4ebf8a07ecd5ee929aa44e21b55153 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 15:00:58 +0200
Subject: [PATCH 073/887] remove quay but leave the docker.io repo

---
 modules/local/fusioncatcher/detect/main.nf   | 2 +-
 modules/local/fusioncatcher/download/main.nf | 2 +-
 modules/local/fusioninspector/main.nf        | 2 +-
 modules/local/fusionreport/detect/main.nf    | 2 +-
 modules/local/fusionreport/download/main.nf  | 2 +-
 modules/local/starfusion/build/main.nf       | 2 +-
 modules/local/starfusion/detect/main.nf      | 2 +-
 modules/local/starfusion/download/main.nf    | 2 +-
 8 files changed, 8 insertions(+), 8 deletions(-)

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 30e641db2..2977d6686 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER {
     label 'process_high'
 
     conda "bioconda::fusioncatcher=1.33"
-    container "nf-core/fusioncatcher:1.33"
+    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 0f9178c84..156e70b65 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER_DOWNLOAD {
     label 'process_medium'
 
     conda "bioconda::fusioncatcher=1.33"
-    container "nf-core/fusioncatcher:1.33"
+    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
 
     output:
     path "*"                , emit: reference
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 1b04ce2c8..2f99c7111 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -3,7 +3,7 @@ process FUSIONINSPECTOR {
     label 'process_high'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container "nf-core/starfusion:1.13.0"
+    container 'docker.io/trinityctat/starfusion:1.13.0'
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 386bb3ae4..8024d8f8e 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -3,7 +3,7 @@ process FUSIONREPORT {
     label 'process_medium'
 
     conda "bioconda::star=2.7.9a"
-    container "nf-core/fusion-report:2.1.8"
+    container "docker.io/clinicalgenomics/fusion-report:2.1.8"
 
 
     input:
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 3d08360ca..ac288ade9 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -3,7 +3,7 @@ process FUSIONREPORT_DOWNLOAD {
     label 'process_medium'
 
     conda "bioconda::star=2.7.9a"
-    container "nf-core/fusion-report:2.1.8"
+    container "docker.io/clinicalgenomics/fusion-report:2.1.8"
 
     input:
     val(username)
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 14b0eaf7c..a768cc5c9 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -2,7 +2,7 @@ process STARFUSION_BUILD {
     tag 'star-fusion'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container "nf-core/starfusion:1.13.0"
+    container "docker.io/trinityctat/starfusion:1.13.0"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index cc13e4f4c..9a46cc00d 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -3,7 +3,7 @@ process STARFUSION {
     label 'process_high'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container "nf-core/starfusion:1.13.0"
+    container 'docker.io/trinityctat/starfusion:1.13.0'
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 5eb5bdef7..221d12dd9 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -2,7 +2,7 @@ process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
     conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container "nf-core/starfusion:1.13.0"
+    container 'docker.io/trinityctat/starfusion:1.13.0'
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference

From 898306eb79717f81f2205018ce865a48cdb35a2c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 15:25:00 +0200
Subject: [PATCH 074/887] add files to multiqc

---
 workflows/rnafusion.nf | 10 ++++++++++
 1 file changed, 10 insertions(+)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index dd1dedb40..b4e4517b6 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -199,6 +199,16 @@ workflow RNAFUSION {
             sort: true
         )
     )
+    ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
 
     MULTIQC (
         ch_multiqc_files.collect(),

From b1891c56af96984de3e1174aee76612d4f70a986 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Oct 2024 15:27:22 +0200
Subject: [PATCH 075/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fe350b46c..4d4e4e5c6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -14,6 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Changed
 
 - Updated modules and migrated non-specific modules to nf-core/modules [#484](https://github.com/nf-core/rnafusion/pull/484)
+- Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 
 ### Fixed
 

From 0b1a0672a59a31918c9fddb54fdffc883768800d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Nov 2024 10:03:44 +0100
Subject: [PATCH 076/887] syntax

---
 conf/modules.config                        |  2 +-
 modules/local/arriba/visualisation/main.nf |  8 ++++----
 modules/local/hgnc/main.nf                 |  2 --
 modules/nf-core/arriba/download/main.nf    |  2 --
 modules/nf-core/arriba/main.nf             | 20 ++++++++++----------
 subworkflows/local/build_references.nf     | 18 +++++++++---------
 6 files changed, 24 insertions(+), 28 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index af8324983..232efdd16 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -63,7 +63,7 @@ process {
 
     withName: 'FASTQC' {
         ext.args = '--quiet'
-        ext.when = { !params.skip_qc and not !params.build_references }
+        ext.when = { !params.skip_qc && !params.build_references }
         publishDir = [
             path: { "${params.outdir}/fastqc" },
             mode: params.publish_dir_mode,
diff --git a/modules/local/arriba/visualisation/main.nf b/modules/local/arriba/visualisation/main.nf
index cc1201191..f1aa097b6 100644
--- a/modules/local/arriba/visualisation/main.nf
+++ b/modules/local/arriba/visualisation/main.nf
@@ -22,17 +22,17 @@ process ARRIBA_VISUALISATION {
 
     script:
     def args = task.ext.args ?: ''
-    def cytobands = cytobands ? " --cytobands=$cytobands" : ""
+    def arg_cytobands = cytobands ? " --cytobands=$cytobands" : ""
+    def arg_protein_domains = protein_domains ? "--proteinDomains=$protein_domains" : ""
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def protein_domains = protein_domains ? "--proteinDomains=$protein_domains" : ""
     """
     draw_fusions.R \\
         --fusions=$fusions \\
         --alignments=$bam \\
         --output=${prefix}.pdf \\
         --annotation=${gtf} \\
-        $cytobands \\
-        $protein_domains \\
+        $arg_cytobands \\
+        $arg_protein_domains \\
         $args
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 6a0556314..5a250f0f5 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -7,8 +7,6 @@ process HGNC_DOWNLOAD {
         'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
         'quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
 
-    input:
-
     output:
     path "hgnc_complete_set.txt"        , emit: hgnc_ref
     path "HGNC-DB-timestamp.txt"        , emit: hgnc_date
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index 96a4e3362..7f11898bd 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -7,8 +7,6 @@ process ARRIBA_DOWNLOAD {
         'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
         'biocontainers/arriba:2.4.0--h0033a41_2' }"
 
-    input:
-
     output:
     path "*"              , emit: reference
     path "versions.yml"           , emit: versions
diff --git a/modules/nf-core/arriba/main.nf b/modules/nf-core/arriba/main.nf
index 2537d05b8..66809530d 100644
--- a/modules/nf-core/arriba/main.nf
+++ b/modules/nf-core/arriba/main.nf
@@ -28,11 +28,11 @@ process ARRIBA {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
-    def known_fusions = known_fusions ? "-k $known_fusions" : ""
-    def structural_variants = structural_variants ? "-d $structual_variants" : ""
-    def tags = tags ? "-t $tags" : ""
-    def protein_domains = protein_domains ? "-p $protein_domains" : ""
+    def arg_blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
+    def arg_known_fusions = known_fusions ? "-k $known_fusions" : ""
+    def arg_structural_variants = structural_variants ? "-d $structural_variants" : ""
+    def arg_tags = tags ? "-t $tags" : ""
+    def arg_protein_domains = protein_domains ? "-p $protein_domains" : ""
 
     """
     arriba \\
@@ -41,11 +41,11 @@ process ARRIBA {
         -g $gtf \\
         -o ${prefix}.fusions.tsv \\
         -O ${prefix}.fusions.discarded.tsv \\
-        $blacklist \\
-        $known_fusions \\
-        $structural_variants \\
-        $tags \\
-        $protein_domains \\
+        $arg_blacklist \\
+        $arg_known_fusions \\
+        $arg_structural_variants \\
+        $arg_tags \\
+        $arg_protein_domains \\
         $args
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 8017f6346..1d45e74ca 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -133,15 +133,15 @@ workflow BUILD_REFERENCES {
     //     }
 
 
-    // if ((params.starfusion || params.all) &&
-    //         (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
-    //         !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
-    //     if (params.download_refs) {
-    //         ch_starfusion_ref = STARFUSION_DOWNLOAD( ch_fasta, ch_gtf ).out.reference }
-    //     else {
-    //         ch_starfusion_ref = STARFUSION_BUILD( ch_fasta, ch_gtf ).out.reference }
-    // } else {
-    //     ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }}
+    if ((params.starfusion || params.all) &&
+            (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
+            !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
+        if (params.download_refs) {
+            ch_starfusion_ref = STARFUSION_DOWNLOAD( ch_fasta, ch_gtf ).out.reference }
+        else {
+            ch_starfusion_ref = STARFUSION_BUILD( ch_fasta, ch_gtf ).out.reference }
+    } else {
+        ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }}
 
 
     // if ((params.fusionreport || params.all) &&

From f134b84c8a5977cee935aa5396eef504f6c278fd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Nov 2024 10:35:46 +0100
Subject: [PATCH 077/887] merging

---
 conf/modules.config                  | 14 --------------
 modules/nf-core/salmon/quant/main.nf |  2 +-
 workflows/rnafusion.nf               |  1 +
 3 files changed, 2 insertions(+), 15 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 7afa2817e..113c406ce 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -127,7 +127,6 @@ process {
         ]
     }
 
-<<<<<<< HEAD
     withName: 'GFFREAD' {
         ext.args = { '-w' }
         publishDir = [
@@ -145,8 +144,6 @@ process {
         ]
     }
 
-=======
->>>>>>> 22d0e803ca87786359cf582bfb4ac8114adde8fd
     withName: 'HGNC_DOWNLOAD' {
         publishDir = [
             path: { "${params.genomes_base}/hgnc" },
@@ -369,20 +366,9 @@ process {
         ]
     }
 
-<<<<<<< HEAD
     withName: 'TRIM_WORKFLOW:*' {
         ext.when = { !params.build_references }
 
-=======
-    withName: 'UCSC_GTFTOGENEPRED' {
-        ext.args   = "-genePredExt -geneNameAsName2"
-        publishDir = [
-            path: { "${params.genomes_base}/ensembl" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-        ]
->>>>>>> 22d0e803ca87786359cf582bfb4ac8114adde8fd
-    }
 
     withName: 'VCF_COLLECT' {
         ext.when = {!params.fusioninspector_only}
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index 6c528b240..6d624e751 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -28,7 +28,7 @@ process SALMON_QUANT {
     def args = task.ext.args   ?: ''
     prefix   = task.ext.prefix ?: "${meta.id}"
 
-    def reference   = "--index $index"
+    def reference = "--index $index"
     def reads1 = [], reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def input_reads = meta.single_end ? "-r ${reads1.join(" ")}" : "-1 ${reads1.join(" ")} -2 ${reads2.join(" ")}"
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 9b615d403..8f7f8ae8a 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -39,6 +39,7 @@ include { CAT_FASTQ              } from '../modules/nf-core/cat/fastq/main'
 include { FASTQC                 } from '../modules/nf-core/fastqc/main'
 include { MULTIQC                } from '../modules/nf-core/multiqc/main'
 include { SALMON_QUANT           } from '../modules/nf-core/salmon/quant/main'
+
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW

From 7a5a91700ad963723a0a837d7f8cdb212947e8fd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 13 Nov 2024 14:20:16 +0100
Subject: [PATCH 078/887] cleanup

---
 conf/modules.config                           |   2 +-
 main.nf                                       |   7 +-
 modules.json                                  | 142 ++++++++++++------
 modules/local/fusioncatcher/build/main.nf     |   1 -
 modules/local/fusioncatcher/download/main.nf  |  20 +--
 modules/local/gencode_download/main.nf        |   4 +-
 modules/local/get_rrna_transcripts.nf         |  43 ++++++
 modules/nf-core/arriba/arriba/environment.yml |   3 +
 modules/nf-core/arriba/arriba/main.nf         |  29 ++--
 modules/nf-core/arriba/arriba/meta.yml        |  39 +----
 .../nf-core/arriba/arriba/tests/main.nf.test  | 107 +++++++++++++
 .../arriba/arriba/tests/main.nf.test.snap     |  55 +++++++
 .../arriba/arriba/tests/nextflow.config       |   8 +
 modules/nf-core/arriba/download/main.nf       |  13 +-
 modules/nf-core/arriba/download/meta.yml      |  32 +++-
 .../arriba/download/tests/main.nf.test        |  29 +++-
 .../arriba/download/tests/main.nf.test.snap   |  62 ++++----
 .../nf-core/arriba/download/tests/tags.yml    |   2 +
 modules/nf-core/gunzip/environment.yml        |   7 -
 modules/nf-core/gunzip/main.nf                |  55 -------
 modules/nf-core/gunzip/meta.yml               |  47 ------
 modules/nf-core/gunzip/tests/main.nf.test     | 121 ---------------
 .../nf-core/gunzip/tests/main.nf.test.snap    | 134 -----------------
 modules/nf-core/gunzip/tests/nextflow.config  |   5 -
 modules/nf-core/gunzip/tests/tags.yml         |   2 -
 modules/nf-core/salmon/quant/environment.yml  |   2 +-
 modules/nf-core/salmon/quant/main.nf          |   6 +-
 .../salmon/quant/tests/main.nf.test.snap      |  64 ++++----
 nextflow.config                               |   3 +-
 nextflow_schema.json                          |  19 ++-
 subworkflows/local/build_references.nf        | 102 +++++++------
 workflows/rnafusion.nf                        |  52 ++-----
 32 files changed, 564 insertions(+), 653 deletions(-)
 create mode 100644 modules/local/get_rrna_transcripts.nf
 create mode 100644 modules/nf-core/arriba/arriba/tests/main.nf.test
 create mode 100644 modules/nf-core/arriba/arriba/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/arriba/arriba/tests/nextflow.config
 create mode 100644 modules/nf-core/arriba/download/tests/tags.yml
 delete mode 100644 modules/nf-core/gunzip/environment.yml
 delete mode 100644 modules/nf-core/gunzip/main.nf
 delete mode 100644 modules/nf-core/gunzip/meta.yml
 delete mode 100644 modules/nf-core/gunzip/tests/main.nf.test
 delete mode 100644 modules/nf-core/gunzip/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/gunzip/tests/nextflow.config
 delete mode 100644 modules/nf-core/gunzip/tests/tags.yml

diff --git a/conf/modules.config b/conf/modules.config
index 113c406ce..123e02b08 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -368,7 +368,7 @@ process {
 
     withName: 'TRIM_WORKFLOW:*' {
         ext.when = { !params.build_references }
-
+    }
 
     withName: 'VCF_COLLECT' {
         ext.when = {!params.fusioninspector_only}
diff --git a/main.nf b/main.nf
index ce7352f13..09216f56b 100644
--- a/main.nf
+++ b/main.nf
@@ -41,6 +41,8 @@ include { RNAFUSION               } from './workflows/rnafusion'
 // WORKFLOW: Run main analysis pipeline depending on type of input
 //
 workflow NFCORE_RNAFUSION {
+    take:
+    samplesheet
 
     main:
 
@@ -48,8 +50,7 @@ workflow NFCORE_RNAFUSION {
     // WORKFLOW: Run pipeline
     //
 
-    ch_samplesheet = Channel.value(file(params.input, checkIfExists: true))
-    RNAFUSION(ch_samplesheet)
+    RNAFUSION(samplesheet)
 }
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -75,7 +76,7 @@ workflow {
     //
     // WORKFLOW: Run main workflow
     //
-    NFCORE_RNAFUSION ()
+    NFCORE_RNAFUSION (PIPELINE_INITIALISATION.out.samplesheet)
 
     //
     // SUBWORKFLOW: Run completion tasks
diff --git a/modules.json b/modules.json
index e71954a61..7e5065489 100644
--- a/modules.json
+++ b/modules.json
@@ -8,152 +8,198 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "git_sha": "7741dfc830e77a8ead2fcb50b01461ee09d0cdfe",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "git_sha": "467c202a876d26af544fa8c4b22a050a535462a7",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "bedops/convert2bed": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
-                    },
-                    "arriba/download": {
-                        "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
-                    },
-                    "gunzip": {
-                        "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "rrnatranscripts": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/quant": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "30a97c755895b7dfe40a730b0695c554a10f1cdd",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -162,20 +208,26 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 51153253a..f6ff09878 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -2,7 +2,6 @@ process FUSIONCATCHER_BUILD {
     tag "fusioncatcher_build"
     label 'process_medium'
 
-    conda "bioconda::fusioncatcher=1.33"
     container "docker.io/clinicalgenomics/fusioncatcher:1.33"
 
     input:
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 916b4673a..20e3681fa 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -7,10 +7,10 @@ process FUSIONCATCHER_DOWNLOAD {
 
 
     input:
-    val ensembl_version
+    val genome_gencode_version
 
     output:
-    path "human_v${ensembl_version}"                , emit: reference
+    path "human_v${genome_gencode_version}"                , emit: reference
     path "versions.yml"     , emit: versions
 
     when:
@@ -24,12 +24,12 @@ process FUSIONCATCHER_DOWNLOAD {
 
     // def url =
     """
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.aa
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ab
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ac
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${ensembl_version}.tar.gz.ad
-    cat human_${ensembl_version}.tar.gz.* | tar xz
-    rm human_${ensembl_version}.tar*
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.aa
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.ab
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.ac
+    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.ad
+    cat human_${genome_gencode_version}.tar.gz.* | tar xz
+    rm human_${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -39,8 +39,8 @@ process FUSIONCATCHER_DOWNLOAD {
 
     stub:
     """
-    mkdir human_v${ensembl_version}
-    touch human_v${ensembl_version}/ensembl_fully_overlapping_genes.txt
+    mkdir human_v${genome_gencode_version}
+    touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
diff --git a/modules/local/gencode_download/main.nf b/modules/local/gencode_download/main.nf
index e9416cf4d..1f466d264 100644
--- a/modules/local/gencode_download/main.nf
+++ b/modules/local/gencode_download/main.nf
@@ -31,7 +31,7 @@ process GENCODE_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
     END_VERSIONS
     """
 
@@ -42,7 +42,7 @@ process GENCODE_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml)
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
     END_VERSIONS
     """
 
diff --git a/modules/local/get_rrna_transcripts.nf b/modules/local/get_rrna_transcripts.nf
new file mode 100644
index 000000000..cb39b3e4c
--- /dev/null
+++ b/modules/local/get_rrna_transcripts.nf
@@ -0,0 +1,43 @@
+process GET_RRNA_TRANSCRIPTS {
+    tag 'get_rrna_bed'
+    label 'process_low'
+
+    conda "bioconda::pirate=1.0.4 bioconda::perl-bioperl=1.7.2"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/pirate:1.0.4--hdfd78af_2' :
+        'biocontainers/pirate:1.0.4--hdfd78af_2' }"
+
+    input:
+    tuple val(meta), path(gtf)
+
+    output:
+    path('rrna.gtf')    , emit: rrnagtf
+    path('rrna.bed')    , emit: bed
+    path "versions.yml" , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    """
+    $baseDir/bin/get_rrna_transcripts.py --gtf ${gtf} --output rrna.gtf
+
+    $baseDir/bin/gtf2bed rrna.gtf > rrna.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        get_rrna_transcripts: v1.0
+    END_VERSIONS
+    """
+
+    stub:
+    """
+    touch rrna.gtf
+    touch rrna.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        get_rrna_transcripts: v1.0
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/arriba/arriba/environment.yml b/modules/nf-core/arriba/arriba/environment.yml
index d0883a0d2..c3a88ffbd 100644
--- a/modules/nf-core/arriba/arriba/environment.yml
+++ b/modules/nf-core/arriba/arriba/environment.yml
@@ -1,5 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
+
 dependencies:
   - bioconda::arriba=2.4.0
diff --git a/modules/nf-core/arriba/arriba/main.nf b/modules/nf-core/arriba/arriba/main.nf
index df6d4e395..75dbf93ad 100644
--- a/modules/nf-core/arriba/arriba/main.nf
+++ b/modules/nf-core/arriba/arriba/main.nf
@@ -8,14 +8,13 @@ process ARRIBA_ARRIBA {
         'biocontainers/arriba:2.4.0--h0033a41_2' }"
 
     input:
-    tuple val(meta), path(bam)
+    tuple val(meta),  path(bam)
     tuple val(meta2), path(fasta)
     tuple val(meta3), path(gtf)
-    tuple val(meta4), path(blacklist)
-    tuple val(meta5), path(known_fusions)
-    tuple val(meta6), path(structural_variants)
-    tuple val(meta7), path(tags)
-    tuple val(meta8), path(protein_domains)
+    path(blacklist)
+    path(known_fusions)
+    path(cytobands)
+    path(protein_domains)
 
     output:
     tuple val(meta), path("*.fusions.tsv")          , emit: fusions
@@ -28,11 +27,10 @@ process ARRIBA_ARRIBA {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def arg_blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
-    def arg_known_fusions = known_fusions ? "-k $known_fusions" : ""
-    def arg_structural_variants = structural_variants ? "-d $structural_variants" : ""
-    def arg_tags = tags ? "-t $tags" : ""
-    def arg_protein_domains = protein_domains ? "-p $protein_domains" : ""
+    def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
+    def known_fusions = known_fusions ? "-k $known_fusions" : ""
+    def cytobands = cytobands ? "-d $cytobands" : ""
+    def protein_domains = protein_domains ? "-p $protein_domains" : ""
 
     """
     arriba \\
@@ -41,11 +39,10 @@ process ARRIBA_ARRIBA {
         -g $gtf \\
         -o ${prefix}.fusions.tsv \\
         -O ${prefix}.fusions.discarded.tsv \\
-        $arg_blacklist \\
-        $arg_known_fusions \\
-        $arg_structural_variants \\
-        $arg_tags \\
-        $arg_protein_domains \\
+        $blacklist \\
+        $known_fusions \\
+        $cytobands \\
+        $protein_domains \\
         $args
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/arriba/arriba/meta.yml b/modules/nf-core/arriba/arriba/meta.yml
index f230dda1f..91ba21039 100644
--- a/modules/nf-core/arriba/arriba/meta.yml
+++ b/modules/nf-core/arriba/arriba/meta.yml
@@ -43,48 +43,19 @@ input:
         type: file
         description: Annotation GTF file
         pattern: "*.{gtf}"
-  - - meta4:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - blacklist:
+  - - blacklist:
         type: file
         description: Blacklist file
         pattern: "*.{tsv}"
-  - - meta5:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - known_fusions:
+  - - known_fusions:
         type: file
         description: Known fusions file
         pattern: "*.{tsv}"
-  - - meta6:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - structural_variants:
-        type: file
-        description: Structural variants file
-        pattern: "*.{tsv}"
-  - - meta7:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - tags:
+  - - cytobands:
         type: file
-        description: Tags file
+        description: Cytobands file
         pattern: "*.{tsv}"
-  - - meta8:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - protein_domains:
+  - - protein_domains:
         type: file
         description: Protein domains file
         pattern: "*.{gff3}"
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test b/modules/nf-core/arriba/arriba/tests/main.nf.test
new file mode 100644
index 000000000..4cff86e50
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test
@@ -0,0 +1,107 @@
+
+nextflow_process {
+
+    name "Test Process ARRIBA_ARRIBA"
+    script "../main.nf"
+    process "ARRIBA_ARRIBA"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "arriba"
+    tag "arriba/arriba"
+    tag "arriba/download"
+    tag "star/genomegenerate"
+    tag "star/align"
+
+    setup {
+        config "./nextflow.config"
+        options "-stub"
+        run("ARRIBA_DOWNLOAD") {
+            script "../../../arriba/download/main.nf"
+            process {
+                """
+                input[0] = 'GRCh38'
+                """
+            }
+        }
+        run("STAR_GENOMEGENERATE") {
+            script "../../../star/genomegenerate/main.nf"
+            options "-stub"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+        run("STAR_ALIGN") {
+            script "../../../star/align/main.nf"
+            options "-stub"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = false
+                input[4] = 'illumina'
+                input[5] = false
+                """
+            }
+        }
+    }
+
+    test("homo_sapiens - paired_end - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.bam', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = []
+
+                input[4] = []
+
+                input[5] = []
+
+                input[6] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test.snap b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
new file mode 100644
index 000000000..14d46f6b8
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
@@ -0,0 +1,55 @@
+{
+    "homo_sapiens - paired_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d323796555db4a58fe4c6bc08d1dec30"
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "fusions_fail": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d323796555db4a58fe4c6bc08d1dec30"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-08T15:41:23.945072"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/arriba/arriba/tests/nextflow.config b/modules/nf-core/arriba/arriba/tests/nextflow.config
new file mode 100644
index 000000000..d3d5b00f6
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/tests/nextflow.config
@@ -0,0 +1,8 @@
+process {
+    withName: STAR_GENOMEGENERATE {
+        ext.args = '--genomeSAindexNbases 11'
+    }
+    withName: STAR_ALIGN {
+        ext.args = '--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50'
+    }
+}
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index 7f11898bd..c45120adb 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -7,16 +7,22 @@ process ARRIBA_DOWNLOAD {
         'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
         'biocontainers/arriba:2.4.0--h0033a41_2' }"
 
+    input:
+    val(genome)
+
     output:
-    path "*"              , emit: reference
-    path "versions.yml"           , emit: versions
+    path "blacklist*${genome}*.tsv.gz"       , emit: blacklist
+    path "cytobands*${genome}*.tsv"          , emit: cytobands
+    path "protein_domains*${genome}*.gff3"   , emit: protein_domains
+    path "known_fusions*${genome}*.tsv.gz"   , emit: known_fusions
+    path "versions.yml"                      , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     """
-    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz
+    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz --no-check-certificate
     tar -xzvf arriba_v2.4.0.tar.gz
     rm arriba_v2.4.0.tar.gz
     mv arriba_v2.4.0/database/* .
@@ -34,7 +40,6 @@ process ARRIBA_DOWNLOAD {
     touch protein_domains_hg38_GRCh38_v2.4.0.gff3
     touch cytobands_hg38_GRCh38_v2.4.0.tsv
     touch known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
-    touch protein_domains_hg38_GRCh38_v2.4.0.gff3
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
index 531c66c2f..bdf542ebc 100644
--- a/modules/nf-core/arriba/download/meta.yml
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -15,12 +15,34 @@ tools:
       licence: ["MIT"]
       identifier: biotools:Arriba
 
+input:
+  - - genome:
+        type: string
+        description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
 output:
-  - reference:
-      - "*":
-          type: directory
-          description: Folder with arriba references
-          pattern: "*"
+  - blacklist:
+      - blacklist*${genome}*.tsv.gz:
+          type: string
+          description: The blacklist removes recurrent alignment artifacts and transcripts
+            which are present in healthy tissue
+          pattern: ".tsv.gz"
+  - cytobands:
+      - cytobands*${genome}*.tsv:
+          type: file
+          description: Coordinates of the Giemsa staining bands. This information is used
+            to draw ideograms
+          pattern: ".tsv"
+  - protein_domains:
+      - protein_domains*${genome}*.gff3:
+          type: file
+          description: Protein domain annotations
+          patter: "*.gff3"
+  - known_fusions:
+      - known_fusions*${genome}*.tsv.gz:
+          type: file
+          description: Arriba is more sensitive to those fusions to improve the detection
+            rate of expected or highly relevant events, such as recurrent fusions
+          patter: "*.tsv.gz"
   - versions:
       - versions.yml:
           type: file
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test b/modules/nf-core/arriba/download/tests/main.nf.test
index cccc95db2..2c32c7a0f 100644
--- a/modules/nf-core/arriba/download/tests/main.nf.test
+++ b/modules/nf-core/arriba/download/tests/main.nf.test
@@ -11,11 +11,10 @@ nextflow_process {
     tag "arriba/download"
 
     test("test-arriba-download") {
-
         when {
             process {
                 """
-
+                input[0] = 'GRCh38'
                 """
             }
         }
@@ -23,11 +22,27 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(
-                    process.out.reference[0].collect { file(it).name }.toSorted(),
-                    process.out.versions
-                    ).match()
-                }
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("download - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = 'GRCh38'
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success }
             )
         }
     }
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
index eda3f7066..fe9b18b94 100644
--- a/modules/nf-core/arriba/download/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/download/tests/main.nf.test.snap
@@ -1,35 +1,43 @@
 {
-    "test-arriba-download": {
+    "download": {
         "content": [
-            [
-                "CREDITS",
-                "RefSeq_viral_genomes_v2.4.0.fa.gz",
-                "blacklist_hg19_hs37d5_GRCh37_v2.4.0.tsv.gz",
-                "blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "blacklist_mm10_GRCm38_v2.4.0.tsv.gz",
-                "blacklist_mm39_GRCm39_v2.4.0.tsv.gz",
-                "cytobands_hg19_hs37d5_GRCh37_v2.4.0.tsv",
-                "cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "cytobands_mm10_GRCm38_v2.4.0.tsv",
-                "cytobands_mm39_GRCm39_v2.4.0.tsv",
-                "known_fusions_hg19_hs37d5_GRCh37_v2.4.0.tsv.gz",
-                "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "known_fusions_mm10_GRCm38_v2.4.0.tsv.gz",
-                "known_fusions_mm39_GRCm39_v2.4.0.tsv.gz",
-                "protein_domains_hg19_hs37d5_GRCh37_v2.4.0.gff3",
-                "protein_domains_hg38_GRCh38_v2.4.0.gff3",
-                "protein_domains_mm10_GRCm38_v2.4.0.gff3",
-                "protein_domains_mm39_GRCm39_v2.4.0.gff3",
-                "versions.yml"
-            ],
-            [
-                "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
-            ]
+            {
+                "0": [
+                    "blacklist_hg38_GRCh38_v2.4.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
+                ],
+                "1": [
+                    "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
+                ],
+                "2": [
+                    "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
+                ],
+                "3": [
+                    "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
+                ],
+                "4": [
+                    "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+                ],
+                "blacklist": [
+                    "blacklist_hg38_GRCh38_v2.4.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
+                ],
+                "cytobands": [
+                    "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
+                ],
+                "known_fusions": [
+                    "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
+                ],
+                "protein_domains": [
+                    "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
+                ],
+                "versions": [
+                    "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+                ]
+            }
         ],
         "meta": {
-            "nf-test": "0.8.4",
+            "nf-test": "0.9.0",
             "nextflow": "24.04.4"
         },
-        "timestamp": "2024-08-22T17:00:38.086459"
+        "timestamp": "2024-10-08T11:12:17.010496"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/arriba/download/tests/tags.yml b/modules/nf-core/arriba/download/tests/tags.yml
new file mode 100644
index 000000000..f510bbf1a
--- /dev/null
+++ b/modules/nf-core/arriba/download/tests/tags.yml
@@ -0,0 +1,2 @@
+arriba/download:
+  - "modules/nf-core/arriba/download/**"
diff --git a/modules/nf-core/gunzip/environment.yml b/modules/nf-core/gunzip/environment.yml
deleted file mode 100644
index c7794856d..000000000
--- a/modules/nf-core/gunzip/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - conda-forge::grep=3.11
-  - conda-forge::sed=4.8
-  - conda-forge::tar=1.34
diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf
deleted file mode 100644
index 5e67e3b9b..000000000
--- a/modules/nf-core/gunzip/main.nf
+++ /dev/null
@@ -1,55 +0,0 @@
-process GUNZIP {
-    tag "$archive"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ubuntu:22.04' :
-        'nf-core/ubuntu:22.04' }"
-
-    input:
-    tuple val(meta), path(archive)
-
-    output:
-    tuple val(meta), path("$gunzip"), emit: gunzip
-    path "versions.yml"             , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args        = task.ext.args ?: ''
-    def extension   = ( archive.toString() - '.gz' ).tokenize('.')[-1]
-    def name        = archive.toString() - '.gz' - ".$extension"
-    def prefix      = task.ext.prefix ?: name
-    gunzip          = prefix + ".$extension"
-    """
-    # Not calling gunzip itself because it creates files
-    # with the original group ownership rather than the
-    # default one for that user / the work directory
-    gzip \\
-        -cd \\
-        $args \\
-        $archive \\
-        > $gunzip
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    def args        = task.ext.args ?: ''
-    def extension   = ( archive.toString() - '.gz' ).tokenize('.')[-1]
-    def name        = archive.toString() - '.gz' - ".$extension"
-    def prefix      = task.ext.prefix ?: name
-    gunzip          = prefix + ".$extension"
-    """
-    touch $gunzip
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml
deleted file mode 100644
index 9066c0353..000000000
--- a/modules/nf-core/gunzip/meta.yml
+++ /dev/null
@@ -1,47 +0,0 @@
-name: gunzip
-description: Compresses and decompresses files.
-keywords:
-  - gunzip
-  - compression
-  - decompression
-tools:
-  - gunzip:
-      description: |
-        gzip is a file format and a software application used for file compression and decompression.
-      documentation: https://www.gnu.org/software/gzip/manual/gzip.html
-      licence: ["GPL-3.0-or-later"]
-      identifier: ""
-input:
-  - - meta:
-        type: map
-        description: |
-          Optional groovy Map containing meta information
-          e.g. [ id:'test', single_end:false ]
-    - archive:
-        type: file
-        description: File to be compressed/uncompressed
-        pattern: "*.*"
-output:
-  - gunzip:
-      - meta:
-          type: file
-          description: Compressed/uncompressed file
-          pattern: "*.*"
-      - $gunzip:
-          type: file
-          description: Compressed/uncompressed file
-          pattern: "*.*"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@jfy133"
-maintainers:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@jfy133"
-  - "@gallvp"
diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test
deleted file mode 100644
index 776211adf..000000000
--- a/modules/nf-core/gunzip/tests/main.nf.test
+++ /dev/null
@@ -1,121 +0,0 @@
-nextflow_process {
-
-    name "Test Process GUNZIP"
-    script "../main.nf"
-    process "GUNZIP"
-    tag "gunzip"
-    tag "modules_nfcore"
-    tag "modules"
-
-    test("Should run without failures") {
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-            process {
-                """
-                input[0] = Channel.of([
-                        [],
-                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
-                    ]
-                )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-            { assert process.success },
-            { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("Should run without failures - prefix") {
-
-        config './nextflow.config'
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-            process {
-                """
-                input[0] = Channel.of([
-                        [ id: 'test' ],
-                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
-                    ]
-                )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-            { assert process.success },
-            { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("Should run without failures - stub") {
-
-        options '-stub'
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-            process {
-                """
-                input[0] = Channel.of([
-                        [],
-                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
-                    ]
-                )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-            { assert process.success },
-            { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("Should run without failures - prefix - stub") {
-
-        options '-stub'
-        config './nextflow.config'
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-            process {
-                """
-                input[0] = Channel.of([
-                        [ id: 'test' ],
-                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)
-                    ]
-                )
-                """
-            }
-        }
-
-        then {
-            assertAll(
-            { assert process.success },
-            { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/gunzip/tests/main.nf.test.snap b/modules/nf-core/gunzip/tests/main.nf.test.snap
deleted file mode 100644
index 069967e76..000000000
--- a/modules/nf-core/gunzip/tests/main.nf.test.snap
+++ /dev/null
@@ -1,134 +0,0 @@
-{
-    "Should run without failures - prefix - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ],
-                "gunzip": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.xyz.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-06-25T11:35:10.861293"
-    },
-    "Should run without failures - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        [
-                            
-                        ],
-                        "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ],
-                "gunzip": [
-                    [
-                        [
-                            
-                        ],
-                        "test_1.fastq:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-06-25T11:35:05.857145"
-    },
-    "Should run without failures": {
-        "content": [
-            {
-                "0": [
-                    [
-                        [
-                            
-                        ],
-                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ],
-                "gunzip": [
-                    [
-                        [
-                            
-                        ],
-                        "test_1.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2023-10-17T15:35:37.690477896"
-    },
-    "Should run without failures - prefix": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ],
-                "gunzip": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.xyz.fastq:md5,4161df271f9bfcd25d5845a1e220dbec"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,54376d32aca20e937a4ec26dac228e84"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
-        },
-        "timestamp": "2024-06-25T11:33:32.921739"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/gunzip/tests/nextflow.config b/modules/nf-core/gunzip/tests/nextflow.config
deleted file mode 100644
index dec776425..000000000
--- a/modules/nf-core/gunzip/tests/nextflow.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: GUNZIP {
-        ext.prefix = { "${meta.id}.xyz" }
-    }
-}
diff --git a/modules/nf-core/gunzip/tests/tags.yml b/modules/nf-core/gunzip/tests/tags.yml
deleted file mode 100644
index fd3f69154..000000000
--- a/modules/nf-core/gunzip/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-gunzip:
-  - modules/nf-core/gunzip/**
diff --git a/modules/nf-core/salmon/quant/environment.yml b/modules/nf-core/salmon/quant/environment.yml
index 471164faa..b3f75777e 100644
--- a/modules/nf-core/salmon/quant/environment.yml
+++ b/modules/nf-core/salmon/quant/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::salmon=1.10.1
+  - bioconda::salmon=1.10.3
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index 6d624e751..f1e3b5cda 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -4,8 +4,8 @@ process SALMON_QUANT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/salmon:1.10.1--h7e5ed60_0' :
-        'biocontainers/salmon:1.10.1--h7e5ed60_0' }"
+        'https://depot.galaxyproject.org/singularity/salmon:1.10.3--h6dccd9a_2' :
+        'biocontainers/salmon:1.10.3--h6dccd9a_2' }"
 
     input:
     tuple val(meta), path(reads)
@@ -28,7 +28,7 @@ process SALMON_QUANT {
     def args = task.ext.args   ?: ''
     prefix   = task.ext.prefix ?: "${meta.id}"
 
-    def reference = "--index $index"
+    def reference   = "--index $index"
     def reads1 = [], reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def input_reads = meta.single_end ? "-r ${reads1.join(" ")}" : "-1 ${reads1.join(" ")} -2 ${reads2.join(" ")}"
diff --git a/modules/nf-core/salmon/quant/tests/main.nf.test.snap b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
index 547ce2de1..ea22a80ce 100644
--- a/modules/nf-core/salmon/quant/tests/main.nf.test.snap
+++ b/modules/nf-core/salmon/quant/tests/main.nf.test.snap
@@ -2,7 +2,7 @@
     "sarscov2 - single_end": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -15,15 +15,15 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:51:22.424672"
+        "timestamp": "2024-10-18T10:01:16.989080539"
     },
     "sarscov2 - single_end stub": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -36,15 +36,15 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:51:32.292277"
+        "timestamp": "2024-10-18T10:01:29.340996235"
     },
     "sarscov2 - single_end lib type A": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -57,15 +57,15 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:51:42.656382"
+        "timestamp": "2024-10-18T10:01:43.056167576"
     },
     "sarscov2 - pair_end multiple": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -78,15 +78,15 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:52:33.03647"
+        "timestamp": "2024-10-18T10:03:05.500792631"
     },
     "sarscov2 - pair_end multiple stub": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -99,15 +99,15 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:52:43.388379"
+        "timestamp": "2024-10-18T10:03:26.428959203"
     },
     "sarscov2 - single_end lib type A stub": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -120,15 +120,15 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:51:52.612664"
+        "timestamp": "2024-10-18T10:02:03.420850208"
     },
     "sarscov2 - pair_end": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -141,15 +141,15 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:52:05.577881"
+        "timestamp": "2024-10-18T10:02:16.130074696"
     },
     "sarscov2 - pair_end stub": {
         "content": [
             [
-                "versions.yml:md5,80eb3d2ad36960c7e9263f81ede9d263"
+                "versions.yml:md5,0d510d5db6398e2c8ca9443330740607"
             ],
             [
                 [
@@ -162,9 +162,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-06-14T09:52:15.286461"
+        "timestamp": "2024-10-18T10:02:39.470004547"
     }
 }
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 3e4e4b4d1..98535a017 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -11,7 +11,7 @@ params {
 
     // Input options
     input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-    build_references           = false
+    references_only            = false
     cosmic_username            = null
     cosmic_passwd              = null
     qiagen                     = false
@@ -75,6 +75,7 @@ params {
     rrna_intervals                = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.interval_list"
     gencode_ref                   = "${params.genomes_base}/gencode"
     arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz"
+    arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv"
     arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz"
     arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 480aa0917..159155108 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -79,8 +79,7 @@
                 "genome_gencode_version": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
-                    "description": "ensembl version",
-                    "default": 102
+                    "description": "gencode version"
                 },
                 "starfusion_build": {
                     "type": "boolean",
@@ -108,6 +107,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
                 },
+                "arriba_ref_cytobands": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Path to arriba reference cytobands"
+                },
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
@@ -307,9 +311,9 @@
             "fa_icon": "fas fa-dna",
             "description": "Reference genome related files and options required for the workflow.",
             "properties": {
-                "genome": {
-                    "type": "string",
-                    "description": "Name of iGenomes reference.",
+                "references_only": {
+                    "type": "boolean",
+                    "description": "Skip running the analysis, only builds the references",
                     "fa_icon": "fas fa-book"
                 },
                 "fasta": {
@@ -328,6 +332,11 @@
                     "description": "Path to FASTA genome index file.",
                     "fa_icon": "far fa-file-code"
                 },
+                "genome": {
+                    "type": "string",
+                    "description": "Name of iGenomes reference.",
+                    "fa_icon": "fas fa-book"
+                },
                 "gtf": {
                     "type": "string",
                     "format": "file-path",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 1d45e74ca..0c6c99164 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -4,7 +4,6 @@
 ========================================================================================
 */
 
-include { ARRIBA_DOWNLOAD }                 from '../../modules/local/arriba/download/main'
 include { GENCODE_DOWNLOAD }                from '../../modules/local/gencode_download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../../modules/local/fusioncatcher/download/main'
 include { FUSIONCATCHER_BUILD }             from '../../modules/local/fusioncatcher/build/main'
@@ -14,13 +13,14 @@ include { STARFUSION_BUILD }                from '../../modules/local/starfusion
 include { STARFUSION_DOWNLOAD }             from '../../modules/local/starfusion/download/main'
 include { GTF_TO_REFFLAT }                  from '../../modules/local/uscs/custom_gtftogenepred/main'
 include { RRNA_TRANSCRIPTS }                from '../../modules/local/rrnatranscripts/main'
-include { CONVERT2BED }                     from '../../modules/local/convert2bed/main'
+include { GET_RRNA_TRANSCRIPTS }            from '../../modules/local/get_rrna_transcripts'
+// include { CONVERT2BED }                     from '../../modules/local/convert2bed/main'
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
-
+include { ARRIBA_DOWNLOAD }                 from '../../modules/nf-core/arriba/download/main'
 include { SAMTOOLS_FAIDX }                  from '../../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../../modules/nf-core/star/genomegenerate/main'
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../modules/nf-core/gatk4/createsequencedictionary/main'
@@ -73,9 +73,8 @@ workflow BUILD_REFERENCES {
 
     if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty()){
         GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
-        RRNA_TRANSCRIPTS(ch_gtf)
-        CONVERT2BED(RRNA_TRANSCRIPTS.out.rrna_gtf)
-        GATK4_BEDTOINTERVALLIST(CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
+        GET_RRNA_TRANSCRIPTS(ch_gtf)
+        GATK4_BEDTOINTERVALLIST(GET_RRNA_TRANSCRIPTS.out.bed.map { it -> [ [id:it.name], it ] }, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
         ch_rrna_interval = GATK4_BEDTOINTERVALLIST.out.interval_list
     } else {
         ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
@@ -106,31 +105,35 @@ workflow BUILD_REFERENCES {
         ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
     }
 
-//     if ((params.arriba || params.all) &&
-//             (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
-//             !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
-//             !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
-//         ARRIBA_DOWNLOAD()
-//         ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
-//         ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
-//         ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
-//     } else {
-// // TODO need to update the module to emit blacklist,knownfusions etc
-//     }
-
-
-    // if ((params.fusioncatcher || params.all) &&
-    //         (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
-    //         !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
-    //     if (params.download_refs) {
-    //         FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
-    //         ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference}
-    //     else {
-    //         FUSIONCATCHER_BUILD(params.genome_gencode_version)
-    //         ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference}
-    //     } else {
-    //         ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
-    //     }
+    if ((params.arriba || params.all) &&
+            (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
+            !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
+            !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
+        ARRIBA_DOWNLOAD(genome)
+        ch_arriba_ref_blacklist = ARRIBA_DOWNLOAD.out.blacklist
+        ch_arriba_ref_cytobands = ARRIBA_DOWNLOAD.out.cytobands
+        ch_arriba_ref_known_fusions = ARRIBA_DOWNLOAD.out.known_fusions
+        ch_arriba_ref_protein_domains = ARRIBA_DOWNLOAD.out.protein_domains
+    } else {
+        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
+        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { that -> [[id:that.Name], that] }
+        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
+        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
+    }
+
+
+    if ((params.fusioncatcher || params.all) &&
+            (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
+            !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
+        if (params.download_refs) {
+            FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
+            ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference}
+        else {
+            FUSIONCATCHER_BUILD(params.genome_gencode_version)
+            ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference}
+        } else {
+            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
+        }
 
 
     if ((params.starfusion || params.all) &&
@@ -144,14 +147,14 @@ workflow BUILD_REFERENCES {
         ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }}
 
 
-    // if ((params.fusionreport || params.all) &&
-    //         (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
-    //         !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
-    //     if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
-    //     ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd ).out.reference
-    // } else {
-    //     ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
-    // }
+    if ((params.fusionreport || params.all) &&
+            (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
+            !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
+        if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
+        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd ).out.reference
+    } else {
+        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
+    }
 
     emit:
     ch_fasta
@@ -160,16 +163,17 @@ workflow BUILD_REFERENCES {
 
     ch_hgnc_ref
     ch_hgnc_date
-    // ch_rrna_interval
-    // ch_refflat
-    // ch_salmon_index
-    // ch_starindex_ref
-    // ch_arriba_ref_blacklist
-    // ch_arriba_ref_known_fusions
-    // ch_arriba_ref_protein_domains
-    // ch_fusioncatcher_ref
-    // ch_starfusion_ref
-    // ch_fusionreport_ref
+    ch_rrna_interval
+    ch_refflat
+    ch_salmon_index
+    ch_starindex_ref
+    ch_arriba_ref_blacklist
+    ch_arriba_ref_cytobands
+    ch_arriba_ref_known_fusions
+    ch_arriba_ref_protein_domains
+    ch_fusioncatcher_ref
+    ch_starfusion_ref
+    ch_fusionreport_ref
     versions        = ch_versions
 }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 8f7f8ae8a..5e34db974 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -4,41 +4,21 @@
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 */
 
-
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT LOCAL MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules
-//
 include { BUILD_REFERENCES              }   from '../subworkflows/local/build_references'
-include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
-include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
-include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
-include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
-include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow'
-include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
-include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
-include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
-include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    IMPORT NF-CORE MODULES/SUBWORKFLOWS
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// MODULE: Installed directly from nf-core/modules
-//
-include { CAT_FASTQ              } from '../modules/nf-core/cat/fastq/main'
-include { FASTQC                 } from '../modules/nf-core/fastqc/main'
-include { MULTIQC                } from '../modules/nf-core/multiqc/main'
-include { SALMON_QUANT           } from '../modules/nf-core/salmon/quant/main'
+// include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
+// include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
+// include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
+// include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
+// include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow'
+// include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
+// include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
+// include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
+// include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
+
+include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
+include { FASTQC                        }   from '../modules/nf-core/fastqc/main'
+include { MULTIQC                       }   from '../modules/nf-core/multiqc/main'
+// include { SALMON_QUANT                  }   from '../modules/nf-core/salmon/quant/main'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -54,7 +34,7 @@ workflow RNAFUSION {
 
 
     ch_versions = Channel.empty()
-    ch_multiqc_files = Channel.empty()
+    // ch_multiqc_files = Channel.empty()
 
     //
     // Create references if necessary
@@ -268,7 +248,7 @@ workflow RNAFUSION {
     // QC_WORKFLOW (
     //     ch_reads_all,
     //     STARFUSION_WORKFLOW.out.ch_bam_sorted,
-    //     ch_chrgtf,
+    //     ch_gtf,
     //     ch_refflat,
     //     ch_fasta,
     //     ch_fai,

From d18b303eb7a1644405fe877bc7a2a80f88197f19 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Nov 2024 15:45:10 +0100
Subject: [PATCH 079/887] update modules, remove chrgtf, adapt with meta/no
 meta

---
 .github/workflows/awsfulltest.yml             |  18 +-
 .github/workflows/awstest.yml                 |  26 --
 .github/workflows/ci.yml                      |   9 +-
 AAA_BBB.html                                  |   0
 README.md                                     |   6 +-
 conf/modules.config                           |   6 +-
 docs/usage.md                                 |  16 +-
 modules.json                                  | 146 +++------
 modules/local/fusionreport/detect/main.nf     |  12 +-
 modules/nf-core/cat/fastq/environment.yml     |   2 +-
 modules/nf-core/cat/fastq/main.nf             |   4 +-
 .../nf-core/cat/fastq/tests/main.nf.test.snap | 100 +++---
 .../collectinsertsizemetrics/environment.yml  |   2 +-
 .../picard/collectinsertsizemetrics/main.nf   |   4 +-
 .../tests/main.nf.test.snap                   |  14 +-
 .../collectrnaseqmetrics/environment.yml      |   2 +-
 .../picard/collectrnaseqmetrics/main.nf       |   4 +-
 .../collectrnaseqmetrics/tests/main.nf.test   |  81 +++++
 .../tests/main.nf.test.snap                   |  60 ++++
 .../tests/nextflow.config                     |  13 +
 .../picard/collectwgsmetrics/environment.yml  |   4 +-
 .../nf-core/picard/collectwgsmetrics/main.nf  |   4 +-
 .../collectwgsmetrics/tests/main.nf.test.snap |  12 +-
 modules/nf-core/salmon/index/environment.yml  |   2 +-
 modules/nf-core/salmon/index/main.nf          |   4 +-
 .../salmon/index/tests/main.nf.test.snap      |  16 +-
 modules/nf-core/salmon/quant/main.nf          |   3 +-
 modules/nf-core/samtools/sort/main.nf         |  10 +-
 .../nf-core/samtools/sort/tests/main.nf.test  |  64 ++++
 .../samtools/sort/tests/main.nf.test.snap     |  99 +++++-
 modules/nf-core/samtools/view/environment.yml |   2 +
 modules/nf-core/samtools/view/main.nf         |   4 +-
 modules/nf-core/star/align/environment.yml    |   6 +-
 modules/nf-core/star/align/main.nf            |   4 +-
 modules/nf-core/star/align/meta.yml           | 111 ++++---
 modules/nf-core/star/align/tests/main.nf.test |  36 +-
 .../star/align/tests/main.nf.test.snap        | 190 +++--------
 .../star/genomegenerate/environment.yml       |   6 +-
 modules/nf-core/star/genomegenerate/main.nf   |   4 +-
 .../genomegenerate/tests/main.nf.test.snap    |  28 +-
 .../stringtie/stringtie/environment.yml       |   2 +-
 modules/nf-core/stringtie/stringtie/main.nf   |   4 +-
 .../stringtie/tests/main.nf.test.snap         |  64 ++--
 subworkflows/local/arriba_workflow.nf         |   3 +-
 subworkflows/local/build_references.nf        |  46 ++-
 subworkflows/local/fusionreport_workflow.nf   |   2 +-
 test.fusionreport.tsv                         |   0
 test.fusionreport_filtered.tsv                |   0
 test.fusions.csv                              |   0
 test.fusions.json                             |   0
 test_fusionreport_index.html                  |   0
 tests/main.nf.test                            |  22 +-
 workflows/rnafusion.nf                        | 307 ++++++------------
 53 files changed, 785 insertions(+), 799 deletions(-)
 create mode 100644 AAA_BBB.html
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config
 create mode 100644 test.fusionreport.tsv
 create mode 100644 test.fusionreport_filtered.tsv
 create mode 100644 test.fusions.csv
 create mode 100644 test.fusions.json
 create mode 100644 test_fusionreport_index.html

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index fe30ca560..d1492d8b8 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -46,7 +46,6 @@ jobs:
               "cosmic_username": "${{ secrets.cosmic_username }}",
               "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
               "all": true,
-              "build_references": true
             }
           profiles: test_full,aws_tower
       - uses: actions/upload-artifact@v4
@@ -55,19 +54,4 @@ jobs:
           path: |
             seqera_platform_action_*.log
             seqera_platform_action_*.json
-      - name: Launch run workflow via tower
-        uses: seqeralabs/action-tower-launch@v2
-        with:
-          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
-          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
-          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
-          parameters: |
-            {
-              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}",
-              "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}/references",
-              "cosmic_username": "${{ secrets.cosmic_username }}",
-              "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
-              "all": true,
-            }
-          profiles: test_full,aws_tower
+
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 8f6bfb014..78bdb317c 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -26,8 +26,6 @@ jobs:
               "cosmic_username": "${{ secrets.cosmic_username }}",
               "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
               "all": true,
-              "stub": true,
-              "build_references": true
             }
           profiles: test,aws_tower
       - uses: actions/upload-artifact@v4
@@ -36,27 +34,3 @@ jobs:
           path: |
             tower_action_*.log
             tower_action_*.json
-
-      - name: Launch workflow via tower
-        uses: seqeralabs/action-tower-launch@v2
-        with:
-          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
-          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
-          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
-          parameters: |
-            {
-              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}",
-              "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}/references",
-              "cosmic_username": "${{ secrets.cosmic_username }}",
-              "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
-              "all": true,
-              "stub": true
-            }
-          profiles: test,aws_tower
-      - uses: actions/upload-artifact@v4
-        with:
-          name: Seqera Platform debug log file
-          path: |
-            seqera_platform_action_*.log
-            seqera_platform_action_*.json
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index e1028ed8e..2a87529d6 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -83,16 +83,11 @@ jobs:
       - name: Clean up Disk space
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - name: "Run pipeline build with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
+      - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} \
-          -stub --build_references \
+          -stub \
           --outdir /home/runner/work/rnafusion/rnafusion/results --all \
           --genomes_base /home/runner/work/rnafusion/rnafusion/results/references \
           --cosmic_username ${{ secrets.COSMIC_USERNAME }} --cosmic_passwd ${{ secrets.COSMIC_PASSWD }}
 
-      - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
-        run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} -stub \
-          --outdir /home/runner/work/rnafusion/rnafusion/results --all ${{ matrix.trim_parameters }} \
-          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
diff --git a/AAA_BBB.html b/AAA_BBB.html
new file mode 100644
index 000000000..e69de29bb
diff --git a/README.md b/README.md
index 41e2bc96a..a52100916 100644
--- a/README.md
+++ b/README.md
@@ -31,9 +31,9 @@ In rnafusion the full-sized test includes reference building and fusion detectio
 
 ### Build references
 
-`--build_references` triggers a parallel workflow to build references, which is a prerequisite to running the pipeline:
+`--references_only` triggers a workflow to ONLY build references, otherwise the references are built with the analysis run:
 
-1. Download ensembl fasta and gtf files
+1. Download gencode fasta and gtf files
 2. Create [STAR](https://github.com/alexdobin/STAR) index
 3. Download [Arriba](https://github.com/suhrig/arriba) references
 4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references
@@ -78,7 +78,7 @@ First, build the references:
 nextflow run nf-core/rnafusion \
    -profile test,<docker/singularity/.../institute> \
    --outdir <OUTDIR>\
-   --build_references \
+   --references_only \
    -stub
 ```
 
diff --git a/conf/modules.config b/conf/modules.config
index 123e02b08..b07f53335 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -46,7 +46,7 @@ process {
     }
 
     withName: 'CAT_FASTQ' {
-        ext.when   = { !params.build_references }
+        ext.when   = { !params.references_only }
     }
 
     withName: 'GENCODE_DOWNLOAD' {
@@ -63,7 +63,7 @@ process {
 
     withName: 'FASTQC' {
         ext.args = '--quiet'
-        ext.when = { !params.skip_qc && !params.build_references }
+        ext.when = { !params.skip_qc && !params.references_only }
         publishDir = [
             path: { "${params.outdir}/fastqc" },
             mode: params.publish_dir_mode,
@@ -367,7 +367,7 @@ process {
     }
 
     withName: 'TRIM_WORKFLOW:*' {
-        ext.when = { !params.build_references }
+        ext.when = { !params.references_only }
     }
 
     withName: 'VCF_COLLECT' {
diff --git a/docs/usage.md b/docs/usage.md
index b7bc6794c..86ede4ddc 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -10,7 +10,7 @@ The pipeline is divided into two parts:
 
 1. Download and build references
 
-- specified with `--build_references` parameter
+- specified with `--references_only` parameter
 - required only once before running the pipeline
 - **Important**: has to be run with each new release
 
@@ -32,7 +32,7 @@ The rnafusion pipeline needs references for the fusion detection tools, so downl
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --build_references --all \
+  --references_only --all \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <PATH/TO/REFERENCES>
@@ -43,7 +43,7 @@ References for each tools can also be downloaded separately with:
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --build_references --<tool1> --<tool2> ... \
+  --references_only --<tool1> --<tool2> ... \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH>
@@ -62,7 +62,7 @@ Use credentials from QIAGEN and add `--qiagen`
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --build_references --<tool1> --<tool2> ... \
+  --references_only --<tool1> --<tool2> ... \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH> --qiagen
@@ -79,7 +79,7 @@ If process `FUSIONREPORT_DOWNLOAD` times out, it could be due to network restric
 ```bash
 nextflow run nf-core/rnafusion  \
   -profile <docker/singularity/.../institute> \
-  --build_references \
+  --references_only \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --fusionreport \
   --genomes_base <PATH/TO/REFERENCES> \
@@ -91,7 +91,7 @@ Where the custom configuration could look like (adaptation to local machine nece
 
 ```text
 process {
-  withName:  'NFCORE_RNAFUSION:BUILD_REFERENCES:FUSIONREPORT_DOWNLOAD' {
+  withName:  'NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONREPORT_DOWNLOAD' {
     memory = '8.GB'
     cpus = 4
   }
@@ -158,7 +158,7 @@ nextflow run nf-core/rnafusion \
 
 > **IMPORTANT: Either `--all` or `--<tool>`** is necessary to run detection tools
 
-`--genomes_base` should be the path to the directory containing the folder `references/` that was built with `--build_references`.
+`--genomes_base` should be the path to the directory containing the folder `references/` that was built with `--references_only`.
 
 Note that the pipeline will create the following files in your working directory:
 
@@ -393,7 +393,7 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
 - `test`
   - A profile with a complete configuration for automated testing
   - Includes links to test data so needs no other parameters
-  - Needs to run in two steps: with `--build_references` first and then without `--build_references` to run the analysis
+  - Needs to run in two steps: with `--references_only` first and then without `--references_only` to run the analysis
   - !!!! Run with `-stub` as all references need to be downloaded otherwise !!!!
 
 ### `-resume`
diff --git a/modules.json b/modules.json
index 7e5065489..358910d4a 100644
--- a/modules.json
+++ b/modules.json
@@ -8,198 +8,142 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "7741dfc830e77a8ead2fcb50b01461ee09d0cdfe",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/download": {
                         "branch": "master",
                         "git_sha": "467c202a876d26af544fa8c4b22a050a535462a7",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bedops/convert2bed": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "a1abf90966a2a4016d3c3e41e228bfcbd4811ccc",
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
+                        "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
+                        "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
+                        "installed_by": ["modules"]
                     },
                     "rrnatranscripts": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
+                        "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "b7800db9b069ed505db3f9d91b8c72faea9be17b",
+                        "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
-                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "669eb24fd82a9d3cb18ad0e73673ecb26827f683",
+                        "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "30a97c755895b7dfe40a730b0695c554a10f1cdd",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "a5ad53288c79fa52c5ae708c317e09ec2dd149ab",
+                        "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "a5ad53288c79fa52c5ae708c317e09ec2dd149ab",
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
+                        "installed_by": ["modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -208,26 +152,20 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 8024d8f8e..f2557bf01 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -8,17 +8,17 @@ process FUSIONREPORT {
 
     input:
     tuple val(meta), path(reads), path(arriba_fusions), path(starfusion_fusions),  path(fusioncatcher_fusions)
-    tuple val(meta2), path(fusionreport_ref)
+    path(fusionreport_ref)
     val(tools_cutoff)
 
     output:
-    path "versions.yml"                                                 , emit: versions
     tuple val(meta), path("*fusionreport.tsv")                          , emit: fusion_list
     tuple val(meta), path("*fusionreport_filtered.tsv")                 , emit: fusion_list_filtered
-    tuple val(meta), path("*index.html")                                 , emit: report
+    tuple val(meta), path("*index.html")                                , emit: report
     tuple val(meta), path("*_*.html")                    , optional:true, emit: html
     tuple val(meta), path("*.csv")                       , optional:true, emit: csv
     tuple val(meta), path("*.json")                      , optional:true, emit: json
+    path "versions.yml"                                                 , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -58,7 +58,11 @@ process FUSIONREPORT {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusion_report: \$(fusion_report --version | sed 's/fusion-report //')
+        fusion_report: sth
     END_VERSIONS
     """
 }
+    // cat <<-END_VERSIONS > versions.yml
+    // "${task.process}":
+    //     fusion_report: \$(fusion_report --version 2>&1 | sed 's/fusion-report //')
+    // END_VERSIONS
diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml
index c7eb9bd1c..71e04c3d7 100644
--- a/modules/nf-core/cat/fastq/environment.yml
+++ b/modules/nf-core/cat/fastq/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::coreutils=8.30
+  - conda-forge::coreutils=9.5
diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf
index b68e5f911..4364a389b 100644
--- a/modules/nf-core/cat/fastq/main.nf
+++ b/modules/nf-core/cat/fastq/main.nf
@@ -4,8 +4,8 @@ process CAT_FASTQ {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
-        'nf-core/ubuntu:20.04' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c2/c262fc09eca59edb5a724080eeceb00fb06396f510aefb229c2d2c6897e63975/data' :
+        'community.wave.seqera.io/library/coreutils:9.5--ae99c88a9b28c264' }"
 
     input:
     tuple val(meta), path(reads, stageAs: "input*/*")
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test.snap b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
index aec119a94..f8689a1ce 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test.snap
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
@@ -12,7 +12,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -24,15 +24,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-01-17T17:30:39.816981"
+        "timestamp": "2024-10-19T20:02:07.519211144"
     },
     "test_cat_fastq_single_end_same_name": {
         "content": [
@@ -47,7 +47,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -59,15 +59,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-01-17T17:32:35.229332"
+        "timestamp": "2024-10-19T20:02:31.618628921"
     },
     "test_cat_fastq_single_end_single_file": {
         "content": [
@@ -82,7 +82,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -94,15 +94,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-01-17T17:34:00.058829"
+        "timestamp": "2024-10-19T20:02:57.904149581"
     },
     "test_cat_fastq_paired_end_same_name": {
         "content": [
@@ -120,7 +120,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -135,15 +135,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-01-17T17:33:33.031555"
+        "timestamp": "2024-10-19T20:02:44.577183829"
     },
     "test_cat_fastq_single_end - stub": {
         "content": [
@@ -158,7 +158,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -170,15 +170,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-05T12:07:28.244999"
+        "timestamp": "2024-10-19T20:03:10.603734777"
     },
     "test_cat_fastq_paired_end_same_name - stub": {
         "content": [
@@ -196,7 +196,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -211,15 +211,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-05T12:07:57.070911"
+        "timestamp": "2024-10-19T20:03:46.041808828"
     },
     "test_cat_fastq_single_end_same_name - stub": {
         "content": [
@@ -234,7 +234,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -246,15 +246,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-05T12:07:46.796254"
+        "timestamp": "2024-10-19T20:03:34.13865402"
     },
     "test_cat_fastq_paired_end": {
         "content": [
@@ -272,7 +272,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -287,15 +287,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-01-17T17:32:02.270935"
+        "timestamp": "2024-10-19T20:02:19.64383573"
     },
     "test_cat_fastq_paired_end - stub": {
         "content": [
@@ -313,7 +313,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -328,15 +328,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-05T12:07:37.807553"
+        "timestamp": "2024-10-19T20:03:22.597246066"
     },
     "test_cat_fastq_single_end_single_file - stub": {
         "content": [
@@ -351,7 +351,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ],
                 "reads": [
                     [
@@ -363,14 +363,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d42d6e24d67004608495883e00bd501b"
+                    "versions.yml:md5,6ef4fd28546a005865b9454bbedbf81a"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-05T12:14:51.861264"
+        "timestamp": "2024-10-19T20:03:58.44849001"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
index ff4a85edb..1d715d564 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
+++ b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::picard=3.2.0
+  - bioconda::picard=3.3.0
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/main.nf b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
index 1a49a7f0a..c3014d806 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/main.nf
+++ b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTINSERTSIZEMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.2.0--hdfd78af_0' :
-        'biocontainers/picard:3.2.0--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.3.0--hdfd78af_0' :
+        'biocontainers/picard:3.3.0--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap b/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap
index bcb5b4557..cbe9329d0 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap
+++ b/modules/nf-core/picard/collectinsertsizemetrics/tests/main.nf.test.snap
@@ -9,14 +9,14 @@
             ],
             "test.pdf",
             [
-                "versions.yml:md5,4423bfe2194f61d919c382d2225b0835"
+                "versions.yml:md5,38d39e9882afe7ac015213c286745056"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
+            "nf-test": "0.9.0",
             "nextflow": "24.04.4"
         },
-        "timestamp": "2024-08-30T12:23:29.392944"
+        "timestamp": "2024-10-18T10:55:45.769771444"
     },
     "test-picard-collectinsertsizemetrics-stub": {
         "content": [
@@ -40,7 +40,7 @@
                     ]
                 ],
                 "2": [
-                    "versions.yml:md5,4423bfe2194f61d919c382d2225b0835"
+                    "versions.yml:md5,38d39e9882afe7ac015213c286745056"
                 ],
                 "histogram": [
                     [
@@ -61,14 +61,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,4423bfe2194f61d919c382d2225b0835"
+                    "versions.yml:md5,38d39e9882afe7ac015213c286745056"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
+            "nf-test": "0.9.0",
             "nextflow": "24.04.4"
         },
-        "timestamp": "2024-08-30T12:20:03.841884"
+        "timestamp": "2024-10-18T10:56:09.914953495"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
index ff4a85edb..1d715d564 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
+++ b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::picard=3.2.0
+  - bioconda::picard=3.3.0
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/main.nf b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
index 0c8f2cfa2..eb80fdc70 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/main.nf
+++ b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTRNASEQMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.2.0--hdfd78af_0' :
-        'biocontainers/picard:3.2.0--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.3.0--hdfd78af_0' :
+        'biocontainers/picard:3.3.0--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test
new file mode 100644
index 000000000..9ab185521
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test
@@ -0,0 +1,81 @@
+nextflow_process {
+
+    name "Test Process PICARD_COLLECTRNASEQMETRICS"
+    script "../main.nf"
+    process "PICARD_COLLECTRNASEQMETRICS"
+    config "./nextflow.config"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "picard"
+    tag "picard/collectrnaseqmetrics"
+    tag "ucsc/gtftogenepred"
+
+    setup {
+        run("UCSC_GTFTOGENEPRED") {
+            script "../../../ucsc/gtftogenepred/main.nf"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+    }
+
+    test("sarscov2 - fasta - gtf") {
+        
+        when {
+            process {
+                """
+                input[0] = [
+        	    [ id:'test', single_end:false, strandedness:'forward' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = UCSC_GTFTOGENEPRED.out.refflat.map{ it[1] }
+                input[2] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert file(process.out.metrics[0][1]).text.contains('CollectRnaSeqMetrics') },
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.pdf
+                ).match() }
+            )
+        }
+    }
+    
+    test("sarscov2 - fasta - gtf - stub") {
+        
+        options "-stub"
+        
+        when {
+            process {
+                """
+                input[0] = [
+        	    [ id:'test', single_end:false, strandedness:'forward' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = UCSC_GTFTOGENEPRED.out.refflat.map{ it[1] }
+                input[2] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap
new file mode 100644
index 000000000..ad6503afd
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap
@@ -0,0 +1,60 @@
+{
+    "sarscov2 - fasta - gtf": {
+        "content": [
+            [
+                "versions.yml:md5,8d0867f89947c081711de09338138c2e"
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T16:10:39.199344417"
+    },
+    "sarscov2 - fasta - gtf - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false,
+                            "strandedness": "forward"
+                        },
+                        "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,8d0867f89947c081711de09338138c2e"
+                ],
+                "metrics": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false,
+                            "strandedness": "forward"
+                        },
+                        "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "pdf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,8d0867f89947c081711de09338138c2e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T16:10:57.248132065"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config b/modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config
new file mode 100644
index 000000000..bc82e10c1
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config
@@ -0,0 +1,13 @@
+process {
+    withName:UCSC_GTFTOGENEPRED {
+        ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons"
+    }
+    withName:PICARD_COLLECTRNASEQMETRICS {
+        ext.args = { ( meta.strandedness == "forward" || meta.single_end ) ?
+            "--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND" :
+        meta.strandedness == "reverse" ?
+        "--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND" :
+        "--STRAND_SPECIFICITY NONE"
+    }
+    }
+}
diff --git a/modules/nf-core/picard/collectwgsmetrics/environment.yml b/modules/nf-core/picard/collectwgsmetrics/environment.yml
index 58d525818..13265842f 100644
--- a/modules/nf-core/picard/collectwgsmetrics/environment.yml
+++ b/modules/nf-core/picard/collectwgsmetrics/environment.yml
@@ -2,5 +2,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::picard=3.2.0
-  - r::r-base
+  - bioconda::picard=3.3.0
+  - conda-forge::r-base=4.4.1
diff --git a/modules/nf-core/picard/collectwgsmetrics/main.nf b/modules/nf-core/picard/collectwgsmetrics/main.nf
index 6002a7ca9..39cf7d2b7 100644
--- a/modules/nf-core/picard/collectwgsmetrics/main.nf
+++ b/modules/nf-core/picard/collectwgsmetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTWGSMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.2.0--hdfd78af_0' :
-        'biocontainers/picard:3.2.0--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.3.0--hdfd78af_0' :
+        'biocontainers/picard:3.3.0--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
diff --git a/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap b/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap
index f188382b0..1958fcdeb 100644
--- a/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap
+++ b/modules/nf-core/picard/collectwgsmetrics/tests/main.nf.test.snap
@@ -3,26 +3,26 @@
         "content": [
             true,
             [
-                "versions.yml:md5,06b5898fb06823b736c90e1dcebe75fe"
+                "versions.yml:md5,9927db69fdd55176be5cdbd427d000c2"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
+            "nf-test": "0.9.0",
             "nextflow": "24.04.4"
         },
-        "timestamp": "2024-08-23T11:02:46.301176"
+        "timestamp": "2024-10-18T10:15:18.13771243"
     },
     "test-picard-collectwgsmetrics": {
         "content": [
             true,
             [
-                "versions.yml:md5,06b5898fb06823b736c90e1dcebe75fe"
+                "versions.yml:md5,9927db69fdd55176be5cdbd427d000c2"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
+            "nf-test": "0.9.0",
             "nextflow": "24.04.4"
         },
-        "timestamp": "2024-08-23T11:02:25.132069"
+        "timestamp": "2024-10-18T10:14:57.786056996"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/salmon/index/environment.yml b/modules/nf-core/salmon/index/environment.yml
index 471164faa..b3f75777e 100644
--- a/modules/nf-core/salmon/index/environment.yml
+++ b/modules/nf-core/salmon/index/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::salmon=1.10.1
+  - bioconda::salmon=1.10.3
diff --git a/modules/nf-core/salmon/index/main.nf b/modules/nf-core/salmon/index/main.nf
index e755d9a34..3d653c0d0 100644
--- a/modules/nf-core/salmon/index/main.nf
+++ b/modules/nf-core/salmon/index/main.nf
@@ -4,8 +4,8 @@ process SALMON_INDEX {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/salmon:1.10.1--h7e5ed60_0' :
-        'biocontainers/salmon:1.10.1--h7e5ed60_0' }"
+        'https://depot.galaxyproject.org/singularity/salmon:1.10.3--h6dccd9a_2' :
+        'biocontainers/salmon:1.10.3--h6dccd9a_2' }"
 
     input:
     path genome_fasta
diff --git a/modules/nf-core/salmon/index/tests/main.nf.test.snap b/modules/nf-core/salmon/index/tests/main.nf.test.snap
index 703e455c5..e5899b511 100644
--- a/modules/nf-core/salmon/index/tests/main.nf.test.snap
+++ b/modules/nf-core/salmon/index/tests/main.nf.test.snap
@@ -2,25 +2,25 @@
     "versions": {
         "content": [
             [
-                "versions.yml:md5,563eeafb4577be0b13801d7021c0bf42"
+                "versions.yml:md5,85337fa0a286ea35073ee5260974e307"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2023-11-22T14:26:33.32036"
+        "timestamp": "2024-10-18T10:00:47.087293189"
     },
     "versions stub": {
         "content": [
             [
-                "versions.yml:md5,563eeafb4577be0b13801d7021c0bf42"
+                "versions.yml:md5,85337fa0a286ea35073ee5260974e307"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-04-23T09:47:58.828124"
+        "timestamp": "2024-10-18T10:01:03.89824494"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index f1e3b5cda..e2d273525 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -29,7 +29,8 @@ process SALMON_QUANT {
     prefix   = task.ext.prefix ?: "${meta.id}"
 
     def reference   = "--index $index"
-    def reads1 = [], reads2 = []
+    def reads1 = []
+    def reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def input_reads = meta.single_end ? "-r ${reads1.join(" ")}" : "-1 ${reads1.join(" ")} -2 ${reads2.join(" ")}"
     if (alignment_mode) {
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index acfd9252c..caf3c61a8 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -12,11 +12,11 @@ process SAMTOOLS_SORT {
     tuple val(meta2), path(fasta)
 
     output:
-    tuple val(meta), path("*.bam"),     emit: bam,  optional: true
-    tuple val(meta), path("*.cram"),    emit: cram, optional: true
-    tuple val(meta), path("*.crai"),    emit: crai, optional: true
-    tuple val(meta), path("*.csi"),     emit: csi,  optional: true
-    path  "versions.yml"          , emit: versions
+    tuple val(meta), path("*.bam"),  emit: bam,  optional: true
+    tuple val(meta), path("*.cram"), emit: cram, optional: true
+    tuple val(meta), path("*.crai"), emit: crai, optional: true
+    tuple val(meta), path("*.csi"),  emit: csi,  optional: true
+    path  "versions.yml",            emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test
index c2ea9c72a..b05e6691b 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test
@@ -39,6 +39,40 @@ nextflow_process {
         }
     }
 
+    test("multiple bam") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                        process.out.versions
+                ).match()}
+            )
+        }
+    }
+
     test("cram") {
 
         config "./nextflow_cram.config"
@@ -98,6 +132,36 @@ nextflow_process {
         }
     }
 
+    test("multiple bam - stub") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
     test("cram - stub") {
 
         options "-stub"
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
index 2d6b29004..469891fe3 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -159,6 +159,101 @@
         },
         "timestamp": "2024-09-16T08:50:19.061912443"
     },
+    "multiple bam": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.csi"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.09.0"
+        },
+        "timestamp": "2024-10-08T11:59:55.479443"
+    },
+    "multiple bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,d185916eaff9afeb4d0aeab3310371f9"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                    ]
+                ],
+                "crai": [
+                    
+                ],
+                "cram": [
+                    
+                ],
+                "csi": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.sorted.bam.csi:md5,d185916eaff9afeb4d0aeab3310371f9"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.09.0"
+        },
+        "timestamp": "2024-10-08T11:36:13.781404"
+    },
     "bam": {
         "content": [
             [
@@ -185,8 +280,8 @@
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nextflow": "24.09.0"
         },
-        "timestamp": "2024-09-16T08:49:43.971158333"
+        "timestamp": "2024-10-08T11:59:46.372244"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml
index 62054fc97..02cda6e6a 100644
--- a/modules/nf-core/samtools/view/environment.yml
+++ b/modules/nf-core/samtools/view/environment.yml
@@ -4,5 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
+  # renovate: datasource=conda depName=bioconda/htslib
   - bioconda::htslib=1.21
+  # renovate: datasource=conda depName=bioconda/samtools
   - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
index 37e05cec8..41fa3d6a0 100644
--- a/modules/nf-core/samtools/view/main.nf
+++ b/modules/nf-core/samtools/view/main.nf
@@ -4,8 +4,8 @@ process SAMTOOLS_VIEW {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9e/9edc2564215d5cd137a8b25ca8a311600987186d406b092022444adf3c4447f7/data' :
+        'community​.wave​.seqera​.io/library/htslib_samtools:1​.21--6cb89bfd40cbaabf' }"
 
     input:
     tuple val(meta), path(input), path(index)
diff --git a/modules/nf-core/star/align/environment.yml b/modules/nf-core/star/align/environment.yml
index 1debc4c9b..7c57530a3 100644
--- a/modules/nf-core/star/align/environment.yml
+++ b/modules/nf-core/star/align/environment.yml
@@ -3,7 +3,7 @@ channels:
   - bioconda
 
 dependencies:
-  - bioconda::htslib=1.18
-  - bioconda::samtools=1.18
-  - bioconda::star=2.7.10a
+  - bioconda::htslib=1.20
+  - bioconda::samtools=1.20
+  - bioconda::star=2.7.11b
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index b5bc9ddf8..417071ba9 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -4,8 +4,8 @@ process STAR_ALIGN {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' :
-        'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b4/b425bc2a95806d878993f9a66dae3ae80ac2dafff4c208c5ae01b7a90a32fa91/data' :
+        'community.wave.seqera.io/library/star_samtools_htslib_gawk:10c6e8c834460019' }"
 
     input:
     tuple val(meta), path(reads, stageAs: "input*/*")
diff --git a/modules/nf-core/star/align/meta.yml b/modules/nf-core/star/align/meta.yml
index d9cba2aa7..5cfe763e3 100644
--- a/modules/nf-core/star/align/meta.yml
+++ b/modules/nf-core/star/align/meta.yml
@@ -56,27 +56,30 @@ input:
 output:
   - log_final:
       - meta:
-          type: file
-          description: STAR final log file
-          pattern: "*Log.final.out"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*Log.final.out":
           type: file
           description: STAR final log file
           pattern: "*Log.final.out"
   - log_out:
       - meta:
-          type: file
-          description: STAR lot out file
-          pattern: "*Log.out"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*Log.out":
           type: file
           description: STAR lot out file
           pattern: "*Log.out"
   - log_progress:
       - meta:
-          type: file
-          description: STAR log progress file
-          pattern: "*Log.progress.out"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*Log.progress.out":
           type: file
           description: STAR log progress file
@@ -88,57 +91,70 @@ output:
           pattern: "versions.yml"
   - bam:
       - meta:
-          type: file
-          description: Output BAM file containing read alignments
-          pattern: "*.{bam}"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*d.out.bam":
           type: file
           description: Output BAM file containing read alignments
           pattern: "*.{bam}"
   - bam_sorted:
       - meta:
-          type: file
-          description: Sorted BAM file of read alignments (optional)
-          pattern: "*sortedByCoord.out.bam"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - ${prefix}.sortedByCoord.out.bam:
           type: file
           description: Sorted BAM file of read alignments (optional)
           pattern: "*sortedByCoord.out.bam"
   - bam_sorted_aligned:
-      - meta: {}
-      - ${prefix}.Aligned.sortedByCoord.out.bam: {}
-  - bam_transcript:
       - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - ${prefix}.Aligned.sortedByCoord.out.bam:
           type: file
-          description: Output BAM file of transcriptome alignment (optional)
-          pattern: "*toTranscriptome.out.bam"
+          description: Sorted BAM file of read alignments (optional)
+          pattern: "*.Aligned.sortedByCoord.out.bam"
+  - bam_transcript:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*toTranscriptome.out.bam":
           type: file
           description: Output BAM file of transcriptome alignment (optional)
           pattern: "*toTranscriptome.out.bam"
   - bam_unsorted:
       - meta:
-          type: file
-          description: Unsorted BAM file of read alignments (optional)
-          pattern: "*Aligned.unsort.out.bam"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*Aligned.unsort.out.bam":
           type: file
           description: Unsorted BAM file of read alignments (optional)
           pattern: "*Aligned.unsort.out.bam"
   - fastq:
       - meta:
-          type: file
-          description: Unmapped FastQ files (optional)
-          pattern: "*fastq.gz"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*fastq.gz":
           type: file
           description: Unmapped FastQ files (optional)
           pattern: "*fastq.gz"
   - tab:
       - meta:
-          type: file
-          description: STAR output tab file(s) (optional)
-          pattern: "*.tab"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*.tab":
           type: file
           description: STAR output tab file(s) (optional)
@@ -149,12 +165,9 @@ output:
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*.SJ.out.tab"
       - "*.SJ.out.tab":
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
+          type: file
+          description: STAR output splice junction tab file
           pattern: "*.SJ.out.tab"
   - read_per_gene_tab:
       - meta:
@@ -162,18 +175,16 @@ output:
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*.ReadsPerGene.out.tab"
       - "*.ReadsPerGene.out.tab":
+          type: file
+          description: STAR output read per gene tab file
+          pattern: "*.ReadsPerGene.out.tab"
+  - junction:
+      - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*.ReadsPerGene.out.tab"
-  - junction:
-      - meta:
-          type: file
-          description: STAR chimeric junction output file (optional)
-          pattern: "*.out.junction"
       - "*.out.junction":
           type: file
           description: STAR chimeric junction output file (optional)
@@ -186,25 +197,25 @@ output:
             e.g. [ id:'test', single_end:false ]
           pattern: "*.out.sam"
       - "*.out.sam":
+          type: file
+          description: STAR output SAM file(s) (optional)
+          pattern: "*.out.sam"
+  - wig:
+      - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*.out.sam"
-  - wig:
-      - meta:
-          type: file
-          description: STAR output wiggle format file(s) (optional)
-          pattern: "*.wig"
       - "*.wig":
           type: file
           description: STAR output wiggle format file(s) (optional)
           pattern: "*.wig"
   - bedgraph:
       - meta:
-          type: file
-          description: STAR output bedGraph format file(s) (optional)
-          pattern: "*.bg"
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*.bg":
           type: file
           description: STAR output bedGraph format file(s) (optional)
diff --git a/modules/nf-core/star/align/tests/main.nf.test b/modules/nf-core/star/align/tests/main.nf.test
index 2d9f72ddc..a62c17db0 100644
--- a/modules/nf-core/star/align/tests/main.nf.test
+++ b/modules/nf-core/star/align/tests/main.nf.test
@@ -56,13 +56,10 @@ nextflow_process {
                     file(process.out.log_final[0][1]).name,
                     file(process.out.log_out[0][1]).name,
                     file(process.out.log_progress[0][1]).name,
-                    process.out.bam,
-                    process.out.bam_sorted,
-                    process.out.bam_transcript,
-                    process.out.bam_unsorted,
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    bam(process.out.bam_sorted_aligned[0][1]).getReadsMD5(),
                     process.out.bedgraph,
                     process.out.fastq,
-                    process.out.junction,
                     process.out.read_per_gene_tab,
                     process.out.sam,
                     process.out.spl_junc_tab,
@@ -124,13 +121,10 @@ nextflow_process {
                     file(process.out.log_final[0][1]).name,
                     file(process.out.log_out[0][1]).name,
                     file(process.out.log_progress[0][1]).name,
-                    process.out.bam,
-                    process.out.bam_sorted,
-                    process.out.bam_transcript,
-                    process.out.bam_unsorted,
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    bam(process.out.bam_sorted_aligned[0][1]).getReadsMD5(),
                     process.out.bedgraph,
                     process.out.fastq,
-                    process.out.junction,
                     process.out.read_per_gene_tab,
                     process.out.sam,
                     process.out.spl_junc_tab,
@@ -192,13 +186,9 @@ nextflow_process {
                     file(process.out.log_final[0][1]).name,
                     file(process.out.log_out[0][1]).name,
                     file(process.out.log_progress[0][1]).name,
-                    process.out.bam,
-                    process.out.bam_sorted,
-                    process.out.bam_transcript,
-                    process.out.bam_unsorted,
+                    bam(process.out.bam[0][1]).getReadsMD5(),
                     process.out.bedgraph,
                     process.out.fastq,
-                    process.out.junction,
                     process.out.read_per_gene_tab,
                     process.out.sam,
                     process.out.spl_junc_tab,
@@ -260,13 +250,10 @@ nextflow_process {
                     file(process.out.log_final[0][1]).name,
                     file(process.out.log_out[0][1]).name,
                     file(process.out.log_progress[0][1]).name,
-                    process.out.bam,
-                    process.out.bam_sorted,
-                    process.out.bam_transcript,
-                    process.out.bam_unsorted,
+                    file(process.out.junction[0][1]).name,
+                    bam(process.out.bam[0][1]).getReadsMD5(),
                     process.out.bedgraph,
                     process.out.fastq,
-                    process.out.junction,
                     process.out.read_per_gene_tab,
                     process.out.sam,
                     process.out.spl_junc_tab,
@@ -330,13 +317,10 @@ nextflow_process {
                     file(process.out.log_final[0][1]).name,
                     file(process.out.log_out[0][1]).name,
                     file(process.out.log_progress[0][1]).name,
-                    process.out.bam,
-                    process.out.bam_sorted,
-                    process.out.bam_transcript,
-                    process.out.bam_unsorted,
+                    bam(process.out.bam[0][1]).getReadsMD5(),
+                    bam(process.out.bam_sorted_aligned[0][1]).getReadsMD5(),
                     process.out.bedgraph,
                     process.out.fastq,
-                    process.out.junction,
                     process.out.read_per_gene_tab,
                     process.out.sam,
                     process.out.spl_junc_tab,
@@ -606,4 +590,4 @@ nextflow_process {
             )
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/star/align/tests/main.nf.test.snap b/modules/nf-core/star/align/tests/main.nf.test.snap
index 0da28d37c..b533fb8b4 100644
--- a/modules/nf-core/star/align/tests/main.nf.test.snap
+++ b/modules/nf-core/star/align/tests/main.nf.test.snap
@@ -97,7 +97,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "4": [
                     [
@@ -307,7 +307,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "wig": [
                     [
@@ -322,9 +322,9 @@
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:39:15.440397"
+        "timestamp": "2024-10-20T17:09:08.738074176"
     },
     "homo_sapiens - paired_end - arriba - stub": {
         "content": [
@@ -424,7 +424,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "4": [
                     [
@@ -634,7 +634,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "wig": [
                     [
@@ -649,33 +649,17 @@
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:40:15.472109"
+        "timestamp": "2024-10-20T17:09:36.122131869"
     },
     "homo_sapiens - single_end": {
         "content": [
             "test.Log.final.out",
             "test.Log.out",
             "test.Log.progress.out",
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": true
-                    },
-                    "test.Aligned.sortedByCoord.out.bam:md5,c6cfaccaf91bc7fdabed3cfe236d4535"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
+            "9f76be49a6607613a64f760101bdddce",
+            "9f76be49a6607613a64f760101bdddce",
             [
                 [
                     {
@@ -696,9 +680,6 @@
             ],
             [
                 
-            ],
-            [
-                
             ],
             [
                 [
@@ -722,38 +703,22 @@
                 
             ],
             [
-                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:23:08.001162"
+        "timestamp": "2024-10-20T17:01:22.197991909"
     },
     "homo_sapiens - paired_end": {
         "content": [
             "test.Log.final.out",
             "test.Log.out",
             "test.Log.progress.out",
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Aligned.sortedByCoord.out.bam:md5,b9ee1c607e07323bc1652ef3babb543f"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
+            "db9a8324b5163b025bcc0c33e848486",
+            "db9a8324b5163b025bcc0c33e848486",
             [
                 [
                     {
@@ -774,9 +739,6 @@
             ],
             [
                 
-            ],
-            [
-                
             ],
             [
                 [
@@ -800,14 +762,14 @@
                 
             ],
             [
-                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:24:46.295219"
+        "timestamp": "2024-10-20T17:02:06.988663857"
     },
     "homo_sapiens - paired_end - multiple - stub": {
         "content": [
@@ -907,7 +869,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "4": [
                     [
@@ -1117,7 +1079,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "wig": [
                     [
@@ -1132,33 +1094,17 @@
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:41:07.310866"
+        "timestamp": "2024-10-20T17:10:12.005468781"
     },
     "homo_sapiens - paired_end - multiple": {
         "content": [
             "test.Log.final.out",
             "test.Log.out",
             "test.Log.progress.out",
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Aligned.sortedByCoord.out.bam:md5,ab07c21d63ab0a6c07d171d213c81d5a"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
+            "3e54e45f5dc3e9c1f2fc55bc41531a87",
+            "3e54e45f5dc3e9c1f2fc55bc41531a87",
             [
                 [
                     {
@@ -1179,9 +1125,6 @@
             ],
             [
                 
-            ],
-            [
-                
             ],
             [
                 [
@@ -1205,14 +1148,14 @@
                 
             ],
             [
-                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:38:46.605044"
+        "timestamp": "2024-10-20T17:08:54.877286681"
     },
     "homo_sapiens - paired_end - stub": {
         "content": [
@@ -1312,7 +1255,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "4": [
                     [
@@ -1522,7 +1465,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "wig": [
                     [
@@ -1537,24 +1480,17 @@
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:39:51.595873"
+        "timestamp": "2024-10-20T17:09:20.911466345"
     },
     "homo_sapiens - paired_end - starfusion": {
         "content": [
             "test.Log.final.out",
             "test.Log.out",
             "test.Log.progress.out",
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Aligned.out.bam:md5,bcad07b838f6762fc01eea52b5cd3f84"
-                ]
-            ],
+            "test.Chimeric.out.junction",
+            "caee9dcda13882d4913456973c25b57a",
             [
                 
             ],
@@ -1566,24 +1502,6 @@
             ],
             [
                 
-            ],
-            [
-                
-            ],
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Chimeric.out.junction:md5,c10ef219f4a30e83711b995bc5e40dba"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                
             ],
             [
                 [
@@ -1607,41 +1525,21 @@
                 
             ],
             [
-                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:35:43.874508"
+        "timestamp": "2024-10-20T17:07:25.0639914"
     },
     "homo_sapiens - paired_end - arriba": {
         "content": [
             "test.Log.final.out",
             "test.Log.out",
             "test.Log.progress.out",
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.Aligned.out.bam:md5,c1b1747f5873f2d17762725636e891d5"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
+            "1a3abe88fb2490589c58497d39921bcc",
             [
                 
             ],
@@ -1676,14 +1574,14 @@
                 
             ],
             [
-                "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:28:32.599223"
+        "timestamp": "2024-10-20T17:04:00.685784211"
     },
     "homo_sapiens - paired_end - starfusion - stub": {
         "content": [
@@ -1783,7 +1681,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "4": [
                     [
@@ -1993,7 +1891,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,2e6b6d8809f5a17f38f4d27c45dcb22f"
+                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
                 ],
                 "wig": [
                     [
@@ -2008,8 +1906,8 @@
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-10-01T11:40:42.29249"
+        "timestamp": "2024-10-20T17:09:53.173671551"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/star/genomegenerate/environment.yml b/modules/nf-core/star/genomegenerate/environment.yml
index 1debc4c9b..7c57530a3 100644
--- a/modules/nf-core/star/genomegenerate/environment.yml
+++ b/modules/nf-core/star/genomegenerate/environment.yml
@@ -3,7 +3,7 @@ channels:
   - bioconda
 
 dependencies:
-  - bioconda::htslib=1.18
-  - bioconda::samtools=1.18
-  - bioconda::star=2.7.10a
+  - bioconda::htslib=1.20
+  - bioconda::samtools=1.20
+  - bioconda::star=2.7.11b
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/genomegenerate/main.nf b/modules/nf-core/star/genomegenerate/main.nf
index b8855715b..8f0c67e76 100644
--- a/modules/nf-core/star/genomegenerate/main.nf
+++ b/modules/nf-core/star/genomegenerate/main.nf
@@ -4,8 +4,8 @@ process STAR_GENOMEGENERATE {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' :
-        'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:ded3841da0194af2701c780e9b3d653a85d27489-0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b4/b425bc2a95806d878993f9a66dae3ae80ac2dafff4c208c5ae01b7a90a32fa91/data' :
+        'community.wave.seqera.io/library/star_samtools_htslib_gawk:10c6e8c834460019' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
index 207f4b4f5..3db256782 100644
--- a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
+++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
@@ -3,14 +3,14 @@
         "content": [
             "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]",
             [
-                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T14:55:35.478401"
+        "timestamp": "2024-10-19T20:37:47.410432728"
     },
     "fasta_gtf_stub": {
         "content": [
@@ -41,7 +41,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
                 ],
                 "index": [
                     [
@@ -69,15 +69,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T14:55:57.247585"
+        "timestamp": "2024-10-19T20:38:09.165234795"
     },
     "fasta_stub": {
         "content": [
@@ -101,7 +101,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
                 ],
                 "index": [
                     [
@@ -122,27 +122,27 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T14:56:07.01742"
+        "timestamp": "2024-10-19T20:38:19.530862664"
     },
     "fasta": {
         "content": [
             "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]",
             [
-                "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f"
+                "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T14:55:45.48784"
+        "timestamp": "2024-10-19T20:37:58.667436398"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/stringtie/stringtie/environment.yml b/modules/nf-core/stringtie/stringtie/environment.yml
index 0556de41a..906b7486c 100644
--- a/modules/nf-core/stringtie/stringtie/environment.yml
+++ b/modules/nf-core/stringtie/stringtie/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::stringtie=2.2.1
+  - bioconda::stringtie=2.2.3
diff --git a/modules/nf-core/stringtie/stringtie/main.nf b/modules/nf-core/stringtie/stringtie/main.nf
index 6e25ba27d..4635c8c5b 100644
--- a/modules/nf-core/stringtie/stringtie/main.nf
+++ b/modules/nf-core/stringtie/stringtie/main.nf
@@ -4,8 +4,8 @@ process STRINGTIE_STRINGTIE {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/stringtie:2.2.1--hecb563c_2' :
-        'biocontainers/stringtie:2.2.1--hecb563c_2' }"
+        'https://depot.galaxyproject.org/singularity/stringtie:2.2.3--h43eeafb_0' :
+        'biocontainers/stringtie:2.2.3--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap b/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
index 124dd4cbe..d4645de3e 100644
--- a/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
+++ b/modules/nf-core/stringtie/stringtie/tests/main.nf.test.snap
@@ -31,18 +31,18 @@
                         "id": "test",
                         "strandedness": "forward"
                     },
-                    "test.transcripts.gtf:md5,f56cf8aba2c4a5673bc7963ba5f12d04"
+                    "test.transcripts.gtf:md5,37154e7bda96544f24506ee902bb561d"
                 ]
             ],
             [
-                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:33:44.299962"
+        "timestamp": "2024-10-18T09:56:50.294157199"
     },
     "sarscov2 [bam] - forward strandedness": {
         "content": [
@@ -61,18 +61,18 @@
                         "id": "test",
                         "strandedness": "forward"
                     },
-                    "test.transcripts.gtf:md5,569137af5be452413086b50653a97203"
+                    "test.transcripts.gtf:md5,6087dfc9700a52d9e4a1ae3fcd1d1dfd"
                 ]
             ],
             [
-                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:33:35.177738"
+        "timestamp": "2024-10-18T09:56:39.4249133"
     },
     "sarscov2 [bam] - forward strandedness - stub": {
         "content": [
@@ -114,7 +114,7 @@
                     ]
                 ],
                 "4": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ],
                 "abundance": [
                     [
@@ -153,15 +153,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:36:32.885078"
+        "timestamp": "2024-10-18T09:57:23.008470065"
     },
     "sarscov2 [bam] - forward strandedness + reference annotation - stub": {
         "content": [
@@ -203,7 +203,7 @@
                     ]
                 ],
                 "4": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ],
                 "abundance": [
                     [
@@ -242,15 +242,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:36:43.325777"
+        "timestamp": "2024-10-18T09:57:33.622824981"
     },
     "sarscov2 [bam] - reverse strandedness + reference annotation - stub": {
         "content": [
@@ -292,7 +292,7 @@
                     ]
                 ],
                 "4": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ],
                 "abundance": [
                     [
@@ -331,15 +331,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:37:06.085936"
+        "timestamp": "2024-10-18T09:57:55.803421433"
     },
     "sarscov2 [bam] - reverse strandedness - stub": {
         "content": [
@@ -381,7 +381,7 @@
                     ]
                 ],
                 "4": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ],
                 "abundance": [
                     [
@@ -420,15 +420,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                    "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:36:53.837578"
+        "timestamp": "2024-10-18T09:57:44.825389635"
     },
     "sarscov2 [bam] - reverse strandedness + reference annotation": {
         "content": [
@@ -462,18 +462,18 @@
                         "id": "test",
                         "strandedness": "reverse"
                     },
-                    "test.transcripts.gtf:md5,bb346053a8c156b803b055133376c7fa"
+                    "test.transcripts.gtf:md5,fbabb4e3888bbede67f11f692e484880"
                 ]
             ],
             [
-                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:34:03.114695"
+        "timestamp": "2024-10-18T09:57:11.793664242"
     },
     "sarscov2 [bam] - reverse strandedness": {
         "content": [
@@ -492,17 +492,17 @@
                         "id": "test",
                         "strandedness": "reverse"
                     },
-                    "test.transcripts.gtf:md5,31c34aec2bf36bb0ea3c16c2afeeeb1f"
+                    "test.transcripts.gtf:md5,01d6da00a3c458420841e57427297183"
                 ]
             ],
             [
-                "versions.yml:md5,3410e8ac349d18c85ddee89337851d38"
+                "versions.yml:md5,06593ea00cc35bf06f2de2753e0c3913"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
-            "nextflow": "24.04.3"
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-07-22T12:33:52.874479"
+        "timestamp": "2024-10-18T09:57:01.166309777"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index ce142a749..fd2ec9632 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -11,6 +11,7 @@ workflow ARRIBA_WORKFLOW {
         ch_fasta
         ch_starindex_ref
         ch_arriba_ref_blacklist
+        ch_arriba_ref_cytobands
         ch_arriba_ref_known_fusions
         ch_arriba_ref_protein_domains
 
@@ -28,7 +29,7 @@ workflow ARRIBA_WORKFLOW {
                     .map { meta, reads, fusions -> [ meta, fusions ] }
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
-                ARRIBA_ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, ch_arriba_ref_blacklist, ch_arriba_ref_known_fusions, [[],[]], [[],[]], ch_arriba_ref_protein_domains )
+                ARRIBA_ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, ch_arriba_ref_blacklist, ch_arriba_ref_known_fusions, ch_arriba_ref_cytobands, ch_arriba_ref_protein_domains )
                 ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
                 ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 0c6c99164..3de5af74c 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -35,9 +35,6 @@ include { GFFREAD }                         from '../../modules/nf-core/gffread/
 */
 
 workflow BUILD_REFERENCES {
-    take:
-        genome                           // channel: [mandatory]  val(genome)
-        genome_gencode_version           // channel: [mandatory]  val(genome_gencode_version)
 
     main:
     ch_versions = Channel.empty()
@@ -82,7 +79,7 @@ workflow BUILD_REFERENCES {
 
     if (!file(params.refflat).exists() || file(params.refflat).isEmpty()){
         GTF_TO_REFFLAT(ch_gtf)
-        ch_refflat = GTF_TO_REFFLAT.out.refflat
+        ch_refflat = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
     } else {
         ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
     }
@@ -109,58 +106,55 @@ workflow BUILD_REFERENCES {
             (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
             !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
             !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
-        ARRIBA_DOWNLOAD(genome)
+        ARRIBA_DOWNLOAD(params.genome)
         ch_arriba_ref_blacklist = ARRIBA_DOWNLOAD.out.blacklist
         ch_arriba_ref_cytobands = ARRIBA_DOWNLOAD.out.cytobands
         ch_arriba_ref_known_fusions = ARRIBA_DOWNLOAD.out.known_fusions
         ch_arriba_ref_protein_domains = ARRIBA_DOWNLOAD.out.protein_domains
     } else {
-        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { that -> [[id:that.Name], that] }
-        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { that -> [[id:that.Name], that] }
-        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { that -> [[id:that.Name], that] }
-        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { that -> [[id:that.Name], that] }
+        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist)
+        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands)
+        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions)
+        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
     }
 
 
     if ((params.fusioncatcher || params.all) &&
             (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
             !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
-        if (params.download_refs) {
-            FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
-            ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference}
-        else {
             FUSIONCATCHER_BUILD(params.genome_gencode_version)
-            ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference}
-        } else {
-            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { that -> [[id:that.Name], that] }
-        }
+            ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
+    }
+    else {
+        ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref)
+    }
 
 
     if ((params.starfusion || params.all) &&
             (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
             !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
-        if (params.download_refs) {
-            ch_starfusion_ref = STARFUSION_DOWNLOAD( ch_fasta, ch_gtf ).out.reference }
-        else {
-            ch_starfusion_ref = STARFUSION_BUILD( ch_fasta, ch_gtf ).out.reference }
-    } else {
-        ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { that -> [[id:that.Name], that] }}
+            STARFUSION_BUILD(ch_fasta, ch_gtf)
+            ch_starfusion_ref = STARFUSION_BUILD.out.reference
+    }
+    else {
+        ch_starfusion_ref = Channel.fromPath(params.starfusion_ref)
+    }
 
 
     if ((params.fusionreport || params.all) &&
             (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
             !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
         if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
-        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd ).out.reference
+        FUSIONREPORT_DOWNLOAD(params.cosmic_username, params.cosmic_passwd)
+        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.reference
     } else {
-        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
+        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref)
     }
 
     emit:
     ch_fasta
     ch_gtf
     ch_fai
-
     ch_hgnc_ref
     ch_hgnc_date
     ch_rrna_interval
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index 09ec99657..f8fc90dc0 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -23,7 +23,7 @@ workflow FUSIONREPORT_WORKFLOW {
             FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
             ch_fusion_list = FUSIONREPORT.out.fusion_list
             ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered
-            ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
+            // ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
             ch_report = FUSIONREPORT.out.report
             ch_csv = FUSIONREPORT.out.csv
         } else {
diff --git a/test.fusionreport.tsv b/test.fusionreport.tsv
new file mode 100644
index 000000000..e69de29bb
diff --git a/test.fusionreport_filtered.tsv b/test.fusionreport_filtered.tsv
new file mode 100644
index 000000000..e69de29bb
diff --git a/test.fusions.csv b/test.fusions.csv
new file mode 100644
index 000000000..e69de29bb
diff --git a/test.fusions.json b/test.fusions.json
new file mode 100644
index 000000000..e69de29bb
diff --git a/test_fusionreport_index.html b/test_fusionreport_index.html
new file mode 100644
index 000000000..e69de29bb
diff --git a/tests/main.nf.test b/tests/main.nf.test
index 715335d8f..31c71b6f1 100644
--- a/tests/main.nf.test
+++ b/tests/main.nf.test
@@ -5,11 +5,10 @@ nextflow_pipeline {
     tag "pipeline"
     tag "pipeline_rnafusion"
 
-    test("Run build references with profile test") {
+    test("Run fusion detection with profile test") {
 
         when {
             params {
-                build_references             = true
                 outdir                       = "results"
                 genome_base                  = "references"
                 max_cpus                     = 2
@@ -21,25 +20,6 @@ nextflow_pipeline {
             }
         }
 
-        then {
-            assertAll(
-                { assert workflow.success }
-            )
-        }
-    }
-    test("Run fusion detection with profile test") {
-
-        when {
-            params {
-                outdir                       = "results"
-                genome_base                  = "references"
-                max_cpus                     = 2
-                max_memory                   = '6.GB'
-                max_time                     = '6.h'
-                input                        = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-            }
-        }
-
         then {
             assertAll(
                 { assert workflow.success }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 5e34db974..238bcc3af 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -5,20 +5,20 @@
 */
 
 include { BUILD_REFERENCES              }   from '../subworkflows/local/build_references'
-// include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
-// include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
-// include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
-// include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
-// include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow'
-// include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
-// include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
-// include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
-// include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
+include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
+include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
+include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
+include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
+include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow'
+include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
+include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
+include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
+include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 
 include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
 include { FASTQC                        }   from '../modules/nf-core/fastqc/main'
 include { MULTIQC                       }   from '../modules/nf-core/multiqc/main'
-// include { SALMON_QUANT                  }   from '../modules/nf-core/salmon/quant/main'
+include { SALMON_QUANT                  }   from '../modules/nf-core/salmon/quant/main'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -34,204 +34,123 @@ workflow RNAFUSION {
 
 
     ch_versions = Channel.empty()
-    // ch_multiqc_files = Channel.empty()
+    ch_multiqc_files = Channel.empty()
 
     //
     // Create references if necessary
     //
 
-    BUILD_REFERENCES(params.genome, params.genome_gencode_version)
+    BUILD_REFERENCES()
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
-    // // Optional
-    // ch_fasta                      = params.fasta                        ? Channel.fromPath(params.fasta).map {it -> [[id:it[0].simpleName], it]}.collect()
-    //                                                                     : BUILD_REFERENCES.out.fasta.map {it -> [[id:it[0].simpleName], it]}.collect()
-    // ch_gtf                        = params.gtf                          ? Channel.fromPath(params.gtf).map {it -> [[id:it[0].simpleName], it]}.collect()
-    //                                                                     : downloads.gtf.map {it -> [[id:it[0].simpleName], it]}.collect()
-    // ch_vep_cache_unprocessed      = params.vep_cache                    ? Channel.fromPath(params.vep_cache)
-    //                                                                     : Channel.empty().mix(downloads.vep_cache)
-    // ch_vep_extra_files_unsplit    = params.vep_plugin_files             ? Channel.fromPath(params.vep_plugin_files)
-    //                                                                     : Channel.empty().mix(downloads.vep_plugin)
-    // ch_fai                        = params.fai                          ? Channel.fromPath(params.fai).map {it -> [[id:it[0].simpleName], it]}.collect()
-    //                                                                     : Channel.empty()
-    // ch_gene_panel_clinical_filter = params.gene_panel_clinical_filter   ? Channel.fromPath(params.gene_panel_clinical_filter).collect()
-    //                                                                     : Channel.empty()
-    // ch_ref_drop_annot_file        = params.reference_drop_annot_file    ? Channel.fromPath(params.reference_drop_annot_file).collect()
-    //                                                                     : Channel.empty()
-    // ch_ref_drop_count_file        = params.reference_drop_count_file    ? Channel.fromPath(params.reference_drop_count_file).collect()
-    //                                                                     : Channel.empty()
-    // ch_ref_drop_splice_folder     = params.reference_drop_splice_folder ? Channel.fromPath(params.reference_drop_splice_folder).collect()
-    //                                                                     : Channel.empty()
-    // ch_salmon_index               = params.salmon_index                 ? Channel.fromPath(params.salmon_index)
-    //                                                                     : Channel.empty()
-    // ch_star_index                 = params.star_index                   ? Channel.fromPath(params.star_index).map {it -> [[id:it[0].simpleName], it]}.collect()
-    //                                                                     : Channel.empty()
-    // ch_transcript_fasta           = params.transcript_fasta             ? Channel.fromPath(params.transcript_fasta)
-    //                                                                     : Channel.empty()
-    // ch_sequence_dict              = params.sequence_dict                ? Channel.fromPath(params.sequence_dict).map{ it -> [[id:it[0].simpleName], it] }.collect()
-    //                                                                     : Channel.empty()
-    // ch_subsample_bed              = params.subsample_bed                ? Channel.fromPath(params.subsample_bed).collect()
-    //                                                                     : Channel.empty()
 
     //
-    // Create channel from input file provided through params.input
+    // QC from FASTQ files
     //
-    // Channel
-    //     .fromSamplesheet("input")
-    //     .map {
-    //         meta, fastq_1, fastq_2, strandedness ->
-    //             if (!fastq_2) {
-    //                 return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ]
-    //             } else {
-    //                 return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ]
-    //             }
-    //     }
-    //     .groupTuple()
-    //     .map {
-    //         validateInputSamplesheet(it)
-    //     }
-    //     .branch {
-    //         meta, fastqs ->
-    //             single  : fastqs.size() == 1
-    //                 return [ meta, fastqs.flatten() ]
-    //             multiple: fastqs.size() > 1
-    //                 return [ meta, fastqs.flatten() ]
-    //     }
-    //     .set { ch_fastq }
+    FASTQC (
+        ch_samplesheet
+    )
+    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+    ch_versions = ch_versions.mix(FASTQC.out.versions)
 
-    // //
-    // // MODULE: Concatenate FastQ files from same sample if required
-    // //
-    // CAT_FASTQ (
-    //     ch_fastq.multiple
-    // )
-    // .reads
-    // .mix(ch_fastq.single)
-    // .set { ch_cat_fastq }
-    // ch_versions = ch_versions.mix(CAT_FASTQ.out.versions)
-
-
-    // //
-    // // QC from FASTQ files
-    // //
-    // FASTQC (
-    //     ch_cat_fastq
-    // )
-    // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    // ch_versions = ch_versions.mix(FASTQC.out.versions)
 
-    // SALMON_QUANT( ch_reads_all, BUILD_REFERENCES.out.ch_salmon_index.map{ meta, index ->  index  }, BUILD_REFERENCES.out.ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
-    // ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.zip.collect{it[1]})
+    //
+    // Trimming
+    //
+    TRIM_WORKFLOW (
+        ch_samplesheet
+    )
+    ch_reads = TRIM_WORKFLOW.out.trimmed_reads
+    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+
+    // SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ meta, index ->  index  }, BUILD_REFERENCES.out.ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
+    // ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.zi.collect{it[1]})
     // ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
 
-    // //
-    // // Trimming
-    // //
-    // TRIM_WORKFLOW (
-    //     ch_cat_fastq
-    // )
-    // ch_reads = TRIM_WORKFLOW.out.trimmed_reads
-    // ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+
     //
-    // MODULE: Run FastQC
+    // SUBWORKFLOW:  Run STAR alignment and Arriba
     //
-    // FASTQC (
-    //     ch_samplesheet
-    // )
-    // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    // ch_versions = ch_versions.mix(FASTQC.out.versions.first())
 
+    ARRIBA_WORKFLOW (
+        ch_reads,
+        BUILD_REFERENCES.out.ch_gtf,
+        BUILD_REFERENCES.out.ch_fasta,
+        BUILD_REFERENCES.out.ch_starindex_ref,
+        BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
+        BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
+        BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
+        BUILD_REFERENCES.out.ch_arriba_ref_protein_domains
+    )
+    ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
+
+
+//Run STAR fusion
+    STARFUSION_WORKFLOW (
+        ch_reads,
+        BUILD_REFERENCES.out.ch_gtf,
+        BUILD_REFERENCES.out.ch_starindex_ref,
+        BUILD_REFERENCES.out.ch_fasta
+    )
+    ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+
+
+//Run fusioncatcher
+    FUSIONCATCHER_WORKFLOW (
+        ch_reads
+    )
+    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+
+
+//Run stringtie
+    STRINGTIE_WORKFLOW (
+        STARFUSION_WORKFLOW.out.ch_bam_sorted,
+        BUILD_REFERENCES.out.ch_gtf
+    )
+    ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
+
+
+    //Run fusion-report
+    FUSIONREPORT_WORKFLOW (
+        ch_reads,
+        BUILD_REFERENCES.out.ch_fusionreport_ref,
+        ARRIBA_WORKFLOW.out.fusions,
+        STARFUSION_WORKFLOW.out.fusions,
+        FUSIONCATCHER_WORKFLOW.out.fusions
+    )
+    ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
+
+
+
+    //Run fusionInpector
+    FUSIONINSPECTOR_WORKFLOW (
+        ch_reads,
+        FUSIONREPORT_WORKFLOW.out.fusion_list,
+        FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
+        FUSIONREPORT_WORKFLOW.out.report,
+        FUSIONREPORT_WORKFLOW.out.csv,
+        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+        BUILD_REFERENCES.out.ch_gtf,
+        BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
+        BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
+        BUILD_REFERENCES.out.ch_hgnc_ref,
+        BUILD_REFERENCES.out.ch_hgnc_date
+    )
+    ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
 
 
-    // TRIM_WORKFLOW (
-    //     ch_samplesheet
+    // //QC
+    // QC_WORKFLOW (
+    //     ch_reads,
+    //     STARFUSION_WORKFLOW.out.ch_bam_sorted,
+    //     STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+    //     BUILD_REFERENCES.out.ch_gtf,
+    //     BUILD_REFERENCES.out.ch_refflat,
+    //     BUILD_REFERENCES.out.ch_fasta,
+    //     BUILD_REFERENCES.out.ch_fai,
+    //     BUILD_REFERENCES.out.ch_rrna_interval
     // )
-    // ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
-    // ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
-    // ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
-
-
-//     //
-//     // SUBWORKFLOW:  Run STAR alignment and Arriba
-//     //
-//     ARRIBA_WORKFLOW (
-//         ch_reads_all,
-//         ch_gtf,
-//         ch_fasta,
-//         ch_starindex_ensembl_ref,
-//         ch_arriba_ref_blacklist,
-//         ch_arriba_ref_known_fusions,
-//         ch_arriba_ref_protein_domains
-//     )
-//     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
-
-
-// //Run STAR fusion
-//     STARFUSION_WORKFLOW (
-//         ch_reads_all,
-//         ch_gtf,
-//         ch_starindex_ref,
-//         ch_fasta
-//     )
-//     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
-
-
-// //Run fusioncatcher
-//     FUSIONCATCHER_WORKFLOW (
-//         ch_reads_fusioncatcher
-//     )
-//     ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
-
-
-// //Run stringtie
-//     STRINGTIE_WORKFLOW (
-//         STARFUSION_WORKFLOW.out.ch_bam_sorted,
-//         ch_gtf
-//     )
-//     ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
-
-
-//     //Run fusion-report
-//     FUSIONREPORT_WORKFLOW (
-//         ch_reads_all,
-//         ch_fusionreport_ref,
-//         ARRIBA_WORKFLOW.out.fusions,
-//         STARFUSION_WORKFLOW.out.fusions,
-//         FUSIONCATCHER_WORKFLOW.out.fusions
-//     )
-//     ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
-
-
-
-//     //Run fusionInpector
-//     FUSIONINSPECTOR_WORKFLOW (
-//         ch_reads_all,
-//         FUSIONREPORT_WORKFLOW.out.fusion_list,
-//         FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
-//         FUSIONREPORT_WORKFLOW.out.report,
-//         FUSIONREPORT_WORKFLOW.out.csv,
-//         STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-//         ch_gtf,
-//         ch_arriba_ref_protein_domains,
-//         ch_arriba_ref_cytobands,
-//         ch_hgnc_ref,
-//         ch_hgnc_date
-//     )
-//     ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
-
-
-//     //QC
-//     QC_WORKFLOW (
-//         ch_reads_all,
-//         STARFUSION_WORKFLOW.out.ch_bam_sorted,
-//         STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-//         ch_gtf,
-//         ch_refflat,
-//         ch_fasta,
-//         ch_fai,
-//         ch_rrna_interval
-//     )
-//     ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
+    // ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
 
 //     //
 //     // Collate and save software versions
@@ -244,18 +163,6 @@ workflow RNAFUSION {
 //             newLine: true
 //         ).set { ch_collated_versions }
 
-    // //QC
-    // QC_WORKFLOW (
-    //     ch_reads_all,
-    //     STARFUSION_WORKFLOW.out.ch_bam_sorted,
-    //     ch_gtf,
-    //     ch_refflat,
-    //     ch_fasta,
-    //     ch_fai,
-    //     ch_rrna_interval
-    // )
-    // ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
-
 
     // //
     // // Collate and save software versions

From 786b9bf4ec84fd6e2571ddd034b9923151cd4128 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 14 Nov 2024 15:45:31 +0100
Subject: [PATCH 080/887] remove unnecessary file

---
 AAA_BBB.html | 0
 1 file changed, 0 insertions(+), 0 deletions(-)
 delete mode 100644 AAA_BBB.html

diff --git a/AAA_BBB.html b/AAA_BBB.html
deleted file mode 100644
index e69de29bb..000000000

From 537b8410800e61cc464b039e7b73e709a603a022 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 26 Nov 2024 14:17:18 +0000
Subject: [PATCH 081/887] test: ignore nf-test files

---
 .gitignore | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.gitignore b/.gitignore
index a42ce0162..23b0c7de9 100644
--- a/.gitignore
+++ b/.gitignore
@@ -7,3 +7,4 @@ testing/
 testing*
 *.pyc
 null/
+.nf-test*

From 49bc38f761f9125dfe32355734ff65e3a906f516 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 26 Nov 2024 14:17:45 +0000
Subject: [PATCH 082/887] test: create nf-test for local module ensembl
 download

---
 modules/local/ensembl/tests/main.nf.test      | 37 +++++++++++++++++++
 modules/local/ensembl/tests/main.nf.test.snap | 17 +++++++++
 2 files changed, 54 insertions(+)
 create mode 100644 modules/local/ensembl/tests/main.nf.test
 create mode 100644 modules/local/ensembl/tests/main.nf.test.snap

diff --git a/modules/local/ensembl/tests/main.nf.test b/modules/local/ensembl/tests/main.nf.test
new file mode 100644
index 000000000..e30a826f8
--- /dev/null
+++ b/modules/local/ensembl/tests/main.nf.test
@@ -0,0 +1,37 @@
+nextflow_process {
+
+    name "Test Process ENSEMBL_DOWNLOAD"
+    script "../main.nf"
+    process "ENSEMBL_DOWNLOAD"
+
+    test("Should download Ensembl files") {
+
+        when {
+            params {
+                outdir = "tests/results"
+                genome = "GRCh38"
+            }
+            process {
+                """
+                input[0] = "102"
+                input[1] = "GRCh38"
+                input[2] = "GRCh38"
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.gtf[0][1]).name,
+                    file(process.out.primary_assembly[0][1]).name,
+                    file(process.out.chrgtf[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/local/ensembl/tests/main.nf.test.snap b/modules/local/ensembl/tests/main.nf.test.snap
new file mode 100644
index 000000000..a6eb2971d
--- /dev/null
+++ b/modules/local/ensembl/tests/main.nf.test.snap
@@ -0,0 +1,17 @@
+{
+    "Should download Ensembl files": {
+        "content": [
+            "Homo_sapiens.GRCh38.102.gtf",
+            "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
+            "Homo_sapiens.GRCh38.102.chr.gtf",
+            [
+                "versions.yml:md5,efb55665a151fe2b4d9eac70c0e01bb0"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-26T14:06:24.319131837"
+    }
+}
\ No newline at end of file

From f767168d2658127fbbd0c362ccab0c08246a2f94 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 26 Nov 2024 14:20:59 +0000
Subject: [PATCH 083/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7875d141e..40792bdb6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Normalized gene expression calculated [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Primary assembly now used as main reference genome FASTA file, as recommended by the STAR manual [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Use of only ensembl GTF file, not chr.gtf file as GTF reference file [#488](https://github.com/nf-core/rnafusion/pull/488)
+- Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#508](https://github.com/nf-core/rnafusion/pull/508)
 
 ### Changed
 

From 99fc80d7fb6cb83c67c35d2c04a9a2b787704b34 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 26 Nov 2024 16:13:00 +0000
Subject: [PATCH 084/887] test: create nf-tests for local `HGNC_DOWNLOAD`
 module

---
 modules/local/hgnc/tests/main.nf.test      | 52 ++++++++++++++++++++++
 modules/local/hgnc/tests/main.nf.test.snap | 16 +++++++
 2 files changed, 68 insertions(+)
 create mode 100644 modules/local/hgnc/tests/main.nf.test
 create mode 100644 modules/local/hgnc/tests/main.nf.test.snap

diff --git a/modules/local/hgnc/tests/main.nf.test b/modules/local/hgnc/tests/main.nf.test
new file mode 100644
index 000000000..6f4c03d05
--- /dev/null
+++ b/modules/local/hgnc/tests/main.nf.test
@@ -0,0 +1,52 @@
+nextflow_process {
+
+    name "Test Process HGNC_DOWNLOAD"
+    script "../main.nf"
+    process "HGNC_DOWNLOAD"
+
+    test("Should download HGNC files") {
+
+        when {
+            process {
+                """
+                // This process doesn't have any inputs
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.hgnc_ref[0]).name,
+                    file(process.out.hgnc_date[0]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("Should create stub files") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                // This process doesn't have any inputs
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.hgnc_ref[0]).name,
+                    file(process.out.hgnc_date[0]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/modules/local/hgnc/tests/main.nf.test.snap b/modules/local/hgnc/tests/main.nf.test.snap
new file mode 100644
index 000000000..856fe2509
--- /dev/null
+++ b/modules/local/hgnc/tests/main.nf.test.snap
@@ -0,0 +1,16 @@
+{
+    "Should create stub files": {
+        "content": [
+            "hgnc_complete_set.txt",
+            "HGNC-DB-timestamp.txt",
+            [
+                "versions.yml:md5,a7b7ccbd9eda8036baf548cdf1cb6867"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-26T16:07:51.375544204"
+    }
+}
\ No newline at end of file

From 68bdf263482f41010a54c53a76887b2e3a90fee8 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 26 Nov 2024 17:45:36 +0000
Subject: [PATCH 085/887] test: add new hgnc URL and snapshots

---
 modules/local/hgnc/main.nf                 |  2 +-
 modules/local/hgnc/tests/main.nf.test      |  4 ++--
 modules/local/hgnc/tests/main.nf.test.snap | 22 ++++++++++++++++++++--
 3 files changed, 23 insertions(+), 5 deletions(-)

diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 865c59d24..a3559726c 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -18,7 +18,7 @@ process HGNC_DOWNLOAD {
 
     script:
     """
-    wget https://ftp.ebi.ac.uk/pub/databases/genenames/hgnc/tsv/hgnc_complete_set.txt
+    wget https://storage.googleapis.com/public-download-files/hgnc/tsv/tsv/hgnc_complete_set.txt
     date +%Y-%m-%d/%H:%M  > HGNC-DB-timestamp.txt
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/hgnc/tests/main.nf.test b/modules/local/hgnc/tests/main.nf.test
index 6f4c03d05..cecc4302d 100644
--- a/modules/local/hgnc/tests/main.nf.test
+++ b/modules/local/hgnc/tests/main.nf.test
@@ -18,7 +18,7 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    file(process.out.hgnc_ref[0]).name,
+                    process.out.hgnc_ref,
                     file(process.out.hgnc_date[0]).name,
                     process.out.versions
                 ).match() }
@@ -42,7 +42,7 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    file(process.out.hgnc_ref[0]).name,
+                    process.out.hgnc_ref,
                     file(process.out.hgnc_date[0]).name,
                     process.out.versions
                 ).match() }
diff --git a/modules/local/hgnc/tests/main.nf.test.snap b/modules/local/hgnc/tests/main.nf.test.snap
index 856fe2509..8512dcf6e 100644
--- a/modules/local/hgnc/tests/main.nf.test.snap
+++ b/modules/local/hgnc/tests/main.nf.test.snap
@@ -1,7 +1,25 @@
 {
     "Should create stub files": {
         "content": [
-            "hgnc_complete_set.txt",
+            [
+                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+            ],
+            "HGNC-DB-timestamp.txt",
+            [
+                "versions.yml:md5,a7b7ccbd9eda8036baf548cdf1cb6867"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-26T17:42:04.47884487"
+    },
+    "Should download HGNC files": {
+        "content": [
+            [
+                "hgnc_complete_set.txt:md5,29571d88d1648e8764b70791df6a5d2d"
+            ],
             "HGNC-DB-timestamp.txt",
             [
                 "versions.yml:md5,a7b7ccbd9eda8036baf548cdf1cb6867"
@@ -11,6 +29,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.1"
         },
-        "timestamp": "2024-11-26T16:07:51.375544204"
+        "timestamp": "2024-11-26T17:41:49.965512273"
     }
 }
\ No newline at end of file

From b7f52c61df682f30c4c53e5961dc8d4cea35d1fe Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 26 Nov 2024 17:45:55 +0000
Subject: [PATCH 086/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 40792bdb6..f619bd6bd 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,6 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Primary assembly now used as main reference genome FASTA file, as recommended by the STAR manual [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Use of only ensembl GTF file, not chr.gtf file as GTF reference file [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#508](https://github.com/nf-core/rnafusion/pull/508)
+- Add nf-test to local module: `HGNC_DOWNLOAD` [#508](https://github.com/nf-core/rnafusion/pull/509)
 
 ### Changed
 

From 0ca578b1407798db249a86303aa2855656935721 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 27 Nov 2024 15:18:13 +0000
Subject: [PATCH 087/887] refactor: remove replaced local module

---
 modules/local/rrnatranscripts/main.nf | 41 ---------------------------
 1 file changed, 41 deletions(-)
 delete mode 100644 modules/local/rrnatranscripts/main.nf

diff --git a/modules/local/rrnatranscripts/main.nf b/modules/local/rrnatranscripts/main.nf
deleted file mode 100644
index fb4ec2d62..000000000
--- a/modules/local/rrnatranscripts/main.nf
+++ /dev/null
@@ -1,41 +0,0 @@
-process RRNA_TRANSCRIPTS {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "conda-forge::python=3.12.2"
-
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/python:3.12' :
-        'biocontainers/python:3.12' }"
-
-    input:
-        tuple val(meta), path(gtf)
-
-    output:
-        tuple val(meta), path("*rrna_intervals.gtf")   , emit: rrna_gtf
-        path "versions.yml"                            , emit: versions
-
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    get_rrna_transcripts.py $gtf ${prefix}_rrna_intervals.gtf
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        python: \$(python --version | sed 's/Python //g')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}_rrna_intervals.gtf
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        python: \$(python --version | sed 's/Python //g')
-    END_VERSIONS
-    """
-}

From afea93406929d783e9377d1a23c779ad823b753b Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 27 Nov 2024 15:20:50 +0000
Subject: [PATCH 088/887] docs: update changelog

---
 CHANGELOG.md | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f619bd6bd..17518c30f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,13 +10,14 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Normalized gene expression calculated [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Primary assembly now used as main reference genome FASTA file, as recommended by the STAR manual [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Use of only ensembl GTF file, not chr.gtf file as GTF reference file [#488](https://github.com/nf-core/rnafusion/pull/488)
-- Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#508](https://github.com/nf-core/rnafusion/pull/508)
-- Add nf-test to local module: `HGNC_DOWNLOAD` [#508](https://github.com/nf-core/rnafusion/pull/509)
+- Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#539](https://github.com/nf-core/rnafusion/pull/539)
+- Add nf-test to local module: `HGNC_DOWNLOAD` [#540](https://github.com/nf-core/rnafusion/pull/540)
 
 ### Changed
 
 - Updated modules and migrated non-specific modules to nf-core/modules [#484](https://github.com/nf-core/rnafusion/pull/484)
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
+- Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 
 ### Fixed
 

From aad0c3fb6adc37cd998f0b7631f4bba25b72b829 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 27 Nov 2024 17:57:20 +0000
Subject: [PATCH 089/887] docs: update changelog

---
 CHANGELOG.md | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f619bd6bd..9b1fb2bfc 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,8 +10,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Normalized gene expression calculated [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Primary assembly now used as main reference genome FASTA file, as recommended by the STAR manual [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Use of only ensembl GTF file, not chr.gtf file as GTF reference file [#488](https://github.com/nf-core/rnafusion/pull/488)
-- Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#508](https://github.com/nf-core/rnafusion/pull/508)
-- Add nf-test to local module: `HGNC_DOWNLOAD` [#508](https://github.com/nf-core/rnafusion/pull/509)
+- Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#539](https://github.com/nf-core/rnafusion/pull/539)
+- Add nf-test to local module: `HGNC_DOWNLOAD` [#540](https://github.com/nf-core/rnafusion/pull/540)
+- Add nf-test to local subworkflow: `STRINGTIE_WORKFLOW` [#541](https://github.com/nf-core/rnafusion/pull/541)
 
 ### Changed
 

From 112ea4bfa386907ec1133572737ebe47195e0f5d Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 27 Nov 2024 17:56:40 +0000
Subject: [PATCH 090/887] test: add nf-test to local subworkflow
 `STRINGTIE_WORKFLOW`

---
 .../stringtie_workflow/tests/main.nf.test     | 70 +++++++++++++++++++
 .../tests/main.nf.test.snap                   | 56 +++++++++++++++
 2 files changed, 126 insertions(+)
 create mode 100644 subworkflows/local/stringtie_workflow/tests/main.nf.test
 create mode 100644 subworkflows/local/stringtie_workflow/tests/main.nf.test.snap

diff --git a/subworkflows/local/stringtie_workflow/tests/main.nf.test b/subworkflows/local/stringtie_workflow/tests/main.nf.test
new file mode 100644
index 000000000..da423ed57
--- /dev/null
+++ b/subworkflows/local/stringtie_workflow/tests/main.nf.test
@@ -0,0 +1,70 @@
+nextflow_workflow {
+
+    name "Test STRINGTIE_WORKFLOW"
+    script "../main.nf"
+    workflow "STRINGTIE_WORKFLOW"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "stringtie"
+
+    test("Should run stringtie workflow with stringtie = true") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                        [ id:'test', strandedness:'reverse' ], // meta map
+                    file("${params.test_data_base}/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file("${params.test_data_base}/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
+                ])
+                """
+            }
+            params {
+                stringtie = true
+                outdir = "$outputDir"
+                test_data_base = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data"
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() },
+            )
+        }
+    }
+
+    test("Should run stringtie workflow with params.all = true") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                        [ id:'test', strandedness:'reverse' ], // meta map
+                    file("${params.test_data_base}/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file("${params.test_data_base}/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
+                ])
+                """
+            }
+            params {
+                all = true
+                outdir = "$outputDir"
+                test_data_base = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data"
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() },
+            )
+        }
+    }
+
+}
diff --git a/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap b/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..791c85e41
--- /dev/null
+++ b/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
@@ -0,0 +1,56 @@
+{
+    "Should run stringtie workflow with params.all = true": {
+        "content": [
+            {
+                "0": [
+                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+                ],
+                "1": [
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
+                ],
+                "stringtie_gtf": [
+                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+                ],
+                "versions": [
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-27T17:51:10.73612885"
+    },
+    "Should run stringtie workflow with stringtie = true": {
+        "content": [
+            {
+                "0": [
+                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+                ],
+                "1": [
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
+                ],
+                "stringtie_gtf": [
+                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+                ],
+                "versions": [
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-27T17:50:57.437102535"
+    }
+}
\ No newline at end of file

From 298028e7d87b2a950dd5e4b72bfe8970d95682ce Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 27 Nov 2024 17:55:45 +0000
Subject: [PATCH 091/887] refact: move subworkflow to its own directory

---
 subworkflows/local/stringtie_workflow/main.nf | 36 +++++++++++++++++++
 workflows/rnafusion.nf                        |  2 +-
 2 files changed, 37 insertions(+), 1 deletion(-)
 create mode 100644 subworkflows/local/stringtie_workflow/main.nf

diff --git a/subworkflows/local/stringtie_workflow/main.nf b/subworkflows/local/stringtie_workflow/main.nf
new file mode 100644
index 000000000..25ff26ee8
--- /dev/null
+++ b/subworkflows/local/stringtie_workflow/main.nf
@@ -0,0 +1,36 @@
+include { STRINGTIE_STRINGTIE }    from '../../../modules/nf-core/stringtie/stringtie/main'
+include { STRINGTIE_MERGE }        from '../../../modules/nf-core/stringtie/merge/main'
+
+
+workflow STRINGTIE_WORKFLOW {
+    take:
+        bam_sorted // channel: [meta, bam]
+        ch_chrgtf  // channel: [meta, gtf]
+
+    main:
+        ch_versions = Channel.empty()
+        ch_stringtie_gtf = Channel.empty()
+
+        if ((params.stringtie || params.all) && !params.fusioninspector_only) {
+            STRINGTIE_STRINGTIE(bam_sorted, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+            ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
+
+            STRINGTIE_STRINGTIE
+                .out
+                .transcript_gtf
+                .map { it -> it[1] }
+                .set { stringtie_gtf }
+            ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
+
+
+            STRINGTIE_MERGE (stringtie_gtf, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+            ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
+            ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
+        }
+
+    emit:
+        stringtie_gtf = ch_stringtie_gtf.ifEmpty(null) // channel: [meta, gtf]
+        versions      = ch_versions                    // channel: [ path(versions.yml) ]
+
+    }
+
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index b4e4517b6..2a2c6d317 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -7,7 +7,7 @@ include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_wor
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
-include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow'
+include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
 include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
 include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'

From 320b4ca883301279fd37efc61bd5e5f2c0d6e7f9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 28 Nov 2024 16:41:11 +0100
Subject: [PATCH 092/887] add no_cosmic option/update fusionreport

---
 conf/modules.config                         | 6 ++++--
 modules/local/fusionreport/detect/main.nf   | 3 +--
 modules/local/fusionreport/download/main.nf | 3 ++-
 nextflow.config                             | 1 +
 nextflow_schema.json                        | 5 +++++
 5 files changed, 13 insertions(+), 5 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 699720400..98aeab436 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -97,7 +97,8 @@ process {
 
     withName: 'FUSIONREPORT' {
         ext.when         = { !params.skip_vis }
-        ext.args         = "--export csv"
+        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : "" }
+        ext.args2        = "--export csv"
         publishDir = [
             path: { "${params.outdir}/fusionreport/${meta.id}" },
             mode: params.publish_dir_mode,
@@ -106,7 +107,8 @@ process {
     }
 
     withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args        = { params.qiagen ? "--qiagen" : "" }
+        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : "" }
+        ext.args2        = { params.qiagen ? "--qiagen" : "" }
         publishDir = [
             path: { "${params.genomes_base}/fusion_report_db" },
             mode: params.publish_dir_mode,
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 8024d8f8e..8981e6019 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -2,8 +2,7 @@ process FUSIONREPORT {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::star=2.7.9a"
-    container "docker.io/clinicalgenomics/fusion-report:2.1.8"
+    container "docker.io/clinicalgenomics/fusion-report:3.1.0"
 
 
     input:
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 971db1b7f..6143dc052 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -3,7 +3,7 @@ process FUSIONREPORT_DOWNLOAD {
     label 'process_medium'
 
     conda "bioconda::star=2.7.9a"
-    container "docker.io/clinicalgenomics/fusion-report:2.1.8"
+    container "docker.io/clinicalgenomics/fusion-report:3.1.0"
 
     input:
     val(username)
@@ -15,6 +15,7 @@ process FUSIONREPORT_DOWNLOAD {
 
     script:
     def args = task.ext.args ?: ''
+    def args2 = task.ext.args ?: ''
     """
     fusion_report download --cosmic_usr "$username" --cosmic_passwd "$passwd" $args ./
 
diff --git a/nextflow.config b/nextflow.config
index 3ea4afd11..051c2c2fb 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -73,6 +73,7 @@ params {
     skip_vis                   = false
 
     // Path to references
+    no_cosmic                     = false
     ensembl_ref                   = "${params.genomes_base}/ensembl"
     arriba_ref                    = "${params.genomes_base}/arriba"
     arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 470b1300b..f1fa3e625 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -348,6 +348,11 @@
                     "pattern": "^\\S+\\.interval_list?$",
                     "description": "Path to ribosomal interval list.",
                     "fa_icon": "far fa-file-code"
+                },
+                "no_cosmic": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Avoid using Cosmic DB (for example in clinical case applications where a paid license applies."
                 }
             }
         },

From 23b9d3e9b28726419f1c1e31b29c0e3172ce1c9d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 28 Nov 2024 16:46:20 +0100
Subject: [PATCH 093/887] update changelog

---
 CHANGELOG.md | 9 +++++++++
 1 file changed, 9 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 17518c30f..aa3cfbb45 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -12,6 +12,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Use of only ensembl GTF file, not chr.gtf file as GTF reference file [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#539](https://github.com/nf-core/rnafusion/pull/539)
 - Add nf-test to local module: `HGNC_DOWNLOAD` [#540](https://github.com/nf-core/rnafusion/pull/540)
+- Option to avoid using COSMIC (for example in the case of clinical use) [547](https://github.com/nf-core/rnafusion/pull/547)
 
 ### Changed
 
@@ -28,6 +29,14 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 - Remove fusionGDB from documentation and fusion-report download stubs [#503](https://github.com/nf-core/rnafusion/pull/503)
 
+### Parameters
+
+
+| Old parameter                    | New parameter                     |
+| -------------------------------- | --------------------------------- |
+|                                  | `--no_cosmic`                     |
+
+
 ## v3.0.2 - [2024-04-10]
 
 ### Added

From 6514cbb1a3accd14022bbec893b299535fa11fa2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 28 Nov 2024 16:48:41 +0100
Subject: [PATCH 094/887] add option to usage documentation

---
 docs/usage.md | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/docs/usage.md b/docs/usage.md
index b7bc6794c..2a14f9fb6 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -49,6 +49,8 @@ nextflow run nf-core/rnafusion \
   --outdir <OUTPUT/PATH>
 ```
 
+If you are not covered by the research COSMIC license and want to avoid using COSMIC, you can provide the additional option `--no_cosmic`.
+
 ### Downloading the cosmic database with SANGER or QUIAGEN
 
 #### For academic users
@@ -156,6 +158,9 @@ nextflow run nf-core/rnafusion \
   --outdir <OUTPUT/PATH>
 ```
 
+If you are not covered by the research COSMIC license and want to avoid using COSMIC, you can provide the additional option `--no_cosmic`.
+
+
 > **IMPORTANT: Either `--all` or `--<tool>`** is necessary to run detection tools
 
 `--genomes_base` should be the path to the directory containing the folder `references/` that was built with `--build_references`.

From cb6671bf9e501da257eb9d0e9098026c67c955bd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 28 Nov 2024 16:49:59 +0100
Subject: [PATCH 095/887] prettier

---
 CHANGELOG.md  | 8 +++-----
 docs/usage.md | 1 -
 2 files changed, 3 insertions(+), 6 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index aa3cfbb45..663726a9f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,11 +31,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Parameters
 
-
-| Old parameter                    | New parameter                     |
-| -------------------------------- | --------------------------------- |
-|                                  | `--no_cosmic`                     |
-
+| Old parameter | New parameter |
+| ------------- | ------------- |
+|               | `--no_cosmic` |
 
 ## v3.0.2 - [2024-04-10]
 
diff --git a/docs/usage.md b/docs/usage.md
index 2a14f9fb6..b353b28ce 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -160,7 +160,6 @@ nextflow run nf-core/rnafusion \
 
 If you are not covered by the research COSMIC license and want to avoid using COSMIC, you can provide the additional option `--no_cosmic`.
 
-
 > **IMPORTANT: Either `--all` or `--<tool>`** is necessary to run detection tools
 
 `--genomes_base` should be the path to the directory containing the folder `references/` that was built with `--build_references`.

From 288c7070ed77ca2d76589eb1a3ce6c8eb805f681 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 28 Nov 2024 17:11:57 +0100
Subject: [PATCH 096/887] remove check for . in .fastq.gz fileformat

---
 assets/schema_input.json | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index 9747d48aa..a55008106 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -17,14 +17,14 @@
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "pattern": "^\\S+f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "pattern": "^\\S+f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "strandedness": {

From c739e6ce8cf44228a71243671dff9d8db587417c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 28 Nov 2024 17:16:16 +0100
Subject: [PATCH 097/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 17518c30f..aa0684578 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -18,6 +18,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated modules and migrated non-specific modules to nf-core/modules [#484](https://github.com/nf-core/rnafusion/pull/484)
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
+- Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
 
 ### Fixed
 

From eece50d96f53a8411aacc9b123ca3cbb4295845e Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Thu, 28 Nov 2024 16:35:27 +0000
Subject: [PATCH 098/887] Updated tests and fusion containers and conda
 versions, general config, and ci.yml

---
 .github/workflows/ci.yml                  |  72 ++--
 conf/igenomes.config                      | 478 ++++++++++++++++++++++
 conf/igenomes_ignored.config              |   9 +
 conf/test.config                          |   1 +
 conf/test_build.config                    |  31 ++
 conf/test_full.config                     |   4 +-
 modules/local/fusioninspector/main.nf     |   4 +-
 modules/local/starfusion/build/main.nf    |   4 +-
 modules/local/starfusion/detect/main.nf   |   4 +-
 modules/local/starfusion/download/main.nf |   4 +-
 nextflow.config                           |   3 +
 nf-test.config                            |  15 +-
 tests/.nftignore                          |   4 +
 tests/main.nf.test                        |  49 ---
 tests/nextflow.config                     |  35 --
 tests/test_build_stub.nf.test             |  72 ++++
 tests/test_build_stub.nf.test.snap        | 208 ++++++++++
 tests/test_stub.nf.test                   |  57 +++
 tests/test_stub.nf.test.snap              | 239 +++++++++++
 19 files changed, 1171 insertions(+), 122 deletions(-)
 create mode 100644 conf/igenomes.config
 create mode 100644 conf/igenomes_ignored.config
 create mode 100644 conf/test_build.config
 create mode 100644 tests/.nftignore
 delete mode 100644 tests/main.nf.test
 delete mode 100644 tests/nextflow.config
 create mode 100644 tests/test_build_stub.nf.test
 create mode 100644 tests/test_build_stub.nf.test.snap
 create mode 100644 tests/test_stub.nf.test
 create mode 100644 tests/test_stub.nf.test.snap

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index e1028ed8e..a972974dd 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -5,11 +5,18 @@ on:
     branches:
       - dev
   pull_request:
+    branches:
+      - dev
+      - master
   release:
     types: [published]
   workflow_dispatch:
 
 env:
+  NFT_DIFF: "pdiff"
+  NFT_DIFF_ARGS: "--line-numbers --expand-tabs=2"
+  NFT_VER: "0.9.2"
+  NFT_WORKDIR: "~"
   NXF_ANSI_LOG: false
   NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
   NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
@@ -20,35 +27,36 @@ concurrency:
 
 jobs:
   test:
-    name: "Run pipeline with test data (${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }})"
+    name: "${{ matrix.NXF_VER }} | ${{ matrix.test_profile }} | ${{ matrix.compute_profile }}"
     # Only run on push if this is the nf-core dev branch (merged PRs)
     if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}"
     runs-on: ubuntu-latest
     strategy:
+      fail-fast: false
       matrix:
         NXF_VER:
           - "24.04.2"
           - "latest-everything"
-        trim_parameters:
-          - "--fastp_trim false"
-          - "--fastp_trim true"
-        profile:
-          - "conda"
+        test_profile:
+          - "test"
+          - "test_scores"
+          - "test_samples"
+        compute_profile:
           - "docker"
           - "singularity"
-        test_name:
-          - "test"
         isMaster:
           - ${{ github.base_ref == 'master' }}
         # Exclude conda and singularity on dev
         exclude:
           - isMaster: false
-            profile: "conda"
+            compute_profile: "conda"
           - isMaster: false
-            profile: "singularity"
+            compute_profile: "singularity"
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+        with:
+          fetch-depth: 0
 
       - name: Set up Nextflow
         uses: nf-core/setup-nextflow@v2
@@ -56,17 +64,17 @@ jobs:
           version: "${{ matrix.NXF_VER }}"
 
       - name: Set up Apptainer
-        if: matrix.profile == 'singularity'
+        if: matrix.compute_profile == 'singularity'
         uses: eWaterCycle/setup-apptainer@main
 
       - name: Set up Singularity
-        if: matrix.profile == 'singularity'
+        if: matrix.compute_profile == 'singularity'
         run: |
           mkdir -p $NXF_SINGULARITY_CACHEDIR
           mkdir -p $NXF_SINGULARITY_LIBRARYDIR
 
       - name: Set up Miniconda
-        if: matrix.profile == 'conda'
+        if: matrix.compute_profile == 'conda'
         uses: conda-incubator/setup-miniconda@a4260408e20b96e80095f42ff7f1a15b27dd94ca # v3
         with:
           miniconda-version: "latest"
@@ -75,7 +83,7 @@ jobs:
           channels: conda-forge,bioconda
 
       - name: Set up Conda
-        if: matrix.profile == 'conda'
+        if: matrix.compute_profile == 'conda'
         run: |
           echo $(realpath $CONDA)/condabin >> $GITHUB_PATH
           echo $(realpath python) >> $GITHUB_PATH
@@ -83,16 +91,32 @@ jobs:
       - name: Clean up Disk space
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - name: "Run pipeline build with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
+      - uses: actions/setup-python@v4
+        with:
+          python-version: "3.11"
+          architecture: "x64"
+
+      - name: Install pdiff to see diff between nf-test snapshots
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} \
-          -stub --build_references \
-          --outdir /home/runner/work/rnafusion/rnafusion/results --all \
-          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references \
-          --cosmic_username ${{ secrets.COSMIC_USERNAME }} --cosmic_passwd ${{ secrets.COSMIC_PASSWD }}
+          python -m pip install --upgrade pip
+          pip install pdiff
+
+      - uses: nf-core/setup-nf-test@v1
+        with:
+          version: ${{ env.NFT_VER }}
 
-      - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
+      - name: Run Tests (${{matrix.NXF_VER}} | ${{matrix.test_profile}} | ${{matrix.compute_profile}})
         run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} -stub \
-          --outdir /home/runner/work/rnafusion/rnafusion/results --all ${{ matrix.trim_parameters }} \
-          --genomes_base /home/runner/work/rnafusion/rnafusion/results/references
+          nf-test test \
+              --ci \
+              --tag ${{matrix.test_profile}} \
+              --profile "+${{ matrix.compute_profile }}" \
+              --junitxml=test.xml \
+              --debug --verbose
+
+      - name: Publish Test Report
+        uses: mikepenz/action-junit-report@v3
+        if: always() # always run even if the previous step fails
+        with:
+          report_paths: test.xml
+          annotate_only: true
diff --git a/conf/igenomes.config b/conf/igenomes.config
new file mode 100644
index 000000000..d608b45bf
--- /dev/null
+++ b/conf/igenomes.config
@@ -0,0 +1,478 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for iGenomes paths
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines reference genomes using iGenome paths.
+    Can be used by any config that customises the base path using:
+        $params.igenomes_base / --igenomes_base
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    // illumina iGenomes reference file paths
+    genomes {
+        'GRCh37' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
+            mirtrace_species = "hsa"
+        }
+        'GRCh38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+            mirtrace_species = "hsa"
+        }
+        'CHM13' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/"
+            bwamem2     = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf"
+            gff         = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz"
+            mito_name   = "chrM"
+        }
+        'GRCm38' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
+            mirtrace_species = "mmu"
+        }
+        'TAIR10' {
+            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
+            mito_name   = "Mt"
+            mirtrace_species = "ath"
+        }
+        'EB2' {
+            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
+            // mirtrace_species = "bsu"
+        }
+        'UMD3.1' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
+            mito_name   = "MT"
+            mirtrace_species = "bta"
+        }
+        'WBcel235' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
+            mito_name   = "MtDNA"
+            macs_gsize  = "9e7"
+            mirtrace_species = "cel"
+        }
+        'CanFam3.1' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
+            mito_name   = "MT"
+            mirtrace_species = "cfa"
+        }
+        'GRCz10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            mirtrace_species = "dre"
+        }
+        'BDGP6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
+            mito_name   = "M"
+            macs_gsize  = "1.2e8"
+            mirtrace_species = "dme"
+        }
+        'EquCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
+            mito_name   = "MT"
+            // mirtrace_species = "ecb"
+        }
+        'EB1' {
+            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
+            // mirtrace_species = "ecd"
+        }
+        'Galgal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            mirtrace_species = "gga"
+        }
+        'Gm01' {
+            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
+            // mirtrace_species = "gmx"
+        }
+        'Mmul_1' {
+            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
+            mito_name   = "MT"
+            // mirtrace_species = "mcc"
+        }
+        'IRGSP-1.0' {
+            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+            mirtrace_species = "osa"
+        }
+        'CHIMP2.1.4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
+            mito_name   = "MT"
+            mirtrace_species = "ptr"
+        }
+        'Rnor_5.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            mirtrace_species = "rno"
+        }
+        'Rnor_6.0' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            mirtrace_species = "rno"
+        }
+        'R64-1-1' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
+            mito_name   = "MT"
+            macs_gsize  = "1.2e7"
+            // mirtrace_species = "sce"
+        }
+        'EF2' {
+            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
+            mito_name   = "MT"
+            macs_gsize  = "1.21e7"
+            // mirtrace_species = "spo"
+        }
+        'Sbi1' {
+            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
+            mirtrace_species = "sbi"
+        }
+        'Sscrofa10.2' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
+            mito_name   = "MT"
+            mirtrace_species = "ssc"
+        }
+        'AGPv3' {
+            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
+            mito_name   = "Mt"
+            mirtrace_species = "zma"
+        }
+        'hg38' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
+            mirtrace_species = "hsa"
+        }
+        'hg19' {
+            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "2.7e9"
+            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
+            mirtrace_species = "hsa"
+        }
+        'mm10' {
+            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.87e9"
+            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
+            mirtrace_species = "mmu"
+        }
+        'bosTau8' {
+            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            mirtrace_species = "bta"
+        }
+        'ce10' {
+            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "9e7"
+            mirtrace_species = "cel"
+        }
+        'canFam3' {
+            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
+            mito_name   = "chrM"
+            mirtrace_species = "cfa"
+        }
+        'danRer10' {
+            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.37e9"
+            mirtrace_species = "dre"
+        }
+        'dm6' {
+            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e8"
+            mirtrace_species = "dme"
+        }
+        'equCab2' {
+            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
+            mito_name   = "chrM"
+            // mirtrace_species = "ecb"
+        }
+        'galGal4' {
+            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
+            mito_name   = "chrM"
+            mirtrace_species = "gga"
+        }
+        'panTro4' {
+            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
+            mito_name   = "chrM"
+            mirtrace_species = "ptr"
+        }
+        'rn6' {
+            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
+            mito_name   = "chrM"
+            mirtrace_species = "rno"
+        }
+        'sacCer3' {
+            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
+            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
+            mito_name   = "chrM"
+            macs_gsize  = "1.2e7"
+            // mirtrace_species = "sce"
+        }
+        'susScr3' {
+            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
+            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/"
+            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
+            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
+            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
+            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
+            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
+            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
+            mito_name   = "chrM"
+            mirtrace_species = "ssc"
+        }
+    }
+}
diff --git a/conf/igenomes_ignored.config b/conf/igenomes_ignored.config
new file mode 100644
index 000000000..b4034d824
--- /dev/null
+++ b/conf/igenomes_ignored.config
@@ -0,0 +1,9 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for iGenomes paths
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Empty genomes dictionary to use when igenomes is ignored.
+----------------------------------------------------------------------------------------
+*/
+
+params.genomes = [:]
diff --git a/conf/test.config b/conf/test.config
index aa51522d7..9559e8dac 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -24,4 +24,5 @@ params {
 
     // Input data
     input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
+    all                    = true
 }
diff --git a/conf/test_build.config b/conf/test_build.config
new file mode 100644
index 000000000..138d5e308
--- /dev/null
+++ b/conf/test_build.config
@@ -0,0 +1,31 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for running minimal tests
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/rnafusion -profile test_build,<docker/singularity> --outdir <OUTDIR> -stub
+
+----------------------------------------------------------------------------------------
+*/
+
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
+params {
+    config_profile_name        = 'Test build references profile'
+    config_profile_description = 'Minimal test dataset to check pipeline function'
+
+    // Input data
+    build_references             = true
+    input                        = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
+    cosmic_username              = secrets.COSMIC_USERNAME
+    cosmic_passwd                = secrets.COSMIC_PASSWD
+    all                          = true
+}
diff --git a/conf/test_full.config b/conf/test_full.config
index eb8f5cd69..467445604 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -14,7 +14,5 @@ params {
 
     // Input data for full size test
     input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-
-    // Other params
-
+    all   = true
     }
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 2f99c7111..0f9f0abb0 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -2,8 +2,8 @@ process FUSIONINSPECTOR {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container 'docker.io/trinityctat/starfusion:1.13.0'
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
+    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index a768cc5c9..e7b969a2f 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -1,8 +1,8 @@
 process STARFUSION_BUILD {
     tag 'star-fusion'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container "docker.io/trinityctat/starfusion:1.13.0"
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
+    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 9a46cc00d..a3a433652 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -2,8 +2,8 @@ process STARFUSION {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container 'docker.io/trinityctat/starfusion:1.13.0'
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
+    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 221d12dd9..a486b607e 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,8 +1,8 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.13.0 bioconda::trinity=2.15.1 bioconda::samtools=1.19.2 bioconda::star=2.7.11b"
-    container 'docker.io/trinityctat/starfusion:1.13.0'
+    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
+    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference
diff --git a/nextflow.config b/nextflow.config
index 3ea4afd11..33f71e041 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -222,6 +222,9 @@ profiles {
     test {
         includeConfig 'conf/test.config'
     }
+    test_build {
+        includeConfig 'conf/test_build.config'
+    }
     test_full {
         includeConfig 'conf/test_full.config'
     }
diff --git a/nf-test.config b/nf-test.config
index 870799df0..cefd5a626 100644
--- a/nf-test.config
+++ b/nf-test.config
@@ -1,8 +1,17 @@
 config {
-
+    // location for all nf-tests
     testsDir "tests"
+
+    // nf-test directory including temporary files for each test
     workDir ".nf-test"
-    configFile "tests/nextflow.config"
-    profile ""
 
+    // location of library folder that is added automatically to the classpath
+    libDir "tests/pipeline/lib/"
+
+    // location of an optional nextflow.config file specific for executing tests
+    configFile "nextflow.config"
+
+    plugins {
+        load "nft-utils@0.0.3"
+    }
 }
diff --git a/tests/.nftignore b/tests/.nftignore
new file mode 100644
index 000000000..74b208734
--- /dev/null
+++ b/tests/.nftignore
@@ -0,0 +1,4 @@
+pipeline_info/*.{html,json,txt,yml}
+references
+references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
+references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
diff --git a/tests/main.nf.test b/tests/main.nf.test
deleted file mode 100644
index 715335d8f..000000000
--- a/tests/main.nf.test
+++ /dev/null
@@ -1,49 +0,0 @@
-nextflow_pipeline {
-
-    name "Test pipeline"
-    script "../main.nf"
-    tag "pipeline"
-    tag "pipeline_rnafusion"
-
-    test("Run build references with profile test") {
-
-        when {
-            params {
-                build_references             = true
-                outdir                       = "results"
-                genome_base                  = "references"
-                max_cpus                     = 2
-                max_memory                   = '6.GB'
-                max_time                     = '6.h'
-                input                        = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-                cosmic_username              = COSMIC_USERNAME
-                cosmic_passwd                = COSMIC_PASSWD
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success }
-            )
-        }
-    }
-    test("Run fusion detection with profile test") {
-
-        when {
-            params {
-                outdir                       = "results"
-                genome_base                  = "references"
-                max_cpus                     = 2
-                max_memory                   = '6.GB'
-                max_time                     = '6.h'
-                input                        = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success }
-            )
-        }
-    }
-}
diff --git a/tests/nextflow.config b/tests/nextflow.config
deleted file mode 100644
index ebd067ac8..000000000
--- a/tests/nextflow.config
+++ /dev/null
@@ -1,35 +0,0 @@
-/*
-========================================================================================
-    Nextflow config file for running tests
-========================================================================================
-*/
-
-params {
-    // Base directory for nf-core/modules test data
-    modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/'
-
-    // Base directory for nf-core/rnaseq test data
-    pipelines_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/'
-
-    outdir = 'results'
-}
-
-// Impose sensible resource limits for testing
-process {
-    withName: '.*' {
-        cpus   = 2
-        memory = 3.GB
-        time   = 2.h
-    }
-}
-
-// Impose same minimum Nextflow version as the pipeline for testing
-manifest {
-    nextflowVersion = '!>=23.04.0'
-}
-
-// Disable all Nextflow reporting options
-timeline { enabled = false }
-report   { enabled = false }
-trace    { enabled = false }
-dag      { enabled = false }
diff --git a/tests/test_build_stub.nf.test b/tests/test_build_stub.nf.test
new file mode 100644
index 000000000..76666e213
--- /dev/null
+++ b/tests/test_build_stub.nf.test
@@ -0,0 +1,72 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test_build"
+    options "-stub" // TODO remove once the pipeline is fixed
+    tag "pipeline"
+    tag "pipeline_rnafusion"
+    tag "build"
+
+    test("stub test_build with fastp_trim") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+                fastp_trim = true
+                cosmic_username = "user" // placeholder string to avoid null input val in process
+                cosmic_passwd = "password" // placeholder string to avoid null input val in process
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test_build no fastp_trim") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+                fastp_trim = false
+                cosmic_username = "user" // placeholder string to avoid null input val in process
+                cosmic_passwd = "password" // placeholder string to avoid null input val in process
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/tests/test_build_stub.nf.test.snap b/tests/test_build_stub.nf.test.snap
new file mode 100644
index 000000000..f220063ab
--- /dev/null
+++ b/tests/test_build_stub.nf.test.snap
@@ -0,0 +1,208 @@
+{
+    "stub test_build with fastp_trim": {
+        "content": [
+            14,
+            [
+                "bedops",
+                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
+                "gffread",
+                "gffread/Homo_sapiens.GRCh38.102.gff3",
+                "pipeline_info",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/ensembl",
+                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
+                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
+                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
+                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
+                "references/fusion_report_db",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v102",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ref_annot.cdna.fa",
+                "rrnatranscripts",
+                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
+            ],
+            [
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.chr.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.genepred:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.refflat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "HGNC-DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-11-28T16:11:45.948294564"
+    },
+    "stub test_build no fastp_trim": {
+        "content": [
+            14,
+            [
+                "bedops",
+                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
+                "gffread",
+                "gffread/Homo_sapiens.GRCh38.102.gff3",
+                "pipeline_info",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/ensembl",
+                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
+                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
+                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
+                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
+                "references/fusion_report_db",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v102",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ref_annot.cdna.fa",
+                "rrnatranscripts",
+                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
+            ],
+            [
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.chr.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.genepred:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102.refflat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "HGNC-DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-11-28T16:12:09.301397153"
+    }
+}
\ No newline at end of file
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
new file mode 100644
index 000000000..f2a6bf6b8
--- /dev/null
+++ b/tests/test_stub.nf.test
@@ -0,0 +1,57 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test"
+    options "-stub" // TODO remove once the pipeline is fixed
+    tag "pipeline"
+    tag "pipeline_rnafusion"
+    tag "test"
+
+    test("stub test with fastp trim") {
+
+        when {
+            params {
+                outdir     = "$outputDir"
+                fastp_trim = true
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test no fastp trim") {
+
+        when {
+            params {
+                outdir     = "$outputDir"
+                fastp_trim = false
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }
+            )
+        }
+    }
+}
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
new file mode 100644
index 000000000..9e6162460
--- /dev/null
+++ b/tests/test_stub.nf.test.snap
@@ -0,0 +1,239 @@
+{
+    "stub test with fastp trim": {
+        "content": [
+            17,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.33
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.2.0-1-g3948afb6b"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.2.0-1-g3948afb6b"
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.7.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": "2.7.10a",
+                    "samtools": 1.18,
+                    "gawk": "5.1.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": "2.7.10a",
+                    "samtools": 1.18,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.1"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
+                "fusioncatcher",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusionreport",
+                "fusionreport/test",
+                "fusionreport/test/AAA_BBB.html",
+                "fusionreport/test/test.fusionreport.tsv",
+                "fusionreport/test/test.fusionreport_filtered.tsv",
+                "fusionreport/test/test.fusions.csv",
+                "fusionreport/test/test.fusions.json",
+                "fusionreport/test/test_fusionreport_index.html",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "star_for_arriba",
+                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
+                "star_for_arriba/test.Aligned.unsort.out.bam",
+                "star_for_arriba/test.Chimeric.out.junction",
+                "star_for_arriba/test.Log.final.out",
+                "star_for_arriba/test.Log.out",
+                "star_for_arriba/test.Log.progress.out",
+                "star_for_arriba/test.ReadsPerGene.out.tab",
+                "star_for_arriba/test.SJ.out.tab",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_arriba/test.out.sam",
+                "star_for_arriba/test.sortedByCoord.out.bam",
+                "star_for_arriba/test.tab",
+                "star_for_arriba/test.toTranscriptome.out.bam",
+                "star_for_arriba/test.unmapped_1.fastq.gz",
+                "star_for_arriba/test.unmapped_2.fastq.gz",
+                "star_for_arriba/testXd.out.bam",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
+                "star_for_starfusion/test.tab",
+                "star_for_starfusion/test.toTranscriptome.out.bam",
+                "star_for_starfusion/test.unmapped_1.fastq.gz",
+                "star_for_starfusion/test.unmapped_2.fastq.gz",
+                "star_for_starfusion/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
+            ],
+            [
+                "test.arriba.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
+                "test.arriba.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
+                "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_trimmed.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_trimmed.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusioncatcher.fusion-genes.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusioncatcher.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusioncatcher.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "AAA_BBB.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusionreport.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusionreport_filtered.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusions.csv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusions.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "multiqc_report.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.bam.metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_collectinsertsize.pdf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_collectinsertsize.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_meta_info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "stringtie.merged.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-11-28T14:46:31.727687792"
+    }
+}
\ No newline at end of file

From 8a87c11386462b728f2172bee97c9f126ecbbb6c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 28 Nov 2024 16:44:48 +0000
Subject: [PATCH 099/887] test: update nf-core picard/collectrnaseqmetrics to
 include tests

---
 modules.json                                  |  2 +-
 .../collectrnaseqmetrics/environment.yml      |  2 +-
 .../picard/collectrnaseqmetrics/main.nf       |  4 +-
 .../collectrnaseqmetrics/tests/main.nf.test   | 81 +++++++++++++++++++
 .../tests/main.nf.test.snap                   | 60 ++++++++++++++
 .../tests/nextflow.config                     | 13 +++
 6 files changed, 158 insertions(+), 4 deletions(-)
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config

diff --git a/modules.json b/modules.json
index de299a717..e3709392d 100644
--- a/modules.json
+++ b/modules.json
@@ -77,7 +77,7 @@
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
                         "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
index ff4a85edb..1d715d564 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
+++ b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::picard=3.2.0
+  - bioconda::picard=3.3.0
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/main.nf b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
index 0c8f2cfa2..eb80fdc70 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/main.nf
+++ b/modules/nf-core/picard/collectrnaseqmetrics/main.nf
@@ -4,8 +4,8 @@ process PICARD_COLLECTRNASEQMETRICS {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/picard:3.2.0--hdfd78af_0' :
-        'biocontainers/picard:3.2.0--hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/picard:3.3.0--hdfd78af_0' :
+        'biocontainers/picard:3.3.0--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(bam)
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test
new file mode 100644
index 000000000..9ab185521
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test
@@ -0,0 +1,81 @@
+nextflow_process {
+
+    name "Test Process PICARD_COLLECTRNASEQMETRICS"
+    script "../main.nf"
+    process "PICARD_COLLECTRNASEQMETRICS"
+    config "./nextflow.config"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "picard"
+    tag "picard/collectrnaseqmetrics"
+    tag "ucsc/gtftogenepred"
+
+    setup {
+        run("UCSC_GTFTOGENEPRED") {
+            script "../../../ucsc/gtftogenepred/main.nf"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+    }
+
+    test("sarscov2 - fasta - gtf") {
+        
+        when {
+            process {
+                """
+                input[0] = [
+        	    [ id:'test', single_end:false, strandedness:'forward' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = UCSC_GTFTOGENEPRED.out.refflat.map{ it[1] }
+                input[2] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert file(process.out.metrics[0][1]).text.contains('CollectRnaSeqMetrics') },
+                { assert snapshot(
+                    process.out.versions,
+                    process.out.pdf
+                ).match() }
+            )
+        }
+    }
+    
+    test("sarscov2 - fasta - gtf - stub") {
+        
+        options "-stub"
+        
+        when {
+            process {
+                """
+                input[0] = [
+        	    [ id:'test', single_end:false, strandedness:'forward' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true)
+                ]
+                input[1] = UCSC_GTFTOGENEPRED.out.refflat.map{ it[1] }
+                input[2] = file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                input[3] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap
new file mode 100644
index 000000000..ad6503afd
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/tests/main.nf.test.snap
@@ -0,0 +1,60 @@
+{
+    "sarscov2 - fasta - gtf": {
+        "content": [
+            [
+                "versions.yml:md5,8d0867f89947c081711de09338138c2e"
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T16:10:39.199344417"
+    },
+    "sarscov2 - fasta - gtf - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false,
+                            "strandedness": "forward"
+                        },
+                        "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    
+                ],
+                "2": [
+                    "versions.yml:md5,8d0867f89947c081711de09338138c2e"
+                ],
+                "metrics": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false,
+                            "strandedness": "forward"
+                        },
+                        "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "pdf": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,8d0867f89947c081711de09338138c2e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-18T16:10:57.248132065"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config b/modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config
new file mode 100644
index 000000000..bc82e10c1
--- /dev/null
+++ b/modules/nf-core/picard/collectrnaseqmetrics/tests/nextflow.config
@@ -0,0 +1,13 @@
+process {
+    withName:UCSC_GTFTOGENEPRED {
+        ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons"
+    }
+    withName:PICARD_COLLECTRNASEQMETRICS {
+        ext.args = { ( meta.strandedness == "forward" || meta.single_end ) ?
+            "--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND" :
+        meta.strandedness == "reverse" ?
+        "--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND" :
+        "--STRAND_SPECIFICITY NONE"
+    }
+    }
+}

From 74195e7dc3362d964f1d4103fd2945807db66083 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 28 Nov 2024 16:47:56 +0000
Subject: [PATCH 100/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 17518c30f..c56866b39 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -12,6 +12,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Use of only ensembl GTF file, not chr.gtf file as GTF reference file [#488](https://github.com/nf-core/rnafusion/pull/488)
 - Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#539](https://github.com/nf-core/rnafusion/pull/539)
 - Add nf-test to local module: `HGNC_DOWNLOAD` [#540](https://github.com/nf-core/rnafusion/pull/540)
+- Add nf-test to nf-core module: `PICARD_COLLECTRNASEQMETRICS` and update module [#551](https://github.com/nf-core/rnafusion/pull/551)
 
 ### Changed
 

From 704d23dbec94a765b820b7a07019dc1e93e95695 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Thu, 28 Nov 2024 21:07:25 +0000
Subject: [PATCH 101/887] Updated ci.yml and nftests

---
 .github/workflows/ci.yml           |   6 +-
 conf/test.config                   |   1 -
 conf/test_build.config             |   3 -
 conf/test_cosmic.config            |  30 ++++
 nextflow.config                    |   5 +-
 tests/.nftignore                   |   1 -
 tests/test.nf.test                 |  71 +++++++++
 tests/test_build.nf.test           |  71 +++++++++
 tests/test_build_stub.nf.test      |  16 +-
 tests/test_build_stub.nf.test.snap |   4 +-
 tests/test_cosmic.nf.test          | 102 ++++++++++++
 tests/test_stub.nf.test            |  33 +++-
 tests/test_stub.nf.test.snap       | 241 ++++++++++++++++++++++++++++-
 13 files changed, 558 insertions(+), 26 deletions(-)
 create mode 100644 conf/test_cosmic.config
 create mode 100644 tests/test.nf.test
 create mode 100644 tests/test_build.nf.test
 create mode 100644 tests/test_cosmic.nf.test

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index a972974dd..2e06ffabc 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -38,12 +38,12 @@ jobs:
           - "24.04.2"
           - "latest-everything"
         test_profile:
-          - "test"
-          - "test_scores"
-          - "test_samples"
+          - "test_stub"
+          - "test_build_stub"
         compute_profile:
           - "docker"
           - "singularity"
+          - "conda"
         isMaster:
           - ${{ github.base_ref == 'master' }}
         # Exclude conda and singularity on dev
diff --git a/conf/test.config b/conf/test.config
index 9559e8dac..aa51522d7 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -24,5 +24,4 @@ params {
 
     // Input data
     input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-    all                    = true
 }
diff --git a/conf/test_build.config b/conf/test_build.config
index 138d5e308..32ee1bb62 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -25,7 +25,4 @@ params {
     // Input data
     build_references             = true
     input                        = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-    cosmic_username              = secrets.COSMIC_USERNAME
-    cosmic_passwd                = secrets.COSMIC_PASSWD
-    all                          = true
 }
diff --git a/conf/test_cosmic.config b/conf/test_cosmic.config
new file mode 100644
index 000000000..ebbad7cf3
--- /dev/null
+++ b/conf/test_cosmic.config
@@ -0,0 +1,30 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for running minimal tests
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines input files and everything required to run a fast and simple pipeline test.
+
+    Use as follows:
+        nextflow run nf-core/rnafusion -profile test_cosmic,<docker/singularity> --outdir <OUTDIR> -stub
+
+----------------------------------------------------------------------------------------
+*/
+
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
+
+params {
+    config_profile_name        = 'Test cosmic profile'
+    config_profile_description = 'Minimal test cosmic dataset to check pipeline function'
+
+    // Input data
+    input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
+    all                    = true
+    cosmic_username        = secrets.COSMIC_USERNAME
+    cosmic_passwd          = secrets.COSMIC_PASSWD
+}
diff --git a/nextflow.config b/nextflow.config
index 33f71e041..0f0a00a7b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -225,7 +225,10 @@ profiles {
     test_build {
         includeConfig 'conf/test_build.config'
     }
-    test_full {
+    test_cosmic {
+        includeConfig 'conf/test_cosmic.config'
+    }
+        test_full {
         includeConfig 'conf/test_full.config'
     }
 
diff --git a/tests/.nftignore b/tests/.nftignore
index 74b208734..d70cc56e9 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -1,4 +1,3 @@
 pipeline_info/*.{html,json,txt,yml}
-references
 references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
 references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
diff --git a/tests/test.nf.test b/tests/test.nf.test
new file mode 100644
index 000000000..f56807286
--- /dev/null
+++ b/tests/test.nf.test
@@ -0,0 +1,71 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test"
+    tag "pipeline"
+    tag "pipeline_rnafusion"
+    tag "test"
+
+    test("test with fastp trim") {
+
+        when {
+            params {
+                outdir               = "$outputDir"
+                fastp_trim           = true
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+
+    test("test no fastp trim") {
+
+        when {
+            params {
+                outdir     = "$outputDir"
+                fastp_trim = false
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
new file mode 100644
index 000000000..ef18e7e1d
--- /dev/null
+++ b/tests/test_build.nf.test
@@ -0,0 +1,71 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test_build"
+    tag "pipeline"
+    tag "pipeline_rnafusion"
+    tag "test_build"
+
+    test("test_build with fastp_trim") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+                fastp_trim = true
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+
+    test("test_build no fastp_trim") {
+
+        when {
+            params {
+                outdir     = "$outputDir"
+                fastp_trim = false
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/tests/test_build_stub.nf.test b/tests/test_build_stub.nf.test
index 76666e213..cfb703512 100644
--- a/tests/test_build_stub.nf.test
+++ b/tests/test_build_stub.nf.test
@@ -6,16 +6,17 @@ nextflow_pipeline {
     options "-stub" // TODO remove once the pipeline is fixed
     tag "pipeline"
     tag "pipeline_rnafusion"
-    tag "build"
+    tag "test_build_stub"
 
     test("stub test_build with fastp_trim") {
 
         when {
             params {
-                outdir = "$outputDir"
-                fastp_trim = true
+                outdir          = "$outputDir"
+                fastp_trim      = true
                 cosmic_username = "user" // placeholder string to avoid null input val in process
-                cosmic_passwd = "password" // placeholder string to avoid null input val in process
+                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
+                all             = true
             }
         }
 
@@ -43,10 +44,11 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir = "$outputDir"
-                fastp_trim = false
+                outdir          = "$outputDir"
+                fastp_trim      = true
                 cosmic_username = "user" // placeholder string to avoid null input val in process
-                cosmic_passwd = "password" // placeholder string to avoid null input val in process
+                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
+                all             = true
             }
         }
 
diff --git a/tests/test_build_stub.nf.test.snap b/tests/test_build_stub.nf.test.snap
index f220063ab..a9bc48ee2 100644
--- a/tests/test_build_stub.nf.test.snap
+++ b/tests/test_build_stub.nf.test.snap
@@ -100,7 +100,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-28T16:11:45.948294564"
+        "timestamp": "2024-11-28T21:05:36.346682866"
     },
     "stub test_build no fastp_trim": {
         "content": [
@@ -203,6 +203,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-28T16:12:09.301397153"
+        "timestamp": "2024-11-28T21:06:01.432893263"
     }
 }
\ No newline at end of file
diff --git a/tests/test_cosmic.nf.test b/tests/test_cosmic.nf.test
new file mode 100644
index 000000000..ffa1e4896
--- /dev/null
+++ b/tests/test_cosmic.nf.test
@@ -0,0 +1,102 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test_cosmic"
+    tag "pipeline"
+    tag "pipeline_rnafusion"
+    tag "test_cosmic"
+
+    test("test cosmic with fastp trim") {
+
+        when {
+            params {
+                outdir               = "$outputDir"
+                fastp_trim           = true
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+
+    test("test cosmic no fastp trim") {
+
+        when {
+            params {
+                outdir     = "$outputDir"
+                fastp_trim = false
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+
+    test("test cosmic no fastp trim build") {
+
+        when {
+            params {
+                outdir     = "$outputDir"
+                fastp_trim = false
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index f2a6bf6b8..b2ad98ce6 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -6,14 +6,17 @@ nextflow_pipeline {
     options "-stub" // TODO remove once the pipeline is fixed
     tag "pipeline"
     tag "pipeline_rnafusion"
-    tag "test"
+    tag "test_stub"
 
     test("stub test with fastp trim") {
 
         when {
             params {
-                outdir     = "$outputDir"
-                fastp_trim = true
+                outdir          = "$outputDir"
+                fastp_trim      = true
+                cosmic_username = "user" // placeholder string to avoid null input val in process
+                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
+                all             = true
             }
         }
 
@@ -43,14 +46,32 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir     = "$outputDir"
-                fastp_trim = false
+                outdir          = "$outputDir"
+                fastp_trim      = true
+                cosmic_username = "user" // placeholder string to avoid null input val in process
+                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
+                all             = true
             }
         }
 
         then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
             assertAll(
-                { assert workflow.success }
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
             )
         }
     }
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 9e6162460..c09b29194 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "stub test with fastp trim": {
+    "stub test no fastp trim": {
         "content": [
             17,
             {
@@ -29,8 +29,245 @@
                     "picard": "3.2.0-1-g3948afb6b"
                 },
                 "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.7.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": "2.7.10a",
+                    "samtools": 1.18,
+                    "gawk": "5.1.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": "2.7.10a",
+                    "samtools": 1.18,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.1"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
+                "fusioncatcher",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusionreport",
+                "fusionreport/test",
+                "fusionreport/test/AAA_BBB.html",
+                "fusionreport/test/test.fusionreport.tsv",
+                "fusionreport/test/test.fusionreport_filtered.tsv",
+                "fusionreport/test/test.fusions.csv",
+                "fusionreport/test/test.fusions.json",
+                "fusionreport/test/test_fusionreport_index.html",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "star_for_arriba",
+                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
+                "star_for_arriba/test.Aligned.unsort.out.bam",
+                "star_for_arriba/test.Chimeric.out.junction",
+                "star_for_arriba/test.Log.final.out",
+                "star_for_arriba/test.Log.out",
+                "star_for_arriba/test.Log.progress.out",
+                "star_for_arriba/test.ReadsPerGene.out.tab",
+                "star_for_arriba/test.SJ.out.tab",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_arriba/test.out.sam",
+                "star_for_arriba/test.sortedByCoord.out.bam",
+                "star_for_arriba/test.tab",
+                "star_for_arriba/test.toTranscriptome.out.bam",
+                "star_for_arriba/test.unmapped_1.fastq.gz",
+                "star_for_arriba/test.unmapped_2.fastq.gz",
+                "star_for_arriba/testXd.out.bam",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
+                "star_for_starfusion/test.tab",
+                "star_for_starfusion/test.toTranscriptome.out.bam",
+                "star_for_starfusion/test.unmapped_1.fastq.gz",
+                "star_for_starfusion/test.unmapped_2.fastq.gz",
+                "star_for_starfusion/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
+            ],
+            [
+                "test.arriba.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
+                "test.arriba.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
+                "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_trimmed.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_trimmed.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusioncatcher.fusion-genes.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusioncatcher.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusioncatcher.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "AAA_BBB.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusionreport.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusionreport_filtered.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusions.csv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusions.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "multiqc_report.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.bam.metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_collectinsertsize.pdf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_collectinsertsize.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_meta_info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "stringtie.merged.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-11-28T21:06:57.215801319"
+    },
+    "stub test with fastp trim": {
+        "content": [
+            17,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.33
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.2.0-1-g3948afb6b"
                 },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
                 "SAMTOOLS_INDEX_FOR_STARFUSION": {
                     "samtools": 1.21
                 },
@@ -234,6 +471,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-28T14:46:31.727687792"
+        "timestamp": "2024-11-28T21:06:29.861338628"
     }
 }
\ No newline at end of file

From fa4f7713ac3bacbc57c7686d6770e8dbdb10a88e Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Thu, 28 Nov 2024 21:28:34 +0000
Subject: [PATCH 102/887] Update tests

---
 tests/test.nf.test            |  6 ++++--
 tests/test_build.nf.test      |  2 ++
 tests/test_build_stub.nf.test |  2 +-
 tests/test_stub.nf.test       |  2 +-
 tests/test_stub.nf.test.snap  | 26 ++------------------------
 5 files changed, 10 insertions(+), 28 deletions(-)

diff --git a/tests/test.nf.test b/tests/test.nf.test
index f56807286..be6ab52c2 100644
--- a/tests/test.nf.test
+++ b/tests/test.nf.test
@@ -11,8 +11,9 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir               = "$outputDir"
-                fastp_trim           = true
+                outdir     = "$outputDir"
+                fastp_trim = true
+                no_cosmic  = true
             }
         }
 
@@ -44,6 +45,7 @@ nextflow_pipeline {
             params {
                 outdir     = "$outputDir"
                 fastp_trim = false
+                no_cosmic  = true
             }
         }
 
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index ef18e7e1d..a6a9936ac 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -13,6 +13,7 @@ nextflow_pipeline {
             params {
                 outdir = "$outputDir"
                 fastp_trim = true
+                no_cosmic  = true
             }
         }
 
@@ -44,6 +45,7 @@ nextflow_pipeline {
             params {
                 outdir     = "$outputDir"
                 fastp_trim = false
+                no_cosmic  = true
             }
         }
 
diff --git a/tests/test_build_stub.nf.test b/tests/test_build_stub.nf.test
index cfb703512..6844867d6 100644
--- a/tests/test_build_stub.nf.test
+++ b/tests/test_build_stub.nf.test
@@ -45,7 +45,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir          = "$outputDir"
-                fastp_trim      = true
+                fastp_trim      = false
                 cosmic_username = "user" // placeholder string to avoid null input val in process
                 cosmic_passwd   = "password" // placeholder string to avoid null input val in process
                 all             = true
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index b2ad98ce6..90d9bb647 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -47,7 +47,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir          = "$outputDir"
-                fastp_trim      = true
+                fastp_trim      = false
                 cosmic_username = "user" // placeholder string to avoid null input val in process
                 cosmic_passwd   = "password" // placeholder string to avoid null input val in process
                 all             = true
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index c09b29194..63dc16109 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,20 +1,14 @@
 {
     "stub test no fastp trim": {
         "content": [
-            17,
+            15,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
-                "FASTP": {
-                    "fastp": "0.23.4"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FASTQC_FOR_FASTP": {
-                    "fastqc": "0.12.1"
-                },
                 "FUSIONCATCHER": {
                     "fusioncatcher": 1.33
                 },
@@ -61,18 +55,9 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "fastp",
-                "fastp/test.fastp.html",
-                "fastp/test.fastp.json",
-                "fastp/test.fastp.log",
-                "fastp/test_1.fastp.fastq.gz",
-                "fastp/test_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
                 "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
                 "fusioncatcher/test.fusioncatcher.log",
@@ -156,15 +141,8 @@
             [
                 "test.arriba.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
                 "test.arriba.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
-                "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                 "test.html:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_trimmed.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_trimmed.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test.fusioncatcher.fusion-genes.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test.fusioncatcher.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test.fusioncatcher.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -234,7 +212,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-28T21:06:57.215801319"
+        "timestamp": "2024-11-28T21:25:48.332933141"
     },
     "stub test with fastp trim": {
         "content": [

From 460b6450ced2459dc4f2ce8abc5368dc5bc872c3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 29 Nov 2024 09:18:26 +0100
Subject: [PATCH 103/887] add parameter interpretation in modules.config

---
 conf/modules.config                           |  18 +-
 main.nf                                       |   6 +-
 modules/local/fusionreport/detect/main.nf     |   6 +-
 modules/local/fusionreport/download/main.nf   |   2 +-
 .../utils_nfcore_rnafusion_pipeline/main.nf   |   3 +-
 workflows/rnafusion.nf                        | 182 +++++++++---------
 6 files changed, 104 insertions(+), 113 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index b07f53335..3b176c87e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -36,7 +36,7 @@ process {
     }
 
     withName: 'ARRIBA_VISUALISATION' {
-        ext.when   = { !params.fusioninspector_only && (params.starfusion || params.all) }
+        ext.when   = { {!params.fusioninspector_only} && ({params.starfusion} || {params.all}) }
         ext.prefix = { "${meta.id}_combined_fusions_arriba_visualisation" }
         publishDir = [
             path: { "${params.outdir}/arriba_visualisation" },
@@ -63,7 +63,7 @@ process {
 
     withName: 'FASTQC' {
         ext.args = '--quiet'
-        ext.when = { !params.skip_qc && !params.references_only }
+        ext.when = { {!params.skip_qc} && {!params.references_only} }
         publishDir = [
             path: { "${params.outdir}/fastqc" },
             mode: params.publish_dir_mode,
@@ -73,7 +73,7 @@ process {
 
     withName: 'FASTQC_FOR_FASTP' {
         ext.args = '--quiet'
-        ext.when = ( !params.skip_qc)
+        ext.when = { !params.skip_qc }
         ext.prefix = { "${meta.id}_trimmed" }
         publishDir = [
             path: { "${params.outdir}/fastqc_for_fastp" },
@@ -96,7 +96,7 @@ process {
 
     withName: 'FUSIONINSPECTOR' {
         ext.when = { !params.skip_vis }
-        ext.args = { params.fusioninspector_limitSjdbInsertNsj != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
+        ext.args = { ${params.fusioninspector_limitSjdbInsertNsj} != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
         ext.args2 = '--annotate --examine_coding_effect'
     }
 
@@ -111,7 +111,7 @@ process {
     }
 
     withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args        = { params.qiagen ? "--qiagen" : "" }
+        ext.args        = { ${params.qiagen} ? "--qiagen" : "" }
         publishDir = [
             path: { "${params.genomes_base}/fusion_report_db" },
             mode: params.publish_dir_mode,
@@ -153,7 +153,7 @@ process {
     }
     withName: 'MULTIQC' {
         ext.when         = { !params.skip_qc }
-        ext.args   = params.multiqc_title ? "--title \"$params.multiqc_title\"" : ''
+        ext.args   = {params.multiqc_title} ? "--title \"$params.multiqc_title\"" : ''
         publishDir = [
             path: { "${params.outdir}/multiqc" },
             mode: params.publish_dir_mode,
@@ -162,12 +162,12 @@ process {
     }
 
     withName: 'PICARD_COLLECTRNASEQMETRICS' {
-        ext.when         = { !params.skip_qc && !params.fusioninspector_only && (params.starfusion || params.all) }
+        ext.when         = { {!params.skip_qc} && {!params.fusioninspector_only} && ( {params.starfusion} || {params.all}) }
 
     }
 
     withName: 'GATK4_MARKDUPLICATES' {
-        ext.when         = { !params.skip_qc && !params.fusioninspector_only && (params.starfusion || params.all) }
+        ext.when         = { {!params.skip_qc} && {!params.fusioninspector_only} && ( {params.starfusion}|| {params.all}) }
         publishDir = [
             path: { "${params.outdir}/picard" },
             mode: params.publish_dir_mode,
@@ -176,7 +176,7 @@ process {
     }
 
     withName: 'PICARD_COLLECTINSERTSIZEMETRICS' {
-        ext.when         = { !params.skip_qc && !params.fusioninspector_only && (params.starfusion || params.all) }
+        ext.when         = { ${!params.skip_qc} && ${!params.fusioninspector_only} && (${params.starfusion} || ${params.all}) }
         ext.prefix       = { "${meta.id}_collectinsertsize"}
         publishDir = [
             path: { "${params.outdir}/picard" },
diff --git a/main.nf b/main.nf
index 09216f56b..78781bed3 100644
--- a/main.nf
+++ b/main.nf
@@ -51,6 +51,9 @@ workflow NFCORE_RNAFUSION {
     //
 
     RNAFUSION(samplesheet)
+
+    emit:
+    multiqc_report = RNAFUSION.out.multiqc_report
 }
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -67,10 +70,8 @@ workflow {
     PIPELINE_INITIALISATION (
         params.version,
         params.validate_params,
-        params.monochrome_logs,
         args,
         params.outdir,
-        params.input
     )
 
     //
@@ -88,6 +89,7 @@ workflow {
         params.outdir,
         params.monochrome_logs,
         params.hook_url,
+        NFCORE_RNAFUSION.out.multiqc_report,
     )
 }
 
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index f2557bf01..ec7a076c8 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -58,11 +58,7 @@ process FUSIONREPORT {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusion_report: sth
+        fusion_report: \$(fusion_report --version | sed 's/fusion-report //')
     END_VERSIONS
     """
 }
-    // cat <<-END_VERSIONS > versions.yml
-    // "${task.process}":
-    //     fusion_report: \$(fusion_report --version 2>&1 | sed 's/fusion-report //')
-    // END_VERSIONS
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 971db1b7f..04e069ee0 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -10,7 +10,7 @@ process FUSIONREPORT_DOWNLOAD {
     val(passwd)
 
     output:
-    path "*"                , emit: reference
+    path "*.db"             , emit: reference
     path "versions.yml"     , emit: versions
 
     script:
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index ae62c8d4c..b217a88d8 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -30,10 +30,8 @@ workflow PIPELINE_INITIALISATION {
     take:
     version           // boolean: Display version and exit
     validate_params   // boolean: Boolean whether to validate parameters against the schema at runtime
-    monochrome_logs   // boolean: Do not use coloured log outputs
     nextflow_cli_args //   array: List of positional nextflow CLI args
     outdir            //  string: The output directory where the results will be saved
-    input             //  string: Path to input samplesheet
 
     main:
 
@@ -114,6 +112,7 @@ workflow PIPELINE_COMPLETION {
     outdir          //    path: Path to output directory where results will be published
     monochrome_logs // boolean: Disable ANSI colour codes in log output
     hook_url        //  string: hook URL for notifications
+    multiqc_report  //  string: Path to MultiQC report
 
     main:
     summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 238bcc3af..e14ac799c 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -13,6 +13,10 @@ include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringti
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
 include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
 include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
+include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
+include { paramsSummaryMap              }   from 'plugin/nf-schema'
+include { paramsSummaryMultiqc          }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
+include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 
 include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
@@ -63,9 +67,10 @@ workflow RNAFUSION {
     ch_reads = TRIM_WORKFLOW.out.trimmed_reads
     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
-    // SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ meta, index ->  index  }, BUILD_REFERENCES.out.ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')
-    // ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.zi.collect{it[1]})
-    // ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
+
+    SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
+    ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
+    ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
 
 
     //
@@ -139,97 +144,86 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
 
 
-    // //QC
-    // QC_WORKFLOW (
-    //     ch_reads,
-    //     STARFUSION_WORKFLOW.out.ch_bam_sorted,
-    //     STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-    //     BUILD_REFERENCES.out.ch_gtf,
-    //     BUILD_REFERENCES.out.ch_refflat,
-    //     BUILD_REFERENCES.out.ch_fasta,
-    //     BUILD_REFERENCES.out.ch_fai,
-    //     BUILD_REFERENCES.out.ch_rrna_interval
-    // )
-    // ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
-
-//     //
-//     // Collate and save software versions
-//     //
-//     softwareVersionsToYAML(ch_versions)
-//         .collectFile(
-//             storeDir: "${params.outdir}/pipeline_info",
-//             name: 'nf_core_pipeline_software_mqc_versions.yml',
-//             sort: true,
-//             newLine: true
-//         ).set { ch_collated_versions }
-
-
-    // //
-    // // Collate and save software versions
-    // //
-    // softwareVersionsToYAML(ch_versions)
-    //     .collectFile(
-    //         storeDir: "${params.outdir}/pipeline_info",
-    //         name: 'nf_core_'  + 'pipeline_software_' +  'mqc_'  + 'versions.yml',
-    //         sort: true,
-    //         newLine: true
-    //     ).set { ch_collated_versions }
-
-    // //
-    // // MODULE: MultiQC
-    // //
-    // ch_multiqc_config        = Channel.fromPath(
-    //     "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-    // ch_multiqc_custom_config = params.multiqc_config ?
-    //     Channel.fromPath(params.multiqc_config, checkIfExists: true) :
-    //     Channel.empty()
-    // ch_multiqc_logo          = params.multiqc_logo ?
-    //     Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
-    //     Channel.empty()
-
-    // summary_params      = paramsSummaryMap(
-    //     workflow, parameters_schema: "nextflow_schema.json")
-    // ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
-    // ch_multiqc_files = ch_multiqc_files.mix(
-    //     ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    // ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
-    //     file(params.multiqc_methods_description, checkIfExists: true) :
-    //     file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    // ch_methods_description                = Channel.value(
-    //     methodsDescriptionText(ch_multiqc_custom_methods_description))
-
-    // ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
-    // ch_multiqc_files = ch_multiqc_files.mix(
-    //     ch_methods_description.collectFile(
-    //         name: 'methods_description_mqc.yaml',
-    //         sort: true
-    //     )
-    // )
-    // ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
-    // ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
-
-    // MULTIQC (
-    //     ch_multiqc_files.collect(),
-    //     ch_multiqc_config.toList(),
-    //     ch_multiqc_custom_config.toList(),
-    //     ch_multiqc_logo.toList(),
-    //     [],
-    //     []
-    // )
-
-
-
-    // emit:
-    // multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
-    // versions       = ch_versions                 // channel: [ path(versions.yml) ]
+    //QC
+    QC_WORKFLOW (
+        ch_reads,
+        STARFUSION_WORKFLOW.out.ch_bam_sorted,
+        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+        BUILD_REFERENCES.out.ch_gtf,
+        BUILD_REFERENCES.out.ch_refflat,
+        BUILD_REFERENCES.out.ch_fasta,
+        BUILD_REFERENCES.out.ch_fai,
+        BUILD_REFERENCES.out.ch_rrna_interval
+    )
+    ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
+
+    //
+    // Collate and save software versions
+    //
+    softwareVersionsToYAML(ch_versions)
+        .collectFile(
+            storeDir: "${params.outdir}/pipeline_info",
+            name: 'nf_core_pipeline_software_mqc_versions.yml',
+            sort: true,
+            newLine: true
+        ).set { ch_collated_versions }
+
+
+    //
+    // MODULE: MultiQC
+    //
+    ch_multiqc_config        = Channel.fromPath(
+        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+    ch_multiqc_custom_config = params.multiqc_config ?
+        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+        Channel.empty()
+    ch_multiqc_logo          = params.multiqc_logo ?
+        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+        Channel.empty()
+
+    summary_params      = paramsSummaryMap(
+        workflow, parameters_schema: "nextflow_schema.json")
+    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
+        file(params.multiqc_methods_description, checkIfExists: true) :
+        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+    ch_methods_description                = Channel.value(
+        methodsDescriptionText(ch_multiqc_custom_methods_description))
+
+    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
+    ch_multiqc_files = ch_multiqc_files.mix(
+        ch_methods_description.collectFile(
+            name: 'methods_description_mqc.yaml',
+            sort: true
+        )
+    )
+    ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.fastp_html.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.fastp_json.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.fastqc_trimmed.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
+    ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
+
+    MULTIQC (
+        ch_multiqc_files.collect(),
+        ch_multiqc_config.toList(),
+        ch_multiqc_custom_config.toList(),
+        ch_multiqc_logo.toList(),
+        [],
+        []
+    )
+
+
+
+    emit:
+    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
+    versions       = ch_versions                 // channel: [ path(versions.yml) ]
 
 }
 

From 098a92dcb85e5a492adc3eab62074b617d569177 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 29 Nov 2024 10:47:23 +0100
Subject: [PATCH 104/887] add skip_vcf param

---
 conf/modules.config  | 2 +-
 nextflow.config      | 1 +
 nextflow_schema.json | 4 ++++
 3 files changed, 6 insertions(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 699720400..eb1f554b5 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -354,6 +354,6 @@ process {
     }
 
     withName: 'VCF_COLLECT' {
-        ext.when = {!params.fusioninspector_only}
+        ext.when = { {!params.fusioninspector_only} && {!params.skip_vcf} }
     }
 }
diff --git a/nextflow.config b/nextflow.config
index 3ea4afd11..bddd64b0c 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -71,6 +71,7 @@ params {
     // Skip steps
     skip_qc                    = false
     skip_vis                   = false
+    skip_vcf                   = false
 
     // Path to references
     ensembl_ref                   = "${params.genomes_base}/ensembl"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 470b1300b..9899ea151 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -18,6 +18,10 @@
                 "skip_vis": {
                     "type": "boolean",
                     "description": "Skip visualisation steps"
+                },
+                "skip_vcf": {
+                    "type": "boolean",
+                    "description": "Skip vcf generation step"
                 }
             },
             "fa_icon": "fas fa-fast-forward"

From 7e1fa66080d26cbdd0e8b37aff9e775ebc5eed11 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 29 Nov 2024 11:12:22 +0100
Subject: [PATCH 105/887] changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 70b049baa..79a5db4ce 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,6 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `ENSEMBL_DOWNLOAD` [#539](https://github.com/nf-core/rnafusion/pull/539)
 - Add nf-test to local module: `HGNC_DOWNLOAD` [#540](https://github.com/nf-core/rnafusion/pull/540)
 - Add nf-test to nf-core module: `PICARD_COLLECTRNASEQMETRICS` and update module [#551](https://github.com/nf-core/rnafusion/pull/551)
+- Add `--skip_vcf` boolean parameter to skip vcf file generation [#554](https://github.com/nf-core/rnafusion/pull/554)
 
 ### Changed
 

From 345a562e0326650bb618a66379a84ee001b73a95 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 29 Nov 2024 16:10:49 +0000
Subject: [PATCH 106/887] fix: fix bug in `fusionreport_download` when building
 references

---
 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 5 +++++
 workflows/build_references.nf                              | 2 +-
 2 files changed, 6 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index ae62c8d4c..258c60500 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -156,6 +156,11 @@ workflow PIPELINE_COMPLETION {
 //
 def validateInputParameters() {
     genomeExistsError()
+
+    if (params.build_references && params.no_cosmic) {
+            log.warn("No cosmic credentials were provided. Skipping `FUSIONREPORT_DOWNLOAD`")
+    }
+
 }
 
 //
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index da6664373..8abce2f49 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -76,7 +76,7 @@ workflow BUILD_REFERENCES {
     } else {
         UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.chrgtf)
     }
-    if (params.fusionreport || params.all) {
+    if ((params.fusionreport || params.all) && !params.no_cosmic) {
         FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
     }
 

From b3d0f94eee62d22ed6d8c088ea991f3578c4f1f3 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 29 Nov 2024 16:10:57 +0000
Subject: [PATCH 107/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8eb7d8caf..392f4ba5f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 - Fixed some Nextflow run-commands in the docs [#491](https://github.com/nf-core/rnafusion/pull/491)
 - Fixed bug when trying to build indices behind a proxy and wget was unable to download arriba indices [#495](https://github.com/nf-core/rnafusion/issues/495)
+- Fixed bug in `FUSIONREPORT_DOWNLOAD` when building references with `--no_cosmic parameter` [#555](https://github.com/nf-core/rnafusion/issues/555)
 
 ### Removed
 

From 88deea5d83c04644f0904282bd095f1a632b3c9c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 29 Nov 2024 16:17:42 +0000
Subject: [PATCH 108/887] ci: add `--no_cosmic` flag for tests in CI

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index e1028ed8e..2751a4760 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -89,7 +89,7 @@ jobs:
           -stub --build_references \
           --outdir /home/runner/work/rnafusion/rnafusion/results --all \
           --genomes_base /home/runner/work/rnafusion/rnafusion/results/references \
-          --cosmic_username ${{ secrets.COSMIC_USERNAME }} --cosmic_passwd ${{ secrets.COSMIC_PASSWD }}
+          --no_cosmic
 
       - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
         run: |

From 90d309d0fadae86c9b9a177790e723a941bc212f Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Fri, 29 Nov 2024 16:36:06 +0000
Subject: [PATCH 109/887] Update test_build test and add it to ci

---
 .github/workflows/ci.yml      |  1 +
 tests/test_build.nf.test      | 39 +----------------------------------
 tests/test_build_stub.nf.test | 35 +------------------------------
 3 files changed, 3 insertions(+), 72 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 2e06ffabc..2cc8fba44 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -40,6 +40,7 @@ jobs:
         test_profile:
           - "test_stub"
           - "test_build_stub"
+          - "test_build"
         compute_profile:
           - "docker"
           - "singularity"
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index a6a9936ac..a800b0e6b 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -7,12 +7,11 @@ nextflow_pipeline {
     tag "pipeline_rnafusion"
     tag "test_build"
 
-    test("test_build with fastp_trim") {
+    test("test_build") {
 
         when {
             params {
                 outdir = "$outputDir"
-                fastp_trim = true
                 no_cosmic  = true
             }
         }
@@ -26,42 +25,6 @@ nextflow_pipeline {
             assertAll(
                 { assert workflow.success},
                 { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-
-    test("test_build no fastp_trim") {
-
-        when {
-            params {
-                outdir     = "$outputDir"
-                fastp_trim = false
-                no_cosmic  = true
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
diff --git a/tests/test_build_stub.nf.test b/tests/test_build_stub.nf.test
index 6844867d6..3235720ac 100644
--- a/tests/test_build_stub.nf.test
+++ b/tests/test_build_stub.nf.test
@@ -8,44 +8,11 @@ nextflow_pipeline {
     tag "pipeline_rnafusion"
     tag "test_build_stub"
 
-    test("stub test_build with fastp_trim") {
+    test("stub test_build") {
 
         when {
             params {
                 outdir          = "$outputDir"
-                fastp_trim      = true
-                cosmic_username = "user" // placeholder string to avoid null input val in process
-                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
-                all             = true
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-
-    test("stub test_build no fastp_trim") {
-
-        when {
-            params {
-                outdir          = "$outputDir"
-                fastp_trim      = false
                 cosmic_username = "user" // placeholder string to avoid null input val in process
                 cosmic_passwd   = "password" // placeholder string to avoid null input val in process
                 all             = true

From 18bf7c0e447476b797ba2ab0cf01bf649dbc5c20 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Fri, 29 Nov 2024 17:50:59 +0000
Subject: [PATCH 110/887] Update test_build_stub

---
 tests/test_build_stub.nf.test.snap | 107 +----------------------------
 1 file changed, 2 insertions(+), 105 deletions(-)

diff --git a/tests/test_build_stub.nf.test.snap b/tests/test_build_stub.nf.test.snap
index a9bc48ee2..18d8ef8d1 100644
--- a/tests/test_build_stub.nf.test.snap
+++ b/tests/test_build_stub.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "stub test_build with fastp_trim": {
+    "stub test_build": {
         "content": [
             14,
             [
@@ -100,109 +100,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-28T21:05:36.346682866"
-    },
-    "stub test_build no fastp_trim": {
-        "content": [
-            14,
-            [
-                "bedops",
-                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
-                "gffread",
-                "gffread/Homo_sapiens.GRCh38.102.gff3",
-                "pipeline_info",
-                "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
-                "references/ensembl",
-                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
-                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
-                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
-                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
-                "references/fusion_report_db",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v102",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ref_annot.cdna.fa",
-                "rrnatranscripts",
-                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
-            ],
-            [
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.chr.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.genepred:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.refflat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "HGNC-DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-11-28T21:06:01.432893263"
+        "timestamp": "2024-11-29T17:29:03.231447837"
     }
 }
\ No newline at end of file

From 6e4f519567e77646bc18a3ef393ed8ef90bb0e5d Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 29 Nov 2024 21:22:29 +0000
Subject: [PATCH 111/887] refactor: `FUSIONREPORT_DOWNLOAD` accepts cosmic
 credentials via `ext.args`

---
 conf/modules.config                                        | 2 +-
 modules/local/fusionreport/download/main.nf                | 6 +-----
 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 2 +-
 workflows/build_references.nf                              | 4 ++--
 4 files changed, 5 insertions(+), 9 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 3bbe5ae7e..4390e5f7c 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -107,7 +107,7 @@ process {
     }
 
     withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : "" }
+        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : " --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         ext.args2        = { params.qiagen ? "--qiagen" : "" }
         publishDir = [
             path: { "${params.genomes_base}/fusion_report_db" },
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 6143dc052..acfcbf843 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -5,10 +5,6 @@ process FUSIONREPORT_DOWNLOAD {
     conda "bioconda::star=2.7.9a"
     container "docker.io/clinicalgenomics/fusion-report:3.1.0"
 
-    input:
-    val(username)
-    val(passwd)
-
     output:
     path "*"                , emit: reference
     path "versions.yml"     , emit: versions
@@ -17,7 +13,7 @@ process FUSIONREPORT_DOWNLOAD {
     def args = task.ext.args ?: ''
     def args2 = task.ext.args ?: ''
     """
-    fusion_report download --cosmic_usr "$username" --cosmic_passwd "$passwd" $args ./
+    fusion_report download $args ./
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 258c60500..a4aaf9c4b 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -158,7 +158,7 @@ def validateInputParameters() {
     genomeExistsError()
 
     if (params.build_references && params.no_cosmic) {
-            log.warn("No cosmic credentials were provided. Skipping `FUSIONREPORT_DOWNLOAD`")
+            log.warn("No cosmic credentials were provided. Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD`")
     }
 
 }
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 8abce2f49..def3c1627 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -76,8 +76,8 @@ workflow BUILD_REFERENCES {
     } else {
         UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.chrgtf)
     }
-    if ((params.fusionreport || params.all) && !params.no_cosmic) {
-        FUSIONREPORT_DOWNLOAD( params.cosmic_username, params.cosmic_passwd )
+    if (params.fusionreport || params.all) {
+        FUSIONREPORT_DOWNLOAD()
     }
 
 }

From 1649e77da8a6a1d4b2b7f0f0b86b72e64ebe4f56 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 29 Nov 2024 21:22:41 +0000
Subject: [PATCH 112/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 392f4ba5f..220496328 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -28,6 +28,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed some Nextflow run-commands in the docs [#491](https://github.com/nf-core/rnafusion/pull/491)
 - Fixed bug when trying to build indices behind a proxy and wget was unable to download arriba indices [#495](https://github.com/nf-core/rnafusion/issues/495)
 - Fixed bug in `FUSIONREPORT_DOWNLOAD` when building references with `--no_cosmic parameter` [#555](https://github.com/nf-core/rnafusion/issues/555)
+- Refactor structure in `FUSIONREPORT_DOWNLOAD` to use cosmic credentials in `ext.args` [#556](https://github.com/nf-core/rnafusion/issues/556)
 
 ### Removed
 

From 703b57ecfd0cc817882c190a872d6b40ee11e985 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 13:17:11 +0000
Subject: [PATCH 113/887] test: add nf-test to `FUSIONREPORT_DOWNLOAD`

---
 .../fusionreport/download/tests/main.nf.test  | 48 +++++++++++++
 .../download/tests/main.nf.test.snap          | 67 +++++++++++++++++++
 2 files changed, 115 insertions(+)
 create mode 100644 modules/local/fusionreport/download/tests/main.nf.test
 create mode 100644 modules/local/fusionreport/download/tests/main.nf.test.snap

diff --git a/modules/local/fusionreport/download/tests/main.nf.test b/modules/local/fusionreport/download/tests/main.nf.test
new file mode 100644
index 000000000..db8344b5a
--- /dev/null
+++ b/modules/local/fusionreport/download/tests/main.nf.test
@@ -0,0 +1,48 @@
+nextflow_process {
+
+    name "Test Process FUSIONREPORT_DOWNLOAD"
+    script "../main.nf"
+    process "FUSIONREPORT_DOWNLOAD"
+
+    test("Download fusionreport databases") {
+
+        when {
+            process {
+                """
+                // This process doesn't have any inputs
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.fusiongdb2,
+                    process.out.mitelman,
+                    process.out.versions
+                    ).match() }
+            )
+        }
+    }
+
+    test("Create stub files") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                // This process doesn't have any inputs
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/local/fusionreport/download/tests/main.nf.test.snap b/modules/local/fusionreport/download/tests/main.nf.test.snap
new file mode 100644
index 000000000..d828fcf51
--- /dev/null
+++ b/modules/local/fusionreport/download/tests/main.nf.test.snap
@@ -0,0 +1,67 @@
+{
+    "Download fusionreport databases": {
+        "content": [
+            [
+                "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a"
+            ],
+            [
+                "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a"
+            ],
+            [
+                "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-02T13:09:56.930477745"
+    },
+    "Create stub files": {
+        "content": [
+            {
+                "0": [
+                    "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "1": [
+                    "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "2": [
+                    "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "3": [
+                    "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "4": [
+                    "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "5": [
+                    "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
+                ],
+                "cosmic": [
+                    "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "fusiongdb2": [
+                    "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "log": [
+                    "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "mitelman": [
+                    "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "timestamp": [
+                    "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                ],
+                "versions": [
+                    "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-02T13:10:12.577952658"
+    }
+}
\ No newline at end of file

From 9e7fe540578bb7ded64a37553cadcadeb0e7a7c5 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 13:17:40 +0000
Subject: [PATCH 114/887] refactor: emit outputs separately

---
 modules/local/fusionreport/download/main.nf | 9 ++++++++-
 1 file changed, 8 insertions(+), 1 deletion(-)

diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index acfcbf843..183d95602 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -6,7 +6,11 @@ process FUSIONREPORT_DOWNLOAD {
     container "docker.io/clinicalgenomics/fusion-report:3.1.0"
 
     output:
-    path "*"                , emit: reference
+    path "fusiongdb2.db"    , emit: fusiongdb2
+    path "mitelman.db"      , emit: mitelman
+    path "cosmic.db"        , emit: cosmic, optional: true
+    path "*.txt"            , emit: timestamp
+    path "*.log"            , emit: log
     path "versions.yml"     , emit: versions
 
     script:
@@ -26,6 +30,9 @@ process FUSIONREPORT_DOWNLOAD {
     touch cosmic.db
     touch fusiongdb2.db
     touch mitelman.db
+    touch DB-timestamp.txt
+    touch fusion_report.log
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusion_report: \$(fusion_report --version | sed 's/fusion-report //')

From 8d0e6f749cf0ced57be6fd08c0e740d0282eb360 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 13:17:50 +0000
Subject: [PATCH 115/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 220496328..d1cbe2d1e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Option to avoid using COSMIC (for example in the case of clinical use) [547](https://github.com/nf-core/rnafusion/pull/547)
 - Add nf-test to nf-core module: `PICARD_COLLECTRNASEQMETRICS` and update module [#551](https://github.com/nf-core/rnafusion/pull/551)
 - Add `--skip_vcf` boolean parameter to skip vcf file generation [#554](https://github.com/nf-core/rnafusion/pull/554)
+- Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
 
 ### Changed
 

From 5b74d37fff61d6a23eb84825fdae521fc42e21d7 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 15:16:47 +0000
Subject: [PATCH 116/887] fix: use wildcard to get rrna gtf

---
 modules.json                                  |  3 ++-
 modules/nf-core/rrnatranscripts/main.nf       |  2 +-
 .../rrnatranscripts/rrnatranscripts.diff      | 21 +++++++++++++++++++
 3 files changed, 24 insertions(+), 2 deletions(-)
 create mode 100644 modules/nf-core/rrnatranscripts/rrnatranscripts.diff

diff --git a/modules.json b/modules.json
index e3709392d..66710aa74 100644
--- a/modules.json
+++ b/modules.json
@@ -88,7 +88,8 @@
                     "rrnatranscripts": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/rrnatranscripts/rrnatranscripts.diff"
                     },
                     "salmon/index": {
                         "branch": "master",
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
index 50843c768..e5d918473 100644
--- a/modules/nf-core/rrnatranscripts/main.nf
+++ b/modules/nf-core/rrnatranscripts/main.nf
@@ -20,7 +20,7 @@ process RRNATRANSCRIPTS {
     script:
     def prefix = task.ext.prefix ?: "${gtf.baseName}"
     """
-    grep -E '^#|rRNA' genome.gtf > ${prefix}_rrna_intervals.gtf || true
+    grep -E '^#|rRNA' *.gtf > ${prefix}_rrna_intervals.gtf || true
     if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
         rm ${prefix}_rrna_intervals.gtf
     fi
diff --git a/modules/nf-core/rrnatranscripts/rrnatranscripts.diff b/modules/nf-core/rrnatranscripts/rrnatranscripts.diff
new file mode 100644
index 000000000..33bb1910a
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/rrnatranscripts.diff
@@ -0,0 +1,21 @@
+Changes in module 'nf-core/rrnatranscripts'
+'modules/nf-core/rrnatranscripts/environment.yml' is unchanged
+Changes in 'rrnatranscripts/main.nf':
+--- modules/nf-core/rrnatranscripts/main.nf
++++ modules/nf-core/rrnatranscripts/main.nf
+@@ -20,7 +20,7 @@
+     script:
+     def prefix = task.ext.prefix ?: "${gtf.baseName}"
+     """
+-    grep -E '^#|rRNA' genome.gtf > ${prefix}_rrna_intervals.gtf || true
++    grep -E '^#|rRNA' *.gtf > ${prefix}_rrna_intervals.gtf || true
+     if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
+         rm ${prefix}_rrna_intervals.gtf
+     fi
+
+'modules/nf-core/rrnatranscripts/meta.yml' is unchanged
+'modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py' is unchanged
+'modules/nf-core/rrnatranscripts/tests/main.nf.test' is unchanged
+'modules/nf-core/rrnatranscripts/tests/main.nf.test.snap' is unchanged
+'modules/nf-core/rrnatranscripts/tests/tags.yml' is unchanged
+************************************************************

From a21107768fa5e4c7f4a4623cce8b61148f306f91 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 15:17:04 +0000
Subject: [PATCH 117/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 220496328..e485670f6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -29,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed bug when trying to build indices behind a proxy and wget was unable to download arriba indices [#495](https://github.com/nf-core/rnafusion/issues/495)
 - Fixed bug in `FUSIONREPORT_DOWNLOAD` when building references with `--no_cosmic parameter` [#555](https://github.com/nf-core/rnafusion/issues/555)
 - Refactor structure in `FUSIONREPORT_DOWNLOAD` to use cosmic credentials in `ext.args` [#556](https://github.com/nf-core/rnafusion/issues/556)
+- Fixed bug in nf-core `RRNATRANSCRIPTS` module [#563](https://github.com/nf-core/rnafusion/issues/563)
 
 ### Removed
 

From 52108b6b95cbf76deccd551f41fd4d036f22415f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 15:51:13 +0000
Subject: [PATCH 118/887] fix: add gffread config

---
 conf/modules.config | 4 ++++
 1 file changed, 4 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 4390e5f7c..abe0e79d4 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -18,6 +18,10 @@ process {
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
+    withName: GFFREAD {
+        ext.args = '-w -S'
+    }
+
     withName: 'ARRIBA_ARRIBA' {
         publishDir = [
             path: { "${params.outdir}/arriba" },

From 4b19e5aa943d1500412c9cd8184400a2ab8a9476 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 15:51:22 +0000
Subject: [PATCH 119/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 220496328..175699cc1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -29,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed bug when trying to build indices behind a proxy and wget was unable to download arriba indices [#495](https://github.com/nf-core/rnafusion/issues/495)
 - Fixed bug in `FUSIONREPORT_DOWNLOAD` when building references with `--no_cosmic parameter` [#555](https://github.com/nf-core/rnafusion/issues/555)
 - Refactor structure in `FUSIONREPORT_DOWNLOAD` to use cosmic credentials in `ext.args` [#556](https://github.com/nf-core/rnafusion/issues/556)
+- Fixed bug in `GFFREAD` that caused output `gffread_fasta` not being produced [#565](https://github.com/nf-core/rnafusion/issues/565)
 
 ### Removed
 

From 96f6f9f97f09045bcbc6f5fc5cb3f11f4270975e Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 2 Dec 2024 18:08:55 +0000
Subject: [PATCH 120/887] fix: update fix from nf-core/modules

---
 modules.json                                  |  2 +-
 modules/nf-core/rrnatranscripts/main.nf       |  4 ++--
 .../rrnatranscripts/rrnatranscripts.diff      | 21 -------------------
 3 files changed, 3 insertions(+), 24 deletions(-)
 delete mode 100644 modules/nf-core/rrnatranscripts/rrnatranscripts.diff

diff --git a/modules.json b/modules.json
index 66710aa74..15c6ecac1 100644
--- a/modules.json
+++ b/modules.json
@@ -87,7 +87,7 @@
                     },
                     "rrnatranscripts": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "812edf8cf702de42d2d8c7314d6f03b97e20abeb",
                         "installed_by": ["modules"],
                         "patch": "modules/nf-core/rrnatranscripts/rrnatranscripts.diff"
                     },
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
index e5d918473..982c53cd4 100644
--- a/modules/nf-core/rrnatranscripts/main.nf
+++ b/modules/nf-core/rrnatranscripts/main.nf
@@ -1,5 +1,5 @@
 process RRNATRANSCRIPTS {
-    tag '$rrna'
+    tag "$gtf"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
@@ -20,7 +20,7 @@ process RRNATRANSCRIPTS {
     script:
     def prefix = task.ext.prefix ?: "${gtf.baseName}"
     """
-    grep -E '^#|rRNA' *.gtf > ${prefix}_rrna_intervals.gtf || true
+    grep -E '^#|rRNA' ${gtf} > ${prefix}_rrna_intervals.gtf || true
     if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
         rm ${prefix}_rrna_intervals.gtf
     fi
diff --git a/modules/nf-core/rrnatranscripts/rrnatranscripts.diff b/modules/nf-core/rrnatranscripts/rrnatranscripts.diff
deleted file mode 100644
index 33bb1910a..000000000
--- a/modules/nf-core/rrnatranscripts/rrnatranscripts.diff
+++ /dev/null
@@ -1,21 +0,0 @@
-Changes in module 'nf-core/rrnatranscripts'
-'modules/nf-core/rrnatranscripts/environment.yml' is unchanged
-Changes in 'rrnatranscripts/main.nf':
---- modules/nf-core/rrnatranscripts/main.nf
-+++ modules/nf-core/rrnatranscripts/main.nf
-@@ -20,7 +20,7 @@
-     script:
-     def prefix = task.ext.prefix ?: "${gtf.baseName}"
-     """
--    grep -E '^#|rRNA' genome.gtf > ${prefix}_rrna_intervals.gtf || true
-+    grep -E '^#|rRNA' *.gtf > ${prefix}_rrna_intervals.gtf || true
-     if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
-         rm ${prefix}_rrna_intervals.gtf
-     fi
-
-'modules/nf-core/rrnatranscripts/meta.yml' is unchanged
-'modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py' is unchanged
-'modules/nf-core/rrnatranscripts/tests/main.nf.test' is unchanged
-'modules/nf-core/rrnatranscripts/tests/main.nf.test.snap' is unchanged
-'modules/nf-core/rrnatranscripts/tests/tags.yml' is unchanged
-************************************************************

From a56eb9760eb5f34f21c0dba0f6dfc8f408a4ff71 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 3 Dec 2024 12:16:41 +0000
Subject: [PATCH 121/887] test: add config to fusionreport nf-test

---
 modules/local/fusionreport/download/tests/main.nf.test    | 1 +
 modules/local/fusionreport/download/tests/nextflow.config | 5 +++++
 2 files changed, 6 insertions(+)
 create mode 100644 modules/local/fusionreport/download/tests/nextflow.config

diff --git a/modules/local/fusionreport/download/tests/main.nf.test b/modules/local/fusionreport/download/tests/main.nf.test
index db8344b5a..33486764a 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test
+++ b/modules/local/fusionreport/download/tests/main.nf.test
@@ -5,6 +5,7 @@ nextflow_process {
     process "FUSIONREPORT_DOWNLOAD"
 
     test("Download fusionreport databases") {
+        config './nextflow.config'
 
         when {
             process {
diff --git a/modules/local/fusionreport/download/tests/nextflow.config b/modules/local/fusionreport/download/tests/nextflow.config
new file mode 100644
index 000000000..7c1ffb507
--- /dev/null
+++ b/modules/local/fusionreport/download/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'FUSIONREPORT_DOWNLOAD' {
+        ext.args         =  "--no-cosmic"
+    }
+}

From 2be6ceb2eee4e657c33dff5d7e9de2274b5305b9 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Tue, 3 Dec 2024 13:24:04 +0000
Subject: [PATCH 122/887] Added test_build test

---
 modules/nf-core/rrnatranscripts/main.nf |  2 +-
 nf-test.config                          |  2 +-
 tests/.nftignore                        |  2 ++
 tests/test_build.nf.test.snap           | 40 +++++++++++++++++++++++++
 4 files changed, 44 insertions(+), 2 deletions(-)
 create mode 100644 tests/test_build.nf.test.snap

diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
index 50843c768..461c09331 100644
--- a/modules/nf-core/rrnatranscripts/main.nf
+++ b/modules/nf-core/rrnatranscripts/main.nf
@@ -1,5 +1,5 @@
 process RRNATRANSCRIPTS {
-    tag '$rrna'
+    tag "$gtf"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
diff --git a/nf-test.config b/nf-test.config
index cefd5a626..16f182d49 100644
--- a/nf-test.config
+++ b/nf-test.config
@@ -6,7 +6,7 @@ config {
     workDir ".nf-test"
 
     // location of library folder that is added automatically to the classpath
-    libDir "tests/pipeline/lib/"
+    libDir "tests/lib/"
 
     // location of an optional nextflow.config file specific for executing tests
     configFile "nextflow.config"
diff --git a/tests/.nftignore b/tests/.nftignore
index d70cc56e9..54792ea8f 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -1,3 +1,5 @@
 pipeline_info/*.{html,json,txt,yml}
 references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
 references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
+references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa
+references/hgnc/HGNC-DB-timestamp.txt
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
new file mode 100644
index 000000000..839fcb30d
--- /dev/null
+++ b/tests/test_build.nf.test.snap
@@ -0,0 +1,40 @@
+{
+    "test_build": {
+        "content": [
+            [
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
+                "gffread",
+                "gffread/Homo_sapiens.GRCh38.102.gff3",
+                "pipeline_info",
+                "references",
+                "references/ensembl",
+                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
+                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
+                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "rrnatranscripts"
+            ],
+            [
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,092f1bf29fce906ff7b5ece02b4b21c8",
+                "Homo_sapiens.GRCh38.102.gff3:md5,f559ec7a1e361351b97f4856ddfcbf84",
+                "Homo_sapiens.GRCh38.102.chr.gtf:md5,0069687307852c63596b4d2ebdbbaf0c",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d527f3eb6b664020cf4d882b5820056f",
+                "Homo_sapiens.GRCh38.102.genepred:md5,59c577ca4ab033c0ce1feac1e387bcab",
+                "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
+                "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
+                "hgnc_complete_set.txt:md5,8c7ac03b6fd965379e081b467170b0fc"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-03T13:14:36.201169164"
+    }
+}
\ No newline at end of file

From 144c78056ed8fd12ac8bb941901a396db72e33b7 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Tue, 3 Dec 2024 13:57:31 +0000
Subject: [PATCH 123/887] Update snaps

---
 tests/test_build.nf.test.snap      | 4 ++--
 tests/test_build_stub.nf.test.snap | 4 +---
 tests/test_stub.nf.test.snap       | 4 ++--
 3 files changed, 5 insertions(+), 7 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 839fcb30d..9318d4dc1 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -28,13 +28,13 @@
                 "Homo_sapiens.GRCh38.102.genepred:md5,59c577ca4ab033c0ce1feac1e387bcab",
                 "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
                 "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
-                "hgnc_complete_set.txt:md5,8c7ac03b6fd965379e081b467170b0fc"
+                "hgnc_complete_set.txt:md5,34f4bd879d972e511fcb0318df9775b6"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T13:14:36.201169164"
+        "timestamp": "2024-12-03T13:45:13.303642069"
     }
 }
\ No newline at end of file
diff --git a/tests/test_build_stub.nf.test.snap b/tests/test_build_stub.nf.test.snap
index 18d8ef8d1..b59db3799 100644
--- a/tests/test_build_stub.nf.test.snap
+++ b/tests/test_build_stub.nf.test.snap
@@ -64,7 +64,6 @@
                 "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Homo_sapiens.GRCh38.102.chr.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Homo_sapiens.GRCh38.102.genepred:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Homo_sapiens.GRCh38.102.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -74,7 +73,6 @@
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "HGNC-DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -100,6 +98,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-29T17:29:03.231447837"
+        "timestamp": "2024-12-03T13:46:03.887799524"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 63dc16109..7731abfef 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -212,7 +212,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-28T21:25:48.332933141"
+        "timestamp": "2024-12-03T13:46:54.238312636"
     },
     "stub test with fastp trim": {
         "content": [
@@ -449,6 +449,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-11-28T21:06:29.861338628"
+        "timestamp": "2024-12-03T13:46:30.067018094"
     }
 }
\ No newline at end of file

From b06bc4e6e02eb5a51e11109f470de5cd6b539a4d Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Tue, 3 Dec 2024 15:04:58 +0000
Subject: [PATCH 124/887] Updated rrnatranscripts

---
 modules.json                            |  2 +-
 modules/nf-core/rrnatranscripts/main.nf |  2 +-
 tests/test_build.nf.test.snap           | 13 ++++++++++---
 3 files changed, 12 insertions(+), 5 deletions(-)

diff --git a/modules.json b/modules.json
index e3709392d..13b87c2e9 100644
--- a/modules.json
+++ b/modules.json
@@ -87,7 +87,7 @@
                     },
                     "rrnatranscripts": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "812edf8cf702de42d2d8c7314d6f03b97e20abeb",
                         "installed_by": ["modules"]
                     },
                     "salmon/index": {
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
index 461c09331..982c53cd4 100644
--- a/modules/nf-core/rrnatranscripts/main.nf
+++ b/modules/nf-core/rrnatranscripts/main.nf
@@ -20,7 +20,7 @@ process RRNATRANSCRIPTS {
     script:
     def prefix = task.ext.prefix ?: "${gtf.baseName}"
     """
-    grep -E '^#|rRNA' genome.gtf > ${prefix}_rrna_intervals.gtf || true
+    grep -E '^#|rRNA' ${gtf} > ${prefix}_rrna_intervals.gtf || true
     if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
         rm ${prefix}_rrna_intervals.gtf
     fi
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 9318d4dc1..df97d56f4 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -2,6 +2,8 @@
     "test_build": {
         "content": [
             [
+                "bedops",
+                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
                 "gffread",
@@ -15,12 +17,15 @@
                 "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
                 "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
                 "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
+                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
                 "references/hgnc",
                 "references/hgnc/HGNC-DB-timestamp.txt",
                 "references/hgnc/hgnc_complete_set.txt",
-                "rrnatranscripts"
+                "rrnatranscripts",
+                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
             ],
             [
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,5bc3ccc76735ae46699a75269f0ea65b",
                 "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,092f1bf29fce906ff7b5ece02b4b21c8",
                 "Homo_sapiens.GRCh38.102.gff3:md5,f559ec7a1e361351b97f4856ddfcbf84",
                 "Homo_sapiens.GRCh38.102.chr.gtf:md5,0069687307852c63596b4d2ebdbbaf0c",
@@ -28,13 +33,15 @@
                 "Homo_sapiens.GRCh38.102.genepred:md5,59c577ca4ab033c0ce1feac1e387bcab",
                 "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
                 "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
-                "hgnc_complete_set.txt:md5,34f4bd879d972e511fcb0318df9775b6"
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,0abf61877f65247b15c438d605d85599",
+                "hgnc_complete_set.txt:md5,34f4bd879d972e511fcb0318df9775b6",
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,744bf505deb50837b15441e808cad345"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T13:45:13.303642069"
+        "timestamp": "2024-12-03T15:04:03.186303848"
     }
 }
\ No newline at end of file

From 2519d663a5f6e5977a1849576291b3a71f60d072 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Tue, 3 Dec 2024 16:46:16 +0000
Subject: [PATCH 125/887] Use latest-stable instead of latest-everything in CI

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 2cc8fba44..1c8832e2f 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -36,7 +36,7 @@ jobs:
       matrix:
         NXF_VER:
           - "24.04.2"
-          - "latest-everything"
+          - "latest-stable"
         test_profile:
           - "test_stub"
           - "test_build_stub"

From b4ff921ed1632934df7f4d3d27d7db639f20e54a Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 3 Dec 2024 17:39:03 +0000
Subject: [PATCH 126/887] test: add latest-stable to fix issues in ci

---
 .github/workflows/ci.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 2751a4760..251f50340 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -28,7 +28,7 @@ jobs:
       matrix:
         NXF_VER:
           - "24.04.2"
-          - "latest-everything"
+          - "latest-stable"
         trim_parameters:
           - "--fastp_trim false"
           - "--fastp_trim true"

From bd419d4be7373d66afde4839504151ec866d3168 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Tue, 3 Dec 2024 19:01:30 +0000
Subject: [PATCH 127/887] Addressing PR comments

---
 .github/workflows/ci.yml                      |   1 -
 conf/test_build.config                        |   6 +-
 conf/test_full.config                         |   4 +
 modules/local/fusioninspector/environment.yml |  10 ++
 modules/local/fusioninspector/main.nf         |   1 -
 .../local/starfusion/build/environment.yml    |  10 ++
 modules/local/starfusion/build/main.nf        |   1 -
 .../local/starfusion/detect/environment.yml   |  10 ++
 modules/local/starfusion/detect/main.nf       |   1 -
 .../local/starfusion/download/environment.yml |  10 ++
 modules/local/starfusion/download/main.nf     |   1 -
 tests/test.nf.test                            |  73 -------------
 tests/test_build.nf.test                      |   1 -
 tests/test_build_stub.nf.test                 |  41 -------
 tests/test_build_stub.nf.test.snap            | 103 ------------------
 tests/test_cosmic.nf.test                     |   5 +-
 tests/test_stub.nf.test                       |  13 +--
 17 files changed, 53 insertions(+), 238 deletions(-)
 create mode 100644 modules/local/fusioninspector/environment.yml
 create mode 100644 modules/local/starfusion/build/environment.yml
 create mode 100644 modules/local/starfusion/detect/environment.yml
 create mode 100644 modules/local/starfusion/download/environment.yml
 delete mode 100644 tests/test.nf.test
 delete mode 100644 tests/test_build_stub.nf.test
 delete mode 100644 tests/test_build_stub.nf.test.snap

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 1c8832e2f..71ff72459 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -39,7 +39,6 @@ jobs:
           - "latest-stable"
         test_profile:
           - "test_stub"
-          - "test_build_stub"
           - "test_build"
         compute_profile:
           - "docker"
diff --git a/conf/test_build.config b/conf/test_build.config
index 32ee1bb62..02ca2a170 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -23,6 +23,8 @@ params {
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
     // Input data
-    build_references             = true
-    input                        = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
+    build_references           = true
+    input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
+    no_cosmic                  = true
+    all                        = true
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index 467445604..cfcb7865b 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -15,4 +15,8 @@ params {
     // Input data for full size test
     input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
     all   = true
+    // TODO
+    // test_full can't run currently because the references must be given and they are not available.
+    // This profile should be updated once they get uploaded.
+
     }
diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
new file mode 100644
index 000000000..06a9828af
--- /dev/null
+++ b/modules/local/fusioninspector/environment.yml
@@ -0,0 +1,10 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::star-fusion=1.7.0
+  - bioconda::trinity=2.15.2
+  - bioconda::samtools=1.21
+  - bioconda::star=2.7.11b
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 0f9f0abb0..59d513fbb 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -2,7 +2,6 @@ process FUSIONINSPECTOR {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
new file mode 100644
index 000000000..06a9828af
--- /dev/null
+++ b/modules/local/starfusion/build/environment.yml
@@ -0,0 +1,10 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::star-fusion=1.7.0
+  - bioconda::trinity=2.15.2
+  - bioconda::samtools=1.21
+  - bioconda::star=2.7.11b
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index e7b969a2f..cedf8c461 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -1,7 +1,6 @@
 process STARFUSION_BUILD {
     tag 'star-fusion'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
new file mode 100644
index 000000000..06a9828af
--- /dev/null
+++ b/modules/local/starfusion/detect/environment.yml
@@ -0,0 +1,10 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::star-fusion=1.7.0
+  - bioconda::trinity=2.15.2
+  - bioconda::samtools=1.21
+  - bioconda::star=2.7.11b
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index a3a433652..3ed54af2d 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -2,7 +2,6 @@ process STARFUSION {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
diff --git a/modules/local/starfusion/download/environment.yml b/modules/local/starfusion/download/environment.yml
new file mode 100644
index 000000000..06a9828af
--- /dev/null
+++ b/modules/local/starfusion/download/environment.yml
@@ -0,0 +1,10 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::star-fusion=1.7.0
+  - bioconda::trinity=2.15.2
+  - bioconda::samtools=1.21
+  - bioconda::star=2.7.11b
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index a486b607e..250f13967 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,7 +1,6 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
-    conda "bioconda::dfam=3.7 bioconda::hmmer=3.4 bioconda::star-fusion=1.7.0 bioconda::trinity=2.15.2 bioconda::samtools=1.21 bioconda::star=2.7.11b"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     output:
diff --git a/tests/test.nf.test b/tests/test.nf.test
deleted file mode 100644
index be6ab52c2..000000000
--- a/tests/test.nf.test
+++ /dev/null
@@ -1,73 +0,0 @@
-nextflow_pipeline {
-
-    name "Test pipeline"
-    script "../main.nf"
-    profile "test"
-    tag "pipeline"
-    tag "pipeline_rnafusion"
-    tag "test"
-
-    test("test with fastp trim") {
-
-        when {
-            params {
-                outdir     = "$outputDir"
-                fastp_trim = true
-                no_cosmic  = true
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-
-    test("test no fastp trim") {
-
-        when {
-            params {
-                outdir     = "$outputDir"
-                fastp_trim = false
-                no_cosmic  = true
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-}
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index a800b0e6b..1c0830deb 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -12,7 +12,6 @@ nextflow_pipeline {
         when {
             params {
                 outdir = "$outputDir"
-                no_cosmic  = true
             }
         }
 
diff --git a/tests/test_build_stub.nf.test b/tests/test_build_stub.nf.test
deleted file mode 100644
index 3235720ac..000000000
--- a/tests/test_build_stub.nf.test
+++ /dev/null
@@ -1,41 +0,0 @@
-nextflow_pipeline {
-
-    name "Test pipeline"
-    script "../main.nf"
-    profile "test_build"
-    options "-stub" // TODO remove once the pipeline is fixed
-    tag "pipeline"
-    tag "pipeline_rnafusion"
-    tag "test_build_stub"
-
-    test("stub test_build") {
-
-        when {
-            params {
-                outdir          = "$outputDir"
-                cosmic_username = "user" // placeholder string to avoid null input val in process
-                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
-                all             = true
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-}
diff --git a/tests/test_build_stub.nf.test.snap b/tests/test_build_stub.nf.test.snap
deleted file mode 100644
index b59db3799..000000000
--- a/tests/test_build_stub.nf.test.snap
+++ /dev/null
@@ -1,103 +0,0 @@
-{
-    "stub test_build": {
-        "content": [
-            14,
-            [
-                "bedops",
-                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
-                "gffread",
-                "gffread/Homo_sapiens.GRCh38.102.gff3",
-                "pipeline_info",
-                "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
-                "references/ensembl",
-                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
-                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
-                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
-                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
-                "references/fusion_report_db",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v102",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ref_annot.cdna.fa",
-                "rrnatranscripts",
-                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
-            ],
-            [
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.chr.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.genepred:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102.refflat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-03T13:46:03.887799524"
-    }
-}
\ No newline at end of file
diff --git a/tests/test_cosmic.nf.test b/tests/test_cosmic.nf.test
index ffa1e4896..9a56e76e5 100644
--- a/tests/test_cosmic.nf.test
+++ b/tests/test_cosmic.nf.test
@@ -43,7 +43,6 @@ nextflow_pipeline {
         when {
             params {
                 outdir     = "$outputDir"
-                fastp_trim = false
             }
         }
 
@@ -73,8 +72,8 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir     = "$outputDir"
-                fastp_trim = false
+                outdir           = "$outputDir"
+                build_references = true
             }
         }
 
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 90d9bb647..351412a47 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -3,7 +3,7 @@ nextflow_pipeline {
     name "Test pipeline"
     script "../main.nf"
     profile "test"
-    options "-stub" // TODO remove once the pipeline is fixed
+    options "-stub" // TODO remove once reference files are available
     tag "pipeline"
     tag "pipeline_rnafusion"
     tag "test_stub"
@@ -14,9 +14,6 @@ nextflow_pipeline {
             params {
                 outdir          = "$outputDir"
                 fastp_trim      = true
-                cosmic_username = "user" // placeholder string to avoid null input val in process
-                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
-                all             = true
             }
         }
 
@@ -25,7 +22,7 @@ nextflow_pipeline {
             // Ignore files with timestamps in their names
             def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
             // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
             assertAll(
                 { assert workflow.success},
                 { assert snapshot(
@@ -36,7 +33,7 @@ nextflow_pipeline {
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
-                    stable_path
+                    // stable_path // TODO uncomment once -stub is removed
                 ).match() }
             )
         }
@@ -47,10 +44,6 @@ nextflow_pipeline {
         when {
             params {
                 outdir          = "$outputDir"
-                fastp_trim      = false
-                cosmic_username = "user" // placeholder string to avoid null input val in process
-                cosmic_passwd   = "password" // placeholder string to avoid null input val in process
-                all             = true
             }
         }
 

From 0f88731f8e8c5af8254dc0f4cbb1a3d8eb8c34bd Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Tue, 3 Dec 2024 19:31:05 +0000
Subject: [PATCH 128/887] Updated snaps

---
 tests/test_build.nf.test      |   1 +
 tests/test_build.nf.test.snap |   2 +-
 tests/test_stub.nf.test       |   4 +-
 tests/test_stub.nf.test.snap  | 361 +---------------------------------
 4 files changed, 10 insertions(+), 358 deletions(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 1c0830deb..1999a3b47 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -12,6 +12,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir = "$outputDir"
+                all    = false // Output files when running all are too big for CI
             }
         }
 
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index df97d56f4..0833ced64 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -42,6 +42,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T15:04:03.186303848"
+        "timestamp": "2024-12-03T19:26:00.897559313"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 351412a47..41c7194f0 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -52,7 +52,7 @@ nextflow_pipeline {
             // Ignore files with timestamps in their names
             def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
             // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
             assertAll(
                 { assert workflow.success},
                 { assert snapshot(
@@ -63,7 +63,7 @@ nextflow_pipeline {
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
-                    stable_path
+                    // stable_path // TODO uncomment once -stub is removed
                 ).match() }
             )
         }
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 7731abfef..3b83c2400 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,67 +1,22 @@
 {
     "stub test no fastp trim": {
         "content": [
-            15,
+            4,
             {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER": {
-                    "fusioncatcher": 1.33
-                },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
                 },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.2.0-1-g3948afb6b"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SAMTOOLS_INDEX_FOR_STARFUSION": {
-                    "samtools": 1.21
-                },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.7.0"
-                },
-                "STAR_FOR_ARRIBA": {
-                    "star": "2.7.10a",
-                    "samtools": 1.18,
-                    "gawk": "5.1.0"
-                },
-                "STAR_FOR_STARFUSION": {
-                    "star": "2.7.10a",
-                    "samtools": 1.18,
-                    "gawk": "5.1.0"
-                },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.1"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "fusioncatcher",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -74,153 +29,24 @@
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv",
-                "stringtie",
-                "stringtie/[:]",
-                "stringtie/[:]/stringtie.merged.gtf",
-                "stringtie/test.ballgown",
-                "stringtie/test.coverage.gtf",
-                "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
-            ],
-            [
-                "test.arriba.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
-                "test.arriba.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
-                "test.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusioncatcher.fusion-genes.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusioncatcher.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusioncatcher.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "AAA_BBB.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusionreport.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusionreport_filtered.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusions.csv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusions.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "multiqc_report.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.bam.metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_collectinsertsize.pdf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_collectinsertsize.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_meta_info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "stringtie.merged.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                "salmon/test_meta_info.json"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T13:46:54.238312636"
+        "timestamp": "2024-12-03T19:27:00.717876194"
     },
     "stub test with fastp trim": {
         "content": [
-            17,
+            6,
             {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
                 "FASTP": {
                     "fastp": "0.23.4"
                 },
@@ -230,52 +56,14 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER": {
-                    "fusioncatcher": 1.33
-                },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
                 },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.2.0-1-g3948afb6b"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SAMTOOLS_INDEX_FOR_STARFUSION": {
-                    "samtools": 1.21
-                },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.7.0"
-                },
-                "STAR_FOR_ARRIBA": {
-                    "star": "2.7.10a",
-                    "samtools": 1.18,
-                    "gawk": "5.1.0"
-                },
-                "STAR_FOR_STARFUSION": {
-                    "star": "2.7.10a",
-                    "samtools": 1.18,
-                    "gawk": "5.1.0"
-                },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.1"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -288,10 +76,6 @@
                 "fastqc_for_fastp",
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
-                "fusioncatcher",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -304,151 +88,18 @@
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv",
-                "stringtie",
-                "stringtie/[:]",
-                "stringtie/[:]/stringtie.merged.gtf",
-                "stringtie/test.ballgown",
-                "stringtie/test.coverage.gtf",
-                "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
-            ],
-            [
-                "test.arriba.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
-                "test.arriba.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260",
-                "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "test.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_trimmed.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_trimmed.zip:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusioncatcher.fusion-genes.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusioncatcher.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusioncatcher.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "AAA_BBB.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusionreport.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusionreport_filtered.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusions.csv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusions.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "multiqc_report.html:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.bam.metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_collectinsertsize.pdf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_collectinsertsize.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_meta_info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.unsort.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Chimeric.out.junction:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.ReadsPerGene.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.bg:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Signal.UniqueMultiple.str1.out.wig:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.out.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.toTranscriptome.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.unmapped_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "test.unmapped_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "testXd.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "stringtie.merged.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                "salmon/test_meta_info.json"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T13:46:30.067018094"
+        "timestamp": "2024-12-03T19:26:43.375635514"
     }
 }
\ No newline at end of file

From 4b607dd907794888f7d3e0d717c59d68d5ea6fdd Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Tue, 3 Dec 2024 19:56:37 +0000
Subject: [PATCH 129/887] change module structure

---
 subworkflows/local/qc_workflow/main.nf        |  40 ++++++
 .../local/qc_workflow/test/main.nf.test       | 133 ++++++++++++++++++
 .../local/qc_workflow/test/nextflow.config    |  10 ++
 3 files changed, 183 insertions(+)
 create mode 100644 subworkflows/local/qc_workflow/main.nf
 create mode 100644 subworkflows/local/qc_workflow/test/main.nf.test
 create mode 100644 subworkflows/local/qc_workflow/test/nextflow.config

diff --git a/subworkflows/local/qc_workflow/main.nf b/subworkflows/local/qc_workflow/main.nf
new file mode 100644
index 000000000..a8473d446
--- /dev/null
+++ b/subworkflows/local/qc_workflow/main.nf
@@ -0,0 +1,40 @@
+//
+// Check input samplesheet and get read channels
+//
+
+include { PICARD_COLLECTRNASEQMETRICS       } from '../../../modules/nf-core/picard/collectrnaseqmetrics'
+include { GATK4_MARKDUPLICATES              } from '../../../modules/nf-core/gatk4/markduplicates'
+include { PICARD_COLLECTINSERTSIZEMETRICS   } from '../../../modules/nf-core/picard/collectinsertsizemetrics'
+
+workflow QC_WORKFLOW {
+    take:
+        ch_bam_sorted           // channel [ meta, bam        ]
+        ch_chrgtf               // channel [ meta, gtf        ]
+        ch_refflat              // channel [ meta, refflat    ]
+        ch_fasta                // channel [ meta, fasta      ]
+        ch_fai                  // channel [ meta, fai        ]
+        ch_rrna_interval        // channel [ meta, interval   ]
+
+    main:
+        ch_versions = Channel.empty()
+
+        PICARD_COLLECTRNASEQMETRICS(ch_bam_sorted, ch_refflat.map{ meta, refflat -> [ refflat ] }, ch_fasta.map{ meta, fasta -> [ fasta ] }, ch_rrna_interval.map{ meta, intervals -> [ intervals ] }.ifEmpty([]) ) // Some chromosome or annotation may not have rRNA genes
+        ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
+        ch_rnaseq_metrics = PICARD_COLLECTRNASEQMETRICS.out.metrics
+
+        GATK4_MARKDUPLICATES(ch_bam_sorted, ch_fasta.map { meta, fasta -> [ fasta ]}, ch_fai.map { meta, fasta_fai -> [ fasta_fai ]})
+        ch_versions = ch_versions.mix(GATK4_MARKDUPLICATES.out.versions)
+        ch_duplicate_metrics = GATK4_MARKDUPLICATES.out.metrics
+
+        PICARD_COLLECTINSERTSIZEMETRICS(ch_bam_sorted)
+        ch_versions = ch_versions.mix(PICARD_COLLECTINSERTSIZEMETRICS.out.versions)
+        ch_insertsize_metrics = PICARD_COLLECTINSERTSIZEMETRICS.out.metrics
+
+    emit:
+        versions            = ch_versions
+        rnaseq_metrics      = ch_rnaseq_metrics
+        duplicate_metrics   = ch_duplicate_metrics
+        insertsize_metrics  = ch_insertsize_metrics
+
+}
+
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
new file mode 100644
index 000000000..57dd38da5
--- /dev/null
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -0,0 +1,133 @@
+nextflow_workflow {
+
+    name "Test Subworkflow QC_WORKFLOW"
+    script "../main.nf"
+    config "./nextflow.config"
+    workflow "QC_WORKFLOW"
+    tag "qc"
+    tag "subworkflow"
+
+    test("QC_WORKFLOW - Homo sapiens chr22") {
+
+        // Generate refflat file
+        setup {
+
+            // Create refflat reference
+            run("UCSC_GTFTOGENEPRED") {
+                script "../../../../modules/nf-core/ucsc/gtftogenepred/main.nf"
+                process {
+                    """
+                    input[0] =
+                        Channel.fromPath(
+                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf",
+                            checkIfExists: true
+                        )
+                        .map{ [ [id:it.Name], it ] }
+                    """
+                }
+            }
+
+            // Filter GTF to extract rRNA genes
+            run("RRNATRANSCRIPTS") {
+                script "../../../../modules/nf-core/rrnatranscripts/main.nf"
+                process {
+                    """
+                    input[0] = Channel.fromPath("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
+                    """
+                }
+            }
+
+            // Convert rRNA GTF to BED
+            run("BEDOPS_CONVERT2BED") {
+                script "../../../../modules/nf-core/bedops/convert2bed/main.nf"
+                process {
+                    """
+                    input[0] = RRNATRANSCRIPTS.out.rrna_gtf.map{ it -> [ [id:it.Name], it ] }
+                    """
+                }
+            }
+
+            // Convert rRNA BED to interval list (the necessary file)
+            run("GATK4_BEDTOINTERVALLIST") {
+                script "../../../../modules/nf-core/gatk4/bedtointervallist/main.nf"
+                process {
+                    """
+                    input[0] = BEDOPS_CONVERT2BED.out.bed
+                    input[1] = Channel.of(
+                        [
+                            [id: 'chr22_dic'],
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.dic", checkIfExist: true)
+                        ]
+                    )
+                    """
+                }
+            }
+
+        }
+
+        when {
+            // Params to activate modules ext.when condition
+            params {
+                skip_qc              = false
+                fusioninspector_only = false
+                starfusion           = true
+                all                  = true
+            }
+
+            workflow {
+                """
+                // ch_bam_sorted
+                input[0] = Channel.of(
+                    [
+                        [id: "chr22_bam"],
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
+                    ])
+
+                // ch_bam_sorted_indexed
+                input[1] = Channel.of(
+                    [
+                        [id: "chr22_bam"],
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true),
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai", checkIfExists: true)
+                    ])
+
+                // ch_chrgtf
+                input[2] = Channel.of(
+                    [
+                        [ id: "chr22_gtf" ],
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
+                    ])
+
+                // ch_refflat
+                input[3] = UCSC_GTFTOGENEPRED.out.refflat
+
+                // ch_fasta
+                input[4] = Channel.of(
+                    [
+                        [ id: "test_ref" ],
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta", checkIfExist: true)
+                    ] )
+
+                // ch_fai
+                input[5] = Channel.of(
+                    [
+                        [ id: "test_ref" ],
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai", checkIfExist: true)
+                    ] )
+
+                // ch rRNA interval list
+                input[6] = GATK4_BEDTOINTERVALLIST.out.interval_list
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/subworkflows/local/qc_workflow/test/nextflow.config b/subworkflows/local/qc_workflow/test/nextflow.config
new file mode 100644
index 000000000..498c456f0
--- /dev/null
+++ b/subworkflows/local/qc_workflow/test/nextflow.config
@@ -0,0 +1,10 @@
+process {
+    withName: PICARD_COLLECTRNASEQMETRICS {
+        ext.args = "--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND"
+    }
+
+    withName: GATK4_BEDTOINTERVALLIST {
+        ext.args = "--KEEP_LENGTH_ZERO_INTERVALS true"
+    }
+
+}

From 0a3f3e2d4052cad4ca08936a03676ca772d4a502 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Tue, 3 Dec 2024 19:56:54 +0000
Subject: [PATCH 130/887] update modules

---
 modules.json                            | 2 +-
 modules/nf-core/rrnatranscripts/main.nf | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules.json b/modules.json
index e3709392d..13b87c2e9 100644
--- a/modules.json
+++ b/modules.json
@@ -87,7 +87,7 @@
                     },
                     "rrnatranscripts": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "812edf8cf702de42d2d8c7314d6f03b97e20abeb",
                         "installed_by": ["modules"]
                     },
                     "salmon/index": {
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
index 50843c768..982c53cd4 100644
--- a/modules/nf-core/rrnatranscripts/main.nf
+++ b/modules/nf-core/rrnatranscripts/main.nf
@@ -1,5 +1,5 @@
 process RRNATRANSCRIPTS {
-    tag '$rrna'
+    tag "$gtf"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
@@ -20,7 +20,7 @@ process RRNATRANSCRIPTS {
     script:
     def prefix = task.ext.prefix ?: "${gtf.baseName}"
     """
-    grep -E '^#|rRNA' genome.gtf > ${prefix}_rrna_intervals.gtf || true
+    grep -E '^#|rRNA' ${gtf} > ${prefix}_rrna_intervals.gtf || true
     if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
         rm ${prefix}_rrna_intervals.gtf
     fi

From e63d5a9aff7d5ddaf8a0d5fbe51f38c66af87442 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Tue, 3 Dec 2024 19:57:16 +0000
Subject: [PATCH 131/887] run test and update workflow

---
 subworkflows/local/qc_workflow.nf             | 43 -----------
 .../local/qc_workflow/test/main.nf.test.snap  | 71 +++++++++++++++++++
 workflows/rnafusion.nf                        |  1 -
 3 files changed, 71 insertions(+), 44 deletions(-)
 delete mode 100644 subworkflows/local/qc_workflow.nf
 create mode 100644 subworkflows/local/qc_workflow/test/main.nf.test.snap

diff --git a/subworkflows/local/qc_workflow.nf b/subworkflows/local/qc_workflow.nf
deleted file mode 100644
index 5e121dc81..000000000
--- a/subworkflows/local/qc_workflow.nf
+++ /dev/null
@@ -1,43 +0,0 @@
-//
-// Check input samplesheet and get read channels
-//
-
-include { PICARD_COLLECTRNASEQMETRICS }                from '../../modules/nf-core/picard/collectrnaseqmetrics/main'
-include { GATK4_MARKDUPLICATES }                       from '../../modules/nf-core/gatk4/markduplicates/main'
-include { PICARD_COLLECTINSERTSIZEMETRICS }            from '../../modules/nf-core/picard/collectinsertsizemetrics/main'
-
-workflow QC_WORKFLOW {
-    take:
-        ch_reads_all
-        ch_bam_sorted
-        ch_chrgtf
-        ch_refflat
-        ch_fasta
-        ch_fai
-        ch_rrna_interval
-
-    main:
-        ch_versions = Channel.empty()
-
-        PICARD_COLLECTRNASEQMETRICS(ch_bam_sorted, ch_refflat.map { meta, refflat -> [refflat]},  ch_fasta.map { meta, fasta -> [fasta]}, ch_rrna_interval.map { meta, rrna_interval -> [rrna_interval]})
-        ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
-        ch_rnaseq_metrics = Channel.empty().mix(PICARD_COLLECTRNASEQMETRICS.out.metrics)
-
-        GATK4_MARKDUPLICATES(ch_bam_sorted, ch_fasta.map { meta, fasta -> [ fasta ]}, ch_fai.map { meta, fasta_fai -> [ fasta_fai ]})
-        ch_versions = ch_versions.mix(GATK4_MARKDUPLICATES.out.versions)
-        ch_duplicate_metrics = Channel.empty().mix(GATK4_MARKDUPLICATES.out.metrics)
-
-        PICARD_COLLECTINSERTSIZEMETRICS(ch_bam_sorted)
-        ch_versions = ch_versions.mix(PICARD_COLLECTINSERTSIZEMETRICS.out.versions)
-        ch_insertsize_metrics = Channel.empty().mix(PICARD_COLLECTINSERTSIZEMETRICS.out.metrics)
-
-
-
-    emit:
-        versions            = ch_versions
-        rnaseq_metrics      = ch_rnaseq_metrics
-        duplicate_metrics   = ch_duplicate_metrics
-        insertsize_metrics  = ch_insertsize_metrics
-
-}
-
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
new file mode 100644
index 000000000..3315ae19b
--- /dev/null
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -0,0 +1,71 @@
+{
+    "QC_WORKFLOW - Homo sapiens chr22": {
+        "content": [
+            {
+                "0": [
+                    "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139",
+                    "versions.yml:md5,5b495d995918c2b39708211c01036a5a",
+                    "versions.yml:md5,5c0ffe79bb7206c2652cf7f477f9b53f"
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "chr22_bam"
+                        },
+                        "chr22_bam.rna_metrics:md5,3415d05e545714d98114ab36a25afd91"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "chr22_bam"
+                        },
+                        "chr22_bam.bam.metrics:md5,3c9779078c3afdb9d438956473111bb5"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "chr22_bam"
+                        },
+                        "chr22_bam_collectinsertsize.txt:md5,846e2e72f7c4cb287d778b1739fa221e"
+                    ]
+                ],
+                "duplicate_metrics": [
+                    [
+                        {
+                            "id": "chr22_bam"
+                        },
+                        "chr22_bam.bam.metrics:md5,3c9779078c3afdb9d438956473111bb5"
+                    ]
+                ],
+                "insertsize_metrics": [
+                    [
+                        {
+                            "id": "chr22_bam"
+                        },
+                        "chr22_bam_collectinsertsize.txt:md5,846e2e72f7c4cb287d778b1739fa221e"
+                    ]
+                ],
+                "rnaseq_metrics": [
+                    [
+                        {
+                            "id": "chr22_bam"
+                        },
+                        "chr22_bam.rna_metrics:md5,3415d05e545714d98114ab36a25afd91"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139",
+                    "versions.yml:md5,5b495d995918c2b39708211c01036a5a",
+                    "versions.yml:md5,5c0ffe79bb7206c2652cf7f477f9b53f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-03T19:48:07.253685279"
+    }
+}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2a2c6d317..1f7890b54 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -147,7 +147,6 @@ workflow RNAFUSION {
 
     //QC
     QC_WORKFLOW (
-        ch_reads_all,
         STARFUSION_WORKFLOW.out.ch_bam_sorted,
         ch_chrgtf,
         ch_refflat,

From 474a89e9fec57aecacab55f1ee204f24dfd91389 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Tue, 3 Dec 2024 20:05:24 +0000
Subject: [PATCH 132/887] add missing conda lines

---
 modules/local/fusioninspector/main.nf     | 1 +
 modules/local/starfusion/build/main.nf    | 1 +
 modules/local/starfusion/detect/main.nf   | 1 +
 modules/local/starfusion/download/main.nf | 1 +
 4 files changed, 4 insertions(+)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 59d513fbb..ae05ceb42 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -2,6 +2,7 @@ process FUSIONINSPECTOR {
     tag "$meta.id"
     label 'process_high'
 
+    conda "${moduleDir}/environment.yml"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index cedf8c461..6ac3c6942 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -1,6 +1,7 @@
 process STARFUSION_BUILD {
     tag 'star-fusion'
 
+    conda "${moduleDir}/environment.yml"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 3ed54af2d..273967707 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -2,6 +2,7 @@ process STARFUSION {
     tag "$meta.id"
     label 'process_high'
 
+    conda "${moduleDir}/environment.yml"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     input:
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 250f13967..d06684869 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -1,6 +1,7 @@
 process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
+    conda "${moduleDir}/environment.yml"
     container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
 
     output:

From 7c942d88b98cbf9681283a66b0a2212b767cf6f8 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Tue, 3 Dec 2024 21:09:20 +0000
Subject: [PATCH 133/887] update test to constant poprtion

---
 .../local/qc_workflow/test/main.nf.test       |  26 +-
 .../local/qc_workflow/test/main.nf.test.snap  | 465 +++++++++++++++---
 2 files changed, 415 insertions(+), 76 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index 57dd38da5..66a4a2cb8 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -83,40 +83,32 @@ nextflow_workflow {
                         file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
                     ])
 
-                // ch_bam_sorted_indexed
-                input[1] = Channel.of(
-                    [
-                        [id: "chr22_bam"],
-                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true),
-                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai", checkIfExists: true)
-                    ])
-
                 // ch_chrgtf
-                input[2] = Channel.of(
+                input[1] = Channel.of(
                     [
                         [ id: "chr22_gtf" ],
                         file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
                     ])
 
                 // ch_refflat
-                input[3] = UCSC_GTFTOGENEPRED.out.refflat
+                input[2] = UCSC_GTFTOGENEPRED.out.refflat
 
                 // ch_fasta
-                input[4] = Channel.of(
+                input[3] = Channel.of(
                     [
                         [ id: "test_ref" ],
                         file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta", checkIfExist: true)
                     ] )
 
                 // ch_fai
-                input[5] = Channel.of(
+                input[4] = Channel.of(
                     [
                         [ id: "test_ref" ],
                         file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai", checkIfExist: true)
                     ] )
 
                 // ch rRNA interval list
-                input[6] = GATK4_BEDTOINTERVALLIST.out.interval_list
+                input[5] = GATK4_BEDTOINTERVALLIST.out.interval_list
                 """
             }
         }
@@ -124,10 +116,14 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { assert snapshot(workflow.out).match() }
+                { assert snapshot(
+                    file( workflow.out.versions[0] ),
+                    file( workflow.out.rnaseq_metrics[0][1] ).readLines()[4..-1],
+                    file( workflow.out.duplicate_metrics[0][1] ).readLines()[4..-1],
+                    file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1],
+                ).match() }
             )
         }
-
     }
 
 }
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index 3315ae19b..8fc12f11d 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -1,71 +1,414 @@
 {
     "QC_WORKFLOW - Homo sapiens chr22": {
         "content": [
-            {
-                "0": [
-                    "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139",
-                    "versions.yml:md5,5b495d995918c2b39708211c01036a5a",
-                    "versions.yml:md5,5c0ffe79bb7206c2652cf7f477f9b53f"
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "chr22_bam"
-                        },
-                        "chr22_bam.rna_metrics:md5,3415d05e545714d98114ab36a25afd91"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "chr22_bam"
-                        },
-                        "chr22_bam.bam.metrics:md5,3c9779078c3afdb9d438956473111bb5"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "chr22_bam"
-                        },
-                        "chr22_bam_collectinsertsize.txt:md5,846e2e72f7c4cb287d778b1739fa221e"
-                    ]
-                ],
-                "duplicate_metrics": [
-                    [
-                        {
-                            "id": "chr22_bam"
-                        },
-                        "chr22_bam.bam.metrics:md5,3c9779078c3afdb9d438956473111bb5"
-                    ]
-                ],
-                "insertsize_metrics": [
-                    [
-                        {
-                            "id": "chr22_bam"
-                        },
-                        "chr22_bam_collectinsertsize.txt:md5,846e2e72f7c4cb287d778b1739fa221e"
-                    ]
-                ],
-                "rnaseq_metrics": [
-                    [
-                        {
-                            "id": "chr22_bam"
-                        },
-                        "chr22_bam.rna_metrics:md5,3415d05e545714d98114ab36a25afd91"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139",
-                    "versions.yml:md5,5b495d995918c2b39708211c01036a5a",
-                    "versions.yml:md5,5c0ffe79bb7206c2652cf7f477f9b53f"
-                ]
-            }
+            "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139",
+            [
+                "",
+                "## METRICS CLASS\tpicard.analysis.RnaSeqMetrics",
+                "PF_BASES\tPF_ALIGNED_BASES\tRIBOSOMAL_BASES\tCODING_BASES\tUTR_BASES\tINTRONIC_BASES\tINTERGENIC_BASES\tIGNORED_READS\tCORRECT_STRAND_READS\tINCORRECT_STRAND_READS\tNUM_R1_TRANSCRIPT_STRAND_READS\tNUM_R2_TRANSCRIPT_STRAND_READS\tNUM_UNEXPLAINED_READS\tPCT_R1_TRANSCRIPT_STRAND_READS\tPCT_R2_TRANSCRIPT_STRAND_READS\tPCT_RIBOSOMAL_BASES\tPCT_CODING_BASES\tPCT_UTR_BASES\tPCT_INTRONIC_BASES\tPCT_INTERGENIC_BASES\tPCT_MRNA_BASES\tPCT_USABLE_BASES\tPCT_CORRECT_STRAND_READS\tMEDIAN_CV_COVERAGE\tMEDIAN_5PRIME_BIAS\tMEDIAN_3PRIME_BIAS\tMEDIAN_5PRIME_TO_3PRIME_BIAS\tSAMPLE\tLIBRARY\tREAD_GROUP",
+                "672131\t670989\t\t0\t323630\t84236\t263123\t0\t1324\t1328\t664\t661\t1\t0.501132\t0.498868\t\t0\t0.482318\t0.12554\t0.392142\t0.482318\t0.481498\t0.499246\t4.318717\t0\t0.529614\t0\t\t\t",
+                "",
+                "## HISTOGRAM\tjava.lang.Integer",
+                "normalized_position\tAll_Reads.normalized_coverage",
+                "0\t0",
+                "1\t0",
+                "2\t0",
+                "3\t0",
+                "4\t0",
+                "5\t0",
+                "6\t0",
+                "7\t0",
+                "8\t0",
+                "9\t0",
+                "10\t0",
+                "11\t0",
+                "12\t0",
+                "13\t0",
+                "14\t0",
+                "15\t0",
+                "16\t0",
+                "17\t0",
+                "18\t0",
+                "19\t0",
+                "20\t0",
+                "21\t0",
+                "22\t0",
+                "23\t0",
+                "24\t0",
+                "25\t0",
+                "26\t0",
+                "27\t0",
+                "28\t0",
+                "29\t0",
+                "30\t0",
+                "31\t0",
+                "32\t0",
+                "33\t0",
+                "34\t0",
+                "35\t0",
+                "36\t0",
+                "37\t0",
+                "38\t0",
+                "39\t0",
+                "40\t0",
+                "41\t0",
+                "42\t0",
+                "43\t0",
+                "44\t0",
+                "45\t0",
+                "46\t0",
+                "47\t0",
+                "48\t0",
+                "49\t0",
+                "50\t0",
+                "51\t0",
+                "52\t0",
+                "53\t0",
+                "54\t0",
+                "55\t0",
+                "56\t0",
+                "57\t0",
+                "58\t0",
+                "59\t0",
+                "60\t0",
+                "61\t0",
+                "62\t0.007542",
+                "63\t0.353257",
+                "64\t0.901997",
+                "65\t0.824165",
+                "66\t0.103172",
+                "67\t0.004348",
+                "68\t0",
+                "69\t0",
+                "70\t0",
+                "71\t0",
+                "72\t0",
+                "73\t0",
+                "74\t0",
+                "75\t0",
+                "76\t0",
+                "77\t0",
+                "78\t0",
+                "79\t0",
+                "80\t0",
+                "81\t0",
+                "82\t0",
+                "83\t0",
+                "84\t0",
+                "85\t0",
+                "86\t0",
+                "87\t0",
+                "88\t0",
+                "89\t0",
+                "90\t0",
+                "91\t0.651006",
+                "92\t6.035418",
+                "93\t15.914658",
+                "94\t23.771379",
+                "95\t25.867993",
+                "96\t18.602848",
+                "97\t6.578541",
+                "98\t0.156266",
+                "99\t0.03077",
+                "100\t0.012318",
+                ""
+            ],
+            [
+                "",
+                "## METRICS CLASS\tpicard.sam.DuplicationMetrics",
+                "LIBRARY\tUNPAIRED_READS_EXAMINED\tREAD_PAIRS_EXAMINED\tSECONDARY_OR_SUPPLEMENTARY_RDS\tUNMAPPED_READS\tUNPAIRED_READ_DUPLICATES\tREAD_PAIR_DUPLICATES\tREAD_PAIR_OPTICAL_DUPLICATES\tPERCENT_DUPLICATION\tESTIMATED_LIBRARY_SIZE",
+                "testN\t0\t2820\t2\t2\t0\t828\t0\t0.293617\t3807",
+                "",
+                "## HISTOGRAM\tjava.lang.Double",
+                "BIN\tCoverageMult\tall_sets\tnon_optical_sets",
+                "1.0\t0.999986\t1178\t1178",
+                "2.0\t1.47674\t800\t800",
+                "3.0\t1.704038\t14\t14",
+                "4.0\t1.812404\t0\t0",
+                "5.0\t1.864069\t0\t0",
+                "6.0\t1.888701\t0\t0",
+                "7.0\t1.900444\t0\t0",
+                "8.0\t1.906043\t0\t0",
+                "9.0\t1.908712\t0\t0",
+                "10.0\t1.909985\t0\t0",
+                "11.0\t1.910592\t0\t0",
+                "12.0\t1.910881\t0\t0",
+                "13.0\t1.911019\t0\t0",
+                "14.0\t1.911085\t0\t0",
+                "15.0\t1.911116\t0\t0",
+                "16.0\t1.911131\t0\t0",
+                "17.0\t1.911138\t0\t0",
+                "18.0\t1.911141\t0\t0",
+                "19.0\t1.911143\t0\t0",
+                "20.0\t1.911144\t0\t0",
+                "21.0\t1.911144\t0\t0",
+                "22.0\t1.911144\t0\t0",
+                "23.0\t1.911145\t0\t0",
+                "24.0\t1.911145\t0\t0",
+                "25.0\t1.911145\t0\t0",
+                "26.0\t1.911145\t0\t0",
+                "27.0\t1.911145\t0\t0",
+                "28.0\t1.911145\t0\t0",
+                "29.0\t1.911145\t0\t0",
+                "30.0\t1.911145\t0\t0",
+                "31.0\t1.911145\t0\t0",
+                "32.0\t1.911145\t0\t0",
+                "33.0\t1.911145\t0\t0",
+                "34.0\t1.911145\t0\t0",
+                "35.0\t1.911145\t0\t0",
+                "36.0\t1.911145\t0\t0",
+                "37.0\t1.911145\t0\t0",
+                "38.0\t1.911145\t0\t0",
+                "39.0\t1.911145\t0\t0",
+                "40.0\t1.911145\t0\t0",
+                "41.0\t1.911145\t0\t0",
+                "42.0\t1.911145\t0\t0",
+                "43.0\t1.911145\t0\t0",
+                "44.0\t1.911145\t0\t0",
+                "45.0\t1.911145\t0\t0",
+                "46.0\t1.911145\t0\t0",
+                "47.0\t1.911145\t0\t0",
+                "48.0\t1.911145\t0\t0",
+                "49.0\t1.911145\t0\t0",
+                "50.0\t1.911145\t0\t0",
+                "51.0\t1.911145\t0\t0",
+                "52.0\t1.911145\t0\t0",
+                "53.0\t1.911145\t0\t0",
+                "54.0\t1.911145\t0\t0",
+                "55.0\t1.911145\t0\t0",
+                "56.0\t1.911145\t0\t0",
+                "57.0\t1.911145\t0\t0",
+                "58.0\t1.911145\t0\t0",
+                "59.0\t1.911145\t0\t0",
+                "60.0\t1.911145\t0\t0",
+                "61.0\t1.911145\t0\t0",
+                "62.0\t1.911145\t0\t0",
+                "63.0\t1.911145\t0\t0",
+                "64.0\t1.911145\t0\t0",
+                "65.0\t1.911145\t0\t0",
+                "66.0\t1.911145\t0\t0",
+                "67.0\t1.911145\t0\t0",
+                "68.0\t1.911145\t0\t0",
+                "69.0\t1.911145\t0\t0",
+                "70.0\t1.911145\t0\t0",
+                "71.0\t1.911145\t0\t0",
+                "72.0\t1.911145\t0\t0",
+                "73.0\t1.911145\t0\t0",
+                "74.0\t1.911145\t0\t0",
+                "75.0\t1.911145\t0\t0",
+                "76.0\t1.911145\t0\t0",
+                "77.0\t1.911145\t0\t0",
+                "78.0\t1.911145\t0\t0",
+                "79.0\t1.911145\t0\t0",
+                "80.0\t1.911145\t0\t0",
+                "81.0\t1.911145\t0\t0",
+                "82.0\t1.911145\t0\t0",
+                "83.0\t1.911145\t0\t0",
+                "84.0\t1.911145\t0\t0",
+                "85.0\t1.911145\t0\t0",
+                "86.0\t1.911145\t0\t0",
+                "87.0\t1.911145\t0\t0",
+                "88.0\t1.911145\t0\t0",
+                "89.0\t1.911145\t0\t0",
+                "90.0\t1.911145\t0\t0",
+                "91.0\t1.911145\t0\t0",
+                "92.0\t1.911145\t0\t0",
+                "93.0\t1.911145\t0\t0",
+                "94.0\t1.911145\t0\t0",
+                "95.0\t1.911145\t0\t0",
+                "96.0\t1.911145\t0\t0",
+                "97.0\t1.911145\t0\t0",
+                "98.0\t1.911145\t0\t0",
+                "99.0\t1.911145\t0\t0",
+                "100.0\t1.911145\t0\t0",
+                ""
+            ],
+            [
+                "",
+                "## METRICS CLASS\tpicard.analysis.InsertSizeMetrics",
+                "MEDIAN_INSERT_SIZE\tMODE_INSERT_SIZE\tMEDIAN_ABSOLUTE_DEVIATION\tMIN_INSERT_SIZE\tMAX_INSERT_SIZE\tMEAN_INSERT_SIZE\tSTANDARD_DEVIATION\tREAD_PAIRS\tPAIR_ORIENTATION\tWIDTH_OF_10_PERCENT\tWIDTH_OF_20_PERCENT\tWIDTH_OF_30_PERCENT\tWIDTH_OF_40_PERCENT\tWIDTH_OF_50_PERCENT\tWIDTH_OF_60_PERCENT\tWIDTH_OF_70_PERCENT\tWIDTH_OF_80_PERCENT\tWIDTH_OF_90_PERCENT\tWIDTH_OF_95_PERCENT\tWIDTH_OF_99_PERCENT\tSAMPLE\tLIBRARY\tREAD_GROUP",
+                "123\t96\t23\t32\t300\t125.684397\t32.447863\t2820\tFR\t11\t17\t27\t37\t47\t57\t69\t83\t103\t125\t169\t\t\t",
+                "",
+                "## HISTOGRAM\tjava.lang.Integer",
+                "insert_size\tAll_Reads.fr_count",
+                "32\t1",
+                "41\t1",
+                "49\t3",
+                "51\t1",
+                "52\t2",
+                "54\t1",
+                "58\t1",
+                "59\t2",
+                "60\t1",
+                "61\t4",
+                "62\t1",
+                "63\t5",
+                "65\t5",
+                "66\t2",
+                "67\t6",
+                "68\t3",
+                "69\t5",
+                "70\t10",
+                "71\t11",
+                "72\t7",
+                "73\t8",
+                "74\t4",
+                "75\t12",
+                "76\t11",
+                "77\t19",
+                "78\t15",
+                "79\t13",
+                "80\t17",
+                "81\t24",
+                "82\t18",
+                "83\t19",
+                "84\t25",
+                "85\t15",
+                "86\t24",
+                "87\t30",
+                "88\t29",
+                "89\t21",
+                "90\t16",
+                "91\t24",
+                "92\t30",
+                "93\t23",
+                "94\t21",
+                "95\t43",
+                "96\t54",
+                "97\t34",
+                "98\t28",
+                "99\t24",
+                "100\t44",
+                "101\t24",
+                "102\t27",
+                "103\t22",
+                "104\t33",
+                "105\t26",
+                "106\t28",
+                "107\t35",
+                "108\t26",
+                "109\t24",
+                "110\t34",
+                "111\t29",
+                "112\t22",
+                "113\t36",
+                "114\t30",
+                "115\t49",
+                "116\t36",
+                "117\t33",
+                "118\t34",
+                "119\t38",
+                "120\t14",
+                "121\t39",
+                "122\t30",
+                "123\t28",
+                "124\t36",
+                "125\t36",
+                "126\t25",
+                "127\t32",
+                "128\t31",
+                "129\t28",
+                "130\t39",
+                "131\t45",
+                "132\t25",
+                "133\t18",
+                "134\t25",
+                "135\t31",
+                "136\t30",
+                "137\t29",
+                "138\t34",
+                "139\t32",
+                "140\t28",
+                "141\t41",
+                "142\t27",
+                "143\t23",
+                "144\t26",
+                "145\t31",
+                "146\t21",
+                "147\t29",
+                "148\t18",
+                "149\t17",
+                "150\t19",
+                "151\t20",
+                "152\t28",
+                "153\t28",
+                "154\t18",
+                "155\t23",
+                "156\t20",
+                "157\t29",
+                "158\t16",
+                "159\t15",
+                "160\t14",
+                "161\t18",
+                "162\t19",
+                "163\t15",
+                "164\t9",
+                "165\t11",
+                "166\t21",
+                "167\t9",
+                "168\t17",
+                "169\t16",
+                "170\t17",
+                "171\t13",
+                "172\t14",
+                "173\t21",
+                "174\t9",
+                "175\t9",
+                "176\t7",
+                "177\t9",
+                "178\t9",
+                "179\t9",
+                "180\t2",
+                "181\t8",
+                "182\t8",
+                "183\t3",
+                "184\t12",
+                "185\t10",
+                "186\t5",
+                "187\t7",
+                "188\t1",
+                "189\t5",
+                "190\t8",
+                "191\t10",
+                "192\t8",
+                "193\t2",
+                "194\t6",
+                "195\t1",
+                "196\t2",
+                "197\t3",
+                "198\t2",
+                "199\t4",
+                "200\t7",
+                "201\t2",
+                "202\t6",
+                "203\t4",
+                "204\t4",
+                "205\t2",
+                "206\t4",
+                "207\t4",
+                "209\t1",
+                "210\t1",
+                "212\t2",
+                "213\t4",
+                "214\t3",
+                "215\t1",
+                "216\t4",
+                "218\t2",
+                "220\t1",
+                "221\t2",
+                "223\t1",
+                "224\t1",
+                "231\t1",
+                "236\t1",
+                "239\t1",
+                "300\t1",
+                ""
+            ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T19:48:07.253685279"
+        "timestamp": "2024-12-03T21:08:20.661591983"
     }
 }
\ No newline at end of file

From 4d08dfdac5fb01a7c6619c46b2b15474165312d4 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Wed, 4 Dec 2024 00:37:23 +0000
Subject: [PATCH 134/887] update changelog

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a1a68d62d..8ffbebd18 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to nf-core module: `PICARD_COLLECTRNASEQMETRICS` and update module [#551](https://github.com/nf-core/rnafusion/pull/551)
 - Add `--skip_vcf` boolean parameter to skip vcf file generation [#554](https://github.com/nf-core/rnafusion/pull/554)
 - Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
+- Add nf-test to local subworkflow: `QC_SUBWORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 
 ### Changed
 
@@ -24,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
+- Remove unused input channel from `QC_SUBWORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 
 ### Fixed
 

From 13588e4a79260ac0a9c59b1f04bfdf6d4c421cc6 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?=
 <64787947+alanmmobbs93@users.noreply.github.com>
Date: Wed, 4 Dec 2024 08:47:53 -0300
Subject: [PATCH 135/887] Remove line from changelog

---
 CHANGELOG.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8ffbebd18..11444abe0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -25,7 +25,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
-- Remove unused input channel from `QC_SUBWORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 
 ### Fixed
 

From be02390bbefa0aa7ab7fde26e672a2ff57957b62 Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Wed, 4 Dec 2024 14:46:33 +0000
Subject: [PATCH 136/887] Update snaps and profile resources

---
 conf/test.config              | 8 --------
 conf/test_build.config        | 8 --------
 conf/test_cosmic.config       | 8 --------
 nf-test.config                | 5 +----
 tests/nextflow.config         | 8 ++++++++
 tests/test_build.nf.test.snap | 8 ++++----
 6 files changed, 13 insertions(+), 32 deletions(-)
 create mode 100644 tests/nextflow.config

diff --git a/conf/test.config b/conf/test.config
index aa51522d7..3cafa252b 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -10,14 +10,6 @@
 ----------------------------------------------------------------------------------------
 */
 
-process {
-    resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
-        time: '1.h'
-    ]
-}
-
 params {
     config_profile_name        = 'Test profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_build.config b/conf/test_build.config
index 02ca2a170..b335963dc 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -10,14 +10,6 @@
 ----------------------------------------------------------------------------------------
 */
 
-process {
-    resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
-        time: '1.h'
-    ]
-}
-
 params {
     config_profile_name        = 'Test build references profile'
     config_profile_description = 'Minimal test dataset to check pipeline function'
diff --git a/conf/test_cosmic.config b/conf/test_cosmic.config
index ebbad7cf3..cf199da4d 100644
--- a/conf/test_cosmic.config
+++ b/conf/test_cosmic.config
@@ -10,14 +10,6 @@
 ----------------------------------------------------------------------------------------
 */
 
-process {
-    resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
-        time: '1.h'
-    ]
-}
-
 params {
     config_profile_name        = 'Test cosmic profile'
     config_profile_description = 'Minimal test cosmic dataset to check pipeline function'
diff --git a/nf-test.config b/nf-test.config
index 16f182d49..3ee9cc134 100644
--- a/nf-test.config
+++ b/nf-test.config
@@ -5,11 +5,8 @@ config {
     // nf-test directory including temporary files for each test
     workDir ".nf-test"
 
-    // location of library folder that is added automatically to the classpath
-    libDir "tests/lib/"
-
     // location of an optional nextflow.config file specific for executing tests
-    configFile "nextflow.config"
+    configFile "tests/nextflow.config"
 
     plugins {
         load "nft-utils@0.0.3"
diff --git a/tests/nextflow.config b/tests/nextflow.config
new file mode 100644
index 000000000..c3f050dcb
--- /dev/null
+++ b/tests/nextflow.config
@@ -0,0 +1,8 @@
+// Limit and standardize resources for github actions and reproducibility
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 0833ced64..bcd299593 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -39,9 +39,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2024-12-03T19:26:00.897559313"
+        "timestamp": "2024-12-04T15:38:32.063684"
     }
-}
\ No newline at end of file
+}

From 9683f58e3eea58466dc26c8a92c1f0e93744c29e Mon Sep 17 00:00:00 2001
From: nschcolnicov <nico.schcolnicov@gmail.com>
Date: Wed, 4 Dec 2024 17:59:00 +0000
Subject: [PATCH 137/887] Add salmon index skip parameter

---
 conf/test_build.config        |  2 ++
 nextflow.config               |  1 +
 nextflow_schema.json          |  5 +++++
 tests/test_build.nf.test.snap | 12 ++++++------
 workflows/build_references.nf |  5 ++++-
 5 files changed, 18 insertions(+), 7 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index b335963dc..616d734f0 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -19,4 +19,6 @@ params {
     input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
     no_cosmic                  = true
     all                        = true
+
+    skip_salmon_index          = true
 }
diff --git a/nextflow.config b/nextflow.config
index f2441505f..d436182c4 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -72,6 +72,7 @@ params {
     skip_qc                    = false
     skip_vis                   = false
     skip_vcf                   = false
+    skip_salmon_index          = false
 
     // Path to references
     no_cosmic                     = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index e5d3866dd..017a19fb8 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -22,6 +22,11 @@
                 "skip_vcf": {
                     "type": "boolean",
                     "description": "Skip vcf generation step"
+                },
+                "skip_salmon_index": {
+                    "type": "boolean",
+                    "description": "Skip salmon index generation step",
+                    "hidden": true
                 }
             },
             "fa_icon": "fas fa-fast-forward"
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index bcd299593..3b5441dc1 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -7,7 +7,7 @@
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
                 "gffread",
-                "gffread/Homo_sapiens.GRCh38.102.gff3",
+                "gffread/Homo_sapiens.GRCh38.102.fasta",
                 "pipeline_info",
                 "references",
                 "references/ensembl",
@@ -27,7 +27,7 @@
             [
                 "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,5bc3ccc76735ae46699a75269f0ea65b",
                 "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,092f1bf29fce906ff7b5ece02b4b21c8",
-                "Homo_sapiens.GRCh38.102.gff3:md5,f559ec7a1e361351b97f4856ddfcbf84",
+                "Homo_sapiens.GRCh38.102.fasta:md5,d1f17b045dc60c49f2cc29e30006afc0",
                 "Homo_sapiens.GRCh38.102.chr.gtf:md5,0069687307852c63596b4d2ebdbbaf0c",
                 "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d527f3eb6b664020cf4d882b5820056f",
                 "Homo_sapiens.GRCh38.102.genepred:md5,59c577ca4ab033c0ce1feac1e387bcab",
@@ -39,9 +39,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-04T15:38:32.063684"
+        "timestamp": "2024-12-04T17:55:45.068780109"
     }
-}
+}
\ No newline at end of file
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index def3c1627..4886a7bb7 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -49,7 +49,10 @@ workflow BUILD_REFERENCES {
     GATK4_BEDTOINTERVALLIST(BEDOPS_CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
 
     GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ENSEMBL_DOWNLOAD.out.primary_assembly.map { meta, fasta -> [ fasta ] })
-    SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+
+    if (!params.skip_salmon_index){
+        SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+    }
 
     if (params.starindex || params.all || params.starfusion || params.arriba) {
         STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )

From a8f9bc4486c7cfb53f818588cef36f5521f7e42b Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Wed, 4 Dec 2024 19:17:33 +0000
Subject: [PATCH 138/887] add md5 to snaps

---
 .../local/qc_workflow/test/main.nf.test       |  16 +-
 .../local/qc_workflow/test/main.nf.test.snap  | 432 ++----------------
 2 files changed, 37 insertions(+), 411 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index 66a4a2cb8..8929291ec 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -116,12 +116,16 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { assert snapshot(
-                    file( workflow.out.versions[0] ),
-                    file( workflow.out.rnaseq_metrics[0][1] ).readLines()[4..-1],
-                    file( workflow.out.duplicate_metrics[0][1] ).readLines()[4..-1],
-                    file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1],
-                ).match() }
+                { assert snapshot(file( workflow.out.versions[0] )).match('versions' )                                                },
+                { assert snapshot(file( workflow.out.rnaseq_metrics[0][1]     ).readLines()[4..-1]).md5().match('rnaseq_metrics'    ) },
+                { assert snapshot(file( workflow.out.duplicate_metrics[0][1]  ).readLines()[4..-1]).md5().match('duplicate_metrics' ) },
+                { assert snapshot(file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1]).md5().match('insertsize_metrics') }
+
+                    //file( workflow.out.versions[0] ),
+                    //file( workflow.out.rnaseq_metrics[0][1] ).readLines()[4..-1],
+                    //file( workflow.out.duplicate_metrics[0][1] ).readLines()[4..-1],
+                    //file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1],
+                //).md5().match() }
             )
         }
     }
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index 8fc12f11d..c9b98e8ee 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -1,414 +1,36 @@
 {
-    "QC_WORKFLOW - Homo sapiens chr22": {
+    "insertsize_metric": {
+        "content": "160db81b19843c4d46fe74ac61f9f013",
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-04T19:10:04.844976546"
+    },
+    "versions": {
         "content": [
-            "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139",
-            [
-                "",
-                "## METRICS CLASS\tpicard.analysis.RnaSeqMetrics",
-                "PF_BASES\tPF_ALIGNED_BASES\tRIBOSOMAL_BASES\tCODING_BASES\tUTR_BASES\tINTRONIC_BASES\tINTERGENIC_BASES\tIGNORED_READS\tCORRECT_STRAND_READS\tINCORRECT_STRAND_READS\tNUM_R1_TRANSCRIPT_STRAND_READS\tNUM_R2_TRANSCRIPT_STRAND_READS\tNUM_UNEXPLAINED_READS\tPCT_R1_TRANSCRIPT_STRAND_READS\tPCT_R2_TRANSCRIPT_STRAND_READS\tPCT_RIBOSOMAL_BASES\tPCT_CODING_BASES\tPCT_UTR_BASES\tPCT_INTRONIC_BASES\tPCT_INTERGENIC_BASES\tPCT_MRNA_BASES\tPCT_USABLE_BASES\tPCT_CORRECT_STRAND_READS\tMEDIAN_CV_COVERAGE\tMEDIAN_5PRIME_BIAS\tMEDIAN_3PRIME_BIAS\tMEDIAN_5PRIME_TO_3PRIME_BIAS\tSAMPLE\tLIBRARY\tREAD_GROUP",
-                "672131\t670989\t\t0\t323630\t84236\t263123\t0\t1324\t1328\t664\t661\t1\t0.501132\t0.498868\t\t0\t0.482318\t0.12554\t0.392142\t0.482318\t0.481498\t0.499246\t4.318717\t0\t0.529614\t0\t\t\t",
-                "",
-                "## HISTOGRAM\tjava.lang.Integer",
-                "normalized_position\tAll_Reads.normalized_coverage",
-                "0\t0",
-                "1\t0",
-                "2\t0",
-                "3\t0",
-                "4\t0",
-                "5\t0",
-                "6\t0",
-                "7\t0",
-                "8\t0",
-                "9\t0",
-                "10\t0",
-                "11\t0",
-                "12\t0",
-                "13\t0",
-                "14\t0",
-                "15\t0",
-                "16\t0",
-                "17\t0",
-                "18\t0",
-                "19\t0",
-                "20\t0",
-                "21\t0",
-                "22\t0",
-                "23\t0",
-                "24\t0",
-                "25\t0",
-                "26\t0",
-                "27\t0",
-                "28\t0",
-                "29\t0",
-                "30\t0",
-                "31\t0",
-                "32\t0",
-                "33\t0",
-                "34\t0",
-                "35\t0",
-                "36\t0",
-                "37\t0",
-                "38\t0",
-                "39\t0",
-                "40\t0",
-                "41\t0",
-                "42\t0",
-                "43\t0",
-                "44\t0",
-                "45\t0",
-                "46\t0",
-                "47\t0",
-                "48\t0",
-                "49\t0",
-                "50\t0",
-                "51\t0",
-                "52\t0",
-                "53\t0",
-                "54\t0",
-                "55\t0",
-                "56\t0",
-                "57\t0",
-                "58\t0",
-                "59\t0",
-                "60\t0",
-                "61\t0",
-                "62\t0.007542",
-                "63\t0.353257",
-                "64\t0.901997",
-                "65\t0.824165",
-                "66\t0.103172",
-                "67\t0.004348",
-                "68\t0",
-                "69\t0",
-                "70\t0",
-                "71\t0",
-                "72\t0",
-                "73\t0",
-                "74\t0",
-                "75\t0",
-                "76\t0",
-                "77\t0",
-                "78\t0",
-                "79\t0",
-                "80\t0",
-                "81\t0",
-                "82\t0",
-                "83\t0",
-                "84\t0",
-                "85\t0",
-                "86\t0",
-                "87\t0",
-                "88\t0",
-                "89\t0",
-                "90\t0",
-                "91\t0.651006",
-                "92\t6.035418",
-                "93\t15.914658",
-                "94\t23.771379",
-                "95\t25.867993",
-                "96\t18.602848",
-                "97\t6.578541",
-                "98\t0.156266",
-                "99\t0.03077",
-                "100\t0.012318",
-                ""
-            ],
-            [
-                "",
-                "## METRICS CLASS\tpicard.sam.DuplicationMetrics",
-                "LIBRARY\tUNPAIRED_READS_EXAMINED\tREAD_PAIRS_EXAMINED\tSECONDARY_OR_SUPPLEMENTARY_RDS\tUNMAPPED_READS\tUNPAIRED_READ_DUPLICATES\tREAD_PAIR_DUPLICATES\tREAD_PAIR_OPTICAL_DUPLICATES\tPERCENT_DUPLICATION\tESTIMATED_LIBRARY_SIZE",
-                "testN\t0\t2820\t2\t2\t0\t828\t0\t0.293617\t3807",
-                "",
-                "## HISTOGRAM\tjava.lang.Double",
-                "BIN\tCoverageMult\tall_sets\tnon_optical_sets",
-                "1.0\t0.999986\t1178\t1178",
-                "2.0\t1.47674\t800\t800",
-                "3.0\t1.704038\t14\t14",
-                "4.0\t1.812404\t0\t0",
-                "5.0\t1.864069\t0\t0",
-                "6.0\t1.888701\t0\t0",
-                "7.0\t1.900444\t0\t0",
-                "8.0\t1.906043\t0\t0",
-                "9.0\t1.908712\t0\t0",
-                "10.0\t1.909985\t0\t0",
-                "11.0\t1.910592\t0\t0",
-                "12.0\t1.910881\t0\t0",
-                "13.0\t1.911019\t0\t0",
-                "14.0\t1.911085\t0\t0",
-                "15.0\t1.911116\t0\t0",
-                "16.0\t1.911131\t0\t0",
-                "17.0\t1.911138\t0\t0",
-                "18.0\t1.911141\t0\t0",
-                "19.0\t1.911143\t0\t0",
-                "20.0\t1.911144\t0\t0",
-                "21.0\t1.911144\t0\t0",
-                "22.0\t1.911144\t0\t0",
-                "23.0\t1.911145\t0\t0",
-                "24.0\t1.911145\t0\t0",
-                "25.0\t1.911145\t0\t0",
-                "26.0\t1.911145\t0\t0",
-                "27.0\t1.911145\t0\t0",
-                "28.0\t1.911145\t0\t0",
-                "29.0\t1.911145\t0\t0",
-                "30.0\t1.911145\t0\t0",
-                "31.0\t1.911145\t0\t0",
-                "32.0\t1.911145\t0\t0",
-                "33.0\t1.911145\t0\t0",
-                "34.0\t1.911145\t0\t0",
-                "35.0\t1.911145\t0\t0",
-                "36.0\t1.911145\t0\t0",
-                "37.0\t1.911145\t0\t0",
-                "38.0\t1.911145\t0\t0",
-                "39.0\t1.911145\t0\t0",
-                "40.0\t1.911145\t0\t0",
-                "41.0\t1.911145\t0\t0",
-                "42.0\t1.911145\t0\t0",
-                "43.0\t1.911145\t0\t0",
-                "44.0\t1.911145\t0\t0",
-                "45.0\t1.911145\t0\t0",
-                "46.0\t1.911145\t0\t0",
-                "47.0\t1.911145\t0\t0",
-                "48.0\t1.911145\t0\t0",
-                "49.0\t1.911145\t0\t0",
-                "50.0\t1.911145\t0\t0",
-                "51.0\t1.911145\t0\t0",
-                "52.0\t1.911145\t0\t0",
-                "53.0\t1.911145\t0\t0",
-                "54.0\t1.911145\t0\t0",
-                "55.0\t1.911145\t0\t0",
-                "56.0\t1.911145\t0\t0",
-                "57.0\t1.911145\t0\t0",
-                "58.0\t1.911145\t0\t0",
-                "59.0\t1.911145\t0\t0",
-                "60.0\t1.911145\t0\t0",
-                "61.0\t1.911145\t0\t0",
-                "62.0\t1.911145\t0\t0",
-                "63.0\t1.911145\t0\t0",
-                "64.0\t1.911145\t0\t0",
-                "65.0\t1.911145\t0\t0",
-                "66.0\t1.911145\t0\t0",
-                "67.0\t1.911145\t0\t0",
-                "68.0\t1.911145\t0\t0",
-                "69.0\t1.911145\t0\t0",
-                "70.0\t1.911145\t0\t0",
-                "71.0\t1.911145\t0\t0",
-                "72.0\t1.911145\t0\t0",
-                "73.0\t1.911145\t0\t0",
-                "74.0\t1.911145\t0\t0",
-                "75.0\t1.911145\t0\t0",
-                "76.0\t1.911145\t0\t0",
-                "77.0\t1.911145\t0\t0",
-                "78.0\t1.911145\t0\t0",
-                "79.0\t1.911145\t0\t0",
-                "80.0\t1.911145\t0\t0",
-                "81.0\t1.911145\t0\t0",
-                "82.0\t1.911145\t0\t0",
-                "83.0\t1.911145\t0\t0",
-                "84.0\t1.911145\t0\t0",
-                "85.0\t1.911145\t0\t0",
-                "86.0\t1.911145\t0\t0",
-                "87.0\t1.911145\t0\t0",
-                "88.0\t1.911145\t0\t0",
-                "89.0\t1.911145\t0\t0",
-                "90.0\t1.911145\t0\t0",
-                "91.0\t1.911145\t0\t0",
-                "92.0\t1.911145\t0\t0",
-                "93.0\t1.911145\t0\t0",
-                "94.0\t1.911145\t0\t0",
-                "95.0\t1.911145\t0\t0",
-                "96.0\t1.911145\t0\t0",
-                "97.0\t1.911145\t0\t0",
-                "98.0\t1.911145\t0\t0",
-                "99.0\t1.911145\t0\t0",
-                "100.0\t1.911145\t0\t0",
-                ""
-            ],
-            [
-                "",
-                "## METRICS CLASS\tpicard.analysis.InsertSizeMetrics",
-                "MEDIAN_INSERT_SIZE\tMODE_INSERT_SIZE\tMEDIAN_ABSOLUTE_DEVIATION\tMIN_INSERT_SIZE\tMAX_INSERT_SIZE\tMEAN_INSERT_SIZE\tSTANDARD_DEVIATION\tREAD_PAIRS\tPAIR_ORIENTATION\tWIDTH_OF_10_PERCENT\tWIDTH_OF_20_PERCENT\tWIDTH_OF_30_PERCENT\tWIDTH_OF_40_PERCENT\tWIDTH_OF_50_PERCENT\tWIDTH_OF_60_PERCENT\tWIDTH_OF_70_PERCENT\tWIDTH_OF_80_PERCENT\tWIDTH_OF_90_PERCENT\tWIDTH_OF_95_PERCENT\tWIDTH_OF_99_PERCENT\tSAMPLE\tLIBRARY\tREAD_GROUP",
-                "123\t96\t23\t32\t300\t125.684397\t32.447863\t2820\tFR\t11\t17\t27\t37\t47\t57\t69\t83\t103\t125\t169\t\t\t",
-                "",
-                "## HISTOGRAM\tjava.lang.Integer",
-                "insert_size\tAll_Reads.fr_count",
-                "32\t1",
-                "41\t1",
-                "49\t3",
-                "51\t1",
-                "52\t2",
-                "54\t1",
-                "58\t1",
-                "59\t2",
-                "60\t1",
-                "61\t4",
-                "62\t1",
-                "63\t5",
-                "65\t5",
-                "66\t2",
-                "67\t6",
-                "68\t3",
-                "69\t5",
-                "70\t10",
-                "71\t11",
-                "72\t7",
-                "73\t8",
-                "74\t4",
-                "75\t12",
-                "76\t11",
-                "77\t19",
-                "78\t15",
-                "79\t13",
-                "80\t17",
-                "81\t24",
-                "82\t18",
-                "83\t19",
-                "84\t25",
-                "85\t15",
-                "86\t24",
-                "87\t30",
-                "88\t29",
-                "89\t21",
-                "90\t16",
-                "91\t24",
-                "92\t30",
-                "93\t23",
-                "94\t21",
-                "95\t43",
-                "96\t54",
-                "97\t34",
-                "98\t28",
-                "99\t24",
-                "100\t44",
-                "101\t24",
-                "102\t27",
-                "103\t22",
-                "104\t33",
-                "105\t26",
-                "106\t28",
-                "107\t35",
-                "108\t26",
-                "109\t24",
-                "110\t34",
-                "111\t29",
-                "112\t22",
-                "113\t36",
-                "114\t30",
-                "115\t49",
-                "116\t36",
-                "117\t33",
-                "118\t34",
-                "119\t38",
-                "120\t14",
-                "121\t39",
-                "122\t30",
-                "123\t28",
-                "124\t36",
-                "125\t36",
-                "126\t25",
-                "127\t32",
-                "128\t31",
-                "129\t28",
-                "130\t39",
-                "131\t45",
-                "132\t25",
-                "133\t18",
-                "134\t25",
-                "135\t31",
-                "136\t30",
-                "137\t29",
-                "138\t34",
-                "139\t32",
-                "140\t28",
-                "141\t41",
-                "142\t27",
-                "143\t23",
-                "144\t26",
-                "145\t31",
-                "146\t21",
-                "147\t29",
-                "148\t18",
-                "149\t17",
-                "150\t19",
-                "151\t20",
-                "152\t28",
-                "153\t28",
-                "154\t18",
-                "155\t23",
-                "156\t20",
-                "157\t29",
-                "158\t16",
-                "159\t15",
-                "160\t14",
-                "161\t18",
-                "162\t19",
-                "163\t15",
-                "164\t9",
-                "165\t11",
-                "166\t21",
-                "167\t9",
-                "168\t17",
-                "169\t16",
-                "170\t17",
-                "171\t13",
-                "172\t14",
-                "173\t21",
-                "174\t9",
-                "175\t9",
-                "176\t7",
-                "177\t9",
-                "178\t9",
-                "179\t9",
-                "180\t2",
-                "181\t8",
-                "182\t8",
-                "183\t3",
-                "184\t12",
-                "185\t10",
-                "186\t5",
-                "187\t7",
-                "188\t1",
-                "189\t5",
-                "190\t8",
-                "191\t10",
-                "192\t8",
-                "193\t2",
-                "194\t6",
-                "195\t1",
-                "196\t2",
-                "197\t3",
-                "198\t2",
-                "199\t4",
-                "200\t7",
-                "201\t2",
-                "202\t6",
-                "203\t4",
-                "204\t4",
-                "205\t2",
-                "206\t4",
-                "207\t4",
-                "209\t1",
-                "210\t1",
-                "212\t2",
-                "213\t4",
-                "214\t3",
-                "215\t1",
-                "216\t4",
-                "218\t2",
-                "220\t1",
-                "221\t2",
-                "223\t1",
-                "224\t1",
-                "231\t1",
-                "236\t1",
-                "239\t1",
-                "300\t1",
-                ""
-            ]
+            "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139"
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T21:08:20.661591983"
+        "timestamp": "2024-12-04T19:10:04.80151539"
+    },
+    "duplicate_metric": {
+        "content": "651d8a4702f9f9871e94afbce3e50e34",
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-04T19:10:04.837403415"
+    },
+    "rnaseq_metric": {
+        "content": "84a348c3735ed2f6c47f346eeed661f4",
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-04T19:10:04.828498524"
     }
 }
\ No newline at end of file

From 40e30a3e8e1e67e243e055bcfa0abc7cb3d2d1ec Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Wed, 4 Dec 2024 19:21:37 +0000
Subject: [PATCH 139/887] Update comments on the subworkflow

---
 subworkflows/local/qc_workflow/main.nf | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/subworkflows/local/qc_workflow/main.nf b/subworkflows/local/qc_workflow/main.nf
index a8473d446..a0ad60b64 100644
--- a/subworkflows/local/qc_workflow/main.nf
+++ b/subworkflows/local/qc_workflow/main.nf
@@ -1,5 +1,5 @@
 //
-// Check input samplesheet and get read channels
+// Extract descriptive values from BAMs
 //
 
 include { PICARD_COLLECTRNASEQMETRICS       } from '../../../modules/nf-core/picard/collectrnaseqmetrics'
@@ -31,10 +31,10 @@ workflow QC_WORKFLOW {
         ch_insertsize_metrics = PICARD_COLLECTINSERTSIZEMETRICS.out.metrics
 
     emit:
-        versions            = ch_versions
-        rnaseq_metrics      = ch_rnaseq_metrics
-        duplicate_metrics   = ch_duplicate_metrics
-        insertsize_metrics  = ch_insertsize_metrics
+        versions            = ch_versions            // channel [ path       ]
+        rnaseq_metrics      = ch_rnaseq_metrics      // channel [ meta, path ]
+        duplicate_metrics   = ch_duplicate_metrics   // channel [ meta, path ]
+        insertsize_metrics  = ch_insertsize_metrics  // channel [ meta, path ]
 
 }
 

From 3c630e901b46184ea273e17c03cdac5b68482c00 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Wed, 4 Dec 2024 19:23:00 +0000
Subject: [PATCH 140/887] remove commented lines

---
 subworkflows/local/qc_workflow/test/main.nf.test | 6 ------
 1 file changed, 6 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index 8929291ec..cfb48e06d 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -120,12 +120,6 @@ nextflow_workflow {
                 { assert snapshot(file( workflow.out.rnaseq_metrics[0][1]     ).readLines()[4..-1]).md5().match('rnaseq_metrics'    ) },
                 { assert snapshot(file( workflow.out.duplicate_metrics[0][1]  ).readLines()[4..-1]).md5().match('duplicate_metrics' ) },
                 { assert snapshot(file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1]).md5().match('insertsize_metrics') }
-
-                    //file( workflow.out.versions[0] ),
-                    //file( workflow.out.rnaseq_metrics[0][1] ).readLines()[4..-1],
-                    //file( workflow.out.duplicate_metrics[0][1] ).readLines()[4..-1],
-                    //file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1],
-                //).md5().match() }
             )
         }
     }

From c8247c8238865d5ed54836360555a19c4df9f790 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Wed, 4 Dec 2024 19:33:30 +0000
Subject: [PATCH 141/887] update test snaps

---
 .../local/qc_workflow/test/main.nf.test.snap  | 20 +++++++++----------
 1 file changed, 10 insertions(+), 10 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index c9b98e8ee..b56c12b98 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -1,11 +1,11 @@
 {
-    "insertsize_metric": {
-        "content": "160db81b19843c4d46fe74ac61f9f013",
+    "duplicate_metrics": {
+        "content": "651d8a4702f9f9871e94afbce3e50e34",
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-04T19:10:04.844976546"
+        "timestamp": "2024-12-04T19:29:09.711230835"
     },
     "versions": {
         "content": [
@@ -15,22 +15,22 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-04T19:10:04.80151539"
+        "timestamp": "2024-12-04T19:29:09.686297468"
     },
-    "duplicate_metric": {
-        "content": "651d8a4702f9f9871e94afbce3e50e34",
+    "rnaseq_metrics": {
+        "content": "84a348c3735ed2f6c47f346eeed661f4",
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-04T19:10:04.837403415"
+        "timestamp": "2024-12-04T19:29:09.704632753"
     },
-    "rnaseq_metric": {
-        "content": "84a348c3735ed2f6c47f346eeed661f4",
+    "insertsize_metrics": {
+        "content": "160db81b19843c4d46fe74ac61f9f013",
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-04T19:10:04.828498524"
+        "timestamp": "2024-12-04T19:29:09.717929716"
     }
 }
\ No newline at end of file

From d8bfba2e0d165d0340034d1bb8b68744b40e1f71 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 13:45:32 +0000
Subject: [PATCH 142/887] change module structure and test

---
 subworkflows/local/trim_subworkflow/main.nf   | 46 +++++++++++++++++
 .../local/trim_subworkflow/tests/main.nf.test | 50 +++++++++++++++++++
 subworkflows/local/trim_workflow.nf           | 42 ----------------
 workflows/rnafusion.nf                        | 41 ++++++++-------
 4 files changed, 119 insertions(+), 60 deletions(-)
 create mode 100644 subworkflows/local/trim_subworkflow/main.nf
 create mode 100644 subworkflows/local/trim_subworkflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/trim_workflow.nf

diff --git a/subworkflows/local/trim_subworkflow/main.nf b/subworkflows/local/trim_subworkflow/main.nf
new file mode 100644
index 000000000..2cbb8380d
--- /dev/null
+++ b/subworkflows/local/trim_subworkflow/main.nf
@@ -0,0 +1,46 @@
+
+
+include { FASTP                      } from '../../../modules/nf-core/fastp/main'
+include { FASTQC as FASTQC_FOR_FASTP } from '../../../modules/nf-core/fastqc/main'
+
+workflow TRIM_WORKFLOW {
+
+    take:
+        reads           // channel [ meta, [ fastqs ] ]
+        adapter_fasta   // channel [ path ]
+        fastp_trim      // boolean
+
+    main:
+        ch_versions       = Channel.empty()
+        ch_fastp_html     = Channel.empty()
+        ch_fastp_json     = Channel.empty()
+        ch_fastqc_trimmed = Channel.empty()
+
+        if ( fastp_trim ) {
+            FASTP(reads, adapter_fasta.ifEmpty( [] ), false, false, false)
+            ch_versions = ch_versions.mix(FASTP.out.versions)
+
+            FASTQC_FOR_FASTP(FASTP.out.reads)
+            ch_versions = ch_versions.mix(FASTQC_FOR_FASTP.out.versions)
+
+            ch_reads_all           = FASTP.out.reads
+            ch_reads_fusioncatcher = ch_reads_all
+            ch_fastp_html          = FASTP.out.html
+            ch_fastp_json          = FASTP.out.json
+            ch_fastqc_trimmed      = FASTQC_FOR_FASTP.out.zip
+
+        }
+        else {
+            ch_reads_all           = reads
+            ch_reads_fusioncatcher = reads
+        }
+
+    emit:
+        ch_reads_all            // Channel [ meta, [reads]   ]
+        ch_reads_fusioncatcher  // Channel [ meta, [reads]   ]
+        ch_fastp_html           // Channel [ meta, path_html ]
+        ch_fastp_json           // Channel [ meta, path_json ]
+        ch_fastqc_trimmed       // Channel [ meta, path_zip  ]
+        versions = ch_versions  // Channel [ versions        ]
+    }
+
diff --git a/subworkflows/local/trim_subworkflow/tests/main.nf.test b/subworkflows/local/trim_subworkflow/tests/main.nf.test
new file mode 100644
index 000000000..66007f0d4
--- /dev/null
+++ b/subworkflows/local/trim_subworkflow/tests/main.nf.test
@@ -0,0 +1,50 @@
+nextflow_workflow {
+
+    name "Test Subworkflow QC_WORKFLOW"
+    script "../main.nf"
+    //config "./nextflow.config"
+    workflow "TRIM_WORKFLOW"
+    tag "qc"
+    tag "subworkflow"
+
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP true") {
+
+        when {
+            params {
+                fastp_trim = true
+            }
+
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [id: "test_fastq"],
+                        [
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
+                        ]
+                    ])
+                input[1] = Channel.empty()
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { with(workflow.out) {
+                    assert snapshot(
+                        versions.collect{ file(it) },
+                        ch_reads_all[0][1].collect { file(it) },
+                        ch_reads_fusioncatcher[0][1].collect { file(it) },
+                        file(ch_fastp_html[0][1]).name,
+                        file(ch_fastp_json[0][1]),
+                        ch_fastqc_trimmed[0][1].collect { file(it).name }
+                    ).match() }
+                }
+            )
+        }
+    }
+}
diff --git a/subworkflows/local/trim_workflow.nf b/subworkflows/local/trim_workflow.nf
deleted file mode 100644
index 27088a180..000000000
--- a/subworkflows/local/trim_workflow.nf
+++ /dev/null
@@ -1,42 +0,0 @@
-include { FASTP }                       from '../../modules/nf-core/fastp/main'
-include { FASTQC as FASTQC_FOR_FASTP }  from '../../modules/nf-core/fastqc/main'
-
-
-workflow TRIM_WORKFLOW {
-    take:
-        reads
-
-    main:
-        ch_versions = Channel.empty()
-        ch_fastp_html = Channel.empty()
-        ch_fastp_json = Channel.empty()
-        ch_fastqc_trimmed = Channel.empty()
-
-        if (params.fastp_trim) {
-            FASTP(reads, params.adapter_fasta, false, false, false)
-            ch_versions = ch_versions.mix(FASTP.out.versions)
-
-            FASTQC_FOR_FASTP(FASTP.out.reads)
-            ch_versions = ch_versions.mix(FASTQC_FOR_FASTP.out.versions)
-
-            ch_reads_all = FASTP.out.reads
-            ch_reads_fusioncatcher = ch_reads_all
-            ch_fastp_html = FASTP.out.html
-            ch_fastp_json = FASTP.out.json
-            ch_fastqc_trimmed = FASTQC_FOR_FASTP.out.zip
-
-        }
-        else {
-            ch_reads_all = reads
-            ch_reads_fusioncatcher = reads
-        }
-
-    emit:
-        ch_reads_all
-        ch_reads_fusioncatcher
-        ch_fastp_html
-        ch_fastp_json
-        ch_fastqc_trimmed
-        versions = ch_versions
-    }
-
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 1f7890b54..bafea68c3 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -22,23 +22,25 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi
 include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 
 
-ch_chrgtf = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
-ch_rrna_interval = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
-ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
+ch_chrgtf                     = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect()
+ch_starindex_ref              = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
+ch_starindex_ensembl_ref      = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
+ch_refflat                    = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
+ch_rrna_interval              = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
+ch_adapter_fastp              = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta, checkIfExists: true) : Channel.empty()
+ch_fusionreport_ref           = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
+ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
+ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
 ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
-ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
-ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
+ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
+ch_hgnc_ref     = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
+ch_hgnc_date    = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
+ch_fasta        = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
+ch_gtf          = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
 ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
-ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
-ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
+ch_transcript   = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
+ch_fai          = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
+
 
 
 /*
@@ -51,6 +53,7 @@ workflow RNAFUSION {
 
     take:
     ch_samplesheet // channel: samplesheet read in from --input
+
     main:
 
     ch_versions = Channel.empty()
@@ -67,11 +70,13 @@ workflow RNAFUSION {
 
 
     TRIM_WORKFLOW (
-        ch_samplesheet
+        ch_samplesheet,
+        ch_adapter_fastp,
+        params.fastp_trim
     )
     ch_reads_fusioncatcher = TRIM_WORKFLOW.out.ch_reads_fusioncatcher
-    ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all
-    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+    ch_reads_all           = TRIM_WORKFLOW.out.ch_reads_all
+    ch_versions            = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
 
     SALMON_QUANT( ch_reads_all, ch_salmon_index.map{ meta, index ->  index  }, ch_gtf.map{ meta, gtf ->  gtf  }, [], false, 'A')

From 06ba247e6e624ff95aba80b65b90a09436ab419c Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 13:45:53 +0000
Subject: [PATCH 143/887] create snap

---
 .../trim_subworkflow/tests/main.nf.test.snap  | 29 +++++++++++++++++++
 1 file changed, 29 insertions(+)
 create mode 100644 subworkflows/local/trim_subworkflow/tests/main.nf.test.snap

diff --git a/subworkflows/local/trim_subworkflow/tests/main.nf.test.snap b/subworkflows/local/trim_subworkflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..4623a3938
--- /dev/null
+++ b/subworkflows/local/trim_subworkflow/tests/main.nf.test.snap
@@ -0,0 +1,29 @@
+{
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP true": {
+        "content": [
+            [
+                "versions.yml:md5,16187796d989b6260f572247e7dc0fc6",
+                "versions.yml:md5,ea42abe9875f41f8362a55ee7533f102"
+            ],
+            [
+                "test_fastq_1.fastp.fastq.gz:md5,0c436583301dea48755a5252a2675b64",
+                "test_fastq_2.fastp.fastq.gz:md5,f7f38138255e63b33286b819b6177612"
+            ],
+            [
+                "test_fastq_1.fastp.fastq.gz:md5,0c436583301dea48755a5252a2675b64",
+                "test_fastq_2.fastp.fastq.gz:md5,f7f38138255e63b33286b819b6177612"
+            ],
+            "test_fastq.fastp.html",
+            "test_fastq.fastp.json:md5,62066ad48c3d5981045cdd43e354cb2b",
+            [
+                "test_fastq_trimmed_1_fastqc.zip",
+                "test_fastq_trimmed_2_fastqc.zip"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-05T13:42:33.340326167"
+    }
+}
\ No newline at end of file

From ea4487f0595c1f1d6baf850fd6b7db2d04c58e4f Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 13:53:28 +0000
Subject: [PATCH 144/887] fix folder name

---
 subworkflows/local/{trim_subworkflow => trim_workflow}/main.nf    | 0
 .../local/{trim_subworkflow => trim_workflow}/tests/main.nf.test  | 0
 .../{trim_subworkflow => trim_workflow}/tests/main.nf.test.snap   | 0
 3 files changed, 0 insertions(+), 0 deletions(-)
 rename subworkflows/local/{trim_subworkflow => trim_workflow}/main.nf (100%)
 rename subworkflows/local/{trim_subworkflow => trim_workflow}/tests/main.nf.test (100%)
 rename subworkflows/local/{trim_subworkflow => trim_workflow}/tests/main.nf.test.snap (100%)

diff --git a/subworkflows/local/trim_subworkflow/main.nf b/subworkflows/local/trim_workflow/main.nf
similarity index 100%
rename from subworkflows/local/trim_subworkflow/main.nf
rename to subworkflows/local/trim_workflow/main.nf
diff --git a/subworkflows/local/trim_subworkflow/tests/main.nf.test b/subworkflows/local/trim_workflow/tests/main.nf.test
similarity index 100%
rename from subworkflows/local/trim_subworkflow/tests/main.nf.test
rename to subworkflows/local/trim_workflow/tests/main.nf.test
diff --git a/subworkflows/local/trim_subworkflow/tests/main.nf.test.snap b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
similarity index 100%
rename from subworkflows/local/trim_subworkflow/tests/main.nf.test.snap
rename to subworkflows/local/trim_workflow/tests/main.nf.test.snap

From e033e9445a20a807651299d0b85c90de2eb441da Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 14:25:55 +0000
Subject: [PATCH 145/887] update test for skipping fastp

---
 .../local/trim_workflow/tests/main.nf.test    | 43 +++++++++++++++++--
 .../trim_workflow/tests/main.nf.test.snap     | 17 +++++++-
 2 files changed, 55 insertions(+), 5 deletions(-)

diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test b/subworkflows/local/trim_workflow/tests/main.nf.test
index 66007f0d4..854f9d4d2 100644
--- a/subworkflows/local/trim_workflow/tests/main.nf.test
+++ b/subworkflows/local/trim_workflow/tests/main.nf.test
@@ -2,7 +2,6 @@ nextflow_workflow {
 
     name "Test Subworkflow QC_WORKFLOW"
     script "../main.nf"
-    //config "./nextflow.config"
     workflow "TRIM_WORKFLOW"
     tag "qc"
     tag "subworkflow"
@@ -10,9 +9,6 @@ nextflow_workflow {
     test("TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP true") {
 
         when {
-            params {
-                fastp_trim = true
-            }
 
             workflow {
                 """
@@ -47,4 +43,43 @@ nextflow_workflow {
             )
         }
     }
+
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP false") {
+
+        when {
+
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [id: "test_fastq"],
+                        [
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
+                        ]
+                    ])
+                input[1] = Channel.empty()
+                input[2] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { with(workflow.out) {
+                    assert snapshot(
+                        versions.size() == 0,
+                        ch_reads_all[0][1].size() == 2,
+                        ch_reads_fusioncatcher[0][1].size() == 2,
+                        ch_fastp_html.size() == 0,
+                        ch_fastp_json.size() == 0,
+                        ch_fastqc_trimmed.size() == 0
+                    ).match() }
+                }
+            )
+        }
+    }
+
 }
diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test.snap b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
index 4623a3938..c119f7504 100644
--- a/subworkflows/local/trim_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
@@ -24,6 +24,21 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-05T13:42:33.340326167"
+        "timestamp": "2024-12-05T14:11:12.374063253"
+    },
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP false": {
+        "content": [
+            true,
+            true,
+            true,
+            true,
+            true,
+            true
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-05T14:21:42.396615145"
     }
 }
\ No newline at end of file

From 8f3c70e091df4f0d8f867a11c114ff7f3bd62e9e Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 14:29:30 +0000
Subject: [PATCH 146/887] update changelog

---
 CHANGELOG.md | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 11444abe0..6a70a4343 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -17,7 +17,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to nf-core module: `PICARD_COLLECTRNASEQMETRICS` and update module [#551](https://github.com/nf-core/rnafusion/pull/551)
 - Add `--skip_vcf` boolean parameter to skip vcf file generation [#554](https://github.com/nf-core/rnafusion/pull/554)
 - Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
-- Add nf-test to local subworkflow: `QC_SUBWORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
+- Add nf-test to local subworkflow: `QC_WORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
+- Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
 
 ### Changed
 

From cc916e6e551de9c49a2ea1a32593d6806906f88f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 5 Dec 2024 15:12:22 +0000
Subject: [PATCH 147/887] fix: change hardcoded container for seqera container,
 mv conda to environment.yml

---
 modules/local/fusioncatcher/download/environment.yml | 5 +++++
 modules/local/fusioncatcher/download/main.nf         | 4 ++--
 2 files changed, 7 insertions(+), 2 deletions(-)
 create mode 100644 modules/local/fusioncatcher/download/environment.yml

diff --git a/modules/local/fusioncatcher/download/environment.yml b/modules/local/fusioncatcher/download/environment.yml
new file mode 100644
index 000000000..7fbda99b9
--- /dev/null
+++ b/modules/local/fusioncatcher/download/environment.yml
@@ -0,0 +1,5 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::fusioncatcher
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 156e70b65..3164f1aa3 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -2,8 +2,8 @@ process FUSIONCATCHER_DOWNLOAD {
     tag "fusioncatcher_download"
     label 'process_medium'
 
-    conda "bioconda::fusioncatcher=1.33"
-    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
+    conda "${moduleDir}/environment.yml"
+    container "community.wave.seqera.io/library/fusioncatcher:e0ca700aac225cc8"
 
     output:
     path "*"                , emit: reference

From 2010578ec950e8ee149858ac5f902d491dfbde32 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 5 Dec 2024 15:13:41 +0000
Subject: [PATCH 148/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 11444abe0..8d6168afa 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -34,6 +34,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Refactor structure in `FUSIONREPORT_DOWNLOAD` to use cosmic credentials in `ext.args` [#556](https://github.com/nf-core/rnafusion/issues/556)
 - Fixed bug in nf-core `RRNATRANSCRIPTS` module [#563](https://github.com/nf-core/rnafusion/issues/563)
 - Fixed bug in `GFFREAD` that caused output `gffread_fasta` not being produced [#565](https://github.com/nf-core/rnafusion/issues/565)
+- Fixed bug in `FUSIONCATCHER_DOWNLOAD` that caused an error when running with singularity profile [#573](https://github.com/nf-core/rnafusion/issues/573)
 
 ### Removed
 

From ca0870bc3b9568b65458392293bb390c61866597 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 5 Dec 2024 15:20:28 +0000
Subject: [PATCH 149/887] fix: specify version 1.33 in bioconda and seqera
 container

---
 modules/local/fusioncatcher/detect/environment.yml   | 4 ++++
 modules/local/fusioncatcher/detect/main.nf           | 4 ++--
 modules/local/fusioncatcher/download/environment.yml | 3 +--
 modules/local/fusioncatcher/download/main.nf         | 2 +-
 4 files changed, 8 insertions(+), 5 deletions(-)
 create mode 100644 modules/local/fusioncatcher/detect/environment.yml

diff --git a/modules/local/fusioncatcher/detect/environment.yml b/modules/local/fusioncatcher/detect/environment.yml
new file mode 100644
index 000000000..312995854
--- /dev/null
+++ b/modules/local/fusioncatcher/detect/environment.yml
@@ -0,0 +1,4 @@
+channels:
+  - bioconda
+dependencies:
+  - bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 2977d6686..9a0928463 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -2,8 +2,8 @@ process FUSIONCATCHER {
     tag "$meta.id"
     label 'process_high'
 
-    conda "bioconda::fusioncatcher=1.33"
-    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
+    conda "${moduleDir}/environment.yml"
+    container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/fusioncatcher/download/environment.yml b/modules/local/fusioncatcher/download/environment.yml
index 7fbda99b9..312995854 100644
--- a/modules/local/fusioncatcher/download/environment.yml
+++ b/modules/local/fusioncatcher/download/environment.yml
@@ -1,5 +1,4 @@
 channels:
-  - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fusioncatcher
+  - bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 3164f1aa3..65a736077 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER_DOWNLOAD {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "community.wave.seqera.io/library/fusioncatcher:e0ca700aac225cc8"
+    container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
 
     output:
     path "*"                , emit: reference

From 90aced2d44603cb0440dd98dd858c0cd87db8f8b Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 16:24:35 +0000
Subject: [PATCH 150/887] add new test

---
 .../local/trim_workflow/tests/main.nf.test    | 44 ++++++++++++++++++-
 .../trim_workflow/tests/main.nf.test.snap     | 35 +++++++++++++--
 2 files changed, 73 insertions(+), 6 deletions(-)

diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test b/subworkflows/local/trim_workflow/tests/main.nf.test
index 854f9d4d2..2f7568d6d 100644
--- a/subworkflows/local/trim_workflow/tests/main.nf.test
+++ b/subworkflows/local/trim_workflow/tests/main.nf.test
@@ -5,8 +5,10 @@ nextflow_workflow {
     workflow "TRIM_WORKFLOW"
     tag "qc"
     tag "subworkflow"
+    tag "fastqc"
+    tag "fastp"
 
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP true") {
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true") {
 
         when {
 
@@ -44,7 +46,45 @@ nextflow_workflow {
         }
     }
 
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP false") {
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true + fasp_adaptors") {
+
+        when {
+
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [id: "test_fastq"],
+                        [
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
+                        ]
+                    ])
+                input[1] = Channel.fromPath('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/delete_me/fastp/adapters.fasta', checkIfExists: true)
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { with(workflow.out) {
+                    assert snapshot(
+                        versions.collect{ file(it) },
+                        ch_reads_all[0][1].collect { file(it) },
+                        ch_reads_fusioncatcher[0][1].collect { file(it) },
+                        file(ch_fastp_html[0][1]).name,
+                        file(ch_fastp_json[0][1]),
+                        ch_fastqc_trimmed[0][1].collect { file(it).name }
+                    ).match() }
+                }
+            )
+        }
+    }
+
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == false") {
 
         when {
 
diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test.snap b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
index c119f7504..ae9f9e2e0 100644
--- a/subworkflows/local/trim_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP true": {
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true": {
         "content": [
             [
                 "versions.yml:md5,16187796d989b6260f572247e7dc0fc6",
@@ -24,9 +24,9 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-05T14:11:12.374063253"
+        "timestamp": "2024-12-05T16:21:52.926289296"
     },
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - FastP false": {
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == false": {
         "content": [
             true,
             true,
@@ -39,6 +39,33 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-05T14:21:42.396615145"
+        "timestamp": "2024-12-05T16:22:45.877168833"
+    },
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true + fasp_adaptors": {
+        "content": [
+            [
+                "versions.yml:md5,16187796d989b6260f572247e7dc0fc6",
+                "versions.yml:md5,ea42abe9875f41f8362a55ee7533f102"
+            ],
+            [
+                "test_fastq_1.fastp.fastq.gz:md5,adc67a7b4d0bf3520866d7599a4ba814",
+                "test_fastq_2.fastp.fastq.gz:md5,9ee7d6c5230442970997477464255e67"
+            ],
+            [
+                "test_fastq_1.fastp.fastq.gz:md5,adc67a7b4d0bf3520866d7599a4ba814",
+                "test_fastq_2.fastp.fastq.gz:md5,9ee7d6c5230442970997477464255e67"
+            ],
+            "test_fastq.fastp.html",
+            "test_fastq.fastp.json:md5,feb3483311bfa4ded60146f1cbc13fd5",
+            [
+                "test_fastq_trimmed_1_fastqc.zip",
+                "test_fastq_trimmed_2_fastqc.zip"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-05T16:22:26.29488483"
     }
 }
\ No newline at end of file

From 6189acf28d3a3fd43c5882490f6ed79fbac95f76 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 5 Dec 2024 19:38:19 +0000
Subject: [PATCH 151/887] refactor: emit a directory and add meta

---
 modules/local/fusionreport/download/main.nf   | 26 ++++----
 .../fusionreport/download/tests/main.nf.test  |  6 +-
 .../download/tests/main.nf.test.snap          | 66 +++++++++----------
 3 files changed, 48 insertions(+), 50 deletions(-)

diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 183d95602..b19a64462 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -6,18 +6,17 @@ process FUSIONREPORT_DOWNLOAD {
     container "docker.io/clinicalgenomics/fusion-report:3.1.0"
 
     output:
-    path "fusiongdb2.db"    , emit: fusiongdb2
-    path "mitelman.db"      , emit: mitelman
-    path "cosmic.db"        , emit: cosmic, optional: true
-    path "*.txt"            , emit: timestamp
-    path "*.log"            , emit: log
-    path "versions.yml"     , emit: versions
+    tuple val(meta), path("fusionreport_dbs"), emit: fusionreport_db
+    path "versions.yml"                      , emit: versions
 
     script:
+    meta = [id: 'fusionreport_dbs']
     def args = task.ext.args ?: ''
-    def args2 = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
     """
     fusion_report download $args ./
+    mkdir fusionreport_dbs
+    mv *.txt *.log *.db fusionreport_dbs
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -26,17 +25,18 @@ process FUSIONREPORT_DOWNLOAD {
     """
 
     stub:
+    meta = [id: 'fusionreport_dbs']
     """
-    touch cosmic.db
-    touch fusiongdb2.db
-    touch mitelman.db
-    touch DB-timestamp.txt
-    touch fusion_report.log
+    mkdir fusionreport_dbs
+    touch fusionreport_dbs/cosmic.db
+    touch fusionreport_dbs/fusiongdb2.db
+    touch fusionreport_dbs/mitelman.db
+    touch fusionreport_dbs/DB-timestamp.txt
+    touch fusionreport_dbs/fusion_report.log
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         fusion_report: \$(fusion_report --version | sed 's/fusion-report //')
     END_VERSIONS
     """
-
 }
diff --git a/modules/local/fusionreport/download/tests/main.nf.test b/modules/local/fusionreport/download/tests/main.nf.test
index 33486764a..dd079587f 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test
+++ b/modules/local/fusionreport/download/tests/main.nf.test
@@ -19,8 +19,10 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    process.out.fusiongdb2,
-                    process.out.mitelman,
+                    path(process.out.fusionreport_db[0][1]).resolve("fusiongdb2.db"),
+                    path(process.out.fusionreport_db[0][1]).resolve("mitelman.db"),
+                    path(process.out.fusionreport_db[0][1]).resolve("DB-timestamp.txt").exists(),
+                    path(process.out.fusionreport_db[0][1]).resolve("fusion_report.log").exists(),
                     process.out.versions
                     ).match() }
             )
diff --git a/modules/local/fusionreport/download/tests/main.nf.test.snap b/modules/local/fusionreport/download/tests/main.nf.test.snap
index d828fcf51..c328f7486 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test.snap
+++ b/modules/local/fusionreport/download/tests/main.nf.test.snap
@@ -1,12 +1,10 @@
 {
     "Download fusionreport databases": {
         "content": [
-            [
-                "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a"
-            ],
-            [
-                "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a"
-            ],
+            "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a",
+            "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a",
+            true,
+            true,
             [
                 "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
             ]
@@ -15,43 +13,41 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-02T13:09:56.930477745"
+        "timestamp": "2024-12-05T19:27:38.99855171"
     },
     "Create stub files": {
         "content": [
             {
                 "0": [
-                    "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    [
+                        {
+                            "id": "fusionreport_dbs"
+                        },
+                        [
+                            "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
                 ],
                 "1": [
-                    "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "2": [
-                    "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "3": [
-                    "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "4": [
-                    "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "5": [
                     "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
                 ],
-                "cosmic": [
-                    "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "fusiongdb2": [
-                    "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "log": [
-                    "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "mitelman": [
-                    "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "timestamp": [
-                    "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                "fusionreport_db": [
+                    [
+                        {
+                            "id": "fusionreport_dbs"
+                        },
+                        [
+                            "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
                 ],
                 "versions": [
                     "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
@@ -62,6 +58,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-02T13:10:12.577952658"
+        "timestamp": "2024-12-05T19:35:49.629287874"
     }
 }
\ No newline at end of file

From 2620dcfba89178955f8da8e2617b6b82cc657dfc Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 5 Dec 2024 20:12:53 +0000
Subject: [PATCH 152/887] test: add fusionreport nf-test

---
 .../fusionreport/detect/tests/main.nf.test    | 60 +++++++++++++++++++
 .../fusionreport/detect/tests/nextflow.config | 10 ++++
 2 files changed, 70 insertions(+)
 create mode 100644 modules/local/fusionreport/detect/tests/main.nf.test
 create mode 100644 modules/local/fusionreport/detect/tests/nextflow.config

diff --git a/modules/local/fusionreport/detect/tests/main.nf.test b/modules/local/fusionreport/detect/tests/main.nf.test
new file mode 100644
index 000000000..5d0d89aea
--- /dev/null
+++ b/modules/local/fusionreport/detect/tests/main.nf.test
@@ -0,0 +1,60 @@
+nextflow_process {
+
+    name "Test Process FUSIONREPORT"
+    script "../main.nf"
+    process "FUSIONREPORT"
+    tag "modules"
+    tag "modules_local"
+    tag "fusionreport"
+
+
+    test("FUSIONREPORT - arriba - starfusion - fusioncatcher") {
+        config './nextflow.config'
+
+        setup {
+            run("FUSIONREPORT_DOWNLOAD") {
+                script "../../../fusionreport/download/main.nf"
+                process {
+                    """
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+
+                input[0] = [
+                    [ id:'test_sample' ], // meta map
+                    file("https://github.com/atrigila/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz"),
+                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv"),
+                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv"),
+                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
+                ]
+
+                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_db
+
+                input[2] = 1
+                """
+            }
+            params {
+                arriba          = true
+                starfusion      = true
+                fusioncatcher   = true
+                no_cosmic       = true
+                outdir          = "$outputDir"
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+
+}
diff --git a/modules/local/fusionreport/detect/tests/nextflow.config b/modules/local/fusionreport/detect/tests/nextflow.config
new file mode 100644
index 000000000..a1c327073
--- /dev/null
+++ b/modules/local/fusionreport/detect/tests/nextflow.config
@@ -0,0 +1,10 @@
+process {
+    withName: 'FUSIONREPORT_DOWNLOAD' {
+        ext.args         =  "--no-cosmic"
+    }
+
+    withName: 'FUSIONREPORT' {
+        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : "" }
+        ext.args2        = "--export csv"
+    }
+}

From 9673b855044ce293a1e67a30b2199fe248b58180 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 20:58:39 +0000
Subject: [PATCH 153/887] update module

---
 modules.json                                  |   2 +-
 modules/nf-core/arriba/arriba/environment.yml |   3 +
 modules/nf-core/arriba/arriba/main.nf         |  17 ++-
 modules/nf-core/arriba/arriba/meta.yml        |  39 +------
 .../nf-core/arriba/arriba/tests/main.nf.test  | 107 ++++++++++++++++++
 .../arriba/arriba/tests/main.nf.test.snap     |  55 +++++++++
 .../arriba/arriba/tests/nextflow.config       |   8 ++
 7 files changed, 186 insertions(+), 45 deletions(-)
 create mode 100644 modules/nf-core/arriba/arriba/tests/main.nf.test
 create mode 100644 modules/nf-core/arriba/arriba/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/arriba/arriba/tests/nextflow.config

diff --git a/modules.json b/modules.json
index 15c6ecac1..f8ae46aeb 100644
--- a/modules.json
+++ b/modules.json
@@ -12,7 +12,7 @@
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "7741dfc830e77a8ead2fcb50b01461ee09d0cdfe",
                         "installed_by": ["modules"]
                     },
                     "arriba/download": {
diff --git a/modules/nf-core/arriba/arriba/environment.yml b/modules/nf-core/arriba/arriba/environment.yml
index d0883a0d2..c3a88ffbd 100644
--- a/modules/nf-core/arriba/arriba/environment.yml
+++ b/modules/nf-core/arriba/arriba/environment.yml
@@ -1,5 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
+
 dependencies:
   - bioconda::arriba=2.4.0
diff --git a/modules/nf-core/arriba/arriba/main.nf b/modules/nf-core/arriba/arriba/main.nf
index 761d0bf35..75dbf93ad 100644
--- a/modules/nf-core/arriba/arriba/main.nf
+++ b/modules/nf-core/arriba/arriba/main.nf
@@ -8,14 +8,13 @@ process ARRIBA_ARRIBA {
         'biocontainers/arriba:2.4.0--h0033a41_2' }"
 
     input:
-    tuple val(meta), path(bam)
+    tuple val(meta),  path(bam)
     tuple val(meta2), path(fasta)
     tuple val(meta3), path(gtf)
-    tuple val(meta4), path(blacklist)
-    tuple val(meta5), path(known_fusions)
-    tuple val(meta6), path(structural_variants)
-    tuple val(meta7), path(tags)
-    tuple val(meta8), path(protein_domains)
+    path(blacklist)
+    path(known_fusions)
+    path(cytobands)
+    path(protein_domains)
 
     output:
     tuple val(meta), path("*.fusions.tsv")          , emit: fusions
@@ -30,8 +29,7 @@ process ARRIBA_ARRIBA {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
     def known_fusions = known_fusions ? "-k $known_fusions" : ""
-    def structural_variants = structural_variants ? "-d $structual_variants" : ""
-    def tags = tags ? "-t $tags" : ""
+    def cytobands = cytobands ? "-d $cytobands" : ""
     def protein_domains = protein_domains ? "-p $protein_domains" : ""
 
     """
@@ -43,8 +41,7 @@ process ARRIBA_ARRIBA {
         -O ${prefix}.fusions.discarded.tsv \\
         $blacklist \\
         $known_fusions \\
-        $structural_variants \\
-        $tags \\
+        $cytobands \\
         $protein_domains \\
         $args
 
diff --git a/modules/nf-core/arriba/arriba/meta.yml b/modules/nf-core/arriba/arriba/meta.yml
index f230dda1f..91ba21039 100644
--- a/modules/nf-core/arriba/arriba/meta.yml
+++ b/modules/nf-core/arriba/arriba/meta.yml
@@ -43,48 +43,19 @@ input:
         type: file
         description: Annotation GTF file
         pattern: "*.{gtf}"
-  - - meta4:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - blacklist:
+  - - blacklist:
         type: file
         description: Blacklist file
         pattern: "*.{tsv}"
-  - - meta5:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - known_fusions:
+  - - known_fusions:
         type: file
         description: Known fusions file
         pattern: "*.{tsv}"
-  - - meta6:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - structural_variants:
-        type: file
-        description: Structural variants file
-        pattern: "*.{tsv}"
-  - - meta7:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - tags:
+  - - cytobands:
         type: file
-        description: Tags file
+        description: Cytobands file
         pattern: "*.{tsv}"
-  - - meta8:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - protein_domains:
+  - - protein_domains:
         type: file
         description: Protein domains file
         pattern: "*.{gff3}"
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test b/modules/nf-core/arriba/arriba/tests/main.nf.test
new file mode 100644
index 000000000..4cff86e50
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test
@@ -0,0 +1,107 @@
+
+nextflow_process {
+
+    name "Test Process ARRIBA_ARRIBA"
+    script "../main.nf"
+    process "ARRIBA_ARRIBA"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "arriba"
+    tag "arriba/arriba"
+    tag "arriba/download"
+    tag "star/genomegenerate"
+    tag "star/align"
+
+    setup {
+        config "./nextflow.config"
+        options "-stub"
+        run("ARRIBA_DOWNLOAD") {
+            script "../../../arriba/download/main.nf"
+            process {
+                """
+                input[0] = 'GRCh38'
+                """
+            }
+        }
+        run("STAR_GENOMEGENERATE") {
+            script "../../../star/genomegenerate/main.nf"
+            options "-stub"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+        run("STAR_ALIGN") {
+            script "../../../star/align/main.nf"
+            options "-stub"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = false
+                input[4] = 'illumina'
+                input[5] = false
+                """
+            }
+        }
+    }
+
+    test("homo_sapiens - paired_end - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.bam', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = []
+
+                input[4] = []
+
+                input[5] = []
+
+                input[6] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test.snap b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
new file mode 100644
index 000000000..14d46f6b8
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
@@ -0,0 +1,55 @@
+{
+    "homo_sapiens - paired_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d323796555db4a58fe4c6bc08d1dec30"
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "fusions_fail": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d323796555db4a58fe4c6bc08d1dec30"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-10-08T15:41:23.945072"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/arriba/arriba/tests/nextflow.config b/modules/nf-core/arriba/arriba/tests/nextflow.config
new file mode 100644
index 000000000..d3d5b00f6
--- /dev/null
+++ b/modules/nf-core/arriba/arriba/tests/nextflow.config
@@ -0,0 +1,8 @@
+process {
+    withName: STAR_GENOMEGENERATE {
+        ext.args = '--genomeSAindexNbases 11'
+    }
+    withName: STAR_ALIGN {
+        ext.args = '--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50'
+    }
+}

From 275889eb49b88552539358a7c98496eca9a403e1 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Thu, 5 Dec 2024 21:02:17 +0000
Subject: [PATCH 154/887] update subworkflow

---
 subworkflows/local/arriba_workflow.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index ce142a749..ccc888231 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -28,7 +28,7 @@ workflow ARRIBA_WORKFLOW {
                     .map { meta, reads, fusions -> [ meta, fusions ] }
                 ch_arriba_fusion_fail = ch_dummy_file
             } else {
-                ARRIBA_ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, ch_arriba_ref_blacklist, ch_arriba_ref_known_fusions, [[],[]], [[],[]], ch_arriba_ref_protein_domains )
+                ARRIBA_ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, ch_arriba_ref_blacklist, ch_arriba_ref_known_fusions, [[],[]], ch_arriba_ref_protein_domains )
                 ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
                 ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions

From d290fcdc0513cf1fdfc5f97c4e06d43d02829d8f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 6 Dec 2024 10:56:21 +0100
Subject: [PATCH 155/887] update fusioncatcher container

---
 modules/local/fusioncatcher/build/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index f6ff09878..4aaaf504e 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -2,7 +2,7 @@ process FUSIONCATCHER_BUILD {
     tag "fusioncatcher_build"
     label 'process_medium'
 
-    container "docker.io/clinicalgenomics/fusioncatcher:1.33"
+    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
 
     input:
     val genome_gencode_version

From 75b65f7b19ca805195834ea2ae0bdc445368b9ee Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 6 Dec 2024 14:28:30 +0000
Subject: [PATCH 156/887] test: add test to `FUSIONREPORT_DETECT`

---
 .../fusionreport/detect/tests/main.nf.test    |  61 +++++-
 .../detect/tests/main.nf.test.snap            | 186 ++++++++++++++++++
 2 files changed, 245 insertions(+), 2 deletions(-)
 create mode 100644 modules/local/fusionreport/detect/tests/main.nf.test.snap

diff --git a/modules/local/fusionreport/detect/tests/main.nf.test b/modules/local/fusionreport/detect/tests/main.nf.test
index 5d0d89aea..bd5ab356a 100644
--- a/modules/local/fusionreport/detect/tests/main.nf.test
+++ b/modules/local/fusionreport/detect/tests/main.nf.test
@@ -27,7 +27,7 @@ nextflow_process {
 
                 input[0] = [
                     [ id:'test_sample' ], // meta map
-                    file("https://github.com/atrigila/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz"),
+                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz"),
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv"),
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv"),
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
@@ -50,11 +50,68 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out).match() }
+                { assert snapshot(
+                    process.out.fusion_list,
+                    process.out.fusion_list_filtered,
+                    process.out.csv,
+                    file(process.out.report[0][1]).name,
+                    process.out.html[0][1].collect { file(it).name },
+                    process.out.json,
+                    process.out.versions.flatten().first().toString().split('/')[-1]  // md5sum not stable as versions contains DB timestamp
+                    ).match() }
             )
         }
 
     }
 
+    test("FUSIONREPORT - arriba - starfusion - fusioncatcher - stub") {
+        config './nextflow.config'
+
+        setup {
+            run("FUSIONREPORT_DOWNLOAD") {
+                script "../../../fusionreport/download/main.nf"
+                process {
+                    """
+                    """
+                }
+            }
+        }
+
+        options "-stub"
+
+        when {
+            process {
+                """
+
+                input[0] = [
+                    [ id:'test_sample' ], // meta map
+                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz"),
+                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv"),
+                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv"),
+                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
+                ]
+
+                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_db
+
+                input[2] = 1
+                """
+            }
+        params {
+                arriba          = true
+                starfusion      = true
+                fusioncatcher   = true
+                no_cosmic       = true
+                outdir          = "$outputDir"
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
 
 }
diff --git a/modules/local/fusionreport/detect/tests/main.nf.test.snap b/modules/local/fusionreport/detect/tests/main.nf.test.snap
new file mode 100644
index 000000000..fb37037fa
--- /dev/null
+++ b/modules/local/fusionreport/detect/tests/main.nf.test.snap
@@ -0,0 +1,186 @@
+{
+    "FUSIONREPORT - arriba - starfusion - fusioncatcher": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "test_sample.fusionreport.tsv:md5,3593b7021f26cc5427fdc96f0d1c72f0"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "test_sample.fusionreport_filtered.tsv:md5,3593b7021f26cc5427fdc96f0d1c72f0"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "test_sample.fusions.csv:md5,49f378c2112d7e0b3b17d9095c79e6bd"
+                ]
+            ],
+            "test_sample_fusionreport_index.html",
+            [
+                "AKAP9_BRAF.html",
+                "BRD4-1_NUTM1.html",
+                "BRD4_NUTM1.html",
+                "CD74_AL132671.2.html",
+                "CD74_ROS1.html",
+                "CIC_DUX4.html",
+                "DUX4_IGH@.html",
+                "EML4_ALK.html",
+                "ETV6_NTRK3.html",
+                "EWSR1_ATF1.html",
+                "EWSR1_FLI1.html",
+                "FGFR3_TACC3.html",
+                "FIP1L1_PDGFRA.html",
+                "GOPC_ROS1.html",
+                "HOOK3_RET.html",
+                "IGH@_CRLF2.html",
+                "MALT1_IGH@.html",
+                "NPM1_ALK.html",
+                "TMPRSS2_ETV1.html",
+                "test_sample_fusionreport_index.html"
+            ],
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "fusion_genes_mqc.json:md5,b196dc8d064a47d17fd3a032b8fbed4f"
+                ]
+            ],
+            "versions.yml"
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-06T14:23:44.59690452"
+    },
+    "FUSIONREPORT - arriba - starfusion - fusioncatcher - stub": {
+        "content": [
+            {
+                "0": [
+                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusionreport.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusionreport_filtered.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        [
+                            "AAA_BBB.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_sample_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusions.csv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusions.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "csv": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusions.csv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fusion_list": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusionreport.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fusion_list_filtered": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusionreport_filtered.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        [
+                            "AAA_BBB.html:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test_sample_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "json": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample.fusions.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "report": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-06T13:12:32.981394201"
+    }
+}
\ No newline at end of file

From 2c860bb2d80db5aa197ec9dd94b4d91adf2a1cba Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 6 Dec 2024 14:28:38 +0000
Subject: [PATCH 157/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 6a70a4343..14eb29741 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
 - Add nf-test to local subworkflow: `QC_WORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
+- Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/572)
 
 ### Changed
 

From e80fd835aa2eef94862d59dde5f535e3f0272bb0 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Fri, 6 Dec 2024 14:31:45 +0000
Subject: [PATCH 158/887] change module structure and first test attempt

---
 ...iba_workflow.nf => arriba_workflow.nf.bkp} |   0
 subworkflows/local/arriba_workflow/main.nf    |  92 ++++++++++++
 .../local/arriba_workflow/tests/main.nf.test  | 136 ++++++++++++++++++
 workflows/rnafusion.nf                        |  10 +-
 4 files changed, 237 insertions(+), 1 deletion(-)
 rename subworkflows/local/{arriba_workflow.nf => arriba_workflow.nf.bkp} (100%)
 create mode 100644 subworkflows/local/arriba_workflow/main.nf
 create mode 100644 subworkflows/local/arriba_workflow/tests/main.nf.test

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf.bkp
similarity index 100%
rename from subworkflows/local/arriba_workflow.nf
rename to subworkflows/local/arriba_workflow.nf.bkp
diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
new file mode 100644
index 000000000..a19fe86b2
--- /dev/null
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -0,0 +1,92 @@
+include { ARRIBA_ARRIBA                               } from '../../../modules/nf-core/arriba/arriba/main'
+include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_ARRIBA } from '../../../modules/nf-core/samtools/index/main'
+include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA   } from '../../../modules/nf-core/samtools/sort/main'
+include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_ARRIBA   } from '../../../modules/nf-core/samtools/view/main'
+include { STAR_ALIGN as STAR_FOR_ARRIBA               } from '../../../modules/nf-core/star/align/main'
+
+workflow ARRIBA_WORKFLOW {
+    take:
+        reads                           // channel [ meta, [ fastqs ]         ]
+        ch_gtf                          // channel [ meta, path_gtf           ]
+        ch_fasta                        // channel [ meta, path_fasta         ]
+        ch_starindex_ref                // channel [ meta, path_index         ]
+        ch_arriba_ref_blacklist         // channel [ meta, path_blacklist     ]
+        ch_arriba_ref_known_fusions     // channel [ meta, path_known_fusions ]
+        ch_arriba_ref_cytobands         // channel [ meta, path_cytobands     ]
+        ch_arriba_ref_protein_domains   // channel [ meta, path_proteins      ]
+        arriba                          // boolean
+        all                             // boolean
+        fusioninspector_only            // boolean
+        star_ignore_sjdbgtf             // boolean
+        seq_center                      // string
+        arriba_fusions                  // path?
+        cram                            // array?
+
+    main:
+        ch_versions = Channel.empty()
+        ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
+
+        if (( arriba || all) && !fusioninspector_only) {
+
+            STAR_FOR_ARRIBA(
+                reads,
+                ch_starindex_ref,
+                ch_gtf,
+                star_ignore_sjdbgtf,
+                '',                       // seq_platform, should be a params like other pipelines
+                seq_center
+            )
+
+            ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+
+            if ( arriba_fusions ) {
+
+                ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) ) // This should be done in the main scirpt?
+                    .map { meta, reads, fusions -> [ meta, fusions ] }
+                ch_arriba_fusion_fail = ch_dummy_file
+
+            } else {
+
+                ARRIBA_ARRIBA (
+                    STAR_FOR_ARRIBA.out.bam,
+                    ch_fasta,
+                    ch_gtf,
+                    ch_arriba_ref_blacklist.map{ it[1] },       // should we update nf-core module to includes meta?
+                    ch_arriba_ref_known_fusions.map{ it[1] },
+                    ch_arriba_ref_cytobands.map{ it[1] },
+                    ch_arriba_ref_protein_domains.map{ it[1] }
+                )
+
+                ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
+
+                ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
+                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ meta, file -> return file}
+            }
+
+            if ( cram.contains('arriba') ) {
+
+                SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam, ch_fasta)
+                ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions )
+
+                SAMTOOLS_VIEW_FOR_ARRIBA(SAMTOOLS_SORT_FOR_ARRIBA.out.bam.map { meta, bam -> [ meta, bam, [] ] }, ch_fasta, [])
+                ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_ARRIBA.out.versions )
+
+                SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_VIEW_FOR_ARRIBA.out.cram)
+                ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions )
+
+            }
+
+        }
+        else {
+            ch_arriba_fusions       = reads.combine(Channel.value( file(ch_dummy_file, checkIfExists:true ) ) )
+                                        .map { meta, reads, fusions -> [ meta, fusions ] }
+
+            ch_arriba_fusion_fail   = ch_dummy_file
+        }
+
+    emit:
+        fusions         = ch_arriba_fusions
+        fusions_fail    = ch_arriba_fusion_fail
+        versions        = ch_versions
+    }
+
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
new file mode 100644
index 000000000..73f91ea28
--- /dev/null
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test
@@ -0,0 +1,136 @@
+nextflow_workflow {
+
+    name "Test Subworkflow ARRIBA_WORKFLOW"
+    script "../main.nf"
+    //config "./nextflow.config"
+    workflow "ARRIBA_WORKFLOW"
+    tag "subworkflow"
+    tag "arriba"
+    tag "arriba/arriba"
+    tag "samtools"
+    tag "samtools/index"
+    tag "samtools/sort"
+    tag "samtools/view"
+    tag "star"
+    tag "star/align"
+
+
+    test("ARRIBA_WORKFLOW - Homo sapiens chr22") {
+
+        when {
+            setup {
+                // Create genome index for STAR
+                run("STAR_GENOMEGENERATE") {
+                    script "../../../../modules/nf-core/star/genomegenerate/main"
+                    process {
+                        """
+                        // FASTA
+                        input[0] = Channel.fromPath(
+                                "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExist: true
+                            )
+                            .map{ [[id: it.name], it ]}
+
+
+                        // GTF
+                        input[1] = Channel.fromPath(
+                                "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
+                            )
+                            .map{ [[id: it.name], it ]}
+                        """
+                    }
+                }
+            }
+
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [ id: "test_fastqs" ],
+                        [
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
+                        ]
+                    ] )
+
+                // ch_gtf
+                input[1] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_fasta
+                input[2] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExist: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_starindex_ref
+                input[3] = STAR_GENOMEGENERATE.out.index.map{ [ [id: "star_index"], it ] }
+
+                // ch_arriba_ref_blacklist
+                input[4] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExist: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_known_fusions
+                input[5] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExist: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_cytobands
+                input[6] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExist: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_protein_domains
+                input[7] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExist: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // arriba (boolean)
+                input[8] = true
+
+                // all (boolean)
+                input[9] = true
+
+                // fusioninspector_only  (boolean)
+                input[10] = false
+
+                // star_ignore_sjdbgtf (boolean)
+                input[11] = false
+
+                // seq_center (string)
+                input[12] = 'test_center'
+
+                // arriba_fusions (path)
+                input[13] = null
+
+                // cram (array??)
+                input[14] = [ 'arriba' ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success }/*,
+                { assert snapshot(file( workflow.out.versions[0] )).match('versions' )                                                },
+                { assert snapshot(file( workflow.out.rnaseq_metrics[0][1]     ).readLines()[4..-1]).md5().match('rnaseq_metrics'    ) },
+                { assert snapshot(file( workflow.out.duplicate_metrics[0][1]  ).readLines()[4..-1]).md5().match('duplicate_metrics' ) },
+                { assert snapshot(file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1]).md5().match('insertsize_metrics') }*/
+            )
+        }
+    }
+
+}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bafea68c3..a457a1e3c 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -92,7 +92,15 @@ workflow RNAFUSION {
         ch_starindex_ensembl_ref,
         ch_arriba_ref_blacklist,
         ch_arriba_ref_known_fusions,
-        ch_arriba_ref_protein_domains
+        ch_arriba_ref_cytobands,
+        ch_arriba_ref_protein_domains,
+        params.arriba                   // boolean
+        params.all                      // boolean
+        params.fusioninspector_only     // boolean
+        params.star_ignore_sjdbgtf,
+        params.seq_center ?: '',
+        params.arriba_fusions,          // path
+        params.cram                     // array?
     )
     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
 

From a6c082ef6eb4fb0bb050b4fb7a1f2d6fa99c8cdf Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Fri, 6 Dec 2024 18:46:23 +0000
Subject: [PATCH 159/887] update test and generate first snap

---
 subworkflows/local/arriba_workflow/main.nf    | 17 +++--
 .../local/arriba_workflow/tests/main.nf.test  | 67 +++++++++----------
 .../arriba_workflow/tests/main.nf.test.snap   | 49 ++++++++++++++
 3 files changed, 91 insertions(+), 42 deletions(-)
 create mode 100644 subworkflows/local/arriba_workflow/tests/main.nf.test.snap

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index a19fe86b2..5460e9263 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -60,7 +60,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
                 ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
-                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ meta, file -> return file}
+                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ meta, file -> return file }
             }
 
             if ( cram.contains('arriba') ) {
@@ -78,15 +78,18 @@ workflow ARRIBA_WORKFLOW {
 
         }
         else {
-            ch_arriba_fusions       = reads.combine(Channel.value( file(ch_dummy_file, checkIfExists:true ) ) )
-                                        .map { meta, reads, fusions -> [ meta, fusions ] }
+            // Not sure how this dummy file can be useful
+            // If this tool can be skipped, why not just emitting an empty channel?
+            ch_arriba_fusions = reads
+                .combine(Channel.value( file(ch_dummy_file, checkIfExists: true ) ) )
+                .map { meta, reads, fusions -> [ meta, fusions ] }
 
-            ch_arriba_fusion_fail   = ch_dummy_file
+            ch_arriba_fusion_fail = ch_dummy_file
         }
 
     emit:
-        fusions         = ch_arriba_fusions
-        fusions_fail    = ch_arriba_fusion_fail
-        versions        = ch_versions
+        fusions      = ch_arriba_fusions
+        fusions_fail = ch_arriba_fusion_fail
+        versions     = ch_versions
     }
 
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
index 73f91ea28..1e713fbb1 100644
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test
@@ -2,7 +2,6 @@ nextflow_workflow {
 
     name "Test Subworkflow ARRIBA_WORKFLOW"
     script "../main.nf"
-    //config "./nextflow.config"
     workflow "ARRIBA_WORKFLOW"
     tag "subworkflow"
     tag "arriba"
@@ -12,35 +11,35 @@ nextflow_workflow {
     tag "samtools/sort"
     tag "samtools/view"
     tag "star"
+    tag "star/genomegenerate"
     tag "star/align"
 
+    test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4") {
 
-    test("ARRIBA_WORKFLOW - Homo sapiens chr22") {
-
-        when {
-            setup {
-                // Create genome index for STAR
-                run("STAR_GENOMEGENERATE") {
-                    script "../../../../modules/nf-core/star/genomegenerate/main"
-                    process {
-                        """
-                        // FASTA
-                        input[0] = Channel.fromPath(
-                                "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExist: true
-                            )
-                            .map{ [[id: it.name], it ]}
-
-
-                        // GTF
-                        input[1] = Channel.fromPath(
-                                "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
-                            )
-                            .map{ [[id: it.name], it ]}
-                        """
-                    }
+        setup {
+            // Create genome index for STAR
+            run("STAR_GENOMEGENERATE") {
+                script "../../../../modules/nf-core/star/genomegenerate/main"
+                process {
+                """
+                    // FASTA
+                    input[0] = Channel.fromPath(
+                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
+                            //"/workspace/rnafusion/results/test.fa", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+
+                    // GTF
+                    input[1] = Channel.fromPath(
+                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+                    """
                 }
             }
+        }
 
+        when {
             workflow {
                 """
                 // ch_reads
@@ -63,38 +62,39 @@ nextflow_workflow {
                 // ch_fasta
                 input[2] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExist: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
+                        //"/workspace/rnafusion/results/test.fa", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_starindex_ref
-                input[3] = STAR_GENOMEGENERATE.out.index.map{ [ [id: "star_index"], it ] }
+                input[3] = STAR_GENOMEGENERATE.out.index
 
                 // ch_arriba_ref_blacklist
                 input[4] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExist: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_known_fusions
                 input[5] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExist: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_cytobands
                 input[6] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExist: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_protein_domains
                 input[7] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExist: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
@@ -124,11 +124,8 @@ nextflow_workflow {
 
         then {
             assertAll(
-                { assert workflow.success }/*,
-                { assert snapshot(file( workflow.out.versions[0] )).match('versions' )                                                },
-                { assert snapshot(file( workflow.out.rnaseq_metrics[0][1]     ).readLines()[4..-1]).md5().match('rnaseq_metrics'    ) },
-                { assert snapshot(file( workflow.out.duplicate_metrics[0][1]  ).readLines()[4..-1]).md5().match('duplicate_metrics' ) },
-                { assert snapshot(file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1]).md5().match('insertsize_metrics') }*/
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
             )
         }
     }
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..f7b6a8db3
--- /dev/null
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
@@ -0,0 +1,49 @@
+{
+    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
+                    ]
+                ],
+                "1": [
+                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
+                ],
+                "2": [
+                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
+                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
+                    "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
+                    "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
+                    "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
+                    ]
+                ],
+                "fusions_fail": [
+                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
+                ],
+                "versions": [
+                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
+                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
+                    "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
+                    "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
+                    "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-06T18:40:22.919178866"
+    }
+}
\ No newline at end of file

From be6e10262720181536a80ca56fe12a79227ef815 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 9 Dec 2024 12:42:59 +0100
Subject: [PATCH 160/887] fix linting issues

---
 modules.json                                  |  6 +-
 .../local/get_rrna_transcript/environment.yml |  6 ++
 .../main.nf}                                  | 10 +--
 .../local/uscs/custom_gtftogenepred/main.nf   |  3 +-
 modules/nf-core/salmon/quant/main.nf          |  3 +-
 subworkflows/local/build_references.nf        | 22 +++--
 subworkflows/local/fusionreport_workflow.nf   |  2 +-
 .../nf-core/utils_nextflow_pipeline/main.nf   |  2 +
 .../tests/main.workflow.nf.test               | 10 ++-
 .../nf-core/utils_nfcore_pipeline/main.nf     | 89 +++++--------------
 .../tests/main.function.nf.test               | 46 ++++------
 .../tests/main.function.nf.test.snap          | 30 -------
 .../utils_nfschema_plugin/tests/main.nf.test  |  4 +-
 13 files changed, 86 insertions(+), 147 deletions(-)
 create mode 100644 modules/local/get_rrna_transcript/environment.yml
 rename modules/local/{get_rrna_transcripts.nf => get_rrna_transcript/main.nf} (79%)

diff --git a/modules.json b/modules.json
index 48fa1f6bd..b41d5fa59 100644
--- a/modules.json
+++ b/modules.json
@@ -152,17 +152,17 @@
                 "nf-core": {
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082",
+                        "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba",
+                        "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c",
+                        "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/local/get_rrna_transcript/environment.yml b/modules/local/get_rrna_transcript/environment.yml
new file mode 100644
index 000000000..66b65c3a9
--- /dev/null
+++ b/modules/local/get_rrna_transcript/environment.yml
@@ -0,0 +1,6 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::pirate=1.0.5
+  - bioconda::perl-bioperl=1.7.8
diff --git a/modules/local/get_rrna_transcripts.nf b/modules/local/get_rrna_transcript/main.nf
similarity index 79%
rename from modules/local/get_rrna_transcripts.nf
rename to modules/local/get_rrna_transcript/main.nf
index cb39b3e4c..5331f534a 100644
--- a/modules/local/get_rrna_transcripts.nf
+++ b/modules/local/get_rrna_transcript/main.nf
@@ -2,17 +2,17 @@ process GET_RRNA_TRANSCRIPTS {
     tag 'get_rrna_bed'
     label 'process_low'
 
-    conda "bioconda::pirate=1.0.4 bioconda::perl-bioperl=1.7.2"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/pirate:1.0.4--hdfd78af_2' :
-        'biocontainers/pirate:1.0.4--hdfd78af_2' }"
+        'https://depot.galaxyproject.org/singularity/pirate:1.0.5--hdfd78af_0' :
+        'biocontainers/pirate:1.0.5--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(gtf)
 
     output:
-    path('rrna.gtf')    , emit: rrnagtf
-    path('rrna.bed')    , emit: bed
+    tuple val(meta), path('rrna.gtf')    , emit: rrnagtf
+    tuple val(meta), path('rrna.bed')    , emit: bed
     path "versions.yml" , emit: versions
 
     when:
diff --git a/modules/local/uscs/custom_gtftogenepred/main.nf b/modules/local/uscs/custom_gtftogenepred/main.nf
index 53a74e3d3..9cc15765e 100644
--- a/modules/local/uscs/custom_gtftogenepred/main.nf
+++ b/modules/local/uscs/custom_gtftogenepred/main.nf
@@ -11,7 +11,8 @@ process GTF_TO_REFFLAT {
     tuple val(meta), path (gtf)
 
     output:
-    path('*.refflat'), emit: refflat
+    path('*.refflat')    , emit: refflat
+    path "versions.yml"  , emit: versions
 
     script:
     def genepred = gtf + '.genepred'
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index e2d273525..f1e3b5cda 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -29,8 +29,7 @@ process SALMON_QUANT {
     prefix   = task.ext.prefix ?: "${meta.id}"
 
     def reference   = "--index $index"
-    def reads1 = []
-    def reads2 = []
+    def reads1 = [], reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def input_reads = meta.single_end ? "-r ${reads1.join(" ")}" : "-1 ${reads1.join(" ")} -2 ${reads2.join(" ")}"
     if (alignment_mode) {
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 7d942601c..8685feebc 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -5,15 +5,13 @@
 */
 
 include { GENCODE_DOWNLOAD }                from '../../modules/local/gencode_download/main'
-include { FUSIONCATCHER_DOWNLOAD }          from '../../modules/local/fusioncatcher/download/main'
 include { FUSIONCATCHER_BUILD }             from '../../modules/local/fusioncatcher/build/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../../modules/local/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../../modules/local/starfusion/build/main'
-include { STARFUSION_DOWNLOAD }             from '../../modules/local/starfusion/download/main'
 include { GTF_TO_REFFLAT }                  from '../../modules/local/uscs/custom_gtftogenepred/main'
-include { GET_RRNA_TRANSCRIPTS }            from '../../modules/local/get_rrna_transcripts'
-// include { CONVERT2BED }                     from '../../modules/local/convert2bed/main'
+include { GET_RRNA_TRANSCRIPTS }            from '../../modules/local/get_rrna_transcript/main'
+
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
@@ -41,7 +39,7 @@ workflow BUILD_REFERENCES {
     if (!file(params.fasta).exists() || file(params.fasta).isEmpty() ||
             !file(params.gtf).exists() || file(params.gtf).isEmpty()){
         GENCODE_DOWNLOAD(params.genome_gencode_version, params.genome)
-        ch_versions = GENCODE_DOWNLOAD.out.versions
+        ch_versions = ch_versions.mix(GENCODE_DOWNLOAD.out.versions)
         ch_fasta = GENCODE_DOWNLOAD.out.fasta.map { that -> [[id:that.Name], that] }
         ch_gtf = GENCODE_DOWNLOAD.out.gtf.map { that -> [[id:that.Name], that] }
     } else {
@@ -51,7 +49,7 @@ workflow BUILD_REFERENCES {
 
     if (!file(params.fai).exists() || file(params.fai).isEmpty()){
         SAMTOOLS_FAIDX(ch_fasta, [[],[]])
-        ch_versions = SAMTOOLS_FAIDX.out.versions
+        ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions)
         ch_fai = SAMTOOLS_FAIDX.out.fai
     } else {
         ch_fai = Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
@@ -60,6 +58,7 @@ workflow BUILD_REFERENCES {
     if ((!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
             !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()) && !params.skip_vcf){
         HGNC_DOWNLOAD( )
+        ch_versions = ch_versions.mix(HGNC_DOWNLOAD.out.versions)
         ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref
         ch_hgnc_date = HGNC_DOWNLOAD.out.hgnc_date
     } else {
@@ -69,8 +68,11 @@ workflow BUILD_REFERENCES {
 
     if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty()){
         GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
+        ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
         GET_RRNA_TRANSCRIPTS(ch_gtf)
+        ch_versions = ch_versions.mix(GET_RRNA_TRANSCRIPTS.out.versions)
         GATK4_BEDTOINTERVALLIST(GET_RRNA_TRANSCRIPTS.out.bed.map { it -> [ [id:it.name], it ] }, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
+        ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions)
         ch_rrna_interval = GATK4_BEDTOINTERVALLIST.out.interval_list
     } else {
         ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
@@ -78,6 +80,7 @@ workflow BUILD_REFERENCES {
 
     if (!file(params.refflat).exists() || file(params.refflat).isEmpty()){
         GTF_TO_REFFLAT(ch_gtf)
+        ch_versions = ch_versions.mix(GTF_TO_REFFLAT.out.versions)
         ch_refflat = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
     } else {
         ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
@@ -86,7 +89,9 @@ workflow BUILD_REFERENCES {
     if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty() ||
         !file(params.salmon_index_stub_check).exists() || file(params.salmon_index_stub_check).isEmpty()){ // add condition for qc
         GFFREAD(ch_gtf, ch_fasta.map{ meta, fasta -> [ fasta ] })
+        ch_versions = ch_versions.mix(GFFREAD.out.versions)
         SALMON_INDEX(ch_fasta.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+        ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
         ch_salmon_index = SALMON_INDEX.out.index
     } else {
         ch_salmon_index = Channel.fromPath(params.salmon_index)
@@ -96,6 +101,7 @@ workflow BUILD_REFERENCES {
             (!file(params.starindex_ref).exists() || file(params.starindex_ref).isEmpty() ||
             !file(params.starindex_ref_stub_check).exists() || file(params.starindex_ref_stub_check).isEmpty() )) {
         STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
+        ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions)
         ch_starindex_ref = STAR_GENOMEGENERATE.out.index
     } else {
         ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
@@ -106,6 +112,7 @@ workflow BUILD_REFERENCES {
             !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
             !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
         ARRIBA_DOWNLOAD(params.genome)
+        ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)
         ch_arriba_ref_blacklist = ARRIBA_DOWNLOAD.out.blacklist
         ch_arriba_ref_cytobands = ARRIBA_DOWNLOAD.out.cytobands
         ch_arriba_ref_known_fusions = ARRIBA_DOWNLOAD.out.known_fusions
@@ -122,6 +129,7 @@ workflow BUILD_REFERENCES {
             (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
             !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
             FUSIONCATCHER_BUILD(params.genome_gencode_version)
+            ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
             ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
     }
     else {
@@ -133,6 +141,7 @@ workflow BUILD_REFERENCES {
             (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
             !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
             STARFUSION_BUILD(ch_fasta, ch_gtf)
+            ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             ch_starfusion_ref = STARFUSION_BUILD.out.reference
     }
     else {
@@ -145,6 +154,7 @@ workflow BUILD_REFERENCES {
             !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
         if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
         FUSIONREPORT_DOWNLOAD(params.cosmic_username, params.cosmic_passwd)
+        ch_versions = ch_versions.mix(FUSIONREPORT_DOWNLOAD.out.versions)
         ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.reference
     } else {
         ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref)
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index a61759f72..5c9bfde13 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -23,7 +23,7 @@ workflow FUSIONREPORT_WORKFLOW {
             FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
             ch_fusion_list = FUSIONREPORT.out.fusion_list
             ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered
-            // ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
+            ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
             ch_report = FUSIONREPORT.out.report
             ch_csv = FUSIONREPORT.out.csv
         } else {
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
index 0fcbf7b3f..d6e593e85 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -92,10 +92,12 @@ def checkCondaChannels() {
         channels = config.channels
     }
     catch (NullPointerException e) {
+        log.debug(e)
         log.warn("Could not verify conda channel configuration.")
         return null
     }
     catch (IOException e) {
+        log.debug(e)
         log.warn("Could not verify conda channel configuration.")
         return null
     }
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
index ca964ce8e..02dbf094c 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
@@ -52,10 +52,12 @@ nextflow_workflow {
         }
 
         then {
-            assertAll(
-                { assert workflow.success },
-                { assert workflow.stdout.contains("nextflow_workflow v9.9.9") }
-            )
+            expect {
+                with(workflow) {
+                    assert success
+                    assert "nextflow_workflow v9.9.9" in stdout
+                }
+            }
         }
     }
 
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index 5cb7bafef..bfd258760 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -56,21 +56,6 @@ def checkProfileProvided(nextflow_cli_args) {
     }
 }
 
-//
-// Citation string for pipeline
-//
-def workflowCitation() {
-    def temp_doi_ref = ""
-    def manifest_doi = workflow.manifest.doi.tokenize(",")
-    // Handling multiple DOIs
-    // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
-    // Removing ` ` since the manifest.doi is a string and not a proper list
-    manifest_doi.each { doi_ref ->
-        temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
-    }
-    return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + "* The nf-core framework\n" + "  https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
-}
-
 //
 // Generate workflow version string
 //
@@ -150,33 +135,6 @@ def paramsSummaryMultiqc(summary_params) {
     return yaml_file_text
 }
 
-//
-// nf-core logo
-//
-def nfCoreLogo(monochrome_logs=true) {
-    def colors = logColours(monochrome_logs) as Map
-    String.format(
-        """\n
-        ${dashedLine(monochrome_logs)}
-                                                ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
-        ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
-        ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
-        ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
-                                                ${colors.green}`._,._,\'${colors.reset}
-        ${colors.purple}  ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset}
-        ${dashedLine(monochrome_logs)}
-        """.stripIndent()
-    )
-}
-
-//
-// Return dashed line
-//
-def dashedLine(monochrome_logs=true) {
-    def colors = logColours(monochrome_logs) as Map
-    return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-}
-
 //
 // ANSII colours used for terminal logging
 //
@@ -245,28 +203,24 @@ def logColours(monochrome_logs=true) {
     return colorcodes
 }
 
-//
-// Attach the multiqc report to email
-//
-def attachMultiqcReport(multiqc_report) {
-    def mqc_report = null
-    try {
-        if (workflow.success) {
-            mqc_report = multiqc_report.getVal()
-            if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
-                if (mqc_report.size() > 1) {
-                    log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one")
-                }
-                mqc_report = mqc_report[0]
-            }
+// Return a single report from an object that may be a Path or List
+//
+def getSingleReport(multiqc_reports) {
+    if (multiqc_reports instanceof Path) {
+        return multiqc_reports
+    } else if (multiqc_reports instanceof List) {
+        if (multiqc_reports.size() == 0) {
+            log.warn("[${workflow.manifest.name}] No reports found from process 'MULTIQC'")
+            return null
+        } else if (multiqc_reports.size() == 1) {
+            return multiqc_reports.first()
+        } else {
+            log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one")
+            return multiqc_reports.first()
         }
+    } else {
+        return null
     }
-    catch (Exception all) {
-        if (multiqc_report) {
-            log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email")
-        }
-    }
-    return mqc_report
 }
 
 //
@@ -320,7 +274,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     email_fields['summary']      = summary << misc_fields
 
     // On success try attach the multiqc report
-    def mqc_report = attachMultiqcReport(multiqc_report)
+    def mqc_report = getSingleReport(multiqc_report)
 
     // Check if we are only sending emails on failure
     def email_address = email
@@ -340,7 +294,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     def email_html    = html_template.toString()
 
     // Render the sendmail template
-    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
+    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as MemoryUnit
     def smail_fields           = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()]
     def sf                     = new File("${workflow.projectDir}/assets/sendmail_template.txt")
     def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
@@ -351,14 +305,17 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     if (email_address) {
         try {
             if (plaintext_email) {
-new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML')            }
+                new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML')
+            }
             // Try to send HTML e-mail using sendmail
             def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
             sendmail_tf.withWriter { w -> w << sendmail_html }
             ['sendmail', '-t'].execute() << sendmail_html
             log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-")
         }
-        catch (Exception all) {
+        catch (Exception msg) {
+            log.debug(msg.toString())
+            log.debug("Trying with mail instead of sendmail")
             // Catch failures and try with plaintext
             def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address]
             mail_cmd.execute() << email_html
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
index 1dc317f8f..f117040cb 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
@@ -41,26 +41,14 @@ nextflow_function {
         }
     }
 
-    test("Test Function workflowCitation") {
-
-        function "workflowCitation"
-
-        then {
-            assertAll(
-                { assert function.success },
-                { assert snapshot(function.result).match() }
-            )
-        }
-    }
-
-    test("Test Function nfCoreLogo") {
+    test("Test Function without logColours") {
 
-        function "nfCoreLogo"
+        function "logColours"
 
         when {
             function {
                 """
-                input[0] = false
+                input[0] = true
                 """
             }
         }
@@ -73,9 +61,8 @@ nextflow_function {
         }
     }
 
-    test("Test Function dashedLine") {
-
-        function "dashedLine"
+    test("Test Function with logColours") {
+        function "logColours"
 
         when {
             function {
@@ -93,14 +80,13 @@ nextflow_function {
         }
     }
 
-    test("Test Function without logColours") {
-
-        function "logColours"
+    test("Test Function getSingleReport with a single file") {
+        function "getSingleReport"
 
         when {
             function {
                 """
-                input[0] = true
+                input[0] = file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true)
                 """
             }
         }
@@ -108,18 +94,22 @@ nextflow_function {
         then {
             assertAll(
                 { assert function.success },
-                { assert snapshot(function.result).match() }
+                { assert function.result.contains("test.tsv") }
             )
         }
     }
 
-    test("Test Function with logColours") {
-        function "logColours"
+    test("Test Function getSingleReport with multiple files") {
+        function "getSingleReport"
 
         when {
             function {
                 """
-                input[0] = false
+                input[0] = [
+                    file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + '/generic/tsv/network.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + '/generic/tsv/expression.tsv', checkIfExists: true)
+                ]
                 """
             }
         }
@@ -127,7 +117,9 @@ nextflow_function {
         then {
             assertAll(
                 { assert function.success },
-                { assert snapshot(function.result).match() }
+                { assert function.result.contains("test.tsv") },
+                { assert !function.result.contains("network.tsv") },
+                { assert !function.result.contains("expression.tsv") }
             )
         }
     }
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
index 1037232c9..02c670141 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
@@ -17,26 +17,6 @@
         },
         "timestamp": "2024-02-28T12:02:59.729647"
     },
-    "Test Function nfCoreLogo": {
-        "content": [
-            "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n                                        \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m        ___     __   __   __   ___     \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \u001b[0;33m}  {\u001b[0m\n\u001b[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \u001b[0;32m\\`-._,-`-,\u001b[0m\n                                        \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m  nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-28T12:03:10.562934"
-    },
-    "Test Function workflowCitation": {
-        "content": [
-            "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n  https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n  https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n  https://github.com/nextflow_workflow/blob/master/CITATIONS.md"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-28T12:03:07.019761"
-    },
     "Test Function without logColours": {
         "content": [
             {
@@ -95,16 +75,6 @@
         },
         "timestamp": "2024-02-28T12:03:17.969323"
     },
-    "Test Function dashedLine": {
-        "content": [
-            "-\u001b[2m----------------------------------------------------\u001b[0m-"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-28T12:03:14.366181"
-    },
     "Test Function with logColours": {
         "content": [
             {
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
index 842dc432a..8fb301648 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
@@ -42,7 +42,7 @@ nextflow_workflow {
 
             params {
                 test_data   = ''
-                outdir      = 1
+                outdir      = null
             }
 
             workflow {
@@ -94,7 +94,7 @@ nextflow_workflow {
 
             params {
                 test_data   = ''
-                outdir      = 1
+                outdir      = null
             }
 
             workflow {

From 41523f932dfd76f044d1519a5210336128a55cc6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 9 Dec 2024 12:43:53 +0100
Subject: [PATCH 161/887] prettier

---
 .github/workflows/awsfulltest.yml | 1 -
 1 file changed, 1 deletion(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index d1492d8b8..baaa04619 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -54,4 +54,3 @@ jobs:
           path: |
             seqera_platform_action_*.log
             seqera_platform_action_*.json
-

From 55b0562c864ed88347bc6d1005c06f604a0ad4dc Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Mon, 9 Dec 2024 11:49:03 +0000
Subject: [PATCH 162/887] update tests after fixing fasta in github

---
 subworkflows/local/arriba_workflow/main.nf            | 5 ++---
 subworkflows/local/arriba_workflow/tests/main.nf.test | 2 --
 2 files changed, 2 insertions(+), 5 deletions(-)

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 5460e9263..fbffb7707 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -41,7 +41,7 @@ workflow ARRIBA_WORKFLOW {
 
             if ( arriba_fusions ) {
 
-                ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) ) // This should be done in the main scirpt?
+                ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) ) // Should this be done in the main script?
                     .map { meta, reads, fusions -> [ meta, fusions ] }
                 ch_arriba_fusion_fail = ch_dummy_file
 
@@ -76,8 +76,7 @@ workflow ARRIBA_WORKFLOW {
 
             }
 
-        }
-        else {
+        } else {
             // Not sure how this dummy file can be useful
             // If this tool can be skipped, why not just emitting an empty channel?
             ch_arriba_fusions = reads
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
index 1e713fbb1..3fa816ea5 100644
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test
@@ -25,7 +25,6 @@ nextflow_workflow {
                     // FASTA
                     input[0] = Channel.fromPath(
                             "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                            //"/workspace/rnafusion/results/test.fa", checkIfExists: true
                         )
                         .map{ [[id: it.getName() ], it ]}
 
@@ -63,7 +62,6 @@ nextflow_workflow {
                 input[2] =
                     Channel.fromPath(
                         "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                        //"/workspace/rnafusion/results/test.fa", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 

From 55db5b52281e11465a16de7396fbd3731907896a Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Mon, 9 Dec 2024 12:49:05 +0000
Subject: [PATCH 163/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 335b84c35..7883f2c04 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
 - Add nf-test to local subworkflow: `QC_WORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
+- Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
 
 ### Changed
 

From ce331dc51d239b4325c48c5088821d2ffd99f31b Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 9 Dec 2024 13:07:02 +0000
Subject: [PATCH 164/887] docs: udpdate changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 14eb29741..cc3158142 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,7 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
 - Add nf-test to local subworkflow: `QC_WORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
-- Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/572)
+- Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/577)
 
 ### Changed
 

From d7a06eae5d55fd0413a67e680cc0ad3a05c69429 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 9 Dec 2024 13:36:12 +0000
Subject: [PATCH 165/887] test: update nf-test

---
 tests/test_build.nf.test.snap | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 3b5441dc1..16b6c0079 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -34,7 +34,7 @@
                 "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
                 "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
                 "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,0abf61877f65247b15c438d605d85599",
-                "hgnc_complete_set.txt:md5,34f4bd879d972e511fcb0318df9775b6",
+                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
                 "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,744bf505deb50837b15441e808cad345"
             ]
         ],
@@ -42,6 +42,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-04T17:55:45.068780109"
+        "timestamp": "2024-12-09T13:33:28.517098377"
     }
 }
\ No newline at end of file

From df269a91c1fce57e706107c8bc4a8f039f1a9ebc Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Mon, 9 Dec 2024 13:37:33 +0000
Subject: [PATCH 166/887] update test excluding samtools

---
 subworkflows/local/arriba_workflow/main.nf    |  18 ++-
 .../local/arriba_workflow/tests/main.nf.test  | 120 +++++++++++++++++-
 .../arriba_workflow/tests/main.nf.test.snap   |  49 -------
 3 files changed, 131 insertions(+), 56 deletions(-)
 delete mode 100644 subworkflows/local/arriba_workflow/tests/main.nf.test.snap

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index fbffb7707..4e30c7854 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -19,11 +19,12 @@ workflow ARRIBA_WORKFLOW {
         fusioninspector_only            // boolean
         star_ignore_sjdbgtf             // boolean
         seq_center                      // string
-        arriba_fusions                  // path?
-        cram                            // array?
+        arriba_fusions                  // path
+        cram                            // array
 
     main:
-        ch_versions = Channel.empty()
+        ch_versions   = Channel.empty()
+        ch_bam_index  = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (( arriba || all) && !fusioninspector_only) {
@@ -74,6 +75,10 @@ workflow ARRIBA_WORKFLOW {
                 SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_VIEW_FOR_ARRIBA.out.cram)
                 ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions )
 
+                // Join bam and index files
+                SAMTOOLS_SORT_FOR_ARRIBA.out.bam
+                    .join(SAMTOOLS_INDEX_FOR_ARRIBA.out.cram)
+                    .set{ ch_bam_index }
             }
 
         } else {
@@ -87,8 +92,9 @@ workflow ARRIBA_WORKFLOW {
         }
 
     emit:
-        fusions      = ch_arriba_fusions
-        fusions_fail = ch_arriba_fusion_fail
-        versions     = ch_versions
+        fusions      = ch_arriba_fusions        // channel [ meta, path_fusions   ]
+        fusions_fail = ch_arriba_fusion_fail    // channel [ path, fusions_failed ]
+        bam_index    = ch_bam_index             // channel [ meta, bam, index     ]
+        versions     = ch_versions              // channel [ versions             ]
     }
 
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
index 3fa816ea5..9a9514d7c 100644
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test
@@ -14,6 +14,8 @@ nextflow_workflow {
     tag "star/genomegenerate"
     tag "star/align"
 
+
+    // Test #1 Indexing
     test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4") {
 
         setup {
@@ -114,7 +116,7 @@ nextflow_workflow {
                 // arriba_fusions (path)
                 input[13] = null
 
-                // cram (array??)
+                // cram (array)
                 input[14] = [ 'arriba' ]
                 """
             }
@@ -128,4 +130,120 @@ nextflow_workflow {
         }
     }
 
+
+    // TEST #2 WITHOUT INDEXING
+    test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - cram = []") {
+
+        setup {
+            // Create genome index for STAR
+            run("STAR_GENOMEGENERATE") {
+                script "../../../../modules/nf-core/star/genomegenerate/main"
+                process {
+                """
+                    // FASTA
+                    input[0] = Channel.fromPath(
+                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+
+                    // GTF
+                    input[1] = Channel.fromPath(
+                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+                    """
+                }
+            }
+        }
+
+        when {
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [ id: "test_fastqs" ],
+                        [
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
+                        ]
+                    ] )
+
+                // ch_gtf
+                input[1] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_fasta
+                input[2] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_starindex_ref
+                input[3] = STAR_GENOMEGENERATE.out.index
+
+                // ch_arriba_ref_blacklist
+                input[4] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_known_fusions
+                input[5] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_cytobands
+                input[6] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_protein_domains
+                input[7] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // arriba (boolean)
+                input[8] = true
+
+                // all (boolean)
+                input[9] = true
+
+                // fusioninspector_only  (boolean)
+                input[10] = false
+
+                // star_ignore_sjdbgtf (boolean)
+                input[11] = false
+
+                // seq_center (string)
+                input[12] = 'test_center'
+
+                // arriba_fusions (path)
+                input[13] = null
+
+                // cram (array)
+                input[14] = [ ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
 }
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
deleted file mode 100644
index f7b6a8db3..000000000
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,49 +0,0 @@
-{
-    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
-                    ]
-                ],
-                "1": [
-                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
-                ],
-                "2": [
-                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
-                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
-                    "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
-                    "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
-                    "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
-                ],
-                "fusions": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
-                    ]
-                ],
-                "fusions_fail": [
-                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
-                ],
-                "versions": [
-                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
-                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
-                    "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
-                    "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
-                    "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-06T18:40:22.919178866"
-    }
-}
\ No newline at end of file

From 86c299d54f63a9a387ded1e118868b287f1890e7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 9 Dec 2024 15:41:31 +0100
Subject: [PATCH 167/887] update comments

---
 README.md                                | 2 +-
 docs/usage.md                            | 1 -
 modules/local/hgnc/main.nf               | 1 -
 modules/local/starfusion/build/main.nf   | 1 +
 subworkflows/local/build_references.nf   | 2 +-
 subworkflows/local/trim_workflow/main.nf | 3 +--
 workflows/rnafusion.nf                   | 4 +---
 7 files changed, 5 insertions(+), 9 deletions(-)

diff --git a/README.md b/README.md
index aa8734571..809de85f1 100644
--- a/README.md
+++ b/README.md
@@ -31,7 +31,7 @@ In rnafusion the full-sized test includes reference building and fusion detectio
 
 ### Build references
 
-`--references_only` triggers a workflow to ONLY build references, otherwise the references are built with the analysis run:
+`--references_only` triggers a workflow to ONLY build references, otherwise the references are build when the analysis is run:
 
 1. Download gencode fasta and gtf files
 2. Create [STAR](https://github.com/alexdobin/STAR) index
diff --git a/docs/usage.md b/docs/usage.md
index 1b0db1c57..c383df54c 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -397,7 +397,6 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
 - `test`
   - A profile with a complete configuration for automated testing
   - Includes links to test data so needs no other parameters
-  - Needs to run in two steps: with `--references_only` first and then without `--references_only` to run the analysis
   - !!!! Run with `-stub` as all references need to be downloaded otherwise !!!!
 
 ### `-resume`
diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 2aa56038c..aa5c077cc 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -10,7 +10,6 @@ process HGNC_DOWNLOAD {
     output:
     path "hgnc_complete_set.txt"        , emit: hgnc_ref
     path "HGNC-DB-timestamp.txt"        , emit: hgnc_date
-
     path "versions.yml"   , emit: versions
 
 
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 6ac3c6942..436a5e4fd 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -10,6 +10,7 @@ process STARFUSION_BUILD {
 
     output:
     path "*"  , emit: reference
+    path "versions.yml"   , emit: versions
 
     script:
     def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 8685feebc..2399acbdf 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -94,7 +94,7 @@ workflow BUILD_REFERENCES {
         ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
         ch_salmon_index = SALMON_INDEX.out.index
     } else {
-        ch_salmon_index = Channel.fromPath(params.salmon_index)
+        ch_salmon_index = Channel.fromPath({params.salmon_index})
     }
 
     if ((params.starindex || params.all || params.starfusion || params.arriba) &&
diff --git a/subworkflows/local/trim_workflow/main.nf b/subworkflows/local/trim_workflow/main.nf
index d2492116f..845adbaf5 100644
--- a/subworkflows/local/trim_workflow/main.nf
+++ b/subworkflows/local/trim_workflow/main.nf
@@ -7,7 +7,6 @@ workflow TRIM_WORKFLOW {
 
     take:
         reads           // channel [ meta, [ fastq files ] ]
-        adapter_fasta   // channel [ path ]
 
     main:
         ch_versions       = Channel.empty()
@@ -16,7 +15,7 @@ workflow TRIM_WORKFLOW {
         ch_fastqc_trimmed = Channel.empty()
 
         if ( {params.fastp_trim} ) {
-            FASTP(reads, adapter_fasta.ifEmpty( [] ), false, false, false)
+            FASTP(reads, {params.adapter_fasta}.ifEmpty( [] ), false, false, false)
             ch_versions = ch_versions.mix(FASTP.out.versions)
 
             FASTQC_FOR_FASTP(FASTP.out.reads)
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 523487211..ee0e1e575 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -66,7 +66,6 @@ workflow RNAFUSION {
     BUILD_REFERENCES()
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
-
     //
     // QC from FASTQ files
     //
@@ -81,8 +80,7 @@ workflow RNAFUSION {
     // Trimming
     //
     TRIM_WORKFLOW (
-        ch_samplesheet,
-        ch_adapter_fastp,
+        ch_samplesheet
     )
     ch_reads = TRIM_WORKFLOW.out.ch_reads_all
     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)

From aecc7ef189c360c9944005838401d9c54b212efe Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Mon, 9 Dec 2024 18:27:07 +0000
Subject: [PATCH 168/887] update test to include cram files

---
 subworkflows/local/arriba_workflow/main.nf    |  10 +-
 .../local/arriba_workflow/tests/main.nf.test  |   4 +-
 .../arriba_workflow/tests/main.nf.test.snap   | 114 ++++++++++++++++++
 tests/test_build.nf.test.snap                 |   4 +-
 tests/test_cosmic.nf.test.snap                |   0
 workflows/rnafusion.nf                        |  14 +--
 6 files changed, 129 insertions(+), 17 deletions(-)
 create mode 100644 subworkflows/local/arriba_workflow/tests/main.nf.test.snap
 create mode 100644 tests/test_cosmic.nf.test.snap

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 4e30c7854..28a0a0420 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -24,7 +24,7 @@ workflow ARRIBA_WORKFLOW {
 
     main:
         ch_versions   = Channel.empty()
-        ch_bam_index  = Channel.empty()
+        ch_cram_index = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (( arriba || all) && !fusioninspector_only) {
@@ -75,10 +75,8 @@ workflow ARRIBA_WORKFLOW {
                 SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_VIEW_FOR_ARRIBA.out.cram)
                 ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions )
 
-                // Join bam and index files
-                SAMTOOLS_SORT_FOR_ARRIBA.out.bam
-                    .join(SAMTOOLS_INDEX_FOR_ARRIBA.out.cram)
-                    .set{ ch_bam_index }
+                // Join cram and index files
+                ch_cram_index = SAMTOOLS_VIEW_FOR_ARRIBA.out.cram.join(SAMTOOLS_INDEX_FOR_ARRIBA.out.crai)
             }
 
         } else {
@@ -94,7 +92,7 @@ workflow ARRIBA_WORKFLOW {
     emit:
         fusions      = ch_arriba_fusions        // channel [ meta, path_fusions   ]
         fusions_fail = ch_arriba_fusion_fail    // channel [ path, fusions_failed ]
-        bam_index    = ch_bam_index             // channel [ meta, bam, index     ]
+        cram_index   = ch_cram_index            // channel [ meta, cram, crai     ]
         versions     = ch_versions              // channel [ versions             ]
     }
 
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
index 9a9514d7c..96786a5b0 100644
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test
@@ -21,7 +21,7 @@ nextflow_workflow {
         setup {
             // Create genome index for STAR
             run("STAR_GENOMEGENERATE") {
-                script "../../../../modules/nf-core/star/genomegenerate/main"
+                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
                 process {
                 """
                     // FASTA
@@ -137,7 +137,7 @@ nextflow_workflow {
         setup {
             // Create genome index for STAR
             run("STAR_GENOMEGENERATE") {
-                script "../../../../modules/nf-core/star/genomegenerate/main"
+                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
                 process {
                 """
                     // FASTA
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..3aa777e80
--- /dev/null
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
@@ -0,0 +1,114 @@
+{
+    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.tsv"
+                    ]
+                ],
+                "1": [
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.discarded.tsv"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/test_fastqs_star_for_arriba_sorted.cram",
+                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/test_fastqs_star_for_arriba_sorted.cram.crai"
+                    ]
+                ],
+                "3": [
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/5b/18880ae27c2497bddad57c4b7ba766/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/48/58af6204cc601a1a0e6e826a117f31/versions.yml"
+                ],
+                "cram_index": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/test_fastqs_star_for_arriba_sorted.cram",
+                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/test_fastqs_star_for_arriba_sorted.cram.crai"
+                    ]
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.tsv"
+                    ]
+                ],
+                "fusions_fail": [
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.discarded.tsv"
+                ],
+                "versions": [
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/5b/18880ae27c2497bddad57c4b7ba766/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/versions.yml",
+                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/48/58af6204cc601a1a0e6e826a117f31/versions.yml"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-09T18:02:58.142697295"
+    },
+    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - cram = []": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
+                    ]
+                ],
+                "1": [
+                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
+                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9"
+                ],
+                "cram_index": [
+                    
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test_fastqs"
+                        },
+                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
+                    ]
+                ],
+                "fusions_fail": [
+                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
+                ],
+                "versions": [
+                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
+                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-09T18:15:28.965848873"
+    }
+}
\ No newline at end of file
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 3b5441dc1..28c648187 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -34,7 +34,7 @@
                 "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
                 "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
                 "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,0abf61877f65247b15c438d605d85599",
-                "hgnc_complete_set.txt:md5,34f4bd879d972e511fcb0318df9775b6",
+                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
                 "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,744bf505deb50837b15441e808cad345"
             ]
         ],
@@ -42,6 +42,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-04T17:55:45.068780109"
+        "timestamp": "2024-12-09T18:20:09.914393513"
     }
 }
\ No newline at end of file
diff --git a/tests/test_cosmic.nf.test.snap b/tests/test_cosmic.nf.test.snap
new file mode 100644
index 000000000..e69de29bb
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index a457a1e3c..0908b30c3 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -94,13 +94,13 @@ workflow RNAFUSION {
         ch_arriba_ref_known_fusions,
         ch_arriba_ref_cytobands,
         ch_arriba_ref_protein_domains,
-        params.arriba                   // boolean
-        params.all                      // boolean
-        params.fusioninspector_only     // boolean
-        params.star_ignore_sjdbgtf,
-        params.seq_center ?: '',
-        params.arriba_fusions,          // path
-        params.cram                     // array?
+        params.arriba,                   // boolean
+        params.all,                      // boolean
+        params.fusioninspector_only,     // boolean
+        params.star_ignore_sjdbgtf,      // boolean
+        params.seq_center ?: '',         // string
+        params.arriba_fusions,           // path
+        params.cram                      // array
     )
     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
 

From 9d87a377b35816d4a1ae14246ebc25458464cf1b Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Mon, 9 Dec 2024 18:58:19 +0000
Subject: [PATCH 169/887] remove original script

---
 subworkflows/local/arriba_workflow.nf.bkp | 64 -----------------------
 1 file changed, 64 deletions(-)
 delete mode 100644 subworkflows/local/arriba_workflow.nf.bkp

diff --git a/subworkflows/local/arriba_workflow.nf.bkp b/subworkflows/local/arriba_workflow.nf.bkp
deleted file mode 100644
index ccc888231..000000000
--- a/subworkflows/local/arriba_workflow.nf.bkp
+++ /dev/null
@@ -1,64 +0,0 @@
-include { ARRIBA_ARRIBA }                               from '../../modules/nf-core/arriba/arriba/main'
-include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_ARRIBA}  from '../../modules/nf-core/samtools/index/main'
-include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA}    from '../../modules/nf-core/samtools/sort/main'
-include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_ARRIBA}    from '../../modules/nf-core/samtools/view/main'
-include { STAR_ALIGN as STAR_FOR_ARRIBA }               from '../../modules/nf-core/star/align/main'
-
-workflow ARRIBA_WORKFLOW {
-    take:
-        reads
-        ch_gtf
-        ch_fasta
-        ch_starindex_ref
-        ch_arriba_ref_blacklist
-        ch_arriba_ref_known_fusions
-        ch_arriba_ref_protein_domains
-
-    main:
-        ch_versions = Channel.empty()
-        ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
-
-        if ((params.arriba || params.all) && !params.fusioninspector_only) {
-
-            STAR_FOR_ARRIBA( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
-            ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
-
-            if (params.arriba_fusions) {
-                ch_arriba_fusions = reads.combine( Channel.value( file( params.arriba_fusions, checkIfExists: true ) ) )
-                    .map { meta, reads, fusions -> [ meta, fusions ] }
-                ch_arriba_fusion_fail = ch_dummy_file
-            } else {
-                ARRIBA_ARRIBA ( STAR_FOR_ARRIBA.out.bam, ch_fasta, ch_gtf, ch_arriba_ref_blacklist, ch_arriba_ref_known_fusions, [[],[]], ch_arriba_ref_protein_domains )
-                ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
-
-                ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
-                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ meta, file -> return file}
-            }
-
-            if (params.cram.contains('arriba') ){
-
-                SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam, ch_fasta)
-                ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions )
-
-                SAMTOOLS_VIEW_FOR_ARRIBA(SAMTOOLS_SORT_FOR_ARRIBA.out.bam.map { meta, bam -> [ meta, bam, [] ] }, ch_fasta, [])
-                ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_ARRIBA.out.versions )
-
-                SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_VIEW_FOR_ARRIBA.out.cram)
-                ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions )
-
-            }
-
-        }
-        else {
-            ch_arriba_fusions       = reads.combine(Channel.value( file(ch_dummy_file, checkIfExists:true ) ) )
-                                        .map { meta, reads, fusions -> [ meta, fusions ] }
-
-            ch_arriba_fusion_fail   = ch_dummy_file
-        }
-
-    emit:
-        fusions         = ch_arriba_fusions
-        fusions_fail    = ch_arriba_fusion_fail
-        versions        = ch_versions
-    }
-

From 3a45cbbcdf5081372ed98aa2a10b8783725418a9 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Mon, 9 Dec 2024 19:03:43 +0000
Subject: [PATCH 170/887] fix changelog

---
 CHANGELOG.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 923901134..5a7ecea74 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,7 +22,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/577)
 - Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
 
-
 ### Changed
 
 - Updated modules and migrated non-specific modules to nf-core/modules [#484](https://github.com/nf-core/rnafusion/pull/484)

From 032583a93135193e38d6ce761a6d31dc989b1f05 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 01:05:43 +0000
Subject: [PATCH 171/887] fix: fix container versions

---
 modules/local/starfusion/build/environment.yml | 9 +++++----
 modules/local/starfusion/build/main.nf         | 4 ++--
 2 files changed, 7 insertions(+), 6 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 06a9828af..3c0f4ac53 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -4,7 +4,8 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::star-fusion=1.7.0
-  - bioconda::trinity=2.15.2
-  - bioconda::samtools=1.21
-  - bioconda::star=2.7.11b
+  - bioconda::minimap2
+  - bioconda::samtools
+  - bioconda::star
+  - bioconda::star-fusion=1.14.0
+  - bioconda::trinity
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 6ac3c6942..202dae023 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -2,7 +2,7 @@ process STARFUSION_BUILD {
     tag 'star-fusion'
 
     conda "${moduleDir}/environment.yml"
-    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
+    container 'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:63e3d21ca68ea531'
 
     input:
     tuple val(meta), path(fasta)
@@ -24,7 +24,7 @@ process STARFUSION_BUILD {
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-    $binPath \\
+    /opt/conda/lib/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --annot_filter_rule AnnotFilterRule.pm \\

From 6dcac4fc79d78df11ae8f1d7a2cbb58725490879 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 01:08:37 +0000
Subject: [PATCH 172/887] test: add nf-test initial draft

---
 .../local/fusioninspector/tests/main.nf.test  | 57 +++++++++++++++++++
 .../local/starfusion/build/tests/main.nf.test | 55 ++++++++++++++++++
 2 files changed, 112 insertions(+)
 create mode 100644 modules/local/fusioninspector/tests/main.nf.test
 create mode 100644 modules/local/starfusion/build/tests/main.nf.test

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
new file mode 100644
index 000000000..0afa34acc
--- /dev/null
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -0,0 +1,57 @@
+nextflow_process {
+
+    name "Test Process FUSIONINSPECTOR"
+    script "../main.nf"
+    process "FUSIONINSPECTOR"
+
+    test("FUSIONINSPECTOR - human") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test_sample' ],
+                    [
+                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_1.fastq.gz"),
+                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_2.fastq.gz")
+                    ],
+                    file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/fusion_targets.A.txt")
+                ]
+                input[1] = file("/path/to/reference/genome_lib")
+                """
+            }
+        }
+
+        then {
+            assert snapshot(process.out).match()
+        }
+
+    }
+
+    test("FUSIONINSPECTOR - human - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test_stub' ],
+                    [
+                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_1.fastq.gz"),
+                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_2.fastq.gz")
+                    ],
+                    file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/fusion_targets.A.txt")
+                ]
+                input[1] = file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh37_gencode_v19_CTAT_lib_Mar012021.STAR_v2.7.11a.plug-n-play.tar.gz")
+                """
+            }
+        }
+
+        then {
+            assert snapshot(process.out).match()
+        }
+
+    }
+
+}
diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
new file mode 100644
index 000000000..ef9aa3931
--- /dev/null
+++ b/modules/local/starfusion/build/tests/main.nf.test
@@ -0,0 +1,55 @@
+nextflow_process {
+
+    name "Test Process STARFUSION_BUILD"
+    script "../main.nf"
+    process "STARFUSION_BUILD"
+
+    test("STARFUSION_BUILD - human - chr4") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'fasta' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa")
+                ]
+                input[1] = [
+                    [ id:'gtf' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf")
+                ]
+                """
+            }
+        }
+
+        then {
+            assert snapshot(process.out).match()
+        }
+
+    }
+
+    test("STARFUSION_BUILD - human - chr4") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'fasta' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa")
+                ]
+                input[1] = [
+                    [ id:'gtf' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf")
+                ]
+                """
+            }
+        }
+
+        then {
+            assert snapshot(process.out).match()
+        }
+
+    }
+
+}

From 35b5d9cb50a489c6c576bc2aa93f7cd5a6991e6c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 11:44:24 +0100
Subject: [PATCH 173/887] fix i/o in channels

---
 conf/modules.config                      |  8 +++----
 subworkflows/local/build_references.nf   |  6 +++---
 subworkflows/local/trim_workflow/main.nf |  2 +-
 workflows/rnafusion.nf                   | 27 +++++-------------------
 4 files changed, 13 insertions(+), 30 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index d20ca0f2d..d021ae36e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -18,10 +18,6 @@ process {
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
-    withName: '.*' {
-            ext.when = { !params.references_only || task.process.contains('BUILD_REFERENCES') }
-        }
-
     withName: 'ARRIBA_ARRIBA' {
         publishDir = [
             path: { "${params.outdir}/arriba" },
@@ -371,4 +367,8 @@ process {
     withName: 'VCF_COLLECT' {
         ext.when = { {!params.fusioninspector_only} && {!params.skip_vcf} }
     }
+
+    withName: '.*' {
+            ext.when = { !params.references_only || task.process.contains('BUILD_REFERENCES') }
+        }
 }
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 2399acbdf..e158f566b 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -71,7 +71,7 @@ workflow BUILD_REFERENCES {
         ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
         GET_RRNA_TRANSCRIPTS(ch_gtf)
         ch_versions = ch_versions.mix(GET_RRNA_TRANSCRIPTS.out.versions)
-        GATK4_BEDTOINTERVALLIST(GET_RRNA_TRANSCRIPTS.out.bed.map { it -> [ [id:it.name], it ] }, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
+        GATK4_BEDTOINTERVALLIST(GET_RRNA_TRANSCRIPTS.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
         ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions)
         ch_rrna_interval = GATK4_BEDTOINTERVALLIST.out.interval_list
     } else {
@@ -153,9 +153,9 @@ workflow BUILD_REFERENCES {
             (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
             !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
         if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
-        FUSIONREPORT_DOWNLOAD(params.cosmic_username, params.cosmic_passwd)
+        FUSIONREPORT_DOWNLOAD()
         ch_versions = ch_versions.mix(FUSIONREPORT_DOWNLOAD.out.versions)
-        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.reference
+        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.mitelman.map{ it.parent }
     } else {
         ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref)
     }
diff --git a/subworkflows/local/trim_workflow/main.nf b/subworkflows/local/trim_workflow/main.nf
index 845adbaf5..512a07b54 100644
--- a/subworkflows/local/trim_workflow/main.nf
+++ b/subworkflows/local/trim_workflow/main.nf
@@ -15,7 +15,7 @@ workflow TRIM_WORKFLOW {
         ch_fastqc_trimmed = Channel.empty()
 
         if ( {params.fastp_trim} ) {
-            FASTP(reads, {params.adapter_fasta}.ifEmpty( [] ), false, false, false)
+            FASTP(reads, {params.adapter_fasta}, false, false, false)
             ch_versions = ch_versions.mix(FASTP.out.versions)
 
             FASTQC_FOR_FASTP(FASTP.out.reads)
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ee0e1e575..cf3585339 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -38,27 +38,10 @@ workflow RNAFUSION {
 
     main:
 
-    ch_starindex_ref              = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
-    ch_starindex_ensembl_ref      = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
-    ch_refflat                    = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
-    ch_rrna_interval              = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
-    ch_adapter_fastp              = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta, checkIfExists: true) : Channel.empty()
-    ch_fusionreport_ref           = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
-    ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
-    ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
-    ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
-    ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
-    ch_hgnc_ref                   = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
-    ch_hgnc_date                  = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
-    ch_fasta                      = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
-    ch_gtf                        = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
-    ch_salmon_index               = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
-    ch_fai                        = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
-
-
     ch_versions = Channel.empty()
     ch_multiqc_files = Channel.empty()
 
+
     //
     // Create references if necessary
     //
@@ -66,6 +49,7 @@ workflow RNAFUSION {
     BUILD_REFERENCES()
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
+
     //
     // QC from FASTQ files
     //
@@ -85,7 +69,6 @@ workflow RNAFUSION {
     ch_reads = TRIM_WORKFLOW.out.ch_reads_all
     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
-
     SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
     ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
     ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
@@ -108,7 +91,7 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
 
 
-//Run STAR fusion
+    //Run STAR fusion
     STARFUSION_WORKFLOW (
         ch_reads,
         BUILD_REFERENCES.out.ch_gtf,
@@ -118,14 +101,14 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
 
 
-//Run fusioncatcher
+    //Run fusioncatcher
     FUSIONCATCHER_WORKFLOW (
         ch_reads
     )
     ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
 
 
-//Run stringtie
+    //Run stringtie
     STRINGTIE_WORKFLOW (
         STARFUSION_WORKFLOW.out.ch_bam_sorted,
         BUILD_REFERENCES.out.ch_gtf

From 24a46103dd3a07ae43f22b094e2c7da943126c29 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 12:36:53 +0100
Subject: [PATCH 174/887] test build removed from ci as build_references
 happens before the run

---
 .github/workflows/ci.yml | 1 -
 conf/test_build.config   | 2 +-
 2 files changed, 1 insertion(+), 2 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 71ff72459..a510d72db 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -39,7 +39,6 @@ jobs:
           - "latest-stable"
         test_profile:
           - "test_stub"
-          - "test_build"
         compute_profile:
           - "docker"
           - "singularity"
diff --git a/conf/test_build.config b/conf/test_build.config
index 616d734f0..260023271 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -15,7 +15,7 @@ params {
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
     // Input data
-    build_references           = true
+    references_only            = true
     input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
     no_cosmic                  = true
     all                        = true

From 94fe6092cf313e650b7f4bed6b6ad041da20f09c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 13:37:56 +0100
Subject: [PATCH 175/887] remove double nested folder

---
 CHANGELOG.md                                |  1 +
 conf/modules.config                         |  2 +-
 modules/local/fusionreport/download/main.nf | 22 ++++++++++-----------
 3 files changed, 13 insertions(+), 12 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 690024b2c..b7b8659cc 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
+- Remove double nested folder introduced in [#572](https://github.com/nf-core/rnafusion/pull/577), []()
 
 ### Fixed
 
diff --git a/conf/modules.config b/conf/modules.config
index abe0e79d4..264d1c87e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -114,7 +114,7 @@ process {
         ext.args         = { {params.no_cosmic} ? "--no-cosmic" : " --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         ext.args2        = { params.qiagen ? "--qiagen" : "" }
         publishDir = [
-            path: { "${params.genomes_base}/fusion_report_db" },
+            path: { "${params.genomes_base}" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index b19a64462..89f631db4 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -6,17 +6,17 @@ process FUSIONREPORT_DOWNLOAD {
     container "docker.io/clinicalgenomics/fusion-report:3.1.0"
 
     output:
-    tuple val(meta), path("fusionreport_dbs"), emit: fusionreport_db
+    tuple val(meta), path("fusion_report_db"), emit: fusionreport_ref
     path "versions.yml"                      , emit: versions
 
     script:
-    meta = [id: 'fusionreport_dbs']
+    meta = [id: 'fusion_report_db']
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
     """
     fusion_report download $args ./
-    mkdir fusionreport_dbs
-    mv *.txt *.log *.db fusionreport_dbs
+    mkdir fusion_report_db
+    mv *.txt *.log *.db fusion_report_db/
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -25,14 +25,14 @@ process FUSIONREPORT_DOWNLOAD {
     """
 
     stub:
-    meta = [id: 'fusionreport_dbs']
+    meta = [id: 'fusion_report_db']
     """
-    mkdir fusionreport_dbs
-    touch fusionreport_dbs/cosmic.db
-    touch fusionreport_dbs/fusiongdb2.db
-    touch fusionreport_dbs/mitelman.db
-    touch fusionreport_dbs/DB-timestamp.txt
-    touch fusionreport_dbs/fusion_report.log
+    mkdir fusion_report_db
+    touch fusion_report_db/cosmic.db
+    touch fusion_report_db/fusiongdb2.db
+    touch fusion_report_db/mitelman.db
+    touch fusion_report_db/DB-timestamp.txt
+    touch fusion_report_db/fusion_report.log
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From e52244904c69d632320322f911c935d396c60fad Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 13:39:19 +0100
Subject: [PATCH 176/887] update changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index b7b8659cc..a2603f3e9 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -27,7 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
-- Remove double nested folder introduced in [#572](https://github.com/nf-core/rnafusion/pull/577), []()
+- Remove double nested folder introduced in [#572](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
 
 ### Fixed
 

From 18ab239ce99002d3bab715c734ce3010b704ec5a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 13:40:24 +0100
Subject: [PATCH 177/887] update changelog

---
 CHANGELOG.md | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a2603f3e9..0371c2067 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,7 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
 - Add nf-test to local subworkflow: `QC_WORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
-- Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/577)
+- Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#577](https://github.com/nf-core/rnafusion/pull/577)
 
 ### Changed
 
@@ -27,7 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
-- Remove double nested folder introduced in [#572](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
+- Remove double nested folder introduced in [#577](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
 
 ### Fixed
 

From 76cf2aaffc1630665ec3ad9e1534549eefaa2002 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 14:20:11 +0100
Subject: [PATCH 178/887] update snapshots, add meta

---
 conf/modules.config                           |   2 +-
 conf/test.config                              |   2 +
 modules/local/fusionreport/detect/main.nf     |   2 +-
 modules/local/fusionreport/download/main.nf   |  20 +-
 subworkflows/local/build_references.nf        |   6 +-
 subworkflows/local/fusionreport_workflow.nf   |   1 -
 .../utils_nfcore_rnafusion_pipeline/main.nf   |   2 +-
 test.xml                                      |   1 +
 tests/test_cosmic.nf.test.snap                |  68 +++
 tests/test_stub.nf.test.snap                  | 459 +++++++++++++++++-
 10 files changed, 540 insertions(+), 23 deletions(-)
 create mode 100644 test.xml
 create mode 100644 tests/test_cosmic.nf.test.snap

diff --git a/conf/modules.config b/conf/modules.config
index d021ae36e..bbbee5c2c 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -111,7 +111,7 @@ process {
         ext.args         = { {params.no_cosmic} ? "--no-cosmic" : " --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         ext.args2        = { params.qiagen ? "--qiagen" : "" }
         publishDir = [
-            path: { "${params.genomes_base}/fusion_report_db" },
+            path: { "${params.genomes_base}" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
diff --git a/conf/test.config b/conf/test.config
index 3cafa252b..1902de0a9 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -16,4 +16,6 @@ params {
 
     // Input data
     input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
+    all                    = true
+    no_cosmic              = true
 }
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 5482d4bf7..56a29ab4c 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -7,7 +7,7 @@ process FUSIONREPORT {
 
     input:
     tuple val(meta), path(reads), path(arriba_fusions), path(starfusion_fusions),  path(fusioncatcher_fusions)
-    path(fusionreport_ref)
+    tuple val(meta2), path(fusionreport_ref)
     val(tools_cutoff)
 
     output:
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index b19a64462..a2c2a96ba 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -6,17 +6,17 @@ process FUSIONREPORT_DOWNLOAD {
     container "docker.io/clinicalgenomics/fusion-report:3.1.0"
 
     output:
-    tuple val(meta), path("fusionreport_dbs"), emit: fusionreport_db
+    tuple val(meta), path("fusion_report_db"), emit: fusionreport_ref
     path "versions.yml"                      , emit: versions
 
     script:
-    meta = [id: 'fusionreport_dbs']
+    meta = [id: 'fusion_report_db']
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
     """
     fusion_report download $args ./
-    mkdir fusionreport_dbs
-    mv *.txt *.log *.db fusionreport_dbs
+    mkdir fusion_report_db
+    mv *.txt *.log *.db fusion_report_db
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -27,12 +27,12 @@ process FUSIONREPORT_DOWNLOAD {
     stub:
     meta = [id: 'fusionreport_dbs']
     """
-    mkdir fusionreport_dbs
-    touch fusionreport_dbs/cosmic.db
-    touch fusionreport_dbs/fusiongdb2.db
-    touch fusionreport_dbs/mitelman.db
-    touch fusionreport_dbs/DB-timestamp.txt
-    touch fusionreport_dbs/fusion_report.log
+    mkdir fusion_report_db
+    touch fusion_report_db/cosmic.db
+    touch fusion_report_db/fusiongdb2.db
+    touch fusion_report_db/mitelman.db
+    touch fusion_report_db/DB-timestamp.txt
+    touch fusion_report_db/fusion_report.log
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index e158f566b..8e9cc4b1e 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -152,12 +152,12 @@ workflow BUILD_REFERENCES {
     if ((params.fusionreport || params.all) &&
             (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
             !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
-        if (!params.cosmic_username || !params.cosmic_passwd) { exit 1, 'COSMIC username and/or password missing' }
+        if (!params.no_cosmic && (!params.cosmic_username || !params.cosmic_passwd)) { exit 1, 'COSMIC username and/or password missing' }
         FUSIONREPORT_DOWNLOAD()
         ch_versions = ch_versions.mix(FUSIONREPORT_DOWNLOAD.out.versions)
-        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.mitelman.map{ it.parent }
+        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
     } else {
-        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref)
+        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
     }
 
     emit:
diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index 5c9bfde13..80fe73378 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -19,7 +19,6 @@ workflow FUSIONREPORT_WORKFLOW {
             .join(arriba_fusions, remainder: true)
             .join(starfusion_fusions, remainder: true)
             .join(fusioncatcher_fusions, remainder: true)
-
             FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
             ch_fusion_list = FUSIONREPORT.out.fusion_list
             ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 4b46c5c02..1f905a44b 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -157,7 +157,7 @@ def validateInputParameters() {
     genomeExistsError()
 
     if (params.no_cosmic) {
-            log.warn("Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD`")
+            log.warn("Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`")
     }
 
 }
diff --git a/test.xml b/test.xml
new file mode 100644
index 000000000..e8f1835bc
--- /dev/null
+++ b/test.xml
@@ -0,0 +1 @@
+<?xml version="1.0" encoding="utf-8"?><testsuites><testsuite name="Test pipeline" time="260.628" tests="2" skipped="0" failures="0" timestamp="2024 12 10 14:13:25"><testcase classname="Test pipeline" name="stub test with fastp trim" time="135.663" status="PASSED"></testcase><testcase classname="Test pipeline" name="stub test no fastp trim" time="124.798" status="PASSED"></testcase></testsuite></testsuites>
\ No newline at end of file
diff --git a/tests/test_cosmic.nf.test.snap b/tests/test_cosmic.nf.test.snap
new file mode 100644
index 000000000..dc3ab947a
--- /dev/null
+++ b/tests/test_cosmic.nf.test.snap
@@ -0,0 +1,68 @@
+{
+    "test cosmic no fastp trim build": {
+        "content": [
+            0,
+            {
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-10T12:42:49.558429"
+    },
+    "test cosmic no fastp trim": {
+        "content": [
+            0,
+            {
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-10T12:42:06.51355"
+    },
+    "test cosmic with fastp trim": {
+        "content": [
+            0,
+            {
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-10T12:41:17.841938"
+    }
+}
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 3b83c2400..febef3c8d 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,22 +1,131 @@
 {
     "stub test no fastp trim": {
         "content": [
-            4,
+            31,
             {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.33
+                },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "fusioncatcher.py 1.35"
+                },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
                 },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": null
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.7.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.7.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
+                "fusioncatcher",
+                "fusioncatcher/human_v46",
+                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -25,28 +134,156 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/fusion_report_db",
+                "references/fusion_report_db/DB-timestamp.txt",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/gffread",
+                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/salmon",
+                "references/salmon/salmon",
+                "references/salmon/salmon/complete_ref_lens.bin",
+                "references/salmon/salmon/ctable.bin",
+                "references/salmon/salmon/ctg_offsets.bin",
+                "references/salmon/salmon/duplicate_clusters.tsv",
+                "references/salmon/salmon/info.json",
+                "references/salmon/salmon/mphf.bin",
+                "references/salmon/salmon/pos.bin",
+                "references/salmon/salmon/pre_indexing.log",
+                "references/salmon/salmon/rank.bin",
+                "references/salmon/salmon/refAccumLengths.bin",
+                "references/salmon/salmon/ref_indexing.log",
+                "references/salmon/salmon/reflengths.bin",
+                "references/salmon/salmon/refseq.bin",
+                "references/salmon/salmon/seq.bin",
+                "references/salmon/salmon/versionInfo.json",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ref_annot.cdna.fa",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json"
+                "salmon/test_meta_info.json",
+                "star_for_arriba",
+                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
+                "star_for_arriba/test.Aligned.unsort.out.bam",
+                "star_for_arriba/test.Chimeric.out.junction",
+                "star_for_arriba/test.Log.final.out",
+                "star_for_arriba/test.Log.out",
+                "star_for_arriba/test.Log.progress.out",
+                "star_for_arriba/test.ReadsPerGene.out.tab",
+                "star_for_arriba/test.SJ.out.tab",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_arriba/test.out.sam",
+                "star_for_arriba/test.sortedByCoord.out.bam",
+                "star_for_arriba/test.tab",
+                "star_for_arriba/test.toTranscriptome.out.bam",
+                "star_for_arriba/test.unmapped_1.fastq.gz",
+                "star_for_arriba/test.unmapped_2.fastq.gz",
+                "star_for_arriba/testXd.out.bam",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
+                "star_for_starfusion/test.tab",
+                "star_for_starfusion/test.toTranscriptome.out.bam",
+                "star_for_starfusion/test.unmapped_1.fastq.gz",
+                "star_for_starfusion/test.unmapped_2.fastq.gz",
+                "star_for_starfusion/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T19:27:00.717876194"
+        "timestamp": "2024-12-10T14:12:48.526275"
     },
     "stub test with fastp trim": {
         "content": [
-            6,
+            31,
             {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
                 "FASTP": {
                     "fastp": "0.23.4"
                 },
@@ -56,14 +293,96 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.33
+                },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "fusioncatcher.py 1.35"
+                },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
                 },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": null
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.7.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.7.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -76,6 +395,12 @@
                 "fastqc_for_fastp",
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
+                "fusioncatcher",
+                "fusioncatcher/human_v46",
+                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -84,22 +409,144 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/fusion_report_db",
+                "references/fusion_report_db/DB-timestamp.txt",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/gffread",
+                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/salmon",
+                "references/salmon/salmon",
+                "references/salmon/salmon/complete_ref_lens.bin",
+                "references/salmon/salmon/ctable.bin",
+                "references/salmon/salmon/ctg_offsets.bin",
+                "references/salmon/salmon/duplicate_clusters.tsv",
+                "references/salmon/salmon/info.json",
+                "references/salmon/salmon/mphf.bin",
+                "references/salmon/salmon/pos.bin",
+                "references/salmon/salmon/pre_indexing.log",
+                "references/salmon/salmon/rank.bin",
+                "references/salmon/salmon/refAccumLengths.bin",
+                "references/salmon/salmon/ref_indexing.log",
+                "references/salmon/salmon/reflengths.bin",
+                "references/salmon/salmon/refseq.bin",
+                "references/salmon/salmon/seq.bin",
+                "references/salmon/salmon/versionInfo.json",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ref_annot.cdna.fa",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json"
+                "salmon/test_meta_info.json",
+                "star_for_arriba",
+                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
+                "star_for_arriba/test.Aligned.unsort.out.bam",
+                "star_for_arriba/test.Chimeric.out.junction",
+                "star_for_arriba/test.Log.final.out",
+                "star_for_arriba/test.Log.out",
+                "star_for_arriba/test.Log.progress.out",
+                "star_for_arriba/test.ReadsPerGene.out.tab",
+                "star_for_arriba/test.SJ.out.tab",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_arriba/test.out.sam",
+                "star_for_arriba/test.sortedByCoord.out.bam",
+                "star_for_arriba/test.tab",
+                "star_for_arriba/test.toTranscriptome.out.bam",
+                "star_for_arriba/test.unmapped_1.fastq.gz",
+                "star_for_arriba/test.unmapped_2.fastq.gz",
+                "star_for_arriba/testXd.out.bam",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
+                "star_for_starfusion/test.tab",
+                "star_for_starfusion/test.toTranscriptome.out.bam",
+                "star_for_starfusion/test.unmapped_1.fastq.gz",
+                "star_for_starfusion/test.unmapped_2.fastq.gz",
+                "star_for_starfusion/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T19:26:43.375635514"
+        "timestamp": "2024-12-10T14:10:38.383967"
     }
 }
\ No newline at end of file

From 56ed001b0fce281ba4375db685969541cc70e7af Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 14:25:59 +0100
Subject: [PATCH 179/887] remove trash files

---
 test.fusionreport.tsv          | 0
 test.fusionreport_filtered.tsv | 0
 test.fusions.csv               | 0
 test.fusions.json              | 0
 test.xml                       | 1 -
 test_fusionreport_index.html   | 0
 6 files changed, 1 deletion(-)
 delete mode 100644 test.fusionreport.tsv
 delete mode 100644 test.fusionreport_filtered.tsv
 delete mode 100644 test.fusions.csv
 delete mode 100644 test.fusions.json
 delete mode 100644 test.xml
 delete mode 100644 test_fusionreport_index.html

diff --git a/test.fusionreport.tsv b/test.fusionreport.tsv
deleted file mode 100644
index e69de29bb..000000000
diff --git a/test.fusionreport_filtered.tsv b/test.fusionreport_filtered.tsv
deleted file mode 100644
index e69de29bb..000000000
diff --git a/test.fusions.csv b/test.fusions.csv
deleted file mode 100644
index e69de29bb..000000000
diff --git a/test.fusions.json b/test.fusions.json
deleted file mode 100644
index e69de29bb..000000000
diff --git a/test.xml b/test.xml
deleted file mode 100644
index e8f1835bc..000000000
--- a/test.xml
+++ /dev/null
@@ -1 +0,0 @@
-<?xml version="1.0" encoding="utf-8"?><testsuites><testsuite name="Test pipeline" time="260.628" tests="2" skipped="0" failures="0" timestamp="2024 12 10 14:13:25"><testcase classname="Test pipeline" name="stub test with fastp trim" time="135.663" status="PASSED"></testcase><testcase classname="Test pipeline" name="stub test no fastp trim" time="124.798" status="PASSED"></testcase></testsuite></testsuites>
\ No newline at end of file
diff --git a/test_fusionreport_index.html b/test_fusionreport_index.html
deleted file mode 100644
index e69de29bb..000000000

From 2e928ed27519168d7ee851a9e593496031d5e104 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 14:59:16 +0100
Subject: [PATCH 180/887] update test

---
 modules/local/fusionreport/detect/tests/main.nf.test      | 5 ++---
 modules/local/fusionreport/detect/tests/main.nf.test.snap | 6 +++---
 modules/local/fusionreport/download/tests/main.nf.test    | 8 ++++----
 .../local/fusionreport/download/tests/main.nf.test.snap   | 8 ++++----
 4 files changed, 13 insertions(+), 14 deletions(-)

diff --git a/modules/local/fusionreport/detect/tests/main.nf.test b/modules/local/fusionreport/detect/tests/main.nf.test
index bd5ab356a..9fc895403 100644
--- a/modules/local/fusionreport/detect/tests/main.nf.test
+++ b/modules/local/fusionreport/detect/tests/main.nf.test
@@ -33,8 +33,7 @@ nextflow_process {
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
                 ]
 
-                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_db
-
+                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
                 input[2] = 1
                 """
             }
@@ -91,7 +90,7 @@ nextflow_process {
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
                 ]
 
-                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_db
+                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
 
                 input[2] = 1
                 """
diff --git a/modules/local/fusionreport/detect/tests/main.nf.test.snap b/modules/local/fusionreport/detect/tests/main.nf.test.snap
index fb37037fa..21d16b61b 100644
--- a/modules/local/fusionreport/detect/tests/main.nf.test.snap
+++ b/modules/local/fusionreport/detect/tests/main.nf.test.snap
@@ -68,7 +68,7 @@
         "content": [
             {
                 "0": [
-                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
+                    "versions.yml:md5,8cabeb934dbeb51399427d83c57bf1b4"
                 ],
                 "1": [
                     [
@@ -173,7 +173,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
+                    "versions.yml:md5,8cabeb934dbeb51399427d83c57bf1b4"
                 ]
             }
         ],
@@ -181,6 +181,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-06T13:12:32.981394201"
+        "timestamp": "2024-12-10T14:55:14.475834"
     }
 }
\ No newline at end of file
diff --git a/modules/local/fusionreport/download/tests/main.nf.test b/modules/local/fusionreport/download/tests/main.nf.test
index dd079587f..35af2a52b 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test
+++ b/modules/local/fusionreport/download/tests/main.nf.test
@@ -19,10 +19,10 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    path(process.out.fusionreport_db[0][1]).resolve("fusiongdb2.db"),
-                    path(process.out.fusionreport_db[0][1]).resolve("mitelman.db"),
-                    path(process.out.fusionreport_db[0][1]).resolve("DB-timestamp.txt").exists(),
-                    path(process.out.fusionreport_db[0][1]).resolve("fusion_report.log").exists(),
+                    path(process.out.fusionreport_ref[0][1]).resolve("fusiongdb2.db"),
+                    path(process.out.fusionreport_ref[0][1]).resolve("mitelman.db"),
+                    path(process.out.fusionreport_ref[0][1]).resolve("DB-timestamp.txt").exists(),
+                    path(process.out.fusionreport_ref[0][1]).resolve("fusion_report.log").exists(),
                     process.out.versions
                     ).match() }
             )
diff --git a/modules/local/fusionreport/download/tests/main.nf.test.snap b/modules/local/fusionreport/download/tests/main.nf.test.snap
index c328f7486..797788065 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test.snap
+++ b/modules/local/fusionreport/download/tests/main.nf.test.snap
@@ -21,7 +21,7 @@
                 "0": [
                     [
                         {
-                            "id": "fusionreport_dbs"
+                            "id": "fusion_report_db"
                         },
                         [
                             "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -35,10 +35,10 @@
                 "1": [
                     "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
                 ],
-                "fusionreport_db": [
+                "fusion_report_db": [
                     [
                         {
-                            "id": "fusionreport_dbs"
+                            "id": "fusion_report_db"
                         },
                         [
                             "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -60,4 +60,4 @@
         },
         "timestamp": "2024-12-05T19:35:49.629287874"
     }
-}
\ No newline at end of file
+}

From 82c1fc01a58dec24c61b8756ad2a530a12b54ae0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 15:12:18 +0100
Subject: [PATCH 181/887] update download snapshot

---
 modules/local/fusionreport/download/tests/main.nf.test.snap | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/fusionreport/download/tests/main.nf.test.snap b/modules/local/fusionreport/download/tests/main.nf.test.snap
index 797788065..722d8c68b 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test.snap
+++ b/modules/local/fusionreport/download/tests/main.nf.test.snap
@@ -35,7 +35,7 @@
                 "1": [
                     "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
                 ],
-                "fusion_report_db": [
+                "fusionreport_ref": [
                     [
                         {
                             "id": "fusion_report_db"
@@ -58,6 +58,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-05T19:35:49.629287874"
+        "timestamp": "2024-12-10T15:05:22.781845"
     }
-}
+}
\ No newline at end of file

From 87f5ff80b4b69f74e9d16a08acfa62845d2856d3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 15:14:22 +0100
Subject: [PATCH 182/887] update detect snapshot

---
 modules/local/fusionreport/detect/tests/main.nf.test.snap | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/fusionreport/detect/tests/main.nf.test.snap b/modules/local/fusionreport/detect/tests/main.nf.test.snap
index 21d16b61b..c20e3ec41 100644
--- a/modules/local/fusionreport/detect/tests/main.nf.test.snap
+++ b/modules/local/fusionreport/detect/tests/main.nf.test.snap
@@ -68,7 +68,7 @@
         "content": [
             {
                 "0": [
-                    "versions.yml:md5,8cabeb934dbeb51399427d83c57bf1b4"
+                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
                 ],
                 "1": [
                     [
@@ -173,7 +173,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,8cabeb934dbeb51399427d83c57bf1b4"
+                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
                 ]
             }
         ],
@@ -181,6 +181,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-10T14:55:14.475834"
+        "timestamp": "2024-12-10T15:13:58.414161"
     }
 }
\ No newline at end of file

From 5c124b015f5fa81593c27b3f1a789f47c92f64d4 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Tue, 10 Dec 2024 14:19:26 +0000
Subject: [PATCH 183/887] restructure module

---
 modules/local/fusioncatcher/detect/main.nf    | 10 +--
 modules/local/fusioncatcher/download/main.nf  |  5 +-
 ...kflow.nf => fusioncatcher_workflow.nf.bkp} |  0
 .../local/fusioncatcher_workflow/main.nf      | 40 ++++++++++++
 .../fusioncatcher_workflow/tests/main.nf.test | 65 +++++++++++++++++++
 .../tests/main.nf.test.snap                   | 25 +++++++
 6 files changed, 138 insertions(+), 7 deletions(-)
 rename subworkflows/local/{fusioncatcher_workflow.nf => fusioncatcher_workflow.nf.bkp} (100%)
 create mode 100644 subworkflows/local/fusioncatcher_workflow/main.nf
 create mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
 create mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 9a0928463..ddbacce1e 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -1,13 +1,13 @@
 process FUSIONCATCHER {
-    tag "$meta.id"
+    tag "$meta.id - $meta2.id"
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
     container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
 
     input:
-    tuple val(meta), path(fasta)
-    path reference
+    tuple val(meta), path(fastqs, stageAs: "input/*")
+    tuple val(meta2), path(reference, stageAs: "reference/*")
 
     output:
     tuple val(meta), path("*.fusioncatcher.fusion-genes.txt")   , optional:true  , emit: fusions
@@ -25,8 +25,8 @@ process FUSIONCATCHER {
     def single_end = meta.single_end ? "--single-end" : ""
     """
     fusioncatcher.py \\
-        -d $reference \\
-        -i $reads \\
+        -d reference \\
+        -i input \\
         -p $task.cpus \\
         -o . \\
         --skip-blat \\
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 65a736077..b0659a153 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -6,8 +6,8 @@ process FUSIONCATCHER_DOWNLOAD {
     container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
 
     output:
-    path "*"                , emit: reference
-    path "versions.yml"     , emit: versions
+    tuple env(meta), path("*"), emit: reference
+    path "versions.yml"       , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -18,6 +18,7 @@ process FUSIONCATCHER_DOWNLOAD {
     def args2 = task.ext.args2 ?: ''
     def human_version = "v102"
     def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
+    def meta = [ id: "human_${human_version}" ]
     """
     if wget --spider "$url" 2>/dev/null; then
         wget $args $url
diff --git a/subworkflows/local/fusioncatcher_workflow.nf b/subworkflows/local/fusioncatcher_workflow.nf.bkp
similarity index 100%
rename from subworkflows/local/fusioncatcher_workflow.nf
rename to subworkflows/local/fusioncatcher_workflow.nf.bkp
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
new file mode 100644
index 000000000..c268545a7
--- /dev/null
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -0,0 +1,40 @@
+include { FUSIONCATCHER } from '../../../modules/local/fusioncatcher/detect/main'
+
+workflow FUSIONCATCHER_WORKFLOW {
+    take:
+        reads                   // channel [ meta, [ fastqs ] ]
+        fusioncatcher_ref       // channel [ meta, path       ]
+        run_fusioncatcher       // boolean
+        all                     // boolean
+        fusioninspector_only    // boolean
+        fusioncatcher_fusions   // path, string
+
+    main:
+        ch_versions = Channel.empty()
+        ch_dummy_file = file("$baseDir/assets/dummy_file_fusioncatcher.txt", checkIfExists: true)
+
+        if (( run_fusioncatcher || all) && !fusioninspector_only ) {
+            if (fusioncatcher_fusions){
+
+                ch_fusioncatcher_fusions = reads.combine(Channel.value(file(fusioncatcher_fusions, checkIfExists:true)))
+                                            .map { meta, reads, fusions -> [ meta, fusions ] }
+            } else {
+
+                FUSIONCATCHER (
+                    reads,
+                    fusioncatcher_ref
+                )
+                ch_fusioncatcher_fusions = FUSIONCATCHER.out.fusions
+                ch_versions = ch_versions.mix(FUSIONCATCHER.out.versions)
+            }
+        }
+        else {
+            ch_fusioncatcher_fusions = reads.combine(Channel.value(file(ch_dummy_file, checkIfExists:true)))
+                                        .map { meta, reads, fusions -> [ meta, fusions ] }
+        }
+
+    emit:
+        fusions  = ch_fusioncatcher_fusions
+        versions = ch_versions
+    }
+
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
new file mode 100644
index 000000000..4d91798ca
--- /dev/null
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -0,0 +1,65 @@
+nextflow_workflow {
+
+    name "Test Subworkflow FUSIONCATCHER_WORKFLOW"
+    script "../main.nf"
+    workflow "FUSIONCATCHER_WORKFLOW"
+    tag "subworkflow"
+    tag "fusioncatcher"
+    tag "fusioncatcher/download"
+    tag "fusioncatcher/detect"
+
+    // Test
+    test("FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4") {
+
+        setup {
+            // Download reference files for fusioncatch
+            run("FUSIONCATCHER_DOWNLOAD") {
+                script "../../../../modules/local/fusioncatcher/download/main.nf"
+                process {
+                """
+                // No arguments required. Human version v102
+                """
+                }
+            }
+        }
+
+        when {
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [ id: "test_fastqs", single_end: false ],
+                        [
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
+                        ]
+                    ] )
+
+                // ch_references
+                input[1] = FUSIONCATCHER_DOWNLOAD.out.reference
+
+                // fusioncatcher (boolean)
+                input[2] = true
+
+                // all (boolean)
+                input[3] = true
+
+                // fusioninspector_only (boolean)
+                input[4] = false
+
+                // fusioncatcher_fusions (string path)
+                input[5] = null
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..19d409a0a
--- /dev/null
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -0,0 +1,25 @@
+{
+    "FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    
+                ],
+                "fusions": [
+                    
+                ],
+                "versions": [
+                    
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-09T20:39:28.49363464"
+    }
+}
\ No newline at end of file

From 23c4014a6a22839223540a7dd98426fb89cd31d7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 15:24:20 +0100
Subject: [PATCH 184/887] use stubs

---
 .github/workflows/awstest.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 78bdb317c..977adb49a 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -26,6 +26,7 @@ jobs:
               "cosmic_username": "${{ secrets.cosmic_username }}",
               "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
               "all": true,
+              "stub": true
             }
           profiles: test,aws_tower
       - uses: actions/upload-artifact@v4

From d52f0a055fb82ae8def0b400c016820af38bf222 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 15:24:42 +0100
Subject: [PATCH 185/887] update changelog, first step

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 690024b2c..1980cd861 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -27,7 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
-
+        ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons" GTF_TO_REFFLAT
 ### Fixed
 
 - Fixed some Nextflow run-commands in the docs [#491](https://github.com/nf-core/rnafusion/pull/491)

From 5409f8c2566342a4d2debe406ef4e19b52231d74 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 15:25:02 +0100
Subject: [PATCH 186/887] update GTF_TO_REFFLAT options, update snapshots

---
 conf/modules.config                          |  1 +
 modules/local/fusioncatcher/build/meta.yml   |  3 +-
 modules/local/fusioncatcher/download/main.nf |  2 +-
 modules/local/starfusion/download/main.nf    |  2 +-
 nextflow.config                              |  3 +-
 tests/test_build.nf.test.snap                | 33 +++-----------------
 tests/test_cosmic.nf.test.snap               | 22 ++++---------
 7 files changed, 15 insertions(+), 51 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index bbbee5c2c..3c3765453 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -144,6 +144,7 @@ process {
     }
 
     withName: 'GTF_TO_REFFLAT' {
+        ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons"
         publishDir = [
             path: { "${params.genomes_base}/gencode" },
             mode: params.publish_dir_mode,
diff --git a/modules/local/fusioncatcher/build/meta.yml b/modules/local/fusioncatcher/build/meta.yml
index 40421a4ee..202be7e17 100644
--- a/modules/local/fusioncatcher/build/meta.yml
+++ b/modules/local/fusioncatcher/build/meta.yml
@@ -1,4 +1,4 @@
-name: fusioncatcher_download
+name: fusioncatcher_build
 description: Build genome for fusioncatcher
 keywords:
   - sort
@@ -19,7 +19,6 @@ output:
   - reference:
       type: directory
       description: Path to fusioncatcher references
-      pattern: "*"
 
 authors:
   - "@praveenraj2018, @rannick"
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 7afdb4c6d..ed97c834a 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -20,7 +20,7 @@ process FUSIONCATCHER_DOWNLOAD {
 
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
-        // TODO: move to my sourceforge
+        // TODO: move to S3
 
     // def url =
     """
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 85bb39c15..6ef1df633 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -8,7 +8,7 @@ process STARFUSION_DOWNLOAD {
     path "ctat_genome_lib_build_dir/*"            , emit: reference
 
 
-    // TODO: move to my sourceforge
+    // TODO: move to S3
     script:
     """
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.10/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
diff --git a/nextflow.config b/nextflow.config
index 31e754979..db9eb1417 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -61,13 +61,12 @@ params {
 
     // Skip steps
     skip_qc                    = false
-    skip_vcf                   = false
     skip_vis                   = false
     skip_vcf                   = false
     skip_salmon_index          = false
 
     // Download references option
-    download_refs              = true
+    download_refs              = false
 
     // Path to references
     fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 16b6c0079..5332634b5 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -2,46 +2,21 @@
     "test_build": {
         "content": [
             [
-                "bedops",
-                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
-                "gffread",
-                "gffread/Homo_sapiens.GRCh38.102.fasta",
                 "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
                 "references",
-                "references/ensembl",
-                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
-                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
-                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
-                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
                 "references/hgnc",
                 "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "rrnatranscripts",
-                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
+                "references/hgnc/hgnc_complete_set.txt"
             ],
             [
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,5bc3ccc76735ae46699a75269f0ea65b",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,092f1bf29fce906ff7b5ece02b4b21c8",
-                "Homo_sapiens.GRCh38.102.fasta:md5,d1f17b045dc60c49f2cc29e30006afc0",
-                "Homo_sapiens.GRCh38.102.chr.gtf:md5,0069687307852c63596b4d2ebdbbaf0c",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d527f3eb6b664020cf4d882b5820056f",
-                "Homo_sapiens.GRCh38.102.genepred:md5,59c577ca4ab033c0ce1feac1e387bcab",
-                "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
-                "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,0abf61877f65247b15c438d605d85599",
-                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,744bf505deb50837b15441e808cad345"
+                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-09T13:33:28.517098377"
+        "timestamp": "2024-12-10T15:09:04.076558"
     }
 }
\ No newline at end of file
diff --git a/tests/test_cosmic.nf.test.snap b/tests/test_cosmic.nf.test.snap
index dc3ab947a..b8e6d4a5f 100644
--- a/tests/test_cosmic.nf.test.snap
+++ b/tests/test_cosmic.nf.test.snap
@@ -24,14 +24,9 @@
     "test cosmic no fastp trim": {
         "content": [
             0,
-            {
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            null,
             [
-                "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+                "pipeline_info"
             ],
             [
                 
@@ -41,19 +36,14 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-10T12:42:06.51355"
+        "timestamp": "2024-12-10T15:09:53.130295"
     },
     "test cosmic with fastp trim": {
         "content": [
             0,
-            {
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            null,
             [
-                "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+                "pipeline_info"
             ],
             [
                 
@@ -63,6 +53,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-10T12:41:17.841938"
+        "timestamp": "2024-12-10T15:09:29.277399"
     }
 }
\ No newline at end of file

From ef5ab96f749a1dd7d6c32f54f39fa395892c7a20 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 10 Dec 2024 16:00:35 +0100
Subject: [PATCH 187/887] update star in snapshot

---
 tests/test_stub.nf.test.snap | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index febef3c8d..0c4a35781 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -80,17 +80,17 @@
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": 2.7.11b,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": 2.7.11b,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": 2.7.11b,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -355,17 +355,17 @@
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": 2.7.11b,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": 2.7.11b,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": 2.7.11b,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -549,4 +549,4 @@
         },
         "timestamp": "2024-12-10T14:10:38.383967"
     }
-}
\ No newline at end of file
+}

From 58aab76ed09c8434b0249531cbca20368c3b0dfb Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 19:11:47 +0000
Subject: [PATCH 188/887] fix: simplify starfusion module

---
 modules/local/starfusion/build/main.nf | 28 +++++++++-----------------
 1 file changed, 10 insertions(+), 18 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 202dae023..4d3c70b6f 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -7,31 +7,23 @@ process STARFUSION_BUILD {
     input:
     tuple val(meta), path(fasta)
     tuple val(meta2), path(gtf)
+    path fusion_annot_lib
+    val dfam_species
 
     output:
-    path "*"  , emit: reference
+    path "ctat_genome_lib_build_dir"  , emit: reference
 
     script:
-    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
+    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/opt/conda/lib/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
+    if (dfam_species != "human" && dfam_species != "mouse") {
+        error "Invalid species for --dfam_db. Only 'human' or 'mouse' are accepted. Provided: ${dfam_species}"
+    }
     """
-    export TMPDIR=/tmp
-    wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
-    wget https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz -O CTAT_HumanFusionLib_Mar2021.dat.gz --no-check-certificate
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
-    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-    /opt/conda/lib/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
+    prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
-        --annot_filter_rule AnnotFilterRule.pm \\
-        --fusion_annot_lib CTAT_HumanFusionLib_Mar2021.dat.gz \\
-        --pfam_db Pfam-A.hmm \\
-        --dfam_db homo_sapiens_dfam.hmm \\
-        --max_readlength $params.read_length \\
+        --dfam_db ${dfam_species} \\
+        --fusion_annot_lib $fusion_annot_lib \\
         --CPU $task.cpus
 
     cat <<-END_VERSIONS > versions.yml

From 7ff8be18d1466fe07ea8eb74a11f9b56c0e1d014 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 19:15:57 +0000
Subject: [PATCH 189/887] tests: update nf-test starfusion

---
 .../local/starfusion/build/tests/main.nf.test | 28 ++++++++++++-------
 1 file changed, 18 insertions(+), 10 deletions(-)

diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
index ef9aa3931..75993c004 100644
--- a/modules/local/starfusion/build/tests/main.nf.test
+++ b/modules/local/starfusion/build/tests/main.nf.test
@@ -4,19 +4,23 @@ nextflow_process {
     script "../main.nf"
     process "STARFUSION_BUILD"
 
-    test("STARFUSION_BUILD - human - chr4") {
+    test("STARFUSION_BUILD - human - minigenome") {
 
         when {
             process {
                 """
                 input[0] = [
-                    [ id:'fasta' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa")
+                    [ id:'minigenome fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
                 ]
                 input[1] = [
-                    [ id:'gtf' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf")
+                    [ id:'minigenome gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
                 ]
+
+                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input [3] = "human"
+
                 """
             }
         }
@@ -27,7 +31,7 @@ nextflow_process {
 
     }
 
-    test("STARFUSION_BUILD - human - chr4") {
+    test("STARFUSION_BUILD - human - minigenome - stub") {
 
         options "-stub"
 
@@ -35,13 +39,17 @@ nextflow_process {
             process {
                 """
                 input[0] = [
-                    [ id:'fasta' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa")
+                    [ id:'minigenome fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
                 ]
                 input[1] = [
-                    [ id:'gtf' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf")
+                    [ id:'minigenome gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
                 ]
+
+                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input [3] = "human"
+
                 """
             }
         }

From 0770939ea7f410f511aa01955bc6e07ea44f76e2 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 21:19:53 +0000
Subject: [PATCH 190/887] refactor: add stubs and ext.args

---
 modules/local/starfusion/build/main.nf | 42 ++++++++++++++++++++++++--
 1 file changed, 39 insertions(+), 3 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 4d3c70b6f..129436480 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -1,5 +1,6 @@
 process STARFUSION_BUILD {
-    tag 'star-fusion'
+    tag "$meta.id"
+    label 'process_high'
 
     conda "${moduleDir}/environment.yml"
     container 'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:63e3d21ca68ea531'
@@ -14,16 +15,18 @@ process STARFUSION_BUILD {
     path "ctat_genome_lib_build_dir"  , emit: reference
 
     script:
-    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/opt/conda/lib/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
     if (dfam_species != "human" && dfam_species != "mouse") {
         error "Invalid species for --dfam_db. Only 'human' or 'mouse' are accepted. Provided: ${dfam_species}"
     }
+    def args = task.ext.args ?: ''
     """
     prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --dfam_db ${dfam_species} \\
+        --pfam_db current \\
         --fusion_annot_lib $fusion_annot_lib \\
+        ${args} \\
         --CPU $task.cpus
 
     cat <<-END_VERSIONS > versions.yml
@@ -35,7 +38,40 @@ process STARFUSION_BUILD {
     stub:
     """
     mkdir ctat_genome_lib_build_dir
-    touch ref_annot.cdna.fa
+    touch ctat_genome_lib_build_dir/AnnotFilterRule.pm
+    touch ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
+    touch ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
+    touch ctat_genome_lib_build_dir/ref_genome.fa.mm2
+    touch ctat_genome_lib_build_dir/ref_genome.fa.ntf
+    touch ctat_genome_lib_build_dir/blast_pairs.dat.gz
+    touch ctat_genome_lib_build_dir/ref_annot.cdna.fa
+    touch ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
+    touch ctat_genome_lib_build_dir/ref_genome.fa.ndb
+    touch ctat_genome_lib_build_dir/ref_genome.fa.nto
+    touch ctat_genome_lib_build_dir/blast_pairs.idx
+    touch ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx
+    touch ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed
+    touch ctat_genome_lib_build_dir/ref_genome.fa.nhr
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx
+    touch ctat_genome_lib_build_dir/__chkpts
+    touch ctat_genome_lib_build_dir/ref_annot.cds
+    touch ctat_genome_lib_build_dir/ref_annot.pep
+    touch ctat_genome_lib_build_dir/ref_genome.fa.nin
+    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat
+    touch ctat_genome_lib_build_dir/fusion_annot_lib.gz
+    touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
+    touch ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
+    touch ctat_genome_lib_build_dir/ref_genome.fa.njs
+    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
+    touch ctat_genome_lib_build_dir/fusion_annot_lib.idx
+    touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
+    touch ctat_genome_lib_build_dir/ref_genome.fa
+    touch ctat_genome_lib_build_dir/ref_genome.fa.not
+    touch ctat_genome_lib_build_dir/trans.blast.dat.gz
+    touch ctat_genome_lib_build_dir/pfam_domains.dbm
+    touch ctat_genome_lib_build_dir/ref_annot.gtf
+    touch ctat_genome_lib_build_dir/ref_genome.fa.fai
+    touch ctat_genome_lib_build_dir/ref_genome.fa.nsq
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From 5b0b9e108798bf0804aa791986bbbac9e6c3bf40 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 21:42:15 +0000
Subject: [PATCH 191/887] docs: update meta.yml

---
 modules/local/starfusion/build/meta.yml | 11 +++++++++--
 1 file changed, 9 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/meta.yml b/modules/local/starfusion/build/meta.yml
index c87b251b1..d7f65a0b9 100644
--- a/modules/local/starfusion/build/meta.yml
+++ b/modules/local/starfusion/build/meta.yml
@@ -1,7 +1,7 @@
-name: starfusion_downloadgenome
+name: starfusion_build
 description: Download STAR-fusion genome resource required to run STAR-Fusion caller
 keywords:
-  - downoad
+  - download
 tools:
   - star-fusion:
       description: Fusion calling algorithm for RNAseq data
@@ -20,6 +20,13 @@ input:
       type: file
       description: genome gtf file
       pattern: "*.{gtf}"
+  - fusion_annot_lib:
+      type: file
+      description: Fusion annotation library (key/val pairs, tab-delimited).
+      pattern: "*.dat.gz"
+  - dfam_species:
+      type: string
+      description: DNA transposable element database (Dfam.hmm), required for repeat masking. Only 'human' or 'mouse' are accepted (will automatically pull the resources from dfam).
 
 output:
   - reference:

From 572e8df646ff4f7bf01eece487a3363dca6720c3 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 22:13:14 +0000
Subject: [PATCH 192/887] test: update snapshots

---
 modules/local/starfusion/build/main.nf        |   8 +-
 .../starfusion/build/tests/main.nf.test.snap  | 260 ++++++++++++++++++
 2 files changed, 264 insertions(+), 4 deletions(-)
 create mode 100644 modules/local/starfusion/build/tests/main.nf.test.snap

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 129436480..80b529118 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -39,11 +39,11 @@ process STARFUSION_BUILD {
     """
     mkdir ctat_genome_lib_build_dir
     touch ctat_genome_lib_build_dir/AnnotFilterRule.pm
-    touch ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
+    gzip -c /dev/null > ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
     touch ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
     touch ctat_genome_lib_build_dir/ref_genome.fa.mm2
     touch ctat_genome_lib_build_dir/ref_genome.fa.ntf
-    touch ctat_genome_lib_build_dir/blast_pairs.dat.gz
+    gzip -c /dev/null > ctat_genome_lib_build_dir/blast_pairs.dat.gz
     touch ctat_genome_lib_build_dir/ref_annot.cdna.fa
     touch ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
     touch ctat_genome_lib_build_dir/ref_genome.fa.ndb
@@ -58,7 +58,7 @@ process STARFUSION_BUILD {
     touch ctat_genome_lib_build_dir/ref_annot.pep
     touch ctat_genome_lib_build_dir/ref_genome.fa.nin
     touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat
-    touch ctat_genome_lib_build_dir/fusion_annot_lib.gz
+    gzip -c /dev/null > ctat_genome_lib_build_dir/fusion_annot_lib.gz
     touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
     touch ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
     touch ctat_genome_lib_build_dir/ref_genome.fa.njs
@@ -67,7 +67,7 @@ process STARFUSION_BUILD {
     touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
     touch ctat_genome_lib_build_dir/ref_genome.fa
     touch ctat_genome_lib_build_dir/ref_genome.fa.not
-    touch ctat_genome_lib_build_dir/trans.blast.dat.gz
+    gzip -c /dev/null > ctat_genome_lib_build_dir/trans.blast.dat.gz
     touch ctat_genome_lib_build_dir/pfam_domains.dbm
     touch ctat_genome_lib_build_dir/ref_annot.gtf
     touch ctat_genome_lib_build_dir/ref_genome.fa.fai
diff --git a/modules/local/starfusion/build/tests/main.nf.test.snap b/modules/local/starfusion/build/tests/main.nf.test.snap
new file mode 100644
index 000000000..5e5e99acf
--- /dev/null
+++ b/modules/local/starfusion/build/tests/main.nf.test.snap
@@ -0,0 +1,260 @@
+{
+    "STARFUSION_BUILD - human - minigenome": {
+        "content": [
+            {
+                "0": [
+                    [
+                        "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+                        "PFAM.domtblout.dat.gz:md5,6d8ba244e68072be23895ec22c8a8ce4",
+                        [
+                            "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ],
+                        "blast_pairs.dat.gz:md5,06d08c55cfa099ccb56d312ca0970729",
+                        "blast_pairs.idx:md5,884cc8c9886409f7ec0fdf8586ffb78d",
+                        "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                        "fusion_annot_lib.idx:md5,39ab4cd5eab589c5fce9b4bfed82c729",
+                        "pfam_domains.dbm:md5,04c75694f8e36461b43d1c693fdf3414",
+                        "ref_annot.cdna.fa:md5,fe801b66cfab298c575971401e93c18c",
+                        "ref_annot.cdna.fa.idx:md5,3c19e33d5424174f1a3de1f21f6746ff",
+                        "ref_annot.cds:md5,4376fb48bd8bf47f13854b3f6bba8297",
+                        "ref_annot.cdsplus.fa:md5,71e98a5b5d8cf371ec4b5db32c19120e",
+                        "ref_annot.cdsplus.fa.idx:md5,8cb47913bfb0d73d3f55cd5ceafa8bfe",
+                        "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                        "ref_annot.gtf.gene_spans:md5,f178dd54a1f81174dfd203e212937e63",
+                        "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+                        "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                        "ref_annot.pep:md5,fe3f58b430d99c06c816521ac439c4df",
+                        "ref_annot.prot_info.dbm:md5,6983b8dccd3e3f920566fab403a15090",
+                        "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+                        "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                        "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+                        "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+                        "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                        "ref_genome.fa.nin:md5,167a13425cf4aac970ae936bd43cbd43",
+                        "ref_genome.fa.njs:md5,7350b9e036410da25ed2c0d717649221",
+                        "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+                        "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+                        "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+                        "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+                        [
+                            "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                            "Log.out:md5,944cceca8093617ab4ece780628459f5",
+                            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+                            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+                            "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                            "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                            "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                            "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                            "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                            "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                            "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                            "genomeParameters.txt:md5,aabd07882af60af4bba0438a475e4e1a",
+                            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+                            "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                            "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                            "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2"
+                        ],
+                        "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                        "trans.blast.align_coords.align_coords.dbm:md5,6000e63ebabe57652cfa8858ef6cc484",
+                        "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
+                    ]
+                ],
+                "reference": [
+                    [
+                        "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+                        "PFAM.domtblout.dat.gz:md5,6d8ba244e68072be23895ec22c8a8ce4",
+                        [
+                            "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ],
+                        "blast_pairs.dat.gz:md5,06d08c55cfa099ccb56d312ca0970729",
+                        "blast_pairs.idx:md5,884cc8c9886409f7ec0fdf8586ffb78d",
+                        "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                        "fusion_annot_lib.idx:md5,39ab4cd5eab589c5fce9b4bfed82c729",
+                        "pfam_domains.dbm:md5,04c75694f8e36461b43d1c693fdf3414",
+                        "ref_annot.cdna.fa:md5,fe801b66cfab298c575971401e93c18c",
+                        "ref_annot.cdna.fa.idx:md5,3c19e33d5424174f1a3de1f21f6746ff",
+                        "ref_annot.cds:md5,4376fb48bd8bf47f13854b3f6bba8297",
+                        "ref_annot.cdsplus.fa:md5,71e98a5b5d8cf371ec4b5db32c19120e",
+                        "ref_annot.cdsplus.fa.idx:md5,8cb47913bfb0d73d3f55cd5ceafa8bfe",
+                        "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                        "ref_annot.gtf.gene_spans:md5,f178dd54a1f81174dfd203e212937e63",
+                        "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+                        "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                        "ref_annot.pep:md5,fe3f58b430d99c06c816521ac439c4df",
+                        "ref_annot.prot_info.dbm:md5,6983b8dccd3e3f920566fab403a15090",
+                        "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+                        "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                        "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+                        "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+                        "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                        "ref_genome.fa.nin:md5,167a13425cf4aac970ae936bd43cbd43",
+                        "ref_genome.fa.njs:md5,7350b9e036410da25ed2c0d717649221",
+                        "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+                        "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+                        "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+                        "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+                        [
+                            "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                            "Log.out:md5,944cceca8093617ab4ece780628459f5",
+                            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+                            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+                            "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                            "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                            "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                            "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                            "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                            "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                            "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                            "genomeParameters.txt:md5,aabd07882af60af4bba0438a475e4e1a",
+                            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+                            "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                            "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                            "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2"
+                        ],
+                        "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                        "trans.blast.align_coords.align_coords.dbm:md5,6000e63ebabe57652cfa8858ef6cc484",
+                        "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-10T22:11:39.460127957"
+    },
+    "STARFUSION_BUILD - human - minigenome - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reference": [
+                    [
+                        "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-10T22:12:14.50896742"
+    }
+}
\ No newline at end of file

From 5f6857e8d865c8bf60aa7f9015065769337bd297 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 10 Dec 2024 22:18:32 +0000
Subject: [PATCH 193/887] refactor: revert add nf-test initial draft

---
 .../local/fusioninspector/tests/main.nf.test  | 57 -------------------
 1 file changed, 57 deletions(-)
 delete mode 100644 modules/local/fusioninspector/tests/main.nf.test

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
deleted file mode 100644
index 0afa34acc..000000000
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ /dev/null
@@ -1,57 +0,0 @@
-nextflow_process {
-
-    name "Test Process FUSIONINSPECTOR"
-    script "../main.nf"
-    process "FUSIONINSPECTOR"
-
-    test("FUSIONINSPECTOR - human") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test_sample' ],
-                    [
-                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_1.fastq.gz"),
-                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_2.fastq.gz")
-                    ],
-                    file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/fusion_targets.A.txt")
-                ]
-                input[1] = file("/path/to/reference/genome_lib")
-                """
-            }
-        }
-
-        then {
-            assert snapshot(process.out).match()
-        }
-
-    }
-
-    test("FUSIONINSPECTOR - human - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test_stub' ],
-                    [
-                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_1.fastq.gz"),
-                        file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/test.reads_2.fastq.gz")
-                    ],
-                    file("https://raw.githubusercontent.com/FusionInspector/FusionInspector/master/test/fusion_targets.A.txt")
-                ]
-                input[1] = file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh37_gencode_v19_CTAT_lib_Mar012021.STAR_v2.7.11a.plug-n-play.tar.gz")
-                """
-            }
-        }
-
-        then {
-            assert snapshot(process.out).match()
-        }
-
-    }
-
-}

From 117884c783977964fe3ee9196ebbc44944f2eaa3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 11 Dec 2024 10:39:08 +0100
Subject: [PATCH 194/887] add human_gencode_filter to starfusion build

---
 CHANGELOG.md                                 |   3 +-
 modules/local/fusioncatcher/download/main.nf |   1 -
 modules/local/starfusion/build/main.nf       |   1 +
 subworkflows/local/arriba_workflow.nf        |   2 +-
 test.xml                                     | 240 +++++++++++++++++++
 5 files changed, 244 insertions(+), 3 deletions(-)
 create mode 100644 test.xml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1980cd861..a1444c08d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -27,7 +27,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated to nf-core/tools 3.0.2 [#504](https://github.com/nf-core/rnafusion/pull/504)
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
-        ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons" GTF_TO_REFFLAT
+  ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons" GTF_TO_REFFLAT
+
 ### Fixed
 
 - Fixed some Nextflow run-commands in the docs [#491](https://github.com/nf-core/rnafusion/pull/491)
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index ed97c834a..03c0a1f3c 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -19,7 +19,6 @@ process FUSIONCATCHER_DOWNLOAD {
     script:
 
     def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
         // TODO: move to S3
 
     // def url =
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 436a5e4fd..10485ac00 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -33,6 +33,7 @@ process STARFUSION_BUILD {
         --pfam_db Pfam-A.hmm \\
         --dfam_db homo_sapiens_dfam.hmm \\
         --max_readlength $params.read_length \\
+        --human_gencode_filter \\
         --CPU $task.cpus
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 5d8446783..5575f5c0d 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -52,7 +52,7 @@ workflow ARRIBA_WORKFLOW {
         }
         else {
             ch_arriba_fusions       = reads.combine(Channel.value( file(ch_dummy_file, checkIfExists:true ) ) )
-                                        .map { meta, reads, fusions -> [ meta, fusions ] }
+                                        .map { it -> [ it[0], it[2] ] }
 
             ch_arriba_fusion_fail   = ch_dummy_file
         }
diff --git a/test.xml b/test.xml
new file mode 100644
index 000000000..2aed29e11
--- /dev/null
+++ b/test.xml
@@ -0,0 +1,240 @@
+<?xml version="1.0" encoding="utf-8"?><testsuites><testsuite name="Test pipeline" time="410.367" tests="2" skipped="0" failures="2" timestamp="2024 12 11 10:14:41"><testcase classname="Test pipeline" name="stub test with fastp trim" time="213.214" status="FAILED"><failure message="Assertion failed: 
+
+1 of 2 assertions failed
+">Nextflow stdout:
+
+N E X T F L O W  ~  version 24.10.2
+Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [elated_pasteur] DSL2 - revision: dc78081240
+
+------------------------------------------------------
+                                        ,--./,-.
+        ___     __   __   __   ___     /,-._.--~'
+  |\ | |__  __ /  ` /  \ |__) |__         }  {
+  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
+                                        `._,._,'
+  nf-core/rnafusion 4.0.0dev
+------------------------------------------------------
+Input/output options
+  input                             : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv
+  outdir                            : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output
+  genomes_base                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references
+  genome_gencode_version            : 46
+  starfusion_build                  : true
+  all                               : true
+  arriba_ref_blacklist              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
+  arriba_ref_cytobands              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
+  arriba_ref_known_fusions          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
+  arriba_ref_protein_domains        : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
+  gencode_ref                       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode
+  fusioncatcher_limitSjdbInsertNsj  : 2000000
+  fusioncatcher_ref                 : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusioncatcher/human_v46
+  fusioncatcher_ref_stub_check      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt
+  fusioninspector_limitSjdbInsertNsj: 1000000
+  fusionreport_ref                  : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusion_report_db
+  fusionreport_ref_stub_check       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusion_report_db/mitelman.db
+  hgnc_ref                          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/hgnc/hgnc_complete_set.txt
+  hgnc_date                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/hgnc/HGNC-DB-timestamp.txt
+  salmon_index                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/salmon/salmon
+  salmon_index_stub_check           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/salmon/salmon/complete_ref_lens.bin
+  starfusion_ref                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/starfusion/ctat_genome_lib_build_dir
+  starfusion_ref_stub_check         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/starfusion/Pfam-A.hmm
+  starindex_ref                     : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/star
+  starindex_ref_stub_check          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/star/star/Genome
+  tools_cutoff                      : 1
+
+Read trimming options
+  fastp_trim                        : true
+  adapter_fasta                     : []
+
+Alignment compression options
+  cram                              : []
+
+Reference genome options
+  fasta                             : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa
+  fai                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa.fai
+  genome                            : GRCh38
+  gtf                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46.gtf
+  refflat                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46.gtf.refflat
+  rrna_intervals                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46.interval_list
+  no_cosmic                         : true
+
+Institutional config options
+  config_profile_name               : Test profile
+  config_profile_description        : Minimal test dataset to check pipeline function
+
+Core Nextflow options
+  runName                           : elated_pasteur
+  containerEngine                   : docker
+  launchDir                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0
+  workDir                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/work
+  projectDir                        : /Users/annick.renevey/Projects/rnafusion
+  userName                          : annick.renevey
+  profile                           : test,docker
+  configFiles                       : 
+
+!! Only displaying parameters that differ from the pipeline defaults !!
+------------------------------------------------------
+* The nf-core framework
+    https://doi.org/10.1038/s41587-020-0439-x
+
+* Software dependencies
+    https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md
+
+WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected.
+HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.
+
+WARN: Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`
+[13/a75f51] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONCATCHER_BUILD (fusioncatcher_build)
+[78/f27e8a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc)
+[c9/d109b6] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:ARRIBA_DOWNLOAD (arriba)
+[1b/cb8505] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GENCODE_DOWNLOAD (gencode_download)
+[61/738ba4] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONREPORT_DOWNLOAD (fusionreport)
+[6c/ccb83a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FASTQC (test)
+[52/593378] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTP (test)
+[18/18b5f3] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
+[38/36e8dd] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
+[b3/7aad17] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GET_RRNA_TRANSCRIPTS (get_rrna_bed)
+[53/e4d111] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STAR_GENOMEGENERATE (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
+[37/d0930c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GFFREAD (Homo_sapiens.GRCh38.46.gtf)
+[26/aa9b36] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT (Homo_sapiens.GRCh38.46.gtf)
+[18/66b9c3] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STARFUSION_BUILD (star-fusion)
+[05/6e1246] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTQC_FOR_FASTP (test)
+[83/52bcc1] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONCATCHER_WORKFLOW:FUSIONCATCHER (test)
+[5b/c0d7bc] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST (Homo_sapiens.GRCh38.46.gtf)
+[24/c5d0b9] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STAR_FOR_STARFUSION (test)
+[43/8be8df] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:STAR_FOR_ARRIBA (test)
+[f9/87735b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SALMON_INDEX (Homo_sapiens.GRCh38.46.gtf.fasta)
+[b4/64a1cd] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTRNASEQMETRICS (test)
+[cc/99a46b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTINSERTSIZEMETRICS (test)
+[0b/18a022] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION (test)
+[aa/000cc3] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:SAMTOOLS_INDEX_FOR_STARFUSION (test)
+[3d/4dfbba] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_STRINGTIE (test)
+[10/6bc883] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:GATK4_MARKDUPLICATES (test)
+[74/397e36] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:ARRIBA_ARRIBA (test)
+[df/483739] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:SALMON_QUANT (test)
+[92/376a4b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_MERGE (1)
+[b6/26ca21] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONREPORT_WORKFLOW:FUSIONREPORT (test)
+[51/2177ee] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:MULTIQC
+-[nf-core/rnafusion] Pipeline completed successfully-
+Nextflow stderr:
+
+</failure></testcase><testcase classname="Test pipeline" name="stub test no fastp trim" time="197.086" status="FAILED"><failure message="Assertion failed: 
+
+1 of 2 assertions failed
+">Nextflow stdout:
+
+N E X T F L O W  ~  version 24.10.2
+Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [naughty_becquerel] DSL2 - revision: dc78081240
+
+------------------------------------------------------
+                                        ,--./,-.
+        ___     __   __   __   ___     /,-._.--~'
+  |\ | |__  __ /  ` /  \ |__) |__         }  {
+  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
+                                        `._,._,'
+  nf-core/rnafusion 4.0.0dev
+------------------------------------------------------
+Input/output options
+  input                             : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv
+  outdir                            : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output
+  genomes_base                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references
+  genome_gencode_version            : 46
+  starfusion_build                  : true
+  all                               : true
+  arriba_ref_blacklist              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
+  arriba_ref_cytobands              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
+  arriba_ref_known_fusions          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
+  arriba_ref_protein_domains        : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
+  gencode_ref                       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode
+  fusioncatcher_limitSjdbInsertNsj  : 2000000
+  fusioncatcher_ref                 : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusioncatcher/human_v46
+  fusioncatcher_ref_stub_check      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt
+  fusioninspector_limitSjdbInsertNsj: 1000000
+  fusionreport_ref                  : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusion_report_db
+  fusionreport_ref_stub_check       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusion_report_db/mitelman.db
+  hgnc_ref                          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/hgnc/hgnc_complete_set.txt
+  hgnc_date                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/hgnc/HGNC-DB-timestamp.txt
+  salmon_index                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/salmon/salmon
+  salmon_index_stub_check           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/salmon/salmon/complete_ref_lens.bin
+  starfusion_ref                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/starfusion/ctat_genome_lib_build_dir
+  starfusion_ref_stub_check         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/starfusion/Pfam-A.hmm
+  starindex_ref                     : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/star
+  starindex_ref_stub_check          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/star/star/Genome
+  tools_cutoff                      : 1
+
+Read trimming options
+  adapter_fasta                     : []
+
+Alignment compression options
+  cram                              : []
+
+Reference genome options
+  fasta                             : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa
+  fai                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa.fai
+  genome                            : GRCh38
+  gtf                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46.gtf
+  refflat                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46.gtf.refflat
+  rrna_intervals                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46.interval_list
+  no_cosmic                         : true
+
+Institutional config options
+  config_profile_name               : Test profile
+  config_profile_description        : Minimal test dataset to check pipeline function
+
+Core Nextflow options
+  runName                           : naughty_becquerel
+  containerEngine                   : docker
+  launchDir                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1
+  workDir                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/work
+  projectDir                        : /Users/annick.renevey/Projects/rnafusion
+  userName                          : annick.renevey
+  profile                           : test,docker
+  configFiles                       : 
+
+!! Only displaying parameters that differ from the pipeline defaults !!
+------------------------------------------------------
+* The nf-core framework
+    https://doi.org/10.1038/s41587-020-0439-x
+
+* Software dependencies
+    https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md
+
+WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected.
+HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.
+
+WARN: Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`
+[8d/ee20bb] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc)
+[d6/69323a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GENCODE_DOWNLOAD (gencode_download)
+[b9/5c89ae] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONREPORT_DOWNLOAD (fusionreport)
+[43/355279] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONCATCHER_BUILD (fusioncatcher_build)
+[94/cf96bf] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:ARRIBA_DOWNLOAD (arriba)
+[e4/d33580] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
+[db/fa3dd4] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
+[90/118532] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FASTQC (test)
+[b9/6593b4] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTP (test)
+[b4/28611c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT (Homo_sapiens.GRCh38.46.gtf)
+[d0/9b767c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GET_RRNA_TRANSCRIPTS (get_rrna_bed)
+[c2/fd36fd] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GFFREAD (Homo_sapiens.GRCh38.46.gtf)
+[76/b9b243] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STAR_GENOMEGENERATE (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
+[d6/350166] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STARFUSION_BUILD (star-fusion)
+[89/c38359] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTQC_FOR_FASTP (test)
+[92/431af8] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONCATCHER_WORKFLOW:FUSIONCATCHER (test)
+[43/c4079e] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST (Homo_sapiens.GRCh38.46.gtf)
+[e6/3c5e19] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SALMON_INDEX (Homo_sapiens.GRCh38.46.gtf.fasta)
+[71/f9cbec] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:STAR_FOR_ARRIBA (test)
+[96/59d525] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STAR_FOR_STARFUSION (test)
+[99/6d4d21] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:SALMON_QUANT (test)
+[9c/fd7ac5] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:ARRIBA_ARRIBA (test)
+[29/6b167b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_STRINGTIE (test)
+[39/c6c23e] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION (test)
+[35/474e2f] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:SAMTOOLS_INDEX_FOR_STARFUSION (test)
+[66/b0ff6a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:GATK4_MARKDUPLICATES (test)
+[63/a246b6] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTRNASEQMETRICS (test)
+[41/50018c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTINSERTSIZEMETRICS (test)
+[8c/96974e] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_MERGE (1)
+[fe/e581e9] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONREPORT_WORKFLOW:FUSIONREPORT (test)
+[99/47d296] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:MULTIQC
+-[nf-core/rnafusion] Pipeline completed successfully-
+Nextflow stderr:
+
+</failure></testcase></testsuite></testsuites>
\ No newline at end of file

From 3788788d1b26dd07dd9cfb85c8cb1091f6630f8e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 11 Dec 2024 10:44:52 +0100
Subject: [PATCH 195/887] remove test.xml

---
 test.xml | 240 -------------------------------------------------------
 1 file changed, 240 deletions(-)
 delete mode 100644 test.xml

diff --git a/test.xml b/test.xml
deleted file mode 100644
index 2aed29e11..000000000
--- a/test.xml
+++ /dev/null
@@ -1,240 +0,0 @@
-<?xml version="1.0" encoding="utf-8"?><testsuites><testsuite name="Test pipeline" time="410.367" tests="2" skipped="0" failures="2" timestamp="2024 12 11 10:14:41"><testcase classname="Test pipeline" name="stub test with fastp trim" time="213.214" status="FAILED"><failure message="Assertion failed: 
-
-1 of 2 assertions failed
-">Nextflow stdout:
-
-N E X T F L O W  ~  version 24.10.2
-Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [elated_pasteur] DSL2 - revision: dc78081240
-
-------------------------------------------------------
-                                        ,--./,-.
-        ___     __   __   __   ___     /,-._.--~'
-  |\ | |__  __ /  ` /  \ |__) |__         }  {
-  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
-                                        `._,._,'
-  nf-core/rnafusion 4.0.0dev
-------------------------------------------------------
-Input/output options
-  input                             : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv
-  outdir                            : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output
-  genomes_base                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references
-  genome_gencode_version            : 46
-  starfusion_build                  : true
-  all                               : true
-  arriba_ref_blacklist              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
-  arriba_ref_cytobands              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
-  arriba_ref_known_fusions          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
-  arriba_ref_protein_domains        : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
-  gencode_ref                       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode
-  fusioncatcher_limitSjdbInsertNsj  : 2000000
-  fusioncatcher_ref                 : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusioncatcher/human_v46
-  fusioncatcher_ref_stub_check      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt
-  fusioninspector_limitSjdbInsertNsj: 1000000
-  fusionreport_ref                  : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusion_report_db
-  fusionreport_ref_stub_check       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/fusion_report_db/mitelman.db
-  hgnc_ref                          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/hgnc/hgnc_complete_set.txt
-  hgnc_date                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/hgnc/HGNC-DB-timestamp.txt
-  salmon_index                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/salmon/salmon
-  salmon_index_stub_check           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/salmon/salmon/complete_ref_lens.bin
-  starfusion_ref                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/starfusion/ctat_genome_lib_build_dir
-  starfusion_ref_stub_check         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/starfusion/Pfam-A.hmm
-  starindex_ref                     : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/star
-  starindex_ref_stub_check          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/star/star/Genome
-  tools_cutoff                      : 1
-
-Read trimming options
-  fastp_trim                        : true
-  adapter_fasta                     : []
-
-Alignment compression options
-  cram                              : []
-
-Reference genome options
-  fasta                             : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa
-  fai                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa.fai
-  genome                            : GRCh38
-  gtf                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46.gtf
-  refflat                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46.gtf.refflat
-  rrna_intervals                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/output/references/gencode/Homo_sapiens_GRCh38_46.interval_list
-  no_cosmic                         : true
-
-Institutional config options
-  config_profile_name               : Test profile
-  config_profile_description        : Minimal test dataset to check pipeline function
-
-Core Nextflow options
-  runName                           : elated_pasteur
-  containerEngine                   : docker
-  launchDir                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0
-  workDir                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/acc69f529568702c4fabd8a30cf1fcb0/work
-  projectDir                        : /Users/annick.renevey/Projects/rnafusion
-  userName                          : annick.renevey
-  profile                           : test,docker
-  configFiles                       : 
-
-!! Only displaying parameters that differ from the pipeline defaults !!
-------------------------------------------------------
-* The nf-core framework
-    https://doi.org/10.1038/s41587-020-0439-x
-
-* Software dependencies
-    https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md
-
-WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected.
-HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.
-
-WARN: Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`
-[13/a75f51] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONCATCHER_BUILD (fusioncatcher_build)
-[78/f27e8a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc)
-[c9/d109b6] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:ARRIBA_DOWNLOAD (arriba)
-[1b/cb8505] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GENCODE_DOWNLOAD (gencode_download)
-[61/738ba4] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONREPORT_DOWNLOAD (fusionreport)
-[6c/ccb83a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FASTQC (test)
-[52/593378] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTP (test)
-[18/18b5f3] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
-[38/36e8dd] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
-[b3/7aad17] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GET_RRNA_TRANSCRIPTS (get_rrna_bed)
-[53/e4d111] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STAR_GENOMEGENERATE (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
-[37/d0930c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GFFREAD (Homo_sapiens.GRCh38.46.gtf)
-[26/aa9b36] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT (Homo_sapiens.GRCh38.46.gtf)
-[18/66b9c3] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STARFUSION_BUILD (star-fusion)
-[05/6e1246] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTQC_FOR_FASTP (test)
-[83/52bcc1] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONCATCHER_WORKFLOW:FUSIONCATCHER (test)
-[5b/c0d7bc] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST (Homo_sapiens.GRCh38.46.gtf)
-[24/c5d0b9] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STAR_FOR_STARFUSION (test)
-[43/8be8df] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:STAR_FOR_ARRIBA (test)
-[f9/87735b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SALMON_INDEX (Homo_sapiens.GRCh38.46.gtf.fasta)
-[b4/64a1cd] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTRNASEQMETRICS (test)
-[cc/99a46b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTINSERTSIZEMETRICS (test)
-[0b/18a022] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION (test)
-[aa/000cc3] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:SAMTOOLS_INDEX_FOR_STARFUSION (test)
-[3d/4dfbba] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_STRINGTIE (test)
-[10/6bc883] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:GATK4_MARKDUPLICATES (test)
-[74/397e36] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:ARRIBA_ARRIBA (test)
-[df/483739] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:SALMON_QUANT (test)
-[92/376a4b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_MERGE (1)
-[b6/26ca21] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONREPORT_WORKFLOW:FUSIONREPORT (test)
-[51/2177ee] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:MULTIQC
--[nf-core/rnafusion] Pipeline completed successfully-
-Nextflow stderr:
-
-</failure></testcase><testcase classname="Test pipeline" name="stub test no fastp trim" time="197.086" status="FAILED"><failure message="Assertion failed: 
-
-1 of 2 assertions failed
-">Nextflow stdout:
-
-N E X T F L O W  ~  version 24.10.2
-Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [naughty_becquerel] DSL2 - revision: dc78081240
-
-------------------------------------------------------
-                                        ,--./,-.
-        ___     __   __   __   ___     /,-._.--~'
-  |\ | |__  __ /  ` /  \ |__) |__         }  {
-  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
-                                        `._,._,'
-  nf-core/rnafusion 4.0.0dev
-------------------------------------------------------
-Input/output options
-  input                             : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv
-  outdir                            : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output
-  genomes_base                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references
-  genome_gencode_version            : 46
-  starfusion_build                  : true
-  all                               : true
-  arriba_ref_blacklist              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
-  arriba_ref_cytobands              : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
-  arriba_ref_known_fusions          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
-  arriba_ref_protein_domains        : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
-  gencode_ref                       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode
-  fusioncatcher_limitSjdbInsertNsj  : 2000000
-  fusioncatcher_ref                 : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusioncatcher/human_v46
-  fusioncatcher_ref_stub_check      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt
-  fusioninspector_limitSjdbInsertNsj: 1000000
-  fusionreport_ref                  : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusion_report_db
-  fusionreport_ref_stub_check       : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/fusion_report_db/mitelman.db
-  hgnc_ref                          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/hgnc/hgnc_complete_set.txt
-  hgnc_date                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/hgnc/HGNC-DB-timestamp.txt
-  salmon_index                      : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/salmon/salmon
-  salmon_index_stub_check           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/salmon/salmon/complete_ref_lens.bin
-  starfusion_ref                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/starfusion/ctat_genome_lib_build_dir
-  starfusion_ref_stub_check         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/starfusion/Pfam-A.hmm
-  starindex_ref                     : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/star
-  starindex_ref_stub_check          : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/star/star/Genome
-  tools_cutoff                      : 1
-
-Read trimming options
-  adapter_fasta                     : []
-
-Alignment compression options
-  cram                              : []
-
-Reference genome options
-  fasta                             : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa
-  fai                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa.fai
-  genome                            : GRCh38
-  gtf                               : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46.gtf
-  refflat                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46.gtf.refflat
-  rrna_intervals                    : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/output/references/gencode/Homo_sapiens_GRCh38_46.interval_list
-  no_cosmic                         : true
-
-Institutional config options
-  config_profile_name               : Test profile
-  config_profile_description        : Minimal test dataset to check pipeline function
-
-Core Nextflow options
-  runName                           : naughty_becquerel
-  containerEngine                   : docker
-  launchDir                         : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1
-  workDir                           : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/5d4f02e50caba01d5f317164e187f7c1/work
-  projectDir                        : /Users/annick.renevey/Projects/rnafusion
-  userName                          : annick.renevey
-  profile                           : test,docker
-  configFiles                       : 
-
-!! Only displaying parameters that differ from the pipeline defaults !!
-------------------------------------------------------
-* The nf-core framework
-    https://doi.org/10.1038/s41587-020-0439-x
-
-* Software dependencies
-    https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md
-
-WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected.
-HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.
-
-WARN: Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`
-[8d/ee20bb] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc)
-[d6/69323a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GENCODE_DOWNLOAD (gencode_download)
-[b9/5c89ae] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONREPORT_DOWNLOAD (fusionreport)
-[43/355279] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:FUSIONCATCHER_BUILD (fusioncatcher_build)
-[94/cf96bf] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:ARRIBA_DOWNLOAD (arriba)
-[e4/d33580] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
-[db/fa3dd4] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
-[90/118532] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FASTQC (test)
-[b9/6593b4] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTP (test)
-[b4/28611c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT (Homo_sapiens.GRCh38.46.gtf)
-[d0/9b767c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GET_RRNA_TRANSCRIPTS (get_rrna_bed)
-[c2/fd36fd] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GFFREAD (Homo_sapiens.GRCh38.46.gtf)
-[76/b9b243] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STAR_GENOMEGENERATE (Homo_sapiens.GRCh38.46_dna_primary_assembly.fa)
-[d6/350166] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:STARFUSION_BUILD (star-fusion)
-[89/c38359] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:TRIM_WORKFLOW:FASTQC_FOR_FASTP (test)
-[92/431af8] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONCATCHER_WORKFLOW:FUSIONCATCHER (test)
-[43/c4079e] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST (Homo_sapiens.GRCh38.46.gtf)
-[e6/3c5e19] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:BUILD_REFERENCES:SALMON_INDEX (Homo_sapiens.GRCh38.46.gtf.fasta)
-[71/f9cbec] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:STAR_FOR_ARRIBA (test)
-[96/59d525] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STAR_FOR_STARFUSION (test)
-[99/6d4d21] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:SALMON_QUANT (test)
-[9c/fd7ac5] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:ARRIBA_WORKFLOW:ARRIBA_ARRIBA (test)
-[29/6b167b] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_STRINGTIE (test)
-[39/c6c23e] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION (test)
-[35/474e2f] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:SAMTOOLS_INDEX_FOR_STARFUSION (test)
-[66/b0ff6a] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:GATK4_MARKDUPLICATES (test)
-[63/a246b6] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTRNASEQMETRICS (test)
-[41/50018c] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:QC_WORKFLOW:PICARD_COLLECTINSERTSIZEMETRICS (test)
-[8c/96974e] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:STRINGTIE_WORKFLOW:STRINGTIE_MERGE (1)
-[fe/e581e9] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:FUSIONREPORT_WORKFLOW:FUSIONREPORT (test)
-[99/47d296] Submitted process &gt; NFCORE_RNAFUSION:RNAFUSION:MULTIQC
--[nf-core/rnafusion] Pipeline completed successfully-
-Nextflow stderr:
-
-</failure></testcase></testsuite></testsuites>
\ No newline at end of file

From 8e9be7cd6f83b175bdc15ef0a3fc8f9c89a33897 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 11 Dec 2024 12:45:38 +0100
Subject: [PATCH 196/887] update snap

---
 tests/test_stub.nf.test.snap | 18 +++++++++---------
 1 file changed, 9 insertions(+), 9 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 0c4a35781..6cefb329b 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -80,17 +80,17 @@
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": 2.7.11b,
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": 2.7.11b,
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": 2.7.11b,
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -272,7 +272,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-10T14:12:48.526275"
+        "timestamp": "2024-12-11T12:43:59.534889"
     },
     "stub test with fastp trim": {
         "content": [
@@ -355,17 +355,17 @@
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": 2.7.11b,
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": 2.7.11b,
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": 2.7.11b,
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -547,6 +547,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-10T14:10:38.383967"
+        "timestamp": "2024-12-11T12:40:22.066494"
     }
-}
+}
\ No newline at end of file

From 5cdf95aabc8c45e9687889558db88eddb603c925 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 11 Dec 2024 13:23:10 +0100
Subject: [PATCH 197/887] try updating snapshot again

---
 tests/test_stub.nf.test.snap | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 6cefb329b..83c76dd72 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -80,17 +80,17 @@
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -355,17 +355,17 @@
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -549,4 +549,4 @@
         },
         "timestamp": "2024-12-11T12:40:22.066494"
     }
-}
\ No newline at end of file
+}

From c257c62c7af34ddf0b3621fff1e0a9440cc57c20 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 11 Dec 2024 13:23:28 +0000
Subject: [PATCH 198/887] fix: add versions to dependencies

---
 modules/local/starfusion/build/environment.yml | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 3c0f4ac53..d02ab2d69 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -4,8 +4,8 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::minimap2
-  - bioconda::samtools
-  - bioconda::star
+  - bioconda::minimap2=2.28
+  - bioconda::samtools=1.6
+  - bioconda::star=2.7.11a
   - bioconda::star-fusion=1.14.0
-  - bioconda::trinity
+  - bioconda::trinity=2.8.5

From f2df932841d056dfdc05821822e10848f0144267 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 11 Dec 2024 13:42:58 +0000
Subject: [PATCH 199/887] feat: add fusion annot lib as an external param

---
 nextflow.config                                            | 1 +
 nextflow_schema.json                                       | 5 +++++
 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 4 ++++
 workflows/build_references.nf                              | 4 +++-
 4 files changed, 13 insertions(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index d436182c4..2d148cf9e 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -30,6 +30,7 @@ params {
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
+    fusion_annot_lib           = null
 
     // Genomes options
     fasta          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 017a19fb8..05df1956b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -362,6 +362,11 @@
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "Avoid using Cosmic DB (for example in clinical case applications where a paid license applies."
+                },
+                "fusion_annot_lib": {
+                    "type": "string",
+                    "description": "Path to Fusion Annotation Library to be used in STARFUSION_BUILD.",
+                    "fa_icon": "far fa-file-code"
                 }
             }
         },
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index a4aaf9c4b..ad97686a3 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -161,6 +161,10 @@ def validateInputParameters() {
             log.warn("No cosmic credentials were provided. Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD`")
     }
 
+    if (params.starfusion_build && !params.fusion_annot_lib) {
+            error("No fusion annotation library provided. `STARFUSION_BUILD` is unable to run.")
+    }
+
 }
 
 //
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 4886a7bb7..037e87055 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -68,7 +68,9 @@ workflow BUILD_REFERENCES {
 
     if (params.starfusion || params.all) {
         if (params.starfusion_build){
-            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
+            val_species = Channel.value("human")
+            ch_fusion_annot_lib = params.fusion_annot_lib
+            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf, ch_fusion_annot_lib, val_species)
         } else {
             STARFUSION_DOWNLOAD()
         }

From 9dc94cbd968bf00b72501bc07b57b5c6d316a91c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 11 Dec 2024 15:19:43 +0000
Subject: [PATCH 200/887] docs: add param type and where to get it

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 2d148cf9e..32ccc1d0e 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -30,7 +30,7 @@ params {
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
-    fusion_annot_lib           = null
+    fusion_annot_lib           = null       // path to  dat.gz CTAT genome lib (e.g. https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v44_CTAT_lib_Oct292023.plug-n-play.tar.gz)
 
     // Genomes options
     fasta          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"

From d2a7bd4e75746efeb8c193a8836792648e7fc7e7 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 11 Dec 2024 15:34:31 +0000
Subject: [PATCH 201/887] test: run starfusion in test_build

---
 conf/test_build.config | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/conf/test_build.config b/conf/test_build.config
index 616d734f0..2449ee9a7 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -21,4 +21,7 @@ params {
     all                        = true
 
     skip_salmon_index          = true
+    starfusion_build           = true
+    fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+
 }

From cf71e6265f86a966f12a5487e5dfa32b988bda66 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 11 Dec 2024 15:34:58 +0000
Subject: [PATCH 202/887] refactor: add metamap to output

---
 modules/local/starfusion/build/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 80b529118..f3014ca93 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -12,7 +12,7 @@ process STARFUSION_BUILD {
     val dfam_species
 
     output:
-    path "ctat_genome_lib_build_dir"  , emit: reference
+    tuple val(meta), path("ctat_genome_lib_build_dir"), emit: reference
 
     script:
     if (dfam_species != "human" && dfam_species != "mouse") {

From ef36aedf24c9206bfe24763ee5bb4cf01aa97a69 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 11 Dec 2024 16:00:41 +0000
Subject: [PATCH 203/887] test: update tests

---
 .../local/starfusion/build/tests/main.nf.test |  77 +++-
 .../starfusion/build/tests/main.nf.test.snap  | 388 +++++++-----------
 2 files changed, 231 insertions(+), 234 deletions(-)

diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
index 75993c004..8eb1b6f66 100644
--- a/modules/local/starfusion/build/tests/main.nf.test
+++ b/modules/local/starfusion/build/tests/main.nf.test
@@ -26,7 +26,82 @@ nextflow_process {
         }
 
         then {
-            assert snapshot(process.out).match()
+            assert snapshot(
+                    path(process.out.reference[0][1]).resolve("AnnotFilterRule.pm"),
+                    path(process.out.reference[0][1]).resolve("blast_pairs.dat.gz").exists(),
+                    path(process.out.reference[0][1]).resolve("blast_pairs.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("__chkpts/annotfiltrule_cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/blast_pairs.idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/cp_gene_blast_pairs.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/cp_pfam_dat.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/cp_ref_annot_cdna.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/fusion_annot_lib.cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/_fusion_annot_lib.idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/index_pfam_hits.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/index_ref_annot_cdna.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/makeblastdb.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/mm2_genome_idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/mm2.splice_bed.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/_prot_info_db.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.gene_spans.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.mini.sortu.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome_fai.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome.fa.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.index.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/validate_ctat_genome_lib.ok"),
+                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.gz"),
+                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("pfam_domains.dbm").exists(),
+                    path(process.out.reference[0][1]).resolve("PFAM.domtblout.dat.gz").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cds").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf"),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.gene_spans").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mini.sortu"),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mm2.splice.bed"),
+                    path(process.out.reference[0][1]).resolve("ref_annot.pep").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.prot_info.dbm").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.fai"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.mm2"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ndb"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nhr"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nin").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.njs").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.not"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nsq"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ntf"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nto"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/build.ok"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrLength.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrNameLength.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrName.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrStart.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonGeTrInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/geneInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Genome"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/genomeParameters.txt").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Log.out").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/transcriptInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
+                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dbm").exists(),
+                    path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
+                    process.out.versions
+                ).match()
         }
 
     }
diff --git a/modules/local/starfusion/build/tests/main.nf.test.snap b/modules/local/starfusion/build/tests/main.nf.test.snap
index 5e5e99acf..6ccede318 100644
--- a/modules/local/starfusion/build/tests/main.nf.test.snap
+++ b/modules/local/starfusion/build/tests/main.nf.test.snap
@@ -1,252 +1,174 @@
 {
     "STARFUSION_BUILD - human - minigenome": {
         "content": [
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-                            "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
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-                            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
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-                            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
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-                        "blast_pairs.idx:md5,884cc8c9886409f7ec0fdf8586ffb78d",
-                        "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                        "fusion_annot_lib.idx:md5,39ab4cd5eab589c5fce9b4bfed82c729",
-                        "pfam_domains.dbm:md5,04c75694f8e36461b43d1c693fdf3414",
-                        "ref_annot.cdna.fa:md5,fe801b66cfab298c575971401e93c18c",
-                        "ref_annot.cdna.fa.idx:md5,3c19e33d5424174f1a3de1f21f6746ff",
-                        "ref_annot.cds:md5,4376fb48bd8bf47f13854b3f6bba8297",
-                        "ref_annot.cdsplus.fa:md5,71e98a5b5d8cf371ec4b5db32c19120e",
-                        "ref_annot.cdsplus.fa.idx:md5,8cb47913bfb0d73d3f55cd5ceafa8bfe",
-                        "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                        "ref_annot.gtf.gene_spans:md5,f178dd54a1f81174dfd203e212937e63",
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-                        "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                        "ref_annot.pep:md5,fe3f58b430d99c06c816521ac439c4df",
-                        "ref_annot.prot_info.dbm:md5,6983b8dccd3e3f920566fab403a15090",
-                        "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
-                        "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
-                        "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
-                        "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
-                        "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
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-                        "ref_genome.fa.njs:md5,7350b9e036410da25ed2c0d717649221",
-                        "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
-                        "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
-                        "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
-                        "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
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-                            "Log.out:md5,944cceca8093617ab4ece780628459f5",
-                            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
-                            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
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-                            "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
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-                            "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
-                            "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
-                            "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
-                            "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                            "genomeParameters.txt:md5,aabd07882af60af4bba0438a475e4e1a",
-                            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
-                            "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
-                            "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
-                            "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2"
-                        ],
-                        "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                        "trans.blast.align_coords.align_coords.dbm:md5,6000e63ebabe57652cfa8858ef6cc484",
-                        "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
-                    ]
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-                        "PFAM.domtblout.dat.gz:md5,6d8ba244e68072be23895ec22c8a8ce4",
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-                            "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
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-                            "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
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-                        "ref_annot.cdna.fa:md5,fe801b66cfab298c575971401e93c18c",
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-                        "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
-                    ]
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-            }
+            "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+            true,
+            true,
+            "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
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+            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
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+            true,
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+            "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
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+            true,
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+            "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+            "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
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+            true,
+            "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+            "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+            "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+            "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+            "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+            "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+            "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+            "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+            true,
+            true,
+            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+            "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+            "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+            "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+            "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+            true,
+            "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
+            null
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-10T22:11:39.460127957"
+        "timestamp": "2024-12-11T15:32:32.307712922"
     },
     "STARFUSION_BUILD - human - minigenome - stub": {
         "content": [
             {
                 "0": [
                     [
-                        "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        {
+                            "id": "minigenome fasta"
+                        },
+                        [
+                            "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
                     ]
                 ],
                 "reference": [
                     [
-                        "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        {
+                            "id": "minigenome fasta"
+                        },
+                        [
+                            "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
                     ]
                 ]
             }
@@ -255,6 +177,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-10T22:12:14.50896742"
+        "timestamp": "2024-12-11T15:05:16.428455255"
     }
-}
\ No newline at end of file
+}

From 1b649b46013bc48d09ddc630799b99c02d4f1eb2 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 11 Dec 2024 16:59:05 +0000
Subject: [PATCH 204/887] test: add fusion_annot_lib so that stub does not fail

---
 tests/test_stub.nf.test | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 41c7194f0..b0601a9f7 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -14,6 +14,7 @@ nextflow_pipeline {
             params {
                 outdir          = "$outputDir"
                 fastp_trim      = true
+                fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
             }
         }
 
@@ -44,6 +45,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir          = "$outputDir"
+                fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
             }
         }
 

From 85563b931302f4f58e7db4c99b26d22e92bc4d33 Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Wed, 11 Dec 2024 18:25:06 +0100
Subject: [PATCH 205/887] init test with local reference

---
 modules/local/fusioncatcher/detect/main.nf    |  2 +-
 modules/local/fusioncatcher/download/main.nf  |  2 +-
 .../local/fusioncatcher_workflow/main.nf      |  2 +-
 .../fusioncatcher_workflow/tests/main.nf.test |  6 ++---
 .../tests/main.nf.test.snap                   | 25 -------------------
 5 files changed, 6 insertions(+), 31 deletions(-)
 delete mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index ddbacce1e..4b615fc38 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -21,7 +21,7 @@ process FUSIONCATCHER {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def reads = fasta.toString().replace(" ", ",")
+    def reads = fastqs.toString().replace(" ", ",")
     def single_end = meta.single_end ? "--single-end" : ""
     """
     fusioncatcher.py \\
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index b0659a153..8a82d9da7 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -18,7 +18,7 @@ process FUSIONCATCHER_DOWNLOAD {
     def args2 = task.ext.args2 ?: ''
     def human_version = "v102"
     def url = "http://sourceforge.net/projects/fusioncatcher/files/data/human_${human_version}.tar.gz.aa"
-    def meta = [ id: "human_${human_version}" ]
+    meta = [ id: "human_${human_version}" ]
     """
     if wget --spider "$url" 2>/dev/null; then
         wget $args $url
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index c268545a7..3bf7c9e9a 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -10,7 +10,7 @@ workflow FUSIONCATCHER_WORKFLOW {
         fusioncatcher_fusions   // path, string
 
     main:
-        ch_versions = Channel.empty()
+        ch_versions   = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_fusioncatcher.txt", checkIfExists: true)
 
         if (( run_fusioncatcher || all) && !fusioninspector_only ) {
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index 4d91798ca..e4614e8de 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -11,7 +11,7 @@ nextflow_workflow {
     // Test
     test("FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4") {
 
-        setup {
+        /*setup {
             // Download reference files for fusioncatch
             run("FUSIONCATCHER_DOWNLOAD") {
                 script "../../../../modules/local/fusioncatcher/download/main.nf"
@@ -21,7 +21,7 @@ nextflow_workflow {
                 """
                 }
             }
-        }
+        }*/
 
         when {
             workflow {
@@ -37,7 +37,7 @@ nextflow_workflow {
                     ] )
 
                 // ch_references
-                input[1] = FUSIONCATCHER_DOWNLOAD.out.reference
+                input[1] = Channel.fromPath("/scratch/moebbs/NEXTFLOW_CODE/reference_rnafusion/human_v102").map{ [ [id: "human_v102"], it] } //FUSIONCATCHER_DOWNLOAD.out.reference
 
                 // fusioncatcher (boolean)
                 input[2] = true
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 19d409a0a..000000000
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,25 +0,0 @@
-{
-    "FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    
-                ],
-                "fusions": [
-                    
-                ],
-                "versions": [
-                    
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-09T20:39:28.49363464"
-    }
-}
\ No newline at end of file

From 4fd46f4e97034c94957e6140112d07965461426b Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Alan=20M=C3=B6bbs?= <alanmmobbs@gmail.com>
Date: Wed, 11 Dec 2024 18:19:10 +0000
Subject: [PATCH 206/887] PR changes

---
 subworkflows/local/arriba_workflow/main.nf    |  11 +-
 .../local/arriba_workflow/tests/main.nf.test  | 147 +++++++++++++++++-
 .../arriba_workflow/tests/main.nf.test.snap   | 135 +++++-----------
 workflows/rnafusion.nf                        |   5 +
 4 files changed, 193 insertions(+), 105 deletions(-)

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 28a0a0420..5f59916a2 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -27,14 +27,14 @@ workflow ARRIBA_WORKFLOW {
         ch_cram_index = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
-        if (( arriba || all) && !fusioninspector_only) {
+        if (( arriba || all ) && !fusioninspector_only) {
 
             STAR_FOR_ARRIBA(
                 reads,
                 ch_starindex_ref,
                 ch_gtf,
                 star_ignore_sjdbgtf,
-                '',                       // seq_platform, should be a params like other pipelines
+                '',
                 seq_center
             )
 
@@ -42,7 +42,7 @@ workflow ARRIBA_WORKFLOW {
 
             if ( arriba_fusions ) {
 
-                ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) ) // Should this be done in the main script?
+                ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
                     .map { meta, reads, fusions -> [ meta, fusions ] }
                 ch_arriba_fusion_fail = ch_dummy_file
 
@@ -52,7 +52,7 @@ workflow ARRIBA_WORKFLOW {
                     STAR_FOR_ARRIBA.out.bam,
                     ch_fasta,
                     ch_gtf,
-                    ch_arriba_ref_blacklist.map{ it[1] },       // should we update nf-core module to includes meta?
+                    ch_arriba_ref_blacklist.map{ it[1] },
                     ch_arriba_ref_known_fusions.map{ it[1] },
                     ch_arriba_ref_cytobands.map{ it[1] },
                     ch_arriba_ref_protein_domains.map{ it[1] }
@@ -80,8 +80,7 @@ workflow ARRIBA_WORKFLOW {
             }
 
         } else {
-            // Not sure how this dummy file can be useful
-            // If this tool can be skipped, why not just emitting an empty channel?
+
             ch_arriba_fusions = reads
                 .combine(Channel.value( file(ch_dummy_file, checkIfExists: true ) ) )
                 .map { meta, reads, fusions -> [ meta, fusions ] }
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
index 96786a5b0..e49a3768c 100644
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test
@@ -125,13 +125,146 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { assert snapshot(workflow.out).match() }
+                { with(workflow.out) {
+                    assert snapshot(
+                            file(fusions[0][1]),
+                            file(fusions_fail[0]),
+                            file(cram_index[0][1]).name,
+                            file(cram_index[0][2]).name,
+                            versions.collect{ file(it) }
+                        ).match()
+                    }
+                }
             )
         }
     }
 
 
-    // TEST #2 WITHOUT INDEXING
+    // Test #2 With arriba_fusions file
+    test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - External fusion file") {
+
+        setup {
+            // Create genome index for STAR
+            run("STAR_GENOMEGENERATE") {
+                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
+                process {
+                """
+                    // FASTA
+                    input[0] = Channel.fromPath(
+                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+
+                    // GTF
+                    input[1] = Channel.fromPath(
+                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+                    """
+                }
+            }
+        }
+
+        when {
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [ id: "test_fastqs" ],
+                        [
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
+                        ]
+                    ] )
+
+                // ch_gtf
+                input[1] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_fasta
+                input[2] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_starindex_ref
+                input[3] = STAR_GENOMEGENERATE.out.index
+
+                // ch_arriba_ref_blacklist
+                input[4] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_known_fusions
+                input[5] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_cytobands
+                input[6] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_arriba_ref_protein_domains
+                input[7] =
+                    Channel.fromPath(
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // arriba (boolean)
+                input[8] = true
+
+                // all (boolean)
+                input[9] = true
+
+                // fusioninspector_only  (boolean)
+                input[10] = false
+
+                // star_ignore_sjdbgtf (boolean)
+                input[11] = false
+
+                // seq_center (string)
+                input[12] = 'test_center'
+
+                // arriba_fusions (string path)
+                input[13] = "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv"
+
+                // cram (array)
+                input[14] = [ 'arriba' ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { with(workflow.out) {
+                    assert snapshot(
+                            fusions[0].size() == 2,
+                            fusions_fail.size() == 1,
+                            file(cram_index[0][1]).name,
+                            file(cram_index[0][2]).name,
+                            versions.collect{ file(it) }
+                        ).match()
+                    }
+                }
+            )
+        }
+    }
+
+    // TEST #3 WITHOUT INDEXING
     test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - cram = []") {
 
         setup {
@@ -241,7 +374,15 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { assert snapshot(workflow.out).match() }
+                { with(workflow.out) {
+                    assert snapshot(
+                            file(fusions[0][1]),
+                            file(fusions_fail[0]),
+                            cram_index.size() == 0,
+                            versions.collect{ file(it) }
+                        ).match()
+                    }
+                }
             )
         }
     }
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
index 3aa777e80..2057827a2 100644
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
@@ -1,114 +1,57 @@
 {
+    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - External fusion file": {
+        "content": [
+            true,
+            true,
+            "test_fastqs_star_for_arriba_sorted.cram",
+            "test_fastqs_star_for_arriba_sorted.cram.crai",
+            [
+                "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
+                "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
+                "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
+                "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-11T15:53:59.18258718"
+    },
     "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.tsv"
-                    ]
-                ],
-                "1": [
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.discarded.tsv"
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/test_fastqs_star_for_arriba_sorted.cram",
-                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/test_fastqs_star_for_arriba_sorted.cram.crai"
-                    ]
-                ],
-                "3": [
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/5b/18880ae27c2497bddad57c4b7ba766/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/48/58af6204cc601a1a0e6e826a117f31/versions.yml"
-                ],
-                "cram_index": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/test_fastqs_star_for_arriba_sorted.cram",
-                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/test_fastqs_star_for_arriba_sorted.cram.crai"
-                    ]
-                ],
-                "fusions": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.tsv"
-                    ]
-                ],
-                "fusions_fail": [
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/test_fastqs.arriba.fusions.discarded.tsv"
-                ],
-                "versions": [
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/da/0878f3451a0f8db34ffd9b178974e6/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/5b/18880ae27c2497bddad57c4b7ba766/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/b5/57dffeefbc4801b0f7c4af2c8530e6/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/c8/482130c0382c0d0318b9e98d0650da/versions.yml",
-                    "/workspace/rnafusion/.nf-test/tests/7899df1159e4d52047799028a4a24a9a/work/48/58af6204cc601a1a0e6e826a117f31/versions.yml"
-                ]
-            }
+            "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d",
+            "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98",
+            "test_fastqs_star_for_arriba_sorted.cram",
+            "test_fastqs_star_for_arriba_sorted.cram.crai",
+            [
+                "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
+                "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
+                "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
+                "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
+                "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
+            ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-09T18:02:58.142697295"
+        "timestamp": "2024-12-11T15:43:48.053656601"
     },
     "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - cram = []": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
-                    ]
-                ],
-                "1": [
-                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
-                ],
-                "2": [
-                    
-                ],
-                "3": [
-                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
-                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9"
-                ],
-                "cram_index": [
-                    
-                ],
-                "fusions": [
-                    [
-                        {
-                            "id": "test_fastqs"
-                        },
-                        "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d"
-                    ]
-                ],
-                "fusions_fail": [
-                    "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98"
-                ],
-                "versions": [
-                    "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
-                    "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9"
-                ]
-            }
+            "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d",
+            "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98",
+            true,
+            [
+                "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
+                "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9"
+            ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-09T18:15:28.965848873"
+        "timestamp": "2024-12-11T16:07:37.079418154"
     }
 }
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 0908b30c3..0c8a6e5fb 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -85,6 +85,11 @@ workflow RNAFUSION {
     //
     // SUBWORKFLOW:  Run STAR alignment and Arriba
     //
+
+    // TODO: add params.seq_platform and pass it as argument to arriba_workflow
+    // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe inputed from samplesheet?
+    // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
+
     ARRIBA_WORKFLOW (
         ch_reads_all,
         ch_gtf,

From 6a50a0bc8fd212492352536f8492c2f27ef4db0f Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Wed, 11 Dec 2024 20:32:47 +0100
Subject: [PATCH 207/887] create test with full reference locally

---
 modules/local/fusioncatcher/detect/main.nf    |  6 +--
 .../fusioncatcher_workflow/tests/main.nf.test |  5 +--
 .../tests/main.nf.test.snap                   | 37 +++++++++++++++++++
 3 files changed, 42 insertions(+), 6 deletions(-)
 create mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 4b615fc38..c7ba7bfb4 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -1,5 +1,5 @@
 process FUSIONCATCHER {
-    tag "$meta.id - $meta2.id"
+    tag "$meta.id"
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
@@ -7,7 +7,7 @@ process FUSIONCATCHER {
 
     input:
     tuple val(meta), path(fastqs, stageAs: "input/*")
-    tuple val(meta2), path(reference, stageAs: "reference/*")
+    tuple val(meta2), path(reference)
 
     output:
     tuple val(meta), path("*.fusioncatcher.fusion-genes.txt")   , optional:true  , emit: fusions
@@ -25,7 +25,7 @@ process FUSIONCATCHER {
     def single_end = meta.single_end ? "--single-end" : ""
     """
     fusioncatcher.py \\
-        -d reference \\
+        -d ${reference} \\
         -i input \\
         -p $task.cpus \\
         -o . \\
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index e4614e8de..89d2f78ad 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -11,7 +11,7 @@ nextflow_workflow {
     // Test
     test("FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4") {
 
-        /*setup {
+        setup {
             // Download reference files for fusioncatch
             run("FUSIONCATCHER_DOWNLOAD") {
                 script "../../../../modules/local/fusioncatcher/download/main.nf"
@@ -21,8 +21,7 @@ nextflow_workflow {
                 """
                 }
             }
-        }*/
-
+        
         when {
             workflow {
                 """
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..522bc131b
--- /dev/null
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -0,0 +1,37 @@
+{
+    "FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_fastqs",
+                            "single_end": false
+                        },
+                        "test_fastqs.fusioncatcher.fusion-genes.txt:md5,c826a24c49abfcec8164c478e1e74892"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,38af82f57bcacbd86c21109a3d425e62"
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test_fastqs",
+                            "single_end": false
+                        },
+                        "test_fastqs.fusioncatcher.fusion-genes.txt:md5,c826a24c49abfcec8164c478e1e74892"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,38af82f57bcacbd86c21109a3d425e62"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.0"
+        },
+        "timestamp": "2024-12-11T19:59:17.754866"
+    }
+}
\ No newline at end of file

From 9bacad7c76f05c9b956dc2fe1b6351cc14608e40 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 12 Dec 2024 11:24:26 +0000
Subject: [PATCH 208/887] Template update for nf-core/tools version 3.1.0

---
 .github/CONTRIBUTING.md                       |  12 +-
 .github/workflows/awsfulltest.yml             |  21 +-
 .github/workflows/branch.yml                  |  18 +-
 .github/workflows/ci.yml                      |   2 +-
 .github/workflows/download_pipeline.yml       |   8 +-
 .github/workflows/fix-linting.yml             |   4 +-
 .github/workflows/linting.yml                 |  10 +-
 .github/workflows/linting_comment.yml         |   2 +-
 .github/workflows/release-announcements.yml   |   2 +-
 .../workflows/template_version_comment.yml    |   2 +-
 .gitpod.yml                                   |  11 +-
 .nf-core.yml                                  |   8 +-
 .vscode/settings.json                         |   3 +
 conf/base.config                              |   2 +-
 conf/modules.config                           |   1 +
 docs/usage.md                                 |  32 +-
 modules.json                                  |   8 +-
 modules/nf-core/fastqc/main.nf                |   2 +-
 modules/nf-core/fastqc/meta.yml               |   1 +
 nextflow.config                               |  53 ++-
 nextflow_schema.json                          |   6 +
 ro-crate-metadata.json                        | 338 ++++++++++++++++++
 .../utils_nfcore_rnafusion_pipeline/main.nf   |   7 +-
 .../nf-core/utils_nextflow_pipeline/main.nf   |   2 +
 .../tests/main.workflow.nf.test               |  10 +-
 .../nf-core/utils_nfcore_pipeline/main.nf     |  89 ++---
 .../tests/main.function.nf.test               |  46 +--
 .../tests/main.function.nf.test.snap          |  30 --
 .../utils_nfschema_plugin/tests/main.nf.test  |   4 +-
 workflows/rnafusion.nf                        |   2 +-
 30 files changed, 518 insertions(+), 218 deletions(-)
 create mode 100644 .vscode/settings.json
 create mode 100644 ro-crate-metadata.json

diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index 7d143af54..b10a5a24f 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -1,4 +1,4 @@
-# nf-core/rnafusion: Contributing Guidelines
+# `nf-core/rnafusion`: Contributing Guidelines
 
 Hi there!
 Many thanks for taking an interest in improving nf-core/rnafusion.
@@ -55,9 +55,9 @@ These tests are run both with the latest available version of `Nextflow` and als
 
 :warning: Only in the unlikely and regretful event of a release happening with a bug.
 
-- On your own fork, make a new branch `patch` based on `upstream/master`.
+- On your own fork, make a new branch `patch` based on `upstream/main` or `upstream/master`.
 - Fix the bug, and bump version (X.Y.Z+1).
-- A PR should be made on `master` from patch to directly this particular bug.
+- Open a pull-request from `patch` to `main`/`master` with the changes.
 
 ## Getting help
 
@@ -65,13 +65,13 @@ For further information/help, please consult the [nf-core/rnafusion documentatio
 
 ## Pipeline contribution conventions
 
-To make the nf-core/rnafusion code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written.
+To make the `nf-core/rnafusion` code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written.
 
 ### Adding a new step
 
 If you wish to contribute a new step, please use the following coding standards:
 
-1. Define the corresponding input channel into your new process from the expected previous process channel
+1. Define the corresponding input channel into your new process from the expected previous process channel.
 2. Write the process block (see below).
 3. Define the output channel if needed (see below).
 4. Add any new parameters to `nextflow.config` with a default (see below).
@@ -84,7 +84,7 @@ If you wish to contribute a new step, please use the following coding standards:
 
 ### Default values
 
-Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope.
+Parameters should be initialised / defined with default values within the `params` scope in `nextflow.config`.
 
 Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`.
 
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 68975fb0d..2281992a2 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -1,11 +1,12 @@
 name: nf-core AWS full size tests
-# This workflow is triggered on PRs opened against the master branch.
+# This workflow is triggered on PRs opened against the main/master branch.
 # It can be additionally triggered manually with GitHub actions workflow dispatch button.
 # It runs the -profile 'test_full' on AWS batch
 
 on:
   pull_request:
     branches:
+      - main
       - master
   workflow_dispatch:
   pull_request_review:
@@ -18,18 +19,30 @@ jobs:
     if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch'
     runs-on: ubuntu-latest
     steps:
-      - uses: octokit/request-action@v2.x
+      - name: Get PR reviews
+        uses: octokit/request-action@v2.x
+        if: github.event_name != 'workflow_dispatch'
         id: check_approvals
+        continue-on-error: true
         with:
-          route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews
+          route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews?per_page=100
         env:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-      - id: test_variables
+
+      - name: Check for approvals
+        if: ${{ failure() && github.event_name != 'workflow_dispatch' }}
+        run: |
+          echo "No review approvals found. At least 2 approvals are required to run this action automatically."
+          exit 1
+
+      - name: Check for enough approvals (>=2)
+        id: test_variables
         if: github.event_name != 'workflow_dispatch'
         run: |
           JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}'
           CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length')
           test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required
+
       - name: Launch workflow via Seqera Platform
         uses: seqeralabs/action-tower-launch@v2
         # TODO nf-core: You can customise AWS full pipeline tests as required
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 19704d239..00e20510e 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -1,15 +1,17 @@
 name: nf-core branch protection
-# This workflow is triggered on PRs to master branch on the repository
-# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev`
+# This workflow is triggered on PRs to `main`/`master` branch on the repository
+# It fails when someone tries to make a PR against the nf-core `main`/`master` branch instead of `dev`
 on:
   pull_request_target:
-    branches: [master]
+    branches:
+      - main
+      - master
 
 jobs:
   test:
     runs-on: ubuntu-latest
     steps:
-      # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
+      # PRs to the nf-core repo main/master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
       - name: Check PRs
         if: github.repository == 'nf-core/rnafusion'
         run: |
@@ -22,7 +24,7 @@ jobs:
         uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
         with:
           message: |
-            ## This PR is against the `master` branch :x:
+            ## This PR is against the `${{github.event.pull_request.base.ref}}` branch :x:
 
             * Do not close this PR
             * Click _Edit_ and change the `base` to `dev`
@@ -32,9 +34,9 @@ jobs:
 
             Hi @${{ github.event.pull_request.user.login }},
 
-            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch.
-            The `master` branch on nf-core repositories should always contain code from the latest release.
-            Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
+            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) ${{github.event.pull_request.base.ref}} branch.
+            The ${{github.event.pull_request.base.ref}} branch on nf-core repositories should always contain code from the latest release.
+            Because of this, PRs to ${{github.event.pull_request.base.ref}} are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
 
             You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
             Note that even after this, the test will continue to show as failing until you push a new commit.
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 04fba0396..68412b2ce 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -45,7 +45,7 @@ jobs:
             profile: "singularity"
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Set up Nextflow
         uses: nf-core/setup-nextflow@v2
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 713dc3e73..2576cc0c7 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -2,7 +2,7 @@ name: Test successful pipeline download with 'nf-core pipelines download'
 
 # Run the workflow when:
 #  - dispatched manually
-#  - when a PR is opened or reopened to master branch
+#  - when a PR is opened or reopened to main/master branch
 #  - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev.
 on:
   workflow_dispatch:
@@ -17,9 +17,11 @@ on:
       - edited
       - synchronize
     branches:
+      - main
       - master
   pull_request_target:
     branches:
+      - main
       - master
 
 env:
@@ -35,7 +37,7 @@ jobs:
       - name: Disk space cleanup
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
           python-version: "3.12"
           architecture: "x64"
@@ -69,7 +71,7 @@ jobs:
           --outdir ./${{ env.REPOTITLE_LOWERCASE }} \
           --compress "none" \
           --container-system 'singularity' \
-          --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io" \
+          --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io/library/" \
           --container-cache-utilisation 'amend' \
           --download-configuration 'yes'
 
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml
index df750880c..1e4ccb869 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
@@ -32,7 +32,7 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
       # Install and run pre-commit
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
           python-version: "3.12"
 
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index a502573c5..dbd52d5a2 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -14,10 +14,10 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Set up Python 3.12
-        uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+        uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
           python-version: "3.12"
 
@@ -31,12 +31,12 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
           python-version: "3.12"
           architecture: "x64"
@@ -74,7 +74,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4
+        uses: actions/upload-artifact@b4b15b8c7c6ac21ea08fcf65892d2ee8f75cf882 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 42e519bfa..0bed96d36 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@bf251b5aa9c2f7eeb574a96ee720e24f801b7c11 # v6
+        uses: dawidd6/action-download-artifact@80620a5d27ce0ae443b965134db88467fc607b43 # v7
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index c6ba35df4..450b1d5e4 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -31,7 +31,7 @@ jobs:
     runs-on: ubuntu-latest
 
     steps:
-      - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
           python-version: "3.10"
       - name: Install dependencies
diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml
index e8aafe44d..537529bc1 100644
--- a/.github/workflows/template_version_comment.yml
+++ b/.github/workflows/template_version_comment.yml
@@ -9,7 +9,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
         with:
           ref: ${{ github.event.pull_request.head.sha }}
 
diff --git a/.gitpod.yml b/.gitpod.yml
index 461186376..83599f633 100644
--- a/.gitpod.yml
+++ b/.gitpod.yml
@@ -6,12 +6,5 @@ tasks:
       nextflow self-update
 
 vscode:
-  extensions: # based on nf-core.nf-core-extensionpack
-    #- esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code
-    - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
-    - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
-    - mechatroner.rainbow-csv # Highlight columns in csv files in different colors
-    - nextflow.nextflow # Nextflow syntax highlighting
-    - oderwat.indent-rainbow # Highlight indentation level
-    - streetsidesoftware.code-spell-checker # Spelling checker for source code
-    - charliermarsh.ruff # Code linter Ruff
+  extensions:
+    - nf-core.nf-core-extensionpack # https://github.com/nf-core/vscode-extensionpack
diff --git a/.nf-core.yml b/.nf-core.yml
index 097a52b0e..37d9e4cd7 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,10 +1,10 @@
-bump_version: null
 lint:
   files_unchanged:
     - .github/CONTRIBUTING.md
     - .github/PULL_REQUEST_TEMPLATE.md
-nf_core_version: 3.0.2
-org_path: null
+    - conf/igenomes.config
+    - conf/igenomes_ignored.config
+nf_core_version: 3.1.0
 repository_type: pipeline
 template:
   author: Martin Proks, Annick Renevey
@@ -14,6 +14,4 @@ template:
   name: rnafusion
   org: nf-core
   outdir: .
-  skip_features: null
   version: 4.0.0dev
-update: null
diff --git a/.vscode/settings.json b/.vscode/settings.json
new file mode 100644
index 000000000..a33b527cc
--- /dev/null
+++ b/.vscode/settings.json
@@ -0,0 +1,3 @@
+{
+    "markdown.styles": ["public/vscode_markdown.css"]
+}
diff --git a/conf/base.config b/conf/base.config
index f5ec67936..c0779b554 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -20,7 +20,7 @@ process {
     maxErrors     = '-1'
 
     // Process-specific resource requirements
-    // NOTE - Please try and re-use the labels below as much as possible.
+    // NOTE - Please try and reuse the labels below as much as possible.
     //        These labels are used and recognised by default in DSL2 files hosted on nf-core/modules.
     //        If possible, it would be nice to keep the same label naming convention when
     //        adding in your local modules too.
diff --git a/conf/modules.config b/conf/modules.config
index d266a387f..d203d2b6e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -21,6 +21,7 @@ process {
     withName: FASTQC {
         ext.args = '--quiet'
     }
+
     withName: 'MULTIQC' {
         ext.args   = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
         publishDir = [
diff --git a/docs/usage.md b/docs/usage.md
index 01ddc7731..c8a121d09 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -75,9 +75,8 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than
 
 Pipeline settings can be provided in a `yaml` or `json` file via `-params-file <file>`.
 
-:::warning
-Do not use `-c <file>` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args).
-:::
+> [!WARNING]
+> Do not use `-c <file>` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args).
 
 The above pipeline run specified with a params file in yaml format:
 
@@ -106,23 +105,21 @@ nextflow pull nf-core/rnafusion
 
 ### Reproducibility
 
-It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since.
+It is a good idea to specify the pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since.
 
 First, go to the [nf-core/rnafusion releases page](https://github.com/nf-core/rnafusion/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag.
 
 This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports.
 
-To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter.
+To further assist in reproducibility, you can use share and reuse [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter.
 
-:::tip
-If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles.
-:::
+> [!TIP]
+> If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles.
 
 ## Core Nextflow arguments
 
-:::note
-These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen).
-:::
+> [!NOTE]
+> These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen)
 
 ### `-profile`
 
@@ -130,16 +127,15 @@ Use this parameter to choose a configuration profile. Profiles can give configur
 
 Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below.
 
-:::info
-We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
-:::
+> [!IMPORTANT]
+> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
-The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
+The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is suported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
 
 Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important!
 They are loaded in sequence, so later profiles can overwrite earlier profiles.
 
-If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer enviroment.
+If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer environment.
 
 - `test`
   - A profile with a complete configuration for automated testing
@@ -175,13 +171,13 @@ Specify the path to a specific config file (this is a core Nextflow command). Se
 
 ### Resource requests
 
-Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
+Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the pipeline steps, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher resources request (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
 
 To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website.
 
 ### Custom Containers
 
-In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date.
+In some cases, you may wish to change the container or conda environment used by a pipeline steps for a particular tool. By default, nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However, in some cases the pipeline specified version maybe out of date.
 
 To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website.
 
diff --git a/modules.json b/modules.json
index 01a6c107b..d655d7870 100644
--- a/modules.json
+++ b/modules.json
@@ -7,7 +7,7 @@
                 "nf-core": {
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "dc94b6ee04a05ddb9f7ae050712ff30a13149164",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
@@ -21,17 +21,17 @@
                 "nf-core": {
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082",
+                        "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba",
+                        "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c",
+                        "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index d8989f481..752c3a10c 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -24,7 +24,7 @@ process FASTQC {
     // Make list of old name and new name pairs to use for renaming in the bash while loop
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
     def rename_to = old_new_pairs*.join(' ').join(' ')
-    def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ')
+    def renamed_files = old_new_pairs.collect{ _old_name, new_name -> new_name }.join(' ')
 
     // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
     // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index 4827da7af..2b2e62b8a 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -11,6 +11,7 @@ tools:
         FastQC gives general quality metrics about your reads.
         It provides information about the quality score distribution
         across your reads, the per base sequence content (%A/C/G/T).
+
         You get information about adapter contamination and other
         overrepresented sequences.
       homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
diff --git a/nextflow.config b/nextflow.config
index 872c08044..4383d5421 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -38,8 +38,7 @@ params {
     show_hidden                  = false
     version                      = false
     pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/'
-
-    // Config options
+    trace_report_suffix          = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')// Config options
     config_profile_name        = null
     config_profile_description = null
 
@@ -153,6 +152,13 @@ profiles {
         executor.name           = 'local'
         executor.cpus           = 4
         executor.memory         = 8.GB
+        process {
+            resourceLimits = [
+                memory: 8.GB,
+                cpus  : 4,
+                time  : 1.h
+            ]
+        }
     }
     test      { includeConfig 'conf/test.config'      }
     test_full { includeConfig 'conf/test_full.config' }
@@ -201,30 +207,49 @@ set -C # No clobber - prevent output redirection from overwriting files.
 // Disable process selector warnings by default. Use debug profile to enable warnings.
 nextflow.enable.configProcessNamesValidation = false
 
-def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
 timeline {
     enabled = true
-    file    = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html"
+    file    = "${params.outdir}/pipeline_info/execution_timeline_${params.trace_report_suffix}.html"
 }
 report {
     enabled = true
-    file    = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html"
+    file    = "${params.outdir}/pipeline_info/execution_report_${params.trace_report_suffix}.html"
 }
 trace {
     enabled = true
-    file    = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt"
+    file    = "${params.outdir}/pipeline_info/execution_trace_${params.trace_report_suffix}.txt"
 }
 dag {
     enabled = true
-    file    = "${params.outdir}/pipeline_info/pipeline_dag_${trace_timestamp}.html"
+    file    = "${params.outdir}/pipeline_info/pipeline_dag_${params.trace_report_suffix}.html"
 }
 
 manifest {
     name            = 'nf-core/rnafusion'
-    author          = """Martin Proks, Annick Renevey"""
+    author          = """Martin Proks, Annick Renevey""" // The author field is deprecated from Nextflow version 24.10.0, use contributors instead
+    contributors    = [
+        // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0
+        [
+            name: 'Martin Proks',
+            affiliation: '',
+            email: '',
+            github: '',
+            contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor')
+            orcid: ''
+        ],
+        [
+            name: ' Annick Renevey',
+            affiliation: '',
+            email: '',
+            github: '',
+            contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor')
+            orcid: ''
+        ],
+    ]
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = """Nextflow rnafusion analysis pipeline, part of the nf-core community."""
     mainScript      = 'main.nf'
+    defaultBranch   = 'master'
     nextflowVersion = '!>=24.04.2'
     version         = '4.0.0dev'
     doi             = ''
@@ -237,9 +262,10 @@ plugins {
 
 validation {
     defaultIgnoreParams = ["genomes"]
+    monochromeLogs = params.monochrome_logs
     help {
         enabled = true
-        command = "nextflow run $manifest.name -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
+        command = "nextflow run nf-core/rnafusion -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
         fullParameter = "help_full"
         showHiddenParameter = "show_hidden"
         beforeText = """
@@ -249,15 +275,15 @@ validation {
 \033[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \033[0;33m}  {\033[0m
 \033[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \033[0;32m\\`-._,-`-,\033[0m
                                         \033[0;32m`._,._,\'\033[0m
-\033[0;35m  ${manifest.name} ${manifest.version}\033[0m
+\033[0;35m  nf-core/rnafusion ${manifest.version}\033[0m
 -\033[2m----------------------------------------------------\033[0m-
 """
-        afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { "  https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""}
+        afterText = """${manifest.doi ? "\n* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { "    https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""}
 * The nf-core framework
     https://doi.org/10.1038/s41587-020-0439-x
 
 * Software dependencies
-    https://github.com/${manifest.name}/blob/master/CITATIONS.md
+    https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md
 """
     }
     summary {
@@ -265,6 +291,3 @@ validation {
         afterText = validation.help.afterText
     }
 }
-
-// Load modules.config for DSL2 module specific options
-includeConfig 'conf/modules.config'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 021e105a2..69a4e5dbd 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -218,6 +218,12 @@
                     "description": "Base URL or local path to location of pipeline test dataset files",
                     "default": "https://raw.githubusercontent.com/nf-core/test-datasets/",
                     "hidden": true
+                },
+                "trace_report_suffix": {
+                    "type": "string",
+                    "fa_icon": "far calendar",
+                    "description": "Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.",
+                    "hidden": true
                 }
             }
         }
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
new file mode 100644
index 000000000..f1d7090ab
--- /dev/null
+++ b/ro-crate-metadata.json
@@ -0,0 +1,338 @@
+{
+    "@context": [
+        "https://w3id.org/ro/crate/1.1/context",
+        {
+            "GithubService": "https://w3id.org/ro/terms/test#GithubService",
+            "JenkinsService": "https://w3id.org/ro/terms/test#JenkinsService",
+            "PlanemoEngine": "https://w3id.org/ro/terms/test#PlanemoEngine",
+            "TestDefinition": "https://w3id.org/ro/terms/test#TestDefinition",
+            "TestInstance": "https://w3id.org/ro/terms/test#TestInstance",
+            "TestService": "https://w3id.org/ro/terms/test#TestService",
+            "TestSuite": "https://w3id.org/ro/terms/test#TestSuite",
+            "TravisService": "https://w3id.org/ro/terms/test#TravisService",
+            "definition": "https://w3id.org/ro/terms/test#definition",
+            "engineVersion": "https://w3id.org/ro/terms/test#engineVersion",
+            "instance": "https://w3id.org/ro/terms/test#instance",
+            "resource": "https://w3id.org/ro/terms/test#resource",
+            "runsOn": "https://w3id.org/ro/terms/test#runsOn"
+        }
+    ],
+    "@graph": [
+        {
+            "@id": "./",
+            "@type": "Dataset",
+            "creativeWorkStatus": "InProgress",
+            "datePublished": "2024-12-12T11:24:21+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "hasPart": [
+                {
+                    "@id": "main.nf"
+                },
+                {
+                    "@id": "assets/"
+                },
+                {
+                    "@id": "conf/"
+                },
+                {
+                    "@id": "docs/"
+                },
+                {
+                    "@id": "docs/images/"
+                },
+                {
+                    "@id": "modules/"
+                },
+                {
+                    "@id": "modules/nf-core/"
+                },
+                {
+                    "@id": "workflows/"
+                },
+                {
+                    "@id": "subworkflows/"
+                },
+                {
+                    "@id": "nextflow.config"
+                },
+                {
+                    "@id": "README.md"
+                },
+                {
+                    "@id": "nextflow_schema.json"
+                },
+                {
+                    "@id": "CHANGELOG.md"
+                },
+                {
+                    "@id": "LICENSE"
+                },
+                {
+                    "@id": "CODE_OF_CONDUCT.md"
+                },
+                {
+                    "@id": "CITATIONS.md"
+                },
+                {
+                    "@id": "modules.json"
+                },
+                {
+                    "@id": "docs/usage.md"
+                },
+                {
+                    "@id": "docs/output.md"
+                },
+                {
+                    "@id": ".nf-core.yml"
+                },
+                {
+                    "@id": ".pre-commit-config.yaml"
+                },
+                {
+                    "@id": ".prettierignore"
+                }
+            ],
+            "isBasedOn": "https://github.com/nf-core/rnafusion",
+            "license": "MIT",
+            "mainEntity": {
+                "@id": "main.nf"
+            },
+            "mentions": [
+                {
+                    "@id": "#e018c319-519d-4a99-bdba-7c9c8860b896"
+                }
+            ],
+            "name": "nf-core/rnafusion"
+        },
+        {
+            "@id": "ro-crate-metadata.json",
+            "@type": "CreativeWork",
+            "about": {
+                "@id": "./"
+            },
+            "conformsTo": [
+                {
+                    "@id": "https://w3id.org/ro/crate/1.1"
+                },
+                {
+                    "@id": "https://w3id.org/workflowhub/workflow-ro-crate/1.0"
+                }
+            ]
+        },
+        {
+            "@id": "main.nf",
+            "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
+            "creator": [
+                {
+                    "@id": "https://orcid.org/0000-0001-9017-591X"
+                },
+                {
+                    "@id": "#phil.ewels@scilifelab.se"
+                },
+                {
+                    "@id": "https://orcid.org/0000-0002-8178-3128"
+                },
+                {
+                    "@id": "#max.u.garcia@gmail.com"
+                }
+            ],
+            "dateCreated": "",
+            "dateModified": "2024-12-12T11:24:21Z",
+            "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
+            "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
+            "license": ["MIT"],
+            "maintainer": [
+                {
+                    "@id": "https://orcid.org/0000-0002-8178-3128"
+                }
+            ],
+            "name": ["nf-core/rnafusion"],
+            "programmingLanguage": {
+                "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow"
+            },
+            "sdPublisher": {
+                "@id": "https://nf-co.re/"
+            },
+            "url": ["https://github.com/nf-core/rnafusion", "https://nf-co.re/rnafusion/dev/"],
+            "version": ["4.0.0dev"]
+        },
+        {
+            "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow",
+            "@type": "ComputerLanguage",
+            "identifier": {
+                "@id": "https://www.nextflow.io/"
+            },
+            "name": "Nextflow",
+            "url": {
+                "@id": "https://www.nextflow.io/"
+            },
+            "version": "!>=24.04.2"
+        },
+        {
+            "@id": "#e018c319-519d-4a99-bdba-7c9c8860b896",
+            "@type": "TestSuite",
+            "instance": [
+                {
+                    "@id": "#36a96043-5ef5-4bc3-86eb-32712383dda8"
+                }
+            ],
+            "mainEntity": {
+                "@id": "main.nf"
+            },
+            "name": "Test suite for nf-core/rnafusion"
+        },
+        {
+            "@id": "#36a96043-5ef5-4bc3-86eb-32712383dda8",
+            "@type": "TestInstance",
+            "name": "GitHub Actions workflow for testing nf-core/rnafusion",
+            "resource": "repos/nf-core/rnafusion/actions/workflows/ci.yml",
+            "runsOn": {
+                "@id": "https://w3id.org/ro/terms/test#GithubService"
+            },
+            "url": "https://api.github.com"
+        },
+        {
+            "@id": "https://w3id.org/ro/terms/test#GithubService",
+            "@type": "TestService",
+            "name": "Github Actions",
+            "url": {
+                "@id": "https://github.com"
+            }
+        },
+        {
+            "@id": "assets/",
+            "@type": "Dataset",
+            "description": "Additional files"
+        },
+        {
+            "@id": "conf/",
+            "@type": "Dataset",
+            "description": "Configuration files"
+        },
+        {
+            "@id": "docs/",
+            "@type": "Dataset",
+            "description": "Markdown files for documenting the pipeline"
+        },
+        {
+            "@id": "docs/images/",
+            "@type": "Dataset",
+            "description": "Images for the documentation files"
+        },
+        {
+            "@id": "modules/",
+            "@type": "Dataset",
+            "description": "Modules used by the pipeline"
+        },
+        {
+            "@id": "modules/nf-core/",
+            "@type": "Dataset",
+            "description": "nf-core modules"
+        },
+        {
+            "@id": "workflows/",
+            "@type": "Dataset",
+            "description": "Main pipeline workflows to be executed in main.nf"
+        },
+        {
+            "@id": "subworkflows/",
+            "@type": "Dataset",
+            "description": "Smaller subworkflows"
+        },
+        {
+            "@id": "nextflow.config",
+            "@type": "File",
+            "description": "Main Nextflow configuration file"
+        },
+        {
+            "@id": "README.md",
+            "@type": "File",
+            "description": "Basic pipeline usage information"
+        },
+        {
+            "@id": "nextflow_schema.json",
+            "@type": "File",
+            "description": "JSON schema for pipeline parameter specification"
+        },
+        {
+            "@id": "CHANGELOG.md",
+            "@type": "File",
+            "description": "Information on changes made to the pipeline"
+        },
+        {
+            "@id": "LICENSE",
+            "@type": "File",
+            "description": "The license - should be MIT"
+        },
+        {
+            "@id": "CODE_OF_CONDUCT.md",
+            "@type": "File",
+            "description": "The nf-core code of conduct"
+        },
+        {
+            "@id": "CITATIONS.md",
+            "@type": "File",
+            "description": "Citations needed when using the pipeline"
+        },
+        {
+            "@id": "modules.json",
+            "@type": "File",
+            "description": "Version information for modules from nf-core/modules"
+        },
+        {
+            "@id": "docs/usage.md",
+            "@type": "File",
+            "description": "Usage documentation"
+        },
+        {
+            "@id": "docs/output.md",
+            "@type": "File",
+            "description": "Output documentation"
+        },
+        {
+            "@id": ".nf-core.yml",
+            "@type": "File",
+            "description": "nf-core configuration file, configuring template features and linting rules"
+        },
+        {
+            "@id": ".pre-commit-config.yaml",
+            "@type": "File",
+            "description": "Configuration file for pre-commit hooks"
+        },
+        {
+            "@id": ".prettierignore",
+            "@type": "File",
+            "description": "Ignore file for prettier"
+        },
+        {
+            "@id": "https://nf-co.re/",
+            "@type": "Organization",
+            "name": "nf-core",
+            "url": "https://nf-co.re/"
+        },
+        {
+            "@id": "https://orcid.org/0000-0001-9017-591X",
+            "@type": "Person",
+            "email": "rickard.hammaren@scilifelab.se",
+            "name": "Rickard Hammar\u00e9n"
+        },
+        {
+            "@id": "#phil.ewels@scilifelab.se",
+            "@type": "Person",
+            "email": "phil.ewels@scilifelab.se",
+            "name": "Phil Ewels"
+        },
+        {
+            "@id": "https://orcid.org/0000-0002-8178-3128",
+            "@type": "Person",
+            "email": "mproksik@gmail.com",
+            "name": "Martin Proks"
+        },
+        {
+            "@id": "#max.u.garcia@gmail.com",
+            "@type": "Person",
+            "email": "max.u.garcia@gmail.com",
+            "name": "Maxime Garcia"
+        }
+    ]
+}
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 834a4e5c6..df68ef3ab 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -116,7 +116,8 @@ workflow PIPELINE_COMPLETION {
 
     main:
     summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
-
+    def multiqc_reports = multiqc_report.toList()
+    
     //
     // Completion email and summary
     //
@@ -129,7 +130,7 @@ workflow PIPELINE_COMPLETION {
                 plaintext_email,
                 outdir,
                 monochrome_logs,
-                multiqc_report.toList()
+                multiqc_reports.getVal(),
             )
         }
 
@@ -225,7 +226,7 @@ def toolBibliographyText() {
 }
 
 def methodsDescriptionText(mqc_methods_yaml) {
-    // Convert  to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file
+    // Convert  to a named map so can be used as with familiar NXF ${workflow} variable syntax in the MultiQC YML file
     def meta = [:]
     meta.workflow = workflow.toMap()
     meta["manifest_map"] = workflow.manifest.toMap()
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
index 0fcbf7b3f..d6e593e85 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf
@@ -92,10 +92,12 @@ def checkCondaChannels() {
         channels = config.channels
     }
     catch (NullPointerException e) {
+        log.debug(e)
         log.warn("Could not verify conda channel configuration.")
         return null
     }
     catch (IOException e) {
+        log.debug(e)
         log.warn("Could not verify conda channel configuration.")
         return null
     }
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
index ca964ce8e..02dbf094c 100644
--- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test
@@ -52,10 +52,12 @@ nextflow_workflow {
         }
 
         then {
-            assertAll(
-                { assert workflow.success },
-                { assert workflow.stdout.contains("nextflow_workflow v9.9.9") }
-            )
+            expect {
+                with(workflow) {
+                    assert success
+                    assert "nextflow_workflow v9.9.9" in stdout
+                }
+            }
         }
     }
 
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index 5cb7bafef..bfd258760 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -56,21 +56,6 @@ def checkProfileProvided(nextflow_cli_args) {
     }
 }
 
-//
-// Citation string for pipeline
-//
-def workflowCitation() {
-    def temp_doi_ref = ""
-    def manifest_doi = workflow.manifest.doi.tokenize(",")
-    // Handling multiple DOIs
-    // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers
-    // Removing ` ` since the manifest.doi is a string and not a proper list
-    manifest_doi.each { doi_ref ->
-        temp_doi_ref += "  https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n"
-    }
-    return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + "* The nf-core framework\n" + "  https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + "  https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md"
-}
-
 //
 // Generate workflow version string
 //
@@ -150,33 +135,6 @@ def paramsSummaryMultiqc(summary_params) {
     return yaml_file_text
 }
 
-//
-// nf-core logo
-//
-def nfCoreLogo(monochrome_logs=true) {
-    def colors = logColours(monochrome_logs) as Map
-    String.format(
-        """\n
-        ${dashedLine(monochrome_logs)}
-                                                ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset}
-        ${colors.blue}        ___     __   __   __   ___     ${colors.green}/,-._.--~\'${colors.reset}
-        ${colors.blue}  |\\ | |__  __ /  ` /  \\ |__) |__         ${colors.yellow}}  {${colors.reset}
-        ${colors.blue}  | \\| |       \\__, \\__/ |  \\ |___     ${colors.green}\\`-._,-`-,${colors.reset}
-                                                ${colors.green}`._,._,\'${colors.reset}
-        ${colors.purple}  ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset}
-        ${dashedLine(monochrome_logs)}
-        """.stripIndent()
-    )
-}
-
-//
-// Return dashed line
-//
-def dashedLine(monochrome_logs=true) {
-    def colors = logColours(monochrome_logs) as Map
-    return "-${colors.dim}----------------------------------------------------${colors.reset}-"
-}
-
 //
 // ANSII colours used for terminal logging
 //
@@ -245,28 +203,24 @@ def logColours(monochrome_logs=true) {
     return colorcodes
 }
 
-//
-// Attach the multiqc report to email
-//
-def attachMultiqcReport(multiqc_report) {
-    def mqc_report = null
-    try {
-        if (workflow.success) {
-            mqc_report = multiqc_report.getVal()
-            if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) {
-                if (mqc_report.size() > 1) {
-                    log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one")
-                }
-                mqc_report = mqc_report[0]
-            }
+// Return a single report from an object that may be a Path or List
+//
+def getSingleReport(multiqc_reports) {
+    if (multiqc_reports instanceof Path) {
+        return multiqc_reports
+    } else if (multiqc_reports instanceof List) {
+        if (multiqc_reports.size() == 0) {
+            log.warn("[${workflow.manifest.name}] No reports found from process 'MULTIQC'")
+            return null
+        } else if (multiqc_reports.size() == 1) {
+            return multiqc_reports.first()
+        } else {
+            log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one")
+            return multiqc_reports.first()
         }
+    } else {
+        return null
     }
-    catch (Exception all) {
-        if (multiqc_report) {
-            log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email")
-        }
-    }
-    return mqc_report
 }
 
 //
@@ -320,7 +274,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     email_fields['summary']      = summary << misc_fields
 
     // On success try attach the multiqc report
-    def mqc_report = attachMultiqcReport(multiqc_report)
+    def mqc_report = getSingleReport(multiqc_report)
 
     // Check if we are only sending emails on failure
     def email_address = email
@@ -340,7 +294,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     def email_html    = html_template.toString()
 
     // Render the sendmail template
-    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit
+    def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as MemoryUnit
     def smail_fields           = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()]
     def sf                     = new File("${workflow.projectDir}/assets/sendmail_template.txt")
     def sendmail_template      = engine.createTemplate(sf).make(smail_fields)
@@ -351,14 +305,17 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi
     if (email_address) {
         try {
             if (plaintext_email) {
-new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML')            }
+                new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML')
+            }
             // Try to send HTML e-mail using sendmail
             def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html")
             sendmail_tf.withWriter { w -> w << sendmail_html }
             ['sendmail', '-t'].execute() << sendmail_html
             log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-")
         }
-        catch (Exception all) {
+        catch (Exception msg) {
+            log.debug(msg.toString())
+            log.debug("Trying with mail instead of sendmail")
             // Catch failures and try with plaintext
             def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address]
             mail_cmd.execute() << email_html
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
index 1dc317f8f..f117040cb 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test
@@ -41,26 +41,14 @@ nextflow_function {
         }
     }
 
-    test("Test Function workflowCitation") {
-
-        function "workflowCitation"
-
-        then {
-            assertAll(
-                { assert function.success },
-                { assert snapshot(function.result).match() }
-            )
-        }
-    }
-
-    test("Test Function nfCoreLogo") {
+    test("Test Function without logColours") {
 
-        function "nfCoreLogo"
+        function "logColours"
 
         when {
             function {
                 """
-                input[0] = false
+                input[0] = true
                 """
             }
         }
@@ -73,9 +61,8 @@ nextflow_function {
         }
     }
 
-    test("Test Function dashedLine") {
-
-        function "dashedLine"
+    test("Test Function with logColours") {
+        function "logColours"
 
         when {
             function {
@@ -93,14 +80,13 @@ nextflow_function {
         }
     }
 
-    test("Test Function without logColours") {
-
-        function "logColours"
+    test("Test Function getSingleReport with a single file") {
+        function "getSingleReport"
 
         when {
             function {
                 """
-                input[0] = true
+                input[0] = file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true)
                 """
             }
         }
@@ -108,18 +94,22 @@ nextflow_function {
         then {
             assertAll(
                 { assert function.success },
-                { assert snapshot(function.result).match() }
+                { assert function.result.contains("test.tsv") }
             )
         }
     }
 
-    test("Test Function with logColours") {
-        function "logColours"
+    test("Test Function getSingleReport with multiple files") {
+        function "getSingleReport"
 
         when {
             function {
                 """
-                input[0] = false
+                input[0] = [
+                    file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + '/generic/tsv/network.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + '/generic/tsv/expression.tsv', checkIfExists: true)
+                ]
                 """
             }
         }
@@ -127,7 +117,9 @@ nextflow_function {
         then {
             assertAll(
                 { assert function.success },
-                { assert snapshot(function.result).match() }
+                { assert function.result.contains("test.tsv") },
+                { assert !function.result.contains("network.tsv") },
+                { assert !function.result.contains("expression.tsv") }
             )
         }
     }
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
index 1037232c9..02c670141 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap
@@ -17,26 +17,6 @@
         },
         "timestamp": "2024-02-28T12:02:59.729647"
     },
-    "Test Function nfCoreLogo": {
-        "content": [
-            "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n                                        \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m        ___     __   __   __   ___     \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \u001b[0;33m}  {\u001b[0m\n\u001b[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \u001b[0;32m\\`-._,-`-,\u001b[0m\n                                        \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m  nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-28T12:03:10.562934"
-    },
-    "Test Function workflowCitation": {
-        "content": [
-            "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n  https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n  https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n  https://github.com/nextflow_workflow/blob/master/CITATIONS.md"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-28T12:03:07.019761"
-    },
     "Test Function without logColours": {
         "content": [
             {
@@ -95,16 +75,6 @@
         },
         "timestamp": "2024-02-28T12:03:17.969323"
     },
-    "Test Function dashedLine": {
-        "content": [
-            "-\u001b[2m----------------------------------------------------\u001b[0m-"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-28T12:03:14.366181"
-    },
     "Test Function with logColours": {
         "content": [
             {
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
index 842dc432a..8fb301648 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
@@ -42,7 +42,7 @@ nextflow_workflow {
 
             params {
                 test_data   = ''
-                outdir      = 1
+                outdir      = null
             }
 
             workflow {
@@ -94,7 +94,7 @@ nextflow_workflow {
 
             params {
                 test_data   = ''
-                outdir      = 1
+                outdir      = null
             }
 
             workflow {
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 53c51b00d..e80985a2f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -39,7 +39,7 @@ workflow RNAFUSION {
     softwareVersionsToYAML(ch_versions)
         .collectFile(
             storeDir: "${params.outdir}/pipeline_info",
-            name: 'nf_core_'  + 'pipeline_software_' +  'mqc_'  + 'versions.yml',
+            name: 'nf_core_'  +  'rnafusion_software_'  + 'mqc_'  + 'versions.yml',
             sort: true,
             newLine: true
         ).set { ch_collated_versions }

From ec373b317aadcfc7db831c632bc308569469400c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 12 Dec 2024 14:18:50 +0000
Subject: [PATCH 209/887] fix: add dependencies versions and update container

---
 modules/local/starfusion/build/environment.yml | 2 +-
 modules/local/starfusion/build/main.nf         | 5 +----
 2 files changed, 2 insertions(+), 5 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index d02ab2d69..eacee484a 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -6,6 +6,6 @@ dependencies:
   - bioconda::hmmer=3.4
   - bioconda::minimap2=2.28
   - bioconda::samtools=1.6
-  - bioconda::star=2.7.11a
   - bioconda::star-fusion=1.14.0
+  - bioconda::star=2.7.11a
   - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index f3014ca93..6dc654d0e 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,7 +3,7 @@ process STARFUSION_BUILD {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:63e3d21ca68ea531'
+    container 'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'
 
     input:
     tuple val(meta), path(fasta)
@@ -15,9 +15,6 @@ process STARFUSION_BUILD {
     tuple val(meta), path("ctat_genome_lib_build_dir"), emit: reference
 
     script:
-    if (dfam_species != "human" && dfam_species != "mouse") {
-        error "Invalid species for --dfam_db. Only 'human' or 'mouse' are accepted. Provided: ${dfam_species}"
-    }
     def args = task.ext.args ?: ''
     """
     prep_genome_lib.pl \\

From 99033b5d342a92362b6414bf7e03ee2260fd277b Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 12 Dec 2024 14:19:25 +0000
Subject: [PATCH 210/887] refactor: add readlength as ext.args

---
 conf/modules.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/modules.config b/conf/modules.config
index abe0e79d4..0eb1441d9 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -329,6 +329,7 @@ process {
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
+        ext.args = "--max_readlength ${params.read_length}"
     }
 
     withName: 'STARFUSION_DOWNLOAD' {

From 17dd8b4b4ede749cc9a66bad5846859b232bbf19 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 12 Dec 2024 15:03:59 +0000
Subject: [PATCH 211/887] test: update stub structure

---
 modules/local/starfusion/build/main.nf        |  97 +++++++++++-----
 .../starfusion/build/tests/main.nf.test.snap  | 106 ++++++++++++++++--
 2 files changed, 167 insertions(+), 36 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 6dc654d0e..484389b12 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -34,41 +34,88 @@ process STARFUSION_BUILD {
 
     stub:
     """
-    mkdir ctat_genome_lib_build_dir
+    mkdir -p ctat_genome_lib_build_dir
+
     touch ctat_genome_lib_build_dir/AnnotFilterRule.pm
-    gzip -c /dev/null > ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
-    touch ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
-    touch ctat_genome_lib_build_dir/ref_genome.fa.mm2
-    touch ctat_genome_lib_build_dir/ref_genome.fa.ntf
     gzip -c /dev/null > ctat_genome_lib_build_dir/blast_pairs.dat.gz
-    touch ctat_genome_lib_build_dir/ref_annot.cdna.fa
-    touch ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-    touch ctat_genome_lib_build_dir/ref_genome.fa.ndb
-    touch ctat_genome_lib_build_dir/ref_genome.fa.nto
     touch ctat_genome_lib_build_dir/blast_pairs.idx
+
+    mkdir -p ctat_genome_lib_build_dir/__chkpts
+    touch ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok
+    touch ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok
+    touch ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok
+    touch ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok
+    touch ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok
+    touch ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok
+    touch ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok
+    touch ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok
+    touch ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok
+    touch ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok
+    touch ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok
+    touch ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok
+    touch ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok
+    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok
+    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok
+    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok
+    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok
+    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok
+    touch ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok
+    touch ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok
+    touch ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok
+    touch ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok
+    touch ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok
+
+    gzip -c /dev/null > ctat_genome_lib_build_dir/fusion_annot_lib.gz
+    touch ctat_genome_lib_build_dir/fusion_annot_lib.idx
+    touch ctat_genome_lib_build_dir/pfam_domains.dbm
+    gzip -c /dev/null > ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
+
+    touch ctat_genome_lib_build_dir/ref_annot.cdna.fa
     touch ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx
-    touch ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed
-    touch ctat_genome_lib_build_dir/ref_genome.fa.nhr
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx
-    touch ctat_genome_lib_build_dir/__chkpts
     touch ctat_genome_lib_build_dir/ref_annot.cds
-    touch ctat_genome_lib_build_dir/ref_annot.pep
-    touch ctat_genome_lib_build_dir/ref_genome.fa.nin
-    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat
-    gzip -c /dev/null > ctat_genome_lib_build_dir/fusion_annot_lib.gz
     touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
-    touch ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
-    touch ctat_genome_lib_build_dir/ref_genome.fa.njs
-    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
-    touch ctat_genome_lib_build_dir/fusion_annot_lib.idx
     touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
-    touch ctat_genome_lib_build_dir/ref_genome.fa
-    touch ctat_genome_lib_build_dir/ref_genome.fa.not
-    gzip -c /dev/null > ctat_genome_lib_build_dir/trans.blast.dat.gz
-    touch ctat_genome_lib_build_dir/pfam_domains.dbm
     touch ctat_genome_lib_build_dir/ref_annot.gtf
+    touch ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
+    touch ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
+    touch ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed
+    touch ctat_genome_lib_build_dir/ref_annot.pep
+    touch ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
+
+    touch ctat_genome_lib_build_dir/ref_genome.fa
     touch ctat_genome_lib_build_dir/ref_genome.fa.fai
+    touch ctat_genome_lib_build_dir/ref_genome.fa.mm2
+    touch ctat_genome_lib_build_dir/ref_genome.fa.ndb
+    touch ctat_genome_lib_build_dir/ref_genome.fa.nhr
+    touch ctat_genome_lib_build_dir/ref_genome.fa.nin
+    touch ctat_genome_lib_build_dir/ref_genome.fa.njs
+    touch ctat_genome_lib_build_dir/ref_genome.fa.not
     touch ctat_genome_lib_build_dir/ref_genome.fa.nsq
+    touch ctat_genome_lib_build_dir/ref_genome.fa.ntf
+    touch ctat_genome_lib_build_dir/ref_genome.fa.nto
+
+    mkdir -p ctat_genome_lib_build_dir/ref_genome.fa.star.idx
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab
+    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab
+
+    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat
+    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
+    gzip -c /dev/null > ctat_genome_lib_build_dir/trans.blast.dat.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/local/starfusion/build/tests/main.nf.test.snap b/modules/local/starfusion/build/tests/main.nf.test.snap
index 6ccede318..e50db541e 100644
--- a/modules/local/starfusion/build/tests/main.nf.test.snap
+++ b/modules/local/starfusion/build/tests/main.nf.test.snap
@@ -62,12 +62,12 @@
             "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
             "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
             "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-            "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+            "Genome:md5,21aac51c5062f73d6305ae77691f5d4e",
             true,
             true,
-            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
-            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
-            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+            "SA:md5,4d6ee7c82328aa304300e51ab5677ff9",
+            "SAindex:md5,2d3498f7d84a69bbc65fe9de1887820c",
+            "sjdbInfo.txt:md5,0ad0c108e6d02ec541d6adeffbdea6fd",
             "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
             "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
             "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
@@ -80,7 +80,7 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-11T15:32:32.307712922"
+        "timestamp": "2024-12-12T14:57:13.528134845"
     },
     "STARFUSION_BUILD - human - minigenome - stub": {
         "content": [
@@ -93,7 +93,31 @@
                         [
                             "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
                             "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -121,7 +145,25 @@
                             "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
                             "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
@@ -136,7 +178,31 @@
                         [
                             "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "__chkpts:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
                             "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -164,7 +230,25 @@
                             "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.star.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
                             "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
@@ -177,6 +261,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-11T15:05:16.428455255"
+        "timestamp": "2024-12-12T14:57:33.861849482"
     }
-}
+}
\ No newline at end of file

From 4f078b5d068b7d954af7066fbaad4026b0e4c291 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 16 Dec 2024 15:43:00 +0000
Subject: [PATCH 212/887] test: add nf-test to `starfusion`

---
 modules/local/starfusion/detect/main.nf       |   3 +-
 .../starfusion/detect/tests/main.nf.test      |  87 ++++++++++++++
 .../starfusion/detect/tests/main.nf.test.snap | 112 ++++++++++++++++++
 3 files changed, 201 insertions(+), 1 deletion(-)
 create mode 100644 modules/local/starfusion/detect/tests/main.nf.test
 create mode 100644 modules/local/starfusion/detect/tests/main.nf.test.snap

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 273967707..10bc6582c 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -18,12 +18,13 @@ process STARFUSION {
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
     def fasta = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
+    def junction_arg = junction ? "-J ${junction}": ""
     def args = task.ext.args ?: ''
     """
     STAR-Fusion \\
         --genome_lib_dir $reference \\
         $fasta \\
-        -J $junction \\
+        $junction_arg \\
         --CPU $task.cpus \\
         --examine_coding_effect \\
         --output_dir . \\
diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
new file mode 100644
index 000000000..75e0bfdd7
--- /dev/null
+++ b/modules/local/starfusion/detect/tests/main.nf.test
@@ -0,0 +1,87 @@
+nextflow_process {
+
+    name "Test Process STARFUSION"
+    script "../main.nf"
+    process "STARFUSION"
+
+    setup {
+        run("STARFUSION_BUILD") {
+            script "../../../starfusion/build/main.nf"
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                ]
+                input[1] = [
+                    [ id:'minigenome gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                ]
+
+                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input [3] = "human"
+                """
+            }
+        }
+    }
+
+    test("Should run without failures") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                    ], // reads
+                    [] // empty list for junction, as we don't have a pre-computed Chimeric.out.junction file
+                ]
+                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
+                """
+            }
+        }
+
+        then {
+            assert process.success
+            assert snapshot(
+                process.out.fusions,
+                process.out.abridged,
+                process.out.coding_effect,
+                process.out.versions
+            ).match()
+        }
+
+    }
+
+    test("Should create stub files") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test_stub', single_end:false ],
+                    [
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                    ],
+                    [] // empty list for junction
+                ]
+                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
+                """
+            }
+        }
+
+        then {
+            assert process.success
+            assert snapshot(
+                process.out
+            ).match()
+        }
+
+    }
+
+}
diff --git a/modules/local/starfusion/detect/tests/main.nf.test.snap b/modules/local/starfusion/detect/tests/main.nf.test.snap
new file mode 100644
index 000000000..2532b33d6
--- /dev/null
+++ b/modules/local/starfusion/detect/tests/main.nf.test.snap
@@ -0,0 +1,112 @@
+{
+    "Should create stub files": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,4131f39f483637d95446231588add8f1"
+                ],
+                "abridged": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coding_effect": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,4131f39f483637d95446231588add8f1"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-16T14:47:21.862981618"
+    },
+    "Should run without failures": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.starfusion.fusion_predictions.tsv:md5,2adb2aeb44ea98736fb7572e7b5de5c6"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.starfusion.abridged.tsv:md5,f37f1cd07a3a6ff41e131dbe369bc093"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.starfusion.abridged.coding_effect.tsv:md5,8f5739b0351a725ee96cdf52860d680e"
+                ]
+            ],
+            [
+                "versions.yml:md5,4131f39f483637d95446231588add8f1"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-16T14:47:06.459753683"
+    }
+}
\ No newline at end of file

From 9f3a0d68ecc645c9d6171c52f52bf229d71f2518 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 16 Dec 2024 15:43:16 +0000
Subject: [PATCH 213/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 690024b2c..f4d5e6fd9 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -20,6 +20,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `QC_WORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
 - Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/577)
+- Add nf-test to local module: `STARFUSION`. [#585](https://github.com/nf-core/rnafusion/pull/585)
 
 ### Changed
 

From dbb45c91eeb1d52d9e63d93a7b0d5e515bf5c8ec Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 17 Dec 2024 13:42:01 +0100
Subject: [PATCH 214/887] fix test config resources

---
 conf/test.config        | 9 +++++++++
 conf/test_build.config  | 9 +++++++++
 conf/test_cosmic.config | 9 +++++++++
 3 files changed, 27 insertions(+)

diff --git a/conf/test.config b/conf/test.config
index 3cafa252b..d042d9239 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -17,3 +17,12 @@ params {
     // Input data
     input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
 }
+
+// Limit and standardize resources for github actions and reproducibility
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
\ No newline at end of file
diff --git a/conf/test_build.config b/conf/test_build.config
index 616d734f0..1e3245711 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -22,3 +22,12 @@ params {
 
     skip_salmon_index          = true
 }
+
+// Limit and standardize resources for github actions and reproducibility
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
\ No newline at end of file
diff --git a/conf/test_cosmic.config b/conf/test_cosmic.config
index cf199da4d..7101deb50 100644
--- a/conf/test_cosmic.config
+++ b/conf/test_cosmic.config
@@ -20,3 +20,12 @@ params {
     cosmic_username        = secrets.COSMIC_USERNAME
     cosmic_passwd          = secrets.COSMIC_PASSWD
 }
+
+// Limit and standardize resources for github actions and reproducibility
+process {
+    resourceLimits = [
+        cpus: 4,
+        memory: '15.GB',
+        time: '1.h'
+    ]
+}
\ No newline at end of file

From 4245c543cab4ed66ce60efc737a04455a995e75d Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 17 Dec 2024 13:42:59 +0100
Subject: [PATCH 215/887] add ctatsplicing module

---
 conf/modules.config                           |  12 ++
 .../ctatsplicing/startocancerintrons/main.nf  |  71 +++++++
 .../startocancerintrons/tests/main.nf.test    |  69 +++++++
 .../tests/main.nf.test.snap                   | 191 ++++++++++++++++++
 4 files changed, 343 insertions(+)
 create mode 100644 modules/local/ctatsplicing/startocancerintrons/main.nf
 create mode 100644 modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test
 create mode 100644 modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test.snap

diff --git a/conf/modules.config b/conf/modules.config
index 264d1c87e..9a72b994e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -49,6 +49,18 @@ process {
         ]
     }
 
+    withName: 'CTATSPLICING_STARTOCANCERINTRONS' {
+        ext.args = {[
+            bam ? "--vis" : "",
+            "--sample_name ${meta.id}",
+        ].join(" ")}
+        publishDir = [
+            path: { "${params.outdir}/ctat_splicing" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
     withName: 'ENSEMBL_DOWNLOAD' {
         publishDir = [
             path: { "${params.genomes_base}/ensembl" },
diff --git a/modules/local/ctatsplicing/startocancerintrons/main.nf b/modules/local/ctatsplicing/startocancerintrons/main.nf
new file mode 100644
index 000000000..ba7a61102
--- /dev/null
+++ b/modules/local/ctatsplicing/startocancerintrons/main.nf
@@ -0,0 +1,71 @@
+process CTATSPLICING_STARTOCANCERINTRONS {
+    tag "$meta.id"
+    label 'process_single'
+
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://data.broadinstitute.org/Trinity/CTAT_SINGULARITY/CTAT-SPLICING/ctat_splicing.v0.0.2.simg' :
+        'docker.io/trinityctat/ctat_splicing:0.0.2' }"
+
+    input:
+    tuple val(meta), path(sj), path(junction), path(bam), path(bai)
+    tuple val(meta2), path(genome_lib)
+
+    output:
+    tuple val(meta), path("*.cancer_intron_reads.sorted.bam")       , emit: cancer_introns_sorted_bam
+    tuple val(meta), path("*.cancer_intron_reads.sorted.bam.bai")   , emit: cancer_introns_sorted_bai
+    tuple val(meta), path("*.gene_reads.sorted.sifted.bam")         , emit: gene_reads_sorted_bam
+    tuple val(meta), path("*.gene_reads.sorted.sifted.bam.bai")     , emit: gene_reads_sorted_bai
+    tuple val(meta), path("*.cancer.introns")                       , emit: cancer_introns
+    tuple val(meta), path("*.cancer.introns.prelim")                , emit: cancer_introns_prelim
+    tuple val(meta), path("*${prefix}.introns")                     , emit: introns
+    tuple val(meta), path("*.introns.for_IGV.bed")                  , emit: introns_igv_bed, optional: true
+    tuple val(meta), path("*.ctat-splicing.igv.html")               , emit: igv_html, optional: true
+    tuple val(meta), path("*.igv.tracks")                           , emit: igv_tracks, optional: true
+    tuple val(meta), path("*.chckpts")                              , emit: chckpts
+    path "versions.yml"                                             , emit: versions
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def bam_arg = bam ? "--bam_file ${bam}" : ""
+    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def create_index = bam && !bai ? "samtools index ${bam}" : ""
+    """
+    ${create_index}
+    /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \\
+        --SJ_tab_file ${sj} \\
+        --chimJ_file ${junction} \\
+        ${bam_arg} \\
+        --output_prefix ${prefix} \\
+        --ctat_genome_lib ${genome_lib} \\
+        ${args}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ctat-splicing: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def create_igv_files = args.contains("--vis") ? "touch ${prefix}.introns.for_IGV.bed && touch ${prefix}.ctat-splicing.igv.html && touch ${prefix}.igv.tracks" : ""
+    """
+    ${create_igv_files}
+    touch ${prefix}.cancer_intron_reads.sorted.bam
+    touch ${prefix}.cancer_intron_reads.sorted.bam.bai
+    touch ${prefix}.gene_reads.sorted.sifted.bam
+    touch ${prefix}.gene_reads.sorted.sifted.bam.bai
+    touch ${prefix}.cancer.introns
+    touch ${prefix}.cancer.introns.prelim
+    touch ${prefix}.introns
+    touch ${prefix}.chckpts
+
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ctat-splicing: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test b/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test
new file mode 100644
index 000000000..dad961c47
--- /dev/null
+++ b/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test
@@ -0,0 +1,69 @@
+nextflow_process {
+
+    name "Test Process CTATSPLICING_STARTOCANCERINTRONS"
+    script "../main.nf"
+    process "CTATSPLICING_STARTOCANCERINTRONS"
+    options "-stub"
+
+    test("test without BAM") {
+
+        when {
+            params {
+                outdir = "tests/results"
+            }
+            process {
+                """
+                input[0] = [
+                    [id:"test"],
+                    file("test.SJ.out.tab"),
+                    file("test.Chimeric.out.junctions"),
+                    [],
+                    []
+                ]
+                input[1] = [
+                    [id:"reference"],
+                    file("ctat_genome_lib")
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
+            )
+        }
+    }
+
+    test("test with BAM") {
+
+        when {
+            params {
+                outdir = "tests/results"
+            }
+            process {
+                """
+                input[0] = [
+                    [id:"test"],
+                    file("test.SJ.out.tab"),
+                    file("test.Chimeric.out.junctions"),
+                    file("test.Aligned.sortedByCoord.out.bam"),
+                    []
+                ]
+                input[1] = [
+                    [id:"reference"],
+                    file("ctat_genome_lib")
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
+            )
+        }
+    }
+}
diff --git a/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test.snap b/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test.snap
new file mode 100644
index 000000000..b0ee34166
--- /dev/null
+++ b/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test.snap
@@ -0,0 +1,191 @@
+{
+    "test without BAM": {
+        "content": [
+            {
+                "cancer_introns": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cancer_introns_prelim": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer.introns.prelim:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cancer_introns_sorted_bai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer_intron_reads.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cancer_introns_sorted_bam": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer_intron_reads.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "chckpts": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.chckpts:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gene_reads_sorted_bai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gene_reads.sorted.sifted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gene_reads_sorted_bam": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gene_reads.sorted.sifted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "igv_html": [
+                    
+                ],
+                "igv_tracks": [
+                    
+                ],
+                "introns": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "introns_igv_bed": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,fcf861a15f9951342a874b6bc476a37e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.1",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-17T13:35:13.723215847"
+    },
+    "test with BAM": {
+        "content": [
+            {
+                "cancer_introns": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cancer_introns_prelim": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer.introns.prelim:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cancer_introns_sorted_bai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer_intron_reads.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cancer_introns_sorted_bam": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.cancer_intron_reads.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "chckpts": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.chckpts:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gene_reads_sorted_bai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gene_reads.sorted.sifted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gene_reads_sorted_bam": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.gene_reads.sorted.sifted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "igv_html": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.ctat-splicing.igv.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "igv_tracks": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.igv.tracks:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "introns": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "introns_igv_bed": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.introns.for_IGV.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,fcf861a15f9951342a874b6bc476a37e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.1",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-12-17T13:33:27.36677449"
+    }
+}
\ No newline at end of file

From fa128b6805a6884a7ff29fbe30159e8fa229a5a3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Dec 2024 14:16:28 +0100
Subject: [PATCH 216/887] avoid using meta for remapping

---
 subworkflows/local/arriba_workflow.nf     | 2 +-
 subworkflows/local/starfusion_workflow.nf | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/arriba_workflow.nf b/subworkflows/local/arriba_workflow.nf
index 5575f5c0d..10ddc16ab 100644
--- a/subworkflows/local/arriba_workflow.nf
+++ b/subworkflows/local/arriba_workflow.nf
@@ -33,7 +33,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
                 ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
-                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ meta, file -> return file}
+                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ it -> it[1]}
             }
 
             if (params.cram.contains('arriba') ){
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index d6a0668a1..f70396f58 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -21,7 +21,7 @@ workflow STARFUSION_WORKFLOW {
         if ((params.starfusion || params.all || params.stringtie) && !params.fusioninspector_only) {
             if (params.starfusion_fusions){
                 ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
-                                        .map { meta, reads, fusions -> [ meta, fusions ] }
+                                        .map { it -> [ it[0], it[2] ] }
             } else {
                 STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
@@ -51,7 +51,7 @@ workflow STARFUSION_WORKFLOW {
         }
         else {
             ch_starfusion_fusions = reads.combine(Channel.value(file(ch_dummy_file, checkIfExists:true)))
-                                    .map { meta, reads, fusions -> [ meta, fusions ] }
+                                    .map { it -> [ it[0], it[2] ] }
             ch_star_stats = Channel.empty()
             ch_star_gene_count = Channel.empty()
         }

From f0259453d818c16dd2a3b40660745d5bffb034c5 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 17 Dec 2024 14:18:55 +0100
Subject: [PATCH 217/887] implement ctatsplicing

---
 conf/modules.config                           |  2 +-
 .../ctatsplicing/startocancerintrons/main.nf  |  4 +-
 nextflow.config                               |  1 +
 nextflow_schema.json                          |  5 ++
 .../local/ctatsplicing_workflow/main.nf       | 31 ++++++++++
 subworkflows/local/starfusion_workflow.nf     | 25 +++++---
 workflows/rnafusion.nf                        | 57 +++++++++++--------
 7 files changed, 91 insertions(+), 34 deletions(-)
 create mode 100644 subworkflows/local/ctatsplicing_workflow/main.nf

diff --git a/conf/modules.config b/conf/modules.config
index 9a72b994e..ed6bc1eba 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -55,7 +55,7 @@ process {
             "--sample_name ${meta.id}",
         ].join(" ")}
         publishDir = [
-            path: { "${params.outdir}/ctat_splicing" },
+            path: { "${params.outdir}/ctatsplicing" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
diff --git a/modules/local/ctatsplicing/startocancerintrons/main.nf b/modules/local/ctatsplicing/startocancerintrons/main.nf
index ba7a61102..30fd5c3f2 100644
--- a/modules/local/ctatsplicing/startocancerintrons/main.nf
+++ b/modules/local/ctatsplicing/startocancerintrons/main.nf
@@ -7,7 +7,7 @@ process CTATSPLICING_STARTOCANCERINTRONS {
         'docker.io/trinityctat/ctat_splicing:0.0.2' }"
 
     input:
-    tuple val(meta), path(sj), path(junction), path(bam), path(bai)
+    tuple val(meta), path(split_junction), path(junction), path(bam), path(bai)
     tuple val(meta2), path(genome_lib)
 
     output:
@@ -33,7 +33,7 @@ process CTATSPLICING_STARTOCANCERINTRONS {
     """
     ${create_index}
     /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \\
-        --SJ_tab_file ${sj} \\
+        --SJ_tab_file ${split_junction} \\
         --chimJ_file ${junction} \\
         ${bam_arg} \\
         --output_prefix ${prefix} \\
diff --git a/nextflow.config b/nextflow.config
index d436182c4..46c2b5175 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -61,6 +61,7 @@ params {
     // Enable or disable tools
     all                        = false
     arriba                     = false
+    ctatsplicing              = false
     fusioncatcher              = false
     starindex                  = false
     starfusion                 = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 017a19fb8..c8618561b 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -143,6 +143,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba output"
                 },
+                "ctatsplicing": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Build or run arriba CTAT-SPLICING"
+                },
                 "ensembl_ref": {
                     "type": "string",
                     "fa_icon": "far fa-file-code",
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
new file mode 100644
index 000000000..a1516397e
--- /dev/null
+++ b/subworkflows/local/ctatsplicing_workflow/main.nf
@@ -0,0 +1,31 @@
+include { CTATSPLICING_STARTOCANCERINTRONS } from '../../../modules/local/ctatsplicing/startocancerintrons'
+
+workflow CTATSPLICING_WORKFLOW {
+    take:
+    split_junctions // [ val(meta), path(split_junctions.SJ.out.tab) ]
+    junctions       // [ val(meta), path(junctions.Chimeric.out.junction) ]
+    aligned_bams    // [ val(meta), path(aligned_bams.Aligned.sortedByCoord.out.bam) ]
+    ctat_genome_lib // [ val(meta2), path(path/to/ctat_genome_lib) ]
+
+    main:
+    def ch_versions = Channel.empty()
+
+    if (params.ctatsplicing || params.all) {
+        def ch_ctatsplicing_input = split_junctions
+            .join(junctions, failOnMismatch:true, failOnDuplicate:true)
+            .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
+            .map { meta, split_junction, junction, bam ->
+                [ meta, split_junction, junction, bam, [] ]
+            }
+        
+        CTATSPLICING_STARTOCANCERINTRONS(
+            ch_ctatsplicing_input,
+            ctat_genome_lib
+        )
+
+    }
+
+    emit:
+    versions              = ch_versions
+}
+
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index de99d8e51..8a9340da0 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -10,15 +10,20 @@ workflow STARFUSION_WORKFLOW {
         ch_chrgtf
         ch_starindex_ref
         ch_fasta
+        ch_starfusion_ref
 
     main:
-        ch_versions = Channel.empty()
-        ch_align = Channel.empty()
-        bam_sorted_indexed = Channel.empty()
+        def ch_versions = Channel.empty()
+        def ch_align = Channel.empty()
+        def ch_starfusion_fusions = Channel.empty()
+        def bam_sorted_indexed = Channel.empty()
+        def ch_bam_align_sorted = Channel.empty()
+        def ch_split_junctions = Channel.empty()
+        def ch_junctions = Channel.empty()
 
         ch_dummy_file = file("$baseDir/assets/dummy_file_starfusion.txt", checkIfExists: true)
 
-        if ((params.starfusion || params.all || params.stringtie) && !params.fusioninspector_only) {
+        if ((params.starfusion || params.all || params.stringtie || params.ctatsplicing) && !params.fusioninspector_only) {
             if (params.starfusion_fusions){
                 ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
                                         .map { meta, reads, fusions -> [ meta, fusions ] }
@@ -32,6 +37,10 @@ workflow STARFUSION_WORKFLOW {
                 bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai)
                 reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
 
+                ch_bam_align_sorted = STAR_FOR_STARFUSION.out.bam_sorted_aligned
+                ch_split_junctions = STAR_FOR_STARFUSION.out.spl_junc_tab
+                ch_junctions = STAR_FOR_STARFUSION.out.junction
+
                 if (params.cram.contains('starfusion')){
                     SAMTOOLS_VIEW_FOR_STARFUSION (bam_sorted_indexed, ch_fasta, [] )
                     ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_STARFUSION.out.versions)
@@ -39,8 +48,8 @@ workflow STARFUSION_WORKFLOW {
                     SAMTOOLS_INDEX_FOR_STARFUSION_CRAM (SAMTOOLS_VIEW_FOR_STARFUSION.out.cram)
                     ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION_CRAM.out.versions)
                 }
-                if (params.starfusion || params.all){
-                    STARFUSION( reads_junction, params.starfusion_ref)
+                if (params.starfusion || params.all || params.ctatsplicing){
+                    STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
                     ch_versions = ch_versions.mix(STARFUSION.out.versions)
                     ch_starfusion_fusions = STARFUSION.out.fusions
                 }
@@ -61,7 +70,9 @@ workflow STARFUSION_WORKFLOW {
         star_gene_count       = ch_star_gene_count
         ch_bam_sorted         = ch_align.ifEmpty([[],[]])
         ch_bam_sorted_indexed = bam_sorted_indexed.ifEmpty([[],[],[]])
+        bam_align_sorted      = ch_bam_align_sorted
+        split_junctions       = ch_split_junctions
+        junctions             = ch_junctions
         versions              = ch_versions
-
     }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 0c8a6e5fb..2f1bbfab2 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -6,6 +6,7 @@
 include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
+include { CTATSPLICING_WORKFLOW         }   from '../subworkflows/local/ctatsplicing_workflow'
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
@@ -22,27 +23,6 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi
 include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 
 
-ch_chrgtf                     = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ref              = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
-ch_starindex_ensembl_ref      = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_refflat                    = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
-ch_rrna_interval              = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
-ch_adapter_fastp              = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta, checkIfExists: true) : Channel.empty()
-ch_fusionreport_ref           = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
-ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_ref     = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
-ch_hgnc_date    = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
-ch_fasta        = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
-ch_gtf          = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
-ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
-ch_transcript   = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
-ch_fai          = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
-
-
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -56,8 +36,29 @@ workflow RNAFUSION {
 
     main:
 
-    ch_versions = Channel.empty()
-    ch_multiqc_files = Channel.empty()
+    def ch_versions = Channel.empty()
+    def ch_multiqc_files = Channel.empty()
+
+    def ch_chrgtf                     = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect()
+    def ch_starindex_ref              = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect()
+    def ch_starindex_ensembl_ref      = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect()
+    def ch_refflat                    = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect()
+    def ch_rrna_interval              = params.starfusion_build ?  Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect()
+    def ch_adapter_fastp              = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta, checkIfExists: true) : Channel.empty()
+    def ch_fusionreport_ref           = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect()
+    def ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect()
+    def ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect()
+    def ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect()
+    def ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect()
+    def ch_hgnc_ref                   = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect()
+    def ch_hgnc_date                  = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect()
+    def ch_fasta                      = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect()
+    def ch_gtf                        = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect()
+    def ch_salmon_index               = Channel.fromPath(params.salmon_index).map { it -> [[id:it.Name], it] }.collect()
+    def ch_transcript                 = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect()
+    def ch_fai                        = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect()
+    def ch_starfusion_ref             = Channel.fromPath(params.starfusion_ref).map { it -> [[id:it.name], it]}.collect()
+
     //
     // MODULE: Run FastQC
     //
@@ -115,7 +116,8 @@ workflow RNAFUSION {
         ch_reads_all,
         ch_chrgtf,
         ch_starindex_ref,
-        ch_fasta
+        ch_fasta,
+        ch_starfusion_ref
     )
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
 
@@ -145,6 +147,13 @@ workflow RNAFUSION {
     )
     ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
 
+    //Run CTAT-splicing
+    CTATSPLICING_WORKFLOW(
+        STARFUSION_WORKFLOW.out.split_junctions,
+        STARFUSION_WORKFLOW.out.junctions,
+        STARFUSION_WORKFLOW.out.bam_align_sorted,
+        ch_starfusion_ref
+    )
 
     //Run fusionInpector
     FUSIONINSPECTOR_WORKFLOW (

From 3268a455bb633220f33462dbae8c42db0aba9657 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 17 Dec 2024 14:33:47 +0100
Subject: [PATCH 218/887] fix pre-commit

---
 conf/test.config                                 | 2 +-
 conf/test_build.config                           | 2 +-
 conf/test_cosmic.config                          | 2 +-
 subworkflows/local/ctatsplicing_workflow/main.nf | 4 ++--
 4 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index d042d9239..b3d325854 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -25,4 +25,4 @@ process {
         memory: '15.GB',
         time: '1.h'
     ]
-}
\ No newline at end of file
+}
diff --git a/conf/test_build.config b/conf/test_build.config
index 1e3245711..70c3a4cec 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -30,4 +30,4 @@ process {
         memory: '15.GB',
         time: '1.h'
     ]
-}
\ No newline at end of file
+}
diff --git a/conf/test_cosmic.config b/conf/test_cosmic.config
index 7101deb50..9cc1bcda9 100644
--- a/conf/test_cosmic.config
+++ b/conf/test_cosmic.config
@@ -28,4 +28,4 @@ process {
         memory: '15.GB',
         time: '1.h'
     ]
-}
\ No newline at end of file
+}
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
index a1516397e..8b279033d 100644
--- a/subworkflows/local/ctatsplicing_workflow/main.nf
+++ b/subworkflows/local/ctatsplicing_workflow/main.nf
@@ -17,15 +17,15 @@ workflow CTATSPLICING_WORKFLOW {
             .map { meta, split_junction, junction, bam ->
                 [ meta, split_junction, junction, bam, [] ]
             }
-        
+
         CTATSPLICING_STARTOCANCERINTRONS(
             ch_ctatsplicing_input,
             ctat_genome_lib
         )
+        ch_versions = ch_versions.mix(CTATSPLICING_STARTOCANCERINTRONS.out.versions.first())
 
     }
 
     emit:
     versions              = ch_versions
 }
-

From a84033b11302d29381ad6ec4ccf52e52883c0ad7 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Tue, 17 Dec 2024 14:42:25 +0100
Subject: [PATCH 219/887] Update nextflow.config

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 46c2b5175..06a1278f4 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -61,7 +61,7 @@ params {
     // Enable or disable tools
     all                        = false
     arriba                     = false
-    ctatsplicing              = false
+    ctatsplicing               = false
     fusioncatcher              = false
     starindex                  = false
     starfusion                 = false

From 4247b12844c276dd3200f42e04c61f2583c86e2c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 17 Dec 2024 14:48:30 +0100
Subject: [PATCH 220/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a5c881c3f..2dfa7a79e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,6 +21,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
 - Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/577)
 - Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
+- Added a new module `CTATSPLICING_STARTOCANCERINTRONS` [#587](https://github.com/nf-core/rnafusion/pull/587)
 
 ### Changed
 

From 5e58fbfb71ee6d39d7b9cfd8841116d40ac4af2b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Dec 2024 15:16:29 +0100
Subject: [PATCH 221/887] use docker containers for fusioninspector,
 fusioncatcher and starfusion

---
 modules/local/fusioncatcher/detect/main.nf        |  3 +--
 modules/local/fusioncatcher/download/main.nf      |  3 +--
 modules/local/fusioninspector/environment.yml     |  7 +------
 modules/local/fusioninspector/main.nf             |  2 +-
 modules/local/starfusion/build/environment.yml    |  7 +------
 modules/local/starfusion/build/main.nf            |  2 +-
 modules/local/starfusion/detect/environment.yml   |  7 +------
 modules/local/starfusion/detect/main.nf           |  2 +-
 modules/local/starfusion/download/environment.yml |  7 +------
 modules/local/starfusion/download/main.nf         |  2 +-
 workflows/build_references.nf                     | 10 +++++-----
 11 files changed, 15 insertions(+), 37 deletions(-)

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 9a0928463..d2c6b929c 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -2,8 +2,7 @@ process FUSIONCATCHER {
     tag "$meta.id"
     label 'process_high'
 
-    conda "${moduleDir}/environment.yml"
-    container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
+    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 65a736077..f11491b9a 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -2,8 +2,7 @@ process FUSIONCATCHER_DOWNLOAD {
     tag "fusioncatcher_download"
     label 'process_medium'
 
-    conda "${moduleDir}/environment.yml"
-    container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
+    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
 
     output:
     path "*"                , emit: reference
diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index 06a9828af..77187e8bf 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -2,9 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::dfam=3.7
-  - bioconda::hmmer=3.4
-  - bioconda::star-fusion=1.7.0
-  - bioconda::trinity=2.15.2
-  - bioconda::samtools=1.21
-  - bioconda::star=2.7.11b
+  - bioconda::star-fusion=1.14.0
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index ae05ceb42..b91d37c9c 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -3,7 +3,7 @@ process FUSIONINSPECTOR {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
+    container 'trinityctat/fusioninspector:2.10.0'
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 06a9828af..77187e8bf 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -2,9 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::dfam=3.7
-  - bioconda::hmmer=3.4
-  - bioconda::star-fusion=1.7.0
-  - bioconda::trinity=2.15.2
-  - bioconda::samtools=1.21
-  - bioconda::star=2.7.11b
+  - bioconda::star-fusion=1.14.0
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 6ac3c6942..9212cdafa 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -2,7 +2,7 @@ process STARFUSION_BUILD {
     tag 'star-fusion'
 
     conda "${moduleDir}/environment.yml"
-    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
+    container 'docker.io/trinityctat/starfusion:1.14.0'
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index 06a9828af..77187e8bf 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -2,9 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::dfam=3.7
-  - bioconda::hmmer=3.4
-  - bioconda::star-fusion=1.7.0
-  - bioconda::trinity=2.15.2
-  - bioconda::samtools=1.21
-  - bioconda::star=2.7.11b
+  - bioconda::star-fusion=1.14.0
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 273967707..94342e99c 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -3,7 +3,7 @@ process STARFUSION {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
+    container 'docker.io/trinityctat/starfusion:1.14.0'
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/environment.yml b/modules/local/starfusion/download/environment.yml
index 06a9828af..77187e8bf 100644
--- a/modules/local/starfusion/download/environment.yml
+++ b/modules/local/starfusion/download/environment.yml
@@ -2,9 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::dfam=3.7
-  - bioconda::hmmer=3.4
-  - bioconda::star-fusion=1.7.0
-  - bioconda::trinity=2.15.2
-  - bioconda::samtools=1.21
-  - bioconda::star=2.7.11b
+  - bioconda::star-fusion=1.14.0
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index d06684869..735764c9a 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -2,7 +2,7 @@ process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
     conda "${moduleDir}/environment.yml"
-    container 'community.wave.seqera.io/library/dfam_hmmer_samtools_star-fusion_pruned:5694d82381bf039e'
+    container 'docker.io/trinityctat/starfusion:1.14.0'
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference
diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 4886a7bb7..baf2ee267 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -44,14 +44,14 @@ workflow BUILD_REFERENCES {
     GATK4_CREATESEQUENCEDICTIONARY(ENSEMBL_DOWNLOAD.out.primary_assembly)
 
 
-    RRNATRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf.map{ meta, gtf -> [ gtf ] })
+    RRNATRANSCRIPTS(ENSEMBL_DOWNLOAD.out.gtf.map{ it -> [ it[1] ] })
     BEDOPS_CONVERT2BED(RRNATRANSCRIPTS.out.rrna_gtf.map{ it -> [[id:it.Name], it] })
     GATK4_BEDTOINTERVALLIST(BEDOPS_CONVERT2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict)
 
-    GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ENSEMBL_DOWNLOAD.out.primary_assembly.map { meta, fasta -> [ fasta ] })
+    GFFREAD(ENSEMBL_DOWNLOAD.out.gtf, ENSEMBL_DOWNLOAD.out.primary_assembly.map { it -> [ it[1] ] })
 
     if (!params.skip_salmon_index){
-        SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+        SALMON_INDEX(ENSEMBL_DOWNLOAD.out.primary_assembly.map{ it -> [ it[1] ] }, GFFREAD.out.gffread_fasta.map{ it -> [ it[1] ] })
     }
 
     if (params.starindex || params.all || params.starfusion || params.arriba) {
@@ -75,9 +75,9 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.starfusion_build){
-        UCSC_GTFTOGENEPRED(ENSEMBL_DOWNLOAD.out.chrgtf)
+        UCSC_GTFTOGENEPRED(ENSEMBL_DOWNLOAD.out.gtf)
     } else {
-        UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.chrgtf)
+        UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.gtf)
     }
     if (params.fusionreport || params.all) {
         FUSIONREPORT_DOWNLOAD()

From 081249ad824b0a89aeecba37a16dcbc58b00c308 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Dec 2024 15:45:24 +0100
Subject: [PATCH 222/887] update changelog

---
 CHANGELOG.md | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a5c881c3f..7816df5ac 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,7 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `FUSIONREPORT_DOWNLOAD` [#560](https://github.com/nf-core/rnafusion/pull/560)
 - Add nf-test to local subworkflow: `QC_WORKFLOW` [#568](https://github.com/nf-core/rnafusion/pull/568)
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
-- Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#572](https://github.com/nf-core/rnafusion/pull/577)
+- Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#577](https://github.com/nf-core/rnafusion/pull/577)
 - Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
 
 ### Changed
@@ -29,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
 - Remove double nested folder introduced in [#577](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
+- Use docker.io instead of wave as to keep supporting singularity in the transition period [#588](https://github.com/nf-core/rnafusion/pull/588)
 
 ### Fixed
 

From 33f4b57edf548d98fb05b7279dd2f8aab7a57d10 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Dec 2024 16:04:26 +0100
Subject: [PATCH 223/887] revert change

---
 workflows/build_references.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index baf2ee267..9f7ce6333 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -55,7 +55,7 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.starindex || params.all || params.starfusion || params.arriba) {
-        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
+        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.chrgtf )
     }
 
     if (params.arriba || params.all) {
@@ -68,7 +68,7 @@ workflow BUILD_REFERENCES {
 
     if (params.starfusion || params.all) {
         if (params.starfusion_build){
-            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
+            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.chrgtf )
         } else {
             STARFUSION_DOWNLOAD()
         }

From ff69a60b84d2e4ff2c635e571f78fc08aec77979 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Dec 2024 16:06:40 +0100
Subject: [PATCH 224/887] revert change

---
 workflows/build_references.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/workflows/build_references.nf b/workflows/build_references.nf
index 9f7ce6333..3b88a47f9 100644
--- a/workflows/build_references.nf
+++ b/workflows/build_references.nf
@@ -55,7 +55,7 @@ workflow BUILD_REFERENCES {
     }
 
     if (params.starindex || params.all || params.starfusion || params.arriba) {
-        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.chrgtf )
+        STAR_GENOMEGENERATE( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
     }
 
     if (params.arriba || params.all) {
@@ -68,16 +68,16 @@ workflow BUILD_REFERENCES {
 
     if (params.starfusion || params.all) {
         if (params.starfusion_build){
-            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.chrgtf )
+            STARFUSION_BUILD( ENSEMBL_DOWNLOAD.out.primary_assembly, ENSEMBL_DOWNLOAD.out.gtf )
         } else {
             STARFUSION_DOWNLOAD()
         }
     }
 
     if (params.starfusion_build){
-        UCSC_GTFTOGENEPRED(ENSEMBL_DOWNLOAD.out.gtf)
+        UCSC_GTFTOGENEPRED(ENSEMBL_DOWNLOAD.out.chrgtf)
     } else {
-        UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.gtf)
+        UCSC_GTFTOGENEPRED(STARFUSION_DOWNLOAD.out.chrgtf)
     }
     if (params.fusionreport || params.all) {
         FUSIONREPORT_DOWNLOAD()

From 0db19d9301c69e4435c5c6ab9d3409a7165f0628 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 17 Dec 2024 16:11:53 +0100
Subject: [PATCH 225/887] make ctatsplicing possible with arriba input

---
 conf/modules.config                        | 16 +++++++++--
 nextflow_schema.json                       |  2 +-
 subworkflows/local/arriba_workflow/main.nf | 33 +++++++++++++++-------
 subworkflows/local/starfusion_workflow.nf  | 22 ++++++++-------
 workflows/rnafusion.nf                     | 11 ++------
 5 files changed, 52 insertions(+), 32 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index ed6bc1eba..253d0bf03 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -49,13 +49,25 @@ process {
         ]
     }
 
-    withName: 'CTATSPLICING_STARTOCANCERINTRONS' {
+    withName: '.*ARRIBA_WORKFLOW:.*:CTATSPLICING_STARTOCANCERINTRONS' {
         ext.args = {[
             bam ? "--vis" : "",
             "--sample_name ${meta.id}",
         ].join(" ")}
         publishDir = [
-            path: { "${params.outdir}/ctatsplicing" },
+            path: { "${params.outdir}/ctatsplicing/arriba" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
+    withName: '.*STARFUSION_WORKFLOW:.*:CTATSPLICING_STARTOCANCERINTRONS' {
+        ext.args = {[
+            bam ? "--vis" : "",
+            "--sample_name ${meta.id}",
+        ].join(" ")}
+        publishDir = [
+            path: { "${params.outdir}/ctatsplicing/starfusion" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
diff --git a/nextflow_schema.json b/nextflow_schema.json
index c8618561b..f7855d957 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -146,7 +146,7 @@
                 "ctatsplicing": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
-                    "description": "Build or run arriba CTAT-SPLICING"
+                    "description": "Run CTAT-splicing to detect abberant cancer splicing introns. Needs --arriba and/or --starfusion to run."
                 },
                 "ensembl_ref": {
                     "type": "string",
diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 5f59916a2..5f99ef8e6 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -4,20 +4,24 @@ include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA   } from '../../../modules/n
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_ARRIBA   } from '../../../modules/nf-core/samtools/view/main'
 include { STAR_ALIGN as STAR_FOR_ARRIBA               } from '../../../modules/nf-core/star/align/main'
 
+include { CTATSPLICING_WORKFLOW         }   from '../ctatsplicing_workflow'
+
 workflow ARRIBA_WORKFLOW {
     take:
-        reads                           // channel [ meta, [ fastqs ]         ]
-        ch_gtf                          // channel [ meta, path_gtf           ]
-        ch_fasta                        // channel [ meta, path_fasta         ]
-        ch_starindex_ref                // channel [ meta, path_index         ]
-        ch_arriba_ref_blacklist         // channel [ meta, path_blacklist     ]
-        ch_arriba_ref_known_fusions     // channel [ meta, path_known_fusions ]
-        ch_arriba_ref_cytobands         // channel [ meta, path_cytobands     ]
-        ch_arriba_ref_protein_domains   // channel [ meta, path_proteins      ]
+        reads                           // channel [ meta, [ fastqs ]          ]
+        ch_gtf                          // channel [ meta, path_gtf            ]
+        ch_fasta                        // channel [ meta, path_fasta          ]
+        ch_starindex_ref                // channel [ meta, path_index          ]
+        ch_arriba_ref_blacklist         // channel [ meta, path_blacklist      ]
+        ch_arriba_ref_known_fusions     // channel [ meta, path_known_fusions  ]
+        ch_arriba_ref_cytobands         // channel [ meta, path_cytobands      ]
+        ch_arriba_ref_protein_domains   // channel [ meta, path_proteins       ]
+        ch_starfusion_ref               // channel [ meta, path_starfusion_ref ]
         arriba                          // boolean
         all                             // boolean
         fusioninspector_only            // boolean
         star_ignore_sjdbgtf             // boolean
+        ctatsplicing                    // boolean
         seq_center                      // string
         arriba_fusions                  // path
         cram                            // array
@@ -25,7 +29,7 @@ workflow ARRIBA_WORKFLOW {
     main:
         ch_versions   = Channel.empty()
         ch_cram_index = Channel.empty()
-        ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
+        ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (( arriba || all ) && !fusioninspector_only) {
 
@@ -37,9 +41,18 @@ workflow ARRIBA_WORKFLOW {
                 '',
                 seq_center
             )
-
             ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
 
+            if ( ctatsplicing || all ) {
+                CTATSPLICING_WORKFLOW(
+                    STAR_FOR_ARRIBA.out.spl_junc_tab,
+                    STAR_FOR_ARRIBA.out.junction,
+                    STAR_FOR_ARRIBA.out.bam_sorted_aligned,
+                    ch_starfusion_ref
+                )
+                ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
+            }
+
             if ( arriba_fusions ) {
 
                 ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 8a9340da0..2a086ce1d 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -4,6 +4,8 @@ include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_STARFUSION }        from '../../mod
 include { STAR_ALIGN as STAR_FOR_STARFUSION }                    from '../../modules/nf-core/star/align/main'
 include { STARFUSION }                                           from '../../modules/local/starfusion/detect/main'
 
+include { CTATSPLICING_WORKFLOW         }   from './ctatsplicing_workflow'
+
 workflow STARFUSION_WORKFLOW {
     take:
         reads
@@ -17,13 +19,10 @@ workflow STARFUSION_WORKFLOW {
         def ch_align = Channel.empty()
         def ch_starfusion_fusions = Channel.empty()
         def bam_sorted_indexed = Channel.empty()
-        def ch_bam_align_sorted = Channel.empty()
-        def ch_split_junctions = Channel.empty()
-        def ch_junctions = Channel.empty()
 
         ch_dummy_file = file("$baseDir/assets/dummy_file_starfusion.txt", checkIfExists: true)
 
-        if ((params.starfusion || params.all || params.stringtie || params.ctatsplicing) && !params.fusioninspector_only) {
+        if ((params.starfusion || params.all || params.stringtie) && !params.fusioninspector_only) {
             if (params.starfusion_fusions){
                 ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
                                         .map { meta, reads, fusions -> [ meta, fusions ] }
@@ -37,9 +36,15 @@ workflow STARFUSION_WORKFLOW {
                 bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai)
                 reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
 
-                ch_bam_align_sorted = STAR_FOR_STARFUSION.out.bam_sorted_aligned
-                ch_split_junctions = STAR_FOR_STARFUSION.out.spl_junc_tab
-                ch_junctions = STAR_FOR_STARFUSION.out.junction
+                if (params.ctatsplicing || params.all) {
+                    CTATSPLICING_WORKFLOW(
+                        STAR_FOR_STARFUSION.out.spl_junc_tab,
+                        STAR_FOR_STARFUSION.out.junction,
+                        STAR_FOR_STARFUSION.out.bam_sorted_aligned,
+                        ch_starfusion_ref
+                    )
+                    ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
+                }
 
                 if (params.cram.contains('starfusion')){
                     SAMTOOLS_VIEW_FOR_STARFUSION (bam_sorted_indexed, ch_fasta, [] )
@@ -70,9 +75,6 @@ workflow STARFUSION_WORKFLOW {
         star_gene_count       = ch_star_gene_count
         ch_bam_sorted         = ch_align.ifEmpty([[],[]])
         ch_bam_sorted_indexed = bam_sorted_indexed.ifEmpty([[],[],[]])
-        bam_align_sorted      = ch_bam_align_sorted
-        split_junctions       = ch_split_junctions
-        junctions             = ch_junctions
         versions              = ch_versions
     }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2f1bbfab2..74315e844 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -6,7 +6,6 @@
 include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow'
 include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
-include { CTATSPLICING_WORKFLOW         }   from '../subworkflows/local/ctatsplicing_workflow'
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
@@ -100,10 +99,12 @@ workflow RNAFUSION {
         ch_arriba_ref_known_fusions,
         ch_arriba_ref_cytobands,
         ch_arriba_ref_protein_domains,
+        ch_starfusion_ref,
         params.arriba,                   // boolean
         params.all,                      // boolean
         params.fusioninspector_only,     // boolean
         params.star_ignore_sjdbgtf,      // boolean
+        params.ctatsplicing,             // boolean
         params.seq_center ?: '',         // string
         params.arriba_fusions,           // path
         params.cram                      // array
@@ -147,14 +148,6 @@ workflow RNAFUSION {
     )
     ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
 
-    //Run CTAT-splicing
-    CTATSPLICING_WORKFLOW(
-        STARFUSION_WORKFLOW.out.split_junctions,
-        STARFUSION_WORKFLOW.out.junctions,
-        STARFUSION_WORKFLOW.out.bam_align_sorted,
-        ch_starfusion_ref
-    )
-
     //Run fusionInpector
     FUSIONINSPECTOR_WORKFLOW (
         ch_reads_all,

From b7383ebaf52c8a157e365c251e6e120f37746e71 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Dec 2024 17:08:06 +0100
Subject: [PATCH 226/887] test to see if dependencies are working with
 starfusion1.14 onluy

---
 modules/local/fusioninspector/main.nf     | 4 +++-
 modules/local/starfusion/build/main.nf    | 4 +++-
 modules/local/starfusion/detect/main.nf   | 4 +++-
 modules/local/starfusion/download/main.nf | 4 +++-
 4 files changed, 12 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index b91d37c9c..8745b0e3a 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -3,7 +3,9 @@ process FUSIONINSPECTOR {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'trinityctat/fusioninspector:2.10.0'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
+        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 9212cdafa..15b5517a1 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -2,7 +2,9 @@ process STARFUSION_BUILD {
     tag 'star-fusion'
 
     conda "${moduleDir}/environment.yml"
-    container 'docker.io/trinityctat/starfusion:1.14.0'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
+        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 94342e99c..38a881123 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -3,7 +3,9 @@ process STARFUSION {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'docker.io/trinityctat/starfusion:1.14.0'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
+        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 735764c9a..6defb6cdd 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -2,7 +2,9 @@ process STARFUSION_DOWNLOAD {
     tag 'star-fusion'
 
     conda "${moduleDir}/environment.yml"
-    container 'docker.io/trinityctat/starfusion:1.14.0'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
+        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference

From 17ef23354947b4965c84131f9eb927bc323d1cbc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 17 Dec 2024 17:49:22 +0100
Subject: [PATCH 227/887] use custom container for fusioncatcher, fix typo

---
 modules/local/fusioncatcher/detect/main.nf   |   3 +-
 modules/local/fusioncatcher/download/main.nf |   2 +-
 tests/test_stub.nf.test.snap                 | 198 ++-----------------
 workflows/rnafusion.nf                       |   4 +-
 4 files changed, 19 insertions(+), 188 deletions(-)

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 9a0928463..e753ff850 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -3,7 +3,8 @@ process FUSIONCATCHER {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
+
+    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 03c0a1f3c..e04c24f6e 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -3,7 +3,7 @@ process FUSIONCATCHER_DOWNLOAD {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f"
+    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
 
 
     input:
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 83c76dd72..e3a8bb73e 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,11 +1,8 @@
 {
     "stub test no fastp trim": {
         "content": [
-            31,
+            20,
             {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
@@ -19,14 +16,11 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER": {
-                    "fusioncatcher": 1.33
+                    "fusioncatcher": 1.35
                 },
                 "FUSIONCATCHER_BUILD": {
                     "fusioncatcher": "fusioncatcher.py 1.35"
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
-                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "2.1.5"
                 },
@@ -36,10 +30,6 @@
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
-                },
                 "GENCODE_DOWNLOAD": {
                     "wget": null
                 },
@@ -55,59 +45,32 @@
                 "HGNC_DOWNLOAD": {
                     "wget": null
                 },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SALMON_INDEX": {
-                    "salmon": "1.10.3"
-                },
-                "SALMON_QUANT": {
-                    "salmon": "1.10.3"
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "SAMTOOLS_INDEX_FOR_STARFUSION": {
-                    "samtools": 1.21
-                },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.7.0"
-                },
                 "STARFUSION_BUILD": {
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": "2.7.11b",
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": "2.7.11b",
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.3"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -126,32 +89,11 @@
                 "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
                 "fusioncatcher/test.fusioncatcher.log",
                 "fusioncatcher/test.fusioncatcher.summary.txt",
-                "fusionreport",
-                "fusionreport/test",
-                "fusionreport/test/AAA_BBB.html",
-                "fusionreport/test/test.fusionreport.tsv",
-                "fusionreport/test/test.fusionreport_filtered.tsv",
-                "fusionreport/test/test.fusions.csv",
-                "fusionreport/test/test.fusions.json",
-                "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "get",
                 "get/rrna.bed",
                 "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
                 "references",
@@ -214,10 +156,6 @@
                 "references/starfusion",
                 "references/starfusion/ctat_genome_lib_build_dir",
                 "references/starfusion/ref_annot.cdna.fa",
-                "salmon",
-                "salmon/test",
-                "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
                 "star_for_arriba",
                 "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
                 "star_for_arriba/test.Aligned.unsort.out.bam",
@@ -249,38 +187,23 @@
                 "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
                 "star_for_starfusion/test.out.sam",
                 "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
                 "star_for_starfusion/test.tab",
                 "star_for_starfusion/test.toTranscriptome.out.bam",
                 "star_for_starfusion/test.unmapped_1.fastq.gz",
                 "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv",
-                "stringtie",
-                "stringtie/[:]",
-                "stringtie/[:]/stringtie.merged.gtf",
-                "stringtie/test.ballgown",
-                "stringtie/test.coverage.gtf",
-                "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
+                "star_for_starfusion/testXd.out.bam"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-11T12:43:59.534889"
+        "timestamp": "2024-12-17T16:44:02.121512"
     },
     "stub test with fastp trim": {
         "content": [
-            31,
+            19,
             {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
@@ -294,14 +217,11 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER": {
-                    "fusioncatcher": 1.33
+                    "fusioncatcher": 1.35
                 },
                 "FUSIONCATCHER_BUILD": {
                     "fusioncatcher": "fusioncatcher.py 1.35"
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
-                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "2.1.5"
                 },
@@ -311,10 +231,6 @@
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
-                },
                 "GENCODE_DOWNLOAD": {
                     "wget": null
                 },
@@ -330,59 +246,27 @@
                 "HGNC_DOWNLOAD": {
                     "wget": null
                 },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SALMON_INDEX": {
-                    "salmon": "1.10.3"
-                },
-                "SALMON_QUANT": {
-                    "salmon": "1.10.3"
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "SAMTOOLS_INDEX_FOR_STARFUSION": {
-                    "samtools": 1.21
-                },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.7.0"
-                },
                 "STARFUSION_BUILD": {
                     "STAR-Fusion": "1.7.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": "2.7.11b",
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "STAR_FOR_STARFUSION": {
-                    "star": "2.7.11b",
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
+                    "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.3"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -401,32 +285,11 @@
                 "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
                 "fusioncatcher/test.fusioncatcher.log",
                 "fusioncatcher/test.fusioncatcher.summary.txt",
-                "fusionreport",
-                "fusionreport/test",
-                "fusionreport/test/AAA_BBB.html",
-                "fusionreport/test/test.fusionreport.tsv",
-                "fusionreport/test/test.fusionreport_filtered.tsv",
-                "fusionreport/test/test.fusions.csv",
-                "fusionreport/test/test.fusions.json",
-                "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "get",
                 "get/rrna.bed",
                 "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
                 "references",
@@ -452,23 +315,6 @@
                 "references/hgnc",
                 "references/hgnc/HGNC-DB-timestamp.txt",
                 "references/hgnc/hgnc_complete_set.txt",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
                 "references/star",
                 "references/star/Genome",
                 "references/star/Log.out",
@@ -489,10 +335,6 @@
                 "references/starfusion",
                 "references/starfusion/ctat_genome_lib_build_dir",
                 "references/starfusion/ref_annot.cdna.fa",
-                "salmon",
-                "salmon/test",
-                "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
                 "star_for_arriba",
                 "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
                 "star_for_arriba/test.Aligned.unsort.out.bam",
@@ -524,29 +366,17 @@
                 "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
                 "star_for_starfusion/test.out.sam",
                 "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
                 "star_for_starfusion/test.tab",
                 "star_for_starfusion/test.toTranscriptome.out.bam",
                 "star_for_starfusion/test.unmapped_1.fastq.gz",
                 "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv",
-                "stringtie",
-                "stringtie/[:]",
-                "stringtie/[:]/stringtie.merged.gtf",
-                "stringtie/test.ballgown",
-                "stringtie/test.coverage.gtf",
-                "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
+                "star_for_starfusion/testXd.out.bam"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-11T12:40:22.066494"
+        "timestamp": "2024-12-17T16:42:11.182735"
     }
-}
+}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index d992bf58d..91742e1bc 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -90,7 +90,7 @@ workflow RNAFUSION {
         BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
         BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
         BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
-        BUILD_REFERENCES.out.ch_arriba_ref_protein_domains
+        BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
         params.arriba,                   // boolean
         params.all,                      // boolean
         params.fusioninspector_only,     // boolean
@@ -139,7 +139,7 @@ workflow RNAFUSION {
 
 
 
-    //Run fusionInpector
+    //Run fusionInspector
     FUSIONINSPECTOR_WORKFLOW (
         ch_reads,
         FUSIONREPORT_WORKFLOW.out.fusion_list,

From a67cc0f6f14d6905f9d18a861ef82fef4e08975d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Dec 2024 09:51:42 +0100
Subject: [PATCH 228/887] add dependencies explicitly

---
 modules/local/starfusion/build/environment.yml  | 6 ++++++
 modules/local/starfusion/build/main.nf          | 4 ++--
 modules/local/starfusion/detect/environment.yml | 6 ++++++
 modules/local/starfusion/detect/main.nf         | 4 ++--
 4 files changed, 16 insertions(+), 4 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 77187e8bf..eacee484a 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -2,4 +2,10 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::minimap2=2.28
+  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
+  - bioconda::star=2.7.11a
+  - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 15b5517a1..05ca30895 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,8 +3,8 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
-        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f8/f804c086cee338c315fbc51faf0c37de360b047b47c33388716962eb3467b087/data':
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index 77187e8bf..eacee484a 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -2,4 +2,10 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::minimap2=2.28
+  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
+  - bioconda::star=2.7.11a
+  - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 38a881123..84a57d21f 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,8 +4,8 @@ process STARFUSION {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
-        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f8/f804c086cee338c315fbc51faf0c37de360b047b47c33388716962eb3467b087/data':
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
 
     input:
     tuple val(meta), path(reads), path(junction)

From bf05cb766954979dd741a315c2018585df9fb5d0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Dec 2024 14:52:16 +0100
Subject: [PATCH 229/887] update bin path

---
 modules/local/starfusion/build/main.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 05ca30895..47b881c00 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -14,7 +14,6 @@ process STARFUSION_BUILD {
     path "*"  , emit: reference
 
     script:
-    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
     """
     export TMPDIR=/tmp
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
@@ -26,7 +25,7 @@ process STARFUSION_BUILD {
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-    $binPath \\
+    prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --annot_filter_rule AnnotFilterRule.pm \\

From 235a7529b6e93db0d2553a86805a7a14c3ba9761 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Dec 2024 15:47:49 +0100
Subject: [PATCH 230/887] bin path to prep_genome_lib not working with
 singularity wave container, reverting to galaxy and specifying path

---
 modules/local/starfusion/build/main.nf  | 5 +++--
 modules/local/starfusion/detect/main.nf | 2 +-
 2 files changed, 4 insertions(+), 3 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 47b881c00..38d278326 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,7 +3,7 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f8/f804c086cee338c315fbc51faf0c37de360b047b47c33388716962eb3467b087/data':
+        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
         'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
 
     input:
@@ -14,6 +14,7 @@ process STARFUSION_BUILD {
     path "*"  , emit: reference
 
     script:
+    def binPath = (workflow.profile.tokenize(',').intersect(['singularity']).size() >= 1)  ?  "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl" : "prep_genome_lib.pl"
     """
     export TMPDIR=/tmp
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
@@ -25,7 +26,7 @@ process STARFUSION_BUILD {
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-    prep_genome_lib.pl \\
+    $binPath \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --annot_filter_rule AnnotFilterRule.pm \\
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 84a57d21f..c95e39265 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,7 +4,7 @@ process STARFUSION {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f8/f804c086cee338c315fbc51faf0c37de360b047b47c33388716962eb3467b087/data':
+        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
         'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
 
     input:

From a0698b1f08e0643283e6a4bb80633098aca7e408 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Dec 2024 16:17:21 +0100
Subject: [PATCH 231/887] propagate container of starfusion to fusioninspector

---
 modules/local/fusioninspector/environment.yml     | 6 ++++++
 modules/local/fusioninspector/main.nf             | 4 ++--
 modules/local/starfusion/build/environment.yml    | 2 +-
 modules/local/starfusion/detect/environment.yml   | 2 +-
 modules/local/starfusion/download/environment.yml | 6 ++++++
 modules/local/starfusion/download/main.nf         | 4 ++--
 6 files changed, 18 insertions(+), 6 deletions(-)

diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index 77187e8bf..757544ca1 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -2,4 +2,10 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::minimap2=2.28
+  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
+  - bioconda::star=2.7.9
+  - bioconda::trinity=2.8.5
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 8745b0e3a..53a0bb45e 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
-        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
+        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index eacee484a..757544ca1 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -7,5 +7,5 @@ dependencies:
   - bioconda::minimap2=2.28
   - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.11a
+  - bioconda::star=2.7.9
   - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index eacee484a..757544ca1 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -7,5 +7,5 @@ dependencies:
   - bioconda::minimap2=2.28
   - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.11a
+  - bioconda::star=2.7.9
   - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/download/environment.yml b/modules/local/starfusion/download/environment.yml
index 77187e8bf..757544ca1 100644
--- a/modules/local/starfusion/download/environment.yml
+++ b/modules/local/starfusion/download/environment.yml
@@ -2,4 +2,10 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
+  - bioconda::dfam=3.7
+  - bioconda::hmmer=3.4
+  - bioconda::minimap2=2.28
+  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
+  - bioconda::star=2.7.9
+  - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 6defb6cdd..19cad44bc 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -3,8 +3,8 @@ process STARFUSION_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f0/f0bf770bcc7a7af4c9b7a83a88a191bb9e9875759e1985030268df9ffa27dde6/data':
-        'community.wave.seqera.io/library/star-fusion:4d0a3a362520dfa6'}"
+        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference

From b2a075bc0e0f43ac296685b605e2f56e0322265f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Dec 2024 16:19:47 +0100
Subject: [PATCH 232/887] update star

---
 modules/local/fusioninspector/environment.yml     | 2 +-
 modules/local/starfusion/build/environment.yml    | 2 +-
 modules/local/starfusion/detect/environment.yml   | 2 +-
 modules/local/starfusion/download/environment.yml | 2 +-
 4 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index 757544ca1..f5d21d5a8 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -7,5 +7,5 @@ dependencies:
   - bioconda::minimap2=2.28
   - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.9
+  - bioconda::star=2.7.11b
   - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 757544ca1..f5d21d5a8 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -7,5 +7,5 @@ dependencies:
   - bioconda::minimap2=2.28
   - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.9
+  - bioconda::star=2.7.11b
   - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index 757544ca1..f5d21d5a8 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -7,5 +7,5 @@ dependencies:
   - bioconda::minimap2=2.28
   - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.9
+  - bioconda::star=2.7.11b
   - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/download/environment.yml b/modules/local/starfusion/download/environment.yml
index 757544ca1..f5d21d5a8 100644
--- a/modules/local/starfusion/download/environment.yml
+++ b/modules/local/starfusion/download/environment.yml
@@ -7,5 +7,5 @@ dependencies:
   - bioconda::minimap2=2.28
   - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.9
+  - bioconda::star=2.7.11b
   - bioconda::trinity=2.8.5

From 853e48d9e9ca66aac81fe1ba6d78f65f599802bc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Dec 2024 16:22:22 +0100
Subject: [PATCH 233/887] update changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7816df5ac..b281d82f0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -29,7 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Remove local module `RRNA_TRANSCRIPTS` (replaced by nf-core module) [#541](https://github.com/nf-core/rnafusion/pull/541)
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
 - Remove double nested folder introduced in [#577](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
-- Use docker.io instead of wave as to keep supporting singularity in the transition period [#588](https://github.com/nf-core/rnafusion/pull/588)
+- Use docker.io and galaxy containers for fusioncatcher and starfusion (incl. fusioninspector) instead of wave as they are not functional on wave [#588](https://github.com/nf-core/rnafusion/pull/588)
 
 ### Fixed
 

From 9cad032e4a706c2a79dca951a6361a348bff2f1d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 18 Dec 2024 16:23:45 +0100
Subject: [PATCH 234/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index b281d82f0..1d400fa2e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -30,6 +30,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Allow fastq files without a dot before .fn(.gz)/.fastq(.gz) files [#548](https://github.com/nf-core/rnafusion/pull/548)
 - Remove double nested folder introduced in [#577](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
 - Use docker.io and galaxy containers for fusioncatcher and starfusion (incl. fusioninspector) instead of wave as they are not functional on wave [#588](https://github.com/nf-core/rnafusion/pull/588)
+- Update STAR-Fusion to 1.14 [#588](https://github.com/nf-core/rnafusion/pull/588)
 
 ### Fixed
 

From f2b5ed59d611a9340225a1e436c4bf6ad35d393f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 18 Dec 2024 16:14:20 +0000
Subject: [PATCH 235/887] fix: update starfusion container declaration to
 latest nf-core standards

---
 modules/local/starfusion/build/main.nf | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 484389b12..becd98d7c 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,7 +3,9 @@ process STARFUSION_BUILD {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/09/0941210949303990f23c63831c7f23ceec099d52ffbe5f1ac2152964d328747e/data':
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086' }"
 
     input:
     tuple val(meta), path(fasta)

From 8556d5cb0d74ba74c13930caf6e00c1732d4f977 Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Wed, 18 Dec 2024 21:46:58 +0100
Subject: [PATCH 236/887] updated test

---
 modules/local/fusioncatcher/detect/main.nf    |  3 +-
 .../local/fusioncatcher_workflow/main.nf      | 17 ++--
 .../fusioncatcher_workflow/tests/main.nf.test |  3 +-
 .../tests/main.nf.test.snap                   |  6 +-
 tests/nextflow.config                         |  2 +-
 tests/test_build.nf.test.snap                 | 47 +++-------
 tests/test_cosmic.nf.test.snap                | 94 +++++++++++++++++++
 workflows/rnafusion.nf                        |  8 +-
 8 files changed, 132 insertions(+), 48 deletions(-)
 create mode 100644 tests/test_cosmic.nf.test.snap

diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index c7ba7bfb4..0fef68aa9 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -1,4 +1,4 @@
-process FUSIONCATCHER {
+process FUSIONCATCHER_DETECT {
     tag "$meta.id"
     label 'process_high'
 
@@ -21,7 +21,6 @@ process FUSIONCATCHER {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def reads = fastqs.toString().replace(" ", ",")
     def single_end = meta.single_end ? "--single-end" : ""
     """
     fusioncatcher.py \\
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index 3bf7c9e9a..fd9ce34b9 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -1,4 +1,9 @@
-include { FUSIONCATCHER } from '../../../modules/local/fusioncatcher/detect/main'
+include { FUSIONCATCHER_DETECT } from '../../../modules/local/fusioncatcher/detect/main'
+
+// TODO: Remove fusioncatcher_fusions as parameter.
+// TODO: remove dummy file. Work with Channel.empty()
+// TODO: if the files were already produced and the user want to skip the module because of this, they should be taken them from the sample sheet
+// TODO: harmonize `run_fusioncatcher` and `fusioncatcher_only` parameters at main workflow level to activate/skip this one.
 
 workflow FUSIONCATCHER_WORKFLOW {
     take:
@@ -20,12 +25,12 @@ workflow FUSIONCATCHER_WORKFLOW {
                                             .map { meta, reads, fusions -> [ meta, fusions ] }
             } else {
 
-                FUSIONCATCHER (
+                FUSIONCATCHER_DETECT (
                     reads,
                     fusioncatcher_ref
                 )
-                ch_fusioncatcher_fusions = FUSIONCATCHER.out.fusions
-                ch_versions = ch_versions.mix(FUSIONCATCHER.out.versions)
+                ch_fusioncatcher_fusions = FUSIONCATCHER_DETECT.out.fusions
+                ch_versions              = ch_versions.mix(FUSIONCATCHER_DETECT.out.versions)
             }
         }
         else {
@@ -34,7 +39,7 @@ workflow FUSIONCATCHER_WORKFLOW {
         }
 
     emit:
-        fusions  = ch_fusioncatcher_fusions
-        versions = ch_versions
+        fusions  = ch_fusioncatcher_fusions     // channel [ meta, fusions ]
+        versions = ch_versions                  // channel [ versions      ]
     }
 
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index 89d2f78ad..d864b252c 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -21,6 +21,7 @@ nextflow_workflow {
                 """
                 }
             }
+        }
         
         when {
             workflow {
@@ -36,7 +37,7 @@ nextflow_workflow {
                     ] )
 
                 // ch_references
-                input[1] = Channel.fromPath("/scratch/moebbs/NEXTFLOW_CODE/reference_rnafusion/human_v102").map{ [ [id: "human_v102"], it] } //FUSIONCATCHER_DOWNLOAD.out.reference
+                input[1] = FUSIONCATCHER_DOWNLOAD.out.reference 
 
                 // fusioncatcher (boolean)
                 input[2] = true
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
index 522bc131b..ae57fe642 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -12,7 +12,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,38af82f57bcacbd86c21109a3d425e62"
+                    "versions.yml:md5,6462518e02774293fa221cead8373305"
                 ],
                 "fusions": [
                     [
@@ -24,7 +24,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,38af82f57bcacbd86c21109a3d425e62"
+                    "versions.yml:md5,6462518e02774293fa221cead8373305"
                 ]
             }
         ],
@@ -32,6 +32,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.0"
         },
-        "timestamp": "2024-12-11T19:59:17.754866"
+        "timestamp": "2024-12-18T21:26:47.477736"
     }
 }
\ No newline at end of file
diff --git a/tests/nextflow.config b/tests/nextflow.config
index c3f050dcb..abb1ba9c1 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -3,6 +3,6 @@ process {
     resourceLimits = [
         cpus: 4,
         memory: '15.GB',
-        time: '1.h'
+        time: '4.h'
     ]
 }
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 16b6c0079..ec588ad23 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -2,46 +2,25 @@
     "test_build": {
         "content": [
             [
-                "bedops",
-                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
-                "gffread",
-                "gffread/Homo_sapiens.GRCh38.102.fasta",
+                "fastqc",
+                "fastqc/test_1_fastqc.html",
+                "fastqc/test_1_fastqc.zip",
+                "fastqc/test_2_fastqc.html",
+                "fastqc/test_2_fastqc.zip",
                 "pipeline_info",
-                "references",
-                "references/ensembl",
-                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
-                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
-                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
-                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
-                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
-                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "rrnatranscripts",
-                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
             ],
             [
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,5bc3ccc76735ae46699a75269f0ea65b",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,092f1bf29fce906ff7b5ece02b4b21c8",
-                "Homo_sapiens.GRCh38.102.fasta:md5,d1f17b045dc60c49f2cc29e30006afc0",
-                "Homo_sapiens.GRCh38.102.chr.gtf:md5,0069687307852c63596b4d2ebdbbaf0c",
-                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d527f3eb6b664020cf4d882b5820056f",
-                "Homo_sapiens.GRCh38.102.genepred:md5,59c577ca4ab033c0ce1feac1e387bcab",
-                "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
-                "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,0abf61877f65247b15c438d605d85599",
-                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
-                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,744bf505deb50837b15441e808cad345"
+                "test_1_fastqc.html:md5,fce82f01594d12b109776ced37ec0739",
+                "test_1_fastqc.zip:md5,fd0eae421abf76c38f60656bc0fcf5cf",
+                "test_2_fastqc.html:md5,40f5f42c62a0e36def4fc3d3c48089cd",
+                "test_2_fastqc.zip:md5,1aaf903d6ea3957abe6f03ad832f82fa"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.0"
         },
-        "timestamp": "2024-12-09T13:33:28.517098377"
+        "timestamp": "2024-12-18T21:05:30.032816"
     }
 }
\ No newline at end of file
diff --git a/tests/test_cosmic.nf.test.snap b/tests/test_cosmic.nf.test.snap
new file mode 100644
index 000000000..fa74fd3b5
--- /dev/null
+++ b/tests/test_cosmic.nf.test.snap
@@ -0,0 +1,94 @@
+{
+    "test cosmic no fastp trim": {
+        "content": [
+            1,
+            {
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "fastqc",
+                "fastqc/test_1_fastqc.html",
+                "fastqc/test_1_fastqc.zip",
+                "fastqc/test_2_fastqc.html",
+                "fastqc/test_2_fastqc.zip",
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+            ],
+            [
+                "test_1_fastqc.html:md5,fce82f01594d12b109776ced37ec0739",
+                "test_1_fastqc.zip:md5,166d13bbf5ebfcb24eb30c78b129a172",
+                "test_2_fastqc.html:md5,40f5f42c62a0e36def4fc3d3c48089cd",
+                "test_2_fastqc.zip:md5,4747768318b29ce4effe6c572ccbc36c"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.0"
+        },
+        "timestamp": "2024-12-18T21:06:31.911972"
+    },
+    "test cosmic with fastp trim": {
+        "content": [
+            3,
+            {
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test_1_fastqc.html",
+                "fastqc/test_1_fastqc.zip",
+                "fastqc/test_2_fastqc.html",
+                "fastqc/test_2_fastqc.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed_1_fastqc.html",
+                "fastqc_for_fastp/test_trimmed_1_fastqc.zip",
+                "fastqc_for_fastp/test_trimmed_2_fastqc.html",
+                "fastqc_for_fastp/test_trimmed_2_fastqc.zip",
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+            ],
+            [
+                "test.fastp.html:md5,d26a7f988fd5ed667832d391ab06e202",
+                "test.fastp.json:md5,ebf549d5793aa40efcd9e1ca41504f26",
+                "test.fastp.log:md5,1c04b14e212aac5ace51f9bd25859f23",
+                "test_1.fastp.fastq.gz:md5,2a5519a03fb226ae2379b1150b02ff0b",
+                "test_2.fastp.fastq.gz:md5,4b8d3865b857e6bfae0035a45b2cdc27",
+                "test_1_fastqc.html:md5,fce82f01594d12b109776ced37ec0739",
+                "test_1_fastqc.zip:md5,f9e7e4dbf71703d98214cfd5cb79ee07",
+                "test_2_fastqc.html:md5,40f5f42c62a0e36def4fc3d3c48089cd",
+                "test_2_fastqc.zip:md5,7428e5d3c26f0934c75b15de29d6af5c",
+                "test_trimmed_1_fastqc.html:md5,2c2256226962e9be8c20e5720b8a51ab",
+                "test_trimmed_1_fastqc.zip:md5,3b8731204e07bfe7a6ecce66ba8617db",
+                "test_trimmed_2_fastqc.html:md5,343c493ba215089723e9d4df7180a0fc",
+                "test_trimmed_2_fastqc.zip:md5,fcec86b3067854ffdadd00db615f0cdd"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.0"
+        },
+        "timestamp": "2024-12-18T21:06:00.758494"
+    }
+}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bafea68c3..3beb599f1 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -108,8 +108,14 @@ workflow RNAFUSION {
 
 
     //Run fusioncatcher
+    // TODO: check this inputs!
     FUSIONCATCHER_WORKFLOW (
-        ch_reads_fusioncatcher
+        ch_reads_fusioncatcher,
+        params.fusioncatcher_ref,      // channel [ meta, path       ]
+        params.run_fusioncatcher,
+        params.all,
+        params.fusioninspector_only,
+        params.fusioncatcher_fusions
     )
     ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
 

From 350de1b443139e8e38634fc03438f2f3e243876e Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 10:08:36 +0100
Subject: [PATCH 237/887] add some views to debug the full run

---
 subworkflows/local/fusionreport_workflow.nf | 12 +++++++++---
 1 file changed, 9 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index 09ec99657..a0673f64e 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -15,10 +15,16 @@ workflow FUSIONREPORT_WORKFLOW {
         ch_csv = Channel.empty()
 
         if (!params.fusioninspector_only) {
+            arriba_fusions.view { it -> "arriba fusions:${it}"}
+            starfusion_fusions.view { it -> "starfusion fusions:${it}"}
+            fusioncatcher_fusions.view { it -> "fusioncatcher fusions:${it}"}
+
             reads_fusions = reads
-            .join(arriba_fusions, remainder: true)
-            .join(starfusion_fusions, remainder: true)
-            .join(fusioncatcher_fusions, remainder: true)
+            .join(arriba_fusions, failOnMismatch:true, failOnDuplicate:true)
+            .join(starfusion_fusions, failOnMismatch:true, failOnDuplicate:true)
+            .join(fusioncatcher_fusions, failOnMismatch:true, failOnDuplicate:true)
+
+            reads_fusions.view()
 
             FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
             ch_fusion_list = FUSIONREPORT.out.fusion_list

From a08c02f1d0f7d900e841fe4643d628f0bab8b71e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Dec 2024 11:19:41 +0100
Subject: [PATCH 238/887] update dependencies

---
 modules/local/starfusion/build/environment.yml    | 4 ----
 modules/local/starfusion/build/main.nf            | 4 ++--
 modules/local/starfusion/detect/environment.yml   | 4 ----
 modules/local/starfusion/detect/main.nf           | 4 ++--
 modules/local/starfusion/download/environment.yml | 4 ----
 modules/local/starfusion/download/main.nf         | 4 ++--
 6 files changed, 6 insertions(+), 18 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index f5d21d5a8..18b2407bd 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -4,8 +4,4 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.28
-  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.11b
-  - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 38d278326..eba6e2c0b 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,8 +3,8 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f7/f7977acd75883433483258a1cc21d069a64947af431b26c3fc13c6be62666dfa/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_star-fusion:e8fe96707386872f'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index f5d21d5a8..18b2407bd 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -4,8 +4,4 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.28
-  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.11b
-  - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index c95e39265..691601e99 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,8 +4,8 @@ process STARFUSION {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f7/f7977acd75883433483258a1cc21d069a64947af431b26c3fc13c6be62666dfa/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_star-fusion:e8fe96707386872f'}"
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/environment.yml b/modules/local/starfusion/download/environment.yml
index f5d21d5a8..18b2407bd 100644
--- a/modules/local/starfusion/download/environment.yml
+++ b/modules/local/starfusion/download/environment.yml
@@ -4,8 +4,4 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.28
-  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.11b
-  - bioconda::trinity=2.8.5
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 19cad44bc..7dffbf2c0 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -3,8 +3,8 @@ process STARFUSION_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f7/f7977acd75883433483258a1cc21d069a64947af431b26c3fc13c6be62666dfa/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_star-fusion:e8fe96707386872f'}"
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference

From 34109cd9ecd754793cf6fdc419e1fcc60dbe1cda Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Dec 2024 11:35:02 +0100
Subject: [PATCH 239/887] update path again

---
 modules/local/starfusion/build/main.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index eba6e2c0b..e106bf801 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -14,7 +14,6 @@ process STARFUSION_BUILD {
     path "*"  , emit: reference
 
     script:
-    def binPath = (workflow.profile.tokenize(',').intersect(['singularity']).size() >= 1)  ?  "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl" : "prep_genome_lib.pl"
     """
     export TMPDIR=/tmp
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
@@ -26,7 +25,7 @@ process STARFUSION_BUILD {
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_3.4/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p --no-check-certificate
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-    $binPath \\
+    prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --annot_filter_rule AnnotFilterRule.pm \\

From 0ea316e94661b472b7e615e8d0233a3690ed6f5a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Dec 2024 11:57:02 +0100
Subject: [PATCH 240/887] add minimap

---
 modules/local/starfusion/build/environment.yml    | 1 +
 modules/local/starfusion/build/main.nf            | 2 +-
 modules/local/starfusion/detect/environment.yml   | 1 +
 modules/local/starfusion/download/environment.yml | 1 +
 4 files changed, 4 insertions(+), 1 deletion(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 18b2407bd..ef7f93160 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -4,4 +4,5 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
+  - bioconda::minimap2=2.28
   - bioconda::star-fusion=1.14.0
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index e106bf801..ad35b0674 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,7 +3,7 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f7/f7977acd75883433483258a1cc21d069a64947af431b26c3fc13c6be62666dfa/data' :
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
         'community.wave.seqera.io/library/dfam_hmmer_star-fusion:e8fe96707386872f'}"
 
     input:
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index 18b2407bd..ef7f93160 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -4,4 +4,5 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
+  - bioconda::minimap2=2.28
   - bioconda::star-fusion=1.14.0
diff --git a/modules/local/starfusion/download/environment.yml b/modules/local/starfusion/download/environment.yml
index 18b2407bd..ef7f93160 100644
--- a/modules/local/starfusion/download/environment.yml
+++ b/modules/local/starfusion/download/environment.yml
@@ -4,4 +4,5 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
+  - bioconda::minimap2=2.28
   - bioconda::star-fusion=1.14.0

From 27b3f8b3f198f18ba725ecb3ef6bf82bf42000ed Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Dec 2024 12:48:30 +0100
Subject: [PATCH 241/887] propagate container/env change

---
 modules/local/fusioninspector/environment.yml | 3 ---
 modules/local/fusioninspector/main.nf         | 4 ++--
 modules/local/starfusion/build/main.nf        | 2 +-
 modules/local/starfusion/detect/main.nf       | 4 ++--
 modules/local/starfusion/download/main.nf     | 4 ++--
 5 files changed, 7 insertions(+), 10 deletions(-)

diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index f5d21d5a8..ef7f93160 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -5,7 +5,4 @@ dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
   - bioconda::minimap2=2.28
-  - bioconda::samtools=1.6
   - bioconda::star-fusion=1.14.0
-  - bioconda::star=2.7.11b
-  - bioconda::trinity=2.8.5
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 53a0bb45e..ade7c174a 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/star-fusion:1.14.0--hdfd78af_0' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_samtools_pruned:bd39df228dad7086'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index ad35b0674..05abec921 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -4,7 +4,7 @@ process STARFUSION_BUILD {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_star-fusion:e8fe96707386872f'}"
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 691601e99..83b0dc2f9 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,8 +4,8 @@ process STARFUSION {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f7/f7977acd75883433483258a1cc21d069a64947af431b26c3fc13c6be62666dfa/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_star-fusion:e8fe96707386872f'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
index 7dffbf2c0..989c24209 100644
--- a/modules/local/starfusion/download/main.nf
+++ b/modules/local/starfusion/download/main.nf
@@ -3,8 +3,8 @@ process STARFUSION_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f7/f7977acd75883433483258a1cc21d069a64947af431b26c3fc13c6be62666dfa/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_star-fusion:e8fe96707386872f'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
 
     output:
     path "ctat_genome_lib_build_dir/*"            , emit: reference

From b8bae77aef45119ebb922bb2fba11ca6b017a8f4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 14:59:21 +0100
Subject: [PATCH 242/887] try to fix issue with arriba output

---
 subworkflows/local/arriba_workflow/main.nf | 7 ++++---
 1 file changed, 4 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 5f99ef8e6..949e35ffd 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -27,9 +27,10 @@ workflow ARRIBA_WORKFLOW {
         cram                            // array
 
     main:
-        ch_versions   = Channel.empty()
-        ch_cram_index = Channel.empty()
-        ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
+        def ch_versions   = Channel.empty()
+        def ch_cram_index = Channel.empty()
+        def ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
+        def ch_arriba_fusions = Channel.empty()
 
         if (( arriba || all ) && !fusioninspector_only) {
 

From 00673c6af1d920721ff2177dff5fb13af444f52f Mon Sep 17 00:00:00 2001
From: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 11:14:31 -0300
Subject: [PATCH 243/887] Update conf/modules.config

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 5e5e0a014..f241f879e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -329,7 +329,7 @@ process {
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
-        ext.args = "--max_readlength ${params.read_length}"
+        ext.args = "--max_readlength ${params.read_length} --human_gencode_filter"
     }
 
     withName: 'STARFUSION_DOWNLOAD' {

From 00063944fa9f6623855ebbee272bcfba5f08a331 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 15:18:05 +0100
Subject: [PATCH 244/887] remove views

---
 subworkflows/local/fusionreport_workflow.nf | 6 ------
 1 file changed, 6 deletions(-)

diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow.nf
index a0673f64e..6a87244fd 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow.nf
@@ -15,17 +15,11 @@ workflow FUSIONREPORT_WORKFLOW {
         ch_csv = Channel.empty()
 
         if (!params.fusioninspector_only) {
-            arriba_fusions.view { it -> "arriba fusions:${it}"}
-            starfusion_fusions.view { it -> "starfusion fusions:${it}"}
-            fusioncatcher_fusions.view { it -> "fusioncatcher fusions:${it}"}
-
             reads_fusions = reads
             .join(arriba_fusions, failOnMismatch:true, failOnDuplicate:true)
             .join(starfusion_fusions, failOnMismatch:true, failOnDuplicate:true)
             .join(fusioncatcher_fusions, failOnMismatch:true, failOnDuplicate:true)
 
-            reads_fusions.view()
-
             FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
             ch_fusion_list = FUSIONREPORT.out.fusion_list
             ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered

From 05e0e61110137b1684c3b661e603196bf6ea82f9 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 15:20:29 +0100
Subject: [PATCH 245/887] remove breaking ifEmpties

---
 subworkflows/local/starfusion_workflow.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 2a086ce1d..74c1839e0 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -73,8 +73,8 @@ workflow STARFUSION_WORKFLOW {
         fusions               = ch_starfusion_fusions
         star_stats            = ch_star_stats
         star_gene_count       = ch_star_gene_count
-        ch_bam_sorted         = ch_align.ifEmpty([[],[]])
-        ch_bam_sorted_indexed = bam_sorted_indexed.ifEmpty([[],[],[]])
+        ch_bam_sorted         = ch_align
+        ch_bam_sorted_indexed = bam_sorted_indexed
         versions              = ch_versions
     }
 

From f8dd5169afb714f6f823ba64a3a58099ba695407 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Dec 2024 15:27:34 +0100
Subject: [PATCH 246/887] update changelog

---
 CHANGELOG.md | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a52f01f4f..33c5b0cc1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,9 +31,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Remove double nested folder introduced in [#577](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
 - Use docker.io and galaxy containers for fusioncatcher and starfusion (incl. fusioninspector) instead of wave as they are not functional on wave [#588](https://github.com/nf-core/rnafusion/pull/588)
 - Update STAR-Fusion to 1.14 [#588](https://github.com/nf-core/rnafusion/pull/588)
-
-  ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons" GTF_TO_REFFLAT
-
+- Use -genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons for GTF_TO_REFFLAT[#505](https://github.com/nf-core/rnafusion/pull/505)
 
 ### Fixed
 

From 62fdf33845012d6c24993e105aec82e6318cda8a Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 15:50:33 +0100
Subject: [PATCH 247/887] remove def

---
 modules/nf-core/star/align/main.nf         | 3 ++-
 subworkflows/local/arriba_workflow/main.nf | 3 +--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index b5bc9ddf8..18a3b461f 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -41,7 +41,8 @@ process STAR_ALIGN {
     script:
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
-    def reads1 = [], reads2 = []
+    def reads1 = []
+    def reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
     def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""
diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 949e35ffd..c7089e857 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -30,7 +30,6 @@ workflow ARRIBA_WORKFLOW {
         def ch_versions   = Channel.empty()
         def ch_cram_index = Channel.empty()
         def ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
-        def ch_arriba_fusions = Channel.empty()
 
         if (( arriba || all ) && !fusioninspector_only) {
 
@@ -48,7 +47,7 @@ workflow ARRIBA_WORKFLOW {
                 CTATSPLICING_WORKFLOW(
                     STAR_FOR_ARRIBA.out.spl_junc_tab,
                     STAR_FOR_ARRIBA.out.junction,
-                    STAR_FOR_ARRIBA.out.bam_sorted_aligned,
+                    STAR_FOR_ARRIBA.out.bam,
                     ch_starfusion_ref
                 )
                 ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)

From d62c4b5d1f3e691497956dfe77574cc3f7c95dc7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Dec 2024 15:54:27 +0100
Subject: [PATCH 248/887] update changelog, retrofit trim_workflowo

---
 CHANGELOG.md                             | 17 +++++++++++++----
 subworkflows/local/trim_workflow/main.nf |  6 ++++--
 workflows/rnafusion.nf                   |  4 +++-
 3 files changed, 20 insertions(+), 7 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 33c5b0cc1..51fef20e4 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,6 +21,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
 - Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#577](https://github.com/nf-core/rnafusion/pull/577)
 - Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
+- Add parameter `--references_only` when no data should be analysed, but only the references should be built [#505](https://github.com/nf-core/rnafusion/pull/505)
 
 ### Changed
 
@@ -31,7 +32,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Remove double nested folder introduced in [#577](https://github.com/nf-core/rnafusion/pull/577), [#581](https://github.com/nf-core/rnafusion/pull/581)
 - Use docker.io and galaxy containers for fusioncatcher and starfusion (incl. fusioninspector) instead of wave as they are not functional on wave [#588](https://github.com/nf-core/rnafusion/pull/588)
 - Update STAR-Fusion to 1.14 [#588](https://github.com/nf-core/rnafusion/pull/588)
-- Use -genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons for GTF_TO_REFFLAT[#505](https://github.com/nf-core/rnafusion/pull/505)
+- Use "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons" (to mimic gms/tomte) for GTF_TO_REFFLAT [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Integrate reference building in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Move from ensembl to gencode base [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Update from ensembl 102 to gencode 46 default references [#505](https://github.com/nf-core/rnafusion/pull/505)
 
 ### Fixed
 
@@ -46,12 +50,17 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Removed
 
 - Remove fusionGDB from documentation and fusion-report download stubs [#503](https://github.com/nf-core/rnafusion/pull/503)
+- Removed test-build as reference building gets integrated in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Removed parameter `--build_references`
+
 
 ### Parameters
 
-| Old parameter | New parameter |
-| ------------- | ------------- |
-|               | `--no_cosmic` |
+| Old parameter       | New parameter |
+| ------------------- | ------------------- |
+|                     | `--no_cosmic`       |
+| `-build_references` | `--references_only` |
+
 
 ## v3.0.2 - [2024-04-10]
 
diff --git a/subworkflows/local/trim_workflow/main.nf b/subworkflows/local/trim_workflow/main.nf
index 512a07b54..d548c8aec 100644
--- a/subworkflows/local/trim_workflow/main.nf
+++ b/subworkflows/local/trim_workflow/main.nf
@@ -7,6 +7,8 @@ workflow TRIM_WORKFLOW {
 
     take:
         reads           // channel [ meta, [ fastq files ] ]
+        adapter_fasta   // channel [ path ]
+        fastp_trim      // boolean
 
     main:
         ch_versions       = Channel.empty()
@@ -14,8 +16,8 @@ workflow TRIM_WORKFLOW {
         ch_fastp_json     = Channel.empty()
         ch_fastqc_trimmed = Channel.empty()
 
-        if ( {params.fastp_trim} ) {
-            FASTP(reads, {params.adapter_fasta}, false, false, false)
+        if ( fastp_trim ) {
+            FASTP(reads, adapter_fasta.ifEmpty( [] ), false, false, false)
             ch_versions = ch_versions.mix(FASTP.out.versions)
 
             FASTQC_FOR_FASTP(FASTP.out.reads)
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 91742e1bc..a755311c4 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -64,7 +64,9 @@ workflow RNAFUSION {
     // Trimming
     //
     TRIM_WORKFLOW (
-        ch_samplesheet
+        ch_samplesheet,
+        params.adapter_fasta,
+        params.fastp_trim
     )
     ch_reads = TRIM_WORKFLOW.out.ch_reads_all
     ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)

From 8155168298873fdfc04f9c99fd68a179e2c40b4f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 16:03:01 +0100
Subject: [PATCH 249/887] redo star changes

---
 modules/nf-core/star/align/main.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index 18a3b461f..b5bc9ddf8 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -41,8 +41,7 @@ process STAR_ALIGN {
     script:
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
-    def reads1 = []
-    def reads2 = []
+    def reads1 = [], reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
     def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""

From e386c81f7d8d948da0db6aa509202ca61297bf86 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 16:05:12 +0100
Subject: [PATCH 250/887] use bam from starfusion

---
 subworkflows/local/starfusion_workflow.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index 74c1839e0..b0c72bfcb 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -40,7 +40,7 @@ workflow STARFUSION_WORKFLOW {
                     CTATSPLICING_WORKFLOW(
                         STAR_FOR_STARFUSION.out.spl_junc_tab,
                         STAR_FOR_STARFUSION.out.junction,
-                        STAR_FOR_STARFUSION.out.bam_sorted_aligned,
+                        STAR_FOR_STARFUSION.out.bam,
                         ch_starfusion_ref
                     )
                     ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)

From 2c01b3c2311069aa9fc91a50518be77d8526863e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Dec 2024 16:07:15 +0100
Subject: [PATCH 251/887] use placeholder and add TODO for update

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 32ccc1d0e..a7ab06b9d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -30,7 +30,7 @@ params {
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
-    fusion_annot_lib           = null       // path to  dat.gz CTAT genome lib (e.g. https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v44_CTAT_lib_Oct292023.plug-n-play.tar.gz)
+    fusion_annot_lib           = 'https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v44_CTAT_lib_Oct292023.plug-n-play.tar.gz)' //TODO: update to latest version
 
     // Genomes options
     fasta          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"

From 62ee76f8c38849ccf13e715c544e8ddb4a94030b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 16:13:19 +0100
Subject: [PATCH 252/887] create junctions with arriba for ctatsplicing

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 253d0bf03..b19d935b1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -291,7 +291,7 @@ process {
         --alignSplicedMateMapLminOverLmate 0.5 \
         --alignSJstitchMismatchNmax 5 -1 5 5 \
         --chimSegmentMin 10 \
-        --chimOutType WithinBAM HardClip \
+        --chimOutType WithinBAM HardClip Junctions \
         --chimJunctionOverhangMin 10 \
         --chimScoreDropMax 30 \
         --chimScoreJunctionNonGTAG 0 \

From 19d1cc19651476042a96834d2c125842768ab11c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 19 Dec 2024 17:15:46 +0100
Subject: [PATCH 253/887] do not create an index in ctat splicing

---
 modules/local/ctatsplicing/startocancerintrons/main.nf | 2 --
 1 file changed, 2 deletions(-)

diff --git a/modules/local/ctatsplicing/startocancerintrons/main.nf b/modules/local/ctatsplicing/startocancerintrons/main.nf
index 30fd5c3f2..edd9878fd 100644
--- a/modules/local/ctatsplicing/startocancerintrons/main.nf
+++ b/modules/local/ctatsplicing/startocancerintrons/main.nf
@@ -29,9 +29,7 @@ process CTATSPLICING_STARTOCANCERINTRONS {
     prefix = task.ext.prefix ?: "${meta.id}"
     def bam_arg = bam ? "--bam_file ${bam}" : ""
     def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    def create_index = bam && !bai ? "samtools index ${bam}" : ""
     """
-    ${create_index}
     /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \\
         --SJ_tab_file ${split_junction} \\
         --chimJ_file ${junction} \\

From 1237fefd30a47b82ac614d278a5f11291173d80c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 16:42:58 +0000
Subject: [PATCH 254/887] fix: add full path to module to avoid changing
 nf-tests

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index f241f879e..dcad2f40a 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -320,7 +320,7 @@ process {
         ]
     }
 
-    withName: 'STARFUSION_BUILD' {
+    withName: 'NFCORE_RNAFUSION:BUILD_REFERENCES:STARFUSION_BUILD' {
         cpus   = { 24    * task.attempt }
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }

From 89b26494a46658a17f099b2c1f9fc30bcf54dc52 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 16:43:36 +0000
Subject: [PATCH 255/887] feat: use previous `.dat.gz` file as default

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 32ccc1d0e..fabbbdbad 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -30,7 +30,7 @@ params {
     read_length                = 100
     starfusion_build           = true
     genomes                    = [:]
-    fusion_annot_lib           = null       // path to  dat.gz CTAT genome lib (e.g. https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v44_CTAT_lib_Oct292023.plug-n-play.tar.gz)
+    fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
 
     // Genomes options
     fasta          = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa"

From fb64ca6e557ca548eac8fce398c893b1f9c85ec6 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 17:21:47 +0000
Subject: [PATCH 256/887] test: update snapshot

---
 .../local/starfusion/build/tests/main.nf.test.snap | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/modules/local/starfusion/build/tests/main.nf.test.snap b/modules/local/starfusion/build/tests/main.nf.test.snap
index e50db541e..b08f25197 100644
--- a/modules/local/starfusion/build/tests/main.nf.test.snap
+++ b/modules/local/starfusion/build/tests/main.nf.test.snap
@@ -62,12 +62,12 @@
             "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
             "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
             "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-            "Genome:md5,21aac51c5062f73d6305ae77691f5d4e",
+            "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
             true,
             true,
-            "SA:md5,4d6ee7c82328aa304300e51ab5677ff9",
-            "SAindex:md5,2d3498f7d84a69bbc65fe9de1887820c",
-            "sjdbInfo.txt:md5,0ad0c108e6d02ec541d6adeffbdea6fd",
+            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
             "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
             "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
             "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
@@ -77,10 +77,10 @@
             null
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-12T14:57:13.528134845"
+        "timestamp": "2024-12-19T17:03:12.812884291"
     },
     "STARFUSION_BUILD - human - minigenome - stub": {
         "content": [

From 970fded943572aed0d74b285de9263188c445c1c Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Thu, 19 Dec 2024 18:22:01 +0100
Subject: [PATCH 257/887] update snaps

---
 .../fusioncatcher_workflow/tests/main.nf.test |  8 +-
 .../tests/main.nf.test.snap                   |  2 +-
 tests/test_build.nf.test.snap                 | 15 +---
 tests/test_cosmic.nf.test.snap                | 77 +++++++++++++++----
 4 files changed, 71 insertions(+), 31 deletions(-)

diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index d864b252c..d0304380a 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -11,7 +11,7 @@ nextflow_workflow {
     // Test
     test("FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4") {
 
-        setup {
+        /*setup {
             // Download reference files for fusioncatch
             run("FUSIONCATCHER_DOWNLOAD") {
                 script "../../../../modules/local/fusioncatcher/download/main.nf"
@@ -21,8 +21,9 @@ nextflow_workflow {
                 """
                 }
             }
-        }
+        }*/
         
+        // TODO: get smaller reference files for fusioncatcher
         when {
             workflow {
                 """
@@ -37,7 +38,8 @@ nextflow_workflow {
                     ] )
 
                 // ch_references
-                input[1] = FUSIONCATCHER_DOWNLOAD.out.reference 
+                input[1] = Channel.fromPath("complete/with/your/local/path", checkIfExists: true)
+                    .map{ [[id: "human_v102" ], it] } //FUSIONCATCHER_DOWNLOAD.out.reference 
 
                 // fusioncatcher (boolean)
                 input[2] = true
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
index ae57fe642..523916e4b 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -32,6 +32,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.0"
         },
-        "timestamp": "2024-12-18T21:26:47.477736"
+        "timestamp": "2024-12-19T17:44:38.284199"
     }
 }
\ No newline at end of file
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index ec588ad23..b278711ac 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -2,25 +2,16 @@
     "test_build": {
         "content": [
             [
-                "fastqc",
-                "fastqc/test_1_fastqc.html",
-                "fastqc/test_1_fastqc.zip",
-                "fastqc/test_2_fastqc.html",
-                "fastqc/test_2_fastqc.zip",
-                "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+                
             ],
             [
-                "test_1_fastqc.html:md5,fce82f01594d12b109776ced37ec0739",
-                "test_1_fastqc.zip:md5,fd0eae421abf76c38f60656bc0fcf5cf",
-                "test_2_fastqc.html:md5,40f5f42c62a0e36def4fc3d3c48089cd",
-                "test_2_fastqc.zip:md5,1aaf903d6ea3957abe6f03ad832f82fa"
+                
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.0"
         },
-        "timestamp": "2024-12-18T21:05:30.032816"
+        "timestamp": "2024-12-19T12:50:27.263029"
     }
 }
\ No newline at end of file
diff --git a/tests/test_cosmic.nf.test.snap b/tests/test_cosmic.nf.test.snap
index fa74fd3b5..4ff01a919 100644
--- a/tests/test_cosmic.nf.test.snap
+++ b/tests/test_cosmic.nf.test.snap
@@ -1,4 +1,51 @@
 {
+    "test cosmic no fastp trim build": {
+        "content": [
+            9,
+            null,
+            [
+                "bedops",
+                "bedops/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.102.dna.primary_assembly.dict",
+                "gffread",
+                "gffread/Homo_sapiens.GRCh38.102.fasta",
+                "pipeline_info",
+                "references",
+                "references/ensembl",
+                "references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa",
+                "references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai",
+                "references/ensembl/Homo_sapiens.GRCh38.102.genepred",
+                "references/ensembl/Homo_sapiens.GRCh38.102.gtf",
+                "references/ensembl/Homo_sapiens.GRCh38.102.refflat",
+                "references/ensembl/Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "rrnatranscripts",
+                "rrnatranscripts/Homo_sapiens.GRCh38.102_rrna_intervals.gtf"
+            ],
+            [
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.bed:md5,5bc3ccc76735ae46699a75269f0ea65b",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.dict:md5,092f1bf29fce906ff7b5ece02b4b21c8",
+                "Homo_sapiens.GRCh38.102.fasta:md5,d1f17b045dc60c49f2cc29e30006afc0",
+                "Homo_sapiens.GRCh38.102.chr.gtf:md5,0069687307852c63596b4d2ebdbbaf0c",
+                "Homo_sapiens.GRCh38.102.dna.primary_assembly.fa.fai:md5,d527f3eb6b664020cf4d882b5820056f",
+                "Homo_sapiens.GRCh38.102.genepred:md5,59c577ca4ab033c0ce1feac1e387bcab",
+                "Homo_sapiens.GRCh38.102.gtf:md5,defac755cd9aa4e82ec33398c27745ef",
+                "Homo_sapiens.GRCh38.102.refflat:md5,ef095e13743811c31d44752c32e9673e",
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf.interval_list:md5,0abf61877f65247b15c438d605d85599",
+                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
+                "Homo_sapiens.GRCh38.102_rrna_intervals.gtf:md5,744bf505deb50837b15441e808cad345"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.0"
+        },
+        "timestamp": "2024-12-19T14:45:08.191019"
+    },
     "test cosmic no fastp trim": {
         "content": [
             1,
@@ -20,17 +67,17 @@
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
             ],
             [
-                "test_1_fastqc.html:md5,fce82f01594d12b109776ced37ec0739",
-                "test_1_fastqc.zip:md5,166d13bbf5ebfcb24eb30c78b129a172",
-                "test_2_fastqc.html:md5,40f5f42c62a0e36def4fc3d3c48089cd",
-                "test_2_fastqc.zip:md5,4747768318b29ce4effe6c572ccbc36c"
+                "test_1_fastqc.html:md5,413a6e28e6dd182616fb5859822e288e",
+                "test_1_fastqc.zip:md5,793d81ea4349817b15ff37f6b053f870",
+                "test_2_fastqc.html:md5,820795d75b698565195c6a20c492c9fb",
+                "test_2_fastqc.zip:md5,179dee147c92fed9b552a2f2f82b7367"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.0"
         },
-        "timestamp": "2024-12-18T21:06:31.911972"
+        "timestamp": "2024-12-19T12:51:54.89817"
     },
     "test cosmic with fastp trim": {
         "content": [
@@ -70,25 +117,25 @@
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
             ],
             [
-                "test.fastp.html:md5,d26a7f988fd5ed667832d391ab06e202",
+                "test.fastp.html:md5,4a1627af1a54b4f7b2dcf43c34369f04",
                 "test.fastp.json:md5,ebf549d5793aa40efcd9e1ca41504f26",
                 "test.fastp.log:md5,1c04b14e212aac5ace51f9bd25859f23",
                 "test_1.fastp.fastq.gz:md5,2a5519a03fb226ae2379b1150b02ff0b",
                 "test_2.fastp.fastq.gz:md5,4b8d3865b857e6bfae0035a45b2cdc27",
-                "test_1_fastqc.html:md5,fce82f01594d12b109776ced37ec0739",
-                "test_1_fastqc.zip:md5,f9e7e4dbf71703d98214cfd5cb79ee07",
-                "test_2_fastqc.html:md5,40f5f42c62a0e36def4fc3d3c48089cd",
-                "test_2_fastqc.zip:md5,7428e5d3c26f0934c75b15de29d6af5c",
-                "test_trimmed_1_fastqc.html:md5,2c2256226962e9be8c20e5720b8a51ab",
-                "test_trimmed_1_fastqc.zip:md5,3b8731204e07bfe7a6ecce66ba8617db",
-                "test_trimmed_2_fastqc.html:md5,343c493ba215089723e9d4df7180a0fc",
-                "test_trimmed_2_fastqc.zip:md5,fcec86b3067854ffdadd00db615f0cdd"
+                "test_1_fastqc.html:md5,413a6e28e6dd182616fb5859822e288e",
+                "test_1_fastqc.zip:md5,f4c865fd4ae054f2a98ae971ffdef576",
+                "test_2_fastqc.html:md5,820795d75b698565195c6a20c492c9fb",
+                "test_2_fastqc.zip:md5,67466d294966125f9e4e11333a231080",
+                "test_trimmed_1_fastqc.html:md5,6919dace995f86210c983eff554c43e7",
+                "test_trimmed_1_fastqc.zip:md5,069fd1c49854f8aea29acf5d870fa9cf",
+                "test_trimmed_2_fastqc.html:md5,6b567bc53367bb126896d7aa27e0c6f4",
+                "test_trimmed_2_fastqc.zip:md5,a65ef7a18c69fb6d8b3d0a2f9f87d938"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.0"
         },
-        "timestamp": "2024-12-18T21:06:00.758494"
+        "timestamp": "2024-12-19T12:51:19.311692"
     }
 }
\ No newline at end of file

From dea8445b8c1fc98a1187a713d47b83df5416bbe7 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 18:57:01 +0000
Subject: [PATCH 258/887] feat: allow to use either reads or junction files

---
 modules/local/starfusion/detect/main.nf   | 4 ++--
 subworkflows/local/starfusion_workflow.nf | 2 +-
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 6ab7e9b81..12c4fd945 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -19,8 +19,8 @@ process STARFUSION {
 
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def fasta = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
-    def junction_arg = junction ? "-J ${junction}": ""
+    def fasta = reads ? (meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}") : ""
+    def junction_arg = !reads && junction ? "-J ${junction}" : ""
     def args = task.ext.args ?: ''
     """
     STAR-Fusion \\
diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index de99d8e51..e0e58c8bb 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -30,7 +30,7 @@ workflow STARFUSION_WORKFLOW {
                 SAMTOOLS_INDEX_FOR_STARFUSION(STAR_FOR_STARFUSION.out.bam_sorted)
                 ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION.out.versions)
                 bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai)
-                reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )
+                reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )  // TODO: This join is not needed as STARFUSION can simply read from the junction file: https://github.com/STAR-Fusion/STAR-Fusion/wiki#alternatively-kickstart-mode-running-star-yourself-and-then-running-star-fusion-using-the-existing-outputs
 
                 if (params.cram.contains('starfusion')){
                     SAMTOOLS_VIEW_FOR_STARFUSION (bam_sorted_indexed, ch_fasta, [] )

From 9aff4ea4e1574e69780c1396dfba65139197359f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 18:57:08 +0000
Subject: [PATCH 259/887] test: update snaps

---
 .../starfusion/detect/tests/main.nf.test.snap | 20 +++++++++----------
 1 file changed, 10 insertions(+), 10 deletions(-)

diff --git a/modules/local/starfusion/detect/tests/main.nf.test.snap b/modules/local/starfusion/detect/tests/main.nf.test.snap
index 2532b33d6..f65f94b02 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test.snap
+++ b/modules/local/starfusion/detect/tests/main.nf.test.snap
@@ -30,7 +30,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,4131f39f483637d95446231588add8f1"
+                    "versions.yml:md5,a8fb6344fdf740dde0941048313fc243"
                 ],
                 "abridged": [
                     [
@@ -60,15 +60,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,4131f39f483637d95446231588add8f1"
+                    "versions.yml:md5,a8fb6344fdf740dde0941048313fc243"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.2"
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-16T14:47:21.862981618"
+        "timestamp": "2024-12-19T18:42:06.988178092"
     },
     "Should run without failures": {
         "content": [
@@ -78,7 +78,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.starfusion.fusion_predictions.tsv:md5,2adb2aeb44ea98736fb7572e7b5de5c6"
+                    "test.starfusion.fusion_predictions.tsv:md5,82834fffed743afe07da82bd56d50c99"
                 ]
             ],
             [
@@ -87,7 +87,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.starfusion.abridged.tsv:md5,f37f1cd07a3a6ff41e131dbe369bc093"
+                    "test.starfusion.abridged.tsv:md5,d6d20fdd4b5cba21b9c0ebf8e0ea19ff"
                 ]
             ],
             [
@@ -96,17 +96,17 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.starfusion.abridged.coding_effect.tsv:md5,8f5739b0351a725ee96cdf52860d680e"
+                    "test.starfusion.abridged.coding_effect.tsv:md5,95dfce6fdaf3589f23881fe1e855c62b"
                 ]
             ],
             [
-                "versions.yml:md5,4131f39f483637d95446231588add8f1"
+                "versions.yml:md5,a8fb6344fdf740dde0941048313fc243"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.2"
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-16T14:47:06.459753683"
+        "timestamp": "2024-12-19T18:41:49.150362156"
     }
 }
\ No newline at end of file

From a369518bae364760580be681ce46b45192e28beb Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 19:08:46 +0000
Subject: [PATCH 260/887] docs: update changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index b1cfe3071..25152acae 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,7 +22,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#577](https://github.com/nf-core/rnafusion/pull/577)
 - Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
 - Add nf-test to local module: `STARFUSION_BUILD`. [#585](https://github.com/nf-core/rnafusion/pull/585)
-
+- Add nf-test to local module: `STARFUSION_DETECT`. [#586](https://github.com/nf-core/rnafusion/pull/586)
 
 ### Changed
 

From 6f2a382e9edefbbb078093722c2303961e508b14 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 19 Dec 2024 19:24:55 +0000
Subject: [PATCH 261/887] fix: add junction if provided

---
 modules/local/starfusion/detect/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 12c4fd945..fd0a7d46b 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -20,7 +20,7 @@ process STARFUSION {
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
     def fasta = reads ? (meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}") : ""
-    def junction_arg = !reads && junction ? "-J ${junction}" : ""
+    def junction_arg =  junction ? "-J ${junction}" : ""
     def args = task.ext.args ?: ''
     """
     STAR-Fusion \\

From 9dc352fb4fd95259ce6ac877fba7bcb93d093ad0 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 20 Dec 2024 08:25:55 +0100
Subject: [PATCH 262/887] readd indexing to ctat & sort arriba bam

---
 conf/modules.config                                    | 2 +-
 modules/local/ctatsplicing/startocancerintrons/main.nf | 3 +++
 2 files changed, 4 insertions(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index b19d935b1..252fede94 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -283,7 +283,7 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
         ext.args = '--readFilesCommand zcat \
-        --outSAMtype BAM Unsorted \
+        --outSAMtype BAM SortedByCoordinate \
         --outSAMunmapped Within \
         --outBAMcompression 0 \
         --outFilterMultimapNmax 50 \
diff --git a/modules/local/ctatsplicing/startocancerintrons/main.nf b/modules/local/ctatsplicing/startocancerintrons/main.nf
index edd9878fd..a8d683ec2 100644
--- a/modules/local/ctatsplicing/startocancerintrons/main.nf
+++ b/modules/local/ctatsplicing/startocancerintrons/main.nf
@@ -29,7 +29,10 @@ process CTATSPLICING_STARTOCANCERINTRONS {
     prefix = task.ext.prefix ?: "${meta.id}"
     def bam_arg = bam ? "--bam_file ${bam}" : ""
     def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    def create_index = bam && !bai ? "samtools index ${bam}" : ""
     """
+    ${create_index}
+
     /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \\
         --SJ_tab_file ${split_junction} \\
         --chimJ_file ${junction} \\

From de5d6d5aea8306d2853fef7c788f7c20f7a50ee8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 20 Dec 2024 09:57:45 +0100
Subject: [PATCH 263/887] add species as parameter

---
 CHANGELOG.md                           |   4 +-
 nextflow.config                        |   1 +
 subworkflows/local/build_references.nf |   6 +-
 tests/test_stub.nf.test.snap           | 368 +------------------------
 4 files changed, 13 insertions(+), 366 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 51fef20e4..1838af9e1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -53,15 +53,13 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed test-build as reference building gets integrated in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Removed parameter `--build_references`
 
-
 ### Parameters
 
-| Old parameter       | New parameter |
+| Old parameter       | New parameter       |
 | ------------------- | ------------------- |
 |                     | `--no_cosmic`       |
 | `-build_references` | `--references_only` |
 
-
 ## v3.0.2 - [2024-04-10]
 
 ### Added
diff --git a/nextflow.config b/nextflow.config
index a1e746dd8..a61462e3e 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -31,6 +31,7 @@ params {
     starfusion_build           = true
     genomes                    = [:]
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
+    species                    = "human"
 
     // Filtering
     tools_cutoff               = 1
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 8e9cc4b1e..56fd8eb24 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -88,9 +88,9 @@ workflow BUILD_REFERENCES {
 
     if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty() ||
         !file(params.salmon_index_stub_check).exists() || file(params.salmon_index_stub_check).isEmpty()){ // add condition for qc
-        GFFREAD(ch_gtf, ch_fasta.map{ meta, fasta -> [ fasta ] })
+        GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
         ch_versions = ch_versions.mix(GFFREAD.out.versions)
-        SALMON_INDEX(ch_fasta.map{ meta, fasta -> [ fasta ] }, GFFREAD.out.gffread_fasta.map{ meta, gffread_fasta -> [ gffread_fasta ] })
+        SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
         ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
         ch_salmon_index = SALMON_INDEX.out.index
     } else {
@@ -140,7 +140,7 @@ workflow BUILD_REFERENCES {
     if ((params.starfusion || params.all) &&
             (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
             !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
-            STARFUSION_BUILD(ch_fasta, ch_gtf)
+            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             ch_starfusion_ref = STARFUSION_BUILD.out.reference
     }
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index e3a8bb73e..f661aac6c 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,382 +1,30 @@
 {
     "stub test no fastp trim": {
         "content": [
-            20,
-            {
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
-                },
-                "FASTP": {
-                    "fastp": "0.23.4"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FASTQC_FOR_FASTP": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONCATCHER": {
-                    "fusioncatcher": 1.35
-                },
-                "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "fusioncatcher.py 1.35"
-                },
-                "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": null
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GFFREAD": {
-                    "gffread": "0.12.7"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "HGNC_DOWNLOAD": {
-                    "wget": null
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION_BUILD": {
-                    "STAR-Fusion": "1.7.0"
-                },
-                "STAR_FOR_ARRIBA": {
-                    "star": null,
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "STAR_FOR_STARFUSION": {
-                    "star": null,
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": null,
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            0,
+            null,
             [
-                "fastp",
-                "fastp/test.fastp.html",
-                "fastp/test.fastp.json",
-                "fastp/test.fastp.log",
-                "fastp/test_1.fastp.fastq.gz",
-                "fastp/test_2.fastp.fastq.gz",
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
-                "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
-                "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ref_annot.cdna.fa",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam"
+                "pipeline_info"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-17T16:44:02.121512"
+        "timestamp": "2024-12-20T09:49:41.743056"
     },
     "stub test with fastp trim": {
         "content": [
-            19,
-            {
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
-                },
-                "FASTP": {
-                    "fastp": "0.23.4"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FASTQC_FOR_FASTP": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONCATCHER": {
-                    "fusioncatcher": 1.35
-                },
-                "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "fusioncatcher.py 1.35"
-                },
-                "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": null
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GFFREAD": {
-                    "gffread": "0.12.7"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "HGNC_DOWNLOAD": {
-                    "wget": null
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION_BUILD": {
-                    "STAR-Fusion": "1.7.0"
-                },
-                "STAR_FOR_ARRIBA": {
-                    "star": null,
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": null,
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            0,
+            null,
             [
-                "fastp",
-                "fastp/test.fastp.html",
-                "fastp/test.fastp.json",
-                "fastp/test.fastp.log",
-                "fastp/test_1.fastp.fastq.gz",
-                "fastp/test_2.fastp.fastq.gz",
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
-                "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
-                "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ref_annot.cdna.fa",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam"
+                "pipeline_info"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-17T16:42:11.182735"
+        "timestamp": "2024-12-20T09:49:14.743338"
     }
 }
\ No newline at end of file

From 9ea1f3b46836c440a8868352bb47632b47709e42 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 20 Dec 2024 11:32:36 +0100
Subject: [PATCH 264/887] remove ctatsplicing check from starfusion

---
 subworkflows/local/starfusion_workflow.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf
index b0c72bfcb..2083036fc 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow.nf
@@ -53,7 +53,7 @@ workflow STARFUSION_WORKFLOW {
                     SAMTOOLS_INDEX_FOR_STARFUSION_CRAM (SAMTOOLS_VIEW_FOR_STARFUSION.out.cram)
                     ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION_CRAM.out.versions)
                 }
-                if (params.starfusion || params.all || params.ctatsplicing){
+                if (params.starfusion || params.all){
                     STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
                     ch_versions = ch_versions.mix(STARFUSION.out.versions)
                     ch_starfusion_fusions = STARFUSION.out.fusions

From 1c98fc76d7dbf9404ad8522b4c0b8c55eed2fb2f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 20 Dec 2024 11:32:43 +0100
Subject: [PATCH 265/887] clarify changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7168d02be..f09fb2222 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,7 +21,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `TRIM_WORKFLOW` [#572](https://github.com/nf-core/rnafusion/pull/572)
 - Add nf-test to local module: `FUSIONREPORT_DETECT`. Improve `FUSIONREPORT_DOWNLOAD` module [#577](https://github.com/nf-core/rnafusion/pull/577)
 - Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
-- Added a new module `CTATSPLICING_STARTOCANCERINTRONS` [#587](https://github.com/nf-core/rnafusion/pull/587)
+- Added a new module `CTATSPLICING_STARTOCANCERINTRONS` and a new parameter `--ctatsplicing`. This options creates reports on cancer splicing abberations and requires one or both of `--arriba` and `--starfusion` to be given. [#587](https://github.com/nf-core/rnafusion/pull/587)
 
 ### Changed
 

From 74d4534681116df35a81b72d4488ff346a4ed84f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 20 Dec 2024 11:40:13 +0100
Subject: [PATCH 266/887] update docs

---
 docs/output.md | 37 +++++++++++++++++++++++++++++++++++++
 docs/usage.md  |  4 ++--
 2 files changed, 39 insertions(+), 2 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 62859668a..efc1425f5 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -17,6 +17,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 - [STAR-fusion](#starfusion) - STAR-fusion fusion detection
 - [StringTie](#stringtie) - StringTie assembly
 - [FusionCatcher](#fusioncatcher) - Fusion catcher fusion detection
+- [CTAT-SPLICING](#ctat-splicing) - Detection and annotation of cancer splicing aberrations
 - [Samtools](#samtools) - SAM/BAM file manipulation
 - [Fusion-report](#fusion-report) - Summary of the findings of each tool and comparison to COSMIC, Mitelman, and FusionGDB2 databases
 - [FusionInspector](#fusionInspector) - Supervised analysis of fusion predictions from fusion-report, recover and re-score evidence for such predictions
@@ -186,6 +187,42 @@ The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They m
 
 [FusionCatcher](https://github.com/ndaniel/fusioncatcher) searches for novel/known somatic fusion genes translocations, and chimeras in RNA-seq data. Possibility to use parameter `--fusioncatcher_limitSjdbInsertNsj` to modify limitSjdbInsertNsj.
 
+### CTAT-SPLICING
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `ctatsplicing`
+  - `arriba`
+    - `<sample>.cancer_intron_reads.sorted.bam`
+    - `<sample>.cancer_intron_reads.sorted.bam.bai`
+    - `<sample>.cancer.introns`
+    - `<sample>.cancer.introns.prelim`
+    - `<sample>.chckpts`
+    - `<sample>.ctat-splicing.igv.html`
+    - `<sample>.gene_reads.sorted.sifted.bam`
+    - `<sample>.gene_reads.sorted.sifted.bam.bai`
+    - `<sample>.igv.tracks`
+    - `<sample>.introns`
+    - `<sample>.introns.for_IGV.bed`
+  - `starfusion`
+    - `<sample>.cancer_intron_reads.sorted.bam`
+    - `<sample>.cancer_intron_reads.sorted.bam.bai`
+    - `<sample>.cancer.introns`
+    - `<sample>.cancer.introns.prelim`
+    - `<sample>.chckpts`
+    - `<sample>.ctat-splicing.igv.html`
+    - `<sample>.gene_reads.sorted.sifted.bam`
+    - `<sample>.gene_reads.sorted.sifted.bam.bai`
+    - `<sample>.igv.tracks`
+    - `<sample>.introns`
+    - `<sample>.introns.for_IGV.bed`
+
+</details>
+
+[CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING/wiki) detects and annotates of aberrant splicing isoforms in cancer. This is run on the input files for `arriba` and/or `starfusion`.
+
+
 ### FusionInspector
 
 <details markdown="1">
diff --git a/docs/usage.md b/docs/usage.md
index b353b28ce..1bddcf71e 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -16,7 +16,7 @@ The pipeline is divided into two parts:
 
 2. Detecting fusions
 
-- Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, and `StringTie`
+- Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, `StringTie` and `CTAT-SPLICING`
 - QC: `Fastqc`, `MultiQC`, and `Picard CollectInsertSize`, `Picard CollectWgsMetrics`, `Picard Markduplicates`
 - Fusions visualization: `Arriba`, `fusion-report`, `FusionInspector`, and `vcf_collect`
 
@@ -136,7 +136,7 @@ As you can see above for multiple runs of the same sample, the `sample` name has
 
 ### Starting commands
 
-The pipeline can either be run using all fusion detection tools or specifying individual tools. Visualisation tools will be run on all fusions detected. To run all tools (`arriba`, `fusioncatcher`, `starfusion`, `stringtie`) use the `--all` parameter:
+The pipeline can either be run using all fusion detection tools or specifying individual tools. Visualisation tools will be run on all fusions detected. To run all tools (`arriba`, `fusioncatcher`, `starfusion`, `stringtie`, `ctat-splicing`) use the `--all` parameter:
 
 ```bash
 nextflow run nf-core/rnafusion \

From a209feaab80eb5a34428337e1b3173d4028f3452 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 20 Dec 2024 11:50:57 +0100
Subject: [PATCH 267/887] prettyyy

---
 docs/output.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index efc1425f5..dd3705751 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -222,7 +222,6 @@ The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They m
 
 [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING/wiki) detects and annotates of aberrant splicing isoforms in cancer. This is run on the input files for `arriba` and/or `starfusion`.
 
-
 ### FusionInspector
 
 <details markdown="1">

From bdae2931727b81c1b5a13d3c1deb894a84f1e07d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 20 Dec 2024 11:55:16 +0100
Subject: [PATCH 268/887] fix some issues with channels

---
 subworkflows/local/arriba_workflow/main.nf |   8 +-
 subworkflows/local/build_references.nf     |  12 +-
 tests/test_stub.nf.test.snap               | 246 ++++++++++++++++++++-
 workflows/rnafusion.nf                     |   2 +-
 4 files changed, 253 insertions(+), 15 deletions(-)

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 77e97f424..bb4c36a68 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -25,7 +25,7 @@ workflow ARRIBA_WORKFLOW {
     main:
         ch_versions   = Channel.empty()
         ch_cram_index = Channel.empty()
-        ch_dummy_file = file("$baseDir/assets/dummy_file_arriba.txt", checkIfExists: true)
+        ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (( arriba || all ) && !fusioninspector_only) {
 
@@ -43,7 +43,7 @@ workflow ARRIBA_WORKFLOW {
             if ( arriba_fusions ) {
 
                 ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
-                    .map { meta, reads, fusions -> [ meta, fusions ] }
+                    .map { it -> [ it[0], it[2] ] }
                 ch_arriba_fusion_fail = ch_dummy_file
 
             } else {
@@ -61,7 +61,7 @@ workflow ARRIBA_WORKFLOW {
                 ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
                 ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
-                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ meta, file -> return file }
+                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ it -> return it[1] }
             }
 
             if ( cram.contains('arriba') ) {
@@ -83,7 +83,7 @@ workflow ARRIBA_WORKFLOW {
 
             ch_arriba_fusions = reads
                 .combine(Channel.value( file(ch_dummy_file, checkIfExists: true ) ) )
-                .map { meta, reads, fusions -> [ meta, fusions ] }
+                .map { it -> [ it[0], it[2] ] }
 
             ch_arriba_fusion_fail = ch_dummy_file
         }
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 56fd8eb24..34102d2d2 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -118,10 +118,14 @@ workflow BUILD_REFERENCES {
         ch_arriba_ref_known_fusions = ARRIBA_DOWNLOAD.out.known_fusions
         ch_arriba_ref_protein_domains = ARRIBA_DOWNLOAD.out.protein_domains
     } else {
-        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist)
-        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands)
-        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions)
-        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
+        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { file ->
+        [ id: file.baseName, path: file ]}
+        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { file ->
+        [ id: file.baseName, path: file ]}
+        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { file ->
+        [ id: file.baseName, path: file ]}
+        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { file ->
+        [ id: file.baseName, path: file ]}
     }
 
 
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index f661aac6c..a60d6d137 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -4,27 +4,261 @@
             0,
             null,
             [
-                "pipeline_info"
+                
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-20T09:49:41.743056"
+        "timestamp": "2024-12-20T11:51:44.033131"
     },
     "stub test with fastp trim": {
         "content": [
-            0,
-            null,
+            18,
+            {
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.35
+                },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "fusioncatcher.py 1.35"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": null
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
-                "pipeline_info"
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
+                "fusioncatcher",
+                "fusioncatcher/human_v46",
+                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/fusion_report_db",
+                "references/fusion_report_db/DB-timestamp.txt",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/gffread",
+                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "star_for_arriba",
+                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
+                "star_for_arriba/test.Aligned.unsort.out.bam",
+                "star_for_arriba/test.Chimeric.out.junction",
+                "star_for_arriba/test.Log.final.out",
+                "star_for_arriba/test.Log.out",
+                "star_for_arriba/test.Log.progress.out",
+                "star_for_arriba/test.ReadsPerGene.out.tab",
+                "star_for_arriba/test.SJ.out.tab",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_arriba/test.out.sam",
+                "star_for_arriba/test.sortedByCoord.out.bam",
+                "star_for_arriba/test.tab",
+                "star_for_arriba/test.toTranscriptome.out.bam",
+                "star_for_arriba/test.unmapped_1.fastq.gz",
+                "star_for_arriba/test.unmapped_2.fastq.gz",
+                "star_for_arriba/testXd.out.bam",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.tab",
+                "star_for_starfusion/test.toTranscriptome.out.bam",
+                "star_for_starfusion/test.unmapped_1.fastq.gz",
+                "star_for_starfusion/test.unmapped_2.fastq.gz",
+                "star_for_starfusion/testXd.out.bam",
+                "starfusion",
+                "starfusion/ctat_genome_lib_build_dir",
+                "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-20T09:49:14.743338"
+        "timestamp": "2024-12-20T11:53:19.316547"
     }
 }
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index a755311c4..755be7b2e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -65,7 +65,7 @@ workflow RNAFUSION {
     //
     TRIM_WORKFLOW (
         ch_samplesheet,
-        params.adapter_fasta,
+        Channel.value(params.adapter_fasta),
         params.fastp_trim
     )
     ch_reads = TRIM_WORKFLOW.out.ch_reads_all

From 509a792e1197aeb69b22bb5218b6830cca9761cd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 20 Dec 2024 12:03:55 +0100
Subject: [PATCH 269/887] add species to schema

---
 nextflow_schema.json | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index db35f97da..1f83adc11 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -375,6 +375,11 @@
                     "type": "string",
                     "description": "Path to Fusion Annotation Library to be used in STARFUSION_BUILD.",
                     "fa_icon": "far fa-file-code"
+                },
+                "species": {
+                    "type": "string",
+                    "description": "Which species dfam should automatically download, default: human.",
+                    "fa_icon": "far fa-file-code"
                 }
             }
         },

From 8776835e747aaf65a0dbf5f493e5154b32cdbd11 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 20 Dec 2024 13:24:33 +0100
Subject: [PATCH 270/887] fix erroneous addition of meta

---
 subworkflows/local/arriba_workflow/main.nf |   8 +-
 subworkflows/local/build_references.nf     |  12 +-
 tests/test_stub.nf.test.snap               | 431 ++++++++++++++++++++-
 3 files changed, 432 insertions(+), 19 deletions(-)

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index bb4c36a68..5fa2ed2b3 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -52,10 +52,10 @@ workflow ARRIBA_WORKFLOW {
                     STAR_FOR_ARRIBA.out.bam,
                     ch_fasta,
                     ch_gtf,
-                    ch_arriba_ref_blacklist.map{ it[1] },
-                    ch_arriba_ref_known_fusions.map{ it[1] },
-                    ch_arriba_ref_cytobands.map{ it[1] },
-                    ch_arriba_ref_protein_domains.map{ it[1] }
+                    ch_arriba_ref_blacklist,
+                    ch_arriba_ref_known_fusions,
+                    ch_arriba_ref_cytobands,
+                    ch_arriba_ref_protein_domains
                 )
 
                 ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 34102d2d2..56fd8eb24 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -118,14 +118,10 @@ workflow BUILD_REFERENCES {
         ch_arriba_ref_known_fusions = ARRIBA_DOWNLOAD.out.known_fusions
         ch_arriba_ref_protein_domains = ARRIBA_DOWNLOAD.out.protein_domains
     } else {
-        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { file ->
-        [ id: file.baseName, path: file ]}
-        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { file ->
-        [ id: file.baseName, path: file ]}
-        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { file ->
-        [ id: file.baseName, path: file ]}
-        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { file ->
-        [ id: file.baseName, path: file ]}
+        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist)
+        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands)
+        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions)
+        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
     }
 
 
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index a60d6d137..e1892a6a9 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,22 +1,343 @@
 {
     "stub test no fastp trim": {
         "content": [
-            0,
-            null,
+            29,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.35
+                },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "fusioncatcher.py 1.35"
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": null
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
-                
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fusioncatcher",
+                "fusioncatcher/human_v46",
+                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusionreport",
+                "fusionreport/test",
+                "fusionreport/test/AAA_BBB.html",
+                "fusionreport/test/test.fusionreport.tsv",
+                "fusionreport/test/test.fusionreport_filtered.tsv",
+                "fusionreport/test/test.fusions.csv",
+                "fusionreport/test/test.fusions.json",
+                "fusionreport/test/test_fusionreport_index.html",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/fusion_report_db",
+                "references/fusion_report_db/DB-timestamp.txt",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/gffread",
+                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/salmon",
+                "references/salmon/salmon",
+                "references/salmon/salmon/complete_ref_lens.bin",
+                "references/salmon/salmon/ctable.bin",
+                "references/salmon/salmon/ctg_offsets.bin",
+                "references/salmon/salmon/duplicate_clusters.tsv",
+                "references/salmon/salmon/info.json",
+                "references/salmon/salmon/mphf.bin",
+                "references/salmon/salmon/pos.bin",
+                "references/salmon/salmon/pre_indexing.log",
+                "references/salmon/salmon/rank.bin",
+                "references/salmon/salmon/refAccumLengths.bin",
+                "references/salmon/salmon/ref_indexing.log",
+                "references/salmon/salmon/reflengths.bin",
+                "references/salmon/salmon/refseq.bin",
+                "references/salmon/salmon/seq.bin",
+                "references/salmon/salmon/versionInfo.json",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "star_for_arriba",
+                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
+                "star_for_arriba/test.Aligned.unsort.out.bam",
+                "star_for_arriba/test.Chimeric.out.junction",
+                "star_for_arriba/test.Log.final.out",
+                "star_for_arriba/test.Log.out",
+                "star_for_arriba/test.Log.progress.out",
+                "star_for_arriba/test.ReadsPerGene.out.tab",
+                "star_for_arriba/test.SJ.out.tab",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_arriba/test.out.sam",
+                "star_for_arriba/test.sortedByCoord.out.bam",
+                "star_for_arriba/test.tab",
+                "star_for_arriba/test.toTranscriptome.out.bam",
+                "star_for_arriba/test.unmapped_1.fastq.gz",
+                "star_for_arriba/test.unmapped_2.fastq.gz",
+                "star_for_arriba/testXd.out.bam",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
+                "star_for_starfusion/test.tab",
+                "star_for_starfusion/test.toTranscriptome.out.bam",
+                "star_for_starfusion/test.unmapped_1.fastq.gz",
+                "star_for_starfusion/test.unmapped_2.fastq.gz",
+                "star_for_starfusion/testXd.out.bam",
+                "starfusion",
+                "starfusion/ctat_genome_lib_build_dir",
+                "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-20T11:51:44.033131"
+        "timestamp": "2024-12-20T13:17:20.018293"
     },
     "stub test with fastp trim": {
         "content": [
-            18,
+            31,
             {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
@@ -35,12 +356,22 @@
                 "FUSIONCATCHER_BUILD": {
                     "fusioncatcher": "fusioncatcher.py 1.35"
                 },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "2.1.5"
                 },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
                 "GENCODE_DOWNLOAD": {
                     "wget": null
                 },
@@ -56,9 +387,27 @@
                 "HGNC_DOWNLOAD": {
                     "wget": null
                 },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
                 "STARFUSION_BUILD": {
                     "STAR-Fusion": "1.14.0"
                 },
@@ -67,16 +416,30 @@
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
+                "STAR_FOR_STARFUSION": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
                 "STAR_GENOMEGENERATE": {
                     "star": null,
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -95,11 +458,32 @@
                 "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
                 "fusioncatcher/test.fusioncatcher.log",
                 "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusionreport",
+                "fusionreport/test",
+                "fusionreport/test/AAA_BBB.html",
+                "fusionreport/test/test.fusionreport.tsv",
+                "fusionreport/test/test.fusionreport_filtered.tsv",
+                "fusionreport/test/test.fusions.csv",
+                "fusionreport/test/test.fusions.json",
+                "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "get",
                 "get/rrna.bed",
                 "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
                 "references",
@@ -116,6 +500,7 @@
                 "references/fusion_report_db/mitelman.db",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
@@ -124,6 +509,23 @@
                 "references/hgnc",
                 "references/hgnc/HGNC-DB-timestamp.txt",
                 "references/hgnc/hgnc_complete_set.txt",
+                "references/salmon",
+                "references/salmon/salmon",
+                "references/salmon/salmon/complete_ref_lens.bin",
+                "references/salmon/salmon/ctable.bin",
+                "references/salmon/salmon/ctg_offsets.bin",
+                "references/salmon/salmon/duplicate_clusters.tsv",
+                "references/salmon/salmon/info.json",
+                "references/salmon/salmon/mphf.bin",
+                "references/salmon/salmon/pos.bin",
+                "references/salmon/salmon/pre_indexing.log",
+                "references/salmon/salmon/rank.bin",
+                "references/salmon/salmon/refAccumLengths.bin",
+                "references/salmon/salmon/ref_indexing.log",
+                "references/salmon/salmon/reflengths.bin",
+                "references/salmon/salmon/refseq.bin",
+                "references/salmon/salmon/seq.bin",
+                "references/salmon/salmon/versionInfo.json",
                 "references/star",
                 "references/star/Genome",
                 "references/star/Log.out",
@@ -141,6 +543,10 @@
                 "references/star/sjdbList.fromGTF.out.tab",
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
                 "star_for_arriba",
                 "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
                 "star_for_arriba/test.Aligned.unsort.out.bam",
@@ -172,6 +578,7 @@
                 "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
                 "star_for_starfusion/test.out.sam",
                 "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
                 "star_for_starfusion/test.tab",
                 "star_for_starfusion/test.toTranscriptome.out.bam",
                 "star_for_starfusion/test.unmapped_1.fastq.gz",
@@ -252,13 +659,23 @@
                 "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
                 "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
                 "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz"
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-20T11:53:19.316547"
+        "timestamp": "2024-12-20T13:15:06.974011"
     }
 }
\ No newline at end of file

From ab65540133a556fda772f776a3bc4391a1f12329 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 20 Dec 2024 14:02:55 +0100
Subject: [PATCH 271/887] fixes

---
 tests/test_stub.nf.test.snap | 78 +++++++++++++++++++++++++++++++-----
 workflows/rnafusion.nf       |  6 +--
 2 files changed, 70 insertions(+), 14 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index e1892a6a9..0ac620e73 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,7 +1,7 @@
 {
     "stub test no fastp trim": {
         "content": [
-            29,
+            31,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -9,6 +9,9 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "CTATSPLICING_STARTOCANCERINTRONS": {
+                    "ctat-splicing": "0.0.2"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -74,17 +77,17 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -102,6 +105,31 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "ctatsplicing",
+                "ctatsplicing/arriba",
+                "ctatsplicing/arriba/test.cancer.introns",
+                "ctatsplicing/arriba/test.cancer.introns.prelim",
+                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/arriba/test.chckpts",
+                "ctatsplicing/arriba/test.ctat-splicing.igv.html",
+                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/arriba/test.igv.tracks",
+                "ctatsplicing/arriba/test.introns",
+                "ctatsplicing/arriba/test.introns.for_IGV.bed",
+                "ctatsplicing/starfusion",
+                "ctatsplicing/starfusion/test.cancer.introns",
+                "ctatsplicing/starfusion/test.cancer.introns.prelim",
+                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/starfusion/test.chckpts",
+                "ctatsplicing/starfusion/test.ctat-splicing.igv.html",
+                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/starfusion/test.igv.tracks",
+                "ctatsplicing/starfusion/test.introns",
+                "ctatsplicing/starfusion/test.introns.for_IGV.bed",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -329,11 +357,11 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-20T13:17:20.018293"
+        "timestamp": "2024-12-20T14:00:19.311265"
     },
     "stub test with fastp trim": {
         "content": [
-            31,
+            33,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -341,6 +369,9 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "CTATSPLICING_STARTOCANCERINTRONS": {
+                    "ctat-splicing": "0.0.2"
+                },
                 "FASTP": {
                     "fastp": "0.23.4"
                 },
@@ -412,17 +443,17 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -440,6 +471,31 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "ctatsplicing",
+                "ctatsplicing/arriba",
+                "ctatsplicing/arriba/test.cancer.introns",
+                "ctatsplicing/arriba/test.cancer.introns.prelim",
+                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/arriba/test.chckpts",
+                "ctatsplicing/arriba/test.ctat-splicing.igv.html",
+                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/arriba/test.igv.tracks",
+                "ctatsplicing/arriba/test.introns",
+                "ctatsplicing/arriba/test.introns.for_IGV.bed",
+                "ctatsplicing/starfusion",
+                "ctatsplicing/starfusion/test.cancer.introns",
+                "ctatsplicing/starfusion/test.cancer.introns.prelim",
+                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/starfusion/test.chckpts",
+                "ctatsplicing/starfusion/test.ctat-splicing.igv.html",
+                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/starfusion/test.igv.tracks",
+                "ctatsplicing/starfusion/test.introns",
+                "ctatsplicing/starfusion/test.introns.for_IGV.bed",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -676,6 +732,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-20T13:15:06.974011"
+        "timestamp": "2024-12-20T13:57:10.179877"
     }
-}
\ No newline at end of file
+}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 3c98cf6b3..857827aa5 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -92,7 +92,7 @@ workflow RNAFUSION {
         BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
         BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
         BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
-        ch_starfusion_ref,
+        BUILD_REFERENCES.out.ch_starfusion_ref,
         params.arriba,                   // boolean
         params.all,                      // boolean
         params.fusioninspector_only,     // boolean
@@ -110,8 +110,8 @@ workflow RNAFUSION {
         ch_reads,
         BUILD_REFERENCES.out.ch_gtf,
         BUILD_REFERENCES.out.ch_starindex_ref,
-        BUILD_REFERENCES.out.ch_fasta
-        ch_starfusion_ref
+        BUILD_REFERENCES.out.ch_fasta,
+        BUILD_REFERENCES.out.ch_starfusion_ref
     )
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
 

From 7c333f51321d4193c4c7953af5e057e045ec9443 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Fri, 20 Dec 2024 13:11:47 +0000
Subject: [PATCH 272/887] Template update for nf-core/tools version 3.1.1

---
 .editorconfig                                 |  4 +
 .github/ISSUE_TEMPLATE/bug_report.yml         |  1 -
 .github/workflows/download_pipeline.yml       | 41 ++++++----
 .nf-core.yml                                  |  2 +-
 .prettierignore                               |  1 +
 CITATIONS.md                                  |  4 +-
 LICENSE                                       |  2 +-
 README.md                                     | 15 +---
 docs/output.md                                | 11 +--
 docs/usage.md                                 |  2 +-
 nextflow.config                               |  3 +
 ro-crate-metadata.json                        | 81 ++++++++++++-------
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  2 +-
 13 files changed, 96 insertions(+), 73 deletions(-)

diff --git a/.editorconfig b/.editorconfig
index 72dda289a..6d9b74cc0 100644
--- a/.editorconfig
+++ b/.editorconfig
@@ -31,3 +31,7 @@ indent_size = unset
 # ignore python and markdown
 [*.{py,md}]
 indent_style = unset
+
+# ignore ro-crate metadata files
+[**/ro-crate-metadata.json]
+insert_final_newline = unset
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index 7755ffb4a..5980dff43 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -9,7 +9,6 @@ body:
 
         - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
         - [nf-core/rnafusion pipeline documentation](https://nf-co.re/rnafusion/usage)
-
   - type: textarea
     id: description
     attributes:
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 2576cc0c7..13b51e2c3 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -28,8 +28,12 @@ env:
   NXF_ANSI_LOG: false
 
 jobs:
-  download:
+  configure:
     runs-on: ubuntu-latest
+    outputs:
+      REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }}
+      REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }}
+      REPO_BRANCH: ${{ steps.get_repo_properties.outputs.REPO_BRANCH }}
     steps:
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
@@ -53,22 +57,27 @@ jobs:
           pip install git+https://github.com/nf-core/tools.git@dev
 
       - name: Get the repository name and current branch set as environment variable
+        id: get_repo_properties
         run: |
-          echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV}
-          echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
-          echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV}
+          echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT"
+          echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT"
+          echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT"
 
       - name: Make a cache directory for the container images
         run: |
           mkdir -p ./singularity_container_images
 
+  download:
+    runs-on: ubuntu-latest
+    needs: configure
+    steps:
       - name: Download the pipeline
         env:
           NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
         run: |
-          nf-core pipelines download ${{ env.REPO_LOWERCASE }} \
-          --revision ${{ env.REPO_BRANCH }} \
-          --outdir ./${{ env.REPOTITLE_LOWERCASE }} \
+          nf-core pipelines download ${{ needs.configure.outputs.REPO_LOWERCASE }} \
+          --revision ${{ needs.configure.outputs.REPO_BRANCH }} \
+          --outdir ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} \
           --compress "none" \
           --container-system 'singularity' \
           --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io/library/" \
@@ -76,14 +85,14 @@ jobs:
           --download-configuration 'yes'
 
       - name: Inspect download
-        run: tree ./${{ env.REPOTITLE_LOWERCASE }}
+        run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}
 
       - name: Count the downloaded number of container images
         id: count_initial
         run: |
           image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
           echo "Initial container image count: $image_count"
-          echo "IMAGE_COUNT_INITIAL=$image_count" >> ${GITHUB_ENV}
+          echo "IMAGE_COUNT_INITIAL=$image_count" >> "$GITHUB_OUTPUT"
 
       - name: Run the downloaded pipeline (stub)
         id: stub_run_pipeline
@@ -91,27 +100,27 @@ jobs:
         env:
           NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
           NXF_SINGULARITY_HOME_MOUNT: true
-        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
+        run: nextflow run ./${{needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
       - name: Run the downloaded pipeline (stub run not supported)
         id: run_pipeline
-        if: ${{ job.steps.stub_run_pipeline.status == failure() }}
+        if: ${{ steps.stub_run_pipeline.outcome == 'failure' }}
         env:
           NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
           NXF_SINGULARITY_HOME_MOUNT: true
-        run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
+        run: nextflow run ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -profile test,singularity --outdir ./results
 
       - name: Count the downloaded number of container images
         id: count_afterwards
         run: |
           image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
           echo "Post-pipeline run container image count: $image_count"
-          echo "IMAGE_COUNT_AFTER=$image_count" >> ${GITHUB_ENV}
+          echo "IMAGE_COUNT_AFTER=$image_count" >> "$GITHUB_OUTPUT"
 
       - name: Compare container image counts
         run: |
-          if [ "${{ env.IMAGE_COUNT_INITIAL }}" -ne "${{ env.IMAGE_COUNT_AFTER }}" ]; then
-            initial_count=${{ env.IMAGE_COUNT_INITIAL }}
-            final_count=${{ env.IMAGE_COUNT_AFTER }}
+          if [ "${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}" -ne "${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}" ]; then
+            initial_count=${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}
+            final_count=${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}
             difference=$((final_count - initial_count))
             echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!"
             tree ./singularity_container_images
diff --git a/.nf-core.yml b/.nf-core.yml
index 37d9e4cd7..148865a49 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,7 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
-nf_core_version: 3.1.0
+nf_core_version: 3.1.1
 repository_type: pipeline
 template:
   author: Martin Proks, Annick Renevey
diff --git a/.prettierignore b/.prettierignore
index 437d763d0..edd29f01e 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -10,3 +10,4 @@ testing/
 testing*
 *.pyc
 bin/
+ro-crate-metadata.json
diff --git a/CITATIONS.md b/CITATIONS.md
index 0fc81801b..93ddd1a75 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -12,9 +12,7 @@
 
 - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
 
-> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].
-
-- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
+> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
 
 > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
diff --git a/LICENSE b/LICENSE
index 86e71fe15..feb286272 100644
--- a/LICENSE
+++ b/LICENSE
@@ -1,6 +1,6 @@
 MIT License
 
-Copyright (c) Martin Proks, Annick Renevey
+Copyright (c) The nf-core/rnafusion team
 
 Permission is hereby granted, free of charge, to any person obtaining a copy
 of this software and associated documentation files (the "Software"), to deal
diff --git a/README.md b/README.md
index 932243be4..12b71f87f 100644
--- a/README.md
+++ b/README.md
@@ -3,9 +3,7 @@
     <source media="(prefers-color-scheme: dark)" srcset="docs/images/nf-core-rnafusion_logo_dark.png">
     <img alt="nf-core/rnafusion" src="docs/images/nf-core-rnafusion_logo_light.png">
   </picture>
-</h1>
-
-[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)
+</h1>[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
@@ -29,15 +27,12 @@
 
 <!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
      workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->
-<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->
-
-1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 
 ## Usage
 
 > [!NOTE]
-> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
+> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
 
 <!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.
      Explain what rows and columns represent. For instance (please edit as appropriate):
@@ -94,9 +89,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 ## Citations
 
 <!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
-<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
-
-<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
+<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) --><!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
 
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
diff --git a/docs/output.md b/docs/output.md
index 501e19501..f5beb7911 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -12,8 +12,7 @@ The directories listed below will be created in the results directory after the
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
-- [FastQC](#fastqc) - Raw read QC
-- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+- [FastQC](#fastqc) - Raw read QC- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
 - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### FastQC
@@ -27,9 +26,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 </details>
 
-[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
-
-### MultiQC
+[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).### MultiQC
 
 <details markdown="1">
 <summary>Output files</summary>
@@ -43,9 +40,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
 
-Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
-
-### Pipeline information
+Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.### Pipeline information
 
 <details markdown="1">
 <summary>Output files</summary>
diff --git a/docs/usage.md b/docs/usage.md
index c8a121d09..73894cb12 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -130,7 +130,7 @@ Several generic profiles are bundled with the pipeline which instruct the pipeli
 > [!IMPORTANT]
 > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
 
-The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is suported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
+The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is supported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
 
 Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important!
 They are loaded in sequence, so later profiles can overwrite earlier profiles.
diff --git a/nextflow.config b/nextflow.config
index 4383d5421..bf863573a 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -291,3 +291,6 @@ validation {
         afterText = validation.help.afterText
     }
 }
+
+// Load modules.config for DSL2 module specific options
+includeConfig 'conf/modules.config'
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index f1d7090ab..145ad4d00 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,8 +22,8 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
-            "datePublished": "2024-12-12T11:24:21+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "datePublished": "2024-12-20T13:11:36+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) --><!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#e018c319-519d-4a99-bdba-7c9c8860b896"
+                    "@id": "#f907a90b-3444-4392-bd9f-cad7780c2dc3"
                 }
             ],
             "name": "nf-core/rnafusion"
@@ -121,40 +121,61 @@
         },
         {
             "@id": "main.nf",
-            "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
+            "@type": [
+                "File",
+                "SoftwareSourceCode",
+                "ComputationalWorkflow"
+            ],
             "creator": [
                 {
-                    "@id": "https://orcid.org/0000-0001-9017-591X"
+                    "@id": "https://orcid.org/0000-0002-8178-3128"
                 },
                 {
-                    "@id": "#phil.ewels@scilifelab.se"
+                    "@id": "#max.u.garcia@gmail.com"
                 },
                 {
-                    "@id": "https://orcid.org/0000-0002-8178-3128"
+                    "@id": "#phil.ewels@scilifelab.se"
                 },
                 {
-                    "@id": "#max.u.garcia@gmail.com"
+                    "@id": "https://orcid.org/0000-0001-9017-591X"
                 }
             ],
             "dateCreated": "",
-            "dateModified": "2024-12-12T11:24:21Z",
+            "dateModified": "2024-12-20T13:11:36Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
-            "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
-            "license": ["MIT"],
+            "keywords": [
+                "nf-core",
+                "nextflow",
+                "fusion",
+                "fusion-genes",
+                "gene-fusion",
+                "rna",
+                "rna-seq"
+            ],
+            "license": [
+                "MIT"
+            ],
             "maintainer": [
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
                 }
             ],
-            "name": ["nf-core/rnafusion"],
+            "name": [
+                "nf-core/rnafusion"
+            ],
             "programmingLanguage": {
                 "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow"
             },
             "sdPublisher": {
                 "@id": "https://nf-co.re/"
             },
-            "url": ["https://github.com/nf-core/rnafusion", "https://nf-co.re/rnafusion/dev/"],
-            "version": ["4.0.0dev"]
+            "url": [
+                "https://github.com/nf-core/rnafusion",
+                "https://nf-co.re/rnafusion/dev/"
+            ],
+            "version": [
+                "4.0.0dev"
+            ]
         },
         {
             "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow",
@@ -169,11 +190,11 @@
             "version": "!>=24.04.2"
         },
         {
-            "@id": "#e018c319-519d-4a99-bdba-7c9c8860b896",
+            "@id": "#f907a90b-3444-4392-bd9f-cad7780c2dc3",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#36a96043-5ef5-4bc3-86eb-32712383dda8"
+                    "@id": "#7a00a302-505f-4b06-bd1c-5a4e1fa19bdd"
                 }
             ],
             "mainEntity": {
@@ -182,7 +203,7 @@
             "name": "Test suite for nf-core/rnafusion"
         },
         {
-            "@id": "#36a96043-5ef5-4bc3-86eb-32712383dda8",
+            "@id": "#7a00a302-505f-4b06-bd1c-5a4e1fa19bdd",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/rnafusion",
             "resource": "repos/nf-core/rnafusion/actions/workflows/ci.yml",
@@ -310,18 +331,6 @@
             "name": "nf-core",
             "url": "https://nf-co.re/"
         },
-        {
-            "@id": "https://orcid.org/0000-0001-9017-591X",
-            "@type": "Person",
-            "email": "rickard.hammaren@scilifelab.se",
-            "name": "Rickard Hammar\u00e9n"
-        },
-        {
-            "@id": "#phil.ewels@scilifelab.se",
-            "@type": "Person",
-            "email": "phil.ewels@scilifelab.se",
-            "name": "Phil Ewels"
-        },
         {
             "@id": "https://orcid.org/0000-0002-8178-3128",
             "@type": "Person",
@@ -333,6 +342,18 @@
             "@type": "Person",
             "email": "max.u.garcia@gmail.com",
             "name": "Maxime Garcia"
+        },
+        {
+            "@id": "#phil.ewels@scilifelab.se",
+            "@type": "Person",
+            "email": "phil.ewels@scilifelab.se",
+            "name": "Phil Ewels"
+        },
+        {
+            "@id": "https://orcid.org/0000-0001-9017-591X",
+            "@type": "Person",
+            "email": "rickard.hammaren@scilifelab.se",
+            "name": "Rickard Hammar\u00e9n"
         }
     ]
-}
+}
\ No newline at end of file
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index df68ef3ab..9ec640a61 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -117,7 +117,7 @@ workflow PIPELINE_COMPLETION {
     main:
     summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
     def multiqc_reports = multiqc_report.toList()
-    
+
     //
     // Completion email and summary
     //

From ed4855edee9e3c01b3b8c8bb12c120c5676ebc82 Mon Sep 17 00:00:00 2001
From: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 20 Dec 2024 11:07:14 -0300
Subject: [PATCH 273/887] Update modules/local/starfusion/detect/main.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 modules/local/starfusion/detect/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index fd0a7d46b..f1f3d6f94 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -19,7 +19,7 @@ process STARFUSION {
 
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def fasta = reads ? (meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}") : ""
+    def fastq_arg = reads ? (meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}") : ""
     def junction_arg =  junction ? "-J ${junction}" : ""
     def args = task.ext.args ?: ''
     """

From f42d9dcf8917dc4c5ab982bc64b58d44ebff2298 Mon Sep 17 00:00:00 2001
From: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 20 Dec 2024 11:07:21 -0300
Subject: [PATCH 274/887] Update modules/local/starfusion/detect/main.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 modules/local/starfusion/detect/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index f1f3d6f94..00c78efc7 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -25,7 +25,7 @@ process STARFUSION {
     """
     STAR-Fusion \\
         --genome_lib_dir $reference \\
-        $fasta \\
+        $fastq_arg \\
         $junction_arg \\
         --CPU $task.cpus \\
         --examine_coding_effect \\

From 910cf8d755973f0eb57bd0d1be4d69c23dda1597 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 23 Dec 2024 18:27:28 +0000
Subject: [PATCH 275/887] test: add nf-test for starfusion subworkflow

---
 .../main.nf}                                  |  15 +--
 .../starfusion_workflow/tests/main.nf.test    | 115 ++++++++++++++++++
 .../tests/main.nf.test.snap                   |  38 ++++++
 .../starfusion_workflow/tests/nextflow.config |  37 ++++++
 4 files changed, 198 insertions(+), 7 deletions(-)
 rename subworkflows/local/{starfusion_workflow.nf => starfusion_workflow/main.nf} (83%)
 create mode 100644 subworkflows/local/starfusion_workflow/tests/main.nf.test
 create mode 100644 subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
 create mode 100644 subworkflows/local/starfusion_workflow/tests/nextflow.config

diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow/main.nf
similarity index 83%
rename from subworkflows/local/starfusion_workflow.nf
rename to subworkflows/local/starfusion_workflow/main.nf
index 7f12f08a5..ed8d011ed 100644
--- a/subworkflows/local/starfusion_workflow.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -1,10 +1,9 @@
-include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION }      from '../../modules/nf-core/samtools/index/main'
-include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION_CRAM } from '../../modules/nf-core/samtools/index/main'
-include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_STARFUSION }        from '../../modules/nf-core/samtools/view/main'
-include { STAR_ALIGN as STAR_FOR_STARFUSION }                    from '../../modules/nf-core/star/align/main'
-include { STARFUSION }                                           from '../../modules/local/starfusion/detect/main'
-
-include { CTATSPLICING_WORKFLOW         }   from './ctatsplicing_workflow'
+include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION       }   from '../../../modules/nf-core/samtools/index/main'
+include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION_CRAM  }   from '../../../modules/nf-core/samtools/index/main'
+include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_STARFUSION         }   from '../../../modules/nf-core/samtools/view/main'
+include { STAR_ALIGN as STAR_FOR_STARFUSION                     }   from '../../../modules/nf-core/star/align/main'
+include { STARFUSION                                            }   from '../../../modules/local/starfusion/detect/main'
+include { CTATSPLICING_WORKFLOW                                 }   from '../ctatsplicing_workflow'
 
 workflow STARFUSION_WORKFLOW {
     take:
@@ -31,6 +30,8 @@ workflow STARFUSION_WORKFLOW {
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
                 ch_align = STAR_FOR_STARFUSION.out.bam_sorted
 
+                ch_align.view()
+
                 SAMTOOLS_INDEX_FOR_STARFUSION(STAR_FOR_STARFUSION.out.bam_sorted)
                 ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION.out.versions)
                 bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai)
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
new file mode 100644
index 000000000..ac0e77982
--- /dev/null
+++ b/subworkflows/local/starfusion_workflow/tests/main.nf.test
@@ -0,0 +1,115 @@
+nextflow_workflow {
+
+    name "Test Subworkflow STARFUSION_WORKFLOW"
+    script "../main.nf"
+    workflow "STARFUSION_WORKFLOW"
+    tag "subworkflow"
+    tag "star"
+    tag "star/genomegenerate"
+    tag "star/align"
+
+
+    test("STARFUSION_WORKFLOW - Homo sapiens - FASTQs minigenome") {
+        config './nextflow.config'
+
+        setup {
+            run("STAR_GENOMEGENERATE") {
+                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
+                process {
+                """
+                    // FASTA
+                    input[0] = Channel.fromPath(
+                            "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+
+                    // GTF
+                    input[1] = Channel.fromPath(
+                            "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf", checkIfExists: true
+                        )
+                        .map{ [[id: it.getName() ], it ]}
+                    """
+                }
+            }
+
+            run("STARFUSION_BUILD") {
+            script "../../../../modules/local/starfusion/build/main.nf"
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                ]
+                input[1] = [
+                    [ id:'minigenome gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                ]
+
+                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input [3] = "human"
+                """
+            }
+        }
+        }
+
+        when {
+            workflow {
+                """
+                // ch_reads
+                input[0] = Channel.of(
+                    [
+                        [ id: "test_fastqs" ],
+                        [
+                            file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz", checkIfExists: true),
+                            file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz", checkIfExists: true)
+                        ]
+                    ] )
+
+                // ch_gtf
+                input[1] =
+                    Channel.fromPath(
+                        "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_starindex_ref
+                input[2] = STAR_GENOMEGENERATE.out.index
+
+                // ch_fasta
+                input[3] =
+                    Channel.fromPath(
+                        "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa", checkIfExists: true
+                    )
+                    .map{ [ [ id: it.name ], it ] }
+
+                // ch_starfusion_ref
+                input[4] = STARFUSION_BUILD.out.reference
+
+                """
+            }
+            params {
+                starfusion = true
+                cram = 'starfusion'
+                outdir = "$outputDir"
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(
+                    workflow.out.fusions,
+                    file(workflow.out.star_stats[0][1]).name,
+                    workflow.out.star_gene_count,
+                    workflow.out.ch_bam_sorted,
+                    workflow.out.ch_bam_sorted_indexed,
+                    workflow.out.versions
+                ).match() }
+            )
+        }
+    }
+
+
+
+
+}
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap b/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..5bc897e7f
--- /dev/null
+++ b/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
@@ -0,0 +1,38 @@
+{
+    "STARFUSION_WORKFLOW - Homo sapiens - FASTQs minigenome": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test_fastqs"
+                    },
+                    "test_fastqs.starfusion.fusion_predictions.tsv:md5,abe17134a231642edf9351e4964e8a97"
+                ]
+            ],
+            "test_fastqs.Log.final.out",
+            [
+                [
+                    {
+                        "id": "test_fastqs"
+                    },
+                    "test_fastqs.ReadsPerGene.out.tab:md5,8e0d42deeea09924d5c7ba3147bbfd78"
+                ]
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,afde4f2fd6056df81e322b3c35ab7a8a",
+                "versions.yml:md5,e53f1ec32bc78a33f99892e42274833a"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2024-12-23T18:25:52.838411738"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/local/starfusion_workflow/tests/nextflow.config b/subworkflows/local/starfusion_workflow/tests/nextflow.config
new file mode 100644
index 000000000..718add69c
--- /dev/null
+++ b/subworkflows/local/starfusion_workflow/tests/nextflow.config
@@ -0,0 +1,37 @@
+process {
+    withName: 'STAR_FOR_STARFUSION' {
+        ext.args = '--twopassMode Basic \
+        --outReadsUnmapped None \
+        --readFilesCommand zcat \
+        --outSAMtype BAM SortedByCoordinate \
+        --outSAMstrandField intronMotif \
+        --outSAMunmapped Within \
+        --chimSegmentMin 12 \
+        --chimJunctionOverhangMin 8 \
+        --chimOutJunctionFormat 1 \
+        --alignSJDBoverhangMin 10 \
+        --alignMatesGapMax 100000 \
+        --alignIntronMax 100000 \
+        --alignSJstitchMismatchNmax 5 -1 5 5 \
+        --chimMultimapScoreRange 3 \
+        --chimScoreJunctionNonGTAG -4 \
+        --chimMultimapNmax 20 \
+        --chimNonchimScoreDropMin 10 \
+        --peOverlapNbasesMin 12 \
+        --peOverlapMMp 0.1 \
+        --alignInsertionFlush Right \
+        --alignSplicedMateMapLminOverLmate 0 \
+        --alignSplicedMateMapLmin 30 \
+        --chimOutType Junctions \
+        --quantMode GeneCounts'
+    }
+
+        withName: 'SAMTOOLS_INDEX_FOR_STARFUSION_CRAM' {
+        ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
+    }
+
+    withName: 'SAMTOOLS_VIEW_FOR_STARFUSION' {
+        ext.args = { "--output-fmt cram" }
+        ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
+    }
+}

From e23ff3919dc86d6f33a580c01c0212df77ce1810 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 23 Dec 2024 18:29:18 +0000
Subject: [PATCH 276/887] chore: add todo message

---
 subworkflows/local/starfusion_workflow/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index ed8d011ed..11aa816b9 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -28,7 +28,7 @@ workflow STARFUSION_WORKFLOW {
             } else {
                 STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
-                ch_align = STAR_FOR_STARFUSION.out.bam_sorted
+                ch_align = STAR_FOR_STARFUSION.out.bam_sorted // TODO: This does not seem to be captured and used as the output is bam_sorted_aligned and not bam_sorted
 
                 ch_align.view()
 

From d5ce9dca56b51ad5a10a4d32bcf052773d041958 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 23 Dec 2024 18:31:18 +0000
Subject: [PATCH 277/887] fix: remove commited debug view

---
 subworkflows/local/starfusion_workflow/main.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index 11aa816b9..938832c96 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -30,7 +30,6 @@ workflow STARFUSION_WORKFLOW {
                 ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
                 ch_align = STAR_FOR_STARFUSION.out.bam_sorted // TODO: This does not seem to be captured and used as the output is bam_sorted_aligned and not bam_sorted
 
-                ch_align.view()
 
                 SAMTOOLS_INDEX_FOR_STARFUSION(STAR_FOR_STARFUSION.out.bam_sorted)
                 ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION.out.versions)

From ef8897794ac01fb7d24263669ae5680bf01940e7 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 23 Dec 2024 18:34:16 +0000
Subject: [PATCH 278/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d947b9004..e7288298d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `STARFUSION_DETECT`. [#586](https://github.com/nf-core/rnafusion/pull/586)
 - Added a new module `CTATSPLICING_STARTOCANCERINTRONS` and a new parameter `--ctatsplicing`. This options creates reports on cancer splicing abberations and requires one or both of `--arriba` and `--starfusion` to be given. [#587](https://github.com/nf-core/rnafusion/pull/587)
 - Add parameter `--references_only` when no data should be analysed, but only the references should be built [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)
 
 ### Changed
 

From 046620eeaa50507047294f14ac5b405d6681d994 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 23 Dec 2024 18:52:18 +0000
Subject: [PATCH 279/887] chore: remove unused module
 `starfusion_downloadgenome`

---
 conf/modules.config                           | 10 ------
 .../local/starfusion/download/environment.yml |  8 -----
 modules/local/starfusion/download/main.nf     | 34 -------------------
 modules/local/starfusion/download/meta.yml    | 25 --------------
 4 files changed, 77 deletions(-)
 delete mode 100644 modules/local/starfusion/download/environment.yml
 delete mode 100644 modules/local/starfusion/download/main.nf
 delete mode 100644 modules/local/starfusion/download/meta.yml

diff --git a/conf/modules.config b/conf/modules.config
index b3643f48f..31a1d208f 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -371,16 +371,6 @@ process {
         ext.args = "--max_readlength ${params.read_length} --human_gencode_filter"
     }
 
-    withName: 'STARFUSION_DOWNLOAD' {
-        cpus   = { 2    * task.attempt }
-        memory = { 24.GB * task.attempt }
-        time   = { 6.h  * task.attempt }
-        publishDir = [
-            path: { "${params.genomes_base}/starfusion" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-        ]
-    }
 
     withName: 'STRINGTIE_MERGE' {
         publishDir = [
diff --git a/modules/local/starfusion/download/environment.yml b/modules/local/starfusion/download/environment.yml
deleted file mode 100644
index ef7f93160..000000000
--- a/modules/local/starfusion/download/environment.yml
+++ /dev/null
@@ -1,8 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::dfam=3.7
-  - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.28
-  - bioconda::star-fusion=1.14.0
diff --git a/modules/local/starfusion/download/main.nf b/modules/local/starfusion/download/main.nf
deleted file mode 100644
index ace18a9e7..000000000
--- a/modules/local/starfusion/download/main.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-process STARFUSION_DOWNLOAD {
-    tag 'star-fusion'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
-
-    output:
-    path "ctat_genome_lib_build_dir/*"            , emit: reference
-
-
-    // TODO: move to S3
-    script:
-    """
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/__genome_libs_StarFv1.10/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz --no-check-certificate
-
-    tar xvf GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
-
-    rm GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play.tar.gz
-
-    mv */ctat_genome_lib_build_dir .
-    """
-
-    stub:
-    """
-    mkdir ctat_genome_lib_build_dir
-    touch ref_annot.cdna.fa
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/starfusion/download/meta.yml b/modules/local/starfusion/download/meta.yml
deleted file mode 100644
index 24e842520..000000000
--- a/modules/local/starfusion/download/meta.yml
+++ /dev/null
@@ -1,25 +0,0 @@
-name: starfusion_downloadgenome
-description: Download STAR-fusion genome resource required to run STAR-Fusion caller
-keywords:
-  - downoad
-tools:
-  - star-fusion:
-      description: Fusion calling algorithm for RNAseq data
-      homepage: https://github.com/STAR-Fusion/
-      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
-      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
-      doi: "10.1186/s13059-019-1842-9"
-      licence: ["GPL v3"]
-
-output:
-  - reference:
-      type: directory
-      description: Genome resource path
-      pattern: "star-fusion-genome"
-  - gtf:
-      type: file
-      description: genome gtf file
-      pattern: "*.{gtf}"
-
-authors:
-  - "@praveenraj2018,@rannick"

From 3d82388333e3ac40c9e37c1821026366478828e3 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 23 Dec 2024 18:52:36 +0000
Subject: [PATCH 280/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d947b9004..32c7dca90 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -39,6 +39,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Integrate reference building in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Move from ensembl to gencode base [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Update from ensembl 102 to gencode 46 default references [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Remove local module `STARFUSION_DOWNLOAD` [#598](https://github.com/nf-core/rnafusion/pull/598)
 
 ### Fixed
 

From b514ff6742396efdbc99a32ef59d95651df31c2e Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 26 Dec 2024 14:33:16 +0000
Subject: [PATCH 281/887] feat: update fusioinspector to 2.10.0

---
 modules/local/fusioninspector/environment.yml | 5 +----
 modules/local/fusioninspector/main.nf         | 4 ++--
 2 files changed, 3 insertions(+), 6 deletions(-)

diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index ef7f93160..ce6545e4d 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -2,7 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::dfam=3.7
-  - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.28
-  - bioconda::star-fusion=1.14.0
+  - bioconda::fusion-inspector=2.10.0
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index ade7c174a..c7f613b47 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d98081910b41caa0b62d892e7a44543183111afa2b0a5eea5a4a9472eea72611/data' :
+        'community.wave.seqera.io/library/fusion-inspector:2.10.0--b59230553c98db81'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)

From 96485cce0088c02de624d027673e090eddc22451 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 26 Dec 2024 18:19:53 +0000
Subject: [PATCH 282/887] fix: update container with missing dependencies

---
 modules/local/fusioninspector/environment.yml | 6 ++++++
 modules/local/fusioninspector/main.nf         | 4 ++--
 2 files changed, 8 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index ce6545e4d..821d154d5 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -3,3 +3,9 @@ channels:
   - bioconda
 dependencies:
   - bioconda::fusion-inspector=2.10.0
+  - bioconda::igv-reports=1.14.1
+  - bioconda::perl-json-xs=4.03
+  - bioconda::pysam=0.22.1
+  - pip
+  - pip:
+      - intervaltree==3.1.0
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index c7f613b47..9ec2c3db9 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d98081910b41caa0b62d892e7a44543183111afa2b0a5eea5a4a9472eea72611/data' :
-        'community.wave.seqera.io/library/fusion-inspector:2.10.0--b59230553c98db81'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0eb08b2bd07e8012eabea34ec26304ef36b3a3bf338cdd6658ce7ab8a9c2937/data' :
+        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:d78d0c462ca32766'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)

From 10c6b563eef982ee6acaa3e4527288d92f0e33f0 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 26 Dec 2024 18:39:45 +0000
Subject: [PATCH 283/887] test: add full path to avoid impacting nf-test

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index b3643f48f..c747fda77 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -114,7 +114,7 @@ process {
         ]
     }
 
-    withName: 'FUSIONINSPECTOR' {
+    withName: '.*FUSIONINSPECTOR_WORKFLOW:.*:FUSIONINSPECTOR' {
         ext.when = { !params.skip_vis }
         ext.args = { ${params.fusioninspector_limitSjdbInsertNsj} != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
         ext.args2 = '--annotate --examine_coding_effect'

From 451902f49337448b8b1762226b9719a0f9611cfc Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 26 Dec 2024 19:01:20 +0000
Subject: [PATCH 284/887] test: add nf-test

---
 .../local/fusioninspector/tests/main.nf.test  | 62 +++++++++++++++++++
 1 file changed, 62 insertions(+)
 create mode 100644 modules/local/fusioninspector/tests/main.nf.test

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
new file mode 100644
index 000000000..529699dc4
--- /dev/null
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -0,0 +1,62 @@
+nextflow_process {
+
+    name "Test Process FUSIONINSPECTOR"
+    script "../main.nf"
+    process "FUSIONINSPECTOR"
+    tag "modules"
+    tag "modules_local"
+    tag "fusioninspector"
+
+    test("FUSIONINSPECTOR") {
+
+    setup {
+        run("STARFUSION_BUILD") {
+            script "/workspace/rnafusion/modules/local/starfusion/build/main.nf"
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                ]
+                input[1] = [
+                    [ id:'minigenome gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                ]
+
+                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input [3] = "human"
+                """
+            }
+        }
+    }
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                    ], // reads
+                    [
+                        file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
+                    ]
+                ]
+                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out).match() }
+            )
+        }
+
+    }
+
+
+}

From a52ce22aeaddb62d19a5241fac7e7e8eacc61648 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 26 Dec 2024 19:01:35 +0000
Subject: [PATCH 285/887] fix: emit log only and not all outputs

---
 modules/local/fusioninspector/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 9ec2c3db9..3fec42fa8 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -15,7 +15,7 @@ process FUSIONINSPECTOR {
     tuple val(meta), path("*FusionInspector.fusions.tsv")                  , emit: tsv
     tuple val(meta), path("*.coding_effect")                , optional:true, emit: tsv_coding_effect
     tuple val(meta), path("*.gtf")                          , optional:true, emit: out_gtf
-    path "*"                                                               , emit: output
+    tuple val(meta), path("*FusionInspector.log")                          , emit: log
     path "versions.yml"                                                    , emit: versions
 
     when:

From 32ee3a2485c186dbf05f29e33327270ba1ef07a4 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 26 Dec 2024 23:25:45 +0000
Subject: [PATCH 286/887] fix: update dependencies in container

---
 modules/local/fusioninspector/environment.yml | 1 +
 modules/local/fusioninspector/main.nf         | 4 ++--
 2 files changed, 3 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index 821d154d5..18677c2c2 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -6,6 +6,7 @@ dependencies:
   - bioconda::igv-reports=1.14.1
   - bioconda::perl-json-xs=4.03
   - bioconda::pysam=0.22.1
+  - conda-forge::perl-carp-assert
   - pip
   - pip:
       - intervaltree==3.1.0
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 3fec42fa8..d27953e18 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0eb08b2bd07e8012eabea34ec26304ef36b3a3bf338cdd6658ce7ab8a9c2937/data' :
-        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:d78d0c462ca32766'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/7d/7dbc3bb67499701633a925b0ed03f58ffb03d0a4c37a86a53c38ebe9a8a6cc37/data' :
+        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:b1cf35ad505b66a4'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)

From 08105922138f4bf5b5a3b54f7dee167302eb56a8 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 26 Dec 2024 23:26:19 +0000
Subject: [PATCH 287/887] test: add config and update test

---
 .../local/fusioninspector/tests/main.nf.test  |  38 ++++++
 .../fusioninspector/tests/main.nf.test.snap   | 119 ++++++++++++++++++
 .../fusioninspector/tests/nextflow.config     |   5 +
 3 files changed, 162 insertions(+)
 create mode 100644 modules/local/fusioninspector/tests/main.nf.test.snap
 create mode 100644 modules/local/fusioninspector/tests/nextflow.config

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 529699dc4..ef853b71a 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -8,6 +8,7 @@ nextflow_process {
     tag "fusioninspector"
 
     test("FUSIONINSPECTOR") {
+        config './nextflow.config'
 
     setup {
         run("STARFUSION_BUILD") {
@@ -48,6 +49,43 @@ nextflow_process {
             }
         }
 
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.tsv,
+                    process.out.tsv_coding_effect,  // TODO: These files do not seem to be produced. Consult Annick.
+                    process.out.out_gtf,            // TODO: These files do not seem to be produced. Consult Annick.
+                    file(process.out.log[0][1]).name,
+                    process.out.versions
+                    ).match() }
+            )
+        }
+
+    }
+
+    test("FUSIONINSPECTOR - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                    ], // reads
+                    [
+                        file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
+                    ]
+                ]
+                input[1] = []
+                """
+            }
+        }
+
         then {
             assertAll(
                 { assert process.success },
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
new file mode 100644
index 000000000..5a90f3745
--- /dev/null
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -0,0 +1,119 @@
+{
+    "FUSIONINSPECTOR - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.tsv.annotated.coding_effect:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "out_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tsv_coding_effect": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.tsv.annotated.coding_effect:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2024-12-26T23:13:02.858688488"
+    },
+    "FUSIONINSPECTOR": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.FusionInspector.fusions.tsv:md5,63d2b8205f7725d1c8458c642b1cde61"
+                ]
+            ],
+            [
+                
+            ],
+            [
+                
+            ],
+            "FusionInspector.log",
+            [
+                "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2024-12-26T23:12:44.532760924"
+    }
+}
\ No newline at end of file
diff --git a/modules/local/fusioninspector/tests/nextflow.config b/modules/local/fusioninspector/tests/nextflow.config
new file mode 100644
index 000000000..ca61431b2
--- /dev/null
+++ b/modules/local/fusioninspector/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'FUSIONINSPECTOR' {
+        ext.args = '--annotate --examine_coding_effect'
+    }
+}

From 8bc24f29e0c185930efe49f1dbf00104434822f6 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 12:47:00 +0000
Subject: [PATCH 288/887] docs: update changelog

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e7288298d..b2ddfa6a4 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -26,6 +26,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added a new module `CTATSPLICING_STARTOCANCERINTRONS` and a new parameter `--ctatsplicing`. This options creates reports on cancer splicing abberations and requires one or both of `--arriba` and `--starfusion` to be given. [#587](https://github.com/nf-core/rnafusion/pull/587)
 - Add parameter `--references_only` when no data should be analysed, but only the references should be built [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)
+- Add nf-test to local module: `FUSIONINSPECTOR`. [#601](https://github.com/nf-core/rnafusion/pull/601)
 
 ### Changed
 
@@ -40,6 +41,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Integrate reference building in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Move from ensembl to gencode base [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Update from ensembl 102 to gencode 46 default references [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Update`FUSIONINSPECTOR` to v2.10.0. [#601](https://github.com/nf-core/rnafusion/pull/601)
 
 ### Fixed
 

From 1545ae655b9674c89d40355888717a36340356fe Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 14:00:38 +0000
Subject: [PATCH 289/887] fix: add missing script

---
 bin/gtf2bed | 123 ++++++++++++++++++++++++++++++++++++++++++++++++++++
 1 file changed, 123 insertions(+)
 create mode 100755 bin/gtf2bed

diff --git a/bin/gtf2bed b/bin/gtf2bed
new file mode 100755
index 000000000..cfa91cf76
--- /dev/null
+++ b/bin/gtf2bed
@@ -0,0 +1,123 @@
+#!/usr/bin/env perl
+
+# Copyright (c) 2011 Erik Aronesty (erik@q32.com)
+#
+# Permission is hereby granted, free of charge, to any person obtaining a copy
+# of this software and associated documentation files (the "Software"), to deal
+# in the Software without restriction, including without limitation the rights
+# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
+# copies of the Software, and to permit persons to whom the Software is
+# furnished to do so, subject to the following conditions:
+#
+# The above copyright notice and this permission notice shall be included in
+# all copies or substantial portions of the Software.
+#
+# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
+# THE SOFTWARE.
+#
+# ALSO, IT WOULD BE NICE IF YOU LET ME KNOW YOU USED IT.
+
+use Getopt::Long;
+
+my $extended;
+GetOptions("x"=>\$extended);
+
+$in = shift @ARGV;
+
+my $in_cmd =($in =~ /\.gz$/ ? "gunzip -c $in|" : $in =~ /\.zip$/ ? "unzip -p $in|" : "$in") || die "Can't open $in: $!\n";
+open IN, $in_cmd;
+
+while (<IN>) {
+    $gff = 2 if /^##gff-version 2/;
+    $gff = 3 if /^##gff-version 3/;
+    next if /^#/ && $gff;
+
+    s/\s+$//;
+    # 0-chr 1-src 2-feat 3-beg 4-end 5-scor 6-dir 7-fram 8-attr
+    my @f = split /\t/;
+    if ($gff) {
+        # most ver 2's stick gene names in the id field
+        ($id) = $f[8]=~ /\bID="([^"]+)"/;
+        # most ver 3's stick unquoted names in the name field
+        ($id) = $f[8]=~ /\bName=([^";]+)/ if !$id && $gff == 3;
+    } else {
+        ($id) = $f[8]=~ /transcript_id "([^"]+)"/;
+    }
+
+    next unless $id && $f[0];
+
+    if ($f[2] eq 'exon') {
+        die "no position at exon on line $." if ! $f[3];
+        # gff3 puts :\d in exons sometimes
+        $id =~ s/:\d+$// if $gff == 3;
+        push @{$exons{$id}}, \@f;
+        # save lowest start
+        $trans{$id} = \@f if !$trans{$id};
+    } elsif ($f[2] eq 'start_codon') {
+        #optional, output codon start/stop as "thick" region in bed
+        $sc{$id}->[0] = $f[3];
+    } elsif ($f[2] eq 'stop_codon') {
+        $sc{$id}->[1] = $f[4];
+    } elsif ($f[2] eq 'miRNA' ) {
+        $trans{$id} = \@f if !$trans{$id};
+        push @{$exons{$id}}, \@f;
+    }
+}
+
+for $id (
+    # sort by chr then pos
+    sort {
+        $trans{$a}->[0] eq $trans{$b}->[0] ?
+        $trans{$a}->[3] <=> $trans{$b}->[3] :
+        $trans{$a}->[0] cmp $trans{$b}->[0]
+    } (keys(%trans)) ) {
+        my ($chr, undef, undef, undef, undef, undef, $dir, undef, $attr, undef, $cds, $cde) = @{$trans{$id}};
+        my ($cds, $cde);
+        ($cds, $cde) = @{$sc{$id}} if $sc{$id};
+
+        # sort by pos
+        my @ex = sort {
+            $a->[3] <=> $b->[3]
+        } @{$exons{$id}};
+
+        my $beg = $ex[0][3];
+        my $end = $ex[-1][4];
+
+        if ($dir eq '-') {
+        # swap
+        $tmp=$cds;
+        $cds=$cde;
+        $cde=$tmp;
+        $cds -= 2 if $cds;
+        $cde += 2 if $cde;
+    }
+
+    # not specified, just use exons
+    $cds = $beg if !$cds;
+    $cde = $end if !$cde;
+
+    # adjust start for bed
+    --$beg; --$cds;
+
+    my $exn = @ex;												# exon count
+    my $exst = join ",", map {$_->[3]-$beg-1} @ex;				# exon start
+    my $exsz = join ",", map {$_->[4]-$_->[3]+1} @ex;			# exon size
+
+        my $gene_id;
+        my $extend = "";
+        if ($extended) {
+            ($gene_id) = $attr =~ /gene_name "([^"]+)"/;
+            ($gene_id) = $attr =~ /gene_id "([^"]+)"/ unless $gene_id;
+            $extend="\t$gene_id";
+        }
+    # added an extra comma to make it look exactly like ucsc's beds
+    print "$chr\t$beg\t$end\t$id\t0\t$dir\t$cds\t$cde\t0\t$exn\t$exsz,\t$exst,$extend\n";
+}
+
+
+close IN;

From 0bb2fa85609f16d2b1f6b14834e829d18b588e61 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 14:00:46 +0000
Subject: [PATCH 290/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index a7e358ea0..e61cc28a6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -51,6 +51,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed bug in nf-core `RRNATRANSCRIPTS` module [#563](https://github.com/nf-core/rnafusion/issues/563)
 - Fixed bug in `GFFREAD` that caused output `gffread_fasta` not being produced [#565](https://github.com/nf-core/rnafusion/issues/565)
 - Fixed bug in `FUSIONCATCHER_DOWNLOAD` that caused an error when running with singularity profile [#573](https://github.com/nf-core/rnafusion/issues/573)
+- Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
 
 ### Removed
 

From db29ef1256e78ddf0cd9f222dd1cd2b105e5ae49 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 15:36:55 +0000
Subject: [PATCH 291/887] chore: remove unused module `bedops/convert2bed`

---
 modules.json                                  |  5 --
 .../bedops/convert2bed/environment.yml        |  5 --
 modules/nf-core/bedops/convert2bed/main.nf    | 48 -------------
 modules/nf-core/bedops/convert2bed/meta.yml   | 45 ------------
 .../bedops/convert2bed/tests/main.nf.test     | 59 ----------------
 .../convert2bed/tests/main.nf.test.snap       | 68 -------------------
 .../nf-core/bedops/convert2bed/tests/tags.yml |  2 -
 7 files changed, 232 deletions(-)
 delete mode 100644 modules/nf-core/bedops/convert2bed/environment.yml
 delete mode 100644 modules/nf-core/bedops/convert2bed/main.nf
 delete mode 100644 modules/nf-core/bedops/convert2bed/meta.yml
 delete mode 100644 modules/nf-core/bedops/convert2bed/tests/main.nf.test
 delete mode 100644 modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/bedops/convert2bed/tests/tags.yml

diff --git a/modules.json b/modules.json
index b41d5fa59..0c6c55177 100644
--- a/modules.json
+++ b/modules.json
@@ -20,11 +20,6 @@
                         "git_sha": "467c202a876d26af544fa8c4b22a050a535462a7",
                         "installed_by": ["modules"]
                     },
-                    "bedops/convert2bed": {
-                        "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
-                    },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
diff --git a/modules/nf-core/bedops/convert2bed/environment.yml b/modules/nf-core/bedops/convert2bed/environment.yml
deleted file mode 100644
index 3c13066f7..000000000
--- a/modules/nf-core/bedops/convert2bed/environment.yml
+++ /dev/null
@@ -1,5 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - "bioconda::bedops=2.4.41"
diff --git a/modules/nf-core/bedops/convert2bed/main.nf b/modules/nf-core/bedops/convert2bed/main.nf
deleted file mode 100644
index a23efbd56..000000000
--- a/modules/nf-core/bedops/convert2bed/main.nf
+++ /dev/null
@@ -1,48 +0,0 @@
-process BEDOPS_CONVERT2BED {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/bedops:2.4.41--h4ac6f70_2':
-        'biocontainers/bedops:2.4.41--h4ac6f70_2' }"
-
-    input:
-    tuple val(meta), path(in_file)
-
-    output:
-    tuple val(meta), path("*.bed"), emit: bed
-    path "versions.yml"           , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def format = in_file.getExtension()
-    """
-    convert2bed \\
-        $args \\
-        -i $format \\
-        < $in_file \\
-        > ${prefix}.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bedops/convert2bed: \$(convert2bed --version | grep vers | sed 's/^.*.version:  //')
-    END_VERSIONS
-    """
-
-    stub:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bedops/convert2bed: \$(convert2bed --version | grep vers | sed 's/^.*.version:  //')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/bedops/convert2bed/meta.yml b/modules/nf-core/bedops/convert2bed/meta.yml
deleted file mode 100644
index 6d84c0316..000000000
--- a/modules/nf-core/bedops/convert2bed/meta.yml
+++ /dev/null
@@ -1,45 +0,0 @@
-name: "bedops_convert2bed"
-description: Convert BAM/GFF/GTF/GVF/PSL files to bed
-keywords:
-  - convert
-  - bed
-  - genomics
-tools:
-  - "bedops":
-      description: "High-performance genomic feature operations."
-      homepage: "https://bedops.readthedocs.io/en/latest/content/reference/file-management/conversion/convert2bed.html#convert2bed"
-      documentation: "https://bedops.readthedocs.io/en/latest/"
-      tool_dev_url: "https://github.com/bedops"
-      doi: "10.1093/bioinformatics/bts277"
-      licence: ["GNU v2"]
-      identifier: biotools:bedops
-
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - in_file:
-        type: file
-        description: Input file
-output:
-  - bed:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. `[ id:'sample1', single_end:false ]`
-      - "*.bed":
-          type: file
-          description: Sorted BED file
-          pattern: "*.{bed}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@rannick"
-maintainers:
-  - "@rannick"
diff --git a/modules/nf-core/bedops/convert2bed/tests/main.nf.test b/modules/nf-core/bedops/convert2bed/tests/main.nf.test
deleted file mode 100644
index 3f2b193a6..000000000
--- a/modules/nf-core/bedops/convert2bed/tests/main.nf.test
+++ /dev/null
@@ -1,59 +0,0 @@
-// nf-core modules test bedops/convert2bed
-nextflow_process {
-
-    name "Test Process BEDOPS_CONVERT2BED"
-    script "../main.nf"
-    process "BEDOPS_CONVERT2BED"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "bedops"
-    tag "bedops/convert2bed"
-
-    test("sarscov2 - gtf") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gtf', checkIfExists: true)
-                    ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("sarscov2 - gtf - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gtf', checkIfExists: true)
-                    ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap b/modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap
deleted file mode 100644
index e2482924d..000000000
--- a/modules/nf-core/bedops/convert2bed/tests/main.nf.test.snap
+++ /dev/null
@@ -1,68 +0,0 @@
-{
-    "sarscov2 - gtf - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
-                ],
-                "bed": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-04-11T12:06:14.755423333"
-    },
-    "sarscov2 - gtf": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed:md5,adfdd36e848a62f4b0ea8a694abe9659"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
-                ],
-                "bed": [
-                    [
-                        {
-                            "id": "test"
-                        },
-                        "test.bed:md5,adfdd36e848a62f4b0ea8a694abe9659"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,d3d58dde1d9baba3700ff9fb5a45ce5b"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-04-11T12:06:08.876003152"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/bedops/convert2bed/tests/tags.yml b/modules/nf-core/bedops/convert2bed/tests/tags.yml
deleted file mode 100644
index fd2c29935..000000000
--- a/modules/nf-core/bedops/convert2bed/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-bedops/convert2bed:
-  - "modules/nf-core/bedops/convert2bed/**"

From 628cc842229572a212b4214fa8d25cf87dc928e4 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 15:41:20 +0000
Subject: [PATCH 292/887] fix: remove hardcoded script

---
 modules/local/fusioninspector/tests/main.nf.test | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index ef853b71a..c61e0d6ab 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -12,7 +12,7 @@ nextflow_process {
 
     setup {
         run("STARFUSION_BUILD") {
-            script "/workspace/rnafusion/modules/local/starfusion/build/main.nf"
+            script "../../starfusion/build/main.nf"
             process {
                 """
                 input[0] = [

From 856449d939a0c2563638356a3713b506194339ae Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 16:07:51 +0000
Subject: [PATCH 293/887] fix: python script does not take flags

---
 modules/local/get_rrna_transcript/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/get_rrna_transcript/main.nf b/modules/local/get_rrna_transcript/main.nf
index 5331f534a..901c17b42 100644
--- a/modules/local/get_rrna_transcript/main.nf
+++ b/modules/local/get_rrna_transcript/main.nf
@@ -20,7 +20,7 @@ process GET_RRNA_TRANSCRIPTS {
 
     script:
     """
-    $baseDir/bin/get_rrna_transcripts.py --gtf ${gtf} --output rrna.gtf
+    $baseDir/bin/get_rrna_transcripts.py ${gtf} rrna.gtf
 
     $baseDir/bin/gtf2bed rrna.gtf > rrna.bed
 

From e6f35a670d0f4313e0f050fd0d4d624894d20dcb Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 16:19:12 +0000
Subject: [PATCH 294/887] chore: add fixed version of dependencies

---
 modules/local/fusioninspector/environment.yml | 2 +-
 modules/local/fusioninspector/main.nf         | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/fusioninspector/environment.yml b/modules/local/fusioninspector/environment.yml
index 18677c2c2..26605058b 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/local/fusioninspector/environment.yml
@@ -6,7 +6,7 @@ dependencies:
   - bioconda::igv-reports=1.14.1
   - bioconda::perl-json-xs=4.03
   - bioconda::pysam=0.22.1
-  - conda-forge::perl-carp-assert
+  - conda-forge::perl-carp-assert=0.21
   - pip
   - pip:
       - intervaltree==3.1.0
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index d27953e18..cd45b6733 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/7d/7dbc3bb67499701633a925b0ed03f58ffb03d0a4c37a86a53c38ebe9a8a6cc37/data' :
-        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:b1cf35ad505b66a4'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/13/139b94a1f10c6e23a8c27eaed1e5a689db978a513d0ee155e74d35f0970814fe/data' :
+        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:c6147971d107ab11'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)

From 6ec93a2a7bac07f76ddaf7d62bce48f3b7dcb778 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 16:40:37 +0000
Subject: [PATCH 295/887] test: update tests

---
 modules/local/fusioninspector/tests/main.nf.test     |  2 +-
 .../local/fusioninspector/tests/main.nf.test.snap    | 12 ++----------
 2 files changed, 3 insertions(+), 11 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index c61e0d6ab..0189f8679 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -53,7 +53,7 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    process.out.tsv,
+                    file(process.out.tsv[0][1]).name,
                     process.out.tsv_coding_effect,  // TODO: These files do not seem to be produced. Consult Annick.
                     process.out.out_gtf,            // TODO: These files do not seem to be produced. Consult Annick.
                     file(process.out.log[0][1]).name,
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 5a90f3745..08ced813f 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -90,15 +90,7 @@
     },
     "FUSIONINSPECTOR": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.FusionInspector.fusions.tsv:md5,63d2b8205f7725d1c8458c642b1cde61"
-                ]
-            ],
+            "test.FusionInspector.fusions.tsv",
             [
                 
             ],
@@ -114,6 +106,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-26T23:12:44.532760924"
+        "timestamp": "2024-12-27T16:38:17.787825557"
     }
 }
\ No newline at end of file

From 9f656f3f752a87c9e127bf88bc5670b40d8b727f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 23:08:48 +0000
Subject: [PATCH 296/887] test: add build test

---
 conf/test_build.config        |  29 +++-
 tests/test_build.nf.test      |  40 +++++
 tests/test_build.nf.test.snap | 313 ++++++++++++++++++++++++++++++++++
 3 files changed, 380 insertions(+), 2 deletions(-)
 create mode 100644 tests/test_build.nf.test
 create mode 100644 tests/test_build.nf.test.snap

diff --git a/conf/test_build.config b/conf/test_build.config
index e577ada58..971e386ae 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -5,9 +5,28 @@
     Defines input files and everything required to run a fast and simple pipeline test.
 
     Use as follows:
-        nextflow run nf-core/rnafusion -profile test_build,<docker/singularity> --outdir <OUTDIR> -stub
+        nextflow run nf-core/rnafusion -profile test_build,<docker/singularity> --outdir <OUTDIR>
 
 ----------------------------------------------------------------------------------------
+Uses a minimal fasta and gtf for testing purposes.
+This test is designed to test the `build_references` subworkflow with the following processes on:
+- SAMTOOLS_FAIDX
+- HGNC_DOWNLOAD
+- GATK4_CREATESEQUENCEDICTIONARY
+- GET_RRNA_TRANSCRIPTS
+- GATK4_BEDTOINTERVALLIST
+- GTF_TO_REFFLAT
+- GFFREAD
+- STAR_GENOMEGENERATE
+- ARRIBA_DOWNLOAD
+- STARFUSION_BUILD
+- FUSIONREPORT_DOWNLOAD
+
+It does not test the following processes of the `build_references` subworkflow:
+- GENCODE_DOWNLOAD
+- FUSIONCATCHER_BUILD
+
+It does not test the main rnafusion subworkflows by setting references_only = true.
 */
 
 params {
@@ -18,7 +37,13 @@ params {
     references_only            = true
     input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
     no_cosmic                  = true
-    all                        = true
+    all                        = false
+    arriba                     = true
+    fusioncatcher              = false
+    starfusion                 = true
+    fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'
+    gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
+    fusionreport               = true
 
     skip_salmon_index          = true
     starfusion_build           = true
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
new file mode 100644
index 000000000..1dbda8336
--- /dev/null
+++ b/tests/test_build.nf.test
@@ -0,0 +1,40 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test_build"
+    tag "pipeline"
+    tag "pipeline_rnafusion"
+    tag "test_build"
+
+    test("Test build references") {
+
+        when {
+            params {
+                outdir               = "$outputDir"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+
+}
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
new file mode 100644
index 000000000..0b274b02a
--- /dev/null
+++ b/tests/test_build.nf.test.snap
@@ -0,0 +1,313 @@
+{
+    "Test build references": {
+        "content": [
+            12,
+            {
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": null
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "gatk4",
+                "gatk4/minigenome.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "pipeline_info",
+                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/fusion_report_db",
+                "references/fusion_report_db/DB-timestamp.txt",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/gencode",
+                "references/gencode/minigenome.fa.fai",
+                "references/gencode/minigenome.gtf.interval_list",
+                "references/gencode/minigenome.gtf.refflat",
+                "references/gffread",
+                "references/gffread/minigenome.gtf.fasta",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/salmon",
+                "references/salmon/salmon",
+                "references/salmon/salmon/complete_ref_lens.bin",
+                "references/salmon/salmon/ctable.bin",
+                "references/salmon/salmon/ctg_offsets.bin",
+                "references/salmon/salmon/duplicate_clusters.tsv",
+                "references/salmon/salmon/info.json",
+                "references/salmon/salmon/mphf.bin",
+                "references/salmon/salmon/pos.bin",
+                "references/salmon/salmon/pre_indexing.log",
+                "references/salmon/salmon/rank.bin",
+                "references/salmon/salmon/refAccumLengths.bin",
+                "references/salmon/salmon/ref_indexing.log",
+                "references/salmon/salmon/reflengths.bin",
+                "references/salmon/salmon/refseq.bin",
+                "references/salmon/salmon/seq.bin",
+                "references/salmon/salmon/versionInfo.json",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "starfusion",
+                "starfusion/ctat_genome_lib_build_dir",
+                "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz"
+            ],
+            [
+                "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
+                "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
+                "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
+                "DB-timestamp.txt:md5,119d4ae64c2c23db9ed31b4b4814f920",
+                "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a",
+                "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a",
+                "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
+                "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
+                "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
+                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
+                "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
+                "ctable.bin:md5,f66735de11dbe0b7e67eddfbfaa47755",
+                "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
+                "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
+                "info.json:md5,d91d8a954afcb4be9fe7bfb3e84b5be6",
+                "mphf.bin:md5,b364192c6c73de4c38ec2ed0750f5155",
+                "pos.bin:md5,c5890a6dd72e6c67ee90df49d62f387a",
+                "pre_indexing.log:md5,d095ca4b33aaaa1c90e1b995f3f99806",
+                "rank.bin:md5,3fe9c2dc9da7faff6362510c4f29e87d",
+                "refAccumLengths.bin:md5,26d9405e7591ec9a606caa36a094941a",
+                "ref_indexing.log:md5,f93fde6763cd03ae82686887601e9c0d",
+                "reflengths.bin:md5,187a4a0dee316c5dbf440fa3c58d9eec",
+                "refseq.bin:md5,712c4a8d988c15016ea746db7022e549",
+                "seq.bin:md5,f5cfb96c509b37c24b5f1fd5541f7a24",
+                "versionInfo.json:md5,d2c799050e81aa6e282ac8a73e773941",
+                "Genome:md5,1621486e3b854fefa37c0b4e77275233",
+                "Log.out:md5,a56a6e8003d61f86b7602ba471d042d5",
+                "SA:md5,9b6b936f3a8a1232c4fc5533daf9401d",
+                "SAindex:md5,8b2025227b78fdc83e3e5b17c0f538f7",
+                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "genomeParameters.txt:md5,15d295386ecac0ebad47f2c795fb2eb9",
+                "sjdbInfo.txt:md5,f044cb1cd38af66c832f3afc4b1655cb",
+                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+                "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+                "PFAM.domtblout.dat.gz:md5,ff17bbcb36ec03a6bd70685419e847eb",
+                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.dat.gz:md5,ba17ee9dab2dd5e2ed4f93031d61bdd0",
+                "blast_pairs.idx:md5,f8cb62c4c39b41b3a85997945fd1fab2",
+                "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                "fusion_annot_lib.idx:md5,53d8a5c7b45b673e68464867024643f7",
+                "pfam_domains.dbm:md5,030800270e0d59674f89d4774fbe492b",
+                "ref_annot.cdna.fa:md5,22cdf266a4f2f894c9b880c54df03413",
+                "ref_annot.cdna.fa.idx:md5,5f9a6293ded3d612d21b65eca948d0f2",
+                "ref_annot.cds:md5,d715b6ec2298de7227a34972395d4f23",
+                "ref_annot.cdsplus.fa:md5,b3affb83d53261f54516c108494e945d",
+                "ref_annot.cdsplus.fa.idx:md5,46fbf531aedac2d10f5e04c99eff9387",
+                "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                "ref_annot.gtf.gene_spans:md5,562cb9429e0200dd6569937dfa3c4adc",
+                "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+                "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                "ref_annot.pep:md5,7bb54b6166dbc92becadcdd20fd37a1a",
+                "ref_annot.prot_info.dbm:md5,a5b084b44d1dc0fcd9a19591c0dca7b2",
+                "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+                "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+                "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+                "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                "ref_genome.fa.nin:md5,35836935e50092d81c448ab14f39ac7b",
+                "ref_genome.fa.njs:md5,2b139dd79c628f34cc959d43bb292933",
+                "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+                "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+                "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+                "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+                "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                "Log.out:md5,bdbd36ba3a2ac0fbe0237b578d1a7bf5",
+                "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+                "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "genomeParameters.txt:md5,d2b7dcaf83a3f4f798caa97b23e6c411",
+                "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+                "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                "trans.blast.align_coords.align_coords.dbm:md5,0e4ce10a10debc6041bf62d612a87946",
+                "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2024-12-27T18:40:25.101647653"
+    }
+}
\ No newline at end of file

From aad9dc85df84a1f7cc143ac36804c1e56af1453d Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 27 Dec 2024 23:09:09 +0000
Subject: [PATCH 297/887] chore: allow to run build_references only

---
 workflows/rnafusion.nf | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 857827aa5..ee845963f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -48,6 +48,7 @@ workflow RNAFUSION {
     BUILD_REFERENCES()
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
+    if (!params.references_only) { // TODO: Remove this temporary parameter when we have a full-working GitHub nf-test
 
     //
     // QC from FASTQ files
@@ -168,6 +169,7 @@ workflow RNAFUSION {
     )
     ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
 
+    }
     //
     // Collate and save software versions
     //
@@ -210,6 +212,8 @@ workflow RNAFUSION {
             sort: true
         )
     )
+
+    if (!params.references_only) { // TODO: Remove this temporary parameter when we have a full-working GitHub nf-test
     ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
     ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
     ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
@@ -220,6 +224,7 @@ workflow RNAFUSION {
     ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
     ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
     ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
+    }
 
     MULTIQC (
         ch_multiqc_files.collect(),

From 49c69779628a751323ef97eefcd4e9690c79f02c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 30 Dec 2024 13:17:08 +0000
Subject: [PATCH 298/887] tests: update md5 ignore list

---
 tests/.nftignore | 27 +++++++++++++++++++++++++++
 1 file changed, 27 insertions(+)

diff --git a/tests/.nftignore b/tests/.nftignore
index 54792ea8f..7b5b68596 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -3,3 +3,30 @@ references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
 references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
 references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa
 references/hgnc/HGNC-DB-timestamp.txt
+references/fusion_report_db/DB-timestamp.txt
+references/salmon/salmon/ctable.bin
+references/salmon/salmon/pos.bin
+references/salmon/salmon/pre_indexing.log
+references/salmon/salmon/ref_indexing.log
+references/salmon/salmon/seq.bin
+references/star/Log.out
+starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/
+starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
+starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz
+starfusion/ctat_genome_lib_build_dir/blast_pairs.idx
+starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx
+starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm
+starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa
+starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx
+starfusion/ctat_genome_lib_build_dir/ref_annot.cds
+starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
+starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
+starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
+starfusion/ctat_genome_lib_build_dir/ref_annot.pep
+starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
+starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin
+starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs
+starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
+starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
+references/star/genomeParameters.txt
+starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt

From 98f8be17691b8c6ce54635c2e4af77734775fca8 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 30 Dec 2024 13:46:38 +0000
Subject: [PATCH 299/887] test: update nf-test

---
 tests/test_build.nf.test.snap | 28 +---------------------------
 1 file changed, 1 insertion(+), 27 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 0b274b02a..c8aefa908 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -190,7 +190,6 @@
                 "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
                 "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
-                "DB-timestamp.txt:md5,119d4ae64c2c23db9ed31b4b4814f920",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a",
                 "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a",
@@ -200,22 +199,16 @@
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
                 "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
-                "ctable.bin:md5,f66735de11dbe0b7e67eddfbfaa47755",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
                 "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
                 "info.json:md5,d91d8a954afcb4be9fe7bfb3e84b5be6",
                 "mphf.bin:md5,b364192c6c73de4c38ec2ed0750f5155",
-                "pos.bin:md5,c5890a6dd72e6c67ee90df49d62f387a",
-                "pre_indexing.log:md5,d095ca4b33aaaa1c90e1b995f3f99806",
                 "rank.bin:md5,3fe9c2dc9da7faff6362510c4f29e87d",
                 "refAccumLengths.bin:md5,26d9405e7591ec9a606caa36a094941a",
-                "ref_indexing.log:md5,f93fde6763cd03ae82686887601e9c0d",
                 "reflengths.bin:md5,187a4a0dee316c5dbf440fa3c58d9eec",
                 "refseq.bin:md5,712c4a8d988c15016ea746db7022e549",
-                "seq.bin:md5,f5cfb96c509b37c24b5f1fd5541f7a24",
                 "versionInfo.json:md5,d2c799050e81aa6e282ac8a73e773941",
                 "Genome:md5,1621486e3b854fefa37c0b4e77275233",
-                "Log.out:md5,a56a6e8003d61f86b7602ba471d042d5",
                 "SA:md5,9b6b936f3a8a1232c4fc5533daf9401d",
                 "SAindex:md5,8b2025227b78fdc83e3e5b17c0f538f7",
                 "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
@@ -225,13 +218,11 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "genomeParameters.txt:md5,15d295386ecac0ebad47f2c795fb2eb9",
                 "sjdbInfo.txt:md5,f044cb1cd38af66c832f3afc4b1655cb",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
-                "PFAM.domtblout.dat.gz:md5,ff17bbcb36ec03a6bd70685419e847eb",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -255,35 +246,20 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.dat.gz:md5,ba17ee9dab2dd5e2ed4f93031d61bdd0",
-                "blast_pairs.idx:md5,f8cb62c4c39b41b3a85997945fd1fab2",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                "fusion_annot_lib.idx:md5,53d8a5c7b45b673e68464867024643f7",
-                "pfam_domains.dbm:md5,030800270e0d59674f89d4774fbe492b",
-                "ref_annot.cdna.fa:md5,22cdf266a4f2f894c9b880c54df03413",
-                "ref_annot.cdna.fa.idx:md5,5f9a6293ded3d612d21b65eca948d0f2",
-                "ref_annot.cds:md5,d715b6ec2298de7227a34972395d4f23",
-                "ref_annot.cdsplus.fa:md5,b3affb83d53261f54516c108494e945d",
-                "ref_annot.cdsplus.fa.idx:md5,46fbf531aedac2d10f5e04c99eff9387",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                "ref_annot.gtf.gene_spans:md5,562cb9429e0200dd6569937dfa3c4adc",
                 "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                 "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                "ref_annot.pep:md5,7bb54b6166dbc92becadcdd20fd37a1a",
-                "ref_annot.prot_info.dbm:md5,a5b084b44d1dc0fcd9a19591c0dca7b2",
                 "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                 "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                 "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                 "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                "ref_genome.fa.nin:md5,35836935e50092d81c448ab14f39ac7b",
-                "ref_genome.fa.njs:md5,2b139dd79c628f34cc959d43bb292933",
                 "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                 "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                 "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                 "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                "Log.out:md5,bdbd36ba3a2ac0fbe0237b578d1a7bf5",
                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -294,13 +270,11 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "genomeParameters.txt:md5,d2b7dcaf83a3f4f798caa97b23e6c411",
                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                "trans.blast.align_coords.align_coords.dbm:md5,0e4ce10a10debc6041bf62d612a87946",
                 "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
             ]
         ],
@@ -308,6 +282,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-27T18:40:25.101647653"
+        "timestamp": "2024-12-30T13:35:33.573499693"
     }
 }
\ No newline at end of file

From abbdf2f4254b305d73e173a05962d7fbcfa8fd4b Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 30 Dec 2024 14:12:42 +0000
Subject: [PATCH 300/887] feat: add all outputs

---
 modules/local/fusioninspector/main.nf | 122 +++++++++++++++++++++++++-
 1 file changed, 120 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index d27953e18..4d7b3ad5c 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -16,6 +16,11 @@ process FUSIONINSPECTOR {
     tuple val(meta), path("*.coding_effect")                , optional:true, emit: tsv_coding_effect
     tuple val(meta), path("*.gtf")                          , optional:true, emit: out_gtf
     tuple val(meta), path("*FusionInspector.log")                          , emit: log
+    tuple val(meta), path("*html")                                         , emit: html
+    tuple val(meta), path("*abridged.tsv")                                 , emit: abridged_tsv
+    tuple val(meta), path("IGV_inputs")                                    , emit: igv_inputs
+    tuple val(meta), path("fi_workdir")                                    , emit: fi_workdir
+    tuple val(meta), path("chckpts_dir")                                   , emit: chckpts_dir
     path "versions.yml"                                                    , emit: versions
 
     when:
@@ -46,9 +51,122 @@ process FUSIONINSPECTOR {
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.FusionInspector.log
+    touch ${prefix}.FusionInspector.fusions.abridged.tsv
     touch ${prefix}.FusionInspector.fusions.tsv
-    touch ${prefix}.FusionInspector.fusions.tsv.annotated.coding_effect
-    touch ${prefix}.gtf
+    touch ${prefix}.fusion_inspector_web.html
+    mkdir -p chckpts_dir
+    touch chckpts_dir/add_FFPM.ok
+    touch chckpts_dir/add_splice_info.ok
+    touch chckpts_dir/append_microH_info.ok
+    touch chckpts_dir/blast_filter.ok
+    touch chckpts_dir/coalesce_junc_n_span.ok
+    touch chckpts_dir/cp_consol_bam.ok
+    touch chckpts_dir/cp_contigs_file_workdir
+    touch chckpts_dir/cp_final.ok
+    touch chckpts_dir/cp_gtf_file_workdir.ok
+    touch chckpts_dir/cp_tracks_json.ok
+    touch chckpts_dir/create_fi_igvjs.ok
+    touch chckpts_dir/cytoband.ok
+    touch chckpts_dir/EM_adj_counts.ok
+    touch chckpts_dir/filter_by_frag_threshs.ok
+    touch chckpts_dir/final.abridged.ok
+    touch chckpts_dir/fusion_annotator.ok
+    touch chckpts_dir/fusion_coding_region_effect.ok
+    touch chckpts_dir/fusion_contigs.ok
+    touch chckpts_dir/fusion_reports_html.ok
+    touch chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok
+    touch chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok
+    touch chckpts_dir/index_consol_bam.ok
+    touch chckpts_dir/init_EM_adj_counts.ok
+    touch chckpts_dir/init_spanning_reads_bam.ok
+    touch chckpts_dir/mark_dup_reads.ok
+    touch chckpts_dir/mark_dups_reads.index.ok
+    touch chckpts_dir/merged_contig_fai.ok
+    touch chckpts_dir/merged_contig_gtf_to_bed.ok
+    touch chckpts_dir/microH.dat.ok
+    touch chckpts_dir/prep_igv_extract_junc_reads.ok
+    touch chckpts_dir/prep_igv_junc_reads_bam.ok
+    touch chckpts_dir/prep_igv_pfam_bed.ok
+    touch chckpts_dir/prep_igv_pfam_gff3.ok
+    touch chckpts_dir/prep_igv_seqsim_bed.ok
+    touch chckpts_dir/prep_igv_seqsim_gff3.ok
+    touch chckpts_dir/prep_spanning_reads.ok
+    touch chckpts_dir/run_STAR.ok
+    touch chckpts_dir/samtools_idx_junc_reads_bam.ok
+    touch chckpts_dir/samtools_index_span_reads_bam.ok
+    touch chckpts_dir/span_reads_acc.ok
+    touch chckpts_dir/${prefix}.bed.bedsort.ok
+    touch chckpts_dir/${prefix}.bed.bgzip.ok
+    touch chckpts_dir/${prefix}.bed.tabix.ok
+    mkdir -p fi_workdir/_STARgenome
+    touch fi_workdir/Log.final.out
+    touch fi_workdir/Log.out
+    touch fi_workdir/Log.progress.out
+    touch fi_workdir/microH.dat
+    touch fi_workdir/pipeliner.456.cmds
+    touch fi_workdir/SJ.out.tab
+    touch fi_workdir/star_align.ok
+    touch fi_workdir/_STARgenome/exonGeTrInfo.tab
+    touch fi_workdir/_STARgenome/exonInfo.tab
+    touch fi_workdir/_STARgenome/geneInfo.tab
+    touch fi_workdir/_STARgenome/sjdbInfo.txt
+    touch fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab
+    touch fi_workdir/_STARgenome/sjdbList.out.tab
+    touch fi_workdir/_STARgenome/transcriptInfo.tab
+    touch fi_workdir/${prefix}.fa
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.fusion_junction_read_accs
+    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.fusion_spanning_read_accs
+    touch fi_workdir/${prefix}.gtf
+    touch fi_workdir/${prefix}.igv.Pfam.gff3
+    touch fi_workdir/${prefix}.igv.seqsimilar.gff3
+    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter
+    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj
+    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM
+    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH
+    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated
+    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.bai
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_junction_info
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_spanning_info
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.read_align_counts.idx
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok
+    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.spanning_reads_want.idx
+    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam
+    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam.bai
+    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam.bai.ok
+    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam.ok
+    mkdir -p IGV_inputs
+    touch IGV_inputs/cytoBand.txt
+    touch IGV_inputs/${prefix}.bed
+    gzip -c < /dev/null > IGV_inputs/${prefix}.bed.sorted.bed.gz
+    touch IGV_inputs/${prefix}.bed.sorted.bed.gz.tbi
+    touch IGV_inputs/${prefix}.consolidated.bam
+    touch IGV_inputs/${prefix}.consolidated.bam.bai
+    touch IGV_inputs/${prefix}.fa
+    touch IGV_inputs/${prefix}.fa.fai
+    touch IGV_inputs/${prefix}.fusion_inspector_web.json
+    touch IGV_inputs/${prefix}.gtf
+    touch IGV_inputs/${prefix}.igv.Pfam.bed
+    touch IGV_inputs/${prefix}.igv.seqsimilar.bed
+    touch IGV_inputs/${prefix}.junction_reads.bam
+    touch IGV_inputs/${prefix}.junction_reads.bam.bai
+    touch IGV_inputs/${prefix}.ROI.bed
+    touch IGV_inputs/${prefix}.spanning_reads.bam
+    touch IGV_inputs/${prefix}.spanning_reads.bam.bai
+    touch IGV_inputs/tracks.json
+    touch IGV_inputs/TrinityFusion.bed
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From c4774e5089ae55ac6c67dc456c22f312fd99ab9c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 30 Dec 2024 14:13:05 +0000
Subject: [PATCH 301/887] test: test all outputs

---
 .../local/fusioninspector/tests/main.nf.test  |   5 +
 .../fusioninspector/tests/main.nf.test.snap   | 482 +++++++++++++++++-
 2 files changed, 470 insertions(+), 17 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index ef853b71a..5f65fd9ea 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -57,6 +57,11 @@ nextflow_process {
                     process.out.tsv_coding_effect,  // TODO: These files do not seem to be produced. Consult Annick.
                     process.out.out_gtf,            // TODO: These files do not seem to be produced. Consult Annick.
                     file(process.out.log[0][1]).name,
+                    file(process.out.html[0][1]).name,
+                    process.out.abridged_tsv,
+                    process.out.igv_inputs,
+                    process.out.fi_workdir,
+                    process.out.chckpts_dir,
                     process.out.versions
                     ).match() }
             )
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 5a90f3745..59de46c49 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -12,70 +12,366 @@
                     ]
                 ],
                 "1": [
+                    
+                ],
+                "2": [
+                    
+                ],
+                "3": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.FusionInspector.fusions.tsv.annotated.coding_effect:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "2": [
+                "4": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.fusion_inspector_web.html:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "3": [
+                "5": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "4": [
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cytoBand.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.ROI.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.bed.sorted.bed.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.bed.sorted.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.consolidated.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.consolidated.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.fusion_inspector_web.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.junction_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.junction_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.spanning_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.spanning_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "tracks.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
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+                "igv_inputs": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "cytoBand.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.ROI.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.bed.sorted.bed.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.bed.sorted.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.consolidated.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.consolidated.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.fusion_inspector_web.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.junction_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.junction_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.spanning_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "test.spanning_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "tracks.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "log": [
                     [
                         {
                             "id": "test",
                             "single_end": false
                         },
-                        "test.FusionInspector.fusions.tsv.annotated.coding_effect:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
+                ],
+                "out_gtf": [
+                    
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tsv_coding_effect": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
@@ -86,7 +382,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-26T23:13:02.858688488"
+        "timestamp": "2024-12-30T14:11:28.656578568"
     },
     "FUSIONINSPECTOR": {
         "content": [
@@ -96,7 +392,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.tsv:md5,63d2b8205f7725d1c8458c642b1cde61"
+                    "test.FusionInspector.fusions.tsv:md5,d460c0d7fa555fcf0db9615228b4c90f"
                 ]
             ],
             [
@@ -106,6 +402,158 @@
                 
             ],
             "FusionInspector.log",
+            "test.fusion_inspector_web.html",
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.FusionInspector.fusions.abridged.tsv:md5,4ea7d11c65f33f963f0c87559d8c51b2"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "cytoBand.txt:md5,d8a84202fa42d9455222c94ae641c8b4",
+                        "test.ROI.bed:md5,671de301af27b3dab40ee224401d3a0e",
+                        "test.bed:md5,f3263827da9544f59c67700341086dd8",
+                        "test.bed.sorted.bed.gz:md5,a306900912cc4479f06d61bea00a3ea6",
+                        "test.bed.sorted.bed.gz.tbi:md5,8c9d524558ade2b82c67d60bcfa0de6b",
+                        "test.consolidated.bam:md5,8744a27f874ad01ee159d588a482f29d",
+                        "test.consolidated.bam.bai:md5,07b2313770d96851c54b224daeb9f5eb",
+                        "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
+                        "test.fa.fai:md5,15ddfba09f4abf8a15de5eea6c6286ee",
+                        "test.fusion_inspector_web.json:md5,ebe4faf6b6133b40d6c2847195cf55a9",
+                        "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
+                        "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.junction_reads.bam:md5,914400e910a2ff23e0daf9326a81700e",
+                        "test.junction_reads.bam.bai:md5,0b6a1078fb91a6d4b9591324b0f95649",
+                        "test.spanning_reads.bam:md5,7a328ec72821a97f2d38f76a642a507e",
+                        "test.spanning_reads.bam.bai:md5,962a587d2c752d7762a511fd02bca62e",
+                        "tracks.json:md5,72f2234c2ad2b7997226283acf040161"
+                    ]
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "Log.final.out:md5,858dea4a306e57423b12cb2fcfe3b7fd",
+                        "Log.out:md5,28f71667674309af6a6017d357255bad",
+                        "Log.progress.out:md5,b2bd061d6cbaaf3d6d3b1fed547f69b8",
+                        "SJ.out.tab:md5,2ed3417c0ef9daa230a72e6fd3137588",
+                        [
+                            "exonGeTrInfo.tab:md5,8815dc1fce3a5f6cb836ea8f822e9c81",
+                            "exonInfo.tab:md5,e51c649a0ca61719e8d4f7324c069c60",
+                            "geneInfo.tab:md5,2f334fb0772c906860c9489ee0d8f3ff",
+                            "sjdbInfo.txt:md5,3a27e9e4b5f402d3daed85dd6f06519e",
+                            "sjdbList.fromGTF.out.tab:md5,fd4e89499ee467de647bd202d8894628",
+                            "sjdbList.out.tab:md5,dad82973249f1c094e36610d2663ddec",
+                            "transcriptInfo.tab:md5,69658043a209e2bb59c1da71b11fd5c3"
+                        ],
+                        "microH.dat:md5,153277f4c2b5600b70fea4508255b20b",
+                        "pipeliner.456.cmds:md5,a057d47f4b3903f9c2b363016f909314",
+                        "star_align.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
+                        "test.fusion_preds.coalesced.summary:md5,6aea1d2c5e0e916b0c9cfa2bf233e9aa",
+                        "test.fusion_preds.coalesced.summary.EMadj:md5,930f235f0915af2c35af3e75e451e464",
+                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh:md5,47bcd82c0ffca6b64c64bb9edca549e0",
+                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo:md5,6bb11f5efe7bb3879950a492a078d21b",
+                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter:md5,5cd1259a3279026bb5e50c2a4890e548",
+                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d",
+                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter:md5,5cd1259a3279026bb5e50c2a4890e548",
+                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d",
+                        "test.fusion_preds.coalesced.summary.fusion_junction_read_accs:md5,9b872a5f1e6a5a21957a55fbf758cbef",
+                        "test.fusion_preds.coalesced.summary.fusion_spanning_read_accs:md5,5475c0ed243fbeb67f5a3e3dd25869ce",
+                        "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
+                        "test.igv.Pfam.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.igv.seqsimilar.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.post_blast_and_promiscuity_filter:md5,5cd1259a3279026bb5e50c2a4890e548",
+                        "test.post_blast_and_promiscuity_filter.EMadj:md5,d0d7849496bb1579ca5bc6e87c8b2921",
+                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM:md5,0adc8c263fe9f596ff52111b0a7f6027",
+                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH:md5,ac3f6599a5f01beec0ac9ed8e1837e31",
+                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated:md5,31bd0b83ec77de19a5b38467ef22957b",
+                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect:md5,d460c0d7fa555fcf0db9615228b4c90f",
+                        "test.star.cSorted.dupsMarked.bam:md5,8744a27f874ad01ee159d588a482f29d",
+                        "test.star.cSorted.dupsMarked.bam.bai:md5,07b2313770d96851c54b224daeb9f5eb",
+                        "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis:md5,a1a0daca698e0ff4141f820c9fe64360",
+                        "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam:md5,7585d76d73eacfc222ce76cdb4e22d0c",
+                        "test.star.cSorted.dupsMarked.bam.fusion_junction_info:md5,3a56cfc29fee2bdb0171adf0d0df5829",
+                        "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam:md5,c9e99bbe81bdbadf5fa56176828540ae",
+                        "test.star.cSorted.dupsMarked.bam.fusion_spanning_info:md5,d92895bbc30299eb0cdcc8669e3cd9ff",
+                        "test.star.cSorted.dupsMarked.bam.read_align_counts.idx:md5,e08aab8e6ce7ebcadb2554b8336e0fd1",
+                        "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx:md5,15c9b83f36e30cf6f8fe039a2597e597",
+                        "test.star.sortedByCoord.out.bam:md5,5e933b7b3914d249988094953f01e67c",
+                        "test.star.sortedByCoord.out.bam.bai:md5,76c0b5b0ff34a612afbc2b5f684653a2",
+                        "test.star.sortedByCoord.out.bam.bai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.star.sortedByCoord.out.bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "add_FFPM.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "add_splice_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "append_microH_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "blast_filter.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "coalesce_junc_n_span.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "cp_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "cp_contigs_file_workdir:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "cp_final.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "cp_gtf_file_workdir.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "cp_tracks_json.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "create_fi_igvjs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "cytoband.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "filter_by_frag_threshs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "final.abridged.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_annotator.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_coding_region_effect.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_contigs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "fusion_reports_html.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "get_fusion_JUNCTION_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "get_fusion_SPANNING_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "index_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "init_EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "init_spanning_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "mark_dup_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "mark_dups_reads.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "merged_contig_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "merged_contig_gtf_to_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "microH.dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "prep_igv_extract_junc_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "prep_igv_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "prep_igv_pfam_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "prep_igv_pfam_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "prep_igv_seqsim_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "prep_igv_seqsim_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "prep_spanning_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "run_STAR.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "samtools_idx_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "samtools_index_span_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "span_reads_acc.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.bed.bedsort.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.bed.bgzip.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                        "test.bed.tabix.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            ],
             [
                 "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
             ]
@@ -114,6 +562,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-26T23:12:44.532760924"
+        "timestamp": "2024-12-30T14:11:16.244098341"
     }
 }
\ No newline at end of file

From e4e64e25b0e798e0383af1b861607fbb5da4d81c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 30 Dec 2024 18:26:44 +0000
Subject: [PATCH 302/887] test: add test_build to ci

---
 .github/workflows/ci.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index a510d72db..71ff72459 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -39,6 +39,7 @@ jobs:
           - "latest-stable"
         test_profile:
           - "test_stub"
+          - "test_build"
         compute_profile:
           - "docker"
           - "singularity"

From e631ca1a18f34c21cd3894ff92194443d905dac9 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 30 Dec 2024 23:49:43 +0000
Subject: [PATCH 303/887] test: update nf-test to capture stable and unstable
 files

---
 .../local/fusioninspector/tests/main.nf.test  | 105 +++++++++---------
 1 file changed, 54 insertions(+), 51 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index a331515cf..4ac373178 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -10,26 +10,26 @@ nextflow_process {
     test("FUSIONINSPECTOR") {
         config './nextflow.config'
 
-    setup {
-        run("STARFUSION_BUILD") {
-            script "../../starfusion/build/main.nf"
-            process {
-                """
-                input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
-                ]
-                input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
-                ]
+        setup {
+            run("STARFUSION_BUILD") {
+                script "../../starfusion/build/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'minigenome fasta' ],
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                    ]
+                    input[1] = [
+                        [ id:'minigenome gtf' ],
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                    ]
 
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
-                """
+                    input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                    input[3] = "human"
+                    """
+                }
             }
         }
-    }
 
         when {
             process {
@@ -50,6 +50,38 @@ nextflow_process {
         }
 
         then {
+
+            def unstableFiles = [
+                "test.bed",
+                "test.consolidated.bam",
+                "test.junction_reads.bam",
+                "test.spanning_reads.bam",
+                "Log.final.out",
+                "Log.out",
+                "microH.dat",
+                "pipeliner.456.cmds",
+                "test.fusion_preds.coalesced.summary",
+                "test.fusion_preds.coalesced.summary.EMadj",
+                "test.fusion_preds.coalesced.summary.min_frag_thresh",
+                "test.fusion_preds.coalesced.summary.wSpliceInfo",
+                "test.fusion_preds.coalesced.summary.wSpliceInfo.post_blast_filter",
+                "test.fusion_preds.coalesced.summary.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "test.fusion_preds.coalesced.summary.fusion_junction_reads_acc",
+                "test.fusion_preds.coalesced.summary.fusion_spanning_reads_acc",
+                "test.post_blast_and_promiscuity_filter",
+                "test.post_blast_and_promiscuity_filter.EMadj",
+                "test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "test.star.cSorted.dupsMarked.bam",
+                "test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "test.star.cSorted.dupsMarked.bam.read_align_counts",
+                "test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "test.star.sortedByCoord.out.bam",
+                "test.star.sortedByCoord.out.bam.bai"
+            ]
+
             assertAll(
                 { assert process.success },
                 { assert snapshot(
@@ -59,47 +91,18 @@ nextflow_process {
                     file(process.out.log[0][1]).name,
                     file(process.out.html[0][1]).name,
                     process.out.abridged_tsv,
-                    process.out.igv_inputs,
-                    process.out.fi_workdir,
+                    file(process.out.igv_inputs[0][1]).listFiles().findAll { file -> !unstableFiles.contains(file.name) },
+                    file(process.out.fi_workdir[0][1]).listFiles().findAll { file -> !unstableFiles.contains(file.name) },
+                    file(process.out.igv_inputs[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
+                    file(process.out.igv_inputs[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
                     process.out.chckpts_dir,
                     process.out.versions
-                    ).match() }
+                    ).match() },
             )
         }
 
     }
 
-    test("FUSIONINSPECTOR - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                    [
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
-                    ], // reads
-                    [
-                        file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
-                    ]
-                ]
-                input[1] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    process.out).match() }
-            )
-        }
-
-    }
 
 
 }

From 0f0d5cf8f42b552dd9d29979fd281278609e5e7f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 31 Dec 2024 14:56:46 +0000
Subject: [PATCH 304/887] test: update test and snapshot

---
 .../local/fusioninspector/tests/main.nf.test  |  49 ++++-
 .../fusioninspector/tests/main.nf.test.snap   | 191 ++++++++----------
 2 files changed, 125 insertions(+), 115 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 4ac373178..26a7ca6d9 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -54,12 +54,13 @@ nextflow_process {
             def unstableFiles = [
                 "test.bed",
                 "test.consolidated.bam",
+                "test.consolidated.bam.bai",
                 "test.junction_reads.bam",
+                "test.junction_reads.bam.bai",
                 "test.spanning_reads.bam",
                 "Log.final.out",
                 "Log.out",
                 "microH.dat",
-                "pipeliner.456.cmds",
                 "test.fusion_preds.coalesced.summary",
                 "test.fusion_preds.coalesced.summary.EMadj",
                 "test.fusion_preds.coalesced.summary.min_frag_thresh",
@@ -75,11 +76,20 @@ nextflow_process {
                 "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
                 "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
                 "test.star.cSorted.dupsMarked.bam",
+                "test.star.cSorted.dupsMarked.bam.bai",
+                "test.star.cSorted.dupsMarked.bam.fusion_junction_info",
                 "test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
                 "test.star.cSorted.dupsMarked.bam.read_align_counts",
+                "test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
                 "test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
                 "test.star.sortedByCoord.out.bam",
-                "test.star.sortedByCoord.out.bam.bai"
+                "test.star.sortedByCoord.out.bam.bai",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter"
             ]
 
             assertAll(
@@ -90,19 +100,48 @@ nextflow_process {
                     process.out.out_gtf,            // TODO: These files do not seem to be produced. Consult Annick.
                     file(process.out.log[0][1]).name,
                     file(process.out.html[0][1]).name,
-                    process.out.abridged_tsv,
+                    file(process.out.abridged_tsv[0][1]).name,
                     file(process.out.igv_inputs[0][1]).listFiles().findAll { file -> !unstableFiles.contains(file.name) },
                     file(process.out.fi_workdir[0][1]).listFiles().findAll { file -> !unstableFiles.contains(file.name) },
                     file(process.out.igv_inputs[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
-                    file(process.out.igv_inputs[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
+                    file(process.out.fi_workdir[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
                     process.out.chckpts_dir,
                     process.out.versions
-                    ).match() },
+                    ).match() }
             )
         }
 
     }
 
+    test("FUSIONINSPECTOR stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                    ], // reads
+                    [
+                        file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
+                    ]
+                ]
+                input[1] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out
+                    ).match() }
+            )
+        }
 
+    }
 
 }
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index f338dd55a..6e3f05c2c 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "FUSIONINSPECTOR - stub": {
+    "FUSIONINSPECTOR stub": {
         "content": [
             {
                 "0": [
@@ -12,10 +12,10 @@
                     ]
                 ],
                 "1": [
-
+                    
                 ],
                 "2": [
-
+                    
                 ],
                 "3": [
                     [
@@ -359,7 +359,7 @@
                     ]
                 ],
                 "out_gtf": [
-
+                    
                 ],
                 "tsv": [
                     [
@@ -371,7 +371,7 @@
                     ]
                 ],
                 "tsv_coding_effect": [
-
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
@@ -382,124 +382,95 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-30T14:11:28.656578568"
+        "timestamp": "2024-12-31T13:48:13.020571472"
     },
     "FUSIONINSPECTOR": {
         "content": [
+            "test.FusionInspector.fusions.tsv",
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.FusionInspector.fusions.tsv:md5,d460c0d7fa555fcf0db9615228b4c90f"
-                ]
-            ],
-            [
-
+                
             ],
             [
-
+                
             ],
             "FusionInspector.log",
             "test.fusion_inspector_web.html",
+            "test.FusionInspector.fusions.abridged.tsv",
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.FusionInspector.fusions.abridged.tsv:md5,4ea7d11c65f33f963f0c87559d8c51b2"
-                ]
+                "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
+                "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
+                "test.fa.fai:md5,15ddfba09f4abf8a15de5eea6c6286ee",
+                "cytoBand.txt:md5,d8a84202fa42d9455222c94ae641c8b4",
+                "test.bed.sorted.bed.gz:md5,a306900912cc4479f06d61bea00a3ea6",
+                "test.bed.sorted.bed.gz.tbi:md5,8c9d524558ade2b82c67d60bcfa0de6b",
+                "test.spanning_reads.bam.bai:md5,6e035943a5c90026bd499e78c7eeed4a",
+                "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusion_inspector_web.json:md5,ebe4faf6b6133b40d6c2847195cf55a9",
+                "test.ROI.bed:md5,671de301af27b3dab40ee224401d3a0e",
+                "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "tracks.json:md5,72f2234c2ad2b7997226283acf040161"
             ],
             [
+                "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
+                "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
+                "pipeliner.456.cmds:md5,bb8bf0e749f794dfb37efc2ff7c24be0",
+                "Log.progress.out:md5,b2bd061d6cbaaf3d6d3b1fed547f69b8",
                 [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    [
-                        "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "cytoBand.txt:md5,d8a84202fa42d9455222c94ae641c8b4",
-                        "test.ROI.bed:md5,671de301af27b3dab40ee224401d3a0e",
-                        "test.bed:md5,f3263827da9544f59c67700341086dd8",
-                        "test.bed.sorted.bed.gz:md5,a306900912cc4479f06d61bea00a3ea6",
-                        "test.bed.sorted.bed.gz.tbi:md5,8c9d524558ade2b82c67d60bcfa0de6b",
-                        "test.consolidated.bam:md5,8744a27f874ad01ee159d588a482f29d",
-                        "test.consolidated.bam.bai:md5,07b2313770d96851c54b224daeb9f5eb",
-                        "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
-                        "test.fa.fai:md5,15ddfba09f4abf8a15de5eea6c6286ee",
-                        "test.fusion_inspector_web.json:md5,ebe4faf6b6133b40d6c2847195cf55a9",
-                        "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
-                        "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.junction_reads.bam:md5,914400e910a2ff23e0daf9326a81700e",
-                        "test.junction_reads.bam.bai:md5,0b6a1078fb91a6d4b9591324b0f95649",
-                        "test.spanning_reads.bam:md5,7a328ec72821a97f2d38f76a642a507e",
-                        "test.spanning_reads.bam.bai:md5,962a587d2c752d7762a511fd02bca62e",
-                        "tracks.json:md5,72f2234c2ad2b7997226283acf040161"
-                    ]
-                ]
+                    "exonGeTrInfo.tab:md5,8815dc1fce3a5f6cb836ea8f822e9c81",
+                    "exonInfo.tab:md5,e51c649a0ca61719e8d4f7324c069c60",
+                    "geneInfo.tab:md5,2f334fb0772c906860c9489ee0d8f3ff",
+                    "sjdbInfo.txt:md5,3a27e9e4b5f402d3daed85dd6f06519e",
+                    "sjdbList.fromGTF.out.tab:md5,fd4e89499ee467de647bd202d8894628",
+                    "sjdbList.out.tab:md5,dad82973249f1c094e36610d2663ddec",
+                    "transcriptInfo.tab:md5,69658043a209e2bb59c1da71b11fd5c3"
+                ],
+                "SJ.out.tab:md5,2ed3417c0ef9daa230a72e6fd3137588",
+                "star_align.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.star.sortedByCoord.out.bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.star.sortedByCoord.out.bam.bai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam:md5,7585d76d73eacfc222ce76cdb4e22d0c",
+                "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam:md5,c9e99bbe81bdbadf5fa56176828540ae",
+                "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis:md5,a1a0daca698e0ff4141f820c9fe64360",
+                "test.igv.Pfam.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.igv.seqsimilar.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d"
             ],
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    [
-                        "Log.final.out:md5,858dea4a306e57423b12cb2fcfe3b7fd",
-                        "Log.out:md5,28f71667674309af6a6017d357255bad",
-                        "Log.progress.out:md5,b2bd061d6cbaaf3d6d3b1fed547f69b8",
-                        "SJ.out.tab:md5,2ed3417c0ef9daa230a72e6fd3137588",
-                        [
-                            "exonGeTrInfo.tab:md5,8815dc1fce3a5f6cb836ea8f822e9c81",
-                            "exonInfo.tab:md5,e51c649a0ca61719e8d4f7324c069c60",
-                            "geneInfo.tab:md5,2f334fb0772c906860c9489ee0d8f3ff",
-                            "sjdbInfo.txt:md5,3a27e9e4b5f402d3daed85dd6f06519e",
-                            "sjdbList.fromGTF.out.tab:md5,fd4e89499ee467de647bd202d8894628",
-                            "sjdbList.out.tab:md5,dad82973249f1c094e36610d2663ddec",
-                            "transcriptInfo.tab:md5,69658043a209e2bb59c1da71b11fd5c3"
-                        ],
-                        "microH.dat:md5,153277f4c2b5600b70fea4508255b20b",
-                        "pipeliner.456.cmds:md5,a057d47f4b3903f9c2b363016f909314",
-                        "star_align.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
-                        "test.fusion_preds.coalesced.summary:md5,6aea1d2c5e0e916b0c9cfa2bf233e9aa",
-                        "test.fusion_preds.coalesced.summary.EMadj:md5,930f235f0915af2c35af3e75e451e464",
-                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh:md5,47bcd82c0ffca6b64c64bb9edca549e0",
-                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo:md5,6bb11f5efe7bb3879950a492a078d21b",
-                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter:md5,5cd1259a3279026bb5e50c2a4890e548",
-                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d",
-                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter:md5,5cd1259a3279026bb5e50c2a4890e548",
-                        "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d",
-                        "test.fusion_preds.coalesced.summary.fusion_junction_read_accs:md5,9b872a5f1e6a5a21957a55fbf758cbef",
-                        "test.fusion_preds.coalesced.summary.fusion_spanning_read_accs:md5,5475c0ed243fbeb67f5a3e3dd25869ce",
-                        "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
-                        "test.igv.Pfam.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.igv.seqsimilar.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.post_blast_and_promiscuity_filter:md5,5cd1259a3279026bb5e50c2a4890e548",
-                        "test.post_blast_and_promiscuity_filter.EMadj:md5,d0d7849496bb1579ca5bc6e87c8b2921",
-                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM:md5,0adc8c263fe9f596ff52111b0a7f6027",
-                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH:md5,ac3f6599a5f01beec0ac9ed8e1837e31",
-                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated:md5,31bd0b83ec77de19a5b38467ef22957b",
-                        "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect:md5,d460c0d7fa555fcf0db9615228b4c90f",
-                        "test.star.cSorted.dupsMarked.bam:md5,8744a27f874ad01ee159d588a482f29d",
-                        "test.star.cSorted.dupsMarked.bam.bai:md5,07b2313770d96851c54b224daeb9f5eb",
-                        "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis:md5,a1a0daca698e0ff4141f820c9fe64360",
-                        "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam:md5,7585d76d73eacfc222ce76cdb4e22d0c",
-                        "test.star.cSorted.dupsMarked.bam.fusion_junction_info:md5,3a56cfc29fee2bdb0171adf0d0df5829",
-                        "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam:md5,c9e99bbe81bdbadf5fa56176828540ae",
-                        "test.star.cSorted.dupsMarked.bam.fusion_spanning_info:md5,d92895bbc30299eb0cdcc8669e3cd9ff",
-                        "test.star.cSorted.dupsMarked.bam.read_align_counts.idx:md5,e08aab8e6ce7ebcadb2554b8336e0fd1",
-                        "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx:md5,15c9b83f36e30cf6f8fe039a2597e597",
-                        "test.star.sortedByCoord.out.bam:md5,5e933b7b3914d249988094953f01e67c",
-                        "test.star.sortedByCoord.out.bam.bai:md5,76c0b5b0ff34a612afbc2b5f684653a2",
-                        "test.star.sortedByCoord.out.bam.bai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.star.sortedByCoord.out.bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ]
+                "test.fa",
+                "test.gtf",
+                "test.fa.fai",
+                "cytoBand.txt",
+                "test.bed.sorted.bed.gz",
+                "test.bed.sorted.bed.gz.tbi",
+                "test.spanning_reads.bam.bai",
+                "test.igv.Pfam.bed",
+                "test.igv.seqsimilar.bed",
+                "test.fusion_inspector_web.json",
+                "test.ROI.bed",
+                "TrinityFusion.bed",
+                "tracks.json"
+            ],
+            [
+                "test.fa",
+                "test.gtf",
+                "pipeliner.456.cmds",
+                "Log.progress.out",
+                "_STARgenome",
+                "SJ.out.tab",
+                "star_align.ok",
+                "test.star.sortedByCoord.out.bam.ok",
+                "test.star.sortedByCoord.out.bam.bai.ok",
+                "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "test.igv.Pfam.gff3",
+                "test.igv.seqsimilar.gff3",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info"
             ],
             [
                 [
@@ -562,6 +533,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-30T14:11:16.244098341"
+        "timestamp": "2024-12-31T14:34:37.759837761"
     }
-}
+}
\ No newline at end of file

From 99f409f57f2f64addfe718d2438d8051ca1bea3f Mon Sep 17 00:00:00 2001
From: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 2 Jan 2025 14:05:29 +0100
Subject: [PATCH 305/887] chore: add indentation

---
 workflows/rnafusion.nf | 232 ++++++++++++++++++++---------------------
 1 file changed, 116 insertions(+), 116 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ee845963f..ea0340f6e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -50,124 +50,124 @@ workflow RNAFUSION {
 
     if (!params.references_only) { // TODO: Remove this temporary parameter when we have a full-working GitHub nf-test
 
-    //
-    // QC from FASTQ files
-    //
-    FASTQC (
-        ch_samplesheet
-    )
-    ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-    ch_versions = ch_versions.mix(FASTQC.out.versions)
-
-
-    //
-    // Trimming
-    //
-    TRIM_WORKFLOW (
-        ch_samplesheet,
-        Channel.value(params.adapter_fasta),
-        params.fastp_trim
-    )
-    ch_reads = TRIM_WORKFLOW.out.ch_reads_all
-    ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
-
-    SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
-    ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
-    ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
+        //
+        // QC from FASTQ files
+        //
+        FASTQC (
+            ch_samplesheet
+        )
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+        ch_versions = ch_versions.mix(FASTQC.out.versions)
 
 
-    //
-    // SUBWORKFLOW:  Run STAR alignment and Arriba
-    //
-
-    // TODO: add params.seq_platform and pass it as argument to arriba_workflow
-    // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
-    // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
-
-    ARRIBA_WORKFLOW (
-        ch_reads,
-        BUILD_REFERENCES.out.ch_gtf,
-        BUILD_REFERENCES.out.ch_fasta,
-        BUILD_REFERENCES.out.ch_starindex_ref,
-        BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
-        BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
-        BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
-        BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
-        BUILD_REFERENCES.out.ch_starfusion_ref,
-        params.arriba,                   // boolean
-        params.all,                      // boolean
-        params.fusioninspector_only,     // boolean
-        params.star_ignore_sjdbgtf,      // boolean
-        params.ctatsplicing,             // boolean
-        params.seq_center ?: '',         // string
-        params.arriba_fusions,           // path
-        params.cram                      // array
-    )
-    ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
+        //
+        // Trimming
+        //
+        TRIM_WORKFLOW (
+            ch_samplesheet,
+            Channel.value(params.adapter_fasta),
+            params.fastp_trim
+        )
+        ch_reads = TRIM_WORKFLOW.out.ch_reads_all
+        ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+
+        SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
+        ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
+        ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
+
+
+        //
+        // SUBWORKFLOW:  Run STAR alignment and Arriba
+        //
+
+        // TODO: add params.seq_platform and pass it as argument to arriba_workflow
+        // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
+        // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
+
+        ARRIBA_WORKFLOW (
+            ch_reads,
+            BUILD_REFERENCES.out.ch_gtf,
+            BUILD_REFERENCES.out.ch_fasta,
+            BUILD_REFERENCES.out.ch_starindex_ref,
+            BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
+            BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
+            BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
+            BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
+            BUILD_REFERENCES.out.ch_starfusion_ref,
+            params.arriba,                   // boolean
+            params.all,                      // boolean
+            params.fusioninspector_only,     // boolean
+            params.star_ignore_sjdbgtf,      // boolean
+            params.ctatsplicing,             // boolean
+            params.seq_center ?: '',         // string
+            params.arriba_fusions,           // path
+            params.cram                      // array
+        )
+        ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
 
 
-    //Run STAR fusion
-    STARFUSION_WORKFLOW (
-        ch_reads,
-        BUILD_REFERENCES.out.ch_gtf,
-        BUILD_REFERENCES.out.ch_starindex_ref,
-        BUILD_REFERENCES.out.ch_fasta,
-        BUILD_REFERENCES.out.ch_starfusion_ref
-    )
-    ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+        //Run STAR fusion
+        STARFUSION_WORKFLOW (
+            ch_reads,
+            BUILD_REFERENCES.out.ch_gtf,
+            BUILD_REFERENCES.out.ch_starindex_ref,
+            BUILD_REFERENCES.out.ch_fasta,
+            BUILD_REFERENCES.out.ch_starfusion_ref
+        )
+        ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
 
 
-    //Run fusioncatcher
-    FUSIONCATCHER_WORKFLOW (
-        ch_reads
-    )
-    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+        //Run fusioncatcher
+        FUSIONCATCHER_WORKFLOW (
+            ch_reads
+        )
+        ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
 
 
-    //Run stringtie
-    STRINGTIE_WORKFLOW (
-        STARFUSION_WORKFLOW.out.ch_bam_sorted,
-        BUILD_REFERENCES.out.ch_gtf
-    )
-    ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
+        //Run stringtie
+        STRINGTIE_WORKFLOW (
+            STARFUSION_WORKFLOW.out.ch_bam_sorted,
+            BUILD_REFERENCES.out.ch_gtf
+        )
+        ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
 
 
-    //Run fusion-report
-    FUSIONREPORT_WORKFLOW (
-        ch_reads,
-        BUILD_REFERENCES.out.ch_fusionreport_ref,
-        ARRIBA_WORKFLOW.out.fusions,
-        STARFUSION_WORKFLOW.out.fusions,
-        FUSIONCATCHER_WORKFLOW.out.fusions
-    )
-    ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
-
-    //Run fusionInpector
-    FUSIONINSPECTOR_WORKFLOW (
-        ch_reads,
-        FUSIONREPORT_WORKFLOW.out.fusion_list,
-        FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
-        FUSIONREPORT_WORKFLOW.out.report,
-        FUSIONREPORT_WORKFLOW.out.csv,
-        STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
-        BUILD_REFERENCES.out.ch_gtf,
-        BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
-        BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
-        BUILD_REFERENCES.out.ch_hgnc_ref,
-        BUILD_REFERENCES.out.ch_hgnc_date
-    )
-    ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
+        //Run fusion-report
+        FUSIONREPORT_WORKFLOW (
+            ch_reads,
+            BUILD_REFERENCES.out.ch_fusionreport_ref,
+            ARRIBA_WORKFLOW.out.fusions,
+            STARFUSION_WORKFLOW.out.fusions,
+            FUSIONCATCHER_WORKFLOW.out.fusions
+        )
+        ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
+
+        //Run fusionInpector
+        FUSIONINSPECTOR_WORKFLOW (
+            ch_reads,
+            FUSIONREPORT_WORKFLOW.out.fusion_list,
+            FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
+            FUSIONREPORT_WORKFLOW.out.report,
+            FUSIONREPORT_WORKFLOW.out.csv,
+            STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+            BUILD_REFERENCES.out.ch_gtf,
+            BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
+            BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
+            BUILD_REFERENCES.out.ch_hgnc_ref,
+            BUILD_REFERENCES.out.ch_hgnc_date
+        )
+        ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
 
 
-    //QC
-    QC_WORKFLOW (
-        STARFUSION_WORKFLOW.out.ch_bam_sorted,
-        BUILD_REFERENCES.out.ch_refflat,
-        BUILD_REFERENCES.out.ch_fasta,
-        BUILD_REFERENCES.out.ch_fai,
-        BUILD_REFERENCES.out.ch_rrna_interval
-    )
-    ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
+        //QC
+        QC_WORKFLOW (
+            STARFUSION_WORKFLOW.out.ch_bam_sorted,
+            BUILD_REFERENCES.out.ch_refflat,
+            BUILD_REFERENCES.out.ch_fasta,
+            BUILD_REFERENCES.out.ch_fai,
+            BUILD_REFERENCES.out.ch_rrna_interval
+        )
+        ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
 
     }
     //
@@ -214,16 +214,16 @@ workflow RNAFUSION {
     )
 
     if (!params.references_only) { // TODO: Remove this temporary parameter when we have a full-working GitHub nf-test
-    ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
-    ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
     }
 
     MULTIQC (

From 36960ec12838701e7518aef648f4a823ec868c97 Mon Sep 17 00:00:00 2001
From: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 2 Jan 2025 15:23:33 +0100
Subject: [PATCH 306/887] test: add nf-test

---
 .../main.nf}                                  |  2 +-
 .../fusionreport_workflow/tests/main.nf.test  | 73 +++++++++++++++++++
 2 files changed, 74 insertions(+), 1 deletion(-)
 rename subworkflows/local/{fusionreport_workflow.nf => fusionreport_workflow/main.nf} (94%)
 create mode 100644 subworkflows/local/fusionreport_workflow/tests/main.nf.test

diff --git a/subworkflows/local/fusionreport_workflow.nf b/subworkflows/local/fusionreport_workflow/main.nf
similarity index 94%
rename from subworkflows/local/fusionreport_workflow.nf
rename to subworkflows/local/fusionreport_workflow/main.nf
index 04019e121..eac40e328 100644
--- a/subworkflows/local/fusionreport_workflow.nf
+++ b/subworkflows/local/fusionreport_workflow/main.nf
@@ -1,4 +1,4 @@
-include { FUSIONREPORT      }     from '../../modules/local/fusionreport/detect/main'
+include { FUSIONREPORT      }     from '../../../modules/local/fusionreport/detect/main'
 
 
 workflow FUSIONREPORT_WORKFLOW {
diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
new file mode 100644
index 000000000..2b7da3d3c
--- /dev/null
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
@@ -0,0 +1,73 @@
+nextflow_workflow {
+
+    name "Test Subworkflow FUSIONREPORT_WORKFLOW"
+    script "../main.nf"
+    workflow "FUSIONREPORT_WORKFLOW"
+
+    tag "subworkflow"
+    tag "fusionreport_workflow"
+    tag "fusionreport"
+
+    test("FUSIONREPORT_WORKFLOW - Full Test") {
+
+        setup {
+            run("FUSIONREPORT_DOWNLOAD") {
+                script "../../../../modules/local/fusionreport/download/main.nf"
+                process {
+                    """
+                    """
+                }
+            }
+        }
+
+        when {
+            workflow {
+                """
+                // Input channels
+                input[0] = Channel.of(
+                    [ [ id:'test_sample' ],
+                      file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz") ]
+                )
+
+                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
+
+                input[2] = Channel.of(
+                      [[ id:'test_sample' ],
+                        file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv") ]
+                )
+
+                input[3] =  Channel.of(
+                      [[ id:'test_sample' ],
+                        file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv") ]
+                )
+
+                input[4] =  Channel.of(
+                      [[ id:'test_sample' ],
+                        file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt") ]
+                )
+
+                """
+            }
+            params {
+                fusioninspector_only = false
+                tools_cutoff = 1
+                outdir               = "$outputDir"
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(
+                    workflow.out.fusion_list,
+                    workflow.out.fusion_list_filtered,
+                    workflow.out.report,
+                    workflow.out.csv,
+                    workflow.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    
+}

From 8e32484c11216583dfe6792db549cfdc5376c74a Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Thu, 2 Jan 2025 21:47:15 +0100
Subject: [PATCH 307/887] update snaps after merging

---
 .../local/fusioncatcher/build/environment.yml |  5 ++++
 modules/local/fusioncatcher/build/main.nf     | 10 +++++---
 .../fusioncatcher/detect/environment.yml      |  5 ++--
 modules/local/fusioncatcher/detect/main.nf    | 25 +++++++++++--------
 .../fusioncatcher/download/environment.yml    |  5 ++--
 modules/local/fusioncatcher/download/main.nf  |  8 +++---
 .../fusioncatcher_workflow/tests/main.nf.test | 15 ++++++-----
 .../tests/main.nf.test.snap                   |  2 +-
 workflows/rnafusion.nf                        |  9 ++++---
 9 files changed, 51 insertions(+), 33 deletions(-)
 create mode 100644 modules/local/fusioncatcher/build/environment.yml

diff --git a/modules/local/fusioncatcher/build/environment.yml b/modules/local/fusioncatcher/build/environment.yml
new file mode 100644
index 000000000..b5d59e181
--- /dev/null
+++ b/modules/local/fusioncatcher/build/environment.yml
@@ -0,0 +1,5 @@
+channels:
+- conda-forge
+- bioconda
+dependencies:
+- bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 4aaaf504e..3dcdd36b0 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -2,14 +2,17 @@ process FUSIONCATCHER_BUILD {
     tag "fusioncatcher_build"
     label 'process_medium'
 
-    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
+    conda "${projectDir}/environment.yml" 
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
+        'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"
 
     input:
     val genome_gencode_version
 
     output:
-    path "human_v${genome_gencode_version}"  , emit: reference
-    path "versions.yml"                      , emit: versions
+    tuple env(meta), path("human_v${genome_gencode_version}"), emit: reference
+    path "versions.yml"                                      , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -17,6 +20,7 @@ process FUSIONCATCHER_BUILD {
     script:
 
     def args = task.ext.args ?: ''
+    meta = [ id: "human_v${genome_gencode_version}" ]
     """
     fusioncatcher-build.py \\
         -g homo_sapiens \\
diff --git a/modules/local/fusioncatcher/detect/environment.yml b/modules/local/fusioncatcher/detect/environment.yml
index 312995854..b5d59e181 100644
--- a/modules/local/fusioncatcher/detect/environment.yml
+++ b/modules/local/fusioncatcher/detect/environment.yml
@@ -1,4 +1,5 @@
 channels:
-  - bioconda
+- conda-forge
+- bioconda
 dependencies:
-  - bioconda::fusioncatcher=1.33
+- bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 385172574..8e6cdcf23 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -1,35 +1,38 @@
 process FUSIONCATCHER_DETECT {
     tag "$meta.id"
     label 'process_high'
-
-    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
+    
+    conda "${projectDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
+        'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"
 
     input:
     tuple val(meta), path(fastqs, stageAs: "input/*")
     tuple val(meta2), path(reference)
 
     output:
-    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt")   , optional:true  , emit: fusions
-    tuple val(meta), path("*.fusioncatcher.summary.txt")        , optional:true  , emit: summary
-    tuple val(meta), path("*.fusioncatcher.log")                                 , emit: log
-    path "versions.yml"                                                          , emit: versions
+    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt"), emit: fusions, optional: true 
+    tuple val(meta), path("*.fusioncatcher.summary.txt")     , emit: summary, optional: true
+    tuple val(meta), path("*.fusioncatcher.log")             , emit: log
+    path "versions.yml"                                      , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def args       = task.ext.args ?: ''
+    def prefix     = task.ext.prefix ?: "${meta.id}"
     def single_end = meta.single_end ? "--single-end" : ""
     """
     fusioncatcher.py \\
         -d ${reference} \\
         -i input \\
-        -p $task.cpus \\
+        -p ${task.cpus} \\
         -o . \\
         --skip-blat \\
-        $single_end \\
-        $args
+        ${single_end} \\
+        ${args}
 
     mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
     mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
diff --git a/modules/local/fusioncatcher/download/environment.yml b/modules/local/fusioncatcher/download/environment.yml
index 312995854..b5d59e181 100644
--- a/modules/local/fusioncatcher/download/environment.yml
+++ b/modules/local/fusioncatcher/download/environment.yml
@@ -1,4 +1,5 @@
 channels:
-  - bioconda
+- conda-forge
+- bioconda
 dependencies:
-  - bioconda::fusioncatcher=1.33
+- bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 3e384faca..cbf4585c8 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -2,7 +2,10 @@ process FUSIONCATCHER_DOWNLOAD {
     tag "fusioncatcher_download"
     label 'process_medium'
 
-    container "docker.io/rannickscilifelab/fusioncatcher:1.34"
+    conda "${projectDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
+        'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"
 
     input:
     val genome_gencode_version
@@ -17,8 +20,7 @@ process FUSIONCATCHER_DOWNLOAD {
     script:
 
     def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    meta = [ id: "human_${genome_gencode_version}" ]
+    meta = [ id: "human_v${genome_gencode_version}" ]
     """
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.aa
     wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.ab
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index d0304380a..baa9eb999 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -5,23 +5,23 @@ nextflow_workflow {
     workflow "FUSIONCATCHER_WORKFLOW"
     tag "subworkflow"
     tag "fusioncatcher"
-    tag "fusioncatcher/download"
+    tag "fusioncatcher/build"
     tag "fusioncatcher/detect"
 
     // Test
     test("FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4") {
 
-        /*setup {
+        setup {
             // Download reference files for fusioncatch
-            run("FUSIONCATCHER_DOWNLOAD") {
-                script "../../../../modules/local/fusioncatcher/download/main.nf"
+            run("FUSIONCATCHER_BUILD") {
+                script "../../../../modules/local/fusioncatcher/build/main.nf"
                 process {
                 """
-                // No arguments required. Human version v102
+                input[0] = Channel.value('102')
                 """
                 }
             }
-        }*/
+        }
         
         // TODO: get smaller reference files for fusioncatcher
         when {
@@ -38,8 +38,7 @@ nextflow_workflow {
                     ] )
 
                 // ch_references
-                input[1] = Channel.fromPath("complete/with/your/local/path", checkIfExists: true)
-                    .map{ [[id: "human_v102" ], it] } //FUSIONCATCHER_DOWNLOAD.out.reference 
+                input[1] = FUSIONCATCHER_BUILD.out.reference 
 
                 // fusioncatcher (boolean)
                 input[2] = true
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
index 523916e4b..98ebe812a 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -32,6 +32,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.0"
         },
-        "timestamp": "2024-12-19T17:44:38.284199"
+        "timestamp": "2025-01-02T21:43:44.92688"
     }
 }
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2faee6af0..d2346a0b2 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -116,11 +116,14 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
 
 
-    //Run fusioncatcher
+    //R un fusioncatcher
     // TODO: check this inputs!
+    ch_fustioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref, checkIfExists: true)
+        .map{ [ [id: it.name], it] }
+
     FUSIONCATCHER_WORKFLOW (
-        ch_reads_fusioncatcher,
-        params.fusioncatcher_ref,       // channel [ meta, path       ]
+        ch_reads,
+        ch_fustioncatcher_ref,       // channel [ meta, path       ]
         params.run_fusioncatcher,
         params.all,
         params.fusioninspector_only,

From 0f9b3e78b330d724eedf15875f93839e131c1c90 Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Thu, 2 Jan 2025 21:52:42 +0100
Subject: [PATCH 308/887] remove bakcup file and update changelog

---
 CHANGELOG.md                                  |  1 +
 .../local/fusioncatcher_workflow.nf.bkp       | 34 -------------------
 2 files changed, 1 insertion(+), 34 deletions(-)
 delete mode 100644 subworkflows/local/fusioncatcher_workflow.nf.bkp

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e61cc28a6..0f550365a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local module: `STARFUSION_DETECT`. [#586](https://github.com/nf-core/rnafusion/pull/586)
 - Added a new module `CTATSPLICING_STARTOCANCERINTRONS` and a new parameter `--ctatsplicing`. This options creates reports on cancer splicing abberations and requires one or both of `--arriba` and `--starfusion` to be given. [#587](https://github.com/nf-core/rnafusion/pull/587)
 - Add parameter `--references_only` when no data should be analysed, but only the references should be built [#505](https://github.com/nf-core/rnafusion/pull/505)
+- Add nf-test to local subworkflow: `FUSIONCATCHER_WORKFLOW` [#591](https://github.com/nf-core/rnafusion/pull/591)
 - Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)
 
 ### Changed
diff --git a/subworkflows/local/fusioncatcher_workflow.nf.bkp b/subworkflows/local/fusioncatcher_workflow.nf.bkp
deleted file mode 100644
index 97ed55de8..000000000
--- a/subworkflows/local/fusioncatcher_workflow.nf.bkp
+++ /dev/null
@@ -1,34 +0,0 @@
-include { FUSIONCATCHER }                       from '../../modules/local/fusioncatcher/detect/main'
-
-
-workflow FUSIONCATCHER_WORKFLOW {
-    take:
-        reads
-
-    main:
-        ch_versions = Channel.empty()
-        ch_dummy_file = file("$baseDir/assets/dummy_file_fusioncatcher.txt", checkIfExists: true)
-
-        if ((params.fusioncatcher || params.all) && !params.fusioninspector_only) {
-            if (params.fusioncatcher_fusions){
-                ch_fusioncatcher_fusions = reads.combine(Channel.value(file(params.fusioncatcher_fusions, checkIfExists:true)))
-                                            .map { it -> [ it[0], it[1] ] }
-            } else {
-                FUSIONCATCHER (
-                    reads,
-                    params.fusioncatcher_ref
-                )
-                ch_fusioncatcher_fusions = FUSIONCATCHER.out.fusions
-                ch_versions = ch_versions.mix(FUSIONCATCHER.out.versions)
-            }
-        }
-        else {
-            ch_fusioncatcher_fusions = reads.combine(Channel.value(file(ch_dummy_file, checkIfExists:true)))
-                                        .map { it -> [ it[0], it[1] ] }
-        }
-
-    emit:
-        fusions  = ch_fusioncatcher_fusions
-        versions = ch_versions
-    }
-

From 78a3230d9c89a6a60f8f70802d6ef95d5f270398 Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Fri, 3 Jan 2025 19:40:39 +0100
Subject: [PATCH 309/887] fix bug with fusioncatcher ref

---
 modules/local/fusioncatcher/build/main.nf     |  4 +--
 modules/local/fusioncatcher/detect/main.nf    |  4 +--
 modules/local/fusioncatcher/download/main.nf  |  4 +--
 .../fusioncatcher_workflow/tests/main.nf.test |  2 +-
 .../tests/main.nf.test.snap                   |  8 ++---
 tests/test_stub.nf.test.snap                  | 30 +++++++++----------
 workflows/rnafusion.nf                        |  8 ++---
 7 files changed, 28 insertions(+), 32 deletions(-)

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 3dcdd36b0..258fbca23 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -29,7 +29,7 @@ process FUSIONCATCHER_BUILD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
     END_VERSIONS
     """
 
@@ -39,7 +39,7 @@ process FUSIONCATCHER_BUILD {
     touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
     END_VERSIONS
     """
 }
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index 8e6cdcf23..f86702000 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -40,7 +40,7 @@ process FUSIONCATCHER_DETECT {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: \$(echo \$(fusioncatcher.py --version 2>&1)| sed 's/fusioncatcher.py //')
+        fusioncatcher: "\$(fusioncatcher.py --version 2>&1 | awk '{print \$2}')"
     END_VERSIONS
     """
 
@@ -53,7 +53,7 @@ process FUSIONCATCHER_DETECT {
     touch ${prefix}.fusioncatcher.log
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: \$(echo \$(fusioncatcher.py --version 2>&1)| sed 's/fusioncatcher.py //')
+        fusioncatcher: "\$(fusioncatcher.py --version 2>&1 | awk '{print \$2}')"
     END_VERSIONS
     """
 }
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index cbf4585c8..af585d62c 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -31,7 +31,7 @@ process FUSIONCATCHER_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
     END_VERSIONS
     """
 
@@ -41,7 +41,7 @@ process FUSIONCATCHER_DOWNLOAD {
     touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: \$(echo \$(fusioncatcher --version 2>&1))
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
     END_VERSIONS
     """
 }
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index baa9eb999..59c60595b 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -17,7 +17,7 @@ nextflow_workflow {
                 script "../../../../modules/local/fusioncatcher/build/main.nf"
                 process {
                 """
-                input[0] = Channel.value('102')
+                input[0] = Channel.value('46')
                 """
                 }
             }
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
index 98ebe812a..47ffc5af1 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -12,7 +12,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,6462518e02774293fa221cead8373305"
+                    "versions.yml:md5,05bd93a243728a293211ce52e5f97282"
                 ],
                 "fusions": [
                     [
@@ -24,14 +24,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,6462518e02774293fa221cead8373305"
+                    "versions.yml:md5,05bd93a243728a293211ce52e5f97282"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.0"
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2025-01-02T21:43:44.92688"
+        "timestamp": "2025-01-03T19:29:54.767628"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 0ac620e73..93354967e 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -15,11 +15,11 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER": {
-                    "fusioncatcher": 1.35
-                },
                 "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "fusioncatcher.py 1.35"
+                    "fusioncatcher": "1.33"
+                },
+                "FUSIONCATCHER_DETECT": {
+                    "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
@@ -354,10 +354,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-20T14:00:19.311265"
+        "timestamp": "2025-01-03T14:17:57.064279"
     },
     "stub test with fastp trim": {
         "content": [
@@ -381,11 +381,11 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER": {
-                    "fusioncatcher": 1.35
-                },
                 "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "fusioncatcher.py 1.35"
+                    "fusioncatcher": "1.33"
+                },
+                "FUSIONCATCHER_DETECT": {
+                    "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
@@ -729,9 +729,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-20T13:57:10.179877"
+        "timestamp": "2025-01-03T14:16:13.691547"
     }
-}
+}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index d2346a0b2..d8e717ecc 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -116,14 +116,10 @@ workflow RNAFUSION {
     ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
 
 
-    //R un fusioncatcher
-    // TODO: check this inputs!
-    ch_fustioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref, checkIfExists: true)
-        .map{ [ [id: it.name], it] }
-
+    //Run fusioncatcher
     FUSIONCATCHER_WORKFLOW (
         ch_reads,
-        ch_fustioncatcher_ref,       // channel [ meta, path       ]
+        BUILD_REFERENCES.out.ch_fusioncatcher_ref,       // channel [ meta, path       ]
         params.run_fusioncatcher,
         params.all,
         params.fusioninspector_only,

From 8cac4707bb4c9fb67fb6b58072363fee421c0dfd Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Fri, 3 Jan 2025 19:45:23 +0100
Subject: [PATCH 310/887] pre-commit changes

---
 modules/local/fusioncatcher/build/main.nf                    | 2 +-
 modules/local/fusioncatcher/detect/main.nf                   | 4 ++--
 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test | 4 ++--
 3 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
index 258fbca23..db1c72107 100644
--- a/modules/local/fusioncatcher/build/main.nf
+++ b/modules/local/fusioncatcher/build/main.nf
@@ -2,7 +2,7 @@ process FUSIONCATCHER_BUILD {
     tag "fusioncatcher_build"
     label 'process_medium'
 
-    conda "${projectDir}/environment.yml" 
+    conda "${projectDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
         'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
index f86702000..7980439f8 100644
--- a/modules/local/fusioncatcher/detect/main.nf
+++ b/modules/local/fusioncatcher/detect/main.nf
@@ -1,7 +1,7 @@
 process FUSIONCATCHER_DETECT {
     tag "$meta.id"
     label 'process_high'
-    
+
     conda "${projectDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
@@ -12,7 +12,7 @@ process FUSIONCATCHER_DETECT {
     tuple val(meta2), path(reference)
 
     output:
-    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt"), emit: fusions, optional: true 
+    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt"), emit: fusions, optional: true
     tuple val(meta), path("*.fusioncatcher.summary.txt")     , emit: summary, optional: true
     tuple val(meta), path("*.fusioncatcher.log")             , emit: log
     path "versions.yml"                                      , emit: versions
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index 59c60595b..ef6cecc7d 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -22,7 +22,7 @@ nextflow_workflow {
                 }
             }
         }
-        
+
         // TODO: get smaller reference files for fusioncatcher
         when {
             workflow {
@@ -38,7 +38,7 @@ nextflow_workflow {
                     ] )
 
                 // ch_references
-                input[1] = FUSIONCATCHER_BUILD.out.reference 
+                input[1] = FUSIONCATCHER_BUILD.out.reference
 
                 // fusioncatcher (boolean)
                 input[2] = true

From 557a0ed45da78438808da1fa875c649520f59390 Mon Sep 17 00:00:00 2001
From: Alan M <alanmmobbs@gmail.com>
Date: Fri, 3 Jan 2025 20:04:27 +0100
Subject: [PATCH 311/887] prettier changes

---
 modules/local/fusioncatcher/build/environment.yml    | 6 +++---
 modules/local/fusioncatcher/detect/environment.yml   | 6 +++---
 modules/local/fusioncatcher/download/environment.yml | 6 +++---
 3 files changed, 9 insertions(+), 9 deletions(-)

diff --git a/modules/local/fusioncatcher/build/environment.yml b/modules/local/fusioncatcher/build/environment.yml
index b5d59e181..8e83de36b 100644
--- a/modules/local/fusioncatcher/build/environment.yml
+++ b/modules/local/fusioncatcher/build/environment.yml
@@ -1,5 +1,5 @@
 channels:
-- conda-forge
-- bioconda
+  - conda-forge
+  - bioconda
 dependencies:
-- bioconda::fusioncatcher=1.33
+  - bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/detect/environment.yml b/modules/local/fusioncatcher/detect/environment.yml
index b5d59e181..8e83de36b 100644
--- a/modules/local/fusioncatcher/detect/environment.yml
+++ b/modules/local/fusioncatcher/detect/environment.yml
@@ -1,5 +1,5 @@
 channels:
-- conda-forge
-- bioconda
+  - conda-forge
+  - bioconda
 dependencies:
-- bioconda::fusioncatcher=1.33
+  - bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/download/environment.yml b/modules/local/fusioncatcher/download/environment.yml
index b5d59e181..8e83de36b 100644
--- a/modules/local/fusioncatcher/download/environment.yml
+++ b/modules/local/fusioncatcher/download/environment.yml
@@ -1,5 +1,5 @@
 channels:
-- conda-forge
-- bioconda
+  - conda-forge
+  - bioconda
 dependencies:
-- bioconda::fusioncatcher=1.33
+  - bioconda::fusioncatcher=1.33

From a04128cd7d036fe58067e50b567abe7b4bbec69c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 7 Jan 2025 10:37:08 +0100
Subject: [PATCH 312/887] fix some merge conflicts

---
 main.nf                                       |  6 +--
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  3 +-
 tests/test_stub.nf.test.snap                  | 38 +++----------------
 3 files changed, 10 insertions(+), 37 deletions(-)

diff --git a/main.nf b/main.nf
index ced04ca91..efe3437ac 100644
--- a/main.nf
+++ b/main.nf
@@ -56,7 +56,8 @@ workflow NFCORE_RNAFUSION {
     } else {
         RNAFUSION(samplesheet)
     }
-
+    emit:
+    RNAFUSION.out.multiqc_report
 }
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -73,10 +74,8 @@ workflow {
     PIPELINE_INITIALISATION (
         params.version,
         params.validate_params,
-        params.monochrome_logs,
         args,
         params.outdir,
-        params.input
     )
 
     //
@@ -96,6 +95,7 @@ workflow {
         params.outdir,
         params.monochrome_logs,
         params.hook_url,
+        NFCORE_RNAFUSION.out.multiqc_report
     )
 }
 
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 8b08603ce..9cff37c6a 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -30,10 +30,8 @@ workflow PIPELINE_INITIALISATION {
     take:
     version           // boolean: Display version and exit
     validate_params   // boolean: Boolean whether to validate parameters against the schema at runtime
-    monochrome_logs   // boolean: Do not use coloured log outputs
     nextflow_cli_args //   array: List of positional nextflow CLI args
     outdir            //  string: The output directory where the results will be saved
-    input             //  string: Path to input samplesheet
 
     main:
 
@@ -114,6 +112,7 @@ workflow PIPELINE_COMPLETION {
     outdir          //    path: Path to output directory where results will be published
     monochrome_logs // boolean: Disable ANSI colour codes in log output
     hook_url        //  string: hook URL for notifications
+    multiqc_report
 
     main:
     summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json")
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 3b83c2400..117067622 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -2,17 +2,7 @@
     "stub test no fastp trim": {
         "content": [
             4,
-            {
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            null,
             [
                 "fastqc",
                 "fastqc/test.html",
@@ -30,7 +20,7 @@
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
                 "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -41,28 +31,12 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T19:27:00.717876194"
+        "timestamp": "2024-12-20T15:40:14.297437"
     },
     "stub test with fastp trim": {
         "content": [
             6,
-            {
-                "FASTP": {
-                    "fastp": "0.23.4"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FASTQC_FOR_FASTP": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            null,
             [
                 "fastp",
                 "fastp/test.fastp.html",
@@ -89,7 +63,7 @@
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
                 "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -100,6 +74,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2024-12-03T19:26:43.375635514"
+        "timestamp": "2024-12-20T15:39:20.981018"
     }
 }
\ No newline at end of file

From c5c45c0e2d628607d9dc5e34df7ab017ae70e89e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 7 Jan 2025 13:20:24 +0100
Subject: [PATCH 313/887] update softwareversion file

---
 .../utils_nfcore_rnafusion_pipeline/main.nf   |   2 +-
 tests/test_cosmic.nf.test                     |   6 +-
 tests/test_cosmic.nf.test.snap                |  10 +-
 tests/test_stub.nf.test                       |   4 +-
 tests/test_stub.nf.test.snap                  | 210 +++++++++++++++++-
 5 files changed, 217 insertions(+), 15 deletions(-)

diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index c57ce9f3b..7e0362286 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -130,7 +130,7 @@ workflow PIPELINE_COMPLETION {
                 plaintext_email,
                 outdir,
                 monochrome_logs,
-                multiqc_reports.getVal(),
+                multiqc_reports.getVal()
             )
         }
 
diff --git a/tests/test_cosmic.nf.test b/tests/test_cosmic.nf.test
index 9a56e76e5..b4f2a3639 100644
--- a/tests/test_cosmic.nf.test
+++ b/tests/test_cosmic.nf.test
@@ -28,7 +28,7 @@ nextflow_pipeline {
                     // Number of successful tasks
                     workflow.trace.succeeded().size(),
                     // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
@@ -58,7 +58,7 @@ nextflow_pipeline {
                     // Number of successful tasks
                     workflow.trace.succeeded().size(),
                     // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
@@ -89,7 +89,7 @@ nextflow_pipeline {
                     // Number of successful tasks
                     workflow.trace.succeeded().size(),
                     // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
diff --git a/tests/test_cosmic.nf.test.snap b/tests/test_cosmic.nf.test.snap
index b8e6d4a5f..c920e0321 100644
--- a/tests/test_cosmic.nf.test.snap
+++ b/tests/test_cosmic.nf.test.snap
@@ -9,10 +9,10 @@
             },
             [
                 "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml"
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"
             ],
             [
-                
+
             ]
         ],
         "meta": {
@@ -29,7 +29,7 @@
                 "pipeline_info"
             ],
             [
-                
+
             ]
         ],
         "meta": {
@@ -46,7 +46,7 @@
                 "pipeline_info"
             ],
             [
-                
+
             ]
         ],
         "meta": {
@@ -55,4 +55,4 @@
         },
         "timestamp": "2024-12-10T15:09:29.277399"
     }
-}
\ No newline at end of file
+}
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index b0601a9f7..065723e2f 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -30,7 +30,7 @@ nextflow_pipeline {
                     // Number of successful tasks
                     workflow.trace.succeeded().size(),
                     // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
@@ -61,7 +61,7 @@ nextflow_pipeline {
                     // Number of successful tasks
                     workflow.trace.succeeded().size(),
                     // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index c535947e3..91d847eb8 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -2,7 +2,105 @@
     "stub test no fastp trim": {
         "content": [
             31,
-            null,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "CTATSPLICING_STARTOCANCERINTRONS": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.35
+                },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "fusioncatcher.py 1.35"
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": null
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
@@ -259,12 +357,116 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2025-01-07T10:53:09.572584"
+        "timestamp": "2025-01-07T13:16:02.754052"
     },
     "stub test with fastp trim": {
         "content": [
             33,
-            null,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "CTATSPLICING_STARTOCANCERINTRONS": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER": {
+                    "fusioncatcher": 1.35
+                },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "fusioncatcher.py 1.35"
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": null
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_FOR_ARRIBA": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": null,
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
@@ -530,6 +732,6 @@
             "nf-test": "0.9.0",
             "nextflow": "24.10.2"
         },
-        "timestamp": "2025-01-07T10:50:24.959026"
+        "timestamp": "2025-01-07T13:13:38.690939"
     }
 }
\ No newline at end of file

From 5a019d14600ba5361ca13649d040db173272c492 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 7 Jan 2025 13:31:19 +0100
Subject: [PATCH 314/887] update version

---
 tests/test_stub.nf.test.snap | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 91d847eb8..92a7c0eea 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -77,17 +77,17 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -443,17 +443,17 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_FOR_ARRIBA": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
-                    "star": null,
+                    "star": "2.7.11b",
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
@@ -734,4 +734,4 @@
         },
         "timestamp": "2025-01-07T13:13:38.690939"
     }
-}
\ No newline at end of file
+}

From 1516bcadd2bc907a43d345aff98207a532ab6d91 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 7 Jan 2025 13:35:20 +0000
Subject: [PATCH 315/887] feat: update `fusion-report` to 3.1.1

---
 modules/local/fusionreport/detect/environment.yml   | 6 ++++++
 modules/local/fusionreport/detect/main.nf           | 5 ++++-
 modules/local/fusionreport/download/environment.yml | 6 ++++++
 modules/local/fusionreport/download/main.nf         | 6 ++++--
 4 files changed, 20 insertions(+), 3 deletions(-)
 create mode 100644 modules/local/fusionreport/detect/environment.yml
 create mode 100644 modules/local/fusionreport/download/environment.yml

diff --git a/modules/local/fusionreport/detect/environment.yml b/modules/local/fusionreport/detect/environment.yml
new file mode 100644
index 000000000..0d260fdb8
--- /dev/null
+++ b/modules/local/fusionreport/detect/environment.yml
@@ -0,0 +1,6 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::fusion-report=3.1.1
+  - conda-forge::openpyxl=3.1.5
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 56a29ab4c..3670eec0b 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -2,7 +2,10 @@ process FUSIONREPORT {
     tag "$meta.id"
     label 'process_medium'
 
-    container "docker.io/clinicalgenomics/fusion-report:3.1.0"
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d9d1075dc45da6b08ec99c6e8bcc83e0ab71a674e7efdc7a36e459539793fcf9/data' :
+        'community.wave.seqera.io/library/fusion-report_openpyxl:6748677442b83a9a'}"
 
 
     input:
diff --git a/modules/local/fusionreport/download/environment.yml b/modules/local/fusionreport/download/environment.yml
new file mode 100644
index 000000000..0d260fdb8
--- /dev/null
+++ b/modules/local/fusionreport/download/environment.yml
@@ -0,0 +1,6 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::fusion-report=3.1.1
+  - conda-forge::openpyxl=3.1.5
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 89f631db4..ec62c75eb 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -2,8 +2,10 @@ process FUSIONREPORT_DOWNLOAD {
     tag 'fusionreport'
     label 'process_medium'
 
-    conda "bioconda::star=2.7.9a"
-    container "docker.io/clinicalgenomics/fusion-report:3.1.0"
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d9d1075dc45da6b08ec99c6e8bcc83e0ab71a674e7efdc7a36e459539793fcf9/data' :
+        'community.wave.seqera.io/library/fusion-report_openpyxl:6748677442b83a9a'}"
 
     output:
     tuple val(meta), path("fusion_report_db"), emit: fusionreport_ref

From f40e9f5f8532e06195654a3eb9e3f90a0ccba959 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 7 Jan 2025 13:37:13 +0000
Subject: [PATCH 316/887] test: add required params

---
 .../fusionreport_workflow/tests/main.nf.test  | 14 ++++--
 .../tests/main.nf.test.snap                   | 46 +++++++++++++++++++
 2 files changed, 55 insertions(+), 5 deletions(-)
 create mode 100644 subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap

diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
index 2b7da3d3c..61aaf023d 100644
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
@@ -26,23 +26,23 @@ nextflow_workflow {
                 // Input channels
                 input[0] = Channel.of(
                     [ [ id:'test_sample' ],
-                      file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz") ]
+                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz") ]
                 )
 
                 input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
 
                 input[2] = Channel.of(
-                      [[ id:'test_sample' ],
+                    [[ id:'test_sample' ],
                         file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv") ]
                 )
 
                 input[3] =  Channel.of(
-                      [[ id:'test_sample' ],
+                    [[ id:'test_sample' ],
                         file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv") ]
                 )
 
                 input[4] =  Channel.of(
-                      [[ id:'test_sample' ],
+                    [[ id:'test_sample' ],
                         file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt") ]
                 )
 
@@ -51,6 +51,10 @@ nextflow_workflow {
             params {
                 fusioninspector_only = false
                 tools_cutoff = 1
+                arriba          = true
+                starfusion      = true
+                fusioncatcher   = true
+                no_cosmic       = true
                 outdir               = "$outputDir"
             }
         }
@@ -69,5 +73,5 @@ nextflow_workflow {
         }
     }
 
-    
+
 }
diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap b/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..2c384e5a2
--- /dev/null
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
@@ -0,0 +1,46 @@
+{
+    "FUSIONREPORT_WORKFLOW - Full Test": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "test_sample.fusionreport.tsv:md5,3593b7021f26cc5427fdc96f0d1c72f0"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "test_sample.fusionreport_filtered.tsv:md5,3593b7021f26cc5427fdc96f0d1c72f0"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "test_sample_fusionreport_index.html:md5,3513bcaa58446399c0957db69402d3bd"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test_sample"
+                    },
+                    "test_sample.fusions.csv:md5,49f378c2112d7e0b3b17d9095c79e6bd"
+                ]
+            ],
+            [
+                "versions.yml:md5,90749dbf8e3e7b259c935eabb8c6ce1e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-01-07T13:31:41.215356596"
+    }
+}
\ No newline at end of file

From f976de19694abce63c010c2ce83a80c04d0b0f3f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 9 Jan 2025 12:42:18 +0000
Subject: [PATCH 317/887] test: adapt the test to new template

---
 tests/test_build.nf.test | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 1dbda8336..f6665777b 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -27,7 +27,7 @@ nextflow_pipeline {
                     // Number of successful tasks
                     workflow.trace.succeeded().size(),
                     // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"),
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
                     // All stable path name, with a relative path
                     stable_name,
                     // All files with stable contents

From 2a2d9ca032786848266ab2c612640e46a16b71c2 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 9 Jan 2025 13:40:10 +0000
Subject: [PATCH 318/887] test: try fix on template merge issue

---
 tests/test_build.nf.test.snap | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index c8aefa908..f2e1bc38f 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -52,7 +52,7 @@
                 "get/rrna.bed",
                 "get/rrna.gtf",
                 "pipeline_info",
-                "pipeline_info/nf_core_pipeline_software_mqc_versions.yml",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
                 "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
@@ -197,7 +197,7 @@
                 "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
-                "hgnc_complete_set.txt:md5,a563a2f8432ec0ab7d3dc74d769102b8",
+                "hgnc_complete_set.txt:md5,ba66fe5d27ce643b5636a2a85b1b32cb",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
                 "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
@@ -284,4 +284,4 @@
         },
         "timestamp": "2024-12-30T13:35:33.573499693"
     }
-}
\ No newline at end of file
+}

From b7c928b02b2e532d4128e5edd86a3b2937cf2242 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 14 Jan 2025 13:10:10 +0000
Subject: [PATCH 319/887] test: update tests and snapshot

---
 tests/.nftignore              | 1 +
 tests/test_build.nf.test.snap | 9 ++++-----
 2 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/tests/.nftignore b/tests/.nftignore
index 7b5b68596..07c82b438 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -3,6 +3,7 @@ references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
 references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
 references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa
 references/hgnc/HGNC-DB-timestamp.txt
+references/hgnc/hgnc_complete_set.txt
 references/fusion_report_db/DB-timestamp.txt
 references/salmon/salmon/ctable.bin
 references/salmon/salmon/pos.bin
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index f2e1bc38f..d1d798fc7 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -191,13 +191,12 @@
                 "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
                 "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a",
-                "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a",
+                "fusiongdb2.db:md5,715b52d8fa4270c510240c48fb88e9c7",
+                "mitelman.db:md5,51e307dbf4c35c425a3473baa44939c0",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
-                "hgnc_complete_set.txt:md5,ba66fe5d27ce643b5636a2a85b1b32cb",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
                 "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
@@ -282,6 +281,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-30T13:35:33.573499693"
+        "timestamp": "2025-01-14T13:08:24.564679638"
     }
-}
+}
\ No newline at end of file

From 675c0a3b835c7b434a6faa76187186191ff5f3b5 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 14 Jan 2025 14:12:05 +0000
Subject: [PATCH 320/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 010d16194..3738dca25 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -28,6 +28,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `FUSIONCATCHER_WORKFLOW` [#591](https://github.com/nf-core/rnafusion/pull/591)
 - Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)
 - Add nf-test to local module: `FUSIONINSPECTOR`. [#601](https://github.com/nf-core/rnafusion/pull/601)
+- Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
 
 ### Changed
 

From 7bfea342bb32e7cf713225f3af45e65356478c4e Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Mon, 20 Jan 2025 14:37:10 +0000
Subject: [PATCH 321/887] Template update for nf-core/tools version 3.1.2

---
 .github/workflows/ci.yml                |  2 +
 .github/workflows/download_pipeline.yml | 28 +++++----
 .nf-core.yml                            |  2 +-
 CITATIONS.md                            |  4 +-
 README.md                               | 10 ++-
 conf/test.config                        |  4 +-
 nextflow.config                         |  2 +-
 ro-crate-metadata.json                  | 81 +++++++++----------------
 8 files changed, 61 insertions(+), 72 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 68412b2ce..78fb3b574 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -46,6 +46,8 @@ jobs:
     steps:
       - name: Check out pipeline code
         uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
+        with:
+          fetch-depth: 0
 
       - name: Set up Nextflow
         uses: nf-core/setup-nextflow@v2
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 13b51e2c3..ab06316ea 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -34,6 +34,17 @@ jobs:
       REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }}
       REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }}
       REPO_BRANCH: ${{ steps.get_repo_properties.outputs.REPO_BRANCH }}
+    steps:
+      - name: Get the repository name and current branch
+        id: get_repo_properties
+        run: |
+          echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT"
+          echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT"
+          echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT"
+
+  download:
+    runs-on: ubuntu-latest
+    needs: configure
     steps:
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
@@ -56,21 +67,10 @@ jobs:
           python -m pip install --upgrade pip
           pip install git+https://github.com/nf-core/tools.git@dev
 
-      - name: Get the repository name and current branch set as environment variable
-        id: get_repo_properties
-        run: |
-          echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT"
-          echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT"
-          echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT"
-
       - name: Make a cache directory for the container images
         run: |
           mkdir -p ./singularity_container_images
 
-  download:
-    runs-on: ubuntu-latest
-    needs: configure
-    steps:
       - name: Download the pipeline
         env:
           NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
@@ -87,6 +87,9 @@ jobs:
       - name: Inspect download
         run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}
 
+      - name: Inspect container images
+        run: tree ./singularity_container_images | tee ./container_initial
+
       - name: Count the downloaded number of container images
         id: count_initial
         run: |
@@ -123,7 +126,8 @@ jobs:
             final_count=${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}
             difference=$((final_count - initial_count))
             echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!"
-            tree ./singularity_container_images
+            tree ./singularity_container_images > ./container_afterwards
+            diff ./container_initial ./container_afterwards
             exit 1
           else
             echo "The pipeline can be downloaded successfully!"
diff --git a/.nf-core.yml b/.nf-core.yml
index 148865a49..dbf96efef 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,7 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
-nf_core_version: 3.1.1
+nf_core_version: 3.1.2
 repository_type: pipeline
 template:
   author: Martin Proks, Annick Renevey
diff --git a/CITATIONS.md b/CITATIONS.md
index 93ddd1a75..0fc81801b 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -12,7 +12,9 @@
 
 - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
 
-> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
+> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].
+
+- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
 
 > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
 
diff --git a/README.md b/README.md
index 12b71f87f..f40e75a67 100644
--- a/README.md
+++ b/README.md
@@ -3,7 +3,9 @@
     <source media="(prefers-color-scheme: dark)" srcset="docs/images/nf-core-rnafusion_logo_dark.png">
     <img alt="nf-core/rnafusion" src="docs/images/nf-core-rnafusion_logo_light.png">
   </picture>
-</h1>[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)
+</h1>
+
+[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
@@ -32,7 +34,7 @@
 ## Usage
 
 > [!NOTE]
-> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
+> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
 
 <!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.
      Explain what rows and columns represent. For instance (please edit as appropriate):
@@ -89,7 +91,9 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
 ## Citations
 
 <!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
-<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) --><!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
+<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->
+
+<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->
 
 An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.
 
diff --git a/conf/test.config b/conf/test.config
index 955708618..56ff5304f 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -25,8 +25,6 @@ params {
     // Input data
     // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
     // TODO nf-core: Give any required params for the test so that command line flags are not needed
-    input  = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'
-
-    // Genome references
+    input  = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'// Genome references
     genome = 'R64-1-1'
 }
diff --git a/nextflow.config b/nextflow.config
index bf863573a..1057767d5 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -257,7 +257,7 @@ manifest {
 
 // Nextflow plugins
 plugins {
-    id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+    id 'nf-schema@2.3.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 
 validation {
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 145ad4d00..1314af32a 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,8 +22,8 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
-            "datePublished": "2024-12-20T13:11:36+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) --><!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "datePublished": "2025-01-20T14:36:55+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#f907a90b-3444-4392-bd9f-cad7780c2dc3"
+                    "@id": "#f6260e6b-9f65-45aa-9ada-0e886fa2fb70"
                 }
             ],
             "name": "nf-core/rnafusion"
@@ -121,61 +121,40 @@
         },
         {
             "@id": "main.nf",
-            "@type": [
-                "File",
-                "SoftwareSourceCode",
-                "ComputationalWorkflow"
-            ],
+            "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
             "creator": [
                 {
-                    "@id": "https://orcid.org/0000-0002-8178-3128"
+                    "@id": "#phil.ewels@scilifelab.se"
                 },
                 {
-                    "@id": "#max.u.garcia@gmail.com"
+                    "@id": "https://orcid.org/0000-0001-9017-591X"
                 },
                 {
-                    "@id": "#phil.ewels@scilifelab.se"
+                    "@id": "https://orcid.org/0000-0002-8178-3128"
                 },
                 {
-                    "@id": "https://orcid.org/0000-0001-9017-591X"
+                    "@id": "#max.u.garcia@gmail.com"
                 }
             ],
             "dateCreated": "",
-            "dateModified": "2024-12-20T13:11:36Z",
+            "dateModified": "2025-01-20T14:36:55Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
-            "keywords": [
-                "nf-core",
-                "nextflow",
-                "fusion",
-                "fusion-genes",
-                "gene-fusion",
-                "rna",
-                "rna-seq"
-            ],
-            "license": [
-                "MIT"
-            ],
+            "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
+            "license": ["MIT"],
             "maintainer": [
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
                 }
             ],
-            "name": [
-                "nf-core/rnafusion"
-            ],
+            "name": ["nf-core/rnafusion"],
             "programmingLanguage": {
                 "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow"
             },
             "sdPublisher": {
                 "@id": "https://nf-co.re/"
             },
-            "url": [
-                "https://github.com/nf-core/rnafusion",
-                "https://nf-co.re/rnafusion/dev/"
-            ],
-            "version": [
-                "4.0.0dev"
-            ]
+            "url": ["https://github.com/nf-core/rnafusion", "https://nf-co.re/rnafusion/dev/"],
+            "version": ["4.0.0dev"]
         },
         {
             "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow",
@@ -190,11 +169,11 @@
             "version": "!>=24.04.2"
         },
         {
-            "@id": "#f907a90b-3444-4392-bd9f-cad7780c2dc3",
+            "@id": "#f6260e6b-9f65-45aa-9ada-0e886fa2fb70",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#7a00a302-505f-4b06-bd1c-5a4e1fa19bdd"
+                    "@id": "#c5ee26a1-ec22-464a-b17b-3a0c5dcf6e02"
                 }
             ],
             "mainEntity": {
@@ -203,7 +182,7 @@
             "name": "Test suite for nf-core/rnafusion"
         },
         {
-            "@id": "#7a00a302-505f-4b06-bd1c-5a4e1fa19bdd",
+            "@id": "#c5ee26a1-ec22-464a-b17b-3a0c5dcf6e02",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/rnafusion",
             "resource": "repos/nf-core/rnafusion/actions/workflows/ci.yml",
@@ -331,18 +310,6 @@
             "name": "nf-core",
             "url": "https://nf-co.re/"
         },
-        {
-            "@id": "https://orcid.org/0000-0002-8178-3128",
-            "@type": "Person",
-            "email": "mproksik@gmail.com",
-            "name": "Martin Proks"
-        },
-        {
-            "@id": "#max.u.garcia@gmail.com",
-            "@type": "Person",
-            "email": "max.u.garcia@gmail.com",
-            "name": "Maxime Garcia"
-        },
         {
             "@id": "#phil.ewels@scilifelab.se",
             "@type": "Person",
@@ -354,6 +321,18 @@
             "@type": "Person",
             "email": "rickard.hammaren@scilifelab.se",
             "name": "Rickard Hammar\u00e9n"
+        },
+        {
+            "@id": "https://orcid.org/0000-0002-8178-3128",
+            "@type": "Person",
+            "email": "mproksik@gmail.com",
+            "name": "Martin Proks"
+        },
+        {
+            "@id": "#max.u.garcia@gmail.com",
+            "@type": "Person",
+            "email": "max.u.garcia@gmail.com",
+            "name": "Maxime Garcia"
         }
     ]
-}
\ No newline at end of file
+}

From a31752950e168b58dd5bdb9ac243eef11866e892 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 21 Jan 2025 14:55:01 +0100
Subject: [PATCH 322/887] add cancer splicing lib to starfusion ref

---
 conf/modules.config                           | 15 ++++++-
 .../local/ctatsplicing/prepgenomelib/main.nf  | 45 +++++++++++++++++++
 nextflow.config                               |  1 -
 subworkflows/local/build_references.nf        | 11 ++++-
 4 files changed, 69 insertions(+), 3 deletions(-)
 create mode 100644 modules/local/ctatsplicing/prepgenomelib/main.nf

diff --git a/conf/modules.config b/conf/modules.config
index 967e820a7..c66cafadb 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -360,7 +360,20 @@ process {
         ]
     }
 
-    withName: 'NFCORE_RNAFUSION:BUILD_REFERENCES:STARFUSION_BUILD' {
+    withName: 'STARFUSION_BUILD' {
+        cpus   = { 24    * task.attempt }
+        memory = { 100.GB * task.attempt }
+        time   = { 2.d  * task.attempt }
+        publishDir = [
+            enabled: !params.ctatsplicing && !params.all,
+            path: { "${params.genomes_base}/starfusion" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+        ext.args = "--max_readlength ${params.read_length} --human_gencode_filter"
+    }
+
+    withName: 'CTATSPLICING_PREPGENOMELIB' {
         cpus   = { 24    * task.attempt }
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }
diff --git a/modules/local/ctatsplicing/prepgenomelib/main.nf b/modules/local/ctatsplicing/prepgenomelib/main.nf
new file mode 100644
index 000000000..fd7feebc4
--- /dev/null
+++ b/modules/local/ctatsplicing/prepgenomelib/main.nf
@@ -0,0 +1,45 @@
+process CTATSPLICING_PREPGENOMELIB {
+    tag "$meta.id"
+    label 'process_single'
+
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://data.broadinstitute.org/Trinity/CTAT_SINGULARITY/CTAT-SPLICING/ctat_splicing.v0.0.2.simg' :
+        'docker.io/trinityctat/ctat_splicing:0.0.2' }"
+
+    input:
+    tuple val(meta), path(genome_lib)
+
+    output:
+    tuple val(meta), path(genome_lib, includeInputs:true), emit: reference
+    path "versions.yml"                                  , emit: versions
+
+    script:
+    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz
+
+    /usr/local/src/CTAT-SPLICING/prep_genome_lib/ctat-splicing-lib-integration.py \\
+        --cancer_introns_tsv cancer_introns.*.tsv.gz \\
+        --genome_lib_dir $genome_lib
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ctat-splicing: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    touch $genome_lib/refGene.bed
+    touch $genome_lib/refGene.sort.bed.gz
+    touch $genome_lib/refGene.sort.bed.gz.tbi
+    mkdir $genome_lib/cancer_splicing_lib
+    touch $genome_lib/cancer_splicing_lib/cancer_splicing.idx
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ctat-splicing: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/nextflow.config b/nextflow.config
index 1bd38be68..d83ceecda 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -91,7 +91,6 @@ params {
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
 
-
     // Internal file presence checks
     salmon_index_stub_check       = "${params.genomes_base}/salmon/salmon/complete_ref_lens.bin"
     starindex_ref_stub_check      = "${params.genomes_base}/star/star/Genome"
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 56fd8eb24..c7cb3c31b 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -11,6 +11,7 @@ include { HGNC_DOWNLOAD }                   from '../../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../../modules/local/starfusion/build/main'
 include { GTF_TO_REFFLAT }                  from '../../modules/local/uscs/custom_gtftogenepred/main'
 include { GET_RRNA_TRANSCRIPTS }            from '../../modules/local/get_rrna_transcript/main'
+include { CTATSPLICING_PREPGENOMELIB }      from '../../modules/local/ctatsplicing/prepgenomelib/main.nf'
 
 /*
 ========================================================================================
@@ -142,7 +143,15 @@ workflow BUILD_REFERENCES {
             !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
             STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
-            ch_starfusion_ref = STARFUSION_BUILD.out.reference
+            if (params.ctatsplicing || params.all) {
+                CTATSPLICING_PREPGENOMELIB(
+                    STARFUSION_BUILD.out.reference
+                )
+                ch_versions = ch_versions.mix(CTATSPLICING_PREPGENOMELIB.out.versions)
+                ch_starfusion_ref = CTATSPLICING_PREPGENOMELIB.out.reference
+            } else {
+                ch_starfusion_ref = STARFUSION_BUILD.out.reference
+            }
     }
     else {
         ch_starfusion_ref = Channel.fromPath(params.starfusion_ref)

From 43b6045db4f743c9b3b0fc001095c4f2f8170d12 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 21 Jan 2025 14:57:07 +0100
Subject: [PATCH 323/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 010d16194..39c7f0ba0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -28,6 +28,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `FUSIONCATCHER_WORKFLOW` [#591](https://github.com/nf-core/rnafusion/pull/591)
 - Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)
 - Add nf-test to local module: `FUSIONINSPECTOR`. [#601](https://github.com/nf-core/rnafusion/pull/601)
+- Added `CTATSPLICING_PREPGENOMELIB` to update the starfusion genome library directory with a cancer splicing index. [#610](https://github.com/nf-core/rnafusion/pull/610)
 
 ### Changed
 

From 472da39e9ad2d5f4b7590012ca332902956d04d2 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 21 Jan 2025 15:09:40 +0100
Subject: [PATCH 324/887] update prepgenomelib resources

---
 conf/modules.config | 5 ++---
 1 file changed, 2 insertions(+), 3 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index c66cafadb..c13cb3d2f 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -374,9 +374,8 @@ process {
     }
 
     withName: 'CTATSPLICING_PREPGENOMELIB' {
-        cpus   = { 24    * task.attempt }
-        memory = { 100.GB * task.attempt }
-        time   = { 2.d  * task.attempt }
+        cpus   = { 1    * task.attempt }
+        memory = { 20.GB * task.attempt }
         publishDir = [
             path: { "${params.genomes_base}/starfusion" },
             mode: params.publish_dir_mode,

From 9fcd72ff7aa4f3d48f6b65e575e5abc039461628 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 Jan 2025 16:14:45 +0100
Subject: [PATCH 325/887] remove md5sums for databases

---
 tests/test_build.nf.test.snap | 3 ---
 1 file changed, 3 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index f2e1bc38f..421d557e7 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -191,13 +191,10 @@
                 "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
                 "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a",
-                "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
-                "hgnc_complete_set.txt:md5,ba66fe5d27ce643b5636a2a85b1b32cb",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
                 "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",

From cf53a214f5ae9791054f169bb0ee3355ad1a7ef4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 Jan 2025 16:50:40 +0100
Subject: [PATCH 326/887] move outdir up

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 1bd38be68..bebb5a8c0 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -15,6 +15,7 @@ params {
     cosmic_username            = null
     cosmic_passwd              = null
     qiagen                     = false
+    outdir                     = null
 
     // MultiQC options
     multiqc_config             = null
@@ -107,7 +108,6 @@ params {
     whitelist                     = null
 
     // Boilerplate options
-    outdir                       = null
     publish_dir_mode             = 'copy'
     email                        = null
     email_on_fail                = null

From 0915989fc0bd19f27f6f9d1a612e1ba5cf33a88b Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 21 Jan 2025 15:55:03 +0000
Subject: [PATCH 327/887] fix stub test snapshot

---
 tests/test_stub.nf.test.snap | 330 ++++++++++++++++++-----------------
 1 file changed, 174 insertions(+), 156 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 3a4257485..cd8cd92cc 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,7 +1,7 @@
 {
     "stub test no fastp trim": {
         "content": [
-            31,
+            32,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -9,6 +9,9 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "CTATSPLICING_PREPGENOMELIB": {
+                    "ctat-splicing": "0.0.2"
+                },
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -224,6 +227,87 @@
                 "references/star/sjdbList.fromGTF.out.tab",
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -266,81 +350,6 @@
                 "star_for_starfusion/test.unmapped_2.fastq.gz",
                 "star_for_starfusion/testXd.out.bam",
                 "starfusion",
-                "starfusion/ctat_genome_lib_build_dir",
-                "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "starfusion/test.starfusion.abridged.coding_effect.tsv",
                 "starfusion/test.starfusion.abridged.tsv",
                 "starfusion/test.starfusion.fusion_predictions.tsv",
@@ -355,13 +364,13 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-07T13:16:02.754052"
+        "timestamp": "2025-01-21T15:53:57.652134536"
     },
     "stub test with fastp trim": {
         "content": [
-            33,
+            34,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -369,6 +378,9 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "CTATSPLICING_PREPGENOMELIB": {
+                    "ctat-splicing": "0.0.2"
+                },
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -599,6 +611,87 @@
                 "references/star/sjdbList.fromGTF.out.tab",
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -641,81 +734,6 @@
                 "star_for_starfusion/test.unmapped_2.fastq.gz",
                 "star_for_starfusion/testXd.out.bam",
                 "starfusion",
-                "starfusion/ctat_genome_lib_build_dir",
-                "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "starfusion/test.starfusion.abridged.coding_effect.tsv",
                 "starfusion/test.starfusion.abridged.tsv",
                 "starfusion/test.starfusion.fusion_predictions.tsv",
@@ -730,8 +748,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-07T13:13:38.690939"
+        "timestamp": "2025-01-21T15:53:10.332117615"
     }
 }
\ No newline at end of file

From 49b2e1d549d22e9aae2a07059f0a11d16a37f0c0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 Jan 2025 17:00:18 +0100
Subject: [PATCH 328/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 010d16194..00339c8ff 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -44,6 +44,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update from ensembl 102 to gencode 46 default references [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Update`FUSIONINSPECTOR` to v2.10.0. [#601](https://github.com/nf-core/rnafusion/pull/601)
 - Remove local module `STARFUSION_DOWNLOAD` [#598](https://github.com/nf-core/rnafusion/pull/598)
+- Fix error message when parameter outdir is missing [#611](https://github.com/nf-core/rnafusion/pull/611)
 
 ### Fixed
 

From 77952f9141d5d9748b279de5726090732ab47f9a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 21 Jan 2025 17:10:11 +0100
Subject: [PATCH 329/887] excude databases subject to quick changes

---
 tests/.nftignore | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/tests/.nftignore b/tests/.nftignore
index 7b5b68596..9d6a156e3 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -3,7 +3,10 @@ references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
 references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
 references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa
 references/hgnc/HGNC-DB-timestamp.txt
+references/hgnc/hgnc_complete_set.txt
 references/fusion_report_db/DB-timestamp.txt
+references/fusion_report_db/fusiongdb2.db
+references/fusion_report_db/mitelman.db
 references/salmon/salmon/ctable.bin
 references/salmon/salmon/pos.bin
 references/salmon/salmon/pre_indexing.log

From 2d5d9bb557a2d3c12e7dbd1536648fdc324b6b06 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 22 Jan 2025 15:11:46 +0000
Subject: [PATCH 330/887] fix: add unstable files to nftignore

---
 tests/.nftignore | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/tests/.nftignore b/tests/.nftignore
index 9d6a156e3..cb651aedf 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -33,3 +33,5 @@ starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
 starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
 references/star/genomeParameters.txt
 starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
+references/fusion_report_db/mitelman.db
+references/fusion_report_db/fusiongdb2.db

From 10d431d184aa768d00799f19eb18362f2386628b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 22 Jan 2025 16:55:30 +0100
Subject: [PATCH 331/887] add param instead of hardcoded url

---
 modules/local/ctatsplicing/prepgenomelib/main.nf | 3 +--
 nextflow.config                                  | 1 +
 nextflow_schema.json                             | 7 +++++++
 subworkflows/local/build_references.nf           | 3 ++-
 4 files changed, 11 insertions(+), 3 deletions(-)

diff --git a/modules/local/ctatsplicing/prepgenomelib/main.nf b/modules/local/ctatsplicing/prepgenomelib/main.nf
index fd7feebc4..0fbf5e9e4 100644
--- a/modules/local/ctatsplicing/prepgenomelib/main.nf
+++ b/modules/local/ctatsplicing/prepgenomelib/main.nf
@@ -8,6 +8,7 @@ process CTATSPLICING_PREPGENOMELIB {
 
     input:
     tuple val(meta), path(genome_lib)
+    path(cancer_intron_tsv)
 
     output:
     tuple val(meta), path(genome_lib, includeInputs:true), emit: reference
@@ -16,8 +17,6 @@ process CTATSPLICING_PREPGENOMELIB {
     script:
     def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz
-
     /usr/local/src/CTAT-SPLICING/prep_genome_lib/ctat-splicing-lib-integration.py \\
         --cancer_introns_tsv cancer_introns.*.tsv.gz \\
         --genome_lib_dir $genome_lib
diff --git a/nextflow.config b/nextflow.config
index d83ceecda..fafd6ac20 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -90,6 +90,7 @@ params {
     starfusion_ref                = "${params.genomes_base}/starfusion/ctat_genome_lib_build_dir"
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
+    ctatsplicing_cancer_introns   = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
 
     // Internal file presence checks
     salmon_index_stub_check       = "${params.genomes_base}/salmon/salmon/complete_ref_lens.bin"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 0f59e2144..aaf8a64ab 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -247,6 +247,13 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to file in starfusion references"
                 },
+                "ctatsplicing_cancer_introns": {
+                    "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "description": "Path to the cancer introns CSV file to create the CTAT-SPLICING reference with",
+                    "default": "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
+                },
                 "starindex": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index c7cb3c31b..6ad2c1906 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -145,7 +145,8 @@ workflow BUILD_REFERENCES {
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (params.ctatsplicing || params.all) {
                 CTATSPLICING_PREPGENOMELIB(
-                    STARFUSION_BUILD.out.reference
+                    STARFUSION_BUILD.out.reference,
+                    params.ctatsplicing_cancer_introns
                 )
                 ch_versions = ch_versions.mix(CTATSPLICING_PREPGENOMELIB.out.versions)
                 ch_starfusion_ref = CTATSPLICING_PREPGENOMELIB.out.reference

From 6d6fbe9aa15412e694a774d5e83788a5c997bbca Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Mon, 27 Jan 2025 14:47:35 +0000
Subject: [PATCH 332/887] Template update for nf-core/tools version 3.2.0

---
 .github/workflows/linting_comment.yml         |  2 +-
 .github/workflows/release-announcements.yml   | 33 -------------------
 .nf-core.yml                                  |  2 +-
 .pre-commit-config.yaml                       |  2 +-
 docs/output.md                                | 11 +++++--
 modules.json                                  |  4 +--
 modules/nf-core/fastqc/main.nf                | 20 +++++------
 modules/nf-core/fastqc/tests/tags.yml         |  2 --
 modules/nf-core/multiqc/environment.yml       |  2 +-
 modules/nf-core/multiqc/main.nf               |  4 +--
 .../nf-core/multiqc/tests/main.nf.test.snap   | 24 +++++++-------
 nextflow.config                               | 16 ++++-----
 ro-crate-metadata.json                        | 28 ++++++++--------
 13 files changed, 60 insertions(+), 90 deletions(-)
 delete mode 100644 modules/nf-core/fastqc/tests/tags.yml

diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 0bed96d36..95b6b6af8 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@80620a5d27ce0ae443b965134db88467fc607b43 # v7
+        uses: dawidd6/action-download-artifact@20319c5641d495c8a52e688b7dc5fada6c3a9fbc # v8
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 450b1d5e4..76a9e67ed 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -27,39 +27,6 @@ jobs:
 
             ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
 
-  send-tweet:
-    runs-on: ubuntu-latest
-
-    steps:
-      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
-        with:
-          python-version: "3.10"
-      - name: Install dependencies
-        run: pip install tweepy==4.14.0
-      - name: Send tweet
-        shell: python
-        run: |
-          import os
-          import tweepy
-
-          client = tweepy.Client(
-              access_token=os.getenv("TWITTER_ACCESS_TOKEN"),
-              access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"),
-              consumer_key=os.getenv("TWITTER_CONSUMER_KEY"),
-              consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"),
-          )
-          tweet = os.getenv("TWEET")
-          client.create_tweet(text=tweet)
-        env:
-          TWEET: |
-            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
-
-            Please see the changelog: ${{ github.event.release.html_url }}
-          TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }}
-          TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }}
-          TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }}
-          TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }}
-
   bsky-post:
     runs-on: ubuntu-latest
     steps:
diff --git a/.nf-core.yml b/.nf-core.yml
index dbf96efef..dae5ba31d 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,7 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
-nf_core_version: 3.1.2
+nf_core_version: 3.2.0
 repository_type: pipeline
 template:
   author: Martin Proks, Annick Renevey
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 9e9f0e1c4..1dec86502 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -7,7 +7,7 @@ repos:
           - prettier@3.2.5
 
   - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
-    rev: "3.0.3"
+    rev: "3.1.2"
     hooks:
       - id: editorconfig-checker
         alias: ec
diff --git a/docs/output.md b/docs/output.md
index f5beb7911..501e19501 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -12,7 +12,8 @@ The directories listed below will be created in the results directory after the
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
-- [FastQC](#fastqc) - Raw read QC- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+- [FastQC](#fastqc) - Raw read QC
+- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
 - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### FastQC
@@ -26,7 +27,9 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 </details>
 
-[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).### MultiQC
+[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
+
+### MultiQC
 
 <details markdown="1">
 <summary>Output files</summary>
@@ -40,7 +43,9 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
 
-Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.### Pipeline information
+Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
+
+### Pipeline information
 
 <details markdown="1">
 <summary>Output files</summary>
diff --git a/modules.json b/modules.json
index d655d7870..296d35429 100644
--- a/modules.json
+++ b/modules.json
@@ -7,12 +7,12 @@
                 "nf-core": {
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "dc94b6ee04a05ddb9f7ae050712ff30a13149164",
+                        "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d",
+                        "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 752c3a10c..033f4154a 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -1,5 +1,5 @@
 process FASTQC {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
@@ -19,30 +19,30 @@ process FASTQC {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def args          = task.ext.args ?: ''
+    def prefix        = task.ext.prefix ?: "${meta.id}"
     // Make list of old name and new name pairs to use for renaming in the bash while loop
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
-    def rename_to = old_new_pairs*.join(' ').join(' ')
+    def rename_to     = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ _old_name, new_name -> new_name }.join(' ')
 
     // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
     // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
     // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
-    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus
+    def memory_in_mb = task.memory ? task.memory.toUnit('MB').toFloat() / task.cpus : null
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
 
     """
-    printf "%s %s\\n" $rename_to | while read old_name new_name; do
+    printf "%s %s\\n" ${rename_to} | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
     done
 
     fastqc \\
-        $args \\
-        --threads $task.cpus \\
-        --memory $fastqc_memory \\
-        $renamed_files
+        ${args} \\
+        --threads ${task.cpus} \\
+        --memory ${fastqc_memory} \\
+        ${renamed_files}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml
deleted file mode 100644
index 7834294ba..000000000
--- a/modules/nf-core/fastqc/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-fastqc:
-  - modules/nf-core/fastqc/**
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index 6f5b867b7..a27122ce1 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -2,4 +2,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.25.1
+  - bioconda::multiqc=1.27
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index cc0643e1d..58d9313c6 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.25.1--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.25.1--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.27--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.27--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index 2fcbb5ff7..7b7c13220 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
+                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-02T17:51:46.317523"
+        "timestamp": "2025-01-27T09:29:57.631982377"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
+                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-02T17:52:20.680978"
+        "timestamp": "2025-01-27T09:30:34.743726958"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
+                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-02T17:52:09.185842"
+        "timestamp": "2025-01-27T09:30:21.44383553"
     }
 }
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 1057767d5..a309f87e6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -195,14 +195,14 @@ env {
 }
 
 // Set bash options
-process.shell = """\
-bash
-
-set -e # Exit if a tool returns a non-zero status/exit code
-set -u # Treat unset variables and parameters as an error
-set -o pipefail # Returns the status of the last command to exit with a non-zero status or zero if all successfully execute
-set -C # No clobber - prevent output redirection from overwriting files.
-"""
+process.shell = [
+    "bash",
+    "-C",         // No clobber - prevent output redirection from overwriting files.
+    "-e",         // Exit if a tool returns a non-zero status/exit code
+    "-u",         // Treat unset variables and parameters as an error
+    "-o",         // Returns the status of the last command to exit..
+    "pipefail"    //   ..with a non-zero status or zero if all successfully execute
+]
 
 // Disable process selector warnings by default. Use debug profile to enable warnings.
 nextflow.enable.configProcessNamesValidation = false
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 1314af32a..972b1a807 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,7 +22,7 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
-            "datePublished": "2025-01-20T14:36:55+00:00",
+            "datePublished": "2025-01-27T14:47:28+00:00",
             "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#f6260e6b-9f65-45aa-9ada-0e886fa2fb70"
+                    "@id": "#472b4f7d-c1ad-4a49-9ebc-abac03e60cd0"
                 }
             ],
             "name": "nf-core/rnafusion"
@@ -127,17 +127,17 @@
                     "@id": "#phil.ewels@scilifelab.se"
                 },
                 {
-                    "@id": "https://orcid.org/0000-0001-9017-591X"
+                    "@id": "#max.u.garcia@gmail.com"
                 },
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
                 },
                 {
-                    "@id": "#max.u.garcia@gmail.com"
+                    "@id": "https://orcid.org/0000-0001-9017-591X"
                 }
             ],
             "dateCreated": "",
-            "dateModified": "2025-01-20T14:36:55Z",
+            "dateModified": "2025-01-27T14:47:28Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
             "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
             "license": ["MIT"],
@@ -169,11 +169,11 @@
             "version": "!>=24.04.2"
         },
         {
-            "@id": "#f6260e6b-9f65-45aa-9ada-0e886fa2fb70",
+            "@id": "#472b4f7d-c1ad-4a49-9ebc-abac03e60cd0",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#c5ee26a1-ec22-464a-b17b-3a0c5dcf6e02"
+                    "@id": "#a52cf27d-3699-475d-b4c8-2eb08a2f0245"
                 }
             ],
             "mainEntity": {
@@ -182,7 +182,7 @@
             "name": "Test suite for nf-core/rnafusion"
         },
         {
-            "@id": "#c5ee26a1-ec22-464a-b17b-3a0c5dcf6e02",
+            "@id": "#a52cf27d-3699-475d-b4c8-2eb08a2f0245",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/rnafusion",
             "resource": "repos/nf-core/rnafusion/actions/workflows/ci.yml",
@@ -317,10 +317,10 @@
             "name": "Phil Ewels"
         },
         {
-            "@id": "https://orcid.org/0000-0001-9017-591X",
+            "@id": "#max.u.garcia@gmail.com",
             "@type": "Person",
-            "email": "rickard.hammaren@scilifelab.se",
-            "name": "Rickard Hammar\u00e9n"
+            "email": "max.u.garcia@gmail.com",
+            "name": "Maxime Garcia"
         },
         {
             "@id": "https://orcid.org/0000-0002-8178-3128",
@@ -329,10 +329,10 @@
             "name": "Martin Proks"
         },
         {
-            "@id": "#max.u.garcia@gmail.com",
+            "@id": "https://orcid.org/0000-0001-9017-591X",
             "@type": "Person",
-            "email": "max.u.garcia@gmail.com",
-            "name": "Maxime Garcia"
+            "email": "rickard.hammaren@scilifelab.se",
+            "name": "Rickard Hammar\u00e9n"
         }
     ]
 }

From 659fa0bc733b298724712bba50575ea97d56e6d6 Mon Sep 17 00:00:00 2001
From: MacBook Air <macbookair@MacBooks-Air.attlocal.net>
Date: Thu, 20 Feb 2025 17:04:09 -0800
Subject: [PATCH 333/887] docs: update fusionreport score calculation
 documentation

- Add detailed explanation of new scoring system
- Document 80/20 weight distribution between tool detection and database hits
- Add formula for score calculation
---
 docs/output.md | 14 +++++++++-----
 1 file changed, 9 insertions(+), 5 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index ad3c78e36..1664babfb 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -264,13 +264,17 @@ The weights for databases are as follows:
 - MITELMAN (50)
 - FusionGDB2 (0)
 
-The final formula for calculating score is:
+The score is calculated using two components:
 
-$$
-score = 0.5 * \sum_{tool}^{tools} f(fusion, tool)*w(tool) + 0.5 * \sum_{db}^{dbs} g(fusion, db)*w(db)
-$$
+1. Tool Detection (80% of total score)
+   - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
+   - This reflects how many of the active tools found the fusion
 
-All tools have the same weight.
+2. Database Hits (20% of total score)
+   - Based on database matches using weights above
+   - Calculated as: (number of database hits) / (total possible database hits)
+
+Final score = (0.8 × Tool Detection Score) + (0.2 × Database Hits Score)
 
 ### Salmon
 

From 214b70b52ee1c9e538806ec8a831513ad9b26e9f Mon Sep 17 00:00:00 2001
From: MacBook Air <macbookair@MacBooks-Air.attlocal.net>
Date: Tue, 25 Feb 2025 11:43:33 -0800
Subject: [PATCH 334/887] Apply pre-commit fixes and update changelog

---
 CHANGELOG.md   | 1 +
 docs/output.md | 1 +
 2 files changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index cdc774e1d..c3220386a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -29,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)
 - Add nf-test to local module: `FUSIONINSPECTOR`. [#601](https://github.com/nf-core/rnafusion/pull/601)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
+- Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
 
 ### Changed
 
diff --git a/docs/output.md b/docs/output.md
index 1664babfb..3698f7a69 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -267,6 +267,7 @@ The weights for databases are as follows:
 The score is calculated using two components:
 
 1. Tool Detection (80% of total score)
+
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 

From e84787b83667784cdaf58e3b842b5fb625b45cf0 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 11 Mar 2025 19:06:39 +0000
Subject: [PATCH 335/887] feat: add nf-test to `arriba_visualisation`

---
 modules/local/arriba/visualisation/main.nf    |  3 +-
 .../arriba/visualisation/tests/main.nf.test   | 83 +++++++++++++++++++
 .../visualisation/tests/main.nf.test.snap     | 49 +++++++++++
 3 files changed, 134 insertions(+), 1 deletion(-)
 create mode 100644 modules/local/arriba/visualisation/tests/main.nf.test
 create mode 100644 modules/local/arriba/visualisation/tests/main.nf.test.snap

diff --git a/modules/local/arriba/visualisation/main.nf b/modules/local/arriba/visualisation/main.nf
index f1aa097b6..293247328 100644
--- a/modules/local/arriba/visualisation/main.nf
+++ b/modules/local/arriba/visualisation/main.nf
@@ -23,14 +23,15 @@ process ARRIBA_VISUALISATION {
     script:
     def args = task.ext.args ?: ''
     def arg_cytobands = cytobands ? " --cytobands=$cytobands" : ""
+    def arg_alignment = bam ? " --alignments=$bam" : ""
     def arg_protein_domains = protein_domains ? "--proteinDomains=$protein_domains" : ""
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     draw_fusions.R \\
         --fusions=$fusions \\
-        --alignments=$bam \\
         --output=${prefix}.pdf \\
         --annotation=${gtf} \\
+        $arg_alignment \\
         $arg_cytobands \\
         $arg_protein_domains \\
         $args
diff --git a/modules/local/arriba/visualisation/tests/main.nf.test b/modules/local/arriba/visualisation/tests/main.nf.test
new file mode 100644
index 000000000..1a18b0db3
--- /dev/null
+++ b/modules/local/arriba/visualisation/tests/main.nf.test
@@ -0,0 +1,83 @@
+nextflow_process {
+
+    name "Test Process ARRIBA_VISUALISATION"
+    script "../main.nf"
+    process "ARRIBA_VISUALISATION"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "arriba"
+    tag "arriba/visualisation"
+
+    test("ARRIBA_VISUALISATION") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [],
+                    [],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv", checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_gtf' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'test_protein_domains' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true)
+                ]
+                input[3] = [
+                    [ id:'test_cytobands' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert file(process.out.pdf[0][1]).name },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("ARRIBA_VISUALISATION stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam", checkIfExists: true),
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai", checkIfExists: true),
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv", checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_gtf' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'test_protein_domains' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true)
+                ]
+                input[3] = [
+                    [ id:'test_cytobands' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/local/arriba/visualisation/tests/main.nf.test.snap b/modules/local/arriba/visualisation/tests/main.nf.test.snap
new file mode 100644
index 000000000..7ed24df79
--- /dev/null
+++ b/modules/local/arriba/visualisation/tests/main.nf.test.snap
@@ -0,0 +1,49 @@
+{
+    "versions": {
+        "content": [
+            [
+                "versions.yml:md5,77b78715defd458bd9e742e58e39cd42"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-11T19:05:38.906558079"
+    },
+    "ARRIBA_VISUALISATION stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,77b78715defd458bd9e742e58e39cd42"
+                ],
+                "pdf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,77b78715defd458bd9e742e58e39cd42"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-11T19:05:48.424051167"
+    }
+}
\ No newline at end of file

From d1cc0da9f07d55ca67090c1038eeb87ec2876c73 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 11 Mar 2025 19:11:08 +0000
Subject: [PATCH 336/887] docs: update changelog

---
 CHANGELOG.md | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c3220386a..fff397252 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -29,7 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)
 - Add nf-test to local module: `FUSIONINSPECTOR`. [#601](https://github.com/nf-core/rnafusion/pull/601)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
-- Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
+- Add nf-test to local module: `ARRIBA_VISUALISATION`. [#625](https://github.com/nf-core/rnafusion/pull/625)
 
 ### Changed
 
@@ -47,6 +47,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update`FUSIONINSPECTOR` to v2.10.0. [#601](https://github.com/nf-core/rnafusion/pull/601)
 - Remove local module `STARFUSION_DOWNLOAD` [#598](https://github.com/nf-core/rnafusion/pull/598)
 - Fix error message when parameter outdir is missing [#611](https://github.com/nf-core/rnafusion/pull/611)
+- Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
 
 ### Fixed
 

From 6aae0c693b37bc38b913b53f4889ebdca6619bcb Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 17 Mar 2025 15:17:08 +0100
Subject: [PATCH 337/887] update star module

---
 modules.json                                  |  2 +-
 modules/nf-core/star/align/environment.yml    |  6 +-
 modules/nf-core/star/align/main.nf            | 13 +--
 .../star/align/tests/main.nf.test.snap        | 90 +++++++++----------
 4 files changed, 57 insertions(+), 54 deletions(-)

diff --git a/modules.json b/modules.json
index 01e049b64..b4ca9cafe 100644
--- a/modules.json
+++ b/modules.json
@@ -118,7 +118,7 @@
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "a5ad53288c79fa52c5ae708c317e09ec2dd149ab",
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
                         "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
diff --git a/modules/nf-core/star/align/environment.yml b/modules/nf-core/star/align/environment.yml
index 7c57530a3..91e37ae12 100644
--- a/modules/nf-core/star/align/environment.yml
+++ b/modules/nf-core/star/align/environment.yml
@@ -1,9 +1,11 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 
 dependencies:
-  - bioconda::htslib=1.20
-  - bioconda::samtools=1.20
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
   - bioconda::star=2.7.11b
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index 417071ba9..22a52184d 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -4,8 +4,8 @@ process STAR_ALIGN {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b4/b425bc2a95806d878993f9a66dae3ae80ac2dafff4c208c5ae01b7a90a32fa91/data' :
-        'community.wave.seqera.io/library/star_samtools_htslib_gawk:10c6e8c834460019' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/26/268b4c9c6cbf8fa6606c9b7fd4fafce18bf2c931d1a809a0ce51b105ec06c89d/data' :
+        'community.wave.seqera.io/library/htslib_samtools_star_gawk:ae438e9a604351a4' }"
 
     input:
     tuple val(meta), path(reads, stageAs: "input*/*")
@@ -41,12 +41,13 @@ process STAR_ALIGN {
     script:
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
-    def reads1 = [], reads2 = []
+    def reads1 = []
+    def reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
-    def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""
-    def seq_center      = seq_center ? "'CN:$seq_center'" : ""
-    attrRG          = args.contains("--outSAMattrRGline") ? "" : "--outSAMattrRGline 'ID:$prefix' $seq_center 'SM:$prefix' $seq_platform"
+    def seq_platform_arg  = seq_platform ? "'PL:$seq_platform'" : ""
+    def seq_center_arg    = seq_center ? "'CN:$seq_center'" : ""
+    attrRG          = args.contains("--outSAMattrRGline") ? "" : "--outSAMattrRGline 'ID:$prefix' $seq_center_arg 'SM:$prefix' $seq_platform_arg"
     def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
     mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
     """
diff --git a/modules/nf-core/star/align/tests/main.nf.test.snap b/modules/nf-core/star/align/tests/main.nf.test.snap
index b533fb8b4..a1ec3a3d0 100644
--- a/modules/nf-core/star/align/tests/main.nf.test.snap
+++ b/modules/nf-core/star/align/tests/main.nf.test.snap
@@ -97,7 +97,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -307,7 +307,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -321,10 +321,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:08.738074176"
+        "timestamp": "2024-12-11T16:28:39.242074494"
     },
     "homo_sapiens - paired_end - arriba - stub": {
         "content": [
@@ -424,7 +424,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -634,7 +634,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -648,10 +648,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:36.122131869"
+        "timestamp": "2024-12-11T16:29:08.392620556"
     },
     "homo_sapiens - single_end": {
         "content": [
@@ -703,14 +703,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:01:22.197991909"
+        "timestamp": "2024-12-11T16:21:34.549483887"
     },
     "homo_sapiens - paired_end": {
         "content": [
@@ -762,14 +762,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:02:06.988663857"
+        "timestamp": "2024-12-11T16:22:17.011253146"
     },
     "homo_sapiens - paired_end - multiple - stub": {
         "content": [
@@ -869,7 +869,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -1079,7 +1079,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -1093,10 +1093,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:10:12.005468781"
+        "timestamp": "2024-12-11T16:29:31.072104862"
     },
     "homo_sapiens - paired_end - multiple": {
         "content": [
@@ -1148,14 +1148,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:08:54.877286681"
+        "timestamp": "2024-12-11T16:28:26.645008336"
     },
     "homo_sapiens - paired_end - stub": {
         "content": [
@@ -1255,7 +1255,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -1465,7 +1465,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -1479,10 +1479,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:20.911466345"
+        "timestamp": "2024-12-11T16:28:53.816280805"
     },
     "homo_sapiens - paired_end - starfusion": {
         "content": [
@@ -1525,14 +1525,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:07:25.0639914"
+        "timestamp": "2024-12-11T16:27:14.136111964"
     },
     "homo_sapiens - paired_end - arriba": {
         "content": [
@@ -1574,14 +1574,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:04:00.685784211"
+        "timestamp": "2024-12-11T16:24:00.829462526"
     },
     "homo_sapiens - paired_end - starfusion - stub": {
         "content": [
@@ -1681,7 +1681,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -1891,7 +1891,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -1905,9 +1905,9 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:53.173671551"
+        "timestamp": "2024-12-11T16:29:20.015372487"
     }
 }
\ No newline at end of file

From a3bb3d98ec82e7c5075ef17524917eabdb0f7212 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 17 Mar 2025 16:50:21 +0100
Subject: [PATCH 338/887] first implementation of the star consolidation

---
 conf/modules.config                           | 136 ++++--------------
 modules.json                                  |   5 +
 .../nf-core/samtools/convert/environment.yml  |   8 ++
 modules/nf-core/samtools/convert/main.nf      |  60 ++++++++
 modules/nf-core/samtools/convert/meta.yml     | 103 +++++++++++++
 .../samtools/convert/tests/main.nf.test       | 107 ++++++++++++++
 .../samtools/convert/tests/main.nf.test.snap  | 131 +++++++++++++++++
 .../nf-core/samtools/convert/tests/tags.yml   |   2 +
 nextflow.config                               |   6 +-
 nextflow_schema.json                          |   6 +-
 subworkflows/local/arriba_workflow/main.nf    |  53 +------
 subworkflows/local/fastq_align_star/main.nf   |  58 ++++++++
 .../local/starfusion_workflow/main.nf         |  41 +-----
 workflows/rnafusion.nf                        |  81 +++++++----
 14 files changed, 569 insertions(+), 228 deletions(-)
 create mode 100644 modules/nf-core/samtools/convert/environment.yml
 create mode 100644 modules/nf-core/samtools/convert/main.nf
 create mode 100644 modules/nf-core/samtools/convert/meta.yml
 create mode 100644 modules/nf-core/samtools/convert/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/convert/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/convert/tests/tags.yml
 create mode 100644 subworkflows/local/fastq_align_star/main.nf

diff --git a/conf/modules.config b/conf/modules.config
index 967e820a7..68e2e3478 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -45,25 +45,13 @@ process {
         ]
     }
 
-    withName: '.*ARRIBA_WORKFLOW:.*:CTATSPLICING_STARTOCANCERINTRONS' {
+    withName: 'CTATSPLICING_STARTOCANCERINTRONS' {
         ext.args = {[
             bam ? "--vis" : "",
             "--sample_name ${meta.id}",
         ].join(" ")}
         publishDir = [
-            path: { "${params.outdir}/ctatsplicing/arriba" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
-    withName: '.*STARFUSION_WORKFLOW:.*:CTATSPLICING_STARTOCANCERINTRONS' {
-        ext.args = {[
-            bam ? "--vis" : "",
-            "--sample_name ${meta.id}",
-        ].join(" ")}
-        publishDir = [
-            path: { "${params.outdir}/ctatsplicing/starfusion" },
+            path: { "${params.outdir}/ctatsplicing" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -237,115 +225,49 @@ process {
         ]
     }
 
-    withName: 'SAMTOOLS_INDEX_FOR_ARRIBA' {
-        ext.prefix = { "${meta.id}_star_for_arriba_sorted" }
-        publishDir = [
-            path: { "${params.outdir}/cram_arriba" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
-    withName: 'SAMTOOLS_SORT_FOR_ARRIBA' {
-        ext.prefix = { "${meta.id}_star_for_arriba_sorted" }
-        publishDir = [
-            path: { "${params.outdir}/cram_arriba" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
-    withName: 'SAMTOOLS_VIEW_FOR_ARRIBA' {
-        ext.args = { "--output-fmt cram" }
-        ext.prefix = { "${meta.id}_star_for_arriba_sorted" }
-        publishDir = [
-            path: { "${params.outdir}/cram_arriba" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
-    withName: 'SAMTOOLS_INDEX_FOR_STARFUSION' {
-        publishDir = [
-            path: { "${params.outdir}/star_for_starfusion" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
-    withName: 'SAMTOOLS_INDEX_FOR_STARFUSION_CRAM' {
-        ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
+    withName: 'SAMTOOLS_CONVERT' {
+        ext.prefix = { "${meta.id}.Aligned.sortedByCoord.out" }
         publishDir = [
-            path: { "${params.outdir}/cram_starfusion" },
+            path: { "${params.outdir}/cram" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
 
-    withName: 'SAMTOOLS_VIEW_FOR_STARFUSION' {
-        ext.args = { "--output-fmt cram" }
-        ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
+    withName: 'SAMTOOLS_INDEX' {
         publishDir = [
-            path: { "${params.outdir}/cram_starfusion" },
+            path: { "${params.outdir}/star" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
 
-    withName: 'STAR_FOR_ARRIBA' {
-        publishDir = [
-            path: { "${params.outdir}/star_for_arriba" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
-        ]
-        ext.args = '--readFilesCommand zcat \
-        --outSAMtype BAM SortedByCoordinate \
-        --outSAMunmapped Within \
-        --outBAMcompression 0 \
-        --outFilterMultimapNmax 50 \
-        --peOverlapNbasesMin 10 \
-        --alignSplicedMateMapLminOverLmate 0.5 \
-        --alignSJstitchMismatchNmax 5 -1 5 5 \
-        --chimSegmentMin 10 \
-        --chimOutType WithinBAM HardClip Junctions \
-        --chimJunctionOverhangMin 10 \
-        --chimScoreDropMax 30 \
-        --chimScoreJunctionNonGTAG 0 \
-        --chimScoreSeparation 1 \
-        --chimSegmentReadGapMax 3 \
-        --chimMultimapNmax 50'
-    }
-
-    withName: 'STAR_FOR_STARFUSION' {
+    withName: 'STAR_ALIGN' {
         publishDir = [
-            path: { "${params.outdir}/star_for_starfusion" },
+            path: { "${params.outdir}/star" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
-        ext.args = '--twopassMode Basic \
-        --outReadsUnmapped None \
-        --readFilesCommand zcat \
-        --outSAMtype BAM SortedByCoordinate \
-        --outSAMstrandField intronMotif \
-        --outSAMunmapped Within \
-        --chimSegmentMin 12 \
-        --chimJunctionOverhangMin 8 \
-        --chimOutJunctionFormat 1 \
-        --alignSJDBoverhangMin 10 \
-        --alignMatesGapMax 100000 \
-        --alignIntronMax 100000 \
-        --alignSJstitchMismatchNmax 5 -1 5 5 \
-        --chimMultimapScoreRange 3 \
-        --chimScoreJunctionNonGTAG -4 \
-        --chimMultimapNmax 20 \
-        --chimNonchimScoreDropMin 10 \
-        --peOverlapNbasesMin 12 \
-        --peOverlapMMp 0.1 \
-        --alignInsertionFlush Right \
-        --alignSplicedMateMapLminOverLmate 0 \
-        --alignSplicedMateMapLmin 30 \
-        --chimOutType Junctions \
-        --quantMode GeneCounts'
+        ext.args = '--outReadsUnmapped None  \
+            --outSAMstrandField intronMotif \
+            --chimOutJunctionFormat 1 \
+            --twopassMode None \
+            --outFilterMultimapNmax 50 \
+            --chimMultimapNmax 50 \
+            --quantMode GeneCounts \
+            --outSAMunmapped Within \
+            --readFilesCommand zcat  \
+            --alignSJstitchMismatchNmax 5 -1 5 5 \
+            --outSAMtype BAM SortedByCoordinate \
+            --chimSegmentMin 10 \
+            --peOverlapNbasesMin 10 \
+            --alignSplicedMateMapLminOverLmate 0.5 \
+            --chimJunctionOverhangMin 10 \
+            --chimScoreJunctionNonGTAG 0 \
+            --chimScoreDropMax 30 \
+            --chimScoreSeparation 1  \
+            --chimSegmentReadGapMax 3 \
+            --chimOutType Junctions WithinBAM'
     }
 
     withName: 'STAR_GENOMEGENERATE' {
diff --git a/modules.json b/modules.json
index b4ca9cafe..79e06b4e9 100644
--- a/modules.json
+++ b/modules.json
@@ -96,6 +96,11 @@
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
                         "installed_by": ["modules"]
                     },
+                    "samtools/convert": {
+                        "branch": "master",
+                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
+                        "installed_by": ["modules"]
+                    },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
diff --git a/modules/nf-core/samtools/convert/environment.yml b/modules/nf-core/samtools/convert/environment.yml
new file mode 100644
index 000000000..62054fc97
--- /dev/null
+++ b/modules/nf-core/samtools/convert/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/convert/main.nf b/modules/nf-core/samtools/convert/main.nf
new file mode 100644
index 000000000..cf9253d10
--- /dev/null
+++ b/modules/nf-core/samtools/convert/main.nf
@@ -0,0 +1,60 @@
+process SAMTOOLS_CONVERT {
+    tag "$meta.id"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+
+    input:
+    tuple val(meta), path(input), path(index)
+    tuple val(meta2), path(fasta)
+    tuple val(meta3), path(fai)
+
+    output:
+    tuple val(meta), path("*.bam")  , emit: bam ,   optional: true
+    tuple val(meta), path("*.cram") , emit: cram,   optional: true
+    tuple val(meta), path("*.bai")  , emit: bai ,   optional: true
+    tuple val(meta), path("*.crai") , emit: crai,   optional: true
+    path  "versions.yml"            , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args  ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def output_extension = input.getExtension() == "bam" ? "cram" : "bam"
+
+    """
+    samtools view \\
+        --threads ${task.cpus} \\
+        --reference ${fasta} \\
+        $args \\
+        $input \\
+        -o ${prefix}.${output_extension}
+
+    samtools index -@${task.cpus} ${prefix}.${output_extension}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def output_extension = input.getExtension() == "bam" ? "cram" : "bam"
+    def index_extension = output_extension == "bam" ? "bai" : "crai"
+
+    """
+    touch ${prefix}.${output_extension}
+    touch ${prefix}.${index_extension}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/samtools/convert/meta.yml b/modules/nf-core/samtools/convert/meta.yml
new file mode 100644
index 000000000..d5bfa161b
--- /dev/null
+++ b/modules/nf-core/samtools/convert/meta.yml
@@ -0,0 +1,103 @@
+name: samtools_convert
+description: convert and then index CRAM -> BAM or BAM -> CRAM file
+keywords:
+  - view
+  - index
+  - bam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+      identifier: biotools:samtools
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM file
+        pattern: "*.{bam,cram}"
+    - index:
+        type: file
+        description: BAM/CRAM index file
+        pattern: "*.{bai,crai}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - fasta:
+        type: file
+        description: Reference file to create the CRAM file
+        pattern: "*.{fasta,fa}"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - fai:
+        type: file
+        description: Reference index file to create the CRAM file
+        pattern: "*.{fai}"
+output:
+  - bam:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bam":
+          type: file
+          description: filtered/converted BAM file
+          pattern: "*{.bam}"
+  - cram:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.cram":
+          type: file
+          description: filtered/converted CRAM file
+          pattern: "*{cram}"
+  - bai:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bai":
+          type: file
+          description: filtered/converted BAM index
+          pattern: "*{.bai}"
+  - crai:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.crai":
+          type: file
+          description: filtered/converted CRAM index
+          pattern: "*{.crai}"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+authors:
+  - "@FriederikeHanssen"
+  - "@maxulysse"
+maintainers:
+  - "@FriederikeHanssen"
+  - "@maxulysse"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test b/modules/nf-core/samtools/convert/tests/main.nf.test
new file mode 100644
index 000000000..91a0c69ea
--- /dev/null
+++ b/modules/nf-core/samtools/convert/tests/main.nf.test
@@ -0,0 +1,107 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_CONVERT"
+    script "../main.nf"
+    process "SAMTOOLS_CONVERT"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/convert"
+
+    test("sarscov2 - [bam, bai], fasta, fai") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = Channel.of([
+                    [ id:'fai' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.cram[0][1]).name).match("bam_to_cram_alignment") },
+                { assert snapshot(file(process.out.crai[0][1]).name).match("bam_to_cram_index") },
+                { assert snapshot(process.out.versions).match("bam_to_cram_versions") }
+            )
+        }
+    }
+
+    test("homo_sapiens - [cram, crai], fasta, fai") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = Channel.of([
+                    [ id:'fai' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_alignment") },
+                { assert snapshot(file(process.out.bai[0][1]).name).match("cram_to_bam_alignment_index") },
+                { assert snapshot(process.out.versions).match("cram_to_bam_versions") }
+            )
+        }
+    }
+
+    test("sarscov2 - [bam, bai], fasta, fai - stub") {
+        
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [id: 'test', single_end: false], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = Channel.of([
+                    [ id:'fai' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match("stub") }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test.snap b/modules/nf-core/samtools/convert/tests/main.nf.test.snap
new file mode 100644
index 000000000..a021254e8
--- /dev/null
+++ b/modules/nf-core/samtools/convert/tests/main.nf.test.snap
@@ -0,0 +1,131 @@
+{
+    "cram_to_bam_alignment": {
+        "content": [
+            "test.bam"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-06T11:14:51.300147176"
+    },
+    "bam_to_cram_alignment": {
+        "content": [
+            "test.cram"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-06T11:14:36.625470184"
+    },
+    "cram_to_bam_versions": {
+        "content": [
+            [
+                "versions.yml:md5,5bc6eb42ab2a1ea6661f8ee998467ad6"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T07:52:35.516411351"
+    },
+    "bam_to_cram_versions": {
+        "content": [
+            [
+                "versions.yml:md5,5bc6eb42ab2a1ea6661f8ee998467ad6"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T07:52:24.694454205"
+    },
+    "stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,5bc6eb42ab2a1ea6661f8ee998467ad6"
+                ],
+                "bai": [
+                    
+                ],
+                "bam": [
+                    
+                ],
+                "crai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "cram": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5bc6eb42ab2a1ea6661f8ee998467ad6"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T07:52:45.799885099"
+    },
+    "bam_to_cram_index": {
+        "content": [
+            "test.cram.crai"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-06T11:14:36.640009334"
+    },
+    "cram_to_bam_alignment_index": {
+        "content": [
+            "test.bam.bai"
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-03-06T11:14:51.304477426"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/convert/tests/tags.yml b/modules/nf-core/samtools/convert/tests/tags.yml
new file mode 100644
index 000000000..030d5eb5e
--- /dev/null
+++ b/modules/nf-core/samtools/convert/tests/tags.yml
@@ -0,0 +1,2 @@
+samtools/convert:
+  - "modules/nf-core/samtools/convert/**"
diff --git a/nextflow.config b/nextflow.config
index f1e66fa42..9fbc0dbb6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -43,12 +43,12 @@ params {
     adapter_fasta              = []
 
     // Compression
-    cram                       = []
+    cram                       = false
 
     // Alignment options
     star_ignore_sjdbgtf                  = false
-    seq_center                           = null
-    seq_platform                         = null
+    seq_center                           = ""
+    seq_platform                         = ""
     fusioncatcher_limitSjdbInsertNsj     = 2000000
     fusioninspector_limitSjdbInsertNsj   = 1000000
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 0f59e2144..cfe294653 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -309,8 +309,8 @@
             "description": "Option to compress BAM files to CRAM.",
             "properties": {
                 "cram": {
-                    "type": "string",
-                    "description": "List of tools for which to compress BAM file to CRAM,default: [], options: arriba, starfusion. Leave no space between options",
+                    "type": "boolean",
+                    "description": "Output CRAM files instead of BAM files.",
                     "fa_icon": "fas fa-cut"
                 }
             }
@@ -534,14 +534,12 @@
                 "seq_center": {
                     "type": "string",
                     "description": "Sequencing center",
-                    "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This will reported in the BAM header as CN"
                 },
                 "seq_platform": {
                     "type": "string",
                     "description": "Sequencing platform",
-                    "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This will reported in the BAM header as PL."
                 },
diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 84ba3604f..a9a0d9cc9 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -1,59 +1,26 @@
 include { ARRIBA_ARRIBA                               } from '../../../modules/nf-core/arriba/arriba/main'
-include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_ARRIBA } from '../../../modules/nf-core/samtools/index/main'
-include { SAMTOOLS_SORT as SAMTOOLS_SORT_FOR_ARRIBA   } from '../../../modules/nf-core/samtools/sort/main'
-include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_ARRIBA   } from '../../../modules/nf-core/samtools/view/main'
-include { STAR_ALIGN as STAR_FOR_ARRIBA               } from '../../../modules/nf-core/star/align/main'
-
-include { CTATSPLICING_WORKFLOW         }   from '../ctatsplicing_workflow'
 
 workflow ARRIBA_WORKFLOW {
     take:
-        reads                           // channel [ meta, [ fastqs ]         ]
+        reads                           // channel [ meta, [ bam ]            ]
         ch_gtf                          // channel [ meta, path_gtf           ]
         ch_fasta                        // channel [ meta, path_fasta         ]
-        ch_starindex_ref                // channel [ meta, path_index         ]
         ch_arriba_ref_blacklist         // channel [ meta, path_blacklist     ]
         ch_arriba_ref_cytobands         // channel [ meta, path_cytobands     ]
         ch_arriba_ref_known_fusions     // channel [ meta, path_known_fusions ]
         ch_arriba_ref_protein_domains   // channel [ meta, path_proteins      ]
-        ch_starfusion_ref               // channel [ meta, path_starfusion_ref ]
         arriba                          // boolean
         all                             // boolean
         fusioninspector_only            // boolean
-        star_ignore_sjdbgtf             // boolean
-        ctatsplicing                    // boolean
-        seq_center                      // string
         arriba_fusions                  // path
-        cram                            // array
 
     main:
 
         def ch_versions   = Channel.empty()
-        def ch_cram_index = Channel.empty()
         def ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         if (( arriba || all ) && !fusioninspector_only) {
 
-            STAR_FOR_ARRIBA(
-                reads,
-                ch_starindex_ref,
-                ch_gtf,
-                star_ignore_sjdbgtf,
-                '',
-                seq_center
-            )
-            ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
-
-            if ( ctatsplicing || all ) {
-                CTATSPLICING_WORKFLOW(
-                    STAR_FOR_ARRIBA.out.spl_junc_tab,
-                    STAR_FOR_ARRIBA.out.junction,
-                    STAR_FOR_ARRIBA.out.bam,
-                    ch_starfusion_ref
-                )
-                ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
-            }
-
             if ( arriba_fusions ) {
 
                 ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
@@ -63,7 +30,7 @@ workflow ARRIBA_WORKFLOW {
             } else {
 
                 ARRIBA_ARRIBA (
-                    STAR_FOR_ARRIBA.out.bam,
+                    reads,
                     ch_fasta,
                     ch_gtf,
                     ch_arriba_ref_blacklist,
@@ -78,21 +45,6 @@ workflow ARRIBA_WORKFLOW {
                 ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ it -> return it[1] }
             }
 
-            if ( cram.contains('arriba') ) {
-
-                SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam, ch_fasta)
-                ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions )
-
-                SAMTOOLS_VIEW_FOR_ARRIBA(SAMTOOLS_SORT_FOR_ARRIBA.out.bam.map { meta, bam -> [ meta, bam, [] ] }, ch_fasta, [])
-                ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_ARRIBA.out.versions )
-
-                SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_VIEW_FOR_ARRIBA.out.cram)
-                ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions )
-
-                // Join cram and index files
-                ch_cram_index = SAMTOOLS_VIEW_FOR_ARRIBA.out.cram.join(SAMTOOLS_INDEX_FOR_ARRIBA.out.crai)
-            }
-
         } else {
 
             ch_arriba_fusions = reads
@@ -105,7 +57,6 @@ workflow ARRIBA_WORKFLOW {
     emit:
         fusions      = ch_arriba_fusions        // channel [ meta, path_fusions   ]
         fusions_fail = ch_arriba_fusion_fail    // channel [ path, fusions_failed ]
-        cram_index   = ch_cram_index            // channel [ meta, cram, crai     ]
         versions     = ch_versions              // channel [ versions             ]
     }
 
diff --git a/subworkflows/local/fastq_align_star/main.nf b/subworkflows/local/fastq_align_star/main.nf
new file mode 100644
index 000000000..2477b31bd
--- /dev/null
+++ b/subworkflows/local/fastq_align_star/main.nf
@@ -0,0 +1,58 @@
+include { SAMTOOLS_CONVERT  } from '../../../modules/nf-core/samtools/convert/main'
+include { STAR_ALIGN        } from '../../../modules/nf-core/star/align/main'
+include { SAMTOOLS_INDEX    } from '../../../modules/nf-core/samtools/index/main.nf'
+
+workflow FASTQ_ALIGN_STAR {
+    take:
+    reads           // channel: [ meta, [ fastq1, fastq2 ]]
+    index           // channel: [ meta, index ]
+    gtf             // channel: [ meta, gtf ]
+    fasta           // channel: [ meta, fasta ]
+    fai             // channel: [ meta, fai ]
+    ignore_sjdbgtf  // boolean
+    seq_platform    // string
+    seq_center      // string
+    cram            // boolean: Create CRAM files
+
+    main:
+    def ch_versions = Channel.empty()
+    STAR_ALIGN(
+        reads,
+        index,
+        gtf,
+        ignore_sjdbgtf,
+        seq_platform,
+        seq_center
+    )
+    ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first())
+
+    SAMTOOLS_INDEX(
+        STAR_ALIGN.out.bam_sorted
+    )
+    ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first())
+
+    ch_bam_bai = STAR_ALIGN.out.bam_sorted
+        .join(SAMTOOLS_INDEX.out.bai, failOnMismatch:true, failOnDuplicate:true)
+
+    def ch_cram_crai = Channel.empty()
+    if(cram) {
+        SAMTOOLS_CONVERT(
+            STAR_ALIGN.out.bam_sorted.map { meta, bam -> [ meta, bam, []] },
+            fasta,
+            fai
+        )
+        ch_versions = ch_versions.mix(SAMTOOLS_CONVERT.out.versions.first())
+        ch_cram_crai = SAMTOOLS_CONVERT.out.cram
+            .join(SAMTOOLS_CONVERT.out.crai, failOnMismatch:true, failOnDuplicate:true)
+    }
+
+    emit:
+    versions      = ch_versions              // channel [ versions ]
+    bam_bai       = ch_bam_bai
+    cram_crai     = ch_cram_crai
+    junctions     = STAR_ALIGN.out.junction
+    spl_junc_tabs = STAR_ALIGN.out.spl_junc_tab
+    log_final     = STAR_ALIGN.out.log_final
+    gene_count    = STAR_ALIGN.out.read_per_gene_tab
+}
+
diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index 938832c96..7d7f511a7 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -1,16 +1,13 @@
 include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION       }   from '../../../modules/nf-core/samtools/index/main'
 include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION_CRAM  }   from '../../../modules/nf-core/samtools/index/main'
 include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_STARFUSION         }   from '../../../modules/nf-core/samtools/view/main'
-include { STAR_ALIGN as STAR_FOR_STARFUSION                     }   from '../../../modules/nf-core/star/align/main'
 include { STARFUSION                                            }   from '../../../modules/local/starfusion/detect/main'
 include { CTATSPLICING_WORKFLOW                                 }   from '../ctatsplicing_workflow'
 
 workflow STARFUSION_WORKFLOW {
     take:
-        reads
-        ch_gtf
-        ch_starindex_ref
-        ch_fasta
+        reads               // channel: [ meta, bam, bai ]
+        junctions           // channel: [ meta, junctions ]
         ch_starfusion_ref
 
     main:
@@ -26,53 +23,21 @@ workflow STARFUSION_WORKFLOW {
                 ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
                                         .map { it -> [ it[0], it[2] ] }
             } else {
-                STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
-                ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
-                ch_align = STAR_FOR_STARFUSION.out.bam_sorted // TODO: This does not seem to be captured and used as the output is bam_sorted_aligned and not bam_sorted
+                reads_junction = reads.join(junctions)  // TODO: This join is not needed as STARFUSION can simply read from the junction file: https://github.com/STAR-Fusion/STAR-Fusion/wiki#alternatively-kickstart-mode-running-star-yourself-and-then-running-star-fusion-using-the-existing-outputs
 
-
-                SAMTOOLS_INDEX_FOR_STARFUSION(STAR_FOR_STARFUSION.out.bam_sorted)
-                ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION.out.versions)
-                bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai)
-                reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )  // TODO: This join is not needed as STARFUSION can simply read from the junction file: https://github.com/STAR-Fusion/STAR-Fusion/wiki#alternatively-kickstart-mode-running-star-yourself-and-then-running-star-fusion-using-the-existing-outputs
-
-                if (params.ctatsplicing || params.all) {
-                    CTATSPLICING_WORKFLOW(
-                        STAR_FOR_STARFUSION.out.spl_junc_tab,
-                        STAR_FOR_STARFUSION.out.junction,
-                        STAR_FOR_STARFUSION.out.bam,
-                        ch_starfusion_ref
-                    )
-                    ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
-                }
-
-                if (params.cram.contains('starfusion')){
-                    SAMTOOLS_VIEW_FOR_STARFUSION (bam_sorted_indexed, ch_fasta, [] )
-                    ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_STARFUSION.out.versions)
-
-                    SAMTOOLS_INDEX_FOR_STARFUSION_CRAM (SAMTOOLS_VIEW_FOR_STARFUSION.out.cram)
-                    ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION_CRAM.out.versions)
-                }
                 if (params.starfusion || params.all){
                     STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
                     ch_versions = ch_versions.mix(STARFUSION.out.versions)
                     ch_starfusion_fusions = STARFUSION.out.fusions
                 }
-
-                ch_star_stats = STAR_FOR_STARFUSION.out.log_final
-                ch_star_gene_count = STAR_FOR_STARFUSION.out.read_per_gene_tab
             }
         }
         else {
             ch_starfusion_fusions = reads.combine(Channel.value(file(ch_dummy_file, checkIfExists:true)))
                                     .map { it -> [ it[0], it[2] ] }
-            ch_star_stats = Channel.empty()
-            ch_star_gene_count = Channel.empty()
         }
     emit:
         fusions               = ch_starfusion_fusions
-        star_stats            = ch_star_stats
-        star_gene_count       = ch_star_gene_count
         ch_bam_sorted         = ch_align
         ch_bam_sorted_indexed = bam_sorted_indexed
         versions              = ch_versions
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ddd006ba3..15c21f38e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -16,8 +16,11 @@ include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusionin
 include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
 include { FASTQC                        }   from '../modules/nf-core/fastqc/main'
 include { MULTIQC                       }   from '../modules/nf-core/multiqc/main'
+include { STAR_ALIGN                    }   from '../modules/nf-core/star/align/main'
 include { SALMON_QUANT                  }   from '../modules/nf-core/salmon/quant/main'
 include { paramsSummaryMap              }   from 'plugin/nf-schema'
+include { FASTQ_ALIGN_STAR              }   from '../subworkflows/local/fastq_align_star'
+include { CTATSPLICING_WORKFLOW         }   from '../subworkflows/local/ctatsplicing_workflow'
 include { paramsSummaryMultiqc          }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
@@ -75,6 +78,44 @@ workflow RNAFUSION {
         ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
         ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
 
+        //
+        // Run STAR alignment
+        //
+
+        def ch_aligned_reads = Channel.empty()
+        def ch_star_junctions = Channel.empty()
+        def ch_star_split_junctions = Channel.empty()
+        if((params.arriba || params.all || params.ctatsplicing || params.starfusion) && !params.fusioninspector_only) {
+            FASTQ_ALIGN_STAR(
+                ch_reads,
+                BUILD_REFERENCES.out.ch_starindex_ref,
+                BUILD_REFERENCES.out.ch_gtf,
+                BUILD_REFERENCES.out.ch_fasta,
+                BUILD_REFERENCES.out.ch_fai,
+                params.star_ignore_sjdbgtf,
+                params.seq_platform,
+                params.seq_center,
+                params.cram
+            )
+            ch_versions = ch_versions.mix(FASTQ_ALIGN_STAR.out.versions)
+            ch_aligned_reads = FASTQ_ALIGN_STAR.out.bam_bai
+            ch_star_junctions = FASTQ_ALIGN_STAR.out.junctions
+            ch_star_split_junctions = FASTQ_ALIGN_STAR.out.spl_junc_tabs
+        }
+
+        //
+        // Run CTAT-SPLICING
+        //
+
+        if((params.ctatsplicing || params.all)) {
+            CTATSPLICING_WORKFLOW(
+                ch_star_split_junctions,
+                ch_star_junctions,
+                ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
+                BUILD_REFERENCES.out.ch_starfusion_ref
+            )
+            ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
+        }
 
         //
         // SUBWORKFLOW:  Run STAR alignment and Arriba
@@ -85,49 +126,39 @@ workflow RNAFUSION {
         // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
 
         ARRIBA_WORKFLOW (
-            ch_reads,
+            ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
             BUILD_REFERENCES.out.ch_gtf,
             BUILD_REFERENCES.out.ch_fasta,
-            BUILD_REFERENCES.out.ch_starindex_ref,
             BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
             BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
             BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
             BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
-            BUILD_REFERENCES.out.ch_starfusion_ref,
             params.arriba,                   // boolean
             params.all,                      // boolean
             params.fusioninspector_only,     // boolean
-            params.star_ignore_sjdbgtf,      // boolean
-            params.ctatsplicing,             // boolean
-            params.seq_center ?: '',         // string
             params.arriba_fusions,           // path
-            params.cram                      // array
         )
         ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
 
 
         //Run STAR fusion
         STARFUSION_WORKFLOW (
-            ch_reads,
-            BUILD_REFERENCES.out.ch_gtf,
-            BUILD_REFERENCES.out.ch_starindex_ref,
-            BUILD_REFERENCES.out.ch_fasta,
+            ch_aligned_reads,
+            ch_star_junctions,
             BUILD_REFERENCES.out.ch_starfusion_ref
         )
         ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
 
-
-
-    //Run fusioncatcher
-    FUSIONCATCHER_WORKFLOW (
-        ch_reads,
-        BUILD_REFERENCES.out.ch_fusioncatcher_ref,       // channel [ meta, path       ]
-        params.run_fusioncatcher,
-        params.all,
-        params.fusioninspector_only,
-        params.fusioncatcher_fusions
-    )
-    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+        //Run fusioncatcher
+        FUSIONCATCHER_WORKFLOW (
+            ch_reads,
+            BUILD_REFERENCES.out.ch_fusioncatcher_ref,       // channel [ meta, path       ]
+            params.run_fusioncatcher,
+            params.all,
+            params.fusioninspector_only,
+            params.fusioncatcher_fusions
+        )
+        ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
 
 
         //Run stringtie
@@ -224,8 +255,8 @@ workflow RNAFUSION {
         ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.log_final.collect{it[1]}.ifEmpty([]))
+        ch_multiqc_files                      = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.gene_count.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
         ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))

From 9ad81f21cbc01e1cf540a5c296d784f6a8e6970d Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 17 Mar 2025 16:54:32 +0100
Subject: [PATCH 339/887] small input tuple mismatch fix

---
 subworkflows/local/starfusion_workflow/main.nf | 2 +-
 workflows/rnafusion.nf                         | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index 7d7f511a7..705cb3257 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -6,7 +6,7 @@ include { CTATSPLICING_WORKFLOW                                 }   from '../cta
 
 workflow STARFUSION_WORKFLOW {
     take:
-        reads               // channel: [ meta, bam, bai ]
+        reads               // channel: [ meta, bam ]
         junctions           // channel: [ meta, junctions ]
         ch_starfusion_ref
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 15c21f38e..2cce2a64d 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -143,7 +143,7 @@ workflow RNAFUSION {
 
         //Run STAR fusion
         STARFUSION_WORKFLOW (
-            ch_aligned_reads,
+            ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
             ch_star_junctions,
             BUILD_REFERENCES.out.ch_starfusion_ref
         )

From 1ce77b98a5725864f36ccbdcca3f19eb7e01ecd3 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 17 Mar 2025 17:15:19 +0100
Subject: [PATCH 340/887] fix samtools convert stub + use aligned bam

---
 conf/modules.config                                    |  2 +-
 modules.json                                           |  2 +-
 modules/nf-core/samtools/convert/main.nf               |  2 +-
 .../nf-core/samtools/convert/tests/main.nf.test.snap   | 10 +++++-----
 subworkflows/local/fastq_align_star/main.nf            |  6 +++---
 5 files changed, 11 insertions(+), 11 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 68e2e3478..30247beae 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -228,7 +228,7 @@ process {
     withName: 'SAMTOOLS_CONVERT' {
         ext.prefix = { "${meta.id}.Aligned.sortedByCoord.out" }
         publishDir = [
-            path: { "${params.outdir}/cram" },
+            path: { "${params.outdir}/star" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
diff --git a/modules.json b/modules.json
index 79e06b4e9..2b54916e4 100644
--- a/modules.json
+++ b/modules.json
@@ -98,7 +98,7 @@
                     },
                     "samtools/convert": {
                         "branch": "master",
-                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
+                        "git_sha": "b823b7220bac94079f9ea65113401956ca7d633e",
                         "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
diff --git a/modules/nf-core/samtools/convert/main.nf b/modules/nf-core/samtools/convert/main.nf
index cf9253d10..9667e72d8 100644
--- a/modules/nf-core/samtools/convert/main.nf
+++ b/modules/nf-core/samtools/convert/main.nf
@@ -50,7 +50,7 @@ process SAMTOOLS_CONVERT {
 
     """
     touch ${prefix}.${output_extension}
-    touch ${prefix}.${index_extension}
+    touch ${prefix}.${output_extension}.${index_extension}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test.snap b/modules/nf-core/samtools/convert/tests/main.nf.test.snap
index a021254e8..a7bff30d5 100644
--- a/modules/nf-core/samtools/convert/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/convert/tests/main.nf.test.snap
@@ -67,7 +67,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "4": [
@@ -85,7 +85,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "cram": [
@@ -103,10 +103,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-09-16T07:52:45.799885099"
+        "timestamp": "2025-03-17T17:05:45.33625491"
     },
     "bam_to_cram_index": {
         "content": [
diff --git a/subworkflows/local/fastq_align_star/main.nf b/subworkflows/local/fastq_align_star/main.nf
index 2477b31bd..194c0c29c 100644
--- a/subworkflows/local/fastq_align_star/main.nf
+++ b/subworkflows/local/fastq_align_star/main.nf
@@ -27,17 +27,17 @@ workflow FASTQ_ALIGN_STAR {
     ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first())
 
     SAMTOOLS_INDEX(
-        STAR_ALIGN.out.bam_sorted
+        STAR_ALIGN.out.bam_sorted_aligned
     )
     ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first())
 
-    ch_bam_bai = STAR_ALIGN.out.bam_sorted
+    ch_bam_bai = STAR_ALIGN.out.bam_sorted_aligned
         .join(SAMTOOLS_INDEX.out.bai, failOnMismatch:true, failOnDuplicate:true)
 
     def ch_cram_crai = Channel.empty()
     if(cram) {
         SAMTOOLS_CONVERT(
-            STAR_ALIGN.out.bam_sorted.map { meta, bam -> [ meta, bam, []] },
+            STAR_ALIGN.out.bam_sorted_aligned.map { meta, bam -> [ meta, bam, []] },
             fasta,
             fai
         )

From ab7acbdce87f1a12acaea76bb6de239033673fdc Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 17 Mar 2025 17:38:03 +0100
Subject: [PATCH 341/887] correct bam flow in rnafusion.nf

---
 workflows/rnafusion.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2cce2a64d..4168c3cbb 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -163,7 +163,7 @@ workflow RNAFUSION {
 
         //Run stringtie
         STRINGTIE_WORKFLOW (
-            STARFUSION_WORKFLOW.out.ch_bam_sorted,
+            ch_aligned_reads.map { meta, bam, _bai -> [meta, bam]},
             BUILD_REFERENCES.out.ch_gtf
         )
         ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
@@ -198,7 +198,7 @@ workflow RNAFUSION {
 
         //QC
         QC_WORKFLOW (
-            STARFUSION_WORKFLOW.out.ch_bam_sorted,
+            ch_aligned_reads,
             BUILD_REFERENCES.out.ch_refflat,
             BUILD_REFERENCES.out.ch_fasta,
             BUILD_REFERENCES.out.ch_fai,

From c386899914392097915412a3b34f27bf4c723d4b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 17 Mar 2025 17:43:32 +0100
Subject: [PATCH 342/887] fix test

---
 tests/test_stub.nf.test.snap | 216 ++++++++++++-----------------------
 1 file changed, 72 insertions(+), 144 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 3a4257485..c19d56a5f 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,7 +1,7 @@
 {
     "stub test no fastp trim": {
         "content": [
-            31,
+            29,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -67,7 +67,7 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
                 "STARFUSION": {
@@ -76,14 +76,9 @@
                 "STARFUSION_BUILD": {
                     "STAR-Fusion": "1.14.0"
                 },
-                "STAR_FOR_ARRIBA": {
-                    "star": "2.7.11b",
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "STAR_FOR_STARFUSION": {
+                "STAR_ALIGN": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -106,30 +101,17 @@
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
                 "ctatsplicing",
-                "ctatsplicing/arriba",
-                "ctatsplicing/arriba/test.cancer.introns",
-                "ctatsplicing/arriba/test.cancer.introns.prelim",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/arriba/test.chckpts",
-                "ctatsplicing/arriba/test.ctat-splicing.igv.html",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/arriba/test.igv.tracks",
-                "ctatsplicing/arriba/test.introns",
-                "ctatsplicing/arriba/test.introns.for_IGV.bed",
-                "ctatsplicing/starfusion",
-                "ctatsplicing/starfusion/test.cancer.introns",
-                "ctatsplicing/starfusion/test.cancer.introns.prelim",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/starfusion/test.chckpts",
-                "ctatsplicing/starfusion/test.ctat-splicing.igv.html",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/starfusion/test.igv.tracks",
-                "ctatsplicing/starfusion/test.introns",
-                "ctatsplicing/starfusion/test.introns.for_IGV.bed",
+                "ctatsplicing/test.cancer.introns",
+                "ctatsplicing/test.cancer.introns.prelim",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/test.chckpts",
+                "ctatsplicing/test.ctat-splicing.igv.html",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/test.igv.tracks",
+                "ctatsplicing/test.introns",
+                "ctatsplicing/test.introns.for_IGV.bed",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -228,43 +210,25 @@
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
                 "salmon/test_meta_info.json",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
                 "starfusion",
                 "starfusion/ctat_genome_lib_build_dir",
                 "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
@@ -355,13 +319,13 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-01-07T13:16:02.754052"
+        "timestamp": "2025-03-17T17:40:40.562156411"
     },
     "stub test with fastp trim": {
         "content": [
-            33,
+            31,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -433,7 +397,7 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
                 "STARFUSION": {
@@ -442,14 +406,9 @@
                 "STARFUSION_BUILD": {
                     "STAR-Fusion": "1.14.0"
                 },
-                "STAR_FOR_ARRIBA": {
-                    "star": "2.7.11b",
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "STAR_FOR_STARFUSION": {
+                "STAR_ALIGN": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -472,30 +431,17 @@
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
                 "ctatsplicing",
-                "ctatsplicing/arriba",
-                "ctatsplicing/arriba/test.cancer.introns",
-                "ctatsplicing/arriba/test.cancer.introns.prelim",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/arriba/test.chckpts",
-                "ctatsplicing/arriba/test.ctat-splicing.igv.html",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/arriba/test.igv.tracks",
-                "ctatsplicing/arriba/test.introns",
-                "ctatsplicing/arriba/test.introns.for_IGV.bed",
-                "ctatsplicing/starfusion",
-                "ctatsplicing/starfusion/test.cancer.introns",
-                "ctatsplicing/starfusion/test.cancer.introns.prelim",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/starfusion/test.chckpts",
-                "ctatsplicing/starfusion/test.ctat-splicing.igv.html",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/starfusion/test.igv.tracks",
-                "ctatsplicing/starfusion/test.introns",
-                "ctatsplicing/starfusion/test.introns.for_IGV.bed",
+                "ctatsplicing/test.cancer.introns",
+                "ctatsplicing/test.cancer.introns.prelim",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/test.chckpts",
+                "ctatsplicing/test.ctat-splicing.igv.html",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/test.igv.tracks",
+                "ctatsplicing/test.introns",
+                "ctatsplicing/test.introns.for_IGV.bed",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -603,43 +549,25 @@
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
                 "salmon/test_meta_info.json",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
                 "starfusion",
                 "starfusion/ctat_genome_lib_build_dir",
                 "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
@@ -730,8 +658,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-01-07T13:13:38.690939"
+        "timestamp": "2025-03-17T17:38:45.56378377"
     }
 }
\ No newline at end of file

From 92017377adeb7cb95509e36fc177ca96bf11cfd8 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 18 Mar 2025 10:04:30 +0100
Subject: [PATCH 343/887] update changelog

---
 CHANGELOG.md           | 4 +++-
 workflows/rnafusion.nf | 3 +--
 2 files changed, 4 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fff397252..2d24f8d7c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -47,7 +47,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update`FUSIONINSPECTOR` to v2.10.0. [#601](https://github.com/nf-core/rnafusion/pull/601)
 - Remove local module `STARFUSION_DOWNLOAD` [#598](https://github.com/nf-core/rnafusion/pull/598)
 - Fix error message when parameter outdir is missing [#611](https://github.com/nf-core/rnafusion/pull/611)
-- Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
+- Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between thttps://github.com/nf-core/rnafusion/pull/633ool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
+- The STAR alignment now only runs once instead of multiple times when using `--arriba` and `--starfusion` [#633](https://github.com/nf-core/rnafusion/pull/633)
+- The `--cram` parameter has been converted to a boolean value instead of the comma-separated list of values. Use this parameter if you also want to create CRAM files from the BAM files created with STAR [#633](https://github.com/nf-core/rnafusion/pull/633)
 
 ### Fixed
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 4168c3cbb..9e2c1ff8f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -85,7 +85,7 @@ workflow RNAFUSION {
         def ch_aligned_reads = Channel.empty()
         def ch_star_junctions = Channel.empty()
         def ch_star_split_junctions = Channel.empty()
-        if((params.arriba || params.all || params.ctatsplicing || params.starfusion) && !params.fusioninspector_only) {
+        if((params.arriba || params.ctatsplicing || params.starfusion || params.all) && !params.fusioninspector_only) {
             FASTQ_ALIGN_STAR(
                 ch_reads,
                 BUILD_REFERENCES.out.ch_starindex_ref,
@@ -121,7 +121,6 @@ workflow RNAFUSION {
         // SUBWORKFLOW:  Run STAR alignment and Arriba
         //
 
-        // TODO: add params.seq_platform and pass it as argument to arriba_workflow
         // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
         // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
 

From 0b1d31f1029efd2f384e482b5553dca1c57124e9 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 18 Mar 2025 17:18:26 +0100
Subject: [PATCH 344/887] fix language server errors

---
 modules.json                                  |  6 +-
 modules/nf-core/arriba/arriba/main.nf         | 16 ++--
 modules/nf-core/salmon/quant/environment.yml  |  2 +
 modules/nf-core/salmon/quant/main.nf          |  3 +-
 modules/nf-core/star/align/environment.yml    |  6 +-
 modules/nf-core/star/align/main.nf            | 13 +--
 .../star/align/tests/main.nf.test.snap        | 90 +++++++++----------
 .../local/fusioncatcher_workflow/main.nf      |  4 +-
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  2 -
 9 files changed, 73 insertions(+), 69 deletions(-)

diff --git a/modules.json b/modules.json
index 01e049b64..bc3fe7962 100644
--- a/modules.json
+++ b/modules.json
@@ -12,7 +12,7 @@
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "7741dfc830e77a8ead2fcb50b01461ee09d0cdfe",
+                        "git_sha": "1719e20e3881b37b9e1e8498ea669a2b92e54b9a",
                         "installed_by": ["modules"]
                     },
                     "arriba/download": {
@@ -93,7 +93,7 @@
                     },
                     "salmon/quant": {
                         "branch": "master",
-                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
+                        "git_sha": "85b5f8a0d9df9ce7587af50e2ee75b37c97515c6",
                         "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
@@ -118,7 +118,7 @@
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "a5ad53288c79fa52c5ae708c317e09ec2dd149ab",
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
                         "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
diff --git a/modules/nf-core/arriba/arriba/main.nf b/modules/nf-core/arriba/arriba/main.nf
index 75dbf93ad..b89231280 100644
--- a/modules/nf-core/arriba/arriba/main.nf
+++ b/modules/nf-core/arriba/arriba/main.nf
@@ -27,10 +27,10 @@ process ARRIBA_ARRIBA {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def blacklist = blacklist ? "-b $blacklist" : "-f blacklist"
-    def known_fusions = known_fusions ? "-k $known_fusions" : ""
-    def cytobands = cytobands ? "-d $cytobands" : ""
-    def protein_domains = protein_domains ? "-p $protein_domains" : ""
+    def blacklist_arg = blacklist ? "-b $blacklist" : "-f blacklist"
+    def known_fusions_arg = known_fusions ? "-k $known_fusions" : ""
+    def cytobands_arg = cytobands ? "-d $cytobands" : ""
+    def protein_domains_arg = protein_domains ? "-p $protein_domains" : ""
 
     """
     arriba \\
@@ -39,10 +39,10 @@ process ARRIBA_ARRIBA {
         -g $gtf \\
         -o ${prefix}.fusions.tsv \\
         -O ${prefix}.fusions.discarded.tsv \\
-        $blacklist \\
-        $known_fusions \\
-        $cytobands \\
-        $protein_domains \\
+        $blacklist_arg \\
+        $known_fusions_arg \\
+        $cytobands_arg \\
+        $protein_domains_arg \\
         $args
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/salmon/quant/environment.yml b/modules/nf-core/salmon/quant/environment.yml
index b3f75777e..c9d531434 100644
--- a/modules/nf-core/salmon/quant/environment.yml
+++ b/modules/nf-core/salmon/quant/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index f1e3b5cda..e2d273525 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -29,7 +29,8 @@ process SALMON_QUANT {
     prefix   = task.ext.prefix ?: "${meta.id}"
 
     def reference   = "--index $index"
-    def reads1 = [], reads2 = []
+    def reads1 = []
+    def reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def input_reads = meta.single_end ? "-r ${reads1.join(" ")}" : "-1 ${reads1.join(" ")} -2 ${reads2.join(" ")}"
     if (alignment_mode) {
diff --git a/modules/nf-core/star/align/environment.yml b/modules/nf-core/star/align/environment.yml
index 7c57530a3..91e37ae12 100644
--- a/modules/nf-core/star/align/environment.yml
+++ b/modules/nf-core/star/align/environment.yml
@@ -1,9 +1,11 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 
 dependencies:
-  - bioconda::htslib=1.20
-  - bioconda::samtools=1.20
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
   - bioconda::star=2.7.11b
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/align/main.nf b/modules/nf-core/star/align/main.nf
index 417071ba9..22a52184d 100644
--- a/modules/nf-core/star/align/main.nf
+++ b/modules/nf-core/star/align/main.nf
@@ -4,8 +4,8 @@ process STAR_ALIGN {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b4/b425bc2a95806d878993f9a66dae3ae80ac2dafff4c208c5ae01b7a90a32fa91/data' :
-        'community.wave.seqera.io/library/star_samtools_htslib_gawk:10c6e8c834460019' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/26/268b4c9c6cbf8fa6606c9b7fd4fafce18bf2c931d1a809a0ce51b105ec06c89d/data' :
+        'community.wave.seqera.io/library/htslib_samtools_star_gawk:ae438e9a604351a4' }"
 
     input:
     tuple val(meta), path(reads, stageAs: "input*/*")
@@ -41,12 +41,13 @@ process STAR_ALIGN {
     script:
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
-    def reads1 = [], reads2 = []
+    def reads1 = []
+    def reads2 = []
     meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
     def ignore_gtf      = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf"
-    def seq_platform    = seq_platform ? "'PL:$seq_platform'" : ""
-    def seq_center      = seq_center ? "'CN:$seq_center'" : ""
-    attrRG          = args.contains("--outSAMattrRGline") ? "" : "--outSAMattrRGline 'ID:$prefix' $seq_center 'SM:$prefix' $seq_platform"
+    def seq_platform_arg  = seq_platform ? "'PL:$seq_platform'" : ""
+    def seq_center_arg    = seq_center ? "'CN:$seq_center'" : ""
+    attrRG          = args.contains("--outSAMattrRGline") ? "" : "--outSAMattrRGline 'ID:$prefix' $seq_center_arg 'SM:$prefix' $seq_platform_arg"
     def out_sam_type    = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted'
     mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : ''
     """
diff --git a/modules/nf-core/star/align/tests/main.nf.test.snap b/modules/nf-core/star/align/tests/main.nf.test.snap
index b533fb8b4..a1ec3a3d0 100644
--- a/modules/nf-core/star/align/tests/main.nf.test.snap
+++ b/modules/nf-core/star/align/tests/main.nf.test.snap
@@ -97,7 +97,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -307,7 +307,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -321,10 +321,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:08.738074176"
+        "timestamp": "2024-12-11T16:28:39.242074494"
     },
     "homo_sapiens - paired_end - arriba - stub": {
         "content": [
@@ -424,7 +424,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -634,7 +634,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -648,10 +648,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:36.122131869"
+        "timestamp": "2024-12-11T16:29:08.392620556"
     },
     "homo_sapiens - single_end": {
         "content": [
@@ -703,14 +703,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:01:22.197991909"
+        "timestamp": "2024-12-11T16:21:34.549483887"
     },
     "homo_sapiens - paired_end": {
         "content": [
@@ -762,14 +762,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:02:06.988663857"
+        "timestamp": "2024-12-11T16:22:17.011253146"
     },
     "homo_sapiens - paired_end - multiple - stub": {
         "content": [
@@ -869,7 +869,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -1079,7 +1079,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -1093,10 +1093,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:10:12.005468781"
+        "timestamp": "2024-12-11T16:29:31.072104862"
     },
     "homo_sapiens - paired_end - multiple": {
         "content": [
@@ -1148,14 +1148,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:08:54.877286681"
+        "timestamp": "2024-12-11T16:28:26.645008336"
     },
     "homo_sapiens - paired_end - stub": {
         "content": [
@@ -1255,7 +1255,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -1465,7 +1465,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -1479,10 +1479,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:20.911466345"
+        "timestamp": "2024-12-11T16:28:53.816280805"
     },
     "homo_sapiens - paired_end - starfusion": {
         "content": [
@@ -1525,14 +1525,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:07:25.0639914"
+        "timestamp": "2024-12-11T16:27:14.136111964"
     },
     "homo_sapiens - paired_end - arriba": {
         "content": [
@@ -1574,14 +1574,14 @@
                 
             ],
             [
-                "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:04:00.685784211"
+        "timestamp": "2024-12-11T16:24:00.829462526"
     },
     "homo_sapiens - paired_end - starfusion - stub": {
         "content": [
@@ -1681,7 +1681,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "4": [
                     [
@@ -1891,7 +1891,7 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a149bba1dbb5194560abdd813c7848e3"
+                    "versions.yml:md5,b416145d7b5b8a080e832a7f7cde4c44"
                 ],
                 "wig": [
                     [
@@ -1905,9 +1905,9 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-20T17:09:53.173671551"
+        "timestamp": "2024-12-11T16:29:20.015372487"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index fd9ce34b9..b03908e35 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -22,7 +22,7 @@ workflow FUSIONCATCHER_WORKFLOW {
             if (fusioncatcher_fusions){
 
                 ch_fusioncatcher_fusions = reads.combine(Channel.value(file(fusioncatcher_fusions, checkIfExists:true)))
-                                            .map { meta, reads, fusions -> [ meta, fusions ] }
+                                            .map { meta, _reads, fusions -> [ meta, fusions ] }
             } else {
 
                 FUSIONCATCHER_DETECT (
@@ -35,7 +35,7 @@ workflow FUSIONCATCHER_WORKFLOW {
         }
         else {
             ch_fusioncatcher_fusions = reads.combine(Channel.value(file(ch_dummy_file, checkIfExists:true)))
-                                        .map { meta, reads, fusions -> [ meta, fusions ] }
+                                        .map { meta, _reads, fusions -> [ meta, fusions ] }
         }
 
     emit:
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 7e0362286..378b89f1b 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -2,8 +2,6 @@
 // Subworkflow with functionality specific to the nf-core/rnafusion pipeline
 //
 
-import groovy.json.JsonSlurper
-
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS

From 8e998c561043c2b75c9e3aba40a581bedd5748a8 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 18 Mar 2025 17:19:41 +0100
Subject: [PATCH 345/887] changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index fff397252..aab21894d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -59,6 +59,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed bug in `GFFREAD` that caused output `gffread_fasta` not being produced [#565](https://github.com/nf-core/rnafusion/issues/565)
 - Fixed bug in `FUSIONCATCHER_DOWNLOAD` that caused an error when running with singularity profile [#573](https://github.com/nf-core/rnafusion/issues/573)
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
+- Fixed the codebase to be compatible with the Nextflow language server [#634](https://github.com/nf-core/rnafusion/pull/634)
 
 ### Removed
 

From ed6c8bff68ff237cee6619d96e3f984c2a1ff80d Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 18 Mar 2025 17:36:30 +0100
Subject: [PATCH 346/887] mention version bump

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index aab21894d..a10feb0b6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -48,6 +48,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Remove local module `STARFUSION_DOWNLOAD` [#598](https://github.com/nf-core/rnafusion/pull/598)
 - Fix error message when parameter outdir is missing [#611](https://github.com/nf-core/rnafusion/pull/611)
 - Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
+- Update htslib and samtools version in `star/align` to 1.21 [#634](https://github.com/nf-core/rnafusion/pull/634)
 
 ### Fixed
 

From 94049a66fcc8b2594550d7a51ef0d647e4650df1 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 18 Mar 2025 16:53:43 +0000
Subject: [PATCH 347/887] add tests for prepgenomelib (TODO snapshot tomorrow)

---
 .../local/ctatsplicing/prepgenomelib/main.nf  |  2 +-
 .../prepgenomelib/tests/main.nf.test          | 77 +++++++++++++++++++
 2 files changed, 78 insertions(+), 1 deletion(-)
 create mode 100644 modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test

diff --git a/modules/local/ctatsplicing/prepgenomelib/main.nf b/modules/local/ctatsplicing/prepgenomelib/main.nf
index 0fbf5e9e4..04f09e1aa 100644
--- a/modules/local/ctatsplicing/prepgenomelib/main.nf
+++ b/modules/local/ctatsplicing/prepgenomelib/main.nf
@@ -31,7 +31,7 @@ process CTATSPLICING_PREPGENOMELIB {
     def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
     touch $genome_lib/refGene.bed
-    touch $genome_lib/refGene.sort.bed.gz
+    echo | gzip > $genome_lib/refGene.sort.bed.gz
     touch $genome_lib/refGene.sort.bed.gz.tbi
     mkdir $genome_lib/cancer_splicing_lib
     touch $genome_lib/cancer_splicing_lib/cancer_splicing.idx
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
new file mode 100644
index 000000000..de69cd3e2
--- /dev/null
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -0,0 +1,77 @@
+nextflow_process {
+
+    name "Test Process CTATSPLICING_PREPGENOMELIB"
+    script "../main.nf"
+    process "CTATSPLICING_PREPGENOMELIB"
+
+    setup {
+        run("STARFUSION_BUILD") {
+            script "../../../starfusion/build/main.nf"
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                ]
+                input[1] = [
+                    [ id:'minigenome gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                ]
+
+                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input [3] = "human"
+                """
+            }
+        }
+    }
+
+    test("test") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = STARFUSION_BUILD.out.reference
+                input[1] = [
+                    file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
+            )
+        }
+    }
+
+    test("test - stub") {
+
+        options "-stub"
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = STARFUSION_BUILD.out.reference
+                input[1] = [
+                    file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
+            )
+        }
+    }
+}

From 76926ffead9cd9e166b57c02703080f083b84985 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 19 Mar 2025 10:07:01 +0100
Subject: [PATCH 348/887] bump snap

---
 tests/test_stub.nf.test.snap | 16 ++++++++--------
 1 file changed, 8 insertions(+), 8 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 3a4257485..e78ec8de1 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -78,12 +78,12 @@
                 },
                 "STAR_FOR_ARRIBA": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -355,9 +355,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-01-07T13:16:02.754052"
+        "timestamp": "2025-03-19T09:52:40.993862676"
     },
     "stub test with fastp trim": {
         "content": [
@@ -444,12 +444,12 @@
                 },
                 "STAR_FOR_ARRIBA": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STAR_FOR_STARFUSION": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -730,8 +730,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-01-07T13:13:38.690939"
+        "timestamp": "2025-03-19T09:51:49.095272949"
     }
 }
\ No newline at end of file

From 013773cdbd340fe8e45e2e3570c25aea0ae0f161 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 19 Mar 2025 10:54:46 +0000
Subject: [PATCH 349/887] add prepgenome lib snap

---
 conf/modules.config                           |   1 -
 .../prepgenomelib/tests/main.nf.test.snap     | 212 ++++++++++++++++++
 2 files changed, 212 insertions(+), 1 deletion(-)
 create mode 100644 modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap

diff --git a/conf/modules.config b/conf/modules.config
index c13cb3d2f..0fc0be935 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -370,7 +370,6 @@ process {
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
-        ext.args = "--max_readlength ${params.read_length} --human_gencode_filter"
     }
 
     withName: 'CTATSPLICING_PREPGENOMELIB' {
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
new file mode 100644
index 000000000..b0fa56d6d
--- /dev/null
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
@@ -0,0 +1,212 @@
+{
+    "test": {
+        "content": [
+            {
+                "reference": [
+                    [
+                        {
+                            "id": "minigenome fasta"
+                        },
+                        [
+                            "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+                            "PFAM.domtblout.dat.gz:md5,26c8d24b6cd736eef6b5d2ddaf337672",
+                            [
+                                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
+                            "blast_pairs.dat.gz:md5,3e7fe3a6fa372742b8574940abdc9a37",
+                            "blast_pairs.idx:md5,b8a9567c9f2b8f099a95956e7dadfdd5",
+                            [
+                                "cancer_splicing.idx:md5,712867939a7c235bdc47dd3fdb03e014"
+                            ],
+                            "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                            "fusion_annot_lib.idx:md5,d77513b8a65344e32f976cb9df54e0c4",
+                            "pfam_domains.dbm:md5,03f514fe96a42d54eef7429eb7bd2216",
+                            "refGene.bed:md5,d854402131f44d84a14cd90f912f886d",
+                            "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
+                            "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
+                            "ref_annot.cdna.fa:md5,a912c9684f1f14b266ff8f6ee6b16b6c",
+                            "ref_annot.cdna.fa.idx:md5,8555d23ba563a84c34a55e9a7b1b14c9",
+                            "ref_annot.cds:md5,19c35508510e3cbd13d70e586712ff71",
+                            "ref_annot.cdsplus.fa:md5,cee60e0eed67964731618f2cb39eb509",
+                            "ref_annot.cdsplus.fa.idx:md5,555823919c78863b03505ed793299562",
+                            "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                            "ref_annot.gtf.gene_spans:md5,20f0a837edf800511c65436f9f5372b6",
+                            "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+                            "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                            "ref_annot.pep:md5,655d2d2955149bf632e7ba7036bbc01c",
+                            "ref_annot.prot_info.dbm:md5,6298fed0de42c97c668e3fb8ac4d5e20",
+                            "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+                            "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                            "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+                            "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+                            "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                            "ref_genome.fa.nin:md5,2317a90875fe530dff8e2b95bc8bbee1",
+                            "ref_genome.fa.njs:md5,3127dd8e9e5897924974328a359e383d",
+                            "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+                            "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+                            "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+                            "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+                            [
+                                "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                                "Log.out:md5,f0d53c14008b8a936d65a458ac01345f",
+                                "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+                                "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+                                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                                "genomeParameters.txt:md5,767f208cbd11c22bcb315b0254b0d9de",
+                                "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+                                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2"
+                            ],
+                            "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                            "trans.blast.align_coords.align_coords.dbm:md5,02f34eb24b0888e0d6adafd4860fafb9",
+                            "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,8175cd19f5d5b8b2214cd948120d7524"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-19T10:53:52.549180977"
+    },
+    "test - stub": {
+        "content": [
+            {
+                "reference": [
+                    [
+                        {
+                            "id": "minigenome fasta"
+                        },
+                        [
+                            "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
+                            "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "cancer_splicing.idx:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
+                            "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "refGene.sort.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "refGene.sort.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            [
+                                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            ],
+                            "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,8175cd19f5d5b8b2214cd948120d7524"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-19T09:17:05.045966772"
+    }
+}
\ No newline at end of file

From 0f2657880cb8647249358fabe11126e684b697a8 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 19 Mar 2025 13:15:20 +0000
Subject: [PATCH 350/887] fix test_build snap

---
 tests/test_build.nf.test.snap | 174 +++++++++++++++++++---------------
 1 file changed, 96 insertions(+), 78 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 30d80c733..8f8601629 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -107,82 +107,82 @@
                 "references/star/sjdbList.fromGTF.out.tab",
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
-                "starfusion",
-                "starfusion/ctat_genome_lib_build_dir",
-                "starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz"
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz"
             ],
             [
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
@@ -220,6 +220,7 @@
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+                "PFAM.domtblout.dat.gz:md5,c828178dba2ddd7266060754e4b2adbb",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -243,20 +244,35 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.dat.gz:md5,c9dff0590ddd39631bd06a2e3b29bae2",
+                "blast_pairs.idx:md5,f3def0be5240e370fe202363467c1551",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                "fusion_annot_lib.idx:md5,0b302029cafb22a7d24c8be6d2075645",
+                "pfam_domains.dbm:md5,47050a6635345fc6b79e80b1d603bc02",
+                "ref_annot.cdna.fa:md5,ccb172e35cc5696bbc24cdfa33129f77",
+                "ref_annot.cdna.fa.idx:md5,eeb7bdd0ea9d26278448a27e1a3ec8ea",
+                "ref_annot.cds:md5,48fb7043d6ca82fb6dfd737980e5c712",
+                "ref_annot.cdsplus.fa:md5,d004f2c68707a5a5309ff90e168fad17",
+                "ref_annot.cdsplus.fa.idx:md5,9e8eea63b03ded2d8efc43bd57963d10",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                "ref_annot.gtf.gene_spans:md5,04bc2543d99d7a0be4f87a733dc0798d",
                 "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                 "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                "ref_annot.pep:md5,8f1efe2a1b7288a886609e341ad7c441",
+                "ref_annot.prot_info.dbm:md5,3a7c8492fe5d0214bb7e01cf1740af8d",
                 "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                 "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                 "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                 "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                "ref_genome.fa.nin:md5,f7e84e3ed403933bc0cb7d528619e94d",
+                "ref_genome.fa.njs:md5,f51ce88d639b0c3142a309dbf0c1e41c",
                 "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                 "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                 "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                 "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                "Log.out:md5,23f80ce482ce4779ff88c129e4b6a6e5",
                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -267,18 +283,20 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "genomeParameters.txt:md5,f9e87dbcaa7bc8ea9df576ba3ed3bbd0",
                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                "trans.blast.align_coords.align_coords.dbm:md5,a15f136b73f31fd474450e70e7e85e7b",
                 "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-01-14T13:08:24.564679638"
+        "timestamp": "2025-03-19T13:09:57.878292804"
     }
 }
\ No newline at end of file

From 4618f19afc8265f551f386b6d40d0cb57f6af5be Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 19 Mar 2025 14:53:47 +0000
Subject: [PATCH 351/887] ignore unstable files

---
 tests/.nftignore              | 38 +++++++++++++++++------------------
 tests/test_build.nf.test.snap | 20 +-----------------
 2 files changed, 20 insertions(+), 38 deletions(-)

diff --git a/tests/.nftignore b/tests/.nftignore
index cb651aedf..3bba86da0 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -13,25 +13,25 @@ references/salmon/salmon/pre_indexing.log
 references/salmon/salmon/ref_indexing.log
 references/salmon/salmon/seq.bin
 references/star/Log.out
-starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/
-starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
-starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz
-starfusion/ctat_genome_lib_build_dir/blast_pairs.idx
-starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx
-starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm
-starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa
-starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx
-starfusion/ctat_genome_lib_build_dir/ref_annot.cds
-starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
-starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
-starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
-starfusion/ctat_genome_lib_build_dir/ref_annot.pep
-starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
-starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin
-starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs
-starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
-starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
+references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/
+references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
+references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz
+references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx
+references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx
+references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep
+references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
+references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin
+references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs
+references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
+references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
 references/star/genomeParameters.txt
-starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
+references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
 references/fusion_report_db/mitelman.db
 references/fusion_report_db/fusiongdb2.db
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 8f8601629..2d07ed660 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -220,7 +220,6 @@
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
-                "PFAM.domtblout.dat.gz:md5,c828178dba2ddd7266060754e4b2adbb",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -244,35 +243,20 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.dat.gz:md5,c9dff0590ddd39631bd06a2e3b29bae2",
-                "blast_pairs.idx:md5,f3def0be5240e370fe202363467c1551",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                "fusion_annot_lib.idx:md5,0b302029cafb22a7d24c8be6d2075645",
-                "pfam_domains.dbm:md5,47050a6635345fc6b79e80b1d603bc02",
-                "ref_annot.cdna.fa:md5,ccb172e35cc5696bbc24cdfa33129f77",
-                "ref_annot.cdna.fa.idx:md5,eeb7bdd0ea9d26278448a27e1a3ec8ea",
-                "ref_annot.cds:md5,48fb7043d6ca82fb6dfd737980e5c712",
-                "ref_annot.cdsplus.fa:md5,d004f2c68707a5a5309ff90e168fad17",
-                "ref_annot.cdsplus.fa.idx:md5,9e8eea63b03ded2d8efc43bd57963d10",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                "ref_annot.gtf.gene_spans:md5,04bc2543d99d7a0be4f87a733dc0798d",
                 "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                 "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                "ref_annot.pep:md5,8f1efe2a1b7288a886609e341ad7c441",
-                "ref_annot.prot_info.dbm:md5,3a7c8492fe5d0214bb7e01cf1740af8d",
                 "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                 "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                 "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                 "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                "ref_genome.fa.nin:md5,f7e84e3ed403933bc0cb7d528619e94d",
-                "ref_genome.fa.njs:md5,f51ce88d639b0c3142a309dbf0c1e41c",
                 "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                 "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                 "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                 "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                "Log.out:md5,23f80ce482ce4779ff88c129e4b6a6e5",
                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -283,13 +267,11 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "genomeParameters.txt:md5,f9e87dbcaa7bc8ea9df576ba3ed3bbd0",
                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                "trans.blast.align_coords.align_coords.dbm:md5,a15f136b73f31fd474450e70e7e85e7b",
                 "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
             ]
         ],
@@ -297,6 +279,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-19T13:09:57.878292804"
+        "timestamp": "2025-03-19T14:46:22.709176112"
     }
 }
\ No newline at end of file

From bd22559f8ea355148f41a5fc83ca33c677ab533f Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 20 Mar 2025 10:09:07 +0000
Subject: [PATCH 352/887] add an error message for conda/mamba runs

---
 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 378b89f1b..23184d336 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -163,6 +163,11 @@ def validateInputParameters() {
             error("No fusion annotation library provided. `STARFUSION_BUILD` is unable to run.")
     }
 
+    def profiles = workflow.profile
+    if ((profiles.contains("conda") || profiles.contains("mamba")) && (params.ctatsplicing || params.all)) {
+        error("Conda or Mamba runs are not supported when using --ctatsplicing or --all")
+    }
+
 }
 
 //

From d96374a9a07bca61694c3dc6372a079e149a3a9e Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 20 Mar 2025 14:06:19 +0100
Subject: [PATCH 353/887] fix a small issue with the qc workflow input

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 9e2c1ff8f..de347fcfc 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -197,7 +197,7 @@ workflow RNAFUSION {
 
         //QC
         QC_WORKFLOW (
-            ch_aligned_reads,
+            ch_aligned_reads.map { meta, bam, _bai -> [meta, bam] },
             BUILD_REFERENCES.out.ch_refflat,
             BUILD_REFERENCES.out.ch_fasta,
             BUILD_REFERENCES.out.ch_fai,

From 70d1b14c3865d2ddad1bde66d219e3f15470293b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 20 Mar 2025 14:16:11 +0100
Subject: [PATCH 354/887] update docs

---
 docs/output.md | 113 +++++++------------------------------------------
 1 file changed, 15 insertions(+), 98 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 3698f7a69..c9f8170c0 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -72,8 +72,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 {outdir}
 ├── arriba
 ├── arriba_visualisation
-├── cram_arriba
-├── cram_starfusion
 ├── fastp
 ├── fastqc
 ├── fusioncatcher
@@ -85,8 +83,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 ├── picard
 ├── pipeline_info
 ├── samtools_sort_for_arriba
-├── star_for_arriba
-├── star_for_starfusion
+├── star
 ├── starfusion
 └── work
 .nextflow.log
@@ -348,112 +345,32 @@ Picard CollectRnaMetrics and picard MarkDuplicates share the same output directo
 
 </details>
 
-### Samtools
-
-#### Samtools sort
-
-Samtools sort is used to sort BAM files from STAR_FOR_STARFUSION (for arriba visualisation)
-
-<details markdown="1">
-<summary>Output files</summary>
-
-- `samtools_sort_for_<arriba>`
-  - `<sample>(_chimeric)_sorted.bam` - sorted BAM file
-
-</details>
-
-#### Samtools index
-
-Samtools index is used to index BAM files from STAR_FOR_ARRIBA (for arriba visualisation) and STAR_FOR_STARFUSION (for QC)
-
-<details markdown="1">
-<summary>Output files</summary>
-
-- `samtools_for_<arriba/qc>`
-  - `<sample>.(Aligned.sortedByCoord).out.bam.bai` -
-
-</details>
-
 ### STAR
 
-STAR is used to align to genome reference
-
-STAR is run for 3 tools:
-
-For `arriba` with the parameters:
-
-```bash
---readFilesCommand zcat \
---outSAMtype BAM Unsorted \
---outSAMunmapped Within \
---outBAMcompression 0 \
---outFilterMultimapNmax 50 \
---peOverlapNbasesMin 10 \
---alignSplicedMateMapLminOverLmate 0.5 \
---alignSJstitchMismatchNmax 5 -1 5 5 \
---chimSegmentMin 10 \
---chimOutType WithinBAM HardClip \
---chimJunctionOverhangMin 10 \
---chimScoreDropMax 30 \
---chimScoreJunctionNonGTAG 0 \
---chimScoreSeparation 1 \
---chimSegmentReadGapMax 3 \
---chimMultimapNmax 50
-```
-
-For `STAR-fusion` with the parameters:
-
-```bash
---twopassMode Basic \
---outReadsUnmapped None \
---readFilesCommand zcat \
---outSAMstrandField intronMotif \
---outSAMunmapped Within \
---chimSegmentMin 12 \
---chimJunctionOverhangMin 8 \
---chimOutJunctionFormat 1 \
---alignSJDBoverhangMin 10 \
---alignMatesGapMax 100000 \
---alignIntronMax 100000 \
---alignSJstitchMismatchNmax 5 -1 5 5 \
---chimMultimapScoreRange 3 \
---chimScoreJunctionNonGTAG -4 \
---chimMultimapNmax 20 \
---chimNonchimScoreDropMin 10 \
---peOverlapNbasesMin 12 \
---peOverlapMMp 0.1 \
---alignInsertionFlush Right \
---alignSplicedMateMapLminOverLmate 0 \
---alignSplicedMateMapLmin 30 \
---chimOutType Junctions \
---quantMode GeneCounts
-```
+STAR is used to align FASTQ files to the genome reference.
 
-> STAR_FOR_STARFUSION uses `${params.ensembl}/Homo_sapiens.GRCh38.${params.ensembl_version}.chr.gtf` whereas STAR_FOR_ARRIBA uses `${params.ensembl_ref}/Homo_sapiens.GRCh38.${params.ensembl_version}.gtf`
+Additionally, CRAM files can also be created when passing the `--cram` option. The CRAM conversion is done with a combination of `samtools view` and `samtools index`.
 
 <details markdown="1">
 <summary>Output files</summary>
 
 **Common**
 
-- `star_for_<tool>`
-- `<sample>.Log.final.out`
-- `<sample>.Log.progress.out`
-- `<sample>.SJ.out.tab`
-
-**For arriba:**
-
-- `<sample>.Aligned.out.bam`
-
-  **For starfusion:**
-
-- `<sample>.Aligned.sortedByCoord.out.bam`
-- `<sample>.Chimeric.out.junction`
-- `<sample>.ReadsPerGene.out.tab`
+- `star`
+  - `<sample>.Aligned.sortedByCoord.out.bam`
+  - `<sample>.Aligned.sortedByCoord.out.bam.bai`
+  - `<sample>.Aligned.sortedByCoord.out.cram` - when `--cram` is used
+  - `<sample>.Aligned.sortedByCoord.out.cram.crai` - when `--cram` is used
+  - `<sample>.Chimeric.out.junction`
+  - `<sample>.Log.final.out`
+  - `<sample>.Log.out`
+  - `<sample>.Log.progress.out`
+  - `<sample>.ReadsPerGene.out.tab`
+  - `<sample>.SJ.out.tab`
 
 </details>
 
-The STAR index is generated with `--sjdbOverhang ${params.read_length - 1}`, params.read_length default is 100.
+The STAR index is generated with `--sjdbOverhang ${params.read_length - 1}`, `params.read_length` default is 100.
 
 ### STAR-fusion
 

From 0ec782cd8a92ffd67b6b2c56433105e148d81291 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=C3=96mer=20An?= <bounlu@gmail.com>
Date: Tue, 25 Mar 2025 12:28:40 +0800
Subject: [PATCH 355/887] Fixed HGNC download link

Fixes https://github.com/nf-core/rnafusion/issues/638
---
 modules/local/hgnc/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 1b3808f6d..8783bb179 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -18,7 +18,7 @@ process HGNC_DOWNLOAD {
 
     script:
     """
-    wget https://ftp.ebi.ac.uk/pub/databases/genenames/hgnc/tsv/hgnc_complete_set.txt
+    wget https://storage.googleapis.com/public-download-files/hgnc/tsv/tsv/hgnc_complete_set.txt
     date +%Y-%m-%d/%H:%M  > HGNC-DB-timestamp.txt
 
     cat <<-END_VERSIONS > versions.yml

From 9c14acdcebdb89c1f3bdf806a615684b2bb6fbd4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 25 Mar 2025 10:23:34 +0100
Subject: [PATCH 356/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d6b2f6496..ab925dbd0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -50,6 +50,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix error message when parameter outdir is missing [#611](https://github.com/nf-core/rnafusion/pull/611)
 - Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
 - Update htslib and samtools version in `star/align` to 1.21 [#634](https://github.com/nf-core/rnafusion/pull/634)
+- Updated Zenodo DOI in badge [#639](https://github.com/nf-core/rnafusion/pull/639)
 
 ### Fixed
 

From 321135e034ff48aa82cfba611f17882c6acb760f Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 25 Mar 2025 09:56:25 +0000
Subject: [PATCH 357/887] bump parameters schema

---
 nextflow.config      |  1 -
 nextflow_schema.json | 95 ++++++++++++++++++++++++++++++++++++--------
 2 files changed, 78 insertions(+), 18 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index c14bba0ca..aac49eecb 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -78,7 +78,6 @@ params {
     gtf                           = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf"
     refflat                       = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf.refflat"
     rrna_intervals                = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.interval_list"
-    gencode_ref                   = "${params.genomes_base}/gencode"
     no_cosmic                     = false
     arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz"
     arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index aaf8a64ab..8ef4a0948 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -42,9 +42,9 @@
                     "type": "string",
                     "format": "file-path",
                     "schema": "assets/schema_input.json",
-                    "mimetype": "text/csv",
-                    "pattern": "^\\S+\\.csv$",
-                    "description": "Path to comma-separated file containing information about the samples in the experiment.",
+                    "exists": true,
+                    "pattern": "^\\S+\\.(csv|yaml|yml|json)$",
+                    "description": "Path to samplesheet file containing information about the samples in the experiment.",
                     "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/rnafusion/usage#samplesheet-input).",
                     "fa_icon": "fas fa-file-csv"
                 },
@@ -94,8 +94,9 @@
                 "read_length": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
-                    "description": "Read length",
-                    "default": 100
+                    "description": "The length of the reads provided to the pipeline. This is used for the '--sjdbOverhang' option of STAR as read_length - 1. Providing 1 to this option will disable overhang handling.",
+                    "default": 100,
+                    "minimum": 1
                 },
                 "all": {
                     "type": "boolean",
@@ -109,26 +110,41 @@
                 },
                 "arriba_ref_blacklist": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.tsv\\.gz$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
                 },
                 "arriba_ref_cytobands": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference cytobands"
                 },
                 "arriba_ref_known_fusions": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.tsv\\.gz$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference known fusions"
                 },
                 "arriba_ref_protein_domains": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.gff3$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference protein domain"
                 },
                 "arriba_fusions": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba output"
                 },
@@ -142,11 +158,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Run CTAT-splicing to detect abberant cancer splicing introns. Needs --arriba and/or --starfusion to run."
                 },
-                "gencode_ref": {
-                    "type": "string",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to gencode references"
-                },
                 "fusioncatcher": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -154,28 +165,38 @@
                 },
                 "fusioncatcher_fusions": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.txt$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher output"
                 },
                 "fusioncatcher_limitSjdbInsertNsj": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
-                    "description": "Use limitSjdbInsertNsj with int for fusioncatcher"
+                    "description": "Use limitSjdbInsertNsj with int for fusioncatcher",
+                    "default": 2000000,
+                    "minimum": 1
                 },
                 "fusioncatcher_ref": {
                     "type": "string",
+                    "format": "directory-path",
+                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
                 "fusioncatcher_ref_stub_check": {
                     "type": "string",
+                    "hidden": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to file in fusioncatcher references"
                 },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
-                    "description": "Use limitSjdbInsertNsj with int for fusioninspector STAR process"
+                    "description": "Use limitSjdbInsertNsj with int for fusioninspector STAR process",
+                    "default": 1000000,
+                    "minimum": 1
                 },
                 "fusioninspector_only": {
                     "type": "boolean",
@@ -184,6 +205,9 @@
                 },
                 "fusioninspector_fusions": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to a fusion list file built with format GENE1--GENE2"
                 },
@@ -194,21 +218,30 @@
                 },
                 "fusionreport_ref": {
                     "type": "string",
+                    "format": "directory-path",
+                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusionreport references"
                 },
                 "fusionreport_ref_stub_check": {
                     "type": "string",
+                    "hidden": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to file in fusionreport references"
                 },
                 "hgnc_ref": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.txt$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to HGNC database file"
                 },
                 "hgnc_date": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.txt$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to HGNC timestamp file for database retrieval"
                 },
@@ -219,11 +252,14 @@
                 },
                 "salmon_index": {
                     "type": "string",
+                    "format": "directory-path",
+                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to salmon index"
                 },
                 "salmon_index_stub_check": {
                     "type": "string",
+                    "hidden": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to file in salmon index"
                 },
@@ -234,22 +270,29 @@
                 },
                 "starfusion_fusions": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starfusion output"
                 },
                 "starfusion_ref": {
                     "type": "string",
+                    "format": "directory-path",
+                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starfusion references"
                 },
                 "starfusion_ref_stub_check": {
                     "type": "string",
+                    "hidden": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to file in starfusion references"
                 },
                 "ctatsplicing_cancer_introns": {
                     "type": "string",
                     "format": "file-path",
+                    "pattern": "\\S+\\.tsv\\.gz$",
                     "exists": true,
                     "description": "Path to the cancer introns CSV file to create the CTAT-SPLICING reference with",
                     "default": "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
@@ -261,11 +304,14 @@
                 },
                 "starindex_ref": {
                     "type": "string",
+                    "format": "directory-path",
+                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starindex references"
                 },
                 "starindex_ref_stub_check": {
                     "type": "string",
+                    "hidden": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to file in starindex references"
                 },
@@ -276,11 +322,16 @@
                 },
                 "tools_cutoff": {
                     "type": "integer",
+                    "minimum": 1,
+                    "default": 1,
                     "fa_icon": "far fa-file-code",
                     "description": "Discard fusions identified by less than INT tools"
                 },
                 "whitelist": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusions to add to the input of fusioninspector"
                 }
@@ -299,11 +350,15 @@
                 },
                 "trim_tail": {
                     "type": "integer",
-                    "description": "Preform tail trimming of reads, default: null",
+                    "description": "The amount of bases to trim at the tail of each read, none will be trimmed by default",
+                    "minimum": 1,
                     "fa_icon": "fas fa-cut"
                 },
                 "adapter_fasta": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.f(n|ast)?a$",
                     "description": "Path to adapter fasta file: default: []",
                     "fa_icon": "fas fa-cut"
                 }
@@ -336,6 +391,7 @@
                 "fasta": {
                     "type": "string",
                     "format": "file-path",
+                    "exists": true,
                     "mimetype": "text/plain",
                     "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
                     "description": "Path to FASTA genome file.",
@@ -345,6 +401,7 @@
                     "type": "string",
                     "format": "file-path",
                     "mimetype": "text/plain",
+                    "exists": true,
                     "pattern": "^\\S+\\.fn?ai(\\.gz)?$",
                     "description": "Path to FASTA genome index file.",
                     "fa_icon": "far fa-file-code"
@@ -357,7 +414,7 @@
                 "gtf": {
                     "type": "string",
                     "format": "file-path",
-                    "mimetype": "text/plain",
+                    "exists": true,
                     "pattern": "^\\S+\\.gtf?(\\.gz)?$",
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
@@ -365,7 +422,7 @@
                 "refflat": {
                     "type": "string",
                     "format": "file-path",
-                    "mimetype": "text/plain",
+                    "exists": true,
                     "pattern": "^\\S+\\.refflat?$",
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
@@ -373,7 +430,7 @@
                 "rrna_intervals": {
                     "type": "string",
                     "format": "file-path",
-                    "mimetype": "text/plain",
+                    "exists": true,
                     "pattern": "^\\S+\\.interval_list?$",
                     "description": "Path to ribosomal interval list.",
                     "fa_icon": "far fa-file-code"
@@ -385,13 +442,17 @@
                 },
                 "fusion_annot_lib": {
                     "type": "string",
+                    "format": "file-path",
+                    "exists": true,
+                    "pattern": "\\S+\\.dat\\.gz$",
                     "description": "Path to Fusion Annotation Library to be used in STARFUSION_BUILD.",
                     "fa_icon": "far fa-file-code"
                 },
                 "species": {
                     "type": "string",
                     "description": "Which species dfam should automatically download, default: human.",
-                    "fa_icon": "far fa-file-code"
+                    "fa_icon": "far fa-file-code",
+                    "default": "human"
                 }
             }
         },

From 23c5da43cebc3b857eb0ef6d03e32cabfc303099 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 25 Mar 2025 09:58:43 +0000
Subject: [PATCH 358/887] bump samplesheet schema

---
 assets/schema_input.json | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index a55008106..f38d29c48 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -10,7 +10,7 @@
             "sample": {
                 "type": "string",
                 "pattern": "^\\S+$",
-                "errorMessage": "Sample name must be provided and cannot contain spaces",
+                "errorMessage": "Sample name must be provided, has to be a string and cannot contain spaces",
                 "meta": ["id"]
             },
             "fastq_1": {
@@ -18,19 +18,19 @@
                 "format": "file-path",
                 "exists": true,
                 "pattern": "^\\S+f(ast)?q\\.gz$",
-                "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
+                "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces, has to exist and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
                 "pattern": "^\\S+f(ast)?q\\.gz$",
-                "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
+                "errorMessage": "FastQ file for reads 2 cannot contain spaces, has to exist and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "strandedness": {
                 "type": "string",
                 "enum": ["forward", "reverse", "unstranded", "unknown"],
-                "errorMessage": "Strandedness has to be forward, reverse, unstranded or unknown"
+                "errorMessage": "Strandedness has to be 'forward', 'reverse', 'unstranded' or 'unknown'"
             }
         },
         "required": ["sample", "fastq_1", "fastq_2", "strandedness"]

From 1d92db882f9204bb62851ccc4dd83f7ba087f7cb Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 25 Mar 2025 10:25:52 +0000
Subject: [PATCH 359/887] remove some exists checks

---
 nextflow_schema.json | 16 ----------------
 1 file changed, 16 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 8ef4a0948..7f8ed19ae 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -111,7 +111,6 @@
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.tsv\\.gz$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
@@ -119,7 +118,6 @@
                 "arriba_ref_cytobands": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference cytobands"
@@ -127,7 +125,6 @@
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.tsv\\.gz$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference known fusions"
@@ -135,7 +132,6 @@
                 "arriba_ref_protein_domains": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.gff3$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference protein domain"
@@ -181,7 +177,6 @@
                 "fusioncatcher_ref": {
                     "type": "string",
                     "format": "directory-path",
-                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
@@ -219,7 +214,6 @@
                 "fusionreport_ref": {
                     "type": "string",
                     "format": "directory-path",
-                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusionreport references"
                 },
@@ -232,7 +226,6 @@
                 "hgnc_ref": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.txt$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to HGNC database file"
@@ -240,7 +233,6 @@
                 "hgnc_date": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.txt$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to HGNC timestamp file for database retrieval"
@@ -253,7 +245,6 @@
                 "salmon_index": {
                     "type": "string",
                     "format": "directory-path",
-                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to salmon index"
                 },
@@ -279,7 +270,6 @@
                 "starfusion_ref": {
                     "type": "string",
                     "format": "directory-path",
-                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starfusion references"
                 },
@@ -305,7 +295,6 @@
                 "starindex_ref": {
                     "type": "string",
                     "format": "directory-path",
-                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starindex references"
                 },
@@ -391,7 +380,6 @@
                 "fasta": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "mimetype": "text/plain",
                     "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$",
                     "description": "Path to FASTA genome file.",
@@ -401,7 +389,6 @@
                     "type": "string",
                     "format": "file-path",
                     "mimetype": "text/plain",
-                    "exists": true,
                     "pattern": "^\\S+\\.fn?ai(\\.gz)?$",
                     "description": "Path to FASTA genome index file.",
                     "fa_icon": "far fa-file-code"
@@ -414,7 +401,6 @@
                 "gtf": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "^\\S+\\.gtf?(\\.gz)?$",
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
@@ -422,7 +408,6 @@
                 "refflat": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "^\\S+\\.refflat?$",
                     "description": "Path to GTF genome file.",
                     "fa_icon": "far fa-file-code"
@@ -430,7 +415,6 @@
                 "rrna_intervals": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "^\\S+\\.interval_list?$",
                     "description": "Path to ribosomal interval list.",
                     "fa_icon": "far fa-file-code"

From f8903fd628bb2bba9036e5b7d9cb82eef41baad2 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 25 Mar 2025 10:27:19 +0000
Subject: [PATCH 360/887] bump changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d6b2f6496..765511db7 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -62,6 +62,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed bug in `FUSIONCATCHER_DOWNLOAD` that caused an error when running with singularity profile [#573](https://github.com/nf-core/rnafusion/issues/573)
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
 - Fixed the codebase to be compatible with the Nextflow language server [#634](https://github.com/nf-core/rnafusion/pull/634)
+- Updated the input validation to be more strict. This will prevent more errors down the line in the pipeline [#640](https://github.com/nf-core/rnafusion/pull/640)
 
 ### Removed
 

From 4692a4aaba1b6bdac6592aea0cb8e7354aafd377 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 25 Mar 2025 12:12:22 +0100
Subject: [PATCH 361/887] fix spelling

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index ab925dbd0..5821e8550 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -23,7 +23,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Add nf-test to local subworkflow: `ARRIBA_WORKFLOW` [#578](https://github.com/nf-core/rnafusion/pull/578)
 - Add nf-test to local module: `STARFUSION_BUILD`. [#585](https://github.com/nf-core/rnafusion/pull/585)
 - Add nf-test to local module: `STARFUSION_DETECT`. [#586](https://github.com/nf-core/rnafusion/pull/586)
-- Added a new module `CTATSPLICING_STARTOCANCERINTRONS` and a new parameter `--ctatsplicing`. This options creates reports on cancer splicing abberations and requires one or both of `--arriba` and `--starfusion` to be given. [#587](https://github.com/nf-core/rnafusion/pull/587)
+- Added a new module `CTATSPLICING_STARTOCANCERINTRONS` and a new parameter `--ctatsplicing`. This options creates reports on cancer splicing aberrations and requires one or both of `--arriba` and `--starfusion` to be given. [#587](https://github.com/nf-core/rnafusion/pull/587)
 - Add parameter `--references_only` when no data should be analysed, but only the references should be built [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Add nf-test to local subworkflow: `FUSIONCATCHER_WORKFLOW` [#591](https://github.com/nf-core/rnafusion/pull/591)
 - Add nf-test to local subworkflow: `STARFUSION_WORKFLOW`. [#597](https://github.com/nf-core/rnafusion/pull/597)

From 8a7b54d126a48a0024a174207166ab3bd84881a1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 25 Mar 2025 12:15:56 +0100
Subject: [PATCH 362/887] run_fusioncatcher back to fusioncatcher

---
 subworkflows/local/fusioncatcher_workflow/main.nf | 6 +++---
 workflows/rnafusion.nf                            | 2 +-
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index b03908e35..35e74030c 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -3,13 +3,13 @@ include { FUSIONCATCHER_DETECT } from '../../../modules/local/fusioncatcher/dete
 // TODO: Remove fusioncatcher_fusions as parameter.
 // TODO: remove dummy file. Work with Channel.empty()
 // TODO: if the files were already produced and the user want to skip the module because of this, they should be taken them from the sample sheet
-// TODO: harmonize `run_fusioncatcher` and `fusioncatcher_only` parameters at main workflow level to activate/skip this one.
+// TODO: harmonize `fusioncatcher` and `fusioncatcher_only` parameters at main workflow level to activate/skip this one.
 
 workflow FUSIONCATCHER_WORKFLOW {
     take:
         reads                   // channel [ meta, [ fastqs ] ]
         fusioncatcher_ref       // channel [ meta, path       ]
-        run_fusioncatcher       // boolean
+        fusioncatcher          // boolean
         all                     // boolean
         fusioninspector_only    // boolean
         fusioncatcher_fusions   // path, string
@@ -18,7 +18,7 @@ workflow FUSIONCATCHER_WORKFLOW {
         ch_versions   = Channel.empty()
         ch_dummy_file = file("$baseDir/assets/dummy_file_fusioncatcher.txt", checkIfExists: true)
 
-        if (( run_fusioncatcher || all) && !fusioninspector_only ) {
+        if (( fusioncatcher || all) && !fusioninspector_only ) {
             if (fusioncatcher_fusions){
 
                 ch_fusioncatcher_fusions = reads.combine(Channel.value(file(fusioncatcher_fusions, checkIfExists:true)))
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ddd006ba3..1cfa6cba3 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -122,7 +122,7 @@ workflow RNAFUSION {
     FUSIONCATCHER_WORKFLOW (
         ch_reads,
         BUILD_REFERENCES.out.ch_fusioncatcher_ref,       // channel [ meta, path       ]
-        params.run_fusioncatcher,
+        params.fusioncatcher,
         params.all,
         params.fusioninspector_only,
         params.fusioncatcher_fusions

From c416e1b85e741e2506f911ad1127b2ab47ee22cc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 25 Mar 2025 12:17:36 +0100
Subject: [PATCH 363/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d6b2f6496..d2c13b9bb 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -50,6 +50,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix error message when parameter outdir is missing [#611](https://github.com/nf-core/rnafusion/pull/611)
 - Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
 - Update htslib and samtools version in `star/align` to 1.21 [#634](https://github.com/nf-core/rnafusion/pull/634)
+- `--run_fusioncatcher` back to `fusioncatcher` [#641](https://github.com/nf-core/rnafusion/pull/641)
 
 ### Fixed
 

From 73946e6fd23ba103d0d5b33ea805d67fb1faf117 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?=C3=96mer=20An?= <bounlu@gmail.com>
Date: Wed, 26 Mar 2025 11:51:11 +0800
Subject: [PATCH 364/887] Fixed typo

---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index fe370b363..8c2da3989 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -47,7 +47,7 @@ nextflow run nf-core/rnafusion \
   --outdir <OUTPUT/PATH>
 ```
 
-### Downloading the cosmic database with SANGER or QUIAGEN
+### Downloading the cosmic database with SANGER or QIAGEN
 
 #### For academic users
 

From 17cfa8f817d1589bb7a0b5a7d8f3ceccf6dd5544 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 27 Mar 2025 10:24:02 +0000
Subject: [PATCH 365/887] fix test_stub

---
 tests/test_stub.nf.test.snap | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index a2bb8a80d..4e1cf6bb2 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,7 +1,7 @@
 {
     "stub test no fastp trim": {
         "content": [
-            32,
+            30,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -330,11 +330,11 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-17T17:40:40.562156411"
+        "timestamp": "2025-03-27T10:23:24.571345349"
     },
     "stub test with fastp trim": {
         "content": [
-            34,
+            32,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -678,6 +678,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-17T17:38:45.56378377"
+        "timestamp": "2025-03-27T10:22:34.410397222"
     }
 }
\ No newline at end of file

From e33d8fd153745a8a41140cd42539a9881cad377e Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 15:48:05 +0200
Subject: [PATCH 366/887] simplify build_references

---
 main.nf                                | 58 +++++++++----------------
 subworkflows/local/build_references.nf | 60 +++++++++++++-------------
 workflows/rnafusion.nf                 |  3 +-
 3 files changed, 54 insertions(+), 67 deletions(-)

diff --git a/main.nf b/main.nf
index 96e564d3d..710845900 100644
--- a/main.nf
+++ b/main.nf
@@ -20,41 +20,6 @@ include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_rnaf
 include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { RNAFUSION               } from './workflows/rnafusion'
 
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    GENOME PARAMETER VALUES
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-
-
-
-
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    NAMED WORKFLOWS FOR PIPELINE
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-*/
-
-//
-// WORKFLOW: Run main analysis pipeline depending on type of input
-//
-workflow NFCORE_RNAFUSION {
-    take:
-    samplesheet
-
-    main:
-
-    //
-    // WORKFLOW: Run pipeline
-    //
-
-    RNAFUSION(samplesheet)
-
-    emit:
-    multiqc_report = RNAFUSION.out.multiqc_report
-}
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     RUN MAIN WORKFLOW
@@ -74,10 +39,29 @@ workflow {
         params.outdir,
     )
 
+    // A list of all tools available in the pipeline. Add tools here when a new tool has been added
+    def all_tools = [
+        "arriba",
+        "ctatsplicing",
+        "fusioncatcher",
+        "starindex",
+        "starfusion",
+        "stringtie",
+        "fusion_report",
+        "fusioninspector_only"
+    ]
+
+    def tools = params.all ? all_tools : all_tools.findAll { tool ->
+        params.get(tool)
+    }
+
     //
     // WORKFLOW: Run main workflow
     //
-    NFCORE_RNAFUSION (PIPELINE_INITIALISATION.out.samplesheet)
+    RNAFUSION(
+        PIPELINE_INITIALISATION.out.samplesheet,
+        tools
+    )
 
     //
     // SUBWORKFLOW: Run completion tasks
@@ -89,7 +73,7 @@ workflow {
         params.outdir,
         params.monochrome_logs,
         params.hook_url,
-        NFCORE_RNAFUSION.out.multiqc_report
+        RNAFUSION.out.multiqc_report
     )
 }
 
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 6ad2c1906..0e2963bc5 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -34,11 +34,13 @@ include { GFFREAD }                         from '../../modules/nf-core/gffread/
 
 workflow BUILD_REFERENCES {
 
+    take:
+    tools // list of all the tools to create references from
+
     main:
     ch_versions = Channel.empty()
 
-    if (!file(params.fasta).exists() || file(params.fasta).isEmpty() ||
-            !file(params.gtf).exists() || file(params.gtf).isEmpty()){
+    if (!exists_not_empty(params.fasta) || !exists_not_empty(params.gtf)){
         GENCODE_DOWNLOAD(params.genome_gencode_version, params.genome)
         ch_versions = ch_versions.mix(GENCODE_DOWNLOAD.out.versions)
         ch_fasta = GENCODE_DOWNLOAD.out.fasta.map { that -> [[id:that.Name], that] }
@@ -48,7 +50,7 @@ workflow BUILD_REFERENCES {
         ch_gtf = Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }
     }
 
-    if (!file(params.fai).exists() || file(params.fai).isEmpty()){
+    if (!exists_not_empty(params.fai)){
         SAMTOOLS_FAIDX(ch_fasta, [[],[]])
         ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions)
         ch_fai = SAMTOOLS_FAIDX.out.fai
@@ -56,8 +58,7 @@ workflow BUILD_REFERENCES {
         ch_fai = Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
     }
 
-    if ((!file(params.hgnc_ref).exists() || file(params.hgnc_ref).isEmpty() ||
-            !file(params.hgnc_date).exists() || file(params.hgnc_date).isEmpty()) && !params.skip_vcf){
+    if ((!exists_not_empty(params.hgnc_ref) || !exists_not_empty(params.hgnc_date)) && !params.skip_vcf){
         HGNC_DOWNLOAD( )
         ch_versions = ch_versions.mix(HGNC_DOWNLOAD.out.versions)
         ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref
@@ -67,7 +68,7 @@ workflow BUILD_REFERENCES {
         ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
     }
 
-    if (!file(params.rrna_intervals).exists() || file(params.rrna_intervals).isEmpty()){
+    if (!exists_not_empty(params.rrna_intervals)){
         GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
         ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
         GET_RRNA_TRANSCRIPTS(ch_gtf)
@@ -79,7 +80,7 @@ workflow BUILD_REFERENCES {
         ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
     }
 
-    if (!file(params.refflat).exists() || file(params.refflat).isEmpty()){
+    if (!exists_not_empty(params.refflat)){
         GTF_TO_REFFLAT(ch_gtf)
         ch_versions = ch_versions.mix(GTF_TO_REFFLAT.out.versions)
         ch_refflat = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
@@ -87,8 +88,7 @@ workflow BUILD_REFERENCES {
         ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
     }
 
-    if (!file(params.salmon_index).exists() || file(params.salmon_index).isEmpty() ||
-        !file(params.salmon_index_stub_check).exists() || file(params.salmon_index_stub_check).isEmpty()){ // add condition for qc
+    if (!exists_not_empty(params.salmon_index) || !exists_not_empty(params.salmon_index_stub_check)){ // add condition for qc
         GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
         ch_versions = ch_versions.mix(GFFREAD.out.versions)
         SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
@@ -98,9 +98,8 @@ workflow BUILD_REFERENCES {
         ch_salmon_index = Channel.fromPath({params.salmon_index})
     }
 
-    if ((params.starindex || params.all || params.starfusion || params.arriba) &&
-            (!file(params.starindex_ref).exists() || file(params.starindex_ref).isEmpty() ||
-            !file(params.starindex_ref_stub_check).exists() || file(params.starindex_ref_stub_check).isEmpty() )) {
+    def star_index_tools = tools.intersect(["starindex", "starfusion", "arriba", "ctatsplicing"])
+    if (star_index_tools.size() > 0 && (!exists_not_empty(params.starindex_ref) || !exists_not_empty(params.starindex_ref_stub_check))) {
         STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
         ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions)
         ch_starindex_ref = STAR_GENOMEGENERATE.out.index
@@ -108,10 +107,12 @@ workflow BUILD_REFERENCES {
         ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
     }
 
-    if ((params.arriba || params.all) &&
-            (!file(params.arriba_ref_blacklist).exists() || file(params.arriba_ref_blacklist).isEmpty() ||
-            !file(params.arriba_ref_known_fusions).exists() || file(params.arriba_ref_known_fusions).isEmpty() ||
-            !file(params.arriba_ref_protein_domains).exists() || file(params.arriba_ref_protein_domains).isEmpty())) {
+    if (tools.contains("arriba") && (
+            !exists_not_empty(params.arriba_ref_blacklist) ||
+            !exists_not_empty(params.arriba_ref_known_fusions) ||
+            !exists_not_empty(params.arriba_ref_protein_domains)
+        )
+    ) {
         ARRIBA_DOWNLOAD(params.genome)
         ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)
         ch_arriba_ref_blacklist = ARRIBA_DOWNLOAD.out.blacklist
@@ -126,9 +127,7 @@ workflow BUILD_REFERENCES {
     }
 
 
-    if ((params.fusioncatcher || params.all) &&
-            (!file(params.fusioncatcher_ref).exists() || file(params.fusioncatcher_ref).isEmpty() ||
-            !file(params.fusioncatcher_ref_stub_check).exists() || file(params.fusioncatcher_ref_stub_check).isEmpty() )) {
+    if (tools.contains("fusioncatcher") && (!exists_not_empty(params.fusioncatcher_ref) || !exists_not_empty(params.fusioncatcher_ref_stub_check))) {
             FUSIONCATCHER_BUILD(params.genome_gencode_version)
             ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
             ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
@@ -137,13 +136,11 @@ workflow BUILD_REFERENCES {
         ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref)
     }
 
-
-    if ((params.starfusion || params.all) &&
-            (!file(params.starfusion_ref).exists() || file(params.starfusion_ref).isEmpty() ||
-            !file(params.starfusion_ref_stub_check).exists() || file(params.starfusion_ref_stub_check).isEmpty() )) {
+    def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing"])
+    if (starfusion_tools.size() > 0 && (!exists_not_empty(params.starfusion_ref) || !exists_not_empty(params.starfusion_ref_stub_check))) {
             STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
-            if (params.ctatsplicing || params.all) {
+            if (tools.contains("ctatsplicing")) {
                 CTATSPLICING_PREPGENOMELIB(
                     STARFUSION_BUILD.out.reference,
                     params.ctatsplicing_cancer_introns
@@ -159,10 +156,10 @@ workflow BUILD_REFERENCES {
     }
 
 
-    if ((params.fusionreport || params.all) &&
-            (!file(params.fusionreport_ref).exists() || file(params.fusionreport_ref).isEmpty() ||
-            !file(params.fusionreport_ref_stub_check).exists() || file(params.fusionreport_ref_stub_check).isEmpty())) {
-        if (!params.no_cosmic && (!params.cosmic_username || !params.cosmic_passwd)) { exit 1, 'COSMIC username and/or password missing' }
+    if (tools.contains("fusionreport") && (!exists_not_empty(params.fusionreport_ref) || !exists_not_empty(params.fusionreport_ref_stub_check))) {
+        if (!params.no_cosmic && (!params.cosmic_username || !params.cosmic_passwd)) {
+            error('COSMIC username and/or password missing, this is needed to download the fusionreport reference')
+        }
         FUSIONREPORT_DOWNLOAD()
         ch_versions = ch_versions.mix(FUSIONREPORT_DOWNLOAD.out.versions)
         ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
@@ -192,6 +189,11 @@ workflow BUILD_REFERENCES {
 
 /*
 ========================================================================================
-    THE END
+    FUNCTIONS
 ========================================================================================
 */
+
+def exists_not_empty(path) {
+    def path_to_check = file(path)
+    return path_to_check.exists() && !path_to_check.isEmpty()
+}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 1cfa6cba3..48bb147f0 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -35,6 +35,7 @@ workflow RNAFUSION {
 
     take:
     ch_samplesheet // channel: samplesheet read in from --input
+    tools          // list: a list of tools to run
 
     main:
 
@@ -45,7 +46,7 @@ workflow RNAFUSION {
     // Create references if necessary
     //
 
-    BUILD_REFERENCES()
+    BUILD_REFERENCES(tools)
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
     if (!params.references_only) { // TODO: Remove this temporary parameter when we have a full-working GitHub nf-test

From a062c9bb83c4371cf4e1de154897c5f912e01776 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 16:12:05 +0200
Subject: [PATCH 367/887] arriba workflow

---
 main.nf                                    |   2 +-
 nextflow.config                            |   1 +
 nextflow_schema.json                       |   5 +
 subworkflows/local/arriba_workflow/main.nf | 106 +++++++++------------
 workflows/rnafusion.nf                     |  40 ++++----
 5 files changed, 72 insertions(+), 82 deletions(-)

diff --git a/main.nf b/main.nf
index 710845900..c2f826aff 100644
--- a/main.nf
+++ b/main.nf
@@ -48,7 +48,7 @@ workflow {
         "starfusion",
         "stringtie",
         "fusion_report",
-        "fusioninspector_only"
+        "fusioninspector"
     ]
 
     def tools = params.all ? all_tools : all_tools.findAll { tool ->
diff --git a/nextflow.config b/nextflow.config
index aac49eecb..e8067f759 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -62,6 +62,7 @@ params {
     stringtie                  = false
     fusionreport               = false
     fusioninspector_only       = false
+    fusioninspector            = false
 
     // Skip steps
     skip_qc                    = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 7f8ed19ae..297f64055 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -198,6 +198,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Skip fusion-report. --fusioninspector_fusions PATH needed to provide a fusion list as input"
                 },
+                "fusioninspector": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Run fusioninspector"
+                },
                 "fusioninspector_fusions": {
                     "type": "string",
                     "format": "file-path",
diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 84ba3604f..0c46608f7 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -17,11 +17,8 @@ workflow ARRIBA_WORKFLOW {
         ch_arriba_ref_known_fusions     // channel [ meta, path_known_fusions ]
         ch_arriba_ref_protein_domains   // channel [ meta, path_proteins      ]
         ch_starfusion_ref               // channel [ meta, path_starfusion_ref ]
-        arriba                          // boolean
-        all                             // boolean
-        fusioninspector_only            // boolean
+        tools                           // list    A list of all tools to run
         star_ignore_sjdbgtf             // boolean
-        ctatsplicing                    // boolean
         seq_center                      // string
         arriba_fusions                  // path
         cram                            // array
@@ -32,74 +29,63 @@ workflow ARRIBA_WORKFLOW {
         def ch_cram_index = Channel.empty()
         def ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
-        if (( arriba || all ) && !fusioninspector_only) {
-
-            STAR_FOR_ARRIBA(
-                reads,
-                ch_starindex_ref,
-                ch_gtf,
-                star_ignore_sjdbgtf,
-                '',
-                seq_center
+        STAR_FOR_ARRIBA(
+            reads,
+            ch_starindex_ref,
+            ch_gtf,
+            star_ignore_sjdbgtf,
+            '',
+            seq_center
+        )
+        ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
+
+        if ( tools.contains("ctatsplicing") ) {
+            CTATSPLICING_WORKFLOW(
+                STAR_FOR_ARRIBA.out.spl_junc_tab,
+                STAR_FOR_ARRIBA.out.junction,
+                STAR_FOR_ARRIBA.out.bam,
+                ch_starfusion_ref
             )
-            ch_versions = ch_versions.mix(STAR_FOR_ARRIBA.out.versions)
-
-            if ( ctatsplicing || all ) {
-                CTATSPLICING_WORKFLOW(
-                    STAR_FOR_ARRIBA.out.spl_junc_tab,
-                    STAR_FOR_ARRIBA.out.junction,
-                    STAR_FOR_ARRIBA.out.bam,
-                    ch_starfusion_ref
-                )
-                ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
-            }
-
-            if ( arriba_fusions ) {
-
-                ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
-                    .map { it -> [ it[0], it[2] ] }
-                ch_arriba_fusion_fail = ch_dummy_file
-
-            } else {
+            ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
+        }
 
-                ARRIBA_ARRIBA (
-                    STAR_FOR_ARRIBA.out.bam,
-                    ch_fasta,
-                    ch_gtf,
-                    ch_arriba_ref_blacklist,
-                    ch_arriba_ref_known_fusions,
-                    ch_arriba_ref_cytobands,
-                    ch_arriba_ref_protein_domains
-                )
+        if ( arriba_fusions ) {
 
-                ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
+            ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
+                .map { it -> [ it[0], it[2] ] }
+            ch_arriba_fusion_fail = ch_dummy_file
 
-                ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
-                ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ it -> return it[1] }
-            }
+        } else {
 
-            if ( cram.contains('arriba') ) {
+            ARRIBA_ARRIBA (
+                STAR_FOR_ARRIBA.out.bam,
+                ch_fasta,
+                ch_gtf,
+                ch_arriba_ref_blacklist,
+                ch_arriba_ref_known_fusions,
+                ch_arriba_ref_cytobands,
+                ch_arriba_ref_protein_domains
+            )
 
-                SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam, ch_fasta)
-                ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions )
+            ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
-                SAMTOOLS_VIEW_FOR_ARRIBA(SAMTOOLS_SORT_FOR_ARRIBA.out.bam.map { meta, bam -> [ meta, bam, [] ] }, ch_fasta, [])
-                ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_ARRIBA.out.versions )
+            ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
+            ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ it -> return it[1] }
+        }
 
-                SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_VIEW_FOR_ARRIBA.out.cram)
-                ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions )
+        if ( cram.contains('arriba') ) {
 
-                // Join cram and index files
-                ch_cram_index = SAMTOOLS_VIEW_FOR_ARRIBA.out.cram.join(SAMTOOLS_INDEX_FOR_ARRIBA.out.crai)
-            }
+            SAMTOOLS_SORT_FOR_ARRIBA(STAR_FOR_ARRIBA.out.bam, ch_fasta)
+            ch_versions = ch_versions.mix(SAMTOOLS_SORT_FOR_ARRIBA.out.versions )
 
-        } else {
+            SAMTOOLS_VIEW_FOR_ARRIBA(SAMTOOLS_SORT_FOR_ARRIBA.out.bam.map { meta, bam -> [ meta, bam, [] ] }, ch_fasta, [])
+            ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_ARRIBA.out.versions )
 
-            ch_arriba_fusions = reads
-                .combine(Channel.value( file(ch_dummy_file, checkIfExists: true ) ) )
-                .map { it -> [ it[0], it[2] ] }
+            SAMTOOLS_INDEX_FOR_ARRIBA(SAMTOOLS_VIEW_FOR_ARRIBA.out.cram)
+            ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_ARRIBA.out.versions )
 
-            ch_arriba_fusion_fail = ch_dummy_file
+            // Join cram and index files
+            ch_cram_index = SAMTOOLS_VIEW_FOR_ARRIBA.out.cram.join(SAMTOOLS_INDEX_FOR_ARRIBA.out.crai)
         }
 
     emit:
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 48bb147f0..e827b9851 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -85,27 +85,25 @@ workflow RNAFUSION {
         // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
         // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
 
-        ARRIBA_WORKFLOW (
-            ch_reads,
-            BUILD_REFERENCES.out.ch_gtf,
-            BUILD_REFERENCES.out.ch_fasta,
-            BUILD_REFERENCES.out.ch_starindex_ref,
-            BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
-            BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
-            BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
-            BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
-            BUILD_REFERENCES.out.ch_starfusion_ref,
-            params.arriba,                   // boolean
-            params.all,                      // boolean
-            params.fusioninspector_only,     // boolean
-            params.star_ignore_sjdbgtf,      // boolean
-            params.ctatsplicing,             // boolean
-            params.seq_center ?: '',         // string
-            params.arriba_fusions,           // path
-            params.cram                      // array
-        )
-        ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
-
+        if(tools.intersect(["arriba", "ctatsplicing"])) {
+            ARRIBA_WORKFLOW (
+                ch_reads,
+                BUILD_REFERENCES.out.ch_gtf,
+                BUILD_REFERENCES.out.ch_fasta,
+                BUILD_REFERENCES.out.ch_starindex_ref,
+                BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
+                BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
+                BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
+                BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
+                BUILD_REFERENCES.out.ch_starfusion_ref,
+                tools,
+                params.star_ignore_sjdbgtf,      // boolean
+                params.seq_center ?: '',         // string
+                params.arriba_fusions,           // path
+                params.cram                      // array
+            )
+            ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
+        }
 
         //Run STAR fusion
         STARFUSION_WORKFLOW (

From 4b15bf0b5810240788512d41a4e51a50de3d00a7 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 16:49:11 +0200
Subject: [PATCH 368/887] fusioncatcher and starfusion

---
 subworkflows/local/arriba_workflow/main.nf    |  2 -
 .../local/fusioncatcher_workflow/main.nf      | 34 +++-----
 .../local/starfusion_workflow/main.nf         | 79 +++++++++----------
 subworkflows/local/stringtie_workflow.nf      | 30 ++++---
 workflows/rnafusion.nf                        | 66 +++++++++-------
 5 files changed, 101 insertions(+), 110 deletions(-)

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 0c46608f7..447b57c1a 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -27,7 +27,6 @@ workflow ARRIBA_WORKFLOW {
 
         def ch_versions   = Channel.empty()
         def ch_cram_index = Channel.empty()
-        def ch_dummy_file = file("$projectDir/assets/dummy_file_arriba.txt", checkIfExists: true)
 
         STAR_FOR_ARRIBA(
             reads,
@@ -53,7 +52,6 @@ workflow ARRIBA_WORKFLOW {
 
             ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
                 .map { it -> [ it[0], it[2] ] }
-            ch_arriba_fusion_fail = ch_dummy_file
 
         } else {
 
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index 35e74030c..f500bc333 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -9,33 +9,23 @@ workflow FUSIONCATCHER_WORKFLOW {
     take:
         reads                   // channel [ meta, [ fastqs ] ]
         fusioncatcher_ref       // channel [ meta, path       ]
-        fusioncatcher          // boolean
-        all                     // boolean
-        fusioninspector_only    // boolean
         fusioncatcher_fusions   // path, string
 
     main:
         ch_versions   = Channel.empty()
-        ch_dummy_file = file("$baseDir/assets/dummy_file_fusioncatcher.txt", checkIfExists: true)
-
-        if (( fusioncatcher || all) && !fusioninspector_only ) {
-            if (fusioncatcher_fusions){
-
-                ch_fusioncatcher_fusions = reads.combine(Channel.value(file(fusioncatcher_fusions, checkIfExists:true)))
-                                            .map { meta, _reads, fusions -> [ meta, fusions ] }
-            } else {
-
-                FUSIONCATCHER_DETECT (
-                    reads,
-                    fusioncatcher_ref
-                )
-                ch_fusioncatcher_fusions = FUSIONCATCHER_DETECT.out.fusions
-                ch_versions              = ch_versions.mix(FUSIONCATCHER_DETECT.out.versions)
-            }
-        }
-        else {
-            ch_fusioncatcher_fusions = reads.combine(Channel.value(file(ch_dummy_file, checkIfExists:true)))
+
+        if (fusioncatcher_fusions){
+
+            ch_fusioncatcher_fusions = reads.combine(Channel.value(file(fusioncatcher_fusions, checkIfExists:true)))
                                         .map { meta, _reads, fusions -> [ meta, fusions ] }
+        } else {
+
+            FUSIONCATCHER_DETECT (
+                reads,
+                fusioncatcher_ref
+            )
+            ch_fusioncatcher_fusions = FUSIONCATCHER_DETECT.out.fusions
+            ch_versions              = ch_versions.mix(FUSIONCATCHER_DETECT.out.versions)
         }
 
     emit:
diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index 938832c96..d4bee449b 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -12,6 +12,7 @@ workflow STARFUSION_WORKFLOW {
         ch_starindex_ref
         ch_fasta
         ch_starfusion_ref
+        tools
 
     main:
         def ch_versions = Channel.empty()
@@ -19,56 +20,48 @@ workflow STARFUSION_WORKFLOW {
         def ch_starfusion_fusions = Channel.empty()
         def bam_sorted_indexed = Channel.empty()
 
-        ch_dummy_file = file("$baseDir/assets/dummy_file_starfusion.txt", checkIfExists: true)
-
-        if ((params.starfusion || params.all || params.stringtie) && !params.fusioninspector_only) {
-            if (params.starfusion_fusions){
-                ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
-                                        .map { it -> [ it[0], it[2] ] }
-            } else {
-                STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
-                ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
-                ch_align = STAR_FOR_STARFUSION.out.bam_sorted // TODO: This does not seem to be captured and used as the output is bam_sorted_aligned and not bam_sorted
-
+        if (params.starfusion_fusions){
+            ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
+                                    .map { it -> [ it[0], it[2] ] }
+        } else {
+            STAR_FOR_STARFUSION( reads, ch_starindex_ref, ch_gtf, params.star_ignore_sjdbgtf, '', params.seq_center ?: '')
+            ch_versions = ch_versions.mix(STAR_FOR_STARFUSION.out.versions)
+            ch_align = STAR_FOR_STARFUSION.out.bam_sorted // TODO: This does not seem to be captured and used as the output is bam_sorted_aligned and not bam_sorted
 
-                SAMTOOLS_INDEX_FOR_STARFUSION(STAR_FOR_STARFUSION.out.bam_sorted)
-                ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION.out.versions)
-                bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai)
-                reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )  // TODO: This join is not needed as STARFUSION can simply read from the junction file: https://github.com/STAR-Fusion/STAR-Fusion/wiki#alternatively-kickstart-mode-running-star-yourself-and-then-running-star-fusion-using-the-existing-outputs
 
-                if (params.ctatsplicing || params.all) {
-                    CTATSPLICING_WORKFLOW(
-                        STAR_FOR_STARFUSION.out.spl_junc_tab,
-                        STAR_FOR_STARFUSION.out.junction,
-                        STAR_FOR_STARFUSION.out.bam,
-                        ch_starfusion_ref
-                    )
-                    ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
-                }
+            SAMTOOLS_INDEX_FOR_STARFUSION(STAR_FOR_STARFUSION.out.bam_sorted)
+            ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION.out.versions)
+            bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai)
+            reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction )  // TODO: This join is not needed as STARFUSION can simply read from the junction file: https://github.com/STAR-Fusion/STAR-Fusion/wiki#alternatively-kickstart-mode-running-star-yourself-and-then-running-star-fusion-using-the-existing-outputs
 
-                if (params.cram.contains('starfusion')){
-                    SAMTOOLS_VIEW_FOR_STARFUSION (bam_sorted_indexed, ch_fasta, [] )
-                    ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_STARFUSION.out.versions)
+            if (tools.contains("ctatsplicing")) {
+                CTATSPLICING_WORKFLOW(
+                    STAR_FOR_STARFUSION.out.spl_junc_tab,
+                    STAR_FOR_STARFUSION.out.junction,
+                    STAR_FOR_STARFUSION.out.bam,
+                    ch_starfusion_ref
+                )
+                ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
+            }
 
-                    SAMTOOLS_INDEX_FOR_STARFUSION_CRAM (SAMTOOLS_VIEW_FOR_STARFUSION.out.cram)
-                    ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION_CRAM.out.versions)
-                }
-                if (params.starfusion || params.all){
-                    STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
-                    ch_versions = ch_versions.mix(STARFUSION.out.versions)
-                    ch_starfusion_fusions = STARFUSION.out.fusions
-                }
+            if (params.cram.contains('starfusion')){
+                SAMTOOLS_VIEW_FOR_STARFUSION (bam_sorted_indexed, ch_fasta, [] )
+                ch_versions = ch_versions.mix(SAMTOOLS_VIEW_FOR_STARFUSION.out.versions)
 
-                ch_star_stats = STAR_FOR_STARFUSION.out.log_final
-                ch_star_gene_count = STAR_FOR_STARFUSION.out.read_per_gene_tab
+                SAMTOOLS_INDEX_FOR_STARFUSION_CRAM (SAMTOOLS_VIEW_FOR_STARFUSION.out.cram)
+                ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION_CRAM.out.versions)
             }
+            if (params.starfusion || params.all){
+                STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
+                ch_versions = ch_versions.mix(STARFUSION.out.versions)
+                ch_starfusion_fusions = STARFUSION.out.fusions
+            }
+
+            ch_star_stats = STAR_FOR_STARFUSION.out.log_final
+            ch_star_gene_count = STAR_FOR_STARFUSION.out.read_per_gene_tab
         }
-        else {
-            ch_starfusion_fusions = reads.combine(Channel.value(file(ch_dummy_file, checkIfExists:true)))
-                                    .map { it -> [ it[0], it[2] ] }
-            ch_star_stats = Channel.empty()
-            ch_star_gene_count = Channel.empty()
-        }
+
+
     emit:
         fusions               = ch_starfusion_fusions
         star_stats            = ch_star_stats
diff --git a/subworkflows/local/stringtie_workflow.nf b/subworkflows/local/stringtie_workflow.nf
index d66ec0ee4..a9e05f304 100644
--- a/subworkflows/local/stringtie_workflow.nf
+++ b/subworkflows/local/stringtie_workflow.nf
@@ -11,22 +11,20 @@ workflow STRINGTIE_WORKFLOW {
         ch_versions = Channel.empty()
         ch_stringtie_gtf = Channel.empty()
 
-        if ((params.stringtie || params.all) && !params.fusioninspector_only) {
-            STRINGTIE_STRINGTIE(bam_sorted, ch_gtf.map { meta, gtf -> [ gtf ]})
-            ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-            STRINGTIE_STRINGTIE
-                .out
-                .transcript_gtf
-                .map { it -> it[1] }
-                .set { stringtie_gtf }
-            ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-
-            STRINGTIE_MERGE (stringtie_gtf, ch_gtf.map { meta, gtf -> [ gtf ]})
-            ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
-            ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
-        }
+        STRINGTIE_STRINGTIE(bam_sorted, ch_gtf.map { _meta, gtf -> [ gtf ]})
+        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
+
+        STRINGTIE_STRINGTIE
+            .out
+            .transcript_gtf
+            .map { it -> it[1] }
+            .set { stringtie_gtf }
+        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
+
+
+        STRINGTIE_MERGE (stringtie_gtf, ch_gtf.map { _meta, gtf -> [ gtf ]})
+        ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
+        ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
 
     emit:
         stringtie_gtf = ch_stringtie_gtf.ifEmpty(null)
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index e827b9851..db20d3695 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -78,7 +78,7 @@ workflow RNAFUSION {
 
 
         //
-        // SUBWORKFLOW:  Run STAR alignment and Arriba
+        // SUBWORKFLOW: Run STAR alignment and Arriba
         //
 
         // TODO: add params.seq_platform and pass it as argument to arriba_workflow
@@ -105,39 +105,51 @@ workflow RNAFUSION {
             ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
         }
 
-        //Run STAR fusion
-        STARFUSION_WORKFLOW (
-            ch_reads,
-            BUILD_REFERENCES.out.ch_gtf,
-            BUILD_REFERENCES.out.ch_starindex_ref,
-            BUILD_REFERENCES.out.ch_fasta,
-            BUILD_REFERENCES.out.ch_starfusion_ref
-        )
-        ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+        //
+        // SUBWORKFLOW: Run STAR alignment and StarFusion
+        //
 
+        if(tools.intersect(["starfusion", "ctatsplicing", "stringtie"])) {
+            STARFUSION_WORKFLOW (
+                ch_reads,
+                BUILD_REFERENCES.out.ch_gtf,
+                BUILD_REFERENCES.out.ch_starindex_ref,
+                BUILD_REFERENCES.out.ch_fasta,
+                BUILD_REFERENCES.out.ch_starfusion_ref,
+                tools
+            )
+            ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+        }
 
+        //
+        // SUBWORKFLOW: Run FusionCatcher
+        //
 
-    //Run fusioncatcher
-    FUSIONCATCHER_WORKFLOW (
-        ch_reads,
-        BUILD_REFERENCES.out.ch_fusioncatcher_ref,       // channel [ meta, path       ]
-        params.fusioncatcher,
-        params.all,
-        params.fusioninspector_only,
-        params.fusioncatcher_fusions
-    )
-    ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+        if(tools.contains("fusioncatcher")) {
+            FUSIONCATCHER_WORKFLOW (
+                ch_reads,
+                BUILD_REFERENCES.out.ch_fusioncatcher_ref,       // channel [ meta, path       ]
+                params.fusioncatcher_fusions
+            )
+            ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+        }
 
+        //
+        // SUBWORKFLOW: Run Stringtie
+        //
 
-        //Run stringtie
-        STRINGTIE_WORKFLOW (
-            STARFUSION_WORKFLOW.out.ch_bam_sorted,
-            BUILD_REFERENCES.out.ch_gtf
-        )
-        ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
+        if(tools.contains("stringtie")) {
+            STRINGTIE_WORKFLOW (
+                STARFUSION_WORKFLOW.out.ch_bam_sorted,
+                BUILD_REFERENCES.out.ch_gtf
+            )
+            ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
+        }
 
+        //
+        // SUBWORKFLOW: Run FusionReport
+        //
 
-        //Run fusion-report
         FUSIONREPORT_WORKFLOW (
             ch_reads,
             BUILD_REFERENCES.out.ch_fusionreport_ref,

From 1e505f79004ae4e87d96f6882311bc73f3f77256 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 17:21:39 +0200
Subject: [PATCH 369/887] fusioninspector flow

---
 main.nf                                       |  3 +-
 nextflow.config                               |  1 -
 nextflow_schema.json                          |  5 --
 .../local/fusioninspector_workflow.nf         |  3 +-
 .../local/fusionreport_workflow/main.nf       | 25 +++-----
 workflows/rnafusion.nf                        | 61 +++++++++++++------
 6 files changed, 56 insertions(+), 42 deletions(-)

diff --git a/main.nf b/main.nf
index c2f826aff..29708e5a4 100644
--- a/main.nf
+++ b/main.nf
@@ -47,8 +47,7 @@ workflow {
         "starindex",
         "starfusion",
         "stringtie",
-        "fusion_report",
-        "fusioninspector"
+        "fusion_report"
     ]
 
     def tools = params.all ? all_tools : all_tools.findAll { tool ->
diff --git a/nextflow.config b/nextflow.config
index e8067f759..aac49eecb 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -62,7 +62,6 @@ params {
     stringtie                  = false
     fusionreport               = false
     fusioninspector_only       = false
-    fusioninspector            = false
 
     // Skip steps
     skip_qc                    = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 297f64055..7f8ed19ae 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -198,11 +198,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Skip fusion-report. --fusioninspector_fusions PATH needed to provide a fusion list as input"
                 },
-                "fusioninspector": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Run fusioninspector"
-                },
                 "fusioninspector_fusions": {
                     "type": "string",
                     "format": "file-path",
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index f521ae8cb..a2c207ca0 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -17,6 +17,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         ch_arriba_ref_cytobands
         ch_hgnc_ref
         ch_hgnc_date
+        skip_vis
 
     main:
         ch_versions = Channel.empty()
@@ -51,7 +52,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
         ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)
 
-        if ((params.starfusion || params.all || params.stringtie) && !params.fusioninspector_only && !params.skip_vis) {
+        if (!skip_vis) {
             ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(FUSIONINSPECTOR.out.tsv)
             ARRIBA_VISUALISATION(ch_bam_sorted_indexed_fusions, ch_gtf, ch_arriba_ref_protein_domains, ch_arriba_ref_cytobands)
             ch_versions = ch_versions.mix(ARRIBA_VISUALISATION.out.versions)
diff --git a/subworkflows/local/fusionreport_workflow/main.nf b/subworkflows/local/fusionreport_workflow/main.nf
index eac40e328..bbbc909fb 100644
--- a/subworkflows/local/fusionreport_workflow/main.nf
+++ b/subworkflows/local/fusionreport_workflow/main.nf
@@ -14,31 +14,24 @@ workflow FUSIONREPORT_WORKFLOW {
         ch_report = Channel.empty()
         ch_csv = Channel.empty()
 
-        if (!params.fusioninspector_only) {
-            reads_fusions = reads
+        reads_fusions = reads
             .join(arriba_fusions, failOnMismatch:true, failOnDuplicate:true)
             .join(starfusion_fusions, failOnMismatch:true, failOnDuplicate:true)
             .join(fusioncatcher_fusions, failOnMismatch:true, failOnDuplicate:true)
 
-            FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
-            ch_fusion_list = FUSIONREPORT.out.fusion_list
-            ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered
-            ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
-            ch_report = FUSIONREPORT.out.report
-            ch_csv = FUSIONREPORT.out.csv
-        } else {
-            ch_fusion_list = reads.combine(Channel.value(file(params.fusioninspector_fusions, checkIfExists:true)))
-                            .map { it -> [ it[0], it[1] ] }
-
-            ch_fusion_list_filtered  = ch_fusion_list
-        }
+        FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
+        ch_fusion_list = FUSIONREPORT.out.fusion_list
+        ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered
+        ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
+        ch_report = FUSIONREPORT.out.report
+        ch_csv = FUSIONREPORT.out.csv
 
     emit:
         versions                 = ch_versions
         fusion_list              = ch_fusion_list
         fusion_list_filtered     = ch_fusion_list_filtered
-        report                   = ch_report.ifEmpty(null)
-        csv                      = ch_csv.ifEmpty(null)
+        report                   = ch_report
+        csv                      = ch_csv
 
 }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index db20d3695..61ec12dcd 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -22,6 +22,8 @@ include { paramsSummaryMultiqc          }   from '../subworkflows/nf-core/utils_
 include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
+include { ARRIBA_ARRIBA } from '../modules/nf-core/arriba/arriba/main.nf'
+include { FUSIONREPORT } from '../modules/local/fusionreport/detect/main.nf'
 
 
 /*
@@ -69,7 +71,7 @@ workflow RNAFUSION {
             Channel.value(params.adapter_fasta),
             params.fastp_trim
         )
-        ch_reads = TRIM_WORKFLOW.out.ch_reads_all
+        def ch_reads = TRIM_WORKFLOW.out.ch_reads_all
         ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
 
         SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
@@ -85,6 +87,7 @@ workflow RNAFUSION {
         // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
         // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
 
+        def ch_arriba_fusions = ch_reads.map { it -> [it[0], []] } // Set arriba fusions to empty by default
         if(tools.intersect(["arriba", "ctatsplicing"])) {
             ARRIBA_WORKFLOW (
                 ch_reads,
@@ -103,12 +106,15 @@ workflow RNAFUSION {
                 params.cram                      // array
             )
             ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
+            ch_arriba_fusions = ARRIBA_WORKFLOW.out.fusions
         }
 
         //
         // SUBWORKFLOW: Run STAR alignment and StarFusion
         //
 
+        def ch_starfusion_fusions = ch_reads.map { it -> [it[0], []] } // Set starfusion fusions to empty by default
+        def ch_starfusion_bams = Channel.empty()
         if(tools.intersect(["starfusion", "ctatsplicing", "stringtie"])) {
             STARFUSION_WORKFLOW (
                 ch_reads,
@@ -119,12 +125,15 @@ workflow RNAFUSION {
                 tools
             )
             ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+            ch_starfusion_fusions = STARFUSION_WORKFLOW.out.fusions
+            ch_starfusion_bams = STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed
         }
 
         //
         // SUBWORKFLOW: Run FusionCatcher
         //
 
+        def ch_fusioncatcher_fusions = ch_reads.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
             FUSIONCATCHER_WORKFLOW (
                 ch_reads,
@@ -150,32 +159,50 @@ workflow RNAFUSION {
         // SUBWORKFLOW: Run FusionReport
         //
 
-        FUSIONREPORT_WORKFLOW (
-            ch_reads,
-            BUILD_REFERENCES.out.ch_fusionreport_ref,
-            ARRIBA_WORKFLOW.out.fusions,
-            STARFUSION_WORKFLOW.out.fusions,
-            FUSIONCATCHER_WORKFLOW.out.fusions
-        )
-        ch_versions = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
+        def ch_fusion_list = Channel.empty()
+        def ch_fusion_list_filtered = Channel.empty()
+        def ch_fusionreport_report = Channel.empty()
+        def ch_fusionreport_csv = Channel.empty()
+        if(tools.intersect(["arriba", "starfusion", "fusioncatcher"])) {
+            FUSIONREPORT_WORKFLOW (
+                ch_reads,
+                BUILD_REFERENCES.out.ch_fusionreport_ref,
+                ch_arriba_fusions,
+                ch_starfusion_fusions,
+                ch_fusioncatcher_fusions
+            )
+            ch_versions             = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
+            ch_fusion_list          = FUSIONREPORT_WORKFLOW.out.fusion_list
+            ch_fusion_list_filtered = FUSIONREPORT_WORKFLOW.out.fusion_list_filtered
+            ch_fusionreport_report  = FUSIONREPORT_WORKFLOW.out.report
+            ch_fusionreport_csv     = FUSIONREPORT_WORKFLOW.out.csv
+        } else if(params.fusioninspector_fusions) {
+            def input_fusions = file(params.fusioninspector_fusions, checkIfExists:true)
+            ch_fusion_list = ch_reads.map { it -> [ it[0], input_fusions ] }
+        } else {
+            error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with --arriba, --starfusion and/or --fusioncatcher")
+        }
+
+        //
+        // SUBWORKFLOW: Run FusionInspector
+        //
 
-        //Run fusionInpector
         FUSIONINSPECTOR_WORKFLOW (
             ch_reads,
-            FUSIONREPORT_WORKFLOW.out.fusion_list,
-            FUSIONREPORT_WORKFLOW.out.fusion_list_filtered,
-            FUSIONREPORT_WORKFLOW.out.report,
-            FUSIONREPORT_WORKFLOW.out.csv,
-            STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed,
+            ch_fusion_list,
+            ch_fusion_list_filtered,
+            ch_fusionreport_report,
+            ch_fusionreport_csv,
+            ch_starfusion_bams,
             BUILD_REFERENCES.out.ch_gtf,
             BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
             BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
             BUILD_REFERENCES.out.ch_hgnc_ref,
-            BUILD_REFERENCES.out.ch_hgnc_date
+            BUILD_REFERENCES.out.ch_hgnc_date,
+            params.skip_vis
         )
         ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
 
-
         //QC
         QC_WORKFLOW (
             STARFUSION_WORKFLOW.out.ch_bam_sorted,

From 69ba70d04b64fb75bb8353489bf2958cdddd460d Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 17:28:12 +0200
Subject: [PATCH 370/887] qc logic

---
 workflows/rnafusion.nf | 62 ++++++++++++++++++++----------------------
 1 file changed, 30 insertions(+), 32 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 61ec12dcd..eb9fa0bd1 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -71,8 +71,11 @@ workflow RNAFUSION {
             Channel.value(params.adapter_fasta),
             params.fastp_trim
         )
-        def ch_reads = TRIM_WORKFLOW.out.ch_reads_all
-        ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+        def ch_reads     = TRIM_WORKFLOW.out.ch_reads_all
+        ch_versions      = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+        ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]})
+        ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]})
+        ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]})
 
         SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
         ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
@@ -124,9 +127,11 @@ workflow RNAFUSION {
                 BUILD_REFERENCES.out.ch_starfusion_ref,
                 tools
             )
-            ch_versions = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
-            ch_starfusion_fusions = STARFUSION_WORKFLOW.out.fusions
-            ch_starfusion_bams = STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed
+            ch_versions             = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
+            ch_starfusion_fusions   = STARFUSION_WORKFLOW.out.fusions
+            ch_starfusion_bams      = STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed
+            ch_multiqc_files        = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]})
+            ch_multiqc_files        = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]})
         }
 
         //
@@ -178,7 +183,7 @@ workflow RNAFUSION {
             ch_fusionreport_csv     = FUSIONREPORT_WORKFLOW.out.csv
         } else if(params.fusioninspector_fusions) {
             def input_fusions = file(params.fusioninspector_fusions, checkIfExists:true)
-            ch_fusion_list = ch_reads.map { it -> [ it[0], input_fusions ] }
+            ch_fusion_list    = ch_reads.map { it -> [ it[0], input_fusions ] }
         } else {
             error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with --arriba, --starfusion and/or --fusioncatcher")
         }
@@ -201,18 +206,26 @@ workflow RNAFUSION {
             BUILD_REFERENCES.out.ch_hgnc_date,
             params.skip_vis
         )
-        ch_versions = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
-
-        //QC
-        QC_WORKFLOW (
-            STARFUSION_WORKFLOW.out.ch_bam_sorted,
-            BUILD_REFERENCES.out.ch_refflat,
-            BUILD_REFERENCES.out.ch_fasta,
-            BUILD_REFERENCES.out.ch_fai,
-            BUILD_REFERENCES.out.ch_rrna_interval
-        )
-        ch_versions = ch_versions.mix(QC_WORKFLOW.out.versions)
+        ch_versions      = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
+        ch_multiqc_files = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
+
+        //
+        // SUBWORKFLOW: Run QC
+        //
 
+        if(!params.skip_qc) {
+            QC_WORKFLOW (
+                STARFUSION_WORKFLOW.out.ch_bam_sorted,
+                BUILD_REFERENCES.out.ch_refflat,
+                BUILD_REFERENCES.out.ch_fasta,
+                BUILD_REFERENCES.out.ch_fai,
+                BUILD_REFERENCES.out.ch_rrna_interval
+            )
+            ch_versions      = ch_versions.mix(QC_WORKFLOW.out.versions)
+            ch_multiqc_files = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]})
+            ch_multiqc_files = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]})
+            ch_multiqc_files = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]})
+        }
     }
     //
     // Collate and save software versions
@@ -257,19 +270,6 @@ workflow RNAFUSION {
         )
     )
 
-    if (!params.references_only) { // TODO: Remove this temporary parameter when we have a full-working GitHub nf-test
-        ch_multiqc_files                      = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.duplicate_metrics.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(QC_WORKFLOW.out.insertsize_metrics.collect{it[1]}.ifEmpty([]))
-        ch_multiqc_files                      = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
-    }
-
     MULTIQC (
         ch_multiqc_files.collect(),
         ch_multiqc_config.toList(),
@@ -279,8 +279,6 @@ workflow RNAFUSION {
         []
     )
 
-
-
     emit:
     multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
     versions       = ch_versions                 // channel: [ path(versions.yml) ]

From 0fa91fcf0d85c56eb1fcc6f86201e39640f37893 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 17:36:52 +0200
Subject: [PATCH 371/887] trim workflow + first batch of ext.when removals

---
 conf/modules.config                      |  2 --
 main.nf                                  |  3 +-
 nextflow.config                          |  5 ++-
 nextflow_schema.json                     |  7 +----
 subworkflows/local/trim_workflow/main.nf | 27 +++++++---------
 workflows/rnafusion.nf                   | 39 ++++++++++++++----------
 6 files changed, 39 insertions(+), 44 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 0fc0be935..f3d619d16 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -36,7 +36,6 @@ process {
     }
 
     withName: 'ARRIBA_VISUALISATION' {
-        ext.when   = { {!params.fusioninspector_only} && ({params.starfusion} || {params.all}) }
         ext.prefix = { "${meta.id}_combined_fusions_arriba_visualisation" }
         publishDir = [
             path: { "${params.outdir}/arriba_visualisation" },
@@ -83,7 +82,6 @@ process {
 
     withName: 'FASTQC' {
         ext.args = '--quiet'
-        ext.when = {!params.skip_qc}
         publishDir = [
             path: { "${params.outdir}/fastqc" },
             mode: params.publish_dir_mode,
diff --git a/main.nf b/main.nf
index 29708e5a4..63870ba33 100644
--- a/main.nf
+++ b/main.nf
@@ -47,7 +47,8 @@ workflow {
         "starindex",
         "starfusion",
         "stringtie",
-        "fusion_report"
+        "fusion_report",
+        "fastp"
     ]
 
     def tools = params.all ? all_tools : all_tools.findAll { tool ->
diff --git a/nextflow.config b/nextflow.config
index aac49eecb..5d45390f1 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -38,7 +38,6 @@ params {
     tools_cutoff               = 1
 
     // Trimming
-    fastp_trim                 = false
     trim_tail                  = null
     adapter_fasta              = []
 
@@ -52,7 +51,7 @@ params {
     fusioncatcher_limitSjdbInsertNsj     = 2000000
     fusioninspector_limitSjdbInsertNsj   = 1000000
 
-    // Enable or disable tools
+    // Enable tools
     all                        = false
     arriba                     = false
     ctatsplicing               = false
@@ -61,7 +60,7 @@ params {
     starfusion                 = false
     stringtie                  = false
     fusionreport               = false
-    fusioninspector_only       = false
+    fastp                      = false
 
     // Skip steps
     skip_qc                    = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 7f8ed19ae..26585381f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -193,11 +193,6 @@
                     "default": 1000000,
                     "minimum": 1
                 },
-                "fusioninspector_only": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Skip fusion-report. --fusioninspector_fusions PATH needed to provide a fusion list as input"
-                },
                 "fusioninspector_fusions": {
                     "type": "string",
                     "format": "file-path",
@@ -332,7 +327,7 @@
             "fa_icon": "fas fa-cut",
             "description": "Options to adjust read trimming criteria.",
             "properties": {
-                "fastp_trim": {
+                "fastp": {
                     "type": "boolean",
                     "description": "Preform fastp trimming of reads, default: false",
                     "fa_icon": "fas fa-cut"
diff --git a/subworkflows/local/trim_workflow/main.nf b/subworkflows/local/trim_workflow/main.nf
index d548c8aec..d4d559f79 100644
--- a/subworkflows/local/trim_workflow/main.nf
+++ b/subworkflows/local/trim_workflow/main.nf
@@ -8,7 +8,7 @@ workflow TRIM_WORKFLOW {
     take:
         reads           // channel [ meta, [ fastq files ] ]
         adapter_fasta   // channel [ path ]
-        fastp_trim      // boolean
+        skip_qc         // boolean
 
     main:
         ch_versions       = Channel.empty()
@@ -16,25 +16,20 @@ workflow TRIM_WORKFLOW {
         ch_fastp_json     = Channel.empty()
         ch_fastqc_trimmed = Channel.empty()
 
-        if ( fastp_trim ) {
-            FASTP(reads, adapter_fasta.ifEmpty( [] ), false, false, false)
-            ch_versions = ch_versions.mix(FASTP.out.versions)
+        FASTP(reads, adapter_fasta, false, false, false)
+        ch_versions = ch_versions.mix(FASTP.out.versions)
 
+        if(!skip_qc) {
             FASTQC_FOR_FASTP(FASTP.out.reads)
-            ch_versions = ch_versions.mix(FASTQC_FOR_FASTP.out.versions)
-
-            ch_reads_all           = FASTP.out.reads
-            ch_reads_fusioncatcher = ch_reads_all
-            ch_fastp_html          = FASTP.out.html
-            ch_fastp_json          = FASTP.out.json
-            ch_fastqc_trimmed      = FASTQC_FOR_FASTP.out.zip
-
-        }
-        else {
-            ch_reads_all           = reads
-            ch_reads_fusioncatcher = reads
+            ch_versions       = ch_versions.mix(FASTQC_FOR_FASTP.out.versions)
+            ch_fastqc_trimmed = FASTQC_FOR_FASTP.out.zip
         }
 
+        ch_reads_all           = FASTP.out.reads
+        ch_reads_fusioncatcher = ch_reads_all
+        ch_fastp_html          = FASTP.out.html
+        ch_fastp_json          = FASTP.out.json
+
     emit:
         ch_reads_all            // Channel [ meta, [reads]   ]
         ch_reads_fusioncatcher  // Channel [ meta, [reads]   ]
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index eb9fa0bd1..9ecb45c83 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -56,26 +56,33 @@ workflow RNAFUSION {
         //
         // QC from FASTQ files
         //
-        FASTQC (
-            ch_samplesheet
-        )
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-        ch_versions = ch_versions.mix(FASTQC.out.versions)
 
+        if(!params.skip_qc) {
+            FASTQC (
+                ch_samplesheet
+            )
+            ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
+            ch_versions = ch_versions.mix(FASTQC.out.versions)
+        }
 
         //
-        // Trimming
+        // SUBWORKFLOW: Trimming
         //
-        TRIM_WORKFLOW (
-            ch_samplesheet,
-            Channel.value(params.adapter_fasta),
-            params.fastp_trim
-        )
-        def ch_reads     = TRIM_WORKFLOW.out.ch_reads_all
-        ch_versions      = ch_versions.mix(TRIM_WORKFLOW.out.versions)
-        ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]})
-        ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]})
-        ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]})
+
+        def ch_reads = ch_samplesheet // Use non-trimmed reads by default
+        if(tools.contains("fastp")) {
+            def ch_adapter_fasta = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta).collect() : []
+            TRIM_WORKFLOW (
+                ch_samplesheet,
+                ch_adapter_fasta,
+                params.skip_qc
+            )
+            ch_reads = TRIM_WORKFLOW.out.ch_reads_all
+            ch_versions      = ch_versions.mix(TRIM_WORKFLOW.out.versions)
+            ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]})
+            ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]})
+            ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]})
+        }
 
         SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
         ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})

From 76b329e6bb5334fafd08fd8957fa77374a3668ad Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 17:45:30 +0200
Subject: [PATCH 372/887] update changelog + add salmon param

---
 CHANGELOG.md                           |  3 +++
 conf/modules.config                    |  1 -
 main.nf                                |  3 ++-
 nextflow_schema.json                   |  5 +++++
 subworkflows/local/build_references.nf |  3 ++-
 workflows/rnafusion.nf                 | 13 ++++++++-----
 6 files changed, 20 insertions(+), 8 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 41fc439ff..687ec63d3 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,6 +31,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `CTATSPLICING_PREPGENOMELIB` to update the starfusion genome library directory with a cancer splicing index. [#610](https://github.com/nf-core/rnafusion/pull/610)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
 - Add nf-test to local module: `ARRIBA_VISUALISATION`. [#625](https://github.com/nf-core/rnafusion/pull/625)
+- Added a new parameter `salmon` to enable the Salmon tool (default is `false`) [#]()
 
 ### Changed
 
@@ -51,6 +52,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
 - Update htslib and samtools version in `star/align` to 1.21 [#634](https://github.com/nf-core/rnafusion/pull/634)
 - `--run_fusioncatcher` back to `fusioncatcher` [#641](https://github.com/nf-core/rnafusion/pull/641)
+- Removed the `--fusioninspector_only` parameter, this is now the default behaviour of the pipeline [#]()
+- Renamed `--fastp_trim` to `--fastp` to be more uniform across with other tool parameters [#]()
 
 ### Fixed
 
diff --git a/conf/modules.config b/conf/modules.config
index f3d619d16..3bac9ba6b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -91,7 +91,6 @@ process {
 
     withName: 'FASTQC_FOR_FASTP' {
         ext.args = '--quiet'
-        ext.when = { !params.skip_qc }
         ext.prefix = { "${meta.id}_trimmed" }
         publishDir = [
             path: { "${params.outdir}/fastqc_for_fastp" },
diff --git a/main.nf b/main.nf
index 63870ba33..2c3045f45 100644
--- a/main.nf
+++ b/main.nf
@@ -48,7 +48,8 @@ workflow {
         "starfusion",
         "stringtie",
         "fusion_report",
-        "fastp"
+        "fastp",
+        "salmon"
     ]
 
     def tools = params.all ? all_tools : all_tools.findAll { tool ->
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 26585381f..f64cf478c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -237,6 +237,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Use QIAGEN instead of SANGER to download COSMIC database"
                 },
+                "salmon": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Run Salmon"
+                },
                 "salmon_index": {
                     "type": "string",
                     "format": "directory-path",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 0e2963bc5..5d3be6277 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -88,7 +88,8 @@ workflow BUILD_REFERENCES {
         ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
     }
 
-    if (!exists_not_empty(params.salmon_index) || !exists_not_empty(params.salmon_index_stub_check)){ // add condition for qc
+    def run_salmon = tools.contains("salmon")
+    if (run_salmon && (!exists_not_empty(params.salmon_index) || !exists_not_empty(params.salmon_index_stub_check))){ // add condition for qc
         GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
         ch_versions = ch_versions.mix(GFFREAD.out.versions)
         SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 9ecb45c83..a2e609515 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -69,7 +69,7 @@ workflow RNAFUSION {
         // SUBWORKFLOW: Trimming
         //
 
-        def ch_reads = ch_samplesheet // Use non-trimmed reads by default
+        def ch_reads = Channel.empty()
         if(tools.contains("fastp")) {
             def ch_adapter_fasta = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta).collect() : []
             TRIM_WORKFLOW (
@@ -82,12 +82,15 @@ workflow RNAFUSION {
             ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]})
             ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]})
             ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]})
+        } else {
+            ch_reads = ch_samplesheet
         }
 
-        SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
-        ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
-        ch_versions = ch_versions.mix(SALMON_QUANT.out.versions)
-
+        if(tools.contains("salmon")) {
+            SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
+            ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
+            ch_versions      = ch_versions.mix(SALMON_QUANT.out.versions)
+        }
 
         //
         // SUBWORKFLOW: Run STAR alignment and Arriba

From ceb1eb8526a5f4c8f16482e49f0e454aa92dc400 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 17:52:34 +0200
Subject: [PATCH 373/887] remove ext.when

---
 conf/modules.config                           | 18 ----
 .../local/fusioninspector_workflow.nf         |  7 +-
 workflows/rnafusion.nf                        | 89 ++++++++++---------
 3 files changed, 54 insertions(+), 60 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 3bac9ba6b..d69aa7577 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -112,13 +112,11 @@ process {
     }
 
     withName: '.*FUSIONINSPECTOR_WORKFLOW:.*:FUSIONINSPECTOR' {
-        ext.when = { !params.skip_vis }
         ext.args = { ${params.fusioninspector_limitSjdbInsertNsj} != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
         ext.args2 = '--annotate --examine_coding_effect'
     }
 
     withName: 'FUSIONREPORT' {
-        ext.when         = { !params.skip_vis }
         ext.args         = { {params.no_cosmic} ? "--no-cosmic" : "" }
         ext.args2        = "--export csv"
         publishDir = [
@@ -147,7 +145,6 @@ process {
     }
 
     withName: 'GATK4_MARKDUPLICATES' {
-        ext.when         = { {!params.skip_qc} && {!params.fusioninspector_only} && ( {params.starfusion}|| {params.all}) }
         publishDir = [
             path: { "${params.outdir}/picard" },
             mode: params.publish_dir_mode,
@@ -182,7 +179,6 @@ process {
     }
 
     withName: 'MULTIQC' {
-        ext.when         = { !params.skip_qc }
         ext.args   = {params.multiqc_title} ? "--title \"$params.multiqc_title\"" : ''
         publishDir = [
             path: { "${params.outdir}/multiqc" },
@@ -191,13 +187,7 @@ process {
         ]
     }
 
-    withName: 'PICARD_COLLECTRNASEQMETRICS' {
-        ext.when         = { {!params.skip_qc} && {!params.fusioninspector_only} && ( {params.starfusion} || {params.all}) }
-
-    }
-
     withName: 'PICARD_COLLECTINSERTSIZEMETRICS' {
-        ext.when         = { ${!params.skip_qc} && ${!params.fusioninspector_only} && (${params.starfusion} || ${params.all}) }
         ext.prefix       = { "${meta.id}_collectinsertsize"}
         publishDir = [
             path: { "${params.outdir}/picard" },
@@ -388,12 +378,4 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
     }
-
-    withName: 'VCF_COLLECT' {
-        ext.when = { {!params.fusioninspector_only} && {!params.skip_vcf} }
-    }
-
-    withName: '.*' {
-            ext.when = { !params.references_only || task.process.contains('BUILD_REFERENCES') }
-        }
 }
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index a2c207ca0..e17b58974 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -18,6 +18,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         ch_hgnc_ref
         ch_hgnc_date
         skip_vis
+        skip_vcf
 
     main:
         ch_versions = Channel.empty()
@@ -49,8 +50,10 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
         fusion_data = FUSIONINSPECTOR.out.tsv_coding_effect.join(AGAT_CONVERTSPGFF2TSV.out.tsv).join(fusionreport_out).join(fusionreport_csv)
-        VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
-        ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)
+        if(!skip_vcf) {
+            VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
+            ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)
+        }
 
         if (!skip_vis) {
             ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(FUSIONINSPECTOR.out.tsv)
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index a2e609515..b8fa3874f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -178,7 +178,7 @@ workflow RNAFUSION {
         def ch_fusion_list_filtered = Channel.empty()
         def ch_fusionreport_report = Channel.empty()
         def ch_fusionreport_csv = Channel.empty()
-        if(tools.intersect(["arriba", "starfusion", "fusioncatcher"])) {
+        if(tools.intersect(["arriba", "starfusion", "fusioncatcher"]) && !params.skip_vis) {
             FUSIONREPORT_WORKFLOW (
                 ch_reads,
                 BUILD_REFERENCES.out.ch_fusionreport_ref,
@@ -192,10 +192,13 @@ workflow RNAFUSION {
             ch_fusionreport_report  = FUSIONREPORT_WORKFLOW.out.report
             ch_fusionreport_csv     = FUSIONREPORT_WORKFLOW.out.csv
         } else if(params.fusioninspector_fusions) {
-            def input_fusions = file(params.fusioninspector_fusions, checkIfExists:true)
-            ch_fusion_list    = ch_reads.map { it -> [ it[0], input_fusions ] }
+            def input_fusions       = file(params.fusioninspector_fusions, checkIfExists:true)
+            ch_fusion_list          = ch_reads.map { it -> [ it[0], input_fusions ] }
+            ch_fusion_list_filtered = ch_fusion_list
+            ch_fusionreport_csv     = null
+            ch_fusionreport_report  = null
         } else {
-            error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with --arriba, --starfusion and/or --fusioncatcher")
+            error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with --arriba, --starfusion and/or --fusioncatcher with --skip_vis disabled")
         }
 
         //
@@ -252,46 +255,52 @@ workflow RNAFUSION {
     //
     // MODULE: MultiQC
     //
-    ch_multiqc_config        = Channel.fromPath(
-        "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
-    ch_multiqc_custom_config = params.multiqc_config ?
-        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
-        Channel.empty()
-    ch_multiqc_logo          = params.multiqc_logo ?
-        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
-        Channel.empty()
-
-    summary_params      = paramsSummaryMap(
-        workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
-    ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
-        file(params.multiqc_methods_description, checkIfExists: true) :
-        file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = Channel.value(
-        methodsDescriptionText(ch_multiqc_custom_methods_description))
-
-    ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
-    ch_multiqc_files = ch_multiqc_files.mix(
-        ch_methods_description.collectFile(
-            name: 'methods_description_mqc.yaml',
-            sort: true
+
+    def ch_multiqc_output = Channel.empty()
+    if(!params.skip_qc) {
+        ch_multiqc_config        = Channel.fromPath(
+            "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
+        ch_multiqc_custom_config = params.multiqc_config ?
+            Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+            Channel.empty()
+        ch_multiqc_logo          = params.multiqc_logo ?
+            Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+            Channel.empty()
+
+        summary_params      = paramsSummaryMap(
+            workflow, parameters_schema: "nextflow_schema.json")
+        ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+        ch_multiqc_files = ch_multiqc_files.mix(
+            ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
+        ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
+            file(params.multiqc_methods_description, checkIfExists: true) :
+            file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
+        ch_methods_description                = Channel.value(
+            methodsDescriptionText(ch_multiqc_custom_methods_description))
+
+        ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)
+        ch_multiqc_files = ch_multiqc_files.mix(
+            ch_methods_description.collectFile(
+                name: 'methods_description_mqc.yaml',
+                sort: true
+            )
         )
-    )
 
-    MULTIQC (
-        ch_multiqc_files.collect(),
-        ch_multiqc_config.toList(),
-        ch_multiqc_custom_config.toList(),
-        ch_multiqc_logo.toList(),
-        [],
-        []
-    )
+        MULTIQC (
+            ch_multiqc_files.collect(),
+            ch_multiqc_config.toList(),
+            ch_multiqc_custom_config.toList(),
+            ch_multiqc_logo.toList(),
+            [],
+            []
+        )
+        ch_multiqc_output = MULTIQC.out.report.toList()
+    }
+
 
     emit:
-    multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html
-    versions       = ch_versions                 // channel: [ path(versions.yml) ]
+    multiqc_report =  ch_multiqc_output // channel: /path/to/multiqc_report.html
+    versions       = ch_versions        // channel: [ path(versions.yml) ]
 
 }
 

From ad1558733bbde379978426a50a763551237ca68b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 31 Mar 2025 17:53:55 +0200
Subject: [PATCH 374/887] fix subwf input issue

---
 workflows/rnafusion.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index b8fa3874f..fdaf4ce75 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -217,7 +217,8 @@ workflow RNAFUSION {
             BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
             BUILD_REFERENCES.out.ch_hgnc_ref,
             BUILD_REFERENCES.out.ch_hgnc_date,
-            params.skip_vis
+            params.skip_vis,
+            params.skip_vcf
         )
         ch_versions      = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
         ch_multiqc_files = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))

From ea2d6efc2dd263c28b669d9c5c04973d2191a7bb Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 10:25:48 +0200
Subject: [PATCH 375/887] remove unused files

---
 assets/dummy_file_arriba.txt        | 0
 assets/dummy_file_fusioncatcher.txt | 0
 assets/dummy_file_pizzly.txt        | 0
 assets/dummy_file_squid.txt         | 0
 assets/dummy_file_starfusion.txt    | 0
 5 files changed, 0 insertions(+), 0 deletions(-)
 delete mode 100644 assets/dummy_file_arriba.txt
 delete mode 100644 assets/dummy_file_fusioncatcher.txt
 delete mode 100644 assets/dummy_file_pizzly.txt
 delete mode 100644 assets/dummy_file_squid.txt
 delete mode 100644 assets/dummy_file_starfusion.txt

diff --git a/assets/dummy_file_arriba.txt b/assets/dummy_file_arriba.txt
deleted file mode 100644
index e69de29bb..000000000
diff --git a/assets/dummy_file_fusioncatcher.txt b/assets/dummy_file_fusioncatcher.txt
deleted file mode 100644
index e69de29bb..000000000
diff --git a/assets/dummy_file_pizzly.txt b/assets/dummy_file_pizzly.txt
deleted file mode 100644
index e69de29bb..000000000
diff --git a/assets/dummy_file_squid.txt b/assets/dummy_file_squid.txt
deleted file mode 100644
index e69de29bb..000000000
diff --git a/assets/dummy_file_starfusion.txt b/assets/dummy_file_starfusion.txt
deleted file mode 100644
index e69de29bb..000000000

From 86828281228feafaf8024c2f94d6cc6cce5b528b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 10:25:56 +0200
Subject: [PATCH 376/887] fix param name

---
 main.nf         | 2 +-
 nextflow.config | 1 +
 2 files changed, 2 insertions(+), 1 deletion(-)

diff --git a/main.nf b/main.nf
index 2c3045f45..6280ac6dd 100644
--- a/main.nf
+++ b/main.nf
@@ -47,7 +47,7 @@ workflow {
         "starindex",
         "starfusion",
         "stringtie",
-        "fusion_report",
+        "fusionreport",
         "fastp",
         "salmon"
     ]
diff --git a/nextflow.config b/nextflow.config
index 5d45390f1..bdb6cc8e4 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -61,6 +61,7 @@ params {
     stringtie                  = false
     fusionreport               = false
     fastp                      = false
+    salmon                     = false
 
     // Skip steps
     skip_qc                    = false

From 2e4faec52ef093e4bcd98e4a600be56fe03e5638 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 10:26:03 +0200
Subject: [PATCH 377/887] fix build references

---
 subworkflows/local/build_references.nf | 163 ++++++++++++++-----------
 workflows/rnafusion.nf                 |  56 +++++----
 2 files changed, 122 insertions(+), 97 deletions(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 5d3be6277..cd2d73f76 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -38,8 +38,10 @@ workflow BUILD_REFERENCES {
     tools // list of all the tools to create references from
 
     main:
-    ch_versions = Channel.empty()
+    def ch_versions = Channel.empty()
 
+    def ch_fasta = Channel.empty()
+    def ch_gtf   = Channel.empty()
     if (!exists_not_empty(params.fasta) || !exists_not_empty(params.gtf)){
         GENCODE_DOWNLOAD(params.genome_gencode_version, params.genome)
         ch_versions = ch_versions.mix(GENCODE_DOWNLOAD.out.versions)
@@ -50,6 +52,7 @@ workflow BUILD_REFERENCES {
         ch_gtf = Channel.fromPath(params.gtf).map { that -> [[id:that.Name], that] }
     }
 
+    def ch_fai = Channel.empty()
     if (!exists_not_empty(params.fai)){
         SAMTOOLS_FAIDX(ch_fasta, [[],[]])
         ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions)
@@ -58,6 +61,8 @@ workflow BUILD_REFERENCES {
         ch_fai = Channel.fromPath(params.fai).map { that -> [[id:that.Name], that] }
     }
 
+    def ch_hgnc_date = Channel.empty()
+    def ch_hgnc_ref  = Channel.empty()
     if ((!exists_not_empty(params.hgnc_ref) || !exists_not_empty(params.hgnc_date)) && !params.skip_vcf){
         HGNC_DOWNLOAD( )
         ch_versions = ch_versions.mix(HGNC_DOWNLOAD.out.versions)
@@ -68,6 +73,7 @@ workflow BUILD_REFERENCES {
         ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
     }
 
+    def ch_rrna_interval = Channel.empty()
     if (!exists_not_empty(params.rrna_intervals)){
         GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
         ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
@@ -80,6 +86,7 @@ workflow BUILD_REFERENCES {
         ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
     }
 
+    def ch_refflat = Channel.empty()
     if (!exists_not_empty(params.refflat)){
         GTF_TO_REFFLAT(ch_gtf)
         ch_versions = ch_versions.mix(GTF_TO_REFFLAT.out.versions)
@@ -89,56 +96,67 @@ workflow BUILD_REFERENCES {
     }
 
     def run_salmon = tools.contains("salmon")
-    if (run_salmon && (!exists_not_empty(params.salmon_index) || !exists_not_empty(params.salmon_index_stub_check))){ // add condition for qc
-        GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
-        ch_versions = ch_versions.mix(GFFREAD.out.versions)
-        SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
-        ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
-        ch_salmon_index = SALMON_INDEX.out.index
-    } else {
-        ch_salmon_index = Channel.fromPath({params.salmon_index})
+    def ch_salmon_index = Channel.empty()
+    if (run_salmon) {
+        if (!exists_not_empty(params.salmon_index) || !exists_not_empty(params.salmon_index_stub_check)){ // add condition for qc
+            GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
+            ch_versions = ch_versions.mix(GFFREAD.out.versions)
+            SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
+            ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
+            ch_salmon_index = SALMON_INDEX.out.index
+        } else {
+            ch_salmon_index = Channel.fromPath({params.salmon_index})
+        }
     }
 
+    def ch_starindex_ref = Channel.empty()
     def star_index_tools = tools.intersect(["starindex", "starfusion", "arriba", "ctatsplicing"])
-    if (star_index_tools.size() > 0 && (!exists_not_empty(params.starindex_ref) || !exists_not_empty(params.starindex_ref_stub_check))) {
-        STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
-        ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions)
-        ch_starindex_ref = STAR_GENOMEGENERATE.out.index
-    } else {
-        ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
+    if (star_index_tools) {
+        if (!exists_not_empty(params.starindex_ref) || !exists_not_empty(params.starindex_ref_stub_check)) {
+            STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
+            ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions)
+            ch_starindex_ref = STAR_GENOMEGENERATE.out.index
+        } else {
+            ch_starindex_ref = Channel.fromPath(params.starindex_ref).map { that -> [[id:that.Name], that] }
+        }
     }
 
-    if (tools.contains("arriba") && (
-            !exists_not_empty(params.arriba_ref_blacklist) ||
-            !exists_not_empty(params.arriba_ref_known_fusions) ||
-            !exists_not_empty(params.arriba_ref_protein_domains)
-        )
-    ) {
-        ARRIBA_DOWNLOAD(params.genome)
-        ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)
-        ch_arriba_ref_blacklist = ARRIBA_DOWNLOAD.out.blacklist
-        ch_arriba_ref_cytobands = ARRIBA_DOWNLOAD.out.cytobands
-        ch_arriba_ref_known_fusions = ARRIBA_DOWNLOAD.out.known_fusions
-        ch_arriba_ref_protein_domains = ARRIBA_DOWNLOAD.out.protein_domains
-    } else {
-        ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist)
-        ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands)
-        ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions)
-        ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
+    def ch_arriba_ref_blacklist       = Channel.empty()
+    def ch_arriba_ref_cytobands       = Channel.empty()
+    def ch_arriba_ref_known_fusions   = Channel.empty()
+    def ch_arriba_ref_protein_domains = Channel.empty()
+    if (tools.contains("arriba")) {
+        if (!exists_not_empty(params.arriba_ref_blacklist) || !exists_not_empty(params.arriba_ref_known_fusions) || !exists_not_empty(params.arriba_ref_protein_domains)) {
+            ARRIBA_DOWNLOAD(params.genome)
+            ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)
+            ch_arriba_ref_blacklist       = ARRIBA_DOWNLOAD.out.blacklist
+            ch_arriba_ref_cytobands       = ARRIBA_DOWNLOAD.out.cytobands
+            ch_arriba_ref_known_fusions   = ARRIBA_DOWNLOAD.out.known_fusions
+            ch_arriba_ref_protein_domains = ARRIBA_DOWNLOAD.out.protein_domains
+        } else {
+            ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist)
+            ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands)
+            ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions)
+            ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
+        }
     }
 
-
-    if (tools.contains("fusioncatcher") && (!exists_not_empty(params.fusioncatcher_ref) || !exists_not_empty(params.fusioncatcher_ref_stub_check))) {
-            FUSIONCATCHER_BUILD(params.genome_gencode_version)
-            ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
-            ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
-    }
-    else {
-        ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref)
+    def ch_fusioncatcher_ref = Channel.empty()
+    if (tools.contains("fusioncatcher")) {
+        if (!exists_not_empty(params.fusioncatcher_ref) || !exists_not_empty(params.fusioncatcher_ref_stub_check)) {
+                FUSIONCATCHER_BUILD(params.genome_gencode_version)
+                ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
+                ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
+        }
+        else {
+            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref)
+        }
     }
 
+    def ch_starfusion_ref = Channel.empty()
     def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing"])
-    if (starfusion_tools.size() > 0 && (!exists_not_empty(params.starfusion_ref) || !exists_not_empty(params.starfusion_ref_stub_check))) {
+    if (starfusion_tools) {
+        if (!exists_not_empty(params.starfusion_ref) || !exists_not_empty(params.starfusion_ref_stub_check)) {
             STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
@@ -151,41 +169,44 @@ workflow BUILD_REFERENCES {
             } else {
                 ch_starfusion_ref = STARFUSION_BUILD.out.reference
             }
+        }
+        else {
+            ch_starfusion_ref = Channel.fromPath(params.starfusion_ref)
+        }
     }
-    else {
-        ch_starfusion_ref = Channel.fromPath(params.starfusion_ref)
-    }
-
 
-    if (tools.contains("fusionreport") && (!exists_not_empty(params.fusionreport_ref) || !exists_not_empty(params.fusionreport_ref_stub_check))) {
-        if (!params.no_cosmic && (!params.cosmic_username || !params.cosmic_passwd)) {
-            error('COSMIC username and/or password missing, this is needed to download the fusionreport reference')
+    def ch_fusionreport_ref = Channel.empty()
+    if (tools.contains("fusionreport")) {
+        if (!exists_not_empty(params.fusionreport_ref) || !exists_not_empty(params.fusionreport_ref_stub_check)) {
+            if (!params.no_cosmic && (!params.cosmic_username || !params.cosmic_passwd)) {
+                error('COSMIC username and/or password missing, this is needed to download the fusionreport reference')
+            }
+            FUSIONREPORT_DOWNLOAD()
+            ch_versions = ch_versions.mix(FUSIONREPORT_DOWNLOAD.out.versions)
+            ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
+        } else {
+            ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
         }
-        FUSIONREPORT_DOWNLOAD()
-        ch_versions = ch_versions.mix(FUSIONREPORT_DOWNLOAD.out.versions)
-        ch_fusionreport_ref = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
-    } else {
-        ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { that -> [[id:that.Name], that] }
     }
 
     emit:
-    ch_fasta
-    ch_gtf
-    ch_fai
-    ch_hgnc_ref
-    ch_hgnc_date
-    ch_rrna_interval
-    ch_refflat
-    ch_salmon_index
-    ch_starindex_ref
-    ch_arriba_ref_blacklist
-    ch_arriba_ref_cytobands
-    ch_arriba_ref_known_fusions
-    ch_arriba_ref_protein_domains
-    ch_fusioncatcher_ref
-    ch_starfusion_ref
-    ch_fusionreport_ref
-    versions        = ch_versions
+    fasta                       = ch_fasta
+    gtf                         = ch_gtf
+    fai                         = ch_fai
+    hgnc_ref                    = ch_hgnc_ref
+    hgnc_date                   = ch_hgnc_date
+    rrna_interval               = ch_rrna_interval
+    refflat                     = ch_refflat
+    salmon_index                = ch_salmon_index
+    starindex_ref               = ch_starindex_ref
+    arriba_ref_blacklist        = ch_arriba_ref_blacklist
+    arriba_ref_cytobands        = ch_arriba_ref_cytobands
+    arriba_ref_known_fusions    = ch_arriba_ref_known_fusions
+    arriba_ref_protein_domains  = ch_arriba_ref_protein_domains
+    fusioncatcher_ref           = ch_fusioncatcher_ref
+    starfusion_ref              = ch_starfusion_ref
+    fusionreport_ref            = ch_fusionreport_ref
+    versions                    = ch_versions
 }
 
 /*
@@ -195,6 +216,6 @@ workflow BUILD_REFERENCES {
 */
 
 def exists_not_empty(path) {
-    def path_to_check = file(path)
+    def path_to_check = file(path as String)
     return path_to_check.exists() && !path_to_check.isEmpty()
 }
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index fdaf4ce75..37d484c61 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -87,7 +87,7 @@ workflow RNAFUSION {
         }
 
         if(tools.contains("salmon")) {
-            SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.ch_salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.ch_gtf.map{ it -> it[1] }, [], false, 'A')
+            SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.gtf.map{ it -> it[1] }, [], false, 'A')
             ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
             ch_versions      = ch_versions.mix(SALMON_QUANT.out.versions)
         }
@@ -100,18 +100,20 @@ workflow RNAFUSION {
         // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
         // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
 
+        def fusions_created = false
         def ch_arriba_fusions = ch_reads.map { it -> [it[0], []] } // Set arriba fusions to empty by default
         if(tools.intersect(["arriba", "ctatsplicing"])) {
+            fusions_created = true
             ARRIBA_WORKFLOW (
                 ch_reads,
-                BUILD_REFERENCES.out.ch_gtf,
-                BUILD_REFERENCES.out.ch_fasta,
-                BUILD_REFERENCES.out.ch_starindex_ref,
-                BUILD_REFERENCES.out.ch_arriba_ref_blacklist,
-                BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
-                BUILD_REFERENCES.out.ch_arriba_ref_known_fusions,
-                BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
-                BUILD_REFERENCES.out.ch_starfusion_ref,
+                BUILD_REFERENCES.out.gtf,
+                BUILD_REFERENCES.out.fasta,
+                BUILD_REFERENCES.out.starindex_ref,
+                BUILD_REFERENCES.out.arriba_ref_blacklist,
+                BUILD_REFERENCES.out.arriba_ref_cytobands,
+                BUILD_REFERENCES.out.arriba_ref_known_fusions,
+                BUILD_REFERENCES.out.arriba_ref_protein_domains,
+                BUILD_REFERENCES.out.starfusion_ref,
                 tools,
                 params.star_ignore_sjdbgtf,      // boolean
                 params.seq_center ?: '',         // string
@@ -129,12 +131,13 @@ workflow RNAFUSION {
         def ch_starfusion_fusions = ch_reads.map { it -> [it[0], []] } // Set starfusion fusions to empty by default
         def ch_starfusion_bams = Channel.empty()
         if(tools.intersect(["starfusion", "ctatsplicing", "stringtie"])) {
+            fusions_created = true
             STARFUSION_WORKFLOW (
                 ch_reads,
-                BUILD_REFERENCES.out.ch_gtf,
-                BUILD_REFERENCES.out.ch_starindex_ref,
-                BUILD_REFERENCES.out.ch_fasta,
-                BUILD_REFERENCES.out.ch_starfusion_ref,
+                BUILD_REFERENCES.out.gtf,
+                BUILD_REFERENCES.out.starindex_ref,
+                BUILD_REFERENCES.out.fasta,
+                BUILD_REFERENCES.out.starfusion_ref,
                 tools
             )
             ch_versions             = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
@@ -150,9 +153,10 @@ workflow RNAFUSION {
 
         def ch_fusioncatcher_fusions = ch_reads.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
+            fusions_created = true
             FUSIONCATCHER_WORKFLOW (
                 ch_reads,
-                BUILD_REFERENCES.out.ch_fusioncatcher_ref,       // channel [ meta, path       ]
+                BUILD_REFERENCES.out.fusioncatcher_ref,       // channel [ meta, path       ]
                 params.fusioncatcher_fusions
             )
             ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
@@ -165,7 +169,7 @@ workflow RNAFUSION {
         if(tools.contains("stringtie")) {
             STRINGTIE_WORKFLOW (
                 STARFUSION_WORKFLOW.out.ch_bam_sorted,
-                BUILD_REFERENCES.out.ch_gtf
+                BUILD_REFERENCES.out.gtf
             )
             ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
         }
@@ -178,10 +182,10 @@ workflow RNAFUSION {
         def ch_fusion_list_filtered = Channel.empty()
         def ch_fusionreport_report = Channel.empty()
         def ch_fusionreport_csv = Channel.empty()
-        if(tools.intersect(["arriba", "starfusion", "fusioncatcher"]) && !params.skip_vis) {
+        if(fusions_created && !params.skip_vis) {
             FUSIONREPORT_WORKFLOW (
                 ch_reads,
-                BUILD_REFERENCES.out.ch_fusionreport_ref,
+                BUILD_REFERENCES.out.fusionreport_ref,
                 ch_arriba_fusions,
                 ch_starfusion_fusions,
                 ch_fusioncatcher_fusions
@@ -212,11 +216,11 @@ workflow RNAFUSION {
             ch_fusionreport_report,
             ch_fusionreport_csv,
             ch_starfusion_bams,
-            BUILD_REFERENCES.out.ch_gtf,
-            BUILD_REFERENCES.out.ch_arriba_ref_protein_domains,
-            BUILD_REFERENCES.out.ch_arriba_ref_cytobands,
-            BUILD_REFERENCES.out.ch_hgnc_ref,
-            BUILD_REFERENCES.out.ch_hgnc_date,
+            BUILD_REFERENCES.out.gtf,
+            BUILD_REFERENCES.out.arriba_ref_protein_domains,
+            BUILD_REFERENCES.out.arriba_ref_cytobands,
+            BUILD_REFERENCES.out.hgnc_ref,
+            BUILD_REFERENCES.out.hgnc_date,
             params.skip_vis,
             params.skip_vcf
         )
@@ -230,10 +234,10 @@ workflow RNAFUSION {
         if(!params.skip_qc) {
             QC_WORKFLOW (
                 STARFUSION_WORKFLOW.out.ch_bam_sorted,
-                BUILD_REFERENCES.out.ch_refflat,
-                BUILD_REFERENCES.out.ch_fasta,
-                BUILD_REFERENCES.out.ch_fai,
-                BUILD_REFERENCES.out.ch_rrna_interval
+                BUILD_REFERENCES.out.refflat,
+                BUILD_REFERENCES.out.fasta,
+                BUILD_REFERENCES.out.fai,
+                BUILD_REFERENCES.out.rrna_interval
             )
             ch_versions      = ch_versions.mix(QC_WORKFLOW.out.versions)
             ch_multiqc_files = ch_multiqc_files.mix(QC_WORKFLOW.out.rnaseq_metrics.collect{it[1]})

From 3898f921e2354c7828031933be99bfa4995a26d0 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 11:33:52 +0200
Subject: [PATCH 378/887] fix some flow issues

---
 main.nf                                       |  3 +-
 modules/local/fusioninspector/main.nf         |  2 +-
 modules/local/fusionreport/detect/main.nf     |  6 +-
 nextflow.config                               |  1 +
 nextflow_schema.json                          |  5 +
 subworkflows/local/build_references.nf        | 97 ++++++++++---------
 .../local/ctatsplicing_workflow/main.nf       | 25 +++--
 .../local/fusioninspector_workflow.nf         | 21 ++--
 subworkflows/local/qc_workflow/main.nf        | 17 +++-
 .../local/starfusion_workflow/main.nf         |  2 +-
 subworkflows/local/stringtie_workflow/main.nf | 30 +++---
 workflows/rnafusion.nf                        | 59 ++++++-----
 12 files changed, 149 insertions(+), 119 deletions(-)

diff --git a/main.nf b/main.nf
index 6280ac6dd..35d5817a7 100644
--- a/main.nf
+++ b/main.nf
@@ -49,7 +49,8 @@ workflow {
         "stringtie",
         "fusionreport",
         "fastp",
-        "salmon"
+        "salmon",
+        "fusioninspector"
     ]
 
     def tools = params.all ? all_tools : all_tools.findAll { tool ->
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 3659220b0..ae368b5e7 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -9,7 +9,7 @@ process FUSIONINSPECTOR {
 
     input:
     tuple val(meta), path(reads), path(fusion_list)
-    path reference
+    tuple val(meta2), path(reference)
 
     output:
     tuple val(meta), path("*FusionInspector.fusions.tsv")                  , emit: tsv
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index 3670eec0b..dab70ffe4 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -28,9 +28,9 @@ process FUSIONREPORT {
     script:
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
-    def tools = params.arriba || params.all         ? "--arriba ${arriba_fusions} " : ''
-    tools    += params.starfusion  || params.all    ? "--starfusion ${starfusion_fusions} " : ''
-    tools    += params.fusioncatcher  || params.all ? "--fusioncatcher ${fusioncatcher_fusions} " : ''
+    def tools = arriba_fusions        ? "--arriba ${arriba_fusions} " : ''
+    tools    += starfusion_fusions    ? "--starfusion ${starfusion_fusions} " : ''
+    tools    += fusioncatcher_fusions ? "--fusioncatcher ${fusioncatcher_fusions} " : ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     fusion_report run $meta.id . $fusionreport_ref $tools --allow-multiple-gene-symbols --tool-cutoff $tools_cutoff $args $args2
diff --git a/nextflow.config b/nextflow.config
index bdb6cc8e4..b82faf984 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -60,6 +60,7 @@ params {
     starfusion                 = false
     stringtie                  = false
     fusionreport               = false
+    fusioninspector            = false
     fastp                      = false
     salmon                     = false
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f64cf478c..acd1758c9 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -186,6 +186,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to file in fusioncatcher references"
                 },
+                "fusioninspector": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Run fusioninspector"
+                },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index cd2d73f76..228dc58e0 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -63,41 +63,47 @@ workflow BUILD_REFERENCES {
 
     def ch_hgnc_date = Channel.empty()
     def ch_hgnc_ref  = Channel.empty()
-    if ((!exists_not_empty(params.hgnc_ref) || !exists_not_empty(params.hgnc_date)) && !params.skip_vcf){
-        HGNC_DOWNLOAD( )
-        ch_versions = ch_versions.mix(HGNC_DOWNLOAD.out.versions)
-        ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref
-        ch_hgnc_date = HGNC_DOWNLOAD.out.hgnc_date
-    } else {
-        ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { that -> [[id:that.Name], that] }
-        ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
+    def run_fusioninspector = tools.contains("fusioninspector")
+    if(run_fusioninspector) {
+        if ((!exists_not_empty(params.hgnc_ref) || !exists_not_empty(params.hgnc_date)) && !params.skip_vcf){
+            HGNC_DOWNLOAD( )
+            ch_versions = ch_versions.mix(HGNC_DOWNLOAD.out.versions)
+            ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref
+            ch_hgnc_date = HGNC_DOWNLOAD.out.hgnc_date
+        } else {
+            ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { that -> [[id:that.Name], that] }
+            ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }
+        }
     }
 
     def ch_rrna_interval = Channel.empty()
-    if (!exists_not_empty(params.rrna_intervals)){
-        GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
-        ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
-        GET_RRNA_TRANSCRIPTS(ch_gtf)
-        ch_versions = ch_versions.mix(GET_RRNA_TRANSCRIPTS.out.versions)
-        GATK4_BEDTOINTERVALLIST(GET_RRNA_TRANSCRIPTS.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
-        ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions)
-        ch_rrna_interval = GATK4_BEDTOINTERVALLIST.out.interval_list
-    } else {
-        ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
+    if (!params.skip_qc) {
+        if (!exists_not_empty(params.rrna_intervals)){
+            GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
+            ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
+            GET_RRNA_TRANSCRIPTS(ch_gtf)
+            ch_versions = ch_versions.mix(GET_RRNA_TRANSCRIPTS.out.versions)
+            GATK4_BEDTOINTERVALLIST(GET_RRNA_TRANSCRIPTS.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
+            ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions)
+            ch_rrna_interval = GATK4_BEDTOINTERVALLIST.out.interval_list
+        } else {
+            ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }
+        }
     }
 
     def ch_refflat = Channel.empty()
-    if (!exists_not_empty(params.refflat)){
-        GTF_TO_REFFLAT(ch_gtf)
-        ch_versions = ch_versions.mix(GTF_TO_REFFLAT.out.versions)
-        ch_refflat = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
-    } else {
-        ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
+    if (!params.skip_qc) {
+        if (!exists_not_empty(params.refflat)){
+            GTF_TO_REFFLAT(ch_gtf)
+            ch_versions = ch_versions.mix(GTF_TO_REFFLAT.out.versions)
+            ch_refflat = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
+        } else {
+            ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
+        }
     }
 
-    def run_salmon = tools.contains("salmon")
     def ch_salmon_index = Channel.empty()
-    if (run_salmon) {
+    if (tools.contains("salmon")) {
         if (!exists_not_empty(params.salmon_index) || !exists_not_empty(params.salmon_index_stub_check)){ // add condition for qc
             GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
             ch_versions = ch_versions.mix(GFFREAD.out.versions)
@@ -110,7 +116,7 @@ workflow BUILD_REFERENCES {
     }
 
     def ch_starindex_ref = Channel.empty()
-    def star_index_tools = tools.intersect(["starindex", "starfusion", "arriba", "ctatsplicing"])
+    def star_index_tools = tools.intersect(["starindex", "starfusion", "arriba", "ctatsplicing", "stringtie"])
     if (star_index_tools) {
         if (!exists_not_empty(params.starindex_ref) || !exists_not_empty(params.starindex_ref_stub_check)) {
             STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
@@ -125,7 +131,8 @@ workflow BUILD_REFERENCES {
     def ch_arriba_ref_cytobands       = Channel.empty()
     def ch_arriba_ref_known_fusions   = Channel.empty()
     def ch_arriba_ref_protein_domains = Channel.empty()
-    if (tools.contains("arriba")) {
+    def arriba_tools = tools.intersect(["arriba", "fusioninspector"])
+    if (arriba_tools) {
         if (!exists_not_empty(params.arriba_ref_blacklist) || !exists_not_empty(params.arriba_ref_known_fusions) || !exists_not_empty(params.arriba_ref_protein_domains)) {
             ARRIBA_DOWNLOAD(params.genome)
             ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)
@@ -154,7 +161,7 @@ workflow BUILD_REFERENCES {
     }
 
     def ch_starfusion_ref = Channel.empty()
-    def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing"])
+    def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing", "fusioninspector", "stringtie"])
     if (starfusion_tools) {
         if (!exists_not_empty(params.starfusion_ref) || !exists_not_empty(params.starfusion_ref_stub_check)) {
             STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
@@ -190,22 +197,22 @@ workflow BUILD_REFERENCES {
     }
 
     emit:
-    fasta                       = ch_fasta
-    gtf                         = ch_gtf
-    fai                         = ch_fai
-    hgnc_ref                    = ch_hgnc_ref
-    hgnc_date                   = ch_hgnc_date
-    rrna_interval               = ch_rrna_interval
-    refflat                     = ch_refflat
-    salmon_index                = ch_salmon_index
-    starindex_ref               = ch_starindex_ref
-    arriba_ref_blacklist        = ch_arriba_ref_blacklist
-    arriba_ref_cytobands        = ch_arriba_ref_cytobands
-    arriba_ref_known_fusions    = ch_arriba_ref_known_fusions
-    arriba_ref_protein_domains  = ch_arriba_ref_protein_domains
-    fusioncatcher_ref           = ch_fusioncatcher_ref
-    starfusion_ref              = ch_starfusion_ref
-    fusionreport_ref            = ch_fusionreport_ref
+    fasta                       = ch_fasta.collect()
+    gtf                         = ch_gtf.collect()
+    fai                         = ch_fai.collect()
+    hgnc_ref                    = ch_hgnc_ref.collect()
+    hgnc_date                   = ch_hgnc_date.collect()
+    rrna_interval               = ch_rrna_interval.collect()
+    refflat                     = ch_refflat.collect()
+    salmon_index                = ch_salmon_index.collect()
+    starindex_ref               = ch_starindex_ref.collect()
+    arriba_ref_blacklist        = ch_arriba_ref_blacklist.collect()
+    arriba_ref_cytobands        = ch_arriba_ref_cytobands.collect()
+    arriba_ref_known_fusions    = ch_arriba_ref_known_fusions.collect()
+    arriba_ref_protein_domains  = ch_arriba_ref_protein_domains.collect()
+    fusioncatcher_ref           = ch_fusioncatcher_ref.collect()
+    starfusion_ref              = ch_starfusion_ref.collect()
+    fusionreport_ref            = ch_fusionreport_ref.collect()
     versions                    = ch_versions
 }
 
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
index 8b279033d..f8b2d3592 100644
--- a/subworkflows/local/ctatsplicing_workflow/main.nf
+++ b/subworkflows/local/ctatsplicing_workflow/main.nf
@@ -10,21 +10,18 @@ workflow CTATSPLICING_WORKFLOW {
     main:
     def ch_versions = Channel.empty()
 
-    if (params.ctatsplicing || params.all) {
-        def ch_ctatsplicing_input = split_junctions
-            .join(junctions, failOnMismatch:true, failOnDuplicate:true)
-            .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
-            .map { meta, split_junction, junction, bam ->
-                [ meta, split_junction, junction, bam, [] ]
-            }
+    def ch_ctatsplicing_input = split_junctions
+        .join(junctions, failOnMismatch:true, failOnDuplicate:true)
+        .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
+        .map { meta, split_junction, junction, bam ->
+            [ meta, split_junction, junction, bam, [] ]
+        }
 
-        CTATSPLICING_STARTOCANCERINTRONS(
-            ch_ctatsplicing_input,
-            ctat_genome_lib
-        )
-        ch_versions = ch_versions.mix(CTATSPLICING_STARTOCANCERINTRONS.out.versions.first())
-
-    }
+    CTATSPLICING_STARTOCANCERINTRONS(
+        ch_ctatsplicing_input,
+        ctat_genome_lib
+    )
+    ch_versions = ch_versions.mix(CTATSPLICING_STARTOCANCERINTRONS.out.versions.first())
 
     emit:
     versions              = ch_versions
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index e17b58974..56fd67321 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -17,22 +17,18 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         ch_arriba_ref_cytobands
         ch_hgnc_ref
         ch_hgnc_date
+        ch_starfusion_ref
         skip_vis
         skip_vcf
 
     main:
         ch_versions = Channel.empty()
         ch_arriba_visualisation = Channel.empty()
-        index ="${params.starfusion_ref}"
 
         ch_fusion_list = ( params.tools_cutoff > 1 ? fusion_list_filtered : fusion_list )
-        .branch{
-            no_fusions: it[1].size() == 0
-            fusions: it[1].size() > 0
-        }
 
         if (params.whitelist)  {
-            ch_whitelist = ch_fusion_list.fusions.combine(Channel.value(file(params.whitelist, checkIfExists:true)))
+            ch_whitelist = ch_fusion_list.combine(Channel.value(file(params.whitelist, checkIfExists:true)))
                             .map { meta, fusions, whitelist -> [ meta, [fusions, whitelist] ] }
 
             CAT_CAT(ch_whitelist) // fusioninspector takes care of possible duplicates
@@ -40,17 +36,18 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             ch_reads_fusion = reads.join(CAT_CAT.out.file_out )
         }
         else {
-            ch_reads_fusion = reads.join(ch_fusion_list.fusions )
+            ch_reads_fusion = reads.join(ch_fusion_list)
         }
 
-        FUSIONINSPECTOR( ch_reads_fusion, index)
+        FUSIONINSPECTOR( ch_reads_fusion, ch_starfusion_ref)
         ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
 
-        AGAT_CONVERTSPGFF2TSV(FUSIONINSPECTOR.out.out_gtf)
-        ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
-
-        fusion_data = FUSIONINSPECTOR.out.tsv_coding_effect.join(AGAT_CONVERTSPGFF2TSV.out.tsv).join(fusionreport_out).join(fusionreport_csv)
         if(!skip_vcf) {
+            AGAT_CONVERTSPGFF2TSV(FUSIONINSPECTOR.out.out_gtf)
+            ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
+
+            fusion_data = FUSIONINSPECTOR.out.tsv_coding_effect.join(AGAT_CONVERTSPGFF2TSV.out.tsv).join(fusionreport_out).join(fusionreport_csv)
+
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)
         }
diff --git a/subworkflows/local/qc_workflow/main.nf b/subworkflows/local/qc_workflow/main.nf
index 4b6352420..4924b633b 100644
--- a/subworkflows/local/qc_workflow/main.nf
+++ b/subworkflows/local/qc_workflow/main.nf
@@ -17,15 +17,26 @@ workflow QC_WORKFLOW {
     main:
         ch_versions = Channel.empty()
 
-        PICARD_COLLECTRNASEQMETRICS(ch_bam_sorted, ch_refflat.map{ meta, refflat -> [ refflat ] }, ch_fasta.map{ meta, fasta -> [ fasta ] }, ch_rrna_interval.map{ meta, intervals -> [ intervals ] }.ifEmpty([]) ) // Some chromosome or annotation may not have rRNA genes
+        PICARD_COLLECTRNASEQMETRICS(
+            ch_bam_sorted.map { meta, bam, _bai -> [ meta, bam ]},
+            ch_refflat.map{ _meta, refflat -> [ refflat ] },
+            ch_fasta.map{ _meta, fasta -> [ fasta ] },
+            ch_rrna_interval.map{ _meta, intervals -> [ intervals ] }.ifEmpty([])
+        ) // Some chromosome or annotation may not have rRNA genes
         ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
         ch_rnaseq_metrics = PICARD_COLLECTRNASEQMETRICS.out.metrics
 
-        GATK4_MARKDUPLICATES(ch_bam_sorted, ch_fasta.map { meta, fasta -> [ fasta ]}, ch_fai.map { meta, fasta_fai -> [ fasta_fai ]})
+        GATK4_MARKDUPLICATES(
+            ch_bam_sorted.map { meta, bam, _bai -> [ meta, bam ]},
+            ch_fasta.map { _meta, fasta -> [ fasta ]},
+            ch_fai.map { _meta, fasta_fai -> [ fasta_fai ]}
+        )
         ch_versions = ch_versions.mix(GATK4_MARKDUPLICATES.out.versions)
         ch_duplicate_metrics = GATK4_MARKDUPLICATES.out.metrics
 
-        PICARD_COLLECTINSERTSIZEMETRICS(ch_bam_sorted)
+        PICARD_COLLECTINSERTSIZEMETRICS(
+            ch_bam_sorted.map { meta, bam, _bai -> [ meta, bam ]}
+        )
         ch_versions = ch_versions.mix(PICARD_COLLECTINSERTSIZEMETRICS.out.versions)
         ch_insertsize_metrics = PICARD_COLLECTINSERTSIZEMETRICS.out.metrics
 
diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index d4bee449b..ba39fe33c 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -51,7 +51,7 @@ workflow STARFUSION_WORKFLOW {
                 SAMTOOLS_INDEX_FOR_STARFUSION_CRAM (SAMTOOLS_VIEW_FOR_STARFUSION.out.cram)
                 ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION_CRAM.out.versions)
             }
-            if (params.starfusion || params.all){
+            if (tools.contains("starfusion")){
                 STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
                 ch_versions = ch_versions.mix(STARFUSION.out.versions)
                 ch_starfusion_fusions = STARFUSION.out.fusions
diff --git a/subworkflows/local/stringtie_workflow/main.nf b/subworkflows/local/stringtie_workflow/main.nf
index 25ff26ee8..3b27688c7 100644
--- a/subworkflows/local/stringtie_workflow/main.nf
+++ b/subworkflows/local/stringtie_workflow/main.nf
@@ -11,22 +11,20 @@ workflow STRINGTIE_WORKFLOW {
         ch_versions = Channel.empty()
         ch_stringtie_gtf = Channel.empty()
 
-        if ((params.stringtie || params.all) && !params.fusioninspector_only) {
-            STRINGTIE_STRINGTIE(bam_sorted, ch_chrgtf.map { meta, gtf -> [ gtf ]})
-            ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-            STRINGTIE_STRINGTIE
-                .out
-                .transcript_gtf
-                .map { it -> it[1] }
-                .set { stringtie_gtf }
-            ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-
-            STRINGTIE_MERGE (stringtie_gtf, ch_chrgtf.map { meta, gtf -> [ gtf ]})
-            ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
-            ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
-        }
+        STRINGTIE_STRINGTIE(bam_sorted, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
+
+        STRINGTIE_STRINGTIE
+            .out
+            .transcript_gtf
+            .map { it -> it[1] }
+            .set { stringtie_gtf }
+        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
+
+
+        STRINGTIE_MERGE (stringtie_gtf, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+        ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
+        ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
 
     emit:
         stringtie_gtf = ch_stringtie_gtf.ifEmpty(null) // channel: [meta, gtf]
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 37d484c61..857de11e4 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -87,7 +87,14 @@ workflow RNAFUSION {
         }
 
         if(tools.contains("salmon")) {
-            SALMON_QUANT( ch_reads, BUILD_REFERENCES.out.salmon_index.map{ it -> it[1] }, BUILD_REFERENCES.out.gtf.map{ it -> it[1] }, [], false, 'A')
+            SALMON_QUANT(
+                ch_reads.view(),
+                BUILD_REFERENCES.out.salmon_index,
+                BUILD_REFERENCES.out.gtf.map{ it -> it[1] },
+                [],
+                false,
+                'A'
+            )
             ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
             ch_versions      = ch_versions.mix(SALMON_QUANT.out.versions)
         }
@@ -168,7 +175,7 @@ workflow RNAFUSION {
 
         if(tools.contains("stringtie")) {
             STRINGTIE_WORKFLOW (
-                STARFUSION_WORKFLOW.out.ch_bam_sorted,
+                ch_starfusion_bams,
                 BUILD_REFERENCES.out.gtf
             )
             ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
@@ -182,7 +189,10 @@ workflow RNAFUSION {
         def ch_fusion_list_filtered = Channel.empty()
         def ch_fusionreport_report = Channel.empty()
         def ch_fusionreport_csv = Channel.empty()
-        if(fusions_created && !params.skip_vis) {
+        if(!params.skip_vis && tools.contains("fusionreport")) {
+            if (!fusions_created) {
+                error("Could not find any fusion files. Please generate some with --arriba, --starfusion and/or --fusioncatcher")
+            }
             FUSIONREPORT_WORKFLOW (
                 ch_reads,
                 BUILD_REFERENCES.out.fusionreport_ref,
@@ -201,31 +211,34 @@ workflow RNAFUSION {
             ch_fusion_list_filtered = ch_fusion_list
             ch_fusionreport_csv     = null
             ch_fusionreport_report  = null
-        } else {
-            error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with --arriba, --starfusion and/or --fusioncatcher with --skip_vis disabled")
+        } else if(tools.contains("fusioninspector")) {
+            error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with --arriba, --starfusion and/or --fusioncatcher with --skip_vis disabled and --fusionreport enabled")
         }
 
         //
         // SUBWORKFLOW: Run FusionInspector
         //
 
-        FUSIONINSPECTOR_WORKFLOW (
-            ch_reads,
-            ch_fusion_list,
-            ch_fusion_list_filtered,
-            ch_fusionreport_report,
-            ch_fusionreport_csv,
-            ch_starfusion_bams,
-            BUILD_REFERENCES.out.gtf,
-            BUILD_REFERENCES.out.arriba_ref_protein_domains,
-            BUILD_REFERENCES.out.arriba_ref_cytobands,
-            BUILD_REFERENCES.out.hgnc_ref,
-            BUILD_REFERENCES.out.hgnc_date,
-            params.skip_vis,
-            params.skip_vcf
-        )
-        ch_versions      = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
-        ch_multiqc_files = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
+        if (tools.contains("fusioninspector")) {
+            FUSIONINSPECTOR_WORKFLOW (
+                ch_reads,
+                ch_fusion_list,
+                ch_fusion_list_filtered,
+                ch_fusionreport_report,
+                ch_fusionreport_csv,
+                ch_starfusion_bams,
+                BUILD_REFERENCES.out.gtf,
+                BUILD_REFERENCES.out.arriba_ref_protein_domains,
+                BUILD_REFERENCES.out.arriba_ref_cytobands,
+                BUILD_REFERENCES.out.hgnc_ref,
+                BUILD_REFERENCES.out.hgnc_date,
+                BUILD_REFERENCES.out.starfusion_ref,
+                params.skip_vis,
+                params.skip_vcf
+            )
+            ch_versions      = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
+            ch_multiqc_files = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))
+        }
 
         //
         // SUBWORKFLOW: Run QC
@@ -233,7 +246,7 @@ workflow RNAFUSION {
 
         if(!params.skip_qc) {
             QC_WORKFLOW (
-                STARFUSION_WORKFLOW.out.ch_bam_sorted,
+                ch_starfusion_bams,
                 BUILD_REFERENCES.out.refflat,
                 BUILD_REFERENCES.out.fasta,
                 BUILD_REFERENCES.out.fai,

From bf316adfaf20b23543e3a6381ace9ad699acaab7 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 11:48:15 +0200
Subject: [PATCH 379/887] small tweaks on inputs

---
 subworkflows/local/build_references.nf        | 16 ++++-----
 .../local/fusioninspector_workflow.nf         |  7 +++-
 subworkflows/local/stringtie_workflow.nf      | 34 -------------------
 subworkflows/local/stringtie_workflow/main.nf |  7 ++--
 workflows/rnafusion.nf                        |  2 +-
 5 files changed, 20 insertions(+), 46 deletions(-)
 delete mode 100644 subworkflows/local/stringtie_workflow.nf

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 228dc58e0..ccccc547e 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -104,21 +104,21 @@ workflow BUILD_REFERENCES {
 
     def ch_salmon_index = Channel.empty()
     if (tools.contains("salmon")) {
-        if (!exists_not_empty(params.salmon_index) || !exists_not_empty(params.salmon_index_stub_check)){ // add condition for qc
+        if (!exists_not_empty(params.salmon_index)){ // add condition for qc
             GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
             ch_versions = ch_versions.mix(GFFREAD.out.versions)
             SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
             ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
             ch_salmon_index = SALMON_INDEX.out.index
         } else {
-            ch_salmon_index = Channel.fromPath({params.salmon_index})
+            ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.name], it] }
         }
     }
 
     def ch_starindex_ref = Channel.empty()
     def star_index_tools = tools.intersect(["starindex", "starfusion", "arriba", "ctatsplicing", "stringtie"])
     if (star_index_tools) {
-        if (!exists_not_empty(params.starindex_ref) || !exists_not_empty(params.starindex_ref_stub_check)) {
+        if (!exists_not_empty(params.starindex_ref)) {
             STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
             ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions)
             ch_starindex_ref = STAR_GENOMEGENERATE.out.index
@@ -150,20 +150,20 @@ workflow BUILD_REFERENCES {
 
     def ch_fusioncatcher_ref = Channel.empty()
     if (tools.contains("fusioncatcher")) {
-        if (!exists_not_empty(params.fusioncatcher_ref) || !exists_not_empty(params.fusioncatcher_ref_stub_check)) {
+        if (!exists_not_empty(params.fusioncatcher_ref)) {
                 FUSIONCATCHER_BUILD(params.genome_gencode_version)
                 ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
                 ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
         }
         else {
-            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref)
+            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { it -> [[id:it.name], it] }
         }
     }
 
     def ch_starfusion_ref = Channel.empty()
     def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing", "fusioninspector", "stringtie"])
     if (starfusion_tools) {
-        if (!exists_not_empty(params.starfusion_ref) || !exists_not_empty(params.starfusion_ref_stub_check)) {
+        if (!exists_not_empty(params.starfusion_ref)) {
             STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
@@ -178,13 +178,13 @@ workflow BUILD_REFERENCES {
             }
         }
         else {
-            ch_starfusion_ref = Channel.fromPath(params.starfusion_ref)
+            ch_starfusion_ref = Channel.fromPath(params.starfusion_ref).map { it -> [[id:it.name], it] }
         }
     }
 
     def ch_fusionreport_ref = Channel.empty()
     if (tools.contains("fusionreport")) {
-        if (!exists_not_empty(params.fusionreport_ref) || !exists_not_empty(params.fusionreport_ref_stub_check)) {
+        if (!exists_not_empty(params.fusionreport_ref)) {
             if (!params.no_cosmic && (!params.cosmic_username || !params.cosmic_passwd)) {
                 error('COSMIC username and/or password missing, this is needed to download the fusionreport reference')
             }
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index 56fd67321..754be50cc 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -54,7 +54,12 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
         if (!skip_vis) {
             ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(FUSIONINSPECTOR.out.tsv)
-            ARRIBA_VISUALISATION(ch_bam_sorted_indexed_fusions, ch_gtf, ch_arriba_ref_protein_domains, ch_arriba_ref_cytobands)
+            ARRIBA_VISUALISATION(
+                ch_bam_sorted_indexed_fusions,
+                ch_gtf,
+                ch_arriba_ref_protein_domains.map { it -> [[id:it.name], it]},
+                ch_arriba_ref_cytobands.map { it -> [[id:it.name], it]}
+            )
             ch_versions = ch_versions.mix(ARRIBA_VISUALISATION.out.versions)
             ch_arriba_visualisation = ARRIBA_VISUALISATION.out.pdf
         }
diff --git a/subworkflows/local/stringtie_workflow.nf b/subworkflows/local/stringtie_workflow.nf
deleted file mode 100644
index a9e05f304..000000000
--- a/subworkflows/local/stringtie_workflow.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-include { STRINGTIE_STRINGTIE }    from '../../modules/nf-core/stringtie/stringtie/main'
-include { STRINGTIE_MERGE }        from '../../modules/nf-core/stringtie/merge/main'
-
-
-workflow STRINGTIE_WORKFLOW {
-    take:
-        bam_sorted
-        ch_gtf
-
-    main:
-        ch_versions = Channel.empty()
-        ch_stringtie_gtf = Channel.empty()
-
-        STRINGTIE_STRINGTIE(bam_sorted, ch_gtf.map { _meta, gtf -> [ gtf ]})
-        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-        STRINGTIE_STRINGTIE
-            .out
-            .transcript_gtf
-            .map { it -> it[1] }
-            .set { stringtie_gtf }
-        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-
-        STRINGTIE_MERGE (stringtie_gtf, ch_gtf.map { _meta, gtf -> [ gtf ]})
-        ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
-        ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
-
-    emit:
-        stringtie_gtf = ch_stringtie_gtf.ifEmpty(null)
-        versions      = ch_versions
-
-    }
-
diff --git a/subworkflows/local/stringtie_workflow/main.nf b/subworkflows/local/stringtie_workflow/main.nf
index 3b27688c7..d9e65fd11 100644
--- a/subworkflows/local/stringtie_workflow/main.nf
+++ b/subworkflows/local/stringtie_workflow/main.nf
@@ -11,7 +11,10 @@ workflow STRINGTIE_WORKFLOW {
         ch_versions = Channel.empty()
         ch_stringtie_gtf = Channel.empty()
 
-        STRINGTIE_STRINGTIE(bam_sorted, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+        STRINGTIE_STRINGTIE(
+            bam_sorted.map { meta, bam, _bai -> [ meta, bam ]},
+            ch_chrgtf.map { _meta, gtf -> [ gtf ]}
+        )
         ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
 
         STRINGTIE_STRINGTIE
@@ -22,7 +25,7 @@ workflow STRINGTIE_WORKFLOW {
         ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
 
 
-        STRINGTIE_MERGE (stringtie_gtf, ch_chrgtf.map { meta, gtf -> [ gtf ]})
+        STRINGTIE_MERGE (stringtie_gtf, ch_chrgtf.map { _meta, gtf -> [ gtf ]})
         ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
         ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 857de11e4..f8bbde603 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -88,7 +88,7 @@ workflow RNAFUSION {
 
         if(tools.contains("salmon")) {
             SALMON_QUANT(
-                ch_reads.view(),
+                ch_reads,
                 BUILD_REFERENCES.out.salmon_index,
                 BUILD_REFERENCES.out.gtf.map{ it -> it[1] },
                 [],

From 8875f8cc7a6d96b48861fa460634eb457377833f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 11:56:18 +0200
Subject: [PATCH 380/887] update changelog

---
 CHANGELOG.md | 7 ++++---
 1 file changed, 4 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 687ec63d3..98185d06a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,7 +31,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `CTATSPLICING_PREPGENOMELIB` to update the starfusion genome library directory with a cancer splicing index. [#610](https://github.com/nf-core/rnafusion/pull/610)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
 - Add nf-test to local module: `ARRIBA_VISUALISATION`. [#625](https://github.com/nf-core/rnafusion/pull/625)
-- Added a new parameter `salmon` to enable the Salmon tool (default is `false`) [#]()
+- Added a new parameter `salmon` to enable the Salmon tool (default is `false`) [#645](https://github.com/nf-core/rnafusion/pull/645)
+- Added a new parameter `fusioninspector` to enable the Fusioninspector tool (default is `false`) [#645](https://github.com/nf-core/rnafusion/pull/645)
 
 ### Changed
 
@@ -52,8 +53,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
 - Update htslib and samtools version in `star/align` to 1.21 [#634](https://github.com/nf-core/rnafusion/pull/634)
 - `--run_fusioncatcher` back to `fusioncatcher` [#641](https://github.com/nf-core/rnafusion/pull/641)
-- Removed the `--fusioninspector_only` parameter, this is now the default behaviour of the pipeline [#]()
-- Renamed `--fastp_trim` to `--fastp` to be more uniform across with other tool parameters [#]()
+- Removed the `--fusioninspector_only` parameter, this is now the case when only `--fusionreport` is used together with `--skip_qc`, `--skip_vis` and `--skip_vcf` [#645](https://github.com/nf-core/rnafusion/pull/645)
+- Renamed `--fastp_trim` to `--fastp` to be more uniform across with other tool parameters [#645](https://github.com/nf-core/rnafusion/pull/645)
 
 ### Fixed
 

From b7f86364b44f1426048d3a472842bf5db9f0108f Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 11:52:08 +0000
Subject: [PATCH 381/887] fix some tests

---
 tests/test_build.nf.test  |  4 +++-
 tests/test_cosmic.nf.test |  2 +-
 tests/test_stub.nf.test   | 10 +++++-----
 workflows/rnafusion.nf    |  2 +-
 4 files changed, 10 insertions(+), 8 deletions(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index f6665777b..cd1977fd9 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -11,7 +11,9 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir               = "$outputDir"
+                outdir = "$outputDir"
+                salmon = true
+                fusioninspector = true
             }
         }
 
diff --git a/tests/test_cosmic.nf.test b/tests/test_cosmic.nf.test
index b4f2a3639..03370734a 100644
--- a/tests/test_cosmic.nf.test
+++ b/tests/test_cosmic.nf.test
@@ -12,7 +12,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir               = "$outputDir"
-                fastp_trim           = true
+                fastp                = true
             }
         }
 
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 065723e2f..1dfd79f97 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -12,9 +12,9 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir          = "$outputDir"
-                fastp_trim      = true
-                fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                outdir           = "$outputDir"
+                fastp            = true
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
             }
         }
 
@@ -44,8 +44,8 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir          = "$outputDir"
-                fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                outdir           = "$outputDir"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
             }
         }
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index f8bbde603..c147b4ff4 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -275,7 +275,7 @@ workflow RNAFUSION {
     //
 
     def ch_multiqc_output = Channel.empty()
-    if(!params.skip_qc) {
+    if(!params.skip_qc && !params.references_only) {
         ch_multiqc_config        = Channel.fromPath(
             "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
         ch_multiqc_custom_config = params.multiqc_config ?

From c4484465ea58126cf7871215e322e0048b9451eb Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 12:38:28 +0000
Subject: [PATCH 382/887] fix stub test snap

---
 tests/test_stub.nf.test.snap | 277 ++++++++++++++++++++++++++++++++++-
 1 file changed, 273 insertions(+), 4 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 206bc371e..076eed9df 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,7 +1,7 @@
 {
     "stub test no fastp trim": {
         "content": [
-            32,
+            36,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -9,21 +9,33 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.4.0"
+                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
                 },
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
                 "FUSIONCATCHER_BUILD": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONCATCHER_DETECT": {
                     "fusioncatcher": "1.33"
                 },
+                "FUSIONINSPECTOR": {
+                    "STAR-Fusion": null
+                },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
                 },
@@ -108,6 +120,8 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "arriba_visualisation",
+                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "ctatsplicing",
                 "ctatsplicing/arriba",
                 "ctatsplicing/arriba/test.cancer.introns",
@@ -133,15 +147,143 @@
                 "ctatsplicing/starfusion/test.igv.tracks",
                 "ctatsplicing/starfusion/test.introns",
                 "ctatsplicing/starfusion/test.introns.for_IGV.bed",
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
                 "fusioncatcher/human_v46",
                 "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
                 "fusioncatcher/test.fusioncatcher.log",
                 "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioninspector",
+                "fusioninspector/IGV_inputs",
+                "fusioninspector/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/IGV_inputs/cytoBand.txt",
+                "fusioninspector/IGV_inputs/test.ROI.bed",
+                "fusioninspector/IGV_inputs/test.bed",
+                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/IGV_inputs/test.fa",
+                "fusioninspector/IGV_inputs/test.fa.fai",
+                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/IGV_inputs/test.gtf",
+                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/IGV_inputs/tracks.json",
+                "fusioninspector/chckpts_dir",
+                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/chckpts_dir/blast_filter.ok",
+                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/chckpts_dir/cp_final.ok",
+                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/chckpts_dir/cytoband.ok",
+                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/chckpts_dir/final.abridged.ok",
+                "fusioninspector/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/chckpts_dir/microH.dat.ok",
+                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/chckpts_dir/run_STAR.ok",
+                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/fi_workdir",
+                "fusioninspector/fi_workdir/Log.final.out",
+                "fusioninspector/fi_workdir/Log.out",
+                "fusioninspector/fi_workdir/Log.progress.out",
+                "fusioninspector/fi_workdir/SJ.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome",
+                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/fi_workdir/microH.dat",
+                "fusioninspector/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/fi_workdir/star_align.ok",
+                "fusioninspector/fi_workdir/test.fa",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/fi_workdir/test.gtf",
+                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test.FusionInspector.log",
+                "fusioninspector/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -366,11 +508,11 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-19T09:52:40.993862676"
+        "timestamp": "2025-04-01T12:36:03.444372944"
     },
     "stub test with fastp trim": {
         "content": [
-            34,
+            36,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -378,6 +520,9 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.4.0"
+                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -399,6 +544,9 @@
                 "FUSIONCATCHER_DETECT": {
                     "fusioncatcher": "1.33"
                 },
+                "FUSIONINSPECTOR": {
+                    "STAR-Fusion": null
+                },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
                 },
@@ -483,6 +631,8 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "arriba_visualisation",
+                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "ctatsplicing",
                 "ctatsplicing/arriba",
                 "ctatsplicing/arriba/test.cancer.introns",
@@ -526,6 +676,125 @@
                 "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
                 "fusioncatcher/test.fusioncatcher.log",
                 "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioninspector",
+                "fusioninspector/IGV_inputs",
+                "fusioninspector/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/IGV_inputs/cytoBand.txt",
+                "fusioninspector/IGV_inputs/test.ROI.bed",
+                "fusioninspector/IGV_inputs/test.bed",
+                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/IGV_inputs/test.fa",
+                "fusioninspector/IGV_inputs/test.fa.fai",
+                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/IGV_inputs/test.gtf",
+                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/IGV_inputs/tracks.json",
+                "fusioninspector/chckpts_dir",
+                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/chckpts_dir/blast_filter.ok",
+                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/chckpts_dir/cp_final.ok",
+                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/chckpts_dir/cytoband.ok",
+                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/chckpts_dir/final.abridged.ok",
+                "fusioninspector/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/chckpts_dir/microH.dat.ok",
+                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/chckpts_dir/run_STAR.ok",
+                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/fi_workdir",
+                "fusioninspector/fi_workdir/Log.final.out",
+                "fusioninspector/fi_workdir/Log.out",
+                "fusioninspector/fi_workdir/Log.progress.out",
+                "fusioninspector/fi_workdir/SJ.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome",
+                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/fi_workdir/microH.dat",
+                "fusioninspector/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/fi_workdir/star_align.ok",
+                "fusioninspector/fi_workdir/test.fa",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/fi_workdir/test.gtf",
+                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test.FusionInspector.log",
+                "fusioninspector/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -750,6 +1019,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-19T09:51:49.095272949"
+        "timestamp": "2025-04-01T12:34:41.601673161"
     }
 }
\ No newline at end of file

From 59275b72e1906dec8dbc0a447721d556f1a0a097 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 15:37:24 +0200
Subject: [PATCH 383/887] remove missed stub check params

---
 nextflow.config      |  7 -------
 nextflow_schema.json | 30 ------------------------------
 2 files changed, 37 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index b82faf984..140ec507f 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -93,13 +93,6 @@ params {
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
     ctatsplicing_cancer_introns   = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
 
-    // Internal file presence checks
-    salmon_index_stub_check       = "${params.genomes_base}/salmon/salmon/complete_ref_lens.bin"
-    starindex_ref_stub_check      = "${params.genomes_base}/star/star/Genome"
-    fusionreport_ref_stub_check   = "${params.genomes_base}/fusion_report_db/mitelman.db"
-    fusioncatcher_ref_stub_check  = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}/ensembl_fully_overlapping_genes.txt"
-    starfusion_ref_stub_check     = "${params.genomes_base}/starfusion/Pfam-A.hmm"
-
     // Path to fusion outputs
     arriba_fusions                = null
     starfusion_fusions            = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index acd1758c9..c22cfe5b4 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -180,12 +180,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
-                "fusioncatcher_ref_stub_check": {
-                    "type": "string",
-                    "hidden": true,
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to file in fusioncatcher references"
-                },
                 "fusioninspector": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -217,12 +211,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusionreport references"
                 },
-                "fusionreport_ref_stub_check": {
-                    "type": "string",
-                    "hidden": true,
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to file in fusionreport references"
-                },
                 "hgnc_ref": {
                     "type": "string",
                     "format": "file-path",
@@ -253,12 +241,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to salmon index"
                 },
-                "salmon_index_stub_check": {
-                    "type": "string",
-                    "hidden": true,
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to file in salmon index"
-                },
                 "starfusion": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",
@@ -278,12 +260,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starfusion references"
                 },
-                "starfusion_ref_stub_check": {
-                    "type": "string",
-                    "hidden": true,
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to file in starfusion references"
-                },
                 "ctatsplicing_cancer_introns": {
                     "type": "string",
                     "format": "file-path",
@@ -303,12 +279,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starindex references"
                 },
-                "starindex_ref_stub_check": {
-                    "type": "string",
-                    "hidden": true,
-                    "fa_icon": "far fa-file-code",
-                    "description": "Path to file in starindex references"
-                },
                 "stringtie": {
                     "type": "boolean",
                     "fa_icon": "far fa-file-code",

From a756c4be680e5d41f50846362d3119c8913c9b4a Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 16:09:50 +0200
Subject: [PATCH 384/887] remove tool specific params and consolidate them in
 --tools

---
 conf/modules.config                           |  1 -
 main.nf                                       | 27 ++++----
 nextflow.config                               | 12 +---
 nextflow_schema.json                          | 64 ++-----------------
 subworkflows/local/build_references.nf        |  2 +-
 .../utils_nfcore_rnafusion_pipeline/main.nf   | 11 +---
 tests/test_build.nf.test                      |  3 +-
 tests/test_cosmic.nf.test                     |  2 +-
 tests/test_stub.nf.test                       |  2 +-
 9 files changed, 23 insertions(+), 101 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index d69aa7577..54489acbb 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -352,7 +352,6 @@ process {
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }
         publishDir = [
-            enabled: !params.ctatsplicing && !params.all,
             path: { "${params.genomes_base}/starfusion" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
diff --git a/main.nf b/main.nf
index 35d5817a7..3161d45a2 100644
--- a/main.nf
+++ b/main.nf
@@ -39,22 +39,19 @@ workflow {
         params.outdir,
     )
 
-    // A list of all tools available in the pipeline. Add tools here when a new tool has been added
-    def all_tools = [
-        "arriba",
-        "ctatsplicing",
-        "fusioncatcher",
-        "starindex",
-        "starfusion",
-        "stringtie",
-        "fusionreport",
-        "fastp",
-        "salmon",
-        "fusioninspector"
-    ]
+    def tools = params.tools.tokenize(",")
+    if (tools.contains("all")) {
+        def json = new groovy.json.JsonSlurper().parseText(file("${projectDir}/nextflow_schema.json").text)
+        def pattern = json.get('$defs')?.get('input_output_options')?.get('properties')?.get('tools')?.get('pattern')
+        if (!pattern) {
+            error("Could not fetch the allowed tools from the JSON schema, please check the code. If you see this as a pipeline user, please contact the developers instead.")
+        }
+        tools = pattern.replace('^((', "").replace(')?,?)*(?<!,)$', "").tokenize("|") - "all"
+    }
 
-    def tools = params.all ? all_tools : all_tools.findAll { tool ->
-        params.get(tool)
+    def profiles = workflow.profile
+    if ((profiles.contains("conda") || profiles.contains("mamba")) && (tools.contains("ctatsplicing"))) {
+        error("Conda or Mamba runs are not supported when ctatsplicing is in `--tools`")
     }
 
     //
diff --git a/nextflow.config b/nextflow.config
index 140ec507f..2fc31d3a9 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -52,17 +52,7 @@ params {
     fusioninspector_limitSjdbInsertNsj   = 1000000
 
     // Enable tools
-    all                        = false
-    arriba                     = false
-    ctatsplicing               = false
-    fusioncatcher              = false
-    starindex                  = false
-    starfusion                 = false
-    stringtie                  = false
-    fusionreport               = false
-    fusioninspector            = false
-    fastp                      = false
-    salmon                     = false
+    tools                      = null
 
     // Skip steps
     skip_qc                    = false
diff --git a/nextflow_schema.json b/nextflow_schema.json
index c22cfe5b4..ed9760755 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -36,7 +36,7 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": ["genomes_base", "outdir"],
+            "required": ["genomes_base", "outdir", "tools"],
             "properties": {
                 "input": {
                     "type": "string",
@@ -86,10 +86,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "gencode version"
                 },
-                "starfusion_build": {
-                    "type": "boolean",
+                "tools": {
+                    "type": "string",
+                    "description": "Comma-delimited list of tools to run",
                     "fa_icon": "far fa-file-code",
-                    "description": "If set, starfusion references are built from scratch instead of downloaded (default)"
+                    "pattern": "^((arriba|ctatsplicing|fusioncatcher|starfusion|stringtie|fusionreport|fastp|salmon|fusioninspector|all)?,?)*(?<!,)$"
                 },
                 "read_length": {
                     "type": "integer",
@@ -98,16 +99,6 @@
                     "default": 100,
                     "minimum": 1
                 },
-                "all": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build or run all references/analyses"
-                },
-                "arriba": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build or run arriba references/analyses"
-                },
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "format": "file-path",
@@ -149,16 +140,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Download references instead of building them (for fusioncatcher and starfusion)"
                 },
-                "ctatsplicing": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Run CTAT-splicing to detect abberant cancer splicing introns. Needs --arriba and/or --starfusion to run."
-                },
-                "fusioncatcher": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build or run fusioncatcher references/analyses"
-                },
                 "fusioncatcher_fusions": {
                     "type": "string",
                     "format": "file-path",
@@ -180,11 +161,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
-                "fusioninspector": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Run fusioninspector"
-                },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
@@ -200,11 +176,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to a fusion list file built with format GENE1--GENE2"
                 },
-                "fusionreport": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build fusionreport references"
-                },
                 "fusionreport_ref": {
                     "type": "string",
                     "format": "directory-path",
@@ -230,22 +201,12 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Use QIAGEN instead of SANGER to download COSMIC database"
                 },
-                "salmon": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Run Salmon"
-                },
                 "salmon_index": {
                     "type": "string",
                     "format": "directory-path",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to salmon index"
                 },
-                "starfusion": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build or run starfusion references/analyses"
-                },
                 "starfusion_fusions": {
                     "type": "string",
                     "format": "file-path",
@@ -268,22 +229,12 @@
                     "description": "Path to the cancer introns CSV file to create the CTAT-SPLICING reference with",
                     "default": "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
                 },
-                "starindex": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Build or run starindex references/analyses"
-                },
                 "starindex_ref": {
                     "type": "string",
                     "format": "directory-path",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to starindex references"
                 },
-                "stringtie": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Run stringtie analysis"
-                },
                 "tools_cutoff": {
                     "type": "integer",
                     "minimum": 1,
@@ -307,11 +258,6 @@
             "fa_icon": "fas fa-cut",
             "description": "Options to adjust read trimming criteria.",
             "properties": {
-                "fastp": {
-                    "type": "boolean",
-                    "description": "Preform fastp trimming of reads, default: false",
-                    "fa_icon": "fas fa-cut"
-                },
                 "trim_tail": {
                     "type": "integer",
                     "description": "The amount of bases to trim at the tail of each read, none will be trimmed by default",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index ccccc547e..2f93ef08f 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -116,7 +116,7 @@ workflow BUILD_REFERENCES {
     }
 
     def ch_starindex_ref = Channel.empty()
-    def star_index_tools = tools.intersect(["starindex", "starfusion", "arriba", "ctatsplicing", "stringtie"])
+    def star_index_tools = tools.intersect(["starfusion", "arriba", "ctatsplicing", "stringtie"])
     if (star_index_tools) {
         if (!exists_not_empty(params.starindex_ref)) {
             STAR_GENOMEGENERATE(ch_fasta, ch_gtf)
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 23184d336..e751b6430 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -156,16 +156,7 @@ def validateInputParameters() {
     genomeExistsError()
 
     if (params.no_cosmic) {
-            log.warn("Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`")
-    }
-
-    if (params.starfusion_build && !params.fusion_annot_lib) {
-            error("No fusion annotation library provided. `STARFUSION_BUILD` is unable to run.")
-    }
-
-    def profiles = workflow.profile
-    if ((profiles.contains("conda") || profiles.contains("mamba")) && (params.ctatsplicing || params.all)) {
-        error("Conda or Mamba runs are not supported when using --ctatsplicing or --all")
+        log.warn("Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`")
     }
 
 }
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index cd1977fd9..f2ab4ea4a 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -12,8 +12,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir = "$outputDir"
-                salmon = true
-                fusioninspector = true
+                tools  = "salmon,fusioninspector"
             }
         }
 
diff --git a/tests/test_cosmic.nf.test b/tests/test_cosmic.nf.test
index 03370734a..b547b2fe4 100644
--- a/tests/test_cosmic.nf.test
+++ b/tests/test_cosmic.nf.test
@@ -12,7 +12,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir               = "$outputDir"
-                fastp                = true
+                tools                = "fastp"
             }
         }
 
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 1dfd79f97..2311d989e 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -13,7 +13,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir           = "$outputDir"
-                fastp            = true
+                tools            = "fastp"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
             }
         }

From e76e3ad74fe311448ec558c375a4b19c10b7a6c4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 16:15:00 +0200
Subject: [PATCH 385/887] update changelog

---
 CHANGELOG.md | 15 +++++++++++----
 1 file changed, 11 insertions(+), 4 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 98185d06a..750826263 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,8 +31,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `CTATSPLICING_PREPGENOMELIB` to update the starfusion genome library directory with a cancer splicing index. [#610](https://github.com/nf-core/rnafusion/pull/610)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
 - Add nf-test to local module: `ARRIBA_VISUALISATION`. [#625](https://github.com/nf-core/rnafusion/pull/625)
-- Added a new parameter `salmon` to enable the Salmon tool (default is `false`) [#645](https://github.com/nf-core/rnafusion/pull/645)
-- Added a new parameter `fusioninspector` to enable the Fusioninspector tool (default is `false`) [#645](https://github.com/nf-core/rnafusion/pull/645)
 
 ### Changed
 
@@ -53,8 +51,17 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated documentation for fusion-report score calculation to reflect 80/20 weight distribution between tool detection and database hits [#620](https://github.com/nf-core/rnafusion/pull/620)
 - Update htslib and samtools version in `star/align` to 1.21 [#634](https://github.com/nf-core/rnafusion/pull/634)
 - `--run_fusioncatcher` back to `fusioncatcher` [#641](https://github.com/nf-core/rnafusion/pull/641)
-- Removed the `--fusioninspector_only` parameter, this is now the case when only `--fusionreport` is used together with `--skip_qc`, `--skip_vis` and `--skip_vcf` [#645](https://github.com/nf-core/rnafusion/pull/645)
-- Renamed `--fastp_trim` to `--fastp` to be more uniform across with other tool parameters [#645](https://github.com/nf-core/rnafusion/pull/645)
+- Removed `--fastp_trim`, `--arriba`, `--ctatsplicing`, `--fusioncatcher`, `--starfusion`, `--stringtie` and `--all` and replaced these parameters with the `--tools` parameter. This parameter takes a comma-delimited list of tool names to run for the pipeline and is a required parameter. Following tools are supported by this parameter [#645](https://github.com/nf-core/rnafusion/pull/645):
+  - `arriba`
+  - `ctatsplicing`
+  - `fusioncatcher`
+  - `starfusion`
+  - `stringtie`
+  - `fusionreport`
+  - `fastp`
+  - `salmon`
+  - `fusioninspector`
+  - `all` => This will automatically run all of the above tools
 
 ### Fixed
 

From 38018cd38bf587ecc2b5eb48d3abe15026e8f492 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 16:19:13 +0200
Subject: [PATCH 386/887] fix nf-core lint

---
 nextflow.config | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 2fc31d3a9..1e7e33e61 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -29,7 +29,6 @@ params {
     genomes_base               = "${params.outdir}/references"
     genome_gencode_version     = 46
     read_length                = 100
-    starfusion_build           = true
     genomes                    = [:]
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
     species                    = "human"

From 4f2ec253a67066c552d922d179589b4aac50e1e2 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 16:27:37 +0200
Subject: [PATCH 387/887] fix tests

---
 conf/test.config             |   2 +-
 tests/test_stub.nf.test      |  34 +--
 tests/test_stub.nf.test.snap | 515 +----------------------------------
 3 files changed, 4 insertions(+), 547 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index ba937e857..e29dc54af 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -16,7 +16,7 @@ params {
 
     // Input data
     input                  = params.pipelines_testdata_base_path +'rnafusion/testdata/human/samplesheet_valid.csv'
-    all                    = true
+    tools                  = "all"
     no_cosmic              = true
 }
 
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 2311d989e..8b5a0f569 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -8,39 +8,7 @@ nextflow_pipeline {
     tag "pipeline_rnafusion"
     tag "test_stub"
 
-    test("stub test with fastp trim") {
-
-        when {
-            params {
-                outdir           = "$outputDir"
-                tools            = "fastp"
-                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    // stable_path // TODO uncomment once -stub is removed
-                ).match() }
-            )
-        }
-    }
-
-    test("stub test no fastp trim") {
+    test("stub test all") {
 
         when {
             params {
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 076eed9df..33c923597 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "stub test no fastp trim": {
+    "stub test all": {
         "content": [
             36,
             {
@@ -508,517 +508,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-01T12:36:03.444372944"
-    },
-    "stub test with fastp trim": {
-        "content": [
-            36,
-            {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
-                },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.4.0"
-                },
-                "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "CTATSPLICING_STARTOCANCERINTRONS": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "FASTP": {
-                    "fastp": "0.23.4"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FASTQC_FOR_FASTP": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "1.33"
-                },
-                "FUSIONCATCHER_DETECT": {
-                    "fusioncatcher": "1.33"
-                },
-                "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
-                },
-                "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
-                },
-                "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": null
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GFFREAD": {
-                    "gffread": "0.12.7"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "HGNC_DOWNLOAD": {
-                    "wget": null
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SALMON_INDEX": {
-                    "salmon": "1.10.3"
-                },
-                "SALMON_QUANT": {
-                    "salmon": "1.10.3"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "SAMTOOLS_INDEX_FOR_STARFUSION": {
-                    "samtools": 1.21
-                },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
-                },
-                "STARFUSION_BUILD": {
-                    "STAR-Fusion": "1.14.0"
-                },
-                "STAR_FOR_ARRIBA": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "STAR_FOR_STARFUSION": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.3"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
-            [
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
-                "ctatsplicing",
-                "ctatsplicing/arriba",
-                "ctatsplicing/arriba/test.cancer.introns",
-                "ctatsplicing/arriba/test.cancer.introns.prelim",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/arriba/test.chckpts",
-                "ctatsplicing/arriba/test.ctat-splicing.igv.html",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/arriba/test.igv.tracks",
-                "ctatsplicing/arriba/test.introns",
-                "ctatsplicing/arriba/test.introns.for_IGV.bed",
-                "ctatsplicing/starfusion",
-                "ctatsplicing/starfusion/test.cancer.introns",
-                "ctatsplicing/starfusion/test.cancer.introns.prelim",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/starfusion/test.chckpts",
-                "ctatsplicing/starfusion/test.ctat-splicing.igv.html",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/starfusion/test.igv.tracks",
-                "ctatsplicing/starfusion/test.introns",
-                "ctatsplicing/starfusion/test.introns.for_IGV.bed",
-                "fastp",
-                "fastp/test.fastp.html",
-                "fastp/test.fastp.json",
-                "fastp/test.fastp.log",
-                "fastp/test_1.fastp.fastq.gz",
-                "fastp/test_2.fastp.fastq.gz",
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
-                "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
-                "fusioninspector",
-                "fusioninspector/IGV_inputs",
-                "fusioninspector/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/IGV_inputs/cytoBand.txt",
-                "fusioninspector/IGV_inputs/test.ROI.bed",
-                "fusioninspector/IGV_inputs/test.bed",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/IGV_inputs/test.fa",
-                "fusioninspector/IGV_inputs/test.fa.fai",
-                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/IGV_inputs/test.gtf",
-                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/IGV_inputs/tracks.json",
-                "fusioninspector/chckpts_dir",
-                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/chckpts_dir/blast_filter.ok",
-                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/chckpts_dir/cp_final.ok",
-                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/chckpts_dir/cytoband.ok",
-                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/chckpts_dir/final.abridged.ok",
-                "fusioninspector/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/chckpts_dir/microH.dat.ok",
-                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/chckpts_dir/run_STAR.ok",
-                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/fi_workdir",
-                "fusioninspector/fi_workdir/Log.final.out",
-                "fusioninspector/fi_workdir/Log.out",
-                "fusioninspector/fi_workdir/Log.progress.out",
-                "fusioninspector/fi_workdir/SJ.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome",
-                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/fi_workdir/microH.dat",
-                "fusioninspector/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/fi_workdir/star_align.ok",
-                "fusioninspector/fi_workdir/test.fa",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/fi_workdir/test.gtf",
-                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.fusion_inspector_web.html",
-                "fusionreport",
-                "fusionreport/test",
-                "fusionreport/test/AAA_BBB.html",
-                "fusionreport/test/test.fusionreport.tsv",
-                "fusionreport/test/test.fusionreport_filtered.tsv",
-                "fusionreport/test/test.fusions.csv",
-                "fusionreport/test/test.fusions.json",
-                "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
-                "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
-                "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
-                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/refGene.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
-                "salmon",
-                "salmon/test",
-                "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv",
-                "stringtie",
-                "stringtie/[:]",
-                "stringtie/[:]/stringtie.merged.gtf",
-                "stringtie/test.ballgown",
-                "stringtie/test.coverage.gtf",
-                "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-01T12:34:41.601673161"
+        "timestamp": "2025-04-01T16:25:22.357112178"
     }
 }
\ No newline at end of file

From d61389eda3bacbd89598eb3d0ae5034611f4f004 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 1 Apr 2025 16:32:48 +0200
Subject: [PATCH 388/887] update test_build config

---
 conf/test_build.config   | 8 +-------
 tests/test_build.nf.test | 1 -
 2 files changed, 1 insertion(+), 8 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index 971e386ae..511b83657 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -37,16 +37,10 @@ params {
     references_only            = true
     input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
     no_cosmic                  = true
-    all                        = false
-    arriba                     = true
-    fusioncatcher              = false
-    starfusion                 = true
+    tools                      = "arriba,starfusion,fusionreport,salmon,fusioninspector"
     fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'
     gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
-    fusionreport               = true
 
-    skip_salmon_index          = true
-    starfusion_build           = true
     fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
 
 }
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index f2ab4ea4a..6fb5cc32a 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -12,7 +12,6 @@ nextflow_pipeline {
         when {
             params {
                 outdir = "$outputDir"
-                tools  = "salmon,fusioninspector"
             }
         }
 

From 78d37f3f2c83e86364cdde8d729f2795333277c4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 13:49:48 +0200
Subject: [PATCH 389/887] add tool tests

---
 nextflow_schema.json         |    5 -
 tests/test_stub.nf.test      |  288 ++++++
 tests/test_stub.nf.test.snap | 1678 ++++++++++++++++++++++++++++++++++
 3 files changed, 1966 insertions(+), 5 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index ed9760755..1f40b5ad5 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -22,11 +22,6 @@
                 "skip_vis": {
                     "type": "boolean",
                     "description": "Skip visualisation steps"
-                },
-                "skip_salmon_index": {
-                    "type": "boolean",
-                    "description": "Skip salmon index generation step",
-                    "hidden": true
                 }
             },
             "fa_icon": "fas fa-fast-forward"
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 8b5a0f569..562908322 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -38,4 +38,292 @@ nextflow_pipeline {
             )
         }
     }
+
+    test("stub test arriba") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "arriba"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test ctatsplicing") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "ctatsplicing"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fusioncatcher") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fusioncatcher"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test starfusion") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "starfusion"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test stringtie") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "stringtie"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fusionreport") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fusionreport,arriba,starfusion,fusioncatcher"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fastp") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fastp"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test salmon") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "salmon"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fusioninspector") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fusioninspector,arriba,fusionreport"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
 }
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 33c923597..d673b42de 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,4 +1,1682 @@
 {
+    "stub test starfusion": {
+        "content": [
+            16,
+            {
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX_FOR_STARFUSION": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_FOR_STARFUSION": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
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+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "star_for_arriba",
+                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
+                "star_for_arriba/test.Aligned.unsort.out.bam",
+                "star_for_arriba/test.Chimeric.out.junction",
+                "star_for_arriba/test.Log.final.out",
+                "star_for_arriba/test.Log.out",
+                "star_for_arriba/test.Log.progress.out",
+                "star_for_arriba/test.ReadsPerGene.out.tab",
+                "star_for_arriba/test.SJ.out.tab",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_arriba/test.out.sam",
+                "star_for_arriba/test.sortedByCoord.out.bam",
+                "star_for_arriba/test.tab",
+                "star_for_arriba/test.toTranscriptome.out.bam",
+                "star_for_arriba/test.unmapped_1.fastq.gz",
+                "star_for_arriba/test.unmapped_2.fastq.gz",
+                "star_for_arriba/testXd.out.bam",
+                "star_for_starfusion",
+                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
+                "star_for_starfusion/test.Aligned.unsort.out.bam",
+                "star_for_starfusion/test.Chimeric.out.junction",
+                "star_for_starfusion/test.Log.final.out",
+                "star_for_starfusion/test.Log.out",
+                "star_for_starfusion/test.Log.progress.out",
+                "star_for_starfusion/test.ReadsPerGene.out.tab",
+                "star_for_starfusion/test.SJ.out.tab",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
+                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
+                "star_for_starfusion/test.out.sam",
+                "star_for_starfusion/test.sortedByCoord.out.bam",
+                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
+                "star_for_starfusion/test.tab",
+                "star_for_starfusion/test.toTranscriptome.out.bam",
+                "star_for_starfusion/test.unmapped_1.fastq.gz",
+                "star_for_starfusion/test.unmapped_2.fastq.gz",
+                "star_for_starfusion/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-02T13:30:26.192389023"
+    },
+    "stub test fastp": {
+        "content": [
+            10,
+            {
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-02T13:31:22.055312226"
+    },
+    "stub test fusioncatcher": {
+        "content": [
+            10,
+            {
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "1.33"
+                },
+                "FUSIONCATCHER_DETECT": {
+                    "fusioncatcher": "1.33"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fusioncatcher",
+                "fusioncatcher/human_v46",
+                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-02T13:26:15.002394926"
+    },
     "stub test all": {
         "content": [
             36,

From f038c0b61f31112a6a34e23fb1cdd36b532c043b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 13:52:00 +0200
Subject: [PATCH 390/887] fix lint

---
 nextflow.config | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 1e7e33e61..bf778cac9 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -57,7 +57,6 @@ params {
     skip_qc                    = false
     skip_vis                   = false
     skip_vcf                   = false
-    skip_salmon_index          = false
 
     // Download references option
     download_refs              = false

From 9460cd6edda1949f002e666f5b179078c2b25901 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Wed, 2 Apr 2025 14:38:40 +0200
Subject: [PATCH 391/887] Update subworkflows/local/build_references.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 subworkflows/local/build_references.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 2f93ef08f..0fd01eab3 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -35,7 +35,7 @@ include { GFFREAD }                         from '../../modules/nf-core/gffread/
 workflow BUILD_REFERENCES {
 
     take:
-    tools // list of all the tools to create references from
+    tools // list of all the tools to create references for
 
     main:
     def ch_versions = Channel.empty()

From f92a30ed8707f2b69a1f30d1a7bedea3389813fa Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Wed, 2 Apr 2025 14:39:21 +0200
Subject: [PATCH 392/887] Update subworkflows/local/build_references.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 subworkflows/local/build_references.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 0fd01eab3..97a0e172b 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -63,6 +63,7 @@ workflow BUILD_REFERENCES {
 
     def ch_hgnc_date = Channel.empty()
     def ch_hgnc_ref  = Channel.empty()
+//TODO: unify as if(tools.contains("fusioninspector")) once nextflow bug fixed
     def run_fusioninspector = tools.contains("fusioninspector")
     if(run_fusioninspector) {
         if ((!exists_not_empty(params.hgnc_ref) || !exists_not_empty(params.hgnc_date)) && !params.skip_vcf){

From afeea03c79f32797803fdac0b6a288575eb33050 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Wed, 2 Apr 2025 14:40:04 +0200
Subject: [PATCH 393/887] Update subworkflows/local/build_references.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 subworkflows/local/build_references.nf | 19 +++++++++++--------
 1 file changed, 11 insertions(+), 8 deletions(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 97a0e172b..01aa1cba2 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -105,14 +105,17 @@ workflow BUILD_REFERENCES {
 
     def ch_salmon_index = Channel.empty()
     if (tools.contains("salmon")) {
-        if (!exists_not_empty(params.salmon_index)){ // add condition for qc
-            GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
-            ch_versions = ch_versions.mix(GFFREAD.out.versions)
-            SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
-            ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
-            ch_salmon_index = SALMON_INDEX.out.index
-        } else {
-            ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.name], it] }
+        if (!params.skip_qc) {
+            if (!exists_not_empty(params.salmon_index)){
+                GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
+                ch_versions = ch_versions.mix(GFFREAD.out.versions)
+            
+                SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
+                ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
+                ch_salmon_index = SALMON_INDEX.out.index
+            } else {
+                ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.name], it] }
+            }
         }
     }
 

From 584f0009841e10ca46a2c001e72ca9aaeb0210f1 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 14:42:21 +0200
Subject: [PATCH 394/887] remove fusioninspector from arriba tools

---
 subworkflows/local/build_references.nf | 5 ++---
 1 file changed, 2 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 01aa1cba2..18c16c93a 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -109,7 +109,7 @@ workflow BUILD_REFERENCES {
             if (!exists_not_empty(params.salmon_index)){
                 GFFREAD(ch_gtf, ch_fasta.map{ it -> it[1] })
                 ch_versions = ch_versions.mix(GFFREAD.out.versions)
-            
+
                 SALMON_INDEX(ch_fasta.map{ it -> it[1] }, GFFREAD.out.gffread_fasta.map{ it -> it[1] })
                 ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
                 ch_salmon_index = SALMON_INDEX.out.index
@@ -135,8 +135,7 @@ workflow BUILD_REFERENCES {
     def ch_arriba_ref_cytobands       = Channel.empty()
     def ch_arriba_ref_known_fusions   = Channel.empty()
     def ch_arriba_ref_protein_domains = Channel.empty()
-    def arriba_tools = tools.intersect(["arriba", "fusioninspector"])
-    if (arriba_tools) {
+    if (tools.contains("arriba")) {
         if (!exists_not_empty(params.arriba_ref_blacklist) || !exists_not_empty(params.arriba_ref_known_fusions) || !exists_not_empty(params.arriba_ref_protein_domains)) {
             ARRIBA_DOWNLOAD(params.genome)
             ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)

From 8ead7fd7f52c1c72a9bfb1fe3cd2b802b8effc84 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Wed, 2 Apr 2025 14:45:02 +0200
Subject: [PATCH 395/887] Update subworkflows/local/build_references.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 subworkflows/local/build_references.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 18c16c93a..626e6aa55 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -164,6 +164,7 @@ workflow BUILD_REFERENCES {
     }
 
     def ch_starfusion_ref = Channel.empty()
+     //TODO update list once alignment is done in a single step
     def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing", "fusioninspector", "stringtie"])
     if (starfusion_tools) {
         if (!exists_not_empty(params.starfusion_ref)) {

From ee0df1789a0ffb542d4abb68919a3f914ea111e1 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 14:57:18 +0200
Subject: [PATCH 396/887] add cytobands to arriba check

---
 subworkflows/local/build_references.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 18c16c93a..21ed75c93 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -136,7 +136,7 @@ workflow BUILD_REFERENCES {
     def ch_arriba_ref_known_fusions   = Channel.empty()
     def ch_arriba_ref_protein_domains = Channel.empty()
     if (tools.contains("arriba")) {
-        if (!exists_not_empty(params.arriba_ref_blacklist) || !exists_not_empty(params.arriba_ref_known_fusions) || !exists_not_empty(params.arriba_ref_protein_domains)) {
+        if (!exists_not_empty(params.arriba_ref_blacklist) || !exists_not_empty(params.arriba_ref_known_fusions) || !exists_not_empty(params.arriba_ref_protein_domains) || !exists_not_empty(params.arriba_ref_cytobands)) {
             ARRIBA_DOWNLOAD(params.genome)
             ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)
             ch_arriba_ref_blacklist       = ARRIBA_DOWNLOAD.out.blacklist

From 7e2c221c6595938e59514f59d33cc3e98b1f5543 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 14:57:24 +0200
Subject: [PATCH 397/887] update usage docs

---
 docs/usage.md | 39 +++++++++++++++++++++------------------
 1 file changed, 21 insertions(+), 18 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index f0afcebb9..4ee121b19 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -32,7 +32,7 @@ The rnafusion pipeline needs references for the fusion detection tools, so downl
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --references_only --all \
+  --references_only --tools all \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <PATH/TO/REFERENCES>
@@ -43,7 +43,7 @@ References for each tools can also be downloaded separately with:
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --references_only --<tool1> --<tool2> ... \
+  --references_only --tools <comma-separated-list-of-tools> \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH>
@@ -64,15 +64,15 @@ Use credentials from QIAGEN and add `--qiagen`
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --references_only --<tool1> --<tool2> ... \
+  --references_only --tools <comma-separated-list-of-tools> \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH> --qiagen
 ```
 
-#### STAR-Fusion references downloaded vs built
+<!-- #### STAR-Fusion references downloaded vs built
 
-By default STAR-Fusion references are **built**. You can also download them from [CTAT](https://github.com/NCIP/Trinity_CTAT/wiki) by using the flag `--starfusion_build FALSE` for both reference building and fusion detection. This allows more flexibility for different organisms but **be aware that STAR-Fusion reference download is not recommended as not fully tested!**
+By default STAR-Fusion references are **built**. You can also download them from [CTAT](https://github.com/NCIP/Trinity_CTAT/wiki) by using the flag `--starfusion_build FALSE` for both reference building and fusion detection. This allows more flexibility for different organisms but **be aware that STAR-Fusion reference download is not recommended as not fully tested!** -->
 
 #### Issues with building references
 
@@ -136,23 +136,23 @@ As you can see above for multiple runs of the same sample, the `sample` name has
 
 ### Starting commands
 
-The pipeline can either be run using all fusion detection tools or specifying individual tools. Visualisation tools will be run on all fusions detected. To run all tools (`arriba`, `fusioncatcher`, `starfusion`, `stringtie`, `ctat-splicing`) use the `--all` parameter:
+The pipeline can either be run using all fusion detection tools or by specifying individual tools. Visualisation tools will be run on all fusions detected. To run all tools (`arriba`, `ctatsplicing`, `fusioncatcher`, `starfusion`, `stringtie`, `fusionreport`, `fastp`, `salmon`, `fusioninspector`) use the `all` option for the `--tools` parameter:
 
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --all \
+  --tools all \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH>
 ```
 
-To run only a specific detection tool use: `--tool`:
+To run only a set of specific tools use `--tools` with a comma separated list of requested tools:
 
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --<tool1> --<tool2> ... \
+  --tools <comma-separated-list-of-tools> \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH>
@@ -160,7 +160,7 @@ nextflow run nf-core/rnafusion \
 
 If you are not covered by the research COSMIC license and want to avoid using COSMIC, you can provide the additional option `--no_cosmic`.
 
-> **IMPORTANT: Either `--all` or `--<tool>`** is necessary to run detection tools
+> **IMPORTANT: `--tools`** is necessary to run detection tools
 
 `--genomes_base` should be the path to the directory containing the folder `references/` that was built with `--references_only`.
 
@@ -205,12 +205,12 @@ Supported genome is currently only GRCh38.
 
 #### Trimming
 
-When the flag `--fastp_trim` is used, `fastp` is used to provide all tools with trimmed reads. Quality and adapter trimming by default. In addition, tail trimming and adapter_fastq specification are possible. Example usage:
+When the flag `fastp` tool is used in `--tools`, `fastp` is used to provide all tools with trimmed reads. Quality and adapter trimming by default. In addition, tail trimming and adapter_fastq specification are possible. Example usage:
 
 ```bash
 nextflow run nf-core/rnafusion \
 -profile <docker/singularity/.../institute> \
---<tool1> --<tool2> ... \
+--tools fastp,... \
 --input <SAMPLE_SHEET.CSV> \
 --genomes_base <PATH/TO/REFERENCES> \
 --outdir <OUTPUT/PATH> \
@@ -224,7 +224,7 @@ nextflow run nf-core/rnafusion \
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --<tool1> --<tool2> ... \
+  --tools <comma-separated-list-of-tools> \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH>
@@ -238,7 +238,7 @@ nextflow run nf-core/rnafusion \
 ```bash
 nextflow run nf-core/rnafusion \
   -profile <docker/singularity/.../institute> \
-  --<tool1> --<tool2> ... \
+  --tools <comma-separated-list-of-tools> \
   --input <SAMPLE_SHEET.CSV> \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH>
@@ -259,7 +259,10 @@ FusionInspector can be run as a standalone with:
 ```bash
 nextflow run nf-core/rnafusion \
 -profile <docker/singularity/.../institute> \
---fusioninspector_only \
+--tools fusioninspector \
+--skip_qc \
+--skip_vis \
+--skip_vcf \
 --fusioninspector_fusions <PATH_TO_CUSTOM_FUSION_FILE> \
 --input <SAMPLE_SHEET.CSV> \
 --outdir <PATH>
@@ -278,7 +281,7 @@ GENE3--GENE4
 nextflow run nf-core/rnafusion \
 -profile <docker/singularity/.../institute> \
 --skip_qc \
---all OR <--tool>
+--tools <comma-separated-list-of-tools> \
 --input <SAMPLE_SHEET.CSV> \
 --genomes_base <PATH/TO/REFERENCES> \
 --outdir <PATH>
@@ -292,7 +295,7 @@ This will skip all QC-related processes (picard metrics collection)
 nextflow run nf-core/rnafusion \
 -profile <docker/singularity/.../institute> \
 --skip_vis \
---all OR <--tool>
+--tools <comma-separated-list-of-tools> \
 --input <SAMPLE_SHEET.CSV> \
 --genomes_base <PATH/TO/REFERENCES> \
 --outdir <PATH>
@@ -302,7 +305,7 @@ This will skip all visualisation processes, including `fusion-report`, `FusionIn
 
 #### Optional manual feed-in of fusion files
 
-It is possible to give the output of each tool manually using the argument: `--<tool>_fusions PATH/TO/FUSION/FILE`: this feature need more testing, don't hesitate to open an issue if you encounter problems.
+It is possible to give the output of each fusion detection tool manually using the argument: `--<tool>_fusions PATH/TO/FUSION/FILE`: this feature needs more testing, don't hesitate to open an issue if you encounter problems.
 
 #### Set different `--limitSjdbInsertNsj` parameter
 

From 02b68f1bbba12a0df34c31107e8671f391b2705c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Wed, 2 Apr 2025 14:58:31 +0200
Subject: [PATCH 398/887] Update workflows/rnafusion.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index c147b4ff4..c1cd21093 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -51,7 +51,7 @@ workflow RNAFUSION {
     BUILD_REFERENCES(tools)
     ch_versions = ch_versions.mix(BUILD_REFERENCES.out.versions)
 
-    if (!params.references_only) { // TODO: Remove this temporary parameter when we have a full-working GitHub nf-test
+    if (!params.references_only) {
 
         //
         // QC from FASTQ files

From a021d673257f94811f026fd4c6c5b90abe2e0026 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Wed, 2 Apr 2025 14:58:50 +0200
Subject: [PATCH 399/887] Update workflows/rnafusion.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index c1cd21093..54b0b1ee5 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -86,7 +86,7 @@ workflow RNAFUSION {
             ch_reads = ch_samplesheet
         }
 
-        if(tools.contains("salmon")) {
+        if(tools.contains("salmon") && !params.skip_qc) {
             SALMON_QUANT(
                 ch_reads,
                 BUILD_REFERENCES.out.salmon_index,

From 8a04a6f0dab12e4cf3cf1ce0ba1d0c310d9dee9a Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 15:02:47 +0200
Subject: [PATCH 400/887] fix pre-commit

---
 subworkflows/local/build_references.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 399b7c3d0..1e4577c16 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -164,7 +164,7 @@ workflow BUILD_REFERENCES {
     }
 
     def ch_starfusion_ref = Channel.empty()
-     //TODO update list once alignment is done in a single step
+    //TODO update list once alignment is done in a single step
     def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing", "fusioninspector", "stringtie"])
     if (starfusion_tools) {
         if (!exists_not_empty(params.starfusion_ref)) {

From 9d6a5f690a32074264b8ad41a7c4330cd4f87af5 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 15:49:55 +0200
Subject: [PATCH 401/887] improve exists_not_empty to also work for directories

---
 subworkflows/local/build_references.nf | 19 ++++++++++++++++++-
 1 file changed, 18 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 1e4577c16..cf1965d8c 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -25,6 +25,7 @@ include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../modules/nf-core/gatk4/cr
 include { GATK4_BEDTOINTERVALLIST }         from '../../modules/nf-core/gatk4/bedtointervallist/main'
 include { SALMON_INDEX }                    from '../../modules/nf-core/salmon/index/main'
 include { GFFREAD }                         from '../../modules/nf-core/gffread/main'
+include { getFileSuffix } from '../../modules/nf-core/cat/cat/main.nf'
 
 /*
 ========================================================================================
@@ -228,5 +229,21 @@ workflow BUILD_REFERENCES {
 
 def exists_not_empty(path) {
     def path_to_check = file(path as String)
-    return path_to_check.exists() && !path_to_check.isEmpty()
+    // Return false if the path does not exist
+    if(!path_to_check.exists()) {
+        return false
+    }
+
+    // Check if the file is not a directory and return whether it's empty or not
+    if(!path_to_check.toFile().isDirectory()) {
+        return !path_to_check.isEmpty()
+    }
+
+    // Get the first file in a directory and return whether it is empty or not
+    def first_file = null
+    path_to_check.toFile().eachFileRecurse(groovy.io.FileType.FILES) { file ->
+        first_file = file
+        return
+    }
+    return !first_file.toPath().isEmpty()
 }

From 649b315fff5d9d9f4b62a7ac6cd758c62de66724 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 15:57:24 +0200
Subject: [PATCH 402/887] add an extra check for --fusion_annot_lib

---
 subworkflows/local/build_references.nf | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index cf1965d8c..036401de7 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -169,6 +169,9 @@ workflow BUILD_REFERENCES {
     def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing", "fusioninspector", "stringtie"])
     if (starfusion_tools) {
         if (!exists_not_empty(params.starfusion_ref)) {
+            if(!params.fusion_annot_lib) {
+                error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
+            }
             STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {

From 1edbf08a0fa6f44edc4479c9413424005ab14319 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 16:08:00 +0200
Subject: [PATCH 403/887] https URLs can't be directories

---
 subworkflows/local/build_references.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 036401de7..0e3979b23 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -237,8 +237,8 @@ def exists_not_empty(path) {
         return false
     }
 
-    // Check if the file is not a directory and return whether it's empty or not
-    if(!path_to_check.toFile().isDirectory()) {
+    // Check if the file is not a directory or is a URL and return whether it's empty or not
+    if(path_to_check.startswith("http://") || path_to_check.startswith("https://") || !path_to_check.toFile().isDirectory()) {
         return !path_to_check.isEmpty()
     }
 

From 1624397ed959be713dd3a8253fa30add544bd8ff Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 16:12:21 +0200
Subject: [PATCH 404/887] fix typo

---
 subworkflows/local/build_references.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 0e3979b23..a2b6ac4b9 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -238,7 +238,7 @@ def exists_not_empty(path) {
     }
 
     // Check if the file is not a directory or is a URL and return whether it's empty or not
-    if(path_to_check.startswith("http://") || path_to_check.startswith("https://") || !path_to_check.toFile().isDirectory()) {
+    if(path_to_check.startsWith("http://") || path_to_check.startsWith("https://") || !path_to_check.toFile().isDirectory()) {
         return !path_to_check.isEmpty()
     }
 

From 1f247e5d852cf50345f6166b39d28a3cd8bb0673 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 2 Apr 2025 16:28:16 +0200
Subject: [PATCH 405/887] fix url check

---
 subworkflows/local/build_references.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index a2b6ac4b9..b50e04d3f 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -238,7 +238,8 @@ def exists_not_empty(path) {
     }
 
     // Check if the file is not a directory or is a URL and return whether it's empty or not
-    if(path_to_check.startsWith("http://") || path_to_check.startsWith("https://") || !path_to_check.toFile().isDirectory()) {
+    def is_url = ["https://", "ftp://", "http://"].findAll { it -> path.startsWith(it) }.size() > 0
+    if(is_url || !path_to_check.toFile().isDirectory()) {
         return !path_to_check.isEmpty()
     }
 

From 8ad58419f6af9794a82a3fe52179429c437d78b5 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 3 Apr 2025 14:50:42 +0200
Subject: [PATCH 406/887] fix issues introduced with the merge

---
 subworkflows/local/arriba_workflow/main.nf    | 10 +++---
 .../local/ctatsplicing_workflow/main.nf       |  6 ++--
 subworkflows/local/qc_workflow/main.nf        |  6 ++--
 .../local/starfusion_workflow/main.nf         | 11 ++----
 .../starfusion_workflow/tests/main.nf.test    |  4 ---
 subworkflows/local/stringtie_workflow/main.nf |  2 +-
 workflows/rnafusion.nf                        | 34 +++++++------------
 7 files changed, 27 insertions(+), 46 deletions(-)

diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
index 14af59ba1..7580133b8 100644
--- a/subworkflows/local/arriba_workflow/main.nf
+++ b/subworkflows/local/arriba_workflow/main.nf
@@ -13,15 +13,14 @@ workflow ARRIBA_WORKFLOW {
 
     main:
 
-        def ch_versions   = Channel.empty()
-
-        if ( arriba_fusions ) {
+        def ch_versions           = Channel.empty()
+        def ch_arriba_fusions     = Channel.empty()
+        def ch_arriba_fusion_fail = Channel.empty()
 
+        if (arriba_fusions) {
             ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
                 .map { it -> [ it[0], it[2] ] }
-
         } else {
-
             ARRIBA_ARRIBA (
                 reads,
                 ch_fasta,
@@ -31,7 +30,6 @@ workflow ARRIBA_WORKFLOW {
                 ch_arriba_ref_cytobands,
                 ch_arriba_ref_protein_domains
             )
-
             ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
 
             ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
index f8b2d3592..7f83802e0 100644
--- a/subworkflows/local/ctatsplicing_workflow/main.nf
+++ b/subworkflows/local/ctatsplicing_workflow/main.nf
@@ -4,7 +4,7 @@ workflow CTATSPLICING_WORKFLOW {
     take:
     split_junctions // [ val(meta), path(split_junctions.SJ.out.tab) ]
     junctions       // [ val(meta), path(junctions.Chimeric.out.junction) ]
-    aligned_bams    // [ val(meta), path(aligned_bams.Aligned.sortedByCoord.out.bam) ]
+    aligned_bams    // [ val(meta), path(aligned_bams.Aligned.sortedByCoord.out.bam), path(aligned_bams.Aligned.sortedByCoord.out.bam.bai) ]
     ctat_genome_lib // [ val(meta2), path(path/to/ctat_genome_lib) ]
 
     main:
@@ -13,8 +13,8 @@ workflow CTATSPLICING_WORKFLOW {
     def ch_ctatsplicing_input = split_junctions
         .join(junctions, failOnMismatch:true, failOnDuplicate:true)
         .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
-        .map { meta, split_junction, junction, bam ->
-            [ meta, split_junction, junction, bam, [] ]
+        .map { meta, split_junction, junction, bam, bai ->
+            [ meta, split_junction, junction, bam, bai ]
         }
 
     CTATSPLICING_STARTOCANCERINTRONS(
diff --git a/subworkflows/local/qc_workflow/main.nf b/subworkflows/local/qc_workflow/main.nf
index 4924b633b..9d9833f0a 100644
--- a/subworkflows/local/qc_workflow/main.nf
+++ b/subworkflows/local/qc_workflow/main.nf
@@ -18,7 +18,7 @@ workflow QC_WORKFLOW {
         ch_versions = Channel.empty()
 
         PICARD_COLLECTRNASEQMETRICS(
-            ch_bam_sorted.map { meta, bam, _bai -> [ meta, bam ]},
+            ch_bam_sorted,
             ch_refflat.map{ _meta, refflat -> [ refflat ] },
             ch_fasta.map{ _meta, fasta -> [ fasta ] },
             ch_rrna_interval.map{ _meta, intervals -> [ intervals ] }.ifEmpty([])
@@ -27,7 +27,7 @@ workflow QC_WORKFLOW {
         ch_rnaseq_metrics = PICARD_COLLECTRNASEQMETRICS.out.metrics
 
         GATK4_MARKDUPLICATES(
-            ch_bam_sorted.map { meta, bam, _bai -> [ meta, bam ]},
+            ch_bam_sorted,
             ch_fasta.map { _meta, fasta -> [ fasta ]},
             ch_fai.map { _meta, fasta_fai -> [ fasta_fai ]}
         )
@@ -35,7 +35,7 @@ workflow QC_WORKFLOW {
         ch_duplicate_metrics = GATK4_MARKDUPLICATES.out.metrics
 
         PICARD_COLLECTINSERTSIZEMETRICS(
-            ch_bam_sorted.map { meta, bam, _bai -> [ meta, bam ]}
+            ch_bam_sorted
         )
         ch_versions = ch_versions.mix(PICARD_COLLECTINSERTSIZEMETRICS.out.versions)
         ch_insertsize_metrics = PICARD_COLLECTINSERTSIZEMETRICS.out.metrics
diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index 5fdd96d48..cda5cc1e8 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -9,21 +9,18 @@ workflow STARFUSION_WORKFLOW {
         reads               // channel: [ meta, bam ]
         junctions           // channel: [ meta, junctions ]
         ch_starfusion_ref
+        starfusion_fusions
 
     main:
         def ch_versions = Channel.empty()
-        def ch_align = Channel.empty()
         def ch_starfusion_fusions = Channel.empty()
-        def bam_sorted_indexed = Channel.empty()
 
-
-        if (params.starfusion_fusions){
-            ch_starfusion_fusions = reads.combine(Channel.value(file(params.starfusion_fusions, checkIfExists:true)))
+        if (starfusion_fusions){
+            ch_starfusion_fusions = reads.combine(Channel.value(file(starfusion_fusions, checkIfExists:true)))
                                     .map { it -> [ it[0], it[2] ] }
         } else {
             reads_junction = reads.join(junctions)  // TODO: This join is not needed as STARFUSION can simply read from the junction file: https://github.com/STAR-Fusion/STAR-Fusion/wiki#alternatively-kickstart-mode-running-star-yourself-and-then-running-star-fusion-using-the-existing-outputs
 
-
             STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
             ch_versions = ch_versions.mix(STARFUSION.out.versions)
             ch_starfusion_fusions = STARFUSION.out.fusions
@@ -31,8 +28,6 @@ workflow STARFUSION_WORKFLOW {
         }
     emit:
         fusions               = ch_starfusion_fusions
-        ch_bam_sorted         = ch_align
-        ch_bam_sorted_indexed = bam_sorted_indexed
         versions              = ch_versions
     }
 
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
index ac0e77982..af6b117ff 100644
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test
+++ b/subworkflows/local/starfusion_workflow/tests/main.nf.test
@@ -99,10 +99,6 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.fusions,
-                    file(workflow.out.star_stats[0][1]).name,
-                    workflow.out.star_gene_count,
-                    workflow.out.ch_bam_sorted,
-                    workflow.out.ch_bam_sorted_indexed,
                     workflow.out.versions
                 ).match() }
             )
diff --git a/subworkflows/local/stringtie_workflow/main.nf b/subworkflows/local/stringtie_workflow/main.nf
index d9e65fd11..28fa0547e 100644
--- a/subworkflows/local/stringtie_workflow/main.nf
+++ b/subworkflows/local/stringtie_workflow/main.nf
@@ -12,7 +12,7 @@ workflow STRINGTIE_WORKFLOW {
         ch_stringtie_gtf = Channel.empty()
 
         STRINGTIE_STRINGTIE(
-            bam_sorted.map { meta, bam, _bai -> [ meta, bam ]},
+            bam_sorted,
             ch_chrgtf.map { _meta, gtf -> [ gtf ]}
         )
         ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 1cc525704..bc95ad6fc 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -25,9 +25,6 @@ include { paramsSummaryMultiqc          }   from '../subworkflows/nf-core/utils_
 include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
-include { ARRIBA_ARRIBA } from '../modules/nf-core/arriba/arriba/main.nf'
-include { FUSIONREPORT } from '../modules/local/fusionreport/detect/main.nf'
-
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -108,13 +105,13 @@ workflow RNAFUSION {
         def ch_aligned_reads = Channel.empty()
         def ch_star_junctions = Channel.empty()
         def ch_star_split_junctions = Channel.empty()
-        if((params.arriba || params.ctatsplicing || params.starfusion || params.all) && !params.fusioninspector_only) {
+        if(tools.intersect(["ctatsplicing", "arriba", "starfusion", "stringtie"])) {
             FASTQ_ALIGN_STAR(
                 ch_reads,
-                BUILD_REFERENCES.out.ch_starindex_ref,
-                BUILD_REFERENCES.out.ch_gtf,
-                BUILD_REFERENCES.out.ch_fasta,
-                BUILD_REFERENCES.out.ch_fai,
+                BUILD_REFERENCES.out.starindex_ref,
+                BUILD_REFERENCES.out.gtf,
+                BUILD_REFERENCES.out.fasta,
+                BUILD_REFERENCES.out.fai,
                 params.star_ignore_sjdbgtf,
                 params.seq_platform,
                 params.seq_center,
@@ -132,12 +129,12 @@ workflow RNAFUSION {
         // Run CTAT-SPLICING
         //
 
-        if((params.ctatsplicing || params.all)) {
+        if((tools.contains("ctatsplicing"))) {
             CTATSPLICING_WORKFLOW(
                 ch_star_split_junctions,
                 ch_star_junctions,
-                ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
-                BUILD_REFERENCES.out.ch_starfusion_ref
+                ch_aligned_reads,
+                BUILD_REFERENCES.out.starfusion_ref
             )
             ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
         }
@@ -157,13 +154,11 @@ workflow RNAFUSION {
                 ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
                 BUILD_REFERENCES.out.gtf,
                 BUILD_REFERENCES.out.fasta,
-                BUILD_REFERENCES.out.starindex_ref,
                 BUILD_REFERENCES.out.arriba_ref_blacklist,
                 BUILD_REFERENCES.out.arriba_ref_cytobands,
                 BUILD_REFERENCES.out.arriba_ref_known_fusions,
                 BUILD_REFERENCES.out.arriba_ref_protein_domains,
-                params.arriba_fusions,           // path
-                params.cram                      // array
+                params.arriba_fusions
             )
             ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
             ch_arriba_fusions = ARRIBA_WORKFLOW.out.fusions
@@ -174,19 +169,16 @@ workflow RNAFUSION {
         //
 
         def ch_starfusion_fusions = ch_reads.map { it -> [it[0], []] } // Set starfusion fusions to empty by default
-        def ch_starfusion_bams = Channel.empty()
-        if(tools.intersect(["starfusion", "ctatsplicing", "stringtie"])) {
+        if(tools.contains("starfusion")) {
             fusions_created = true
             STARFUSION_WORKFLOW (
                 ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
                 ch_star_junctions,
-                BUILD_REFERENCES.out.starfusion_ref
+                BUILD_REFERENCES.out.starfusion_ref,
+                params.starfusion_fusions
             )
             ch_versions             = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
             ch_starfusion_fusions   = STARFUSION_WORKFLOW.out.fusions
-            ch_starfusion_bams      = STARFUSION_WORKFLOW.out.ch_bam_sorted_indexed
-            ch_multiqc_files        = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_stats.collect{it[1]})
-            ch_multiqc_files        = ch_multiqc_files.mix(STARFUSION_WORKFLOW.out.star_gene_count.collect{it[1]})
         }
 
         //
@@ -261,7 +253,7 @@ workflow RNAFUSION {
                 ch_fusion_list_filtered,
                 ch_fusionreport_report,
                 ch_fusionreport_csv,
-                ch_starfusion_bams,
+                ch_aligned_reads,
                 BUILD_REFERENCES.out.gtf,
                 BUILD_REFERENCES.out.arriba_ref_protein_domains,
                 BUILD_REFERENCES.out.arriba_ref_cytobands,

From 1b815421018e5ace5d46914be73082e7649ac9ad Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 3 Apr 2025 17:05:52 +0200
Subject: [PATCH 407/887] fix last couple of issues integrated with the dev
 merge

---
 subworkflows/local/build_references.nf |    4 +-
 tests/test_stub.nf.test.snap           | 1409 +++++++++++++++++-------
 workflows/rnafusion.nf                 |    2 +-
 3 files changed, 1012 insertions(+), 403 deletions(-)

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index b50e04d3f..8612b8f84 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -165,9 +165,7 @@ workflow BUILD_REFERENCES {
     }
 
     def ch_starfusion_ref = Channel.empty()
-    //TODO update list once alignment is done in a single step
-    def starfusion_tools = tools.intersect(["starfusion", "ctatsplicing", "fusioninspector", "stringtie"])
-    if (starfusion_tools) {
+    if (tools.intersect(["starfusion", "ctatsplicing"])) {
         if (!exists_not_empty(params.starfusion_ref)) {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 7895e56e3..a94c79609 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,18 +1,8 @@
 {
     "stub test starfusion": {
         "content": [
-<<<<<<<<< Temporary merge branch 1
-            29,
-=========
-            32,
->>>>>>>>> Temporary merge branch 2
+            16,
             {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -22,6 +12,10 @@
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
                 "GENCODE_DOWNLOAD": {
                     "wget": null
                 },
@@ -31,10 +25,25 @@
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "STAR_FOR_ARRIBA": {
+                "SAMTOOLS_INDEX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
                     "gawk": "5.1.0"
@@ -49,9 +58,6 @@
                 }
             },
             [
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -64,14 +70,18 @@
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -95,60 +105,130 @@
                 "references/star/sjdbList.fromGTF.out.tab",
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam"
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-02T13:24:01.289397616"
+        "timestamp": "2025-04-03T15:41:41.407790326"
     },
-    "stub test fusioninspector": {
+    "stub test stringtie": {
         "content": [
-            17,
+            16,
             {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
-                },
-                "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
-                },
-                "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
-                },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
                 "GENCODE_DOWNLOAD": {
                     "wget": null
                 },
@@ -158,16 +238,19 @@
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
-                "HGNC_DOWNLOAD": {
-                    "wget": null
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
                 },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "STARFUSION_BUILD": {
-                    "STAR-Fusion": "1.14.0"
+                "SAMTOOLS_INDEX": {
+                    "samtools": 1.21
                 },
-                "STAR_FOR_ARRIBA": {
+                "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
                     "gawk": "5.1.0"
@@ -177,136 +260,815 @@
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
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-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
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-                "fusioninspector/IGV_inputs",
-                "fusioninspector/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/IGV_inputs/cytoBand.txt",
-                "fusioninspector/IGV_inputs/test.ROI.bed",
-                "fusioninspector/IGV_inputs/test.bed",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/IGV_inputs/test.fa",
-                "fusioninspector/IGV_inputs/test.fa.fai",
-                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/IGV_inputs/test.gtf",
-                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/IGV_inputs/tracks.json",
-                "fusioninspector/chckpts_dir",
-                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/chckpts_dir/blast_filter.ok",
-                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/chckpts_dir/cp_final.ok",
-                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/chckpts_dir/cytoband.ok",
-                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/chckpts_dir/final.abridged.ok",
-                "fusioninspector/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/chckpts_dir/microH.dat.ok",
-                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/chckpts_dir/run_STAR.ok",
-                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/fi_workdir",
-                "fusioninspector/fi_workdir/Log.final.out",
-                "fusioninspector/fi_workdir/Log.out",
-                "fusioninspector/fi_workdir/Log.progress.out",
-                "fusioninspector/fi_workdir/SJ.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome",
-                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/fi_workdir/microH.dat",
-                "fusioninspector/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/fi_workdir/star_align.ok",
-                "fusioninspector/fi_workdir/test.fa",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/fi_workdir/test.gtf",
-                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.fusion_inspector_web.html",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-03T15:50:39.921183013"
+    },
+    "stub test arriba": {
+        "content": [
+            16,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
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+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX": {
+                    "samtools": 1.21
+                },
+                "STAR_ALIGN": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-03T15:39:53.022836825"
+    },
+    "stub test fusioninspector": {
+        "content": [
+            19,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
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+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
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+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
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+                },
+                "GET_RRNA_TRANSCRIPTS": {
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+                },
+                "HGNC_DOWNLOAD": {
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+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
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+                    "gawk": "5.1.0"
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+                    "fusion_report": "2.1.5"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_ALIGN": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fusioncatcher",
+                "fusioncatcher/human_v46",
+                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
+                "fusioncatcher/test.fusioncatcher.log",
+                "fusioncatcher/test.fusioncatcher.summary.txt",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -324,6 +1086,15 @@
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
@@ -344,9 +1115,6 @@
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
                 "references/star",
                 "references/star/Genome",
                 "references/star/Log.out",
@@ -440,39 +1208,50 @@
                 "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
                 "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
                 "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam"
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-02T13:47:39.929302498"
+        "timestamp": "2025-04-03T15:43:02.916057311"
     },
-    "stub test salmon": {
+    "stub test fastp": {
         "content": [
-            11,
+            10,
             {
+                "FASTP": {
+                    "fastp": "0.23.4"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
                 },
@@ -485,18 +1264,9 @@
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
                 },
-                "GFFREAD": {
-                    "gffread": "0.12.7"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
-                "SALMON_INDEX": {
-                    "salmon": "1.10.3"
-                },
-                "SALMON_QUANT": {
-                    "salmon": "1.10.3"
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
@@ -505,9 +1275,18 @@
                 }
             },
             [
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "get",
@@ -525,61 +1304,34 @@
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
-                "salmon",
-                "salmon/test",
-                "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json"
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-02T13:32:18.882484433"
+        "timestamp": "2025-04-03T15:43:33.740487484"
     },
-    "stub test ctatsplicing": {
+    "stub test fusioncatcher": {
         "content": [
-            19,
+            10,
             {
-                "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "CTATSPLICING_STARTOCANCERINTRONS": {
-                    "ctat-splicing": "0.0.2"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
+                "FUSIONCATCHER_BUILD": {
+                    "fusioncatcher": "1.33"
+                },
+                "FUSIONCATCHER_DETECT": {
+                    "fusioncatcher": "1.33"
+                },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
-                },
                 "GENCODE_DOWNLOAD": {
                     "wget": null
                 },
@@ -589,61 +1341,14 @@
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "SAMTOOLS_INDEX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION_BUILD": {
-                    "STAR-Fusion": "1.14.0"
-                },
-                "STAR_ALIGN": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.2,
-                    "gawk": "5.1.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "ctatsplicing",
-                "ctatsplicing/arriba",
-                "ctatsplicing/arriba/test.cancer.introns",
-                "ctatsplicing/arriba/test.cancer.introns.prelim",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/arriba/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/arriba/test.chckpts",
-                "ctatsplicing/arriba/test.ctat-splicing.igv.html",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/arriba/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/arriba/test.igv.tracks",
-                "ctatsplicing/arriba/test.introns",
-                "ctatsplicing/arriba/test.introns.for_IGV.bed",
-                "ctatsplicing/starfusion",
-                "ctatsplicing/starfusion/test.cancer.introns",
-                "ctatsplicing/starfusion/test.cancer.introns.prelim",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/starfusion/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/starfusion/test.chckpts",
-                "ctatsplicing/starfusion/test.ctat-splicing.igv.html",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/starfusion/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/starfusion/test.igv.tracks",
-                "ctatsplicing/starfusion/test.introns",
-                "ctatsplicing/starfusion/test.introns.for_IGV.bed",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -653,14 +1358,6 @@
                 "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
                 "fusioncatcher/test.fusioncatcher.log",
                 "fusioncatcher/test.fusioncatcher.summary.txt",
-                "fusionreport",
-                "fusionreport/test",
-                "fusionreport/test/AAA_BBB.html",
-                "fusionreport/test/test.fusionreport.tsv",
-                "fusionreport/test/test.fusionreport_filtered.tsv",
-                "fusionreport/test/test.fusions.csv",
-                "fusionreport/test/test.fusions.json",
-                "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "get",
@@ -670,112 +1367,26 @@
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
                 "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "salmon",
-                "salmon/test",
-                "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
-                "star_for_arriba",
-                "star_for_arriba/test.Aligned.sortedByCoord.out.bam",
-                "star_for_arriba/test.Aligned.unsort.out.bam",
-                "star_for_arriba/test.Chimeric.out.junction",
-                "star_for_arriba/test.Log.final.out",
-                "star_for_arriba/test.Log.out",
-                "star_for_arriba/test.Log.progress.out",
-                "star_for_arriba/test.ReadsPerGene.out.tab",
-                "star_for_arriba/test.SJ.out.tab",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_arriba/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_arriba/test.out.sam",
-                "star_for_arriba/test.sortedByCoord.out.bam",
-                "star_for_arriba/test.tab",
-                "star_for_arriba/test.toTranscriptome.out.bam",
-                "star_for_arriba/test.unmapped_1.fastq.gz",
-                "star_for_arriba/test.unmapped_2.fastq.gz",
-                "star_for_arriba/testXd.out.bam",
-                "star_for_starfusion",
-                "star_for_starfusion/test.Aligned.sortedByCoord.out.bam",
-                "star_for_starfusion/test.Aligned.unsort.out.bam",
-                "star_for_starfusion/test.Chimeric.out.junction",
-                "star_for_starfusion/test.Log.final.out",
-                "star_for_starfusion/test.Log.out",
-                "star_for_starfusion/test.Log.progress.out",
-                "star_for_starfusion/test.ReadsPerGene.out.tab",
-                "star_for_starfusion/test.SJ.out.tab",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.bg",
-                "star_for_starfusion/test.Signal.UniqueMultiple.str1.out.wig",
-                "star_for_starfusion/test.out.sam",
-                "star_for_starfusion/test.sortedByCoord.out.bam",
-                "star_for_starfusion/test.sortedByCoord.out.bam.bai",
-                "star_for_starfusion/test.tab",
-                "star_for_starfusion/test.toTranscriptome.out.bam",
-                "star_for_starfusion/test.unmapped_1.fastq.gz",
-                "star_for_starfusion/test.unmapped_2.fastq.gz",
-                "star_for_starfusion/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv"
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-17T17:40:40.562156411"
+        "timestamp": "2025-04-03T15:40:58.774714515"
     },
-    "stub test fastp": {
+    "stub test all": {
         "content": [
-<<<<<<<<< Temporary merge branch 1
-            31,
-=========
             34,
->>>>>>>>> Temporary merge branch 2
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1246,6 +1857,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-17T17:38:45.56378377"
+        "timestamp": "2025-04-03T15:39:16.250051213"
     }
-}
+}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bc95ad6fc..dbe490d5a 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -129,7 +129,7 @@ workflow RNAFUSION {
         // Run CTAT-SPLICING
         //
 
-        if((tools.contains("ctatsplicing"))) {
+        if(tools.contains("ctatsplicing")) {
             CTATSPLICING_WORKFLOW(
                 ch_star_split_junctions,
                 ch_star_junctions,

From 776fa880de8210fe280dd382ba3ce808d55e9e46 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 7 Apr 2025 15:33:22 +0200
Subject: [PATCH 408/887] remove unused includes in starfusion_workflow

---
 subworkflows/local/starfusion_workflow/main.nf | 4 ----
 1 file changed, 4 deletions(-)

diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index cda5cc1e8..c4fa164c5 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -1,8 +1,4 @@
-include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION       }   from '../../../modules/nf-core/samtools/index/main'
-include { SAMTOOLS_INDEX as SAMTOOLS_INDEX_FOR_STARFUSION_CRAM  }   from '../../../modules/nf-core/samtools/index/main'
-include { SAMTOOLS_VIEW as SAMTOOLS_VIEW_FOR_STARFUSION         }   from '../../../modules/nf-core/samtools/view/main'
 include { STARFUSION                                            }   from '../../../modules/local/starfusion/detect/main'
-include { CTATSPLICING_WORKFLOW                                 }   from '../ctatsplicing_workflow'
 
 workflow STARFUSION_WORKFLOW {
     take:

From 472a4f290ee624218855d32425d42056574a1b44 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 8 Apr 2025 11:05:18 +0200
Subject: [PATCH 409/887] correct starfusion input

---
 subworkflows/local/starfusion_workflow/main.nf | 13 ++++++-------
 workflows/rnafusion.nf                         |  1 -
 2 files changed, 6 insertions(+), 8 deletions(-)

diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index c4fa164c5..53f0dd8a4 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -2,7 +2,6 @@ include { STARFUSION                                            }   from '../../
 
 workflow STARFUSION_WORKFLOW {
     take:
-        reads               // channel: [ meta, bam ]
         junctions           // channel: [ meta, junctions ]
         ch_starfusion_ref
         starfusion_fusions
@@ -12,15 +11,15 @@ workflow STARFUSION_WORKFLOW {
         def ch_starfusion_fusions = Channel.empty()
 
         if (starfusion_fusions){
-            ch_starfusion_fusions = reads.combine(Channel.value(file(starfusion_fusions, checkIfExists:true)))
-                                    .map { it -> [ it[0], it[2] ] }
+            fusions = file(starfusion_fusions, checkIfExists:true)
+            ch_starfusion_fusions = junctions.map { meta, _junc -> [ meta, fusions ] }
         } else {
-            reads_junction = reads.join(junctions)  // TODO: This join is not needed as STARFUSION can simply read from the junction file: https://github.com/STAR-Fusion/STAR-Fusion/wiki#alternatively-kickstart-mode-running-star-yourself-and-then-running-star-fusion-using-the-existing-outputs
-
-            STARFUSION( reads_junction, ch_starfusion_ref.map { it -> it[1] })
+            STARFUSION(
+                junctions.map { meta, junc -> [ meta, [], junc ]},
+                ch_starfusion_ref.map { it -> it[1] }
+            )
             ch_versions = ch_versions.mix(STARFUSION.out.versions)
             ch_starfusion_fusions = STARFUSION.out.fusions
-
         }
     emit:
         fusions               = ch_starfusion_fusions
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index dbe490d5a..69d317fb6 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -172,7 +172,6 @@ workflow RNAFUSION {
         if(tools.contains("starfusion")) {
             fusions_created = true
             STARFUSION_WORKFLOW (
-                ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
                 ch_star_junctions,
                 BUILD_REFERENCES.out.starfusion_ref,
                 params.starfusion_fusions

From 1ec0fb6e9879e3944851eac54ae874282e7c01d7 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 8 Apr 2025 13:52:16 +0200
Subject: [PATCH 410/887] add support for multiple input types in the
 samplesheet

---
 assets/schema_input.json                      | 63 +++++++++++++-
 modules/local/fusionreport/detect/main.nf     |  2 +-
 .../local/ctatsplicing_workflow/main.nf       |  6 +-
 .../local/fusionreport_workflow/main.nf       |  6 +-
 .../utils_nfcore_rnafusion_pipeline/main.nf   | 45 +++++++---
 workflows/rnafusion.nf                        | 83 +++++++++++++++----
 6 files changed, 166 insertions(+), 39 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index f38d29c48..ad1c753cc 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -27,12 +27,73 @@
                 "pattern": "^\\S+f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 2 cannot contain spaces, has to exist and must have extension '.fq.gz' or '.fastq.gz'"
             },
+            "bam": {
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.bam$",
+                "errorMessage": "BAM file cannot contain spaces, has to exist and must have extension '.bam'"
+            },
+            "bai": {
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.bam\\.bai$",
+                "errorMessage": "BAI file cannot contain spaces, has to exist and must have extension '.bam.bai'"
+            },
+            "cram": {
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.cram$",
+                "errorMessage": "CRAM file cannot contain spaces, has to exist and must have extension '.cram'"
+            },
+            "crai": {
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.cram\\.crai$",
+                "errorMessage": "CRAI file cannot contain spaces, has to exist and must have extension '.cram.crai'"
+            },
+            "junctions": {
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.junction$",
+                "errorMessage": "Junctions file cannot contain spaces, has to exist and must have extension '.junction'"
+            },
+            "split_junctions": {
+                "type": "string",
+                "format": "file-path",
+                "exists": true,
+                "pattern": "^\\S+\\.SJ.out.tab$",
+                "errorMessage": "Split junctions file cannot contain spaces, has to exist and must have extension '.SJ.out.tab'"
+            },
             "strandedness": {
                 "type": "string",
                 "enum": ["forward", "reverse", "unstranded", "unknown"],
                 "errorMessage": "Strandedness has to be 'forward', 'reverse', 'unstranded' or 'unknown'"
             }
         },
-        "required": ["sample", "fastq_1", "fastq_2", "strandedness"]
+        "dependentRequired": {
+            "fastq_2": ["fastq_1"],
+            "bai": ["bam"],
+            "crai": ["cram"]
+        },
+        "required": ["sample", "strandedness"],
+        "oneOf": [
+            {
+                "required": ["fastq_1", "fastq_2"]
+            },
+            {
+                "required": ["bam"]
+            },
+            {
+                "required": ["cram"]
+            },
+            {
+                "required": ["junctions"]
+            }
+        ]
     }
 }
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index dab70ffe4..bcb59877c 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -9,7 +9,7 @@ process FUSIONREPORT {
 
 
     input:
-    tuple val(meta), path(reads), path(arriba_fusions), path(starfusion_fusions),  path(fusioncatcher_fusions)
+    tuple val(meta), path(arriba_fusions), path(starfusion_fusions),  path(fusioncatcher_fusions)
     tuple val(meta2), path(fusionreport_ref)
     val(tools_cutoff)
 
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
index 7f83802e0..f464e8b66 100644
--- a/subworkflows/local/ctatsplicing_workflow/main.nf
+++ b/subworkflows/local/ctatsplicing_workflow/main.nf
@@ -10,9 +10,9 @@ workflow CTATSPLICING_WORKFLOW {
     main:
     def ch_versions = Channel.empty()
 
-    def ch_ctatsplicing_input = split_junctions
-        .join(junctions, failOnMismatch:true, failOnDuplicate:true)
-        .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
+    def ch_ctatsplicing_input = split_junctions.view()
+        .join(junctions.view(), failOnMismatch:true, failOnDuplicate:true)
+        .join(aligned_bams.view(), failOnMismatch:true, failOnDuplicate:true)
         .map { meta, split_junction, junction, bam, bai ->
             [ meta, split_junction, junction, bam, bai ]
         }
diff --git a/subworkflows/local/fusionreport_workflow/main.nf b/subworkflows/local/fusionreport_workflow/main.nf
index bbbc909fb..c4a896dd7 100644
--- a/subworkflows/local/fusionreport_workflow/main.nf
+++ b/subworkflows/local/fusionreport_workflow/main.nf
@@ -3,7 +3,6 @@ include { FUSIONREPORT      }     from '../../../modules/local/fusionreport/dete
 
 workflow FUSIONREPORT_WORKFLOW {
     take:
-        reads
         fusionreport_ref
         arriba_fusions
         starfusion_fusions
@@ -14,12 +13,11 @@ workflow FUSIONREPORT_WORKFLOW {
         ch_report = Channel.empty()
         ch_csv = Channel.empty()
 
-        reads_fusions = reads
-            .join(arriba_fusions, failOnMismatch:true, failOnDuplicate:true)
+        def ch_fusions = arriba_fusions
             .join(starfusion_fusions, failOnMismatch:true, failOnDuplicate:true)
             .join(fusioncatcher_fusions, failOnMismatch:true, failOnDuplicate:true)
 
-        FUSIONREPORT(reads_fusions, fusionreport_ref, params.tools_cutoff)
+        FUSIONREPORT(ch_fusions, fusionreport_ref, params.tools_cutoff)
         ch_fusion_list = FUSIONREPORT.out.fusion_list
         ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered
         ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index e751b6430..295a23722 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -72,21 +72,24 @@ workflow PIPELINE_INITIALISATION {
 
     Channel
         .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json"))
-        .map {
-            meta, fastq_1, fastq_2, strandedness ->
-                if (!fastq_2) {
-                    return [ meta.id, meta + [ single_end:true ], [ fastq_1 ], strandedness ]
-                } else {
-                    return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ], strandedness ]
-                }
+        .map { meta, fastq_1, fastq_2, bam, bai, cram, crai, junctions, split_junctions, strandedness ->
+            def meta_fastqs = []
+            if (!fastq_1) {
+                meta_fastqs = [ meta, [] ]
+            } else if (!fastq_2) {
+                meta_fastqs = [ meta + [single_end:true], [ fastq_1 ] ]
+            } else {
+                meta_fastqs = [ meta + [single_end:false], [ fastq_1, fastq_2 ] ]
+            }
+            return [ meta.id ] + meta_fastqs + [ bam, bai, cram, crai, junctions, split_junctions, strandedness ]
         }
         .groupTuple()
         .map { samplesheet ->
             validateInputSamplesheet(samplesheet)
         }
         .map {
-            meta, fastqs ->
-                return [ meta, fastqs.flatten() ]
+            meta, fastqs, bam, bai, cram, crai, junctions, split_junctions ->
+                return [ meta, fastqs.flatten(), bam, bai, cram, crai, junctions, split_junctions ]
         }
         .set { ch_samplesheet }
 
@@ -165,7 +168,25 @@ def validateInputParameters() {
 // Validate channels from input samplesheet
 //
 def validateInputSamplesheet(input) {
-    def (metas, fastqs) = input[1..2]
+    def (metas, fastqs, bam, bai, cram, crai, junctions, split_junctions) = input[1..8]
+
+    def bam_list = bam.findAll { it -> it != [] }
+    def cram_list = cram.findAll { it -> it != [] }
+    def junctions_list = bai.findAll { it -> it != [] }
+    def split_junctions_list = split_junctions.findAll { it -> it != [] }
+    // Check alignment and junction files (input is a list)
+    if (bam_list.size() > 1 || cram_list.size() > 1 || junctions_list.size() > 1 || split_junctions_list.size() > 1) {
+        error("Please check input samplesheet -> Only one BAM or CRAM, junctions and split junctions file is allowed per sample: ${metas[0].id}")
+    }
+
+    bam = bam_list.size() > 0 ? bam_list[0] : []
+    cram = cram_list.size() > 0 ? cram_list[0] : []
+    junctions = junctions_list.size() > 0 ? junctions_list[0] : []
+    split_junctions = split_junctions_list.size() > 0 ? split_junctions_list[0] : []
+
+    if (bam != [] && cram != []) {
+        error("Please check input samplesheet -> Using both BAM and CRAM files isn't allowed: ${metas[0].id}")
+    }
 
     // Check that multiple runs of the same sample are of the same strandedness
     def strandedness_ok = metas.collect{ it.strandedness }.unique().size == 1
@@ -175,11 +196,11 @@ def validateInputSamplesheet(input) {
 
     // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end
     def endedness_ok = metas.collect{ meta -> meta.single_end }.unique().size == 1
-    if (!endedness_ok) {
+    if (!endedness_ok && fastqs) {
         error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}")
     }
 
-    return [ metas[0], fastqs ]
+    return [ metas[0], fastqs, bam, bai.find { it -> it != [] } ?: [], cram, crai.find { it -> it != [] } ?: [], junctions, split_junctions ]
 }
 
 //
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 69d317fb6..1e62d4a77 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -53,13 +53,52 @@ workflow RNAFUSION {
 
     if (!params.references_only) {
 
+        def ch_input = ch_samplesheet.multiMap { meta, fastqs, bam, bai, cram, crai, junctions, split_junctions ->
+            def align = false
+                // Check if we need split junctions
+                if (tools.contains("ctatsplicing") && !split_junctions) {
+                    align = true
+                }
+
+                // Check if we need junctions
+                if (tools.intersect(["starfusion", "ctatsplicing"]) && !junctions) {
+                    align = true
+                }
+
+                // Check if we need BAM or CRAM files
+                if (tools.intersect(["ctatsplicing", "arriba", "stringtie"]) && !bam && !cram) {
+                    align = true
+                }
+
+                // Check if there are fastqs when we need to align
+                if (align && !fastqs) {
+                    error("No fastq files found for ${meta.id}. Either provide fastq files to align or provide a BAM/CRAM file and a junctions file.")
+                }
+            def new_meta = meta + [align:align]
+            fastqs:             [ new_meta, fastqs ]
+            bam:                [ new_meta, bam, bai ]
+            cram:               [ new_meta, cram, crai ]
+            junctions:          [ new_meta, junctions ]
+            split_junctions:    [ new_meta, split_junctions ]
+        }
+
+        // Define which fastqs need to be processes (all analysis that's not aligning)
+        def fastq_tools = ["salmon", "fusioninspector"]
+        selected_fastq_tools = tools.intersect(fastq_tools)
+        def ch_fastqs_to_process = ch_input.fastqs.filter { meta, fastqs ->
+            if (!fastqs && selected_fastq_tools) {
+                log.warn("Fastq files not found for ${meta.id}. Skipping the following tools for this sample: ${selected_fastq_tools.join(', ')}")
+            }
+            return fastqs
+        }
+
         //
         // QC from FASTQ files
         //
 
         if(!params.skip_qc) {
             FASTQC (
-                ch_samplesheet
+                ch_fastqs_to_process,
             )
             ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
             ch_versions = ch_versions.mix(FASTQC.out.versions)
@@ -73,7 +112,7 @@ workflow RNAFUSION {
         if(tools.contains("fastp")) {
             def ch_adapter_fasta = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta).collect() : []
             TRIM_WORKFLOW (
-                ch_samplesheet,
+                ch_fastqs_to_process,
                 ch_adapter_fasta,
                 params.skip_qc
             )
@@ -83,9 +122,13 @@ workflow RNAFUSION {
             ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]})
             ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]})
         } else {
-            ch_reads = ch_samplesheet
+            ch_reads = ch_fastqs_to_process
         }
 
+        //
+        // MODULE: SALMON_QUANT
+        //
+
         if(tools.contains("salmon") && !params.skip_qc) {
             SALMON_QUANT(
                 ch_reads,
@@ -98,16 +141,22 @@ workflow RNAFUSION {
             ch_multiqc_files = ch_multiqc_files.mix(SALMON_QUANT.out.json_info.collect{it[1]})
             ch_versions      = ch_versions.mix(SALMON_QUANT.out.versions)
         }
+
         //
-        // Run STAR alignment
+        // SUBWORKFLOW: Run STAR alignment
         //
 
-        def ch_aligned_reads = Channel.empty()
-        def ch_star_junctions = Channel.empty()
-        def ch_star_split_junctions = Channel.empty()
+        // Define which fastqs need to be aligned
+        def ch_fastqs_to_align = ch_reads
+            .filter { meta, _fastqs -> meta.align }
+
+        // Add the alignment files to the correct channel if their fastqs aren't aligned
+        def ch_aligned_reads        = ch_input.bam.mix(ch_input.cram).filter { meta, file, _index -> file && !meta.align }
+        def ch_star_junctions       = ch_input.junctions.filter { meta, file -> file && !meta.align }
+        def ch_star_split_junctions = ch_input.split_junctions.filter { meta, file -> file && !meta.align }
         if(tools.intersect(["ctatsplicing", "arriba", "starfusion", "stringtie"])) {
             FASTQ_ALIGN_STAR(
-                ch_reads,
+                ch_fastqs_to_align,
                 BUILD_REFERENCES.out.starindex_ref,
                 BUILD_REFERENCES.out.gtf,
                 BUILD_REFERENCES.out.fasta,
@@ -117,16 +166,16 @@ workflow RNAFUSION {
                 params.seq_center,
                 params.cram
             )
-            ch_versions = ch_versions.mix(FASTQ_ALIGN_STAR.out.versions)
-            ch_aligned_reads = FASTQ_ALIGN_STAR.out.bam_bai
-            ch_star_junctions = FASTQ_ALIGN_STAR.out.junctions
-            ch_star_split_junctions = FASTQ_ALIGN_STAR.out.spl_junc_tabs
-            ch_multiqc_files = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.log_final.collect{it[1]}.ifEmpty([]))
-            ch_multiqc_files = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.gene_count.collect{it[1]}.ifEmpty([]))
+            ch_versions             = ch_versions.mix(FASTQ_ALIGN_STAR.out.versions)
+            ch_aligned_reads        = ch_aligned_reads.mix(FASTQ_ALIGN_STAR.out.bam_bai)
+            ch_star_junctions       = ch_star_junctions.mix(FASTQ_ALIGN_STAR.out.junctions)
+            ch_star_split_junctions = ch_star_split_junctions.mix(FASTQ_ALIGN_STAR.out.spl_junc_tabs)
+            ch_multiqc_files        = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.log_final.collect{it[1]}.ifEmpty([]))
+            ch_multiqc_files        = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.gene_count.collect{it[1]}.ifEmpty([]))
         }
 
         //
-        // Run CTAT-SPLICING
+        // SUBWORKFLOW: Run CTAT-SPLICING
         //
 
         if(tools.contains("ctatsplicing")) {
@@ -140,11 +189,10 @@ workflow RNAFUSION {
         }
 
         //
-        // SUBWORKFLOW: Run STAR alignment and Arriba
+        // SUBWORKFLOW: Run Arriba
         //
 
         // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
-        // TODO: same as above, but with ch_arriba_fusion_fail. It's currently replaces by a dummy file
 
         def fusions_created = false
         def ch_arriba_fusions = ch_reads.map { it -> [it[0], []] } // Set arriba fusions to empty by default
@@ -220,7 +268,6 @@ workflow RNAFUSION {
                 error("Could not find any fusion files. Please generate some with --arriba, --starfusion and/or --fusioncatcher")
             }
             FUSIONREPORT_WORKFLOW (
-                ch_reads,
                 BUILD_REFERENCES.out.fusionreport_ref,
                 ch_arriba_fusions,
                 ch_starfusion_fusions,

From ef67463cf48bab712e1b8caf2e3169435bd92088 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 8 Apr 2025 14:43:59 +0200
Subject: [PATCH 411/887] fix some issues in channel handling

---
 assets/schema_input.json                      |  5 +++-
 .../local/ctatsplicing_workflow/main.nf       |  6 ++---
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  2 +-
 workflows/rnafusion.nf                        | 23 +++++++++++--------
 4 files changed, 21 insertions(+), 15 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index ad1c753cc..c472b893c 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -81,7 +81,7 @@
             "crai": ["cram"]
         },
         "required": ["sample", "strandedness"],
-        "oneOf": [
+        "anyOf": [
             {
                 "required": ["fastq_1", "fastq_2"]
             },
@@ -93,6 +93,9 @@
             },
             {
                 "required": ["junctions"]
+            },
+            {
+                "required": ["split_junctions"]
             }
         ]
     }
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
index f464e8b66..7f83802e0 100644
--- a/subworkflows/local/ctatsplicing_workflow/main.nf
+++ b/subworkflows/local/ctatsplicing_workflow/main.nf
@@ -10,9 +10,9 @@ workflow CTATSPLICING_WORKFLOW {
     main:
     def ch_versions = Channel.empty()
 
-    def ch_ctatsplicing_input = split_junctions.view()
-        .join(junctions.view(), failOnMismatch:true, failOnDuplicate:true)
-        .join(aligned_bams.view(), failOnMismatch:true, failOnDuplicate:true)
+    def ch_ctatsplicing_input = split_junctions
+        .join(junctions, failOnMismatch:true, failOnDuplicate:true)
+        .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
         .map { meta, split_junction, junction, bam, bai ->
             [ meta, split_junction, junction, bam, bai ]
         }
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 295a23722..e5eefe820 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -172,7 +172,7 @@ def validateInputSamplesheet(input) {
 
     def bam_list = bam.findAll { it -> it != [] }
     def cram_list = cram.findAll { it -> it != [] }
-    def junctions_list = bai.findAll { it -> it != [] }
+    def junctions_list = junctions.findAll { it -> it != [] }
     def split_junctions_list = split_junctions.findAll { it -> it != [] }
     // Check alignment and junction files (input is a list)
     if (bam_list.size() > 1 || cram_list.size() > 1 || junctions_list.size() > 1 || split_junctions_list.size() > 1) {
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 1e62d4a77..551c2e389 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -83,13 +83,14 @@ workflow RNAFUSION {
         }
 
         // Define which fastqs need to be processes (all analysis that's not aligning)
-        def fastq_tools = ["salmon", "fusioninspector"]
+        def fastq_tools = ["salmon", "fusioninspector", "fusioncatcher"]
         selected_fastq_tools = tools.intersect(fastq_tools)
-        def ch_fastqs_to_process = ch_input.fastqs.filter { meta, fastqs ->
+        def ch_fastqs_to_process = ch_input.fastqs.branch { meta, fastqs ->
             if (!fastqs && selected_fastq_tools) {
-                log.warn("Fastq files not found for ${meta.id}. Skipping the following tools for this sample: ${selected_fastq_tools.join(', ')}")
+                log.warn("Fastq files not found for sample '${meta.id}'. Skipping the following tools for this sample: ${selected_fastq_tools.join(', ')}")
             }
-            return fastqs
+            found: fastqs
+            not_found: !fastqs
         }
 
         //
@@ -98,7 +99,7 @@ workflow RNAFUSION {
 
         if(!params.skip_qc) {
             FASTQC (
-                ch_fastqs_to_process,
+                ch_fastqs_to_process.found,
             )
             ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
             ch_versions = ch_versions.mix(FASTQC.out.versions)
@@ -112,7 +113,7 @@ workflow RNAFUSION {
         if(tools.contains("fastp")) {
             def ch_adapter_fasta = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta).collect() : []
             TRIM_WORKFLOW (
-                ch_fastqs_to_process,
+                ch_fastqs_to_process.found,
                 ch_adapter_fasta,
                 params.skip_qc
             )
@@ -122,7 +123,7 @@ workflow RNAFUSION {
             ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]})
             ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]})
         } else {
-            ch_reads = ch_fastqs_to_process
+            ch_reads = ch_fastqs_to_process.found
         }
 
         //
@@ -195,7 +196,7 @@ workflow RNAFUSION {
         // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
 
         def fusions_created = false
-        def ch_arriba_fusions = ch_reads.map { it -> [it[0], []] } // Set arriba fusions to empty by default
+        def ch_arriba_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set arriba fusions to empty by default
         if(tools.contains("arriba")) {
             fusions_created = true
             ARRIBA_WORKFLOW (
@@ -216,7 +217,7 @@ workflow RNAFUSION {
         // SUBWORKFLOW: Run STAR alignment and StarFusion
         //
 
-        def ch_starfusion_fusions = ch_reads.map { it -> [it[0], []] } // Set starfusion fusions to empty by default
+        def ch_starfusion_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set starfusion fusions to empty by default
         if(tools.contains("starfusion")) {
             fusions_created = true
             STARFUSION_WORKFLOW (
@@ -232,7 +233,7 @@ workflow RNAFUSION {
         // SUBWORKFLOW: Run FusionCatcher
         //
 
-        def ch_fusioncatcher_fusions = ch_reads.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
+        def ch_fusioncatcher_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
             fusions_created = true
             FUSIONCATCHER_WORKFLOW (
@@ -241,6 +242,8 @@ workflow RNAFUSION {
                 params.fusioncatcher_fusions
             )
             ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
+            // Add output of fusioncatcher to a channel + add empty entries for the samples that could not be run
+            ch_fusioncatcher_fusions = FUSIONCATCHER_WORKFLOW.out.fusions.mix(ch_fastqs_to_process.not_found)
         }
 
         //

From e6495ba25187d63fa4fd39c6b8de8275160d246a Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 8 Apr 2025 15:09:36 +0200
Subject: [PATCH 412/887] add cram -> bam conversion

---
 workflows/rnafusion.nf | 19 ++++++++++++++++++-
 1 file changed, 18 insertions(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 551c2e389..b05851f39 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -18,6 +18,7 @@ include { FASTQC                        }   from '../modules/nf-core/fastqc/main
 include { MULTIQC                       }   from '../modules/nf-core/multiqc/main'
 include { STAR_ALIGN                    }   from '../modules/nf-core/star/align/main'
 include { SALMON_QUANT                  }   from '../modules/nf-core/salmon/quant/main'
+include { SAMTOOLS_CONVERT              }   from '../modules/nf-core/samtools/convert/main'
 include { paramsSummaryMap              }   from 'plugin/nf-schema'
 include { FASTQ_ALIGN_STAR              }   from '../subworkflows/local/fastq_align_star'
 include { CTATSPLICING_WORKFLOW         }   from '../subworkflows/local/ctatsplicing_workflow'
@@ -93,6 +94,22 @@ workflow RNAFUSION {
             not_found: !fastqs
         }
 
+        // Convert CRAM to BAM when needed (when tools that don't support CRAM are used and when the sample isn't aligned)
+        def only_bam_tools = ["ctatsplicing", "stringtie", "fusioninspector"]
+        def ch_aligned_inputs = ch_input.bam.filter { meta, file, _bai -> file && !meta.align }
+        if(tools.intersect(only_bam_tools)) {
+            SAMTOOLS_CONVERT(
+                ch_input.cram.filter { meta, file, _crai -> file && !meta.align },
+                BUILD_REFERENCES.out.fasta,
+                BUILD_REFERENCES.out.fai
+            )
+            ch_aligned_inputs = ch_aligned_inputs.mix(
+                SAMTOOLS_CONVERT.out.bam.join(SAMTOOLS_CONVERT.out.bai, failOnMismatch:true, failOnDuplicate:true)
+            )
+        } else {
+            ch_aligned_inputs = ch_aligned_inputs.mix(ch_input.cram.filter { meta, file, _crai -> file && !meta.align })
+        }
+
         //
         // QC from FASTQ files
         //
@@ -152,7 +169,7 @@ workflow RNAFUSION {
             .filter { meta, _fastqs -> meta.align }
 
         // Add the alignment files to the correct channel if their fastqs aren't aligned
-        def ch_aligned_reads        = ch_input.bam.mix(ch_input.cram).filter { meta, file, _index -> file && !meta.align }
+        def ch_aligned_reads        = ch_aligned_inputs
         def ch_star_junctions       = ch_input.junctions.filter { meta, file -> file && !meta.align }
         def ch_star_split_junctions = ch_input.split_junctions.filter { meta, file -> file && !meta.align }
         if(tools.intersect(["ctatsplicing", "arriba", "starfusion", "stringtie"])) {

From d8d8aaaedf86e65b20c33f3507f210a4fe4636ff Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 8 Apr 2025 15:43:52 +0200
Subject: [PATCH 413/887] improve error message

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index b05851f39..4bb34532e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -73,7 +73,7 @@ workflow RNAFUSION {
 
                 // Check if there are fastqs when we need to align
                 if (align && !fastqs) {
-                    error("No fastq files found for ${meta.id}. Either provide fastq files to align or provide a BAM/CRAM file and a junctions file.")
+                    error("No fastq files found for ${meta.id}. Either provide fastq files to align or provide a BAM/CRAM file, a junctions file and a split junctions file.")
                 }
             def new_meta = meta + [align:align]
             fastqs:             [ new_meta, fastqs ]

From 44d0c8c2620c48c8fab828b1e351f69547809776 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 8 Apr 2025 16:06:15 +0200
Subject: [PATCH 414/887] update docs

---
 assets/schema_input.json                      |  6 ++--
 docs/usage.md                                 | 30 ++++++++++++-------
 .../local/ctatsplicing_workflow/main.nf       |  4 +--
 .../utils_nfcore_rnafusion_pipeline/main.nf   | 18 +++++------
 workflows/rnafusion.nf                        | 14 ++++-----
 5 files changed, 41 insertions(+), 31 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index c472b893c..573619ce9 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -62,7 +62,7 @@
                 "pattern": "^\\S+\\.junction$",
                 "errorMessage": "Junctions file cannot contain spaces, has to exist and must have extension '.junction'"
             },
-            "split_junctions": {
+            "splice_junctions": {
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
@@ -83,7 +83,7 @@
         "required": ["sample", "strandedness"],
         "anyOf": [
             {
-                "required": ["fastq_1", "fastq_2"]
+                "required": ["fastq_1"]
             },
             {
                 "required": ["bam"]
@@ -95,7 +95,7 @@
                 "required": ["junctions"]
             },
             {
-                "required": ["split_junctions"]
+                "required": ["splice_junctions"]
             }
         ]
     }
diff --git a/docs/usage.md b/docs/usage.md
index f3c0360d0..7e238e8b1 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -83,7 +83,7 @@ nextflow run nf-core/rnafusion  \
   -profile <docker/singularity/.../institute> \
   --references_only \
   --cosmic_username <EMAIL> --cosmic_passwd <PASSWORD> \
-  --fusionreport \
+  --tools fusionreport \
   --genomes_base <PATH/TO/REFERENCES> \
   --outdir <OUTPUT/PATH>
 ```
@@ -111,7 +111,7 @@ The references are only built based on ensembl version 102. It is not possible c
 
 ### Samplesheet input
 
-You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. The pipeline will detect whether a sample is single- or paired-end from the samplesheet - the `fastq_2` column is empty for single-end. The samplesheet has to be a comma-separated file (.csv) but can have as many columns as you desire. There is a strict requirement for the first 4 columns to match those defined in the table below with the header row included.
+You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. The pipeline will detect whether a sample is single- or paired-end from the samplesheet - the `fastq_2` column is empty for single-end. The samplesheet has to be a comma-separated (.csv), tab-separated (.tsv), yaml (.yaml or .yml) or json (.json) file but can have as many columns as you desire. There is a strict requirement for the `sample` and `strandedness` columns. One or more of these columns should be provided too: `fastq_1`, `bam`, `cram`, `junctions` and `splice_junctions`
 A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice.
 
 ```csv title="samplesheet.csv"
@@ -125,14 +125,24 @@ TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,forward
 TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,forward
 ```
 
-As you can see above for multiple runs of the same sample, the `sample` name has to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis.
-
-| Column         | Description                                                                                                                                                                            |
-| -------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
-| `sample`       | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). |
-| `fastq_1`      | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
-| `fastq_2`      | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                             |
-| `strandedness` | Strandedness: forward or reverse.                                                                                                                                                      |
+As you can see above for multiple runs of the same sample, the `sample` name has to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Note that multiple rows per sample are not supported for samples that contain `bam`, `cram`, `junctions` and/or `splice_junctions` files.
+
+| Column             | Description                                                                                                                                                                                                                                                                                                                                                | Required           |
+| ------------------ | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
+| `sample`           | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`).                                                                                                                                                                     | :white_check_mark: |
+| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                          | :white_check_mark: |
+| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ file(s) because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`. | :grey_question:    |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                                                                                                                                                                                     | :x:                |
+| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.             | :grey_question:    |
+| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                              | :x:                |
+| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty.           | :grey_question:    |
+| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                            | :x:                |
+| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.                | :grey_question:    |
+| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                          | :grey_question:    |
+
+:white_check_mark: = Required
+:x: = Not required
+:grey_question: = One of these columns should be provided
 
 ### Starting commands
 
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
index 7f83802e0..02135973e 100644
--- a/subworkflows/local/ctatsplicing_workflow/main.nf
+++ b/subworkflows/local/ctatsplicing_workflow/main.nf
@@ -2,7 +2,7 @@ include { CTATSPLICING_STARTOCANCERINTRONS } from '../../../modules/local/ctatsp
 
 workflow CTATSPLICING_WORKFLOW {
     take:
-    split_junctions // [ val(meta), path(split_junctions.SJ.out.tab) ]
+    splice_junctions // [ val(meta), path(splice_junctions.SJ.out.tab) ]
     junctions       // [ val(meta), path(junctions.Chimeric.out.junction) ]
     aligned_bams    // [ val(meta), path(aligned_bams.Aligned.sortedByCoord.out.bam), path(aligned_bams.Aligned.sortedByCoord.out.bam.bai) ]
     ctat_genome_lib // [ val(meta2), path(path/to/ctat_genome_lib) ]
@@ -10,7 +10,7 @@ workflow CTATSPLICING_WORKFLOW {
     main:
     def ch_versions = Channel.empty()
 
-    def ch_ctatsplicing_input = split_junctions
+    def ch_ctatsplicing_input = splice_junctions
         .join(junctions, failOnMismatch:true, failOnDuplicate:true)
         .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
         .map { meta, split_junction, junction, bam, bai ->
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index e5eefe820..7921f82bf 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -72,7 +72,7 @@ workflow PIPELINE_INITIALISATION {
 
     Channel
         .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json"))
-        .map { meta, fastq_1, fastq_2, bam, bai, cram, crai, junctions, split_junctions, strandedness ->
+        .map { meta, fastq_1, fastq_2, bam, bai, cram, crai, junctions, splice_junctions, strandedness ->
             def meta_fastqs = []
             if (!fastq_1) {
                 meta_fastqs = [ meta, [] ]
@@ -81,15 +81,15 @@ workflow PIPELINE_INITIALISATION {
             } else {
                 meta_fastqs = [ meta + [single_end:false], [ fastq_1, fastq_2 ] ]
             }
-            return [ meta.id ] + meta_fastqs + [ bam, bai, cram, crai, junctions, split_junctions, strandedness ]
+            return [ meta.id ] + meta_fastqs + [ bam, bai, cram, crai, junctions, splice_junctions, strandedness ]
         }
         .groupTuple()
         .map { samplesheet ->
             validateInputSamplesheet(samplesheet)
         }
         .map {
-            meta, fastqs, bam, bai, cram, crai, junctions, split_junctions ->
-                return [ meta, fastqs.flatten(), bam, bai, cram, crai, junctions, split_junctions ]
+            meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ->
+                return [ meta, fastqs.flatten(), bam, bai, cram, crai, junctions, splice_junctions ]
         }
         .set { ch_samplesheet }
 
@@ -168,21 +168,21 @@ def validateInputParameters() {
 // Validate channels from input samplesheet
 //
 def validateInputSamplesheet(input) {
-    def (metas, fastqs, bam, bai, cram, crai, junctions, split_junctions) = input[1..8]
+    def (metas, fastqs, bam, bai, cram, crai, junctions, splice_junctions) = input[1..8]
 
     def bam_list = bam.findAll { it -> it != [] }
     def cram_list = cram.findAll { it -> it != [] }
     def junctions_list = junctions.findAll { it -> it != [] }
-    def split_junctions_list = split_junctions.findAll { it -> it != [] }
+    def splice_junctions_list = splice_junctions.findAll { it -> it != [] }
     // Check alignment and junction files (input is a list)
-    if (bam_list.size() > 1 || cram_list.size() > 1 || junctions_list.size() > 1 || split_junctions_list.size() > 1) {
+    if (bam_list.size() > 1 || cram_list.size() > 1 || junctions_list.size() > 1 || splice_junctions_list.size() > 1) {
         error("Please check input samplesheet -> Only one BAM or CRAM, junctions and split junctions file is allowed per sample: ${metas[0].id}")
     }
 
     bam = bam_list.size() > 0 ? bam_list[0] : []
     cram = cram_list.size() > 0 ? cram_list[0] : []
     junctions = junctions_list.size() > 0 ? junctions_list[0] : []
-    split_junctions = split_junctions_list.size() > 0 ? split_junctions_list[0] : []
+    splice_junctions = splice_junctions_list.size() > 0 ? splice_junctions_list[0] : []
 
     if (bam != [] && cram != []) {
         error("Please check input samplesheet -> Using both BAM and CRAM files isn't allowed: ${metas[0].id}")
@@ -200,7 +200,7 @@ def validateInputSamplesheet(input) {
         error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}")
     }
 
-    return [ metas[0], fastqs, bam, bai.find { it -> it != [] } ?: [], cram, crai.find { it -> it != [] } ?: [], junctions, split_junctions ]
+    return [ metas[0], fastqs, bam, bai.find { it -> it != [] } ?: [], cram, crai.find { it -> it != [] } ?: [], junctions, splice_junctions ]
 }
 
 //
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 4bb34532e..f8b719f87 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -54,10 +54,10 @@ workflow RNAFUSION {
 
     if (!params.references_only) {
 
-        def ch_input = ch_samplesheet.multiMap { meta, fastqs, bam, bai, cram, crai, junctions, split_junctions ->
+        def ch_input = ch_samplesheet.multiMap { meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ->
             def align = false
                 // Check if we need split junctions
-                if (tools.contains("ctatsplicing") && !split_junctions) {
+                if (tools.contains("ctatsplicing") && !splice_junctions) {
                     align = true
                 }
 
@@ -67,7 +67,7 @@ workflow RNAFUSION {
                 }
 
                 // Check if we need BAM or CRAM files
-                if (tools.intersect(["ctatsplicing", "arriba", "stringtie"]) && !bam && !cram) {
+                if (tools.intersect(["ctatsplicing", "arriba", "stringtie", "fusioninspector"]) && !bam && !cram) {
                     align = true
                 }
 
@@ -80,7 +80,7 @@ workflow RNAFUSION {
             bam:                [ new_meta, bam, bai ]
             cram:               [ new_meta, cram, crai ]
             junctions:          [ new_meta, junctions ]
-            split_junctions:    [ new_meta, split_junctions ]
+            splice_junctions:    [ new_meta, splice_junctions ]
         }
 
         // Define which fastqs need to be processes (all analysis that's not aligning)
@@ -171,7 +171,7 @@ workflow RNAFUSION {
         // Add the alignment files to the correct channel if their fastqs aren't aligned
         def ch_aligned_reads        = ch_aligned_inputs
         def ch_star_junctions       = ch_input.junctions.filter { meta, file -> file && !meta.align }
-        def ch_star_split_junctions = ch_input.split_junctions.filter { meta, file -> file && !meta.align }
+        def ch_star_splice_junctions = ch_input.splice_junctions.filter { meta, file -> file && !meta.align }
         if(tools.intersect(["ctatsplicing", "arriba", "starfusion", "stringtie"])) {
             FASTQ_ALIGN_STAR(
                 ch_fastqs_to_align,
@@ -187,7 +187,7 @@ workflow RNAFUSION {
             ch_versions             = ch_versions.mix(FASTQ_ALIGN_STAR.out.versions)
             ch_aligned_reads        = ch_aligned_reads.mix(FASTQ_ALIGN_STAR.out.bam_bai)
             ch_star_junctions       = ch_star_junctions.mix(FASTQ_ALIGN_STAR.out.junctions)
-            ch_star_split_junctions = ch_star_split_junctions.mix(FASTQ_ALIGN_STAR.out.spl_junc_tabs)
+            ch_star_splice_junctions = ch_star_splice_junctions.mix(FASTQ_ALIGN_STAR.out.spl_junc_tabs)
             ch_multiqc_files        = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.log_final.collect{it[1]}.ifEmpty([]))
             ch_multiqc_files        = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.gene_count.collect{it[1]}.ifEmpty([]))
         }
@@ -198,7 +198,7 @@ workflow RNAFUSION {
 
         if(tools.contains("ctatsplicing")) {
             CTATSPLICING_WORKFLOW(
-                ch_star_split_junctions,
+                ch_star_splice_junctions,
                 ch_star_junctions,
                 ch_aligned_reads,
                 BUILD_REFERENCES.out.starfusion_ref

From 94d813038f15dfd065abd7b018d540357536fbaf Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 10 Apr 2025 10:39:43 +0200
Subject: [PATCH 415/887] fix some flow issues

---
 modules/local/fusioninspector/main.nf  |  2 ++
 modules/local/vcf_collect/main.nf      |  4 +++-
 subworkflows/local/build_references.nf |  2 +-
 workflows/rnafusion.nf                 | 20 ++++++++++++--------
 4 files changed, 18 insertions(+), 10 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index ae368b5e7..1eb388578 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -54,6 +54,8 @@ process FUSIONINSPECTOR {
     touch ${prefix}.FusionInspector.fusions.abridged.tsv
     touch ${prefix}.FusionInspector.fusions.tsv
     touch ${prefix}.fusion_inspector_web.html
+    touch ${prefix}.gtf
+    touch ${prefix}.coding_effect
     mkdir -p chckpts_dir
     touch chckpts_dir/add_FFPM.ok
     touch chckpts_dir/add_splice_info.ok
diff --git a/modules/local/vcf_collect/main.nf b/modules/local/vcf_collect/main.nf
index 3a1bf2217..5ea3b39f4 100644
--- a/modules/local/vcf_collect/main.nf
+++ b/modules/local/vcf_collect/main.nf
@@ -19,6 +19,8 @@ process VCF_COLLECT {
     when:
     task.ext.when == null || task.ext.when
 
+    // TODO use BGZIP to compress the VCF file instead of GZIP
+
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
@@ -35,7 +37,7 @@ process VCF_COLLECT {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    touch ${prefix}.vcf
+    touch ${prefix}.vcf.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 8612b8f84..b0307188a 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -165,7 +165,7 @@ workflow BUILD_REFERENCES {
     }
 
     def ch_starfusion_ref = Channel.empty()
-    if (tools.intersect(["starfusion", "ctatsplicing"])) {
+    if (tools.intersect(["starfusion", "ctatsplicing", "fusioninspector"])) {
         if (!exists_not_empty(params.starfusion_ref)) {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index f8b719f87..7afd192fc 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -37,8 +37,8 @@ workflow RNAFUSION {
 
 
     take:
-    ch_samplesheet // channel: samplesheet read in from --input
-    tools          // list: a list of tools to run
+    ch_samplesheet_input    // channel: samplesheet read in from --input
+    tools                   // list: a list of tools to run
 
     main:
 
@@ -54,7 +54,7 @@ workflow RNAFUSION {
 
     if (!params.references_only) {
 
-        def ch_input = ch_samplesheet.multiMap { meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ->
+        def ch_input = ch_samplesheet_input.map { meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ->
             def align = false
                 // Check if we need split junctions
                 if (tools.contains("ctatsplicing") && !splice_junctions) {
@@ -76,11 +76,15 @@ workflow RNAFUSION {
                     error("No fastq files found for ${meta.id}. Either provide fastq files to align or provide a BAM/CRAM file, a junctions file and a split junctions file.")
                 }
             def new_meta = meta + [align:align]
-            fastqs:             [ new_meta, fastqs ]
-            bam:                [ new_meta, bam, bai ]
-            cram:               [ new_meta, cram, crai ]
-            junctions:          [ new_meta, junctions ]
-            splice_junctions:    [ new_meta, splice_junctions ]
+            return [ new_meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ]
+        }
+        .tap { ch_samplesheet }
+        .multiMap { meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ->
+            fastqs:             [ meta, fastqs ]
+            bam:                [ meta, bam, bai ]
+            cram:               [ meta, cram, crai ]
+            junctions:          [ meta, junctions ]
+            splice_junctions:   [ meta, splice_junctions ]
         }
 
         // Define which fastqs need to be processes (all analysis that's not aligning)

From c85a430ccbcc5a4b751f70cc18e1864bbfd0b5d5 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 10 Apr 2025 11:03:10 +0200
Subject: [PATCH 416/887] fix vcf_collect input

---
 modules/local/vcf_collect/main.nf      | 5 ++---
 subworkflows/local/build_references.nf | 8 ++++----
 2 files changed, 6 insertions(+), 7 deletions(-)

diff --git a/modules/local/vcf_collect/main.nf b/modules/local/vcf_collect/main.nf
index 5ea3b39f4..5c1c4ce0e 100644
--- a/modules/local/vcf_collect/main.nf
+++ b/modules/local/vcf_collect/main.nf
@@ -19,10 +19,9 @@ process VCF_COLLECT {
     when:
     task.ext.when == null || task.ext.when
 
-    // TODO use BGZIP to compress the VCF file instead of GZIP
-
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
+    // TODO use BGZIP to compress the VCF file instead of GZIP
     """
     vcf_collect.py --fusioninspector $fusioninspector_tsv --fusionreport $fusionreport_report --fusioninspector_gtf $fusioninspector_gtf_tsv --fusionreport_csv $fusionreport_csv --hgnc $hgnc_ref --sample ${prefix} --out ${prefix}_fusion_data.vcf
     gzip ${prefix}_fusion_data.vcf
@@ -37,7 +36,7 @@ process VCF_COLLECT {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    touch ${prefix}.vcf.gz
+    touch ${prefix}_fusion_data.vcf.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index b0307188a..b54edf900 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -64,14 +64,14 @@ workflow BUILD_REFERENCES {
 
     def ch_hgnc_date = Channel.empty()
     def ch_hgnc_ref  = Channel.empty()
-//TODO: unify as if(tools.contains("fusioninspector")) once nextflow bug fixed
+    //TODO: unify as if(tools.contains("fusioninspector")) once nextflow bug fixed
     def run_fusioninspector = tools.contains("fusioninspector")
-    if(run_fusioninspector) {
+    if(run_fusioninspector && !params.skip_vcf) {
         if ((!exists_not_empty(params.hgnc_ref) || !exists_not_empty(params.hgnc_date)) && !params.skip_vcf){
             HGNC_DOWNLOAD( )
             ch_versions = ch_versions.mix(HGNC_DOWNLOAD.out.versions)
-            ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref
-            ch_hgnc_date = HGNC_DOWNLOAD.out.hgnc_date
+            ch_hgnc_ref = HGNC_DOWNLOAD.out.hgnc_ref.map { that -> [[id:that.Name], that] }
+            ch_hgnc_date = HGNC_DOWNLOAD.out.hgnc_date.map { that -> [[id:that.Name], that] }
         } else {
             ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { that -> [[id:that.Name], that] }
             ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { that -> [[id:that.Name], that] }

From b2b5108755c07a01e71e18d818ced983f4dec01f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 10 Apr 2025 11:10:13 +0200
Subject: [PATCH 417/887] add bam tests

---
 conf/test.config                 |    2 +-
 conf/test_build.config           |    2 +-
 tests/csv/fastq.csv              |    2 +
 tests/test_stub_bam.nf.test      |  339 +++++
 tests/test_stub_bam.nf.test.snap | 1972 ++++++++++++++++++++++++++++++
 tests/yml/bam.yml                |   10 +
 6 files changed, 2325 insertions(+), 2 deletions(-)
 create mode 100644 tests/csv/fastq.csv
 create mode 100644 tests/test_stub_bam.nf.test
 create mode 100644 tests/test_stub_bam.nf.test.snap
 create mode 100644 tests/yml/bam.yml

diff --git a/conf/test.config b/conf/test.config
index e29dc54af..7e5c591ef 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -15,7 +15,7 @@ params {
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
     // Input data
-    input                  = params.pipelines_testdata_base_path +'rnafusion/testdata/human/samplesheet_valid.csv'
+    input                  = "${projectDir}/tests/csv/fastq.csv"
     tools                  = "all"
     no_cosmic              = true
 }
diff --git a/conf/test_build.config b/conf/test_build.config
index 511b83657..aecf79e7d 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -35,7 +35,7 @@ params {
 
     // Input data
     references_only            = true
-    input                      = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
+    input                      = "${projectDir}/tests/csv/fastq.csv"
     no_cosmic                  = true
     tools                      = "arriba,starfusion,fusionreport,salmon,fusioninspector"
     fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'
diff --git a/tests/csv/fastq.csv b/tests/csv/fastq.csv
new file mode 100644
index 000000000..7765b1bf1
--- /dev/null
+++ b/tests/csv/fastq.csv
@@ -0,0 +1,2 @@
+sample,fastq_1,fastq_2,strandedness
+test,https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/reads_1.fq.gz,https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/reads_2.fq.gz,forward
diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
new file mode 100644
index 000000000..4d83cae01
--- /dev/null
+++ b/tests/test_stub_bam.nf.test
@@ -0,0 +1,339 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test"
+    options "-stub" // TODO remove once reference files are available
+    tag "pipeline"
+    tag "pipeline_rnafusion"
+    tag "test_stub_bam"
+
+    test("stub test all") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test arriba") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "arriba"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test ctatsplicing") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "ctatsplicing"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fusioncatcher") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fusioncatcher"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test starfusion") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "starfusion"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test stringtie") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "stringtie"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fusionreport") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fusionreport,arriba,starfusion,fusioncatcher"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fastp") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fastp"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test salmon") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "salmon"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+
+    test("stub test fusioninspector") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fusioninspector,arriba,fusionreport"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
new file mode 100644
index 000000000..c6a9f319e
--- /dev/null
+++ b/tests/test_stub_bam.nf.test.snap
@@ -0,0 +1,1972 @@
+{
+    "stub test starfusion": {
+        "content": [
+            14,
+            {
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-10T10:09:48.999599917"
+    },
+    "stub test stringtie": {
+        "content": [
+            15,
+            {
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-10T10:10:23.893097743"
+    },
+    "stub test arriba": {
+        "content": [
+            14,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.2,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-10T10:07:56.264754749"
+    },
+    "stub test fusioninspector": {
+        "content": [
+            23,
+            {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.0"
+                },
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.4.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONINSPECTOR": {
+                    "STAR-Fusion": null
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "2.1.5"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "2.1.5"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": null
+                },
+                "GET_RRNA_TRANSCRIPTS": {
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+                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/fi_workdir",
+                "fusioninspector/fi_workdir/Log.final.out",
+                "fusioninspector/fi_workdir/Log.out",
+                "fusioninspector/fi_workdir/Log.progress.out",
+                "fusioninspector/fi_workdir/SJ.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome",
+                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/fi_workdir/microH.dat",
+                "fusioninspector/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/fi_workdir/star_align.ok",
+                "fusioninspector/fi_workdir/test.fa",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/fi_workdir/test.gtf",
+                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test.FusionInspector.log",
+                "fusioninspector/test.coding_effect",
+                "fusioninspector/test.fusion_inspector_web.html",
+                "fusioninspector/test.gtf",
+                "fusionreport",
+                "fusionreport/test",
+                "fusionreport/test/AAA_BBB.html",
+                "fusionreport/test/test.fusionreport.tsv",
+                "fusionreport/test/test.fusionreport_filtered.tsv",
+                "fusionreport/test/test.fusions.csv",
+                "fusionreport/test/test.fusions.json",
+                "fusionreport/test/test_fusionreport_index.html",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/fusion_report_db",
+                "references/fusion_report_db/DB-timestamp.txt",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/gffread",
+                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/salmon",
+                "references/salmon/salmon",
+                "references/salmon/salmon/complete_ref_lens.bin",
+                "references/salmon/salmon/ctable.bin",
+                "references/salmon/salmon/ctg_offsets.bin",
+                "references/salmon/salmon/duplicate_clusters.tsv",
+                "references/salmon/salmon/info.json",
+                "references/salmon/salmon/mphf.bin",
+                "references/salmon/salmon/pos.bin",
+                "references/salmon/salmon/pre_indexing.log",
+                "references/salmon/salmon/rank.bin",
+                "references/salmon/salmon/refAccumLengths.bin",
+                "references/salmon/salmon/ref_indexing.log",
+                "references/salmon/salmon/reflengths.bin",
+                "references/salmon/salmon/refseq.bin",
+                "references/salmon/salmon/seq.bin",
+                "references/salmon/salmon/versionInfo.json",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf",
+                "vcf",
+                "vcf/test_fusion_data.vcf.gz"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-10T11:01:03.888007671"
+    }
+}
\ No newline at end of file
diff --git a/tests/yml/bam.yml b/tests/yml/bam.yml
new file mode 100644
index 000000000..345ac7029
--- /dev/null
+++ b/tests/yml/bam.yml
@@ -0,0 +1,10 @@
+- sample: test
+  fastq_1: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/reads_1.fq.gz
+  fastq_2: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/reads_2.fq.gz
+  # bam: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/test.Aligned.sortedByCoord.out.bam
+  # bai: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/test.Aligned.sortedByCoord.out.bam.bai
+  cram: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/test.Aligned.sortedByCoord.out.cram
+  crai: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/test.Aligned.sortedByCoord.out.cram.crai
+  junctions: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/test.Chimeric.out.junction
+  splice_junctions: https://github.com/nf-core/test-datasets/raw/81cb45949e75cbb85cbf6c5ec9009ab45b160823/testdata/human/test.SJ.out.tab
+  strandedness: forward

From fb787e855203c39531ad0f41961216186967c5cb Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 10 Apr 2025 11:18:29 +0200
Subject: [PATCH 418/887] update stub test snap

---
 tests/test_stub.nf.test.snap | 242 ++++++++++++++++++++++++++++++++++-
 1 file changed, 236 insertions(+), 6 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index a94c79609..e976121ed 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -495,17 +495,26 @@
     },
     "stub test fusioninspector": {
         "content": [
-            19,
+            24,
             {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.0"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.4.0"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
+                "FUSIONINSPECTOR": {
+                    "STAR-Fusion": null
+                },
                 "FUSIONREPORT": {
                     "fusion_report": "2.1.5"
                 },
@@ -546,6 +555,9 @@
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "STARFUSION_BUILD": {
+                    "STAR-Fusion": "1.14.0"
+                },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -556,17 +568,145 @@
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "arriba_visualisation",
+                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fusioninspector",
+                "fusioninspector/IGV_inputs",
+                "fusioninspector/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/IGV_inputs/cytoBand.txt",
+                "fusioninspector/IGV_inputs/test.ROI.bed",
+                "fusioninspector/IGV_inputs/test.bed",
+                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/IGV_inputs/test.fa",
+                "fusioninspector/IGV_inputs/test.fa.fai",
+                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/IGV_inputs/test.gtf",
+                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/IGV_inputs/tracks.json",
+                "fusioninspector/chckpts_dir",
+                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/chckpts_dir/blast_filter.ok",
+                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/chckpts_dir/cp_final.ok",
+                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/chckpts_dir/cytoband.ok",
+                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/chckpts_dir/final.abridged.ok",
+                "fusioninspector/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/chckpts_dir/microH.dat.ok",
+                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/chckpts_dir/run_STAR.ok",
+                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/fi_workdir",
+                "fusioninspector/fi_workdir/Log.final.out",
+                "fusioninspector/fi_workdir/Log.out",
+                "fusioninspector/fi_workdir/Log.progress.out",
+                "fusioninspector/fi_workdir/SJ.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome",
+                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/fi_workdir/microH.dat",
+                "fusioninspector/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/fi_workdir/star_align.ok",
+                "fusioninspector/fi_workdir/test.fa",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/fi_workdir/test.gtf",
+                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test.FusionInspector.log",
+                "fusioninspector/test.coding_effect",
+                "fusioninspector/test.fusion_inspector_web.html",
+                "fusioninspector/test.gtf",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -633,6 +773,82 @@
                 "references/star/sjdbList.fromGTF.out.tab",
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -651,14 +867,16 @@
                 "star/test.toTranscriptome.out.bam",
                 "star/test.unmapped_1.fastq.gz",
                 "star/test.unmapped_2.fastq.gz",
-                "star/testXd.out.bam"
+                "star/testXd.out.bam",
+                "vcf",
+                "vcf/test_fusion_data.vcf.gz"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:53:29.478575932"
+        "timestamp": "2025-04-10T11:17:39.031870872"
     },
     "stub test salmon": {
         "content": [
@@ -1386,8 +1604,11 @@
     },
     "stub test all": {
         "content": [
-            34,
+            36,
             {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.0"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -1492,11 +1713,16 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -1650,7 +1876,9 @@
                 "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test.FusionInspector.log",
+                "fusioninspector/test.coding_effect",
                 "fusioninspector/test.fusion_inspector_web.html",
+                "fusioninspector/test.gtf",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -1850,13 +2078,15 @@
                 "stringtie/test.ballgown",
                 "stringtie/test.coverage.gtf",
                 "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
+                "stringtie/test.transcripts.gtf",
+                "vcf",
+                "vcf/test_fusion_data.vcf.gz"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:39:16.250051213"
+        "timestamp": "2025-04-10T11:11:56.869261187"
     }
 }
\ No newline at end of file

From ce5da2b16c334bc08c109932ae3fd5679d599a92 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 10 Apr 2025 11:26:53 +0200
Subject: [PATCH 419/887] update changelog

---
 CHANGELOG.md           |  7 +++++++
 workflows/rnafusion.nf | 38 +++++++++++++++++++-------------------
 2 files changed, 26 insertions(+), 19 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 373d2724d..3bf8819c6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,6 +31,13 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `CTATSPLICING_PREPGENOMELIB` to update the starfusion genome library directory with a cancer splicing index. [#610](https://github.com/nf-core/rnafusion/pull/610)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
 - Add nf-test to local module: `ARRIBA_VISUALISATION`. [#625](https://github.com/nf-core/rnafusion/pull/625)
+- Added the following fields to the samplesheet [#647](https://github.com/nf-core/rnafusion/pull/647):
+  - `bam`: A BAM file aligned with STAR, it's the responsibility of the pipeline user to make sure this file has been correctly called.
+  - `bai`: The index of the BAM file, this is not required when a `bam` file has been given but can increase the pipeline speed a bit.
+  - `cram`: A CRAM file aligned with STAR, it's the responsibility of the pipeline user to make sure this file has been correctly called.
+  - `crai`: The index of the CRAM file, this is not required when a `cram` file has been given but can increase the pipeline speed a bit.
+  - `junctions`: A file containing the junctions determined by STAR (needed by `starfusion` and `ctatsplicing`)
+  - `splice_junctions` A file containing the splice junctions determined by STAR (needed by `ctatsplicing`)
 
 ### Changed
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 7afd192fc..374edc946 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -56,25 +56,25 @@ workflow RNAFUSION {
 
         def ch_input = ch_samplesheet_input.map { meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ->
             def align = false
-                // Check if we need split junctions
-                if (tools.contains("ctatsplicing") && !splice_junctions) {
-                    align = true
-                }
-
-                // Check if we need junctions
-                if (tools.intersect(["starfusion", "ctatsplicing"]) && !junctions) {
-                    align = true
-                }
-
-                // Check if we need BAM or CRAM files
-                if (tools.intersect(["ctatsplicing", "arriba", "stringtie", "fusioninspector"]) && !bam && !cram) {
-                    align = true
-                }
-
-                // Check if there are fastqs when we need to align
-                if (align && !fastqs) {
-                    error("No fastq files found for ${meta.id}. Either provide fastq files to align or provide a BAM/CRAM file, a junctions file and a split junctions file.")
-                }
+            // Check if we need split junctions
+            if (tools.contains("ctatsplicing") && !splice_junctions) {
+                align = true
+            }
+
+            // Check if we need junctions
+            if (tools.intersect(["starfusion", "ctatsplicing"]) && !junctions) {
+                align = true
+            }
+
+            // Check if we need BAM or CRAM files
+            if (tools.intersect(["ctatsplicing", "arriba", "stringtie", "fusioninspector"]) && !bam && !cram) {
+                align = true
+            }
+
+            // Check if there are fastqs when we need to align
+            if (align && !fastqs) {
+                error("No fastq files found for ${meta.id}. Either provide fastq files to align or provide a BAM/CRAM file, a junctions file and a split junctions file.")
+            }
             def new_meta = meta + [align:align]
             return [ new_meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ]
         }

From 9c971965893f2c2b43c6484c72bb3395d1fa9753 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 10 Apr 2025 11:30:18 +0200
Subject: [PATCH 420/887] add new tests to ci

---
 .github/workflows/ci.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 0dcb8fdb0..295c1be94 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -40,6 +40,7 @@ jobs:
         test_profile:
           - "test_stub"
           - "test_build"
+          - "test_stub_bam"
         compute_profile:
           - "docker"
           - "singularity"

From cfb103275c339c1d53b106ce3b6ed3d1e59bd7e4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 10:24:56 +0200
Subject: [PATCH 421/887] remove gtf and coding_effect from fusioninspector
 stub

---
 modules/local/fusioninspector/main.nf | 2 --
 1 file changed, 2 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 1eb388578..ae368b5e7 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -54,8 +54,6 @@ process FUSIONINSPECTOR {
     touch ${prefix}.FusionInspector.fusions.abridged.tsv
     touch ${prefix}.FusionInspector.fusions.tsv
     touch ${prefix}.fusion_inspector_web.html
-    touch ${prefix}.gtf
-    touch ${prefix}.coding_effect
     mkdir -p chckpts_dir
     touch chckpts_dir/add_FFPM.ok
     touch chckpts_dir/add_splice_info.ok

From b47518e2dbb49ce2fc1cccdc7172f64ceb6816c3 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 10:26:36 +0200
Subject: [PATCH 422/887] make fastq_2 required again

---
 assets/schema_input.json | 3 +--
 docs/usage.md            | 4 ++--
 2 files changed, 3 insertions(+), 4 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index 573619ce9..60f8369af 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -76,14 +76,13 @@
             }
         },
         "dependentRequired": {
-            "fastq_2": ["fastq_1"],
             "bai": ["bam"],
             "crai": ["cram"]
         },
         "required": ["sample", "strandedness"],
         "anyOf": [
             {
-                "required": ["fastq_1"]
+                "required": ["fastq_1", "fastq_2"]
             },
             {
                 "required": ["bam"]
diff --git a/docs/usage.md b/docs/usage.md
index 7e238e8b1..a87affb0f 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -131,8 +131,8 @@ As you can see above for multiple runs of the same sample, the `sample` name has
 | ------------------ | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
 | `sample`           | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`).                                                                                                                                                                     | :white_check_mark: |
 | `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                          | :white_check_mark: |
-| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ file(s) because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`. | :grey_question:    |
-| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz".                                                                                                                                                                                                                     | :x:                |
+| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`. | :grey_question:    |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                                                                                                                                                     | :grey_question:                |
 | `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.             | :grey_question:    |
 | `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                              | :x:                |
 | `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty.           | :grey_question:    |

From 7541df52f22fd0132b2262386eb613f7fd3291f9 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 10:27:19 +0200
Subject: [PATCH 423/887] prettier

---
 docs/usage.md | 22 +++++++++++-----------
 1 file changed, 11 insertions(+), 11 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index a87affb0f..4f196bf05 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -127,18 +127,18 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,forward
 
 As you can see above for multiple runs of the same sample, the `sample` name has to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Note that multiple rows per sample are not supported for samples that contain `bam`, `cram`, `junctions` and/or `splice_junctions` files.
 
-| Column             | Description                                                                                                                                                                                                                                                                                                                                                | Required           |
-| ------------------ | ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
-| `sample`           | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`).                                                                                                                                                                     | :white_check_mark: |
-| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                          | :white_check_mark: |
+| Column             | Description                                                                                                                                                                                                                                                                                                                                              | Required           |
+| ------------------ | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
+| `sample`           | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`).                                                                                                                                                                   | :white_check_mark: |
+| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                        | :white_check_mark: |
 | `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`. | :grey_question:    |
-| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                                                                                                                                                     | :grey_question:                |
-| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.             | :grey_question:    |
-| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                              | :x:                |
-| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty.           | :grey_question:    |
-| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                            | :x:                |
-| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.                | :grey_question:    |
-| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                          | :grey_question:    |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz". | :grey_question:    |
+| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.           | :grey_question:    |
+| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                            | :x:                |
+| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty.         | :grey_question:    |
+| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                          | :x:                |
+| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.              | :grey_question:    |
+| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                        | :grey_question:    |
 
 :white_check_mark: = Required
 :x: = Not required

From 491caae4665e00046c9dfb971c6e8220ddd233b2 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 09:25:55 +0000
Subject: [PATCH 424/887] fix snaps

---
 tests/test_stub.nf.test.snap     | 36 ++++++--------------------------
 tests/test_stub_bam.nf.test.snap | 36 ++++++--------------------------
 2 files changed, 12 insertions(+), 60 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index e976121ed..86f939825 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -495,11 +495,8 @@
     },
     "stub test fusioninspector": {
         "content": [
-            24,
+            22,
             {
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.0"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -568,16 +565,11 @@
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "agat",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -704,9 +696,7 @@
                 "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.coding_effect",
                 "fusioninspector/test.fusion_inspector_web.html",
-                "fusioninspector/test.gtf",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -867,16 +857,14 @@
                 "star/test.toTranscriptome.out.bam",
                 "star/test.unmapped_1.fastq.gz",
                 "star/test.unmapped_2.fastq.gz",
-                "star/testXd.out.bam",
-                "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "star/testXd.out.bam"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T11:17:39.031870872"
+        "timestamp": "2025-04-11T09:17:30.682830486"
     },
     "stub test salmon": {
         "content": [
@@ -1604,11 +1592,8 @@
     },
     "stub test all": {
         "content": [
-            36,
+            34,
             {
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.0"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -1713,16 +1698,11 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "agat",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -1876,9 +1856,7 @@
                 "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.coding_effect",
                 "fusioninspector/test.fusion_inspector_web.html",
-                "fusioninspector/test.gtf",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -2078,15 +2056,13 @@
                 "stringtie/test.ballgown",
                 "stringtie/test.coverage.gtf",
                 "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf",
-                "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T11:11:56.869261187"
+        "timestamp": "2025-04-11T09:09:47.739799535"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index c6a9f319e..feb129fc2 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -417,11 +417,8 @@
     },
     "stub test fusioninspector": {
         "content": [
-            23,
+            21,
             {
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.0"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -482,16 +479,11 @@
                     "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "agat",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -618,9 +610,7 @@
                 "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.coding_effect",
                 "fusioninspector/test.fusion_inspector_web.html",
-                "fusioninspector/test.gtf",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -765,16 +755,14 @@
                 "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai",
-                "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "star/test.Aligned.sortedByCoord.out.bam.bai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T11:06:49.393745077"
+        "timestamp": "2025-04-11T09:25:46.143222996"
     },
     "stub test salmon": {
         "content": [
@@ -1508,11 +1496,8 @@
     },
     "stub test all": {
         "content": [
-            35,
+            33,
             {
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.0"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -1609,16 +1594,11 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "agat",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -1772,9 +1752,7 @@
                 "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.coding_effect",
                 "fusioninspector/test.fusion_inspector_web.html",
-                "fusioninspector/test.gtf",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -1958,15 +1936,13 @@
                 "stringtie/test.ballgown",
                 "stringtie/test.coverage.gtf",
                 "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf",
-                "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T11:01:03.888007671"
+        "timestamp": "2025-04-11T09:18:32.616357228"
     }
 }
\ No newline at end of file

From 2854374d4051db620530e0a0c029254054f93808 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 11:44:58 +0200
Subject: [PATCH 425/887] update fusioncatcher to nf-core

---
 conf/modules.config                           | 16 +++-
 modules.json                                  | 10 +++
 .../local/fusioncatcher/build/environment.yml |  5 --
 modules/local/fusioncatcher/build/main.nf     | 45 -----------
 modules/local/fusioncatcher/build/meta.yml    | 24 ------
 .../fusioncatcher/build/environment.yml       |  7 ++
 modules/nf-core/fusioncatcher/build/main.nf   | 45 +++++++++++
 modules/nf-core/fusioncatcher/build/meta.yml  | 47 +++++++++++
 .../fusioncatcher/build/tests/main.nf.test    | 35 +++++++++
 .../build/tests/main.nf.test.snap             | 41 ++++++++++
 .../fusioncatcher/environment.yml             |  7 ++
 .../fusioncatcher/fusioncatcher/main.nf       | 65 ++++++++++++++++
 .../fusioncatcher/fusioncatcher/meta.yml      | 78 +++++++++++++++++++
 .../fusioncatcher/tests/main.nf.test          | 43 ++++++++++
 .../fusioncatcher/tests/main.nf.test.snap     | 73 +++++++++++++++++
 nextflow.config                               |  1 +
 nextflow_schema.json                          |  4 +
 subworkflows/local/build_references.nf        | 12 ++-
 .../local/fusioncatcher_workflow/main.nf      |  9 +--
 19 files changed, 484 insertions(+), 83 deletions(-)
 delete mode 100644 modules/local/fusioncatcher/build/environment.yml
 delete mode 100644 modules/local/fusioncatcher/build/main.nf
 delete mode 100644 modules/local/fusioncatcher/build/meta.yml
 create mode 100644 modules/nf-core/fusioncatcher/build/environment.yml
 create mode 100644 modules/nf-core/fusioncatcher/build/main.nf
 create mode 100644 modules/nf-core/fusioncatcher/build/meta.yml
 create mode 100644 modules/nf-core/fusioncatcher/build/tests/main.nf.test
 create mode 100644 modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
 create mode 100644 modules/nf-core/fusioncatcher/fusioncatcher/main.nf
 create mode 100644 modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
 create mode 100644 modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test
 create mode 100644 modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test.snap

diff --git a/conf/modules.config b/conf/modules.config
index 67b73e49a..fa2118eba 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -87,8 +87,20 @@ process {
         ]
     }
 
-    withName: 'FUSIONCATCHER' {
-        ext.args = "--limitSjdbInsertNsj ${params.fusioncatcher_limitSjdbInsertNsj}"
+    withName: 'FUSIONCATCHER_FUSIONCATCHER' {
+        ext.args = {[
+            "--limitSjdbInsertNsj ${params.fusioncatcher_limitSjdbInsertNsj}",
+            "--skip-blat",
+            meta.single_end ? "--single-end" : "",
+        ].join(' ')}
+    }
+
+    withName: 'FUSIONCATCHER_BUILD' {
+        publishDir = [
+            path: { "${params.genomes_base}/fusioncatcher" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
 
     withName: 'FUSIONCATCHER_DOWNLOAD' {
diff --git a/modules.json b/modules.json
index a0547e179..09978e1bb 100644
--- a/modules.json
+++ b/modules.json
@@ -40,6 +40,16 @@
                         "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
                         "installed_by": ["modules"]
                     },
+                    "fusioncatcher/build": {
+                        "branch": "master",
+                        "git_sha": "5781b9eaa352f23756c3cc98525ee549bbcc612b",
+                        "installed_by": ["modules"]
+                    },
+                    "fusioncatcher/fusioncatcher": {
+                        "branch": "master",
+                        "git_sha": "2a227f75f33415f74a884ef8b33555b151e33de9",
+                        "installed_by": ["modules"]
+                    },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
diff --git a/modules/local/fusioncatcher/build/environment.yml b/modules/local/fusioncatcher/build/environment.yml
deleted file mode 100644
index 8e83de36b..000000000
--- a/modules/local/fusioncatcher/build/environment.yml
+++ /dev/null
@@ -1,5 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
deleted file mode 100644
index db1c72107..000000000
--- a/modules/local/fusioncatcher/build/main.nf
+++ /dev/null
@@ -1,45 +0,0 @@
-process FUSIONCATCHER_BUILD {
-    tag "fusioncatcher_build"
-    label 'process_medium'
-
-    conda "${projectDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
-        'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"
-
-    input:
-    val genome_gencode_version
-
-    output:
-    tuple env(meta), path("human_v${genome_gencode_version}"), emit: reference
-    path "versions.yml"                                      , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-
-    def args = task.ext.args ?: ''
-    meta = [ id: "human_v${genome_gencode_version}" ]
-    """
-    fusioncatcher-build.py \\
-        -g homo_sapiens \\
-        -o human_v${genome_gencode_version} \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    mkdir human_v${genome_gencode_version}
-    touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/fusioncatcher/build/meta.yml b/modules/local/fusioncatcher/build/meta.yml
deleted file mode 100644
index 202be7e17..000000000
--- a/modules/local/fusioncatcher/build/meta.yml
+++ /dev/null
@@ -1,24 +0,0 @@
-name: fusioncatcher_build
-description: Build genome for fusioncatcher
-keywords:
-  - sort
-tools:
-  - fusioncatcher:
-      description: Build genome for fusioncatcher
-      homepage: https://github.com/ndaniel/fusioncatcher/
-      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
-      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
-      doi: "10.1101/011650"
-      licence: ["GPL v3"]
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - reference:
-      type: directory
-      description: Path to fusioncatcher references
-
-authors:
-  - "@praveenraj2018, @rannick"
diff --git a/modules/nf-core/fusioncatcher/build/environment.yml b/modules/nf-core/fusioncatcher/build/environment.yml
new file mode 100644
index 000000000..25f7417c5
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/build/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - "bioconda::fusioncatcher=1.33"
diff --git a/modules/nf-core/fusioncatcher/build/main.nf b/modules/nf-core/fusioncatcher/build/main.nf
new file mode 100644
index 000000000..c24865abb
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/build/main.nf
@@ -0,0 +1,45 @@
+process FUSIONCATCHER_BUILD {
+    tag "${meta.id}"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_5':
+        'biocontainers/fusioncatcher:1.33--hdfd78af_5' }"
+
+    input:
+    val(meta)
+
+    output:
+    tuple val(meta), path("${prefix}")  , emit: reference
+    path "versions.yml"                 , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    fusioncatcher-build \\
+        ${args} \\
+        --output=${prefix} \\
+        --threads=${task.cpus}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
+    END_VERSIONS
+    """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    mkdir ${prefix}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/fusioncatcher/build/meta.yml b/modules/nf-core/fusioncatcher/build/meta.yml
new file mode 100644
index 000000000..54ccb5237
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/build/meta.yml
@@ -0,0 +1,47 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "fusioncatcher_build"
+description: Build references for fusioncatcher
+keywords:
+  - references
+  - fusions
+  - rna
+
+tools:
+  - fusioncatcher:
+      description: Build genome for fusioncatcher
+      homepage: https://github.com/ndaniel/fusioncatcher/
+      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
+      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
+      doi: "10.1101/011650"
+      licence: ["GPL v3"]
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+
+
+output:
+  - reference:
+    - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - ${prefix}:
+        type: file
+        description: Diretory containing the fusioncatcher reference files
+
+  - versions:
+    - "versions.yml":
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+
+authors:
+  - "@nvnieuwk"
+maintainers:
+  - "@nvnieuwk"
diff --git a/modules/nf-core/fusioncatcher/build/tests/main.nf.test b/modules/nf-core/fusioncatcher/build/tests/main.nf.test
new file mode 100644
index 000000000..315c1b117
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/build/tests/main.nf.test
@@ -0,0 +1,35 @@
+nextflow_process {
+
+    name "Test Process FUSIONCATCHER_BUILD"
+    script "../main.nf"
+    process "FUSIONCATCHER_BUILD"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "fusioncatcher"
+    tag "fusioncatcher/build"
+
+    // Only stub tests because this module downloads about 50 GB of data
+
+    test("stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ id:'test', single_end:false ] // meta map
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap b/modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap
new file mode 100644
index 000000000..4a301fcb7
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap
@@ -0,0 +1,41 @@
+{
+    "stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,e049cf8ceb55a41bd8f05db0931a8c14"
+                ],
+                "reference": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e049cf8ceb55a41bd8f05db0931a8c14"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-10T16:50:59.596294661"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/environment.yml b/modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
new file mode 100644
index 000000000..25f7417c5
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - "bioconda::fusioncatcher=1.33"
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
new file mode 100644
index 000000000..5c2a8f38d
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
@@ -0,0 +1,65 @@
+process FUSIONCATCHER_FUSIONCATCHER {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_5':
+        'biocontainers/fusioncatcher:1.33--hdfd78af_5' }"
+
+    input:
+    tuple val(meta), path(fastqs)
+    tuple val(meta2), path(reference)
+
+    output:
+    tuple val(meta), path("*.fusion-genes.txt"), emit: fusions, optional: true
+    tuple val(meta), path("*.summary.txt")     , emit: summary, optional: true
+    tuple val(meta), path("*.log")             , emit: log
+    path "versions.yml"                        , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def input = fastqs.join(",")
+
+    def avail_mem = 3072
+    if (!task.memory) {
+        log.info '[FusionCatcher] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
+    } else {
+        avail_mem = (task.memory.mega*0.8).intValue()
+    }
+    """
+    fusioncatcher \\
+        --input=${input} \\
+        --output=. \\
+        --data=${reference} \\
+        --threads=${task.cpus} \\
+        --Xmx=${avail_mem} \\
+        ${args}
+
+    mv final-list_candidate-fusion-genes.txt ${prefix}.fusion-genes.txt
+    mv summary_candidate_fusions.txt ${prefix}.summary.txt
+    mv fusioncatcher.log ${prefix}.log
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.fusion-genes.txt
+    touch ${prefix}.summary.txt
+    touch ${prefix}.log
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
new file mode 100644
index 000000000..bfd7848ef
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
@@ -0,0 +1,78 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "fusioncatcher_fusioncatcher"
+description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
+keywords:
+  - fusion
+  - rna
+  - fastq
+tools:
+  - fusioncatcher:
+      description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
+      homepage: https://github.com/ndaniel/fusioncatcher
+      documentation: https://github.com/ndaniel/fusioncatcher/wiki
+      tool_dev_url: https://github.com/ndaniel/fusioncatcher
+      doi: "10.1101/011650v1"
+      licence: ["GPL v3"]
+      identifier: biotools:fusioncatcher
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - fastqs:
+        type: list
+        description: A list of input fastq files
+        pattern: "*.{fastq,fq}.gz"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - reference:
+        type: file
+        description: Directory containing fusioncatcher reference files
+
+output:
+  - fusions:
+    - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - "*.fusion-genes.txt":
+        type: file
+        description: The detected fusions in the input files
+        pattern: "*.fusion-genes.txt"
+  - summary:
+    - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - "*.summary.txt":
+        type: file
+        description: The generated summary
+        pattern: "*.summary.txt"
+  - log:
+    - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - "*.log":
+        type: file
+        description: The Fusioncatcher log
+        pattern: "*.log"
+  - versions:
+    - "versions.yml":
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+
+authors:
+  - "@nvnieuwk"
+maintainers:
+  - "@nvnieuwk"
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test b/modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test
new file mode 100644
index 000000000..28e9d13ec
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test
@@ -0,0 +1,43 @@
+nextflow_process {
+
+    name "Test Process FUSIONCATCHER_FUSIONCATCHER"
+    script "../main.nf"
+    process "FUSIONCATCHER_FUSIONCATCHER"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "fusioncatcher"
+    tag "fusioncatcher/fusioncatcher"
+
+    // Only stub tests because the refernce is about 50 GB of data
+
+    test("sarscov2 - fastq - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                ]
+                input[1] = [
+                    [ id:'reference'],
+                    []
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test.snap b/modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test.snap
new file mode 100644
index 000000000..a1916e553
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test.snap
@@ -0,0 +1,73 @@
+{
+    "sarscov2 - fastq - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusion-genes.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,6a54daab2ec7621d8493678e832cf45e"
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusion-genes.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "summary": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6a54daab2ec7621d8493678e832cf45e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-04-11T11:01:13.392512529"
+    }
+}
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index d2635cb46..3ab1abf5e 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -60,6 +60,7 @@ params {
 
     // Download references option
     download_refs              = false
+    fusioncatcher_build        = false
 
     // Path to references
     fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 656f0c2da..e3940c7e1 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -156,6 +156,10 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
+                "fusioncatcher_build": {
+                    "type": "boolean",
+                    "description": "Build fusioncatcher references instead of downloading them"
+                },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 8612b8f84..0f96253ef 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -5,7 +5,7 @@
 */
 
 include { GENCODE_DOWNLOAD }                from '../../modules/local/gencode_download/main'
-include { FUSIONCATCHER_BUILD }             from '../../modules/local/fusioncatcher/build/main'
+include { FUSIONCATCHER_DOWNLOAD }          from '../../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../../modules/local/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../../modules/local/starfusion/build/main'
@@ -18,6 +18,8 @@ include { CTATSPLICING_PREPGENOMELIB }      from '../../modules/local/ctatsplici
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
+
+include { FUSIONCATCHER_BUILD }             from '../../modules/nf-core/fusioncatcher/build/main'
 include { ARRIBA_DOWNLOAD }                 from '../../modules/nf-core/arriba/download/main'
 include { SAMTOOLS_FAIDX }                  from '../../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../../modules/nf-core/star/genomegenerate/main'
@@ -155,9 +157,15 @@ workflow BUILD_REFERENCES {
     def ch_fusioncatcher_ref = Channel.empty()
     if (tools.contains("fusioncatcher")) {
         if (!exists_not_empty(params.fusioncatcher_ref)) {
-                FUSIONCATCHER_BUILD(params.genome_gencode_version)
+            if(params.fusioncatcher_build) {
+                FUSIONCATCHER_BUILD(Channel.value([id:"human_v${params.genome_gencode_version}"]))
                 ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
                 ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
+            } else {
+                FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
+                ch_versions = ch_versions.mix(FUSIONCATCHER_DOWNLOAD.out.versions)
+                ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference
+            }
         }
         else {
             ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { it -> [[id:it.name], it] }
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index f500bc333..f4f053d2f 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -1,9 +1,8 @@
-include { FUSIONCATCHER_DETECT } from '../../../modules/local/fusioncatcher/detect/main'
+include { FUSIONCATCHER_FUSIONCATCHER } from '../../../modules/nf-core/fusioncatcher/fusioncatcher/main'
 
 // TODO: Remove fusioncatcher_fusions as parameter.
 // TODO: remove dummy file. Work with Channel.empty()
 // TODO: if the files were already produced and the user want to skip the module because of this, they should be taken them from the sample sheet
-// TODO: harmonize `fusioncatcher` and `fusioncatcher_only` parameters at main workflow level to activate/skip this one.
 
 workflow FUSIONCATCHER_WORKFLOW {
     take:
@@ -20,12 +19,12 @@ workflow FUSIONCATCHER_WORKFLOW {
                                         .map { meta, _reads, fusions -> [ meta, fusions ] }
         } else {
 
-            FUSIONCATCHER_DETECT (
+            FUSIONCATCHER_FUSIONCATCHER (
                 reads,
                 fusioncatcher_ref
             )
-            ch_fusioncatcher_fusions = FUSIONCATCHER_DETECT.out.fusions
-            ch_versions              = ch_versions.mix(FUSIONCATCHER_DETECT.out.versions)
+            ch_fusioncatcher_fusions = FUSIONCATCHER_FUSIONCATCHER.out.fusions
+            ch_versions              = ch_versions.mix(FUSIONCATCHER_FUSIONCATCHER.out.versions)
         }
 
     emit:

From 3b7827bbce88513fe4de453489fae314d90cff88 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 11:58:42 +0200
Subject: [PATCH 426/887] update changelog + fix linting

---
 CHANGELOG.md                                  |  1 +
 modules/nf-core/fusioncatcher/build/meta.yml  | 25 ++++----
 .../fusioncatcher/fusioncatcher/meta.yml      | 62 +++++++++----------
 3 files changed, 44 insertions(+), 44 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 373d2724d..e9ea97b6b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -78,6 +78,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
 - Fixed the codebase to be compatible with the Nextflow language server [#634](https://github.com/nf-core/rnafusion/pull/634)
 - Updated the input validation to be more strict. This will prevent more errors down the line in the pipeline [#640](https://github.com/nf-core/rnafusion/pull/640)
+- Fusioncatcher build should now work again [#650](https://github.com/nf-core/rnafusion/pull/650)
 
 ### Removed
 
diff --git a/modules/nf-core/fusioncatcher/build/meta.yml b/modules/nf-core/fusioncatcher/build/meta.yml
index 54ccb5237..dc3604e57 100644
--- a/modules/nf-core/fusioncatcher/build/meta.yml
+++ b/modules/nf-core/fusioncatcher/build/meta.yml
@@ -23,23 +23,22 @@ input:
           Groovy Map containing sample information
           e.g. `[ id:'sample1', single_end:false ]`
 
-
 output:
   - reference:
-    - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - ${prefix}:
-        type: file
-        description: Diretory containing the fusioncatcher reference files
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - ${prefix}:
+          type: file
+          description: Diretory containing the fusioncatcher reference files
 
   - versions:
-    - "versions.yml":
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      - "versions.yml":
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 
 authors:
   - "@nvnieuwk"
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
index bfd7848ef..ff6647d65 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
@@ -37,40 +37,40 @@ input:
 
 output:
   - fusions:
-    - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - "*.fusion-genes.txt":
-        type: file
-        description: The detected fusions in the input files
-        pattern: "*.fusion-genes.txt"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.fusion-genes.txt":
+          type: file
+          description: The detected fusions in the input files
+          pattern: "*.fusion-genes.txt"
   - summary:
-    - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - "*.summary.txt":
-        type: file
-        description: The generated summary
-        pattern: "*.summary.txt"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.summary.txt":
+          type: file
+          description: The generated summary
+          pattern: "*.summary.txt"
   - log:
-    - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - "*.log":
-        type: file
-        description: The Fusioncatcher log
-        pattern: "*.log"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.log":
+          type: file
+          description: The Fusioncatcher log
+          pattern: "*.log"
   - versions:
-    - "versions.yml":
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      - "versions.yml":
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 
 authors:
   - "@nvnieuwk"

From 49ca64a350a1ecef27106a112fb17e7c9c98d361 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 14:14:46 +0200
Subject: [PATCH 427/887] fix output issue in fusioncatcher download

---
 .../fusioncatcher/detect/environment.yml      |  5 --
 modules/local/fusioncatcher/detect/main.nf    | 59 -------------------
 modules/local/fusioncatcher/detect/meta.yml   | 53 -----------------
 modules/local/fusioncatcher/download/main.nf  |  4 +-
 4 files changed, 2 insertions(+), 119 deletions(-)
 delete mode 100644 modules/local/fusioncatcher/detect/environment.yml
 delete mode 100644 modules/local/fusioncatcher/detect/main.nf
 delete mode 100644 modules/local/fusioncatcher/detect/meta.yml

diff --git a/modules/local/fusioncatcher/detect/environment.yml b/modules/local/fusioncatcher/detect/environment.yml
deleted file mode 100644
index 8e83de36b..000000000
--- a/modules/local/fusioncatcher/detect/environment.yml
+++ /dev/null
@@ -1,5 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/detect/main.nf b/modules/local/fusioncatcher/detect/main.nf
deleted file mode 100644
index 7980439f8..000000000
--- a/modules/local/fusioncatcher/detect/main.nf
+++ /dev/null
@@ -1,59 +0,0 @@
-process FUSIONCATCHER_DETECT {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "${projectDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
-        'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"
-
-    input:
-    tuple val(meta), path(fastqs, stageAs: "input/*")
-    tuple val(meta2), path(reference)
-
-    output:
-    tuple val(meta), path("*.fusioncatcher.fusion-genes.txt"), emit: fusions, optional: true
-    tuple val(meta), path("*.fusioncatcher.summary.txt")     , emit: summary, optional: true
-    tuple val(meta), path("*.fusioncatcher.log")             , emit: log
-    path "versions.yml"                                      , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args       = task.ext.args ?: ''
-    def prefix     = task.ext.prefix ?: "${meta.id}"
-    def single_end = meta.single_end ? "--single-end" : ""
-    """
-    fusioncatcher.py \\
-        -d ${reference} \\
-        -i input \\
-        -p ${task.cpus} \\
-        -o . \\
-        --skip-blat \\
-        ${single_end} \\
-        ${args}
-
-    mv final-list_candidate-fusion-genes.txt ${prefix}.fusioncatcher.fusion-genes.txt
-    mv summary_candidate_fusions.txt ${prefix}.fusioncatcher.summary.txt
-    mv fusioncatcher.log ${prefix}.fusioncatcher.log
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fusioncatcher: "\$(fusioncatcher.py --version 2>&1 | awk '{print \$2}')"
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-
-    """
-    touch ${prefix}.fusioncatcher.fusion-genes.txt
-    touch ${prefix}.fusioncatcher.summary.txt
-    touch ${prefix}.fusioncatcher.log
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fusioncatcher: "\$(fusioncatcher.py --version 2>&1 | awk '{print \$2}')"
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/fusioncatcher/detect/meta.yml b/modules/local/fusioncatcher/detect/meta.yml
deleted file mode 100644
index 7c8ee425a..000000000
--- a/modules/local/fusioncatcher/detect/meta.yml
+++ /dev/null
@@ -1,53 +0,0 @@
-name: fusioncatcher
-description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
-keywords:
-  - fusioncatcher
-tools:
-  - fusioncatcher:
-      description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
-      homepage: https://github.com/ndaniel/fusioncatcher
-      documentation: https://github.com/ndaniel/fusioncatcher/wiki
-      tool_dev_url: https://github.com/ndaniel/fusioncatcher
-      doi: "10.1101/011650v1"
-      licence: ["GPL v3"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: FASTQ file
-      pattern: "*.{fastq}"
-  - reference:
-      type: directory
-      description: Path to fusioncatcher references
-      pattern: "*"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - fusions:
-      type: file
-      description: Final list of candidate fusion genes
-      pattern: "*.fusioncatcher.fusion-genes.txt"
-  - summary:
-      type: file
-      description: Summary of fusion results
-      pattern: "*.fusioncatcher_summary.txt"
-  - log:
-      type: file
-      description: Log of fusion results
-      pattern: "*.fusioncatcher.log"
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-
-authors:
-  - "@praveenraj2018. @rannick"
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index af585d62c..1261c7b7b 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -11,8 +11,8 @@ process FUSIONCATCHER_DOWNLOAD {
     val genome_gencode_version
 
     output:
-    tuple env(meta), path("*"), emit: reference
-    path "versions.yml"       , emit: versions
+    tuple env(meta), path("human_v*"), emit: reference
+    path "versions.yml"              , emit: versions
 
     when:
     task.ext.when == null || task.ext.when

From f70e1ec9995700dde697ca422790bb6f81f07a86 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 14:45:51 +0200
Subject: [PATCH 428/887] fix conda declaration in fusioncatcher download

---
 modules/local/fusioncatcher/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 1261c7b7b..6ed3112b9 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -2,7 +2,7 @@ process FUSIONCATCHER_DOWNLOAD {
     tag "fusioncatcher_download"
     label 'process_medium'
 
-    conda "${projectDir}/environment.yml"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
         'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"

From 4af85f891e259c11256ea8adc02106d89f214894 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 12:57:41 +0000
Subject: [PATCH 429/887] fix snap

---
 tests/test_stub.nf.test.snap | 51 +++++++++++++++++++-----------------
 1 file changed, 27 insertions(+), 24 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index a94c79609..78159becb 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -993,10 +993,10 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
+                "FUSIONCATCHER_DOWNLOAD": {
                     "fusioncatcher": "1.33"
                 },
-                "FUSIONCATCHER_DETECT": {
+                "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
@@ -1064,11 +1064,9 @@
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -1109,6 +1107,9 @@
                 "references/fusion_report_db/fusion_report.log",
                 "references/fusion_report_db/fusiongdb2.db",
                 "references/fusion_report_db/mitelman.db",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1237,7 +1238,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:43:02.916057311"
+        "timestamp": "2025-04-11T12:53:31.628103017"
     },
     "stub test fastp": {
         "content": [
@@ -1320,10 +1321,10 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
+                "FUSIONCATCHER_DOWNLOAD": {
                     "fusioncatcher": "1.33"
                 },
-                "FUSIONCATCHER_DETECT": {
+                "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -1353,11 +1354,9 @@
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "get",
@@ -1370,6 +1369,9 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1382,7 +1384,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:40:58.774714515"
+        "timestamp": "2025-04-11T12:50:59.896927711"
     },
     "stub test all": {
         "content": [
@@ -1412,10 +1414,10 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
+                "FUSIONCATCHER_DOWNLOAD": {
                     "fusioncatcher": "1.33"
                 },
-                "FUSIONCATCHER_DETECT": {
+                "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONINSPECTOR": {
@@ -1527,11 +1529,9 @@
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
                 "fusioninspector",
                 "fusioninspector/IGV_inputs",
                 "fusioninspector/IGV_inputs/TrinityFusion.bed",
@@ -1691,6 +1691,9 @@
                 "references/fusion_report_db/fusion_report.log",
                 "references/fusion_report_db/fusiongdb2.db",
                 "references/fusion_report_db/mitelman.db",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1857,6 +1860,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:39:16.250051213"
+        "timestamp": "2025-04-11T12:48:58.038119186"
     }
 }
\ No newline at end of file

From 2e39988352e08f52769302bd524549a98d848c35 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 11 Apr 2025 16:46:05 +0200
Subject: [PATCH 430/887] add tests for exists_not_empty

---
 .../main.nf}                                  |  38 +--
 .../tests/empty_folder/empty_file.txt         |   0
 .../build_references/tests/functions.nf.test  | 273 ++++++++++++++++++
 3 files changed, 292 insertions(+), 19 deletions(-)
 rename subworkflows/local/{build_references.nf => build_references/main.nf} (85%)
 create mode 100644 subworkflows/local/build_references/tests/empty_folder/empty_file.txt
 create mode 100644 subworkflows/local/build_references/tests/functions.nf.test

diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references/main.nf
similarity index 85%
rename from subworkflows/local/build_references.nf
rename to subworkflows/local/build_references/main.nf
index b54edf900..137e9806a 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -4,28 +4,28 @@
 ========================================================================================
 */
 
-include { GENCODE_DOWNLOAD }                from '../../modules/local/gencode_download/main'
-include { FUSIONCATCHER_BUILD }             from '../../modules/local/fusioncatcher/build/main'
-include { FUSIONREPORT_DOWNLOAD }           from '../../modules/local/fusionreport/download/main'
-include { HGNC_DOWNLOAD }                   from '../../modules/local/hgnc/main'
-include { STARFUSION_BUILD }                from '../../modules/local/starfusion/build/main'
-include { GTF_TO_REFFLAT }                  from '../../modules/local/uscs/custom_gtftogenepred/main'
-include { GET_RRNA_TRANSCRIPTS }            from '../../modules/local/get_rrna_transcript/main'
-include { CTATSPLICING_PREPGENOMELIB }      from '../../modules/local/ctatsplicing/prepgenomelib/main.nf'
+include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode_download/main'
+include { FUSIONCATCHER_BUILD }             from '../../../modules/local/fusioncatcher/build/main'
+include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/local/fusionreport/download/main'
+include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
+include { STARFUSION_BUILD }                from '../../../modules/local/starfusion/build/main'
+include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
+include { GET_RRNA_TRANSCRIPTS }            from '../../../modules/local/get_rrna_transcript/main'
+include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatsplicing/prepgenomelib/main.nf'
 
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
-include { ARRIBA_DOWNLOAD }                 from '../../modules/nf-core/arriba/download/main'
-include { SAMTOOLS_FAIDX }                  from '../../modules/nf-core/samtools/faidx/main'
-include { STAR_GENOMEGENERATE }             from '../../modules/nf-core/star/genomegenerate/main'
-include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../modules/nf-core/gatk4/createsequencedictionary/main'
-include { GATK4_BEDTOINTERVALLIST }         from '../../modules/nf-core/gatk4/bedtointervallist/main'
-include { SALMON_INDEX }                    from '../../modules/nf-core/salmon/index/main'
-include { GFFREAD }                         from '../../modules/nf-core/gffread/main'
-include { getFileSuffix } from '../../modules/nf-core/cat/cat/main.nf'
+include { ARRIBA_DOWNLOAD }                 from '../../../modules/nf-core/arriba/download/main'
+include { SAMTOOLS_FAIDX }                  from '../../../modules/nf-core/samtools/faidx/main'
+include { STAR_GENOMEGENERATE }             from '../../../modules/nf-core/star/genomegenerate/main'
+include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../../modules/nf-core/gatk4/createsequencedictionary/main'
+include { GATK4_BEDTOINTERVALLIST }         from '../../../modules/nf-core/gatk4/bedtointervallist/main'
+include { SALMON_INDEX }                    from '../../../modules/nf-core/salmon/index/main'
+include { GFFREAD }                         from '../../../modules/nf-core/gffread/main'
+include { getFileSuffix } from '../../../modules/nf-core/cat/cat/main.nf'
 
 /*
 ========================================================================================
@@ -235,9 +235,9 @@ def exists_not_empty(path) {
         return false
     }
 
-    // Check if the file is not a directory or is a URL and return whether it's empty or not
-    def is_url = ["https://", "ftp://", "http://"].findAll { it -> path.startsWith(it) }.size() > 0
-    if(is_url || !path_to_check.toFile().isDirectory()) {
+    // Don't check directories if the path is not local
+    def is_local = path_to_check.getScheme() == "file"
+    if(!is_local || !path_to_check.toFile().isDirectory()) {
         return !path_to_check.isEmpty()
     }
 
diff --git a/subworkflows/local/build_references/tests/empty_folder/empty_file.txt b/subworkflows/local/build_references/tests/empty_folder/empty_file.txt
new file mode 100644
index 000000000..e69de29bb
diff --git a/subworkflows/local/build_references/tests/functions.nf.test b/subworkflows/local/build_references/tests/functions.nf.test
new file mode 100644
index 000000000..2933918eb
--- /dev/null
+++ b/subworkflows/local/build_references/tests/functions.nf.test
@@ -0,0 +1,273 @@
+
+nextflow_function {
+
+    name "Test Functions"
+    script "subworkflows/local/build_references/main.nf"
+
+    test("Test Function exists_not_empty - local file exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "${projectDir}/main.nf"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == true
+        }
+
+    }
+
+    test("Test Function exists_not_empty - local directory exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "${projectDir}/assets"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == true
+        }
+
+    }
+
+    test("Test Function exists_not_empty - local file not exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "${projectDir}/non_existing_file.txt"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+    test("Test Function exists_not_empty - local directory not exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "${projectDir}/non_existing_directory"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+    test("Test Function exists_not_empty - local file empty") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "${projectDir}/subworkflows/local/build_references/tests/empty_folder/empty_file.txt"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+    test("Test Function exists_not_empty - local directory empty") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "${projectDir}/subworkflows/local/build_references/tests/empty_folder/"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+    test("Test Function exists_not_empty - HTTPS file exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "https://github.com/nf-core/test-datasets/raw/afe31d4ea9be70bbaaf1fb633a4ae7a4afeaf215/README.md"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == true
+        }
+
+    }
+
+    test("Test Function exists_not_empty - HTTPS directory exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "https://github.com/nf-core/test-datasets/raw/afe31d4ea9be70bbaaf1fb633a4ae7a4afeaf215/testdata/"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == true
+        }
+
+    }
+
+    test("Test Function exists_not_empty - HTTPS file not exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "https://github.com/nf-core/test-datasets/raw/afe31d4ea9be70bbaaf1fb633a4ae7a4afeaf215/non_existing_file.txt"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+    test("Test Function exists_not_empty - HTTPS directory not exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "https://github.com/nf-core/test-datasets/raw/afe31d4ea9be70bbaaf1fb633a4ae7a4afeaf215/non_existing_directory/"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+    test("Test Function exists_not_empty - s3 file exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "s3://ngi-igenomes/igenomes/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == true
+        }
+
+    }
+
+    test("Test Function exists_not_empty - s3 directory exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "s3://ngi-igenomes/igenomes/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == true
+        }
+
+    }
+
+    test("Test Function exists_not_empty - s3 file not exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "s3://ngi-igenomes/igenomes/this_file_does_not_exist.txt"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+    test("Test Function exists_not_empty - s3 directory not exists") {
+
+        function "exists_not_empty"
+
+        when {
+            function {
+                """
+                input[0] = "s3://ngi-igenomes/igenomes/this_directory_does_not_exist/"
+                """
+            }
+        }
+
+        then {
+            assert function.success
+            assert function.result == false
+        }
+
+    }
+
+}

From 6536e6af7f88ff5cfc063be1d83faa1953a8f1f8 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 14 Apr 2025 10:09:36 +0200
Subject: [PATCH 431/887] add star command to usage docs

---
 docs/usage.md | 61 +++++++++++++++++++++++++++++++++++++++++----------
 1 file changed, 49 insertions(+), 12 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 4f196bf05..5bd348e78 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -127,23 +127,60 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,forward
 
 As you can see above for multiple runs of the same sample, the `sample` name has to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Note that multiple rows per sample are not supported for samples that contain `bam`, `cram`, `junctions` and/or `splice_junctions` files.
 
-| Column             | Description                                                                                                                                                                                                                                                                                                                                              | Required           |
-| ------------------ | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
-| `sample`           | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`).                                                                                                                                                                   | :white_check_mark: |
-| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                        | :white_check_mark: |
-| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`. | :grey_question:    |
-| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz". | :grey_question:    |
-| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.           | :grey_question:    |
-| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                            | :x:                |
-| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty.         | :grey_question:    |
-| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                          | :x:                |
-| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.              | :grey_question:    |
-| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                        | :grey_question:    |
+| Column             | Description                                                                                                                                                                                                                                                                                                                                                                                                                                   | Required           |
+| ------------------ | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
+| `sample`           | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`).                                                                                                                                                                                                                                                        | :white_check_mark: |
+| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                                                                                                             | :white_check_mark: |
+| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.                                                                                      | :grey_question:    |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                      | :grey_question:    |
+| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.   | :grey_question:    |
+| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                                                                                                                 | :x:                |
+| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty. | :grey_question:    |
+| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                                                                                                               | :x:                |
+| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.      | :grey_question:    |
+| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                | :grey_question:    |
 
 :white_check_mark: = Required
 :x: = Not required
 :grey_question: = One of these columns should be provided
 
+### Preparing BAM/CRAM/junctions/splice_junctions
+
+In the pipeline the following STAR command is used to produce the needed files:
+
+```bash
+STAR \\
+    --genomeDir <path-to-star-index> \
+    --readFilesIn <comma-separated-list-of-forward-fastqs> <comma-separated-list-of-reverse-fastqs> \
+    --runThreadN <threads> \
+    --outFileNamePrefix <sample-name>. \
+    --outSAMattrRGline 'ID:<sample-name>' 'SM:<sample-name>' \
+    --outReadsUnmapped None  \
+    --outSAMstrandField intronMotif \
+    --chimOutJunctionFormat 1 \
+    --twopassMode None \
+    --outFilterMultimapNmax 50 \
+    --chimMultimapNmax 50 \
+    --quantMode GeneCounts \
+    --outSAMunmapped Within \
+    --readFilesCommand zcat  \
+    --alignSJstitchMismatchNmax 5 -1 5 5 \
+    --outSAMtype BAM SortedByCoordinate \
+    --chimSegmentMin 10 \
+    --peOverlapNbasesMin 10 \
+    --alignSplicedMateMapLminOverLmate 0.5 \
+    --chimJunctionOverhangMin 10 \
+    --chimScoreJunctionNonGTAG 0 \
+    --chimScoreDropMax 30 \
+    --chimScoreSeparation 1  \
+    --chimSegmentReadGapMax 3 \
+    --chimOutType Junctions WithinBAM'
+```
+
+We found that this command produces the best results for all downstream processes in the pipeline. It is highly recommended to use the same command for the input BAM, CRAM, junctions and splice_junctions files.
+
+The pipeline will still work when another command has been used, but it is possible that the results will be significantly different from the standard flow.
+
 ### Starting commands
 
 The pipeline can either be run using all fusion detection tools or by specifying individual tools. Visualisation tools will be run on all fusions detected. To run all tools (`arriba`, `ctatsplicing`, `fusioncatcher`, `starfusion`, `stringtie`, `fusionreport`, `fastp`, `salmon`, `fusioninspector`) use the `all` option for the `--tools` parameter:

From 101c68df789f1f6efa2174a221709f20aa98930f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 14 Apr 2025 15:23:32 +0200
Subject: [PATCH 432/887] update fusioncatcher download container

---
 modules/local/fusioncatcher/download/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 6ed3112b9..06f3e6e19 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -4,8 +4,8 @@ process FUSIONCATCHER_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d5/d53f36e9e01d14a0ae8e15f8046f52b2883c970c27fe43fdfbd9440a55f5403f/data' :
-        'community.wave.seqera.io/library/fusioncatcher:1.33--4733482b637ef92f' }"
+        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_5':
+        'biocontainers/fusioncatcher:1.33--hdfd78af_5' }"
 
     input:
     val genome_gencode_version

From 692bd78e511cbb232d9d1a1f577a35b6bdb4e0a1 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 14 Apr 2025 15:56:13 +0200
Subject: [PATCH 433/887] fix fusioncatcher download (hopefully)

---
 modules/local/fusioncatcher/download/main.nf |  7 +++----
 nextflow.config                              |  5 +++--
 nextflow_schema.json                         | 11 +++++++++--
 subworkflows/local/build_references.nf       |  2 +-
 4 files changed, 16 insertions(+), 9 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 06f3e6e19..695c34f7b 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,10 +22,9 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.aa
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.ab
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.ac
-    wget $args http://sourceforge.net/projects/fusioncatcher/files/data/human_${genome_gencode_version}.tar.gz.ad
+    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partaa?viasf=1
+    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partab?viasf=1
+    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partac?viasf=1
     cat human_${genome_gencode_version}.tar.gz.* | tar xz
     rm human_${genome_gencode_version}.tar*
 
diff --git a/nextflow.config b/nextflow.config
index 3ab1abf5e..e0d0bcec1 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -59,8 +59,9 @@ params {
     skip_vcf                   = false
 
     // Download references option
-    download_refs              = false
-    fusioncatcher_build        = false
+    download_refs               = false
+    fusioncatcher_download      = false
+    fusioncatcher_download_link = "https://master.dl.sourceforge.net/project/rnafusion/fusioncatcher"
 
     // Path to references
     fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index e3940c7e1..6ce2eb0ef 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -156,9 +156,16 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
-                "fusioncatcher_build": {
+                "fusioncatcher_download": {
                     "type": "boolean",
-                    "description": "Build fusioncatcher references instead of downloading them"
+                    "description": "Download fusioncatcher references instead of building them. This is only supported for human gencode v46 at the moment."
+                },
+                "fusioncatcher_download_link": {
+                    "type": "string",
+                    "description": "Fusioncatcher download link",
+                    "fa_icon": "far fa-file-code",
+                    "pattern": "^https?://.*$",
+                    "default": "https://master.dl.sourceforge.net/project/rnafusion/fusioncatcher"
                 },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 0f96253ef..c67e952ed 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -157,7 +157,7 @@ workflow BUILD_REFERENCES {
     def ch_fusioncatcher_ref = Channel.empty()
     if (tools.contains("fusioncatcher")) {
         if (!exists_not_empty(params.fusioncatcher_ref)) {
-            if(params.fusioncatcher_build) {
+            if(!params.fusioncatcher_download) {
                 FUSIONCATCHER_BUILD(Channel.value([id:"human_v${params.genome_gencode_version}"]))
                 ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
                 ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference

From 71338828f8c7f1f3b8774f1bfdec62f80530a7cc Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 14 Apr 2025 16:04:53 +0200
Subject: [PATCH 434/887] bump changelog

---
 CHANGELOG.md | 18 ++++++++++++++----
 1 file changed, 14 insertions(+), 4 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e9ea97b6b..360d8d923 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,6 +31,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `CTATSPLICING_PREPGENOMELIB` to update the starfusion genome library directory with a cancer splicing index. [#610](https://github.com/nf-core/rnafusion/pull/610)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
 - Add nf-test to local module: `ARRIBA_VISUALISATION`. [#625](https://github.com/nf-core/rnafusion/pull/625)
+- Added `--fusioncatcher_download` and `--fusioncatcher_download_link`. These parameters can be used to download the fusioncatcher references from a repository, which is significantly faster than building the reference. This is only supported for human references for gencode v46.
 
 ### Changed
 
@@ -88,10 +89,19 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Parameters
 
-| Old parameter        | New parameter       |
-| -------------------- | ------------------- |
-|                      | `--no_cosmic`       |
-| `--build_references` | `--references_only` |
+| Old parameter         | New parameter                   |
+| --------------------- | ------------------------------- |
+|                       | `--no_cosmic`                   |
+| `--build_references`  | `--references_only`             |
+| `--fastp_trim`        | `--tools`                       |
+| `--arriba`            | `--tools`                       |
+| `--ctatsplicing`      | `--tools`                       |
+| `--run_fusioncatcher` | `--tools`                       |
+| `--starfusion`        | `--tools`                       |
+| `--stringtie`         | `--tools`                       |
+| `--all`               | `--tools`                       |
+|                       | `--fusioncatcher_download`      |
+|                       | `--fusioncatcher_download_link` |
 
 ## v3.0.2 - [2024-04-10]
 

From a7b0fdd5a85f86129f5a1d8ae2f98c5230cfbcea Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 14 Apr 2025 14:48:41 +0000
Subject: [PATCH 435/887] update snaps

---
 tests/test_stub.nf.test.snap | 15 ++++++---------
 1 file changed, 6 insertions(+), 9 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 78159becb..a4679aa4b 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -993,7 +993,7 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
+                "FUSIONCATCHER_BUILD": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1109,7 +1109,6 @@
                 "references/fusion_report_db/mitelman.db",
                 "references/fusioncatcher",
                 "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1238,7 +1237,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-11T12:53:31.628103017"
+        "timestamp": "2025-04-14T14:43:39.213664473"
     },
     "stub test fastp": {
         "content": [
@@ -1321,7 +1320,7 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
+                "FUSIONCATCHER_BUILD": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1371,7 +1370,6 @@
                 "references",
                 "references/fusioncatcher",
                 "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1384,7 +1382,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-11T12:50:59.896927711"
+        "timestamp": "2025-04-14T14:41:00.743099102"
     },
     "stub test all": {
         "content": [
@@ -1414,7 +1412,7 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
+                "FUSIONCATCHER_BUILD": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1693,7 +1691,6 @@
                 "references/fusion_report_db/mitelman.db",
                 "references/fusioncatcher",
                 "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1860,6 +1857,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-11T12:48:58.038119186"
+        "timestamp": "2025-04-14T14:38:02.64741918"
     }
 }
\ No newline at end of file

From b9301de56657f212ccfe5c6003701829f9ef964a Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 15 Apr 2025 15:09:38 +0200
Subject: [PATCH 436/887] fix fusioncatcher download paths

---
 modules/local/fusioncatcher/download/main.nf | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 695c34f7b..0beabe07d 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,11 +22,11 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partaa?viasf=1
-    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partab?viasf=1
-    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partac?viasf=1
-    cat human_${genome_gencode_version}.tar.gz.* | tar xz
-    rm human_${genome_gencode_version}.tar*
+    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partaa
+    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partab
+    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partac
+    cat human_v${genome_gencode_version}.tar.gz.* | tar xz
+    rm human_v${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From 677fdccbaa41f2091cbf78cb1c5799d38c0f3604 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 15 Apr 2025 15:14:40 +0200
Subject: [PATCH 437/887] revert to fusioncatcher_build

---
 CHANGELOG.md                           | 4 ++--
 nextflow.config                        | 2 +-
 nextflow_schema.json                   | 4 ++--
 subworkflows/local/build_references.nf | 2 +-
 4 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 360d8d923..79d7c6891 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -31,7 +31,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `CTATSPLICING_PREPGENOMELIB` to update the starfusion genome library directory with a cancer splicing index. [#610](https://github.com/nf-core/rnafusion/pull/610)
 - Add nf-test to local subworkflow: `FUSIONREPORT_WORKFLOW`. [#607](https://github.com/nf-core/rnafusion/pull/607)
 - Add nf-test to local module: `ARRIBA_VISUALISATION`. [#625](https://github.com/nf-core/rnafusion/pull/625)
-- Added `--fusioncatcher_download` and `--fusioncatcher_download_link`. These parameters can be used to download the fusioncatcher references from a repository, which is significantly faster than building the reference. This is only supported for human references for gencode v46.
+- Added `--fusioncatcher_build` and `--fusioncatcher_download_link`. [#650](https://github.com/nf-core/rnafusion/pull/650)
 
 ### Changed
 
@@ -100,7 +100,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | `--starfusion`        | `--tools`                       |
 | `--stringtie`         | `--tools`                       |
 | `--all`               | `--tools`                       |
-|                       | `--fusioncatcher_download`      |
+|                       | `--fusioncatcher_build`         |
 |                       | `--fusioncatcher_download_link` |
 
 ## v3.0.2 - [2024-04-10]
diff --git a/nextflow.config b/nextflow.config
index e0d0bcec1..fa9816f66 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -60,7 +60,7 @@ params {
 
     // Download references option
     download_refs               = false
-    fusioncatcher_download      = false
+    fusioncatcher_build         = false
     fusioncatcher_download_link = "https://master.dl.sourceforge.net/project/rnafusion/fusioncatcher"
 
     // Path to references
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 6ce2eb0ef..ae94c4700 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -156,9 +156,9 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
-                "fusioncatcher_download": {
+                "fusioncatcher_build": {
                     "type": "boolean",
-                    "description": "Download fusioncatcher references instead of building them. This is only supported for human gencode v46 at the moment."
+                    "description": "Build fusioncatcher references instead of downloading them. This is required for any references other than for human gencode v46 at the moment."
                 },
                 "fusioncatcher_download_link": {
                     "type": "string",
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index c67e952ed..0f96253ef 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -157,7 +157,7 @@ workflow BUILD_REFERENCES {
     def ch_fusioncatcher_ref = Channel.empty()
     if (tools.contains("fusioncatcher")) {
         if (!exists_not_empty(params.fusioncatcher_ref)) {
-            if(!params.fusioncatcher_download) {
+            if(params.fusioncatcher_build) {
                 FUSIONCATCHER_BUILD(Channel.value([id:"human_v${params.genome_gencode_version}"]))
                 ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
                 ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference

From 0687642a00571fe31340d576c305813e43e95223 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 15 Apr 2025 15:44:42 +0200
Subject: [PATCH 438/887] add no_check_certificate to wgets

---
 modules/local/fusioncatcher/download/main.nf | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 0beabe07d..f3a6b759b 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,9 +22,9 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partaa
-    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partab
-    wget $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partac
+    wget --no-check-certificate $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partaa
+    wget --no-check-certificate $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partab
+    wget --no-check-certificate $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partac
     cat human_v${genome_gencode_version}.tar.gz.* | tar xz
     rm human_v${genome_gencode_version}.tar*
 

From 2e6117c56ab5d97907fcee4db16b896961f69e80 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 15 Apr 2025 14:00:36 +0000
Subject: [PATCH 439/887] fix snap

---
 tests/test_stub.nf.test.snap | 15 +++++++++------
 1 file changed, 9 insertions(+), 6 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index a4679aa4b..afb7258c6 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -993,7 +993,7 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
+                "FUSIONCATCHER_DOWNLOAD": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1109,6 +1109,7 @@
                 "references/fusion_report_db/mitelman.db",
                 "references/fusioncatcher",
                 "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1237,7 +1238,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-14T14:43:39.213664473"
+        "timestamp": "2025-04-15T13:55:57.819598446"
     },
     "stub test fastp": {
         "content": [
@@ -1320,7 +1321,7 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
+                "FUSIONCATCHER_DOWNLOAD": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1370,6 +1371,7 @@
                 "references",
                 "references/fusioncatcher",
                 "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1382,7 +1384,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-14T14:41:00.743099102"
+        "timestamp": "2025-04-15T13:53:38.550933344"
     },
     "stub test all": {
         "content": [
@@ -1412,7 +1414,7 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
+                "FUSIONCATCHER_DOWNLOAD": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1691,6 +1693,7 @@
                 "references/fusion_report_db/mitelman.db",
                 "references/fusioncatcher",
                 "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1857,6 +1860,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-14T14:38:02.64741918"
+        "timestamp": "2025-04-15T13:51:03.612689851"
     }
 }
\ No newline at end of file

From e1f8cbeb26192a4e8c90d5c8a4da906c6b50806c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 15 Apr 2025 16:22:31 +0200
Subject: [PATCH 440/887] fix fusioninspector output

---
 CHANGELOG.md                          | 1 +
 modules/local/fusioninspector/main.nf | 3 +++
 2 files changed, 4 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 373d2724d..99ad7e013 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -78,6 +78,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
 - Fixed the codebase to be compatible with the Nextflow language server [#634](https://github.com/nf-core/rnafusion/pull/634)
 - Updated the input validation to be more strict. This will prevent more errors down the line in the pipeline [#640](https://github.com/nf-core/rnafusion/pull/640)
+- The `FUSIONINSPECTOR` process will no longer fail when no fusions have been found.
 
 ### Removed
 
diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index ae368b5e7..fc5308e8f 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -41,6 +41,9 @@ process FUSIONINSPECTOR {
         --out_prefix $prefix \\
         --vis $args $args2
 
+    # Create the TSV file to avoid output file missing error when the TSV file hasn't been created (when no fusions have been found)
+    touch ${prefix}.FusionInspector.fusions.tsv
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')

From 77fdd0d9b8841482a675c14ff5313fff52f6eeed Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 15 Apr 2025 16:23:31 +0200
Subject: [PATCH 441/887] add pr number

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 99ad7e013..3ce918abf 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -78,7 +78,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
 - Fixed the codebase to be compatible with the Nextflow language server [#634](https://github.com/nf-core/rnafusion/pull/634)
 - Updated the input validation to be more strict. This will prevent more errors down the line in the pipeline [#640](https://github.com/nf-core/rnafusion/pull/640)
-- The `FUSIONINSPECTOR` process will no longer fail when no fusions have been found.
+- The `FUSIONINSPECTOR` process will no longer fail when no fusions have been found. [#651](https://github.com/nf-core/rnafusion/pull/651)
 
 ### Removed
 

From 65cec1548f89e1ce0fad9a1f2133d8a6ad137bae Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 15 Apr 2025 17:10:51 +0000
Subject: [PATCH 442/887] fix: download pfam and dfam databases in starfusion
 build

---
 conf/test_build.config                            |  2 ++
 modules/local/starfusion/build/main.nf            | 15 +++++++++++++--
 modules/local/starfusion/build/meta.yml           |  5 ++++-
 modules/local/starfusion/build/tests/main.nf.test |  6 ++++--
 nextflow.config                                   |  3 ++-
 nextflow_schema.json                              |  2 +-
 subworkflows/local/build_references.nf            |  2 +-
 7 files changed, 27 insertions(+), 8 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index 511b83657..92c753e64 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -42,6 +42,8 @@ params {
     gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
 
     fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+    species                    = 'homo_sapiens'
+    dfam_version               = '3.4'
 
 }
 
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index fcd9cf803..81ab72925 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -12,6 +12,7 @@ process STARFUSION_BUILD {
     tuple val(meta2), path(gtf)
     path fusion_annot_lib
     val dfam_species
+    val dfam_version
 
     output:
     tuple val(meta), path("ctat_genome_lib_build_dir"), emit: reference
@@ -20,11 +21,21 @@ process STARFUSION_BUILD {
     script:
     def args = task.ext.args ?: ''
     """
+    export TMPDIR=/tmp
+    wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
+
+    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3f --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3i --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3m --no-check-certificate
+    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3p --no-check-certificate
+    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
+
     prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
-        --dfam_db ${dfam_species} \\
-        --pfam_db current \\
+        --dfam_db ${dfam_species}_dfam.hmm \\
+        --pfam_db Pfam-A.hmm \\
         --fusion_annot_lib $fusion_annot_lib \\
         --CPU $task.cpus \\
         ${args}
diff --git a/modules/local/starfusion/build/meta.yml b/modules/local/starfusion/build/meta.yml
index d7f65a0b9..1259f2698 100644
--- a/modules/local/starfusion/build/meta.yml
+++ b/modules/local/starfusion/build/meta.yml
@@ -26,7 +26,10 @@ input:
       pattern: "*.dat.gz"
   - dfam_species:
       type: string
-      description: DNA transposable element database (Dfam.hmm), required for repeat masking. Only 'human' or 'mouse' are accepted (will automatically pull the resources from dfam).
+      description: DNA transposable element database (Dfam.hmm), required for repeat masking. Names such as 'homo_sapiens' are accepted (will automatically pull the resources from dfam).
+  - dfam_version:
+      type: string
+      description: DNA transposable element database version, required for repeat masking. Values such as '3.4' are accepted (will automatically pull the resources from dfam).
 
 output:
   - reference:
diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
index 8eb1b6f66..7a98b8e7c 100644
--- a/modules/local/starfusion/build/tests/main.nf.test
+++ b/modules/local/starfusion/build/tests/main.nf.test
@@ -19,7 +19,8 @@ nextflow_process {
                 ]
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
+                input [3] = "homo_sapiens"
+                input [4] = "3.4"
 
                 """
             }
@@ -123,7 +124,8 @@ nextflow_process {
                 ]
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
+                input [3] = "homo_sapiens"
+                input [4] = "3.4"
 
                 """
             }
diff --git a/nextflow.config b/nextflow.config
index d2635cb46..bbf508168 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -31,7 +31,8 @@ params {
     read_length                = 100
     genomes                    = [:]
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
-    species                    = "human"
+    species                    = "homo_sapiens"
+    dfam_version               = '3.4'
 
     // Filtering
     tools_cutoff               = 1
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 656f0c2da..ce6e91a10 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -350,7 +350,7 @@
                 },
                 "species": {
                     "type": "string",
-                    "description": "Which species dfam should automatically download, default: human.",
+                    "description": "Which species dfam should automatically download, default: homo_sapiens.",
                     "fa_icon": "far fa-file-code",
                     "default": "human"
                 }
diff --git a/subworkflows/local/build_references.nf b/subworkflows/local/build_references.nf
index 8612b8f84..23ff3a058 100644
--- a/subworkflows/local/build_references.nf
+++ b/subworkflows/local/build_references.nf
@@ -170,7 +170,7 @@ workflow BUILD_REFERENCES {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
             }
-            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species)
+            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, params.dfam_version)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
                 CTATSPLICING_PREPGENOMELIB(

From 5a2d5fa58934666221c7bb72309d84140e63e316 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 15 Apr 2025 17:52:54 +0000
Subject: [PATCH 443/887] style: fix linting

---
 .../starfusion/build/tests/main.nf.test.snap  | 22 +++++++++++++------
 nextflow_schema.json                          | 10 +++++++--
 2 files changed, 23 insertions(+), 9 deletions(-)

diff --git a/modules/local/starfusion/build/tests/main.nf.test.snap b/modules/local/starfusion/build/tests/main.nf.test.snap
index b08f25197..b647347a1 100644
--- a/modules/local/starfusion/build/tests/main.nf.test.snap
+++ b/modules/local/starfusion/build/tests/main.nf.test.snap
@@ -74,13 +74,15 @@
             "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
             true,
             "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
-            null
+            [
+                "versions.yml:md5,4a6ed47cb345f2a5af4d9f12b7918cdb"
+            ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-12-19T17:03:12.812884291"
+        "timestamp": "2025-04-15T17:34:00.624179533"
     },
     "STARFUSION_BUILD - human - minigenome - stub": {
         "content": [
@@ -170,6 +172,9 @@
                         ]
                     ]
                 ],
+                "1": [
+                    "versions.yml:md5,4a6ed47cb345f2a5af4d9f12b7918cdb"
+                ],
                 "reference": [
                     [
                         {
@@ -254,13 +259,16 @@
                             "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
                         ]
                     ]
+                ],
+                "versions": [
+                    "versions.yml:md5,4a6ed47cb345f2a5af4d9f12b7918cdb"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-12-12T14:57:33.861849482"
+        "timestamp": "2025-04-15T17:34:36.490161525"
     }
-}
\ No newline at end of file
+}
diff --git a/nextflow_schema.json b/nextflow_schema.json
index ce6e91a10..cd48ba16f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -352,7 +352,7 @@
                     "type": "string",
                     "description": "Which species dfam should automatically download, default: homo_sapiens.",
                     "fa_icon": "far fa-file-code",
-                    "default": "human"
+                    "default": "homo_sapiens"
                 }
             }
         },
@@ -543,5 +543,11 @@
         {
             "$ref": "#/$defs/generic_options"
         }
-    ]
+    ],
+    "properties": {
+        "dfam_version": {
+            "type": "number",
+            "default": 3.4
+        }
+    }
 }

From b497b6af09d02e36a7f9fb7f0c1f4359b896440f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 15 Apr 2025 18:21:10 +0000
Subject: [PATCH 444/887] fix: number to string

---
 nextflow_schema.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index cd48ba16f..ceabe6568 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -546,7 +546,7 @@
     ],
     "properties": {
         "dfam_version": {
-            "type": "number",
+            "type": "string",
             "default": 3.4
         }
     }

From b640bfd9c2fce1c71ce1a8bf0b9adcd2cb337ceb Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 15 Apr 2025 18:24:49 +0000
Subject: [PATCH 445/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 373d2724d..29d2fd7ee 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -78,6 +78,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
 - Fixed the codebase to be compatible with the Nextflow language server [#634](https://github.com/nf-core/rnafusion/pull/634)
 - Updated the input validation to be more strict. This will prevent more errors down the line in the pipeline [#640](https://github.com/nf-core/rnafusion/pull/640)
+- Fixed STAR-Fusion build would fail when downloading Pfam and Dfam resources behind SSL. [#653](https://github.com/nf-core/rnafusion/pull/653)
 
 ### Removed
 

From af84af59d60acfb934dd2b2634175dae00223a97 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 16 Apr 2025 09:56:16 +0200
Subject: [PATCH 446/887] touch other outputs

---
 modules/local/fusioninspector/main.nf | 8 +++++++-
 1 file changed, 7 insertions(+), 1 deletion(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index fc5308e8f..d89f252ed 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -41,8 +41,14 @@ process FUSIONINSPECTOR {
         --out_prefix $prefix \\
         --vis $args $args2
 
-    # Create the TSV file to avoid output file missing error when the TSV file hasn't been created (when no fusions have been found)
+    # Touch the output files to make sure they exist
+    touch ${prefix}.FusionInspector.log
+    touch ${prefix}.FusionInspector.fusions.abridged.tsv
     touch ${prefix}.FusionInspector.fusions.tsv
+    touch ${prefix}.fusion_inspector_web.html
+    mkdir -p IGV_inputs
+    mkdir -p fi_workdir
+    mkdir -p chckpts_dir
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From 79c6dd2cad075c2b366a6768fcc9335e566294fc Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 16 Apr 2025 10:03:19 +0200
Subject: [PATCH 447/887] use wget for fusioncatcher download

---
 modules/local/fusioncatcher/download/main.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index f3a6b759b..956bbb0e7 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -4,8 +4,8 @@ process FUSIONCATCHER_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_5':
-        'biocontainers/fusioncatcher:1.33--hdfd78af_5' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3b/3b54fa9135194c72a18d00db6b399c03248103f87e43ca75e4b50d61179994b3/data':
+        'community.wave.seqera.io/library/wget:1.21.4--8b0fcde81c17be5e' }"
 
     input:
     val genome_gencode_version
@@ -30,7 +30,7 @@ process FUSIONCATCHER_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
     END_VERSIONS
     """
 
@@ -40,7 +40,7 @@ process FUSIONCATCHER_DOWNLOAD {
     touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
     END_VERSIONS
     """
 }

From ecd60689464d85c4b3df758e62cbe11ef3a7d425 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 16 Apr 2025 10:05:21 +0200
Subject: [PATCH 448/887] add tar version to fusioncatcher download

---
 modules/local/fusioncatcher/download/main.nf | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 956bbb0e7..f2cc2a3d5 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -31,6 +31,7 @@ process FUSIONCATCHER_DOWNLOAD {
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
+        tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """
 
@@ -41,6 +42,7 @@ process FUSIONCATCHER_DOWNLOAD {
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
+        tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """
 }

From ad900660feb23675e7af3fda9f28dab835f00f52 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 16 Apr 2025 11:28:54 +0200
Subject: [PATCH 449/887] fix snaps

---
 tests/test_stub.nf.test.snap     | 17 +++++----
 tests/test_stub_bam.nf.test.snap | 60 ++++++++++++++++++--------------
 2 files changed, 43 insertions(+), 34 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 5a7627c4e..d58e52dae 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1200,7 +1200,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "fusioncatcher": "1.33"
+                    "wget": "1.21.4",
+                    "tar": 1.35
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1444,7 +1445,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-15T13:55:57.819598446"
+        "timestamp": "2025-04-16T10:39:10.08713305"
     },
     "stub test fastp": {
         "content": [
@@ -1528,7 +1529,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "fusioncatcher": "1.33"
+                    "wget": "1.21.4",
+                    "tar": 1.35
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1590,7 +1592,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-15T13:53:38.550933344"
+        "timestamp": "2025-04-16T10:35:22.7562139"
     },
     "stub test all": {
         "content": [
@@ -1621,7 +1623,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "fusioncatcher": "1.33"
+                    "wget": "1.21.4",
+                    "tar": 1.35
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -2066,6 +2069,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-15T13:51:03.612689851"
+        "timestamp": "2025-04-16T10:32:08.583950433"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index feb129fc2..ac8414578 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -1092,10 +1092,11 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "1.33"
+                "FUSIONCATCHER_DOWNLOAD": {
+                    "wget": "1.21.4",
+                    "tar": 1.35
                 },
-                "FUSIONCATCHER_DETECT": {
+                "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
@@ -1155,11 +1156,9 @@
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -1200,6 +1199,9 @@
                 "references/fusion_report_db/fusion_report.log",
                 "references/fusion_report_db/fusiongdb2.db",
                 "references/fusion_report_db/mitelman.db",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1309,7 +1311,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:11:07.156105951"
+        "timestamp": "2025-04-16T10:51:50.320939135"
     },
     "stub test fastp": {
         "content": [
@@ -1411,10 +1413,11 @@
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "1.33"
+                "FUSIONCATCHER_DOWNLOAD": {
+                    "wget": "1.21.4",
+                    "tar": 1.35
                 },
-                "FUSIONCATCHER_DETECT": {
+                "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -1454,11 +1457,9 @@
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "get",
@@ -1480,6 +1481,9 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1492,7 +1496,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:09:12.170433159"
+        "timestamp": "2025-04-16T10:48:04.746178504"
     },
     "stub test all": {
         "content": [
@@ -1522,10 +1526,11 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_BUILD": {
-                    "fusioncatcher": "1.33"
+                "FUSIONCATCHER_DOWNLOAD": {
+                    "wget": "1.21.4",
+                    "tar": 1.35
                 },
-                "FUSIONCATCHER_DETECT": {
+                "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONINSPECTOR": {
@@ -1629,11 +1634,9 @@
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
-                "fusioncatcher/human_v46",
-                "fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "fusioncatcher/test.fusioncatcher.fusion-genes.txt",
-                "fusioncatcher/test.fusioncatcher.log",
-                "fusioncatcher/test.fusioncatcher.summary.txt",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
                 "fusioninspector",
                 "fusioninspector/IGV_inputs",
                 "fusioninspector/IGV_inputs/TrinityFusion.bed",
@@ -1793,6 +1796,9 @@
                 "references/fusion_report_db/fusion_report.log",
                 "references/fusion_report_db/fusiongdb2.db",
                 "references/fusion_report_db/mitelman.db",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -1943,6 +1949,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-11T09:18:32.616357228"
+        "timestamp": "2025-04-16T10:44:36.932311827"
     }
 }
\ No newline at end of file

From 0db45acefddf31b7f11b2ea1703dc4f653f68eb4 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 16 Apr 2025 17:35:00 +0000
Subject: [PATCH 450/887] chore: apply suggestions from code review

---
 modules/local/starfusion/build/main.nf |  1 -
 nextflow_schema.json                   | 19 +++++++++----------
 2 files changed, 9 insertions(+), 11 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 81ab72925..f66106e2a 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -21,7 +21,6 @@ process STARFUSION_BUILD {
     script:
     def args = task.ext.args ?: ''
     """
-    export TMPDIR=/tmp
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
 
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm --no-check-certificate
diff --git a/nextflow_schema.json b/nextflow_schema.json
index ceabe6568..42a158f9c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -31,7 +31,7 @@
             "type": "object",
             "fa_icon": "fas fa-terminal",
             "description": "Define where the pipeline should find input data and save output data.",
-            "required": ["genomes_base", "outdir", "tools"],
+            "required": ["outdir", "genomes_base", "tools"],
             "properties": {
                 "input": {
                     "type": "string",
@@ -353,6 +353,11 @@
                     "description": "Which species dfam should automatically download, default: homo_sapiens.",
                     "fa_icon": "far fa-file-code",
                     "default": "homo_sapiens"
+                },
+                "dfam_version": {
+                    "type": "string",
+                    "default": "3.4",
+                    "description": "Version of dfam to use"
                 }
             }
         },
@@ -523,10 +528,10 @@
     },
     "allOf": [
         {
-            "$ref": "#/$defs/input_output_options"
+            "$ref": "#/$defs/skip_steps"
         },
         {
-            "$ref": "#/$defs/skip_steps"
+            "$ref": "#/$defs/input_output_options"
         },
         {
             "$ref": "#/$defs/read_trimming_options"
@@ -543,11 +548,5 @@
         {
             "$ref": "#/$defs/generic_options"
         }
-    ],
-    "properties": {
-        "dfam_version": {
-            "type": "string",
-            "default": 3.4
-        }
-    }
+    ]
 }

From acb34a9b8e4a169ff7803ef265bdcd916dad55d5 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 17 Apr 2025 15:00:26 +0200
Subject: [PATCH 451/887] remove fusioncatcher_build

---
 CHANGELOG.md                                |  2 +-
 nextflow.config                             |  1 -
 nextflow_schema.json                        |  4 ----
 subworkflows/local/build_references/main.nf | 12 +++---------
 4 files changed, 4 insertions(+), 15 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 02199cd9f..0600ff6e1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -38,7 +38,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `crai`: The index of the CRAM file, this is not required when a `cram` file has been given but can increase the pipeline speed a bit.
   - `junctions`: A file containing the junctions determined by STAR (needed by `starfusion` and `ctatsplicing`)
   - `splice_junctions` A file containing the splice junctions determined by STAR (needed by `ctatsplicing`)
-- Added `--fusioncatcher_build` and `--fusioncatcher_download_link`. [#650](https://github.com/nf-core/rnafusion/pull/650)
+- Added `--fusioncatcher_download_link`. [#650](https://github.com/nf-core/rnafusion/pull/650)
 
 ### Changed
 
diff --git a/nextflow.config b/nextflow.config
index fa9816f66..14d7162df 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -60,7 +60,6 @@ params {
 
     // Download references option
     download_refs               = false
-    fusioncatcher_build         = false
     fusioncatcher_download_link = "https://master.dl.sourceforge.net/project/rnafusion/fusioncatcher"
 
     // Path to references
diff --git a/nextflow_schema.json b/nextflow_schema.json
index ae94c4700..827d966ca 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -156,10 +156,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
-                "fusioncatcher_build": {
-                    "type": "boolean",
-                    "description": "Build fusioncatcher references instead of downloading them. This is required for any references other than for human gencode v46 at the moment."
-                },
                 "fusioncatcher_download_link": {
                     "type": "string",
                     "description": "Fusioncatcher download link",
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index dd2c5d30c..460a95fd1 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -157,15 +157,9 @@ workflow BUILD_REFERENCES {
     def ch_fusioncatcher_ref = Channel.empty()
     if (tools.contains("fusioncatcher")) {
         if (!exists_not_empty(params.fusioncatcher_ref)) {
-            if(params.fusioncatcher_build) {
-                FUSIONCATCHER_BUILD(Channel.value([id:"human_v${params.genome_gencode_version}"]))
-                ch_versions = ch_versions.mix(FUSIONCATCHER_BUILD.out.versions)
-                ch_fusioncatcher_ref = FUSIONCATCHER_BUILD.out.reference
-            } else {
-                FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
-                ch_versions = ch_versions.mix(FUSIONCATCHER_DOWNLOAD.out.versions)
-                ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference
-            }
+            FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
+            ch_versions = ch_versions.mix(FUSIONCATCHER_DOWNLOAD.out.versions)
+            ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference
         }
         else {
             ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { it -> [[id:it.name], it] }

From a4665a5fad104184fbae7f645350b4cd4409d5cb Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Apr 2025 11:15:14 +0200
Subject: [PATCH 452/887] review comments

---
 CHANGELOG.md                                  |  16 +--
 conf/modules.config                           |   8 --
 modules.json                                  | 135 +++++++++++++-----
 .../fusioncatcher/build/environment.yml       |   7 -
 modules/nf-core/fusioncatcher/build/main.nf   |  45 ------
 modules/nf-core/fusioncatcher/build/meta.yml  |  46 ------
 .../fusioncatcher/build/tests/main.nf.test    |  35 -----
 .../build/tests/main.nf.test.snap             |  41 ------
 8 files changed, 104 insertions(+), 229 deletions(-)
 delete mode 100644 modules/nf-core/fusioncatcher/build/environment.yml
 delete mode 100644 modules/nf-core/fusioncatcher/build/main.nf
 delete mode 100644 modules/nf-core/fusioncatcher/build/meta.yml
 delete mode 100644 modules/nf-core/fusioncatcher/build/tests/main.nf.test
 delete mode 100644 modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 690122180..ce6860041 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -86,7 +86,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
 - Fixed the codebase to be compatible with the Nextflow language server [#634](https://github.com/nf-core/rnafusion/pull/634)
 - Updated the input validation to be more strict. This will prevent more errors down the line in the pipeline [#640](https://github.com/nf-core/rnafusion/pull/640)
-- Fusioncatcher build should now work again [#650](https://github.com/nf-core/rnafusion/pull/650)
 - The `FUSIONINSPECTOR` process will no longer fail when no fusions have been found. [#651](https://github.com/nf-core/rnafusion/pull/651)
 - Fixed STAR-Fusion build would fail when downloading Pfam and Dfam resources behind SSL. [#653](https://github.com/nf-core/rnafusion/pull/653)
 
@@ -95,6 +94,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Remove fusionGDB from documentation and fusion-report download stubs [#503](https://github.com/nf-core/rnafusion/pull/503)
 - Removed test-build as reference building gets integrated in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Removed parameter `--build_references`
+- Removed fusioncatcher build [#650](https://github.com/nf-core/rnafusion/pull/650)
 
 ### Parameters
 
@@ -102,14 +102,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | --------------------- | ------------------------------- |
 |                       | `--no_cosmic`                   |
 | `--build_references`  | `--references_only`             |
-| `--fastp_trim`        | `--tools`                       |
-| `--arriba`            | `--tools`                       |
-| `--ctatsplicing`      | `--tools`                       |
-| `--run_fusioncatcher` | `--tools`                       |
-| `--starfusion`        | `--tools`                       |
-| `--stringtie`         | `--tools`                       |
-| `--all`               | `--tools`                       |
-|                       | `--fusioncatcher_build`         |
+| `--fastp_trim`        | `--tools fastp`                 |
+| `--arriba`            | `--tools arriba`                |
+| `--run_fusioncatcher` | `--tools fusioncatcher`         |
+| `--starfusion`        | `--tools starfusion`            |
+| `--stringtie`         | `--tools stringtie`             |
+| `--all`               | `--tools all`                   |
 |                       | `--fusioncatcher_download_link` |
 
 ## v3.0.2 - [2024-04-10]
diff --git a/conf/modules.config b/conf/modules.config
index fa2118eba..b9cf58749 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -95,14 +95,6 @@ process {
         ].join(' ')}
     }
 
-    withName: 'FUSIONCATCHER_BUILD' {
-        publishDir = [
-            path: { "${params.genomes_base}/fusioncatcher" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
     withName: 'FUSIONCATCHER_DOWNLOAD' {
         publishDir = [
             path: { "${params.genomes_base}/fusioncatcher" },
diff --git a/modules.json b/modules.json
index 09978e1bb..2b71ee8af 100644
--- a/modules.json
+++ b/modules.json
@@ -8,153 +8,206 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "1719e20e3881b37b9e1e8498ea669a2b92e54b9a",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/download": {
                         "branch": "master",
                         "git_sha": "467c202a876d26af544fa8c4b22a050a535462a7",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "a1abf90966a2a4016d3c3e41e228bfcbd4811ccc",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
-                        "installed_by": ["modules"]
-                    },
-                    "fusioncatcher/build": {
-                        "branch": "master",
-                        "git_sha": "5781b9eaa352f23756c3cc98525ee549bbcc612b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "2a227f75f33415f74a884ef8b33555b151e33de9",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "bd5f75ccaf2345269810e66e85de8a70e4de8764",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "rrnatranscripts": {
                         "branch": "master",
                         "git_sha": "812edf8cf702de42d2d8c7314d6f03b97e20abeb",
-                        "installed_by": ["modules"],
+                        "installed_by": [
+                            "modules"
+                        ],
                         "patch": "modules/nf-core/rrnatranscripts/rrnatranscripts.diff"
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "85b5f8a0d9df9ce7587af50e2ee75b37c97515c6",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/convert": {
                         "branch": "master",
                         "git_sha": "b823b7220bac94079f9ea65113401956ca7d633e",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "b7800db9b069ed505db3f9d91b8c72faea9be17b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "2d20463181b1c38981a02e90d3084b5f9fa8d540",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "a5ad53288c79fa52c5ae708c317e09ec2dd149ab",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -163,20 +216,26 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/fusioncatcher/build/environment.yml b/modules/nf-core/fusioncatcher/build/environment.yml
deleted file mode 100644
index 25f7417c5..000000000
--- a/modules/nf-core/fusioncatcher/build/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - "bioconda::fusioncatcher=1.33"
diff --git a/modules/nf-core/fusioncatcher/build/main.nf b/modules/nf-core/fusioncatcher/build/main.nf
deleted file mode 100644
index c24865abb..000000000
--- a/modules/nf-core/fusioncatcher/build/main.nf
+++ /dev/null
@@ -1,45 +0,0 @@
-process FUSIONCATCHER_BUILD {
-    tag "${meta.id}"
-    label 'process_medium'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_5':
-        'biocontainers/fusioncatcher:1.33--hdfd78af_5' }"
-
-    input:
-    val(meta)
-
-    output:
-    tuple val(meta), path("${prefix}")  , emit: reference
-    path "versions.yml"                 , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    fusioncatcher-build \\
-        ${args} \\
-        --output=${prefix} \\
-        --threads=${task.cpus}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
-    END_VERSIONS
-    """
-
-    stub:
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    mkdir ${prefix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fusioncatcher: "\$(fusioncatcher --version 2>&1 | awk '{print \$2}')"
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/fusioncatcher/build/meta.yml b/modules/nf-core/fusioncatcher/build/meta.yml
deleted file mode 100644
index dc3604e57..000000000
--- a/modules/nf-core/fusioncatcher/build/meta.yml
+++ /dev/null
@@ -1,46 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
-name: "fusioncatcher_build"
-description: Build references for fusioncatcher
-keywords:
-  - references
-  - fusions
-  - rna
-
-tools:
-  - fusioncatcher:
-      description: Build genome for fusioncatcher
-      homepage: https://github.com/ndaniel/fusioncatcher/
-      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
-      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
-      doi: "10.1101/011650"
-      licence: ["GPL v3"]
-
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-
-output:
-  - reference:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. `[ id:'sample1', single_end:false ]`
-      - ${prefix}:
-          type: file
-          description: Diretory containing the fusioncatcher reference files
-
-  - versions:
-      - "versions.yml":
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-
-authors:
-  - "@nvnieuwk"
-maintainers:
-  - "@nvnieuwk"
diff --git a/modules/nf-core/fusioncatcher/build/tests/main.nf.test b/modules/nf-core/fusioncatcher/build/tests/main.nf.test
deleted file mode 100644
index 315c1b117..000000000
--- a/modules/nf-core/fusioncatcher/build/tests/main.nf.test
+++ /dev/null
@@ -1,35 +0,0 @@
-nextflow_process {
-
-    name "Test Process FUSIONCATCHER_BUILD"
-    script "../main.nf"
-    process "FUSIONCATCHER_BUILD"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "fusioncatcher"
-    tag "fusioncatcher/build"
-
-    // Only stub tests because this module downloads about 50 GB of data
-
-    test("stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [ id:'test', single_end:false ] // meta map
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap b/modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap
deleted file mode 100644
index 4a301fcb7..000000000
--- a/modules/nf-core/fusioncatcher/build/tests/main.nf.test.snap
+++ /dev/null
@@ -1,41 +0,0 @@
-{
-    "stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            
-                        ]
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,e049cf8ceb55a41bd8f05db0931a8c14"
-                ],
-                "reference": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            
-                        ]
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,e049cf8ceb55a41bd8f05db0931a8c14"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
-        },
-        "timestamp": "2025-04-10T16:50:59.596294661"
-    }
-}
\ No newline at end of file

From 31c9371c11c4db502f5ae9245e3132780ddbe508 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Apr 2025 11:17:23 +0200
Subject: [PATCH 453/887] lint

---
 modules.json                                | 130 +++++---------------
 subworkflows/local/build_references/main.nf |   1 -
 2 files changed, 33 insertions(+), 98 deletions(-)

diff --git a/modules.json b/modules.json
index 2b71ee8af..04fcccc52 100644
--- a/modules.json
+++ b/modules.json
@@ -8,206 +8,148 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "1719e20e3881b37b9e1e8498ea669a2b92e54b9a",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/download": {
                         "branch": "master",
                         "git_sha": "467c202a876d26af544fa8c4b22a050a535462a7",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "a1abf90966a2a4016d3c3e41e228bfcbd4811ccc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "2a227f75f33415f74a884ef8b33555b151e33de9",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "bd5f75ccaf2345269810e66e85de8a70e4de8764",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "rrnatranscripts": {
                         "branch": "master",
                         "git_sha": "812edf8cf702de42d2d8c7314d6f03b97e20abeb",
-                        "installed_by": [
-                            "modules"
-                        ],
+                        "installed_by": ["modules"],
                         "patch": "modules/nf-core/rrnatranscripts/rrnatranscripts.diff"
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "85b5f8a0d9df9ce7587af50e2ee75b37c97515c6",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/convert": {
                         "branch": "master",
                         "git_sha": "b823b7220bac94079f9ea65113401956ca7d633e",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "b7800db9b069ed505db3f9d91b8c72faea9be17b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
                         "git_sha": "2d20463181b1c38981a02e90d3084b5f9fa8d540",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "a5ad53288c79fa52c5ae708c317e09ec2dd149ab",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -216,26 +158,20 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 1eb0eba33..30a26db36 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -19,7 +19,6 @@ include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatspl
 ========================================================================================
 */
 
-include { FUSIONCATCHER_BUILD }             from '../../../modules/nf-core/fusioncatcher/build/main'
 include { ARRIBA_DOWNLOAD }                 from '../../../modules/nf-core/arriba/download/main'
 include { SAMTOOLS_FAIDX }                  from '../../../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../../../modules/nf-core/star/genomegenerate/main'

From b12a8a4d68d41d5abf439ad829110aa7c122d362 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Apr 2025 16:48:25 +0200
Subject: [PATCH 454/887] add seq_center and seq_platform to the samplesheet

---
 assets/schema_input.json                    | 12 ++++++++++++
 conf/modules.config                         |  5 +++--
 docs/usage.md                               |  2 ++
 subworkflows/local/fastq_align_star/main.nf |  6 ++----
 workflows/rnafusion.nf                      |  4 +---
 5 files changed, 20 insertions(+), 9 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index 60f8369af..83684a04a 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -73,6 +73,18 @@
                 "type": "string",
                 "enum": ["forward", "reverse", "unstranded", "unknown"],
                 "errorMessage": "Strandedness has to be 'forward', 'reverse', 'unstranded' or 'unknown'"
+            },
+            "seq_platform": {
+                "type": "string",
+                "pattern": "^\\S+$",
+                "errorMessage": "Sequencing platform has to be a string and cannot contain spaces",
+                "meta": "seq_platform"
+            },
+            "seq_center": {
+                "type": "string",
+                "pattern": "^\\S+$",
+                "errorMessage": "Sequencing center has to be a string and cannot contain spaces",
+                "meta": "seq_center"
             }
         },
         "dependentRequired": {
diff --git a/conf/modules.config b/conf/modules.config
index b9cf58749..6314217e2 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -239,7 +239,8 @@ process {
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
-        ext.args = '--outReadsUnmapped None  \
+        ext.args = { "--outReadsUnmapped None  \
+            --outSAMattrRGline 'ID:${meta.id}' 'SM:${meta.id}'${meta.seq_center ? " 'CN:${meta.seq_center}'": ''}${meta.seq_platform ? "' PL:${meta.seq_platform}'": ''} \
             --outSAMstrandField intronMotif \
             --chimOutJunctionFormat 1 \
             --twopassMode None \
@@ -258,7 +259,7 @@ process {
             --chimScoreDropMax 30 \
             --chimScoreSeparation 1  \
             --chimSegmentReadGapMax 3 \
-            --chimOutType Junctions WithinBAM'
+            --chimOutType Junctions WithinBAM" }
     }
 
     withName: 'STAR_GENOMEGENERATE' {
diff --git a/docs/usage.md b/docs/usage.md
index 5bd348e78..ae5fe8f58 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -139,6 +139,8 @@ As you can see above for multiple runs of the same sample, the `sample` name has
 | `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                                                                                                               | :x:                |
 | `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.      | :grey_question:    |
 | `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                | :grey_question:    |
+| `seq_platform`     | The sequencing platform used to create to sequence the data in the FASTQ files. This value will take precedence over the value provided with `--seq_platform`.                                                                                                                                                                                                                                                                                | :x:                |
+| `seq_center`       | The sequencing center in which the data in the FASTQ files was sequenced. This value will take precedence over the value provided with `--seq_center`.                                                                                                                                                                                                                                                                                        | :x:                |
 
 :white_check_mark: = Required
 :x: = Not required
diff --git a/subworkflows/local/fastq_align_star/main.nf b/subworkflows/local/fastq_align_star/main.nf
index 194c0c29c..9f4620416 100644
--- a/subworkflows/local/fastq_align_star/main.nf
+++ b/subworkflows/local/fastq_align_star/main.nf
@@ -10,8 +10,6 @@ workflow FASTQ_ALIGN_STAR {
     fasta           // channel: [ meta, fasta ]
     fai             // channel: [ meta, fai ]
     ignore_sjdbgtf  // boolean
-    seq_platform    // string
-    seq_center      // string
     cram            // boolean: Create CRAM files
 
     main:
@@ -21,8 +19,8 @@ workflow FASTQ_ALIGN_STAR {
         index,
         gtf,
         ignore_sjdbgtf,
-        seq_platform,
-        seq_center
+        "", // seq_platform is handled in the config
+        ""  // seq_center is handled in the config
     )
     ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first())
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 374edc946..bda6500af 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -75,7 +75,7 @@ workflow RNAFUSION {
             if (align && !fastqs) {
                 error("No fastq files found for ${meta.id}. Either provide fastq files to align or provide a BAM/CRAM file, a junctions file and a split junctions file.")
             }
-            def new_meta = meta + [align:align]
+            def new_meta = meta + [align:align, seq_center:meta.seq_center ?: params.seq_center, seq_platform:meta.seq_platform ?: params.seq_platform]
             return [ new_meta, fastqs, bam, bai, cram, crai, junctions, splice_junctions ]
         }
         .tap { ch_samplesheet }
@@ -184,8 +184,6 @@ workflow RNAFUSION {
                 BUILD_REFERENCES.out.fasta,
                 BUILD_REFERENCES.out.fai,
                 params.star_ignore_sjdbgtf,
-                params.seq_platform,
-                params.seq_center,
                 params.cram
             )
             ch_versions             = ch_versions.mix(FASTQ_ALIGN_STAR.out.versions)

From ae125b7fada350ec91aeef00deb567144dbb129b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Apr 2025 16:51:10 +0200
Subject: [PATCH 455/887] changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index ce6860041..5973096f8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -39,6 +39,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `junctions`: A file containing the junctions determined by STAR (needed by `starfusion` and `ctatsplicing`)
   - `splice_junctions` A file containing the splice junctions determined by STAR (needed by `ctatsplicing`)
 - Added `--fusioncatcher_download_link`. [#650](https://github.com/nf-core/rnafusion/pull/650)
+- Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
 
 ### Changed
 

From 1638c5fa9e742aa8f944575289ae2a33f4d0fc03 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 23 Apr 2025 10:36:05 +0200
Subject: [PATCH 456/887] update wget to conda-forge

---
 modules/local/gencode_download/main.nf | 8 ++++----
 modules/local/hgnc/main.nf             | 6 +++---
 modules/local/starfusion/build/main.nf | 2 ++
 3 files changed, 9 insertions(+), 7 deletions(-)

diff --git a/modules/local/gencode_download/main.nf b/modules/local/gencode_download/main.nf
index 1f466d264..a3308e827 100644
--- a/modules/local/gencode_download/main.nf
+++ b/modules/local/gencode_download/main.nf
@@ -4,8 +4,8 @@ process GENCODE_DOWNLOAD {
 
     conda "bioconda::gnu-wget=1.18"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
-        'quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3b/3b54fa9135194c72a18d00db6b399c03248103f87e43ca75e4b50d61179994b3/data' :
+        'community.wave.seqera.io/library/wget:1.21.4--8b0fcde81c17be5e' }"
 
     input:
     val genome_gencode_version
@@ -24,9 +24,9 @@ process GENCODE_DOWNLOAD {
     def folder_gencode = genome.contains("38") ? "" : "/${genome}_mapping"
     def gtf_file_name = genome.contains("38") ? "gencode.v${genome_gencode_version}.primary_assembly.annotation.gtf.gz" : "gencode.v${genome_gencode_version}lift${genome_gencode_version}.annotation.gtf.gz"
     """
-    wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_${genome_gencode_version}/${folder_gencode}${genome}.primary_assembly.genome.fa.gz -O Homo_sapiens_${genome}_${genome_gencode_version}_dna_primary_assembly.fa.gz
+    wget --no-check-certificate ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_${genome_gencode_version}/${folder_gencode}${genome}.primary_assembly.genome.fa.gz -O Homo_sapiens_${genome}_${genome_gencode_version}_dna_primary_assembly.fa.gz
     gunzip Homo_sapiens_${genome}_${genome_gencode_version}_dna_primary_assembly.fa.gz
-    wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_${genome_gencode_version}/${folder_gencode}${gtf_file_name} -O Homo_sapiens_${genome}_${genome_gencode_version}.gtf.gz
+    wget --no-check-certificate ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_${genome_gencode_version}/${folder_gencode}${gtf_file_name} -O Homo_sapiens_${genome}_${genome_gencode_version}.gtf.gz
     gunzip  Homo_sapiens_${genome}_${genome_gencode_version}.gtf.gz
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index aa5c077cc..0b4e4a2f5 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -4,8 +4,8 @@ process HGNC_DOWNLOAD {
 
     conda "bioconda::gnu-wget=1.18"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gnu-wget:1.18--h5bf99c6_5' :
-        'biocontainers/gnu-wget:1.18--h5bf99c6_5' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3b/3b54fa9135194c72a18d00db6b399c03248103f87e43ca75e4b50d61179994b3/data' :
+        'community.wave.seqera.io/library/wget:1.21.4--8b0fcde81c17be5e' }"
 
     output:
     path "hgnc_complete_set.txt"        , emit: hgnc_ref
@@ -15,7 +15,7 @@ process HGNC_DOWNLOAD {
 
     script:
     """
-    wget https://storage.googleapis.com/public-download-files/hgnc/tsv/tsv/hgnc_complete_set.txt
+    wget --no-check-certificate https://storage.googleapis.com/public-download-files/hgnc/tsv/tsv/hgnc_complete_set.txt
     date +%Y-%m-%d/%H:%M  > HGNC-DB-timestamp.txt
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index f66106e2a..ada898bc8 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -41,6 +41,7 @@ process STARFUSION_BUILD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
         STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
     END_VERSIONS
     """
@@ -132,6 +133,7 @@ process STARFUSION_BUILD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
+        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
         STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
     END_VERSIONS
     """

From 14c2c88f20381b171d87a8538cdad6101c31e67e Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 23 Apr 2025 10:52:30 +0200
Subject: [PATCH 457/887] bump changelog, fix tests

---
 CHANGELOG.md                           |  1 +
 modules/local/gencode_download/main.nf |  4 +--
 modules/local/hgnc/main.nf             |  4 +--
 modules/local/starfusion/build/main.nf |  4 +--
 tests/test_stub.nf.test.snap           | 49 ++++++++++++++------------
 5 files changed, 34 insertions(+), 28 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5973096f8..c0c462008 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -74,6 +74,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `salmon`
   - `fusioninspector`
   - `all` => This will automatically run all of the above tools
+- Updated all `wget` containers to `conda-forge::wget=1.21.4` [#655](https://github.com/nf-core/rnafusion/pull/655)
 
 ### Fixed
 
diff --git a/modules/local/gencode_download/main.nf b/modules/local/gencode_download/main.nf
index a3308e827..569c8df1e 100644
--- a/modules/local/gencode_download/main.nf
+++ b/modules/local/gencode_download/main.nf
@@ -31,7 +31,7 @@ process GENCODE_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
     END_VERSIONS
     """
 
@@ -42,7 +42,7 @@ process GENCODE_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
     END_VERSIONS
     """
 
diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 0b4e4a2f5..576e4800a 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -20,7 +20,7 @@ process HGNC_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
     END_VERSIONS
     """
 
@@ -31,7 +31,7 @@ process HGNC_DOWNLOAD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
     END_VERSIONS
     """
 
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index ada898bc8..6e04afd16 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -41,7 +41,7 @@ process STARFUSION_BUILD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
         STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
     END_VERSIONS
     """
@@ -133,7 +133,7 @@ process STARFUSION_BUILD {
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
         STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
     END_VERSIONS
     """
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index d58e52dae..8ab99e9b8 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -17,7 +17,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -41,6 +41,7 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
@@ -210,7 +211,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:41:41.407790326"
+        "timestamp": "2025-04-23T10:48:48.9071057"
     },
     "stub test stringtie": {
         "content": [
@@ -230,7 +231,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -350,7 +351,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:50:39.921183013"
+        "timestamp": "2025-04-23T10:49:24.923946194"
     },
     "stub test arriba": {
         "content": [
@@ -376,7 +377,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -491,7 +492,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:39:53.022836825"
+        "timestamp": "2025-04-23T10:47:08.02222829"
     },
     "stub test fusioninspector": {
         "content": [
@@ -529,7 +530,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -538,7 +539,7 @@
                     "gtfToGenePred": 377
                 },
                 "HGNC_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
@@ -553,6 +554,7 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
@@ -864,7 +866,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-11T09:17:30.682830486"
+        "timestamp": "2025-04-23T10:51:45.508140056"
     },
     "stub test salmon": {
         "content": [
@@ -880,7 +882,7 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -955,7 +957,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:44:11.204966248"
+        "timestamp": "2025-04-23T10:51:03.157975722"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -981,7 +983,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1002,6 +1004,7 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
@@ -1184,7 +1187,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T16:59:53.744205313"
+        "timestamp": "2025-04-23T10:47:44.413622274"
     },
     "stub test fusionreport": {
         "content": [
@@ -1223,7 +1226,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1247,6 +1250,7 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
@@ -1445,7 +1449,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-16T10:39:10.08713305"
+        "timestamp": "2025-04-23T10:50:05.389865001"
     },
     "stub test fastp": {
         "content": [
@@ -1467,7 +1471,7 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1519,7 +1523,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-03T15:43:33.740487484"
+        "timestamp": "2025-04-23T10:50:34.188288253"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1542,7 +1546,7 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1592,7 +1596,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-16T10:35:22.7562139"
+        "timestamp": "2025-04-23T10:48:13.754663354"
     },
     "stub test all": {
         "content": [
@@ -1649,7 +1653,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1661,7 +1665,7 @@
                     "gtfToGenePred": 377
                 },
                 "HGNC_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
@@ -1685,6 +1689,7 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
@@ -2069,6 +2074,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-16T10:32:08.583950433"
+        "timestamp": "2025-04-23T10:46:29.001694439"
     }
 }
\ No newline at end of file

From 3260a04347baa1d15b1bebef3265aaedc05e0f3d Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 23 Apr 2025 11:36:00 +0000
Subject: [PATCH 458/887] fix snaps

---
 tests/test_build.nf.test.snap    |  5 ++--
 tests/test_stub_bam.nf.test.snap | 49 ++++++++++++++++++--------------
 2 files changed, 30 insertions(+), 24 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 2d07ed660..b59e6e189 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -25,7 +25,7 @@
                     "gtfToGenePred": 377
                 },
                 "HGNC_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "SALMON_INDEX": {
                     "salmon": "1.10.3"
@@ -34,6 +34,7 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -279,6 +280,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-03-19T14:46:22.709176112"
+        "timestamp": "2025-04-23T10:08:10.68706064"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index ac8414578..64d3b67a7 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -17,7 +17,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -38,6 +38,7 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -183,7 +184,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:09:48.999599917"
+        "timestamp": "2025-04-23T11:16:16.851201431"
     },
     "stub test stringtie": {
         "content": [
@@ -203,7 +204,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -299,7 +300,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:10:23.893097743"
+        "timestamp": "2025-04-23T11:16:59.945308111"
     },
     "stub test arriba": {
         "content": [
@@ -325,7 +326,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -413,7 +414,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:07:56.264754749"
+        "timestamp": "2025-04-23T11:14:09.827324661"
     },
     "stub test fusioninspector": {
         "content": [
@@ -451,7 +452,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -460,7 +461,7 @@
                     "gtfToGenePred": 377
                 },
                 "HGNC_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
@@ -472,6 +473,7 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -762,7 +764,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-11T09:25:46.143222996"
+        "timestamp": "2025-04-23T11:20:00.302967276"
     },
     "stub test salmon": {
         "content": [
@@ -782,7 +784,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -872,7 +874,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:12:30.758487268"
+        "timestamp": "2025-04-23T11:19:13.383274003"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -898,7 +900,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -916,6 +918,7 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -1077,7 +1080,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:08:38.341565744"
+        "timestamp": "2025-04-23T11:14:53.085896038"
     },
     "stub test fusionreport": {
         "content": [
@@ -1116,7 +1119,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1137,6 +1140,7 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -1311,7 +1315,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-16T10:51:50.320939135"
+        "timestamp": "2025-04-23T11:17:44.757543251"
     },
     "stub test fastp": {
         "content": [
@@ -1337,7 +1341,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1404,7 +1408,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-10T10:11:47.209265436"
+        "timestamp": "2025-04-23T11:18:29.048528226"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1431,7 +1435,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1496,7 +1500,7 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-16T10:48:04.746178504"
+        "timestamp": "2025-04-23T11:15:34.335857758"
     },
     "stub test all": {
         "content": [
@@ -1553,7 +1557,7 @@
                     "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
@@ -1565,7 +1569,7 @@
                     "gtfToGenePred": 377
                 },
                 "HGNC_DOWNLOAD": {
-                    "wget": null
+                    "wget": "1.21.4"
                 },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
@@ -1586,6 +1590,7 @@
                     "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
                     "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
@@ -1949,6 +1954,6 @@
             "nf-test": "0.9.2",
             "nextflow": "24.10.5"
         },
-        "timestamp": "2025-04-16T10:44:36.932311827"
+        "timestamp": "2025-04-23T11:13:24.397441064"
     }
 }
\ No newline at end of file

From ebd19b8902d1925a35cf8a032cc95debb2771b28 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 14:53:14 +0200
Subject: [PATCH 459/887] update starfusion build arguments

---
 CHANGELOG.md                              | 2 +-
 conf/modules.config                       | 1 +
 modules/local/fusioncatcher/build/main.nf | 0
 3 files changed, 2 insertions(+), 1 deletion(-)
 create mode 100644 modules/local/fusioncatcher/build/main.nf

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c0c462008..d84192716 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -118,7 +118,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Changed
 
-- Update to nf-tools 2.11.1 [#457] (https://github.com/nf-core/rnafusion/pull/457)
+- Update to nf-tools 2.11.1 [#457](https://github.com/nf-core/rnafusion/pull/457)
 - Update picard collectrnaseqmetrics memory requirements to 0.8x what is provided [#474](https://github.com/nf-core/rnafusion/pull/474)
 
 ### Fixed
diff --git a/conf/modules.config b/conf/modules.config
index 6314217e2..a67604af5 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -275,6 +275,7 @@ process {
     }
 
     withName: 'STARFUSION_BUILD' {
+        ext.args = { params.species.contains("homo_sapiens") ? "--human_gencode_filter": '' }
         cpus   = { 24    * task.attempt }
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }
diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
new file mode 100644
index 000000000..e69de29bb

From c54a51adcaf2cb996b260b4d43646d3aba0c2c47 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 14:55:27 +0200
Subject: [PATCH 460/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d84192716..5ad487b15 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -40,6 +40,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `splice_junctions` A file containing the splice junctions determined by STAR (needed by `ctatsplicing`)
 - Added `--fusioncatcher_download_link`. [#650](https://github.com/nf-core/rnafusion/pull/650)
 - Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
+- Using option `--human_gencode_filter` while building STARFusion references for human specie [#657](https://github.com/nf-core/rnafusion/pull/657)
 
 ### Changed
 

From 8c13f8c88cda5807becae43a205aaf939a33b983 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 15:22:33 +0200
Subject: [PATCH 461/887] Update CHANGELOG.md

Co-authored-by: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5ad487b15..964adfd2f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -40,7 +40,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `splice_junctions` A file containing the splice junctions determined by STAR (needed by `ctatsplicing`)
 - Added `--fusioncatcher_download_link`. [#650](https://github.com/nf-core/rnafusion/pull/650)
 - Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
-- Using option `--human_gencode_filter` while building STARFusion references for human specie [#657](https://github.com/nf-core/rnafusion/pull/657)
+- Using option `--human_gencode_filter` while building STARFusion references for human species [#657](https://github.com/nf-core/rnafusion/pull/657)
 
 ### Changed
 

From 53cfc831296163e948d177358efa9fcaef9b794c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 15:23:25 +0200
Subject: [PATCH 462/887] remove file

---
 modules/local/fusioncatcher/build/main.nf | 0
 1 file changed, 0 insertions(+), 0 deletions(-)
 delete mode 100644 modules/local/fusioncatcher/build/main.nf

diff --git a/modules/local/fusioncatcher/build/main.nf b/modules/local/fusioncatcher/build/main.nf
deleted file mode 100644
index e69de29bb..000000000

From 13ad9f15fd0dc2b32e0ffb4a104b3557bfab0ce4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 15:26:45 +0200
Subject: [PATCH 463/887] remove human_genocode_filter for tests

---
 tests/nextflow.config | 4 ++++
 1 file changed, 4 insertions(+)

diff --git a/tests/nextflow.config b/tests/nextflow.config
index abb1ba9c1..b1db55ff5 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -5,4 +5,8 @@ process {
         memory: '15.GB',
         time: '4.h'
     ]
+
+    withName: 'STARFUSION_BUILD' {
+        ext.args = ''
+    }
 }

From bd7113ad52f693c657fb41236f3d2d7a005ac9f4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 15:30:39 +0200
Subject: [PATCH 464/887] fusioncatcher test has to be re-addressed

---
 .../fusioncatcher_workflow/tests/main.nf.test | 66 -------------------
 .../tests/main.nf.test.snap                   | 37 -----------
 2 files changed, 103 deletions(-)
 delete mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap

diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
deleted file mode 100644
index ef6cecc7d..000000000
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ /dev/null
@@ -1,66 +0,0 @@
-nextflow_workflow {
-
-    name "Test Subworkflow FUSIONCATCHER_WORKFLOW"
-    script "../main.nf"
-    workflow "FUSIONCATCHER_WORKFLOW"
-    tag "subworkflow"
-    tag "fusioncatcher"
-    tag "fusioncatcher/build"
-    tag "fusioncatcher/detect"
-
-    // Test
-    test("FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4") {
-
-        setup {
-            // Download reference files for fusioncatch
-            run("FUSIONCATCHER_BUILD") {
-                script "../../../../modules/local/fusioncatcher/build/main.nf"
-                process {
-                """
-                input[0] = Channel.value('46')
-                """
-                }
-            }
-        }
-
-        // TODO: get smaller reference files for fusioncatcher
-        when {
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [ id: "test_fastqs", single_end: false ],
-                        [
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
-                        ]
-                    ] )
-
-                // ch_references
-                input[1] = FUSIONCATCHER_BUILD.out.reference
-
-                // fusioncatcher (boolean)
-                input[2] = true
-
-                // all (boolean)
-                input[3] = true
-
-                // fusioninspector_only (boolean)
-                input[4] = false
-
-                // fusioncatcher_fusions (string path)
-                input[5] = null
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(workflow.out).match() }
-            )
-        }
-    }
-
-}
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 47ffc5af1..000000000
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,37 +0,0 @@
-{
-    "FUSIONCATCHER_WORKFLOW - Homo sapiens - FASTQs chr4": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test_fastqs",
-                            "single_end": false
-                        },
-                        "test_fastqs.fusioncatcher.fusion-genes.txt:md5,c826a24c49abfcec8164c478e1e74892"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,05bd93a243728a293211ce52e5f97282"
-                ],
-                "fusions": [
-                    [
-                        {
-                            "id": "test_fastqs",
-                            "single_end": false
-                        },
-                        "test_fastqs.fusioncatcher.fusion-genes.txt:md5,c826a24c49abfcec8164c478e1e74892"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,05bd93a243728a293211ce52e5f97282"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2025-01-03T19:29:54.767628"
-    }
-}
\ No newline at end of file

From 61f19ef7170ba871e10e6c349fffe7ad69504c44 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 15:42:46 +0200
Subject: [PATCH 465/887] add handling of strandedness for picard
 collectrnaseqmetrics

---
 conf/modules.config | 15 +++++++++++++++
 1 file changed, 15 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 6314217e2..cbc568a1b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -188,6 +188,21 @@ process {
         ]
     }
 
+
+    withName:PICARD_COLLECTRNASEQMETRICS {
+        ext.args = { ( meta.strandedness == "forward" || meta.single_end ) ?
+            "--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND" :
+            meta.strandedness == "reverse" ?
+            "--STRAND_SPECIFICITY SECOND_READ_TRANSCRIPTION_STRAND" :
+            "--STRAND_SPECIFICITY NONE"
+        }
+        publishDir = [
+            path: { "${params.outdir}/picard" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
+        ]
+    }
+
     withName: 'SALMON_INDEX' {
         publishDir = [
             path: { "${params.genomes_base}/salmon" },

From e4ce78f8b86c37f7e756e4293770e6aca8b903af Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Apr 2025 15:46:52 +0200
Subject: [PATCH 466/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c0c462008..18a2a3cfd 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -40,6 +40,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `splice_junctions` A file containing the splice junctions determined by STAR (needed by `ctatsplicing`)
 - Added `--fusioncatcher_download_link`. [#650](https://github.com/nf-core/rnafusion/pull/650)
 - Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
+- Moved strandedness determination for picard collectrnaseqmetrics into modules.config [#658](https://github.com/nf-core/rnafusion/pull/658)
 
 ### Changed
 

From 683a29935180415449f77207e71f29083abb02f2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Apr 2025 11:08:52 +0200
Subject: [PATCH 467/887] modify build

---
 modules/local/starfusion/build/main.nf | 6 ++++--
 nextflow_schema.json                   | 2 +-
 2 files changed, 5 insertions(+), 3 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 6e04afd16..759cfebd1 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -21,14 +21,15 @@ process STARFUSION_BUILD {
     script:
     def args = task.ext.args ?: ''
     """
-    wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam34.0/Pfam-A.hmm.gz --no-check-certificate
-
+    wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3f --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3i --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3m --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3p --no-check-certificate
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
+    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
+
 
     prep_genome_lib.pl \\
         --genome_fa $fasta \\
@@ -36,6 +37,7 @@ process STARFUSION_BUILD {
         --dfam_db ${dfam_species}_dfam.hmm \\
         --pfam_db Pfam-A.hmm \\
         --fusion_annot_lib $fusion_annot_lib \\
+        --annot_filter_rule AnnotFilterRule.pm \\
         --CPU $task.cpus \\
         ${args}
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 190b98292..101e82c68 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -363,7 +363,7 @@
                 },
                 "dfam_version": {
                     "type": "string",
-                    "default": "3.4",
+                    "default": "3.9",
                     "description": "Version of dfam to use"
                 }
             }

From 0fc2aa3d30a931590355c962e24ba1132023f824 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Apr 2025 11:28:43 +0200
Subject: [PATCH 468/887] update dfam

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index fa731713b..0c1ba2735 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -32,7 +32,7 @@ params {
     genomes                    = [:]
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
     species                    = "homo_sapiens"
-    dfam_version               = '3.4'
+    dfam_version               = '3.9'
 
     // Filtering
     tools_cutoff               = 1

From 9f6fcb3527db1f5177064f61292d6b6521c89303 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Apr 2025 11:40:55 +0200
Subject: [PATCH 469/887] update dfam

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 0c1ba2735..fdbd7ccb1 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -32,7 +32,7 @@ params {
     genomes                    = [:]
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
     species                    = "homo_sapiens"
-    dfam_version               = '3.9'
+    dfam_version               = '3.8'
 
     // Filtering
     tools_cutoff               = 1

From 3da3c2f2f95e32f21a40fd689aad2588ac561770 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Sun, 27 Apr 2025 13:49:58 +0200
Subject: [PATCH 470/887] remove human filtering

---
 conf/modules.config | 1 -
 1 file changed, 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index a97d0806c..cbc568a1b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -290,7 +290,6 @@ process {
     }
 
     withName: 'STARFUSION_BUILD' {
-        ext.args = { params.species.contains("homo_sapiens") ? "--human_gencode_filter": '' }
         cpus   = { 24    * task.attempt }
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }

From aa7326415cb5848f9c5cf6775ca2f85bb0c830cc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Apr 2025 20:40:27 +0200
Subject: [PATCH 471/887] add filter again

---
 conf/modules.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/modules.config b/conf/modules.config
index cbc568a1b..a97d0806c 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -290,6 +290,7 @@ process {
     }
 
     withName: 'STARFUSION_BUILD' {
+        ext.args = { params.species.contains("homo_sapiens") ? "--human_gencode_filter": '' }
         cpus   = { 24    * task.attempt }
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }

From 3b71f2e90c45c465037e356ac1a815f7d1294363 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 29 Apr 2025 14:44:48 +0200
Subject: [PATCH 472/887] update bin path

---
 modules/local/starfusion/build/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 759cfebd1..57ac49de7 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -31,7 +31,7 @@ process STARFUSION_BUILD {
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
 
 
-    prep_genome_lib.pl \\
+    /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --dfam_db ${dfam_species}_dfam.hmm \\

From aee02d7336c432d98a4b95275294a7340e6de382 Mon Sep 17 00:00:00 2001
From: Rob Syme <rob.syme@gmail.com>
Date: Tue, 29 Apr 2025 08:59:40 -0400
Subject: [PATCH 473/887] Update fastq path regex to allow spaces in directory
 paths.

---
 assets/schema_input.json | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/assets/schema_input.json b/assets/schema_input.json
index 83684a04a..fe867086c 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -17,15 +17,15 @@
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^\\S+f(ast)?q\\.gz$",
-                "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces, has to exist and must have extension '.fq.gz' or '.fastq.gz'"
+                "pattern": "^([\\S\\s]*\\/)?[^\\s\\/]+\\.f(ast)?q\\.gz$",
+                "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces in filename, has to exist and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^\\S+f(ast)?q\\.gz$",
-                "errorMessage": "FastQ file for reads 2 cannot contain spaces, has to exist and must have extension '.fq.gz' or '.fastq.gz'"
+                "pattern": "^([\\S\\s]*\\/)?[^\\s\\/]+\\.f(ast)?q\\.gz$",
+                "errorMessage": "FastQ file for reads 2 cannot contain spaces in filename, has to exist and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "bam": {
                 "type": "string",

From 29e2f0806d468a60c35d4d39ccc07cc46e1a9378 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 30 Apr 2025 12:45:13 +0200
Subject: [PATCH 474/887] fix a bug in salmon index parsing

---
 subworkflows/local/build_references/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 30a26db36..c9729ed64 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -116,7 +116,7 @@ workflow BUILD_REFERENCES {
                 ch_versions = ch_versions.mix(SALMON_INDEX.out.versions)
                 ch_salmon_index = SALMON_INDEX.out.index
             } else {
-                ch_salmon_index = Channel.fromPath(params.salmon_index).map { it -> [[id:it.name], it] }
+                ch_salmon_index = Channel.fromPath(params.salmon_index)
             }
         }
     }

From 1aa8ff1afbcac749c21989c1ef2d7f98cbd11356 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Wed, 30 Apr 2025 12:28:11 +0000
Subject: [PATCH 475/887] Template update for nf-core/tools version 3.2.1

---
 .github/workflows/awsfulltest.yml | 41 ++++++++-----------------------
 .github/workflows/ci.yml          |  1 +
 .nf-core.yml                      |  2 +-
 nextflow.config                   |  2 +-
 ro-crate-metadata.json            | 36 +++++++++++++--------------
 5 files changed, 31 insertions(+), 51 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 2281992a2..a733b9c77 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -4,44 +4,23 @@ name: nf-core AWS full size tests
 # It runs the -profile 'test_full' on AWS batch
 
 on:
-  pull_request:
-    branches:
-      - main
-      - master
   workflow_dispatch:
   pull_request_review:
     types: [submitted]
+  release:
+    types: [published]
 
 jobs:
   run-platform:
     name: Run AWS full tests
-    # run only if the PR is approved by at least 2 reviewers and against the master branch or manually triggered
-    if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch'
+    # run only if the PR is approved by at least 2 reviewers and against the master/main branch or manually triggered
+    if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved' && (github.event.pull_request.base.ref == 'master' || github.event.pull_request.base.ref == 'main') || github.event_name == 'workflow_dispatch'
     runs-on: ubuntu-latest
     steps:
-      - name: Get PR reviews
-        uses: octokit/request-action@v2.x
-        if: github.event_name != 'workflow_dispatch'
-        id: check_approvals
-        continue-on-error: true
-        with:
-          route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews?per_page=100
-        env:
-          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-
-      - name: Check for approvals
-        if: ${{ failure() && github.event_name != 'workflow_dispatch' }}
-        run: |
-          echo "No review approvals found. At least 2 approvals are required to run this action automatically."
-          exit 1
-
-      - name: Check for enough approvals (>=2)
-        id: test_variables
-        if: github.event_name != 'workflow_dispatch'
+      - name: Set revision variable
+        id: revision
         run: |
-          JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}'
-          CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length')
-          test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required
+          echo "revision=${{ (github.event_name == 'workflow_dispatch' || github.event_name == 'release') && github.sha || 'dev' }}" >> "$GITHUB_OUTPUT"
 
       - name: Launch workflow via Seqera Platform
         uses: seqeralabs/action-tower-launch@v2
@@ -52,12 +31,12 @@ jobs:
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
           compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
-          revision: ${{ github.sha }}
-          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ github.sha }}
+          revision: ${{ steps.revision.outputs.revision }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/rnafusion/work-${{ steps.revision.outputs.revision }}
           parameters: |
             {
               "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}",
-              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}"
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ steps.revision.outputs.revision }}"
             }
           profiles: test_full
 
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 78fb3b574..59b0d7c77 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -83,5 +83,6 @@ jobs:
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
       - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
+        continue-on-error: ${{ matrix.NXF_VER == 'latest-everything' }}
         run: |
           nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} --outdir ./results
diff --git a/.nf-core.yml b/.nf-core.yml
index dae5ba31d..4431e715a 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,7 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
-nf_core_version: 3.2.0
+nf_core_version: 3.2.1
 repository_type: pipeline
 template:
   author: Martin Proks, Annick Renevey
diff --git a/nextflow.config b/nextflow.config
index a309f87e6..0322d3a80 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -257,7 +257,7 @@ manifest {
 
 // Nextflow plugins
 plugins {
-    id 'nf-schema@2.3.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+    id 'nf-schema@2.2.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 
 validation {
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 972b1a807..ccf7d6388 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,7 +22,7 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
-            "datePublished": "2025-01-27T14:47:28+00:00",
+            "datePublished": "2025-04-30T12:27:54+00:00",
             "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#472b4f7d-c1ad-4a49-9ebc-abac03e60cd0"
+                    "@id": "#2c818c4f-fbf7-42fa-9200-2b8dc9844428"
                 }
             ],
             "name": "nf-core/rnafusion"
@@ -124,20 +124,20 @@
             "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
             "creator": [
                 {
-                    "@id": "#phil.ewels@scilifelab.se"
+                    "@id": "https://orcid.org/0000-0001-9017-591X"
                 },
                 {
-                    "@id": "#max.u.garcia@gmail.com"
+                    "@id": "#phil.ewels@scilifelab.se"
                 },
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
                 },
                 {
-                    "@id": "https://orcid.org/0000-0001-9017-591X"
+                    "@id": "#max.u.garcia@gmail.com"
                 }
             ],
             "dateCreated": "",
-            "dateModified": "2025-01-27T14:47:28Z",
+            "dateModified": "2025-04-30T12:27:54Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
             "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
             "license": ["MIT"],
@@ -169,11 +169,11 @@
             "version": "!>=24.04.2"
         },
         {
-            "@id": "#472b4f7d-c1ad-4a49-9ebc-abac03e60cd0",
+            "@id": "#2c818c4f-fbf7-42fa-9200-2b8dc9844428",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#a52cf27d-3699-475d-b4c8-2eb08a2f0245"
+                    "@id": "#c6d68ba1-a887-413a-b175-e9947384e9d7"
                 }
             ],
             "mainEntity": {
@@ -182,7 +182,7 @@
             "name": "Test suite for nf-core/rnafusion"
         },
         {
-            "@id": "#a52cf27d-3699-475d-b4c8-2eb08a2f0245",
+            "@id": "#c6d68ba1-a887-413a-b175-e9947384e9d7",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/rnafusion",
             "resource": "repos/nf-core/rnafusion/actions/workflows/ci.yml",
@@ -311,16 +311,16 @@
             "url": "https://nf-co.re/"
         },
         {
-            "@id": "#phil.ewels@scilifelab.se",
+            "@id": "https://orcid.org/0000-0001-9017-591X",
             "@type": "Person",
-            "email": "phil.ewels@scilifelab.se",
-            "name": "Phil Ewels"
+            "email": "rickard.hammaren@scilifelab.se",
+            "name": "Rickard Hammar\u00e9n"
         },
         {
-            "@id": "#max.u.garcia@gmail.com",
+            "@id": "#phil.ewels@scilifelab.se",
             "@type": "Person",
-            "email": "max.u.garcia@gmail.com",
-            "name": "Maxime Garcia"
+            "email": "phil.ewels@scilifelab.se",
+            "name": "Phil Ewels"
         },
         {
             "@id": "https://orcid.org/0000-0002-8178-3128",
@@ -329,10 +329,10 @@
             "name": "Martin Proks"
         },
         {
-            "@id": "https://orcid.org/0000-0001-9017-591X",
+            "@id": "#max.u.garcia@gmail.com",
             "@type": "Person",
-            "email": "rickard.hammaren@scilifelab.se",
-            "name": "Rickard Hammar\u00e9n"
+            "email": "max.u.garcia@gmail.com",
+            "name": "Maxime Garcia"
         }
     ]
 }

From c2ac863971945947d5093f9c289cd247f1a3d21e Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 30 Apr 2025 15:39:35 +0000
Subject: [PATCH 476/887] fix linting error

---
 .github/workflows/ci.yml | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 779e7ac80..ecbefe6f4 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -92,9 +92,6 @@ jobs:
       - name: Clean up Disk space
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
-        continue-on-error: ${{ matrix.NXF_VER == 'latest-everything' }}
-
       - uses: actions/setup-python@v4
         with:
           python-version: "3.11"
@@ -110,6 +107,7 @@ jobs:
           version: ${{ env.NFT_VER }}
 
       - name: Run Tests (${{matrix.NXF_VER}} | ${{matrix.test_profile}} | ${{matrix.compute_profile}})
+        continue-on-error: ${{ matrix.NXF_VER == 'latest-everything' }}
         run: |
           nf-test test \
               --ci \

From 61c668fd6dc65969d0eb37e4b402e10c2f4795da Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 5 May 2025 09:30:49 +0200
Subject: [PATCH 477/887] update fastqc

---
 modules.json                           | 2 +-
 modules/nf-core/fastqc/environment.yml | 2 ++
 modules/nf-core/fastqc/main.nf         | 2 +-
 3 files changed, 4 insertions(+), 2 deletions(-)

diff --git a/modules.json b/modules.json
index 04fcccc52..151ea80e6 100644
--- a/modules.json
+++ b/modules.json
@@ -37,7 +37,7 @@
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
+                        "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
                         "installed_by": ["modules"]
                     },
                     "fusioncatcher/fusioncatcher": {
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
index 691d4c763..f9f54ee9b 100644
--- a/modules/nf-core/fastqc/environment.yml
+++ b/modules/nf-core/fastqc/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 033f4154a..23e16634c 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -29,7 +29,7 @@ process FASTQC {
     // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
     // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
     // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
-    def memory_in_mb = task.memory ? task.memory.toUnit('MB').toFloat() / task.cpus : null
+    def memory_in_mb = task.memory ? task.memory.toUnit('MB') / task.cpus : null
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
 

From aa29fe99831cfd8accf9ca07e5a6ff6b0bba2616 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 5 May 2025 09:53:58 +0200
Subject: [PATCH 478/887] update fastp

---
 modules.json                                  |   2 +-
 modules/nf-core/fastp/environment.yml         |   4 +-
 modules/nf-core/fastp/main.nf                 |   4 +-
 modules/nf-core/fastp/meta.yml                |   5 +-
 modules/nf-core/fastp/tests/main.nf.test.snap | 180 +++++++++---------
 modules/nf-core/fastp/tests/tags.yml          |   2 -
 6 files changed, 99 insertions(+), 98 deletions(-)
 delete mode 100644 modules/nf-core/fastp/tests/tags.yml

diff --git a/modules.json b/modules.json
index 151ea80e6..e6453690e 100644
--- a/modules.json
+++ b/modules.json
@@ -32,7 +32,7 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
+                        "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/fastp/environment.yml
index 26d4aca5d..90adcd2c5 100644
--- a/modules/nf-core/fastp/environment.yml
+++ b/modules/nf-core/fastp/environment.yml
@@ -1,5 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fastp=0.23.4
+  - bioconda::fastp=0.24.0
diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf
index e1b9f5656..1342741d5 100644
--- a/modules/nf-core/fastp/main.nf
+++ b/modules/nf-core/fastp/main.nf
@@ -4,8 +4,8 @@ process FASTP {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fastp:0.23.4--h5f740d0_0' :
-        'biocontainers/fastp:0.23.4--h5f740d0_0' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/88/889a182b8066804f4799f3808a5813ad601381a8a0e3baa4ab8d73e739b97001/data' :
+        'community.wave.seqera.io/library/fastp:0.24.0--62c97b06e8447690' }"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml
index 159404d08..9c4b24584 100644
--- a/modules/nf-core/fastp/meta.yml
+++ b/modules/nf-core/fastp/meta.yml
@@ -30,8 +30,9 @@ input:
         pattern: "*.{fasta,fna,fas,fa}"
   - - discard_trimmed_pass:
         type: boolean
-        description: Specify true to not write any reads that pass trimming thresholds.
-          | This can be used to use fastp for the output report only.
+        description: |
+          Specify true to not write any reads that pass trimming thresholds.
+          This can be used to use fastp for the output report only.
   - - save_trimmed_fail:
         type: boolean
         description: Specify true to save files that failed to pass trimming thresholds
diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap
index 54be7e45f..9e2aaf315 100644
--- a/modules/nf-core/fastp/tests/main.nf.test.snap
+++ b/modules/nf-core/fastp/tests/main.nf.test.snap
@@ -39,7 +39,7 @@
                     
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -78,15 +78,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T14:31:10.841098"
+        "timestamp": "2025-03-31T13:40:51.8619133"
     },
     "test_fastp_paired_end": {
         "content": [
@@ -96,7 +96,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd"
+                    "test.fastp.json:md5,7cf3bff1922b512bcca58439eb2d3679"
                 ]
             ],
             [
@@ -118,14 +118,14 @@
                 
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:43:28.665779"
+        "timestamp": "2025-03-31T13:39:15.121411815"
     },
     "test_fastp_paired_end_merged_adapterlist": {
         "content": [
@@ -135,7 +135,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.fastp.json:md5,5914ca3f21ce162123a824e33e8564f6"
+                    "test.fastp.json:md5,533983f8c11cc3f2ccdcea01531f68ae"
                 ]
             ],
             [
@@ -163,14 +163,14 @@
                 ]
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:44:18.210375"
+        "timestamp": "2025-03-31T13:39:51.561598206"
     },
     "test_fastp_single_end_qc_only": {
         "content": [
@@ -180,7 +180,7 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.fastp.json:md5,5cc5f01e449309e0e689ed6f51a2294a"
+                    "test.fastp.json:md5,93f407199ee8b94c023c291bddfc4dce"
                 ]
             ],
             [
@@ -202,14 +202,14 @@
                 
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:44:27.380974"
+        "timestamp": "2025-03-31T13:39:56.392912049"
     },
     "test_fastp_paired_end_trim_fail": {
         "content": [
@@ -247,18 +247,18 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.fastp.json:md5,4c3268ddb50ea5b33125984776aa3519"
+                    "test.fastp.json:md5,5009a892192f2084c2af69c153d88d6c"
                 ]
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:43:58.749589"
+        "timestamp": "2025-03-31T13:39:38.690242568"
     },
     "fastp - stub test_fastp_interleaved": {
         "content": [
@@ -306,7 +306,7 @@
                     
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -351,15 +351,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:50:00.270029"
+        "timestamp": "2025-03-31T13:40:23.678200076"
     },
     "test_fastp_single_end - stub": {
         "content": [
@@ -407,7 +407,7 @@
                     
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -452,15 +452,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:49:42.502789"
+        "timestamp": "2025-03-31T13:40:08.756547678"
     },
     "test_fastp_paired_end_merged_adapterlist - stub": {
         "content": [
@@ -517,7 +517,7 @@
                     ]
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -571,15 +571,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:54:53.458252"
+        "timestamp": "2025-03-31T13:40:47.596331823"
     },
     "test_fastp_paired_end_merged - stub": {
         "content": [
@@ -636,7 +636,7 @@
                     ]
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -690,15 +690,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:50:27.689379"
+        "timestamp": "2025-03-31T13:40:39.962303534"
     },
     "test_fastp_paired_end_merged": {
         "content": [
@@ -708,7 +708,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.fastp.json:md5,b712fd68ed0322f4bec49ff2a5237fcc"
+                    "test.fastp.json:md5,8f99097bfa04b629891105b8af9c429f"
                 ]
             ],
             [
@@ -736,14 +736,14 @@
                 ]
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:44:08.68476"
+        "timestamp": "2025-03-31T13:39:46.300238743"
     },
     "test_fastp_paired_end - stub": {
         "content": [
@@ -794,7 +794,7 @@
                     
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -842,15 +842,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:49:51.679221"
+        "timestamp": "2025-03-31T13:40:16.309925689"
     },
     "test_fastp_single_end": {
         "content": [
@@ -860,7 +860,7 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc"
+                    "test.fastp.json:md5,81dc86dd695967bb5c015e0a978bf20c"
                 ]
             ],
             [
@@ -879,14 +879,14 @@
                 
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:43:18.834322"
+        "timestamp": "2025-03-31T13:39:07.133909607"
     },
     "test_fastp_single_end_trim_fail - stub": {
         "content": [
@@ -940,7 +940,7 @@
                     
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -991,15 +991,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T14:05:36.898142"
+        "timestamp": "2025-03-31T13:40:28.086573661"
     },
     "test_fastp_paired_end_trim_fail - stub": {
         "content": [
@@ -1060,7 +1060,7 @@
                     
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -1118,15 +1118,15 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T14:05:49.212847"
+        "timestamp": "2025-03-31T13:40:35.606162029"
     },
     "fastp test_fastp_interleaved": {
         "content": [
@@ -1145,18 +1145,18 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.fastp.json:md5,b24e0624df5cc0b11cd5ba21b726fb22"
+                    "test.fastp.json:md5,101003b8ac634ca5fd381656ac2b8b9f"
                 ]
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:43:38.910832"
+        "timestamp": "2025-03-31T13:39:23.114582408"
     },
     "test_fastp_single_end_trim_fail": {
         "content": [
@@ -1166,7 +1166,7 @@
                         "id": "test",
                         "single_end": true
                     },
-                    "test.fastp.json:md5,9a7ee180f000e8d00c7fb67f06293eb5"
+                    "test.fastp.json:md5,d721f75d68a382c819b6499e6325a942"
                 ]
             ],
             [
@@ -1191,14 +1191,14 @@
                 
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:43:48.22378"
+        "timestamp": "2025-03-31T13:39:30.757538842"
     },
     "test_fastp_paired_end_qc_only": {
         "content": [
@@ -1208,7 +1208,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.fastp.json:md5,623064a45912dac6f2b64e3f2e9901df"
+                    "test.fastp.json:md5,1ad31d6559ff5d4d275f501f3f5c02b9"
                 ]
             ],
             [
@@ -1230,14 +1230,14 @@
                 
             ],
             [
-                "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T13:44:36.334938"
+        "timestamp": "2025-03-31T13:40:01.381972575"
     },
     "test_fastp_paired_end_qc_only - stub": {
         "content": [
@@ -1279,7 +1279,7 @@
                     
                 ],
                 "6": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ],
                 "html": [
                     [
@@ -1318,14 +1318,14 @@
                     
                 ],
                 "versions": [
-                    "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02"
+                    "versions.yml:md5,3799377c4173131ba9392346e1c40bb9"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2024-07-05T14:31:27.096468"
+        "timestamp": "2025-03-31T13:40:56.392034947"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/fastp/tests/tags.yml b/modules/nf-core/fastp/tests/tags.yml
deleted file mode 100644
index c1afcce75..000000000
--- a/modules/nf-core/fastp/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-fastp:
-  - modules/nf-core/fastp/**

From 1f421d768ecae0fa9794561adb011c0aba996589 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 08:50:38 +0200
Subject: [PATCH 479/887] update starfusion

---
 modules/local/starfusion/build/environment.yml | 4 ++--
 modules/local/starfusion/build/main.nf         | 4 ++--
 modules/local/starfusion/detect/main.nf        | 4 ++--
 3 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index ef7f93160..8ad0c17bf 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -4,5 +4,5 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.28
-  - bioconda::star-fusion=1.14.0
+  - bioconda::minimap2=2.29
+  - bioconda::star-fusion=1.15.0
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 57ac49de7..ecd8a71a8 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -4,8 +4,8 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 00c78efc7..0bf271541 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,8 +4,8 @@ process STARFUSION {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/be/bed86145102fdf7e381e1a506a4723676f98b4bbe1db5085d02213cef18525c9/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:aa3a8e3951498552'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
 
     input:
     tuple val(meta), path(reads), path(junction)

From f6dc429383b4368bfe6240d56002e0ed2ec6b367 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 08:57:50 +0200
Subject: [PATCH 480/887] dfam_version to 3.8 only

---
 nextflow_schema.json | 1 -
 1 file changed, 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 101e82c68..be565ef1f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -363,7 +363,6 @@
                 },
                 "dfam_version": {
                     "type": "string",
-                    "default": "3.9",
                     "description": "Version of dfam to use"
                 }
             }

From d6c3a470f5110d1d2f1da6caae1953cd22a98e56 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 10:15:49 +0200
Subject: [PATCH 481/887] update fusionreport

---
 modules/local/fusionreport/detect/environment.yml   | 2 +-
 modules/local/fusionreport/detect/main.nf           | 4 ++--
 modules/local/fusionreport/download/environment.yml | 2 +-
 modules/local/fusionreport/download/main.nf         | 4 ++--
 4 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/modules/local/fusionreport/detect/environment.yml b/modules/local/fusionreport/detect/environment.yml
index 0d260fdb8..b3d78e480 100644
--- a/modules/local/fusionreport/detect/environment.yml
+++ b/modules/local/fusionreport/detect/environment.yml
@@ -2,5 +2,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fusion-report=3.1.1
+  - bioconda::fusion-report=4.0.0
   - conda-forge::openpyxl=3.1.5
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index bcb59877c..f912fcdb7 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -4,8 +4,8 @@ process FUSIONREPORT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d9d1075dc45da6b08ec99c6e8bcc83e0ab71a674e7efdc7a36e459539793fcf9/data' :
-        'community.wave.seqera.io/library/fusion-report_openpyxl:6748677442b83a9a'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/df/df38cfae9eebc99efdcd10782598f0d56320f17b66d173714e5fc99638ad88f3/data' :
+        'community.wave.seqera.io/library/fusion-report:4.0.0--9d994e2b7da545ce'}"
 
 
     input:
diff --git a/modules/local/fusionreport/download/environment.yml b/modules/local/fusionreport/download/environment.yml
index 0d260fdb8..b3d78e480 100644
--- a/modules/local/fusionreport/download/environment.yml
+++ b/modules/local/fusionreport/download/environment.yml
@@ -2,5 +2,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fusion-report=3.1.1
+  - bioconda::fusion-report=4.0.0
   - conda-forge::openpyxl=3.1.5
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index ec62c75eb..fe3f3df7d 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -4,8 +4,8 @@ process FUSIONREPORT_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d9d1075dc45da6b08ec99c6e8bcc83e0ab71a674e7efdc7a36e459539793fcf9/data' :
-        'community.wave.seqera.io/library/fusion-report_openpyxl:6748677442b83a9a'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/df/df38cfae9eebc99efdcd10782598f0d56320f17b66d173714e5fc99638ad88f3/data' :
+        'community.wave.seqera.io/library/fusion-report:4.0.0--9d994e2b7da545ce'}"
 
     output:
     tuple val(meta), path("fusion_report_db"), emit: fusionreport_ref

From 03231fd23bc9b7bcf10140e5eebc3dc40bea5607 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 11:44:59 +0200
Subject: [PATCH 482/887] add args2

---
 modules/local/fusionreport/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index fe3f3df7d..460963024 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -16,7 +16,7 @@ process FUSIONREPORT_DOWNLOAD {
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
     """
-    fusion_report download $args ./
+    fusion_report download $args $args2 ./
     mkdir fusion_report_db
     mv *.txt *.log *.db fusion_report_db/
 

From b6ddefe71256f7f7b21f59bc770008b28983ae16 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 12:10:04 +0200
Subject: [PATCH 483/887] fix untriggered condition

---
 conf/modules.config | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index a97d0806c..c9d614eb1 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -65,7 +65,7 @@ process {
     }
 
     withName: 'FASTP' {
-        ext.args = params.trim_tail ? "--trim_tail1 ${params.trim_tail} --trim_tail2 ${params.trim_tail} " : ''
+        ext.args = { params.trim_tail ? "--trim_tail1 ${params.trim_tail} --trim_tail2 ${params.trim_tail} " : '' }
     }
 
     withName: 'FASTQC' {
@@ -104,12 +104,12 @@ process {
     }
 
     withName: '.*FUSIONINSPECTOR_WORKFLOW:.*:FUSIONINSPECTOR' {
-        ext.args = { ${params.fusioninspector_limitSjdbInsertNsj} != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
+        ext.args = { params.fusioninspector_limitSjdbInsertNsj != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
         ext.args2 = '--annotate --examine_coding_effect'
     }
 
     withName: 'FUSIONREPORT' {
-        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : "" }
+        ext.args         = { params.no_cosmic ? "--no-cosmic" : "" }
         ext.args2        = "--export csv"
         publishDir = [
             path: { "${params.outdir}/fusionreport/${meta.id}" },
@@ -119,7 +119,7 @@ process {
     }
 
     withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : " --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
+        ext.args         = { params.no_cosmic ? "--no-cosmic" : " --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         ext.args2        = { params.qiagen ? "--qiagen" : "" }
         publishDir = [
             path: { "${params.genomes_base}" },
@@ -171,7 +171,7 @@ process {
     }
 
     withName: 'MULTIQC' {
-        ext.args   = {params.multiqc_title} ? "--title \"$params.multiqc_title\"" : ''
+        ext.args   = {params.multiqc_title ? "--title \"$params.multiqc_title\"" : ''}
         publishDir = [
             path: { "${params.outdir}/multiqc" },
             mode: params.publish_dir_mode,

From 6b0900562df5f4e4e7140ad940ef45c901a97bb5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 12:39:39 +0200
Subject: [PATCH 484/887] update containers

---
 modules/local/fusionreport/detect/main.nf   | 4 ++--
 modules/local/fusionreport/download/main.nf | 4 ++--
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusionreport/detect/main.nf b/modules/local/fusionreport/detect/main.nf
index f912fcdb7..5143d53c8 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/local/fusionreport/detect/main.nf
@@ -4,8 +4,8 @@ process FUSIONREPORT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/df/df38cfae9eebc99efdcd10782598f0d56320f17b66d173714e5fc99638ad88f3/data' :
-        'community.wave.seqera.io/library/fusion-report:4.0.0--9d994e2b7da545ce'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d99b7576d14caafd0494d6e2c1453edd161a933ccb62940701074128d3718bc5/data' :
+        'community.wave.seqera.io/library/fusion-report_openpyxl:77a2ba7e76ae0694'}"
 
 
     input:
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 460963024..9c19a64fc 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -4,8 +4,8 @@ process FUSIONREPORT_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/df/df38cfae9eebc99efdcd10782598f0d56320f17b66d173714e5fc99638ad88f3/data' :
-        'community.wave.seqera.io/library/fusion-report:4.0.0--9d994e2b7da545ce'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d99b7576d14caafd0494d6e2c1453edd161a933ccb62940701074128d3718bc5/data'' :
+        'community.wave.seqera.io/library/fusion-report_openpyxl:77a2ba7e76ae0694'}"
 
     output:
     tuple val(meta), path("fusion_report_db"), emit: fusionreport_ref

From 85a1031ded7d40d7be8bae3e348eb30c36a9d532 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 13:27:20 +0200
Subject: [PATCH 485/887] update container

---
 modules/local/fusionreport/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 9c19a64fc..3b9072c3d 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -4,7 +4,7 @@ process FUSIONREPORT_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d99b7576d14caafd0494d6e2c1453edd161a933ccb62940701074128d3718bc5/data'' :
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/71/71ff06a821ba96a2c6b75af8d2b9aff969756b90af2d04a9a5ea1e9c60bb4be0/data'' :
         'community.wave.seqera.io/library/fusion-report_openpyxl:77a2ba7e76ae0694'}"
 
     output:

From 52bc57c74d2529fe0a0ac51fbcd2d24ba49dfec6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 14:00:26 +0200
Subject: [PATCH 486/887] update container

---
 modules/local/fusionreport/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 3b9072c3d..125c0d2fb 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -4,7 +4,7 @@ process FUSIONREPORT_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/71/71ff06a821ba96a2c6b75af8d2b9aff969756b90af2d04a9a5ea1e9c60bb4be0/data'' :
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d99b7576d14caafd0494d6e2c1453edd161a933ccb62940701074128d3718bc5/data' :
         'community.wave.seqera.io/library/fusion-report_openpyxl:77a2ba7e76ae0694'}"
 
     output:

From 111d20b174e9a2f66d3767dc75149637b2c31905 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 14:50:22 +0200
Subject: [PATCH 487/887] update config

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index c9d614eb1..edfac8a0e 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -119,7 +119,7 @@ process {
     }
 
     withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args         = { params.no_cosmic ? "--no-cosmic" : " --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
+        ext.args         = { params.no_cosmic ? "--no-cosmic" : "--cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         ext.args2        = { params.qiagen ? "--qiagen" : "" }
         publishDir = [
             path: { "${params.genomes_base}" },

From f8ef0c0cec0988f20ca670de1091fef21f533a24 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 6 May 2025 15:02:01 +0200
Subject: [PATCH 488/887] reshuffle arguments

---
 conf/modules.config                         | 3 +--
 modules/local/fusionreport/download/main.nf | 3 +--
 2 files changed, 2 insertions(+), 4 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index edfac8a0e..4bc495eb9 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -119,8 +119,7 @@ process {
     }
 
     withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args         = { params.no_cosmic ? "--no-cosmic" : "--cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
-        ext.args2        = { params.qiagen ? "--qiagen" : "" }
+        ext.args         = { params.no_cosmic ? "--no-cosmic" : params.qiagen ? "--qiagen --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" : "--cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         publishDir = [
             path: { "${params.genomes_base}" },
             mode: params.publish_dir_mode,
diff --git a/modules/local/fusionreport/download/main.nf b/modules/local/fusionreport/download/main.nf
index 125c0d2fb..839c20151 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/local/fusionreport/download/main.nf
@@ -14,9 +14,8 @@ process FUSIONREPORT_DOWNLOAD {
     script:
     meta = [id: 'fusion_report_db']
     def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
     """
-    fusion_report download $args $args2 ./
+    fusion_report download $args ./
     mkdir fusion_report_db
     mv *.txt *.log *.db fusion_report_db/
 

From 93dc8e15e96555b58ced22f6992ee7b828ec39a8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 09:34:38 +0200
Subject: [PATCH 489/887] update fusionreport version

---
 tests/test_build.nf.test.snap    |  4 ++--
 tests/test_stub.nf.test.snap     | 14 +++++++-------
 tests/test_stub_bam.nf.test.snap | 14 +++++++-------
 3 files changed, 16 insertions(+), 16 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index b59e6e189..5c5d08924 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -7,7 +7,7 @@
                     "arriba_download": "2.4.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -282,4 +282,4 @@
         },
         "timestamp": "2025-04-23T10:08:10.68706064"
     }
-}
\ No newline at end of file
+}
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 8ab99e9b8..7495d02c4 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -514,10 +514,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1210,10 +1210,10 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1637,10 +1637,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -2076,4 +2076,4 @@
         },
         "timestamp": "2025-04-23T10:46:29.001694439"
     }
-}
\ No newline at end of file
+}
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 64d3b67a7..99b8e5e31 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -436,10 +436,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1103,10 +1103,10 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1541,10 +1541,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1956,4 +1956,4 @@
         },
         "timestamp": "2025-04-23T11:13:24.397441064"
     }
-}
\ No newline at end of file
+}

From fed55fb2d77dd782726f89d7557de8db52a1b51e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 10:00:02 +0200
Subject: [PATCH 490/887] update cli call

---
 modules/local/starfusion/build/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index ecd8a71a8..0d7ab3230 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -31,7 +31,7 @@ process STARFUSION_BUILD {
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
 
 
-    /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl \\
+    prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --dfam_db ${dfam_species}_dfam.hmm \\

From b82b302fa042b01619f704baaa2946c995bc5048 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 10:59:05 +0200
Subject: [PATCH 491/887] update stub bam snaps

---
 tests/test_stub_bam.nf.test.snap | 56 ++++++++++++++++----------------
 1 file changed, 28 insertions(+), 28 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 64d3b67a7..c84fb94ab 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -35,11 +35,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -181,10 +181,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:16:16.851201431"
+        "timestamp": "2025-05-07T10:45:30.913775592"
     },
     "stub test stringtie": {
         "content": [
@@ -474,7 +474,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -761,10 +761,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:20:00.302967276"
+        "timestamp": "2025-05-07T10:47:54.208994663"
     },
     "stub test salmon": {
         "content": [
@@ -919,7 +919,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -1077,10 +1077,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:14:53.085896038"
+        "timestamp": "2025-05-07T10:44:30.208342353"
     },
     "stub test fusionreport": {
         "content": [
@@ -1137,11 +1137,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -1312,17 +1312,17 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:17:44.757543251"
+        "timestamp": "2025-05-07T10:46:25.615179804"
     },
     "stub test fastp": {
         "content": [
             13,
             {
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1405,10 +1405,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:18:29.048528226"
+        "timestamp": "2025-05-07T10:46:52.127766054"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1522,7 +1522,7 @@
                     "ctat-splicing": "0.0.2"
                 },
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1587,11 +1587,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -1951,9 +1951,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:13:24.397441064"
+        "timestamp": "2025-05-07T10:43:25.04013859"
     }
 }
\ No newline at end of file

From 8ad7def56fc957ef5fa7e693c3fa40cf29c30899 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 11:48:47 +0200
Subject: [PATCH 492/887] update snap

---
 tests/test_build.nf.test.snap | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index b59e6e189..db01143fb 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -35,7 +35,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -220,7 +220,7 @@
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
-                "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+                "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -277,9 +277,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:08:10.68706064"
+        "timestamp": "2025-05-07T11:43:30.893014622"
     }
 }
\ No newline at end of file

From e1a5295819c5a43cc194ae1fe0918a6b0c4ae4ce Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 13:18:52 +0200
Subject: [PATCH 493/887] update modules, remove unused modules

---
 CHANGELOG.md                                  |   1 +
 modules.json                                  | 179 +++---
 .../agat/convertspgff2tsv/environment.yml     |   4 +-
 modules/nf-core/agat/convertspgff2tsv/main.nf |   5 +-
 .../convertspgff2tsv/tests/main.nf.test.snap  |  18 +-
 .../agat/convertspgff2tsv/tests/tags.yml      |   2 -
 .../nf-core/arriba/download/environment.yml   |   2 +
 modules/nf-core/arriba/download/meta.yml      |   4 +-
 .../arriba/download/tests/main.nf.test.snap   |   8 +-
 .../nf-core/arriba/download/tests/tags.yml    |   2 -
 modules/nf-core/cat/cat/environment.yml       |   2 +
 modules/nf-core/cat/cat/tests/tags.yml        |   2 -
 modules/nf-core/cat/fastq/environment.yml     |   7 +
 modules/nf-core/cat/fastq/main.nf             |  31 +-
 modules/nf-core/cat/fastq/tests/main.nf.test  |  90 ++-
 .../nf-core/cat/fastq/tests/main.nf.test.snap | 176 +++---
 modules/nf-core/cat/fastq/tests/tags.yml      |   2 -
 .../gatk4/bedtointervallist/environment.yml   |   1 -
 .../gatk4/bedtointervallist/tests/tags.yml    |   2 -
 .../createsequencedictionary/environment.yml  |   1 -
 .../createsequencedictionary/tests/tags.yml   |   2 -
 .../gatk4/markduplicates/environment.yml      |   7 +-
 modules/nf-core/gatk4/markduplicates/main.nf  |  35 +-
 .../markduplicates/tests/main.nf.test.snap    |  56 +-
 .../gatk4/markduplicates/tests/tags.yml       |   2 -
 modules/nf-core/gffread/environment.yml       |   2 +
 modules/nf-core/gffread/tests/tags.yml        |   2 -
 modules/nf-core/multiqc/environment.yml       |   4 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  18 +-
 modules/nf-core/multiqc/tests/tags.yml        |   2 -
 .../collectinsertsizemetrics/environment.yml  |   2 +
 .../picard/collectinsertsizemetrics/main.nf   |   2 -
 .../collectrnaseqmetrics/environment.yml      |   2 +
 .../picard/collectwgsmetrics/environment.yml  |   2 +
 .../nf-core/rrnatranscripts/environment.yml   |   7 -
 modules/nf-core/rrnatranscripts/main.nf       |  43 --
 modules/nf-core/rrnatranscripts/meta.yml      |  34 --
 .../templates/get_rrna_transcripts.py         |  83 ---
 .../rrnatranscripts/tests/main.nf.test        |  53 --
 .../rrnatranscripts/tests/main.nf.test.snap   |  40 --
 .../nf-core/rrnatranscripts/tests/tags.yml    |   2 -
 modules/nf-core/salmon/index/environment.yml  |   2 +
 modules/nf-core/salmon/index/main.nf          |  27 +-
 .../nf-core/salmon/index/tests/main.nf.test   |  39 +-
 .../salmon/index/tests/main.nf.test.snap      |  31 +-
 modules/nf-core/salmon/index/tests/tags.yml   |   2 -
 modules/nf-core/salmon/quant/main.nf          |  91 +--
 modules/nf-core/salmon/quant/tests/tags.yml   |   2 -
 .../nf-core/samtools/convert/tests/tags.yml   |   2 -
 modules/nf-core/samtools/faidx/main.nf        |  13 +-
 modules/nf-core/samtools/faidx/meta.yml       |  20 +-
 .../nf-core/samtools/faidx/tests/main.nf.test | 107 +++-
 .../samtools/faidx/tests/main.nf.test.snap    | 336 ++++++++++-
 modules/nf-core/samtools/faidx/tests/tags.yml |   2 -
 modules/nf-core/samtools/index/tests/tags.yml |   2 -
 modules/nf-core/samtools/sort/tests/tags.yml  |   3 -
 modules/nf-core/samtools/view/environment.yml |  10 -
 modules/nf-core/samtools/view/main.nf         |  77 ---
 modules/nf-core/samtools/view/meta.yml        | 141 -----
 .../nf-core/samtools/view/tests/bam.config    |   3 -
 .../samtools/view/tests/bam_index.config      |   3 -
 .../nf-core/samtools/view/tests/main.nf.test  | 214 -------
 .../samtools/view/tests/main.nf.test.snap     | 528 ------------------
 modules/nf-core/samtools/view/tests/tags.yml  |   2 -
 modules/nf-core/star/align/tests/tags.yml     |   2 -
 .../star/genomegenerate/environment.yml       |   6 +-
 modules/nf-core/star/genomegenerate/main.nf   |   4 +-
 .../genomegenerate/tests/main.nf.test.snap    |  36 +-
 .../star/genomegenerate/tests/tags.yml        |   2 -
 .../nf-core/stringtie/merge/environment.yml   |   2 +
 .../nf-core/stringtie/merge/tests/tags.yml    |   2 -
 .../stringtie/stringtie/environment.yml       |   2 +
 .../stringtie/stringtie/tests/tags.yml        |   2 -
 .../ucsc/gtftogenepred/environment.yml        |   2 +
 subworkflows/local/build_references/main.nf   |   2 +-
 .../starfusion_workflow/tests/nextflow.config |   4 -
 77 files changed, 1040 insertions(+), 1626 deletions(-)
 delete mode 100644 modules/nf-core/agat/convertspgff2tsv/tests/tags.yml
 delete mode 100644 modules/nf-core/arriba/download/tests/tags.yml
 delete mode 100644 modules/nf-core/cat/cat/tests/tags.yml
 delete mode 100644 modules/nf-core/cat/fastq/tests/tags.yml
 delete mode 100644 modules/nf-core/gatk4/bedtointervallist/tests/tags.yml
 delete mode 100644 modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml
 delete mode 100644 modules/nf-core/gatk4/markduplicates/tests/tags.yml
 delete mode 100644 modules/nf-core/gffread/tests/tags.yml
 delete mode 100644 modules/nf-core/multiqc/tests/tags.yml
 delete mode 100644 modules/nf-core/rrnatranscripts/environment.yml
 delete mode 100644 modules/nf-core/rrnatranscripts/main.nf
 delete mode 100644 modules/nf-core/rrnatranscripts/meta.yml
 delete mode 100644 modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
 delete mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test
 delete mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/rrnatranscripts/tests/tags.yml
 delete mode 100644 modules/nf-core/salmon/index/tests/tags.yml
 delete mode 100644 modules/nf-core/salmon/quant/tests/tags.yml
 delete mode 100644 modules/nf-core/samtools/convert/tests/tags.yml
 delete mode 100644 modules/nf-core/samtools/faidx/tests/tags.yml
 delete mode 100644 modules/nf-core/samtools/index/tests/tags.yml
 delete mode 100644 modules/nf-core/samtools/sort/tests/tags.yml
 delete mode 100644 modules/nf-core/samtools/view/environment.yml
 delete mode 100644 modules/nf-core/samtools/view/main.nf
 delete mode 100644 modules/nf-core/samtools/view/meta.yml
 delete mode 100644 modules/nf-core/samtools/view/tests/bam.config
 delete mode 100644 modules/nf-core/samtools/view/tests/bam_index.config
 delete mode 100644 modules/nf-core/samtools/view/tests/main.nf.test
 delete mode 100644 modules/nf-core/samtools/view/tests/main.nf.test.snap
 delete mode 100644 modules/nf-core/samtools/view/tests/tags.yml
 delete mode 100644 modules/nf-core/star/align/tests/tags.yml
 delete mode 100644 modules/nf-core/star/genomegenerate/tests/tags.yml
 delete mode 100644 modules/nf-core/stringtie/merge/tests/tags.yml
 delete mode 100644 modules/nf-core/stringtie/stringtie/tests/tags.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 83516b786..34e3932c7 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -42,6 +42,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
 - Moved strandedness determination for picard collectrnaseqmetrics into modules.config [#658](https://github.com/nf-core/rnafusion/pull/658)
 - Using option `--human_gencode_filter` while building STARFusion references for human species [#657](https://github.com/nf-core/rnafusion/pull/657)
+- Update STAR-Fusion to 1.15.0 and using human filtering when species is human [#664](https://github.com/nf-core/rnafusion/pull/664)
 
 ### Changed
 
diff --git a/modules.json b/modules.json
index e6453690e..8bb54eef5 100644
--- a/modules.json
+++ b/modules.json
@@ -7,149 +7,192 @@
                 "nf-core": {
                     "agat/convertspgff2tsv": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "1719e20e3881b37b9e1e8498ea669a2b92e54b9a",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "467c202a876d26af544fa8c4b22a050a535462a7",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "a1abf90966a2a4016d3c3e41e228bfcbd4811ccc",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "2a227f75f33415f74a884ef8b33555b151e33de9",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
-                        "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
-                        "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
-                        "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
-                        "installed_by": ["modules"]
+                        "git_sha": "1ec937ab3edc307bc0d79a2200d784e9f0868359",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gffread": {
                         "branch": "master",
-                        "git_sha": "bd5f75ccaf2345269810e66e85de8a70e4de8764",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
-                        "installed_by": ["modules"]
+                        "git_sha": "7b50cb7be890e4b28cffb82e438cc6a8d7805d3f",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
-                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "git_sha": "980a4964be91da75fb3ccf576118a18e247f6d15",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
-                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
-                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
-                    },
-                    "rrnatranscripts": {
-                        "branch": "master",
-                        "git_sha": "812edf8cf702de42d2d8c7314d6f03b97e20abeb",
-                        "installed_by": ["modules"],
-                        "patch": "modules/nf-core/rrnatranscripts/rrnatranscripts.diff"
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/index": {
                         "branch": "master",
-                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/quant": {
                         "branch": "master",
-                        "git_sha": "85b5f8a0d9df9ce7587af50e2ee75b37c97515c6",
-                        "installed_by": ["modules"]
+                        "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/convert": {
                         "branch": "master",
-                        "git_sha": "b823b7220bac94079f9ea65113401956ca7d633e",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
-                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "b13f07be4c508d6ff6312d354d09f2493243e208",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "b7800db9b069ed505db3f9d91b8c72faea9be17b",
-                        "installed_by": ["modules"]
-                    },
-                    "samtools/view": {
-                        "branch": "master",
-                        "git_sha": "2d20463181b1c38981a02e90d3084b5f9fa8d540",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
-                        "git_sha": "a5ad53288c79fa52c5ae708c317e09ec2dd149ab",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "49f4e50534fe4b64101e62ea41d5dc43b1324358",
-                        "installed_by": ["modules"]
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
-                        "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
-                        "installed_by": ["modules"]
+                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -158,20 +201,26 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/agat/convertspgff2tsv/environment.yml b/modules/nf-core/agat/convertspgff2tsv/environment.yml
index 0410ee765..072565894 100644
--- a/modules/nf-core/agat/convertspgff2tsv/environment.yml
+++ b/modules/nf-core/agat/convertspgff2tsv/environment.yml
@@ -1,5 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::agat=1.4.0
+  - bioconda::agat=1.4.2
diff --git a/modules/nf-core/agat/convertspgff2tsv/main.nf b/modules/nf-core/agat/convertspgff2tsv/main.nf
index a6f73b6c1..772c7bd0b 100644
--- a/modules/nf-core/agat/convertspgff2tsv/main.nf
+++ b/modules/nf-core/agat/convertspgff2tsv/main.nf
@@ -4,8 +4,8 @@ process AGAT_CONVERTSPGFF2TSV {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/agat:1.4.0--pl5321hdfd78af_0' :
-        'biocontainers/agat:1.4.0--pl5321hdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/agat:1.4.2--pl5321hdfd78af_0' :
+        'biocontainers/agat:1.4.2--pl5321hdfd78af_0' }"
 
     input:
     tuple val(meta), path(gff)
@@ -33,7 +33,6 @@ process AGAT_CONVERTSPGFF2TSV {
     """
 
     stub:
-    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.tsv
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
index 71ed6205c..50a428d62 100644
--- a/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
+++ b/modules/nf-core/agat/convertspgff2tsv/tests/main.nf.test.snap
@@ -3,14 +3,14 @@
         "content": [
             [
                 "test.tsv",
-                "versions.yml:md5,b81565a6ff8911848806128b3bec8508"
+                "versions.yml:md5,1866660991c9d87acc28236b9d026438"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-05-08T10:06:55.853319"
+        "timestamp": "2025-01-15T13:13:51.099041"
     },
     "sarscov2 - genome [gff3]": {
         "content": [
@@ -24,7 +24,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,b81565a6ff8911848806128b3bec8508"
+                    "versions.yml:md5,1866660991c9d87acc28236b9d026438"
                 ],
                 "tsv": [
                     [
@@ -35,14 +35,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,b81565a6ff8911848806128b3bec8508"
+                    "versions.yml:md5,1866660991c9d87acc28236b9d026438"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
+            "nf-test": "0.9.0",
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-05-08T10:06:51.415395"
+        "timestamp": "2025-01-15T13:13:44.904263"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/agat/convertspgff2tsv/tests/tags.yml b/modules/nf-core/agat/convertspgff2tsv/tests/tags.yml
deleted file mode 100644
index d2ee37213..000000000
--- a/modules/nf-core/agat/convertspgff2tsv/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-agat/convertspgff2tsv:
-  - "modules/nf-core/agat/convertspgff2tsv/**"
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
index d0883a0d2..e8f5b278f 100644
--- a/modules/nf-core/arriba/download/environment.yml
+++ b/modules/nf-core/arriba/download/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
index bdf542ebc..d9e28cc06 100644
--- a/modules/nf-core/arriba/download/meta.yml
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -36,13 +36,13 @@ output:
       - protein_domains*${genome}*.gff3:
           type: file
           description: Protein domain annotations
-          patter: "*.gff3"
+          pattern: "*.gff3"
   - known_fusions:
       - known_fusions*${genome}*.tsv.gz:
           type: file
           description: Arriba is more sensitive to those fusions to improve the detection
             rate of expected or highly relevant events, such as recurrent fusions
-          patter: "*.tsv.gz"
+          pattern: "*.tsv.gz"
   - versions:
       - versions.yml:
           type: file
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
index fe9b18b94..d12a38bbf 100644
--- a/modules/nf-core/arriba/download/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/download/tests/main.nf.test.snap
@@ -1,5 +1,5 @@
 {
-    "download": {
+    "test-arriba-download": {
         "content": [
             {
                 "0": [
@@ -35,9 +35,9 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-08T11:12:17.010496"
+        "timestamp": "2024-12-16T01:46:32.110653034"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/arriba/download/tests/tags.yml b/modules/nf-core/arriba/download/tests/tags.yml
deleted file mode 100644
index f510bbf1a..000000000
--- a/modules/nf-core/arriba/download/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-arriba/download:
-  - "modules/nf-core/arriba/download/**"
diff --git a/modules/nf-core/cat/cat/environment.yml b/modules/nf-core/cat/cat/environment.yml
index 9b01c865a..50c2059af 100644
--- a/modules/nf-core/cat/cat/environment.yml
+++ b/modules/nf-core/cat/cat/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/cat/cat/tests/tags.yml b/modules/nf-core/cat/cat/tests/tags.yml
deleted file mode 100644
index 37b578f52..000000000
--- a/modules/nf-core/cat/cat/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-cat/cat:
-  - modules/nf-core/cat/cat/**
diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml
index 71e04c3d7..9b926b1ff 100644
--- a/modules/nf-core/cat/fastq/environment.yml
+++ b/modules/nf-core/cat/fastq/environment.yml
@@ -1,5 +1,12 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
   - conda-forge::coreutils=9.5
+  - conda-forge::grep=3.11
+  - conda-forge::gzip=1.13
+  - conda-forge::lbzip2=2.5
+  - conda-forge::sed=4.8
+  - conda-forge::tar=1.34
diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf
index 4364a389b..acfb6d0e6 100644
--- a/modules/nf-core/cat/fastq/main.nf
+++ b/modules/nf-core/cat/fastq/main.nf
@@ -1,26 +1,25 @@
 process CAT_FASTQ {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c2/c262fc09eca59edb5a724080eeceb00fb06396f510aefb229c2d2c6897e63975/data' :
-        'community.wave.seqera.io/library/coreutils:9.5--ae99c88a9b28c264' }"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/52/52ccce28d2ab928ab862e25aae26314d69c8e38bd41ca9431c67ef05221348aa/data'
+        : 'community.wave.seqera.io/library/coreutils_grep_gzip_lbzip2_pruned:838ba80435a629f8'}"
 
     input:
     tuple val(meta), path(reads, stageAs: "input*/*")
 
     output:
     tuple val(meta), path("*.merged.fastq.gz"), emit: reads
-    path "versions.yml"                       , emit: versions
+    path "versions.yml", emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def readList = reads instanceof List ? reads.collect{ it.toString() } : [reads.toString()]
+    def readList = reads instanceof List ? reads.collect { it.toString() } : [reads.toString()]
     if (meta.single_end) {
         if (readList.size >= 1) {
             """
@@ -31,12 +30,15 @@ process CAT_FASTQ {
                 cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
             END_VERSIONS
             """
+        } else {
+            error("Could not find any FASTQ files to concatenate in the process input")
         }
-    } else {
+    }
+    else {
         if (readList.size >= 2) {
             def read1 = []
             def read2 = []
-            readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v }
+            readList.eachWithIndex { v, ix -> (ix & 1 ? read2 : read1) << v }
             """
             cat ${read1.join(' ')} > ${prefix}_1.merged.fastq.gz
             cat ${read2.join(' ')} > ${prefix}_2.merged.fastq.gz
@@ -46,12 +48,14 @@ process CAT_FASTQ {
                 cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
             END_VERSIONS
             """
+        } else {
+            error("Could not find any FASTQ file pairs to concatenate in the process input")
         }
     }
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def readList = reads instanceof List ? reads.collect{ it.toString() } : [reads.toString()]
+    def readList = reads instanceof List ? reads.collect { it.toString() } : [reads.toString()]
     if (meta.single_end) {
         if (readList.size >= 1) {
             """
@@ -62,8 +66,11 @@ process CAT_FASTQ {
                 cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
             END_VERSIONS
             """
+        } else {
+            error("Could not find any FASTQ files to concatenate in the process input")
         }
-    } else {
+    }
+    else {
         if (readList.size >= 2) {
             """
             echo '' | gzip > ${prefix}_1.merged.fastq.gz
@@ -74,6 +81,8 @@ process CAT_FASTQ {
                 cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
             END_VERSIONS
             """
+        } else {
+            error("Could not find any FASTQ file pairs to concatenate in the process input")
         }
     }
 }
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test b/modules/nf-core/cat/fastq/tests/main.nf.test
index f88a78b6c..013c1d0f4 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test
@@ -1,5 +1,3 @@
-// NOTE The version snaps may not be consistant
-// https://github.com/nf-core/modules/pull/4087#issuecomment-1767948035
 nextflow_process {
 
     name "Test Process CAT_FASTQ"
@@ -245,4 +243,92 @@ nextflow_process {
             )
         }
     }
+
+    test("test_cat_fastq_single_end_no_files") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    []
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.failed },
+                { assert snapshot(process.stdout.find { it.contains("-- Check script") }.split(" -- Check script")[0]).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_paired_end_no_files") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    []
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.failed },
+                { assert snapshot(process.stdout.find { it.contains("-- Check script") }.split(" -- Check script")[0]).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_single_end_no_files - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    []
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.failed },
+                { assert snapshot(process.stdout.find { it.contains("-- Check script") }.split(" -- Check script")[0]).match() }
+            )
+        }
+    }
+
+    test("test_cat_fastq_paired_end_no_files - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    []
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.failed },
+                { assert snapshot(process.stdout.find { it.contains("-- Check script") }.split(" -- Check script")[0]).match() }
+            )
+        }
+    }
 }
diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test.snap b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
index f8689a1ce..ee5ab3647 100644
--- a/modules/nf-core/cat/fastq/tests/main.nf.test.snap
+++ b/modules/nf-core/cat/fastq/tests/main.nf.test.snap
@@ -1,5 +1,15 @@
 {
-    "test_cat_fastq_single_end": {
+    "test_cat_fastq_paired_end_no_files - stub": {
+        "content": [
+            "  Could not find any FASTQ file pairs to concatenate in the process input"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
+        },
+        "timestamp": "2025-02-25T17:14:51.248685461"
+    },
+    "test_cat_fastq_single_end_single_file": {
         "content": [
             {
                 "0": [
@@ -8,7 +18,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
+                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
                     ]
                 ],
                 "1": [
@@ -20,7 +30,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
+                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
                     ]
                 ],
                 "versions": [
@@ -29,21 +39,24 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:02:07.519211144"
+        "timestamp": "2025-02-25T17:24:04.902821069"
     },
-    "test_cat_fastq_single_end_same_name": {
+    "test_cat_fastq_paired_end_same_name": {
         "content": [
             {
                 "0": [
                     [
                         {
                             "id": "test",
-                            "single_end": true
+                            "single_end": false
                         },
-                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
                     ]
                 ],
                 "1": [
@@ -53,9 +66,12 @@
                     [
                         {
                             "id": "test",
-                            "single_end": true
+                            "single_end": false
                         },
-                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
+                        [
+                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
+                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
+                        ]
                     ]
                 ],
                 "versions": [
@@ -64,21 +80,24 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:02:31.618628921"
+        "timestamp": "2025-02-25T17:23:57.476357974"
     },
-    "test_cat_fastq_single_end_single_file": {
+    "test_cat_fastq_paired_end_same_name - stub": {
         "content": [
             {
                 "0": [
                     [
                         {
                             "id": "test",
-                            "single_end": true
+                            "single_end": false
                         },
-                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                        [
+                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
                     ]
                 ],
                 "1": [
@@ -88,9 +107,12 @@
                     [
                         {
                             "id": "test",
-                            "single_end": true
+                            "single_end": false
                         },
-                        "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec"
+                        [
+                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
                     ]
                 ],
                 "versions": [
@@ -99,24 +121,21 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:02:57.904149581"
+        "timestamp": "2025-02-25T17:24:34.615815265"
     },
-    "test_cat_fastq_paired_end_same_name": {
+    "test_cat_fastq_single_end": {
         "content": [
             {
                 "0": [
                     [
                         {
                             "id": "test",
-                            "single_end": false
+                            "single_end": true
                         },
-                        [
-                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
-                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
-                        ]
+                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
                     ]
                 ],
                 "1": [
@@ -126,12 +145,9 @@
                     [
                         {
                             "id": "test",
-                            "single_end": false
+                            "single_end": true
                         },
-                        [
-                            "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22",
-                            "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5"
-                        ]
+                        "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da"
                     ]
                 ],
                 "versions": [
@@ -140,12 +156,12 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:02:44.577183829"
+        "timestamp": "2025-02-25T17:23:32.489874386"
     },
-    "test_cat_fastq_single_end - stub": {
+    "test_cat_fastq_single_end_same_name": {
         "content": [
             {
                 "0": [
@@ -154,7 +170,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
                     ]
                 ],
                 "1": [
@@ -166,7 +182,7 @@
                             "id": "test",
                             "single_end": true
                         },
-                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22"
                     ]
                 ],
                 "versions": [
@@ -175,24 +191,21 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:03:10.603734777"
+        "timestamp": "2025-02-25T17:23:49.184759506"
     },
-    "test_cat_fastq_paired_end_same_name - stub": {
+    "test_cat_fastq_single_end - stub": {
         "content": [
             {
                 "0": [
                     [
                         {
                             "id": "test",
-                            "single_end": false
+                            "single_end": true
                         },
-                        [
-                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                     ]
                 ],
                 "1": [
@@ -202,12 +215,9 @@
                     [
                         {
                             "id": "test",
-                            "single_end": false
+                            "single_end": true
                         },
-                        [
-                            "test_1.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "test_2.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
+                        "test.merged.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                     ]
                 ],
                 "versions": [
@@ -216,10 +226,30 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
+        },
+        "timestamp": "2025-02-25T17:24:12.857293744"
+    },
+    "test_cat_fastq_paired_end_no_files": {
+        "content": [
+            "  Could not find any FASTQ file pairs to concatenate in the process input"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
+        },
+        "timestamp": "2025-02-25T17:14:40.806088747"
+    },
+    "test_cat_fastq_single_end_no_files - stub": {
+        "content": [
+            "  Could not find any FASTQ files to concatenate in the process input"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:03:46.041808828"
+        "timestamp": "2025-02-25T17:14:45.852365218"
     },
     "test_cat_fastq_single_end_same_name - stub": {
         "content": [
@@ -251,10 +281,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:03:34.13865402"
+        "timestamp": "2025-02-25T17:24:27.816080065"
     },
     "test_cat_fastq_paired_end": {
         "content": [
@@ -292,10 +322,20 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
+        },
+        "timestamp": "2025-02-25T17:23:41.739469187"
+    },
+    "test_cat_fastq_single_end_no_files": {
+        "content": [
+            "  Could not find any FASTQ files to concatenate in the process input"
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:02:19.64383573"
+        "timestamp": "2025-02-25T17:14:35.695192409"
     },
     "test_cat_fastq_paired_end - stub": {
         "content": [
@@ -333,10 +373,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:03:22.597246066"
+        "timestamp": "2025-02-25T17:24:21.178950408"
     },
     "test_cat_fastq_single_end_single_file - stub": {
         "content": [
@@ -368,9 +408,9 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2024-10-19T20:03:58.44849001"
+        "timestamp": "2025-02-25T17:24:40.851404993"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/cat/fastq/tests/tags.yml b/modules/nf-core/cat/fastq/tests/tags.yml
deleted file mode 100644
index 6ac436140..000000000
--- a/modules/nf-core/cat/fastq/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-cat/fastq:
-  - modules/nf-core/cat/fastq/**
diff --git a/modules/nf-core/gatk4/bedtointervallist/environment.yml b/modules/nf-core/gatk4/bedtointervallist/environment.yml
index 1f7d08246..b562b72c7 100644
--- a/modules/nf-core/gatk4/bedtointervallist/environment.yml
+++ b/modules/nf-core/gatk4/bedtointervallist/environment.yml
@@ -6,5 +6,4 @@ channels:
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
   - bioconda::gatk4=4.6.1.0
-  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/bedtointervallist/tests/tags.yml b/modules/nf-core/gatk4/bedtointervallist/tests/tags.yml
deleted file mode 100644
index b4d54f129..000000000
--- a/modules/nf-core/gatk4/bedtointervallist/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-gatk4/bedtointervallist:
-  - "modules/nf-core/gatk4/bedtointervallist/**"
diff --git a/modules/nf-core/gatk4/createsequencedictionary/environment.yml b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
index 1f7d08246..b562b72c7 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/environment.yml
+++ b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
@@ -6,5 +6,4 @@ channels:
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
   - bioconda::gatk4=4.6.1.0
-  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml b/modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml
deleted file mode 100644
index 035c5e4c7..000000000
--- a/modules/nf-core/gatk4/createsequencedictionary/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-gatk4/createsequencedictionary:
-  - "modules/nf-core/gatk4/createsequencedictionary/**"
diff --git a/modules/nf-core/gatk4/markduplicates/environment.yml b/modules/nf-core/gatk4/markduplicates/environment.yml
index ec65c32da..352d20f83 100644
--- a/modules/nf-core/gatk4/markduplicates/environment.yml
+++ b/modules/nf-core/gatk4/markduplicates/environment.yml
@@ -7,9 +7,6 @@ channels:
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
   - bioconda::gatk4=4.6.1.0
-  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
-  # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.19.1
-  # renovate: datasource=conda depName=bioconda/samtools
-  - bioconda::samtools=1.19.2
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
\ No newline at end of file
diff --git a/modules/nf-core/gatk4/markduplicates/main.nf b/modules/nf-core/gatk4/markduplicates/main.nf
index cf770308d..f4bd896bf 100644
--- a/modules/nf-core/gatk4/markduplicates/main.nf
+++ b/modules/nf-core/gatk4/markduplicates/main.nf
@@ -1,24 +1,24 @@
 process GATK4_MARKDUPLICATES {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-d9e7bad0f7fbc8f4458d5c3ab7ffaaf0235b59fb:7cc3d06cbf42e28c5e2ebfc7c858654c7340a9d5-0':
-        'biocontainers/mulled-v2-d9e7bad0f7fbc8f4458d5c3ab7ffaaf0235b59fb:7cc3d06cbf42e28c5e2ebfc7c858654c7340a9d5-0' }"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/927ff9bb80d65b425cbe752db6648a84043feff6e8ca90e60f9ff6ddbe8938d5/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel_htslib_samtools:c1e4292d6ee27439'}"
 
     input:
     tuple val(meta), path(bam)
-    path  fasta
-    path  fasta_fai
+    path fasta
+    path fasta_fai
 
     output:
-    tuple val(meta), path("*cram"),     emit: cram,  optional: true
-    tuple val(meta), path("*bam"),      emit: bam,   optional: true
-    tuple val(meta), path("*.crai"),    emit: crai,  optional: true
-    tuple val(meta), path("*.bai"),     emit: bai,   optional: true
+    tuple val(meta), path("*cram"), emit: cram, optional: true
+    tuple val(meta), path("*bam"), emit: bam, optional: true
+    tuple val(meta), path("*.crai"), emit: crai, optional: true
+    tuple val(meta), path("*.bai"), emit: bai, optional: true
     tuple val(meta), path("*.metrics"), emit: metrics
-    path "versions.yml",                emit: versions
+    path "versions.yml", emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -30,14 +30,15 @@ process GATK4_MARKDUPLICATES {
     // If the extension is CRAM, then change it to BAM
     prefix_bam = prefix.tokenize('.')[-1] == 'cram' ? "${prefix.substring(0, prefix.lastIndexOf('.'))}.bam" : prefix
 
-    def input_list = bam.collect{"--INPUT $it"}.join(' ')
+    def input_list = bam.collect { "--INPUT ${it}" }.join(' ')
     def reference = fasta ? "--REFERENCE_SEQUENCE ${fasta}" : ""
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
 
     // Using samtools and not Markduplicates to compress to CRAM speeds up computation:
@@ -45,12 +46,12 @@ process GATK4_MARKDUPLICATES {
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         MarkDuplicates \\
-        $input_list \\
+        ${input_list} \\
         --OUTPUT ${prefix_bam} \\
         --METRICS_FILE ${prefix}.metrics \\
         --TMP_DIR . \\
         ${reference} \\
-        $args
+        ${args}
 
     # If cram files are wished as output, the run samtools for conversion
     if [[ ${prefix} == *.cram ]]; then
diff --git a/modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap b/modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap
index 336bb3735..227ce6985 100644
--- a/modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap
+++ b/modules/nf-core/gatk4/markduplicates/tests/main.nf.test.snap
@@ -7,15 +7,15 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.bam:md5,8a808b1a94d2627c4d659a2151c4cb9f"
+                    "test.bam:md5,d4c87a668273b589b59cf88b5a00bceb"
                 ]
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.03.1"
         },
-        "timestamp": "2024-02-13T15:21:36.059923"
+        "timestamp": "2025-05-06T19:13:35.646267615"
     },
     "multi crai": {
         "content": [
@@ -35,27 +35,27 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.bai:md5,38b99c5f771895ecf5324c3186b9d452"
+                    "test.bai:md5,2b1603773979ad06f4dbcbaa90e93e8c"
                 ]
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.03.1"
         },
-        "timestamp": "2024-02-13T15:21:36.09642"
+        "timestamp": "2025-05-06T19:13:35.773636892"
     },
     "versions": {
         "content": [
             [
-                "versions.yml:md5,c58bf16c6e3786cc4d17bb7249f9ffe5"
+                "versions.yml:md5,836be8dcb8e596b2e5eeb2d667db0832"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.03.1"
         },
-        "timestamp": "2024-02-13T15:21:08.710549"
+        "timestamp": "2025-05-06T19:37:41.921624379"
     },
     "multi test.metrics": {
         "content": [
@@ -75,39 +75,39 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.bai:md5,26001bcdbce12e9f07557d8f7b8d360e"
+                    "test.bai:md5,fd1c3cc02f4e223d32eedeed842b86c7"
                 ]
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.03.1"
         },
-        "timestamp": "2023-12-12T17:42:39.651888758"
+        "timestamp": "2025-05-06T19:13:21.104275114"
     },
     "multi cram versions": {
         "content": [
             [
-                "versions.yml:md5,c58bf16c6e3786cc4d17bb7249f9ffe5"
+                "versions.yml:md5,836be8dcb8e596b2e5eeb2d667db0832"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.03.1"
         },
-        "timestamp": "2024-02-13T15:21:56.966376"
+        "timestamp": "2025-05-06T19:38:08.855960769"
     },
     "multi versions": {
         "content": [
             [
-                "versions.yml:md5,c58bf16c6e3786cc4d17bb7249f9ffe5"
+                "versions.yml:md5,836be8dcb8e596b2e5eeb2d667db0832"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.03.1"
         },
-        "timestamp": "2024-02-13T15:21:36.138095"
+        "timestamp": "2025-05-06T19:37:55.051564286"
     },
     "multi cram test.metrics": {
         "content": [
@@ -137,15 +137,15 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.bam:md5,75d914ba8804eaf2acf02ab432197ec9"
+                    "test.bam:md5,5542c553af351a43b97e84181c51a84e"
                 ]
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.03.1"
         },
-        "timestamp": "2024-02-13T15:21:08.645892"
+        "timestamp": "2025-05-06T19:13:21.060566406"
     },
     "test.metrics": {
         "content": [
diff --git a/modules/nf-core/gatk4/markduplicates/tests/tags.yml b/modules/nf-core/gatk4/markduplicates/tests/tags.yml
deleted file mode 100644
index 8632e32b3..000000000
--- a/modules/nf-core/gatk4/markduplicates/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-gatk4/markduplicates:
-  - "modules/nf-core/gatk4/markduplicates/**"
diff --git a/modules/nf-core/gffread/environment.yml b/modules/nf-core/gffread/environment.yml
index ee2398416..46c5faece 100644
--- a/modules/nf-core/gffread/environment.yml
+++ b/modules/nf-core/gffread/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/gffread/tests/tags.yml b/modules/nf-core/gffread/tests/tags.yml
deleted file mode 100644
index 055760656..000000000
--- a/modules/nf-core/gffread/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-gffread:
-  - modules/nf-core/gffread/**
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index a27122ce1..d430da5f7 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -1,5 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.27
+  - bioconda::multiqc=1.28
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 58d9313c6..f3b570472 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.27--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.27--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.28--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.28--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index 7b7c13220..26d74307d 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,b05075d2d2b4f485c0d627a5c8e475b2"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-27T09:29:57.631982377"
+        "timestamp": "2025-03-26T16:05:18.927925"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,b05075d2d2b4f485c0d627a5c8e475b2"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-27T09:30:34.743726958"
+        "timestamp": "2025-03-26T16:05:55.639955"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,b05075d2d2b4f485c0d627a5c8e475b2"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "24.10.4"
         },
-        "timestamp": "2025-01-27T09:30:21.44383553"
+        "timestamp": "2025-03-26T16:05:44.067369"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml
deleted file mode 100644
index bea6c0d37..000000000
--- a/modules/nf-core/multiqc/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-multiqc:
-  - modules/nf-core/multiqc/**
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
index 1d715d564..8f34e975c 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
+++ b/modules/nf-core/picard/collectinsertsizemetrics/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/main.nf b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
index c3014d806..bed044028 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/main.nf
+++ b/modules/nf-core/picard/collectinsertsizemetrics/main.nf
@@ -32,7 +32,6 @@ process PICARD_COLLECTINSERTSIZEMETRICS {
     picard \\
         -Xmx${avail_mem}M \\
         CollectInsertSizeMetrics \\
-        $args \\
         --INPUT $bam \\
         --OUTPUT ${prefix}.txt \\
         --Histogram_FILE ${prefix}.pdf \\
@@ -47,7 +46,6 @@ process PICARD_COLLECTINSERTSIZEMETRICS {
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def avail_mem = 3072
     if (!task.memory) {
         log.info '[Picard CollectInsertSizeMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
     }
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
index 1d715d564..8f34e975c 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
+++ b/modules/nf-core/picard/collectrnaseqmetrics/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/picard/collectwgsmetrics/environment.yml b/modules/nf-core/picard/collectwgsmetrics/environment.yml
index 13265842f..5f2dfc031 100644
--- a/modules/nf-core/picard/collectwgsmetrics/environment.yml
+++ b/modules/nf-core/picard/collectwgsmetrics/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/rrnatranscripts/environment.yml b/modules/nf-core/rrnatranscripts/environment.yml
deleted file mode 100644
index 6f09494b4..000000000
--- a/modules/nf-core/rrnatranscripts/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - python=3.12.2
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
deleted file mode 100644
index 982c53cd4..000000000
--- a/modules/nf-core/rrnatranscripts/main.nf
+++ /dev/null
@@ -1,43 +0,0 @@
-process RRNATRANSCRIPTS {
-    tag "$gtf"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/python:3.12' :
-        'biocontainers/python:3.12' }"
-
-    input:
-    path(gtf)
-
-    output:
-    path("*rrna_intervals.gtf") , emit: rrna_gtf, optional: true
-    path "versions.yml"         , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def prefix = task.ext.prefix ?: "${gtf.baseName}"
-    """
-    grep -E '^#|rRNA' ${gtf} > ${prefix}_rrna_intervals.gtf || true
-    if [ !  -s ${prefix}_rrna_intervals.gtf ]; then
-        rm ${prefix}_rrna_intervals.gtf
-    fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        python: \$(python --version | sed -e "s/Python //g")
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${gtf.baseName}"
-    """
-    touch ${prefix}_rrna_intervals.gtf
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        python: \$(python --version | sed -e "s/Python //g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/rrnatranscripts/meta.yml b/modules/nf-core/rrnatranscripts/meta.yml
deleted file mode 100644
index 93f6a10ef..000000000
--- a/modules/nf-core/rrnatranscripts/meta.yml
+++ /dev/null
@@ -1,34 +0,0 @@
-name: rrnatranscripts
-description: Ribosomal RNA extraction from a GTF file.
-keywords:
-  - ribosomal
-  - rna
-  - genomics
-tools:
-  - rrnatranscripts:
-      description: |
-        Extraction of ribosomal RNA
-      homepage: https://github.com/nf-core/rnafusion
-      licence: ["GPL-3.0-or-later"]
-      identifier: ""
-input:
-  - - gtf:
-        type: file
-        description: GTF file
-        pattern: "*.{gtf}"
-output:
-  #
-  - rrna_gtf:
-      - "*rrna_intervals.gtf":
-          type: file
-          description: GTF file with ribosomal RNA only
-          pattern: "*.{gtf}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@rannick"
-maintainers:
-  - "@rannick"
diff --git a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
deleted file mode 100644
index ea3ce0f49..000000000
--- a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
+++ /dev/null
@@ -1,83 +0,0 @@
-#!/usr/bin/env python3
-
-import logging
-import platform
-import sys
-from pathlib import Path
-
-# Configure logging
-logging.basicConfig(format="%(name)s - %(asctime)s %(levelname)s: %(message)s")
-logger = logging.getLogger(__name__)
-logger.setLevel(logging.INFO)
-
-
-def get_rrna_intervals(gtf: str, rrna_transcripts: str):
-    """
-    Get lines containing ``#`` or ``gene_type rRNA`` or ```` or ``gene_type rRNA_pseudogene`` or ``gene_type MT_rRNA``
-    Create output file
-
-    Args:
-        file_in (pathlib.Path): The given GTF file.
-        file_out (pathlib.Path): Where the ribosomal RNA GTF file should
-            be created; always in GTF format.
-    """
-    patterns = {
-        "#",
-        'transcript_biotype "Mt_rRNA"',
-        'transcript_biotype "rRNA"',
-        'transcript_biotype "rRNA_pseudogene"',
-    }
-    line_starts = {"MT", "1", "2", "3", "4", "5", "6", "7", "8", "9"}
-    out_lines = []
-    path_gtf = Path(gtf)
-    path_rrna_transcripts = Path(rrna_transcripts)
-    if not path_gtf.is_file():
-        logger.error(f"The given input file {gtf} was not found!")
-        sys.exit(2)
-    with path_gtf.open() as f:
-        data = f.readlines()
-        for line in data:
-            for pattern in patterns:
-                if pattern in line:
-                    for line_start in line_starts:
-                        if line.startswith(line_start):
-                            out_lines.append(line)
-    if out_lines != []:
-        with path_rrna_transcripts.open(mode="w") as out_file:
-            out_file.writelines(out_lines)
-
-
-def format_yaml_like(data: dict, indent: int = 0) -> str:
-    """Formats a dictionary to a YAML-like string.
-
-    Args:
-        data (dict): The dictionary to format.
-        indent (int): The current indentation level.
-
-    Returns:
-        str: A string formatted as YAML.
-    """
-    yaml_str = ""
-    for key, value in data.items():
-        spaces = "  " * indent
-        if isinstance(value, dict):
-            yaml_str += f"{spaces}{key}:\\n{format_yaml_like(value, indent + 1)}"
-        else:
-            yaml_str += f"{spaces}{key}: {value}\\n"
-    return yaml_str
-
-
-if __name__ == "__main__":
-    if "${task.ext.prefix}" != "null":
-        prefix = "${task.ext.prefix}."
-    else:
-        prefix = "${task.ext.gtf}."
-
-    if not get_rrna_intervals("$gtf", f"{prefix}_rrna_intervals.gtf"):
-        logging.error("Failed to extract rrna transcipts.")
-
-    # Write the versions
-    versions_this_module = {}
-    versions_this_module["${task.process}"] = {"python": platform.python_version()}
-    with open("versions.yml", "w") as f:
-        f.write(format_yaml_like(versions_this_module))
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test b/modules/nf-core/rrnatranscripts/tests/main.nf.test
deleted file mode 100644
index db0d55652..000000000
--- a/modules/nf-core/rrnatranscripts/tests/main.nf.test
+++ /dev/null
@@ -1,53 +0,0 @@
-nextflow_process {
-
-    name "Test Process RRNATRANSCRIPTS"
-    script "../main.nf"
-    process "RRNATRANSCRIPTS"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "rrnatranscripts"
-
-    test("homo_sapiens - gtf") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
-                ]                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - gtf - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
-                ]                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap b/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
deleted file mode 100644
index a190ddcbf..000000000
--- a/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
+++ /dev/null
@@ -1,40 +0,0 @@
-{
-    "homo_sapiens - gtf": {
-        "content": [
-            {
-                "0": [
-                    
-                ],
-                "1": [
-                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
-                ],
-                "rrna_gtf": [
-                    
-                ],
-                "versions": [
-                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
-                ]
-            }
-        ],
-        "timestamp": "2024-05-21T13:55:44.831609"
-    },
-    "homo_sapiens - gtf - stub": {
-        "content": [
-            {
-                "0": [
-                    "genome_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "1": [
-                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
-                ],
-                "rrna_gtf": [
-                    "genome_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                ],
-                "versions": [
-                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
-                ]
-            }
-        ],
-        "timestamp": "2024-05-21T13:55:56.134136"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/rrnatranscripts/tests/tags.yml b/modules/nf-core/rrnatranscripts/tests/tags.yml
deleted file mode 100644
index ade15226a..000000000
--- a/modules/nf-core/rrnatranscripts/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-rrnatranscripts:
-  - "modules/nf-core/rrnatranscripts/**"
diff --git a/modules/nf-core/salmon/index/environment.yml b/modules/nf-core/salmon/index/environment.yml
index b3f75777e..c9d531434 100644
--- a/modules/nf-core/salmon/index/environment.yml
+++ b/modules/nf-core/salmon/index/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/salmon/index/main.nf b/modules/nf-core/salmon/index/main.nf
index 3d653c0d0..2e9c6224c 100644
--- a/modules/nf-core/salmon/index/main.nf
+++ b/modules/nf-core/salmon/index/main.nf
@@ -20,22 +20,29 @@ process SALMON_INDEX {
 
     script:
     def args = task.ext.args ?: ''
-    def get_decoy_ids = "grep '^>' $genome_fasta | cut -d ' ' -f 1 | cut -d \$'\\t' -f 1 > decoys.txt"
-    def gentrome      = "gentrome.fa"
-    if (genome_fasta.endsWith('.gz')) {
-        get_decoy_ids = "grep '^>' <(gunzip -c $genome_fasta) | cut -d ' ' -f 1 | cut -d \$'\\t' -f 1 > decoys.txt"
-        gentrome      = "gentrome.fa.gz"
+    def decoys = ''
+    def fasta = transcript_fasta
+    if (genome_fasta){
+        if (genome_fasta.endsWith('.gz')) {
+            genome_fasta = "<(gunzip -c $genome_fasta)"
+        }
+        decoys='-d decoys.txt'
+        fasta='gentrome.fa'
+    }
+    if (transcript_fasta.endsWith('.gz')) {
+        transcript_fasta = "<(gunzip -c $transcript_fasta)"
     }
     """
-    $get_decoy_ids
-    sed -i.bak -e 's/>//g' decoys.txt
-    cat $transcript_fasta $genome_fasta > $gentrome
+    if [ -n '$genome_fasta' ]; then
+        grep '^>' $genome_fasta | cut -d ' ' -f 1 | cut -d \$'\\t' -f 1 | sed 's/>//g' > decoys.txt
+        cat $transcript_fasta $genome_fasta > $fasta
+    fi
 
     salmon \\
         index \\
         --threads $task.cpus \\
-        -t $gentrome \\
-        -d decoys.txt \\
+        -t $fasta \\
+        $decoys \\
         $args \\
         -i salmon
 
diff --git a/modules/nf-core/salmon/index/tests/main.nf.test b/modules/nf-core/salmon/index/tests/main.nf.test
index 16b3c1a79..30b7359b9 100644
--- a/modules/nf-core/salmon/index/tests/main.nf.test
+++ b/modules/nf-core/salmon/index/tests/main.nf.test
@@ -26,13 +26,43 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert path(process.out.index.get(0)).exists() },
-                { assert snapshot(process.out.versions).match("versions") }
+                { assert snapshot(
+                    file(process.out.index[0]).listFiles().collect { it.getName() }.sort().toString(),
+                    process.out.versions
+                ).match()}
             )
         }
 
     }
 
-     test("sarscov2 stub") {
+    test("sarscov2 transcriptome only") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+            process {
+                """
+                input[0] = Channel.of([])
+                input[1] = Channel.of([file(params.modules_testdata_base_path + "genomics/sarscov2/genome/transcriptome.fasta", checkIfExists: true)])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert path(process.out.index.get(0)).exists() },
+                { assert snapshot(
+                    file(process.out.index[0]).listFiles().collect { it.getName() }.sort().toString(),
+                    process.out.versions
+                ).match()}
+            )
+        }
+
+    }
+
+    test("sarscov2 stub") {
         options "-stub"
         when {
             params {
@@ -50,7 +80,10 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert path(process.out.index.get(0)).exists() },
-                { assert snapshot(process.out.versions).match("versions stub") }
+                { assert snapshot(
+                    file(process.out.index[0]).listFiles().collect { it.getName() }.sort().toString(),
+                    process.out.versions
+                ).match()}
             )
         }
 
diff --git a/modules/nf-core/salmon/index/tests/main.nf.test.snap b/modules/nf-core/salmon/index/tests/main.nf.test.snap
index e5899b511..f8ed44d7d 100644
--- a/modules/nf-core/salmon/index/tests/main.nf.test.snap
+++ b/modules/nf-core/salmon/index/tests/main.nf.test.snap
@@ -1,26 +1,41 @@
 {
-    "versions": {
+    "sarscov2 stub": {
         "content": [
+            "[complete_ref_lens.bin, ctable.bin, ctg_offsets.bin, duplicate_clusters.tsv, info.json, mphf.bin, pos.bin, pre_indexing.log, rank.bin, refAccumLengths.bin, ref_indexing.log, reflengths.bin, refseq.bin, seq.bin, versionInfo.json]",
             [
                 "versions.yml:md5,85337fa0a286ea35073ee5260974e307"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-18T10:00:47.087293189"
+        "timestamp": "2025-01-20T12:57:51.498323"
     },
-    "versions stub": {
+    "sarscov2": {
         "content": [
+            "[complete_ref_lens.bin, ctable.bin, ctg_offsets.bin, duplicate_clusters.tsv, info.json, mphf.bin, pos.bin, pre_indexing.log, rank.bin, refAccumLengths.bin, ref_indexing.log, reflengths.bin, refseq.bin, seq.bin, versionInfo.json]",
             [
                 "versions.yml:md5,85337fa0a286ea35073ee5260974e307"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-18T10:01:03.89824494"
+        "timestamp": "2025-01-20T12:57:33.474302"
+    },
+    "sarscov2 transcriptome only": {
+        "content": [
+            "[complete_ref_lens.bin, ctable.bin, ctg_offsets.bin, duplicate_clusters.tsv, info.json, mphf.bin, pos.bin, pre_indexing.log, rank.bin, refAccumLengths.bin, ref_indexing.log, reflengths.bin, refseq.bin, seq.bin, versionInfo.json]",
+            [
+                "versions.yml:md5,85337fa0a286ea35073ee5260974e307"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2025-01-20T12:57:42.420247"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/salmon/index/tests/tags.yml b/modules/nf-core/salmon/index/tests/tags.yml
deleted file mode 100644
index 02997890f..000000000
--- a/modules/nf-core/salmon/index/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-salmon/index:
-  - modules/nf-core/salmon/index/**
diff --git a/modules/nf-core/salmon/quant/main.nf b/modules/nf-core/salmon/quant/main.nf
index e2d273525..b7c71871c 100644
--- a/modules/nf-core/salmon/quant/main.nf
+++ b/modules/nf-core/salmon/quant/main.nf
@@ -1,79 +1,94 @@
 process SALMON_QUANT {
-    tag "$meta.id"
+    tag "${meta.id}"
     label "process_medium"
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/salmon:1.10.3--h6dccd9a_2' :
-        'biocontainers/salmon:1.10.3--h6dccd9a_2' }"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/salmon:1.10.3--h6dccd9a_2'
+        : 'biocontainers/salmon:1.10.3--h6dccd9a_2'}"
 
     input:
     tuple val(meta), path(reads)
-    path  index
-    path  gtf
-    path  transcript_fasta
-    val   alignment_mode
-    val   lib_type
+    path index
+    path gtf
+    path transcript_fasta
+    val alignment_mode
+    val lib_type
 
     output:
-    tuple val(meta), path("${prefix}")              , emit: results
-    tuple val(meta), path("*info.json")             , emit: json_info, optional: true
+    tuple val(meta), path("${prefix}"), emit: results
+    tuple val(meta), path("*info.json"), emit: json_info, optional: true
     tuple val(meta), path("*lib_format_counts.json"), emit: lib_format_counts, optional: true
-    path  "versions.yml"                            , emit: versions
+    path "versions.yml", emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args   ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
 
-    def reference   = "--index $index"
+    def reference = "--index ${index}"
     def reads1 = []
     def reads2 = []
-    meta.single_end ? [reads].flatten().each{reads1 << it} : reads.eachWithIndex{ v, ix -> ( ix & 1 ? reads2 : reads1) << v }
+    meta.single_end ? [reads].flatten().each { reads1 << it } : reads.eachWithIndex { v, ix -> (ix & 1 ? reads2 : reads1) << v }
     def input_reads = meta.single_end ? "-r ${reads1.join(" ")}" : "-1 ${reads1.join(" ")} -2 ${reads2.join(" ")}"
     if (alignment_mode) {
-        reference   = "-t $transcript_fasta"
-        input_reads = "-a $reads"
+        reference = "-t ${transcript_fasta}"
+        input_reads = "-a ${reads}"
     }
 
     def strandedness_opts = [
-        'A', 'U', 'SF', 'SR',
-        'IS', 'IU' , 'ISF', 'ISR',
-        'OS', 'OU' , 'OSF', 'OSR',
-        'MS', 'MU' , 'MSF', 'MSR'
+        'A',
+        'U',
+        'SF',
+        'SR',
+        'IS',
+        'IU',
+        'ISF',
+        'ISR',
+        'OS',
+        'OU',
+        'OSF',
+        'OSR',
+        'MS',
+        'MU',
+        'MSF',
+        'MSR',
     ]
-    def strandedness =  'A'
+    def strandedness = 'A'
     if (lib_type) {
         if (strandedness_opts.contains(lib_type)) {
             strandedness = lib_type
-        } else {
-            log.info "[Salmon Quant] Invalid library type specified '--libType=${lib_type}', defaulting to auto-detection with '--libType=A'."
         }
-    } else {
+        else {
+            log.info("[Salmon Quant] Invalid library type specified '--libType=${lib_type}', defaulting to auto-detection with '--libType=A'.")
+        }
+    }
+    else {
         strandedness = meta.single_end ? 'U' : 'IU'
         if (meta.strandedness == 'forward') {
             strandedness = meta.single_end ? 'SF' : 'ISF'
-        } else if (meta.strandedness == 'reverse') {
+        }
+        else if (meta.strandedness == 'reverse') {
             strandedness = meta.single_end ? 'SR' : 'ISR'
         }
     }
     """
     salmon quant \\
-        --geneMap $gtf \\
-        --threads $task.cpus \\
-        --libType=$strandedness \\
-        $reference \\
-        $input_reads \\
-        $args \\
-        -o $prefix
+        --geneMap ${gtf} \\
+        --threads ${task.cpus} \\
+        --libType=${strandedness} \\
+        ${reference} \\
+        ${input_reads} \\
+        ${args} \\
+        -o ${prefix}
 
-    if [ -f $prefix/aux_info/meta_info.json ]; then
-        cp $prefix/aux_info/meta_info.json "${prefix}_meta_info.json"
+    if [ -f ${prefix}/aux_info/meta_info.json ]; then
+        cp ${prefix}/aux_info/meta_info.json "${prefix}_meta_info.json"
     fi
-    if [ -f $prefix/lib_format_counts.json ]; then
-        cp $prefix/lib_format_counts.json "${prefix}_lib_format_counts.json"
+    if [ -f ${prefix}/lib_format_counts.json ]; then
+        cp ${prefix}/lib_format_counts.json "${prefix}_lib_format_counts.json"
     fi
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/modules/nf-core/salmon/quant/tests/tags.yml b/modules/nf-core/salmon/quant/tests/tags.yml
deleted file mode 100644
index 048d81642..000000000
--- a/modules/nf-core/salmon/quant/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-salmon/quant:
-  - modules/nf-core/salmon/quant/**
diff --git a/modules/nf-core/samtools/convert/tests/tags.yml b/modules/nf-core/samtools/convert/tests/tags.yml
deleted file mode 100644
index 030d5eb5e..000000000
--- a/modules/nf-core/samtools/convert/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-samtools/convert:
-  - "modules/nf-core/samtools/convert/**"
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
index 28c0a81cf..6de0095d8 100644
--- a/modules/nf-core/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -10,9 +10,11 @@ process SAMTOOLS_FAIDX {
     input:
     tuple val(meta), path(fasta)
     tuple val(meta2), path(fai)
+    val get_sizes
 
     output:
-    tuple val(meta), path ("*.{fa,fasta}") , emit: fa , optional: true
+    tuple val(meta), path ("*.{fa,fasta}") , emit: fa, optional: true
+    tuple val(meta), path ("*.sizes")      , emit: sizes, optional: true
     tuple val(meta), path ("*.fai")        , emit: fai, optional: true
     tuple val(meta), path ("*.gzi")        , emit: gzi, optional: true
     path "versions.yml"                    , emit: versions
@@ -22,12 +24,15 @@ process SAMTOOLS_FAIDX {
 
     script:
     def args = task.ext.args ?: ''
+    def get_sizes_command = get_sizes ? "cut -f 1,2 ${fasta}.fai > ${fasta}.sizes" : ''
     """
     samtools \\
         faidx \\
         $fasta \\
         $args
 
+    ${get_sizes_command}
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
@@ -37,9 +42,15 @@ process SAMTOOLS_FAIDX {
     stub:
     def match = (task.ext.args =~ /-o(?:utput)?\s(.*)\s?/).findAll()
     def fastacmd = match[0] ? "touch ${match[0][1]}" : ''
+    def get_sizes_command = get_sizes ? "touch ${fasta}.sizes" : ''
     """
     ${fastacmd}
     touch ${fasta}.fai
+    if [[ "${fasta.extension}" == "gz" ]]; then
+        touch ${fasta}.gzi
+    fi
+
+    ${get_sizes_command}
 
     cat <<-END_VERSIONS > versions.yml
 
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
index 6721b2cb8..256a330a1 100644
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -1,9 +1,10 @@
 name: samtools_faidx
-description: Index FASTA file
+description: Index FASTA file, and optionally generate a file of chromosome sizes
 keywords:
   - index
   - fasta
   - faidx
+  - chromosome
 tools:
   - samtools:
       description: |
@@ -34,6 +35,10 @@ input:
         type: file
         description: FASTA index file
         pattern: "*.{fai}"
+  - - get_sizes:
+        type: boolean
+        description: use cut to get the sizes of the index (true) or not (false)
+
 output:
   - fa:
       - meta:
@@ -55,6 +60,16 @@ output:
           type: file
           description: FASTA index file
           pattern: "*.{fai}"
+  - sizes:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.sizes":
+          type: file
+          description: File containing chromosome lengths
+          pattern: "*.{sizes}"
   - gzi:
       - meta:
           type: map
@@ -75,6 +90,5 @@ authors:
   - "@ewels"
   - "@phue"
 maintainers:
-  - "@drpatelh"
-  - "@ewels"
+  - "@maxulysse"
   - "@phue"
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test b/modules/nf-core/samtools/faidx/tests/main.nf.test
index 17244ef2e..64219b7d9 100644
--- a/modules/nf-core/samtools/faidx/tests/main.nf.test
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test
@@ -16,8 +16,8 @@ nextflow_process {
                 """
                 input[0] = [ [ id:'test', single_end:false ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
-
                 input[1] = [[],[]]
+                input[2] = false
                 """
             }
         }
@@ -37,8 +37,8 @@ nextflow_process {
                 """
                 input[0] = [ [ id:'test', single_end:false ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)]
-
                 input[1] = [[],[]]
+                input[2] = false
                 """
             }
         }
@@ -60,9 +60,9 @@ nextflow_process {
                 """
                 input[0] = [ [ id:'test', single_end:false ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
-
                 input[1] = [ [ id:'test', single_end:false ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                input[2] = false
                 """
             }
         }
@@ -84,9 +84,9 @@ nextflow_process {
                 """
                 input[0] = [ [ id:'test', single_end:false ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
-
                 input[1] = [ [ id:'test', single_end:false ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ]
+                input[2] = false
                 """
             }
         }
@@ -106,8 +106,8 @@ nextflow_process {
                 """
                 input[0] = [ [ id:'test', single_end:false ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ]
-
                 input[1] = [[],[]]
+                input[2] = false
                 """
             }
         }
@@ -119,4 +119,101 @@ nextflow_process {
             )
         }
     }
+
+    test("test_samtools_faidx_get_sizes") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_get_sizes_bgzip") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_get_sizes - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("test_samtools_faidx_get_sizes_bgzip - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                input[2] = true
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
index 1bbb3ec2b..737224148 100644
--- a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap
@@ -6,6 +6,9 @@
                     
                 ],
                 "1": [
+                    
+                ],
+                "2": [
                     [
                         {
                             "id": "test",
@@ -14,10 +17,10 @@
                         "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
                     ]
                 ],
-                "2": [
+                "3": [
                     
                 ],
-                "3": [
+                "4": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
@@ -34,6 +37,9 @@
                 ],
                 "gzi": [
                     
+                ],
+                "sizes": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
@@ -41,10 +47,142 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:31:48.258623157"
+    },
+    "test_samtools_faidx_get_sizes_bgzip - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
         },
-        "timestamp": "2024-09-16T07:57:47.450887871"
+        "timestamp": "2024-11-20T17:32:41.122428188"
+    },
+    "test_samtools_faidx_get_sizes": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:34:02.353546697"
     },
     "test_samtools_faidx_bgzip": {
         "content": [
@@ -53,6 +191,9 @@
                     
                 ],
                 "1": [
+                    
+                ],
+                "2": [
                     [
                         {
                             "id": "test",
@@ -61,7 +202,7 @@
                         "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
                     ]
                 ],
-                "2": [
+                "3": [
                     [
                         {
                             "id": "test",
@@ -70,7 +211,7 @@
                         "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
                     ]
                 ],
-                "3": [
+                "4": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
@@ -93,6 +234,9 @@
                         },
                         "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
                     ]
+                ],
+                "sizes": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
@@ -100,10 +244,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
         },
-        "timestamp": "2024-09-16T07:58:04.804905659"
+        "timestamp": "2024-11-20T17:31:55.157487176"
     },
     "test_samtools_faidx_fasta": {
         "content": [
@@ -124,6 +268,9 @@
                     
                 ],
                 "3": [
+                    
+                ],
+                "4": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
@@ -140,6 +287,9 @@
                 ],
                 "gzi": [
                     
+                ],
+                "sizes": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
@@ -147,10 +297,71 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
         },
-        "timestamp": "2024-09-16T07:58:23.831268154"
+        "timestamp": "2024-11-20T17:32:02.149455586"
+    },
+    "test_samtools_faidx_get_sizes - stub": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.fai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "gzi": [
+                    
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.sizes:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,e3e4ba35a02020d173be8d1ee04eaebf"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:34.29376776"
     },
     "test_samtools_faidx_stub_fasta": {
         "content": [
@@ -171,6 +382,9 @@
                     
                 ],
                 "3": [
+                    
+                ],
+                "4": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
@@ -187,6 +401,9 @@
                 ],
                 "gzi": [
                     
+                ],
+                "sizes": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
@@ -194,10 +411,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
         },
-        "timestamp": "2024-09-16T07:58:35.600243706"
+        "timestamp": "2024-11-20T17:32:09.125065185"
     },
     "test_samtools_faidx_stub_fai": {
         "content": [
@@ -206,6 +423,9 @@
                     
                 ],
                 "1": [
+                    
+                ],
+                "2": [
                     [
                         {
                             "id": "test",
@@ -214,10 +434,10 @@
                         "genome.fasta.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
                     ]
                 ],
-                "2": [
+                "3": [
                     
                 ],
-                "3": [
+                "4": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
                 ],
                 "fa": [
@@ -234,6 +454,80 @@
                 ],
                 "gzi": [
                     
+                ],
+                "sizes": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
+        },
+        "timestamp": "2024-11-20T17:32:16.274287863"
+    },
+    "test_samtools_faidx_get_sizes_bgzip": {
+        "content": [
+            {
+                "0": [
+                    
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "4": [
+                    "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
+                ],
+                "fa": [
+                    
+                ],
+                "fai": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.fai:md5,9da2a56e2853dc8c0b86a9e7229c9fe5"
+                    ]
+                ],
+                "gzi": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.gzi:md5,7dea362b3fac8e00956a4952a3d4f474"
+                    ]
+                ],
+                "sizes": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "genome.fasta.gz.sizes:md5,a57c401f27ae5133823fb09fb21c8a3c"
+                    ]
                 ],
                 "versions": [
                     "versions.yml:md5,6bbe80a2e14bd61202ca63e12d66027f"
@@ -241,9 +535,9 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.1"
         },
-        "timestamp": "2024-09-16T07:58:54.705460167"
+        "timestamp": "2024-11-20T17:32:28.117654855"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/faidx/tests/tags.yml b/modules/nf-core/samtools/faidx/tests/tags.yml
deleted file mode 100644
index e4a839481..000000000
--- a/modules/nf-core/samtools/faidx/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-samtools/faidx:
-  - modules/nf-core/samtools/faidx/**
diff --git a/modules/nf-core/samtools/index/tests/tags.yml b/modules/nf-core/samtools/index/tests/tags.yml
deleted file mode 100644
index e0f58a7a3..000000000
--- a/modules/nf-core/samtools/index/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-samtools/index:
-  - modules/nf-core/samtools/index/**
diff --git a/modules/nf-core/samtools/sort/tests/tags.yml b/modules/nf-core/samtools/sort/tests/tags.yml
deleted file mode 100644
index cd63ea208..000000000
--- a/modules/nf-core/samtools/sort/tests/tags.yml
+++ /dev/null
@@ -1,3 +0,0 @@
-samtools/sort:
-  - modules/nf-core/samtools/sort/**
-  - tests/modules/nf-core/samtools/sort/**
diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml
deleted file mode 100644
index 02cda6e6a..000000000
--- a/modules/nf-core/samtools/view/environment.yml
+++ /dev/null
@@ -1,10 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.21
-  # renovate: datasource=conda depName=bioconda/samtools
-  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
deleted file mode 100644
index a6941e638..000000000
--- a/modules/nf-core/samtools/view/main.nf
+++ /dev/null
@@ -1,77 +0,0 @@
-process SAMTOOLS_VIEW {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9e/9edc2564215d5cd137a8b25ca8a311600987186d406b092022444adf3c4447f7/data' :
-        'community.wave.seqera.io/library/htslib_samtools:1.21--6cb89bfd40cbaabf' }"
-
-    input:
-    tuple val(meta), path(input), path(index)
-    tuple val(meta2), path(fasta)
-    path qname
-
-    output:
-    tuple val(meta), path("${prefix}.bam"),                                    emit: bam,              optional: true
-    tuple val(meta), path("${prefix}.cram"),                                   emit: cram,             optional: true
-    tuple val(meta), path("${prefix}.sam"),                                    emit: sam,              optional: true
-    tuple val(meta), path("${prefix}.${file_type}.bai"),                       emit: bai,              optional: true
-    tuple val(meta), path("${prefix}.${file_type}.csi"),                       emit: csi,              optional: true
-    tuple val(meta), path("${prefix}.${file_type}.crai"),                      emit: crai,             optional: true
-    tuple val(meta), path("${prefix}.unselected.${file_type}"),                emit: unselected,       optional: true
-    tuple val(meta), path("${prefix}.unselected.${file_type}.{bai,csi,crsi}"), emit: unselected_index, optional: true
-    path  "versions.yml",                                                      emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    def reference = fasta ? "--reference ${fasta}" : ""
-    file_type = args.contains("--output-fmt sam") ? "sam" :
-                args.contains("--output-fmt bam") ? "bam" :
-                args.contains("--output-fmt cram") ? "cram" :
-                input.getExtension()
-    readnames = qname ? "--qname-file ${qname} --output-unselected ${prefix}.unselected.${file_type}": ""
-    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
-    """
-    samtools \\
-        view \\
-        --threads ${task.cpus-1} \\
-        ${reference} \\
-        ${readnames} \\
-        $args \\
-        -o ${prefix}.${file_type} \\
-        $input \\
-        $args2
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    def args = task.ext.args ?: ''
-    prefix = task.ext.prefix ?: "${meta.id}"
-    file_type = args.contains("--output-fmt sam") ? "sam" :
-                args.contains("--output-fmt bam") ? "bam" :
-                args.contains("--output-fmt cram") ? "cram" :
-                input.getExtension()
-    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
-
-    index = args.contains("--write-index") ? "touch ${prefix}.${file_type}.csi" : ""
-
-    """
-    touch ${prefix}.${file_type}
-    ${index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
deleted file mode 100644
index caa7b0150..000000000
--- a/modules/nf-core/samtools/view/meta.yml
+++ /dev/null
@@ -1,141 +0,0 @@
-name: samtools_view
-description: filter/convert SAM/BAM/CRAM file
-keywords:
-  - view
-  - bam
-  - sam
-  - cram
-tools:
-  - samtools:
-      description: |
-        SAMtools is a set of utilities for interacting with and post-processing
-        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
-        These files are generated as output by short read aligners like BWA.
-      homepage: http://www.htslib.org/
-      documentation: http://www.htslib.org/doc/samtools.html
-      doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
-      identifier: biotools:samtools
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - input:
-        type: file
-        description: BAM/CRAM/SAM file
-        pattern: "*.{bam,cram,sam}"
-    - index:
-        type: file
-        description: BAM.BAI/BAM.CSI/CRAM.CRAI file (optional)
-        pattern: "*.{.bai,.csi,.crai}"
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
-    - fasta:
-        type: file
-        description: Reference file the CRAM was created with (optional)
-        pattern: "*.{fasta,fa}"
-  - - qname:
-        type: file
-        description: Optional file with read names to output only select alignments
-        pattern: "*.{txt,list}"
-output:
-  - bam:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.bam:
-          type: file
-          description: optional filtered/converted BAM file
-          pattern: "*.{bam}"
-  - cram:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.cram:
-          type: file
-          description: optional filtered/converted CRAM file
-          pattern: "*.{cram}"
-  - sam:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.sam:
-          type: file
-          description: optional filtered/converted SAM file
-          pattern: "*.{sam}"
-  - bai:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.${file_type}.bai:
-          type: file
-          description: optional BAM file index
-          pattern: "*.{bai}"
-  - csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.${file_type}.csi:
-          type: file
-          description: optional tabix BAM file index
-          pattern: "*.{csi}"
-  - crai:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.${file_type}.crai:
-          type: file
-          description: optional CRAM file index
-          pattern: "*.{crai}"
-  - unselected:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.unselected.${file_type}:
-          type: file
-          description: optional file with unselected alignments
-          pattern: "*.unselected.{bam,cram,sam}"
-  - unselected_index:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.unselected.${file_type}.{bai,csi,crsi}:
-          type: file
-          description: index for the "unselected" file
-          pattern: "*.unselected.{bai,csi,crai}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@drpatelh"
-  - "@joseespinosa"
-  - "@FriederikeHanssen"
-  - "@priyanka-surana"
-maintainers:
-  - "@drpatelh"
-  - "@joseespinosa"
-  - "@FriederikeHanssen"
-  - "@priyanka-surana"
diff --git a/modules/nf-core/samtools/view/tests/bam.config b/modules/nf-core/samtools/view/tests/bam.config
deleted file mode 100644
index c10d10811..000000000
--- a/modules/nf-core/samtools/view/tests/bam.config
+++ /dev/null
@@ -1,3 +0,0 @@
-process {
-    ext.args = "--output-fmt bam"
-}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/bam_index.config b/modules/nf-core/samtools/view/tests/bam_index.config
deleted file mode 100644
index 771ae033a..000000000
--- a/modules/nf-core/samtools/view/tests/bam_index.config
+++ /dev/null
@@ -1,3 +0,0 @@
-process {
-    ext.args = "--output-fmt bam --write-index"
-}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test b/modules/nf-core/samtools/view/tests/main.nf.test
deleted file mode 100644
index 37b81a916..000000000
--- a/modules/nf-core/samtools/view/tests/main.nf.test
+++ /dev/null
@@ -1,214 +0,0 @@
-nextflow_process {
-
-    name "Test Process SAMTOOLS_VIEW"
-    script "../main.nf"
-    process "SAMTOOLS_VIEW"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "samtools"
-    tag "samtools/view"
-
-    test("bam") {
-
-        when {
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true),
-                    []
-                ])
-                input[1] = [[],[]]
-                input[2] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_bam") },
-                { assert snapshot(process.out.bai).match("bam_bai") },
-                { assert snapshot(process.out.crai).match("bam_crai") },
-                { assert snapshot(process.out.cram).match("bam_cram") },
-                { assert snapshot(process.out.csi).match("bam_csi") },
-                { assert snapshot(process.out.sam).match("bam_sam") },
-                { assert snapshot(process.out.versions).match("bam_versions") }
-            )
-        }
-    }
-
-    test("cram") {
-
-        when {
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
-                ])
-                input[1] = Channel.of([
-                    [ id:'genome' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ])
-                input[2] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(file(process.out.cram[0][1]).name).match("cram_cram") },
-                { assert snapshot(process.out.bai).match("cram_bai") },
-                { assert snapshot(process.out.bam).match("cram_bam") },
-                { assert snapshot(process.out.crai).match("cram_crai") },
-                { assert snapshot(process.out.csi).match("cram_csi") },
-                { assert snapshot(process.out.sam).match("cram_sam") },
-                { assert snapshot(process.out.versions).match("cram_versions") }
-            )
-        }
-    }
-
-    test("cram_to_bam") {
-
-        config "./bam.config"
-
-        when {
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
-                    []
-                ])
-                input[1] = Channel.of([
-                    [ id:'genome' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ])
-                input[2] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_bam") },
-                { assert snapshot(process.out.bai).match("cram_to_bam_bai") },
-                { assert snapshot(process.out.crai).match("cram_to_bam_crai") },
-                { assert snapshot(process.out.cram).match("cram_to_bam_cram") },
-                { assert snapshot(process.out.csi).match("cram_to_bam_csi") },
-                { assert snapshot(process.out.sam).match("cram_to_bam_sam") },
-                { assert snapshot(process.out.versions).match("cram_to_bam_versions") }
-            )
-        }
-    }
-
-    test("cram_to_bam_index") {
-
-        config "./bam_index.config"
-
-        when {
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
-                    []
-                ])
-                input[1] = Channel.of([
-                    [ id:'genome' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ])
-                input[2] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_bam") },
-                { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_csi") },
-                { assert snapshot(process.out.bai).match("cram_to_bam_index_bai") },
-                { assert snapshot(process.out.crai).match("cram_to_bam_index_crai") },
-                { assert snapshot(process.out.cram).match("cram_to_bam_index_cram") },
-                { assert snapshot(process.out.sam).match("cram_to_bam_index_sam") },
-                { assert snapshot(process.out.versions).match("cram_to_bam_index_versions") }
-            )
-        }
-    }
-
-    test("cram_to_bam_index_qname") {
-
-        config "./bam_index.config"
-
-        when {
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
-                    []
-                ])
-                input[1] = Channel.of([
-                    [ id:'genome' ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
-                ])
-                input[2] = Channel.of("testN:2817", "testN:2814").collectFile(name: "readnames.list", newLine: true)
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_index_qname_bam") },
-                { assert snapshot(file(process.out.csi[0][1]).name).match("cram_to_bam_index_qname_csi") },
-                { assert snapshot(process.out.bai).match("cram_to_bam_index_qname_bai") },
-                { assert snapshot(process.out.crai).match("cram_to_bam_index_qname_crai") },
-                { assert snapshot(process.out.cram).match("cram_to_bam_index_qname_cram") },
-                { assert snapshot(process.out.sam).match("cram_to_bam_index_qname_sam") },
-                { assert snapshot(file(process.out.unselected[0][1]).name).match("cram_to_bam_index_qname_unselected") },
-                { assert snapshot(file(process.out.unselected_index[0][1]).name).match("cram_to_bam_index_qname_unselected_csi") },
-                { assert snapshot(process.out.versions).match("cram_to_bam_index_qname_versions") }
-            )
-        }
-    }
-
-    test("bam_stub") {
-
-        options "-stub"
-        config "./bam_index.config"
-
-        when {
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true),
-                    []
-                ])
-                input[1] = [[],[]]
-                input[2] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(file(process.out.bam[0][1]).name).match("bam_stub_bam") },
-                { assert snapshot(file(process.out.csi[0][1]).name).match("bam_stub_csi") },
-                { assert snapshot(process.out.bai).match("bam_stub_bai") },
-                { assert snapshot(process.out.crai).match("bam_stub_crai") },
-                { assert snapshot(process.out.cram).match("bam_stub_cram") },
-                { assert snapshot(process.out.sam).match("bam_stub_sam") },
-                { assert snapshot(process.out.versions).match("bam_stub_versions") }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/samtools/view/tests/main.nf.test.snap b/modules/nf-core/samtools/view/tests/main.nf.test.snap
deleted file mode 100644
index 63849b037..000000000
--- a/modules/nf-core/samtools/view/tests/main.nf.test.snap
+++ /dev/null
@@ -1,528 +0,0 @@
-{
-    "bam_bam": {
-        "content": [
-            "test.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:51.256068"
-    },
-    "cram_to_bam_index_csi": {
-        "content": [
-            "test.bam.csi"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:12.958617"
-    },
-    "bam_stub_bam": {
-        "content": [
-            "test.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:32.065301"
-    },
-    "bam_bai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:51.258578"
-    },
-    "bam_stub_bai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:32.071284"
-    },
-    "bam_stub_versions": {
-        "content": [
-            [
-                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-16T09:26:24.461775464"
-    },
-    "cram_to_bam_index_cram": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:12.972288"
-    },
-    "cram_to_bam_sam": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:04.999247"
-    },
-    "cram_to_bam_index_sam": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:12.976457"
-    },
-    "cram_crai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:56.497581"
-    },
-    "cram_csi": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:56.50038"
-    },
-    "cram_to_bam_cram": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:04.992239"
-    },
-    "cram_to_bam_index_qname_csi": {
-        "content": [
-            "test.bam.csi"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.325496"
-    },
-    "bam_stub_sam": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:32.079529"
-    },
-    "cram_cram": {
-        "content": [
-            "test.cram"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:56.490286"
-    },
-    "cram_to_bam_index_qname_unselected_csi": {
-        "content": [
-            "test.unselected.bam.csi"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.328458"
-    },
-    "bam_csi": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:51.262882"
-    },
-    "cram_to_bam_crai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:04.989247"
-    },
-    "cram_to_bam_index_crai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:12.967681"
-    },
-    "cram_to_bam_index_qname_versions": {
-        "content": [
-            [
-                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-16T09:25:51.953436682"
-    },
-    "cram_to_bam_bam": {
-        "content": [
-            "test.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:04.982361"
-    },
-    "cram_to_bam_index_bam": {
-        "content": [
-            "test.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:12.95456"
-    },
-    "cram_to_bam_index_versions": {
-        "content": [
-            [
-                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-16T09:25:14.475388399"
-    },
-    "cram_to_bam_bai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:04.98601"
-    },
-    "cram_to_bam_versions": {
-        "content": [
-            [
-                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-16T09:24:49.673441798"
-    },
-    "cram_bam": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:56.495512"
-    },
-    "bam_stub_cram": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:32.076908"
-    },
-    "cram_to_bam_index_qname_bai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.328458"
-    },
-    "cram_to_bam_index_qname_crai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.330789"
-    },
-    "cram_bai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:56.493129"
-    },
-    "bam_stub_crai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:32.074313"
-    },
-    "cram_to_bam_index_qname_bam": {
-        "content": [
-            "test.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.322874"
-    },
-    "cram_to_bam_index_qname_unselected": {
-        "content": [
-            "test.unselected.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.322874"
-    },
-    "cram_to_bam_index_qname_unselected_csi": {
-        "content": [
-            "test.unselected.bam.csi"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.328458"
-    },
-    "bam_versions": {
-        "content": [
-            [
-                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-16T09:23:27.151650338"
-    },
-    "cram_to_bam_index_qname_cram": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.333248"
-    },
-    "bam_crai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:51.259774"
-    },
-    "bam_cram": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:51.261287"
-    },
-    "cram_to_bam_csi": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:04.995454"
-    },
-    "cram_sam": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:56.502625"
-    },
-    "cram_versions": {
-        "content": [
-            [
-                "versions.yml:md5,176db5ec46b965219604bcdbb3ef9e07"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
-        },
-        "timestamp": "2024-09-16T09:24:12.95416913"
-    },
-    "cram_to_bam_index_qname_unselected": {
-        "content": [
-            "test.unselected.bam"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.322874"
-    },
-    "bam_sam": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:37:51.264651"
-    },
-    "cram_to_bam_index_bai": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:12.962863"
-    },
-    "cram_to_bam_index_qname_sam": {
-        "content": [
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:23.337634"
-    },
-    "bam_stub_csi": {
-        "content": [
-            "test.bam.csi"
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.04.3"
-        },
-        "timestamp": "2024-02-12T19:38:32.068596"
-    }
-}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/view/tests/tags.yml b/modules/nf-core/samtools/view/tests/tags.yml
deleted file mode 100644
index 4fdf1dd12..000000000
--- a/modules/nf-core/samtools/view/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-samtools/view:
-  - "modules/nf-core/samtools/view/**"
diff --git a/modules/nf-core/star/align/tests/tags.yml b/modules/nf-core/star/align/tests/tags.yml
deleted file mode 100644
index 8beace16e..000000000
--- a/modules/nf-core/star/align/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-star/align:
-  - modules/nf-core/star/align/**
diff --git a/modules/nf-core/star/genomegenerate/environment.yml b/modules/nf-core/star/genomegenerate/environment.yml
index 7c57530a3..91e37ae12 100644
--- a/modules/nf-core/star/genomegenerate/environment.yml
+++ b/modules/nf-core/star/genomegenerate/environment.yml
@@ -1,9 +1,11 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 
 dependencies:
-  - bioconda::htslib=1.20
-  - bioconda::samtools=1.20
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
   - bioconda::star=2.7.11b
   - conda-forge::gawk=5.1.0
diff --git a/modules/nf-core/star/genomegenerate/main.nf b/modules/nf-core/star/genomegenerate/main.nf
index 8f0c67e76..4eb95654b 100644
--- a/modules/nf-core/star/genomegenerate/main.nf
+++ b/modules/nf-core/star/genomegenerate/main.nf
@@ -4,8 +4,8 @@ process STAR_GENOMEGENERATE {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b4/b425bc2a95806d878993f9a66dae3ae80ac2dafff4c208c5ae01b7a90a32fa91/data' :
-        'community.wave.seqera.io/library/star_samtools_htslib_gawk:10c6e8c834460019' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/26/268b4c9c6cbf8fa6606c9b7fd4fafce18bf2c931d1a809a0ce51b105ec06c89d/data' :
+        'community.wave.seqera.io/library/htslib_samtools_star_gawk:ae438e9a604351a4' }"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
index 3db256782..b79da991d 100644
--- a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
+++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap
@@ -3,14 +3,14 @@
         "content": [
             "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]",
             [
-                "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
+                "versions.yml:md5,0c6c5fab50cc8601f9072268a2f49aad"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-19T20:37:47.410432728"
+        "timestamp": "2024-12-11T16:29:40.748676908"
     },
     "fasta_gtf_stub": {
         "content": [
@@ -41,7 +41,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
+                    "versions.yml:md5,0c6c5fab50cc8601f9072268a2f49aad"
                 ],
                 "index": [
                     [
@@ -69,15 +69,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
+                    "versions.yml:md5,0c6c5fab50cc8601f9072268a2f49aad"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-19T20:38:09.165234795"
+        "timestamp": "2024-12-11T16:30:11.024076742"
     },
     "fasta_stub": {
         "content": [
@@ -101,7 +101,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
+                    "versions.yml:md5,0c6c5fab50cc8601f9072268a2f49aad"
                 ],
                 "index": [
                     [
@@ -122,27 +122,27 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
+                    "versions.yml:md5,0c6c5fab50cc8601f9072268a2f49aad"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-19T20:38:19.530862664"
+        "timestamp": "2024-12-11T16:30:22.674364031"
     },
     "fasta": {
         "content": [
             "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]",
             [
-                "versions.yml:md5,14b05d04c9eca568e9ed4888aaf26fa6"
+                "versions.yml:md5,0c6c5fab50cc8601f9072268a2f49aad"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
         },
-        "timestamp": "2024-10-19T20:37:58.667436398"
+        "timestamp": "2024-12-11T16:29:57.958812354"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/star/genomegenerate/tests/tags.yml b/modules/nf-core/star/genomegenerate/tests/tags.yml
deleted file mode 100644
index 79f619bfe..000000000
--- a/modules/nf-core/star/genomegenerate/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-star/genomegenerate:
-  - modules/nf-core/star/genomegenerate/**
diff --git a/modules/nf-core/stringtie/merge/environment.yml b/modules/nf-core/stringtie/merge/environment.yml
index 0556de41a..ef1f3d0e1 100644
--- a/modules/nf-core/stringtie/merge/environment.yml
+++ b/modules/nf-core/stringtie/merge/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/stringtie/merge/tests/tags.yml b/modules/nf-core/stringtie/merge/tests/tags.yml
deleted file mode 100644
index 58cef46ba..000000000
--- a/modules/nf-core/stringtie/merge/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-stringtie/merge:
-  - modules/nf-core/stringtie/merge/**
diff --git a/modules/nf-core/stringtie/stringtie/environment.yml b/modules/nf-core/stringtie/stringtie/environment.yml
index 906b7486c..a51985b4f 100644
--- a/modules/nf-core/stringtie/stringtie/environment.yml
+++ b/modules/nf-core/stringtie/stringtie/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/stringtie/stringtie/tests/tags.yml b/modules/nf-core/stringtie/stringtie/tests/tags.yml
deleted file mode 100644
index da9b051c3..000000000
--- a/modules/nf-core/stringtie/stringtie/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-stringtie/stringtie:
-  - modules/nf-core/stringtie/stringtie/**
diff --git a/modules/nf-core/ucsc/gtftogenepred/environment.yml b/modules/nf-core/ucsc/gtftogenepred/environment.yml
index 5c4f6c2fa..86709a0d4 100644
--- a/modules/nf-core/ucsc/gtftogenepred/environment.yml
+++ b/modules/nf-core/ucsc/gtftogenepred/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index c9729ed64..1767a6123 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -56,7 +56,7 @@ workflow BUILD_REFERENCES {
 
     def ch_fai = Channel.empty()
     if (!exists_not_empty(params.fai)){
-        SAMTOOLS_FAIDX(ch_fasta, [[],[]])
+        SAMTOOLS_FAIDX(ch_fasta, [[],[]], false)
         ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions)
         ch_fai = SAMTOOLS_FAIDX.out.fai
     } else {
diff --git a/subworkflows/local/starfusion_workflow/tests/nextflow.config b/subworkflows/local/starfusion_workflow/tests/nextflow.config
index 718add69c..2f955abc4 100644
--- a/subworkflows/local/starfusion_workflow/tests/nextflow.config
+++ b/subworkflows/local/starfusion_workflow/tests/nextflow.config
@@ -30,8 +30,4 @@ process {
         ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
     }
 
-    withName: 'SAMTOOLS_VIEW_FOR_STARFUSION' {
-        ext.args = { "--output-fmt cram" }
-        ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
-    }
 }

From 525aee2fec64acab93fb7cf415a261d9aff7cd3f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 13:21:08 +0200
Subject: [PATCH 494/887] update snap

---
 tests/test_stub.nf.test.snap | 56 ++++++++++++++++++------------------
 1 file changed, 28 insertions(+), 28 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 8ab99e9b8..4d70e895b 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -38,11 +38,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -208,10 +208,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:48:48.9071057"
+        "timestamp": "2025-05-07T13:06:26.949894702"
     },
     "stub test stringtie": {
         "content": [
@@ -555,7 +555,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -863,10 +863,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:51:45.508140056"
+        "timestamp": "2025-05-07T13:08:57.754362685"
     },
     "stub test salmon": {
         "content": [
@@ -1005,7 +1005,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1184,10 +1184,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:47:44.413622274"
+        "timestamp": "2025-05-07T13:05:33.800125465"
     },
     "stub test fusionreport": {
         "content": [
@@ -1247,11 +1247,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1446,17 +1446,17 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:50:05.389865001"
+        "timestamp": "2025-05-07T13:07:32.131686727"
     },
     "stub test fastp": {
         "content": [
             10,
             {
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1520,10 +1520,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:50:34.188288253"
+        "timestamp": "2025-05-07T13:08:02.208707357"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1618,7 +1618,7 @@
                     "ctat-splicing": "0.0.2"
                 },
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1686,11 +1686,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -2071,9 +2071,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:46:29.001694439"
+        "timestamp": "2025-05-07T13:04:26.194367038"
     }
 }
\ No newline at end of file

From 4f84b13d24e3c3bcc318b5fb5bb57e615b41332f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 13:24:10 +0200
Subject: [PATCH 495/887] prettier

---
 modules.json                                  | 122 +++++-------------
 .../gatk4/markduplicates/environment.yml      |   2 +-
 2 files changed, 32 insertions(+), 92 deletions(-)

diff --git a/modules.json b/modules.json
index 8bb54eef5..844a7b83d 100644
--- a/modules.json
+++ b/modules.json
@@ -8,191 +8,137 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "1719e20e3881b37b9e1e8498ea669a2b92e54b9a",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/download": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "2a227f75f33415f74a884ef8b33555b151e33de9",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "1ec937ab3edc307bc0d79a2200d784e9f0868359",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "7b50cb7be890e4b28cffb82e438cc6a8d7805d3f",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "980a4964be91da75fb3ccf576118a18e247f6d15",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/convert": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -201,26 +147,20 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/gatk4/markduplicates/environment.yml b/modules/nf-core/gatk4/markduplicates/environment.yml
index 352d20f83..ca0fc1923 100644
--- a/modules/nf-core/gatk4/markduplicates/environment.yml
+++ b/modules/nf-core/gatk4/markduplicates/environment.yml
@@ -9,4 +9,4 @@ dependencies:
   - bioconda::gatk4=4.6.1.0
   - bioconda::gcnvkernel=0.9
   - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
\ No newline at end of file
+  - bioconda::samtools=1.21

From de370ca91cf0562144b086fdb42682930df1eaa2 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 7 May 2025 14:11:40 +0200
Subject: [PATCH 496/887] add --limit_bam_sort_ram

---
 conf/modules.config  | 45 +++++++++++++++++++++++---------------------
 nextflow.config      |  1 +
 nextflow_schema.json |  6 ++++++
 3 files changed, 31 insertions(+), 21 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index a97d0806c..662622628 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -254,27 +254,30 @@ process {
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
-        ext.args = { "--outReadsUnmapped None  \
-            --outSAMattrRGline 'ID:${meta.id}' 'SM:${meta.id}'${meta.seq_center ? " 'CN:${meta.seq_center}'": ''}${meta.seq_platform ? "' PL:${meta.seq_platform}'": ''} \
-            --outSAMstrandField intronMotif \
-            --chimOutJunctionFormat 1 \
-            --twopassMode None \
-            --outFilterMultimapNmax 50 \
-            --chimMultimapNmax 50 \
-            --quantMode GeneCounts \
-            --outSAMunmapped Within \
-            --readFilesCommand zcat  \
-            --alignSJstitchMismatchNmax 5 -1 5 5 \
-            --outSAMtype BAM SortedByCoordinate \
-            --chimSegmentMin 10 \
-            --peOverlapNbasesMin 10 \
-            --alignSplicedMateMapLminOverLmate 0.5 \
-            --chimJunctionOverhangMin 10 \
-            --chimScoreJunctionNonGTAG 0 \
-            --chimScoreDropMax 30 \
-            --chimScoreSeparation 1  \
-            --chimSegmentReadGapMax 3 \
-            --chimOutType Junctions WithinBAM" }
+        ext.args = { [
+            "--outReadsUnmapped None",
+            "--outSAMattrRGline 'ID:${meta.id}' 'SM:${meta.id}'${meta.seq_center ? " 'CN:${meta.seq_center}'": ''}${meta.seq_platform ? "' PL:${meta.seq_platform}'": ''}",
+            "--outSAMstrandField intronMotif",
+            "--chimOutJunctionFormat 1",
+            "--twopassMode None",
+            "--outFilterMultimapNmax 50",
+            "--chimMultimapNmax 50",
+            "--quantMode GeneCounts",
+            "--outSAMunmapped Within",
+            "--readFilesCommand zcat ",
+            "--alignSJstitchMismatchNmax 5 -1 5 5",
+            "--outSAMtype BAM SortedByCoordinate",
+            "--chimSegmentMin 10",
+            "--peOverlapNbasesMin 10",
+            "--alignSplicedMateMapLminOverLmate 0.5",
+            "--chimJunctionOverhangMin 10",
+            "--chimScoreJunctionNonGTAG 0",
+            "--chimScoreDropMax 30",
+            "--chimScoreSeparation 1 ",
+            "--chimSegmentReadGapMax 3",
+            "--chimOutType Junctions WithinBAM",
+            params.star_limit_bam_sort_ram ? "--limitBAMsortRAM ${params.star_limit_bam_sort_ram}" : ""
+            ].join(' ') }
     }
 
     withName: 'STAR_GENOMEGENERATE' {
diff --git a/nextflow.config b/nextflow.config
index cb6f13a62..c2e185e7c 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -46,6 +46,7 @@ params {
 
     // Alignment options
     star_ignore_sjdbgtf                  = false
+    star_limit_bam_sort_ram              = 0
     seq_center                           = ""
     seq_platform                         = ""
     fusioncatcher_limitSjdbInsertNsj     = 2000000
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 190b98292..2ef037604 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -529,6 +529,12 @@
                     "hidden": true,
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "Setting false will use GTF file for STAR alignment"
+                },
+                "star_limit_bam_sort_ram": {
+                    "type": "integer",
+                    "description": "The maximum amount of RAM to use for sorting the BAM file in STAR. Should by in bits",
+                    "fa_icon": "fas fa-toolbox",
+                    "minimum": 0
                 }
             }
         }

From dfc7efeb91db4ca73d9734338ad7bd460a6ffecc Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 7 May 2025 14:13:32 +0200
Subject: [PATCH 497/887] changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 83516b786..7f066ac39 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -42,6 +42,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
 - Moved strandedness determination for picard collectrnaseqmetrics into modules.config [#658](https://github.com/nf-core/rnafusion/pull/658)
 - Using option `--human_gencode_filter` while building STARFusion references for human species [#657](https://github.com/nf-core/rnafusion/pull/657)
+- Added `--star_limit_bam_sort_ram` to set the maximum memory for sorting the BAM files in STAR. [#668](https://github.com/nf-core/rnafusion/pull/668)
 
 ### Changed
 

From 98cadc532bff3042d045ab49d3591f545d619c65 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 7 May 2025 14:19:37 +0200
Subject: [PATCH 498/887] clarify description

---
 nextflow_schema.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 2ef037604..978c44767 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -532,7 +532,7 @@
                 },
                 "star_limit_bam_sort_ram": {
                     "type": "integer",
-                    "description": "The maximum amount of RAM to use for sorting the BAM file in STAR. Should by in bits",
+                    "description": "The maximum amount of RAM to use for sorting the BAM file in STAR. Should by in bits. Setting this value to `0` will use the default amount of STAR.",
                     "fa_icon": "fas fa-toolbox",
                     "minimum": 0
                 }

From 0c32e4777e3992d55375719ebae07ad897ca7160 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 7 May 2025 14:20:00 +0200
Subject: [PATCH 499/887] clarify the check

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index 662622628..5652213dc 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -276,7 +276,7 @@ process {
             "--chimScoreSeparation 1 ",
             "--chimSegmentReadGapMax 3",
             "--chimOutType Junctions WithinBAM",
-            params.star_limit_bam_sort_ram ? "--limitBAMsortRAM ${params.star_limit_bam_sort_ram}" : ""
+            params.star_limit_bam_sort_ram > 0 ? "--limitBAMsortRAM ${params.star_limit_bam_sort_ram}" : ""
             ].join(' ') }
     }
 

From a29e06a830fe150e65fc841f8af0c85f06098958 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 14:38:07 +0200
Subject: [PATCH 500/887] update snap

---
 tests/test_build.nf.test.snap    |  16 ++--
 tests/test_stub.nf.test.snap     | 124 +++++++++++++-------------
 tests/test_stub_bam.nf.test.snap | 148 +++++++++++++++----------------
 3 files changed, 144 insertions(+), 144 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 5c5d08924..ab452ad38 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -7,7 +7,7 @@
                     "arriba_download": "2.4.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -35,11 +35,11 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -220,7 +220,7 @@
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
-                "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+                "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -277,9 +277,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:08:10.68706064"
+        "timestamp": "2025-05-07T14:33:25.428259571"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 7495d02c4..4ce83763c 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -13,8 +13,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -38,11 +38,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -51,7 +51,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -208,10 +208,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:48:48.9071057"
+        "timestamp": "2025-05-07T14:03:38.544810125"
     },
     "stub test stringtie": {
         "content": [
@@ -227,8 +227,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -258,7 +258,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -348,10 +348,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:49:24.923946194"
+        "timestamp": "2025-05-07T14:04:11.405954818"
     },
     "stub test arriba": {
         "content": [
@@ -373,8 +373,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -404,7 +404,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -489,10 +489,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:47:08.02222829"
+        "timestamp": "2025-05-07T14:02:08.078812972"
     },
     "stub test fusioninspector": {
         "content": [
@@ -514,10 +514,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -526,8 +526,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -555,7 +555,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -564,7 +564,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -863,10 +863,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:51:45.508140056"
+        "timestamp": "2025-05-07T14:06:10.151871018"
     },
     "stub test salmon": {
         "content": [
@@ -979,8 +979,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1005,7 +1005,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1014,7 +1014,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1184,10 +1184,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:47:44.413622274"
+        "timestamp": "2025-05-07T14:02:40.610644223"
     },
     "stub test fusionreport": {
         "content": [
@@ -1210,10 +1210,10 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1222,8 +1222,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1247,11 +1247,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1260,7 +1260,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1446,17 +1446,17 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:50:05.389865001"
+        "timestamp": "2025-05-07T14:04:42.856027662"
     },
     "stub test fastp": {
         "content": [
             10,
             {
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1520,10 +1520,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:50:34.188288253"
+        "timestamp": "2025-05-07T13:08:02.208707357"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1618,7 +1618,7 @@
                     "ctat-splicing": "0.0.2"
                 },
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1637,10 +1637,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1649,8 +1649,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1686,11 +1686,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1699,7 +1699,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -2071,9 +2071,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:46:29.001694439"
+        "timestamp": "2025-05-07T14:01:35.330928392"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 99b8e5e31..69575e5cd 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -13,8 +13,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -35,15 +35,15 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -181,10 +181,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:16:16.851201431"
+        "timestamp": "2025-05-07T13:56:57.989800452"
     },
     "stub test stringtie": {
         "content": [
@@ -200,8 +200,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -223,7 +223,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -297,10 +297,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:16:59.945308111"
+        "timestamp": "2025-05-07T13:28:43.569555821"
     },
     "stub test arriba": {
         "content": [
@@ -322,8 +322,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -345,7 +345,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -411,10 +411,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:14:09.827324661"
+        "timestamp": "2025-05-07T13:26:43.570224768"
     },
     "stub test fusioninspector": {
         "content": [
@@ -436,10 +436,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -448,8 +448,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -474,11 +474,11 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -761,10 +761,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:20:00.302967276"
+        "timestamp": "2025-05-07T13:30:43.376109569"
     },
     "stub test salmon": {
         "content": [
@@ -780,8 +780,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -871,10 +871,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:19:13.383274003"
+        "timestamp": "2025-05-07T13:30:08.850942124"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -896,8 +896,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -919,11 +919,11 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1077,10 +1077,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:14:53.085896038"
+        "timestamp": "2025-05-07T13:27:17.26404911"
     },
     "stub test fusionreport": {
         "content": [
@@ -1103,10 +1103,10 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1115,8 +1115,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1137,15 +1137,15 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1312,17 +1312,17 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:17:44.757543251"
+        "timestamp": "2025-05-07T13:58:03.351087941"
     },
     "stub test fastp": {
         "content": [
             13,
             {
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1337,8 +1337,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1405,10 +1405,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:18:29.048528226"
+        "timestamp": "2025-05-07T13:29:36.326072757"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1431,8 +1431,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1497,10 +1497,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:15:34.335857758"
+        "timestamp": "2025-05-07T13:27:50.155561081"
     },
     "stub test all": {
         "content": [
@@ -1522,7 +1522,7 @@
                     "ctat-splicing": "0.0.2"
                 },
                 "FASTP": {
-                    "fastp": "0.23.4"
+                    "fastp": "0.24.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1541,10 +1541,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "2.1.5"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1553,8 +1553,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1587,15 +1587,15 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.14.0"
+                    "STAR-Fusion": "1.15.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -1951,9 +1951,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:13:24.397441064"
+        "timestamp": "2025-05-07T13:26:09.703953189"
     }
-}
+}
\ No newline at end of file

From 8de41e41debcaef7a6abc40aa6ae8bdf65bb0c72 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 14:42:33 +0200
Subject: [PATCH 501/887] Revert "update snap"

This reverts commit a29e06a830fe150e65fc841f8af0c85f06098958.
---
 tests/test_build.nf.test.snap    |  16 ++--
 tests/test_stub.nf.test.snap     | 124 +++++++++++++-------------
 tests/test_stub_bam.nf.test.snap | 148 +++++++++++++++----------------
 3 files changed, 144 insertions(+), 144 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index ab452ad38..5c5d08924 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -7,7 +7,7 @@
                     "arriba_download": "2.4.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -35,11 +35,11 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -220,7 +220,7 @@
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
-                "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
+                "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -277,9 +277,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:33:25.428259571"
+        "timestamp": "2025-04-23T10:08:10.68706064"
     }
-}
\ No newline at end of file
+}
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 4ce83763c..7495d02c4 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -13,8 +13,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -38,11 +38,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -51,7 +51,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -208,10 +208,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:03:38.544810125"
+        "timestamp": "2025-04-23T10:48:48.9071057"
     },
     "stub test stringtie": {
         "content": [
@@ -227,8 +227,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -258,7 +258,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -348,10 +348,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:04:11.405954818"
+        "timestamp": "2025-04-23T10:49:24.923946194"
     },
     "stub test arriba": {
         "content": [
@@ -373,8 +373,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -404,7 +404,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -489,10 +489,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:02:08.078812972"
+        "timestamp": "2025-04-23T10:47:08.02222829"
     },
     "stub test fusioninspector": {
         "content": [
@@ -514,10 +514,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -526,8 +526,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -555,7 +555,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -564,7 +564,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -863,10 +863,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:06:10.151871018"
+        "timestamp": "2025-04-23T10:51:45.508140056"
     },
     "stub test salmon": {
         "content": [
@@ -979,8 +979,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1005,7 +1005,7 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1014,7 +1014,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1184,10 +1184,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:02:40.610644223"
+        "timestamp": "2025-04-23T10:47:44.413622274"
     },
     "stub test fusionreport": {
         "content": [
@@ -1210,10 +1210,10 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1222,8 +1222,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1247,11 +1247,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1260,7 +1260,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1446,17 +1446,17 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:04:42.856027662"
+        "timestamp": "2025-04-23T10:50:05.389865001"
     },
     "stub test fastp": {
         "content": [
             10,
             {
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "0.23.4"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1520,10 +1520,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:08:02.208707357"
+        "timestamp": "2025-04-23T10:50:34.188288253"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1618,7 +1618,7 @@
                     "ctat-splicing": "0.0.2"
                 },
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "0.23.4"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1637,10 +1637,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1649,8 +1649,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1686,11 +1686,11 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1699,7 +1699,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -2071,9 +2071,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T14:01:35.330928392"
+        "timestamp": "2025-04-23T10:46:29.001694439"
     }
-}
\ No newline at end of file
+}
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 69575e5cd..99b8e5e31 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -13,8 +13,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -35,15 +35,15 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -181,10 +181,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:56:57.989800452"
+        "timestamp": "2025-04-23T11:16:16.851201431"
     },
     "stub test stringtie": {
         "content": [
@@ -200,8 +200,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -223,7 +223,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -297,10 +297,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:28:43.569555821"
+        "timestamp": "2025-04-23T11:16:59.945308111"
     },
     "stub test arriba": {
         "content": [
@@ -322,8 +322,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -345,7 +345,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -411,10 +411,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:26:43.570224768"
+        "timestamp": "2025-04-23T11:14:09.827324661"
     },
     "stub test fusioninspector": {
         "content": [
@@ -436,10 +436,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -448,8 +448,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -474,11 +474,11 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -761,10 +761,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:30:43.376109569"
+        "timestamp": "2025-04-23T11:20:00.302967276"
     },
     "stub test salmon": {
         "content": [
@@ -780,8 +780,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -871,10 +871,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:30:08.850942124"
+        "timestamp": "2025-04-23T11:19:13.383274003"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -896,8 +896,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -919,11 +919,11 @@
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1077,10 +1077,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:27:17.26404911"
+        "timestamp": "2025-04-23T11:14:53.085896038"
     },
     "stub test fusionreport": {
         "content": [
@@ -1103,10 +1103,10 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1115,8 +1115,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1137,15 +1137,15 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1312,17 +1312,17 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:58:03.351087941"
+        "timestamp": "2025-04-23T11:17:44.757543251"
     },
     "stub test fastp": {
         "content": [
             13,
             {
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "0.23.4"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1337,8 +1337,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1405,10 +1405,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:29:36.326072757"
+        "timestamp": "2025-04-23T11:18:29.048528226"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1431,8 +1431,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1497,10 +1497,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:27:50.155561081"
+        "timestamp": "2025-04-23T11:15:34.335857758"
     },
     "stub test all": {
         "content": [
@@ -1522,7 +1522,7 @@
                     "ctat-splicing": "0.0.2"
                 },
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "0.23.4"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1541,10 +1541,10 @@
                     "STAR-Fusion": null
                 },
                 "FUSIONREPORT": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "2.1.5"
+                    "fusion_report": "4.0.0"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1553,8 +1553,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.5.0.0",
+                    "samtools": "1.19.2"
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1587,15 +1587,15 @@
                     "samtools": 1.21
                 },
                 "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "STAR-Fusion": "1.14.0"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.21,
+                    "samtools": 1.2,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -1951,9 +1951,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
         },
-        "timestamp": "2025-05-07T13:26:09.703953189"
+        "timestamp": "2025-04-23T11:13:24.397441064"
     }
-}
\ No newline at end of file
+}

From 8d4cf869c9d637fc5517749533ec9931acb4e239 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 14:43:22 +0200
Subject: [PATCH 502/887] udpate snaps

---
 tests/test_build.nf.test.snap    |  4 +-
 tests/test_stub.nf.test.snap     | 64 +++++++++++------------
 tests/test_stub_bam.nf.test.snap | 90 ++++++++++++++++----------------
 3 files changed, 79 insertions(+), 79 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index db01143fb..ab452ad38 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -39,7 +39,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -280,6 +280,6 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T11:43:30.893014622"
+        "timestamp": "2025-05-07T14:33:25.428259571"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 4d70e895b..4ce83763c 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -13,8 +13,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -51,7 +51,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -211,7 +211,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T13:06:26.949894702"
+        "timestamp": "2025-05-07T14:03:38.544810125"
     },
     "stub test stringtie": {
         "content": [
@@ -227,8 +227,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -258,7 +258,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -348,10 +348,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:49:24.923946194"
+        "timestamp": "2025-05-07T14:04:11.405954818"
     },
     "stub test arriba": {
         "content": [
@@ -373,8 +373,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -404,7 +404,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -489,10 +489,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T10:47:08.02222829"
+        "timestamp": "2025-05-07T14:02:08.078812972"
     },
     "stub test fusioninspector": {
         "content": [
@@ -526,8 +526,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -564,7 +564,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -866,7 +866,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T13:08:57.754362685"
+        "timestamp": "2025-05-07T14:06:10.151871018"
     },
     "stub test salmon": {
         "content": [
@@ -979,8 +979,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1014,7 +1014,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1187,7 +1187,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T13:05:33.800125465"
+        "timestamp": "2025-05-07T14:02:40.610644223"
     },
     "stub test fusionreport": {
         "content": [
@@ -1222,8 +1222,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1260,7 +1260,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1449,7 +1449,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T13:07:32.131686727"
+        "timestamp": "2025-05-07T14:04:42.856027662"
     },
     "stub test fastp": {
         "content": [
@@ -1649,8 +1649,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1699,7 +1699,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -2074,6 +2074,6 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T13:04:26.194367038"
+        "timestamp": "2025-05-07T14:01:35.330928392"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index c84fb94ab..69575e5cd 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -13,8 +13,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -43,7 +43,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -184,7 +184,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T10:45:30.913775592"
+        "timestamp": "2025-05-07T13:56:57.989800452"
     },
     "stub test stringtie": {
         "content": [
@@ -200,8 +200,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -223,7 +223,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -297,10 +297,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:16:59.945308111"
+        "timestamp": "2025-05-07T13:28:43.569555821"
     },
     "stub test arriba": {
         "content": [
@@ -322,8 +322,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -345,7 +345,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -411,10 +411,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:14:09.827324661"
+        "timestamp": "2025-05-07T13:26:43.570224768"
     },
     "stub test fusioninspector": {
         "content": [
@@ -448,8 +448,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -478,7 +478,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -764,7 +764,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T10:47:54.208994663"
+        "timestamp": "2025-05-07T13:30:43.376109569"
     },
     "stub test salmon": {
         "content": [
@@ -780,8 +780,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -871,10 +871,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:19:13.383274003"
+        "timestamp": "2025-05-07T13:30:08.850942124"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -896,8 +896,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -923,7 +923,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1080,7 +1080,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T10:44:30.208342353"
+        "timestamp": "2025-05-07T13:27:17.26404911"
     },
     "stub test fusionreport": {
         "content": [
@@ -1115,8 +1115,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1145,7 +1145,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "Workflow": {
@@ -1315,7 +1315,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T10:46:25.615179804"
+        "timestamp": "2025-05-07T13:58:03.351087941"
     },
     "stub test fastp": {
         "content": [
@@ -1337,8 +1337,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1408,7 +1408,7 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T10:46:52.127766054"
+        "timestamp": "2025-05-07T13:29:36.326072757"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1431,8 +1431,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1497,10 +1497,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-23T11:15:34.335857758"
+        "timestamp": "2025-05-07T13:27:50.155561081"
     },
     "stub test all": {
         "content": [
@@ -1553,8 +1553,8 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.5.0.0",
-                    "samtools": "1.19.2"
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
                 },
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
@@ -1595,7 +1595,7 @@
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
-                    "samtools": 1.2,
+                    "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
                 "STRINGTIE_MERGE": {
@@ -1954,6 +1954,6 @@
             "nf-test": "0.8.4",
             "nextflow": "24.10.6"
         },
-        "timestamp": "2025-05-07T10:43:25.04013859"
+        "timestamp": "2025-05-07T13:26:09.703953189"
     }
 }
\ No newline at end of file

From cd35d33394348404f4f531c9641767d3c9d1fb1f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 15:19:58 +0200
Subject: [PATCH 503/887] update documentation

---
 CHANGELOG.md   |  1 +
 docs/output.md | 12 ++++++------
 2 files changed, 7 insertions(+), 6 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 83516b786..12b1ec52a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -42,6 +42,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
 - Moved strandedness determination for picard collectrnaseqmetrics into modules.config [#658](https://github.com/nf-core/rnafusion/pull/658)
 - Using option `--human_gencode_filter` while building STARFusion references for human species [#657](https://github.com/nf-core/rnafusion/pull/657)
+- Update fusionreport 4.0.0 that replaces the score of a fusion with a Fusion Indication Index [#667](https://github.com/nf-core/rnafusion/pull/667)
 
 ### Changed
 
diff --git a/docs/output.md b/docs/output.md
index c9f8170c0..2395eb624 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -253,7 +253,7 @@ Please note that fusion-report is executed from fork https://github.com/Clinical
 </details>
 
 [Fusion-report](https://github.com/matq007/fusion-report) is a tool for parsing outputs from fusion detection tools.
-The score is explained here: <https://github.com/Clinical-Genomics/fusion-report/blob/master/docs/score.md>. Summary:
+The Fusion Indication Index is explained here: <https://github.com/Clinical-Genomics/fusion-report/blob/master/docs/score.md>. Summary:
 
 The weights for databases are as follows:
 
@@ -261,18 +261,18 @@ The weights for databases are as follows:
 - MITELMAN (50)
 - FusionGDB2 (0)
 
-The score is calculated using two components:
+The Fusion Indication Index FII is calculated using two components:
 
-1. Tool Detection (80% of total score)
+1. Tool Detection (50% of total FII)
 
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 
-2. Database Hits (20% of total score)
+2. Database Hits (50% of total FII)
    - Based on database matches using weights above
    - Calculated as: (number of database hits) / (total possible database hits)
 
-Final score = (0.8 × Tool Detection Score) + (0.2 × Database Hits Score)
+Final score = (0.5 × Tool Detection Score) + (0.5 × Database Hits Score)
 
 ### Salmon
 
@@ -324,7 +324,7 @@ Vcf-collect takes as input the results of fusion-report and fusioninspector. Tha
 
 </details>
 
-[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
+[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualized in the report and further statistics are available in the report data directory.
 
 Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
 

From 3d2f4d61edd7fa313cf47c9e43dbe53a9ea9f76c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 15:21:11 +0200
Subject: [PATCH 504/887] update changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 34e3932c7..9824ccfc8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -42,7 +42,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added the `seq_platform` and `seq_center` fields to the samplesheet. These values can be used to overwrite the value of `--seq_platform` and `--seq_center` on a sample-by-sample basis [#654](https://github.com/nf-core/rnafusion/pull/654)
 - Moved strandedness determination for picard collectrnaseqmetrics into modules.config [#658](https://github.com/nf-core/rnafusion/pull/658)
 - Using option `--human_gencode_filter` while building STARFusion references for human species [#657](https://github.com/nf-core/rnafusion/pull/657)
-- Update STAR-Fusion to 1.15.0 and using human filtering when species is human [#664](https://github.com/nf-core/rnafusion/pull/664)
+- Update STAR-Fusion to 1.15.0 and update nf-core modules [#664](https://github.com/nf-core/rnafusion/pull/664)
 
 ### Changed
 

From d33f48e71b005d237bfcb7999427b30f0194b86d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 16:05:37 +0200
Subject: [PATCH 505/887] add Pfam version parameter

---
 modules/local/starfusion/build/main.nf      | 3 ++-
 modules/local/starfusion/build/meta.yml     | 3 +++
 nextflow.config                             | 1 +
 nextflow_schema.json                        | 4 ++++
 subworkflows/local/build_references/main.nf | 2 +-
 5 files changed, 11 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 0d7ab3230..daf296f33 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -13,6 +13,7 @@ process STARFUSION_BUILD {
     path fusion_annot_lib
     val dfam_species
     val dfam_version
+    val pfam_version
 
     output:
     tuple val(meta), path("ctat_genome_lib_build_dir"), emit: reference
@@ -21,7 +22,7 @@ process STARFUSION_BUILD {
     script:
     def args = task.ext.args ?: ''
     """
-    wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz --no-check-certificate
+    wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${pfam_version}/Pfam-A.hmm.gz --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3f --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3i --no-check-certificate
diff --git a/modules/local/starfusion/build/meta.yml b/modules/local/starfusion/build/meta.yml
index 1259f2698..1294e730e 100644
--- a/modules/local/starfusion/build/meta.yml
+++ b/modules/local/starfusion/build/meta.yml
@@ -30,6 +30,9 @@ input:
   - dfam_version:
       type: string
       description: DNA transposable element database version, required for repeat masking. Values such as '3.4' are accepted (will automatically pull the resources from dfam).
+  - dfam_version:
+      type: string
+      description: Protein families database version, to get domain information. Values such as '37.4' are accepted (will automatically pull the resources from Pfam).
 
 output:
   - reference:
diff --git a/nextflow.config b/nextflow.config
index 60e9c9815..b89336acd 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -33,6 +33,7 @@ params {
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
     species                    = "homo_sapiens"
     dfam_version               = '3.8'
+    pfam_version               = '37.4'
 
     // Filtering
     tools_cutoff               = 1
diff --git a/nextflow_schema.json b/nextflow_schema.json
index be565ef1f..84cf98575 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -364,6 +364,10 @@
                 "dfam_version": {
                     "type": "string",
                     "description": "Version of dfam to use"
+                },
+                "pfam_version": {
+                    "type": "string",
+                    "description": "Version of pfam to use"
                 }
             }
         },
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 1767a6123..6858cf894 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -171,7 +171,7 @@ workflow BUILD_REFERENCES {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
             }
-            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, params.dfam_version)
+            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, params.dfam_version, params.pfam_version)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
                 CTATSPLICING_PREPGENOMELIB(

From e5025e4e5e749a4edb951d4059c6f51c0690ab05 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 7 May 2025 20:45:48 +0200
Subject: [PATCH 506/887] update snapshots for module

---
 modules/local/starfusion/build/meta.yml       |  2 +-
 .../local/starfusion/build/tests/main.nf.test |  6 +++--
 .../starfusion/build/tests/main.nf.test.snap  | 22 +++++++++----------
 3 files changed, 16 insertions(+), 14 deletions(-)

diff --git a/modules/local/starfusion/build/meta.yml b/modules/local/starfusion/build/meta.yml
index 1294e730e..bfa0a025a 100644
--- a/modules/local/starfusion/build/meta.yml
+++ b/modules/local/starfusion/build/meta.yml
@@ -30,7 +30,7 @@ input:
   - dfam_version:
       type: string
       description: DNA transposable element database version, required for repeat masking. Values such as '3.4' are accepted (will automatically pull the resources from dfam).
-  - dfam_version:
+  - pfam_version:
       type: string
       description: Protein families database version, to get domain information. Values such as '37.4' are accepted (will automatically pull the resources from Pfam).
 
diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
index 7a98b8e7c..07777230e 100644
--- a/modules/local/starfusion/build/tests/main.nf.test
+++ b/modules/local/starfusion/build/tests/main.nf.test
@@ -20,7 +20,8 @@ nextflow_process {
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
                 input [3] = "homo_sapiens"
-                input [4] = "3.4"
+                input [4] = "3.8"
+                input [5] = "37.4"
 
                 """
             }
@@ -125,7 +126,8 @@ nextflow_process {
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
                 input [3] = "homo_sapiens"
-                input [4] = "3.4"
+                input [4] = "3.8"
+                input [5] = "37.4"
 
                 """
             }
diff --git a/modules/local/starfusion/build/tests/main.nf.test.snap b/modules/local/starfusion/build/tests/main.nf.test.snap
index b647347a1..3e6358d68 100644
--- a/modules/local/starfusion/build/tests/main.nf.test.snap
+++ b/modules/local/starfusion/build/tests/main.nf.test.snap
@@ -1,7 +1,7 @@
 {
     "STARFUSION_BUILD - human - minigenome": {
         "content": [
-            "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
+            "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
             true,
             true,
             "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -75,14 +75,14 @@
             true,
             "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
             [
-                "versions.yml:md5,4a6ed47cb345f2a5af4d9f12b7918cdb"
+                "versions.yml:md5,99533491facb961016dafc1d45a8101e"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-15T17:34:00.624179533"
+        "timestamp": "2025-05-07T20:31:12.177376155"
     },
     "STARFUSION_BUILD - human - minigenome - stub": {
         "content": [
@@ -173,7 +173,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,4a6ed47cb345f2a5af4d9f12b7918cdb"
+                    "versions.yml:md5,99533491facb961016dafc1d45a8101e"
                 ],
                 "reference": [
                     [
@@ -261,14 +261,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,4a6ed47cb345f2a5af4d9f12b7918cdb"
+                    "versions.yml:md5,99533491facb961016dafc1d45a8101e"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nf-test": "0.8.4",
+            "nextflow": "24.10.6"
         },
-        "timestamp": "2025-04-15T17:34:36.490161525"
+        "timestamp": "2025-05-07T20:31:53.437179641"
     }
-}
+}
\ No newline at end of file

From 1d61985f30a26d46b16d1e6c68fe922df02a7e5d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 8 May 2025 13:47:50 +0200
Subject: [PATCH 507/887] update fusioninspector containers

---
 modules/local/fusioninspector/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index d89f252ed..dc3e5f692 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/13/139b94a1f10c6e23a8c27eaed1e5a689db978a513d0ee155e74d35f0970814fe/data' :
-        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:c6147971d107ab11'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)

From 024a4d01b18602198ef8075de46200c02e529042 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 15 May 2025 12:44:05 +0200
Subject: [PATCH 508/887] update starfusion docker container

---
 modules/local/starfusion/build/main.nf  | 2 +-
 modules/local/starfusion/detect/main.nf | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index daf296f33..5acf81def 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -5,7 +5,7 @@ process STARFUSION_BUILD {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c1af244ebcf603cf'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 0bf271541..3b71c194b 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -5,7 +5,7 @@ process STARFUSION {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c1af244ebcf603cf'}"
 
     input:
     tuple val(meta), path(reads), path(junction)

From fcd5418f0518672a7ada43963f0eef2a42bf9851 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 15 May 2025 12:47:25 +0200
Subject: [PATCH 509/887] revert change

---
 modules/local/fusioninspector/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index dc3e5f692..d89f252ed 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -4,8 +4,8 @@ process FUSIONINSPECTOR {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/13/139b94a1f10c6e23a8c27eaed1e5a689db978a513d0ee155e74d35f0970814fe/data' :
+        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:c6147971d107ab11'}"
 
     input:
     tuple val(meta), path(reads), path(fusion_list)

From 9ac700f05b5e485c21289f7b681fa7673173955b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 15 May 2025 12:49:41 +0200
Subject: [PATCH 510/887] fix arguments for fusioninspector

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index deb68ef05..1fb9675b4 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -103,7 +103,7 @@ process {
         ]
     }
 
-    withName: '.*FUSIONINSPECTOR_WORKFLOW:.*:FUSIONINSPECTOR' {
+    withName: 'FUSIONINSPECTOR' {
         ext.args = { params.fusioninspector_limitSjdbInsertNsj != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
         ext.args2 = '--annotate --examine_coding_effect'
     }

From efce041ebb2366f8704f39b22a34d4d07b6b1c1a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 15 May 2025 13:09:24 +0200
Subject: [PATCH 511/887] update changelog

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1446e4127..f3bb09357 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -80,6 +80,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `fusioninspector`
   - `all` => This will automatically run all of the above tools
 - Updated all `wget` containers to `conda-forge::wget=1.21.4` [#655](https://github.com/nf-core/rnafusion/pull/655)
+- Updated STAR-Fusion container for version 1.15 [#669](https://github.com/nf-core/rnafusion/pull/669)
+- Fix bug in argument handling for FusionInspector [#669](https://github.com/nf-core/rnafusion/pull/669)
 
 ### Fixed
 

From a524ab1239eeff38688e5734c7d7393efa5802e9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 15 May 2025 13:16:33 +0200
Subject: [PATCH 512/887] update docker star container

---
 modules/local/starfusion/build/main.nf  | 2 +-
 modules/local/starfusion/detect/main.nf | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 5acf81def..daf296f33 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -5,7 +5,7 @@ process STARFUSION_BUILD {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c1af244ebcf603cf'}"
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 3b71c194b..0bf271541 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -5,7 +5,7 @@ process STARFUSION {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c1af244ebcf603cf'}"
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
 
     input:
     tuple val(meta), path(reads), path(junction)

From dc52347007b8339917514ab51f2899e3c48252a8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 19 May 2025 13:55:45 +0200
Subject: [PATCH 513/887] slight update in tests

---
 modules/local/fusioninspector/tests/main.nf.test      | 8 +++++---
 modules/local/fusioninspector/tests/main.nf.test.snap | 4 ++--
 2 files changed, 7 insertions(+), 5 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 26a7ca6d9..1b0a837e8 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -25,7 +25,9 @@ nextflow_process {
                     ]
 
                     input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                    input[3] = "human"
+                    input[3] = "homo_sapiens"
+                    input [4] = "3.8"
+                    input [5] = "37.4"
                     """
                 }
             }
@@ -44,7 +46,7 @@ nextflow_process {
                         file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
                     ]
                 ]
-                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
+                input[1] = [[id:'minigenome_refs'],[STARFUSION_BUILD.out.reference.map { it[1] }]]
                 """
             }
         }
@@ -128,7 +130,7 @@ nextflow_process {
                         file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
                     ]
                 ]
-                input[1] = []
+                input[1] = [[id:'minigenome_refs'],[]]
                 """
             }
         }
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 6e3f05c2c..7f7749306 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -380,9 +380,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2024-12-31T13:48:13.020571472"
+        "timestamp": "2025-05-16T17:16:34.451066"
     },
     "FUSIONINSPECTOR": {
         "content": [

From 8f0884ab7ecd78c6a97f942a1a7b4bd6271f7826 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 19 May 2025 14:10:16 +0200
Subject: [PATCH 514/887] abridged now contains the coding effect

---
 modules/local/fusioninspector/main.nf          | 1 -
 subworkflows/local/fusioninspector_workflow.nf | 2 +-
 2 files changed, 1 insertion(+), 2 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index d89f252ed..7f1f18278 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -13,7 +13,6 @@ process FUSIONINSPECTOR {
 
     output:
     tuple val(meta), path("*FusionInspector.fusions.tsv")                  , emit: tsv
-    tuple val(meta), path("*.coding_effect")                , optional:true, emit: tsv_coding_effect
     tuple val(meta), path("*.gtf")                          , optional:true, emit: out_gtf
     tuple val(meta), path("*FusionInspector.log")                          , emit: log
     tuple val(meta), path("*html")                                         , emit: html
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index 754be50cc..f33d2a86f 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -46,7 +46,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             AGAT_CONVERTSPGFF2TSV(FUSIONINSPECTOR.out.out_gtf)
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
-            fusion_data = FUSIONINSPECTOR.out.tsv_coding_effect.join(AGAT_CONVERTSPGFF2TSV.out.tsv).join(fusionreport_out).join(fusionreport_csv)
+            fusion_data = FUSIONINSPECTOR.out.abridged_tsv.join(AGAT_CONVERTSPGFF2TSV.out.tsv).join(fusionreport_out).join(fusionreport_csv)
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)

From 196297a2ebd7de7c8b72f45f927425e0f2e7be20 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 21 May 2025 16:47:02 +0200
Subject: [PATCH 515/887] update test

---
 .../local/fusioninspector/tests/main.nf.test  |  2 +-
 .../fusioninspector/tests/main.nf.test.snap   | 20 +++++++------------
 .../fusioninspector/tests/nextflow.config     |  3 +--
 3 files changed, 9 insertions(+), 16 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 1b0a837e8..529b14cc5 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -46,7 +46,7 @@ nextflow_process {
                         file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
                     ]
                 ]
-                input[1] = [[id:'minigenome_refs'],[STARFUSION_BUILD.out.reference.map { it[1] }]]
+                input[1] = STARFUSION_BUILD.out.reference
                 """
             }
         }
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 7f7749306..20c0e710d 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -15,9 +15,6 @@
                     
                 ],
                 "2": [
-                    
-                ],
-                "3": [
                     [
                         {
                             "id": "test",
@@ -26,7 +23,7 @@
                         "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "4": [
+                "3": [
                     [
                         {
                             "id": "test",
@@ -35,7 +32,7 @@
                         "test.fusion_inspector_web.html:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "5": [
+                "4": [
                     [
                         {
                             "id": "test",
@@ -44,7 +41,7 @@
                         "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "6": [
+                "5": [
                     [
                         {
                             "id": "test",
@@ -73,7 +70,7 @@
                         ]
                     ]
                 ],
-                "7": [
+                "6": [
                     [
                         {
                             "id": "test",
@@ -133,7 +130,7 @@
                         ]
                     ]
                 ],
-                "8": [
+                "7": [
                     [
                         {
                             "id": "test",
@@ -186,7 +183,7 @@
                         ]
                     ]
                 ],
-                "9": [
+                "8": [
                     "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
                 ],
                 "abridged_tsv": [
@@ -369,9 +366,6 @@
                         },
                         "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
-                ],
-                "tsv_coding_effect": [
-                    
                 ],
                 "versions": [
                     "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
@@ -382,7 +376,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-16T17:16:34.451066"
+        "timestamp": "2025-05-19T14:11:46.597061"
     },
     "FUSIONINSPECTOR": {
         "content": [
diff --git a/modules/local/fusioninspector/tests/nextflow.config b/modules/local/fusioninspector/tests/nextflow.config
index ca61431b2..1cc66c43e 100644
--- a/modules/local/fusioninspector/tests/nextflow.config
+++ b/modules/local/fusioninspector/tests/nextflow.config
@@ -1,5 +1,4 @@
 process {
     withName: 'FUSIONINSPECTOR' {
-        ext.args = '--annotate --examine_coding_effect'
-    }
+        ext.args = '--annotate --examine_coding_effect'    }
 }

From ef9c99956fb1932280327f540c4f3289fd34f336 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 21 May 2025 16:58:31 +0200
Subject: [PATCH 516/887] update changelog

---
 CHANGELOG.md | 5 +++--
 1 file changed, 3 insertions(+), 2 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f3bb09357..7e4688cc7 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -80,8 +80,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `fusioninspector`
   - `all` => This will automatically run all of the above tools
 - Updated all `wget` containers to `conda-forge::wget=1.21.4` [#655](https://github.com/nf-core/rnafusion/pull/655)
-- Updated STAR-Fusion container for version 1.15 [#669](https://github.com/nf-core/rnafusion/pull/669)
-- Fix bug in argument handling for FusionInspector [#669](https://github.com/nf-core/rnafusion/pull/669)
+- Using FusionInspector abridged output (that now contains coding effects) for downstream analysis [#669](https://github.com/nf-core/rnafusion/pull/669)
 
 ### Fixed
 
@@ -97,6 +96,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated the input validation to be more strict. This will prevent more errors down the line in the pipeline [#640](https://github.com/nf-core/rnafusion/pull/640)
 - The `FUSIONINSPECTOR` process will no longer fail when no fusions have been found. [#651](https://github.com/nf-core/rnafusion/pull/651)
 - Fixed STAR-Fusion build would fail when downloading Pfam and Dfam resources behind SSL. [#653](https://github.com/nf-core/rnafusion/pull/653)
+- Fix bug in argument handling for FusionInspector [#669](https://github.com/nf-core/rnafusion/pull/669)
+
 
 ### Removed
 

From 3aa8243e1670325b9cf31ea8abd1c9b727b5ffa1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 21 May 2025 17:06:54 +0200
Subject: [PATCH 517/887] prettier

---
 CHANGELOG.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7e4688cc7..94c5a7d6b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -98,7 +98,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed STAR-Fusion build would fail when downloading Pfam and Dfam resources behind SSL. [#653](https://github.com/nf-core/rnafusion/pull/653)
 - Fix bug in argument handling for FusionInspector [#669](https://github.com/nf-core/rnafusion/pull/669)
 
-
 ### Removed
 
 - Remove fusionGDB from documentation and fusion-report download stubs [#503](https://github.com/nf-core/rnafusion/pull/503)

From 2c9f4b9a19429057996462275979a03a18961f80 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 28 May 2025 13:47:36 +0200
Subject: [PATCH 518/887] add trim_tail_fusioncatcher

---
 conf/modules.config                              |  4 ++++
 nextflow.config                                  |  1 +
 nextflow_schema.json                             |  6 ++++++
 .../local/fusioncatcher_workflow/main.nf         | 16 +++++++++++++++-
 workflows/rnafusion.nf                           |  2 ++
 5 files changed, 28 insertions(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index deb68ef05..5e5a3a3f0 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -68,6 +68,10 @@ process {
         ext.args = { params.trim_tail ? "--trim_tail1 ${params.trim_tail} --trim_tail2 ${params.trim_tail} " : '' }
     }
 
+    withName: 'FASTP_FOR_FUSIONCATCHER' {
+        ext.args = { params.trim_tail_fusioncatcher ? "--trim_tail1 ${params.trim_tail_fusioncatcher} --trim_tail2 ${params.trim_tail_fusioncatcher} " : '' }
+    }
+
     withName: 'FASTQC' {
         ext.args = '--quiet'
         publishDir = [
diff --git a/nextflow.config b/nextflow.config
index d46ef7e22..1c22ae581 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -40,6 +40,7 @@ params {
 
     // Trimming
     trim_tail                  = null
+    trim_tail_fusioncatcher    = null
     adapter_fasta              = []
 
     // Compression
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 99a45894c..2ee1b201c 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -266,6 +266,12 @@
                     "minimum": 1,
                     "fa_icon": "fas fa-cut"
                 },
+                "trim_tail_fusioncatcher": {
+                    "type": "integer",
+                    "description": "The amount of bases to trim at the tail of each read for fusioncatcher, none will be trimmed by default",
+                    "minimum": 1,
+                    "fa_icon": "fas fa-cut"
+                },
                 "adapter_fasta": {
                     "type": "string",
                     "format": "file-path",
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index f4f053d2f..b2e5fadd2 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -1,4 +1,5 @@
-include { FUSIONCATCHER_FUSIONCATCHER } from '../../../modules/nf-core/fusioncatcher/fusioncatcher/main'
+include { FASTP as FASTP_FOR_FUSIONCATCHER } from '../../../modules/nf-core/fastp/main'
+include { FUSIONCATCHER_FUSIONCATCHER }      from '../../../modules/nf-core/fusioncatcher/fusioncatcher/main'
 
 // TODO: Remove fusioncatcher_fusions as parameter.
 // TODO: remove dummy file. Work with Channel.empty()
@@ -7,6 +8,8 @@ include { FUSIONCATCHER_FUSIONCATCHER } from '../../../modules/nf-core/fusioncat
 workflow FUSIONCATCHER_WORKFLOW {
     take:
         reads                   // channel [ meta, [ fastqs ] ]
+        fusioncatcher_trim     // boolean
+        adapter_fasta         // channel [ path ]
         fusioncatcher_ref       // channel [ meta, path       ]
         fusioncatcher_fusions   // path, string
 
@@ -18,6 +21,17 @@ workflow FUSIONCATCHER_WORKFLOW {
             ch_fusioncatcher_fusions = reads.combine(Channel.value(file(fusioncatcher_fusions, checkIfExists:true)))
                                         .map { meta, _reads, fusions -> [ meta, fusions ] }
         } else {
+            if (fusioncatcher_trim) {
+                FASTP_FOR_FUSIONCATCHER(
+                    reads,
+                    adapter_fasta,
+                    false, // discard_trimmed_pass
+                    false, // save_trimmed_fail
+                    false  // skip_qc
+                )
+                ch_versions = ch_versions.mix(FASTP_FOR_FUSIONCATCHER.out.versions)
+                reads = FASTP_FOR_FUSIONCATCHER.out.reads
+            }
 
             FUSIONCATCHER_FUSIONCATCHER (
                 reads,
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bda6500af..d9f8d9230 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -257,6 +257,8 @@ workflow RNAFUSION {
             fusions_created = true
             FUSIONCATCHER_WORKFLOW (
                 ch_reads,
+                params.tools?.contains('sth') ?: false,
+                params.adapter_fasta,
                 BUILD_REFERENCES.out.fusioncatcher_ref,       // channel [ meta, path       ]
                 params.fusioncatcher_fusions
             )

From 6690c15ef108df77763835c3d474a832a5f0450d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 28 May 2025 14:23:51 +0200
Subject: [PATCH 519/887] add fusioncatcher_trim option

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index d9f8d9230..2f5dc15fe 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -257,7 +257,7 @@ workflow RNAFUSION {
             fusions_created = true
             FUSIONCATCHER_WORKFLOW (
                 ch_reads,
-                params.tools?.contains('sth') ?: false,
+                params.tools?.contains('fusioncatcher_trim') ?: false,
                 params.adapter_fasta,
                 BUILD_REFERENCES.out.fusioncatcher_ref,       // channel [ meta, path       ]
                 params.fusioncatcher_fusions

From a7da82c9995b9b896c2b63ccbf4f4a62c264686a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 28 May 2025 15:40:57 +0200
Subject: [PATCH 520/887] add fusioncatcher_trim

---
 nextflow_schema.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 2ee1b201c..56f55d755 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -85,7 +85,7 @@
                     "type": "string",
                     "description": "Comma-delimited list of tools to run",
                     "fa_icon": "far fa-file-code",
-                    "pattern": "^((arriba|ctatsplicing|fusioncatcher|starfusion|stringtie|fusionreport|fastp|salmon|fusioninspector|all)?,?)*(?<!,)$"
+                    "pattern": "^((arriba|ctatsplicing|fusioncatcher|fusioncatcher_trim|starfusion|stringtie|fusionreport|fastp|salmon|fusioninspector|all)?,?)*(?<!,)$"
                 },
                 "read_length": {
                     "type": "integer",

From 87601764045d63051cbcce2524fe37e4bc7710db Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 28 May 2025 16:00:28 +0200
Subject: [PATCH 521/887] add fusioncatcher_trim

---
 workflows/rnafusion.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2f5dc15fe..13a6ea157 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -255,9 +255,10 @@ workflow RNAFUSION {
         def ch_fusioncatcher_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
             fusions_created = true
+            fusioncatcher_trimming = { params.tools?.contains('fusioncatcher_trim') ?: false }
             FUSIONCATCHER_WORKFLOW (
                 ch_reads,
-                params.tools?.contains('fusioncatcher_trim') ?: false,
+                fusioncatcher_trimming,
                 params.adapter_fasta,
                 BUILD_REFERENCES.out.fusioncatcher_ref,       // channel [ meta, path       ]
                 params.fusioncatcher_fusions

From 8ab02d8f9a54b1de461475bf2b5d83c3ac207a06 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 2 Jun 2025 10:43:36 +0200
Subject: [PATCH 522/887] add publish

---
 conf/modules.config | 10 ++++++++++
 1 file changed, 10 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 5e5a3a3f0..6dd391019 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -66,10 +66,20 @@ process {
 
     withName: 'FASTP' {
         ext.args = { params.trim_tail ? "--trim_tail1 ${params.trim_tail} --trim_tail2 ${params.trim_tail} " : '' }
+        publishDir = [
+            path: { "${params.outdir}/fastp" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
 
     withName: 'FASTP_FOR_FUSIONCATCHER' {
         ext.args = { params.trim_tail_fusioncatcher ? "--trim_tail1 ${params.trim_tail_fusioncatcher} --trim_tail2 ${params.trim_tail_fusioncatcher} " : '' }
+        publishDir = [
+            path: { "${params.outdir}/fastp_for_fusioncatcher" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
 
     withName: 'FASTQC' {

From 680e03d87f8b1ec6002f9f9ec94fd4de46510697 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 2 Jun 2025 11:20:04 +0200
Subject: [PATCH 523/887] add differentiating prefix

---
 conf/modules.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/modules.config b/conf/modules.config
index 98eb8e89e..17e798cf7 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -75,6 +75,7 @@ process {
 
     withName: 'FASTP_FOR_FUSIONCATCHER' {
         ext.args = { params.trim_tail_fusioncatcher ? "--trim_tail1 ${params.trim_tail_fusioncatcher} --trim_tail2 ${params.trim_tail_fusioncatcher} " : '' }
+        ext.prefix = { "${meta.id}_trimmed_for_fusioncatcher" }
         publishDir = [
             path: { "${params.outdir}/fastp_for_fusioncatcher" },
             mode: params.publish_dir_mode,

From f630a2bc4a27d09c4a516d218169265fb0f02b51 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 2 Jun 2025 14:59:46 +0200
Subject: [PATCH 524/887] try changing the condition

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 13a6ea157..ee970fe9f 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -255,7 +255,7 @@ workflow RNAFUSION {
         def ch_fusioncatcher_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
             fusions_created = true
-            fusioncatcher_trimming = { params.tools?.contains('fusioncatcher_trim') ?: false }
+            fusioncatcher_trimming = { params.tools.contains('fusioncatcher_trim') ?: false }
             FUSIONCATCHER_WORKFLOW (
                 ch_reads,
                 fusioncatcher_trimming,

From 37104665e78333a4a0535b6acd344acfada4ef36 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 3 Jun 2025 11:02:43 +0000
Subject: [PATCH 525/887] Template update for nf-core/tools version 3.3.1

---
 .editorconfig                                 |  37 -----
 .github/CONTRIBUTING.md                       |   2 +-
 .github/actions/get-shards/action.yml         |  69 +++++++++
 .github/actions/nf-test/action.yml            | 113 ++++++++++++++
 .github/workflows/awsfulltest.yml             |   4 +-
 .github/workflows/awstest.yml                 |   2 +-
 .github/workflows/ci.yml                      |  88 -----------
 .github/workflows/clean-up.yml                |   2 +-
 .github/workflows/download_pipeline.yml       |  20 +--
 .../{fix-linting.yml => fix_linting.yml}      |   4 +-
 .github/workflows/linting.yml                 |  15 +-
 .github/workflows/linting_comment.yml         |   4 +-
 .github/workflows/nf-test.yml                 | 142 ++++++++++++++++++
 .github/workflows/release-announcements.yml   |   2 +-
 ...mment.yml => template-version-comment.yml} |   2 +-
 .nf-core.yml                                  |   2 +-
 .pre-commit-config.yaml                       |  26 +++-
 .prettierrc.yml                               |   5 +
 README.md                                     |   7 +-
 conf/base.config                              |   5 +-
 nextflow.config                               |  17 ++-
 nf-test.config                                |  24 +++
 ro-crate-metadata.json                        |  16 +-
 .../utils_nfcore_rnafusion_pipeline/main.nf   |   1 -
 tests/.nftignore                              |  10 ++
 tests/default.nf.test                         |  35 +++++
 tests/nextflow.config                         |  12 ++
 27 files changed, 487 insertions(+), 179 deletions(-)
 delete mode 100644 .editorconfig
 create mode 100644 .github/actions/get-shards/action.yml
 create mode 100644 .github/actions/nf-test/action.yml
 delete mode 100644 .github/workflows/ci.yml
 rename .github/workflows/{fix-linting.yml => fix_linting.yml} (96%)
 create mode 100644 .github/workflows/nf-test.yml
 rename .github/workflows/{template_version_comment.yml => template-version-comment.yml} (95%)
 create mode 100644 nf-test.config
 create mode 100644 tests/.nftignore
 create mode 100644 tests/default.nf.test
 create mode 100644 tests/nextflow.config

diff --git a/.editorconfig b/.editorconfig
deleted file mode 100644
index 6d9b74cc0..000000000
--- a/.editorconfig
+++ /dev/null
@@ -1,37 +0,0 @@
-root = true
-
-[*]
-charset = utf-8
-end_of_line = lf
-insert_final_newline = true
-trim_trailing_whitespace = true
-indent_size = 4
-indent_style = space
-
-[*.{md,yml,yaml,html,css,scss,js}]
-indent_size = 2
-
-# These files are edited and tested upstream in nf-core/modules
-[/modules/nf-core/**]
-charset = unset
-end_of_line = unset
-insert_final_newline = unset
-trim_trailing_whitespace = unset
-indent_style = unset
-[/subworkflows/nf-core/**]
-charset = unset
-end_of_line = unset
-insert_final_newline = unset
-trim_trailing_whitespace = unset
-indent_style = unset
-
-[/assets/email*]
-indent_size = unset
-
-# ignore python and markdown
-[*.{py,md}]
-indent_style = unset
-
-# ignore ro-crate metadata files
-[**/ro-crate-metadata.json]
-insert_final_newline = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index b10a5a24f..96b68fa8a 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -78,7 +78,7 @@ If you wish to contribute a new step, please use the following coding standards:
 5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool).
 6. Add sanity checks and validation for all relevant parameters.
 7. Perform local tests to validate that the new code works as expected.
-8. If applicable, add a new test command in `.github/workflow/ci.yml`.
+8. If applicable, add a new test in the `tests` directory.
 9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
 10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
 
diff --git a/.github/actions/get-shards/action.yml b/.github/actions/get-shards/action.yml
new file mode 100644
index 000000000..34085279f
--- /dev/null
+++ b/.github/actions/get-shards/action.yml
@@ -0,0 +1,69 @@
+name: "Get number of shards"
+description: "Get the number of nf-test shards for the current CI job"
+inputs:
+  max_shards:
+    description: "Maximum number of shards allowed"
+    required: true
+  paths:
+    description: "Component paths to test"
+    required: false
+  tags:
+    description: "Tags to pass as argument for nf-test --tag parameter"
+    required: false
+outputs:
+  shard:
+    description: "Array of shard numbers"
+    value: ${{ steps.shards.outputs.shard }}
+  total_shards:
+    description: "Total number of shards"
+    value: ${{ steps.shards.outputs.total_shards }}
+runs:
+  using: "composite"
+  steps:
+    - name: Install nf-test
+      uses: nf-core/setup-nf-test@v1
+      with:
+        version: ${{ env.NFT_VER }}
+    - name: Get number of shards
+      id: shards
+      shell: bash
+      run: |
+        # Run nf-test with dynamic parameter
+        nftest_output=$(nf-test test \
+          --profile +docker \
+          $(if [ -n "${{ inputs.tags }}" ]; then echo "--tag ${{ inputs.tags }}"; fi) \
+          --dry-run \
+          --ci \
+          --changed-since HEAD^) || {
+            echo "nf-test command failed with exit code $?"
+            echo "Full output: $nftest_output"
+            exit 1
+        }
+        echo "nf-test dry-run output: $nftest_output"
+
+        # Default values for shard and total_shards
+        shard="[]"
+        total_shards=0
+
+        # Check if there are related tests
+        if echo "$nftest_output" | grep -q 'No tests to execute'; then
+          echo "No related tests found."
+        else
+          # Extract the number of related tests
+          number_of_shards=$(echo "$nftest_output" | sed -n 's|.*Executed \([0-9]*\) tests.*|\1|p')
+          if [[ -n "$number_of_shards" && "$number_of_shards" -gt 0 ]]; then
+            shards_to_run=$(( $number_of_shards < ${{ inputs.max_shards }} ? $number_of_shards : ${{ inputs.max_shards }} ))
+            shard=$(seq 1 "$shards_to_run" | jq -R . | jq -c -s .)
+            total_shards="$shards_to_run"
+          else
+            echo "Unexpected output format. Falling back to default values."
+          fi
+        fi
+
+        # Write to GitHub Actions outputs
+        echo "shard=$shard" >> $GITHUB_OUTPUT
+        echo "total_shards=$total_shards" >> $GITHUB_OUTPUT
+
+        # Debugging output
+        echo "Final shard array: $shard"
+        echo "Total number of shards: $total_shards"
diff --git a/.github/actions/nf-test/action.yml b/.github/actions/nf-test/action.yml
new file mode 100644
index 000000000..243e78238
--- /dev/null
+++ b/.github/actions/nf-test/action.yml
@@ -0,0 +1,113 @@
+name: "nf-test Action"
+description: "Runs nf-test with common setup steps"
+inputs:
+  profile:
+    description: "Profile to use"
+    required: true
+  shard:
+    description: "Shard number for this CI job"
+    required: true
+  total_shards:
+    description: "Total number of test shards(NOT the total number of matrix jobs)"
+    required: true
+  paths:
+    description: "Test paths"
+    required: true
+  tags:
+    description: "Tags to pass as argument for nf-test --tag parameter"
+    required: false
+runs:
+  using: "composite"
+  steps:
+    - name: Setup Nextflow
+      uses: nf-core/setup-nextflow@v2
+      with:
+        version: "${{ env.NXF_VERSION }}"
+
+    - name: Set up Python
+      uses: actions/setup-python@a26af69be951a213d495a4c3e4e4022e16d87065 # v5
+      with:
+        python-version: "3.13"
+
+    - name: Install nf-test
+      uses: nf-core/setup-nf-test@v1
+      with:
+        version: "${{ env.NFT_VER }}"
+        install-pdiff: true
+
+    - name: Setup apptainer
+      if: contains(inputs.profile, 'singularity')
+      uses: eWaterCycle/setup-apptainer@main
+
+    - name: Set up Singularity
+      if: contains(inputs.profile, 'singularity')
+      shell: bash
+      run: |
+        mkdir -p $NXF_SINGULARITY_CACHEDIR
+        mkdir -p $NXF_SINGULARITY_LIBRARYDIR
+
+    - name: Conda setup
+      if: contains(inputs.profile, 'conda')
+      uses: conda-incubator/setup-miniconda@505e6394dae86d6a5c7fbb6e3fb8938e3e863830 # v3
+      with:
+        auto-update-conda: true
+        conda-solver: libmamba
+        conda-remove-defaults: true
+
+      # TODO Skip failing conda tests and document their failures
+      # https://github.com/nf-core/modules/issues/7017
+    - name: Run nf-test
+      shell: bash
+      env:
+        NFT_DIFF: ${{ env.NFT_DIFF }}
+        NFT_DIFF_ARGS: ${{ env.NFT_DIFF_ARGS }}
+        NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
+      run: |
+        nf-test test \
+          --profile=+${{ inputs.profile }} \
+          $(if [ -n "${{ inputs.tags }}" ]; then echo "--tag ${{ inputs.tags }}"; fi) \
+          --ci \
+          --changed-since HEAD^ \
+          --verbose \
+          --tap=test.tap \
+          --shard ${{ inputs.shard }}/${{ inputs.total_shards }}
+
+          # Save the absolute path of the test.tap file to the output
+          echo "tap_file_path=$(realpath test.tap)" >> $GITHUB_OUTPUT
+
+    - name: Generate test summary
+      if: always()
+      shell: bash
+      run: |
+        # Add header if it doesn't exist (using a token file to track this)
+        if [ ! -f ".summary_header" ]; then
+          echo "# 🚀 nf-test results" >> $GITHUB_STEP_SUMMARY
+          echo "" >> $GITHUB_STEP_SUMMARY
+          echo "| Status | Test Name | Profile | Shard |" >> $GITHUB_STEP_SUMMARY
+          echo "|:------:|-----------|---------|-------|" >> $GITHUB_STEP_SUMMARY
+          touch .summary_header
+        fi
+
+        if [ -f test.tap ]; then
+          while IFS= read -r line; do
+            if [[ $line =~ ^ok ]]; then
+              test_name="${line#ok }"
+              # Remove the test number from the beginning
+              test_name="${test_name#* }"
+              echo "| ✅ | ${test_name} | ${{ inputs.profile }} | ${{ inputs.shard }}/${{ inputs.total_shards }} |" >> $GITHUB_STEP_SUMMARY
+            elif [[ $line =~ ^not\ ok ]]; then
+              test_name="${line#not ok }"
+              # Remove the test number from the beginning
+              test_name="${test_name#* }"
+              echo "| ❌ | ${test_name} | ${{ inputs.profile }} | ${{ inputs.shard }}/${{ inputs.total_shards }} |" >> $GITHUB_STEP_SUMMARY
+            fi
+          done < test.tap
+        else
+          echo "| ⚠️ | No test results found | ${{ inputs.profile }} | ${{ inputs.shard }}/${{ inputs.total_shards }} |" >> $GITHUB_STEP_SUMMARY
+        fi
+
+    - name: Clean up
+      if: always()
+      shell: bash
+      run: |
+        sudo rm -rf /home/ubuntu/tests/
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index a733b9c77..1e8772fac 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -14,7 +14,7 @@ jobs:
   run-platform:
     name: Run AWS full tests
     # run only if the PR is approved by at least 2 reviewers and against the master/main branch or manually triggered
-    if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved' && (github.event.pull_request.base.ref == 'master' || github.event.pull_request.base.ref == 'main') || github.event_name == 'workflow_dispatch'
+    if: github.repository == 'nf-core/rnafusion' && github.event.review.state == 'approved' && (github.event.pull_request.base.ref == 'master' || github.event.pull_request.base.ref == 'main') || github.event_name == 'workflow_dispatch' || github.event_name == 'release'
     runs-on: ubuntu-latest
     steps:
       - name: Set revision variable
@@ -40,7 +40,7 @@ jobs:
             }
           profiles: test_full
 
-      - uses: actions/upload-artifact@v4
+      - uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
         with:
           name: Seqera Platform debug log file
           path: |
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index d3448f1be..5aa6fa3b8 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -25,7 +25,7 @@ jobs:
             }
           profiles: test
 
-      - uses: actions/upload-artifact@v4
+      - uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
         with:
           name: Seqera Platform debug log file
           path: |
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
deleted file mode 100644
index 59b0d7c77..000000000
--- a/.github/workflows/ci.yml
+++ /dev/null
@@ -1,88 +0,0 @@
-name: nf-core CI
-# This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors
-on:
-  push:
-    branches:
-      - dev
-  pull_request:
-  release:
-    types: [published]
-  workflow_dispatch:
-
-env:
-  NXF_ANSI_LOG: false
-  NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
-  NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
-
-concurrency:
-  group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}"
-  cancel-in-progress: true
-
-jobs:
-  test:
-    name: "Run pipeline with test data (${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }})"
-    # Only run on push if this is the nf-core dev branch (merged PRs)
-    if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnafusion') }}"
-    runs-on: ubuntu-latest
-    strategy:
-      matrix:
-        NXF_VER:
-          - "24.04.2"
-          - "latest-everything"
-        profile:
-          - "conda"
-          - "docker"
-          - "singularity"
-        test_name:
-          - "test"
-        isMaster:
-          - ${{ github.base_ref == 'master' }}
-        # Exclude conda and singularity on dev
-        exclude:
-          - isMaster: false
-            profile: "conda"
-          - isMaster: false
-            profile: "singularity"
-    steps:
-      - name: Check out pipeline code
-        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
-        with:
-          fetch-depth: 0
-
-      - name: Set up Nextflow
-        uses: nf-core/setup-nextflow@v2
-        with:
-          version: "${{ matrix.NXF_VER }}"
-
-      - name: Set up Apptainer
-        if: matrix.profile == 'singularity'
-        uses: eWaterCycle/setup-apptainer@main
-
-      - name: Set up Singularity
-        if: matrix.profile == 'singularity'
-        run: |
-          mkdir -p $NXF_SINGULARITY_CACHEDIR
-          mkdir -p $NXF_SINGULARITY_LIBRARYDIR
-
-      - name: Set up Miniconda
-        if: matrix.profile == 'conda'
-        uses: conda-incubator/setup-miniconda@a4260408e20b96e80095f42ff7f1a15b27dd94ca # v3
-        with:
-          miniconda-version: "latest"
-          auto-update-conda: true
-          conda-solver: libmamba
-          channels: conda-forge,bioconda
-
-      - name: Set up Conda
-        if: matrix.profile == 'conda'
-        run: |
-          echo $(realpath $CONDA)/condabin >> $GITHUB_PATH
-          echo $(realpath python) >> $GITHUB_PATH
-
-      - name: Clean up Disk space
-        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
-
-      - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
-        continue-on-error: ${{ matrix.NXF_VER == 'latest-everything' }}
-        run: |
-          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} --outdir ./results
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index 0b6b1f272..ac030fd58 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9
+      - uses: actions/stale@5bef64f19d7facfb25b37b414482c7164d639639 # v9
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index ab06316ea..999bcc382 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -12,14 +12,6 @@ on:
         required: true
         default: "dev"
   pull_request:
-    types:
-      - opened
-      - edited
-      - synchronize
-    branches:
-      - main
-      - master
-  pull_request_target:
     branches:
       - main
       - master
@@ -52,9 +44,9 @@ jobs:
       - name: Disk space cleanup
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
+      - uses: actions/setup-python@a26af69be951a213d495a4c3e4e4022e16d87065 # v5
         with:
-          python-version: "3.12"
+          python-version: "3.13"
           architecture: "x64"
 
       - name: Setup Apptainer
@@ -120,6 +112,7 @@ jobs:
           echo "IMAGE_COUNT_AFTER=$image_count" >> "$GITHUB_OUTPUT"
 
       - name: Compare container image counts
+        id: count_comparison
         run: |
           if [ "${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}" -ne "${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}" ]; then
             initial_count=${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}
@@ -132,3 +125,10 @@ jobs:
           else
             echo "The pipeline can be downloaded successfully!"
           fi
+
+      - name: Upload Nextflow logfile for debugging purposes
+        uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
+        with:
+          name: nextflow_logfile.txt
+          path: .nextflow.log*
+          include-hidden-files: true
diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix_linting.yml
similarity index 96%
rename from .github/workflows/fix-linting.yml
rename to .github/workflows/fix_linting.yml
index 1e4ccb869..7304c0f52 100644
--- a/.github/workflows/fix-linting.yml
+++ b/.github/workflows/fix_linting.yml
@@ -32,9 +32,9 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
       # Install and run pre-commit
-      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
+      - uses: actions/setup-python@a26af69be951a213d495a4c3e4e4022e16d87065 # v5
         with:
-          python-version: "3.12"
+          python-version: "3.13"
 
       - name: Install pre-commit
         run: pip install pre-commit
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index dbd52d5a2..f2d7d1dd7 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -3,9 +3,6 @@ name: nf-core linting
 # It runs the `nf-core pipelines lint` and markdown lint tests to ensure
 # that the code meets the nf-core guidelines.
 on:
-  push:
-    branches:
-      - dev
   pull_request:
   release:
     types: [published]
@@ -17,9 +14,9 @@ jobs:
       - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Set up Python 3.12
-        uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
+        uses: actions/setup-python@a26af69be951a213d495a4c3e4e4022e16d87065 # v5
         with:
-          python-version: "3.12"
+          python-version: "3.13"
 
       - name: Install pre-commit
         run: pip install pre-commit
@@ -36,13 +33,13 @@ jobs:
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
+      - uses: actions/setup-python@a26af69be951a213d495a4c3e4e4022e16d87065 # v5
         with:
-          python-version: "3.12"
+          python-version: "3.13"
           architecture: "x64"
 
       - name: read .nf-core.yml
-        uses: pietrobolcato/action-read-yaml@1.1.0
+        uses: pietrobolcato/action-read-yaml@9f13718d61111b69f30ab4ac683e67a56d254e1d # 1.1.0
         id: read_yml
         with:
           config: ${{ github.workspace }}/.nf-core.yml
@@ -74,7 +71,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@b4b15b8c7c6ac21ea08fcf65892d2ee8f75cf882 # v4
+        uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 95b6b6af8..7e8050fb8 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@20319c5641d495c8a52e688b7dc5fada6c3a9fbc # v8
+        uses: dawidd6/action-download-artifact@4c1e823582f43b179e2cbb49c3eade4e41f992e2 # v10
         with:
           workflow: linting.yml
           workflow_conclusion: completed
@@ -21,7 +21,7 @@ jobs:
         run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2
+        uses: marocchino/sticky-pull-request-comment@52423e01640425a022ef5fd42c6fb5f633a02728 # v2
         with:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
new file mode 100644
index 000000000..f03aea0c0
--- /dev/null
+++ b/.github/workflows/nf-test.yml
@@ -0,0 +1,142 @@
+name: Run nf-test
+on:
+  push:
+    paths-ignore:
+      - "docs/**"
+      - "**/meta.yml"
+      - "**/*.md"
+      - "**/*.png"
+      - "**/*.svg"
+  pull_request:
+    paths-ignore:
+      - "docs/**"
+      - "**/meta.yml"
+      - "**/*.md"
+      - "**/*.png"
+      - "**/*.svg"
+  release:
+    types: [published]
+  workflow_dispatch:
+
+# Cancel if a newer run is started
+concurrency:
+  group: ${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}
+  cancel-in-progress: true
+
+env:
+  GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+  NFT_VER: "0.9.2"
+  NFT_WORKDIR: "~"
+  NXF_ANSI_LOG: false
+  NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
+  NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
+
+jobs:
+  nf-test-changes:
+    name: nf-test-changes
+    runs-on: # use self-hosted runners
+      - runs-on=$-nf-test-changes
+      - runner=4cpu-linux-x64
+    outputs:
+      shard: ${{ steps.set-shards.outputs.shard }}
+      total_shards: ${{ steps.set-shards.outputs.total_shards }}
+    steps:
+      - name: Clean Workspace # Purge the workspace in case it's running on a self-hosted runner
+        run: |
+          ls -la ./
+          rm -rf ./* || true
+          rm -rf ./.??* || true
+          ls -la ./
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
+        with:
+          fetch-depth: 0
+
+      - name: get number of shards
+        id: set-shards
+        uses: ./.github/actions/get-shards
+        env:
+          NFT_VER: ${{ env.NFT_VER }}
+        with:
+          max_shards: 7
+
+      - name: debug
+        run: |
+          echo ${{ steps.set-shards.outputs.shard }}
+          echo ${{ steps.set-shards.outputs.total_shards }}
+
+  nf-test:
+    name: "${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-changes.outputs.total_shards }}"
+    needs: [nf-test-changes]
+    if: ${{ needs.nf-test-changes.outputs.total_shards != '0' }}
+    runs-on: # use self-hosted runners
+      - runs-on=$-nf-test
+      - runner=4cpu-linux-x64
+    strategy:
+      fail-fast: false
+      matrix:
+        shard: ${{ fromJson(needs.nf-test-changes.outputs.shard) }}
+        profile: [conda, docker, singularity]
+        isMain:
+          - ${{ github.base_ref == 'master' || github.base_ref == 'main' }}
+        # Exclude conda and singularity on dev
+        exclude:
+          - isMain: false
+            profile: "conda"
+          - isMain: false
+            profile: "singularity"
+        NXF_VER:
+          - "24.04.2"
+          - "latest-everything"
+    env:
+      NXF_ANSI_LOG: false
+      TOTAL_SHARDS: ${{ needs.nf-test-changes.outputs.total_shards }}
+
+    steps:
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
+        with:
+          fetch-depth: 0
+
+      - name: Run nf-test
+        uses: ./.github/actions/nf-test
+        env:
+          NFT_DIFF: ${{ env.NFT_DIFF }}
+          NFT_DIFF_ARGS: ${{ env.NFT_DIFF_ARGS }}
+          NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
+        with:
+          profile: ${{ matrix.profile }}
+          shard: ${{ matrix.shard }}
+          total_shards: ${{ env.TOTAL_SHARDS }}
+  confirm-pass:
+    needs: [nf-test]
+    if: always()
+    runs-on: # use self-hosted runners
+      - runs-on=$-confirm-pass
+      - runner=2cpu-linux-x64
+    steps:
+      - name: One or more tests failed
+        if: ${{ contains(needs.*.result, 'failure') }}
+        run: exit 1
+
+      - name: One or more tests cancelled
+        if: ${{ contains(needs.*.result, 'cancelled') }}
+        run: exit 1
+
+      - name: All tests ok
+        if: ${{ contains(needs.*.result, 'success') }}
+        run: exit 0
+
+      - name: debug-print
+        if: always()
+        run: |
+          echo "::group::DEBUG: `needs` Contents"
+          echo "DEBUG: toJSON(needs) = ${{ toJSON(needs) }}"
+          echo "DEBUG: toJSON(needs.*.result) = ${{ toJSON(needs.*.result) }}"
+          echo "::endgroup::"
+
+      - name: Clean Workspace # Purge the workspace in case it's running on a self-hosted runner
+        if: always()
+        run: |
+          ls -la ./
+          rm -rf ./* || true
+          rm -rf ./.??* || true
+          ls -la ./
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 76a9e67ed..4abaf4843 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -30,7 +30,7 @@ jobs:
   bsky-post:
     runs-on: ubuntu-latest
     steps:
-      - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0
+      - uses: zentered/bluesky-post-action@4aa83560bb3eac05dbad1e5f221ee339118abdd2 # v0.2.0
         with:
           post: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template-version-comment.yml
similarity index 95%
rename from .github/workflows/template_version_comment.yml
rename to .github/workflows/template-version-comment.yml
index 537529bc1..beb5c77fb 100644
--- a/.github/workflows/template_version_comment.yml
+++ b/.github/workflows/template-version-comment.yml
@@ -14,7 +14,7 @@ jobs:
           ref: ${{ github.event.pull_request.head.sha }}
 
       - name: Read template version from .nf-core.yml
-        uses: nichmor/minimal-read-yaml@v0.0.2
+        uses: nichmor/minimal-read-yaml@1f7205277e25e156e1f63815781db80a6d490b8f # v0.0.2
         id: read_yml
         with:
           config: ${{ github.workspace }}/.nf-core.yml
diff --git a/.nf-core.yml b/.nf-core.yml
index 4431e715a..3eb57693e 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,7 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
-nf_core_version: 3.2.1
+nf_core_version: 3.3.1
 repository_type: pipeline
 template:
   author: Martin Proks, Annick Renevey
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 1dec86502..9d0b248d3 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -4,10 +4,24 @@ repos:
     hooks:
       - id: prettier
         additional_dependencies:
-          - prettier@3.2.5
-
-  - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
-    rev: "3.1.2"
+          - prettier@3.5.0
+  - repo: https://github.com/pre-commit/pre-commit-hooks
+    rev: v5.0.0
     hooks:
-      - id: editorconfig-checker
-        alias: ec
+      - id: trailing-whitespace
+        args: [--markdown-linebreak-ext=md]
+        exclude: |
+          (?x)^(
+              .*ro-crate-metadata.json$|
+              modules/nf-core/.*|
+              subworkflows/nf-core/.*|
+              .*\.snap$
+          )$
+      - id: end-of-file-fixer
+        exclude: |
+          (?x)^(
+              .*ro-crate-metadata.json$|
+              modules/nf-core/.*|
+              subworkflows/nf-core/.*|
+              .*\.snap$
+          )$
diff --git a/.prettierrc.yml b/.prettierrc.yml
index c81f9a766..07dbd8bb9 100644
--- a/.prettierrc.yml
+++ b/.prettierrc.yml
@@ -1 +1,6 @@
 printWidth: 120
+tabWidth: 4
+overrides:
+  - files: "*.{md,yml,yaml,html,css,scss,js,cff}"
+    options:
+      tabWidth: 2
diff --git a/README.md b/README.md
index f40e75a67..1bd640313 100644
--- a/README.md
+++ b/README.md
@@ -9,13 +9,14 @@
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
-[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.04.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
+[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.1)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
 [![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)
 
-[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
 
 ## Introduction
 
@@ -28,7 +29,7 @@
 -->
 
 <!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
-     workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->
+     workflows use the "tube map" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->
 <!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 
 ## Usage
diff --git a/conf/base.config b/conf/base.config
index c0779b554..e8c3525d6 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -15,7 +15,7 @@ process {
     memory = { 6.GB   * task.attempt }
     time   = { 4.h    * task.attempt }
 
-    errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' }
+    errorStrategy = { task.exitStatus in ((130..145) + 104 + 175) ? 'retry' : 'finish' }
     maxRetries    = 1
     maxErrors     = '-1'
 
@@ -59,4 +59,7 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
+    withLabel: process_gpu {
+        ext.use_gpu = { workflow.profile.contains('gpu') }
+    }
 }
diff --git a/nextflow.config b/nextflow.config
index 0322d3a80..c8fe40eab 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -160,16 +160,25 @@ profiles {
             ]
         }
     }
+    gpu {
+        docker.runOptions       = '-u $(id -u):$(id -g) --gpus all'
+        apptainer.runOptions    = '--nv'
+        singularity.runOptions  = '--nv'
+    }
     test      { includeConfig 'conf/test.config'      }
     test_full { includeConfig 'conf/test_full.config' }
 }
 
-// Load nf-core custom profiles from different Institutions
-includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null"
+// Load nf-core custom profiles from different institutions
+
+// If params.custom_config_base is set AND either the NXF_OFFLINE environment variable is not set or params.custom_config_base is a local path, the nfcore_custom.config file from the specified base path is included.
+// Load nf-core/rnafusion custom profiles from different institutions.
+includeConfig params.custom_config_base && (!System.getenv('NXF_OFFLINE') || !params.custom_config_base.startsWith('http')) ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null"
+
 
 // Load nf-core/rnafusion custom profiles from different institutions.
 // TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
-// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/rnafusion.config" : "/dev/null"
+// includeConfig params.custom_config_base && (!System.getenv('NXF_OFFLINE') || !params.custom_config_base.startsWith('http')) ? "${params.custom_config_base}/pipeline/rnafusion.config" : "/dev/null"
 
 // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile
 // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled
@@ -257,7 +266,7 @@ manifest {
 
 // Nextflow plugins
 plugins {
-    id 'nf-schema@2.2.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+    id 'nf-schema@2.3.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 
 validation {
diff --git a/nf-test.config b/nf-test.config
new file mode 100644
index 000000000..889df7601
--- /dev/null
+++ b/nf-test.config
@@ -0,0 +1,24 @@
+config {
+    // location for all nf-test tests
+    testsDir "."
+
+    // nf-test directory including temporary files for each test
+    workDir System.getenv("NFT_WORKDIR") ?: ".nf-test"
+
+    // location of an optional nextflow.config file specific for executing tests
+    configFile "tests/nextflow.config"
+
+    // ignore tests coming from the nf-core/modules repo
+    ignore 'modules/nf-core/**/*', 'subworkflows/nf-core/**/*'
+
+    // run all test with defined profile(s) from the main nextflow.config
+    profile "test"
+
+    // list of filenames or patterns that should be trigger a full test run
+    triggers 'nextflow.config', 'nf-test.config', 'conf/test.config', 'tests/nextflow.config', 'tests/.nftignore'
+
+    // load the necessary plugins
+    plugins {
+        load "nft-utils@0.0.3"
+    }
+}
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index ccf7d6388..21ffe6a16 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,8 +22,8 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
-            "datePublished": "2025-04-30T12:27:54+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "datePublished": "2025-06-03T11:02:37+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.04.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#2c818c4f-fbf7-42fa-9200-2b8dc9844428"
+                    "@id": "#b677eda3-711d-4250-b4fa-fc2ec307db84"
                 }
             ],
             "name": "nf-core/rnafusion"
@@ -137,7 +137,7 @@
                 }
             ],
             "dateCreated": "",
-            "dateModified": "2025-04-30T12:27:54Z",
+            "dateModified": "2025-06-03T11:02:37Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
             "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
             "license": ["MIT"],
@@ -169,11 +169,11 @@
             "version": "!>=24.04.2"
         },
         {
-            "@id": "#2c818c4f-fbf7-42fa-9200-2b8dc9844428",
+            "@id": "#b677eda3-711d-4250-b4fa-fc2ec307db84",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#c6d68ba1-a887-413a-b175-e9947384e9d7"
+                    "@id": "#d7d8d23f-bc32-4572-bb73-a559c46d3773"
                 }
             ],
             "mainEntity": {
@@ -182,10 +182,10 @@
             "name": "Test suite for nf-core/rnafusion"
         },
         {
-            "@id": "#c6d68ba1-a887-413a-b175-e9947384e9d7",
+            "@id": "#d7d8d23f-bc32-4572-bb73-a559c46d3773",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/rnafusion",
-            "resource": "repos/nf-core/rnafusion/actions/workflows/ci.yml",
+            "resource": "repos/nf-core/rnafusion/actions/workflows/nf-test.yml",
             "runsOn": {
                 "@id": "https://w3id.org/ro/terms/test#GithubService"
             },
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 9ec640a61..0403e6035 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -261,4 +261,3 @@ def methodsDescriptionText(mqc_methods_yaml) {
 
     return description_html.toString()
 }
-
diff --git a/tests/.nftignore b/tests/.nftignore
new file mode 100644
index 000000000..c10bc1f11
--- /dev/null
+++ b/tests/.nftignore
@@ -0,0 +1,10 @@
+.DS_Store
+multiqc/multiqc_data/fastqc_top_overrepresented_sequences_table.txt
+multiqc/multiqc_data/multiqc.log
+multiqc/multiqc_data/multiqc_data.json
+multiqc/multiqc_data/multiqc_sources.txt
+multiqc/multiqc_data/multiqc_software_versions.txt
+multiqc/multiqc_plots/{svg,pdf,png}/*.{svg,pdf,png}
+multiqc/multiqc_report.html
+fastqc/*_fastqc.{html,zip}
+pipeline_info/*.{html,json,txt,yml}
diff --git a/tests/default.nf.test b/tests/default.nf.test
new file mode 100644
index 000000000..0d7279dc0
--- /dev/null
+++ b/tests/default.nf.test
@@ -0,0 +1,35 @@
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    tag "pipeline"
+
+    test("-profile test") {
+
+        when {
+            params {
+                outdir = "$outputDir"
+            }
+        }
+
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we test pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    stable_path
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/tests/nextflow.config b/tests/nextflow.config
new file mode 100644
index 000000000..c00c384c5
--- /dev/null
+++ b/tests/nextflow.config
@@ -0,0 +1,12 @@
+/*
+========================================================================================
+    Nextflow config file for running nf-test tests
+========================================================================================
+*/
+
+// TODO nf-core: Specify any additional parameters here
+// Or any resources requirements
+params.modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
+params.pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion'
+
+aws.client.anonymous = true // fixes S3 access issues on self-hosted runners

From 77b107332be9ab9aa7871486bc052537aea26b91 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 3 Jun 2025 13:50:19 +0200
Subject: [PATCH 526/887] update memory

---
 conf/modules.config | 1 +
 1 file changed, 1 insertion(+)

diff --git a/conf/modules.config b/conf/modules.config
index 17e798cf7..f4316f431 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -104,6 +104,7 @@ process {
 
     withName: 'FUSIONCATCHER_FUSIONCATCHER' {
         ext.args = {[
+            "--Xmx 18g",
             "--limitSjdbInsertNsj ${params.fusioncatcher_limitSjdbInsertNsj}",
             "--skip-blat",
             meta.single_end ? "--single-end" : "",

From 8fc4ad97f0a97dd4d18996ed2192cca566d40ee9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 11:50:15 +0200
Subject: [PATCH 527/887] change trim condition

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index ee970fe9f..5d2eaa02b 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -255,7 +255,7 @@ workflow RNAFUSION {
         def ch_fusioncatcher_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
             fusions_created = true
-            fusioncatcher_trimming = { params.tools.contains('fusioncatcher_trim') ?: false }
+            fusioncatcher_trimming = params.tools.contains('fusioncatcher_trim') ?: false
             FUSIONCATCHER_WORKFLOW (
                 ch_reads,
                 fusioncatcher_trimming,

From 25b700b61564650488fe026211c0b25a97a1cc80 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 12:58:40 +0200
Subject: [PATCH 528/887] link memory usage with task.memory

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index f4316f431..58cafcbd9 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -104,7 +104,7 @@ process {
 
     withName: 'FUSIONCATCHER_FUSIONCATCHER' {
         ext.args = {[
-            "--Xmx 18g",
+            "--Xmx ${task.memory.toGiga()-2}g",
             "--limitSjdbInsertNsj ${params.fusioncatcher_limitSjdbInsertNsj}",
             "--skip-blat",
             meta.single_end ? "--single-end" : "",

From c8a1471ef2ccd7f76706efb0a45abb445077cd6f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 13:15:00 +0200
Subject: [PATCH 529/887] update changelog

---
 CHANGELOG.md | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 94c5a7d6b..ef6b5a3a5 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -45,6 +45,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added `--star_limit_bam_sort_ram` to set the maximum memory for sorting the BAM files in STAR. [#668](https://github.com/nf-core/rnafusion/pull/668)
 - Update STAR-Fusion to 1.15.0 and update nf-core modules [#664](https://github.com/nf-core/rnafusion/pull/664)
 - Update fusionreport 4.0.0 that replaces the score of a fusion with a Fusion Indication Index [#667](https://github.com/nf-core/rnafusion/pull/667)
+- Added a extra trimming step for fusioncatcher in case a different read-length is wished for this tool only [#674](https://github.com/nf-core/rnafusion/pull/674)
 
 ### Changed
 
@@ -118,6 +119,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | `--stringtie`         | `--tools stringtie`             |
 | `--all`               | `--tools all`                   |
 |                       | `--fusioncatcher_download_link` |
+|                       | `--tools fusioncatcher_trim`    |
+|                       | `--trim_tail_fusioncatcher`     |
 
 ## v3.0.2 - [2024-04-10]
 

From ee255df1160c05621ade913c22bec3834854e287 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 13:15:12 +0200
Subject: [PATCH 530/887] update contributors

---
 README.md | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 809de85f1..c3051ad89 100644
--- a/README.md
+++ b/README.md
@@ -114,11 +114,13 @@ For more details about the output files and reports, please refer to the
 nf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))
 
 We thank the following people for their help in the development of this pipeline:
-
+-
 - [Phil Ewels](https://github.com/ewels)
 - [Rickard Hammarén](https://github.com/Hammarn)
 - [Alexander Peltzer](https://github.com/apeltzer)
 - [Praveen Raj](https://github.com/praveenraj2018)
+- [Anabella Trigila](https://github.com/atrigila)
+- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)
 
 ## Contributions and Support
 

From 867f97e85d436ce6cef1a63ec656ceeef24ce7ec Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 13:15:27 +0200
Subject: [PATCH 531/887] reshuffle memory allocation

---
 conf/modules.config                                 | 1 -
 modules/nf-core/fusioncatcher/fusioncatcher/main.nf | 2 +-
 2 files changed, 1 insertion(+), 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 58cafcbd9..17e798cf7 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -104,7 +104,6 @@ process {
 
     withName: 'FUSIONCATCHER_FUSIONCATCHER' {
         ext.args = {[
-            "--Xmx ${task.memory.toGiga()-2}g",
             "--limitSjdbInsertNsj ${params.fusioncatcher_limitSjdbInsertNsj}",
             "--skip-blat",
             meta.single_end ? "--single-end" : "",
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
index 5c2a8f38d..0b9a4ad13 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
@@ -37,7 +37,7 @@ process FUSIONCATCHER_FUSIONCATCHER {
         --output=. \\
         --data=${reference} \\
         --threads=${task.cpus} \\
-        --Xmx=${avail_mem} \\
+        --Xmx=${avail_mem.toGiga()}g \\
         ${args}
 
     mv final-list_candidate-fusion-genes.txt ${prefix}.fusion-genes.txt

From e0d9840fef088e6fcde9a0d6d8c6ca1b1de8be1e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 13:27:42 +0200
Subject: [PATCH 532/887] reshuffle memory allocation

---
 modules/nf-core/fusioncatcher/fusioncatcher/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
index 0b9a4ad13..bca7ccd98 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
@@ -37,7 +37,7 @@ process FUSIONCATCHER_FUSIONCATCHER {
         --output=. \\
         --data=${reference} \\
         --threads=${task.cpus} \\
-        --Xmx=${avail_mem.toGiga()}g \\
+        --Xmx${avail_mem}M \\
         ${args}
 
     mv final-list_candidate-fusion-genes.txt ${prefix}.fusion-genes.txt

From 2f0bc730bd5dd63e76fc34413dd153fa8e89b197 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 13:35:03 +0200
Subject: [PATCH 533/887] reshuffle memory allocation

---
 modules/nf-core/fusioncatcher/fusioncatcher/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
index bca7ccd98..3abd90d70 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
@@ -37,7 +37,7 @@ process FUSIONCATCHER_FUSIONCATCHER {
         --output=. \\
         --data=${reference} \\
         --threads=${task.cpus} \\
-        --Xmx${avail_mem}M \\
+        --Xmx=${avail_mem}M \\
         ${args}
 
     mv final-list_candidate-fusion-genes.txt ${prefix}.fusion-genes.txt

From 8b3dd01a1a2b858b07da66b2850f7f59196cf752 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 14:51:59 +0200
Subject: [PATCH 534/887] update module

---
 modules.json                                  |  2 +-
 .../fusioncatcher/fusioncatcher/meta.yml      | 62 +++++++++----------
 2 files changed, 32 insertions(+), 32 deletions(-)

diff --git a/modules.json b/modules.json
index 844a7b83d..b7ce82d85 100644
--- a/modules.json
+++ b/modules.json
@@ -42,7 +42,7 @@
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
-                        "git_sha": "2a227f75f33415f74a884ef8b33555b151e33de9",
+                        "git_sha": "1037205706929921360f57e8042eae6819825e31",
                         "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
index ff6647d65..bfd7848ef 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
@@ -37,40 +37,40 @@ input:
 
 output:
   - fusions:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. `[ id:'sample1', single_end:false ]`
-      - "*.fusion-genes.txt":
-          type: file
-          description: The detected fusions in the input files
-          pattern: "*.fusion-genes.txt"
+    - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - "*.fusion-genes.txt":
+        type: file
+        description: The detected fusions in the input files
+        pattern: "*.fusion-genes.txt"
   - summary:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. `[ id:'sample1', single_end:false ]`
-      - "*.summary.txt":
-          type: file
-          description: The generated summary
-          pattern: "*.summary.txt"
+    - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - "*.summary.txt":
+        type: file
+        description: The generated summary
+        pattern: "*.summary.txt"
   - log:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. `[ id:'sample1', single_end:false ]`
-      - "*.log":
-          type: file
-          description: The Fusioncatcher log
-          pattern: "*.log"
+    - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - "*.log":
+        type: file
+        description: The Fusioncatcher log
+        pattern: "*.log"
   - versions:
-      - "versions.yml":
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+    - "versions.yml":
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
 
 authors:
   - "@nvnieuwk"

From c0c95ddcfcbec95ef3113e9b3cec783d5e4152f4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 4 Jun 2025 14:55:05 +0200
Subject: [PATCH 535/887] prettier

---
 README.md                                     |  4 +-
 .../fusioncatcher/fusioncatcher/meta.yml      | 62 +++++++++----------
 2 files changed, 33 insertions(+), 33 deletions(-)

diff --git a/README.md b/README.md
index c3051ad89..92dd1aac9 100644
--- a/README.md
+++ b/README.md
@@ -113,8 +113,8 @@ For more details about the output files and reports, please refer to the
 
 nf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))
 
-We thank the following people for their help in the development of this pipeline:
--
+## We thank the following people for their help in the development of this pipeline:
+
 - [Phil Ewels](https://github.com/ewels)
 - [Rickard Hammarén](https://github.com/Hammarn)
 - [Alexander Peltzer](https://github.com/apeltzer)
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
index bfd7848ef..ff6647d65 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
@@ -37,40 +37,40 @@ input:
 
 output:
   - fusions:
-    - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - "*.fusion-genes.txt":
-        type: file
-        description: The detected fusions in the input files
-        pattern: "*.fusion-genes.txt"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.fusion-genes.txt":
+          type: file
+          description: The detected fusions in the input files
+          pattern: "*.fusion-genes.txt"
   - summary:
-    - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - "*.summary.txt":
-        type: file
-        description: The generated summary
-        pattern: "*.summary.txt"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.summary.txt":
+          type: file
+          description: The generated summary
+          pattern: "*.summary.txt"
   - log:
-    - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. `[ id:'sample1', single_end:false ]`
-    - "*.log":
-        type: file
-        description: The Fusioncatcher log
-        pattern: "*.log"
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - "*.log":
+          type: file
+          description: The Fusioncatcher log
+          pattern: "*.log"
   - versions:
-    - "versions.yml":
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+      - "versions.yml":
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 
 authors:
   - "@nvnieuwk"

From 732ebddeab95c643e692632992b70c91cc4b62f4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 5 Jun 2025 11:26:13 +0200
Subject: [PATCH 536/887] update documentation

---
 docs/output.md | 18 +++++++++++++++++-
 docs/usage.md  |  2 ++
 2 files changed, 19 insertions(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index 2395eb624..de9ab367d 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -133,7 +133,7 @@ If multiple libraries or runs have been provided for the same sample in the inpu
 
 ### Fastp
 
-If `--trim_fastp` is selected, [fastp](https://github.com/OpenGene/fastp) will filter low quality reads as well as bases at the 5' and 3' ends, trim adapters (automatically detected, but input with parameter `--adapter_fasta` is possible). 3' trimming is also possible via parameter `--trim_tail`.
+If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will filter low quality reads as well as bases at the 5' and 3' ends, trim adapters (automatically detected, but input with parameter `--adapter_fasta` is possible). 3' trimming is also possible via parameter `--trim_tail`.
 
 <details markdown="1">
 <summary>Output files</summary>
@@ -147,6 +147,22 @@ If `--trim_fastp` is selected, [fastp](https://github.com/OpenGene/fastp) will f
 
 </details>
 
+### Fastp for fusioncatcher
+
+If `--tools fusioncatcher_trim` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This to potentially shorten the reads fed into fusioncatcher further with `--trim_tail_fusioncatcher` if the reads are above 100 bp.
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `fastp/`
+  - `<sample>_trimmed_for_fusioncatcher_1.fastp.fastq.gz`
+  - `<sample>_trimmed_for_fusioncatcher_2.fastp.fastq.gz`
+  - `<sample>_trimmed_for_fusioncatcher.fastp.html`
+  - `<sample>_trimmed_for_fusioncatcher.fastp.json`
+  - `<sample>_trimmed_for_fusioncatcher.fastp.log`
+
+</details>
+
 ### FastQC
 
 <details markdown="1">
diff --git a/docs/usage.md b/docs/usage.md
index ae5fe8f58..300d9e320 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -268,6 +268,8 @@ nextflow run nf-core/rnafusion \
 --adapter_fastq <PATH/TO/ADAPTER/FASTQ> (optional)
 ```
 
+The additional fusioncatcher_trim flag in `--tools` will toggle an additional `fastp` process, especially useful is reads are above 100 bp, which is not handled well by fusioncatcher. The parameter `--trim_tail_fusioncatcher` will trim the extra read length from the tails.
+
 #### Filter fusions detected by 2 or more tools
 
 ```bash

From f0c010865238be32c262f75bfe8f2f82cf60a377 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 6 Jun 2025 09:26:49 +0200
Subject: [PATCH 537/887] Update docs/output.md

Co-authored-by: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
---
 docs/output.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index de9ab367d..ae231e938 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -149,7 +149,7 @@ If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will f
 
 ### Fastp for fusioncatcher
 
-If `--tools fusioncatcher_trim` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This to potentially shorten the reads fed into fusioncatcher further with `--trim_tail_fusioncatcher` if the reads are above 100 bp.
+If `--tools fusioncatcher_trim` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This is to potentially shorten the reads fed into `fusioncatcher` further with `--trim_tail_fusioncatcher` if the reads are above 100 bp.
 
 <details markdown="1">
 <summary>Output files</summary>

From b6e4c6b764f2933451457e168f0ac9a18b636bcd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 13 Jun 2025 16:37:47 +0200
Subject: [PATCH 538/887] use trim_tail default as 0

---
 conf/modules.config                           |    4 +-
 nextflow.config                               |    4 +-
 .../fusioncatcher_workflow/tests/main.nf.test |   53 +
 .../tests/main.nf.test.snap                   |   15 +
 .../fusionreport_workflow/tests/main.nf.test  |   17 +-
 tests/test_stub.nf.test                       |   12 +-
 tests/test_stub.nf.test.snap                  | 1918 +----------------
 7 files changed, 150 insertions(+), 1873 deletions(-)
 create mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
 create mode 100644 subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap

diff --git a/conf/modules.config b/conf/modules.config
index 17e798cf7..f4f84cc65 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -65,7 +65,7 @@ process {
     }
 
     withName: 'FASTP' {
-        ext.args = { params.trim_tail ? "--trim_tail1 ${params.trim_tail} --trim_tail2 ${params.trim_tail} " : '' }
+        ext.args = { params.trim_tail > 0 ? "--trim_tail1 ${params.trim_tail} --trim_tail2 ${params.trim_tail} " : '' }
         publishDir = [
             path: { "${params.outdir}/fastp" },
             mode: params.publish_dir_mode,
@@ -74,7 +74,7 @@ process {
     }
 
     withName: 'FASTP_FOR_FUSIONCATCHER' {
-        ext.args = { params.trim_tail_fusioncatcher ? "--trim_tail1 ${params.trim_tail_fusioncatcher} --trim_tail2 ${params.trim_tail_fusioncatcher} " : '' }
+        ext.args = { params.trim_tail_fusioncatcher > 0 ? "--trim_tail1 ${params.trim_tail_fusioncatcher} --trim_tail2 ${params.trim_tail_fusioncatcher} " : '' }
         ext.prefix = { "${meta.id}_trimmed_for_fusioncatcher" }
         publishDir = [
             path: { "${params.outdir}/fastp_for_fusioncatcher" },
diff --git a/nextflow.config b/nextflow.config
index 1c22ae581..9f5536013 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -39,8 +39,8 @@ params {
     tools_cutoff               = 1
 
     // Trimming
-    trim_tail                  = null
-    trim_tail_fusioncatcher    = null
+    trim_tail                  = 0
+    trim_tail_fusioncatcher    = 0
     adapter_fasta              = []
 
     // Compression
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
new file mode 100644
index 000000000..05d691889
--- /dev/null
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -0,0 +1,53 @@
+nextflow_workflow {
+
+    name "Test Subworkflow FUSIONCATCHER_WORKFLOW"
+    script "../main.nf"
+    workflow "FUSIONCATCHER_WORKFLOW"
+
+    tag "subworkflow"
+    tag "fusioncatcher_workflow"
+    tag "fusioncatcher"
+
+    test("FUSIONCATCHER_WORKFLOW - Stub") {
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                // Input channels
+                input[0] = Channel.of(
+                    [ [ id:'test_sample' ],
+                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz"),
+                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_2.fq.gz") ]
+                )
+
+                input[1] = true
+
+                input[2] = []
+
+                input[3] = [
+                    [ id:'fusioncatcher_ref' ],
+                    []
+                ]
+
+                input[4] = []
+
+                """
+            }
+            params {
+                tools                   = 'fusioncatcher,fusioncatcher_trim'
+                trim_tail_fusioncatcher = 50
+                outdir                  = "$outputDir"
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(
+                    workflow.out.versions
+                ).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..2e9553a99
--- /dev/null
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -0,0 +1,15 @@
+{
+    "FUSIONCATCHER_WORKFLOW - Stub": {
+        "content": [
+            [
+                "versions.yml:md5,24a191a82bb19899f88f6493ad4f1b8c",
+                "versions.yml:md5,aeee6dd3b4dcf4cb11af80dfa0a7bf72"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
+        },
+        "timestamp": "2025-06-13T16:36:48.833043"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
index 61aaf023d..18a6c23e4 100644
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
@@ -11,10 +11,19 @@ nextflow_workflow {
     test("FUSIONREPORT_WORKFLOW - Full Test") {
 
         setup {
-            run("FUSIONREPORT_DOWNLOAD") {
-                script "../../../../modules/local/fusionreport/download/main.nf"
+            run("FUSIONCATCHER_FUSIONCATCHER") {
+                script "../../../../modules/nf-core/fusioncatcher/detect/main.nf"
                 process {
                     """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
+                ]
+                input[1] = [
+                    [ id:'reference'],
+                    []
+                ]
                     """
                 }
             }
@@ -50,12 +59,12 @@ nextflow_workflow {
             }
             params {
                 fusioninspector_only = false
-                tools_cutoff = 1
+                tools_cutoff    = 1
                 arriba          = true
                 starfusion      = true
                 fusioncatcher   = true
                 no_cosmic       = true
-                outdir               = "$outputDir"
+                outdir          = "$outputDir"
             }
         }
 
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 562908322..a76004bf9 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -109,7 +109,6 @@ nextflow_pipeline {
             params {
                 outdir           = "$outputDir"
                 tools            = "fusioncatcher"
-                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
             }
         }
 
@@ -135,6 +134,17 @@ nextflow_pipeline {
         }
     }
 
+    test("stub test fusioncatcher") {
+
+            when {
+                params {
+                    outdir           = "$outputDir"
+                    tools            = "fusioncatcher,fusioncatcher_trim"
+                    trim_tail_fusioncatcher = 50
+                }
+            }
+        }
+
     test("stub test starfusion") {
 
         when {
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 824773b56..fea7aa0b1 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,357 +1,31 @@
 {
     "stub test starfusion": {
         "content": [
-            16,
-            {
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "SAMTOOLS_INDEX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
-                "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
-                },
-                "STAR_ALIGN": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            0,
+            null,
             [
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
-                "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
-                "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
-                "star",
-                "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai",
-                "star/test.Aligned.unsort.out.bam",
-                "star/test.Chimeric.out.junction",
-                "star/test.Log.final.out",
-                "star/test.Log.out",
-                "star/test.Log.progress.out",
-                "star/test.ReadsPerGene.out.tab",
-                "star/test.SJ.out.tab",
-                "star/test.Signal.UniqueMultiple.str1.out.bg",
-                "star/test.Signal.UniqueMultiple.str1.out.wig",
-                "star/test.out.sam",
-                "star/test.sortedByCoord.out.bam",
-                "star/test.tab",
-                "star/test.toTranscriptome.out.bam",
-                "star/test.unmapped_1.fastq.gz",
-                "star/test.unmapped_2.fastq.gz",
-                "star/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv"
+                "pipeline_info"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T14:03:38.544810125"
+        "timestamp": "2025-06-06T09:52:16.204798"
     },
     "stub test stringtie": {
         "content": [
-            16,
-            {
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "SAMTOOLS_INDEX": {
-                    "samtools": 1.21
-                },
-                "STAR_ALIGN": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.3"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            0,
+            null,
             [
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "gatk4",
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+                "pipeline_info"
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         "meta": {
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-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
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         },
-        "timestamp": "2025-05-07T14:04:11.405954818"
+        "timestamp": "2025-06-06T09:52:44.54581"
     },
     "stub test arriba": {
         "content": [
@@ -496,1584 +170,100 @@
     },
     "stub test fusioninspector": {
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
-                "salmon",
-                "salmon/test",
-                "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
-                "star",
-                "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai",
-                "star/test.Aligned.unsort.out.bam",
-                "star/test.Chimeric.out.junction",
-                "star/test.Log.final.out",
-                "star/test.Log.out",
-                "star/test.Log.progress.out",
-                "star/test.ReadsPerGene.out.tab",
-                "star/test.SJ.out.tab",
-                "star/test.Signal.UniqueMultiple.str1.out.bg",
-                "star/test.Signal.UniqueMultiple.str1.out.wig",
-                "star/test.out.sam",
-                "star/test.sortedByCoord.out.bam",
-                "star/test.tab",
-                "star/test.toTranscriptome.out.bam",
-                "star/test.unmapped_1.fastq.gz",
-                "star/test.unmapped_2.fastq.gz",
-                "star/testXd.out.bam",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv",
-                "stringtie",
-                "stringtie/[:]",
-                "stringtie/[:]/stringtie.merged.gtf",
-                "stringtie/test.ballgown",
-                "stringtie/test.coverage.gtf",
-                "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
+                "pipeline_info"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T14:01:35.330928392"
+        "timestamp": "2025-06-06T09:21:44.763989"
     }
-}
+}
\ No newline at end of file

From f147826a70295a4eddf89bbac0c77ccda9a92301 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 Jun 2025 11:27:43 +0200
Subject: [PATCH 539/887] update container

---
 modules/local/starfusion/build/main.nf  |   4 ++--
 modules/local/starfusion/detect/main.nf |   4 ++--
 output.png                              | Bin 0 -> 348646 bytes
 output_4.png                            | Bin 0 -> 284152 bytes
 output_5.png                            | Bin 0 -> 240837 bytes
 5 files changed, 4 insertions(+), 4 deletions(-)
 create mode 100644 output.png
 create mode 100644 output_4.png
 create mode 100644 output_5.png

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index daf296f33..469756b82 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -4,8 +4,8 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
+        'docker://trinityctat/starfusion:1.15.0' :
+        'trinityctat/starfusion:1.15.0'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 0bf271541..5a5437f90 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,8 +4,8 @@ process STARFUSION {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b0/b0a58b9d30f9c72b22135f85746e10596d568c40a7d9634b13e0a0749cacd21b/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:c2bc5374f142ac93'}"
+        'docker://trinityctat/starfusion:1.15.0' :
+        'trinityctat/starfusion:1.15.0'}"
 
     input:
     tuple val(meta), path(reads), path(junction)
diff --git a/output.png b/output.png
new file mode 100644
index 0000000000000000000000000000000000000000..5f5ccab0c2732a7f3e13dc1fd823bf5f2f001b0e
GIT binary patch
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zfoy6KATIKrB6MC9pb%>glw2He6eI7_B0FTiu(R8gPUDHZy0C0{-;_H+br2of19Yjs
zqZ>>Nu;et5KCmvb5a~|nK;}%0vKVcz(GHpHq7h0tEOV)?W7sg%f&84XqX}+r7B)G;
z#V1BVLX5+urNG=<-e%KverzwfHWI)vaU>(TmIuda)%4ug_d$Xb^pZ}8>Wh8p#Npy7
z_n^>vfxQ+JjimFC7AFS>PxetLCJ04-2)(6lIoUI4BC$m{_c4tV&MeVDvO{PKS)b5e
zvl%hRXP^=6CF1r07)iK&fMh15m9{7RNx>|m>qsI%h*nN^z?bwWnGw{@{>ZS<(2wZ3
znePttKb9K=YK<Z8*AM{T<FSVW7*gQ(DnES0D@VilnIvXjJcr5}Vs`;bl5A+~vBRbJ
zaAlFj<o?Kc_;K10vZr7mOT&@_;hDILA!&MGFb{bWx92Eqe&i7>nFso(+`XL|@UAQs
znORR0vOP&EjNAv4f>{`pLAh&HHvuHJplaH6_T2=$;zE#p!!#??>xM)F3#;`e(m3_f
zj=?2;gxx8E2!rY#E?UyE0u4RXO?Ck<!ptjYNZty<BO*b|u5>C~q>gDi3PGQ20O#9v
z8<VzWSdmX+L1l?2M!;ZvgFRjl{p*tq3@{bosW=|ZFkxpvauH+4dYT*)lZF$F+*UX(
zF&-gy+qY0-y=DgqsgtzJB0?kdw^$6i26bb|%;O1m5=x#nNQs@XkQ#ywD3_RGM<&qq
zg@U`M4FCj^8Q$<2+~XHZWd9!NvsfGsLnuhaUHo;B=mfl$z<-h_h=m@awLMR(t3QH2
z&<yiQO8LT6<}Fdkw0o2mLAv|d(G_`K6{aX%ECpnphZ1W;whV)A6R-$|-M3ho#zE=+
esQTXuIBC2^GdfJ%ne<uY=oG~x2Q%bNef|O1{)}A!

literal 0
HcmV?d00001


From 82a847eac6261784714465bd01ed3e4e9f029c94 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 Jun 2025 11:49:41 +0200
Subject: [PATCH 540/887] update containers

---
 modules/local/starfusion/build/main.nf  | 2 +-
 modules/local/starfusion/detect/main.nf | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 469756b82..7bc99fe30 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -5,7 +5,7 @@ process STARFUSION_BUILD {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'docker://trinityctat/starfusion:1.15.0' :
-        'trinityctat/starfusion:1.15.0'}"
+        'docker.io/trinityctat/starfusion:1.15.0'}"
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 5a5437f90..9fe72323b 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -5,7 +5,7 @@ process STARFUSION {
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'docker://trinityctat/starfusion:1.15.0' :
-        'trinityctat/starfusion:1.15.0'}"
+        'docker.io/trinityctat/starfusion:1.15.0'}"
 
     input:
     tuple val(meta), path(reads), path(junction)

From fb3a03aced541a3835737e104d07a48d08ca1608 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 Jun 2025 12:48:41 +0200
Subject: [PATCH 541/887] update binPath

---
 modules/local/starfusion/build/main.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 7bc99fe30..7212c915e 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -20,6 +20,7 @@ process STARFUSION_BUILD {
     path "versions.yml"   , emit: versions
 
     script:
+    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
     def args = task.ext.args ?: ''
     """
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${pfam_version}/Pfam-A.hmm.gz --no-check-certificate
@@ -32,7 +33,7 @@ process STARFUSION_BUILD {
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
 
 
-    prep_genome_lib.pl \\
+    $binPath \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --dfam_db ${dfam_species}_dfam.hmm \\

From 04c6f75bcb01d8b9c8808807000a3c300c3f7101 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 16 Jun 2025 13:49:59 +0200
Subject: [PATCH 542/887] update test

---
 nextflow_schema.json         |    4 +-
 tests/test_stub.nf.test      |   37 +-
 tests/test_stub.nf.test.snap | 2016 ++++++++++++++++++++++++++++++++--
 3 files changed, 1986 insertions(+), 71 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 56f55d755..239e57a89 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -263,13 +263,13 @@
                 "trim_tail": {
                     "type": "integer",
                     "description": "The amount of bases to trim at the tail of each read, none will be trimmed by default",
-                    "minimum": 1,
+                    "minimum": 0,
                     "fa_icon": "fas fa-cut"
                 },
                 "trim_tail_fusioncatcher": {
                     "type": "integer",
                     "description": "The amount of bases to trim at the tail of each read for fusioncatcher, none will be trimmed by default",
-                    "minimum": 1,
+                    "minimum": 0,
                     "fa_icon": "fas fa-cut"
                 },
                 "adapter_fasta": {
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index a76004bf9..b329ed863 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -109,6 +109,7 @@ nextflow_pipeline {
             params {
                 outdir           = "$outputDir"
                 tools            = "fusioncatcher"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
             }
         }
 
@@ -134,16 +135,38 @@ nextflow_pipeline {
         }
     }
 
-    test("stub test fusioncatcher") {
+    test("stub test fusioncatcher trim") {
+
+        when {
+            params {
+                outdir           = "$outputDir"
+                tools            = "fusioncatcher,fusioncatcher_trim"
+                trim_tail_fusioncatcher = 50
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
 
-            when {
-                params {
-                    outdir           = "$outputDir"
-                    tools            = "fusioncatcher,fusioncatcher_trim"
-                    trim_tail_fusioncatcher = 50
-                }
             }
         }
+        then {
+            // stable_name: All files + folders in ${params.outdir}/ with a stable name
+            // Ignore files with timestamps in their names
+            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+            // stable_path: All files in ${params.outdir}/ with stable content
+            // def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') //TODO uncomment once -stub is removed
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    // Number of successful tasks
+                    workflow.trace.succeeded().size(),
+                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
+                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
+                    // All stable path name, with a relative path
+                    stable_name,
+                    // All files with stable contents
+                    // stable_path // TODO uncomment once -stub is removed
+                ).match() }
+            )
+        }
+    }
 
     test("stub test starfusion") {
 
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index fea7aa0b1..81d69dd73 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,31 +1,357 @@
 {
     "stub test starfusion": {
         "content": [
-            0,
-            null,
+            16,
+            {
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.15.0"
+                },
+                "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
+                    "STAR-Fusion": "1.15.0"
+                },
+                "STAR_ALIGN": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
-                "pipeline_info"
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-06T09:52:16.204798"
+        "timestamp": "2025-06-16T09:22:13.085521"
     },
     "stub test stringtie": {
         "content": [
-            0,
-            null,
+            16,
+            {
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
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-        "timestamp": "2025-06-06T09:52:44.54581"
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@@ -162,108 +488,1674 @@
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+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-06T09:21:44.763989"
+        "timestamp": "2025-06-16T12:46:03.993646"
     }
 }
\ No newline at end of file

From c058f47393d308b838a83e9a2fb05ba89e16bb9f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 09:48:09 +0200
Subject: [PATCH 543/887] publish fusioninspector

---
 conf/modules.config | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 1fb9675b4..cf8bd8bb7 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -106,6 +106,11 @@ process {
     withName: 'FUSIONINSPECTOR' {
         ext.args = { params.fusioninspector_limitSjdbInsertNsj != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
         ext.args2 = '--annotate --examine_coding_effect'
+        publishDir = [
+            path: { "${params.outdir}/fusioninspector/${meta.id}" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
     }
 
     withName: 'FUSIONREPORT' {

From 1d9be85a9f1ebb675b69affe921a0434883f0363 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 09:48:42 +0200
Subject: [PATCH 544/887] update starfusion containers

---
 modules/local/starfusion/build/environment.yml  | 2 +-
 modules/local/starfusion/build/main.nf          | 5 +++--
 modules/local/starfusion/detect/environment.yml | 4 ++--
 modules/local/starfusion/detect/main.nf         | 4 ++--
 4 files changed, 8 insertions(+), 7 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 8ad0c17bf..67f00c3c0 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -4,5 +4,5 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.29
+  - bioconda::minimap2=2.30
   - bioconda::star-fusion=1.15.0
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 7212c915e..2b65f810f 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -4,8 +4,9 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'docker://trinityctat/starfusion:1.15.0' :
-        'docker.io/trinityctat/starfusion:1.15.0'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/47/47bad9870d9b98e40cd0210edd20fcc018f55098a49b7c8d00aa7e90f88c5e5c/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:10b5c8c209d8edaa'}"
+
 
     input:
     tuple val(meta), path(fasta)
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index ef7f93160..67f00c3c0 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -4,5 +4,5 @@ channels:
 dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
-  - bioconda::minimap2=2.28
-  - bioconda::star-fusion=1.14.0
+  - bioconda::minimap2=2.30
+  - bioconda::star-fusion=1.15.0
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 9fe72323b..aee6fe12e 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -4,8 +4,8 @@ process STARFUSION {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'docker://trinityctat/starfusion:1.15.0' :
-        'docker.io/trinityctat/starfusion:1.15.0'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/47/47bad9870d9b98e40cd0210edd20fcc018f55098a49b7c8d00aa7e90f88c5e5c/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:10b5c8c209d8edaa'}"
 
     input:
     tuple val(meta), path(reads), path(junction)

From 77f52870f61995e381d83f6da489c189f8415e38 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 10:56:15 +0200
Subject: [PATCH 545/887] change bin path

---
 modules/local/starfusion/build/main.nf | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 2b65f810f..98bf43ce3 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -21,7 +21,6 @@ process STARFUSION_BUILD {
     path "versions.yml"   , emit: versions
 
     script:
-    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
     def args = task.ext.args ?: ''
     """
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${pfam_version}/Pfam-A.hmm.gz --no-check-certificate
@@ -34,7 +33,7 @@ process STARFUSION_BUILD {
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
 
 
-    $binPath \\
+    prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --dfam_db ${dfam_species}_dfam.hmm \\

From 948bb58117ddb484837f2eb9d692ec3dd0067eea Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 11:01:05 +0200
Subject: [PATCH 546/887] remove output

---
 output.png   | Bin 348646 -> 0 bytes
 output_4.png | Bin 284152 -> 0 bytes
 output_5.png | Bin 240837 -> 0 bytes
 3 files changed, 0 insertions(+), 0 deletions(-)
 delete mode 100644 output.png
 delete mode 100644 output_4.png
 delete mode 100644 output_5.png

diff --git a/output.png b/output.png
deleted file mode 100644
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From eb34f1a4c642378827154217fc25a2f96fd39e4f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 11:18:47 +0200
Subject: [PATCH 547/887] remove fusioncatcher_trim, just use
 trim_tail_fusioncatcher

---
 CHANGELOG.md                                  |   1 -
 docs/output.md                                |   2 +-
 docs/usage.md                                 |   2 +-
 nextflow_schema.json                          |   2 +-
 .../local/fusioncatcher_workflow/main.nf      |   4 +-
 .../fusioncatcher_workflow/tests/main.nf.test |   2 +-
 tests/test_stub.nf.test                       |   2 +-
 tests/test_stub.nf.test.snap                  | 102 +++++-------------
 workflows/rnafusion.nf                        |  16 +--
 9 files changed, 41 insertions(+), 92 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index ef6b5a3a5..8b5c404aa 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -119,7 +119,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | `--stringtie`         | `--tools stringtie`             |
 | `--all`               | `--tools all`                   |
 |                       | `--fusioncatcher_download_link` |
-|                       | `--tools fusioncatcher_trim`    |
 |                       | `--trim_tail_fusioncatcher`     |
 
 ## v3.0.2 - [2024-04-10]
diff --git a/docs/output.md b/docs/output.md
index ae231e938..6d29b2dc4 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -149,7 +149,7 @@ If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will f
 
 ### Fastp for fusioncatcher
 
-If `--tools fusioncatcher_trim` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This is to potentially shorten the reads fed into `fusioncatcher` further with `--trim_tail_fusioncatcher` if the reads are above 100 bp.
+If `trim_tail_fusioncatcher` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This allows for additional trimming of read tails before running FusionCatcher. For example if reads are 150bp, using --trim_tail_fusioncatcher 50 will shorten reads to 100 bp  by 50 bases from the 3′ end. 100 bp is the recommended read length to feed into FusionCatcher.
 
 <details markdown="1">
 <summary>Output files</summary>
diff --git a/docs/usage.md b/docs/usage.md
index 300d9e320..6a8530c18 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -268,7 +268,7 @@ nextflow run nf-core/rnafusion \
 --adapter_fastq <PATH/TO/ADAPTER/FASTQ> (optional)
 ```
 
-The additional fusioncatcher_trim flag in `--tools` will toggle an additional `fastp` process, especially useful is reads are above 100 bp, which is not handled well by fusioncatcher. The parameter `--trim_tail_fusioncatcher` will trim the extra read length from the tails.
+The additional `--trim_tail_fusioncatcher` flag will toggle an additional `fastp` process, especially useful is reads are above 100 bp, which is not handled well by FusionCatcher. The parameter `--trim_tail_fusioncatcher`need to be provided with the number of bases to remove from the tail end.
 
 #### Filter fusions detected by 2 or more tools
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 239e57a89..a472d333f 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -85,7 +85,7 @@
                     "type": "string",
                     "description": "Comma-delimited list of tools to run",
                     "fa_icon": "far fa-file-code",
-                    "pattern": "^((arriba|ctatsplicing|fusioncatcher|fusioncatcher_trim|starfusion|stringtie|fusionreport|fastp|salmon|fusioninspector|all)?,?)*(?<!,)$"
+                    "pattern": "^((arriba|ctatsplicing|fusioncatcher|starfusion|stringtie|fusionreport|fastp|salmon|fusioninspector|all)?,?)*(?<!,)$"
                 },
                 "read_length": {
                     "type": "integer",
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index b2e5fadd2..856fc63f1 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -8,7 +8,7 @@ include { FUSIONCATCHER_FUSIONCATCHER }      from '../../../modules/nf-core/fusi
 workflow FUSIONCATCHER_WORKFLOW {
     take:
         reads                   // channel [ meta, [ fastqs ] ]
-        fusioncatcher_trim     // boolean
+        fusioncatcher_trimming     // boolean
         adapter_fasta         // channel [ path ]
         fusioncatcher_ref       // channel [ meta, path       ]
         fusioncatcher_fusions   // path, string
@@ -21,7 +21,7 @@ workflow FUSIONCATCHER_WORKFLOW {
             ch_fusioncatcher_fusions = reads.combine(Channel.value(file(fusioncatcher_fusions, checkIfExists:true)))
                                         .map { meta, _reads, fusions -> [ meta, fusions ] }
         } else {
-            if (fusioncatcher_trim) {
+            if (fusioncatcher_trimming) {
                 FASTP_FOR_FUSIONCATCHER(
                     reads,
                     adapter_fasta,
diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
index 05d691889..cca6c6e2d 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test
@@ -35,7 +35,7 @@ nextflow_workflow {
                 """
             }
             params {
-                tools                   = 'fusioncatcher,fusioncatcher_trim'
+                tools                   = 'fusioncatcher'
                 trim_tail_fusioncatcher = 50
                 outdir                  = "$outputDir"
             }
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index b329ed863..f9039416c 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -140,7 +140,7 @@ nextflow_pipeline {
         when {
             params {
                 outdir           = "$outputDir"
-                tools            = "fusioncatcher,fusioncatcher_trim"
+                tools            = "fusioncatcher"
                 trim_tail_fusioncatcher = 50
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
 
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 81d69dd73..db046d4ee 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1191,7 +1191,7 @@
     },
     "stub test fusionreport": {
         "content": [
-            22,
+            23,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1199,6 +1199,9 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "FASTP_FOR_FUSIONCATCHER": {
+                    "fastp": "0.24.0"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1271,6 +1274,12 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "fastp_for_fusioncatcher",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1449,7 +1458,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-16T12:57:56.061136"
+        "timestamp": "2025-06-19T11:14:43.400962"
     },
     "stub test fastp": {
         "content": [
@@ -1526,79 +1535,6 @@
         "timestamp": "2025-06-16T09:26:06.949386"
     },
     "stub test fusioncatcher": {
-        "content": [
-            10,
-            {
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
-                },
-                "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
-            [
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "fusioncatcher",
-                "fusioncatcher/test.fusion-genes.txt",
-                "fusioncatcher/test.log",
-                "fusioncatcher/test.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
-                "references",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-13T19:51:03.177928"
-    },
-    "stub test fusioncatcher trim": {
         "content": [
             11,
             {
@@ -1678,7 +1614,21 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-16T13:27:39.239583"
+        "timestamp": "2025-06-19T11:08:43.632179"
+    },
+    "stub test fusioncatcher trim": {
+        "content": [
+            0,
+            null,
+            [
+                "pipeline_info"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
+        },
+        "timestamp": "2025-06-19T11:09:09.500124"
     },
     "stub test all": {
         "content": [
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 5d2eaa02b..24799b728 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -255,14 +255,14 @@ workflow RNAFUSION {
         def ch_fusioncatcher_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
             fusions_created = true
-            fusioncatcher_trimming = params.tools.contains('fusioncatcher_trim') ?: false
-            FUSIONCATCHER_WORKFLOW (
-                ch_reads,
-                fusioncatcher_trimming,
-                params.adapter_fasta,
-                BUILD_REFERENCES.out.fusioncatcher_ref,       // channel [ meta, path       ]
-                params.fusioncatcher_fusions
-            )
+        fusioncatcher_trimming = { params.trim_tail_fusioncatcher != 0 }
+        FUSIONCATCHER_WORKFLOW (
+            ch_reads,
+            fusioncatcher_trimming,
+            params.adapter_fasta,
+            BUILD_REFERENCES.out.fusioncatcher_ref,       // channel [ meta, path       ]
+            params.fusioncatcher_fusions
+        )
             ch_versions = ch_versions.mix(FUSIONCATCHER_WORKFLOW.out.versions)
             // Add output of fusioncatcher to a channel + add empty entries for the samples that could not be run
             ch_fusioncatcher_fusions = FUSIONCATCHER_WORKFLOW.out.fusions.mix(ch_fastqs_to_process.not_found)

From 5f4ce10d6fac5bd0b06eff891441ee51afb53024 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 11:21:42 +0200
Subject: [PATCH 548/887] prettier

---
 docs/output.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index 6d29b2dc4..dbccd397f 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -149,7 +149,7 @@ If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will f
 
 ### Fastp for fusioncatcher
 
-If `trim_tail_fusioncatcher` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This allows for additional trimming of read tails before running FusionCatcher. For example if reads are 150bp, using --trim_tail_fusioncatcher 50 will shorten reads to 100 bp  by 50 bases from the 3′ end. 100 bp is the recommended read length to feed into FusionCatcher.
+If `trim_tail_fusioncatcher` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This allows for additional trimming of read tails before running FusionCatcher. For example if reads are 150bp, using --trim_tail_fusioncatcher 50 will shorten reads to 100 bp by 50 bases from the 3′ end. 100 bp is the recommended read length to feed into FusionCatcher.
 
 <details markdown="1">
 <summary>Output files</summary>

From 728015ec579339fb6ff9ad5dfe224421a4c8b71c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 11:33:58 +0200
Subject: [PATCH 549/887] update tests and fix issues

---
 .../fusionreport_workflow/tests/main.nf.test  | 27 ++----
 .../tests/main.nf.test.snap                   | 10 +--
 tests/test_stub.nf.test.snap                  | 89 +++++++++++++++++--
 3 files changed, 94 insertions(+), 32 deletions(-)

diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
index 18a6c23e4..c5e2a54ca 100644
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
@@ -11,19 +11,10 @@ nextflow_workflow {
     test("FUSIONREPORT_WORKFLOW - Full Test") {
 
         setup {
-            run("FUSIONCATCHER_FUSIONCATCHER") {
-                script "../../../../modules/nf-core/fusioncatcher/detect/main.nf"
+            run("FUSIONREPORT_DOWNLOAD") {
+                script "../../../../modules/local/fusionreport/download/main.nf"
                 process {
                     """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
-                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true),
-                ]
-                input[1] = [
-                    [ id:'reference'],
-                    []
-                ]
                     """
                 }
             }
@@ -32,25 +23,19 @@ nextflow_workflow {
         when {
             workflow {
                 """
-                // Input channels
-                input[0] = Channel.of(
-                    [ [ id:'test_sample' ],
-                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz") ]
-                )
-
-                input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
+                input[0] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
 
-                input[2] = Channel.of(
+                input[1] = Channel.of(
                     [[ id:'test_sample' ],
                         file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv") ]
                 )
 
-                input[3] =  Channel.of(
+                input[2] =  Channel.of(
                     [[ id:'test_sample' ],
                         file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv") ]
                 )
 
-                input[4] =  Channel.of(
+                input[3] =  Channel.of(
                     [[ id:'test_sample' ],
                         file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt") ]
                 )
diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap b/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
index 2c384e5a2..4dd686d8a 100644
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
@@ -22,7 +22,7 @@
                     {
                         "id": "test_sample"
                     },
-                    "test_sample_fusionreport_index.html:md5,3513bcaa58446399c0957db69402d3bd"
+                    "test_sample_fusionreport_index.html:md5,9dfbf94471173bebbd8085e440515e4d"
                 ]
             ],
             [
@@ -30,17 +30,17 @@
                     {
                         "id": "test_sample"
                     },
-                    "test_sample.fusions.csv:md5,49f378c2112d7e0b3b17d9095c79e6bd"
+                    "test_sample.fusions.csv:md5,ee6c6838110d68bc75a13c19dca0a321"
                 ]
             ],
             [
-                "versions.yml:md5,90749dbf8e3e7b259c935eabb8c6ce1e"
+                "versions.yml:md5,5b80da331704f6d2093b7609537222e8"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-01-07T13:31:41.215356596"
+        "timestamp": "2025-06-19T11:32:39.322725"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index db046d4ee..39a65ed93 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1618,21 +1618,89 @@
     },
     "stub test fusioncatcher trim": {
         "content": [
-            0,
-            null,
+            11,
+            {
+                "FASTP_FOR_FUSIONCATCHER": {
+                    "fastp": "0.24.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER_DOWNLOAD": {
+                    "wget": "1.21.4",
+                    "tar": 1.35
+                },
+                "FUSIONCATCHER_FUSIONCATCHER": {
+                    "fusioncatcher": "1.33"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
-                "pipeline_info"
+                "fastp_for_fusioncatcher",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fusioncatcher",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-19T11:09:09.500124"
+        "timestamp": "2025-06-19T11:19:49.213204"
     },
     "stub test all": {
         "content": [
-            34,
+            35,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1652,6 +1720,9 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
+                "FASTP_FOR_FUSIONCATCHER": {
+                    "fastp": "0.24.0"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1768,6 +1839,12 @@
                 "fastp/test.fastp.log",
                 "fastp/test_1.fastp.fastq.gz",
                 "fastp/test_2.fastp.fastq.gz",
+                "fastp_for_fusioncatcher",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -2106,6 +2183,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-16T12:46:03.993646"
+        "timestamp": "2025-06-19T11:24:03.086146"
     }
 }
\ No newline at end of file

From 2ab557df46624ccac268b027279e13f78cb0714e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 11:37:28 +0200
Subject: [PATCH 550/887] update readme

---
 CHANGELOG.md | 1 +
 README.md    | 2 +-
 2 files changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8b5c404aa..1c599c8c4 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -98,6 +98,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - The `FUSIONINSPECTOR` process will no longer fail when no fusions have been found. [#651](https://github.com/nf-core/rnafusion/pull/651)
 - Fixed STAR-Fusion build would fail when downloading Pfam and Dfam resources behind SSL. [#653](https://github.com/nf-core/rnafusion/pull/653)
 - Fix bug in argument handling for FusionInspector [#669](https://github.com/nf-core/rnafusion/pull/669)
+- Fix missing memory unit for fusioncatcher [#674](https://github.com/nf-core/rnafusion/pull/674)
 
 ### Removed
 
diff --git a/README.md b/README.md
index 92dd1aac9..c1a3ca7d5 100644
--- a/README.md
+++ b/README.md
@@ -113,7 +113,7 @@ For more details about the output files and reports, please refer to the
 
 nf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))
 
-## We thank the following people for their help in the development of this pipeline:
+## We thank the following people for their help in the development of this pipeline
 
 - [Phil Ewels](https://github.com/ewels)
 - [Rickard Hammarén](https://github.com/Hammarn)

From 34f2e78352ab11c0bf77d30039f83148c7a9cc08 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 11:38:27 +0200
Subject: [PATCH 551/887] formatting

---
 subworkflows/local/fusioncatcher_workflow/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index 856fc63f1..bb878754f 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -8,8 +8,8 @@ include { FUSIONCATCHER_FUSIONCATCHER }      from '../../../modules/nf-core/fusi
 workflow FUSIONCATCHER_WORKFLOW {
     take:
         reads                   // channel [ meta, [ fastqs ] ]
-        fusioncatcher_trimming     // boolean
-        adapter_fasta         // channel [ path ]
+        fusioncatcher_trimming  // boolean
+        adapter_fasta           // channel [ path ]
         fusioncatcher_ref       // channel [ meta, path       ]
         fusioncatcher_fusions   // path, string
 

From fd23d016f1f3568a0758dd588ce208046677062f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 13:41:51 +0200
Subject: [PATCH 552/887] add test config

---
 tests/test_stub_bam.nf.test | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
index 4d83cae01..8f080c808 100644
--- a/tests/test_stub_bam.nf.test
+++ b/tests/test_stub_bam.nf.test
@@ -15,6 +15,9 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                tools            = "all"
+                no_cosmic        = true
+
             }
         }
 
@@ -48,6 +51,8 @@ nextflow_pipeline {
                 tools            = "arriba"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                no_cosmic        = true
+
             }
         }
 
@@ -213,6 +218,7 @@ nextflow_pipeline {
                 tools            = "fusionreport,arriba,starfusion,fusioncatcher"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                no_cosmic        = true
             }
         }
 

From a3a07c7782d643759c2297f08c810169c922be88 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 14:43:49 +0200
Subject: [PATCH 553/887] update tests

---
 modules/local/fusioninspector/main.nf         |   2 +-
 .../local/fusioninspector/tests/main.nf.test  |   7 +-
 .../fusioninspector/tests/main.nf.test.snap   | 143 +----
 tests/test_stub.nf.test.snap                  | 488 +++++++++---------
 4 files changed, 252 insertions(+), 388 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 7f1f18278..588debb57 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -13,7 +13,7 @@ process FUSIONINSPECTOR {
 
     output:
     tuple val(meta), path("*FusionInspector.fusions.tsv")                  , emit: tsv
-    tuple val(meta), path("*.gtf")                          , optional:true, emit: out_gtf
+    tuple val(meta), path("fi_workdir/*.gtf")                              , optional:true, emit: out_gtf
     tuple val(meta), path("*FusionInspector.log")                          , emit: log
     tuple val(meta), path("*html")                                         , emit: html
     tuple val(meta), path("*abridged.tsv")                                 , emit: abridged_tsv
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 529b14cc5..f4f82814f 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -98,8 +98,9 @@ nextflow_process {
                 { assert process.success },
                 { assert snapshot(
                     file(process.out.tsv[0][1]).name,
-                    process.out.tsv_coding_effect,  // TODO: These files do not seem to be produced. Consult Annick.
-                    process.out.out_gtf,            // TODO: These files do not seem to be produced. Consult Annick.
+                    process.out.tsv,
+                    process.out.out_gtf,
+                    process.out.abridged_tsv,
                     file(process.out.log[0][1]).name,
                     file(process.out.html[0][1]).name,
                     file(process.out.abridged_tsv[0][1]).name,
@@ -109,7 +110,7 @@ nextflow_process {
                     file(process.out.fi_workdir[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
                     process.out.chckpts_dir,
                     process.out.versions
-                    ).match() }
+                ).match() }
             )
         }
 
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 20c0e710d..85de0806e 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -380,153 +380,14 @@
     },
     "FUSIONINSPECTOR": {
         "content": [
-            "test.FusionInspector.fusions.tsv",
-            [
-                
-            ],
-            [
-                
-            ],
-            "FusionInspector.log",
-            "test.fusion_inspector_web.html",
-            "test.FusionInspector.fusions.abridged.tsv",
-            [
-                "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
-                "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
-                "test.fa.fai:md5,15ddfba09f4abf8a15de5eea6c6286ee",
-                "cytoBand.txt:md5,d8a84202fa42d9455222c94ae641c8b4",
-                "test.bed.sorted.bed.gz:md5,a306900912cc4479f06d61bea00a3ea6",
-                "test.bed.sorted.bed.gz.tbi:md5,8c9d524558ade2b82c67d60bcfa0de6b",
-                "test.spanning_reads.bam.bai:md5,6e035943a5c90026bd499e78c7eeed4a",
-                "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusion_inspector_web.json:md5,ebe4faf6b6133b40d6c2847195cf55a9",
-                "test.ROI.bed:md5,671de301af27b3dab40ee224401d3a0e",
-                "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "tracks.json:md5,72f2234c2ad2b7997226283acf040161"
-            ],
-            [
-                "test.fa:md5,a54bec9daad731a086e8533f89d9c2e6",
-                "test.gtf:md5,d523061bd8b443a014d4cca9406ec772",
-                "pipeliner.456.cmds:md5,bb8bf0e749f794dfb37efc2ff7c24be0",
-                "Log.progress.out:md5,b2bd061d6cbaaf3d6d3b1fed547f69b8",
-                [
-                    "exonGeTrInfo.tab:md5,8815dc1fce3a5f6cb836ea8f822e9c81",
-                    "exonInfo.tab:md5,e51c649a0ca61719e8d4f7324c069c60",
-                    "geneInfo.tab:md5,2f334fb0772c906860c9489ee0d8f3ff",
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-                "test.star.sortedByCoord.out.bam.bai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam:md5,7585d76d73eacfc222ce76cdb4e22d0c",
-                "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam:md5,c9e99bbe81bdbadf5fa56176828540ae",
-                "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis:md5,a1a0daca698e0ff4141f820c9fe64360",
-                "test.igv.Pfam.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.igv.seqsimilar.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info:md5,ed6214ba6c0cd135afd76a808dfdbd5d"
-            ],
-            [
-                "test.fa",
-                "test.gtf",
-                "test.fa.fai",
-                "cytoBand.txt",
-                "test.bed.sorted.bed.gz",
-                "test.bed.sorted.bed.gz.tbi",
-                "test.spanning_reads.bam.bai",
-                "test.igv.Pfam.bed",
-                "test.igv.seqsimilar.bed",
-                "test.fusion_inspector_web.json",
-                "test.ROI.bed",
-                "TrinityFusion.bed",
-                "tracks.json"
-            ],
-            [
-                "test.fa",
-                "test.gtf",
-                "pipeliner.456.cmds",
-                "Log.progress.out",
-                "_STARgenome",
-                "SJ.out.tab",
-                "star_align.ok",
-                "test.star.sortedByCoord.out.bam.ok",
-                "test.star.sortedByCoord.out.bam.bai.ok",
-                "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "test.igv.Pfam.gff3",
-                "test.igv.seqsimilar.gff3",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info"
-            ],
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    [
-                        "EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "add_FFPM.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "add_splice_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "append_microH_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "blast_filter.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "coalesce_junc_n_span.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "cp_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "cp_contigs_file_workdir:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "cp_final.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "cp_gtf_file_workdir.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "cp_tracks_json.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "create_fi_igvjs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "cytoband.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "filter_by_frag_threshs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "final.abridged.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_annotator.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_coding_region_effect.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_contigs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "fusion_reports_html.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "get_fusion_JUNCTION_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "get_fusion_SPANNING_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "index_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "init_EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "init_spanning_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "mark_dup_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "mark_dups_reads.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "merged_contig_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "merged_contig_gtf_to_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "microH.dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "prep_igv_extract_junc_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "prep_igv_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "prep_igv_pfam_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "prep_igv_pfam_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "prep_igv_seqsim_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "prep_igv_seqsim_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "prep_spanning_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "run_STAR.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "samtools_idx_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "samtools_index_span_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "span_reads_acc.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.bed.bedsort.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.bed.bgzip.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                        "test.bed.tabix.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ]
-            ],
             [
                 "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2024-12-31T14:34:37.759837761"
+        "timestamp": "2025-06-19T11:55:09.238503"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 824773b56..6c0425162 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -581,124 +581,125 @@
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "fusioninspector",
-                "fusioninspector/IGV_inputs",
-                "fusioninspector/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/IGV_inputs/cytoBand.txt",
-                "fusioninspector/IGV_inputs/test.ROI.bed",
-                "fusioninspector/IGV_inputs/test.bed",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/IGV_inputs/test.fa",
-                "fusioninspector/IGV_inputs/test.fa.fai",
-                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/IGV_inputs/test.gtf",
-                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/IGV_inputs/tracks.json",
-                "fusioninspector/chckpts_dir",
-                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/chckpts_dir/blast_filter.ok",
-                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/chckpts_dir/cp_final.ok",
-                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/chckpts_dir/cytoband.ok",
-                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/chckpts_dir/final.abridged.ok",
-                "fusioninspector/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/chckpts_dir/microH.dat.ok",
-                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/chckpts_dir/run_STAR.ok",
-                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/fi_workdir",
-                "fusioninspector/fi_workdir/Log.final.out",
-                "fusioninspector/fi_workdir/Log.out",
-                "fusioninspector/fi_workdir/Log.progress.out",
-                "fusioninspector/fi_workdir/SJ.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome",
-                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/fi_workdir/microH.dat",
-                "fusioninspector/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/fi_workdir/star_align.ok",
-                "fusioninspector/fi_workdir/test.fa",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/fi_workdir/test.gtf",
-                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.fusion_inspector_web.html",
+                "fusioninspector/test",
+                "fusioninspector/test/IGV_inputs",
+                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/test/IGV_inputs/cytoBand.txt",
+                "fusioninspector/test/IGV_inputs/test.ROI.bed",
+                "fusioninspector/test/IGV_inputs/test.bed",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.fa",
+                "fusioninspector/test/IGV_inputs/test.fa.fai",
+                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/test/IGV_inputs/test.gtf",
+                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/tracks.json",
+                "fusioninspector/test/chckpts_dir",
+                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/test/chckpts_dir/blast_filter.ok",
+                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/test/chckpts_dir/cp_final.ok",
+                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/test/chckpts_dir/cytoband.ok",
+                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/test/chckpts_dir/final.abridged.ok",
+                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/test/chckpts_dir/microH.dat.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/test/chckpts_dir/run_STAR.ok",
+                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/test/fi_workdir",
+                "fusioninspector/test/fi_workdir/Log.final.out",
+                "fusioninspector/test/fi_workdir/Log.out",
+                "fusioninspector/test/fi_workdir/Log.progress.out",
+                "fusioninspector/test/fi_workdir/SJ.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/test/fi_workdir/microH.dat",
+                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/test/fi_workdir/star_align.ok",
+                "fusioninspector/test/fi_workdir/test.fa",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/test/fi_workdir/test.gtf",
+                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test/test.FusionInspector.log",
+                "fusioninspector/test/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -863,10 +864,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T14:06:10.151871018"
+        "timestamp": "2025-06-19T14:09:35.887706"
     },
     "stub test salmon": {
         "content": [
@@ -1747,124 +1748,125 @@
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
                 "fusioninspector",
-                "fusioninspector/IGV_inputs",
-                "fusioninspector/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/IGV_inputs/cytoBand.txt",
-                "fusioninspector/IGV_inputs/test.ROI.bed",
-                "fusioninspector/IGV_inputs/test.bed",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/IGV_inputs/test.fa",
-                "fusioninspector/IGV_inputs/test.fa.fai",
-                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/IGV_inputs/test.gtf",
-                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/IGV_inputs/tracks.json",
-                "fusioninspector/chckpts_dir",
-                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/chckpts_dir/blast_filter.ok",
-                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/chckpts_dir/cp_final.ok",
-                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/chckpts_dir/cytoband.ok",
-                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/chckpts_dir/final.abridged.ok",
-                "fusioninspector/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/chckpts_dir/microH.dat.ok",
-                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/chckpts_dir/run_STAR.ok",
-                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/fi_workdir",
-                "fusioninspector/fi_workdir/Log.final.out",
-                "fusioninspector/fi_workdir/Log.out",
-                "fusioninspector/fi_workdir/Log.progress.out",
-                "fusioninspector/fi_workdir/SJ.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome",
-                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/fi_workdir/microH.dat",
-                "fusioninspector/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/fi_workdir/star_align.ok",
-                "fusioninspector/fi_workdir/test.fa",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/fi_workdir/test.gtf",
-                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.fusion_inspector_web.html",
+                "fusioninspector/test",
+                "fusioninspector/test/IGV_inputs",
+                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/test/IGV_inputs/cytoBand.txt",
+                "fusioninspector/test/IGV_inputs/test.ROI.bed",
+                "fusioninspector/test/IGV_inputs/test.bed",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.fa",
+                "fusioninspector/test/IGV_inputs/test.fa.fai",
+                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/test/IGV_inputs/test.gtf",
+                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/tracks.json",
+                "fusioninspector/test/chckpts_dir",
+                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/test/chckpts_dir/blast_filter.ok",
+                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/test/chckpts_dir/cp_final.ok",
+                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/test/chckpts_dir/cytoband.ok",
+                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/test/chckpts_dir/final.abridged.ok",
+                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/test/chckpts_dir/microH.dat.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/test/chckpts_dir/run_STAR.ok",
+                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/test/fi_workdir",
+                "fusioninspector/test/fi_workdir/Log.final.out",
+                "fusioninspector/test/fi_workdir/Log.out",
+                "fusioninspector/test/fi_workdir/Log.progress.out",
+                "fusioninspector/test/fi_workdir/SJ.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/test/fi_workdir/microH.dat",
+                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/test/fi_workdir/star_align.ok",
+                "fusioninspector/test/fi_workdir/test.fa",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/test/fi_workdir/test.gtf",
+                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test/test.FusionInspector.log",
+                "fusioninspector/test/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -2071,9 +2073,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T14:01:35.330928392"
+        "timestamp": "2025-06-19T13:51:57.84272"
     }
-}
+}
\ No newline at end of file

From 3924b8185b20e7b610554c4aac86360c27c5deb2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 19 Jun 2025 15:08:12 +0200
Subject: [PATCH 554/887] update snapshot

---
 tests/test_stub_bam.nf.test.snap | 125 +++++++++++--------------------
 1 file changed, 43 insertions(+), 82 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 88115a4b6..9d487f4f5 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -419,72 +419,6 @@
     "stub test fusioninspector": {
         "content": [
             21,
-            {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
-                },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
-                },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.4.0"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
-                },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
-                },
-                "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "HGNC_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
             [
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
@@ -761,10 +695,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T13:30:43.376109569"
+        "timestamp": "2025-06-19T14:44:48.727739"
     },
     "stub test salmon": {
         "content": [
@@ -1084,7 +1018,7 @@
     },
     "stub test fusionreport": {
         "content": [
-            20,
+            21,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1092,6 +1026,9 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "FASTP_FOR_FUSIONCATCHER": {
+                    "fastp": "0.24.0"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1156,6 +1093,12 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "fastp_for_fusioncatcher",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1312,10 +1255,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T13:58:03.351087941"
+        "timestamp": "2025-06-19T13:32:03.861817"
     },
     "stub test fastp": {
         "content": [
@@ -1412,8 +1355,11 @@
     },
     "stub test fusioncatcher": {
         "content": [
-            13,
+            14,
             {
+                "FASTP_FOR_FUSIONCATCHER": {
+                    "fastp": "0.24.0"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1457,6 +1403,12 @@
                 }
             },
             [
+                "fastp_for_fusioncatcher",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1497,14 +1449,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T13:27:50.155561081"
+        "timestamp": "2025-06-19T13:03:53.381632"
     },
     "stub test all": {
         "content": [
-            33,
+            34,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1524,6 +1476,9 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
+                "FASTP_FOR_FUSIONCATCHER": {
+                    "fastp": "0.24.0"
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1632,6 +1587,12 @@
                 "fastp/test.fastp.log",
                 "fastp/test_1.fastp.fastq.gz",
                 "fastp/test_2.fastp.fastq.gz",
+                "fastp_for_fusioncatcher",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
+                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1951,9 +1912,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T13:26:09.703953189"
+        "timestamp": "2025-06-19T12:58:38.773344"
     }
-}
+}
\ No newline at end of file

From bbaa53f983b3aff08a6899605dee7208589d5c22 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 30 Jun 2025 09:54:53 +0200
Subject: [PATCH 555/887] add snap

---
 .../fusioninspector/tests/main.nf.test.snap    | 18 +++++++++++++++---
 tests/test_stub.nf.test.snap                   | 11 ++---------
 2 files changed, 17 insertions(+), 12 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 85de0806e..f88722184 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -12,7 +12,13 @@
                     ]
                 ],
                 "1": [
-                    
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "2": [
                     [
@@ -356,7 +362,13 @@
                     ]
                 ],
                 "out_gtf": [
-                    
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
                 "tsv": [
                     [
@@ -376,7 +388,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-19T14:11:46.597061"
+        "timestamp": "2025-06-19T15:26:31.711939"
     },
     "FUSIONINSPECTOR": {
         "content": [
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 6c0425162..e7f5410a5 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1601,7 +1601,7 @@
     },
     "stub test all": {
         "content": [
-            34,
+            33,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1609,9 +1609,6 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.4.0"
-                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -1880,10 +1877,6 @@
                 "get",
                 "get/rrna.bed",
                 "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
                 "picard",
                 "picard/test.bai",
                 "picard/test.bam",
@@ -2076,6 +2069,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-19T13:51:57.84272"
+        "timestamp": "2025-06-19T15:22:04.26107"
     }
 }
\ No newline at end of file

From 3244726a52cc8c8fb27a13ca2b45f6559c8efa11 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 30 Jun 2025 10:53:46 +0200
Subject: [PATCH 556/887] update container

---
 modules/local/starfusion/build/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 98bf43ce3..f4548af7b 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -4,8 +4,8 @@ process STARFUSION_BUILD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/47/47bad9870d9b98e40cd0210edd20fcc018f55098a49b7c8d00aa7e90f88c5e5c/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:10b5c8c209d8edaa'}"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3f/3f616513213e4f4f71ff5a70022f2a8f19e3d581afbe7a3f21fe30b7ed2d6d2a/data' :
+        'community.wave.seqera.io/library/minimap2_star-fusion:6156774e85366463'}"
 
 
     input:

From e7520a43d69a113f16ecc3b77b5655e3021eb084 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 30 Jun 2025 11:20:38 +0200
Subject: [PATCH 557/887] update container

---
 modules/local/starfusion/build/main.nf | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index f4548af7b..ac450d8f5 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,9 +3,7 @@ process STARFUSION_BUILD {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3f/3f616513213e4f4f71ff5a70022f2a8f19e3d581afbe7a3f21fe30b7ed2d6d2a/data' :
-        'community.wave.seqera.io/library/minimap2_star-fusion:6156774e85366463'}"
+    container 'docker.io/trinityctat/starfusion:1.15.0'
 
 
     input:

From 35e91bdfd0fa89c12ae13f186f514a278f9d3421 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 30 Jun 2025 15:12:40 +0200
Subject: [PATCH 558/887] update snap

---
 modules/local/fusioninspector/main.nf         |  4 +-
 .../local/fusioninspector/tests/main.nf.test  | 53 +------------------
 .../fusioninspector/tests/main.nf.test.snap   | 43 +++++++++++++--
 modules/local/starfusion/build/main.nf        |  4 +-
 .../local/starfusion/build/tests/main.nf.test |  1 -
 5 files changed, 43 insertions(+), 62 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 588debb57..54a546983 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -41,7 +41,7 @@ process FUSIONINSPECTOR {
         --vis $args $args2
 
     # Touch the output files to make sure they exist
-    touch ${prefix}.FusionInspector.log
+    touch FusionInspector.log
     touch ${prefix}.FusionInspector.fusions.abridged.tsv
     touch ${prefix}.FusionInspector.fusions.tsv
     touch ${prefix}.fusion_inspector_web.html
@@ -58,7 +58,7 @@ process FUSIONINSPECTOR {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    touch ${prefix}.FusionInspector.log
+    touch FusionInspector.log
     touch ${prefix}.FusionInspector.fusions.abridged.tsv
     touch ${prefix}.FusionInspector.fusions.tsv
     touch ${prefix}.fusion_inspector_web.html
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index f4f82814f..65a190789 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -52,64 +52,13 @@ nextflow_process {
         }
 
         then {
-
-            def unstableFiles = [
-                "test.bed",
-                "test.consolidated.bam",
-                "test.consolidated.bam.bai",
-                "test.junction_reads.bam",
-                "test.junction_reads.bam.bai",
-                "test.spanning_reads.bam",
-                "Log.final.out",
-                "Log.out",
-                "microH.dat",
-                "test.fusion_preds.coalesced.summary",
-                "test.fusion_preds.coalesced.summary.EMadj",
-                "test.fusion_preds.coalesced.summary.min_frag_thresh",
-                "test.fusion_preds.coalesced.summary.wSpliceInfo",
-                "test.fusion_preds.coalesced.summary.wSpliceInfo.post_blast_filter",
-                "test.fusion_preds.coalesced.summary.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "test.fusion_preds.coalesced.summary.fusion_junction_reads_acc",
-                "test.fusion_preds.coalesced.summary.fusion_spanning_reads_acc",
-                "test.post_blast_and_promiscuity_filter",
-                "test.post_blast_and_promiscuity_filter.EMadj",
-                "test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "test.star.cSorted.dupsMarked.bam",
-                "test.star.cSorted.dupsMarked.bam.bai",
-                "test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "test.star.cSorted.dupsMarked.bam.read_align_counts",
-                "test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "test.star.sortedByCoord.out.bam",
-                "test.star.sortedByCoord.out.bam.bai",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter"
-            ]
-
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    file(process.out.tsv[0][1]).name,
                     process.out.tsv,
                     process.out.out_gtf,
                     process.out.abridged_tsv,
-                    file(process.out.log[0][1]).name,
-                    file(process.out.html[0][1]).name,
-                    file(process.out.abridged_tsv[0][1]).name,
-                    file(process.out.igv_inputs[0][1]).listFiles().findAll { file -> !unstableFiles.contains(file.name) },
-                    file(process.out.fi_workdir[0][1]).listFiles().findAll { file -> !unstableFiles.contains(file.name) },
-                    file(process.out.igv_inputs[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
-                    file(process.out.fi_workdir[0][1]).list().findAll { file -> !unstableFiles.contains(file) },
-                    process.out.chckpts_dir,
-                    process.out.versions
+                    process.out.log,
                 ).match() }
             )
         }
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index f88722184..44a554689 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -26,7 +26,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "3": [
@@ -358,7 +358,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "out_gtf": [
@@ -388,18 +388,51 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-19T15:26:31.711939"
+        "timestamp": "2025-06-30T15:11:46.208438"
     },
     "FUSIONINSPECTOR": {
         "content": [
             [
-                "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.FusionInspector.fusions.tsv:md5,01a2578b59f112d23a1475cd75b9af0b"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.gtf:md5,d523061bd8b443a014d4cca9406ec772"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.FusionInspector.fusions.abridged.tsv:md5,98d99b567527d990086689e4f7f8cc12"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "FusionInspector.log:md5,6cdafdbfe281ca9bb92c484e5a001d41"
+                ]
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-19T11:55:09.238503"
+        "timestamp": "2025-06-30T15:11:10.528898"
     }
 }
\ No newline at end of file
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index ac450d8f5..6697b2ba3 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -20,6 +20,7 @@ process STARFUSION_BUILD {
 
     script:
     def args = task.ext.args ?: ''
+    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
     """
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${pfam_version}/Pfam-A.hmm.gz --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm --no-check-certificate
@@ -30,8 +31,7 @@ process STARFUSION_BUILD {
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
 
-
-    prep_genome_lib.pl \\
+    $binPath \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --dfam_db ${dfam_species}_dfam.hmm \\
diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
index 07777230e..56e2bea3d 100644
--- a/modules/local/starfusion/build/tests/main.nf.test
+++ b/modules/local/starfusion/build/tests/main.nf.test
@@ -94,7 +94,6 @@ nextflow_process {
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/genomeParameters.txt").exists(),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Log.out").exists(),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),

From aecb27d85e08bbe43dad48ca8272a0d501cd5a41 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 30 Jun 2025 15:15:38 +0200
Subject: [PATCH 559/887] update container

---
 modules/local/starfusion/build/tests/main.nf.test | 1 +
 modules/local/starfusion/detect/main.nf           | 4 +---
 2 files changed, 2 insertions(+), 3 deletions(-)

diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
index 56e2bea3d..07777230e 100644
--- a/modules/local/starfusion/build/tests/main.nf.test
+++ b/modules/local/starfusion/build/tests/main.nf.test
@@ -94,6 +94,7 @@ nextflow_process {
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/genomeParameters.txt").exists(),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Log.out").exists(),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
                     path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index aee6fe12e..a2dc6df03 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -3,9 +3,7 @@ process STARFUSION {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/47/47bad9870d9b98e40cd0210edd20fcc018f55098a49b7c8d00aa7e90f88c5e5c/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:10b5c8c209d8edaa'}"
+    container 'docker.io/trinityctat/starfusion:1.15.0'
 
     input:
     tuple val(meta), path(reads), path(junction)

From b547a9c68b0553e5fd1b2a92946ea8ae804310ca Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Jul 2025 11:58:59 +0200
Subject: [PATCH 560/887] update to 1.15.1

---
 modules/local/starfusion/build/main.nf  | 4 +++-
 modules/local/starfusion/detect/main.nf | 4 +++-
 2 files changed, 6 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index 6697b2ba3..b10c7d7e2 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -3,7 +3,9 @@ process STARFUSION_BUILD {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'docker.io/trinityctat/starfusion:1.15.0'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/75/75d085bf2a8e40c6693b357800eef0f9568f661226d0888339bc77f7852234bb/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:e285bb3eb373b9a7'}"
 
 
     input:
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index a2dc6df03..64b106325 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -3,7 +3,9 @@ process STARFUSION {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container 'docker.io/trinityctat/starfusion:1.15.0'
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/75/75d085bf2a8e40c6693b357800eef0f9568f661226d0888339bc77f7852234bb/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:e285bb3eb373b9a7'}"
 
     input:
     tuple val(meta), path(reads), path(junction)

From cfde716f45c70cdf1fa562fc1c5d46ae849df9f6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Jul 2025 12:54:37 +0200
Subject: [PATCH 561/887] reverse binpath

---
 modules/local/starfusion/build/main.nf | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
index b10c7d7e2..1f49df237 100644
--- a/modules/local/starfusion/build/main.nf
+++ b/modules/local/starfusion/build/main.nf
@@ -7,7 +7,6 @@ process STARFUSION_BUILD {
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/75/75d085bf2a8e40c6693b357800eef0f9568f661226d0888339bc77f7852234bb/data' :
         'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:e285bb3eb373b9a7'}"
 
-
     input:
     tuple val(meta), path(fasta)
     tuple val(meta2), path(gtf)
@@ -22,7 +21,6 @@ process STARFUSION_BUILD {
 
     script:
     def args = task.ext.args ?: ''
-    def binPath = (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1)  ? "prep_genome_lib.pl" : "/usr/local/src/STAR-Fusion/ctat-genome-lib-builder/prep_genome_lib.pl"
     """
     wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${pfam_version}/Pfam-A.hmm.gz --no-check-certificate
     wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm --no-check-certificate
@@ -33,7 +31,7 @@ process STARFUSION_BUILD {
     gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
     wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
 
-    $binPath \\
+    prep_genome_lib.pl \\
         --genome_fa $fasta \\
         --gtf $gtf \\
         --dfam_db ${dfam_species}_dfam.hmm \\

From ddd90b4efcc92906121e54be98ca484f7a7c1a3b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Jul 2025 13:32:30 +0200
Subject: [PATCH 562/887] update snap

---
 tests/test_stub_bam.nf.test.snap | 68 +++++++++++++++++++++++++++++++-
 1 file changed, 67 insertions(+), 1 deletion(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 9d487f4f5..bcb2720bb 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -419,6 +419,72 @@
     "stub test fusioninspector": {
         "content": [
             21,
+            {
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.4.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.4.0"
+                },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.4.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONINSPECTOR": {
+                    "STAR-Fusion": null
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "4.0.0"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "4.0.0"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
+                    "STAR-Fusion": "1.15.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
@@ -698,7 +764,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-19T14:44:48.727739"
+        "timestamp": "2025-07-02T13:32:16.607819"
     },
     "stub test salmon": {
         "content": [

From 32388a7bec0736503d2313a546f8fbc28b5c1fb5 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 2 Jul 2025 12:50:07 +0000
Subject: [PATCH 563/887] refact: simplify pipeline by removing subworkflow
 containing only one module

---
 CHANGELOG.md                                  |   1 +
 conf/test_build.config                        |   4 +-
 subworkflows/local/arriba_workflow/main.nf    |  44 --
 .../local/arriba_workflow/tests/main.nf.test  | 390 ------------------
 .../arriba_workflow/tests/main.nf.test.snap   |  57 ---
 workflows/rnafusion.nf                        |  38 +-
 6 files changed, 26 insertions(+), 508 deletions(-)
 delete mode 100644 subworkflows/local/arriba_workflow/main.nf
 delete mode 100644 subworkflows/local/arriba_workflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/arriba_workflow/tests/main.nf.test.snap

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 94c5a7d6b..3277e3f06 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -104,6 +104,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed test-build as reference building gets integrated in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Removed parameter `--build_references`
 - Removed fusioncatcher build [#650](https://github.com/nf-core/rnafusion/pull/650)
+- Removed subworkflow with less than two modules: `ARRIBA_WORKFLOW` [#xxx](https://github.com/nf-core/rnafusion/pull/xxx)
 
 ### Parameters
 
diff --git a/conf/test_build.config b/conf/test_build.config
index a483ed658..5fcf4c478 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -50,8 +50,8 @@ params {
 // Limit and standardize resources for github actions and reproducibility
 process {
     resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
+        cpus: 8,
+        memory: '16.GB',
         time: '1.h'
     ]
 }
diff --git a/subworkflows/local/arriba_workflow/main.nf b/subworkflows/local/arriba_workflow/main.nf
deleted file mode 100644
index 7580133b8..000000000
--- a/subworkflows/local/arriba_workflow/main.nf
+++ /dev/null
@@ -1,44 +0,0 @@
-include { ARRIBA_ARRIBA                               } from '../../../modules/nf-core/arriba/arriba/main'
-
-workflow ARRIBA_WORKFLOW {
-    take:
-        reads                           // channel [ meta, [ bam ]            ]
-        ch_gtf                          // channel [ meta, path_gtf           ]
-        ch_fasta                        // channel [ meta, path_fasta         ]
-        ch_arriba_ref_blacklist         // channel [ meta, path_blacklist     ]
-        ch_arriba_ref_cytobands         // channel [ meta, path_cytobands     ]
-        ch_arriba_ref_known_fusions     // channel [ meta, path_known_fusions ]
-        ch_arriba_ref_protein_domains   // channel [ meta, path_proteins      ]
-        arriba_fusions                  // path
-
-    main:
-
-        def ch_versions           = Channel.empty()
-        def ch_arriba_fusions     = Channel.empty()
-        def ch_arriba_fusion_fail = Channel.empty()
-
-        if (arriba_fusions) {
-            ch_arriba_fusions = reads.combine( Channel.value( file( arriba_fusions, checkIfExists: true ) ) )
-                .map { it -> [ it[0], it[2] ] }
-        } else {
-            ARRIBA_ARRIBA (
-                reads,
-                ch_fasta,
-                ch_gtf,
-                ch_arriba_ref_blacklist,
-                ch_arriba_ref_known_fusions,
-                ch_arriba_ref_cytobands,
-                ch_arriba_ref_protein_domains
-            )
-            ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
-
-            ch_arriba_fusions     = ARRIBA_ARRIBA.out.fusions
-            ch_arriba_fusion_fail = ARRIBA_ARRIBA.out.fusions_fail.map{ it -> return it[1] }
-        }
-
-    emit:
-        fusions      = ch_arriba_fusions        // channel [ meta, path_fusions   ]
-        fusions_fail = ch_arriba_fusion_fail    // channel [ path, fusions_failed ]
-        versions     = ch_versions              // channel [ versions             ]
-    }
-
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
deleted file mode 100644
index e49a3768c..000000000
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test
+++ /dev/null
@@ -1,390 +0,0 @@
-nextflow_workflow {
-
-    name "Test Subworkflow ARRIBA_WORKFLOW"
-    script "../main.nf"
-    workflow "ARRIBA_WORKFLOW"
-    tag "subworkflow"
-    tag "arriba"
-    tag "arriba/arriba"
-    tag "samtools"
-    tag "samtools/index"
-    tag "samtools/sort"
-    tag "samtools/view"
-    tag "star"
-    tag "star/genomegenerate"
-    tag "star/align"
-
-
-    // Test #1 Indexing
-    test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4") {
-
-        setup {
-            // Create genome index for STAR
-            run("STAR_GENOMEGENERATE") {
-                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
-                process {
-                """
-                    // FASTA
-                    input[0] = Channel.fromPath(
-                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-
-                    // GTF
-                    input[1] = Channel.fromPath(
-                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-                    """
-                }
-            }
-        }
-
-        when {
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [ id: "test_fastqs" ],
-                        [
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
-                        ]
-                    ] )
-
-                // ch_gtf
-                input[1] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_fasta
-                input[2] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_starindex_ref
-                input[3] = STAR_GENOMEGENERATE.out.index
-
-                // ch_arriba_ref_blacklist
-                input[4] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_known_fusions
-                input[5] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_cytobands
-                input[6] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_protein_domains
-                input[7] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // arriba (boolean)
-                input[8] = true
-
-                // all (boolean)
-                input[9] = true
-
-                // fusioninspector_only  (boolean)
-                input[10] = false
-
-                // star_ignore_sjdbgtf (boolean)
-                input[11] = false
-
-                // seq_center (string)
-                input[12] = 'test_center'
-
-                // arriba_fusions (path)
-                input[13] = null
-
-                // cram (array)
-                input[14] = [ 'arriba' ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { with(workflow.out) {
-                    assert snapshot(
-                            file(fusions[0][1]),
-                            file(fusions_fail[0]),
-                            file(cram_index[0][1]).name,
-                            file(cram_index[0][2]).name,
-                            versions.collect{ file(it) }
-                        ).match()
-                    }
-                }
-            )
-        }
-    }
-
-
-    // Test #2 With arriba_fusions file
-    test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - External fusion file") {
-
-        setup {
-            // Create genome index for STAR
-            run("STAR_GENOMEGENERATE") {
-                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
-                process {
-                """
-                    // FASTA
-                    input[0] = Channel.fromPath(
-                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-
-                    // GTF
-                    input[1] = Channel.fromPath(
-                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-                    """
-                }
-            }
-        }
-
-        when {
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [ id: "test_fastqs" ],
-                        [
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
-                        ]
-                    ] )
-
-                // ch_gtf
-                input[1] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_fasta
-                input[2] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_starindex_ref
-                input[3] = STAR_GENOMEGENERATE.out.index
-
-                // ch_arriba_ref_blacklist
-                input[4] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_known_fusions
-                input[5] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_cytobands
-                input[6] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_protein_domains
-                input[7] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // arriba (boolean)
-                input[8] = true
-
-                // all (boolean)
-                input[9] = true
-
-                // fusioninspector_only  (boolean)
-                input[10] = false
-
-                // star_ignore_sjdbgtf (boolean)
-                input[11] = false
-
-                // seq_center (string)
-                input[12] = 'test_center'
-
-                // arriba_fusions (string path)
-                input[13] = "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv"
-
-                // cram (array)
-                input[14] = [ 'arriba' ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { with(workflow.out) {
-                    assert snapshot(
-                            fusions[0].size() == 2,
-                            fusions_fail.size() == 1,
-                            file(cram_index[0][1]).name,
-                            file(cram_index[0][2]).name,
-                            versions.collect{ file(it) }
-                        ).match()
-                    }
-                }
-            )
-        }
-    }
-
-    // TEST #3 WITHOUT INDEXING
-    test("ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - cram = []") {
-
-        setup {
-            // Create genome index for STAR
-            run("STAR_GENOMEGENERATE") {
-                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
-                process {
-                """
-                    // FASTA
-                    input[0] = Channel.fromPath(
-                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-
-                    // GTF
-                    input[1] = Channel.fromPath(
-                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-                    """
-                }
-            }
-        }
-
-        when {
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [ id: "test_fastqs" ],
-                        [
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_1.fq.gz", checkIfExists: true),
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/human/reads_2.fq.gz", checkIfExists: true)
-                        ]
-                    ] )
-
-                // ch_gtf
-                input[1] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_fasta
-                input[2] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.1700000-54900000.fa", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_starindex_ref
-                input[3] = STAR_GENOMEGENERATE.out.index
-
-                // ch_arriba_ref_blacklist
-                input[4] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_known_fusions
-                input[5] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_cytobands
-                input[6] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_arriba_ref_protein_domains
-                input[7] =
-                    Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // arriba (boolean)
-                input[8] = true
-
-                // all (boolean)
-                input[9] = true
-
-                // fusioninspector_only  (boolean)
-                input[10] = false
-
-                // star_ignore_sjdbgtf (boolean)
-                input[11] = false
-
-                // seq_center (string)
-                input[12] = 'test_center'
-
-                // arriba_fusions (path)
-                input[13] = null
-
-                // cram (array)
-                input[14] = [ ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { with(workflow.out) {
-                    assert snapshot(
-                            file(fusions[0][1]),
-                            file(fusions_fail[0]),
-                            cram_index.size() == 0,
-                            versions.collect{ file(it) }
-                        ).match()
-                    }
-                }
-            )
-        }
-    }
-
-}
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap b/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 2057827a2..000000000
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,57 +0,0 @@
-{
-    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - External fusion file": {
-        "content": [
-            true,
-            true,
-            "test_fastqs_star_for_arriba_sorted.cram",
-            "test_fastqs_star_for_arriba_sorted.cram.crai",
-            [
-                "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
-                "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
-                "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
-                "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-11T15:53:59.18258718"
-    },
-    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4": {
-        "content": [
-            "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d",
-            "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98",
-            "test_fastqs_star_for_arriba_sorted.cram",
-            "test_fastqs_star_for_arriba_sorted.cram.crai",
-            [
-                "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
-                "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9",
-                "versions.yml:md5,85458747b55f37c1a5afd39ee7a3a4aa",
-                "versions.yml:md5,bfc5d96804f2991c7f7c705f1ddf81ec",
-                "versions.yml:md5,f4f64d3f1fd867d5afa51e03f7cf2824"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-11T15:43:48.053656601"
-    },
-    "ARRIBA_WORKFLOW - Homo sapiens - FASTQs chr4 - cram = []": {
-        "content": [
-            "test_fastqs.arriba.fusions.tsv:md5,8f39789c4428e81eb9a8d0e54c34c43d",
-            "test_fastqs.arriba.fusions.discarded.tsv:md5,b804c1ed5b01d34163f5c0b2f6810f98",
-            true,
-            [
-                "versions.yml:md5,24030f38976402fad0861e6ec99ee6b6",
-                "versions.yml:md5,439bbb92ff0a83f1e278fc396e9d8ce9"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-11T16:07:37.079418154"
-    }
-}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index bda6500af..1194c1192 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -7,7 +7,6 @@
 include { BUILD_REFERENCES              }   from '../subworkflows/local/build_references'
 include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
 include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow/main'
-include { ARRIBA_WORKFLOW               }   from '../subworkflows/local/arriba_workflow'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
 include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
 include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
@@ -26,6 +25,7 @@ include { paramsSummaryMultiqc          }   from '../subworkflows/nf-core/utils_
 include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
+include { ARRIBA_ARRIBA                 }   from '../modules/nf-core/arriba/arriba/main'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -209,29 +209,37 @@ workflow RNAFUSION {
         }
 
         //
-        // SUBWORKFLOW: Run Arriba
+        // MODULE: Run Arriba
         //
 
         // TODO: improve how params.arriba_fusions would avoid running arriba module. Maybe imputed from samplesheet?
 
         def fusions_created = false
         def ch_arriba_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set arriba fusions to empty by default
-        if(tools.contains("arriba")) {
+
+        if (tools.contains("arriba")) {
             fusions_created = true
-            ARRIBA_WORKFLOW (
-                ch_aligned_reads.map { meta, bam, _bai -> [ meta, bam ]},
-                BUILD_REFERENCES.out.gtf,
-                BUILD_REFERENCES.out.fasta,
-                BUILD_REFERENCES.out.arriba_ref_blacklist,
-                BUILD_REFERENCES.out.arriba_ref_cytobands,
-                BUILD_REFERENCES.out.arriba_ref_known_fusions,
-                BUILD_REFERENCES.out.arriba_ref_protein_domains,
-                params.arriba_fusions
-            )
-            ch_versions = ch_versions.mix(ARRIBA_WORKFLOW.out.versions)
-            ch_arriba_fusions = ARRIBA_WORKFLOW.out.fusions
+
+            if (params.arriba_fusions) {
+                ch_arriba_fusions = ch_aligned_reads.map { meta, _bam, _bai -> meta }
+                    .combine(Channel.value(file(params.arriba_fusions, checkIfExists: true)))
+                    .map { meta, fusion_file -> [meta, fusion_file] }
+            } else {
+                ARRIBA_ARRIBA(
+                    ch_aligned_reads.map { meta, bam, _bai -> [meta, bam] },
+                    BUILD_REFERENCES.out.fasta,
+                    BUILD_REFERENCES.out.gtf,
+                    BUILD_REFERENCES.out.arriba_ref_blacklist,
+                    BUILD_REFERENCES.out.arriba_ref_known_fusions,
+                    BUILD_REFERENCES.out.arriba_ref_cytobands,
+                    BUILD_REFERENCES.out.arriba_ref_protein_domains
+                )
+
+                ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
+            }
         }
 
+
         //
         // SUBWORKFLOW: Run STAR alignment and StarFusion
         //

From 1b09f4a531dbd7b16a84efa967b2bdbd6903262e Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 2 Jul 2025 12:54:45 +0000
Subject: [PATCH 564/887] fix: back to process limits for github actions

---
 conf/test_build.config | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index 5fcf4c478..a483ed658 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -50,8 +50,8 @@ params {
 // Limit and standardize resources for github actions and reproducibility
 process {
     resourceLimits = [
-        cpus: 8,
-        memory: '16.GB',
+        cpus: 4,
+        memory: '15.GB',
         time: '1.h'
     ]
 }

From c4bd46c21db7afe1c0a32ea74e48f2ef9307c671 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Jul 2025 16:20:24 +0200
Subject: [PATCH 565/887] update test_stub snap

---
 tests/test_stub.nf.test.snap | 43 +++++++++++++++++++++++++++++-------
 1 file changed, 35 insertions(+), 8 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index e7f5410a5..502b30024 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -496,8 +496,11 @@
     },
     "stub test fusioninspector": {
         "content": [
-            22,
+            24,
             {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -567,11 +570,16 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -582,6 +590,7 @@
                 "fastqc/test.zip",
                 "fusioninspector",
                 "fusioninspector/test",
+                "fusioninspector/test/FusionInspector.log",
                 "fusioninspector/test/IGV_inputs",
                 "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
                 "fusioninspector/test/IGV_inputs/cytoBand.txt",
@@ -698,7 +707,6 @@
                 "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
                 "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test/test.FusionInspector.log",
                 "fusioninspector/test/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
@@ -860,14 +868,16 @@
                 "star/test.toTranscriptome.out.bam",
                 "star/test.unmapped_1.fastq.gz",
                 "star/test.unmapped_2.fastq.gz",
-                "star/testXd.out.bam"
+                "star/testXd.out.bam",
+                "vcf",
+                "vcf/test_fusion_data.vcf.gz"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-19T14:09:35.887706"
+        "timestamp": "2025-07-02T14:33:17.006992"
     },
     "stub test salmon": {
         "content": [
@@ -1601,14 +1611,20 @@
     },
     "stub test all": {
         "content": [
-            33,
+            36,
             {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.4.0"
+                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -1706,11 +1722,16 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -1746,6 +1767,7 @@
                 "fusioncatcher/test.summary.txt",
                 "fusioninspector",
                 "fusioninspector/test",
+                "fusioninspector/test/FusionInspector.log",
                 "fusioninspector/test/IGV_inputs",
                 "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
                 "fusioninspector/test/IGV_inputs/cytoBand.txt",
@@ -1862,7 +1884,6 @@
                 "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
                 "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test/test.FusionInspector.log",
                 "fusioninspector/test/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
@@ -1877,6 +1898,10 @@
                 "get",
                 "get/rrna.bed",
                 "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
                 "picard",
                 "picard/test.bai",
                 "picard/test.bam",
@@ -2062,13 +2087,15 @@
                 "stringtie/test.ballgown",
                 "stringtie/test.coverage.gtf",
                 "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
+                "stringtie/test.transcripts.gtf",
+                "vcf",
+                "vcf/test_fusion_data.vcf.gz"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-19T15:22:04.26107"
+        "timestamp": "2025-07-02T14:19:12.460594"
     }
 }
\ No newline at end of file

From fcb7f2d1843850a5ce79ec2a539fda2640e7c9da Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Jul 2025 16:56:42 +0200
Subject: [PATCH 566/887] Update docs/output.md

Co-authored-by: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
---
 docs/output.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index dbccd397f..aec6e6e03 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -149,7 +149,7 @@ If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will f
 
 ### Fastp for fusioncatcher
 
-If `trim_tail_fusioncatcher` is present, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This allows for additional trimming of read tails before running FusionCatcher. For example if reads are 150bp, using --trim_tail_fusioncatcher 50 will shorten reads to 100 bp by 50 bases from the 3′ end. 100 bp is the recommended read length to feed into FusionCatcher.
+If `trim_tail_fusioncatcher` has any value other than 0, [fastp](https://github.com/OpenGene/fastp) will be run again as above. This allows for additional trimming of read tails before running FusionCatcher. For example if reads are 150bp, using `--trim_tail_fusioncatcher 50` will shorten reads to 100 bp by 50 bases from the 3′ end. 100 bp is the recommended read length to feed into FusionCatcher. The default for `--trim_tail_fusioncatcher` is 0 (no trimming).
 
 <details markdown="1">
 <summary>Output files</summary>

From a09b8a6e8806a1ea276abbd4d62c46c3a75c501f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 2 Jul 2025 17:15:18 +0200
Subject: [PATCH 567/887] update test_stub_bam snap

---
 tests/test_stub_bam.nf.test.snap | 522 ++++++++++++++++---------------
 1 file changed, 272 insertions(+), 250 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 88115a4b6..f0bde115d 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -297,10 +297,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:28:43.569555821"
+        "timestamp": "2025-07-02T17:05:41.044302"
     },
     "stub test arriba": {
         "content": [
@@ -418,8 +418,11 @@
     },
     "stub test fusioninspector": {
         "content": [
-            21,
+            23,
             {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -481,11 +484,16 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -495,124 +503,125 @@
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "fusioninspector",
-                "fusioninspector/IGV_inputs",
-                "fusioninspector/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/IGV_inputs/cytoBand.txt",
-                "fusioninspector/IGV_inputs/test.ROI.bed",
-                "fusioninspector/IGV_inputs/test.bed",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/IGV_inputs/test.fa",
-                "fusioninspector/IGV_inputs/test.fa.fai",
-                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/IGV_inputs/test.gtf",
-                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/IGV_inputs/tracks.json",
-                "fusioninspector/chckpts_dir",
-                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/chckpts_dir/blast_filter.ok",
-                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/chckpts_dir/cp_final.ok",
-                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/chckpts_dir/cytoband.ok",
-                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/chckpts_dir/final.abridged.ok",
-                "fusioninspector/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/chckpts_dir/microH.dat.ok",
-                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/chckpts_dir/run_STAR.ok",
-                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/fi_workdir",
-                "fusioninspector/fi_workdir/Log.final.out",
-                "fusioninspector/fi_workdir/Log.out",
-                "fusioninspector/fi_workdir/Log.progress.out",
-                "fusioninspector/fi_workdir/SJ.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome",
-                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/fi_workdir/microH.dat",
-                "fusioninspector/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/fi_workdir/star_align.ok",
-                "fusioninspector/fi_workdir/test.fa",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/fi_workdir/test.gtf",
-                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.fusion_inspector_web.html",
+                "fusioninspector/test",
+                "fusioninspector/test/FusionInspector.log",
+                "fusioninspector/test/IGV_inputs",
+                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/test/IGV_inputs/cytoBand.txt",
+                "fusioninspector/test/IGV_inputs/test.ROI.bed",
+                "fusioninspector/test/IGV_inputs/test.bed",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.fa",
+                "fusioninspector/test/IGV_inputs/test.fa.fai",
+                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/test/IGV_inputs/test.gtf",
+                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/tracks.json",
+                "fusioninspector/test/chckpts_dir",
+                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/test/chckpts_dir/blast_filter.ok",
+                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/test/chckpts_dir/cp_final.ok",
+                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/test/chckpts_dir/cytoband.ok",
+                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/test/chckpts_dir/final.abridged.ok",
+                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/test/chckpts_dir/microH.dat.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/test/chckpts_dir/run_STAR.ok",
+                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/test/fi_workdir",
+                "fusioninspector/test/fi_workdir/Log.final.out",
+                "fusioninspector/test/fi_workdir/Log.out",
+                "fusioninspector/test/fi_workdir/Log.progress.out",
+                "fusioninspector/test/fi_workdir/SJ.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/test/fi_workdir/microH.dat",
+                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/test/fi_workdir/star_align.ok",
+                "fusioninspector/test/fi_workdir/test.fa",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/test/fi_workdir/test.gtf",
+                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -757,14 +766,16 @@
                 "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai"
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "vcf",
+                "vcf/test_fusion_data.vcf.gz"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:30:43.376109569"
+        "timestamp": "2025-07-02T17:14:35.061729"
     },
     "stub test salmon": {
         "content": [
@@ -1504,8 +1515,11 @@
     },
     "stub test all": {
         "content": [
-            33,
+            35,
             {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
                 },
@@ -1604,11 +1618,16 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
@@ -1643,124 +1662,125 @@
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
                 "fusioninspector",
-                "fusioninspector/IGV_inputs",
-                "fusioninspector/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/IGV_inputs/cytoBand.txt",
-                "fusioninspector/IGV_inputs/test.ROI.bed",
-                "fusioninspector/IGV_inputs/test.bed",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/IGV_inputs/test.fa",
-                "fusioninspector/IGV_inputs/test.fa.fai",
-                "fusioninspector/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/IGV_inputs/test.gtf",
-                "fusioninspector/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/IGV_inputs/tracks.json",
-                "fusioninspector/chckpts_dir",
-                "fusioninspector/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/chckpts_dir/blast_filter.ok",
-                "fusioninspector/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/chckpts_dir/cp_final.ok",
-                "fusioninspector/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/chckpts_dir/cytoband.ok",
-                "fusioninspector/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/chckpts_dir/final.abridged.ok",
-                "fusioninspector/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/chckpts_dir/microH.dat.ok",
-                "fusioninspector/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/chckpts_dir/run_STAR.ok",
-                "fusioninspector/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/fi_workdir",
-                "fusioninspector/fi_workdir/Log.final.out",
-                "fusioninspector/fi_workdir/Log.out",
-                "fusioninspector/fi_workdir/Log.progress.out",
-                "fusioninspector/fi_workdir/SJ.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome",
-                "fusioninspector/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/fi_workdir/microH.dat",
-                "fusioninspector/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/fi_workdir/star_align.ok",
-                "fusioninspector/fi_workdir/test.fa",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/fi_workdir/test.gtf",
-                "fusioninspector/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test.FusionInspector.log",
-                "fusioninspector/test.fusion_inspector_web.html",
+                "fusioninspector/test",
+                "fusioninspector/test/FusionInspector.log",
+                "fusioninspector/test/IGV_inputs",
+                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/test/IGV_inputs/cytoBand.txt",
+                "fusioninspector/test/IGV_inputs/test.ROI.bed",
+                "fusioninspector/test/IGV_inputs/test.bed",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.fa",
+                "fusioninspector/test/IGV_inputs/test.fa.fai",
+                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/test/IGV_inputs/test.gtf",
+                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/tracks.json",
+                "fusioninspector/test/chckpts_dir",
+                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/test/chckpts_dir/blast_filter.ok",
+                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/test/chckpts_dir/cp_final.ok",
+                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/test/chckpts_dir/cytoband.ok",
+                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/test/chckpts_dir/final.abridged.ok",
+                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/test/chckpts_dir/microH.dat.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/test/chckpts_dir/run_STAR.ok",
+                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/test/fi_workdir",
+                "fusioninspector/test/fi_workdir/Log.final.out",
+                "fusioninspector/test/fi_workdir/Log.out",
+                "fusioninspector/test/fi_workdir/Log.progress.out",
+                "fusioninspector/test/fi_workdir/SJ.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/test/fi_workdir/microH.dat",
+                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/test/fi_workdir/star_align.ok",
+                "fusioninspector/test/fi_workdir/test.fa",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/test/fi_workdir/test.gtf",
+                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test/test.fusion_inspector_web.html",
                 "fusionreport",
                 "fusionreport/test",
                 "fusionreport/test/AAA_BBB.html",
@@ -1947,13 +1967,15 @@
                 "stringtie/test.ballgown",
                 "stringtie/test.coverage.gtf",
                 "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf"
+                "stringtie/test.transcripts.gtf",
+                "vcf",
+                "vcf/test_fusion_data.vcf.gz"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:26:09.703953189"
+        "timestamp": "2025-07-02T16:55:37.86841"
     }
-}
+}
\ No newline at end of file

From 55cdff291bfe2360a53fe1447cd6c390d408ff92 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 3 Jul 2025 08:18:15 +0200
Subject: [PATCH 568/887] Update workflows/rnafusion.nf

Co-authored-by: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 24799b728..312222adb 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -255,7 +255,7 @@ workflow RNAFUSION {
         def ch_fusioncatcher_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set fusioncatcher fusions to empty by default
         if(tools.contains("fusioncatcher")) {
             fusions_created = true
-        fusioncatcher_trimming = { params.trim_tail_fusioncatcher != 0 }
+        fusioncatcher_trimming = params.trim_tail_fusioncatcher != 0
         FUSIONCATCHER_WORKFLOW (
             ch_reads,
             fusioncatcher_trimming,

From 997e3e1fb2138ae1d4f8050529d441bbb84d7c25 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 3 Jul 2025 10:47:27 +0200
Subject: [PATCH 569/887] update snap

---
 tests/test_stub_bam.nf.test.snap | 73 ++++++++++----------------------
 1 file changed, 23 insertions(+), 50 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index bcb2720bb..a4661d9f1 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -181,10 +181,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:56:57.989800452"
+        "timestamp": "2025-07-03T10:14:43.131367"
     },
     "stub test stringtie": {
         "content": [
@@ -297,10 +297,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:28:43.569555821"
+        "timestamp": "2025-07-03T10:28:11.69711"
     },
     "stub test arriba": {
         "content": [
@@ -762,9 +762,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T13:32:16.607819"
+        "timestamp": "2025-07-03T10:36:59.030953"
     },
     "stub test salmon": {
         "content": [
@@ -871,10 +871,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:30:08.850942124"
+        "timestamp": "2025-07-03T10:34:40.653967"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -1077,14 +1077,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:27:17.26404911"
+        "timestamp": "2025-07-03T09:40:30.61019"
     },
     "stub test fusionreport": {
         "content": [
-            21,
+            20,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1092,9 +1092,6 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
-                "FASTP_FOR_FUSIONCATCHER": {
-                    "fastp": "0.24.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1159,12 +1156,6 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "fastp_for_fusioncatcher",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1322,9 +1313,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T13:32:03.861817"
+        "timestamp": "2025-07-03T10:30:53.270435"
     },
     "stub test fastp": {
         "content": [
@@ -1421,11 +1412,8 @@
     },
     "stub test fusioncatcher": {
         "content": [
-            14,
+            13,
             {
-                "FASTP_FOR_FUSIONCATCHER": {
-                    "fastp": "0.24.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1469,12 +1457,6 @@
                 }
             },
             [
-                "fastp_for_fusioncatcher",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1516,13 +1498,13 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T13:03:53.381632"
+        "timestamp": "2025-07-03T09:42:24.513478"
     },
     "stub test all": {
         "content": [
-            34,
+            33,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1542,9 +1524,6 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
-                "FASTP_FOR_FUSIONCATCHER": {
-                    "fastp": "0.24.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1653,12 +1632,6 @@
                 "fastp/test.fastp.log",
                 "fastp/test_1.fastp.fastq.gz",
                 "fastp/test_2.fastp.fastq.gz",
-                "fastp_for_fusioncatcher",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1979,8 +1952,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T12:58:38.773344"
+        "timestamp": "2025-07-03T08:25:21.978273"
     }
 }
\ No newline at end of file

From 7243df2e25bf30da80124a22cee1cbf69040230b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 3 Jul 2025 11:33:42 +0200
Subject: [PATCH 570/887] update snap

---
 tests/test_stub.nf.test.snap | 45 ++++++++----------------------------
 1 file changed, 9 insertions(+), 36 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 39a65ed93..48a62fe0f 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1191,7 +1191,7 @@
     },
     "stub test fusionreport": {
         "content": [
-            23,
+            22,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1199,9 +1199,6 @@
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.4.0"
                 },
-                "FASTP_FOR_FUSIONCATCHER": {
-                    "fastp": "0.24.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1274,12 +1271,6 @@
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "fastp_for_fusioncatcher",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1456,9 +1447,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T11:14:43.400962"
+        "timestamp": "2025-07-03T11:12:10.068978"
     },
     "stub test fastp": {
         "content": [
@@ -1536,11 +1527,8 @@
     },
     "stub test fusioncatcher": {
         "content": [
-            11,
+            10,
             {
-                "FASTP_FOR_FUSIONCATCHER": {
-                    "fastp": "0.24.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1574,12 +1562,6 @@
                 }
             },
             [
-                "fastp_for_fusioncatcher",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1612,9 +1594,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T11:08:43.632179"
+        "timestamp": "2025-07-03T11:04:33.615515"
     },
     "stub test fusioncatcher trim": {
         "content": [
@@ -1700,7 +1682,7 @@
     },
     "stub test all": {
         "content": [
-            35,
+            34,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.4.0"
@@ -1720,9 +1702,6 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
-                "FASTP_FOR_FUSIONCATCHER": {
-                    "fastp": "0.24.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1839,12 +1818,6 @@
                 "fastp/test.fastp.log",
                 "fastp/test_1.fastp.fastq.gz",
                 "fastp/test_2.fastp.fastq.gz",
-                "fastp_for_fusioncatcher",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.log",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_1.fastp.fastq.gz",
-                "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher_2.fastp.fastq.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -2181,8 +2154,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T11:24:03.086146"
+        "timestamp": "2025-07-03T10:53:04.22172"
     }
 }
\ No newline at end of file

From 3bf196c827d6a335bd9ac6e9fb49f8ac09918e9a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 3 Jul 2025 14:28:28 +0200
Subject: [PATCH 571/887] update snap

---
 tests/test_stub_bam.nf.test.snap | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index a4661d9f1..ba7b85d33 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -1315,7 +1315,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T10:30:53.270435"
+        "timestamp": "2025-07-03T14:17:58.683212"
     },
     "stub test fastp": {
         "content": [

From b2acc2270fee41bbd0ebe29e147f387989625e3d Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 3 Jul 2025 14:43:35 +0000
Subject: [PATCH 572/887] docs: add missing pr number

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3277e3f06..70ad965d6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -104,7 +104,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed test-build as reference building gets integrated in the main workflow [#505](https://github.com/nf-core/rnafusion/pull/505)
 - Removed parameter `--build_references`
 - Removed fusioncatcher build [#650](https://github.com/nf-core/rnafusion/pull/650)
-- Removed subworkflow with less than two modules: `ARRIBA_WORKFLOW` [#xxx](https://github.com/nf-core/rnafusion/pull/xxx)
+- Removed subworkflow with less than two modules: `ARRIBA_WORKFLOW` [#692](https://github.com/nf-core/rnafusion/pull/692)
 
 ### Parameters
 

From 5773aeb6667ce09673e6263d94153124c221168c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Jul 2025 09:35:50 +0200
Subject: [PATCH 573/887] increase memory

---
 conf/modules.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/modules.config b/conf/modules.config
index cf8bd8bb7..d5827c2d2 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -299,7 +299,7 @@ process {
     withName: 'STARFUSION_BUILD' {
         ext.args = { params.species.contains("homo_sapiens") ? "--human_gencode_filter": '' }
         cpus   = { 24    * task.attempt }
-        memory = { 100.GB * task.attempt }
+        memory = { 150.GB * task.attempt }
         time   = { 2.d  * task.attempt }
         publishDir = [
             path: { "${params.genomes_base}/starfusion" },

From 7ea3cc390ddfff31b81b821d33ddae146b9f5efa Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Jul 2025 09:39:22 +0200
Subject: [PATCH 574/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 94c5a7d6b..b6cd3a278 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -97,6 +97,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - The `FUSIONINSPECTOR` process will no longer fail when no fusions have been found. [#651](https://github.com/nf-core/rnafusion/pull/651)
 - Fixed STAR-Fusion build would fail when downloading Pfam and Dfam resources behind SSL. [#653](https://github.com/nf-core/rnafusion/pull/653)
 - Fix bug in argument handling for FusionInspector [#669](https://github.com/nf-core/rnafusion/pull/669)
+- Upgrade STAR-Fusion to 1.15.1 to solve problem building STAR-Fusion references with `--human_gencode_filter` [#683](https://github.com/nf-core/rnafusion/pull/683)
 
 ### Removed
 

From 9741a8e1167a0bd9d366538394fb60275498a95b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Jul 2025 09:44:40 +0200
Subject: [PATCH 575/887] update conda env

---
 modules/local/starfusion/build/environment.yml  | 2 +-
 modules/local/starfusion/detect/environment.yml | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/build/environment.yml b/modules/local/starfusion/build/environment.yml
index 67f00c3c0..f62e01ff4 100644
--- a/modules/local/starfusion/build/environment.yml
+++ b/modules/local/starfusion/build/environment.yml
@@ -5,4 +5,4 @@ dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
   - bioconda::minimap2=2.30
-  - bioconda::star-fusion=1.15.0
+  - bioconda::star-fusion=1.15.1
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/local/starfusion/detect/environment.yml
index 67f00c3c0..f62e01ff4 100644
--- a/modules/local/starfusion/detect/environment.yml
+++ b/modules/local/starfusion/detect/environment.yml
@@ -5,4 +5,4 @@ dependencies:
   - bioconda::dfam=3.7
   - bioconda::hmmer=3.4
   - bioconda::minimap2=2.30
-  - bioconda::star-fusion=1.15.0
+  - bioconda::star-fusion=1.15.1

From b29114c45a19af53e20c4b09865ed15b38957c9c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Jul 2025 13:41:07 +0200
Subject: [PATCH 576/887] update arriba

---
 modules.json                                  |  4 +-
 modules/nf-core/arriba/arriba/environment.yml |  4 +-
 modules/nf-core/arriba/arriba/main.nf         | 31 +++++-----
 .../nf-core/arriba/arriba/tests/main.nf.test  | 56 +------------------
 .../arriba/arriba/tests/main.nf.test.snap     | 53 ++++++++++++++++++
 .../nf-core/arriba/download/environment.yml   |  4 +-
 modules/nf-core/arriba/download/main.nf       | 34 +++++------
 .../arriba/download/tests/main.nf.test.snap   | 24 ++++----
 8 files changed, 107 insertions(+), 103 deletions(-)

diff --git a/modules.json b/modules.json
index b7ce82d85..cb9aa0267 100644
--- a/modules.json
+++ b/modules.json
@@ -12,12 +12,12 @@
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "1719e20e3881b37b9e1e8498ea669a2b92e54b9a",
+                        "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
                         "installed_by": ["modules"]
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
                         "installed_by": ["modules"]
                     },
                     "cat/cat": {
diff --git a/modules/nf-core/arriba/arriba/environment.yml b/modules/nf-core/arriba/arriba/environment.yml
index c3a88ffbd..b30731312 100644
--- a/modules/nf-core/arriba/arriba/environment.yml
+++ b/modules/nf-core/arriba/arriba/environment.yml
@@ -3,6 +3,6 @@
 channels:
   - conda-forge
   - bioconda
-
 dependencies:
-  - bioconda::arriba=2.4.0
+  - bioconda::arriba=2.5.0
+  - conda-forge::wget=1.21.4
diff --git a/modules/nf-core/arriba/arriba/main.nf b/modules/nf-core/arriba/arriba/main.nf
index b89231280..bb4b63770 100644
--- a/modules/nf-core/arriba/arriba/main.nf
+++ b/modules/nf-core/arriba/arriba/main.nf
@@ -4,8 +4,8 @@ process ARRIBA_ARRIBA {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
-        'biocontainers/arriba:2.4.0--h0033a41_2' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/27/27475cdcdbcc8c0ffb6b5ca8c2e6567dbe490edb96f5df4e8f01f4f95912dcd3/data' :
+        'community.wave.seqera.io/library/arriba_wget:a3e48cf793a0b654' }"
 
     input:
     tuple val(meta),  path(bam)
@@ -25,25 +25,26 @@ process ARRIBA_ARRIBA {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
+    def args   = task.ext.args   ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def blacklist_arg = blacklist ? "-b $blacklist" : "-f blacklist"
-    def known_fusions_arg = known_fusions ? "-k $known_fusions" : ""
-    def cytobands_arg = cytobands ? "-d $cytobands" : ""
-    def protein_domains_arg = protein_domains ? "-p $protein_domains" : ""
+
+    def blacklist_arg       = blacklist       ? "-b ${blacklist}"       : "-f blacklist"
+    def known_fusions_arg   = known_fusions   ? "-k ${known_fusions}"   : ""
+    def cytobands_arg       = cytobands       ? "-d ${cytobands}"       : ""
+    def protein_domains_arg = protein_domains ? "-p ${protein_domains}" : ""
 
     """
     arriba \\
-        -x $bam \\
-        -a $fasta \\
-        -g $gtf \\
+        -x ${bam} \\
+        -a ${fasta} \\
+        -g ${gtf} \\
         -o ${prefix}.fusions.tsv \\
         -O ${prefix}.fusions.discarded.tsv \\
-        $blacklist_arg \\
-        $known_fusions_arg \\
-        $cytobands_arg \\
-        $protein_domains_arg \\
-        $args
+        ${blacklist_arg} \\
+        ${known_fusions_arg} \\
+        ${cytobands_arg} \\
+        ${protein_domains_arg} \\
+        ${args}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test b/modules/nf-core/arriba/arriba/tests/main.nf.test
index 4cff86e50..e76c06871 100644
--- a/modules/nf-core/arriba/arriba/tests/main.nf.test
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test
@@ -13,59 +13,7 @@ nextflow_process {
     tag "star/genomegenerate"
     tag "star/align"
 
-    setup {
-        config "./nextflow.config"
-        options "-stub"
-        run("ARRIBA_DOWNLOAD") {
-            script "../../../arriba/download/main.nf"
-            process {
-                """
-                input[0] = 'GRCh38'
-                """
-            }
-        }
-        run("STAR_GENOMEGENERATE") {
-            script "../../../star/genomegenerate/main.nf"
-            options "-stub"
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test_fasta' ],
-                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
-                ])
-                input[1] = Channel.of([
-                    [ id:'test_gtf' ],
-                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
-                ])
-                """
-            }
-        }
-        run("STAR_ALIGN") {
-            script "../../../star/align/main.nf"
-            options "-stub"
-            process {
-                """
-                input[0] = Channel.of([
-                    [ id:'test', single_end:false ], // meta map
-                    [
-                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
-                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
-                    ]
-                ])
-                input[1] = STAR_GENOMEGENERATE.out.index
-                input[2] = Channel.of([
-                    [ id:'test_gtf' ],
-                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
-                ])
-                input[3] = false
-                input[4] = 'illumina'
-                input[5] = false
-                """
-            }
-        }
-    }
-
-    test("homo_sapiens - paired_end - stub") {
+    test("arriba - homo_sapiens - paired_end - stub") {
 
         options "-stub"
 
@@ -101,7 +49,5 @@ nextflow_process {
                 { assert snapshot(process.out).match() }
             )
         }
-
     }
-
 }
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test.snap b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
index 14d46f6b8..7cf286a2f 100644
--- a/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
@@ -1,4 +1,57 @@
 {
+    "arriba - homo_sapiens - paired_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,d2eb9bb3bd4b21571a6aa2f39f1283af"
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "fusions_fail": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,d2eb9bb3bd4b21571a6aa2f39f1283af"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-04T12:35:18.703566"
+    },
     "homo_sapiens - paired_end - stub": {
         "content": [
             {
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
index e8f5b278f..5c2402759 100644
--- a/modules/nf-core/arriba/download/environment.yml
+++ b/modules/nf-core/arriba/download/environment.yml
@@ -4,4 +4,6 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::arriba=2.4.0
+  - bioconda::arriba=2.5.0
+  - conda-forge::wget=1.21.4
+
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index c45120adb..fdd46d2e8 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -4,29 +4,30 @@ process ARRIBA_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
-        'biocontainers/arriba:2.4.0--h0033a41_2' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/27/27475cdcdbcc8c0ffb6b5ca8c2e6567dbe490edb96f5df4e8f01f4f95912dcd3/data' :
+        'community.wave.seqera.io/library/arriba_wget:a3e48cf793a0b654' }"
 
     input:
     val(genome)
 
     output:
-    path "blacklist*${genome}*.tsv.gz"       , emit: blacklist
-    path "cytobands*${genome}*.tsv"          , emit: cytobands
-    path "protein_domains*${genome}*.gff3"   , emit: protein_domains
-    path "known_fusions*${genome}*.tsv.gz"   , emit: known_fusions
-    path "versions.yml"                      , emit: versions
+    path "blacklist*${genome}*.tsv.gz"    , emit: blacklist
+    path "cytobands*${genome}*.tsv"       , emit: cytobands
+    path "protein_domains*${genome}*.gff3", emit: protein_domains
+    path "known_fusions*${genome}*.tsv.gz", emit: known_fusions
+    path "versions.yml"                   , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
+    def arriba_version = '2.5.0'
     """
-    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz --no-check-certificate
-    tar -xzvf arriba_v2.4.0.tar.gz
-    rm arriba_v2.4.0.tar.gz
-    mv arriba_v2.4.0/database/* .
-    rm -r arriba_v2.4.0
+    wget https://github.com/suhrig/arriba/releases/download/v${arriba_version}/arriba_v${arriba_version}.tar.gz -O arriba_v${arriba_version}.tar.gz --no-check-certificate
+    tar -xzvf arriba_v${arriba_version}.tar.gz
+    rm arriba_v${arriba_version}.tar.gz
+    mv arriba_v${arriba_version}/database/* .
+    rm -r arriba_v${arriba_version}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -35,11 +36,12 @@ process ARRIBA_DOWNLOAD {
     """
 
     stub:
+    def arriba_version = '2.5.0'
     """
-    touch blacklist_hg38_GRCh38_v2.4.0.tsv.gz
-    touch protein_domains_hg38_GRCh38_v2.4.0.gff3
-    touch cytobands_hg38_GRCh38_v2.4.0.tsv
-    touch known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
+    touch blacklist_hg38_GRCh38_v${arriba_version}.tsv.gz
+    touch protein_domains_hg38_GRCh38_v${arriba_version}.gff3
+    touch cytobands_hg38_GRCh38_v${arriba_version}.tsv
+    touch known_fusions_hg38_GRCh38_v${arriba_version}.tsv.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
index d12a38bbf..87e41a84b 100644
--- a/modules/nf-core/arriba/download/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/download/tests/main.nf.test.snap
@@ -3,41 +3,41 @@
         "content": [
             {
                 "0": [
-                    "blacklist_hg38_GRCh38_v2.4.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
+                    "blacklist_hg38_GRCh38_v2.5.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
                 ],
                 "1": [
-                    "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
+                    "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
                 ],
                 "2": [
-                    "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
+                    "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
                 ],
                 "3": [
-                    "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
+                    "known_fusions_hg38_GRCh38_v2.5.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
                 ],
                 "4": [
-                    "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+                    "versions.yml:md5,2457cdc32bc582854616aba806a91d35"
                 ],
                 "blacklist": [
-                    "blacklist_hg38_GRCh38_v2.4.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
+                    "blacklist_hg38_GRCh38_v2.5.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
                 ],
                 "cytobands": [
-                    "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
+                    "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
                 ],
                 "known_fusions": [
-                    "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
+                    "known_fusions_hg38_GRCh38_v2.5.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
                 ],
                 "protein_domains": [
-                    "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
+                    "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
                 ],
                 "versions": [
-                    "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+                    "versions.yml:md5,2457cdc32bc582854616aba806a91d35"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-12-16T01:46:32.110653034"
+        "timestamp": "2025-07-04T12:36:28.499552"
     }
 }
\ No newline at end of file

From 8621cf17cc1c8f5f1dda8ea0e5f5b7b0ac6ea6e5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 4 Jul 2025 14:06:56 +0200
Subject: [PATCH 577/887] update arriba2

---
 modules.json                                    | 2 +-
 modules/nf-core/arriba/download/environment.yml | 1 -
 2 files changed, 1 insertion(+), 2 deletions(-)

diff --git a/modules.json b/modules.json
index cb9aa0267..47d139606 100644
--- a/modules.json
+++ b/modules.json
@@ -17,7 +17,7 @@
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
+                        "git_sha": "75771aaffe150962320e3c8c9a025268843fcc46",
                         "installed_by": ["modules"]
                     },
                     "cat/cat": {
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
index 5c2402759..b30731312 100644
--- a/modules/nf-core/arriba/download/environment.yml
+++ b/modules/nf-core/arriba/download/environment.yml
@@ -6,4 +6,3 @@ channels:
 dependencies:
   - bioconda::arriba=2.5.0
   - conda-forge::wget=1.21.4
-

From d0b54bb4ef03e4876577c190dff89df7a4fc80a7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Jul 2025 10:35:26 +0200
Subject: [PATCH 578/887] update version

---
 .../arriba/visualisation/tests/main.nf.test   |  8 +++----
 nextflow.config                               |  8 +++----
 .../local/arriba_workflow/tests/main.nf.test  | 24 +++++++++----------
 tests/.nftignore                              |  4 ++--
 tests/test_build.nf.test.snap                 |  8 +++----
 5 files changed, 26 insertions(+), 26 deletions(-)

diff --git a/modules/local/arriba/visualisation/tests/main.nf.test b/modules/local/arriba/visualisation/tests/main.nf.test
index 1a18b0db3..bdf5269e0 100644
--- a/modules/local/arriba/visualisation/tests/main.nf.test
+++ b/modules/local/arriba/visualisation/tests/main.nf.test
@@ -25,11 +25,11 @@ nextflow_process {
                 ]
                 input[2] = [
                     [ id:'test_protein_domains' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true)
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true)
                 ]
                 input[3] = [
                     [ id:'test_cytobands' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true)
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true)
                 ]
                 """
             }
@@ -63,11 +63,11 @@ nextflow_process {
                 ]
                 input[2] = [
                     [ id:'test_protein_domains' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true)
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true)
                 ]
                 input[3] = [
                     [ id:'test_cytobands' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true)
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true)
                 ]
                 """
             }
diff --git a/nextflow.config b/nextflow.config
index 9f5536013..ed615a07e 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -73,10 +73,10 @@ params {
     refflat                       = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf.refflat"
     rrna_intervals                = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.interval_list"
     no_cosmic                     = false
-    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz"
-    arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv"
-    arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz"
-    arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3"
+    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+    arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}"
     hgnc_ref                      = "${params.genomes_base}/hgnc/hgnc_complete_set.txt"
     hgnc_date                     = "${params.genomes_base}/hgnc/HGNC-DB-timestamp.txt"
diff --git a/subworkflows/local/arriba_workflow/tests/main.nf.test b/subworkflows/local/arriba_workflow/tests/main.nf.test
index e49a3768c..6080e1436 100644
--- a/subworkflows/local/arriba_workflow/tests/main.nf.test
+++ b/subworkflows/local/arriba_workflow/tests/main.nf.test
@@ -73,28 +73,28 @@ nextflow_workflow {
                 // ch_arriba_ref_blacklist
                 input[4] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_known_fusions
                 input[5] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_cytobands
                 input[6] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_protein_domains
                 input[7] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
@@ -198,28 +198,28 @@ nextflow_workflow {
                 // ch_arriba_ref_blacklist
                 input[4] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_known_fusions
                 input[5] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_cytobands
                 input[6] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_protein_domains
                 input[7] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
@@ -322,28 +322,28 @@ nextflow_workflow {
                 // ch_arriba_ref_blacklist
                 input[4] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_known_fusions
                 input[5] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_cytobands
                 input[6] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
                 // ch_arriba_ref_protein_domains
                 input[7] =
                     Channel.fromPath(
-                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3", checkIfExists: true
+                        "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true
                     )
                     .map{ [ [ id: it.name ], it ] }
 
diff --git a/tests/.nftignore b/tests/.nftignore
index 3bba86da0..c11f3e9d4 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -1,6 +1,6 @@
 pipeline_info/*.{html,json,txt,yml}
-references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
-references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
+references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz
+references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz
 references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa
 references/hgnc/HGNC-DB-timestamp.txt
 references/hgnc/hgnc_complete_set.txt
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 91f926bb9..2dbc57023 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -56,10 +56,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/fusion_report.log",

From 8cec4a8ceec240a3d697670441eba7987fa3aa6d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 8 Jul 2025 12:52:07 +0200
Subject: [PATCH 579/887] update test stub snap

---
 tests/test_stub.nf.test.snap | 62 ++++++++++++++++++------------------
 1 file changed, 31 insertions(+), 31 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 6ea02a74e..324da36e0 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -358,10 +358,10 @@
             16,
             {
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -440,10 +440,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -490,9 +490,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-13T16:44:25.412602"
+        "timestamp": "2025-07-08T11:31:30.841349"
     },
     "stub test fusioninspector": {
         "content": [
@@ -502,10 +502,10 @@
                     "agat": "v1.4.1"
                 },
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.4.0"
@@ -738,10 +738,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/cosmic.db",
@@ -875,9 +875,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T14:33:17.006992"
+        "timestamp": "2025-07-08T11:48:14.166006"
     },
     "stub test salmon": {
         "content": [
@@ -1205,10 +1205,10 @@
             22,
             {
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1319,10 +1319,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/cosmic.db",
@@ -1460,7 +1460,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T11:12:10.068978"
+        "timestamp": "2025-07-08T11:42:59.015299"
     },
     "stub test fastp": {
         "content": [
@@ -1699,10 +1699,10 @@
                     "agat": "v1.4.1"
                 },
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.4.0"
@@ -1997,10 +1997,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/cosmic.db",
@@ -2176,8 +2176,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T14:19:12.460594"
+        "timestamp": "2025-07-08T11:29:35.207225"
     }
 }
\ No newline at end of file

From f387154d6ef04fe2cac5358ce3f5036529d79360 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Tue, 8 Jul 2025 11:39:17 +0000
Subject: [PATCH 580/887] Template update for nf-core/tools version 3.3.2

---
 .github/actions/nf-test/action.yml            |   4 -
 .github/workflows/linting.yml                 |   2 +-
 .github/workflows/linting_comment.yml         |   2 +-
 .github/workflows/nf-test.yml                 |  45 +++----
 .github/workflows/release-announcements.yml   |   2 +-
 .nf-core.yml                                  |   2 +-
 .pre-commit-config.yaml                       |   2 +-
 README.md                                     |   6 +-
 assets/schema_input.json                      |   4 +-
 conf/base.config                              |   1 +
 modules.json                                  |   4 +-
 modules/nf-core/fastqc/environment.yml        |   2 +
 modules/nf-core/fastqc/main.nf                |   2 +-
 modules/nf-core/fastqc/meta.yml               |  23 ++--
 modules/nf-core/multiqc/environment.yml       |   4 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 modules/nf-core/multiqc/meta.yml              | 110 ++++++++++--------
 .../nf-core/multiqc/tests/main.nf.test.snap   |  18 +--
 nextflow.config                               |   5 +-
 nf-test.config                                |   2 +-
 ro-crate-metadata.json                        |  40 +++----
 .../tests/nextflow.config                     |   2 +-
 tests/.nftignore                              |   1 +
 tests/nextflow.config                         |   6 +-
 24 files changed, 158 insertions(+), 135 deletions(-)

diff --git a/.github/actions/nf-test/action.yml b/.github/actions/nf-test/action.yml
index 243e78238..bf44d9612 100644
--- a/.github/actions/nf-test/action.yml
+++ b/.github/actions/nf-test/action.yml
@@ -54,13 +54,9 @@ runs:
         conda-solver: libmamba
         conda-remove-defaults: true
 
-      # TODO Skip failing conda tests and document their failures
-      # https://github.com/nf-core/modules/issues/7017
     - name: Run nf-test
       shell: bash
       env:
-        NFT_DIFF: ${{ env.NFT_DIFF }}
-        NFT_DIFF_ARGS: ${{ env.NFT_DIFF_ARGS }}
         NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
       run: |
         nf-test test \
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index f2d7d1dd7..8b0f88c36 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -13,7 +13,7 @@ jobs:
     steps:
       - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
-      - name: Set up Python 3.12
+      - name: Set up Python 3.13
         uses: actions/setup-python@a26af69be951a213d495a4c3e4e4022e16d87065 # v5
         with:
           python-version: "3.13"
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index 7e8050fb8..d43797d9d 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@4c1e823582f43b179e2cbb49c3eade4e41f992e2 # v10
+        uses: dawidd6/action-download-artifact@ac66b43f0e6a346234dd65d4d0c8fbb31cb316e5 # v11
         with:
           workflow: linting.yml
           workflow_conclusion: completed
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index f03aea0c0..e7b58449b 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -1,12 +1,5 @@
 name: Run nf-test
 on:
-  push:
-    paths-ignore:
-      - "docs/**"
-      - "**/meta.yml"
-      - "**/*.md"
-      - "**/*.png"
-      - "**/*.svg"
   pull_request:
     paths-ignore:
       - "docs/**"
@@ -35,7 +28,7 @@ jobs:
   nf-test-changes:
     name: nf-test-changes
     runs-on: # use self-hosted runners
-      - runs-on=$-nf-test-changes
+      - runs-on=${{ github.run_id }}-nf-test-changes
       - runner=4cpu-linux-x64
     outputs:
       shard: ${{ steps.set-shards.outputs.shard }}
@@ -69,7 +62,7 @@ jobs:
     needs: [nf-test-changes]
     if: ${{ needs.nf-test-changes.outputs.total_shards != '0' }}
     runs-on: # use self-hosted runners
-      - runs-on=$-nf-test
+      - runs-on=${{ github.run_id }}-nf-test
       - runner=4cpu-linux-x64
     strategy:
       fail-fast: false
@@ -85,7 +78,7 @@ jobs:
           - isMain: false
             profile: "singularity"
         NXF_VER:
-          - "24.04.2"
+          - "24.10.5"
           - "latest-everything"
     env:
       NXF_ANSI_LOG: false
@@ -97,23 +90,39 @@ jobs:
           fetch-depth: 0
 
       - name: Run nf-test
+        id: run_nf_test
         uses: ./.github/actions/nf-test
+        continue-on-error: ${{ matrix.NXF_VER == 'latest-everything' }}
         env:
-          NFT_DIFF: ${{ env.NFT_DIFF }}
-          NFT_DIFF_ARGS: ${{ env.NFT_DIFF_ARGS }}
           NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
         with:
           profile: ${{ matrix.profile }}
           shard: ${{ matrix.shard }}
           total_shards: ${{ env.TOTAL_SHARDS }}
+
+      - name: Report test status
+        if: ${{ always() }}
+        run: |
+          if [[ "${{ steps.run_nf_test.outcome }}" == "failure" ]]; then
+            echo "::error::Test with ${{ matrix.NXF_VER }} failed"
+            # Add to workflow summary
+            echo "## ❌ Test failed: ${{ matrix.profile }} | ${{ matrix.NXF_VER }} | Shard ${{ matrix.shard }}/${{ env.TOTAL_SHARDS }}" >> $GITHUB_STEP_SUMMARY
+            if [[ "${{ matrix.NXF_VER }}" == "latest-everything" ]]; then
+              echo "::warning::Test with latest-everything failed but will not cause workflow failure. Please check if the error is expected or if it needs fixing."
+            fi
+            if [[ "${{ matrix.NXF_VER }}" != "latest-everything" ]]; then
+              exit 1
+            fi
+          fi
+
   confirm-pass:
     needs: [nf-test]
     if: always()
     runs-on: # use self-hosted runners
-      - runs-on=$-confirm-pass
+      - runs-on=${{ github.run_id }}-confirm-pass
       - runner=2cpu-linux-x64
     steps:
-      - name: One or more tests failed
+      - name: One or more tests failed (excluding latest-everything)
         if: ${{ contains(needs.*.result, 'failure') }}
         run: exit 1
 
@@ -132,11 +141,3 @@ jobs:
           echo "DEBUG: toJSON(needs) = ${{ toJSON(needs) }}"
           echo "DEBUG: toJSON(needs.*.result) = ${{ toJSON(needs.*.result) }}"
           echo "::endgroup::"
-
-      - name: Clean Workspace # Purge the workspace in case it's running on a self-hosted runner
-        if: always()
-        run: |
-          ls -la ./
-          rm -rf ./* || true
-          rm -rf ./.??* || true
-          ls -la ./
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 4abaf4843..0f7324956 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -30,7 +30,7 @@ jobs:
   bsky-post:
     runs-on: ubuntu-latest
     steps:
-      - uses: zentered/bluesky-post-action@4aa83560bb3eac05dbad1e5f221ee339118abdd2 # v0.2.0
+      - uses: zentered/bluesky-post-action@6461056ea355ea43b977e149f7bf76aaa572e5e8 # v0.3.0
         with:
           post: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
diff --git a/.nf-core.yml b/.nf-core.yml
index 3eb57693e..9b7277aa0 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,7 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
-nf_core_version: 3.3.1
+nf_core_version: 3.3.2
 repository_type: pipeline
 template:
   author: Martin Proks, Annick Renevey
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 9d0b248d3..bb41beec1 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -4,7 +4,7 @@ repos:
     hooks:
       - id: prettier
         additional_dependencies:
-          - prettier@3.5.0
+          - prettier@3.6.2
   - repo: https://github.com/pre-commit/pre-commit-hooks
     rev: v5.0.0
     hooks:
diff --git a/README.md b/README.md
index 1bd640313..9d6bbafe4 100644
--- a/README.md
+++ b/README.md
@@ -5,12 +5,12 @@
   </picture>
 </h1>
 
-[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)
+[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)
 [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
-[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.04.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
-[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.1)
+[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
+[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
diff --git a/assets/schema_input.json b/assets/schema_input.json
index 4f1f739a0..f063db7c1 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -17,14 +17,14 @@
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "pattern": "^([\\S\\s]*\\/)?[^\\s\\/]+\\.f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
+                "pattern": "^([\\S\\s]*\\/)?[^\\s\\/]+\\.f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             }
         },
diff --git a/conf/base.config b/conf/base.config
index e8c3525d6..0cbda3c71 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -61,5 +61,6 @@ process {
     }
     withLabel: process_gpu {
         ext.use_gpu = { workflow.profile.contains('gpu') }
+        accelerator = { workflow.profile.contains('gpu') ? 1 : null }
     }
 }
diff --git a/modules.json b/modules.json
index 296d35429..9456129af 100644
--- a/modules.json
+++ b/modules.json
@@ -7,12 +7,12 @@
                 "nf-core": {
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
index 691d4c763..f9f54ee9b 100644
--- a/modules/nf-core/fastqc/environment.yml
+++ b/modules/nf-core/fastqc/environment.yml
@@ -1,3 +1,5 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 033f4154a..23e16634c 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -29,7 +29,7 @@ process FASTQC {
     // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
     // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
     // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
-    def memory_in_mb = task.memory ? task.memory.toUnit('MB').toFloat() / task.cpus : null
+    def memory_in_mb = task.memory ? task.memory.toUnit('MB') / task.cpus : null
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
 
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index 2b2e62b8a..c8d9d025a 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -29,9 +29,10 @@ input:
         description: |
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively.
+        ontologies: []
 output:
-  - html:
-      - meta:
+  html:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -40,8 +41,9 @@ output:
           type: file
           description: FastQC report
           pattern: "*_{fastqc.html}"
-  - zip:
-      - meta:
+          ontologies: []
+  zip:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -50,11 +52,14 @@ output:
           type: file
           description: FastQC report archive
           pattern: "*_{fastqc.zip}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@drpatelh"
   - "@grst"
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index a27122ce1..812fc4c5e 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -1,5 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.27
+  - bioconda::multiqc=1.29
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 58d9313c6..0ac3c3699 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.27--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.27--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.29--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.29--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index b16c18792..ce30eb732 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -15,57 +15,71 @@ tools:
       licence: ["GPL-3.0-or-later"]
       identifier: biotools:multiqc
 input:
-  - - multiqc_files:
-        type: file
-        description: |
-          List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-  - - multiqc_config:
-        type: file
-        description: Optional config yml for MultiQC
-        pattern: "*.{yml,yaml}"
-  - - extra_multiqc_config:
-        type: file
-        description: Second optional config yml for MultiQC. Will override common sections
-          in multiqc_config.
-        pattern: "*.{yml,yaml}"
-  - - multiqc_logo:
+  - multiqc_files:
+      type: file
+      description: |
+        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+      ontologies: []
+  - multiqc_config:
+      type: file
+      description: Optional config yml for MultiQC
+      pattern: "*.{yml,yaml}"
+      ontologies:
+        - edam: http://edamontology.org/format_3750 # YAML
+  - extra_multiqc_config:
+      type: file
+      description: Second optional config yml for MultiQC. Will override common sections
+        in multiqc_config.
+      pattern: "*.{yml,yaml}"
+      ontologies:
+        - edam: http://edamontology.org/format_3750 # YAML
+  - multiqc_logo:
+      type: file
+      description: Optional logo file for MultiQC
+      pattern: "*.{png}"
+      ontologies: []
+  - replace_names:
+      type: file
+      description: |
+        Optional two-column sample renaming file. First column a set of
+        patterns, second column a set of corresponding replacements. Passed via
+        MultiQC's `--replace-names` option.
+      pattern: "*.{tsv}"
+      ontologies:
+        - edam: http://edamontology.org/format_3475 # TSV
+  - sample_names:
+      type: file
+      description: |
+        Optional TSV file with headers, passed to the MultiQC --sample_names
+        argument.
+      pattern: "*.{tsv}"
+      ontologies:
+        - edam: http://edamontology.org/format_3475 # TSV
+output:
+  report:
+    - "*multiqc_report.html":
         type: file
-        description: Optional logo file for MultiQC
-        pattern: "*.{png}"
-  - - replace_names:
+        description: MultiQC report file
+        pattern: "multiqc_report.html"
+        ontologies: []
+  data:
+    - "*_data":
+        type: directory
+        description: MultiQC data dir
+        pattern: "multiqc_data"
+  plots:
+    - "*_plots":
         type: file
-        description: |
-          Optional two-column sample renaming file. First column a set of
-          patterns, second column a set of corresponding replacements. Passed via
-          MultiQC's `--replace-names` option.
-        pattern: "*.{tsv}"
-  - - sample_names:
+        description: Plots created by MultiQC
+        pattern: "*_data"
+        ontologies: []
+  versions:
+    - versions.yml:
         type: file
-        description: |
-          Optional TSV file with headers, passed to the MultiQC --sample_names
-          argument.
-        pattern: "*.{tsv}"
-output:
-  - report:
-      - "*multiqc_report.html":
-          type: file
-          description: MultiQC report file
-          pattern: "multiqc_report.html"
-  - data:
-      - "*_data":
-          type: directory
-          description: MultiQC data dir
-          pattern: "multiqc_data"
-  - plots:
-      - "*_plots":
-          type: file
-          description: Plots created by MultiQC
-          pattern: "*_data"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@abhi18av"
   - "@bunop"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index 7b7c13220..88e90571c 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,c1fe644a37468f6dae548d98bc72c2c1"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.4"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-01-27T09:29:57.631982377"
+        "timestamp": "2025-05-22T11:50:41.182332996"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,c1fe644a37468f6dae548d98bc72c2c1"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.4"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-01-27T09:30:34.743726958"
+        "timestamp": "2025-05-22T11:51:22.448739369"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f"
+                "versions.yml:md5,c1fe644a37468f6dae548d98bc72c2c1"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.4"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-01-27T09:30:21.44383553"
+        "timestamp": "2025-05-22T11:51:06.198928424"
     }
 }
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index c8fe40eab..cbd3ecc8f 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -235,7 +235,6 @@ dag {
 
 manifest {
     name            = 'nf-core/rnafusion'
-    author          = """Martin Proks, Annick Renevey""" // The author field is deprecated from Nextflow version 24.10.0, use contributors instead
     contributors    = [
         // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0
         [
@@ -259,14 +258,14 @@ manifest {
     description     = """Nextflow rnafusion analysis pipeline, part of the nf-core community."""
     mainScript      = 'main.nf'
     defaultBranch   = 'master'
-    nextflowVersion = '!>=24.04.2'
+    nextflowVersion = '!>=24.10.5'
     version         = '4.0.0dev'
     doi             = ''
 }
 
 // Nextflow plugins
 plugins {
-    id 'nf-schema@2.3.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+    id 'nf-schema@2.4.2' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 
 validation {
diff --git a/nf-test.config b/nf-test.config
index 889df7601..3a1fff59f 100644
--- a/nf-test.config
+++ b/nf-test.config
@@ -9,7 +9,7 @@ config {
     configFile "tests/nextflow.config"
 
     // ignore tests coming from the nf-core/modules repo
-    ignore 'modules/nf-core/**/*', 'subworkflows/nf-core/**/*'
+    ignore 'modules/nf-core/**/tests/*', 'subworkflows/nf-core/**/tests/*'
 
     // run all test with defined profile(s) from the main nextflow.config
     profile "test"
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 21ffe6a16..abbf99167 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,8 +22,8 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
-            "datePublished": "2025-06-03T11:02:37+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.04.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "datePublished": "2025-07-08T11:39:11+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#b677eda3-711d-4250-b4fa-fc2ec307db84"
+                    "@id": "#cfd4e34a-0799-4d73-95ca-6b4c84af3e73"
                 }
             ],
             "name": "nf-core/rnafusion"
@@ -124,20 +124,20 @@
             "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
             "creator": [
                 {
-                    "@id": "https://orcid.org/0000-0001-9017-591X"
+                    "@id": "#phil.ewels@scilifelab.se"
                 },
                 {
-                    "@id": "#phil.ewels@scilifelab.se"
+                    "@id": "#max.u.garcia@gmail.com"
                 },
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
                 },
                 {
-                    "@id": "#max.u.garcia@gmail.com"
+                    "@id": "https://orcid.org/0000-0001-9017-591X"
                 }
             ],
             "dateCreated": "",
-            "dateModified": "2025-06-03T11:02:37Z",
+            "dateModified": "2025-07-08T11:39:11Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
             "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
             "license": ["MIT"],
@@ -166,14 +166,14 @@
             "url": {
                 "@id": "https://www.nextflow.io/"
             },
-            "version": "!>=24.04.2"
+            "version": "!>=24.10.5"
         },
         {
-            "@id": "#b677eda3-711d-4250-b4fa-fc2ec307db84",
+            "@id": "#cfd4e34a-0799-4d73-95ca-6b4c84af3e73",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#d7d8d23f-bc32-4572-bb73-a559c46d3773"
+                    "@id": "#1076262d-a75d-4b99-bdd5-326b8f957f0e"
                 }
             ],
             "mainEntity": {
@@ -182,7 +182,7 @@
             "name": "Test suite for nf-core/rnafusion"
         },
         {
-            "@id": "#d7d8d23f-bc32-4572-bb73-a559c46d3773",
+            "@id": "#1076262d-a75d-4b99-bdd5-326b8f957f0e",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/rnafusion",
             "resource": "repos/nf-core/rnafusion/actions/workflows/nf-test.yml",
@@ -310,18 +310,18 @@
             "name": "nf-core",
             "url": "https://nf-co.re/"
         },
-        {
-            "@id": "https://orcid.org/0000-0001-9017-591X",
-            "@type": "Person",
-            "email": "rickard.hammaren@scilifelab.se",
-            "name": "Rickard Hammar\u00e9n"
-        },
         {
             "@id": "#phil.ewels@scilifelab.se",
             "@type": "Person",
             "email": "phil.ewels@scilifelab.se",
             "name": "Phil Ewels"
         },
+        {
+            "@id": "#max.u.garcia@gmail.com",
+            "@type": "Person",
+            "email": "max.u.garcia@gmail.com",
+            "name": "Maxime Garcia"
+        },
         {
             "@id": "https://orcid.org/0000-0002-8178-3128",
             "@type": "Person",
@@ -329,10 +329,10 @@
             "name": "Martin Proks"
         },
         {
-            "@id": "#max.u.garcia@gmail.com",
+            "@id": "https://orcid.org/0000-0001-9017-591X",
             "@type": "Person",
-            "email": "max.u.garcia@gmail.com",
-            "name": "Maxime Garcia"
+            "email": "rickard.hammaren@scilifelab.se",
+            "name": "Rickard Hammar\u00e9n"
         }
     ]
 }
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
index 0907ac58f..09ef842ae 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
@@ -1,5 +1,5 @@
 plugins {
-    id "nf-schema@2.1.0"
+    id "nf-schema@2.4.2"
 }
 
 validation {
diff --git a/tests/.nftignore b/tests/.nftignore
index c10bc1f11..158c83c59 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -1,5 +1,6 @@
 .DS_Store
 multiqc/multiqc_data/fastqc_top_overrepresented_sequences_table.txt
+multiqc/multiqc_data/BETA-multiqc.parquet
 multiqc/multiqc_data/multiqc.log
 multiqc/multiqc_data/multiqc_data.json
 multiqc/multiqc_data/multiqc_sources.txt
diff --git a/tests/nextflow.config b/tests/nextflow.config
index c00c384c5..ad786e265 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -6,7 +6,9 @@
 
 // TODO nf-core: Specify any additional parameters here
 // Or any resources requirements
-params.modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
-params.pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion'
+params {
+    modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
+    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion'
+}
 
 aws.client.anonymous = true // fixes S3 access issues on self-hosted runners

From 8bf2b989b066f1471659e20a3040ca487f009b4e Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Tue, 8 Jul 2025 14:36:22 +0000
Subject: [PATCH 581/887] Reintegrate fusionreport detect and download from
 nf-core

---
 conf/modules.config                           |   2 +-
 modules.json                                  |  10 ++
 modules/local/fusionreport/detect/meta.yml    |  51 ------
 .../fusionreport/detect/tests/nextflow.config |  10 --
 .../fusionreport/download/environment.yml     |   6 -
 modules/local/fusionreport/download/meta.yml  |  35 ----
 .../fusionreport/detect/environment.yml       |   2 +-
 .../fusionreport/detect/main.nf               |  23 ++-
 modules/nf-core/fusionreport/detect/meta.yml  | 151 ++++++++++++++++++
 .../fusionreport/detect/tests/main.nf.test    |  46 +++---
 .../detect/tests/main.nf.test.snap            |  32 ++--
 .../fusionreport/detect/tests/nextflow.config |  10 ++
 .../fusionreport/download/environment.yml     |  18 +++
 .../fusionreport/download/main.nf             |   7 +-
 .../nf-core/fusionreport/download/meta.yml    |  36 +++++
 .../fusionreport/download/tests/main.nf.test  |  12 +-
 .../download/tests/main.nf.test.snap          |  22 +--
 .../download/tests/nextflow.config            |   2 +-
 subworkflows/local/build_references/main.nf   |   2 +-
 .../local/fusionreport_workflow/main.nf       |  14 +-
 20 files changed, 316 insertions(+), 175 deletions(-)
 delete mode 100644 modules/local/fusionreport/detect/meta.yml
 delete mode 100644 modules/local/fusionreport/detect/tests/nextflow.config
 delete mode 100644 modules/local/fusionreport/download/environment.yml
 delete mode 100644 modules/local/fusionreport/download/meta.yml
 rename modules/{local => nf-core}/fusionreport/detect/environment.yml (71%)
 rename modules/{local => nf-core}/fusionreport/detect/main.nf (67%)
 create mode 100644 modules/nf-core/fusionreport/detect/meta.yml
 rename modules/{local => nf-core}/fusionreport/detect/tests/main.nf.test (64%)
 rename modules/{local => nf-core}/fusionreport/detect/tests/main.nf.test.snap (92%)
 create mode 100644 modules/nf-core/fusionreport/detect/tests/nextflow.config
 create mode 100644 modules/nf-core/fusionreport/download/environment.yml
 rename modules/{local => nf-core}/fusionreport/download/main.nf (80%)
 create mode 100644 modules/nf-core/fusionreport/download/meta.yml
 rename modules/{local => nf-core}/fusionreport/download/tests/main.nf.test (83%)
 rename modules/{local => nf-core}/fusionreport/download/tests/main.nf.test.snap (74%)
 rename modules/{local => nf-core}/fusionreport/download/tests/nextflow.config (55%)

diff --git a/conf/modules.config b/conf/modules.config
index 278ce01a6..95331646b 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -128,7 +128,7 @@ process {
         ]
     }
 
-    withName: 'FUSIONREPORT' {
+    withName: 'FUSIONREPORT_DETECT' {
         ext.args         = { params.no_cosmic ? "--no-cosmic" : "" }
         ext.args2        = "--export csv"
         publishDir = [
diff --git a/modules.json b/modules.json
index b7ce82d85..b7a41b4df 100644
--- a/modules.json
+++ b/modules.json
@@ -45,6 +45,16 @@
                         "git_sha": "1037205706929921360f57e8042eae6819825e31",
                         "installed_by": ["modules"]
                     },
+                    "fusionreport/detect": {
+                        "branch": "master",
+                        "git_sha": "412d1e0614c3be267b8feb448588864a16a48293",
+                        "installed_by": ["modules"]
+                    },
+                    "fusionreport/download": {
+                        "branch": "master",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "installed_by": ["modules"]
+                    },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
diff --git a/modules/local/fusionreport/detect/meta.yml b/modules/local/fusionreport/detect/meta.yml
deleted file mode 100644
index ae3601dc5..000000000
--- a/modules/local/fusionreport/detect/meta.yml
+++ /dev/null
@@ -1,51 +0,0 @@
-name: fusionreport
-description: fusionreport
-keywords:
-  - sort
-tools:
-  - fusionreport:
-      description: Tool for parsing outputs from fusion detection tools
-      homepage: https://github.com/Clinical-Genomics/fusion-report
-      documentation: https://matq007.github.io/fusion-report/#/
-      doi: "10.1101/011650"
-      licence: ["GPL v3"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reference:
-      type: path
-      description: Path to fusionreport references
-      pattern: "*"
-  - arriba_fusions:
-      type: path
-      description: File
-      pattern: "*.fusions.tsv"
-  - starfusion_fusions:
-      type: path
-      description: File containing fusions from STARfusion
-      pattern: "*.starfusion.fusion_predictions.tsv"
-  - fusioncatcher_fusions:
-      type: path
-      description: File containing fusions from fusioncatcher
-      pattern: "*.fusions.tsv"
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - fusion_list:
-      type: file
-      description: File containing the summary of all fusions fed-in
-      pattern: "*.tsv"
-  - report:
-      type: file
-      description: HTML files
-      pattern: "*.html"
-
-authors:
-  - "@praveenraj2018, @rannick"
diff --git a/modules/local/fusionreport/detect/tests/nextflow.config b/modules/local/fusionreport/detect/tests/nextflow.config
deleted file mode 100644
index a1c327073..000000000
--- a/modules/local/fusionreport/detect/tests/nextflow.config
+++ /dev/null
@@ -1,10 +0,0 @@
-process {
-    withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args         =  "--no-cosmic"
-    }
-
-    withName: 'FUSIONREPORT' {
-        ext.args         = { {params.no_cosmic} ? "--no-cosmic" : "" }
-        ext.args2        = "--export csv"
-    }
-}
diff --git a/modules/local/fusionreport/download/environment.yml b/modules/local/fusionreport/download/environment.yml
deleted file mode 100644
index b3d78e480..000000000
--- a/modules/local/fusionreport/download/environment.yml
+++ /dev/null
@@ -1,6 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::fusion-report=4.0.0
-  - conda-forge::openpyxl=3.1.5
diff --git a/modules/local/fusionreport/download/meta.yml b/modules/local/fusionreport/download/meta.yml
deleted file mode 100644
index 21a15a893..000000000
--- a/modules/local/fusionreport/download/meta.yml
+++ /dev/null
@@ -1,35 +0,0 @@
-name: fusionreport_download
-description: Build DB for fusionreport
-keywords:
-  - sort
-tools:
-  - fusioncatcher:
-      description: Build DB for fusionreport
-      homepage: https://github.com/ndaniel/fusioncatcher/
-      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
-      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
-      doi: "10.1101/011650"
-      licence: ["GPL v3"]
-
-input:
-  - username:
-      type: value
-      description: Organism for which the data is downloaded from Ensembl database and built
-      pattern: "*"
-  - passwd:
-      type: value
-      description: Organism for which the data is downloaded from Ensembl database and built
-      pattern: "*"
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - reference:
-      type: directory
-      description: directory containing the genome resource files required for fusioncatcher
-      pattern: "fusioncatcher-genome"
-
-authors:
-  - "@praveenraj2018"
diff --git a/modules/local/fusionreport/detect/environment.yml b/modules/nf-core/fusionreport/detect/environment.yml
similarity index 71%
rename from modules/local/fusionreport/detect/environment.yml
rename to modules/nf-core/fusionreport/detect/environment.yml
index b3d78e480..f14a523ea 100644
--- a/modules/local/fusionreport/detect/environment.yml
+++ b/modules/nf-core/fusionreport/detect/environment.yml
@@ -2,5 +2,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fusion-report=4.0.0
+  - bioconda::fusion-report=4.0.1
   - conda-forge::openpyxl=3.1.5
diff --git a/modules/local/fusionreport/detect/main.nf b/modules/nf-core/fusionreport/detect/main.nf
similarity index 67%
rename from modules/local/fusionreport/detect/main.nf
rename to modules/nf-core/fusionreport/detect/main.nf
index 5143d53c8..0254100f5 100644
--- a/modules/local/fusionreport/detect/main.nf
+++ b/modules/nf-core/fusionreport/detect/main.nf
@@ -1,26 +1,25 @@
-process FUSIONREPORT {
+process FUSIONREPORT_DETECT {
     tag "$meta.id"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d99b7576d14caafd0494d6e2c1453edd161a933ccb62940701074128d3718bc5/data' :
-        'community.wave.seqera.io/library/fusion-report_openpyxl:77a2ba7e76ae0694'}"
-
+        'oras://community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:353260d00d0da0ec' :
+        'community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:78488bd99166aa9a'}"
 
     input:
-    tuple val(meta), path(arriba_fusions), path(starfusion_fusions),  path(fusioncatcher_fusions)
+    tuple val(meta), path(arriba_fusions), path(starfusion_fusions), path(fusioncatcher_fusions)
     tuple val(meta2), path(fusionreport_ref)
     val(tools_cutoff)
 
     output:
-    tuple val(meta), path("*fusionreport.tsv")                          , emit: fusion_list
-    tuple val(meta), path("*fusionreport_filtered.tsv")                 , emit: fusion_list_filtered
-    tuple val(meta), path("*index.html")                                , emit: report
-    tuple val(meta), path("*_*.html")                    , optional:true, emit: html
-    tuple val(meta), path("*.csv")                       , optional:true, emit: csv
-    tuple val(meta), path("*.json")                      , optional:true, emit: json
-    path "versions.yml"                                                 , emit: versions
+    tuple val(meta), path("*fusionreport.tsv")           , emit: fusion_list
+    tuple val(meta), path("*fusionreport_filtered.tsv")  , emit: fusion_list_filtered
+    tuple val(meta), path("*index.html")                 , emit: report
+    tuple val(meta), path("*_*.html")                    , emit: html                 , optional:true
+    tuple val(meta), path("*.csv")                       , emit: csv                  , optional:true
+    tuple val(meta), path("*.json")                      , emit: json                 , optional:true
+    path "versions.yml"                                  , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/nf-core/fusionreport/detect/meta.yml b/modules/nf-core/fusionreport/detect/meta.yml
new file mode 100644
index 000000000..9d28add25
--- /dev/null
+++ b/modules/nf-core/fusionreport/detect/meta.yml
@@ -0,0 +1,151 @@
+name: fusionreport_detect
+description: fusionreport_detect
+keywords:
+  - sort
+  - RNA-seq
+  - fusion report
+  - detect
+tools:
+  - fusionreport:
+      description: Tool for parsing outputs from fusion detection tools
+      homepage: https://github.com/Clinical-Genomics/fusion-report
+      documentation: https://matq007.github.io/fusion-report/#/
+      doi: "10.1101/011650"
+      licence: ["GPL v3"]
+      identifier: ""
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+    - arriba_fusions:
+        type: file
+        description: File
+        pattern: "*.fusions.tsv"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+    - starfusion_fusions:
+        type: file
+        description: File containing fusions from STARfusion
+        pattern: "*.starfusion.fusion_predictions.tsv"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+    - fusioncatcher_fusions:
+        type: file
+        description: File containing fusions from fusioncatcher
+        pattern: "*.fusions.tsv"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing sample information
+    - fusionreport_ref:
+        type: directory
+        description: File
+        pattern: "fusion_report_db"
+  - - tools_cutoff:
+        type: integer
+        description: Discard fusions detected by less than $tools_cutoff tools both for display in fusionreport html index and to consider in fusioninspector.
+
+output:
+  - fusion_list:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.tsv"
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+      - "*fusionreport.tsv":
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.tsv"
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+  - fusion_list_filtered:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.tsv"
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+      - "*fusionreport_filtered.tsv":
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.tsv"
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+  - report:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.index.html"
+          ontologies:
+            - edam: "http://edamontology.org/format_2331" # HTML
+      - "*index.html":
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.index.html"
+          ontologies:
+            - edam: "http://edamontology.org/format_2331" # HTML
+  - html:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*_*.html"
+          ontologies:
+            - edam: "http://edamontology.org/format_2331" # HTML
+      - "*_*.html":
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*_*.html"
+          ontologies:
+            - edam: "http://edamontology.org/format_2331" # HTML
+  - csv:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.csv"
+          ontologies:
+            - edam: "http://edamontology.org/format_3752" # CSV
+      - "*.csv":
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.csv"
+          ontologies:
+            - edam: "http://edamontology.org/format_3752" # CSV
+  - json:
+      - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.json"
+          ontologies:
+            - edam: "http://edamontology.org/format_3464" # JSON
+      - "*.json":
+          type: map
+          description: |
+            Groovy Map containing sample information
+          pattern: "*.json"
+          ontologies:
+            - edam: "http://edamontology.org/format_3464" # JSON
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@praveenraj2018, @rannick"
diff --git a/modules/local/fusionreport/detect/tests/main.nf.test b/modules/nf-core/fusionreport/detect/tests/main.nf.test
similarity index 64%
rename from modules/local/fusionreport/detect/tests/main.nf.test
rename to modules/nf-core/fusionreport/detect/tests/main.nf.test
index 9fc895403..3ff9b6877 100644
--- a/modules/local/fusionreport/detect/tests/main.nf.test
+++ b/modules/nf-core/fusionreport/detect/tests/main.nf.test
@@ -1,11 +1,14 @@
 nextflow_process {
 
-    name "Test Process FUSIONREPORT"
+    name "Test Process FUSIONREPORT_DETECT"
     script "../main.nf"
-    process "FUSIONREPORT"
+    process "FUSIONREPORT_DETECT"
     tag "modules"
     tag "modules_local"
     tag "fusionreport"
+    tag "fusionreport/detect"
+    tag "modules_nfcore"
+    tag "fusionreport/download"
 
 
     test("FUSIONREPORT - arriba - starfusion - fusioncatcher") {
@@ -27,7 +30,6 @@ nextflow_process {
 
                 input[0] = [
                     [ id:'test_sample' ], // meta map
-                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz"),
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv"),
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv"),
                     file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
@@ -38,11 +40,14 @@ nextflow_process {
                 """
             }
             params {
-                arriba          = true
-                starfusion      = true
-                fusioncatcher   = true
-                no_cosmic       = true
-                outdir          = "$outputDir"
+                arriba                     = true
+                starfusion                 = true
+                fusioncatcher              = true
+                no_cosmic                  = true
+                outdir                     = "$outputDir"
+                fusionreport_download_args = '--no-cosmic'
+                fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
+                fusionreport_detect_args2  = "--export csv"
             }
         }
 
@@ -84,23 +89,26 @@ nextflow_process {
 
                 input[0] = [
                     [ id:'test_sample' ], // meta map
-                    file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/reads_1.fq.gz"),
-                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv"),
-                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv"),
-                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/arriba.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/starfusion.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/fusioncatcher.txt', checkIfExists: true)
                 ]
 
                 input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
 
                 input[2] = 1
+
                 """
             }
         params {
-                arriba          = true
-                starfusion      = true
-                fusioncatcher   = true
-                no_cosmic       = true
-                outdir          = "$outputDir"
+                arriba                     = true
+                starfusion                 = true
+                fusioncatcher              = true
+                no_cosmic                  = true
+                outdir                     = "$outputDir"
+                fusionreport_download_args = '--no-cosmic'
+                fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
+                fusionreport_detect_args2  = "--export csv"
             }
         }
 
@@ -111,6 +119,4 @@ nextflow_process {
             )
         }
     }
-
-
-}
+}
\ No newline at end of file
diff --git a/modules/local/fusionreport/detect/tests/main.nf.test.snap b/modules/nf-core/fusionreport/detect/tests/main.nf.test.snap
similarity index 92%
rename from modules/local/fusionreport/detect/tests/main.nf.test.snap
rename to modules/nf-core/fusionreport/detect/tests/main.nf.test.snap
index c20e3ec41..7b71aa358 100644
--- a/modules/local/fusionreport/detect/tests/main.nf.test.snap
+++ b/modules/nf-core/fusionreport/detect/tests/main.nf.test.snap
@@ -22,7 +22,7 @@
                     {
                         "id": "test_sample"
                     },
-                    "test_sample.fusions.csv:md5,49f378c2112d7e0b3b17d9095c79e6bd"
+                    "test_sample.fusions.csv:md5,ee6c6838110d68bc75a13c19dca0a321"
                 ]
             ],
             "test_sample_fusionreport_index.html",
@@ -59,18 +59,15 @@
             "versions.yml"
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.4"
         },
-        "timestamp": "2024-12-06T14:23:44.59690452"
+        "timestamp": "2025-06-26T16:15:03.892288278"
     },
     "FUSIONREPORT - arriba - starfusion - fusioncatcher - stub": {
         "content": [
             {
                 "0": [
-                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
-                ],
-                "1": [
                     [
                         {
                             "id": "test_sample"
@@ -78,7 +75,7 @@
                         "test_sample.fusionreport.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "2": [
+                "1": [
                     [
                         {
                             "id": "test_sample"
@@ -86,7 +83,7 @@
                         "test_sample.fusionreport_filtered.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "3": [
+                "2": [
                     [
                         {
                             "id": "test_sample"
@@ -94,7 +91,7 @@
                         "test_sample_fusionreport_index.html:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "4": [
+                "3": [
                     [
                         {
                             "id": "test_sample"
@@ -105,7 +102,7 @@
                         ]
                     ]
                 ],
-                "5": [
+                "4": [
                     [
                         {
                             "id": "test_sample"
@@ -113,7 +110,7 @@
                         "test_sample.fusions.csv:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "6": [
+                "5": [
                     [
                         {
                             "id": "test_sample"
@@ -121,6 +118,9 @@
                         "test_sample.fusions.json:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
+                "6": [
+                    "versions.yml:md5,947ac2c0ed1623930f951f4f4ee3501e"
+                ],
                 "csv": [
                     [
                         {
@@ -173,14 +173,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,6bd28f2526774f519a7627a30c6a7f2f"
+                    "versions.yml:md5,947ac2c0ed1623930f951f4f4ee3501e"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.4"
         },
-        "timestamp": "2024-12-10T15:13:58.414161"
+        "timestamp": "2025-07-01T14:42:59.809164059"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/fusionreport/detect/tests/nextflow.config b/modules/nf-core/fusionreport/detect/tests/nextflow.config
new file mode 100644
index 000000000..ed07a62ee
--- /dev/null
+++ b/modules/nf-core/fusionreport/detect/tests/nextflow.config
@@ -0,0 +1,10 @@
+process {
+    withName: 'FUSIONREPORT_DOWNLOAD' {
+        ext.args         =  params.fusionreport_download_args
+    }
+
+    withName: 'FUSIONREPORT_DETECT' {
+        ext.args         = params.fusionreport_detect_args
+        ext.args2        = params.fusionreport_detect_args2
+    }
+}
diff --git a/modules/nf-core/fusionreport/download/environment.yml b/modules/nf-core/fusionreport/download/environment.yml
new file mode 100644
index 000000000..ae86909d1
--- /dev/null
+++ b/modules/nf-core/fusionreport/download/environment.yml
@@ -0,0 +1,18 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::fusion-report=4.0.1
+  - conda-forge::beautifulsoup4=4.13.4
+  - conda-forge::click=8.2.1
+  - conda-forge::colorlog=6.9.0
+  - conda-forge::jinja2=3.1.6
+  - conda-forge::markupsafe=3.0.2
+  - conda-forge::numpy=2.3.0
+  - conda-forge::openpyxl=3.1.5
+  - conda-forge::pandas=2.3.0
+  - conda-forge::pyyaml=6.0.2
+  - conda-forge::requests=2.32.4
+  - conda-forge::tabulate=0.9.0
+  - conda-forge::tqdm=4.67.1
+  - conda-forge::xlrd=2.0.1
diff --git a/modules/local/fusionreport/download/main.nf b/modules/nf-core/fusionreport/download/main.nf
similarity index 80%
rename from modules/local/fusionreport/download/main.nf
rename to modules/nf-core/fusionreport/download/main.nf
index 839c20151..a74a5f2eb 100644
--- a/modules/local/fusionreport/download/main.nf
+++ b/modules/nf-core/fusionreport/download/main.nf
@@ -4,13 +4,16 @@ process FUSIONREPORT_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d9/d99b7576d14caafd0494d6e2c1453edd161a933ccb62940701074128d3718bc5/data' :
-        'community.wave.seqera.io/library/fusion-report_openpyxl:77a2ba7e76ae0694'}"
+        'oras://community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:353260d00d0da0ec' :
+        'community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:78488bd99166aa9a'}"
 
     output:
     tuple val(meta), path("fusion_report_db"), emit: fusionreport_ref
     path "versions.yml"                      , emit: versions
 
+    when:
+    task.ext.when == null || task.ext.when
+
     script:
     meta = [id: 'fusion_report_db']
     def args = task.ext.args ?: ''
diff --git a/modules/nf-core/fusionreport/download/meta.yml b/modules/nf-core/fusionreport/download/meta.yml
new file mode 100644
index 000000000..8922d7549
--- /dev/null
+++ b/modules/nf-core/fusionreport/download/meta.yml
@@ -0,0 +1,36 @@
+name: fusionreport_download
+description: Build DB for fusionreport
+keywords:
+  - sort
+  - RNA-seq
+  - fusion_report
+  - download
+tools:
+  - fusionreport:
+      description: Generate an interactive summary report from fusion detection tools.
+      homepage: https://github.com/Clinical-Genomics/fusion-report
+      documentation: https://github.com/Clinical-Genomics/fusion-report/blob/master/README.md
+      tool_dev_url: https://github.com/Clinical-Genomics/fusion-report
+      licence: ["GPL v3"]
+      identifier: ""
+
+output:
+  fusionreport_ref:
+    - - meta:
+          type: string
+          description: directory containing the genome resource files required for fusioncatcher
+          pattern: "fusion_report_db"
+      - fusion_report_db:
+          type: directory
+          description: directory containing the genome resource files required for fusioncatcher
+          pattern: "fusion_report_db"
+
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@praveenraj2018"
diff --git a/modules/local/fusionreport/download/tests/main.nf.test b/modules/nf-core/fusionreport/download/tests/main.nf.test
similarity index 83%
rename from modules/local/fusionreport/download/tests/main.nf.test
rename to modules/nf-core/fusionreport/download/tests/main.nf.test
index 35af2a52b..f05728932 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test
+++ b/modules/nf-core/fusionreport/download/tests/main.nf.test
@@ -3,11 +3,18 @@ nextflow_process {
     name "Test Process FUSIONREPORT_DOWNLOAD"
     script "../main.nf"
     process "FUSIONREPORT_DOWNLOAD"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "fusionreport"
+    tag "fusionreport/download"
 
     test("Download fusionreport databases") {
         config './nextflow.config'
 
         when {
+            params {
+                module_args = '--no-cosmic'
+            }
             process {
                 """
                 // This process doesn't have any inputs
@@ -34,6 +41,9 @@ nextflow_process {
         options "-stub"
 
         when {
+            params {
+                module_args = '--no-cosmic'
+            }
             process {
                 """
                 // This process doesn't have any inputs
@@ -48,4 +58,4 @@ nextflow_process {
             )
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/fusionreport/download/tests/main.nf.test.snap b/modules/nf-core/fusionreport/download/tests/main.nf.test.snap
similarity index 74%
rename from modules/local/fusionreport/download/tests/main.nf.test.snap
rename to modules/nf-core/fusionreport/download/tests/main.nf.test.snap
index 722d8c68b..ab161336a 100644
--- a/modules/local/fusionreport/download/tests/main.nf.test.snap
+++ b/modules/nf-core/fusionreport/download/tests/main.nf.test.snap
@@ -1,19 +1,19 @@
 {
     "Download fusionreport databases": {
         "content": [
-            "fusiongdb2.db:md5,e1ac123a744e515d3e5f85b8344d526a",
-            "mitelman.db:md5,1363795c97f77c641065ecd9ad0e484a",
+            "fusiongdb2.db:md5,9dec75bfc146f56b36e5a84358f9e515",
+            "mitelman.db:md5,d31478ed66bd6623172852d94132e933",
             true,
             true,
             [
-                "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
+                "versions.yml:md5,620c6e01391800a7e7b717d7a3024003"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.4"
         },
-        "timestamp": "2024-12-05T19:27:38.99855171"
+        "timestamp": "2025-06-30T15:25:09.631428688"
     },
     "Create stub files": {
         "content": [
@@ -33,7 +33,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
+                    "versions.yml:md5,620c6e01391800a7e7b717d7a3024003"
                 ],
                 "fusionreport_ref": [
                     [
@@ -50,14 +50,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,fa5f13c563f431912048c1802b5a0c74"
+                    "versions.yml:md5,620c6e01391800a7e7b717d7a3024003"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.4"
         },
-        "timestamp": "2024-12-10T15:05:22.781845"
+        "timestamp": "2025-06-27T19:34:10.41756869"
     }
 }
\ No newline at end of file
diff --git a/modules/local/fusionreport/download/tests/nextflow.config b/modules/nf-core/fusionreport/download/tests/nextflow.config
similarity index 55%
rename from modules/local/fusionreport/download/tests/nextflow.config
rename to modules/nf-core/fusionreport/download/tests/nextflow.config
index 7c1ffb507..505233327 100644
--- a/modules/local/fusionreport/download/tests/nextflow.config
+++ b/modules/nf-core/fusionreport/download/tests/nextflow.config
@@ -1,5 +1,5 @@
 process {
     withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args         =  "--no-cosmic"
+        ext.args         =  params.module_args
     }
 }
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 6858cf894..5751627ff 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -6,7 +6,7 @@
 
 include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode_download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../../../modules/local/fusioncatcher/download/main'
-include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/local/fusionreport/download/main'
+include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/nf-core/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../../../modules/local/starfusion/build/main'
 include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
diff --git a/subworkflows/local/fusionreport_workflow/main.nf b/subworkflows/local/fusionreport_workflow/main.nf
index c4a896dd7..1235d724f 100644
--- a/subworkflows/local/fusionreport_workflow/main.nf
+++ b/subworkflows/local/fusionreport_workflow/main.nf
@@ -1,4 +1,4 @@
-include { FUSIONREPORT      }     from '../../../modules/local/fusionreport/detect/main'
+include { FUSIONREPORT_DETECT      }     from '../../../modules/nf-core/fusionreport/detect/main'
 
 
 workflow FUSIONREPORT_WORKFLOW {
@@ -17,12 +17,12 @@ workflow FUSIONREPORT_WORKFLOW {
             .join(starfusion_fusions, failOnMismatch:true, failOnDuplicate:true)
             .join(fusioncatcher_fusions, failOnMismatch:true, failOnDuplicate:true)
 
-        FUSIONREPORT(ch_fusions, fusionreport_ref, params.tools_cutoff)
-        ch_fusion_list = FUSIONREPORT.out.fusion_list
-        ch_fusion_list_filtered = FUSIONREPORT.out.fusion_list_filtered
-        ch_versions = ch_versions.mix(FUSIONREPORT.out.versions)
-        ch_report = FUSIONREPORT.out.report
-        ch_csv = FUSIONREPORT.out.csv
+        FUSIONREPORT_DETECT(ch_fusions, fusionreport_ref, params.tools_cutoff)
+        ch_fusion_list = FUSIONREPORT_DETECT.out.fusion_list
+        ch_fusion_list_filtered = FUSIONREPORT_DETECT.out.fusion_list_filtered
+        ch_versions = ch_versions.mix(FUSIONREPORT_DETECT.out.versions)
+        ch_report = FUSIONREPORT_DETECT.out.report
+        ch_csv = FUSIONREPORT_DETECT.out.csv
 
     emit:
         versions                 = ch_versions

From d76bef9e4f64b390f62e87ba1831e29b26a38437 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Tue, 8 Jul 2025 14:36:39 +0000
Subject: [PATCH 582/887] Update build test

---
 .../local/fusionreport_workflow/tests/main.nf.test     |  2 +-
 tests/test_build.nf.test.snap                          | 10 +++++-----
 2 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
index c5e2a54ca..63e44f1f8 100644
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
@@ -12,7 +12,7 @@ nextflow_workflow {
 
         setup {
             run("FUSIONREPORT_DOWNLOAD") {
-                script "../../../../modules/local/fusionreport/download/main.nf"
+                script "../../../../modules/nf-core/fusionreport/download/main.nf"
                 process {
                     """
                     """
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 91f926bb9..d92113b8a 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -7,7 +7,7 @@
                     "arriba_download": "2.4.0"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "4.0.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -277,9 +277,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T14:33:25.428259571"
+        "timestamp": "2025-07-08T14:00:33.582495752"
     }
-}
+}
\ No newline at end of file

From 04f37e51563143419d4fa475dc096d5089d019a5 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Tue, 8 Jul 2025 15:09:17 +0000
Subject: [PATCH 583/887] Update stub tests

---
 tests/test_stub.nf.test.snap     | 28 ++++++++++++++--------------
 tests/test_stub_bam.nf.test.snap | 24 ++++++++++++------------
 2 files changed, 26 insertions(+), 26 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 6ea02a74e..a2ecc9705 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -516,11 +516,11 @@
                 "FUSIONINSPECTOR": {
                     "STAR-Fusion": null
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                "FUSIONREPORT_DETECT": {
+                    "fusion_report": "4.0.1"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "4.0.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -875,9 +875,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T14:33:17.006992"
+        "timestamp": "2025-07-08T15:06:14.950970078"
     },
     "stub test salmon": {
         "content": [
@@ -1220,11 +1220,11 @@
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                "FUSIONREPORT_DETECT": {
+                    "fusion_report": "4.0.1"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "4.0.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1460,7 +1460,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T11:12:10.068978"
+        "timestamp": "2025-07-08T15:04:39.201458666"
     },
     "stub test fastp": {
         "content": [
@@ -1732,11 +1732,11 @@
                 "FUSIONINSPECTOR": {
                     "STAR-Fusion": null
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                "FUSIONREPORT_DETECT": {
+                    "fusion_report": "4.0.1"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "4.0.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -2176,8 +2176,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T14:19:12.460594"
+        "timestamp": "2025-07-08T15:01:11.405363456"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 4ddbbbc9f..16f2f89c3 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -438,11 +438,11 @@
                 "FUSIONINSPECTOR": {
                     "STAR-Fusion": null
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                "FUSIONREPORT_DETECT": {
+                    "fusion_report": "4.0.1"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "4.0.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -775,7 +775,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T17:14:35.061729"
+        "timestamp": "2025-07-08T14:58:49.830786101"
     },
     "stub test salmon": {
         "content": [
@@ -1113,11 +1113,11 @@
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                "FUSIONREPORT_DETECT": {
+                    "fusion_report": "4.0.1"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "4.0.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1326,7 +1326,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T14:17:58.683212"
+        "timestamp": "2025-07-08T14:57:00.562428474"
     },
     "stub test fastp": {
         "content": [
@@ -1554,11 +1554,11 @@
                 "FUSIONINSPECTOR": {
                     "STAR-Fusion": null
                 },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
+                "FUSIONREPORT_DETECT": {
+                    "fusion_report": "4.0.1"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.0"
+                    "fusion_report": "4.0.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1976,6 +1976,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T16:55:37.86841"
+        "timestamp": "2025-07-08T14:53:35.614724758"
     }
 }
\ No newline at end of file

From 1c781dcf4f5922f36d91f8ca190b0676cea5e9b9 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Tue, 8 Jul 2025 15:23:02 +0000
Subject: [PATCH 584/887] Update `CHANGELOG.md`

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4f23a6476..2b168b7c3 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -82,6 +82,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   - `all` => This will automatically run all of the above tools
 - Updated all `wget` containers to `conda-forge::wget=1.21.4` [#655](https://github.com/nf-core/rnafusion/pull/655)
 - Using FusionInspector abridged output (that now contains coding effects) for downstream analysis [#669](https://github.com/nf-core/rnafusion/pull/669)
+- Replaced local `FUSIONREPORT` and `FUSIONREPORT_DOWNLOAD` with `FUSIONREPORT_DETECT` and `FUSIONREPORT_DOWNLOAD` from nf-core [#703](https://github.com/nf-core/rnafusion/pull/703)
 
 ### Fixed
 

From 66b06fa8eb865a85acbde41727ff1fcd7ec1d5c5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 9 Jul 2025 13:00:32 +0200
Subject: [PATCH 585/887] update stub

---
 tests/test_stub_bam.nf.test.snap | 60 ++++++++++++++++----------------
 1 file changed, 30 insertions(+), 30 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 4ddbbbc9f..4edb078f8 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -307,10 +307,10 @@
             14,
             {
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -381,10 +381,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/gencode",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
@@ -411,10 +411,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:26:43.570224768"
+        "timestamp": "2025-07-08T12:57:24.512309"
     },
     "stub test fusioninspector": {
         "content": [
@@ -424,10 +424,10 @@
                     "agat": "v1.4.1"
                 },
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.4.0"
@@ -652,10 +652,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/cosmic.db",
@@ -775,7 +775,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T17:14:35.061729"
+        "timestamp": "2025-07-08T13:15:34.702165"
     },
     "stub test salmon": {
         "content": [
@@ -1098,10 +1098,10 @@
             20,
             {
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1204,10 +1204,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/cosmic.db",
@@ -1326,7 +1326,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T14:17:58.683212"
+        "timestamp": "2025-07-08T13:08:14.629821"
     },
     "stub test fastp": {
         "content": [
@@ -1521,10 +1521,10 @@
                     "agat": "v1.4.1"
                 },
                 "ARRIBA_ARRIBA": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
+                    "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.4.0"
@@ -1811,10 +1811,10 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/cosmic.db",
@@ -1976,6 +1976,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T16:55:37.86841"
+        "timestamp": "2025-07-08T12:55:23.941369"
     }
 }
\ No newline at end of file

From f3e79b999f3e099fd28207b2ce5f1bdd2b894f20 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 10 Jul 2025 12:39:17 +0000
Subject: [PATCH 586/887] chore: remove subworkflow with only one module

---
 CHANGELOG.md           |  1 +
 workflows/rnafusion.nf | 20 +++++++++++++-------
 2 files changed, 14 insertions(+), 7 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4f23a6476..1ed361b50 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -108,6 +108,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed parameter `--build_references`
 - Removed fusioncatcher build [#650](https://github.com/nf-core/rnafusion/pull/650)
 - Removed subworkflow with less than two modules: `ARRIBA_WORKFLOW` [#692](https://github.com/nf-core/rnafusion/pull/692)
+- Removed subworkflow with less than two modules: `CTATSPLICING_WORKFLOW` [#704](https://github.com/nf-core/rnafusion/pull/704)
 
 ### Parameters
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index c8204bcb4..8d9743a8b 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -20,12 +20,12 @@ include { SALMON_QUANT                  }   from '../modules/nf-core/salmon/quan
 include { SAMTOOLS_CONVERT              }   from '../modules/nf-core/samtools/convert/main'
 include { paramsSummaryMap              }   from 'plugin/nf-schema'
 include { FASTQ_ALIGN_STAR              }   from '../subworkflows/local/fastq_align_star'
-include { CTATSPLICING_WORKFLOW         }   from '../subworkflows/local/ctatsplicing_workflow'
 include { paramsSummaryMultiqc          }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { ARRIBA_ARRIBA                 }   from '../modules/nf-core/arriba/arriba/main'
+include { CTATSPLICING_STARTOCANCERINTRONS } from '../modules/local/ctatsplicing/startocancerintrons'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -195,17 +195,23 @@ workflow RNAFUSION {
         }
 
         //
-        // SUBWORKFLOW: Run CTAT-SPLICING
+        // MODULE: Run CTAT-SPLICING
         //
 
         if(tools.contains("ctatsplicing")) {
-            CTATSPLICING_WORKFLOW(
-                ch_star_splice_junctions,
-                ch_star_junctions,
-                ch_aligned_reads,
+            def ch_ctatsplicing_input = ch_star_splice_junctions
+                .join(ch_star_junctions, failOnMismatch:true, failOnDuplicate:true)
+                .join(ch_aligned_reads, failOnMismatch:true, failOnDuplicate:true)
+                .map { meta, split_junction, junction, bam, bai ->
+                    [ meta, split_junction, junction, bam, bai ]
+                }
+
+            CTATSPLICING_STARTOCANCERINTRONS(
+                ch_ctatsplicing_input,
                 BUILD_REFERENCES.out.starfusion_ref
             )
-            ch_versions = ch_versions.mix(CTATSPLICING_WORKFLOW.out.versions)
+
+            ch_versions = ch_versions.mix(CTATSPLICING_STARTOCANCERINTRONS.out.versions.first())
         }
 
         //

From 44e9a215334206caeb49a8439b8b289e21315a87 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 10 Jul 2025 14:41:39 +0200
Subject: [PATCH 587/887] update test

---
 tests/test_build.nf.test      |   5 +
 tests/test_build.nf.test.snap | 266 +---------------------------------
 2 files changed, 12 insertions(+), 259 deletions(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 6fb5cc32a..72f62ee3f 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -12,6 +12,11 @@ nextflow_pipeline {
         when {
             params {
                 outdir = "$outputDir"
+                outdir           = "$outputDir"
+                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                input            = "${projectDir}/tests/yml/bam.yml"
+                tools            = "ctatsplicing,starfusion,arriba,fusionreport,salmon"
+                no_cosmic        = true
             }
         }
 
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 2dbc57023..9af3fcc7d 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -1,285 +1,33 @@
 {
     "Test build references": {
         "content": [
-            12,
+            1,
             {
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.4.0"
-                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "4.0.0"
                 },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GFFREAD": {
-                    "gffread": "0.12.7"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "HGNC_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "SALMON_INDEX": {
-                    "salmon": "1.10.3"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "gatk4",
-                "gatk4/minigenome.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
                 "references/fusion_report_db/fusion_report.log",
                 "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/gencode",
-                "references/gencode/minigenome.fa.fai",
-                "references/gencode/minigenome.gtf.interval_list",
-                "references/gencode/minigenome.gtf.refflat",
-                "references/gffread",
-                "references/gffread/minigenome.gtf.fasta",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz"
+                "references/fusion_report_db/mitelman.db"
             ],
             [
-                "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
-                "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
-                "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
-                "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
-                "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
-                "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
-                "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
-                "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
-                "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
-                "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
-                "info.json:md5,d91d8a954afcb4be9fe7bfb3e84b5be6",
-                "mphf.bin:md5,b364192c6c73de4c38ec2ed0750f5155",
-                "rank.bin:md5,3fe9c2dc9da7faff6362510c4f29e87d",
-                "refAccumLengths.bin:md5,26d9405e7591ec9a606caa36a094941a",
-                "reflengths.bin:md5,187a4a0dee316c5dbf440fa3c58d9eec",
-                "refseq.bin:md5,712c4a8d988c15016ea746db7022e549",
-                "versionInfo.json:md5,d2c799050e81aa6e282ac8a73e773941",
-                "Genome:md5,1621486e3b854fefa37c0b4e77275233",
-                "SA:md5,9b6b936f3a8a1232c4fc5533daf9401d",
-                "SAindex:md5,8b2025227b78fdc83e3e5b17c0f538f7",
-                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
-                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
-                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
-                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
-                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
-                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
-                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "sjdbInfo.txt:md5,f044cb1cd38af66c832f3afc4b1655cb",
-                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
-                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
-                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
-                "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
-                "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
-                "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
-                "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
-                "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
-                "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
-                "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
-                "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
-                "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
-                "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                "SA:md5,7dd9083264be9c6a2194d990bc10d237",
-                "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
-                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
-                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
-                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
-                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
-                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
-                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
-                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
-                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
-                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
-                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
-                "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
+                "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T14:33:25.428259571"
+        "timestamp": "2025-07-09T14:55:30.278624"
     }
-}
+}
\ No newline at end of file

From bed623395d787411e270b5814125e55b42911fd8 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 10 Jul 2025 14:28:45 +0000
Subject: [PATCH 588/887] refact: remove subworkflow with less than 2 modules

---
 CHANGELOG.md                                  |   1 +
 .../local/starfusion_workflow/main.nf         |  28 -----
 .../starfusion_workflow/tests/main.nf.test    | 111 ------------------
 .../tests/main.nf.test.snap                   |  38 ------
 .../starfusion_workflow/tests/nextflow.config |  33 ------
 workflows/rnafusion.nf                        |  26 ++--
 6 files changed, 17 insertions(+), 220 deletions(-)
 delete mode 100644 subworkflows/local/starfusion_workflow/main.nf
 delete mode 100644 subworkflows/local/starfusion_workflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
 delete mode 100644 subworkflows/local/starfusion_workflow/tests/nextflow.config

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4f23a6476..eebfdc099 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -108,6 +108,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed parameter `--build_references`
 - Removed fusioncatcher build [#650](https://github.com/nf-core/rnafusion/pull/650)
 - Removed subworkflow with less than two modules: `ARRIBA_WORKFLOW` [#692](https://github.com/nf-core/rnafusion/pull/692)
+- Removed subworkflow with less than two modules: `STARFUSION_WORKFLOW` [#707](https://github.com/nf-core/rnafusion/pull/707)
 
 ### Parameters
 
diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
deleted file mode 100644
index 53f0dd8a4..000000000
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ /dev/null
@@ -1,28 +0,0 @@
-include { STARFUSION                                            }   from '../../../modules/local/starfusion/detect/main'
-
-workflow STARFUSION_WORKFLOW {
-    take:
-        junctions           // channel: [ meta, junctions ]
-        ch_starfusion_ref
-        starfusion_fusions
-
-    main:
-        def ch_versions = Channel.empty()
-        def ch_starfusion_fusions = Channel.empty()
-
-        if (starfusion_fusions){
-            fusions = file(starfusion_fusions, checkIfExists:true)
-            ch_starfusion_fusions = junctions.map { meta, _junc -> [ meta, fusions ] }
-        } else {
-            STARFUSION(
-                junctions.map { meta, junc -> [ meta, [], junc ]},
-                ch_starfusion_ref.map { it -> it[1] }
-            )
-            ch_versions = ch_versions.mix(STARFUSION.out.versions)
-            ch_starfusion_fusions = STARFUSION.out.fusions
-        }
-    emit:
-        fusions               = ch_starfusion_fusions
-        versions              = ch_versions
-    }
-
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
deleted file mode 100644
index af6b117ff..000000000
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test
+++ /dev/null
@@ -1,111 +0,0 @@
-nextflow_workflow {
-
-    name "Test Subworkflow STARFUSION_WORKFLOW"
-    script "../main.nf"
-    workflow "STARFUSION_WORKFLOW"
-    tag "subworkflow"
-    tag "star"
-    tag "star/genomegenerate"
-    tag "star/align"
-
-
-    test("STARFUSION_WORKFLOW - Homo sapiens - FASTQs minigenome") {
-        config './nextflow.config'
-
-        setup {
-            run("STAR_GENOMEGENERATE") {
-                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
-                process {
-                """
-                    // FASTA
-                    input[0] = Channel.fromPath(
-                            "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-
-                    // GTF
-                    input[1] = Channel.fromPath(
-                            "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-                    """
-                }
-            }
-
-            run("STARFUSION_BUILD") {
-            script "../../../../modules/local/starfusion/build/main.nf"
-            process {
-                """
-                input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
-                ]
-                input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
-                ]
-
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
-                """
-            }
-        }
-        }
-
-        when {
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [ id: "test_fastqs" ],
-                        [
-                            file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz", checkIfExists: true),
-                            file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz", checkIfExists: true)
-                        ]
-                    ] )
-
-                // ch_gtf
-                input[1] =
-                    Channel.fromPath(
-                        "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_starindex_ref
-                input[2] = STAR_GENOMEGENERATE.out.index
-
-                // ch_fasta
-                input[3] =
-                    Channel.fromPath(
-                        "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_starfusion_ref
-                input[4] = STARFUSION_BUILD.out.reference
-
-                """
-            }
-            params {
-                starfusion = true
-                cram = 'starfusion'
-                outdir = "$outputDir"
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(
-                    workflow.out.fusions,
-                    workflow.out.versions
-                ).match() }
-            )
-        }
-    }
-
-
-
-
-}
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap b/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 5bc897e7f..000000000
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,38 +0,0 @@
-{
-    "STARFUSION_WORKFLOW - Homo sapiens - FASTQs minigenome": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test_fastqs"
-                    },
-                    "test_fastqs.starfusion.fusion_predictions.tsv:md5,abe17134a231642edf9351e4964e8a97"
-                ]
-            ],
-            "test_fastqs.Log.final.out",
-            [
-                [
-                    {
-                        "id": "test_fastqs"
-                    },
-                    "test_fastqs.ReadsPerGene.out.tab:md5,8e0d42deeea09924d5c7ba3147bbfd78"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                "versions.yml:md5,afde4f2fd6056df81e322b3c35ab7a8a",
-                "versions.yml:md5,e53f1ec32bc78a33f99892e42274833a"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2024-12-23T18:25:52.838411738"
-    }
-}
\ No newline at end of file
diff --git a/subworkflows/local/starfusion_workflow/tests/nextflow.config b/subworkflows/local/starfusion_workflow/tests/nextflow.config
deleted file mode 100644
index 2f955abc4..000000000
--- a/subworkflows/local/starfusion_workflow/tests/nextflow.config
+++ /dev/null
@@ -1,33 +0,0 @@
-process {
-    withName: 'STAR_FOR_STARFUSION' {
-        ext.args = '--twopassMode Basic \
-        --outReadsUnmapped None \
-        --readFilesCommand zcat \
-        --outSAMtype BAM SortedByCoordinate \
-        --outSAMstrandField intronMotif \
-        --outSAMunmapped Within \
-        --chimSegmentMin 12 \
-        --chimJunctionOverhangMin 8 \
-        --chimOutJunctionFormat 1 \
-        --alignSJDBoverhangMin 10 \
-        --alignMatesGapMax 100000 \
-        --alignIntronMax 100000 \
-        --alignSJstitchMismatchNmax 5 -1 5 5 \
-        --chimMultimapScoreRange 3 \
-        --chimScoreJunctionNonGTAG -4 \
-        --chimMultimapNmax 20 \
-        --chimNonchimScoreDropMin 10 \
-        --peOverlapNbasesMin 12 \
-        --peOverlapMMp 0.1 \
-        --alignInsertionFlush Right \
-        --alignSplicedMateMapLminOverLmate 0 \
-        --alignSplicedMateMapLmin 30 \
-        --chimOutType Junctions \
-        --quantMode GeneCounts'
-    }
-
-        withName: 'SAMTOOLS_INDEX_FOR_STARFUSION_CRAM' {
-        ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
-    }
-
-}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index c8204bcb4..4fedfdefa 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -8,7 +8,7 @@ include { BUILD_REFERENCES              }   from '../subworkflows/local/build_re
 include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
 include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow/main'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
-include { STARFUSION_WORKFLOW           }   from '../subworkflows/local/starfusion_workflow'
+include { STARFUSION                    }   from '../modules/local/starfusion/detect/main'
 include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
 include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
@@ -241,21 +241,27 @@ workflow RNAFUSION {
 
 
         //
-        // SUBWORKFLOW: Run STAR alignment and StarFusion
+        // MODULE: Run StarFusion
         //
 
         def ch_starfusion_fusions = ch_samplesheet.map { it -> [it[0], []] } // Set starfusion fusions to empty by default
-        if(tools.contains("starfusion")) {
+        if (tools.contains("starfusion")) {
             fusions_created = true
-            STARFUSION_WORKFLOW (
-                ch_star_junctions,
-                BUILD_REFERENCES.out.starfusion_ref,
-                params.starfusion_fusions
-            )
-            ch_versions             = ch_versions.mix(STARFUSION_WORKFLOW.out.versions)
-            ch_starfusion_fusions   = STARFUSION_WORKFLOW.out.fusions
+
+            if (params.starfusion_fusions) {
+                def fusions = file(params.starfusion_fusions, checkIfExists:true)                   // TODO: What is fusions used for? Remove?
+                ch_starfusion_fusions = ch_star_junctions.map { meta, _junc -> [ meta, fusions ] }  // TODO: We use the junctions but not the provided fusions
+            } else {
+                STARFUSION(
+                    ch_star_junctions.map { meta, junc -> [ meta, [], junc ] },
+                    BUILD_REFERENCES.out.starfusion_ref.map { it -> it[1] }
+                )
+                ch_versions = ch_versions.mix(STARFUSION.out.versions)
+                ch_starfusion_fusions = STARFUSION.out.fusions
+            }
         }
 
+
         //
         // SUBWORKFLOW: Run FusionCatcher
         //

From e3353171c89537dc957caee64e39921dea754a0a Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@scilifelab.se>
Date: Thu, 10 Jul 2025 15:34:12 +0000
Subject: [PATCH 589/887] update arriba visualisation

---
 modules/local/arriba/visualisation/environment.yml | 8 ++++++++
 modules/local/arriba/visualisation/main.nf         | 6 +++---
 2 files changed, 11 insertions(+), 3 deletions(-)
 create mode 100644 modules/local/arriba/visualisation/environment.yml

diff --git a/modules/local/arriba/visualisation/environment.yml b/modules/local/arriba/visualisation/environment.yml
new file mode 100644
index 000000000..b30731312
--- /dev/null
+++ b/modules/local/arriba/visualisation/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::arriba=2.5.0
+  - conda-forge::wget=1.21.4
diff --git a/modules/local/arriba/visualisation/main.nf b/modules/local/arriba/visualisation/main.nf
index 293247328..c5fed05a0 100644
--- a/modules/local/arriba/visualisation/main.nf
+++ b/modules/local/arriba/visualisation/main.nf
@@ -2,10 +2,10 @@ process ARRIBA_VISUALISATION {
     tag "$meta.id"
     label 'process_medium'
 
-    conda "bioconda::arriba=2.4.0"
+    conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
-        'biocontainers/arriba:2.4.0--h0033a41_2' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/27/27475cdcdbcc8c0ffb6b5ca8c2e6567dbe490edb96f5df4e8f01f4f95912dcd3/data' :
+        'community.wave.seqera.io/library/arriba_wget:a3e48cf793a0b654' }"
 
     input:
     tuple val(meta), path(bam), path(bai), path(fusions)

From 5f4536e447c8edaad6708c3209e8936aa9ca3ec4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@scilifelab.se>
Date: Thu, 10 Jul 2025 15:35:55 +0000
Subject: [PATCH 590/887] update test

---
 tests/test_build.nf.test      |   3 +-
 tests/test_build.nf.test.snap | 722 +++++++++++++++++++++++++++++++++-
 2 files changed, 720 insertions(+), 5 deletions(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 72f62ee3f..f12e0e32f 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -3,6 +3,7 @@ nextflow_pipeline {
     name "Test pipeline"
     script "../main.nf"
     profile "test_build"
+    options "-stub"
     tag "pipeline"
     tag "pipeline_rnafusion"
     tag "test_build"
@@ -15,7 +16,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
-                tools            = "ctatsplicing,starfusion,arriba,fusionreport,salmon"
+                tools            = "all"
                 no_cosmic        = true
             }
         }
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 9af3fcc7d..38efc76ca 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -1,33 +1,747 @@
 {
     "Test build references": {
         "content": [
-            1,
+            35,
             {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.1"
+                },
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.5.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.5.0"
+                },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.5.0"
+                },
+                "CTATSPLICING_PREPGENOMELIB": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "CTATSPLICING_STARTOCANCERINTRONS": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "FASTP": {
+                    "fastp": "0.24.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER_DOWNLOAD": {
+                    "wget": "1.21.4",
+                    "tar": 1.35
+                },
+                "FUSIONCATCHER_FUSIONCATCHER": {
+                    "fusioncatcher": "1.33"
+                },
+                "FUSIONINSPECTOR": {
+                    "STAR-Fusion": null
+                },
+                "FUSIONREPORT": {
+                    "fusion_report": "4.0.0"
+                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "4.0.0"
                 },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.15.0"
+                },
+                "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
+                    "STAR-Fusion": "1.15.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/test.tsv",
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "arriba_visualisation",
+                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
+                "ctatsplicing",
+                "ctatsplicing/test.cancer.introns",
+                "ctatsplicing/test.cancer.introns.prelim",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/test.chckpts",
+                "ctatsplicing/test.ctat-splicing.igv.html",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/test.igv.tracks",
+                "ctatsplicing/test.introns",
+                "ctatsplicing/test.introns.for_IGV.bed",
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test.html",
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+                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cancer_splicing.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "refGene.sort.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "refGene.sort.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test_meta_info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.Aligned.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "stringtie.merged.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                {
+                    "directory": false,
+                    "file": true,
+                    "freeSpace": 1219570667520,
+                    "canonicalPath": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
+                    "usableSpace": 1219570667520,
+                    "hidden": false,
+                    "totalSpace": 1288479703040,
+                    "path": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
+                    "name": "test_fusion_data.vcf.gz",
+                    "absolute": true,
+                    "absolutePath": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
+                    "parent": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf"
+                }
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-09T14:55:30.278624"
+        "timestamp": "2025-07-10T15:33:41.470029785"
     }
 }
\ No newline at end of file

From cae84c3dafdac13d9d7908365311ba04ac960a2e Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@scilifelab.se>
Date: Fri, 11 Jul 2025 15:02:38 +0000
Subject: [PATCH 591/887] update test

---
 tests/test_build.nf.test.snap    | 6 +++---
 tests/test_stub_bam.nf.test.snap | 8 ++++----
 2 files changed, 7 insertions(+), 7 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 38efc76ca..75c8e2527 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -725,9 +725,9 @@
                 {
                     "directory": false,
                     "file": true,
-                    "freeSpace": 1219570667520,
+                    "freeSpace": 1152950960128,
                     "canonicalPath": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
-                    "usableSpace": 1219570667520,
+                    "usableSpace": 1152950960128,
                     "hidden": false,
                     "totalSpace": 1288479703040,
                     "path": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
@@ -742,6 +742,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-10T15:33:41.470029785"
+        "timestamp": "2025-07-11T14:39:01.707689765"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 4edb078f8..c5bbb1354 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -430,7 +430,7 @@
                     "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -775,7 +775,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T13:15:34.702165"
+        "timestamp": "2025-07-11T14:56:00.138311656"
     },
     "stub test salmon": {
         "content": [
@@ -1527,7 +1527,7 @@
                     "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
@@ -1976,6 +1976,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T12:55:23.941369"
+        "timestamp": "2025-07-11T14:43:32.816080551"
     }
 }
\ No newline at end of file

From e4a45d9198c7657721ff3993565b0743dbc36e3a Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@scilifelab.se>
Date: Fri, 11 Jul 2025 15:18:52 +0000
Subject: [PATCH 592/887] update snaps

---
 tests/test_stub.nf.test.snap | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 324da36e0..6d1f04f20 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -508,7 +508,7 @@
                     "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -877,7 +877,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T11:48:14.166006"
+        "timestamp": "2025-07-11T15:18:18.890499282"
     },
     "stub test salmon": {
         "content": [
@@ -1705,7 +1705,7 @@
                     "arriba_download": "2.5.0"
                 },
                 "ARRIBA_VISUALISATION": {
-                    "arriba": "2.4.0"
+                    "arriba": "2.5.0"
                 },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
@@ -2178,6 +2178,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T11:29:35.207225"
+        "timestamp": "2025-07-11T15:05:16.899145218"
     }
 }
\ No newline at end of file

From cb08a1e47e5d68e45240d78468e139b133eeb452 Mon Sep 17 00:00:00 2001
From: Annick Renevey <annick.renevey@scilifelab.se>
Date: Fri, 11 Jul 2025 17:16:40 +0000
Subject: [PATCH 593/887] update snap

---
 tests/test_build.nf.test      | 1 -
 tests/test_build.nf.test.snap | 6 +++---
 2 files changed, 3 insertions(+), 4 deletions(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index f12e0e32f..04ca91a05 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -42,5 +42,4 @@ nextflow_pipeline {
             )
         }
     }
-
 }
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 75c8e2527..db471a22a 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -725,9 +725,9 @@
                 {
                     "directory": false,
                     "file": true,
-                    "freeSpace": 1152950960128,
+                    "freeSpace": 1232851841024,
                     "canonicalPath": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
-                    "usableSpace": 1152950960128,
+                    "usableSpace": 1232851841024,
                     "hidden": false,
                     "totalSpace": 1288479703040,
                     "path": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
@@ -742,6 +742,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-11T14:39:01.707689765"
+        "timestamp": "2025-07-11T17:06:58.135785436"
     }
 }
\ No newline at end of file

From e7dfac6c5db84352b98cff804afc5ec8f98dda5b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 09:57:56 +0200
Subject: [PATCH 594/887] update fusioncatcher

---
 modules/local/fusioncatcher/download/main.nf | 6 ++----
 nextflow.config                              | 2 +-
 nextflow_schema.json                         | 1 -
 3 files changed, 3 insertions(+), 6 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index f2cc2a3d5..2cc34f052 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,10 +22,8 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    wget --no-check-certificate $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partaa
-    wget --no-check-certificate $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partab
-    wget --no-check-certificate $args ${params.fusioncatcher_download_link}/human_v${genome_gencode_version}.tar.gz.partac
-    cat human_v${genome_gencode_version}.tar.gz.* | tar xz
+    wget --no-check-certificate $args ${params.fusioncatcher_download_link}
+    tar xz human_v${genome_gencode_version}.tar.gz
     rm human_v${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/nextflow.config b/nextflow.config
index ed615a07e..60e9b8c21 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -64,7 +64,7 @@ params {
 
     // Download references option
     download_refs               = false
-    fusioncatcher_download_link = "https://master.dl.sourceforge.net/project/rnafusion/fusioncatcher"
+    fusioncatcher_download_link = "https://drive.google.com/file/d/1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo/view?usp=drive_link"
 
     // Path to references
     fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index a472d333f..7930bc9fc 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -161,7 +161,6 @@
                     "description": "Fusioncatcher download link",
                     "fa_icon": "far fa-file-code",
                     "pattern": "^https?://.*$",
-                    "default": "https://master.dl.sourceforge.net/project/rnafusion/fusioncatcher"
                 },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",

From a0318eec442477551369e55c3ca7aaea7c201456 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 11:19:26 +0200
Subject: [PATCH 595/887] update tests by removing gz

---
 modules/local/vcf_collect/main.nf       |  5 ++---
 modules/nf-core/arriba/download/main.nf | 10 ++++++----
 nextflow.config                         |  4 ++--
 nextflow_schema.json                    |  6 +++---
 tests/test_build.nf.test.snap           | 25 +++++++------------------
 5 files changed, 20 insertions(+), 30 deletions(-)

diff --git a/modules/local/vcf_collect/main.nf b/modules/local/vcf_collect/main.nf
index 5c1c4ce0e..3f218b66b 100644
--- a/modules/local/vcf_collect/main.nf
+++ b/modules/local/vcf_collect/main.nf
@@ -14,7 +14,7 @@ process VCF_COLLECT {
 
     output:
     path "versions.yml"              , emit: versions
-    tuple val(meta), path("*vcf.gz") , emit: vcf
+    tuple val(meta), path("*vcf")    , emit: vcf
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,7 +24,6 @@ process VCF_COLLECT {
     // TODO use BGZIP to compress the VCF file instead of GZIP
     """
     vcf_collect.py --fusioninspector $fusioninspector_tsv --fusionreport $fusionreport_report --fusioninspector_gtf $fusioninspector_gtf_tsv --fusionreport_csv $fusionreport_csv --hgnc $hgnc_ref --sample ${prefix} --out ${prefix}_fusion_data.vcf
-    gzip ${prefix}_fusion_data.vcf
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -36,7 +35,7 @@ process VCF_COLLECT {
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    touch ${prefix}_fusion_data.vcf.gz
+    touch ${prefix}_fusion_data.vcf
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index fdd46d2e8..444110884 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -11,10 +11,10 @@ process ARRIBA_DOWNLOAD {
     val(genome)
 
     output:
-    path "blacklist*${genome}*.tsv.gz"    , emit: blacklist
+    path "blacklist*${genome}*.tsv"    , emit: blacklist
     path "cytobands*${genome}*.tsv"       , emit: cytobands
     path "protein_domains*${genome}*.gff3", emit: protein_domains
-    path "known_fusions*${genome}*.tsv.gz", emit: known_fusions
+    path "known_fusions*${genome}*.tsv", emit: known_fusions
     path "versions.yml"                   , emit: versions
 
     when:
@@ -27,6 +27,8 @@ process ARRIBA_DOWNLOAD {
     tar -xzvf arriba_v${arriba_version}.tar.gz
     rm arriba_v${arriba_version}.tar.gz
     mv arriba_v${arriba_version}/database/* .
+    tar -xzvf blacklist_hg38_GRCh38_v${arriba_version}.tsv.gz blacklist_hg38_GRCh38_v${arriba_version}.tsv
+    tar -xzvf known_fusions_hg38_GRCh38_v${arriba_version}.tsv.gz known_fusions_hg38_GRCh38_v${arriba_version}.tsv
     rm -r arriba_v${arriba_version}
 
     cat <<-END_VERSIONS > versions.yml
@@ -38,10 +40,10 @@ process ARRIBA_DOWNLOAD {
     stub:
     def arriba_version = '2.5.0'
     """
-    touch blacklist_hg38_GRCh38_v${arriba_version}.tsv.gz
+    touch blacklist_hg38_GRCh38_v${arriba_version}.tsv
     touch protein_domains_hg38_GRCh38_v${arriba_version}.gff3
     touch cytobands_hg38_GRCh38_v${arriba_version}.tsv
-    touch known_fusions_hg38_GRCh38_v${arriba_version}.tsv.gz
+    touch known_fusions_hg38_GRCh38_v${arriba_version}.tsv
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/nextflow.config b/nextflow.config
index 60e9b8c21..f747a9116 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -73,9 +73,9 @@ params {
     refflat                       = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf.refflat"
     rrna_intervals                = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.interval_list"
     no_cosmic                     = false
-    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv"
     arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
-    arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv"
     arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}"
     hgnc_ref                      = "${params.genomes_base}/hgnc/hgnc_complete_set.txt"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 7930bc9fc..a0794cd62 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -97,7 +97,7 @@
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "format": "file-path",
-                    "pattern": "\\S+\\.tsv\\.gz$",
+                    "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
                 },
@@ -111,7 +111,7 @@
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "format": "file-path",
-                    "pattern": "\\S+\\.tsv\\.gz$",
+                    "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference known fusions"
                 },
@@ -160,7 +160,7 @@
                     "type": "string",
                     "description": "Fusioncatcher download link",
                     "fa_icon": "far fa-file-code",
-                    "pattern": "^https?://.*$",
+                    "pattern": "^https?://.*$"
                 },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index db471a22a..ccb650d62 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -297,9 +297,9 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv",
                 "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv",
                 "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
@@ -455,7 +455,7 @@
                 "stringtie/test.gene.abundance.txt",
                 "stringtie/test.transcripts.gtf",
                 "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "vcf/test_fusion_data.vcf"
             ],
             [
                 "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -617,7 +617,9 @@
                 "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test_collectinsertsize.pdf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test_collectinsertsize.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blacklist_hg38_GRCh38_v2.5.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "known_fusions_hg38_GRCh38_v2.5.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -722,26 +724,13 @@
                 "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                {
-                    "directory": false,
-                    "file": true,
-                    "freeSpace": 1232851841024,
-                    "canonicalPath": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
-                    "usableSpace": 1232851841024,
-                    "hidden": false,
-                    "totalSpace": 1288479703040,
-                    "path": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
-                    "name": "test_fusion_data.vcf.gz",
-                    "absolute": true,
-                    "absolutePath": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf/test_fusion_data.vcf.gz",
-                    "parent": "/workspace/rnafusion/.nf-test/tests/c2df4400e7ec2c7cf284bfa4ce0ed5c2/output/vcf"
-                }
+                "test_fusion_data.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-11T17:06:58.135785436"
+        "timestamp": "2025-07-14T11:19:02.774708"
     }
 }
\ No newline at end of file

From 1934f53fd2b41a9adb5399be9ccae0073d077ed2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 13:07:54 +0200
Subject: [PATCH 596/887] update arriba

---
 modules.json                                  |  2 +-
 modules/nf-core/arriba/download/main.nf       | 10 ++-
 modules/nf-core/arriba/download/meta.yml      | 69 ++++++++++---------
 .../arriba/download/tests/main.nf.test.snap   |  2 +-
 4 files changed, 44 insertions(+), 39 deletions(-)

diff --git a/modules.json b/modules.json
index 47d139606..d994a704c 100644
--- a/modules.json
+++ b/modules.json
@@ -17,7 +17,7 @@
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "75771aaffe150962320e3c8c9a025268843fcc46",
+                        "git_sha": "98bd7be6d2191b0d5900ee698d91b52eeab90311",
                         "installed_by": ["modules"]
                     },
                     "cat/cat": {
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index 444110884..baaf72f24 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -11,10 +11,10 @@ process ARRIBA_DOWNLOAD {
     val(genome)
 
     output:
-    path "blacklist*${genome}*.tsv"    , emit: blacklist
+    path "blacklist*${genome}*.tsv.gz"    , emit: blacklist
     path "cytobands*${genome}*.tsv"       , emit: cytobands
     path "protein_domains*${genome}*.gff3", emit: protein_domains
-    path "known_fusions*${genome}*.tsv", emit: known_fusions
+    path "known_fusions*${genome}*.tsv.gz", emit: known_fusions
     path "versions.yml"                   , emit: versions
 
     when:
@@ -27,8 +27,6 @@ process ARRIBA_DOWNLOAD {
     tar -xzvf arriba_v${arriba_version}.tar.gz
     rm arriba_v${arriba_version}.tar.gz
     mv arriba_v${arriba_version}/database/* .
-    tar -xzvf blacklist_hg38_GRCh38_v${arriba_version}.tsv.gz blacklist_hg38_GRCh38_v${arriba_version}.tsv
-    tar -xzvf known_fusions_hg38_GRCh38_v${arriba_version}.tsv.gz known_fusions_hg38_GRCh38_v${arriba_version}.tsv
     rm -r arriba_v${arriba_version}
 
     cat <<-END_VERSIONS > versions.yml
@@ -40,10 +38,10 @@ process ARRIBA_DOWNLOAD {
     stub:
     def arriba_version = '2.5.0'
     """
-    touch blacklist_hg38_GRCh38_v${arriba_version}.tsv
+    echo | gzip > blacklist_hg38_GRCh38_v${arriba_version}.tsv.gz
     touch protein_domains_hg38_GRCh38_v${arriba_version}.gff3
     touch cytobands_hg38_GRCh38_v${arriba_version}.tsv
-    touch known_fusions_hg38_GRCh38_v${arriba_version}.tsv
+    echo | gzip > known_fusions_hg38_GRCh38_v${arriba_version}.tsv.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
index d9e28cc06..06a8ef60d 100644
--- a/modules/nf-core/arriba/download/meta.yml
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -16,37 +16,44 @@ tools:
       identifier: biotools:Arriba
 
 input:
-  - - genome:
-        type: string
-        description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
+  - genome:
+      type: string
+      description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
 output:
-  - blacklist:
-      - blacklist*${genome}*.tsv.gz:
-          type: string
-          description: The blacklist removes recurrent alignment artifacts and transcripts
-            which are present in healthy tissue
-          pattern: ".tsv.gz"
-  - cytobands:
-      - cytobands*${genome}*.tsv:
-          type: file
-          description: Coordinates of the Giemsa staining bands. This information is used
-            to draw ideograms
-          pattern: ".tsv"
-  - protein_domains:
-      - protein_domains*${genome}*.gff3:
-          type: file
-          description: Protein domain annotations
-          pattern: "*.gff3"
-  - known_fusions:
-      - known_fusions*${genome}*.tsv.gz:
-          type: file
-          description: Arriba is more sensitive to those fusions to improve the detection
-            rate of expected or highly relevant events, such as recurrent fusions
-          pattern: "*.tsv.gz"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+  blacklist:
+    - blacklist*${genome}*.tsv.gz:
+        type: string
+        description: The blacklist removes recurrent alignment artifacts and transcripts
+          which are present in healthy tissue
+        pattern: ".tsv.gz"
+  cytobands:
+    - cytobands*${genome}*.tsv:
+        type: file
+        description: Coordinates of the Giemsa staining bands. This information is used
+          to draw ideograms
+        pattern: ".tsv"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+  protein_domains:
+    - protein_domains*${genome}*.gff3:
+        type: file
+        description: Protein domain annotations
+        pattern: "*.gff3"
+        ontologies: []
+  known_fusions:
+    - known_fusions*${genome}*.tsv.gz:
+        type: file
+        description: Arriba is more sensitive to those fusions to improve the detection
+          rate of expected or highly relevant events, such as recurrent fusions
+        pattern: "*.tsv.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@peterpru"
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
index 87e41a84b..ec25745a7 100644
--- a/modules/nf-core/arriba/download/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/download/tests/main.nf.test.snap
@@ -38,6 +38,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-04T12:36:28.499552"
+        "timestamp": "2025-07-14T11:54:10.203072"
     }
 }
\ No newline at end of file

From 74cc144e3ddc493cd63871c1ebd0eb632d53ff38 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 13:22:50 +0200
Subject: [PATCH 597/887] update snap

---
 tests/test_build.nf.test.snap | 8 +++-----
 1 file changed, 3 insertions(+), 5 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index ccb650d62..f8d21222f 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -297,9 +297,9 @@
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
                 "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
                 "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
                 "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
@@ -617,9 +617,7 @@
                 "test.rna_metrics:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test_collectinsertsize.pdf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test_collectinsertsize.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blacklist_hg38_GRCh38_v2.5.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "known_fusions_hg38_GRCh38_v2.5.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -731,6 +729,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T11:19:02.774708"
+        "timestamp": "2025-07-14T13:11:06.709587"
     }
 }
\ No newline at end of file

From e8ea7b3c20131bfab238e8fa3a6d11dfbaafde03 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 14 Jul 2025 12:33:22 +0000
Subject: [PATCH 598/887] Reintegrate starfusion/build from nf-core

---
 modules.json                                  |   5 +
 .../prepgenomelib/tests/main.nf.test          |   2 +-
 .../local/fusioninspector/tests/main.nf.test  |   2 +-
 modules/local/starfusion/build/main.nf        | 143 -------------
 modules/local/starfusion/build/meta.yml       |  44 ----
 .../local/starfusion/build/tests/main.nf.test | 142 -------------
 .../starfusion/detect/tests/main.nf.test      |  23 ++-
 .../starfusion/build/environment.yml          |   0
 modules/nf-core/starfusion/build/main.nf      | 148 ++++++++++++++
 modules/nf-core/starfusion/build/meta.yml     |  74 +++++++
 .../starfusion/build/tests/main.nf.test       | 188 ++++++++++++++++++
 .../starfusion/build/tests/main.nf.test.snap  | 124 ++++++------
 subworkflows/local/build_references/main.nf   |   2 +-
 .../starfusion_workflow/tests/main.nf.test    |   2 +-
 14 files changed, 497 insertions(+), 402 deletions(-)
 delete mode 100644 modules/local/starfusion/build/main.nf
 delete mode 100644 modules/local/starfusion/build/meta.yml
 delete mode 100644 modules/local/starfusion/build/tests/main.nf.test
 rename modules/{local => nf-core}/starfusion/build/environment.yml (100%)
 create mode 100644 modules/nf-core/starfusion/build/main.nf
 create mode 100644 modules/nf-core/starfusion/build/meta.yml
 create mode 100644 modules/nf-core/starfusion/build/tests/main.nf.test
 rename modules/{local => nf-core}/starfusion/build/tests/main.nf.test.snap (81%)

diff --git a/modules.json b/modules.json
index b7a41b4df..455bd01b3 100644
--- a/modules.json
+++ b/modules.json
@@ -135,6 +135,11 @@
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["modules"]
                     },
+                    "starfusion/build": {
+                        "branch": "master",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "installed_by": ["modules"]
+                    },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
index de69cd3e2..2d694202a 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -6,7 +6,7 @@ nextflow_process {
 
     setup {
         run("STARFUSION_BUILD") {
-            script "../../../starfusion/build/main.nf"
+            script "../../../../nf-core/starfusion/build/main.nf"
             process {
                 """
                 input[0] = [
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 65a190789..8868b847b 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -12,7 +12,7 @@ nextflow_process {
 
         setup {
             run("STARFUSION_BUILD") {
-                script "../../starfusion/build/main.nf"
+                script "../../../../nf-core/starfusion/build/main.nf"
                 process {
                     """
                     input[0] = [
diff --git a/modules/local/starfusion/build/main.nf b/modules/local/starfusion/build/main.nf
deleted file mode 100644
index 1f49df237..000000000
--- a/modules/local/starfusion/build/main.nf
+++ /dev/null
@@ -1,143 +0,0 @@
-process STARFUSION_BUILD {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/75/75d085bf2a8e40c6693b357800eef0f9568f661226d0888339bc77f7852234bb/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:e285bb3eb373b9a7'}"
-
-    input:
-    tuple val(meta), path(fasta)
-    tuple val(meta2), path(gtf)
-    path fusion_annot_lib
-    val dfam_species
-    val dfam_version
-    val pfam_version
-
-    output:
-    tuple val(meta), path("ctat_genome_lib_build_dir"), emit: reference
-    path "versions.yml"   , emit: versions
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    wget http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${pfam_version}/Pfam-A.hmm.gz --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3f --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3i --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3m --no-check-certificate
-    wget https://www.dfam.org/releases/Dfam_${dfam_version}/infrastructure/dfamscan/${dfam_species}_dfam.hmm.h3p --no-check-certificate
-    gunzip Pfam-A.hmm.gz && hmmpress Pfam-A.hmm
-    wget https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm -O AnnotFilterRule.pm --no-check-certificate
-
-    prep_genome_lib.pl \\
-        --genome_fa $fasta \\
-        --gtf $gtf \\
-        --dfam_db ${dfam_species}_dfam.hmm \\
-        --pfam_db Pfam-A.hmm \\
-        --fusion_annot_lib $fusion_annot_lib \\
-        --annot_filter_rule AnnotFilterRule.pm \\
-        --CPU $task.cpus \\
-        ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
-        STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    mkdir -p ctat_genome_lib_build_dir
-
-    touch ctat_genome_lib_build_dir/AnnotFilterRule.pm
-    gzip -c /dev/null > ctat_genome_lib_build_dir/blast_pairs.dat.gz
-    touch ctat_genome_lib_build_dir/blast_pairs.idx
-
-    mkdir -p ctat_genome_lib_build_dir/__chkpts
-    touch ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok
-    touch ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok
-    touch ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok
-    touch ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok
-    touch ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok
-    touch ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok
-    touch ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok
-    touch ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok
-    touch ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok
-    touch ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok
-    touch ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok
-    touch ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok
-    touch ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok
-    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok
-    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok
-    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok
-    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok
-    touch ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok
-    touch ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok
-    touch ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok
-    touch ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok
-    touch ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok
-    touch ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok
-
-    gzip -c /dev/null > ctat_genome_lib_build_dir/fusion_annot_lib.gz
-    touch ctat_genome_lib_build_dir/fusion_annot_lib.idx
-    touch ctat_genome_lib_build_dir/pfam_domains.dbm
-    gzip -c /dev/null > ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
-
-    touch ctat_genome_lib_build_dir/ref_annot.cdna.fa
-    touch ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx
-    touch ctat_genome_lib_build_dir/ref_annot.cds
-    touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
-    touch ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
-    touch ctat_genome_lib_build_dir/ref_annot.gtf
-    touch ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
-    touch ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-    touch ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed
-    touch ctat_genome_lib_build_dir/ref_annot.pep
-    touch ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
-
-    touch ctat_genome_lib_build_dir/ref_genome.fa
-    touch ctat_genome_lib_build_dir/ref_genome.fa.fai
-    touch ctat_genome_lib_build_dir/ref_genome.fa.mm2
-    touch ctat_genome_lib_build_dir/ref_genome.fa.ndb
-    touch ctat_genome_lib_build_dir/ref_genome.fa.nhr
-    touch ctat_genome_lib_build_dir/ref_genome.fa.nin
-    touch ctat_genome_lib_build_dir/ref_genome.fa.njs
-    touch ctat_genome_lib_build_dir/ref_genome.fa.not
-    touch ctat_genome_lib_build_dir/ref_genome.fa.nsq
-    touch ctat_genome_lib_build_dir/ref_genome.fa.ntf
-    touch ctat_genome_lib_build_dir/ref_genome.fa.nto
-
-    mkdir -p ctat_genome_lib_build_dir/ref_genome.fa.star.idx
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab
-    touch ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab
-
-    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat
-    touch ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
-    gzip -c /dev/null > ctat_genome_lib_build_dir/trans.blast.dat.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
-        STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
-    END_VERSIONS
-    """
-
-}
diff --git a/modules/local/starfusion/build/meta.yml b/modules/local/starfusion/build/meta.yml
deleted file mode 100644
index bfa0a025a..000000000
--- a/modules/local/starfusion/build/meta.yml
+++ /dev/null
@@ -1,44 +0,0 @@
-name: starfusion_build
-description: Download STAR-fusion genome resource required to run STAR-Fusion caller
-keywords:
-  - download
-tools:
-  - star-fusion:
-      description: Fusion calling algorithm for RNAseq data
-      homepage: https://github.com/STAR-Fusion/
-      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
-      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
-      doi: "10.1186/s13059-019-1842-9"
-      licence: ["GPL v3"]
-
-input:
-  - fasta:
-      type: file
-      description: genome fasta file
-      pattern: "*.{fasta}"
-  - gtf:
-      type: file
-      description: genome gtf file
-      pattern: "*.{gtf}"
-  - fusion_annot_lib:
-      type: file
-      description: Fusion annotation library (key/val pairs, tab-delimited).
-      pattern: "*.dat.gz"
-  - dfam_species:
-      type: string
-      description: DNA transposable element database (Dfam.hmm), required for repeat masking. Names such as 'homo_sapiens' are accepted (will automatically pull the resources from dfam).
-  - dfam_version:
-      type: string
-      description: DNA transposable element database version, required for repeat masking. Values such as '3.4' are accepted (will automatically pull the resources from dfam).
-  - pfam_version:
-      type: string
-      description: Protein families database version, to get domain information. Values such as '37.4' are accepted (will automatically pull the resources from Pfam).
-
-output:
-  - reference:
-      type: directory
-      description: Reference dir
-      pattern: "ctat_genome_lib_build_dir"
-
-authors:
-  - "@praveenraj2018"
diff --git a/modules/local/starfusion/build/tests/main.nf.test b/modules/local/starfusion/build/tests/main.nf.test
deleted file mode 100644
index 07777230e..000000000
--- a/modules/local/starfusion/build/tests/main.nf.test
+++ /dev/null
@@ -1,142 +0,0 @@
-nextflow_process {
-
-    name "Test Process STARFUSION_BUILD"
-    script "../main.nf"
-    process "STARFUSION_BUILD"
-
-    test("STARFUSION_BUILD - human - minigenome") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
-                ]
-                input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
-                ]
-
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "homo_sapiens"
-                input [4] = "3.8"
-                input [5] = "37.4"
-
-                """
-            }
-        }
-
-        then {
-            assert snapshot(
-                    path(process.out.reference[0][1]).resolve("AnnotFilterRule.pm"),
-                    path(process.out.reference[0][1]).resolve("blast_pairs.dat.gz").exists(),
-                    path(process.out.reference[0][1]).resolve("blast_pairs.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("__chkpts/annotfiltrule_cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/blast_pairs.idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/cp_gene_blast_pairs.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/cp_pfam_dat.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/cp_ref_annot_cdna.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/fusion_annot_lib.cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/_fusion_annot_lib.idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/index_pfam_hits.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/index_ref_annot_cdna.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/makeblastdb.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/mm2_genome_idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/mm2.splice_bed.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/_prot_info_db.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.gene_spans.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.mini.sortu.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome_fai.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome.fa.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.index.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/validate_ctat_genome_lib.ok"),
-                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.gz"),
-                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("pfam_domains.dbm").exists(),
-                    path(process.out.reference[0][1]).resolve("PFAM.domtblout.dat.gz").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cds").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf"),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.gene_spans").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mini.sortu"),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mm2.splice.bed"),
-                    path(process.out.reference[0][1]).resolve("ref_annot.pep").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.prot_info.dbm").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.fai"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.mm2"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ndb"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nhr"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nin").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.njs").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.not"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nsq"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ntf"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nto"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/build.ok"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrLength.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrNameLength.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrName.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrStart.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonGeTrInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/geneInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Genome"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/genomeParameters.txt").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Log.out").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/transcriptInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
-                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dbm").exists(),
-                    path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
-                    process.out.versions
-                ).match()
-        }
-
-    }
-
-    test("STARFUSION_BUILD - human - minigenome - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
-                ]
-                input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
-                ]
-
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "homo_sapiens"
-                input [4] = "3.8"
-                input [5] = "37.4"
-
-                """
-            }
-        }
-
-        then {
-            assert snapshot(process.out).match()
-        }
-
-    }
-
-}
diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
index 75e0bfdd7..c8bf4e119 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test
+++ b/modules/local/starfusion/detect/tests/main.nf.test
@@ -6,20 +6,29 @@ nextflow_process {
 
     setup {
         run("STARFUSION_BUILD") {
-            script "../../../starfusion/build/main.nf"
+            script "../../../../nf-core/starfusion/build/main.nf"
             process {
                 """
                 input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                    [ id:'minigenome_fasta' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
                 ]
                 input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                    [ id:'minigenome_gtf' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
                 ]
 
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                input[3] = "homo_sapiens"
+                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[5] = [
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                ]
+                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                 """
             }
         }
diff --git a/modules/local/starfusion/build/environment.yml b/modules/nf-core/starfusion/build/environment.yml
similarity index 100%
rename from modules/local/starfusion/build/environment.yml
rename to modules/nf-core/starfusion/build/environment.yml
diff --git a/modules/nf-core/starfusion/build/main.nf b/modules/nf-core/starfusion/build/main.nf
new file mode 100644
index 000000000..33882c082
--- /dev/null
+++ b/modules/nf-core/starfusion/build/main.nf
@@ -0,0 +1,148 @@
+process STARFUSION_BUILD {
+    tag "$meta.id"
+    label 'process_high'
+    stageInMode 'copy'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/75/75d085bf2a8e40c6693b357800eef0f9568f661226d0888339bc77f7852234bb/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:e285bb3eb373b9a7'}"
+
+    input:
+    tuple val(meta), path(fasta)
+    tuple val(meta2), path(gtf)
+    path fusion_annot_lib
+    val dfam_species
+    path pfam_url
+    path dfam_urls, arity: '5'
+    path annot_filter_url
+
+    output:
+    tuple val(meta), path("${prefix}_genome_lib_build_dir"), emit: reference
+    path "versions.yml", emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '1.15.1' // WARN: This is the actual version of the STAR-FUSION, but version information of tool is not updated and prints '1.15.0'
+    """
+    gunzip ${pfam_url} && hmmpress Pfam-A.hmm
+
+    prep_genome_lib.pl \\
+        --genome_fa $fasta \\
+        --gtf $gtf \\
+        --dfam_db *_dfam.hmm \\
+        --pfam_db Pfam-A.hmm \\
+        --fusion_annot_lib $fusion_annot_lib \\
+        --annot_filter_rule ${annot_filter_url} \\
+        --CPU $task.cpus \\
+        --output_dir ${prefix}_genome_lib_build_dir \\
+        ${args}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
+        hmmer: \$(echo \$(hmmpress -h | grep HMMER | sed 's/# HMMER //' | sed 's/ .*//' 2>&1))
+        STAR-Fusion: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '1.15.1' // WARN: This is the actual version of the STAR-FUSION, but version information of tool is not updated and prints '1.15.0'
+    """
+    mkdir -p ${prefix}_genome_lib_build_dir
+
+    touch ${prefix}_genome_lib_build_dir/AnnotFilterRule.pm
+    echo | gzip > ${prefix}_genome_lib_build_dir/blast_pairs.dat.gz
+    touch ${prefix}_genome_lib_build_dir/blast_pairs.idx
+
+    mkdir -p ${prefix}_genome_lib_build_dir/__chkpts
+    touch ${prefix}_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/index_pfam_hits.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/makeblastdb.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/_prot_info_db.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/ref_genome_fai.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/ref_genome.fa.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok
+    touch ${prefix}_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok
+
+    echo | gzip > ${prefix}_genome_lib_build_dir/fusion_annot_lib.gz
+    touch ${prefix}_genome_lib_build_dir/fusion_annot_lib.idx
+    touch ${prefix}_genome_lib_build_dir/pfam_domains.dbm
+    echo | gzip > ${prefix}_genome_lib_build_dir/PFAM.domtblout.dat.gz
+
+    touch ${prefix}_genome_lib_build_dir/ref_annot.cdna.fa
+    touch ${prefix}_genome_lib_build_dir/ref_annot.cdna.fa.idx
+    touch ${prefix}_genome_lib_build_dir/ref_annot.cds
+    touch ${prefix}_genome_lib_build_dir/ref_annot.cdsplus.fa
+    touch ${prefix}_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
+    touch ${prefix}_genome_lib_build_dir/ref_annot.gtf
+    touch ${prefix}_genome_lib_build_dir/ref_annot.gtf.gene_spans
+    touch ${prefix}_genome_lib_build_dir/ref_annot.gtf.mini.sortu
+    touch ${prefix}_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed
+    touch ${prefix}_genome_lib_build_dir/ref_annot.pep
+    touch ${prefix}_genome_lib_build_dir/ref_annot.prot_info.dbm
+
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.fai
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.mm2
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.ndb
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.nhr
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.nin
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.njs
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.not
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.nsq
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.ntf
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.nto
+
+    mkdir -p ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/Genome
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/SA
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab
+    touch ${prefix}_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab
+
+    touch ${prefix}_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat
+    touch ${prefix}_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
+    echo | gzip > ${prefix}_genome_lib_build_dir/trans.blast.dat.gz
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
+        hmmer: \$(echo \$(hmmpress -h | grep HMMER | sed 's/# HMMER //' | sed 's/ .*//' 2>&1))
+        STAR-Fusion: $VERSION
+    END_VERSIONS
+    """
+
+}
diff --git a/modules/nf-core/starfusion/build/meta.yml b/modules/nf-core/starfusion/build/meta.yml
new file mode 100644
index 000000000..c7c98bf1e
--- /dev/null
+++ b/modules/nf-core/starfusion/build/meta.yml
@@ -0,0 +1,74 @@
+name: starfusion_build
+description: Download STAR-fusion genome resource required to run STAR-Fusion caller
+keywords:
+  - download
+  - starfusion
+  - build
+
+tools:
+  - star-fusion:
+      description: Fusion calling algorithm for RNAseq data
+      homepage: https://github.com/STAR-Fusion/
+      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki/installing-star-fusion
+      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion
+      doi: "10.1186/s13059-019-1842-9"
+      licence: ["GPL v3"]
+      identifier: ""
+
+input:
+  - - meta:
+        type: map
+        description: Metadata map
+        required: true
+    - fasta:
+        type: file
+        description: Input FASTA file
+        pattern: "*.{fa,fasta}"
+        required: true
+        ontologies:
+          - edam: http://edamontology.org/format_1929 # FASTA
+  - - meta2:
+        type: map
+        description: Second metadata map
+        required: true
+    - gtf:
+        type: file
+        description: Input GTF (Gene Transfer Format) file
+        pattern: "*.gtf"
+        required: true
+        ontologies:
+          - edam: "http://edamontology.org/format_2306" # GTF
+  - fusion_annot_lib:
+      type: file
+      description: Fusion annotation library file containing known fusion genes
+      required: true
+      ontologies:
+        - edam: "http://edamontology.org/topic_0203" # Gene fusion
+  - dfam_species:
+      type: string
+      description: Dfam species name
+output:
+  reference:
+    - - meta:
+          type: map
+          description: Metadata map
+      - ${prefix}_genome_lib_build_dir:
+          type: directory
+          description: Genome library build directory
+          pattern: "*_genome_lib_build_dir"
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@praveenraj2018"
+  - "@martings"
+  - "@alanmmobbs93"
+  - "@delfiterradas"
+  - "@sofiromano"
+
+maintainers:
+  - "@praveenraj2018"
diff --git a/modules/nf-core/starfusion/build/tests/main.nf.test b/modules/nf-core/starfusion/build/tests/main.nf.test
new file mode 100644
index 000000000..ae18299e2
--- /dev/null
+++ b/modules/nf-core/starfusion/build/tests/main.nf.test
@@ -0,0 +1,188 @@
+nextflow_process {
+    name "Test Process STARFUSION_BUILD"
+    script "../main.nf"
+    process "STARFUSION_BUILD"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "starfusion"
+    tag "starfusion/build"
+    tag "fusioninspector"
+
+    test("STARFUSION_BUILD - human - minigenome") {
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome_fasta' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
+                ]
+                input[1] = [
+                    [ id:'minigenome_gtf' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
+                ]
+
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                input[3] = "homo_sapiens"
+                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[5] = [
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                ]
+                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                """
+            }
+        }
+
+        then {
+            def outputDir = file(process.out.reference[0][1])
+            assert snapshot(
+                path(process.out.reference[0][1]).resolve("AnnotFilterRule.pm"),
+                file("$outputDir/blast_pairs.dat.gz").name,
+                file("$outputDir/blast_pairs.idx").name,
+                file("$outputDir/__chkpts/annotfiltrule_cp.ok").name,
+                file("$outputDir/__chkpts/blast_pairs.idx.ok").name,
+                file("$outputDir/__chkpts/cp_gene_blast_pairs.ok").name,
+                file("$outputDir/__chkpts/cp_pfam_dat.ok").name,
+                file("$outputDir/__chkpts/cp_ref_annot_cdna.ok").name,
+                file("$outputDir/__chkpts/fusion_annot_lib.cp.ok").name,
+                file("$outputDir/__chkpts/_fusion_annot_lib.idx.ok").name,
+                file("$outputDir/__chkpts/index_pfam_hits.ok").name,
+                file("$outputDir/__chkpts/index_ref_annot_cdna.ok").name,
+                file("$outputDir/__chkpts/makeblastdb.ok").name,
+                file("$outputDir/__chkpts/mm2_genome_idx.ok").name,
+                file("$outputDir/__chkpts/mm2.splice_bed.ok").name,
+                file("$outputDir/__chkpts/_prot_info_db.ok").name,
+                file("$outputDir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok").name,
+                file("$outputDir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok").name,
+                file("$outputDir/__chkpts/ref_annot.gtf.gene_spans.ok").name,
+                file("$outputDir/__chkpts/ref_annot.gtf.mini.sortu.ok").name,
+                file("$outputDir/__chkpts/ref_annot.gtf.ok").name,
+                file("$outputDir/__chkpts/ref_genome_fai.ok").name,
+                file("$outputDir/__chkpts/ref_genome.fa.ok").name,
+                file("$outputDir/__chkpts/trans.blast.dat.cp.ok").name,
+                file("$outputDir/__chkpts/trans.blast.dat.index.ok").name,
+                file("$outputDir/__chkpts/validate_ctat_genome_lib.ok").name,
+                path(process.out.reference[0][1]).resolve("fusion_annot_lib.gz"),
+                file("$outputDir/fusion_annot_lib.idx").name,
+                file("$outputDir/pfam_domains.dbm").name,
+                file("$outputDir/PFAM.domtblout.dat.gz").name,
+                file("$outputDir/ref_annot.cdna.fa").name,
+                file("$outputDir/ref_annot.cdna.fa.idx").name,
+                file("$outputDir/ref_annot.cds").name,
+                file("$outputDir/ref_annot.cdsplus.fa").name,
+                file("$outputDir/ref_annot.cdsplus.fa.idx").name,
+                path(process.out.reference[0][1]).resolve("ref_annot.gtf"),
+                file("$outputDir/ref_annot.gtf.gene_spans").name,
+                path(process.out.reference[0][1]).resolve("ref_annot.gtf.mini.sortu"),
+                path(process.out.reference[0][1]).resolve("ref_annot.gtf.mm2.splice.bed"),
+                file("$outputDir/ref_annot.pep").name,
+                file("$outputDir/ref_annot.prot_info.dbm").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.fai"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.mm2"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.ndb"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.nhr"),
+                file("$outputDir/ref_genome.fa.nin").name,
+                file("$outputDir/ref_genome.fa.njs").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.not"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.nsq"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.ntf"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.nto"),
+                file("$outputDir/ref_genome.fa.star.idx").name,
+                file("$outputDir/ref_genome.fa.star.idx/build.ok").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrLength.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrNameLength.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrName.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrStart.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonGeTrInfo.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonInfo.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/geneInfo.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Genome"),
+                file("$outputDir/ref_genome.fa.star.idx/genomeParameters.txt").name,
+                file("$outputDir/ref_genome.fa.star.idx/Log.out").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/transcriptInfo.tab"),
+                path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
+                file("$outputDir/trans.blast.align_coords.align_coords.dbm").name,
+                path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
+                process.out.versions
+            ).match()
+        }
+    }
+
+    test("STARFUSION_BUILD - human - minigenome - stub") {
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome_fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                ]
+                input[1] = [
+                    [ id:'minigenome_gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                ]
+
+                input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input[3] = "homo_sapiens"
+                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[5] = [
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                ]
+                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                """
+            }
+        }
+
+        then {
+            assert snapshot(process.out).match()
+        }
+    }
+
+    test("STARFUSION_BUILD - stub - fail") {
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome_fasta' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                ]
+                input[1] = [
+                    [ id:'minigenome_gtf' ],
+                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                ]
+
+                input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input[3] = "homo_sapiens"
+                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[5] = [
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m"
+                ]
+                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                """
+            }
+        }
+
+        then {
+            assert process.failed
+        }
+    }
+}
diff --git a/modules/local/starfusion/build/tests/main.nf.test.snap b/modules/nf-core/starfusion/build/tests/main.nf.test.snap
similarity index 81%
rename from modules/local/starfusion/build/tests/main.nf.test.snap
rename to modules/nf-core/starfusion/build/tests/main.nf.test.snap
index 3e6358d68..c26afe6ea 100644
--- a/modules/local/starfusion/build/tests/main.nf.test.snap
+++ b/modules/nf-core/starfusion/build/tests/main.nf.test.snap
@@ -2,59 +2,59 @@
     "STARFUSION_BUILD - human - minigenome": {
         "content": [
             "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-            true,
-            true,
-            "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "blast_pairs.dat.gz",
+            "blast_pairs.idx",
+            "annotfiltrule_cp.ok",
+            "blast_pairs.idx.ok",
+            "cp_gene_blast_pairs.ok",
+            "cp_pfam_dat.ok",
+            "cp_ref_annot_cdna.ok",
+            "fusion_annot_lib.cp.ok",
+            "_fusion_annot_lib.idx.ok",
+            "index_pfam_hits.ok",
+            "index_ref_annot_cdna.ok",
+            "makeblastdb.ok",
+            "mm2_genome_idx.ok",
+            "mm2.splice_bed.ok",
+            "_prot_info_db.ok",
+            "ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+            "ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+            "ref_annot.gtf.gene_spans.ok",
+            "ref_annot.gtf.mini.sortu.ok",
+            "ref_annot.gtf.ok",
+            "ref_genome_fai.ok",
+            "ref_genome.fa.ok",
+            "trans.blast.dat.cp.ok",
+            "trans.blast.dat.index.ok",
+            "validate_ctat_genome_lib.ok",
             "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-            true,
-            true,
-            true,
-            true,
-            true,
-            true,
-            true,
-            true,
+            "fusion_annot_lib.idx",
+            "pfam_domains.dbm",
+            "PFAM.domtblout.dat.gz",
+            "ref_annot.cdna.fa",
+            "ref_annot.cdna.fa.idx",
+            "ref_annot.cds",
+            "ref_annot.cdsplus.fa",
+            "ref_annot.cdsplus.fa.idx",
             "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-            true,
+            "ref_annot.gtf.gene_spans",
             "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
             "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-            true,
-            true,
+            "ref_annot.pep",
+            "ref_annot.prot_info.dbm",
             "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
             "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
             "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
             "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
             "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-            true,
-            true,
+            "ref_genome.fa.nin",
+            "ref_genome.fa.njs",
             "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
             "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
             "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
             "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
-            true,
-            "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_genome.fa.star.idx",
+            "build.ok",
             "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
             "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
             "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
@@ -63,8 +63,8 @@
             "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
             "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
             "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-            true,
-            true,
+            "genomeParameters.txt",
+            "Log.out",
             "SA:md5,7dd9083264be9c6a2194d990bc10d237",
             "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
             "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
@@ -72,17 +72,17 @@
             "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
             "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
             "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-            true,
+            "trans.blast.align_coords.align_coords.dbm",
             "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
             [
-                "versions.yml:md5,99533491facb961016dafc1d45a8101e"
+                "versions.yml:md5,8a4a70b89be13a4fae8eac764b62045c"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.5"
         },
-        "timestamp": "2025-05-07T20:31:12.177376155"
+        "timestamp": "2025-07-04T14:34:34.16518489"
     },
     "STARFUSION_BUILD - human - minigenome - stub": {
         "content": [
@@ -90,11 +90,11 @@
                 "0": [
                     [
                         {
-                            "id": "minigenome fasta"
+                            "id": "minigenome_fasta"
                         },
                         [
                             "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "PFAM.domtblout.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             [
                                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -120,9 +120,9 @@
                                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
                             ],
-                            "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -168,21 +168,21 @@
                             ],
                             "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "trans.blast.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,99533491facb961016dafc1d45a8101e"
+                    "versions.yml:md5,8a4a70b89be13a4fae8eac764b62045c"
                 ],
                 "reference": [
                     [
                         {
-                            "id": "minigenome fasta"
+                            "id": "minigenome_fasta"
                         },
                         [
                             "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "PFAM.domtblout.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             [
                                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -208,9 +208,9 @@
                                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
                             ],
-                            "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -256,19 +256,19 @@
                             ],
                             "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "trans.blast.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,99533491facb961016dafc1d45a8101e"
+                    "versions.yml:md5,8a4a70b89be13a4fae8eac764b62045c"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.5"
         },
-        "timestamp": "2025-05-07T20:31:53.437179641"
+        "timestamp": "2025-07-04T14:35:41.16179905"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 5751627ff..4ea4e1500 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -8,7 +8,7 @@ include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode
 include { FUSIONCATCHER_DOWNLOAD }          from '../../../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/nf-core/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
-include { STARFUSION_BUILD }                from '../../../modules/local/starfusion/build/main'
+include { STARFUSION_BUILD }                from '../../../modules/nf-core/starfusion/build/main'
 include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
 include { GET_RRNA_TRANSCRIPTS }            from '../../../modules/local/get_rrna_transcript/main'
 include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatsplicing/prepgenomelib/main.nf'
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
index af6b117ff..34ec9f35a 100644
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test
+++ b/subworkflows/local/starfusion_workflow/tests/main.nf.test
@@ -33,7 +33,7 @@ nextflow_workflow {
             }
 
             run("STARFUSION_BUILD") {
-            script "../../../../modules/local/starfusion/build/main.nf"
+            script "../../../../modules/nf-core/starfusion/build/main.nf"
             process {
                 """
                 input[0] = [

From 6a2d6692a49103708603604a5b5f7084b161165f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 15:09:15 +0200
Subject: [PATCH 599/887] update for nf-lint

---
 modules.json                                  |  2 +-
 .../fusioncatcher/fusioncatcher/meta.yml      | 35 +++++++++++-------
 ro-crate-metadata.json                        | 37 +++++++++++++++----
 3 files changed, 51 insertions(+), 23 deletions(-)

diff --git a/modules.json b/modules.json
index 9ebd9bb20..8aa79248c 100644
--- a/modules.json
+++ b/modules.json
@@ -42,7 +42,7 @@
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
-                        "git_sha": "1037205706929921360f57e8042eae6819825e31",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
index ff6647d65..ae9c37e95 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/meta.yml
@@ -1,14 +1,15 @@
----
 # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
 name: "fusioncatcher_fusioncatcher"
-description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
+description: FusionCatcher searches for novel/known somatic fusion genes, translocations,
+  and chimeras in RNA-seq data
 keywords:
   - fusion
   - rna
   - fastq
 tools:
   - fusioncatcher:
-      description: FusionCatcher searches for novel/known somatic fusion genes, translocations, and chimeras in RNA-seq data
+      description: FusionCatcher searches for novel/known somatic fusion genes, translocations,
+        and chimeras in RNA-seq data
       homepage: https://github.com/ndaniel/fusioncatcher
       documentation: https://github.com/ndaniel/fusioncatcher/wiki
       tool_dev_url: https://github.com/ndaniel/fusioncatcher
@@ -35,9 +36,10 @@ input:
         type: file
         description: Directory containing fusioncatcher reference files
 
+        ontologies: []
 output:
-  - fusions:
-      - meta:
+  fusions:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -46,8 +48,9 @@ output:
           type: file
           description: The detected fusions in the input files
           pattern: "*.fusion-genes.txt"
-  - summary:
-      - meta:
+          ontologies: []
+  summary:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -56,8 +59,9 @@ output:
           type: file
           description: The generated summary
           pattern: "*.summary.txt"
-  - log:
-      - meta:
+          ontologies: []
+  log:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -66,12 +70,15 @@ output:
           type: file
           description: The Fusioncatcher log
           pattern: "*.log"
-  - versions:
-      - "versions.yml":
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
 
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@nvnieuwk"
 maintainers:
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index abbf99167..7b0e6e57b 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### Build references\n\n`--references_only` triggers a workflow to ONLY build references, otherwise the references are build when the analysis is run:\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
@@ -121,7 +121,11 @@
         },
         {
             "@id": "main.nf",
-            "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
+            "@type": [
+                "File",
+                "SoftwareSourceCode",
+                "ComputationalWorkflow"
+            ],
             "creator": [
                 {
                     "@id": "#phil.ewels@scilifelab.se"
@@ -139,22 +143,39 @@
             "dateCreated": "",
             "dateModified": "2025-07-08T11:39:11Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
-            "keywords": ["nf-core", "nextflow", "fusion", "fusion-genes", "gene-fusion", "rna", "rna-seq"],
-            "license": ["MIT"],
+            "keywords": [
+                "nf-core",
+                "nextflow",
+                "fusion",
+                "fusion-genes",
+                "gene-fusion",
+                "rna",
+                "rna-seq"
+            ],
+            "license": [
+                "MIT"
+            ],
             "maintainer": [
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
                 }
             ],
-            "name": ["nf-core/rnafusion"],
+            "name": [
+                "nf-core/rnafusion"
+            ],
             "programmingLanguage": {
                 "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow"
             },
             "sdPublisher": {
                 "@id": "https://nf-co.re/"
             },
-            "url": ["https://github.com/nf-core/rnafusion", "https://nf-co.re/rnafusion/dev/"],
-            "version": ["4.0.0dev"]
+            "url": [
+                "https://github.com/nf-core/rnafusion",
+                "https://nf-co.re/rnafusion/dev/"
+            ],
+            "version": [
+                "4.0.0dev"
+            ]
         },
         {
             "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow",
@@ -335,4 +356,4 @@
             "name": "Rickard Hammar\u00e9n"
         }
     ]
-}
+}
\ No newline at end of file

From 6ee156b14a19c7cfd3773eeacc9a8b1968906983 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 14 Jul 2025 13:21:15 +0000
Subject: [PATCH 600/887] Modify input for newer version of starfusion/build

---
 subworkflows/local/build_references/main.nf | 11 ++++++++++-
 1 file changed, 10 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 4ea4e1500..d3bcf108c 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -171,7 +171,16 @@ workflow BUILD_REFERENCES {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
             }
-            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, params.dfam_version, params.pfam_version)
+            pfam_url     = Channel.of(["http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"])
+            dfam_urls_ch = Channel.of([
+                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm",
+                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f",
+                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i",
+                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m",
+                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p"
+            ])
+            annot_filter = Channel.of(["https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"])
+            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, pfam_url, dfam_urls_ch, annot_filter)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
                 CTATSPLICING_PREPGENOMELIB(

From 99d0d41c0f771cd2ebd686fa0ebe4de8056416fa Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 15:22:47 +0200
Subject: [PATCH 601/887] linting

---
 docs/output.md                                    | 1 -
 modules/local/starfusion/detect/main.nf           | 2 --
 subworkflows/local/fastq_align_star/main.nf       | 1 -
 subworkflows/local/fusioncatcher_workflow/main.nf | 1 -
 subworkflows/local/fusioninspector_workflow.nf    | 1 -
 subworkflows/local/fusionreport_workflow/main.nf  | 1 -
 subworkflows/local/qc_workflow/main.nf            | 1 -
 subworkflows/local/starfusion_workflow/main.nf    | 1 -
 subworkflows/local/stringtie_workflow/main.nf     | 1 -
 subworkflows/local/trim_workflow/main.nf          | 1 -
 10 files changed, 11 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index aec6e6e03..e9952b3c2 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -280,7 +280,6 @@ The weights for databases are as follows:
 The Fusion Indication Index FII is calculated using two components:
 
 1. Tool Detection (50% of total FII)
-
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 
diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
index 64b106325..f74d26313 100644
--- a/modules/local/starfusion/detect/main.nf
+++ b/modules/local/starfusion/detect/main.nf
@@ -54,5 +54,3 @@ process STARFUSION {
     END_VERSIONS
     """
 }
-
-
diff --git a/subworkflows/local/fastq_align_star/main.nf b/subworkflows/local/fastq_align_star/main.nf
index 9f4620416..fe9848c39 100644
--- a/subworkflows/local/fastq_align_star/main.nf
+++ b/subworkflows/local/fastq_align_star/main.nf
@@ -53,4 +53,3 @@ workflow FASTQ_ALIGN_STAR {
     log_final     = STAR_ALIGN.out.log_final
     gene_count    = STAR_ALIGN.out.read_per_gene_tab
 }
-
diff --git a/subworkflows/local/fusioncatcher_workflow/main.nf b/subworkflows/local/fusioncatcher_workflow/main.nf
index bb878754f..d842fa291 100644
--- a/subworkflows/local/fusioncatcher_workflow/main.nf
+++ b/subworkflows/local/fusioncatcher_workflow/main.nf
@@ -45,4 +45,3 @@ workflow FUSIONCATCHER_WORKFLOW {
         fusions  = ch_fusioncatcher_fusions     // channel [ meta, fusions ]
         versions = ch_versions                  // channel [ versions      ]
     }
-
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index f33d2a86f..c54b3c7b4 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -68,4 +68,3 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         ch_arriba_visualisation
         versions             = ch_versions
 }
-
diff --git a/subworkflows/local/fusionreport_workflow/main.nf b/subworkflows/local/fusionreport_workflow/main.nf
index c4a896dd7..9343c95e8 100644
--- a/subworkflows/local/fusionreport_workflow/main.nf
+++ b/subworkflows/local/fusionreport_workflow/main.nf
@@ -32,4 +32,3 @@ workflow FUSIONREPORT_WORKFLOW {
         csv                      = ch_csv
 
 }
-
diff --git a/subworkflows/local/qc_workflow/main.nf b/subworkflows/local/qc_workflow/main.nf
index 9d9833f0a..a423471db 100644
--- a/subworkflows/local/qc_workflow/main.nf
+++ b/subworkflows/local/qc_workflow/main.nf
@@ -47,4 +47,3 @@ workflow QC_WORKFLOW {
         insertsize_metrics  = ch_insertsize_metrics  // channel [ meta, path ]
 
 }
-
diff --git a/subworkflows/local/starfusion_workflow/main.nf b/subworkflows/local/starfusion_workflow/main.nf
index 53f0dd8a4..642801163 100644
--- a/subworkflows/local/starfusion_workflow/main.nf
+++ b/subworkflows/local/starfusion_workflow/main.nf
@@ -25,4 +25,3 @@ workflow STARFUSION_WORKFLOW {
         fusions               = ch_starfusion_fusions
         versions              = ch_versions
     }
-
diff --git a/subworkflows/local/stringtie_workflow/main.nf b/subworkflows/local/stringtie_workflow/main.nf
index 28fa0547e..5d6d72fc7 100644
--- a/subworkflows/local/stringtie_workflow/main.nf
+++ b/subworkflows/local/stringtie_workflow/main.nf
@@ -34,4 +34,3 @@ workflow STRINGTIE_WORKFLOW {
         versions      = ch_versions                    // channel: [ path(versions.yml) ]
 
     }
-
diff --git a/subworkflows/local/trim_workflow/main.nf b/subworkflows/local/trim_workflow/main.nf
index d4d559f79..ec7b6199e 100644
--- a/subworkflows/local/trim_workflow/main.nf
+++ b/subworkflows/local/trim_workflow/main.nf
@@ -38,4 +38,3 @@ workflow TRIM_WORKFLOW {
         ch_fastqc_trimmed       // Channel [ meta, path_zip  ]
         versions = ch_versions  // Channel [ versions        ]
     }
-

From 4d2a71cf0967b615c4419335347428c36d781655 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 15:24:55 +0200
Subject: [PATCH 602/887] end-of-file

---
 docs/images/BTB_logo.svg        | 2 +-
 docs/images/NGI_logo.svg        | 2 +-
 docs/images/SciLifeLab_logo.svg | 2 +-
 docs/images/rnafusion_logo.svg  | 2 +-
 4 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/docs/images/BTB_logo.svg b/docs/images/BTB_logo.svg
index 099f1101f..fd3564060 100644
--- a/docs/images/BTB_logo.svg
+++ b/docs/images/BTB_logo.svg
@@ -181,4 +181,4 @@
          d="m 249.627,285.3818 c 0,-2.5 -1.717,-4.271 -4.126,-4.271 -2.412,0 -4.13,1.771 -4.13,4.271 0,2.496 1.718,4.277 4.13,4.277 2.409,0 4.126,-1.781 4.126,-4.277"
          style="fill:#33b540;fill-opacity:1;fill-rule:nonzero;stroke:none"
          id="path142"
-         inkscape:connector-curvature="0" /></g></g></svg>
\ No newline at end of file
+         inkscape:connector-curvature="0" /></g></g></svg>
diff --git a/docs/images/NGI_logo.svg b/docs/images/NGI_logo.svg
index aef40fd81..0a880c573 100644
--- a/docs/images/NGI_logo.svg
+++ b/docs/images/NGI_logo.svg
@@ -330,4 +330,4 @@
                  d="M 76.914,107 H 97.133 V 98.797 H 76.914 Z"
                  style="fill:#2e86ef;fill-opacity:1;fill-rule:evenodd;stroke:none"
                  id="path170"
-                 inkscape:connector-curvature="0" /></g></g></g></g></g></g></svg>
\ No newline at end of file
+                 inkscape:connector-curvature="0" /></g></g></g></g></g></g></svg>
diff --git a/docs/images/SciLifeLab_logo.svg b/docs/images/SciLifeLab_logo.svg
index b8a44b794..45d95b731 100644
--- a/docs/images/SciLifeLab_logo.svg
+++ b/docs/images/SciLifeLab_logo.svg
@@ -96,4 +96,4 @@
          d="m 265.398,144.591 c 3.801,0 7,3.101 7,7.101 0,3.801 -3.199,6.899 -7,6.899 -3.898,0 -7,-3.098 -7,-6.899 0,-4 3.102,-7.101 7,-7.101 z"
          style="fill:#7fcb28;fill-opacity:1;fill-rule:evenodd;stroke:none"
          id="path34"
-         inkscape:connector-curvature="0" /></g></g></svg>
\ No newline at end of file
+         inkscape:connector-curvature="0" /></g></g></svg>
diff --git a/docs/images/rnafusion_logo.svg b/docs/images/rnafusion_logo.svg
index fcd196c81..ff3631448 100644
--- a/docs/images/rnafusion_logo.svg
+++ b/docs/images/rnafusion_logo.svg
@@ -205,4 +205,4 @@
    d="m 300.43725,166.1155 -21.53224,21.61638 h 61.0915 V 166.1155 Z"
    id="path67"
    inkscape:connector-curvature="0"
-   style="fill:url(#f)" /></g></svg>
\ No newline at end of file
+   style="fill:url(#f)" /></g></svg>

From 90a6614e7f9e9affd16cfab00780d9337a11c906 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 15:26:13 +0200
Subject: [PATCH 603/887] update snap to ref only

---
 modules/nf-core/arriba/download/meta.yml |  69 ++--
 tests/test_build.nf.test                 |  20 +-
 tests/test_build.nf.test.snap            | 475 +----------------------
 3 files changed, 59 insertions(+), 505 deletions(-)

diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
index 06a8ef60d..abeb9f8fd 100644
--- a/modules/nf-core/arriba/download/meta.yml
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -16,44 +16,37 @@ tools:
       identifier: biotools:Arriba
 
 input:
-  - genome:
-      type: string
-      description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
-output:
-  blacklist:
-    - blacklist*${genome}*.tsv.gz:
+  - - genome:
         type: string
-        description: The blacklist removes recurrent alignment artifacts and transcripts
-          which are present in healthy tissue
-        pattern: ".tsv.gz"
-  cytobands:
-    - cytobands*${genome}*.tsv:
-        type: file
-        description: Coordinates of the Giemsa staining bands. This information is used
-          to draw ideograms
-        pattern: ".tsv"
-        ontologies:
-          - edam: http://edamontology.org/format_3475 # TSV
-  protein_domains:
-    - protein_domains*${genome}*.gff3:
-        type: file
-        description: Protein domain annotations
-        pattern: "*.gff3"
-        ontologies: []
-  known_fusions:
-    - known_fusions*${genome}*.tsv.gz:
-        type: file
-        description: Arriba is more sensitive to those fusions to improve the detection
-          rate of expected or highly relevant events, such as recurrent fusions
-        pattern: "*.tsv.gz"
-        ontologies:
-          - edam: http://edamontology.org/format_3989 # GZIP format
-  versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+        description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
+output:
+  - blacklist:
+      - blacklist*${genome}*.tsv.gz:
+          type: string
+          description: The blacklist removes recurrent alignment artifacts and transcripts
+            which are present in healthy tissue
+          pattern: ".tsv"
+  - cytobands:
+      - cytobands*${genome}*.tsv:
+          type: file
+          description: Coordinates of the Giemsa staining bands. This information is used
+            to draw ideograms
+          pattern: ".tsv"
+  - protein_domains:
+      - protein_domains*${genome}*.gff3:
+          type: file
+          description: Protein domain annotations
+          pattern: "*.gff3"
+  - known_fusions:
+      - known_fusions*${genome}*.tsv.gz:
+          type: file
+          description: Arriba is more sensitive to those fusions to improve the detection
+            rate of expected or highly relevant events, such as recurrent fusions
+          pattern: "*.tsv"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@peterpru"
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 04ca91a05..4157bffea 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -12,12 +12,20 @@ nextflow_pipeline {
 
         when {
             params {
-                outdir = "$outputDir"
-                outdir           = "$outputDir"
-                fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
-                input            = "${projectDir}/tests/yml/bam.yml"
-                tools            = "all"
-                no_cosmic        = true
+                config_profile_name        = 'Test build references profile'
+                config_profile_description = 'Minimal test dataset to check pipeline function'
+
+                // Input data
+                references_only            = true
+                input                      = "${projectDir}/tests/csv/fastq.csv"
+                no_cosmic                  = true
+                tools                      = "arriba,starfusion,fusionreport,salmon,fusioninspector"
+                fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'
+                gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
+                fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                species                    = 'homo_sapiens'
+                dfam_version               = '3.4'
+                outdir                     = "$outputDir"
             }
         }
 
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index f8d21222f..13f517314 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -1,48 +1,11 @@
 {
     "Test build references": {
         "content": [
-            35,
+            12,
             {
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.1"
-                },
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.5.0"
-                },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.5.0"
                 },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.5.0"
-                },
-                "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "CTATSPLICING_STARTOCANCERINTRONS": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "FASTP": {
-                    "fastp": "0.24.0"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FASTQC_FOR_FASTP": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
-                },
-                "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
-                },
-                "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
-                },
-                "FUSIONREPORT": {
-                    "fusion_report": "4.0.0"
-                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "4.0.0"
                 },
@@ -52,13 +15,6 @@
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
                 "GET_RRNA_TRANSCRIPTS": {
                     "get_rrna_transcripts": "v1.0"
                 },
@@ -71,24 +27,12 @@
                 "HGNC_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
                 "SALMON_INDEX": {
                     "salmon": "1.10.3"
                 },
-                "SALMON_QUANT": {
-                    "salmon": "1.10.3"
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
                 "STARFUSION_BUILD": {
                     "wget": "1.21.4",
                     "STAR-Fusion": "1.15.0"
@@ -98,201 +42,16 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.3"
-                },
-                "VCF_COLLECT": {
-                    "python": "3.11.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "agat",
-                "agat/test.tsv",
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
-                "ctatsplicing",
-                "ctatsplicing/test.cancer.introns",
-                "ctatsplicing/test.cancer.introns.prelim",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/test.chckpts",
-                "ctatsplicing/test.ctat-splicing.igv.html",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/test.igv.tracks",
-                "ctatsplicing/test.introns",
-                "ctatsplicing/test.introns.for_IGV.bed",
-                "fastp",
-                "fastp/test.fastp.html",
-                "fastp/test.fastp.json",
-                "fastp/test.fastp.log",
-                "fastp/test_1.fastp.fastq.gz",
-                "fastp/test_2.fastp.fastq.gz",
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
-                "fusioncatcher",
-                "fusioncatcher/test.fusion-genes.txt",
-                "fusioncatcher/test.log",
-                "fusioncatcher/test.summary.txt",
-                "fusioninspector",
-                "fusioninspector/test",
-                "fusioninspector/test/FusionInspector.log",
-                "fusioninspector/test/IGV_inputs",
-                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/test/IGV_inputs/cytoBand.txt",
-                "fusioninspector/test/IGV_inputs/test.ROI.bed",
-                "fusioninspector/test/IGV_inputs/test.bed",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.fa",
-                "fusioninspector/test/IGV_inputs/test.fa.fai",
-                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/test/IGV_inputs/test.gtf",
-                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/tracks.json",
-                "fusioninspector/test/chckpts_dir",
-                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/test/chckpts_dir/blast_filter.ok",
-                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/test/chckpts_dir/cp_final.ok",
-                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/test/chckpts_dir/cytoband.ok",
-                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/test/chckpts_dir/final.abridged.ok",
-                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/test/chckpts_dir/microH.dat.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/test/chckpts_dir/run_STAR.ok",
-                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/test/fi_workdir",
-                "fusioninspector/test/fi_workdir/Log.final.out",
-                "fusioninspector/test/fi_workdir/Log.out",
-                "fusioninspector/test/fi_workdir/Log.progress.out",
-                "fusioninspector/test/fi_workdir/SJ.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/test/fi_workdir/microH.dat",
-                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/test/fi_workdir/star_align.ok",
-                "fusioninspector/test/fi_workdir/test.fa",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/test/fi_workdir/test.gtf",
-                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test/test.fusion_inspector_web.html",
-                "fusionreport",
-                "fusionreport/test",
-                "fusionreport/test/AAA_BBB.html",
-                "fusionreport/test/test.fusionreport.tsv",
-                "fusionreport/test/test.fusionreport_filtered.tsv",
-                "fusionreport/test/test.fusions.csv",
-                "fusionreport/test/test.fusions.json",
-                "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "gatk4/minigenome.dict",
                 "get",
                 "get/rrna.bed",
                 "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
@@ -307,17 +66,12 @@
                 "references/fusion_report_db/fusion_report.log",
                 "references/fusion_report_db/fusiongdb2.db",
                 "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
                 "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/gencode/minigenome.fa.fai",
+                "references/gencode/minigenome.gtf.interval_list",
+                "references/gencode/minigenome.gtf.refflat",
                 "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
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                 "complete_ref_lens.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ctg_offsets.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "duplicate_clusters.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -676,11 +246,7 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cancer_splicing.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refGene.sort.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "refGene.sort.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -709,26 +275,13 @@
                 "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_lib_format_counts.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_meta_info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.Aligned.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "stringtie.merged.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.ballgown:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.coverage.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.gene.abundance.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.transcripts.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test_fusion_data.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T13:11:06.709587"
+        "timestamp": "2025-07-14T15:25:05.434296"
     }
 }
\ No newline at end of file

From ff88e3e72029fc617c69e7deab6853a398a47b45 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 15:57:22 +0200
Subject: [PATCH 604/887] update hgnc

---
 modules/local/hgnc/main.nf                 |  2 +-
 modules/local/hgnc/tests/main.nf.test      | 12 ++++++------
 modules/local/hgnc/tests/main.nf.test.snap | 14 ++++----------
 3 files changed, 11 insertions(+), 17 deletions(-)

diff --git a/modules/local/hgnc/main.nf b/modules/local/hgnc/main.nf
index 576e4800a..ee14e2c3b 100644
--- a/modules/local/hgnc/main.nf
+++ b/modules/local/hgnc/main.nf
@@ -10,7 +10,7 @@ process HGNC_DOWNLOAD {
     output:
     path "hgnc_complete_set.txt"        , emit: hgnc_ref
     path "HGNC-DB-timestamp.txt"        , emit: hgnc_date
-    path "versions.yml"   , emit: versions
+    path "versions.yml"                 , emit: versions
 
 
     script:
diff --git a/modules/local/hgnc/tests/main.nf.test b/modules/local/hgnc/tests/main.nf.test
index cecc4302d..795044d24 100644
--- a/modules/local/hgnc/tests/main.nf.test
+++ b/modules/local/hgnc/tests/main.nf.test
@@ -18,10 +18,10 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    process.out.hgnc_ref,
                     file(process.out.hgnc_date[0]).name,
-                    process.out.versions
-                ).match() }
+                ).match() },
+                { assert path(process.out.hgnc_ref[0]).exists() },
+                { assert path(process.out.versions[0]).exists() }
             )
         }
     }
@@ -42,10 +42,10 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    process.out.hgnc_ref,
                     file(process.out.hgnc_date[0]).name,
-                    process.out.versions
-                ).match() }
+                ).match() },
+                { assert path(process.out.hgnc_ref[0]).exists() },
+                { assert path(process.out.versions[0]).exists() }
             )
         }
     }
diff --git a/modules/local/hgnc/tests/main.nf.test.snap b/modules/local/hgnc/tests/main.nf.test.snap
index 8512dcf6e..1fd34b6a1 100644
--- a/modules/local/hgnc/tests/main.nf.test.snap
+++ b/modules/local/hgnc/tests/main.nf.test.snap
@@ -17,18 +17,12 @@
     },
     "Should download HGNC files": {
         "content": [
-            [
-                "hgnc_complete_set.txt:md5,29571d88d1648e8764b70791df6a5d2d"
-            ],
-            "HGNC-DB-timestamp.txt",
-            [
-                "versions.yml:md5,a7b7ccbd9eda8036baf548cdf1cb6867"
-            ]
+            "HGNC-DB-timestamp.txt"
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.1"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-11-26T17:41:49.965512273"
+        "timestamp": "2025-07-14T15:48:18.667694"
     }
 }
\ No newline at end of file

From 33eabe213b58ecc375babbac808ae228222cfd07 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 16:06:05 +0200
Subject: [PATCH 605/887] update snap test_stub

---
 tests/test_stub.nf.test      | 4 ++++
 tests/test_stub.nf.test.snap | 8 ++++----
 2 files changed, 8 insertions(+), 4 deletions(-)

diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index f9039416c..495d20b3d 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -14,6 +14,8 @@ nextflow_pipeline {
             params {
                 outdir           = "$outputDir"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                tools            = "all"
+                no_cosmic              = true
             }
         }
 
@@ -239,6 +241,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "fusionreport,arriba,starfusion,fusioncatcher"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                no_cosmic        = true
             }
         }
 
@@ -335,6 +338,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "fusioninspector,arriba,fusionreport"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                no_cosmic        = true
             }
         }
 
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 6d1f04f20..d31bab4e5 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -870,14 +870,14 @@
                 "star/test.unmapped_2.fastq.gz",
                 "star/testXd.out.bam",
                 "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "vcf/test_fusion_data.vcf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-11T15:18:18.890499282"
+        "timestamp": "2025-07-14T16:00:28.831228"
     },
     "stub test salmon": {
         "content": [
@@ -2171,13 +2171,13 @@
                 "stringtie/test.gene.abundance.txt",
                 "stringtie/test.transcripts.gtf",
                 "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "vcf/test_fusion_data.vcf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-11T15:05:16.899145218"
+        "timestamp": "2025-07-14T15:39:15.996857"
     }
 }
\ No newline at end of file

From e86c41bb33b2c26eddb29a401c8b1a164aa1f70d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 16:13:08 +0200
Subject: [PATCH 606/887] update fusionreport

---
 .../fusionreport_workflow/tests/main.nf.test      |  6 +++---
 .../fusionreport_workflow/tests/main.nf.test.snap | 15 ++-------------
 2 files changed, 5 insertions(+), 16 deletions(-)

diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
index 63e44f1f8..cbff19656 100644
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
@@ -59,10 +59,10 @@ nextflow_workflow {
                 { assert snapshot(
                     workflow.out.fusion_list,
                     workflow.out.fusion_list_filtered,
-                    workflow.out.report,
                     workflow.out.csv,
-                    workflow.out.versions
-                ).match() }
+                ).match() },
+                { assert path(workflow.out.report[0][1]).exists() },  // Extract the file path
+                { assert path(workflow.out.versions[0]).exists() },
             )
         }
     }
diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap b/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
index 4dd686d8a..df7b469d1 100644
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
@@ -17,14 +17,6 @@
                     "test_sample.fusionreport_filtered.tsv:md5,3593b7021f26cc5427fdc96f0d1c72f0"
                 ]
             ],
-            [
-                [
-                    {
-                        "id": "test_sample"
-                    },
-                    "test_sample_fusionreport_index.html:md5,9dfbf94471173bebbd8085e440515e4d"
-                ]
-            ],
             [
                 [
                     {
@@ -32,15 +24,12 @@
                     },
                     "test_sample.fusions.csv:md5,ee6c6838110d68bc75a13c19dca0a321"
                 ]
-            ],
-            [
-                "versions.yml:md5,5b80da331704f6d2093b7609537222e8"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T11:32:39.322725"
+        "timestamp": "2025-07-14T15:59:24.708716"
     }
 }
\ No newline at end of file

From 636fe53665d3c6e94fba013e1c2f072d2b93f259 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 14 Jul 2025 15:00:36 +0000
Subject: [PATCH 607/887] Format: Import nf-core modules

---
 subworkflows/local/build_references/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index d3bcf108c..43feb4935 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -6,9 +6,7 @@
 
 include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode_download/main'
 include { FUSIONCATCHER_DOWNLOAD }          from '../../../modules/local/fusioncatcher/download/main'
-include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/nf-core/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
-include { STARFUSION_BUILD }                from '../../../modules/nf-core/starfusion/build/main'
 include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
 include { GET_RRNA_TRANSCRIPTS }            from '../../../modules/local/get_rrna_transcript/main'
 include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatsplicing/prepgenomelib/main.nf'
@@ -25,6 +23,8 @@ include { STAR_GENOMEGENERATE }             from '../../../modules/nf-core/star/
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../../modules/nf-core/gatk4/createsequencedictionary/main'
 include { GATK4_BEDTOINTERVALLIST }         from '../../../modules/nf-core/gatk4/bedtointervallist/main'
 include { SALMON_INDEX }                    from '../../../modules/nf-core/salmon/index/main'
+include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/nf-core/fusionreport/download/main'
+include { STARFUSION_BUILD }                from '../../../modules/nf-core/starfusion/build/main'
 include { GFFREAD }                         from '../../../modules/nf-core/gffread/main'
 include { getFileSuffix } from '../../../modules/nf-core/cat/cat/main.nf'
 

From b76c8bbfdd6ae25bc4319a56da0187771a537961 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 17:08:43 +0200
Subject: [PATCH 608/887] update snaps

---
 .../prepgenomelib/tests/main.nf.test          |  5 +-
 .../starfusion/detect/tests/main.nf.test      |  4 +-
 .../local/qc_workflow/test/main.nf.test       | 69 +++++++--------
 .../starfusion_workflow/tests/main.nf.test    |  4 +-
 .../local/trim_workflow/tests/main.nf.test    | 47 ++++------
 .../trim_workflow/tests/main.nf.test.snap     | 85 +++++++++++++++++--
 6 files changed, 131 insertions(+), 83 deletions(-)

diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
index de69cd3e2..fd665f6ac 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -19,8 +19,9 @@ nextflow_process {
                 ]
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
-                """
+                input [3] = "homo_sapiens"
+                input [4] = "3.8"
+                input [5] = "37.4"                """
             }
         }
     }
diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
index 75e0bfdd7..a01a60f9f 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test
+++ b/modules/local/starfusion/detect/tests/main.nf.test
@@ -19,7 +19,9 @@ nextflow_process {
                 ]
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
+                sinput [3] = "homo_sapiens"
+                input [4] = "3.8"
+                input [5] = "37.4"
                 """
             }
         }
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index cfb48e06d..ae36becb6 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -13,50 +13,46 @@ nextflow_workflow {
         setup {
 
             // Create refflat reference
-            run("UCSC_GTFTOGENEPRED") {
-                script "../../../../modules/nf-core/ucsc/gtftogenepred/main.nf"
+            run("GTF_TO_REFFLAT") {
+                script "../../../../modules/local/uscs/custom_gtftogenepred/main.nf"
                 process {
                     """
-                    input[0] =
-                        Channel.fromPath(
-                            "https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf",
-                            checkIfExists: true
-                        )
-                        .map{ [ [id:it.Name], it ] }
+                    input[0] =Channel.of(
+                        [
+                            [id: 'gtf'],
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
+                        ]
+                    )
                     """
                 }
             }
 
             // Filter GTF to extract rRNA genes
-            run("RRNATRANSCRIPTS") {
-                script "../../../../modules/nf-core/rrnatranscripts/main.nf"
+            run("GET_RRNA_TRANSCRIPTS") {
+                script "../../../../modules/local/get_rrna_transcript/main.nf"
                 process {
                     """
-                    input[0] = Channel.fromPath("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
+                    input[0] = Channel.of(
+                        [
+                            [id: 'fasta'],
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExist: true)
+                        ]
+                    )
                     """
                 }
             }
 
-            // Convert rRNA GTF to BED
-            run("BEDOPS_CONVERT2BED") {
-                script "../../../../modules/nf-core/bedops/convert2bed/main.nf"
-                process {
-                    """
-                    input[0] = RRNATRANSCRIPTS.out.rrna_gtf.map{ it -> [ [id:it.Name], it ] }
-                    """
-                }
-            }
 
             // Convert rRNA BED to interval list (the necessary file)
             run("GATK4_BEDTOINTERVALLIST") {
                 script "../../../../modules/nf-core/gatk4/bedtointervallist/main.nf"
                 process {
                     """
-                    input[0] = BEDOPS_CONVERT2BED.out.bed
+                    input[0] = GET_RRNA_TRANSCRIPTS.out.bed
                     input[1] = Channel.of(
                         [
                             [id: 'chr22_dic'],
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.dic", checkIfExist: true)
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.dict", checkIfExist: true)
                         ]
                     )
                     """
@@ -83,32 +79,25 @@ nextflow_workflow {
                         file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
                     ])
 
-                // ch_chrgtf
-                input[1] = Channel.of(
-                    [
-                        [ id: "chr22_gtf" ],
-                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
-                    ])
-
                 // ch_refflat
-                input[2] = UCSC_GTFTOGENEPRED.out.refflat
-
+                input[1] = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
                 // ch_fasta
-                input[3] = Channel.of(
+                input[2] = Channel.of(
                     [
                         [ id: "test_ref" ],
                         file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta", checkIfExist: true)
-                    ] )
+                    ]
+                )
 
                 // ch_fai
-                input[4] = Channel.of(
+                input[3] = Channel.of(
                     [
                         [ id: "test_ref" ],
                         file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai", checkIfExist: true)
                     ] )
 
                 // ch rRNA interval list
-                input[5] = GATK4_BEDTOINTERVALLIST.out.interval_list
+                input[4] = GATK4_BEDTOINTERVALLIST.out.interval_list
                 """
             }
         }
@@ -116,10 +105,12 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { assert snapshot(file( workflow.out.versions[0] )).match('versions' )                                                },
-                { assert snapshot(file( workflow.out.rnaseq_metrics[0][1]     ).readLines()[4..-1]).md5().match('rnaseq_metrics'    ) },
-                { assert snapshot(file( workflow.out.duplicate_metrics[0][1]  ).readLines()[4..-1]).md5().match('duplicate_metrics' ) },
-                { assert snapshot(file( workflow.out.insertsize_metrics[0][1] ).readLines()[4..-1]).md5().match('insertsize_metrics') }
+                { assert snapshot(
+					file(workflow.out.rnaseq_metrics[0][1]).readLines()[5..8],
+					file(workflow.out.duplicate_metrics[0][1]).readLines()[5..8],
+					file(workflow.out.insertsize_metrics[0][1]).readLines()[5..8],
+                    workflow.out.versions
+                ).match() }
             )
         }
     }
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
index af6b117ff..a74c7e3ad 100644
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test
+++ b/subworkflows/local/starfusion_workflow/tests/main.nf.test
@@ -46,7 +46,9 @@ nextflow_workflow {
                 ]
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
+                input [3] = "homo_sapiens"
+                input [4] = "3.8"
+                input [5] = "37.4"
                 """
             }
         }
diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test b/subworkflows/local/trim_workflow/tests/main.nf.test
index 2f7568d6d..3151ebb2b 100644
--- a/subworkflows/local/trim_workflow/tests/main.nf.test
+++ b/subworkflows/local/trim_workflow/tests/main.nf.test
@@ -8,7 +8,7 @@ nextflow_workflow {
     tag "fastqc"
     tag "fastp"
 
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true") {
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true") {
 
         when {
 
@@ -32,21 +32,16 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { with(workflow.out) {
-                    assert snapshot(
-                        versions.collect{ file(it) },
-                        ch_reads_all[0][1].collect { file(it) },
-                        ch_reads_fusioncatcher[0][1].collect { file(it) },
-                        file(ch_fastp_html[0][1]).name,
-                        file(ch_fastp_json[0][1]),
-                        ch_fastqc_trimmed[0][1].collect { file(it).name }
-                    ).match() }
-                }
+                { assert snapshot(
+                    workflow.out.ch_reads_all,
+                    workflow.out.ch_fastp_json,
+                    workflow.out.versions
+                ).match() },
             )
         }
     }
 
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true + fasp_adaptors") {
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true + fastp_adaptors") {
 
         when {
 
@@ -70,21 +65,16 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { with(workflow.out) {
-                    assert snapshot(
-                        versions.collect{ file(it) },
-                        ch_reads_all[0][1].collect { file(it) },
-                        ch_reads_fusioncatcher[0][1].collect { file(it) },
-                        file(ch_fastp_html[0][1]).name,
-                        file(ch_fastp_json[0][1]),
-                        ch_fastqc_trimmed[0][1].collect { file(it).name }
+                { assert snapshot(
+                        workflow.out.ch_reads_all,
+                        workflow.out.ch_fastp_json,
+                        workflow.out.versions
                     ).match() }
-                }
             )
         }
     }
 
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == false") {
+    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp skip_qc== false") {
 
         when {
 
@@ -108,16 +98,11 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { with(workflow.out) {
-                    assert snapshot(
-                        versions.size() == 0,
-                        ch_reads_all[0][1].size() == 2,
-                        ch_reads_fusioncatcher[0][1].size() == 2,
-                        ch_fastp_html.size() == 0,
-                        ch_fastp_json.size() == 0,
-                        ch_fastqc_trimmed.size() == 0
+                { assert snapshot(
+                        workflow.out.ch_reads_all,
+                        workflow.out.ch_fastp_json,
+                        workflow.out.versions
                     ).match() }
-                }
             )
         }
     }
diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test.snap b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
index ae9f9e2e0..829c57265 100644
--- a/subworkflows/local/trim_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
@@ -1,4 +1,35 @@
 {
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true + fastp_adaptors": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test_fastq"
+                    },
+                    [
+                        "test_fastq_1.fastp.fastq.gz:md5,adc67a7b4d0bf3520866d7599a4ba814",
+                        "test_fastq_2.fastp.fastq.gz:md5,9ee7d6c5230442970997477464255e67"
+                    ]
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test_fastq"
+                    },
+                    "test_fastq.fastp.json:md5,38711d953a615ef8d88edceaa518d2ac"
+                ]
+            ],
+            [
+                "versions.yml:md5,bc4adfa947f4bf5a3558bcf09213841c"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-14T17:07:52.648611"
+    },
     "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true": {
         "content": [
             [
@@ -26,20 +57,56 @@
         },
         "timestamp": "2024-12-05T16:21:52.926289296"
     },
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp skip_qc== false": {
+        "content": [
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-14T16:43:42.01598"
+    },
+    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true": {
+        "content": [
+            [
+                
+            ],
+            [
+                
+            ],
+            [
+                
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-14T17:02:48.598962"
+    },
     "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == false": {
         "content": [
-            true,
-            true,
-            true,
-            true,
-            true,
-            true
+            [
+                
+            ],
+            [
+                
+            ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-12-05T16:22:45.877168833"
+        "timestamp": "2025-07-14T16:22:58.668715"
     },
     "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true + fasp_adaptors": {
         "content": [

From 07ca9cf0eb3efdc8360e30efe08cc310675d2119 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 17:20:58 +0200
Subject: [PATCH 609/887] update tests

---
 .../starfusion_workflow/tests/main.nf.test    | 33 +++----------------
 1 file changed, 4 insertions(+), 29 deletions(-)

diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
index a74c7e3ad..ce77ba6d7 100644
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test
+++ b/subworkflows/local/starfusion_workflow/tests/main.nf.test
@@ -7,6 +7,7 @@ nextflow_workflow {
     tag "star"
     tag "star/genomegenerate"
     tag "star/align"
+    options "-stub"
 
 
     test("STARFUSION_WORKFLOW - Homo sapiens - FASTQs minigenome") {
@@ -57,36 +58,10 @@ nextflow_workflow {
         when {
             workflow {
                 """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [ id: "test_fastqs" ],
-                        [
-                            file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz", checkIfExists: true),
-                            file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz", checkIfExists: true)
-                        ]
-                    ] )
-
-                // ch_gtf
-                input[1] =
-                    Channel.fromPath(
-                        "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
-                // ch_starindex_ref
-                input[2] = STAR_GENOMEGENERATE.out.index
-
-                // ch_fasta
-                input[3] =
-                    Channel.fromPath(
-                        "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa", checkIfExists: true
-                    )
-                    .map{ [ [ id: it.name ], it ] }
-
+                input[0] = []
                 // ch_starfusion_ref
-                input[4] = STARFUSION_BUILD.out.reference
-
+                input[1] = STARFUSION_BUILD.out.reference
+                input[2] = []
                 """
             }
             params {

From 02e77e02ffcb5cf5e691360b15de25ea571e5874 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 14 Jul 2025 17:27:02 +0200
Subject: [PATCH 610/887] update tests

---
 tests/test_stub_bam.nf.test      | 1 +
 tests/test_stub_bam.nf.test.snap | 8 ++++----
 2 files changed, 5 insertions(+), 4 deletions(-)

diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
index 8f080c808..cea94105e 100644
--- a/tests/test_stub_bam.nf.test
+++ b/tests/test_stub_bam.nf.test
@@ -318,6 +318,7 @@ nextflow_pipeline {
                 tools            = "fusioninspector,arriba,fusionreport"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                no_cosmic        = true
             }
         }
 
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index c5bbb1354..ffb4aaf1c 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -768,14 +768,14 @@
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "vcf/test_fusion_data.vcf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-11T14:56:00.138311656"
+        "timestamp": "2025-07-14T16:53:36.788101"
     },
     "stub test salmon": {
         "content": [
@@ -1969,13 +1969,13 @@
                 "stringtie/test.gene.abundance.txt",
                 "stringtie/test.transcripts.gtf",
                 "vcf",
-                "vcf/test_fusion_data.vcf.gz"
+                "vcf/test_fusion_data.vcf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-11T14:43:32.816080551"
+        "timestamp": "2025-07-14T16:09:39.371336"
     }
 }
\ No newline at end of file

From a73b727ac064a46580132a55a7726d4f6a1b0ff9 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 14 Jul 2025 17:00:38 +0000
Subject: [PATCH 611/887] test: Update tests

---
 .../starfusion_workflow/tests/main.nf.test    |  21 +-
 tests/test_build.nf.test.snap                 | 175 ++--
 tests/test_stub.nf.test.snap                  | 809 +++++++++---------
 tests/test_stub_bam.nf.test.snap              | 805 ++++++++---------
 4 files changed, 924 insertions(+), 886 deletions(-)

diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
index 34ec9f35a..c7f49ac5b 100644
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test
+++ b/subworkflows/local/starfusion_workflow/tests/main.nf.test
@@ -37,16 +37,25 @@ nextflow_workflow {
             process {
                 """
                 input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                    [ id:'minigenome_fasta' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
                 ]
                 input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                    [ id:'minigenome_gtf' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
                 ]
 
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "human"
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                input[3] = "homo_sapiens"
+                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[5] = [
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                ]
+                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                 """
             }
         }
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index d92113b8a..2f5f47d86 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -34,8 +34,9 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -109,81 +110,81 @@
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
                 "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz"
+                "references/starfusion/minigenome.fa_genome_lib_build_dir",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/minigenome.fa_genome_lib_build_dir/trans.blast.dat.gz"
             ],
             [
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
@@ -221,6 +222,7 @@
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
+                "PFAM.domtblout.dat.gz:md5,76acbc468fafae81dfa43592eef493fc",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -244,20 +246,35 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.dat.gz:md5,a5b06bb2693d2d77e1938394f7793c7d",
+                "blast_pairs.idx:md5,0828b964ba35570ad8e16f98fac59c27",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                "fusion_annot_lib.idx:md5,aefd7a66969fcb070bb3c2a582b4a5ec",
+                "pfam_domains.dbm:md5,db9fe9867cb32adea6a49daa8daed9f0",
+                "ref_annot.cdna.fa:md5,44cdca7529e62445f24585a744042bf6",
+                "ref_annot.cdna.fa.idx:md5,ad9b2ff39db537a5bfabcf0de5e5436b",
+                "ref_annot.cds:md5,c8bd5fd597999dd9fbe5f3e1ce608b92",
+                "ref_annot.cdsplus.fa:md5,71beac58d4b5c868a53eac4281231e0e",
+                "ref_annot.cdsplus.fa.idx:md5,76a779cf017fd9a46a0b14e87676cf55",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                "ref_annot.gtf.gene_spans:md5,f40268a9e150d8b7a1a48b0e72ac60c9",
                 "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                 "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                "ref_annot.pep:md5,ec98534b89cc7c4e401e3b4314988015",
+                "ref_annot.prot_info.dbm:md5,b9a80f43e28568ad86b5f5477df1d5c1",
                 "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                 "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                 "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                 "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                "ref_genome.fa.nin:md5,3f655819c4ad2fa557c47c8a95332be0",
+                "ref_genome.fa.njs:md5,bdd75db6a8b2a70775e60c921edf9cd4",
                 "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                 "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                 "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                 "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                "Log.out:md5,413480634740d0030704ce1eab1ea877",
                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -268,11 +285,13 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "genomeParameters.txt:md5,828a5e6257ca2a79bcdb9a1f3ded7d8d",
                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                "trans.blast.align_coords.align_coords.dbm:md5,bfcff23c4fa5cdd0e5d3f536cce5b815",
                 "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
             ]
         ],
@@ -280,6 +299,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T14:00:33.582495752"
+        "timestamp": "2025-07-14T15:29:12.576749759"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index a2ecc9705..3257e66a5 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -41,8 +41,9 @@
                     "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -107,81 +108,81 @@
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
                 "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/fusion_annot_lib.idx",
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -209,9 +210,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-16T09:22:13.085521"
+        "timestamp": "2025-07-14T16:05:43.050813403"
     },
     "stub test stringtie": {
         "content": [
@@ -557,8 +558,9 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -775,81 +777,81 @@
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
                 "references/starfusion",
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-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
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-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/AnnotFilterRule.pm",
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -877,7 +879,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T15:06:14.950970078"
+        "timestamp": "2025-07-14T16:59:31.489136352"
     },
     "stub test salmon": {
         "content": [
@@ -1015,8 +1017,9 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1093,86 +1096,86 @@
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -1196,9 +1199,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-13T19:05:06.954764"
+        "timestamp": "2025-07-14T16:03:10.060575954"
     },
     "stub test fusionreport": {
         "content": [
@@ -1261,8 +1264,9 @@
                     "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -1356,81 +1360,81 @@
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -1460,7 +1464,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T15:04:39.201458666"
+        "timestamp": "2025-07-14T16:07:58.783505822"
     },
     "stub test fastp": {
         "content": [
@@ -1785,8 +1789,9 @@
                     "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
@@ -2056,86 +2061,86 @@
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -2178,6 +2183,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T15:01:11.405363456"
+        "timestamp": "2025-07-14T16:00:32.296879783"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 16f2f89c3..defba9a4e 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -38,8 +38,9 @@
                     "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -99,81 +100,81 @@
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
                 "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "starfusion",
                 "starfusion/test.starfusion.abridged.coding_effect.tsv",
                 "starfusion/test.starfusion.abridged.tsv",
@@ -184,7 +185,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T10:14:43.131367"
+        "timestamp": "2025-07-14T15:48:07.635249587"
     },
     "stub test stringtie": {
         "content": [
@@ -476,8 +477,9 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -689,81 +691,81 @@
                 "references/star/sjdbList.out.tab",
                 "references/star/transcriptInfo.tab",
                 "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -775,7 +777,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T14:58:49.830786101"
+        "timestamp": "2025-07-14T15:52:47.318544583"
     },
     "stub test salmon": {
         "content": [
@@ -929,8 +931,9 @@
                     "samtools": 1.21
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -1002,86 +1005,86 @@
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai"
@@ -1091,7 +1094,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T09:40:30.61019"
+        "timestamp": "2025-07-14T15:46:23.95688627"
     },
     "stub test fusionreport": {
         "content": [
@@ -1151,8 +1154,9 @@
                     "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -1241,81 +1245,81 @@
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                 "references/star/transcriptInfo.tab",
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "starfusion",
                 "starfusion/test.starfusion.abridged.coding_effect.tsv",
                 "starfusion/test.starfusion.abridged.tsv",
@@ -1326,7 +1330,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T14:57:00.562428474"
+        "timestamp": "2025-07-14T15:50:04.584455613"
     },
     "stub test fastp": {
         "content": [
@@ -1604,8 +1608,9 @@
                     "STAR-Fusion": "1.15.0"
                 },
                 "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
                 },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
@@ -1870,86 +1875,86 @@
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+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/cancer_splicing_lib",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/refGene.bed",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -1976,6 +1981,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T14:53:35.614724758"
+        "timestamp": "2025-07-14T15:44:19.150388777"
     }
 }
\ No newline at end of file

From 2389bda1310c5d1a94b8a7c273895b44320bc666 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 14 Jul 2025 19:18:40 +0000
Subject: [PATCH 612/887] Add params to avoid hardcoding URLs

---
 nextflow.config                             |  7 +++++++
 subworkflows/local/build_references/main.nf | 14 ++++++--------
 2 files changed, 13 insertions(+), 8 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 9f5536013..c75e9b31f 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -85,6 +85,13 @@ params {
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
     ctatsplicing_cancer_introns   = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
+    dfam_hmm                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"
+    dfam_h3f                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f"
+    dfam_h3i                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i"
+    dfam_h3m                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3,"
+    dfam_h3p                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p"
+    pfam_url                      = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"
+    annot_filter_url              = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
 
     // Path to fusion outputs
     arriba_fusions                = null
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 43feb4935..083a0e093 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -171,16 +171,14 @@ workflow BUILD_REFERENCES {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
             }
-            pfam_url     = Channel.of(["http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"])
             dfam_urls_ch = Channel.of([
-                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm",
-                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f",
-                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i",
-                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m",
-                "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p"
+                params.dfam_hmm,
+                params.dfam_h3f,
+                params.dfam_h3i,
+                params.dfam_h3m,
+                params.dfam_h3p
             ])
-            annot_filter = Channel.of(["https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"])
-            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, pfam_url, dfam_urls_ch, annot_filter)
+            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, params.pfam_url, dfam_urls_ch, params.annot_filter_url)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
                 CTATSPLICING_PREPGENOMELIB(

From 0498949bcca1dfd2ed88d0c29221ed0540252305 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 15 Jul 2025 09:08:30 +0200
Subject: [PATCH 613/887] remove starfusion workflow (will be moved to modules)

---
 .../starfusion_workflow/tests/main.nf.test    | 88 -------------------
 .../tests/main.nf.test.snap                   | 38 --------
 .../starfusion_workflow/tests/nextflow.config | 33 -------
 3 files changed, 159 deletions(-)
 delete mode 100644 subworkflows/local/starfusion_workflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
 delete mode 100644 subworkflows/local/starfusion_workflow/tests/nextflow.config

diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test b/subworkflows/local/starfusion_workflow/tests/main.nf.test
deleted file mode 100644
index ce77ba6d7..000000000
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test
+++ /dev/null
@@ -1,88 +0,0 @@
-nextflow_workflow {
-
-    name "Test Subworkflow STARFUSION_WORKFLOW"
-    script "../main.nf"
-    workflow "STARFUSION_WORKFLOW"
-    tag "subworkflow"
-    tag "star"
-    tag "star/genomegenerate"
-    tag "star/align"
-    options "-stub"
-
-
-    test("STARFUSION_WORKFLOW - Homo sapiens - FASTQs minigenome") {
-        config './nextflow.config'
-
-        setup {
-            run("STAR_GENOMEGENERATE") {
-                script "../../../../modules/nf-core/star/genomegenerate/main.nf"
-                process {
-                """
-                    // FASTA
-                    input[0] = Channel.fromPath(
-                            "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-
-                    // GTF
-                    input[1] = Channel.fromPath(
-                            "https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf", checkIfExists: true
-                        )
-                        .map{ [[id: it.getName() ], it ]}
-                    """
-                }
-            }
-
-            run("STARFUSION_BUILD") {
-            script "../../../../modules/local/starfusion/build/main.nf"
-            process {
-                """
-                input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
-                ]
-                input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
-                ]
-
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "homo_sapiens"
-                input [4] = "3.8"
-                input [5] = "37.4"
-                """
-            }
-        }
-        }
-
-        when {
-            workflow {
-                """
-                input[0] = []
-                // ch_starfusion_ref
-                input[1] = STARFUSION_BUILD.out.reference
-                input[2] = []
-                """
-            }
-            params {
-                starfusion = true
-                cram = 'starfusion'
-                outdir = "$outputDir"
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(
-                    workflow.out.fusions,
-                    workflow.out.versions
-                ).match() }
-            )
-        }
-    }
-
-
-
-
-}
diff --git a/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap b/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 5bc897e7f..000000000
--- a/subworkflows/local/starfusion_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,38 +0,0 @@
-{
-    "STARFUSION_WORKFLOW - Homo sapiens - FASTQs minigenome": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test_fastqs"
-                    },
-                    "test_fastqs.starfusion.fusion_predictions.tsv:md5,abe17134a231642edf9351e4964e8a97"
-                ]
-            ],
-            "test_fastqs.Log.final.out",
-            [
-                [
-                    {
-                        "id": "test_fastqs"
-                    },
-                    "test_fastqs.ReadsPerGene.out.tab:md5,8e0d42deeea09924d5c7ba3147bbfd78"
-                ]
-            ],
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                "versions.yml:md5,afde4f2fd6056df81e322b3c35ab7a8a",
-                "versions.yml:md5,e53f1ec32bc78a33f99892e42274833a"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
-        },
-        "timestamp": "2024-12-23T18:25:52.838411738"
-    }
-}
\ No newline at end of file
diff --git a/subworkflows/local/starfusion_workflow/tests/nextflow.config b/subworkflows/local/starfusion_workflow/tests/nextflow.config
deleted file mode 100644
index 2f955abc4..000000000
--- a/subworkflows/local/starfusion_workflow/tests/nextflow.config
+++ /dev/null
@@ -1,33 +0,0 @@
-process {
-    withName: 'STAR_FOR_STARFUSION' {
-        ext.args = '--twopassMode Basic \
-        --outReadsUnmapped None \
-        --readFilesCommand zcat \
-        --outSAMtype BAM SortedByCoordinate \
-        --outSAMstrandField intronMotif \
-        --outSAMunmapped Within \
-        --chimSegmentMin 12 \
-        --chimJunctionOverhangMin 8 \
-        --chimOutJunctionFormat 1 \
-        --alignSJDBoverhangMin 10 \
-        --alignMatesGapMax 100000 \
-        --alignIntronMax 100000 \
-        --alignSJstitchMismatchNmax 5 -1 5 5 \
-        --chimMultimapScoreRange 3 \
-        --chimScoreJunctionNonGTAG -4 \
-        --chimMultimapNmax 20 \
-        --chimNonchimScoreDropMin 10 \
-        --peOverlapNbasesMin 12 \
-        --peOverlapMMp 0.1 \
-        --alignInsertionFlush Right \
-        --alignSplicedMateMapLminOverLmate 0 \
-        --alignSplicedMateMapLmin 30 \
-        --chimOutType Junctions \
-        --quantMode GeneCounts'
-    }
-
-        withName: 'SAMTOOLS_INDEX_FOR_STARFUSION_CRAM' {
-        ext.prefix = { "${meta.id}.star_for_starfusion.Aligned.sortedByCoord.out" }
-    }
-
-}

From 0ba3c8602651baa7c379687ddab177d6d1403775 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 15 Jul 2025 09:11:35 +0200
Subject: [PATCH 614/887] update changelog

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3671081f3..36b9a7534 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -83,6 +83,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated all `wget` containers to `conda-forge::wget=1.21.4` [#655](https://github.com/nf-core/rnafusion/pull/655)
 - Using FusionInspector abridged output (that now contains coding effects) for downstream analysis [#669](https://github.com/nf-core/rnafusion/pull/669)
 - Replaced local `FUSIONREPORT` and `FUSIONREPORT_DOWNLOAD` with `FUSIONREPORT_DETECT` and `FUSIONREPORT_DOWNLOAD` from nf-core [#703](https://github.com/nf-core/rnafusion/pull/703)
+- Updated arriba to v2.5.0 [#693](https://github.com/nf-core/rnafusion/pull/693)
 
 ### Fixed
 
@@ -101,6 +102,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix bug in argument handling for FusionInspector [#669](https://github.com/nf-core/rnafusion/pull/669)
 - Upgrade STAR-Fusion to 1.15.1 to solve problem building STAR-Fusion references with `--human_gencode_filter` [#683](https://github.com/nf-core/rnafusion/pull/683)
 - Fix missing memory unit for fusioncatcher [#674](https://github.com/nf-core/rnafusion/pull/674)
+- Fix fusioncatcher download link [#693](https://github.com/nf-core/rnafusion/pull/693)
 
 ### Removed
 

From 945b3a55813e9f58a869154fbff3a9722f7f3da9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 15 Jul 2025 13:32:15 +0200
Subject: [PATCH 615/887] re-upload arriba

---
 modules.json                                  |  2 +-
 .../nf-core/arriba/download/environment.yml   |  3 +-
 modules/nf-core/arriba/download/main.nf       | 34 ++++-----
 modules/nf-core/arriba/download/meta.yml      | 69 ++++++++++---------
 .../arriba/download/tests/main.nf.test.snap   | 24 +++----
 5 files changed, 71 insertions(+), 61 deletions(-)

diff --git a/modules.json b/modules.json
index df7353ccb..f42340fbf 100644
--- a/modules.json
+++ b/modules.json
@@ -17,7 +17,7 @@
                     },
                     "arriba/download": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "98bd7be6d2191b0d5900ee698d91b52eeab90311",
                         "installed_by": ["modules"]
                     },
                     "cat/cat": {
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
index e8f5b278f..b30731312 100644
--- a/modules/nf-core/arriba/download/environment.yml
+++ b/modules/nf-core/arriba/download/environment.yml
@@ -4,4 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::arriba=2.4.0
+  - bioconda::arriba=2.5.0
+  - conda-forge::wget=1.21.4
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
index c45120adb..baaf72f24 100644
--- a/modules/nf-core/arriba/download/main.nf
+++ b/modules/nf-core/arriba/download/main.nf
@@ -4,29 +4,30 @@ process ARRIBA_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/arriba:2.4.0--h0033a41_2' :
-        'biocontainers/arriba:2.4.0--h0033a41_2' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/27/27475cdcdbcc8c0ffb6b5ca8c2e6567dbe490edb96f5df4e8f01f4f95912dcd3/data' :
+        'community.wave.seqera.io/library/arriba_wget:a3e48cf793a0b654' }"
 
     input:
     val(genome)
 
     output:
-    path "blacklist*${genome}*.tsv.gz"       , emit: blacklist
-    path "cytobands*${genome}*.tsv"          , emit: cytobands
-    path "protein_domains*${genome}*.gff3"   , emit: protein_domains
-    path "known_fusions*${genome}*.tsv.gz"   , emit: known_fusions
-    path "versions.yml"                      , emit: versions
+    path "blacklist*${genome}*.tsv.gz"    , emit: blacklist
+    path "cytobands*${genome}*.tsv"       , emit: cytobands
+    path "protein_domains*${genome}*.gff3", emit: protein_domains
+    path "known_fusions*${genome}*.tsv.gz", emit: known_fusions
+    path "versions.yml"                   , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
+    def arriba_version = '2.5.0'
     """
-    wget https://github.com/suhrig/arriba/releases/download/v2.4.0/arriba_v2.4.0.tar.gz -O arriba_v2.4.0.tar.gz --no-check-certificate
-    tar -xzvf arriba_v2.4.0.tar.gz
-    rm arriba_v2.4.0.tar.gz
-    mv arriba_v2.4.0/database/* .
-    rm -r arriba_v2.4.0
+    wget https://github.com/suhrig/arriba/releases/download/v${arriba_version}/arriba_v${arriba_version}.tar.gz -O arriba_v${arriba_version}.tar.gz --no-check-certificate
+    tar -xzvf arriba_v${arriba_version}.tar.gz
+    rm arriba_v${arriba_version}.tar.gz
+    mv arriba_v${arriba_version}/database/* .
+    rm -r arriba_v${arriba_version}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
@@ -35,11 +36,12 @@ process ARRIBA_DOWNLOAD {
     """
 
     stub:
+    def arriba_version = '2.5.0'
     """
-    touch blacklist_hg38_GRCh38_v2.4.0.tsv.gz
-    touch protein_domains_hg38_GRCh38_v2.4.0.gff3
-    touch cytobands_hg38_GRCh38_v2.4.0.tsv
-    touch known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
+    echo | gzip > blacklist_hg38_GRCh38_v${arriba_version}.tsv.gz
+    touch protein_domains_hg38_GRCh38_v${arriba_version}.gff3
+    touch cytobands_hg38_GRCh38_v${arriba_version}.tsv
+    echo | gzip > known_fusions_hg38_GRCh38_v${arriba_version}.tsv.gz
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
index d9e28cc06..06a8ef60d 100644
--- a/modules/nf-core/arriba/download/meta.yml
+++ b/modules/nf-core/arriba/download/meta.yml
@@ -16,37 +16,44 @@ tools:
       identifier: biotools:Arriba
 
 input:
-  - - genome:
-        type: string
-        description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
+  - genome:
+      type: string
+      description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
 output:
-  - blacklist:
-      - blacklist*${genome}*.tsv.gz:
-          type: string
-          description: The blacklist removes recurrent alignment artifacts and transcripts
-            which are present in healthy tissue
-          pattern: ".tsv.gz"
-  - cytobands:
-      - cytobands*${genome}*.tsv:
-          type: file
-          description: Coordinates of the Giemsa staining bands. This information is used
-            to draw ideograms
-          pattern: ".tsv"
-  - protein_domains:
-      - protein_domains*${genome}*.gff3:
-          type: file
-          description: Protein domain annotations
-          pattern: "*.gff3"
-  - known_fusions:
-      - known_fusions*${genome}*.tsv.gz:
-          type: file
-          description: Arriba is more sensitive to those fusions to improve the detection
-            rate of expected or highly relevant events, such as recurrent fusions
-          pattern: "*.tsv.gz"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+  blacklist:
+    - blacklist*${genome}*.tsv.gz:
+        type: string
+        description: The blacklist removes recurrent alignment artifacts and transcripts
+          which are present in healthy tissue
+        pattern: ".tsv.gz"
+  cytobands:
+    - cytobands*${genome}*.tsv:
+        type: file
+        description: Coordinates of the Giemsa staining bands. This information is used
+          to draw ideograms
+        pattern: ".tsv"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+  protein_domains:
+    - protein_domains*${genome}*.gff3:
+        type: file
+        description: Protein domain annotations
+        pattern: "*.gff3"
+        ontologies: []
+  known_fusions:
+    - known_fusions*${genome}*.tsv.gz:
+        type: file
+        description: Arriba is more sensitive to those fusions to improve the detection
+          rate of expected or highly relevant events, such as recurrent fusions
+        pattern: "*.tsv.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@peterpru"
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
index d12a38bbf..ec25745a7 100644
--- a/modules/nf-core/arriba/download/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/download/tests/main.nf.test.snap
@@ -3,41 +3,41 @@
         "content": [
             {
                 "0": [
-                    "blacklist_hg38_GRCh38_v2.4.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
+                    "blacklist_hg38_GRCh38_v2.5.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
                 ],
                 "1": [
-                    "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
+                    "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
                 ],
                 "2": [
-                    "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
+                    "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
                 ],
                 "3": [
-                    "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
+                    "known_fusions_hg38_GRCh38_v2.5.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
                 ],
                 "4": [
-                    "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+                    "versions.yml:md5,2457cdc32bc582854616aba806a91d35"
                 ],
                 "blacklist": [
-                    "blacklist_hg38_GRCh38_v2.4.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
+                    "blacklist_hg38_GRCh38_v2.5.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
                 ],
                 "cytobands": [
-                    "cytobands_hg38_GRCh38_v2.4.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
+                    "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
                 ],
                 "known_fusions": [
-                    "known_fusions_hg38_GRCh38_v2.4.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
+                    "known_fusions_hg38_GRCh38_v2.5.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
                 ],
                 "protein_domains": [
-                    "protein_domains_hg38_GRCh38_v2.4.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
+                    "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
                 ],
                 "versions": [
-                    "versions.yml:md5,98c69df5eaea5caf0b4af7b8d7af4893"
+                    "versions.yml:md5,2457cdc32bc582854616aba806a91d35"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-12-16T01:46:32.110653034"
+        "timestamp": "2025-07-14T11:54:10.203072"
     }
 }
\ No newline at end of file

From b3bbb7929acc6508416952ac258360f2557108a6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 15 Jul 2025 13:50:11 +0200
Subject: [PATCH 616/887] handle no fusion cases

---
 subworkflows/local/fusioninspector_workflow.nf | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index f33d2a86f..5da212aac 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -42,7 +42,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         FUSIONINSPECTOR( ch_reads_fusion, ch_starfusion_ref)
         ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
 
-        if(!skip_vcf) {
+        if(!skip_vcf && !FUSIONINSPECTOR.out.tsv[1].isEmpty() && !FUSIONINSPECTOR.out.out_gtf[1].isEmpty()) {
             AGAT_CONVERTSPGFF2TSV(FUSIONINSPECTOR.out.out_gtf)
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
@@ -50,9 +50,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)
-        }
 
-        if (!skip_vis) {
             ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(FUSIONINSPECTOR.out.tsv)
             ARRIBA_VISUALISATION(
                 ch_bam_sorted_indexed_fusions,
@@ -62,6 +60,8 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             )
             ch_versions = ch_versions.mix(ARRIBA_VISUALISATION.out.versions)
             ch_arriba_visualisation = ARRIBA_VISUALISATION.out.pdf
+        } else {
+            log.warn("FUSIONINSPECTOR confirmed no fusions, skipping VCF and visualisation steps.")
         }
 
     emit:

From 4c1269d38d34665b84a2a98a266d8073c19af273 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 15 Jul 2025 14:56:41 +0200
Subject: [PATCH 617/887] update fusioncatcher download

---
 conf/modules.config                          |  2 +-
 modules/local/fusioncatcher/download/main.nf | 10 +++++-----
 2 files changed, 6 insertions(+), 6 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 95331646b..777e7fb22 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -208,7 +208,7 @@ process {
     }
 
 
-    withName:PICARD_COLLECTRNASEQMETRICS {
+    withName: 'PICARD_COLLECTRNASEQMETRICS' {
         ext.args = { ( meta.strandedness == "forward" || meta.single_end ) ?
             "--STRAND_SPECIFICITY FIRST_READ_TRANSCRIPTION_STRAND" :
             meta.strandedness == "reverse" ?
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 2cc34f052..7c2de381c 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -4,8 +4,8 @@ process FUSIONCATCHER_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3b/3b54fa9135194c72a18d00db6b399c03248103f87e43ca75e4b50d61179994b3/data':
-        'community.wave.seqera.io/library/wget:1.21.4--8b0fcde81c17be5e' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b97801b2147f99c974bdf01b821c043654f6bf1f2864a1633231740999df072/data':
+        'community.wave.seqera.io/library/tar_pip_gdown:34715940085a0f1c' }"
 
     input:
     val genome_gencode_version
@@ -22,13 +22,13 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    wget --no-check-certificate $args ${params.fusioncatcher_download_link}
+    gdown $args ${params.fusioncatcher_download_link}
     tar xz human_v${genome_gencode_version}.tar.gz
     rm human_v${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
+        gdown: \$(gdown --version | head -1 | cut -d ' ' -f 2)
         tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """
@@ -39,7 +39,7 @@ process FUSIONCATCHER_DOWNLOAD {
     touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
+        gdown: \$(gdown --version | head -1 | cut -d ' ' -f 2)
         tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """

From 1c00a6e5e0840b3b4c2ac113e7b42a0dda6b1182 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Tue, 15 Jul 2025 20:19:16 +0000
Subject: [PATCH 618/887] Add option for custom dfam urls or automatical
 filling

---
 nextflow.config                               | 10 +++----
 subworkflows/local/build_references/main.nf   | 26 ++++++++++++++-----
 .../utils_nfcore_rnafusion_pipeline/main.nf   |  8 +++++-
 tests/test_build.nf.test                      |  2 +-
 4 files changed, 32 insertions(+), 14 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index c75e9b31f..7cd0b5dfe 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -85,11 +85,11 @@ params {
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
     ctatsplicing_cancer_introns   = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
-    dfam_hmm                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"
-    dfam_h3f                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f"
-    dfam_h3i                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i"
-    dfam_h3m                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3,"
-    dfam_h3p                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p"
+    dfam_hmm                      = null
+    dfam_h3f                      = null
+    dfam_h3i                      = null
+    dfam_h3m                      = null
+    dfam_h3p                      = null
     pfam_url                      = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"
     annot_filter_url              = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
 
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 083a0e093..b8e3e6473 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -171,13 +171,25 @@ workflow BUILD_REFERENCES {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
             }
-            dfam_urls_ch = Channel.of([
-                params.dfam_hmm,
-                params.dfam_h3f,
-                params.dfam_h3i,
-                params.dfam_h3m,
-                params.dfam_h3p
-            ])
+            if(params.dfam_hmm && params.dfam_h3p && params.dfam_h3m && params.dfam_h3i && params.dfam_h3f) {
+                dfam_hmm = Channel.of(params.dfam_hmm)
+                dfam_h3f = Channel.of(params.dfam_h3f)
+                dfam_h3i = Channel.of(params.dfam_h3i)
+                dfam_h3m = Channel.of(params.dfam_h3m)
+                dfam_h3p = Channel.of(params.dfam_h3p)
+            } else {
+                dfam_hmm = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm")
+                dfam_h3f = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f")
+                dfam_h3i = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i")
+                dfam_h3m = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3")
+                dfam_h3p = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p")
+            }
+            dfam_urls_ch = dfam_hmm
+                .concat(dfam_h3f,
+                dfam_h3i,
+                dfam_h3m,
+                dfam_h3p
+                ).collect().view()
             STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, params.pfam_url, dfam_urls_ch, params.annot_filter_url)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 7921f82bf..8d2657ab4 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -64,7 +64,9 @@ workflow PIPELINE_INITIALISATION {
     //
     // Custom validation for pipeline parameters
     //
-    validateInputParameters()
+    validateInputParameters(
+
+    )
 
     //
     // Create channel from input file provided through params.input
@@ -162,6 +164,10 @@ def validateInputParameters() {
         log.warn("Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`")
     }
 
+    if ((params.dfam_hmm || params.dfam_h3p || params.dfam_h3m || params.dfam_h3i || params.dfam_h3f) && (params.dfam_version)) {
+        log.warn("Both custom dfam_urls and dfam_version were specified. \n If you want to use custom dfam URLs make sure to provide the full paths for each of the dfam params as the dfam_version will not be overwritten. \n Otherwise, use only the `--dfam_version` and `--species` params and the dfam URLs will be automatically filled.")
+    }
+
 }
 
 //
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 6fb5cc32a..57653836a 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -20,7 +20,7 @@ nextflow_pipeline {
             // Ignore files with timestamps in their names
             def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
             // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore', 'PFAM.domtblout.dat.gz', 'blast_pairs.*', '')
             assertAll(
                 { assert workflow.success},
                 { assert snapshot(

From f9b21b99a1d3957f9f593b37decd9ffce2f31fc7 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 15 Jul 2025 22:47:11 +0000
Subject: [PATCH 619/887] style: fix linting issue

---
 CHANGELOG.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5270e02f0..631b45ca0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -114,7 +114,6 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed subworkflow with less than two modules: `STARFUSION_WORKFLOW` [#707](https://github.com/nf-core/rnafusion/pull/707)
 - Removed subworkflow with less than two modules: `CTATSPLICING_WORKFLOW` [#704](https://github.com/nf-core/rnafusion/pull/704)
 
-
 ### Parameters
 
 | Old parameter         | New parameter                   |

From 092a59493853356dd21d562aa3599217cddb96b5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 09:04:52 +0200
Subject: [PATCH 620/887] update fusionreport

---
 modules.json                                  |  4 +-
 modules/nf-core/fusionreport/detect/main.nf   |  2 +-
 modules/nf-core/fusionreport/detect/meta.yml  | 44 +++++++++----------
 .../fusionreport/detect/tests/main.nf.test    | 12 ++---
 .../fusionreport/download/environment.yml     | 32 +++++++-------
 modules/nf-core/fusionreport/download/main.nf |  2 +-
 .../download/tests/main.nf.test.snap          |  6 +--
 7 files changed, 48 insertions(+), 54 deletions(-)

diff --git a/modules.json b/modules.json
index 0999e9124..8a5322cb0 100644
--- a/modules.json
+++ b/modules.json
@@ -47,12 +47,12 @@
                     },
                     "fusionreport/detect": {
                         "branch": "master",
-                        "git_sha": "412d1e0614c3be267b8feb448588864a16a48293",
+                        "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
                         "installed_by": ["modules"]
                     },
                     "fusionreport/download": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
                         "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
diff --git a/modules/nf-core/fusionreport/detect/main.nf b/modules/nf-core/fusionreport/detect/main.nf
index 0254100f5..618c14504 100644
--- a/modules/nf-core/fusionreport/detect/main.nf
+++ b/modules/nf-core/fusionreport/detect/main.nf
@@ -4,7 +4,7 @@ process FUSIONREPORT_DETECT {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'oras://community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:353260d00d0da0ec' :
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ed/ed32f46746a5b33a1b5d597cfe2f62d3b1cfa54638f57cadc5d5158d6a4959d2/data' :
         'community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:78488bd99166aa9a'}"
 
     input:
diff --git a/modules/nf-core/fusionreport/detect/meta.yml b/modules/nf-core/fusionreport/detect/meta.yml
index 9d28add25..dc87feed9 100644
--- a/modules/nf-core/fusionreport/detect/meta.yml
+++ b/modules/nf-core/fusionreport/detect/meta.yml
@@ -45,13 +45,13 @@ input:
         type: directory
         description: File
         pattern: "fusion_report_db"
-  - - tools_cutoff:
-        type: integer
-        description: Discard fusions detected by less than $tools_cutoff tools both for display in fusionreport html index and to consider in fusioninspector.
+  - tools_cutoff:
+      type: integer
+      description: Discard fusions detected by less than $tools_cutoff tools both for display in fusionreport html index and to consider in fusioninspector.
 
 output:
-  - fusion_list:
-      - meta:
+  fusion_list:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -65,8 +65,8 @@ output:
           pattern: "*.tsv"
           ontologies:
             - edam: http://edamontology.org/format_3475 # TSV
-  - fusion_list_filtered:
-      - meta:
+  fusion_list_filtered:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -80,8 +80,8 @@ output:
           pattern: "*.tsv"
           ontologies:
             - edam: http://edamontology.org/format_3475 # TSV
-  - report:
-      - meta:
+  report:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -95,8 +95,8 @@ output:
           pattern: "*.index.html"
           ontologies:
             - edam: "http://edamontology.org/format_2331" # HTML
-  - html:
-      - meta:
+  html:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -110,8 +110,8 @@ output:
           pattern: "*_*.html"
           ontologies:
             - edam: "http://edamontology.org/format_2331" # HTML
-  - csv:
-      - meta:
+  csv:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -125,8 +125,8 @@ output:
           pattern: "*.csv"
           ontologies:
             - edam: "http://edamontology.org/format_3752" # CSV
-  - json:
-      - meta:
+  json:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -140,12 +140,12 @@ output:
           pattern: "*.json"
           ontologies:
             - edam: "http://edamontology.org/format_3464" # JSON
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-          ontologies:
-            - edam: http://edamontology.org/format_3750 # YAML
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@praveenraj2018, @rannick"
diff --git a/modules/nf-core/fusionreport/detect/tests/main.nf.test b/modules/nf-core/fusionreport/detect/tests/main.nf.test
index 3ff9b6877..baf1ce12f 100644
--- a/modules/nf-core/fusionreport/detect/tests/main.nf.test
+++ b/modules/nf-core/fusionreport/detect/tests/main.nf.test
@@ -27,14 +27,12 @@ nextflow_process {
         when {
             process {
                 """
-
                 input[0] = [
                     [ id:'test_sample' ], // meta map
-                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv"),
-                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv"),
-                    file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt")
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/arriba.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/starfusion.tsv', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/fusioncatcher.txt', checkIfExists: true)
                 ]
-
                 input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
                 input[2] = 1
                 """
@@ -86,18 +84,14 @@ nextflow_process {
         when {
             process {
                 """
-
                 input[0] = [
                     [ id:'test_sample' ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/arriba.tsv', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/starfusion.tsv', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/fusioncatcher.txt', checkIfExists: true)
                 ]
-
                 input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
-
                 input[2] = 1
-
                 """
             }
         params {
diff --git a/modules/nf-core/fusionreport/download/environment.yml b/modules/nf-core/fusionreport/download/environment.yml
index ae86909d1..59bbfc643 100644
--- a/modules/nf-core/fusionreport/download/environment.yml
+++ b/modules/nf-core/fusionreport/download/environment.yml
@@ -1,18 +1,18 @@
 channels:
-  - conda-forge
-  - bioconda
+- conda-forge
+- bioconda
 dependencies:
-  - bioconda::fusion-report=4.0.1
-  - conda-forge::beautifulsoup4=4.13.4
-  - conda-forge::click=8.2.1
-  - conda-forge::colorlog=6.9.0
-  - conda-forge::jinja2=3.1.6
-  - conda-forge::markupsafe=3.0.2
-  - conda-forge::numpy=2.3.0
-  - conda-forge::openpyxl=3.1.5
-  - conda-forge::pandas=2.3.0
-  - conda-forge::pyyaml=6.0.2
-  - conda-forge::requests=2.32.4
-  - conda-forge::tabulate=0.9.0
-  - conda-forge::tqdm=4.67.1
-  - conda-forge::xlrd=2.0.1
+- bioconda::fusion-report=4.0.1
+- conda-forge::beautifulsoup4=4.13.4
+- conda-forge::click=8.2.1
+- conda-forge::colorlog=6.9.0
+- conda-forge::jinja2=3.1.6
+- conda-forge::markupsafe=3.0.2
+- conda-forge::numpy=2.3.0
+- conda-forge::openpyxl=3.1.5
+- conda-forge::pandas=2.3.0
+- conda-forge::pyyaml=6.0.2
+- conda-forge::requests=2.32.4
+- conda-forge::tabulate=0.9.0
+- conda-forge::tqdm=4.67.1
+- conda-forge::xlrd=2.0.1
diff --git a/modules/nf-core/fusionreport/download/main.nf b/modules/nf-core/fusionreport/download/main.nf
index a74a5f2eb..3fd7ada4a 100644
--- a/modules/nf-core/fusionreport/download/main.nf
+++ b/modules/nf-core/fusionreport/download/main.nf
@@ -4,7 +4,7 @@ process FUSIONREPORT_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'oras://community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:353260d00d0da0ec' :
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ed/ed32f46746a5b33a1b5d597cfe2f62d3b1cfa54638f57cadc5d5158d6a4959d2/data' :
         'community.wave.seqera.io/library/fusion-report_beautifulsoup4_click_colorlog_pruned:78488bd99166aa9a'}"
 
     output:
diff --git a/modules/nf-core/fusionreport/download/tests/main.nf.test.snap b/modules/nf-core/fusionreport/download/tests/main.nf.test.snap
index ab161336a..ae2d0bc85 100644
--- a/modules/nf-core/fusionreport/download/tests/main.nf.test.snap
+++ b/modules/nf-core/fusionreport/download/tests/main.nf.test.snap
@@ -2,7 +2,7 @@
     "Download fusionreport databases": {
         "content": [
             "fusiongdb2.db:md5,9dec75bfc146f56b36e5a84358f9e515",
-            "mitelman.db:md5,d31478ed66bd6623172852d94132e933",
+            "mitelman.db:md5,c271894c1cd62ba2735a060411c16dea",
             true,
             true,
             [
@@ -11,9 +11,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.4"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-30T15:25:09.631428688"
+        "timestamp": "2025-07-15T18:56:45.064154384"
     },
     "Create stub files": {
         "content": [

From 034876f31c3fff2fdb7d9207965a9bf712fdf9cc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 09:42:24 +0200
Subject: [PATCH 621/887] update fusioninspector

---
 modules/local/fusioninspector/main.nf | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 54a546983..95ab06735 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -14,7 +14,7 @@ process FUSIONINSPECTOR {
     output:
     tuple val(meta), path("*FusionInspector.fusions.tsv")                  , emit: tsv
     tuple val(meta), path("fi_workdir/*.gtf")                              , optional:true, emit: out_gtf
-    tuple val(meta), path("*FusionInspector.log")                          , emit: log
+    tuple val(meta), path("FusionInspector.log")                           , emit: log
     tuple val(meta), path("*html")                                         , emit: html
     tuple val(meta), path("*abridged.tsv")                                 , emit: abridged_tsv
     tuple val(meta), path("IGV_inputs")                                    , emit: igv_inputs
@@ -48,6 +48,7 @@ process FUSIONINSPECTOR {
     mkdir -p IGV_inputs
     mkdir -p fi_workdir
     mkdir -p chckpts_dir
+    touch fi_workdir/${prefix}.gtf
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From b5da338a92ba3df08549aff32f4a8f112982ae75 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 09:43:50 +0200
Subject: [PATCH 622/887] update ctatsplicing snap

---
 .../prepgenomelib/tests/main.nf.test.snap     | 46 +++++++++----------
 1 file changed, 23 insertions(+), 23 deletions(-)

diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
index b0fa56d6d..ed1df4107 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
@@ -8,8 +8,8 @@
                             "id": "minigenome fasta"
                         },
                         [
-                            "AnnotFilterRule.pm:md5,5391fcc58d9c71cd1f0e45668c5ec597",
-                            "PFAM.domtblout.dat.gz:md5,26c8d24b6cd736eef6b5d2ddaf337672",
+                            "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
+                            "PFAM.domtblout.dat.gz:md5,b8cf97310b4af1275237febab723d16c",
                             [
                                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -35,42 +35,42 @@
                                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
                             ],
-                            "blast_pairs.dat.gz:md5,3e7fe3a6fa372742b8574940abdc9a37",
-                            "blast_pairs.idx:md5,b8a9567c9f2b8f099a95956e7dadfdd5",
+                            "blast_pairs.dat.gz:md5,31ec48d5116f44b12fdd3f47155241dc",
+                            "blast_pairs.idx:md5,0264ed1a28c50370e598cc8bd874dce0",
                             [
-                                "cancer_splicing.idx:md5,712867939a7c235bdc47dd3fdb03e014"
+                                "cancer_splicing.idx:md5,fcec006060ebd0bfb1b0cf38c9f78116"
                             ],
                             "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                            "fusion_annot_lib.idx:md5,d77513b8a65344e32f976cb9df54e0c4",
-                            "pfam_domains.dbm:md5,03f514fe96a42d54eef7429eb7bd2216",
-                            "refGene.bed:md5,d854402131f44d84a14cd90f912f886d",
+                            "fusion_annot_lib.idx:md5,21bc9b75123be07089e86023285b67f8",
+                            "pfam_domains.dbm:md5,68a00826b188aa577237a701e6f18e8c",
+                            "refGene.bed:md5,a14d9396c44c5fc0cd6b4d3194e8902d",
                             "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
                             "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
-                            "ref_annot.cdna.fa:md5,a912c9684f1f14b266ff8f6ee6b16b6c",
-                            "ref_annot.cdna.fa.idx:md5,8555d23ba563a84c34a55e9a7b1b14c9",
-                            "ref_annot.cds:md5,19c35508510e3cbd13d70e586712ff71",
-                            "ref_annot.cdsplus.fa:md5,cee60e0eed67964731618f2cb39eb509",
-                            "ref_annot.cdsplus.fa.idx:md5,555823919c78863b03505ed793299562",
+                            "ref_annot.cdna.fa:md5,4907484edddb1a52d1ef5acb814fa709",
+                            "ref_annot.cdna.fa.idx:md5,8f5c6a6c2ffec0188fac2483f806aabc",
+                            "ref_annot.cds:md5,de204070512c909b351c5876133f4f54",
+                            "ref_annot.cdsplus.fa:md5,180f4e5d277f2d5fc4bb092c11eaaf68",
+                            "ref_annot.cdsplus.fa.idx:md5,be8bb33932b6c2f82213a0622241ad0f",
                             "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                            "ref_annot.gtf.gene_spans:md5,20f0a837edf800511c65436f9f5372b6",
+                            "ref_annot.gtf.gene_spans:md5,4884ab6d453096b48bf85595299d9183",
                             "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                             "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                            "ref_annot.pep:md5,655d2d2955149bf632e7ba7036bbc01c",
-                            "ref_annot.prot_info.dbm:md5,6298fed0de42c97c668e3fb8ac4d5e20",
+                            "ref_annot.pep:md5,22f05c0bf182e2645f0a01c4fb45e908",
+                            "ref_annot.prot_info.dbm:md5,218a49ac07f0db5fba0b1b48e6b12343",
                             "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                             "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                             "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                             "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                             "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                            "ref_genome.fa.nin:md5,2317a90875fe530dff8e2b95bc8bbee1",
-                            "ref_genome.fa.njs:md5,3127dd8e9e5897924974328a359e383d",
+                            "ref_genome.fa.nin:md5,3c4114ca4fb5dd0dc2095f4cf5f7b844",
+                            "ref_genome.fa.njs:md5,39b423551f658bb0cb5032ac00bd74eb",
                             "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                             "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                             "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                             "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                             [
                                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                                "Log.out:md5,f0d53c14008b8a936d65a458ac01345f",
+                                "Log.out:md5,5310b905153fa779043520ec3fc7ca3b",
                                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -81,14 +81,14 @@
                                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                                "genomeParameters.txt:md5,767f208cbd11c22bcb315b0254b0d9de",
+                                "genomeParameters.txt:md5,9664cf5658a941c5d4cf9f5bb5e5cb4f",
                                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2"
                             ],
                             "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                            "trans.blast.align_coords.align_coords.dbm:md5,02f34eb24b0888e0d6adafd4860fafb9",
+                            "trans.blast.align_coords.align_coords.dbm:md5,4ae2e5e8d141bd2e066da74ffb60403b",
                             "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
                         ]
                     ]
@@ -100,9 +100,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-03-19T10:53:52.549180977"
+        "timestamp": "2025-07-16T09:41:16.195052"
     },
     "test - stub": {
         "content": [

From e6519aff6e7f19c6ad1527958f193caed41fdb55 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 09:54:35 +0200
Subject: [PATCH 623/887] update arriba vis snap

---
 .../arriba/visualisation/tests/main.nf.test.snap   | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/modules/local/arriba/visualisation/tests/main.nf.test.snap b/modules/local/arriba/visualisation/tests/main.nf.test.snap
index 7ed24df79..37b4aead2 100644
--- a/modules/local/arriba/visualisation/tests/main.nf.test.snap
+++ b/modules/local/arriba/visualisation/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "versions": {
         "content": [
             [
-                "versions.yml:md5,77b78715defd458bd9e742e58e39cd42"
+                "versions.yml:md5,6f45b05e1e9ad4830266e897f3ee41b7"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-03-11T19:05:38.906558079"
+        "timestamp": "2025-07-16T09:51:22.731321"
     },
     "ARRIBA_VISUALISATION stub": {
         "content": [
@@ -24,7 +24,7 @@
                     ]
                 ],
                 "1": [
-                    "versions.yml:md5,77b78715defd458bd9e742e58e39cd42"
+                    "versions.yml:md5,6f45b05e1e9ad4830266e897f3ee41b7"
                 ],
                 "pdf": [
                     [
@@ -36,14 +36,14 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,77b78715defd458bd9e742e58e39cd42"
+                    "versions.yml:md5,6f45b05e1e9ad4830266e897f3ee41b7"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-03-11T19:05:48.424051167"
+        "timestamp": "2025-07-16T09:51:53.819687"
     }
 }
\ No newline at end of file

From 5385242b6b793b76a1fd3cd2b5f78a7d1661a472 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 10:12:21 +0200
Subject: [PATCH 624/887] update fusioncatcher download link

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 0a0ea5596..7e135f39f 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -64,7 +64,7 @@ params {
 
     // Download references option
     download_refs               = false
-    fusioncatcher_download_link = "https://drive.google.com/file/d/1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo/view?usp=drive_link"
+    fusioncatcher_download_link = "1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo"
 
     // Path to references
     fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"

From 233bc0e005e5fa3747036498a14c2230e8a39959 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 10:18:22 +0200
Subject: [PATCH 625/887] update fusioncatcher download link pattern check

---
 nextflow_schema.json | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index a0794cd62..81bfd8c2e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -159,8 +159,7 @@
                 "fusioncatcher_download_link": {
                     "type": "string",
                     "description": "Fusioncatcher download link",
-                    "fa_icon": "far fa-file-code",
-                    "pattern": "^https?://.*$"
+                    "fa_icon": "far fa-file-code"
                 },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",

From 94eacdbcf26a3227ae6a3ee6f887d5f928d8a9dd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 12:27:51 +0200
Subject: [PATCH 626/887] update logical gate and snap fusioninsp

---
 .../local/fusioninspector_workflow.nf         |   9 +-
 tests/test_stub.nf.test.snap                  |   2 +-
 tests/test_stub_bam.nf.test.snap              | 461 +-----------------
 3 files changed, 14 insertions(+), 458 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index 5da212aac..873732146 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -42,7 +42,14 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         FUSIONINSPECTOR( ch_reads_fusion, ch_starfusion_ref)
         ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
 
-        if(!skip_vcf && !FUSIONINSPECTOR.out.tsv[1].isEmpty() && !FUSIONINSPECTOR.out.out_gtf[1].isEmpty()) {
+        def tsv_nonempty = FUSIONINSPECTOR.out.tsv.filter { meta, file -> file.exists() && file.size() > 0 }
+        def gtf_nonempty = FUSIONINSPECTOR.out.out_gtf.filter { meta, file -> file.exists() && file.size() > 0 }
+
+        if (
+            !skip_vcf &&
+            tsv_nonempty &&
+            gtf_nonempty
+        ) {
             AGAT_CONVERTSPGFF2TSV(FUSIONINSPECTOR.out.out_gtf)
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 181adc772..3622ff5c7 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -877,7 +877,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T16:00:28.831228"
+        "timestamp": "2025-07-16T12:24:40.524114"
     },
     "stub test salmon": {
         "content": [
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 4a3dd8c85..0efca7371 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -775,7 +775,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T16:53:36.788101"
+        "timestamp": "2025-07-16T12:27:08.644076"
     },
     "stub test salmon": {
         "content": [
@@ -1515,467 +1515,16 @@
     },
     "stub test all": {
         "content": [
-            35,
-            {
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.1"
-                },
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.5.0"
-                },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.5.0"
-                },
-                "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "CTATSPLICING_STARTOCANCERINTRONS": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "FASTP": {
-                    "fastp": "0.24.0"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "FASTQC_FOR_FASTP": {
-                    "fastqc": "0.12.1"
-                },
-                "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
-                },
-                "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
-                },
-                "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
-                },
-                "FUSIONREPORT_DETECT": {
-                    "fusion_report": "4.0.1"
-                },
-                "FUSIONREPORT_DOWNLOAD": {
-                    "fusion_report": "4.0.1"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GFFREAD": {
-                    "gffread": "0.12.7"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "HGNC_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SALMON_INDEX": {
-                    "salmon": "1.10.3"
-                },
-                "SALMON_QUANT": {
-                    "salmon": "1.10.3"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
-                "STARFUSION_BUILD": {
-                    "wget": "1.21.4",
-                    "STAR-Fusion": "1.15.0"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
-                "STRINGTIE_MERGE": {
-                    "stringtie": "2.2.1"
-                },
-                "STRINGTIE_STRINGTIE": {
-                    "stringtie": "2.2.3"
-                },
-                "VCF_COLLECT": {
-                    "python": "3.11.0"
-                },
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
+            0,
+            null,
             [
-                "agat",
-                "agat/test.tsv",
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
-                "ctatsplicing",
-                "ctatsplicing/test.cancer.introns",
-                "ctatsplicing/test.cancer.introns.prelim",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/test.chckpts",
-                "ctatsplicing/test.ctat-splicing.igv.html",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/test.igv.tracks",
-                "ctatsplicing/test.introns",
-                "ctatsplicing/test.introns.for_IGV.bed",
-                "fastp",
-                "fastp/test.fastp.html",
-                "fastp/test.fastp.json",
-                "fastp/test.fastp.log",
-                "fastp/test_1.fastp.fastq.gz",
-                "fastp/test_2.fastp.fastq.gz",
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
-                "fusioncatcher",
-                "fusioncatcher/test.fusion-genes.txt",
-                "fusioncatcher/test.log",
-                "fusioncatcher/test.summary.txt",
-                "fusioninspector",
-                "fusioninspector/test",
-                "fusioninspector/test/FusionInspector.log",
-                "fusioninspector/test/IGV_inputs",
-                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/test/IGV_inputs/cytoBand.txt",
-                "fusioninspector/test/IGV_inputs/test.ROI.bed",
-                "fusioninspector/test/IGV_inputs/test.bed",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.fa",
-                "fusioninspector/test/IGV_inputs/test.fa.fai",
-                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/test/IGV_inputs/test.gtf",
-                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/tracks.json",
-                "fusioninspector/test/chckpts_dir",
-                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/test/chckpts_dir/blast_filter.ok",
-                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/test/chckpts_dir/cp_final.ok",
-                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/test/chckpts_dir/cytoband.ok",
-                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/test/chckpts_dir/final.abridged.ok",
-                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/test/chckpts_dir/microH.dat.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/test/chckpts_dir/run_STAR.ok",
-                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
-                "fusioninspector/test/fi_workdir",
-                "fusioninspector/test/fi_workdir/Log.final.out",
-                "fusioninspector/test/fi_workdir/Log.out",
-                "fusioninspector/test/fi_workdir/Log.progress.out",
-                "fusioninspector/test/fi_workdir/SJ.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/test/fi_workdir/microH.dat",
-                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/test/fi_workdir/star_align.ok",
-                "fusioninspector/test/fi_workdir/test.fa",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
-                "fusioninspector/test/fi_workdir/test.gtf",
-                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
-                "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
-                "fusioninspector/test/test.FusionInspector.fusions.tsv",
-                "fusioninspector/test/test.fusion_inspector_web.html",
-                "fusionreport",
-                "fusionreport/test",
-                "fusionreport/test/AAA_BBB.html",
-                "fusionreport/test/test.fusionreport.tsv",
-                "fusionreport/test/test.fusionreport_filtered.tsv",
-                "fusionreport/test/test.fusions.csv",
-                "fusionreport/test/test.fusions.json",
-                "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
-                "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
-                "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
-                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/refGene.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
-                "salmon",
-                "salmon/test",
-                "salmon/test_lib_format_counts.json",
-                "salmon/test_meta_info.json",
-                "star",
-                "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai",
-                "starfusion",
-                "starfusion/test.starfusion.abridged.coding_effect.tsv",
-                "starfusion/test.starfusion.abridged.tsv",
-                "starfusion/test.starfusion.fusion_predictions.tsv",
-                "stringtie",
-                "stringtie/[:]",
-                "stringtie/[:]/stringtie.merged.gtf",
-                "stringtie/test.ballgown",
-                "stringtie/test.coverage.gtf",
-                "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf",
-                "vcf",
-                "vcf/test_fusion_data.vcf"
+                "pipeline_info"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T16:09:39.371336"
+        "timestamp": "2025-07-16T10:17:16.677957"
     }
 }
\ No newline at end of file

From d53f43afeda00b7ccd71b5e62a6510962e81397d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 12:30:53 +0200
Subject: [PATCH 627/887] auto confirm

---
 nextflow.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/nextflow.config b/nextflow.config
index 7e135f39f..792bde099 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -64,7 +64,7 @@ params {
 
     // Download references option
     download_refs               = false
-    fusioncatcher_download_link = "1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo"
+    fusioncatcher_download_link = "1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo&confirm=t"
 
     // Path to references
     fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"

From a909212256fdd3ab2261b84d2782e7a93109c1cb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 12:57:02 +0200
Subject: [PATCH 628/887] update stringtie workflow

---
 .../stringtie_workflow/tests/main.nf.test.snap     | 14 +++++++-------
 1 file changed, 7 insertions(+), 7 deletions(-)

diff --git a/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap b/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
index 791c85e41..7d59d5476 100644
--- a/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
@@ -33,24 +33,24 @@
                     "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
                 ],
                 "1": [
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
                     "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
                 ],
                 "stringtie_gtf": [
                     "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
                 ],
                 "versions": [
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
                     "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.1"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-11-27T17:50:57.437102535"
+        "timestamp": "2025-07-16T12:33:08.507844"
     }
 }
\ No newline at end of file

From b4b2cffe1b41929c5fea573a5a082ca08399caa9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 13:36:27 +0200
Subject: [PATCH 629/887] update version stringtie snap

---
 modules/local/fusioncatcher/download/main.nf       |  3 +--
 .../stringtie_workflow/tests/main.nf.test.snap     | 14 +++++++-------
 2 files changed, 8 insertions(+), 9 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 7c2de381c..e22ca05f4 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,8 +22,7 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    gdown $args ${params.fusioncatcher_download_link}
-    tar xz human_v${genome_gencode_version}.tar.gz
+    gdown $args ${params.fusioncatcher_download_link} | tar xz human_v${genome_gencode_version}.tar.gz
     rm human_v${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap b/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
index 7d59d5476..2abc99913 100644
--- a/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
@@ -6,25 +6,25 @@
                     "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
                 ],
                 "1": [
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
                     "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
                 ],
                 "stringtie_gtf": [
                     "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
                 ],
                 "versions": [
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
-                    "versions.yml:md5,16bf76d2f56399280213cfa721195317",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
+                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
                     "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.1"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-11-27T17:51:10.73612885"
+        "timestamp": "2025-07-16T12:58:20.587179"
     },
     "Should run stringtie workflow with stringtie = true": {
         "content": [

From e5dda65390fa2511cff5ab8e3d24b4dbc8dc0243 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 13:46:51 +0200
Subject: [PATCH 630/887] remove cosmic test

---
 tests/test_cosmic.nf.test      | 101 ---------------------------------
 tests/test_cosmic.nf.test.snap |  58 -------------------
 2 files changed, 159 deletions(-)
 delete mode 100644 tests/test_cosmic.nf.test
 delete mode 100644 tests/test_cosmic.nf.test.snap

diff --git a/tests/test_cosmic.nf.test b/tests/test_cosmic.nf.test
deleted file mode 100644
index b547b2fe4..000000000
--- a/tests/test_cosmic.nf.test
+++ /dev/null
@@ -1,101 +0,0 @@
-nextflow_pipeline {
-
-    name "Test pipeline"
-    script "../main.nf"
-    profile "test_cosmic"
-    tag "pipeline"
-    tag "pipeline_rnafusion"
-    tag "test_cosmic"
-
-    test("test cosmic with fastp trim") {
-
-        when {
-            params {
-                outdir               = "$outputDir"
-                tools                = "fastp"
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-
-    test("test cosmic no fastp trim") {
-
-        when {
-            params {
-                outdir     = "$outputDir"
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-
-    test("test cosmic no fastp trim build") {
-
-        when {
-            params {
-                outdir           = "$outputDir"
-                build_references = true
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            // Ignore files with timestamps in their names
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-}
diff --git a/tests/test_cosmic.nf.test.snap b/tests/test_cosmic.nf.test.snap
deleted file mode 100644
index c920e0321..000000000
--- a/tests/test_cosmic.nf.test.snap
+++ /dev/null
@@ -1,58 +0,0 @@
-{
-    "test cosmic no fastp trim build": {
-        "content": [
-            0,
-            {
-                "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
-                }
-            },
-            [
-                "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"
-            ],
-            [
-
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-10T12:42:49.558429"
-    },
-    "test cosmic no fastp trim": {
-        "content": [
-            0,
-            null,
-            [
-                "pipeline_info"
-            ],
-            [
-
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-10T15:09:53.130295"
-    },
-    "test cosmic with fastp trim": {
-        "content": [
-            0,
-            null,
-            [
-                "pipeline_info"
-            ],
-            [
-
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-10T15:09:29.277399"
-    }
-}

From 5a0ee3a96fb3f7601889e9b34f5c9c0a854a1135 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 14:05:34 +0200
Subject: [PATCH 631/887] update tests

---
 tests/test_stub_bam.nf.test.snap | 459 ++++++++++++++++++++++++++++++-
 1 file changed, 455 insertions(+), 4 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 0efca7371..19b5897c0 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -1515,16 +1515,467 @@
     },
     "stub test all": {
         "content": [
-            0,
-            null,
+            35,
+            {
+                "AGAT_CONVERTSPGFF2TSV": {
+                    "agat": "v1.4.1"
+                },
+                "ARRIBA_ARRIBA": {
+                    "arriba": "2.5.0"
+                },
+                "ARRIBA_DOWNLOAD": {
+                    "arriba_download": "2.5.0"
+                },
+                "ARRIBA_VISUALISATION": {
+                    "arriba": "2.5.0"
+                },
+                "CTATSPLICING_PREPGENOMELIB": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "CTATSPLICING_STARTOCANCERINTRONS": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "FASTP": {
+                    "fastp": "0.24.0"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "FASTQC_FOR_FASTP": {
+                    "fastqc": "0.12.1"
+                },
+                "FUSIONCATCHER_DOWNLOAD": {
+                    "wget": "1.21.4",
+                    "tar": 1.35
+                },
+                "FUSIONCATCHER_FUSIONCATCHER": {
+                    "fusioncatcher": "1.33"
+                },
+                "FUSIONINSPECTOR": {
+                    "STAR-Fusion": null
+                },
+                "FUSIONREPORT_DETECT": {
+                    "fusion_report": "4.0.1"
+                },
+                "FUSIONREPORT_DOWNLOAD": {
+                    "fusion_report": "4.0.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GET_RRNA_TRANSCRIPTS": {
+                    "get_rrna_transcripts": "v1.0"
+                },
+                "GFFREAD": {
+                    "gffread": "0.12.7"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "HGNC_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SALMON_INDEX": {
+                    "salmon": "1.10.3"
+                },
+                "SALMON_QUANT": {
+                    "salmon": "1.10.3"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION": {
+                    "STAR-Fusion": "1.15.0"
+                },
+                "STARFUSION_BUILD": {
+                    "wget": "1.21.4",
+                    "STAR-Fusion": "1.15.0"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "STRINGTIE_MERGE": {
+                    "stringtie": "2.2.1"
+                },
+                "STRINGTIE_STRINGTIE": {
+                    "stringtie": "2.2.3"
+                },
+                "VCF_COLLECT": {
+                    "python": "3.11.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
             [
-                "pipeline_info"
+                "agat",
+                "agat/test.tsv",
+                "arriba",
+                "arriba/test.arriba.fusions.discarded.tsv",
+                "arriba/test.arriba.fusions.tsv",
+                "arriba_visualisation",
+                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
+                "ctatsplicing",
+                "ctatsplicing/test.cancer.introns",
+                "ctatsplicing/test.cancer.introns.prelim",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/test.chckpts",
+                "ctatsplicing/test.ctat-splicing.igv.html",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/test.igv.tracks",
+                "ctatsplicing/test.introns",
+                "ctatsplicing/test.introns.for_IGV.bed",
+                "fastp",
+                "fastp/test.fastp.html",
+                "fastp/test.fastp.json",
+                "fastp/test.fastp.log",
+                "fastp/test_1.fastp.fastq.gz",
+                "fastp/test_2.fastp.fastq.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
+                "fusioncatcher",
+                "fusioncatcher/test.fusion-genes.txt",
+                "fusioncatcher/test.log",
+                "fusioncatcher/test.summary.txt",
+                "fusioninspector",
+                "fusioninspector/test",
+                "fusioninspector/test/FusionInspector.log",
+                "fusioninspector/test/IGV_inputs",
+                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
+                "fusioninspector/test/IGV_inputs/cytoBand.txt",
+                "fusioninspector/test/IGV_inputs/test.ROI.bed",
+                "fusioninspector/test/IGV_inputs/test.bed",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
+                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
+                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.fa",
+                "fusioninspector/test/IGV_inputs/test.fa.fai",
+                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
+                "fusioninspector/test/IGV_inputs/test.gtf",
+                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
+                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
+                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
+                "fusioninspector/test/IGV_inputs/tracks.json",
+                "fusioninspector/test/chckpts_dir",
+                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
+                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
+                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
+                "fusioninspector/test/chckpts_dir/blast_filter.ok",
+                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
+                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
+                "fusioninspector/test/chckpts_dir/cp_final.ok",
+                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
+                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
+                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
+                "fusioninspector/test/chckpts_dir/cytoband.ok",
+                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
+                "fusioninspector/test/chckpts_dir/final.abridged.ok",
+                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
+                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
+                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
+                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
+                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
+                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
+                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
+                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
+                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
+                "fusioninspector/test/chckpts_dir/microH.dat.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
+                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
+                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
+                "fusioninspector/test/chckpts_dir/run_STAR.ok",
+                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
+                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
+                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
+                "fusioninspector/test/fi_workdir",
+                "fusioninspector/test/fi_workdir/Log.final.out",
+                "fusioninspector/test/fi_workdir/Log.out",
+                "fusioninspector/test/fi_workdir/Log.progress.out",
+                "fusioninspector/test/fi_workdir/SJ.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
+                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
+                "fusioninspector/test/fi_workdir/microH.dat",
+                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
+                "fusioninspector/test/fi_workdir/star_align.ok",
+                "fusioninspector/test/fi_workdir/test.fa",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
+                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
+                "fusioninspector/test/fi_workdir/test.gtf",
+                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
+                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
+                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
+                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
+                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
+                "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
+                "fusioninspector/test/test.FusionInspector.fusions.tsv",
+                "fusioninspector/test/test.fusion_inspector_web.html",
+                "fusionreport",
+                "fusionreport/test",
+                "fusionreport/test/AAA_BBB.html",
+                "fusionreport/test/test.fusionreport.tsv",
+                "fusionreport/test/test.fusionreport_filtered.tsv",
+                "fusionreport/test/test.fusions.csv",
+                "fusionreport/test/test.fusions.json",
+                "fusionreport/test/test_fusionreport_index.html",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "get",
+                "get/rrna.bed",
+                "get/rrna.gtf",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/arriba",
+                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
+                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
+                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
+                "references/fusion_report_db",
+                "references/fusion_report_db/DB-timestamp.txt",
+                "references/fusion_report_db/cosmic.db",
+                "references/fusion_report_db/fusion_report.log",
+                "references/fusion_report_db/fusiongdb2.db",
+                "references/fusion_report_db/mitelman.db",
+                "references/fusioncatcher",
+                "references/fusioncatcher/human_v46",
+                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
+                "references/gencode",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/gffread",
+                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/hgnc",
+                "references/hgnc/HGNC-DB-timestamp.txt",
+                "references/hgnc/hgnc_complete_set.txt",
+                "references/salmon",
+                "references/salmon/salmon",
+                "references/salmon/salmon/complete_ref_lens.bin",
+                "references/salmon/salmon/ctable.bin",
+                "references/salmon/salmon/ctg_offsets.bin",
+                "references/salmon/salmon/duplicate_clusters.tsv",
+                "references/salmon/salmon/info.json",
+                "references/salmon/salmon/mphf.bin",
+                "references/salmon/salmon/pos.bin",
+                "references/salmon/salmon/pre_indexing.log",
+                "references/salmon/salmon/rank.bin",
+                "references/salmon/salmon/refAccumLengths.bin",
+                "references/salmon/salmon/ref_indexing.log",
+                "references/salmon/salmon/reflengths.bin",
+                "references/salmon/salmon/refseq.bin",
+                "references/salmon/salmon/seq.bin",
+                "references/salmon/salmon/versionInfo.json",
+                "references/star",
+                "references/star/Genome",
+                "references/star/Log.out",
+                "references/star/SA",
+                "references/star/SAindex",
+                "references/star/chrLength.txt",
+                "references/star/chrName.txt",
+                "references/star/chrNameLength.txt",
+                "references/star/chrStart.txt",
+                "references/star/exonGeTrInfo.tab",
+                "references/star/exonInfo.tab",
+                "references/star/geneInfo.tab",
+                "references/star/genomeParameters.txt",
+                "references/star/sjdbInfo.txt",
+                "references/star/sjdbList.fromGTF.out.tab",
+                "references/star/sjdbList.out.tab",
+                "references/star/transcriptInfo.tab",
+                "references/starfusion",
+                "references/starfusion/ctat_genome_lib_build_dir",
+                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf",
+                "vcf",
+                "vcf/test_fusion_data.vcf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-16T10:17:16.677957"
+        "timestamp": "2025-07-16T13:01:46.880339"
     }
 }
\ No newline at end of file

From 5ea8679af71eff58abf2c4b7ecc4d22629c210b8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 14:15:21 +0200
Subject: [PATCH 632/887] update tests

---
 modules/local/starfusion/detect/tests/main.nf.test | 2 +-
 tests/test_stub.nf.test.snap                       | 8 ++++----
 2 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
index a01a60f9f..72b8cac6d 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test
+++ b/modules/local/starfusion/detect/tests/main.nf.test
@@ -19,7 +19,7 @@ nextflow_process {
                 ]
 
                 input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                sinput [3] = "homo_sapiens"
+                input [3] = "homo_sapiens"
                 input [4] = "3.8"
                 input [5] = "37.4"
                 """
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 181adc772..627503a92 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1620,8 +1620,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1687,9 +1687,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-19T11:19:49.213204"
+        "timestamp": "2025-07-16T14:10:47.772811"
     },
     "stub test all": {
         "content": [

From bb8a1df1b41bb8cf0c31de7fad29fe5136239619 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 15:05:19 +0200
Subject: [PATCH 633/887] update link and download process

---
 modules/local/fusioncatcher/download/main.nf | 3 ++-
 nextflow.config                              | 2 +-
 2 files changed, 3 insertions(+), 2 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index e22ca05f4..7c2de381c 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,7 +22,8 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    gdown $args ${params.fusioncatcher_download_link} | tar xz human_v${genome_gencode_version}.tar.gz
+    gdown $args ${params.fusioncatcher_download_link}
+    tar xz human_v${genome_gencode_version}.tar.gz
     rm human_v${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
diff --git a/nextflow.config b/nextflow.config
index 792bde099..7e135f39f 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -64,7 +64,7 @@ params {
 
     // Download references option
     download_refs               = false
-    fusioncatcher_download_link = "1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo&confirm=t"
+    fusioncatcher_download_link = "1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo"
 
     // Path to references
     fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"

From a0a40767a239dda6f95658388e260995b7b0ddec Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 15:15:49 +0200
Subject: [PATCH 634/887] update fusionreport

---
 modules.json                                  |  2 +-
 .../fusionreport/download/environment.yml     | 32 +++++++++----------
 2 files changed, 17 insertions(+), 17 deletions(-)

diff --git a/modules.json b/modules.json
index 8a5322cb0..d8c39f79e 100644
--- a/modules.json
+++ b/modules.json
@@ -52,7 +52,7 @@
                     },
                     "fusionreport/download": {
                         "branch": "master",
-                        "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
+                        "git_sha": "df4b662dae05afb695a4326fc172178b5a7a48ce",
                         "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
diff --git a/modules/nf-core/fusionreport/download/environment.yml b/modules/nf-core/fusionreport/download/environment.yml
index 59bbfc643..ae86909d1 100644
--- a/modules/nf-core/fusionreport/download/environment.yml
+++ b/modules/nf-core/fusionreport/download/environment.yml
@@ -1,18 +1,18 @@
 channels:
-- conda-forge
-- bioconda
+  - conda-forge
+  - bioconda
 dependencies:
-- bioconda::fusion-report=4.0.1
-- conda-forge::beautifulsoup4=4.13.4
-- conda-forge::click=8.2.1
-- conda-forge::colorlog=6.9.0
-- conda-forge::jinja2=3.1.6
-- conda-forge::markupsafe=3.0.2
-- conda-forge::numpy=2.3.0
-- conda-forge::openpyxl=3.1.5
-- conda-forge::pandas=2.3.0
-- conda-forge::pyyaml=6.0.2
-- conda-forge::requests=2.32.4
-- conda-forge::tabulate=0.9.0
-- conda-forge::tqdm=4.67.1
-- conda-forge::xlrd=2.0.1
+  - bioconda::fusion-report=4.0.1
+  - conda-forge::beautifulsoup4=4.13.4
+  - conda-forge::click=8.2.1
+  - conda-forge::colorlog=6.9.0
+  - conda-forge::jinja2=3.1.6
+  - conda-forge::markupsafe=3.0.2
+  - conda-forge::numpy=2.3.0
+  - conda-forge::openpyxl=3.1.5
+  - conda-forge::pandas=2.3.0
+  - conda-forge::pyyaml=6.0.2
+  - conda-forge::requests=2.32.4
+  - conda-forge::tabulate=0.9.0
+  - conda-forge::tqdm=4.67.1
+  - conda-forge::xlrd=2.0.1

From 7703d6a7099d1777a01d1279025866dc7612ecdf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 15:46:09 +0200
Subject: [PATCH 635/887] add fuzzy option

---
 modules/local/fusioncatcher/download/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index 7c2de381c..f2ac07ee5 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -22,7 +22,7 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    gdown $args ${params.fusioncatcher_download_link}
+    gdown $args --fuzzy ${params.fusioncatcher_download_link}
     tar xz human_v${genome_gencode_version}.tar.gz
     rm human_v${genome_gencode_version}.tar*
 

From 750afa131d2c9e1ce56a4d7c79379d67b741b894 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 16 Jul 2025 15:51:44 +0200
Subject: [PATCH 636/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 36b9a7534..71e156f4f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -103,6 +103,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Upgrade STAR-Fusion to 1.15.1 to solve problem building STAR-Fusion references with `--human_gencode_filter` [#683](https://github.com/nf-core/rnafusion/pull/683)
 - Fix missing memory unit for fusioncatcher [#674](https://github.com/nf-core/rnafusion/pull/674)
 - Fix fusioncatcher download link [#693](https://github.com/nf-core/rnafusion/pull/693)
+- Fix fusionreport singularity container [#713](https://github.com/nf-core/rnafusion/pull/713)
 
 ### Removed
 

From 867a341456b98bc46392e37d58e40851163b0a47 Mon Sep 17 00:00:00 2001
From: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 16 Jul 2025 11:33:51 -0300
Subject: [PATCH 637/887] Update workflows/rnafusion.nf

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 3087fbdcb..603371ac8 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -256,7 +256,7 @@ workflow RNAFUSION {
 
             if (params.starfusion_fusions) {
                 def fusions = file(params.starfusion_fusions, checkIfExists:true)                   // TODO: What is fusions used for? Remove?
-                ch_starfusion_fusions = ch_star_junctions.map { meta, _junc -> [ meta, fusions ] }  // TODO: We use the junctions but not the provided fusions
+                ch_starfusion_fusions = ch_star_junctions.map { meta, _junc -> [ meta, fusions ] }
             } else {
                 STARFUSION(
                     ch_star_junctions.map { meta, junc -> [ meta, [], junc ] },

From 29b930a28933e30102927dc6fe45db291a862da2 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 16 Jul 2025 14:34:58 +0000
Subject: [PATCH 638/887] style: remove comment

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 603371ac8..0d4666aed 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -255,7 +255,7 @@ workflow RNAFUSION {
             fusions_created = true
 
             if (params.starfusion_fusions) {
-                def fusions = file(params.starfusion_fusions, checkIfExists:true)                   // TODO: What is fusions used for? Remove?
+                def fusions = file(params.starfusion_fusions, checkIfExists:true)
                 ch_starfusion_fusions = ch_star_junctions.map { meta, _junc -> [ meta, fusions ] }
             } else {
                 STARFUSION(

From f490444bd8b4e782d6d20d4e28337c681997e65a Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Wed, 16 Jul 2025 17:18:20 +0000
Subject: [PATCH 639/887] Add option for custom or automatical filling of pfam
 and dfam URLs

---
 conf/test_build.config                      |  2 +-
 nextflow.config                             |  6 +-
 nextflow_schema.json                        | 61 ++++++++++++++++++++-
 subworkflows/local/build_references/main.nf | 43 +++++++++------
 4 files changed, 90 insertions(+), 22 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index a483ed658..3ba2891b1 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -43,7 +43,7 @@ params {
 
     fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
     species                    = 'homo_sapiens'
-    dfam_version               = '3.4'
+    dfam_version               = 3.4
 
 }
 
diff --git a/nextflow.config b/nextflow.config
index 7cd0b5dfe..1cf2c0437 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -32,8 +32,8 @@ params {
     genomes                    = [:]
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
     species                    = "homo_sapiens"
-    dfam_version               = '3.8'
-    pfam_version               = '37.4'
+    dfam_version               = 3.8
+    pfam_version               = 37.4
 
     // Filtering
     tools_cutoff               = 1
@@ -90,7 +90,7 @@ params {
     dfam_h3i                      = null
     dfam_h3m                      = null
     dfam_h3p                      = null
-    pfam_url                      = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"
+    pfam_url                      = null
     annot_filter_url              = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
 
     // Path to fusion outputs
diff --git a/nextflow_schema.json b/nextflow_schema.json
index a472d333f..f51e2be17 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -231,6 +231,63 @@
                     "description": "Path to the cancer introns CSV file to create the CTAT-SPLICING reference with",
                     "default": "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
                 },
+                "dfam_hmm": {
+                    "type": "string",
+                    "format": "uri",
+                    "pattern": "^https?://.*",
+                    "description": "URL to Dfam HMM database file",
+                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam HMM database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "fa_icon": "fas fa-link"
+                },
+                "dfam_h3f": {
+                    "type": "string",
+                    "format": "uri",
+                    "pattern": "^https?://.*",
+                    "description": "URL to Dfam H3F database file",
+                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3F database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "fa_icon": "fas fa-link"
+                },
+                "dfam_h3i": {
+                    "type": "string",
+                    "format": "uri",
+                    "pattern": "^https?://.*",
+                    "description": "URL to Dfam H3I database file",
+                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3I database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "fa_icon": "fas fa-link"
+                },
+                "dfam_h3m": {
+                    "type": "string",
+                    "format": "uri",
+                    "pattern": "^https?://.*",
+                    "description": "URL to Dfam H3M database file",
+                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3M database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "fa_icon": "fas fa-link"
+                },
+                "dfam_h3p": {
+                    "type": "string",
+                    "format": "uri",
+                    "pattern": "^https?://.*",
+                    "description": "URL to Dfam H3P database file",
+                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3P database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "fa_icon": "fas fa-link"
+                },
+                "pfam_url": {
+                    "type": "string",
+                    "format": "uri",
+                    "pattern": "^https?://.*",
+                    "description": "URL to Pfam database file",
+                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Pfam database file. Leave as null if using the automatical filling with `--pfam_version`.",
+                    "fa_icon": "fas fa-link"
+                },
+                "annot_filter_url": {
+                    "type": "string",
+                    "format": "uri",
+                    "pattern": "^https?://.*",
+                    "description": "URL to annotation filter rule file",
+                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the annotation filter rule file (typically a Perl module).",
+                    "default": "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm",
+                    "fa_icon": "fas fa-filter"
+                },
                 "starindex_ref": {
                     "type": "string",
                     "format": "directory-path",
@@ -368,11 +425,11 @@
                     "default": "homo_sapiens"
                 },
                 "dfam_version": {
-                    "type": "string",
+                    "type": "number",
                     "description": "Version of dfam to use"
                 },
                 "pfam_version": {
-                    "type": "string",
+                    "type": "number",
                     "description": "Version of pfam to use"
                 }
             }
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index b8e3e6473..852185390 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -171,26 +171,37 @@ workflow BUILD_REFERENCES {
             if(!params.fusion_annot_lib) {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
             }
+
+            if(params.pfam_url) {
+                pfam_url = Channel.fromPath(params.pfam_url, checkIfExists: true)
+            } else {
+                pfam_url = Channel.fromPath("http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz", checkIfExists: true)
+            }
+
             if(params.dfam_hmm && params.dfam_h3p && params.dfam_h3m && params.dfam_h3i && params.dfam_h3f) {
-                dfam_hmm = Channel.of(params.dfam_hmm)
-                dfam_h3f = Channel.of(params.dfam_h3f)
-                dfam_h3i = Channel.of(params.dfam_h3i)
-                dfam_h3m = Channel.of(params.dfam_h3m)
-                dfam_h3p = Channel.of(params.dfam_h3p)
+                dfam_hmm = Channel.fromPath(params.dfam_hmm, checkIfExists: true)
+                dfam_h3f = Channel.fromPath(params.dfam_h3f, checkIfExists: true)
+                dfam_h3i = Channel.fromPath(params.dfam_h3i, checkIfExists: true)
+                dfam_h3m = Channel.fromPath(params.dfam_h3m, checkIfExists: true)
+                dfam_h3p = Channel.fromPath(params.dfam_h3p, checkIfExists: true)
             } else {
-                dfam_hmm = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm")
-                dfam_h3f = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f")
-                dfam_h3i = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i")
-                dfam_h3m = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3")
-                dfam_h3p = Channel.of("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p")
+                dfam_hmm = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"    , checkIfExists: true)
+                dfam_h3f = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f", checkIfExists: true)
+                dfam_h3i = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i", checkIfExists: true)
+                dfam_h3m = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m", checkIfExists: true)
+                dfam_h3p = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p", checkIfExists: true)
             }
+
             dfam_urls_ch = dfam_hmm
-                .concat(dfam_h3f,
-                dfam_h3i,
-                dfam_h3m,
-                dfam_h3p
-                ).collect().view()
-            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, params.pfam_url, dfam_urls_ch, params.annot_filter_url)
+                .concat(
+                    dfam_h3f,
+                    dfam_h3i,
+                    dfam_h3m,
+                    dfam_h3p
+                )
+                .collect()
+
+            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, pfam_url, dfam_urls_ch, params.annot_filter_url)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
                 CTATSPLICING_PREPGENOMELIB(

From 803d884052840425acbcf37804104f80c9a01cf9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:03:15 +0200
Subject: [PATCH 640/887] use wget with drive

---
 modules/local/fusioncatcher/download/main.nf | 12 +++++++-----
 1 file changed, 7 insertions(+), 5 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index f2ac07ee5..c1f204fc1 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -4,8 +4,8 @@ process FUSIONCATCHER_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b97801b2147f99c974bdf01b821c043654f6bf1f2864a1633231740999df072/data':
-        'community.wave.seqera.io/library/tar_pip_gdown:34715940085a0f1c' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3b/3b54fa9135194c72a18d00db6b399c03248103f87e43ca75e4b50d61179994b3/data':
+        'community.wave.seqera.io/library/wget:1.21.4--8b0fcde81c17be5e' }"
 
     input:
     val genome_gencode_version
@@ -22,13 +22,15 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    gdown $args --fuzzy ${params.fusioncatcher_download_link}
+    wget --no-check-certificate \\
+        "https://drive.google.com/uc?export=download&id=${params.fusioncatcher_download_link}" \\
+        -O human_v46.tar.gz
     tar xz human_v${genome_gencode_version}.tar.gz
     rm human_v${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        gdown: \$(gdown --version | head -1 | cut -d ' ' -f 2)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
         tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """
@@ -39,7 +41,7 @@ process FUSIONCATCHER_DOWNLOAD {
     touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        gdown: \$(gdown --version | head -1 | cut -d ' ' -f 2)
+        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
         tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """

From f2804c6b435467fbf72ebb8f3fe490904963760c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:06:09 +0200
Subject: [PATCH 641/887] update snap

---
 tests/test_stub.nf.test.snap | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 627503a92..e450622b3 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1620,8 +1620,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "gdown": "5.2.0",
-                    "tar": 1.34
+                    "wget": "1.21.4",
+                    "tar": 1.35
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1689,7 +1689,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-16T14:10:47.772811"
+        "timestamp": "2025-07-17T10:05:52.553051"
     },
     "stub test all": {
         "content": [

From d20dd0e97d9de7a8e0137cfb1bebb3c2a698a2a7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:32:24 +0200
Subject: [PATCH 642/887] re-test with gdown

---
 modules/local/fusioncatcher/download/main.nf | 12 +++++-------
 1 file changed, 5 insertions(+), 7 deletions(-)

diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
index c1f204fc1..8a3d1a306 100644
--- a/modules/local/fusioncatcher/download/main.nf
+++ b/modules/local/fusioncatcher/download/main.nf
@@ -4,8 +4,8 @@ process FUSIONCATCHER_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3b/3b54fa9135194c72a18d00db6b399c03248103f87e43ca75e4b50d61179994b3/data':
-        'community.wave.seqera.io/library/wget:1.21.4--8b0fcde81c17be5e' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b97801b2147f99c974bdf01b821c043654f6bf1f2864a1633231740999df072/data':
+        'community.wave.seqera.io/library/tar_pip_gdown:34715940085a0f1c' }"
 
     input:
     val genome_gencode_version
@@ -22,15 +22,13 @@ process FUSIONCATCHER_DOWNLOAD {
     def args = task.ext.args ?: ''
     meta = [ id: "human_v${genome_gencode_version}" ]
     """
-    wget --no-check-certificate \\
-        "https://drive.google.com/uc?export=download&id=${params.fusioncatcher_download_link}" \\
-        -O human_v46.tar.gz
+    gdown --no-cookies ${params.fusioncatcher_download_link} -O human_v46.tar.gz
     tar xz human_v${genome_gencode_version}.tar.gz
     rm human_v${genome_gencode_version}.tar*
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
+        gdown: \$(gdown --version | head -1 | cut -d ' ' -f 2)
         tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """
@@ -41,7 +39,7 @@ process FUSIONCATCHER_DOWNLOAD {
     touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
+        gdown: \$(gdown --version | head -1 | cut -d ' ' -f 2)
         tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
     END_VERSIONS
     """

From 70bfcf640276056829c8a08a38f6d67e18181054 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:34:15 +0200
Subject: [PATCH 643/887] remove default test

---
 tests/default.nf.test | 35 -----------------------------------
 1 file changed, 35 deletions(-)
 delete mode 100644 tests/default.nf.test

diff --git a/tests/default.nf.test b/tests/default.nf.test
deleted file mode 100644
index 0d7279dc0..000000000
--- a/tests/default.nf.test
+++ /dev/null
@@ -1,35 +0,0 @@
-nextflow_pipeline {
-
-    name "Test pipeline"
-    script "../main.nf"
-    tag "pipeline"
-
-    test("-profile test") {
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // Number of successful tasks
-                    workflow.trace.succeeded().size(),
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we test pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-}

From 2c0c55770cca042f05f8c921cc2b2d2dc4d561f6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:43:01 +0200
Subject: [PATCH 644/887] remove default test

---
 .nf-core.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index 9b7277aa0..61d6e6bef 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,6 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
+    - tests/default.nf.test
 nf_core_version: 3.3.2
 repository_type: pipeline
 template:

From a68647931c897477bf3ec5824483c951e7ac47ff Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:48:01 +0200
Subject: [PATCH 645/887] update ignoring removal of default test

---
 .nf-core.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.nf-core.yml b/.nf-core.yml
index 61d6e6bef..5f86f8416 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -4,6 +4,7 @@ lint:
     - .github/PULL_REQUEST_TEMPLATE.md
     - conf/igenomes.config
     - conf/igenomes_ignored.config
+  files_exist:
     - tests/default.nf.test
 nf_core_version: 3.3.2
 repository_type: pipeline

From f4cbe8e381057ddb33fefe36332f0f894209065c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:58:19 +0200
Subject: [PATCH 646/887] update logo

---
 docs/images/logo.svg                          | 19439 ++++++++++++++++
 .../nf-core-rnafusion_logo_dark_new.png       |   Bin 0 -> 390124 bytes
 .../nf-core-rnafusion_logo_light_new.png      |   Bin 0 -> 357944 bytes
 3 files changed, 19439 insertions(+)
 create mode 100644 docs/images/logo.svg
 create mode 100644 docs/images/nf-core-rnafusion_logo_dark_new.png
 create mode 100644 docs/images/nf-core-rnafusion_logo_light_new.png

diff --git a/docs/images/logo.svg b/docs/images/logo.svg
new file mode 100644
index 000000000..6885517f7
--- /dev/null
+++ b/docs/images/logo.svg
@@ -0,0 +1,19439 @@
+<?xml version="1.0" encoding="UTF-8" standalone="no"?>
+<!-- Created with Inkscape (http://www.inkscape.org/) -->
+
+<svg
+   width="210mm"
+   height="297mm"
+   viewBox="0 0 210 297"
+   version="1.1"
+   id="svg5"
+   inkscape:export-filename="/Users/annick.renevey/Desktop/logo/test1.png"
+   inkscape:export-xdpi="300"
+   inkscape:export-ydpi="300"
+   inkscape:version="1.1.2 (b8e25be8, 2022-02-05)"
+   sodipodi:docname="logo.svg"
+   xmlns:inkscape="http://www.inkscape.org/namespaces/inkscape"
+   xmlns:sodipodi="http://sodipodi.sourceforge.net/DTD/sodipodi-0.dtd"
+   xmlns:xlink="http://www.w3.org/1999/xlink"
+   xmlns="http://www.w3.org/2000/svg"
+   xmlns:svg="http://www.w3.org/2000/svg">
+  <sodipodi:namedview
+     id="namedview7"
+     pagecolor="#ffffff"
+     bordercolor="#666666"
+     borderopacity="1.0"
+     inkscape:pageshadow="2"
+     inkscape:pageopacity="0.0"
+     inkscape:pagecheckerboard="0"
+     inkscape:document-units="mm"
+     showgrid="false"
+     inkscape:zoom="0.47267396"
+     inkscape:cx="773.26028"
+     inkscape:cy="291.956"
+     inkscape:window-width="1920"
+     inkscape:window-height="1056"
+     inkscape:window-x="0"
+     inkscape:window-y="24"
+     inkscape:window-maximized="0"
+     inkscape:current-layer="layer1" />
+  <defs
+     id="defs2" />
+  <g
+     inkscape:label="Layer 1"
+     inkscape:groupmode="layer"
+     id="layer1">
+    <image
+       width="75.266998"
+       height="41.901218"
+       preserveAspectRatio="none"
+       style="image-rendering:optimizeQuality"
+       xlink:href="data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAADCAAAAbACAYAAAAM9MaLAAAAUGVYSWZNTQAqAAAACAACARIAAwAA
+AAEAAQAAh2kABAAAAAEAAAAmAAAAAAADoAEAAwAAAAEAAQAAoAIABAAAAAEAAAMIoAMABAAAAAEA
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+nhKZkanKJZKxcXGn0zfbDTtU9Z55oQtUYTA3MjIYvvD/Y8DNAOhyAZegHIqc5x5zdwEAAAAAAAAA
+AAAAAAAAAAAAALxNeNcLAAAAAAAAAAAAAAAAAAAAAAAA7z8CCAAAAAAAAAAAAAAAAAAAAAAA4F4E
+EAAAAAAAAAAAAAAAAAAAAAAAwL0IIAAAAAAAAAAAAAAAAAAAAAAAgHsRQAAAAAAAAAAAAAAAAAAA
+AAAAAPcigAAAAAAAAAAAAAAAAAAAAAAAAO5FAAEAAAAAAAAAAAAAAAAAAAAAANyLAAIAAAAAAAAA
+AAAAAAAAAAAAALgXAQQAAAAAAAAAAAAAAAAAAAAAAHAvAggAAAAAAAAAAAAAAAAAAAAAAOBeBBAA
+AAAAAAAAAAAAAAAAAAAAAMC9CCAAAAAAAAAAAAAAAAAAAAAAAIB7EUAAAAAAAAAAAAAAAAAAAAAA
+AAD3IoAAAAAAAAAAAAAAAAAAAAAAAADuRQABAAAAAAAAAAAAAAAAAAAAAADciwACAAAAAAAAAAAA
+AAAAAAAAAAC4FwEEAAAAAAAAAAAAAAAAAAAAAABwLwIIAAAAAAAAAAAAAAAAAAAAAADgXgQQAAAA
+AAAAAAAAAAAAAAAAAADAvQggAAAAAAAAAAAAAAAAAAAAAACAexFAAAAAAAAAAAAAAAAAAAAAAAAA
+9yKAAAAAAAAAAAAAAAAAAAAAAAAA7kUAAQAAAAAAAAAAAAAAAAAAAAAA3IsAAgAAAAAAAAAAAAAA
+AAAAAAAAuBcBBAAAAAAAAAAAAAAAAAAAAAAAcC8CCAAAAAAAAAAAAAAAAAAAAAAA4F4EEAAAAAAA
+AAAAAAAAAAAAAAAAwL0IIAAAAAAAAAAAAAAAAAAAAAAAgHsRQAAAAAAAAAAAAAAAAAAAAAAAAPci
+gAAAAAAAAAAAAAAAAAAAAAAAAO5FAAEAAAAAAAAAAAAAAAAAAAAAANyLAAIAAAAAAAAAAAAAAAAA
+AAAAALgXAQQAAAAAAAAAAAAAAAAAAAAAAHAvAggAAAAAAAAAAAAAAAAAAAAAAOBeBBAAAAAAAAAA
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From 8cf9ad8fd88d1b45e79876db1feb960417975660 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 10:59:43 +0200
Subject: [PATCH 647/887] use new logo

---
 README.md              | 4 ++--
 ro-crate-metadata.json | 2 +-
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/README.md b/README.md
index c1a3ca7d5..0943e2a53 100644
--- a/README.md
+++ b/README.md
@@ -1,7 +1,7 @@
 <h1>
   <picture>
-    <source media="(prefers-color-scheme: dark)" srcset="docs/images/nf-core-rnafusion_logo_dark.png">
-    <img alt="nf-core/rnafusion" src="docs/images/nf-core-rnafusion_logo_light.png">
+    <source media="(prefers-color-scheme: dark)" srcset="docs/images/nf-core-rnafusion_logo_dark_new.png">
+    <img alt="nf-core/rnafusion" src="docs/images/nf-core-rnafusion_logo_light_new.png">
   </picture>
 </h1>
 
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index ccf7d6388..aef6f6d69 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-04-30T12:27:54+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was originally written by Martin Proks, Annick Renevey.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"

From a5c87d313bbeaadc1c84229ef9312c95fd80bfbd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 11:13:56 +0200
Subject: [PATCH 648/887] update logo dimension

---
 .../nf-core-rnafusion_logo_dark_new.png       | Bin 390124 -> 108683 bytes
 .../nf-core-rnafusion_logo_light_new.png      | Bin 357944 -> 83081 bytes
 2 files changed, 0 insertions(+), 0 deletions(-)

diff --git a/docs/images/nf-core-rnafusion_logo_dark_new.png b/docs/images/nf-core-rnafusion_logo_dark_new.png
index ae0f2ac9ccaaf0908c3bfc2e24a99e8860d2cd12..c79d6e1e1448ea48bcb0ebc62da06e99e2ccb0a5 100644
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HMa2ICbZWu6


From b6c60c30adb01154969a7493d1439e1e901cf6c5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 11:18:31 +0200
Subject: [PATCH 649/887] update name

---
 docs/images/{logo.svg => rnafusion_logo_new.svg} | 0
 1 file changed, 0 insertions(+), 0 deletions(-)
 rename docs/images/{logo.svg => rnafusion_logo_new.svg} (100%)

diff --git a/docs/images/logo.svg b/docs/images/rnafusion_logo_new.svg
similarity index 100%
rename from docs/images/logo.svg
rename to docs/images/rnafusion_logo_new.svg

From ae357ec734891bb260b5b74412997ddef8801897 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 13:38:07 +0200
Subject: [PATCH 650/887] update arriba refs params

---
 nextflow.config | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index f747a9116..60e9b8c21 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -73,9 +73,9 @@ params {
     refflat                       = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf.refflat"
     rrna_intervals                = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.interval_list"
     no_cosmic                     = false
-    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv"
+    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
     arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
-    arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv"
+    arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
     arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
     fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}"
     hgnc_ref                      = "${params.genomes_base}/hgnc/hgnc_complete_set.txt"

From de2541e33c9b6ad2cb6e5d11c868b4b69d21a7f3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 13:38:15 +0200
Subject: [PATCH 651/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7d3747b09..cf836fe3c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -104,6 +104,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix missing memory unit for fusioncatcher [#674](https://github.com/nf-core/rnafusion/pull/674)
 - Fix fusioncatcher download link [#693](https://github.com/nf-core/rnafusion/pull/693)
 - Fix fusionreport singularity container [#713](https://github.com/nf-core/rnafusion/pull/713)
+- Fix behavior when no fusion is present (log a warning and avoid running depending processes)[#714](https://github.com/nf-core/rnafusion/pull/714)
 
 ### Removed
 

From 43a8ebd672ce2fe3b3e8733fbceaf9056255470a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 13:50:26 +0200
Subject: [PATCH 652/887] update schema

---
 nextflow_schema.json | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index a0794cd62..d902733f0 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -97,7 +97,7 @@
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "format": "file-path",
-                    "pattern": "\\S+\\.tsv$",
+                    "pattern": "\\S+\\.tsv.gz$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
                 },
@@ -111,7 +111,7 @@
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "format": "file-path",
-                    "pattern": "\\S+\\.tsv$",
+                    "pattern": "\\S+\\.tsv.gz$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference known fusions"
                 },

From d299b3b95bdba64cc9a9466a247d86503ebcac9f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 14:10:38 +0200
Subject: [PATCH 653/887] changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7d3747b09..4383082d3 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -84,6 +84,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Using FusionInspector abridged output (that now contains coding effects) for downstream analysis [#669](https://github.com/nf-core/rnafusion/pull/669)
 - Replaced local `FUSIONREPORT` and `FUSIONREPORT_DOWNLOAD` with `FUSIONREPORT_DETECT` and `FUSIONREPORT_DOWNLOAD` from nf-core [#703](https://github.com/nf-core/rnafusion/pull/703)
 - Updated arriba to v2.5.0 [#693](https://github.com/nf-core/rnafusion/pull/693)
+- Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
 
 ### Fixed
 

From 3025198bfd0c7f9ee1703d5324585387713cdf4b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 14:19:34 +0200
Subject: [PATCH 654/887] add a clean up disk space step

---
 .github/workflows/nf-test.yml | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index e7b58449b..9ec706eca 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -89,6 +89,9 @@ jobs:
         with:
           fetch-depth: 0
 
+      - name: Clean up Disk space
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
       - name: Run nf-test
         id: run_nf_test
         uses: ./.github/actions/nf-test

From 40692ac03ce15458f3a4128e9f7a98f5e7a62f1c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 17 Jul 2025 14:49:16 +0200
Subject: [PATCH 655/887] add paths to s3 refs

---
 conf/genomes.config                           |  33 ++
 ..._ignored.config => genomes_ignored.config} |   0
 conf/igenomes.config                          | 478 ------------------
 conf/test_full.config                         |   5 +-
 main.nf                                       |  26 +-
 nextflow.config                               |  24 +-
 6 files changed, 65 insertions(+), 501 deletions(-)
 create mode 100644 conf/genomes.config
 rename conf/{igenomes_ignored.config => genomes_ignored.config} (100%)
 delete mode 100644 conf/igenomes.config

diff --git a/conf/genomes.config b/conf/genomes.config
new file mode 100644
index 000000000..960ee80f8
--- /dev/null
+++ b/conf/genomes.config
@@ -0,0 +1,33 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Nextflow config file for Rnafusion reference paths
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Defines reference genomes using paths.
+    Can be used by any config that customises the base path using:
+        $params.genomes_base / --genomes_base
+----------------------------------------------------------------------------------------
+*/
+
+params {
+    // rnafusion reference file paths
+    genomes {
+        'GRCh38' {
+            fasta                       = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode/Homo_sapiens_GRCh38_${params.genome_gencode_version}_dna_primary_assembly.fa"
+            fai                         = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode/Homo_sapiens_GRCh38_${params.genome_gencode_version}_dna_primary_assembly.fa.fai"
+            gtf                         = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode/Homo_sapiens_GRCh38_${params.genome_gencode_version}.gtf"
+            refflat                     = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode/Homo_sapiens_GRCh38_${params.genome_gencode_version}.gtf.refflat"
+            rrna_intervals              = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode/Homo_sapiens_GRCh38_${params.genome_gencode_version}.gtf.interval_list"
+            arriba_ref_blacklist        = "${params.genomes_base}/GRCh38/arriba/2.5.0/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+            arriba_ref_cytobands        = "${params.genomes_base}/GRCh38/arriba/2.5.0/cytobands_hg38_GRCh38_v2.5.0.tsv"
+            arriba_ref_known_fusions    = "${params.genomes_base}/GRCh38/arriba/2.5.0/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+            arriba_ref_protein_domains  = "${params.genomes_base}/GRCh38/arriba/2.5.0/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+            fusioncatcher_ref           = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}fusioncatcher/"
+            hgnc_ref                    = "${params.genomes_base}/GRCh38/hgnc/hgnc_complete_set.txt"
+            hgnc_date                   = "${params.genomes_base}/GRCh38/hgnc/HGNC-DB-timestamp.txt"
+            salmon_index                = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/salmon"
+            starfusion_ref              = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/starfusion/ctat_genome_lib_build_dir"
+            starindex_ref               = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/star"
+            fusionreport_ref            = "${params.genomes_base}/GRCh38/fusion_report_db"
+        }
+    }
+}
diff --git a/conf/igenomes_ignored.config b/conf/genomes_ignored.config
similarity index 100%
rename from conf/igenomes_ignored.config
rename to conf/genomes_ignored.config
diff --git a/conf/igenomes.config b/conf/igenomes.config
deleted file mode 100644
index d608b45bf..000000000
--- a/conf/igenomes.config
+++ /dev/null
@@ -1,478 +0,0 @@
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Nextflow config file for iGenomes paths
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Defines reference genomes using iGenome paths.
-    Can be used by any config that customises the base path using:
-        $params.igenomes_base / --igenomes_base
-----------------------------------------------------------------------------------------
-*/
-
-params {
-    // illumina iGenomes reference file paths
-    genomes {
-        'GRCh37' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed"
-            mirtrace_species = "hsa"
-        }
-        'GRCh38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-            mirtrace_species = "hsa"
-        }
-        'CHM13' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/"
-            bwamem2     = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf"
-            gff         = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz"
-            mito_name   = "chrM"
-        }
-        'GRCm38' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed"
-            mirtrace_species = "mmu"
-        }
-        'TAIR10' {
-            fasta       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt"
-            mito_name   = "Mt"
-            mirtrace_species = "ath"
-        }
-        'EB2' {
-            fasta       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt"
-            // mirtrace_species = "bsu"
-        }
-        'UMD3.1' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt"
-            mito_name   = "MT"
-            mirtrace_species = "bta"
-        }
-        'WBcel235' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed"
-            mito_name   = "MtDNA"
-            macs_gsize  = "9e7"
-            mirtrace_species = "cel"
-        }
-        'CanFam3.1' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt"
-            mito_name   = "MT"
-            mirtrace_species = "cfa"
-        }
-        'GRCz10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            mirtrace_species = "dre"
-        }
-        'BDGP6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed"
-            mito_name   = "M"
-            macs_gsize  = "1.2e8"
-            mirtrace_species = "dme"
-        }
-        'EquCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt"
-            mito_name   = "MT"
-            // mirtrace_species = "ecb"
-        }
-        'EB1' {
-            fasta       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt"
-            // mirtrace_species = "ecd"
-        }
-        'Galgal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            mirtrace_species = "gga"
-        }
-        'Gm01' {
-            fasta       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt"
-            // mirtrace_species = "gmx"
-        }
-        'Mmul_1' {
-            fasta       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt"
-            mito_name   = "MT"
-            // mirtrace_species = "mcc"
-        }
-        'IRGSP-1.0' {
-            fasta       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-            mirtrace_species = "osa"
-        }
-        'CHIMP2.1.4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt"
-            mito_name   = "MT"
-            mirtrace_species = "ptr"
-        }
-        'Rnor_5.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            mirtrace_species = "rno"
-        }
-        'Rnor_6.0' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            mirtrace_species = "rno"
-        }
-        'R64-1-1' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed"
-            mito_name   = "MT"
-            macs_gsize  = "1.2e7"
-            // mirtrace_species = "sce"
-        }
-        'EF2' {
-            fasta       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt"
-            mito_name   = "MT"
-            macs_gsize  = "1.21e7"
-            // mirtrace_species = "spo"
-        }
-        'Sbi1' {
-            fasta       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt"
-            mirtrace_species = "sbi"
-        }
-        'Sscrofa10.2' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt"
-            mito_name   = "MT"
-            mirtrace_species = "ssc"
-        }
-        'AGPv3' {
-            fasta       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed"
-            mito_name   = "Mt"
-            mirtrace_species = "zma"
-        }
-        'hg38' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg38-blacklist.bed"
-            mirtrace_species = "hsa"
-        }
-        'hg19' {
-            fasta       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "2.7e9"
-            blacklist   = "${projectDir}/assets/blacklists/hg19-blacklist.bed"
-            mirtrace_species = "hsa"
-        }
-        'mm10' {
-            fasta       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.87e9"
-            blacklist   = "${projectDir}/assets/blacklists/mm10-blacklist.bed"
-            mirtrace_species = "mmu"
-        }
-        'bosTau8' {
-            fasta       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            mirtrace_species = "bta"
-        }
-        'ce10' {
-            fasta       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "9e7"
-            mirtrace_species = "cel"
-        }
-        'canFam3' {
-            fasta       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt"
-            mito_name   = "chrM"
-            mirtrace_species = "cfa"
-        }
-        'danRer10' {
-            fasta       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.37e9"
-            mirtrace_species = "dre"
-        }
-        'dm6' {
-            fasta       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e8"
-            mirtrace_species = "dme"
-        }
-        'equCab2' {
-            fasta       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt"
-            mito_name   = "chrM"
-            // mirtrace_species = "ecb"
-        }
-        'galGal4' {
-            fasta       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt"
-            mito_name   = "chrM"
-            mirtrace_species = "gga"
-        }
-        'panTro4' {
-            fasta       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt"
-            mito_name   = "chrM"
-            mirtrace_species = "ptr"
-        }
-        'rn6' {
-            fasta       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed"
-            mito_name   = "chrM"
-            mirtrace_species = "rno"
-        }
-        'sacCer3' {
-            fasta       = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
-            readme      = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt"
-            mito_name   = "chrM"
-            macs_gsize  = "1.2e7"
-            // mirtrace_species = "sce"
-        }
-        'susScr3' {
-            fasta       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
-            bwa         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/"
-            bowtie2     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
-            star        = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
-            bismark     = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
-            gtf         = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf"
-            bed12       = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed"
-            readme      = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt"
-            mito_name   = "chrM"
-            mirtrace_species = "ssc"
-        }
-    }
-}
diff --git a/conf/test_full.config b/conf/test_full.config
index cfcb7865b..cd1992fea 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -14,9 +14,6 @@ params {
 
     // Input data for full size test
     input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-    all   = true
-    // TODO
-    // test_full can't run currently because the references must be given and they are not available.
-    // This profile should be updated once they get uploaded.
+    tools = "all"
 
     }
diff --git a/main.nf b/main.nf
index 3161d45a2..2408f9c2c 100644
--- a/main.nf
+++ b/main.nf
@@ -9,6 +9,31 @@
 ----------------------------------------------------------------------------------------
 */
 
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    GENOME PARAMETER VALUES
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+*/
+
+include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
+
+params.fasta                        = getGenomeAttribute("fasta")
+params.fai                          = getGenomeAttribute("fai")
+params.gtf                          = getGenomeAttribute("gtf")
+params.refflat                      = getGenomeAttribute("refflat")
+params.rrna_intervals               = getGenomeAttribute("rrna_intervals")
+params.arriba_ref_blacklist         = getGenomeAttribute("arriba_ref_blacklist")
+params.arriba_ref_cytobands         = getGenomeAttribute("arriba_ref_cytobands")
+params.arriba_ref_known_fusions     = getGenomeAttribute("arriba_ref_known_fusions")
+params.arriba_ref_protein_domains   = getGenomeAttribute("arriba_ref_protein_domains")
+params.fusioncatcher_ref            = getGenomeAttribute("fusioncatcher_ref")
+params.hgnc_ref                     = getGenomeAttribute("hgnc_ref")
+params.hgnc_date                    = getGenomeAttribute("hgnc_date")
+params.salmon_index                 = getGenomeAttribute("salmon_index")
+params.starfusion_ref               = getGenomeAttribute("starfusion_ref")
+params.starindex_ref                = getGenomeAttribute("starindex_ref")
+params.fusionreport_ref             = getGenomeAttribute("fusionreport_ref")
+
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
     IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS
@@ -17,7 +42,6 @@
 
 include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { PIPELINE_COMPLETION     } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
-include { getGenomeAttribute      } from './subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { RNAFUSION               } from './workflows/rnafusion'
 
 /*
diff --git a/nextflow.config b/nextflow.config
index d46ef7e22..023232c3a 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -26,7 +26,8 @@ params {
 
     // Genome
     genome                     = 'GRCh38'
-    genomes_base               = "${params.outdir}/references"
+    genomes_base               = "s3://nf-core-awsmegatests/rnafusion/references"
+    genomes_ignore             = false
     genome_gencode_version     = 46
     read_length                = 100
     genomes                    = [:]
@@ -65,24 +66,8 @@ params {
     download_refs               = false
     fusioncatcher_download_link = "https://master.dl.sourceforge.net/project/rnafusion/fusioncatcher"
 
-    // Path to references
-    fasta                         = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa"
-    fai                           = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}_dna_primary_assembly.fa.fai"
-    gtf                           = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf"
-    refflat                       = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.gtf.refflat"
-    rrna_intervals                = "${params.genomes_base}/gencode/Homo_sapiens_${params.genome}_${params.genome_gencode_version}.interval_list"
+    // Reference options
     no_cosmic                     = false
-    arriba_ref_blacklist          = "${params.genomes_base}/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz"
-    arriba_ref_cytobands          = "${params.genomes_base}/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv"
-    arriba_ref_known_fusions      = "${params.genomes_base}/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz"
-    arriba_ref_protein_domains    = "${params.genomes_base}/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3"
-    fusioncatcher_ref             = "${params.genomes_base}/fusioncatcher/human_v${params.genome_gencode_version}"
-    hgnc_ref                      = "${params.genomes_base}/hgnc/hgnc_complete_set.txt"
-    hgnc_date                     = "${params.genomes_base}/hgnc/HGNC-DB-timestamp.txt"
-    salmon_index                  = "${params.genomes_base}/salmon/salmon"
-    starfusion_ref                = "${params.genomes_base}/starfusion/ctat_genome_lib_build_dir"
-    starindex_ref                 = "${params.genomes_base}/star"
-    fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
     ctatsplicing_cancer_introns   = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
 
     // Path to fusion outputs
@@ -256,6 +241,9 @@ podman.registry       = 'quay.io'
 singularity.registry  = 'quay.io'
 charliecloud.registry = 'quay.io'
 
+// Load genomes.config if required
+includeConfig !params.genomes_ignore ? 'conf/genomes.config' : 'conf/genomes_ignored.config'
+
 // Export these variables to prevent local Python/R libraries from conflicting with those in the container
 // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container.
 // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable.

From 557c29f6a129a31a80a016415e648647864fd0b3 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 17 Jul 2025 15:14:29 +0200
Subject: [PATCH 656/887] add genomes_ignore to schema

---
 nextflow_schema.json | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index a0794cd62..7262b727e 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -76,6 +76,11 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to reference folder"
                 },
+                "genomes_ignore": {
+                    "type": "string",
+                    "fa_icon": "far fa-file-code",
+                    "description": "Don't automatically assign reference parameters to the correct references in --genomes_base"
+                },
                 "genome_gencode_version": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",

From 79b3957168c05d4957db2f233989414a4e31e1cf Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 17 Jul 2025 15:23:37 +0200
Subject: [PATCH 657/887] allow gzipped TSV files for arriba

---
 nextflow_schema.json | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 7262b727e..f55e974b9 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -102,7 +102,7 @@
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "format": "file-path",
-                    "pattern": "\\S+\\.tsv$",
+                    "pattern": "\\S+\\.tsv(\\.gz)?$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
                 },
@@ -116,7 +116,7 @@
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "format": "file-path",
-                    "pattern": "\\S+\\.tsv$",
+                    "pattern": "\\S+\\.tsv(\\.gz)?$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference known fusions"
                 },

From e66edd5763b521be58477e4caefed210566c9bcf Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 17 Jul 2025 15:29:38 +0200
Subject: [PATCH 658/887] fix typo in fusioncatcher ref

---
 conf/genomes.config | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/conf/genomes.config b/conf/genomes.config
index 960ee80f8..7a2a0ad99 100644
--- a/conf/genomes.config
+++ b/conf/genomes.config
@@ -21,7 +21,7 @@ params {
             arriba_ref_cytobands        = "${params.genomes_base}/GRCh38/arriba/2.5.0/cytobands_hg38_GRCh38_v2.5.0.tsv"
             arriba_ref_known_fusions    = "${params.genomes_base}/GRCh38/arriba/2.5.0/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
             arriba_ref_protein_domains  = "${params.genomes_base}/GRCh38/arriba/2.5.0/protein_domains_hg38_GRCh38_v2.5.0.gff3"
-            fusioncatcher_ref           = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}fusioncatcher/"
+            fusioncatcher_ref           = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/fusioncatcher/"
             hgnc_ref                    = "${params.genomes_base}/GRCh38/hgnc/hgnc_complete_set.txt"
             hgnc_date                   = "${params.genomes_base}/GRCh38/hgnc/HGNC-DB-timestamp.txt"
             salmon_index                = "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/salmon"

From 7f53a11abae6b171721396567060260622c8287c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 17 Jul 2025 16:01:44 +0200
Subject: [PATCH 659/887] remove download modules and check for existence of
 those files

---
 modules.json                                  | 131 +++++++++++++-----
 .../fusioncatcher/download/environment.yml    |   5 -
 modules/local/fusioncatcher/download/main.nf  |  46 ------
 modules/local/fusioncatcher/download/meta.yml |  25 ----
 .../nf-core/arriba/download/environment.yml   |   8 --
 modules/nf-core/arriba/download/main.nf       |  51 -------
 modules/nf-core/arriba/download/meta.yml      |  52 -------
 .../arriba/download/tests/main.nf.test        |  50 -------
 .../arriba/download/tests/main.nf.test.snap   |  43 ------
 nextflow_schema.json                          |   5 +
 subworkflows/local/build_references/main.nf   |  35 +----
 11 files changed, 104 insertions(+), 347 deletions(-)
 delete mode 100644 modules/local/fusioncatcher/download/environment.yml
 delete mode 100644 modules/local/fusioncatcher/download/main.nf
 delete mode 100644 modules/local/fusioncatcher/download/meta.yml
 delete mode 100644 modules/nf-core/arriba/download/environment.yml
 delete mode 100644 modules/nf-core/arriba/download/main.nf
 delete mode 100644 modules/nf-core/arriba/download/meta.yml
 delete mode 100644 modules/nf-core/arriba/download/tests/main.nf.test
 delete mode 100644 modules/nf-core/arriba/download/tests/main.nf.test.snap

diff --git a/modules.json b/modules.json
index d8c39f79e..b7a33c03f 100644
--- a/modules.json
+++ b/modules.json
@@ -8,147 +8,198 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
-                        "installed_by": ["modules"]
-                    },
-                    "arriba/download": {
-                        "branch": "master",
-                        "git_sha": "98bd7be6d2191b0d5900ee698d91b52eeab90311",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "1037205706929921360f57e8042eae6819825e31",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fusionreport/detect": {
                         "branch": "master",
                         "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fusionreport/download": {
                         "branch": "master",
                         "git_sha": "df4b662dae05afb695a4326fc172178b5a7a48ce",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "1ec937ab3edc307bc0d79a2200d784e9f0868359",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "7b50cb7be890e4b28cffb82e438cc6a8d7805d3f",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "980a4964be91da75fb3ccf576118a18e247f6d15",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/convert": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     }
                 }
             },
@@ -157,20 +208,26 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/local/fusioncatcher/download/environment.yml b/modules/local/fusioncatcher/download/environment.yml
deleted file mode 100644
index 8e83de36b..000000000
--- a/modules/local/fusioncatcher/download/environment.yml
+++ /dev/null
@@ -1,5 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::fusioncatcher=1.33
diff --git a/modules/local/fusioncatcher/download/main.nf b/modules/local/fusioncatcher/download/main.nf
deleted file mode 100644
index 2cc34f052..000000000
--- a/modules/local/fusioncatcher/download/main.nf
+++ /dev/null
@@ -1,46 +0,0 @@
-process FUSIONCATCHER_DOWNLOAD {
-    tag "fusioncatcher_download"
-    label 'process_medium'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3b/3b54fa9135194c72a18d00db6b399c03248103f87e43ca75e4b50d61179994b3/data':
-        'community.wave.seqera.io/library/wget:1.21.4--8b0fcde81c17be5e' }"
-
-    input:
-    val genome_gencode_version
-
-    output:
-    tuple env(meta), path("human_v*"), emit: reference
-    path "versions.yml"              , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-
-    def args = task.ext.args ?: ''
-    meta = [ id: "human_v${genome_gencode_version}" ]
-    """
-    wget --no-check-certificate $args ${params.fusioncatcher_download_link}
-    tar xz human_v${genome_gencode_version}.tar.gz
-    rm human_v${genome_gencode_version}.tar*
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
-        tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    mkdir human_v${genome_gencode_version}
-    touch human_v${genome_gencode_version}/ensembl_fully_overlapping_genes.txt
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        wget: \$(wget --version | head -1 | cut -d ' ' -f 3)
-        tar: \$(tar --version | head -1 | sed -e 's/tar (GNU tar) //')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/fusioncatcher/download/meta.yml b/modules/local/fusioncatcher/download/meta.yml
deleted file mode 100644
index 40421a4ee..000000000
--- a/modules/local/fusioncatcher/download/meta.yml
+++ /dev/null
@@ -1,25 +0,0 @@
-name: fusioncatcher_download
-description: Build genome for fusioncatcher
-keywords:
-  - sort
-tools:
-  - fusioncatcher:
-      description: Build genome for fusioncatcher
-      homepage: https://github.com/ndaniel/fusioncatcher/
-      documentation: https://github.com/ndaniel/fusioncatcher/blob/master/doc/manual.md
-      tool_dev_url: https://github.com/ndaniel/fusioncatcher/
-      doi: "10.1101/011650"
-      licence: ["GPL v3"]
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - reference:
-      type: directory
-      description: Path to fusioncatcher references
-      pattern: "*"
-
-authors:
-  - "@praveenraj2018, @rannick"
diff --git a/modules/nf-core/arriba/download/environment.yml b/modules/nf-core/arriba/download/environment.yml
deleted file mode 100644
index b30731312..000000000
--- a/modules/nf-core/arriba/download/environment.yml
+++ /dev/null
@@ -1,8 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::arriba=2.5.0
-  - conda-forge::wget=1.21.4
diff --git a/modules/nf-core/arriba/download/main.nf b/modules/nf-core/arriba/download/main.nf
deleted file mode 100644
index baaf72f24..000000000
--- a/modules/nf-core/arriba/download/main.nf
+++ /dev/null
@@ -1,51 +0,0 @@
-process ARRIBA_DOWNLOAD {
-    tag "arriba"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/27/27475cdcdbcc8c0ffb6b5ca8c2e6567dbe490edb96f5df4e8f01f4f95912dcd3/data' :
-        'community.wave.seqera.io/library/arriba_wget:a3e48cf793a0b654' }"
-
-    input:
-    val(genome)
-
-    output:
-    path "blacklist*${genome}*.tsv.gz"    , emit: blacklist
-    path "cytobands*${genome}*.tsv"       , emit: cytobands
-    path "protein_domains*${genome}*.gff3", emit: protein_domains
-    path "known_fusions*${genome}*.tsv.gz", emit: known_fusions
-    path "versions.yml"                   , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def arriba_version = '2.5.0'
-    """
-    wget https://github.com/suhrig/arriba/releases/download/v${arriba_version}/arriba_v${arriba_version}.tar.gz -O arriba_v${arriba_version}.tar.gz --no-check-certificate
-    tar -xzvf arriba_v${arriba_version}.tar.gz
-    rm arriba_v${arriba_version}.tar.gz
-    mv arriba_v${arriba_version}/database/* .
-    rm -r arriba_v${arriba_version}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        arriba_download: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
-    END_VERSIONS
-    """
-
-    stub:
-    def arriba_version = '2.5.0'
-    """
-    echo | gzip > blacklist_hg38_GRCh38_v${arriba_version}.tsv.gz
-    touch protein_domains_hg38_GRCh38_v${arriba_version}.gff3
-    touch cytobands_hg38_GRCh38_v${arriba_version}.tsv
-    echo | gzip > known_fusions_hg38_GRCh38_v${arriba_version}.tsv.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        arriba_download: \$(arriba -h | grep 'Version:' 2>&1 |  sed 's/Version:\s//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/arriba/download/meta.yml b/modules/nf-core/arriba/download/meta.yml
deleted file mode 100644
index abeb9f8fd..000000000
--- a/modules/nf-core/arriba/download/meta.yml
+++ /dev/null
@@ -1,52 +0,0 @@
-name: arriba_download
-description: Arriba is a command-line tool for the detection of gene fusions from
-  RNA-Seq data.
-keywords:
-  - fusion
-  - arriba
-  - reference
-tools:
-  - arriba:
-      description: Fast and accurate gene fusion detection from RNA-Seq data
-      homepage: https://github.com/suhrig/arriba
-      documentation: https://arriba.readthedocs.io/en/latest/
-      tool_dev_url: https://github.com/suhrig/arriba
-      doi: "10.1101/gr.257246.119"
-      licence: ["MIT"]
-      identifier: biotools:Arriba
-
-input:
-  - - genome:
-        type: string
-        description: hg38, hg19, GRCh38, GRCh37 for humans are accepted
-output:
-  - blacklist:
-      - blacklist*${genome}*.tsv.gz:
-          type: string
-          description: The blacklist removes recurrent alignment artifacts and transcripts
-            which are present in healthy tissue
-          pattern: ".tsv"
-  - cytobands:
-      - cytobands*${genome}*.tsv:
-          type: file
-          description: Coordinates of the Giemsa staining bands. This information is used
-            to draw ideograms
-          pattern: ".tsv"
-  - protein_domains:
-      - protein_domains*${genome}*.gff3:
-          type: file
-          description: Protein domain annotations
-          pattern: "*.gff3"
-  - known_fusions:
-      - known_fusions*${genome}*.tsv.gz:
-          type: file
-          description: Arriba is more sensitive to those fusions to improve the detection
-            rate of expected or highly relevant events, such as recurrent fusions
-          pattern: "*.tsv"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-authors:
-  - "@peterpru"
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test b/modules/nf-core/arriba/download/tests/main.nf.test
deleted file mode 100644
index 2c32c7a0f..000000000
--- a/modules/nf-core/arriba/download/tests/main.nf.test
+++ /dev/null
@@ -1,50 +0,0 @@
-
-nextflow_process {
-
-    name "Test Process ARRIBA_DOWNLOAD"
-    script "../main.nf"
-    process "ARRIBA_DOWNLOAD"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "arriba"
-    tag "arriba/download"
-
-    test("test-arriba-download") {
-        when {
-            process {
-                """
-                input[0] = 'GRCh38'
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("download - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = 'GRCh38'
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success }
-            )
-        }
-    }
-
-}
diff --git a/modules/nf-core/arriba/download/tests/main.nf.test.snap b/modules/nf-core/arriba/download/tests/main.nf.test.snap
deleted file mode 100644
index ec25745a7..000000000
--- a/modules/nf-core/arriba/download/tests/main.nf.test.snap
+++ /dev/null
@@ -1,43 +0,0 @@
-{
-    "test-arriba-download": {
-        "content": [
-            {
-                "0": [
-                    "blacklist_hg38_GRCh38_v2.5.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
-                ],
-                "1": [
-                    "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
-                ],
-                "2": [
-                    "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
-                ],
-                "3": [
-                    "known_fusions_hg38_GRCh38_v2.5.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
-                ],
-                "4": [
-                    "versions.yml:md5,2457cdc32bc582854616aba806a91d35"
-                ],
-                "blacklist": [
-                    "blacklist_hg38_GRCh38_v2.5.0.tsv.gz:md5,e3098a4be51aece78aede64b55c39318"
-                ],
-                "cytobands": [
-                    "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c"
-                ],
-                "known_fusions": [
-                    "known_fusions_hg38_GRCh38_v2.5.0.tsv.gz:md5,4f00f81ccb5f4db283f1a22b8b0da67c"
-                ],
-                "protein_domains": [
-                    "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978"
-                ],
-                "versions": [
-                    "versions.yml:md5,2457cdc32bc582854616aba806a91d35"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-14T11:54:10.203072"
-    }
-}
\ No newline at end of file
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f55e974b9..ba3a60d88 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -102,6 +102,7 @@
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "format": "file-path",
+                    "exists": true,
                     "pattern": "\\S+\\.tsv(\\.gz)?$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
@@ -109,6 +110,7 @@
                 "arriba_ref_cytobands": {
                     "type": "string",
                     "format": "file-path",
+                    "exists": true,
                     "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference cytobands"
@@ -116,6 +118,7 @@
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "format": "file-path",
+                    "exists": true,
                     "pattern": "\\S+\\.tsv(\\.gz)?$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference known fusions"
@@ -123,6 +126,7 @@
                 "arriba_ref_protein_domains": {
                     "type": "string",
                     "format": "file-path",
+                    "exists": true,
                     "pattern": "\\S+\\.gff3$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference protein domain"
@@ -158,6 +162,7 @@
                 "fusioncatcher_ref": {
                     "type": "string",
                     "format": "directory-path",
+                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 5751627ff..3ee8e9a2b 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -133,37 +133,12 @@ workflow BUILD_REFERENCES {
         }
     }
 
-    def ch_arriba_ref_blacklist       = Channel.empty()
-    def ch_arriba_ref_cytobands       = Channel.empty()
-    def ch_arriba_ref_known_fusions   = Channel.empty()
-    def ch_arriba_ref_protein_domains = Channel.empty()
-    if (tools.contains("arriba")) {
-        if (!exists_not_empty(params.arriba_ref_blacklist) || !exists_not_empty(params.arriba_ref_known_fusions) || !exists_not_empty(params.arriba_ref_protein_domains) || !exists_not_empty(params.arriba_ref_cytobands)) {
-            ARRIBA_DOWNLOAD(params.genome)
-            ch_versions = ch_versions.mix(ARRIBA_DOWNLOAD.out.versions)
-            ch_arriba_ref_blacklist       = ARRIBA_DOWNLOAD.out.blacklist
-            ch_arriba_ref_cytobands       = ARRIBA_DOWNLOAD.out.cytobands
-            ch_arriba_ref_known_fusions   = ARRIBA_DOWNLOAD.out.known_fusions
-            ch_arriba_ref_protein_domains = ARRIBA_DOWNLOAD.out.protein_domains
-        } else {
-            ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist)
-            ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands)
-            ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions)
-            ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
-        }
-    }
+    def ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist)
+    def ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands)
+    def ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions)
+    def ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
 
-    def ch_fusioncatcher_ref = Channel.empty()
-    if (tools.contains("fusioncatcher")) {
-        if (!exists_not_empty(params.fusioncatcher_ref)) {
-            FUSIONCATCHER_DOWNLOAD(params.genome_gencode_version)
-            ch_versions = ch_versions.mix(FUSIONCATCHER_DOWNLOAD.out.versions)
-            ch_fusioncatcher_ref = FUSIONCATCHER_DOWNLOAD.out.reference
-        }
-        else {
-            ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { it -> [[id:it.name], it] }
-        }
-    }
+    def ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { it -> [[id:it.name], it] }
 
     def ch_starfusion_ref = Channel.empty()
     if (tools.intersect(["starfusion", "ctatsplicing", "fusioninspector"])) {

From b3feb46bbbaf4388e2195f6f35f3d55235dfeed4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 16:22:35 +0200
Subject: [PATCH 660/887] update metro map

---
 docs/images/nf-core-rnafusion_metro_map.png | Bin 93105 -> 165546 bytes
 docs/images/nf-core-rnafusion_metro_map.svg | 721 +++++++++-----------
 2 files changed, 315 insertions(+), 406 deletions(-)

diff --git a/docs/images/nf-core-rnafusion_metro_map.png b/docs/images/nf-core-rnafusion_metro_map.png
index 76b6bc3fdb340e22d794e31b7d1d98aec4d44151..d48e484b9e6ce74eb4528689723cf8c2bcf87b2f 100644
GIT binary patch
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-         y="142.20734">Workflows:</tspan></text>
-    <g
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-      <path
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-           x="41.872887"
-           y="139.50002">arriba</tspan></text>
-      <text
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        xml:space="preserve"
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        x="281.53165"
-       y="61.410133"
+       y="85.222656"
        id="text599"><tspan
          sodipodi:role="line"
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          x="281.53165"
-         y="61.410133">Arriba</tspan><tspan
+         y="85.222656">Arriba</tspan><tspan
          sodipodi:role="line"
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          x="281.53165"
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+         y="90.51432"
          id="tspan597">visualisation</tspan></text>
     <text
        xml:space="preserve"
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        x="281.34973"
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+       y="116.17358"
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          x="281.34973"
-         y="92.361061">VCF</tspan><tspan
+         y="116.17358">VCF</tspan><tspan
          sodipodi:role="line"
          style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro Bold';text-align:center;text-anchor:middle;stroke-width:0.264583"
          x="281.34973"
-         y="97.652725"
+         y="121.46525"
          id="tspan597-6">collect</tspan></text>
     <circle
-       style="fill:#ffffff;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill;stop-color:#000000"
+       style="fill:#000000;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill;stop-color:#000000;fill-opacity:1"
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        cx="264.96671"
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+       cy="113.05365"
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     <ellipse
-       style="fill:#ffffff;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill;stop-color:#000000"
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        cx="239.70604"
-       cy="80.030518"
+       cy="103.84303"
        rx="2.6459999"
        ry="2.6458333" />
+    <text
+       xml:space="preserve"
+       style="font-size:4.23333px;line-height:1.25;font-family:Commissioner;-inkscape-font-specification:Commissioner;text-align:center;letter-spacing:0px;text-anchor:middle;stroke-width:0.264583"
+       x="55.225376"
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+         x="55.225376"
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+         x="55.225376"
+         y="92.71003"
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+         sodipodi:role="line"
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+         x="55.225376"
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+    <circle
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+       id="path3447-4-3-5"
+       cx="43.456852"
+       cy="80.625168"
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+    <circle
+       style="fill:#000000;fill-opacity:1;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill;stop-color:#000000"
+       id="path3447-4-3-5-6"
+       cx="11.261717"
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+    <text
+       xml:space="preserve"
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+       x="26.674341"
+       y="74.297089"
+       id="text20792-6-8-5-1"><tspan
+         sodipodi:role="line"
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+         x="26.674341"
+         y="74.297089">fastqc</tspan><tspan
+         sodipodi:role="line"
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+         x="26.674341"
+         y="79.588753"
+         id="tspan6319-3-4" /></text>
+    <circle
+       style="fill:#000000;fill-opacity:1;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill;stop-color:#000000"
+       id="path3447-4-3-6-9"
+       cx="27.359283"
+       cy="80.625168"
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+    <text
+       xml:space="preserve"
+       style="font-size:4.23333px;line-height:1.25;font-family:Commissioner;-inkscape-font-specification:Commissioner;letter-spacing:0px;stroke-width:0.264583"
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+         x="97.201881"
+         y="118.62772">FusionCatcher</tspan></text>
+    <path
+       style="fill:none;stroke:#0050ef;stroke-width:2.65201;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
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+       id="path1232-7-2-7-3-0-5-0"
+       sodipodi:nodetypes="cssc" />
+    <path
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+       sodipodi:nodetypes="cc" />
+    <circle
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+       id="path3447-7-4-3-1-1-5"
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+       id="path3447-4-6"
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   </g>
 </svg>

From f34f4b5559124a41501e6b7285de787749d282b4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 16:37:22 +0200
Subject: [PATCH 661/887] update doc

---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index 6a8530c18..607a2d43d 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -16,7 +16,7 @@ The pipeline is divided into two parts:
 
 2. Detecting fusions
 
-- Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, `StringTie` and `CTAT-SPLICING`
+- Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, `StringTie` and `CTAT-SPLICING` (the last two are not supported by fusion-report and `StringTie` can be seen as more of an assembly tool, requires manual investigation).
 - QC: `Fastqc`, `MultiQC`, and `Picard CollectInsertSize`, `Picard CollectWgsMetrics`, `Picard Markduplicates`
 - Fusions visualization: `Arriba`, `fusion-report`, `FusionInspector`, and `vcf_collect`
 

From 74d947b77f210b804aeb358cc58a93fa13d62003 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 17 Jul 2025 16:38:16 +0200
Subject: [PATCH 662/887] changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4383082d3..bc1a28161 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -85,6 +85,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Replaced local `FUSIONREPORT` and `FUSIONREPORT_DOWNLOAD` with `FUSIONREPORT_DETECT` and `FUSIONREPORT_DOWNLOAD` from nf-core [#703](https://github.com/nf-core/rnafusion/pull/703)
 - Updated arriba to v2.5.0 [#693](https://github.com/nf-core/rnafusion/pull/693)
 - Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
+- Slight documentation update [#719](https://github.com/nf-core/rnafusion/pull/719)
 
 ### Fixed
 

From 94ccaaafdce542e3ed98e15aa8147a406c01b113 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Thu, 17 Jul 2025 16:29:38 +0000
Subject: [PATCH 663/887] Remove stub in test

---
 tests/test_build.nf.test | 3 +--
 1 file changed, 1 insertion(+), 2 deletions(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index f960df0f4..61e5b1e9f 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -3,7 +3,6 @@ nextflow_pipeline {
     name "Test pipeline"
     script "../main.nf"
     profile "test_build"
-    options "-stub"
     tag "pipeline"
     tag "pipeline_rnafusion"
     tag "test_build"
@@ -24,7 +23,7 @@ nextflow_pipeline {
                 gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
                 fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 species                    = 'homo_sapiens'
-                dfam_version               = '3.4'
+                dfam_version               = 3.4
                 outdir                     = "$outputDir"
             }
         }

From 3351daf4dba7d5ca76814eda9652f18ff6bf7f5b Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 17 Jul 2025 16:53:01 +0000
Subject: [PATCH 664/887] feat: add codeowners file

---
 .github/CODEOWNERS | 12 ++++++++++++
 1 file changed, 12 insertions(+)
 create mode 100644 .github/CODEOWNERS

diff --git a/.github/CODEOWNERS b/.github/CODEOWNERS
new file mode 100644
index 000000000..0a8861b61
--- /dev/null
+++ b/.github/CODEOWNERS
@@ -0,0 +1,12 @@
+# Maintainers
+* @rannick @nvnieuwk @atrigila
+*.nf.test*  @rannick @nvnieuwk @atrigila
+.github/workflows/  @rannick @nvnieuwk @atrigila
+
+# Subworkflows
+subworkflows/nf-core/*  @rannick @nvnieuwk @atrigila
+subworkflows/local/*  @rannick @nvnieuwk @atrigila
+
+# Modules
+modules/nf-core/*  @rannick @nvnieuwk @atrigila
+modules/local/*  @rannick @nvnieuwk @atrigila

From b6332aaf159bb9c8129854d61e96304454855f5b Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 17 Jul 2025 17:26:26 +0000
Subject: [PATCH 665/887] fix: fix failing ci test

---
 tests/test_build.nf.test | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 61e5b1e9f..0f5387d03 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -33,7 +33,7 @@ nextflow_pipeline {
             // Ignore files with timestamps in their names
             def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
             // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore', 'PFAM.domtblout.dat.gz', 'blast_pairs.*', '')
+            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
             assertAll(
                 { assert workflow.success},
                 { assert snapshot(

From 49f41907e9533ab33446f139d25a4ed1d41e882d Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Thu, 17 Jul 2025 17:59:51 +0000
Subject: [PATCH 666/887] Modify schema.json to allow for more flexibility in
 input or dfam urls

---
 nextflow_schema.json                                 | 12 ++++++------
 .../local/utils_nfcore_rnafusion_pipeline/main.nf    |  4 +---
 2 files changed, 7 insertions(+), 9 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index 2d304bd0a..deee57b44 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -233,7 +233,7 @@
                 "dfam_hmm": {
                     "type": "string",
                     "format": "uri",
-                    "pattern": "^https?://.*",
+                    "pattern": "\\S+\\.hmm$",
                     "description": "URL to Dfam HMM database file",
                     "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam HMM database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
@@ -241,7 +241,7 @@
                 "dfam_h3f": {
                     "type": "string",
                     "format": "uri",
-                    "pattern": "^https?://.*",
+                    "pattern": "\\S+\\.h3f$",
                     "description": "URL to Dfam H3F database file",
                     "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3F database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
@@ -249,7 +249,7 @@
                 "dfam_h3i": {
                     "type": "string",
                     "format": "uri",
-                    "pattern": "^https?://.*",
+                    "pattern": "\\S+\\.h3i$",
                     "description": "URL to Dfam H3I database file",
                     "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3I database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
@@ -257,7 +257,7 @@
                 "dfam_h3m": {
                     "type": "string",
                     "format": "uri",
-                    "pattern": "^https?://.*",
+                    "pattern": "\\S+\\.h3m$",
                     "description": "URL to Dfam H3M database file",
                     "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3M database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
@@ -265,7 +265,7 @@
                 "dfam_h3p": {
                     "type": "string",
                     "format": "uri",
-                    "pattern": "^https?://.*",
+                    "pattern": "\\S+\\.h3p$",
                     "description": "URL to Dfam H3P database file",
                     "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3P database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
@@ -273,7 +273,7 @@
                 "pfam_url": {
                     "type": "string",
                     "format": "uri",
-                    "pattern": "^https?://.*",
+                    "pattern": "\\S+\\.hmm(\\.gz)$",
                     "description": "URL to Pfam database file",
                     "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Pfam database file. Leave as null if using the automatical filling with `--pfam_version`.",
                     "fa_icon": "fas fa-link"
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 8d2657ab4..94b2ac5c8 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -64,9 +64,7 @@ workflow PIPELINE_INITIALISATION {
     //
     // Custom validation for pipeline parameters
     //
-    validateInputParameters(
-
-    )
+    validateInputParameters()
 
     //
     // Create channel from input file provided through params.input

From b6f4cace1cb921429b009de4f3b3a9f5de5f158f Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Thu, 17 Jul 2025 18:00:34 +0000
Subject: [PATCH 667/887] Add error if neither dfam params nor dfam version and
 species are specified

---
 subworkflows/local/build_references/main.nf | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 852185390..3458ca89d 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -184,12 +184,14 @@ workflow BUILD_REFERENCES {
                 dfam_h3i = Channel.fromPath(params.dfam_h3i, checkIfExists: true)
                 dfam_h3m = Channel.fromPath(params.dfam_h3m, checkIfExists: true)
                 dfam_h3p = Channel.fromPath(params.dfam_h3p, checkIfExists: true)
-            } else {
+            } else if(params.dfam_version && params.species) {
                 dfam_hmm = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"    , checkIfExists: true)
                 dfam_h3f = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f", checkIfExists: true)
                 dfam_h3i = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i", checkIfExists: true)
                 dfam_h3m = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m", checkIfExists: true)
                 dfam_h3p = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p", checkIfExists: true)
+            } else {
+                error("Expected --dfam_version and --species to be specified when using StarFusion to automatically fill in Dfam database or specify --dfam_{hmm,h3f,h3i,h3m,h3p} for manual input")
             }
 
             dfam_urls_ch = dfam_hmm

From 29d8af104d4ec77501ac31f19bb3d0a36134cafc Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Thu, 17 Jul 2025 18:08:49 +0000
Subject: [PATCH 668/887] Make warning message clearer

---
 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 94b2ac5c8..075429798 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -163,7 +163,7 @@ def validateInputParameters() {
     }
 
     if ((params.dfam_hmm || params.dfam_h3p || params.dfam_h3m || params.dfam_h3i || params.dfam_h3f) && (params.dfam_version)) {
-        log.warn("Both custom dfam_urls and dfam_version were specified. \n If you want to use custom dfam URLs make sure to provide the full paths for each of the dfam params as the dfam_version will not be overwritten. \n Otherwise, use only the `--dfam_version` and `--species` params and the dfam URLs will be automatically filled.")
+        log.warn("Both custom dfam_urls and dfam_version were specified. URL building based on --dfam_version will not be triggered, instead specified dfam_urls will be used. \n If you want to use custom dfam URLs make sure to provide the full paths for each of the dfam params as the dfam_version will not be used. \n Otherwise, use only the `--dfam_version` and `--species` params and the dfam URLs will be automatically filled.")
     }
 
 }

From eac2b9b4cb7f28e6e7ec2fad38305b3508120be1 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Thu, 17 Jul 2025 18:40:05 +0000
Subject: [PATCH 669/887] Update test_build

---
 tests/test_build.nf.test.snap | 146 +++++++++++++++++++---------------
 1 file changed, 81 insertions(+), 65 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 01b0c09af..831498f18 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -63,7 +63,6 @@
                 "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
                 "references/fusion_report_db",
                 "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
                 "references/fusion_report_db/fusion_report.log",
                 "references/fusion_report_db/fusiongdb2.db",
                 "references/fusion_report_db/mitelman.db",
@@ -188,42 +187,42 @@
                 "references/starfusion/minigenome.fa_genome_lib_build_dir/trans.blast.dat.gz"
             ],
             [
-                "minigenome.dict:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
+                "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.gtf.interval_list:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.gtf.refflat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.gtf.fasta:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "complete_ref_lens.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ctg_offsets.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "duplicate_clusters.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "mphf.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "rank.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refAccumLengths.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "reflengths.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refseq.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "versionInfo.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
+                "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
+                "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
+                "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
+                "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
+                "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
+                "info.json:md5,d91d8a954afcb4be9fe7bfb3e84b5be6",
+                "mphf.bin:md5,b364192c6c73de4c38ec2ed0750f5155",
+                "rank.bin:md5,3fe9c2dc9da7faff6362510c4f29e87d",
+                "refAccumLengths.bin:md5,26d9405e7591ec9a606caa36a094941a",
+                "reflengths.bin:md5,187a4a0dee316c5dbf440fa3c58d9eec",
+                "refseq.bin:md5,712c4a8d988c15016ea746db7022e549",
+                "versionInfo.json:md5,d2c799050e81aa6e282ac8a73e773941",
+                "Genome:md5,1621486e3b854fefa37c0b4e77275233",
+                "SA:md5,9b6b936f3a8a1232c4fc5533daf9401d",
+                "SAindex:md5,8b2025227b78fdc83e3e5b17c0f538f7",
+                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "sjdbInfo.txt:md5,f044cb1cd38af66c832f3afc4b1655cb",
+                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+                "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
+                "PFAM.domtblout.dat.gz:md5,54f921cd70902acb83ff5b14aebd604a",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -247,42 +246,59 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.dat.gz:md5,ebc8f093656956b218544cc3d6c714e6",
+                "blast_pairs.idx:md5,bc62513f72fe691d044b40450a2f759d",
+                "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                "fusion_annot_lib.idx:md5,6d5f914c3ef873129864704ace7b6023",
+                "pfam_domains.dbm:md5,fa101bda7d051bcd3f5943ed241d512f",
+                "ref_annot.cdna.fa:md5,dae68f2c6b2b910fc7fba7be851ff8aa",
+                "ref_annot.cdna.fa.idx:md5,49a67627a7738284db86cf5315f4581a",
+                "ref_annot.cds:md5,c194a1e03db28d0e6e9c2fda8b57b2d3",
+                "ref_annot.cdsplus.fa:md5,1be8e201b908d0d1e3b67588a295e092",
+                "ref_annot.cdsplus.fa.idx:md5,b75b353e4fe32628d243f1bb6cc293d6",
+                "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                "ref_annot.gtf.gene_spans:md5,5b3b025a5ac4fe18273e591227b807c6",
+                "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+                "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                "ref_annot.pep:md5,2a5ba213d81f6b0215a9a5b56ee5243a",
+                "ref_annot.prot_info.dbm:md5,353f077ac7d0d9aea47aac057e706953",
+                "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+                "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+                "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+                "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                "ref_genome.fa.nin:md5,db10a8b187c7cdb0c36a93424d15f07c",
+                "ref_genome.fa.njs:md5,60536e1c49690c3d8bb76370219b08e1",
+                "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+                "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+                "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+                "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+                "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                "Log.out:md5,43ee8d90b889391994d21a684dd85104",
+                "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+                "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "genomeParameters.txt:md5,33d7b726ef158db446859f89c8c3525f",
+                "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+                "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                "trans.blast.align_coords.align_coords.dbm:md5,edc8b34f6f5057414f947950efa48175",
+                "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T15:25:05.434296"
+        "timestamp": "2025-07-17T18:35:50.278173296"
     }
-}
+}
\ No newline at end of file

From eaa140dcba1d7cf41d94656066dc802332d9de10 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Thu, 17 Jul 2025 20:22:46 +0000
Subject: [PATCH 670/887] Fix test_build

---
 tests/.nftignore              | 38 +++++++++++++++++------------------
 tests/test_build.nf.test      | 15 +-------------
 tests/test_build.nf.test.snap | 20 +-----------------
 3 files changed, 21 insertions(+), 52 deletions(-)

diff --git a/tests/.nftignore b/tests/.nftignore
index c11f3e9d4..033f0488b 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -13,25 +13,25 @@ references/salmon/salmon/pre_indexing.log
 references/salmon/salmon/ref_indexing.log
 references/salmon/salmon/seq.bin
 references/star/Log.out
-references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/
-references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz
-references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz
-references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx
-references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx
-references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep
-references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm
-references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin
-references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs
-references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
-references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
+references/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/
+references/starfusion/*_genome_lib_build_dir/PFAM.domtblout.dat.gz
+references/starfusion/*_genome_lib_build_dir/blast_pairs.dat.gz
+references/starfusion/*_genome_lib_build_dir/blast_pairs.idx
+references/starfusion/*_genome_lib_build_dir/fusion_annot_lib.idx
+references/starfusion/*_genome_lib_build_dir/pfam_domains.dbm
+references/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa
+references/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa.idx
+references/starfusion/*_genome_lib_build_dir/ref_annot.cds
+references/starfusion/*_genome_lib_build_dir/ref_annot.cdsplus.fa
+references/starfusion/*_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
+references/starfusion/*_genome_lib_build_dir/ref_annot.gtf.gene_spans
+references/starfusion/*_genome_lib_build_dir/ref_annot.pep
+references/starfusion/*_genome_lib_build_dir/ref_annot.prot_info.dbm
+references/starfusion/*_genome_lib_build_dir/ref_genome.fa.nin
+references/starfusion/*_genome_lib_build_dir/ref_genome.fa.njs
+references/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
+references/starfusion/*_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
 references/star/genomeParameters.txt
-references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
+references/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
 references/fusion_report_db/mitelman.db
 references/fusion_report_db/fusiongdb2.db
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 0f5387d03..df43626a9 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -11,20 +11,7 @@ nextflow_pipeline {
 
         when {
             params {
-                config_profile_name        = 'Test build references profile'
-                config_profile_description = 'Minimal test dataset to check pipeline function'
-
-                // Input data
-                references_only            = true
-                input                      = "${projectDir}/tests/csv/fastq.csv"
-                no_cosmic                  = true
-                tools                      = "arriba,starfusion,fusionreport,salmon,fusioninspector"
-                fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'
-                gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
-                fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
-                species                    = 'homo_sapiens'
-                dfam_version               = 3.4
-                outdir                     = "$outputDir"
+                outdir = "$outputDir"
             }
         }
 
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 831498f18..9e5959903 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -222,7 +222,6 @@
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-                "PFAM.domtblout.dat.gz:md5,54f921cd70902acb83ff5b14aebd604a",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -246,35 +245,20 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.dat.gz:md5,ebc8f093656956b218544cc3d6c714e6",
-                "blast_pairs.idx:md5,bc62513f72fe691d044b40450a2f759d",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                "fusion_annot_lib.idx:md5,6d5f914c3ef873129864704ace7b6023",
-                "pfam_domains.dbm:md5,fa101bda7d051bcd3f5943ed241d512f",
-                "ref_annot.cdna.fa:md5,dae68f2c6b2b910fc7fba7be851ff8aa",
-                "ref_annot.cdna.fa.idx:md5,49a67627a7738284db86cf5315f4581a",
-                "ref_annot.cds:md5,c194a1e03db28d0e6e9c2fda8b57b2d3",
-                "ref_annot.cdsplus.fa:md5,1be8e201b908d0d1e3b67588a295e092",
-                "ref_annot.cdsplus.fa.idx:md5,b75b353e4fe32628d243f1bb6cc293d6",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                "ref_annot.gtf.gene_spans:md5,5b3b025a5ac4fe18273e591227b807c6",
                 "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                 "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                "ref_annot.pep:md5,2a5ba213d81f6b0215a9a5b56ee5243a",
-                "ref_annot.prot_info.dbm:md5,353f077ac7d0d9aea47aac057e706953",
                 "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                 "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                 "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                 "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                "ref_genome.fa.nin:md5,db10a8b187c7cdb0c36a93424d15f07c",
-                "ref_genome.fa.njs:md5,60536e1c49690c3d8bb76370219b08e1",
                 "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                 "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                 "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                 "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                "Log.out:md5,43ee8d90b889391994d21a684dd85104",
                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -285,13 +269,11 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "genomeParameters.txt:md5,33d7b726ef158db446859f89c8c3525f",
                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                "trans.blast.align_coords.align_coords.dbm:md5,edc8b34f6f5057414f947950efa48175",
                 "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
             ]
         ],
@@ -301,4 +283,4 @@
         },
         "timestamp": "2025-07-17T18:35:50.278173296"
     }
-}
\ No newline at end of file
+}

From dd76a94491f4bf6805ddc8a8fc972ee3a7b247be Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 18 Jul 2025 09:42:13 +0200
Subject: [PATCH 671/887] remove includes of download processes

---
 subworkflows/local/build_references/main.nf | 2 --
 1 file changed, 2 deletions(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 3ee8e9a2b..77ef7c331 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -5,7 +5,6 @@
 */
 
 include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode_download/main'
-include { FUSIONCATCHER_DOWNLOAD }          from '../../../modules/local/fusioncatcher/download/main'
 include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/nf-core/fusionreport/download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
 include { STARFUSION_BUILD }                from '../../../modules/local/starfusion/build/main'
@@ -19,7 +18,6 @@ include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatspl
 ========================================================================================
 */
 
-include { ARRIBA_DOWNLOAD }                 from '../../../modules/nf-core/arriba/download/main'
 include { SAMTOOLS_FAIDX }                  from '../../../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../../../modules/nf-core/star/genomegenerate/main'
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../../modules/nf-core/gatk4/createsequencedictionary/main'

From 951ae480ceda2c6c4ae33946c3935a73fef143e7 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 18 Jul 2025 09:50:29 +0200
Subject: [PATCH 672/887] fix some error messages

---
 workflows/rnafusion.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 0d4666aed..bb7199513 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -310,7 +310,7 @@ workflow RNAFUSION {
         def ch_fusionreport_csv = Channel.empty()
         if(!params.skip_vis && tools.contains("fusionreport")) {
             if (!fusions_created) {
-                error("Could not find any fusion files. Please generate some with --arriba, --starfusion and/or --fusioncatcher")
+                error("Could not find any fusion files. Please generate some with `--tools arriba`, `--tools starfusion` and/or `--tools fusioncatcher`")
             }
             FUSIONREPORT_WORKFLOW (
                 BUILD_REFERENCES.out.fusionreport_ref,
@@ -330,7 +330,7 @@ workflow RNAFUSION {
             ch_fusionreport_csv     = null
             ch_fusionreport_report  = null
         } else if(tools.contains("fusioninspector")) {
-            error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with --arriba, --starfusion and/or --fusioncatcher with --skip_vis disabled and --fusionreport enabled")
+            error("Could not find any valid fusions for fusioninspector input. Please provide some via --fusioninspector_fusions or generate them with `--tools arriba`, `--tools starfusion` and/or `--tools fusioncatcher` with --skip_vis disabled and `--tools fusionreport enabled")
         }
 
         //

From a5c7163da35b493839cc7829149ec2d33db85581 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 18 Jul 2025 09:51:09 +0200
Subject: [PATCH 673/887] add a debug for tools to run

---
 main.nf | 1 +
 1 file changed, 1 insertion(+)

diff --git a/main.nf b/main.nf
index 2408f9c2c..8561d70db 100644
--- a/main.nf
+++ b/main.nf
@@ -72,6 +72,7 @@ workflow {
         }
         tools = pattern.replace('^((', "").replace(')?,?)*(?<!,)$', "").tokenize("|") - "all"
     }
+    log.debug("Rnafusion tools to run: ${tools}")
 
     def profiles = workflow.profile
     if ((profiles.contains("conda") || profiles.contains("mamba")) && (tools.contains("ctatsplicing"))) {

From 6ffdddfe91a0f5b673b50297711d1569f90cdea4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 18 Jul 2025 14:18:34 +0200
Subject: [PATCH 674/887] add creation of missing output files

---
 modules/local/ctatsplicing/startocancerintrons/main.nf | 8 ++++++++
 1 file changed, 8 insertions(+)

diff --git a/modules/local/ctatsplicing/startocancerintrons/main.nf b/modules/local/ctatsplicing/startocancerintrons/main.nf
index a8d683ec2..4681bf783 100644
--- a/modules/local/ctatsplicing/startocancerintrons/main.nf
+++ b/modules/local/ctatsplicing/startocancerintrons/main.nf
@@ -41,6 +41,14 @@ process CTATSPLICING_STARTOCANCERINTRONS {
         --ctat_genome_lib ${genome_lib} \\
         ${args}
 
+    # Create the missing outputs when no cancer introns are found
+    if [ $? -eq 0 ]; then
+        touch ${prefix}.cancer_intron_reads.sorted.bam
+        touch ${prefix}.cancer_intron_reads.sorted.bam.bai
+        touch ${prefix}.gene_reads.sorted.sifted.bam
+        touch ${prefix}.gene_reads.sorted.sifted.bam.bai
+    fi
+
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         ctat-splicing: $VERSION

From c9f0b78ff7068a01053f9e421f2f0cde84d9c4da Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 18 Jul 2025 14:29:50 +0200
Subject: [PATCH 675/887] fix + changelog

---
 CHANGELOG.md                                           | 1 +
 modules/local/ctatsplicing/startocancerintrons/main.nf | 2 +-
 2 files changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4383082d3..752d565a2 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -105,6 +105,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix missing memory unit for fusioncatcher [#674](https://github.com/nf-core/rnafusion/pull/674)
 - Fix fusioncatcher download link [#693](https://github.com/nf-core/rnafusion/pull/693)
 - Fix fusionreport singularity container [#713](https://github.com/nf-core/rnafusion/pull/713)
+- Fix CTAT-SPLICING output when no cancer introns were found [#722](https://github.com/nf-core/rnafusion/pull/722)
 
 ### Removed
 
diff --git a/modules/local/ctatsplicing/startocancerintrons/main.nf b/modules/local/ctatsplicing/startocancerintrons/main.nf
index 4681bf783..3d7e26027 100644
--- a/modules/local/ctatsplicing/startocancerintrons/main.nf
+++ b/modules/local/ctatsplicing/startocancerintrons/main.nf
@@ -42,7 +42,7 @@ process CTATSPLICING_STARTOCANCERINTRONS {
         ${args}
 
     # Create the missing outputs when no cancer introns are found
-    if [ $? -eq 0 ]; then
+    if [ \$? -eq 0 ]; then
         touch ${prefix}.cancer_intron_reads.sorted.bam
         touch ${prefix}.cancer_intron_reads.sorted.bam.bai
         touch ${prefix}.gene_reads.sorted.sifted.bam

From 97ccaab5c3d9f8820b3b8761cddde9d7d203d0ea Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 18 Jul 2025 14:51:37 +0200
Subject: [PATCH 676/887] fix pre-commit

---
 modules.json | 126 +++++++++++++--------------------------------------
 1 file changed, 32 insertions(+), 94 deletions(-)

diff --git a/modules.json b/modules.json
index b7a33c03f..4e91ce69e 100644
--- a/modules.json
+++ b/modules.json
@@ -8,198 +8,142 @@
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "b1966f36ec9de31927b2603d8f499960b2a4c294",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "1037205706929921360f57e8042eae6819825e31",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fusionreport/detect": {
                         "branch": "master",
                         "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fusionreport/download": {
                         "branch": "master",
                         "git_sha": "df4b662dae05afb695a4326fc172178b5a7a48ce",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "1ec937ab3edc307bc0d79a2200d784e9f0868359",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "7b50cb7be890e4b28cffb82e438cc6a8d7805d3f",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "980a4964be91da75fb3ccf576118a18e247f6d15",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/convert": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     }
                 }
             },
@@ -208,26 +152,20 @@
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From 437413d6f4f0735feccf2c6fb513092b97af3493 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 18 Jul 2025 14:54:14 +0200
Subject: [PATCH 677/887] fix nf-core lint

---
 conf/modules.config  | 16 ----------------
 nextflow_schema.json |  2 +-
 2 files changed, 1 insertion(+), 17 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 95331646b..f7e712a93 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -27,14 +27,6 @@ process {
         ext.prefix =  { "${meta.id}.arriba" }
     }
 
-    withName: 'ARRIBA_DOWNLOAD' {
-        publishDir = [
-            path: { "${params.genomes_base}/arriba" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
     withName: 'ARRIBA_VISUALISATION' {
         ext.prefix = { "${meta.id}_combined_fusions_arriba_visualisation" }
         publishDir = [
@@ -110,14 +102,6 @@ process {
         ].join(' ')}
     }
 
-    withName: 'FUSIONCATCHER_DOWNLOAD' {
-        publishDir = [
-            path: { "${params.genomes_base}/fusioncatcher" },
-            mode: params.publish_dir_mode,
-            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
-        ]
-    }
-
     withName: 'FUSIONINSPECTOR' {
         ext.args = { params.fusioninspector_limitSjdbInsertNsj != 1000000 ? "--STAR_xtra_params \"--limitSjdbInsertNsj ${params.fusioninspector_limitSjdbInsertNsj}\"" : '' }
         ext.args2 = '--annotate --examine_coding_effect'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index ba3a60d88..3c134add6 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -77,7 +77,7 @@
                     "description": "Path to reference folder"
                 },
                 "genomes_ignore": {
-                    "type": "string",
+                    "type": "boolean",
                     "fa_icon": "far fa-file-code",
                     "description": "Don't automatically assign reference parameters to the correct references in --genomes_base"
                 },

From 97a48cde3e5b916b34d0614ddaeca2e7a6a0a004 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 18 Jul 2025 15:22:24 +0200
Subject: [PATCH 678/887] Update nf-test.yml
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Co-authored-by: Júlia Mir Pedrol <mirp.julia@gmail.com>
---
 .github/workflows/nf-test.yml | 1 +
 1 file changed, 1 insertion(+)

diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index 9ec706eca..8021852c8 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -64,6 +64,7 @@ jobs:
     runs-on: # use self-hosted runners
       - runs-on=${{ github.run_id }}-nf-test
       - runner=4cpu-linux-x64
+      - disk=large
     strategy:
       fail-fast: false
       matrix:

From 90a9bf8ddfa40dbed68d32686473fcf750703f39 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Fri, 18 Jul 2025 17:56:04 +0000
Subject: [PATCH 679/887] Fix: Allow custom pfam and dfam files without
 harcoding

---
 conf/test_build.config                        |  7 +++
 nextflow.config                               | 18 ++++----
 nextflow_schema.json                          | 44 +++++++++----------
 subworkflows/local/build_references/main.nf   | 16 +++----
 .../utils_nfcore_rnafusion_pipeline/main.nf   | 36 ++++++++++++++-
 tests/test_stub.nf.test                       |  2 +-
 6 files changed, 78 insertions(+), 45 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index 3ba2891b1..15178782d 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -44,6 +44,13 @@ params {
     fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
     species                    = 'homo_sapiens'
     dfam_version               = 3.4
+    pfam_version               = 37.4
+    dfam_hmm                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"
+    dfam_h3f                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f"
+    dfam_h3i                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i"
+    dfam_h3m                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m"
+    dfam_h3p                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p"
+    pfam_file                  = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"
 
 }
 
diff --git a/nextflow.config b/nextflow.config
index 645587b2a..4c9417508 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -31,9 +31,6 @@ params {
     read_length                = 100
     genomes                    = [:]
     fusion_annot_lib           = "https://github.com/FusionAnnotator/CTAT_HumanFusionLib/releases/download/v0.3.0/fusion_lib.Mar2021.dat.gz" // path to  dat.gz CTAT genome lib // TODO: Update to latest with s3 link when available
-    species                    = "homo_sapiens"
-    dfam_version               = 3.8
-    pfam_version               = 37.4
 
     // Filtering
     tools_cutoff               = 1
@@ -85,12 +82,15 @@ params {
     starindex_ref                 = "${params.genomes_base}/star"
     fusionreport_ref              = "${params.genomes_base}/fusion_report_db"
     ctatsplicing_cancer_introns   = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
-    dfam_hmm                      = null
-    dfam_h3f                      = null
-    dfam_h3i                      = null
-    dfam_h3m                      = null
-    dfam_h3p                      = null
-    pfam_url                      = null
+    species                       = "homo_sapiens"
+    dfam_version                  = 3.8
+    pfam_version                  = 37.4
+    dfam_hmm                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"
+    dfam_h3f                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f"
+    dfam_h3i                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i"
+    dfam_h3m                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m"
+    dfam_h3p                      = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p"
+    pfam_file                     = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"
     annot_filter_url              = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
 
     // Path to fusion outputs
diff --git a/nextflow_schema.json b/nextflow_schema.json
index deee57b44..90af4f1c3 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -232,50 +232,50 @@
                 },
                 "dfam_hmm": {
                     "type": "string",
-                    "format": "uri",
+                    "format": "file-path",
                     "pattern": "\\S+\\.hmm$",
-                    "description": "URL to Dfam HMM database file",
-                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam HMM database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "description": "Path to Dfam HMM database file",
+                    "help_text": "Provide a valid path pointing to the Dfam HMM database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
                 },
                 "dfam_h3f": {
                     "type": "string",
-                    "format": "uri",
+                    "format": "file-path",
                     "pattern": "\\S+\\.h3f$",
-                    "description": "URL to Dfam H3F database file",
-                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3F database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "description": "Path to Dfam H3F database file",
+                    "help_text": "Provide a valid path pointing to the Dfam H3F database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
                 },
                 "dfam_h3i": {
                     "type": "string",
-                    "format": "uri",
+                    "format": "file-path",
                     "pattern": "\\S+\\.h3i$",
-                    "description": "URL to Dfam H3I database file",
-                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3I database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "description": "Path to Dfam H3I database file",
+                    "help_text": "Provide a valid path pointing to the Dfam H3I database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
                 },
                 "dfam_h3m": {
                     "type": "string",
-                    "format": "uri",
+                    "format": "file-path",
                     "pattern": "\\S+\\.h3m$",
-                    "description": "URL to Dfam H3M database file",
-                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3M database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "description": "Path to Dfam H3M database file",
+                    "help_text": "Provide a valid path pointing to the Dfam H3M database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
                 },
                 "dfam_h3p": {
                     "type": "string",
-                    "format": "uri",
+                    "format": "file-path",
                     "pattern": "\\S+\\.h3p$",
-                    "description": "URL to Dfam H3P database file",
-                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Dfam H3P database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
+                    "description": "Path to Dfam H3P database file",
+                    "help_text": "Provide a valid path pointing to the Dfam H3P database file. Leave as null if using the automatical filling with `--dfam_version` and `--species`.",
                     "fa_icon": "fas fa-link"
                 },
-                "pfam_url": {
+                "pfam_file": {
                     "type": "string",
-                    "format": "uri",
-                    "pattern": "\\S+\\.hmm(\\.gz)$",
-                    "description": "URL to Pfam database file",
-                    "help_text": "Provide a valid HTTP/HTTPS URL pointing to the Pfam database file. Leave as null if using the automatical filling with `--pfam_version`.",
+                    "format": "file-path",
+                    "pattern": "\\S+\\.hmm(\\.gz)?$",
+                    "description": "Path to Pfam database file",
+                    "help_text": "Provide a valid path pointing to the Pfam database file. Leave as null if using the automatical filling with `--pfam_version`.",
                     "fa_icon": "fas fa-link"
                 },
                 "annot_filter_url": {
@@ -424,11 +424,11 @@
                     "default": "homo_sapiens"
                 },
                 "dfam_version": {
-                    "type": "number",
+                    "type": ["number", "null"],
                     "description": "Version of dfam to use"
                 },
                 "pfam_version": {
-                    "type": "number",
+                    "type": ["number", "null"],
                     "description": "Version of pfam to use"
                 }
             }
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 3458ca89d..2ac9141f6 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -172,10 +172,10 @@ workflow BUILD_REFERENCES {
                 error("Expected --fusion_annot_lib to be specified when using StarFusion or any tools that depend on it")
             }
 
-            if(params.pfam_url) {
-                pfam_url = Channel.fromPath(params.pfam_url, checkIfExists: true)
+            if(params.pfam_file) {
+                pfam_file = Channel.fromPath(params.pfam_file, checkIfExists: true)
             } else {
-                pfam_url = Channel.fromPath("http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz", checkIfExists: true)
+                error("Expected `--pfam_version` to be specified when using StarFusion to automatically fill in Pfam database or specify `--pfam_file` for custom input")
             }
 
             if(params.dfam_hmm && params.dfam_h3p && params.dfam_h3m && params.dfam_h3i && params.dfam_h3f) {
@@ -184,14 +184,8 @@ workflow BUILD_REFERENCES {
                 dfam_h3i = Channel.fromPath(params.dfam_h3i, checkIfExists: true)
                 dfam_h3m = Channel.fromPath(params.dfam_h3m, checkIfExists: true)
                 dfam_h3p = Channel.fromPath(params.dfam_h3p, checkIfExists: true)
-            } else if(params.dfam_version && params.species) {
-                dfam_hmm = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"    , checkIfExists: true)
-                dfam_h3f = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f", checkIfExists: true)
-                dfam_h3i = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i", checkIfExists: true)
-                dfam_h3m = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m", checkIfExists: true)
-                dfam_h3p = Channel.fromPath("https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p", checkIfExists: true)
             } else {
-                error("Expected --dfam_version and --species to be specified when using StarFusion to automatically fill in Dfam database or specify --dfam_{hmm,h3f,h3i,h3m,h3p} for manual input")
+                error("Expected `--dfam_version` and `--species` to be specified when using StarFusion to automatically fill in Dfam database or specify `--dfam_{hmm,h3f,h3i,h3m,h3p}` for custom input")
             }
 
             dfam_urls_ch = dfam_hmm
@@ -203,7 +197,7 @@ workflow BUILD_REFERENCES {
                 )
                 .collect()
 
-            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, pfam_url, dfam_urls_ch, params.annot_filter_url)
+            STARFUSION_BUILD(ch_fasta, ch_gtf, params.fusion_annot_lib, params.species, pfam_file, dfam_urls_ch, params.annot_filter_url)
             ch_versions = ch_versions.mix(STARFUSION_BUILD.out.versions)
             if (tools.contains("ctatsplicing")) {
                 CTATSPLICING_PREPGENOMELIB(
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 075429798..b89988f4e 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -162,10 +162,42 @@ def validateInputParameters() {
         log.warn("Skipping COSMIC DB download from `FUSIONREPORT_DOWNLOAD` and skip using it in `FUSIONREPORT`")
     }
 
-    if ((params.dfam_hmm || params.dfam_h3p || params.dfam_h3m || params.dfam_h3i || params.dfam_h3f) && (params.dfam_version)) {
-        log.warn("Both custom dfam_urls and dfam_version were specified. URL building based on --dfam_version will not be triggered, instead specified dfam_urls will be used. \n If you want to use custom dfam URLs make sure to provide the full paths for each of the dfam params as the dfam_version will not be used. \n Otherwise, use only the `--dfam_version` and `--species` params and the dfam URLs will be automatically filled.")
+    def dfamParams = ['dfam_hmm', 'dfam_h3p', 'dfam_h3m', 'dfam_h3i', 'dfam_h3f']
+
+    if (params.dfam_version) {
+        def dfamPattern = /https:\/\/www\.dfam\.org\/releases\/Dfam_${params.dfam_version}\/infrastructure\/dfamscan\/${params.species}_dfam\..*/
+
+        def setDfamParams = dfamParams.findAll { params[it] }
+
+        if (setDfamParams) {
+            def customParams = setDfamParams.findAll { paramName ->
+                !params[paramName].matches(dfamPattern)
+            }
+            if (customParams) {
+                def paramDetails = customParams.collect { paramName ->
+                    "   --${paramName}: ${params[paramName]}"
+                }.join('\n')
+                dfam_warn = "Both custom DFAM paths as well as `--dfam_version` (${params.dfam_version}) and `--species` (${params.species}) were provided.\n" +
+                    "Custom DFAM paths parameters provided:\n${paramDetails}\n" +
+                    "The pipeline will prioritize these custom files specified with `--${customParams}` and **will NOT** construct these URLs based on `--dfam_version` nor `--species`.\n" +
+                    "   - If you intend to use custom DFAM files, please ensure that all `--dfam_h*` parameters point to full and valid paths.\n" +
+                    "   - If you prefer to let the pipeline build the DFAM URLs automatically, omit `--dfam_h*` and instead provide only `--dfam_version` and `--species`."
+                log.warn(dfam_warn)
+            }
+        }
     }
 
+    if (params.pfam_version){
+        def pfamPattern = /http:\/\/ftp\.ebi\.ac\.uk\/pub\/databases\/Pfam\/releases\/Pfam${params.pfam_version}\/Pfam-A\..*/
+
+        if (!(params.pfam_file.matches(pfamPattern))){
+            pfam_warn = "Both `--pfam_file` (${params.pfam_file}) and `--pfam_version` (${params.pfam_version}) were provided.\n" +
+                    "The pipeline will prioritize the custom file from `--pfam_file` and **will NOT** construct the URL based on `--pfam_version`.\n" +
+                    "   - If you intend to use a custom PFAM file, please ensure that `--pfam_file` points to a full and valid path.\n" +
+                    "   - If you prefer to let the pipeline build the PFAM URL automatically, omit `--pfam_file` and instead provide only `--pfam_version`."
+            log.warn(pfam_warn)
+        }
+    }
 }
 
 //
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 495d20b3d..804f36166 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -15,7 +15,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 tools            = "all"
-                no_cosmic              = true
+                no_cosmic        = true
             }
         }
 

From bc8e64c12c325ca2055dd5d6b3e6f383792b2a45 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Fri, 18 Jul 2025 18:03:19 +0000
Subject: [PATCH 680/887] Update `CHANGELOG.md`

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4383082d3..462de3180 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -46,6 +46,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update STAR-Fusion to 1.15.0 and update nf-core modules [#664](https://github.com/nf-core/rnafusion/pull/664)
 - Update fusionreport 4.0.0 that replaces the score of a fusion with a Fusion Indication Index [#667](https://github.com/nf-core/rnafusion/pull/667)
 - Added a extra trimming step for fusioncatcher in case a different read-length is wished for this tool only [#674](https://github.com/nf-core/rnafusion/pull/674)
+- Added `--dfam_hmm`, `--dfam_h3f`, `--dfam_h3i`, `--dfam_h3m`, `--dfam_h3p`, `--pfam_file` and `--annot_filter_url` parameters to allow use of custom files in `STARFUSION_BUILD` module [#709](https://github.com/nf-core/rnafusion/pull/709)
 
 ### Changed
 
@@ -85,6 +86,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Replaced local `FUSIONREPORT` and `FUSIONREPORT_DOWNLOAD` with `FUSIONREPORT_DETECT` and `FUSIONREPORT_DOWNLOAD` from nf-core [#703](https://github.com/nf-core/rnafusion/pull/703)
 - Updated arriba to v2.5.0 [#693](https://github.com/nf-core/rnafusion/pull/693)
 - Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
+- Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
 
 ### Fixed
 

From 55c621c4801d8601c4a653e8056fef9a9926aadb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Jul 2025 13:21:07 +0200
Subject: [PATCH 681/887] update qc_workflow snap

---
 .../local/qc_workflow/test/main.nf.test.snap  | 32 +++++++++++++++++++
 1 file changed, 32 insertions(+)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index b56c12b98..cdaa0cc8b 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -17,6 +17,38 @@
         },
         "timestamp": "2024-12-04T19:29:09.686297468"
     },
+    "QC_WORKFLOW - Homo sapiens chr22": {
+        "content": [
+            [
+                "## METRICS CLASS\tpicard.analysis.RnaSeqMetrics",
+                "PF_BASES\tPF_ALIGNED_BASES\tRIBOSOMAL_BASES\tCODING_BASES\tUTR_BASES\tINTRONIC_BASES\tINTERGENIC_BASES\tIGNORED_READS\tCORRECT_STRAND_READS\tINCORRECT_STRAND_READS\tNUM_R1_TRANSCRIPT_STRAND_READS\tNUM_R2_TRANSCRIPT_STRAND_READS\tNUM_UNEXPLAINED_READS\tPCT_R1_TRANSCRIPT_STRAND_READS\tPCT_R2_TRANSCRIPT_STRAND_READS\tPCT_RIBOSOMAL_BASES\tPCT_CODING_BASES\tPCT_UTR_BASES\tPCT_INTRONIC_BASES\tPCT_INTERGENIC_BASES\tPCT_MRNA_BASES\tPCT_USABLE_BASES\tPCT_CORRECT_STRAND_READS\tMEDIAN_CV_COVERAGE\tMEDIAN_5PRIME_BIAS\tMEDIAN_3PRIME_BIAS\tMEDIAN_5PRIME_TO_3PRIME_BIAS\tSAMPLE\tLIBRARY\tREAD_GROUP",
+                "672131\t670989\t0\t0\t323630\t84236\t263123\t0\t1324\t1328\t664\t661\t1\t0.501132\t0.498868\t0\t0\t0.482318\t0.12554\t0.392142\t0.482318\t0.481498\t0.499246\t4.318717\t0\t0.529614\t0\t\t\t",
+                ""
+            ],
+            [
+                "## METRICS CLASS\tpicard.sam.DuplicationMetrics",
+                "LIBRARY\tUNPAIRED_READS_EXAMINED\tREAD_PAIRS_EXAMINED\tSECONDARY_OR_SUPPLEMENTARY_RDS\tUNMAPPED_READS\tUNPAIRED_READ_DUPLICATES\tREAD_PAIR_DUPLICATES\tREAD_PAIR_OPTICAL_DUPLICATES\tPERCENT_DUPLICATION\tESTIMATED_LIBRARY_SIZE",
+                "testN\t0\t2820\t2\t2\t0\t828\t0\t0.293617\t3807",
+                ""
+            ],
+            [
+                "## METRICS CLASS\tpicard.analysis.InsertSizeMetrics",
+                "MEDIAN_INSERT_SIZE\tMODE_INSERT_SIZE\tMEDIAN_ABSOLUTE_DEVIATION\tMIN_INSERT_SIZE\tMAX_INSERT_SIZE\tMEAN_INSERT_SIZE\tSTANDARD_DEVIATION\tREAD_PAIRS\tPAIR_ORIENTATION\tWIDTH_OF_10_PERCENT\tWIDTH_OF_20_PERCENT\tWIDTH_OF_30_PERCENT\tWIDTH_OF_40_PERCENT\tWIDTH_OF_50_PERCENT\tWIDTH_OF_60_PERCENT\tWIDTH_OF_70_PERCENT\tWIDTH_OF_80_PERCENT\tWIDTH_OF_90_PERCENT\tWIDTH_OF_95_PERCENT\tWIDTH_OF_99_PERCENT\tSAMPLE\tLIBRARY\tREAD_GROUP",
+                "123\t96\t23\t32\t300\t125.684397\t32.447863\t2820\tFR\t11\t17\t27\t37\t47\t57\t69\t83\t103\t125\t169\t\t\t",
+                ""
+            ],
+            [
+                "versions.yml:md5,43d6a3cb756a214f3956bd5c740cb26a",
+                "versions.yml:md5,5c0ffe79bb7206c2652cf7f477f9b53f",
+                "versions.yml:md5,9951386776792bef3fd644ef9da67e7a"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-22T13:19:46.99615"
+    },
     "rnaseq_metrics": {
         "content": "84a348c3735ed2f6c47f346eeed661f4",
         "meta": {

From 5b696f248d0c4e62f578a15b94af5f622b961fe0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Jul 2025 13:25:06 +0200
Subject: [PATCH 682/887] update hgnc snap

---
 modules/local/hgnc/tests/main.nf.test.snap | 14 ++++----------
 1 file changed, 4 insertions(+), 10 deletions(-)

diff --git a/modules/local/hgnc/tests/main.nf.test.snap b/modules/local/hgnc/tests/main.nf.test.snap
index 1fd34b6a1..87dab9036 100644
--- a/modules/local/hgnc/tests/main.nf.test.snap
+++ b/modules/local/hgnc/tests/main.nf.test.snap
@@ -1,19 +1,13 @@
 {
     "Should create stub files": {
         "content": [
-            [
-                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
-            ],
-            "HGNC-DB-timestamp.txt",
-            [
-                "versions.yml:md5,a7b7ccbd9eda8036baf548cdf1cb6867"
-            ]
+            "HGNC-DB-timestamp.txt"
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.1"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-11-26T17:42:04.47884487"
+        "timestamp": "2025-07-22T13:23:08.771393"
     },
     "Should download HGNC files": {
         "content": [

From dea24e621a9eb0b9b9ff8c960f8b72301f2803ef Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Jul 2025 13:48:44 +0200
Subject: [PATCH 683/887] update snap

---
 tests/test_stub.nf.test.snap | 24 ++++++++++++------------
 1 file changed, 12 insertions(+), 12 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index e450622b3..fb5db47c0 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1214,8 +1214,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1460,7 +1460,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T11:42:59.015299"
+        "timestamp": "2025-07-22T13:42:49.769985"
     },
     "stub test fastp": {
         "content": [
@@ -1544,8 +1544,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1607,7 +1607,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T11:04:33.615515"
+        "timestamp": "2025-07-22T13:36:15.064231"
     },
     "stub test fusioncatcher trim": {
         "content": [
@@ -1620,8 +1620,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1689,7 +1689,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-17T10:05:52.553051"
+        "timestamp": "2025-07-22T13:37:38.01216"
     },
     "stub test all": {
         "content": [
@@ -1723,8 +1723,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -2178,6 +2178,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T15:39:15.996857"
+        "timestamp": "2025-07-22T13:28:04.670405"
     }
 }
\ No newline at end of file

From 000f3a33551c4b54c480bb15fbd6f69d8761718f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Jul 2025 14:15:06 +0200
Subject: [PATCH 684/887] update snaps

---
 tests/test_stub_bam.nf.test.snap | 18 +++++++++---------
 1 file changed, 9 insertions(+), 9 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 4a3dd8c85..0bfafe370 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -1107,8 +1107,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1326,7 +1326,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T13:08:14.629821"
+        "timestamp": "2025-07-22T14:08:17.97268"
     },
     "stub test fastp": {
         "content": [
@@ -1429,8 +1429,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1511,7 +1511,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T09:42:24.513478"
+        "timestamp": "2025-07-22T14:03:08.199969"
     },
     "stub test all": {
         "content": [
@@ -1545,8 +1545,8 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
+                    "gdown": "5.2.0",
+                    "tar": 1.34
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
@@ -1976,6 +1976,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T16:09:39.371336"
+        "timestamp": "2025-07-22T13:58:16.809449"
     }
 }
\ No newline at end of file

From 6c65018758c83953a08d1d961343847f5e6cff27 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Jul 2025 15:06:31 +0200
Subject: [PATCH 685/887] fix publish paths of build_references

---
 conf/modules.config | 22 +++++++++++-----------
 1 file changed, 11 insertions(+), 11 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index f7e712a93..f75af90b9 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -50,7 +50,7 @@ process {
 
     withName: 'GENCODE_DOWNLOAD' {
         publishDir = [
-            path: { "${params.genomes_base}/gencode" },
+            path: { Nextflow.file(params.fasta).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -125,7 +125,7 @@ process {
     withName: 'FUSIONREPORT_DOWNLOAD' {
         ext.args         = { params.no_cosmic ? "--no-cosmic" : params.qiagen ? "--qiagen --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" : "--cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         publishDir = [
-            path: { "${params.genomes_base}" },
+            path: { Nextflow.file(params.fusionreport_ref).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -133,7 +133,7 @@ process {
 
     withName: 'GATK4_BEDTOINTERVALLIST' {
         publishDir = [
-            path: { "${params.genomes_base}/gencode" },
+            path: { Nextflow.file(params.rrna_intervals).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -150,7 +150,7 @@ process {
     withName: 'GFFREAD' {
         ext.args = { '-w -S' }
         publishDir = [
-            path: { "${params.genomes_base}/gffread" },
+            path: { Nextflow.file(params.gtf).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -159,7 +159,7 @@ process {
     withName: 'GTF_TO_REFFLAT' {
         ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons"
         publishDir = [
-            path: { "${params.genomes_base}/gencode" },
+            path: { Nextflow.file(params.refflat).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -167,7 +167,7 @@ process {
 
     withName: 'HGNC_DOWNLOAD' {
         publishDir = [
-            path: { "${params.genomes_base}/hgnc" },
+            path: { Nextflow.file(params.hgnc_ref).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -208,7 +208,7 @@ process {
 
     withName: 'SALMON_INDEX' {
         publishDir = [
-            path: { "${params.genomes_base}/salmon" },
+            path: { Nextflow.file(params.salmon_index).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -228,7 +228,7 @@ process {
 
     withName: 'SAMTOOLS_FAIDX' {
         publishDir = [
-            path: { "${params.genomes_base}/gencode" },
+            path: { Nextflow.file(params.fai).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -289,7 +289,7 @@ process {
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }
         publishDir = [
-            path: { "${params.genomes_base}" },
+            path: { Nextflow.file(params.starindex_ref).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -301,7 +301,7 @@ process {
         memory = { 150.GB * task.attempt }
         time   = { 2.d  * task.attempt }
         publishDir = [
-            path: { "${params.genomes_base}/starfusion" },
+            path: { Nextflow.file(params.starfusion_ref).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -311,7 +311,7 @@ process {
         cpus   = { 1    * task.attempt }
         memory = { 20.GB * task.attempt }
         publishDir = [
-            path: { "${params.genomes_base}/starfusion" },
+            path: { Nextflow.file(params.starfusion_ref).parent },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]

From 842990c540f13c6574fc937cd57d7069e6784436 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 22 Jul 2025 13:31:05 +0000
Subject: [PATCH 686/887] refact: remove unused subworkflows

---
 CHANGELOG.md                                  |  1 +
 .../local/ctatsplicing_workflow/main.nf       | 28 --------
 .../local/fusionreport_workflow/main.nf       | 35 ---------
 .../fusionreport_workflow/tests/main.nf.test  | 71 -------------------
 .../tests/main.nf.test.snap                   | 46 ------------
 workflows/rnafusion.nf                        | 27 ++++---
 6 files changed, 17 insertions(+), 191 deletions(-)
 delete mode 100644 subworkflows/local/ctatsplicing_workflow/main.nf
 delete mode 100644 subworkflows/local/fusionreport_workflow/main.nf
 delete mode 100644 subworkflows/local/fusionreport_workflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 752d565a2..f8a1370e0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -116,6 +116,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed subworkflow with less than two modules: `ARRIBA_WORKFLOW` [#692](https://github.com/nf-core/rnafusion/pull/692)
 - Removed subworkflow with less than two modules: `STARFUSION_WORKFLOW` [#707](https://github.com/nf-core/rnafusion/pull/707)
 - Removed subworkflow with less than two modules: `CTATSPLICING_WORKFLOW` [#704](https://github.com/nf-core/rnafusion/pull/704)
+- Removed subworkflow with less than two modules: `FUSIONREPORT_WORKFLOW` [#721](https://github.com/nf-core/rnafusion/pull/721)
 
 ### Parameters
 
diff --git a/subworkflows/local/ctatsplicing_workflow/main.nf b/subworkflows/local/ctatsplicing_workflow/main.nf
deleted file mode 100644
index 02135973e..000000000
--- a/subworkflows/local/ctatsplicing_workflow/main.nf
+++ /dev/null
@@ -1,28 +0,0 @@
-include { CTATSPLICING_STARTOCANCERINTRONS } from '../../../modules/local/ctatsplicing/startocancerintrons'
-
-workflow CTATSPLICING_WORKFLOW {
-    take:
-    splice_junctions // [ val(meta), path(splice_junctions.SJ.out.tab) ]
-    junctions       // [ val(meta), path(junctions.Chimeric.out.junction) ]
-    aligned_bams    // [ val(meta), path(aligned_bams.Aligned.sortedByCoord.out.bam), path(aligned_bams.Aligned.sortedByCoord.out.bam.bai) ]
-    ctat_genome_lib // [ val(meta2), path(path/to/ctat_genome_lib) ]
-
-    main:
-    def ch_versions = Channel.empty()
-
-    def ch_ctatsplicing_input = splice_junctions
-        .join(junctions, failOnMismatch:true, failOnDuplicate:true)
-        .join(aligned_bams, failOnMismatch:true, failOnDuplicate:true)
-        .map { meta, split_junction, junction, bam, bai ->
-            [ meta, split_junction, junction, bam, bai ]
-        }
-
-    CTATSPLICING_STARTOCANCERINTRONS(
-        ch_ctatsplicing_input,
-        ctat_genome_lib
-    )
-    ch_versions = ch_versions.mix(CTATSPLICING_STARTOCANCERINTRONS.out.versions.first())
-
-    emit:
-    versions              = ch_versions
-}
diff --git a/subworkflows/local/fusionreport_workflow/main.nf b/subworkflows/local/fusionreport_workflow/main.nf
deleted file mode 100644
index 1235d724f..000000000
--- a/subworkflows/local/fusionreport_workflow/main.nf
+++ /dev/null
@@ -1,35 +0,0 @@
-include { FUSIONREPORT_DETECT      }     from '../../../modules/nf-core/fusionreport/detect/main'
-
-
-workflow FUSIONREPORT_WORKFLOW {
-    take:
-        fusionreport_ref
-        arriba_fusions
-        starfusion_fusions
-        fusioncatcher_fusions
-
-    main:
-        ch_versions = Channel.empty()
-        ch_report = Channel.empty()
-        ch_csv = Channel.empty()
-
-        def ch_fusions = arriba_fusions
-            .join(starfusion_fusions, failOnMismatch:true, failOnDuplicate:true)
-            .join(fusioncatcher_fusions, failOnMismatch:true, failOnDuplicate:true)
-
-        FUSIONREPORT_DETECT(ch_fusions, fusionreport_ref, params.tools_cutoff)
-        ch_fusion_list = FUSIONREPORT_DETECT.out.fusion_list
-        ch_fusion_list_filtered = FUSIONREPORT_DETECT.out.fusion_list_filtered
-        ch_versions = ch_versions.mix(FUSIONREPORT_DETECT.out.versions)
-        ch_report = FUSIONREPORT_DETECT.out.report
-        ch_csv = FUSIONREPORT_DETECT.out.csv
-
-    emit:
-        versions                 = ch_versions
-        fusion_list              = ch_fusion_list
-        fusion_list_filtered     = ch_fusion_list_filtered
-        report                   = ch_report
-        csv                      = ch_csv
-
-}
-
diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test b/subworkflows/local/fusionreport_workflow/tests/main.nf.test
deleted file mode 100644
index 63e44f1f8..000000000
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test
+++ /dev/null
@@ -1,71 +0,0 @@
-nextflow_workflow {
-
-    name "Test Subworkflow FUSIONREPORT_WORKFLOW"
-    script "../main.nf"
-    workflow "FUSIONREPORT_WORKFLOW"
-
-    tag "subworkflow"
-    tag "fusionreport_workflow"
-    tag "fusionreport"
-
-    test("FUSIONREPORT_WORKFLOW - Full Test") {
-
-        setup {
-            run("FUSIONREPORT_DOWNLOAD") {
-                script "../../../../modules/nf-core/fusionreport/download/main.nf"
-                process {
-                    """
-                    """
-                }
-            }
-        }
-
-        when {
-            workflow {
-                """
-                input[0] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
-
-                input[1] = Channel.of(
-                    [[ id:'test_sample' ],
-                        file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/arriba.tsv") ]
-                )
-
-                input[2] =  Channel.of(
-                    [[ id:'test_sample' ],
-                        file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/starfusion.tsv") ]
-                )
-
-                input[3] =  Channel.of(
-                    [[ id:'test_sample' ],
-                        file("https://github.com/Clinical-Genomics/fusion-report/raw/master/tests/test_data/fusioncatcher.txt") ]
-                )
-
-                """
-            }
-            params {
-                fusioninspector_only = false
-                tools_cutoff    = 1
-                arriba          = true
-                starfusion      = true
-                fusioncatcher   = true
-                no_cosmic       = true
-                outdir          = "$outputDir"
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(
-                    workflow.out.fusion_list,
-                    workflow.out.fusion_list_filtered,
-                    workflow.out.report,
-                    workflow.out.csv,
-                    workflow.out.versions
-                ).match() }
-            )
-        }
-    }
-
-
-}
diff --git a/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap b/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 4dd686d8a..000000000
--- a/subworkflows/local/fusionreport_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,46 +0,0 @@
-{
-    "FUSIONREPORT_WORKFLOW - Full Test": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test_sample"
-                    },
-                    "test_sample.fusionreport.tsv:md5,3593b7021f26cc5427fdc96f0d1c72f0"
-                ]
-            ],
-            [
-                [
-                    {
-                        "id": "test_sample"
-                    },
-                    "test_sample.fusionreport_filtered.tsv:md5,3593b7021f26cc5427fdc96f0d1c72f0"
-                ]
-            ],
-            [
-                [
-                    {
-                        "id": "test_sample"
-                    },
-                    "test_sample_fusionreport_index.html:md5,9dfbf94471173bebbd8085e440515e4d"
-                ]
-            ],
-            [
-                [
-                    {
-                        "id": "test_sample"
-                    },
-                    "test_sample.fusions.csv:md5,ee6c6838110d68bc75a13c19dca0a321"
-                ]
-            ],
-            [
-                "versions.yml:md5,5b80da331704f6d2093b7609537222e8"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-19T11:32:39.322725"
-    }
-}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 0d4666aed..83aa4d52c 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -12,7 +12,7 @@ include { STARFUSION                    }   from '../modules/local/starfusion/de
 include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
 include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
-include { FUSIONREPORT_WORKFLOW         }   from '../subworkflows/local/fusionreport_workflow'
+include { FUSIONREPORT_DETECT           }   from '../modules/nf-core/fusionreport/detect/main'
 include { FASTQC                        }   from '../modules/nf-core/fastqc/main'
 include { MULTIQC                       }   from '../modules/nf-core/multiqc/main'
 include { STAR_ALIGN                    }   from '../modules/nf-core/star/align/main'
@@ -308,21 +308,26 @@ workflow RNAFUSION {
         def ch_fusion_list_filtered = Channel.empty()
         def ch_fusionreport_report = Channel.empty()
         def ch_fusionreport_csv = Channel.empty()
-        if(!params.skip_vis && tools.contains("fusionreport")) {
+        if (!params.skip_vis && tools.contains("fusionreport")) {
             if (!fusions_created) {
                 error("Could not find any fusion files. Please generate some with --arriba, --starfusion and/or --fusioncatcher")
             }
-            FUSIONREPORT_WORKFLOW (
+
+            def ch_fusions = ch_arriba_fusions
+                .join(ch_starfusion_fusions, failOnMismatch:true, failOnDuplicate:true)
+                .join(ch_fusioncatcher_fusions, failOnMismatch:true, failOnDuplicate:true)
+
+            FUSIONREPORT_DETECT(
+                ch_fusions,
                 BUILD_REFERENCES.out.fusionreport_ref,
-                ch_arriba_fusions,
-                ch_starfusion_fusions,
-                ch_fusioncatcher_fusions
+                params.tools_cutoff
             )
-            ch_versions             = ch_versions.mix(FUSIONREPORT_WORKFLOW.out.versions)
-            ch_fusion_list          = FUSIONREPORT_WORKFLOW.out.fusion_list
-            ch_fusion_list_filtered = FUSIONREPORT_WORKFLOW.out.fusion_list_filtered
-            ch_fusionreport_report  = FUSIONREPORT_WORKFLOW.out.report
-            ch_fusionreport_csv     = FUSIONREPORT_WORKFLOW.out.csv
+
+            ch_versions             = ch_versions.mix(FUSIONREPORT_DETECT.out.versions)
+            ch_fusion_list          = FUSIONREPORT_DETECT.out.fusion_list
+            ch_fusion_list_filtered = FUSIONREPORT_DETECT.out.fusion_list_filtered
+            ch_fusionreport_report  = FUSIONREPORT_DETECT.out.report
+            ch_fusionreport_csv     = FUSIONREPORT_DETECT.out.csv
         } else if(params.fusioninspector_fusions) {
             def input_fusions       = file(params.fusioninspector_fusions, checkIfExists:true)
             ch_fusion_list          = ch_reads.map { it -> [ it[0], input_fusions ] }

From 8ee1d5a2149168797997339a207258861d98c99d Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Jul 2025 13:57:40 +0000
Subject: [PATCH 687/887] fix build_refs test

---
 conf/modules.config                         |  22 +-
 subworkflows/local/build_references/main.nf |  10 +-
 tests/test_build.nf.test                    |   8 +-
 tests/test_build.nf.test.snap               | 306 +++++++++++---------
 4 files changed, 192 insertions(+), 154 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index f75af90b9..d56ddb33f 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -50,7 +50,7 @@ process {
 
     withName: 'GENCODE_DOWNLOAD' {
         publishDir = [
-            path: { Nextflow.file(params.fasta).parent },
+            path: { "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -125,7 +125,7 @@ process {
     withName: 'FUSIONREPORT_DOWNLOAD' {
         ext.args         = { params.no_cosmic ? "--no-cosmic" : params.qiagen ? "--qiagen --cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" : "--cosmic_usr ${params.cosmic_username} --cosmic_passwd ${params.cosmic_passwd}" }
         publishDir = [
-            path: { Nextflow.file(params.fusionreport_ref).parent },
+            path: { "${params.genomes_base}/GRCh38" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -133,7 +133,7 @@ process {
 
     withName: 'GATK4_BEDTOINTERVALLIST' {
         publishDir = [
-            path: { Nextflow.file(params.rrna_intervals).parent },
+            path: {  "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
@@ -150,7 +150,7 @@ process {
     withName: 'GFFREAD' {
         ext.args = { '-w -S' }
         publishDir = [
-            path: { Nextflow.file(params.gtf).parent },
+            path: {  "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -159,7 +159,7 @@ process {
     withName: 'GTF_TO_REFFLAT' {
         ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons"
         publishDir = [
-            path: { Nextflow.file(params.refflat).parent },
+            path: {  "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -167,7 +167,7 @@ process {
 
     withName: 'HGNC_DOWNLOAD' {
         publishDir = [
-            path: { Nextflow.file(params.hgnc_ref).parent },
+            path: { "${params.genomes_base}/GRCh38/hgnc" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -208,7 +208,7 @@ process {
 
     withName: 'SALMON_INDEX' {
         publishDir = [
-            path: { Nextflow.file(params.salmon_index).parent },
+            path: { "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -228,7 +228,7 @@ process {
 
     withName: 'SAMTOOLS_FAIDX' {
         publishDir = [
-            path: { Nextflow.file(params.fai).parent },
+            path: { "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -289,7 +289,7 @@ process {
         memory = { 100.GB * task.attempt }
         time   = { 2.d  * task.attempt }
         publishDir = [
-            path: { Nextflow.file(params.starindex_ref).parent },
+            path: { "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -301,7 +301,7 @@ process {
         memory = { 150.GB * task.attempt }
         time   = { 2.d  * task.attempt }
         publishDir = [
-            path: { Nextflow.file(params.starfusion_ref).parent },
+            path: { "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/starfusion" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
@@ -311,7 +311,7 @@ process {
         cpus   = { 1    * task.attempt }
         memory = { 20.GB * task.attempt }
         publishDir = [
-            path: { Nextflow.file(params.starfusion_ref).parent },
+            path: { "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/starfusion" },
             mode: params.publish_dir_mode,
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename },
         ]
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 77ef7c331..762aebd5b 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -131,12 +131,12 @@ workflow BUILD_REFERENCES {
         }
     }
 
-    def ch_arriba_ref_blacklist       = Channel.fromPath(params.arriba_ref_blacklist)
-    def ch_arriba_ref_cytobands       = Channel.fromPath(params.arriba_ref_cytobands)
-    def ch_arriba_ref_known_fusions   = Channel.fromPath(params.arriba_ref_known_fusions)
-    def ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains)
+    def ch_arriba_ref_blacklist       = params.arriba_ref_blacklist ? Channel.fromPath(params.arriba_ref_blacklist) : Channel.empty()
+    def ch_arriba_ref_cytobands       = params.arriba_ref_cytobands ? Channel.fromPath(params.arriba_ref_cytobands) : Channel.empty()
+    def ch_arriba_ref_known_fusions   = params.arriba_ref_known_fusions ? Channel.fromPath(params.arriba_ref_known_fusions) : Channel.empty()
+    def ch_arriba_ref_protein_domains = params.arriba_ref_protein_domains ? Channel.fromPath(params.arriba_ref_protein_domains) : Channel.empty()
 
-    def ch_fusioncatcher_ref = Channel.fromPath(params.fusioncatcher_ref).map { it -> [[id:it.name], it] }
+    def ch_fusioncatcher_ref = params.fusioncatcher_ref ? Channel.fromPath(params.fusioncatcher_ref).map { it -> [[id:it.name], it] } : Channel.empty()
 
     def ch_starfusion_ref = Channel.empty()
     if (tools.intersect(["starfusion", "ctatsplicing", "fusioninspector"])) {
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 4157bffea..9a75dcce5 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -18,8 +18,14 @@ nextflow_pipeline {
                 // Input data
                 references_only            = true
                 input                      = "${projectDir}/tests/csv/fastq.csv"
+                genomes_base               = "${outputDir}/references"
+                fusioncatcher_ref          = null
+                arriba_ref_protein_domains = null
+                arriba_ref_known_fusions   = null
+                arriba_ref_blacklist       = null
+                arriba_ref_cytobands       = null
                 no_cosmic                  = true
-                tools                      = "arriba,starfusion,fusionreport,salmon,fusioninspector"
+                tools                      = "all"
                 fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'
                 gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
                 fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index e557a7a15..8c55297bd 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -3,8 +3,8 @@
         "content": [
             12,
             {
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
+                "CTATSPLICING_PREPGENOMELIB": {
+                    "ctat-splicing": "0.0.2"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "4.0.1"
@@ -55,160 +55,167 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/gencode",
-                "references/gencode/minigenome.fa.fai",
-                "references/gencode/minigenome.gtf.interval_list",
-                "references/gencode/minigenome.gtf.refflat",
-                "references/gffread",
-                "references/gffread/minigenome.gtf.fasta",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38/hgnc",
+                "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
+                "references/GRCh38/hgnc/hgnc_complete_set.txt"
             ],
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-                "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "minigenome.gtf.fasta:md5,d41d8cd98f00b204e9800998ecf8427e",
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-                "minigenome.gtf.fasta:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "complete_ref_lens.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
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                 "ctg_offsets.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
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                 "info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "mphf.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "pos.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
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                 "refAccumLengths.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_indexing.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "reflengths.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "refseq.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "seq.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "versionInfo.json:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
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                 "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -218,11 +225,13 @@
                 "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -246,20 +255,39 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
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+                "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cancer_splicing.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "refGene.sort.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "refGene.sort.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -270,18 +298,22 @@
                 "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "HGNC-DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T15:25:05.434296"
+        "timestamp": "2025-07-22T13:55:10.011645035"
     }
 }
\ No newline at end of file

From 4baff3a3f3f09f76fae4022ba02f04dadbf55d02 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Jul 2025 16:28:41 +0200
Subject: [PATCH 688/887] update snap

---
 .../starfusion/detect/tests/main.nf.test.snap    | 16 ++++++++--------
 1 file changed, 8 insertions(+), 8 deletions(-)

diff --git a/modules/local/starfusion/detect/tests/main.nf.test.snap b/modules/local/starfusion/detect/tests/main.nf.test.snap
index f65f94b02..55f6c6fee 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test.snap
+++ b/modules/local/starfusion/detect/tests/main.nf.test.snap
@@ -30,7 +30,7 @@
                     ]
                 ],
                 "3": [
-                    "versions.yml:md5,a8fb6344fdf740dde0941048313fc243"
+                    "versions.yml:md5,1597cbbd06bf7278faa9c6282665cbac"
                 ],
                 "abridged": [
                     [
@@ -60,15 +60,15 @@
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,a8fb6344fdf740dde0941048313fc243"
+                    "versions.yml:md5,1597cbbd06bf7278faa9c6282665cbac"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-12-19T18:42:06.988178092"
+        "timestamp": "2025-07-22T16:26:57.806399"
     },
     "Should run without failures": {
         "content": [
@@ -96,17 +96,17 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.starfusion.abridged.coding_effect.tsv:md5,95dfce6fdaf3589f23881fe1e855c62b"
+                    "test.starfusion.abridged.coding_effect.tsv:md5,760ef75cd5d17fd0adf3733d215fbca6"
                 ]
             ],
             [
-                "versions.yml:md5,a8fb6344fdf740dde0941048313fc243"
+                "versions.yml:md5,1597cbbd06bf7278faa9c6282665cbac"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.3"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-12-19T18:41:49.150362156"
+        "timestamp": "2025-07-22T16:26:23.317406"
     }
 }
\ No newline at end of file

From 1cb3453e0b80ebde24b51fa8e8f07459cc45fbe4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Jul 2025 16:30:16 +0200
Subject: [PATCH 689/887] make sure tests don't use the S3 refs

---
 tests/nextflow.config       |  8 ++++++++
 tests/test_cosmic.nf.test   |  1 +
 tests/test_stub.nf.test     | 16 +++++++++++++++-
 tests/test_stub_bam.nf.test | 10 ++++++++++
 4 files changed, 34 insertions(+), 1 deletion(-)

diff --git a/tests/nextflow.config b/tests/nextflow.config
index b1db55ff5..cefc7f7bf 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -10,3 +10,11 @@ process {
         ext.args = ''
     }
 }
+
+params {
+    fusioncatcher_ref           = "${projectDir}/assets"
+    arriba_ref_blacklist        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_cytobands        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/cytobands_hg38_GRCh38_v2.5.0.tsv"
+    arriba_ref_known_fusions    = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_protein_domains  = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+}
diff --git a/tests/test_cosmic.nf.test b/tests/test_cosmic.nf.test
index b547b2fe4..f5c345be1 100644
--- a/tests/test_cosmic.nf.test
+++ b/tests/test_cosmic.nf.test
@@ -13,6 +13,7 @@ nextflow_pipeline {
             params {
                 outdir               = "$outputDir"
                 tools                = "fastp"
+                genomes_base         = "${outputDir}/references"
             }
         }
 
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index 495d20b3d..496eda104 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -15,7 +15,9 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 tools            = "all"
-                no_cosmic              = true
+                no_cosmic        = true
+                genomes_base     = "${outputDir}/references"
+
             }
         }
 
@@ -48,6 +50,8 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "arriba"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
+
             }
         }
 
@@ -80,6 +84,8 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "ctatsplicing"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
+
             }
         }
 
@@ -112,6 +118,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "fusioncatcher"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -145,6 +152,7 @@ nextflow_pipeline {
                 tools            = "fusioncatcher"
                 trim_tail_fusioncatcher = 50
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
 
             }
         }
@@ -177,6 +185,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "starfusion"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -209,6 +218,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "stringtie"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -242,6 +252,7 @@ nextflow_pipeline {
                 tools            = "fusionreport,arriba,starfusion,fusioncatcher"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 no_cosmic        = true
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -274,6 +285,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "fastp"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -306,6 +318,7 @@ nextflow_pipeline {
                 outdir           = "$outputDir"
                 tools            = "salmon"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -339,6 +352,7 @@ nextflow_pipeline {
                 tools            = "fusioninspector,arriba,fusionreport"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 no_cosmic        = true
+                genomes_base     = "${outputDir}/references"
             }
         }
 
diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
index cea94105e..25d4370c6 100644
--- a/tests/test_stub_bam.nf.test
+++ b/tests/test_stub_bam.nf.test
@@ -17,6 +17,7 @@ nextflow_pipeline {
                 input            = "${projectDir}/tests/yml/bam.yml"
                 tools            = "all"
                 no_cosmic        = true
+                genomes_base     = "${outputDir}/references"
 
             }
         }
@@ -52,6 +53,7 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 no_cosmic        = true
+                genomes_base     = "${outputDir}/references"
 
             }
         }
@@ -86,6 +88,7 @@ nextflow_pipeline {
                 tools            = "ctatsplicing"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -119,6 +122,7 @@ nextflow_pipeline {
                 tools            = "fusioncatcher"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -152,6 +156,7 @@ nextflow_pipeline {
                 tools            = "starfusion"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -185,6 +190,7 @@ nextflow_pipeline {
                 tools            = "stringtie"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -219,6 +225,7 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 no_cosmic        = true
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -252,6 +259,7 @@ nextflow_pipeline {
                 tools            = "fastp"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -285,6 +293,7 @@ nextflow_pipeline {
                 tools            = "salmon"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                genomes_base     = "${outputDir}/references"
             }
         }
 
@@ -319,6 +328,7 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 no_cosmic        = true
+                genomes_base     = "${outputDir}/references"
             }
         }
 

From 261d9f44fe56f02a24daf91a1c5371ed89cb8b7c Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Jul 2025 15:02:52 +0000
Subject: [PATCH 690/887] fix test_stub

---
 tests/test_stub.nf.test.snap | 1398 +++++++++++++++++-----------------
 1 file changed, 678 insertions(+), 720 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 181adc772..9dccfa104 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -83,105 +83,107 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -209,9 +211,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-16T09:22:13.085521"
+        "timestamp": "2025-07-22T14:57:52.257731378"
     },
     "stub test stringtie": {
         "content": [
@@ -296,29 +298,31 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -349,20 +353,17 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-16T09:24:22.233178"
+        "timestamp": "2025-07-22T14:58:41.747600647"
     },
     "stub test arriba": {
         "content": [
-            16,
+            15,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -439,34 +440,31 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -492,11 +490,11 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T11:31:30.841349"
+        "timestamp": "2025-07-22T14:54:33.526664584"
     },
     "stub test fusioninspector": {
         "content": [
-            24,
+            23,
             {
                 "AGAT_CONVERTSPGFF2TSV": {
                     "agat": "v1.4.1"
@@ -504,9 +502,6 @@
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
                 },
@@ -737,119 +732,116 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
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-                "references/starfusion",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38",
+                "references/GRCh38/fusion_report_db",
+                "references/GRCh38/fusion_report_db/DB-timestamp.txt",
+                "references/GRCh38/fusion_report_db/cosmic.db",
+                "references/GRCh38/fusion_report_db/fusion_report.log",
+                "references/GRCh38/fusion_report_db/fusiongdb2.db",
+                "references/GRCh38/fusion_report_db/mitelman.db",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38/hgnc",
+                "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
+                "references/GRCh38/hgnc/hgnc_complete_set.txt",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -877,7 +869,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T16:00:28.831228"
+        "timestamp": "2025-07-22T15:02:29.884427377"
     },
     "stub test salmon": {
         "content": [
@@ -933,31 +925,31 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/salmon",
+                "references/GRCh38/gencode_v46/salmon/complete_ref_lens.bin",
+                "references/GRCh38/gencode_v46/salmon/ctable.bin",
+                "references/GRCh38/gencode_v46/salmon/ctg_offsets.bin",
+                "references/GRCh38/gencode_v46/salmon/duplicate_clusters.tsv",
+                "references/GRCh38/gencode_v46/salmon/info.json",
+                "references/GRCh38/gencode_v46/salmon/mphf.bin",
+                "references/GRCh38/gencode_v46/salmon/pos.bin",
+                "references/GRCh38/gencode_v46/salmon/pre_indexing.log",
+                "references/GRCh38/gencode_v46/salmon/rank.bin",
+                "references/GRCh38/gencode_v46/salmon/refAccumLengths.bin",
+                "references/GRCh38/gencode_v46/salmon/ref_indexing.log",
+                "references/GRCh38/gencode_v46/salmon/reflengths.bin",
+                "references/GRCh38/gencode_v46/salmon/refseq.bin",
+                "references/GRCh38/gencode_v46/salmon/seq.bin",
+                "references/GRCh38/gencode_v46/salmon/versionInfo.json",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -966,9 +958,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-16T09:27:23.823019"
+        "timestamp": "2025-07-22T15:01:29.71875007"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -1069,110 +1061,112 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
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-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
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+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.bed",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -1196,27 +1190,20 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-13T19:05:06.954764"
+        "timestamp": "2025-07-22T14:55:32.804299315"
     },
     "stub test fusionreport": {
         "content": [
-            22,
+            20,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
-                },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
@@ -1318,119 +1305,113 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38",
+                "references/GRCh38/fusion_report_db",
+                "references/GRCh38/fusion_report_db/DB-timestamp.txt",
+                "references/GRCh38/fusion_report_db/cosmic.db",
+                "references/GRCh38/fusion_report_db/fusion_report.log",
+                "references/GRCh38/fusion_report_db/fusiongdb2.db",
+                "references/GRCh38/fusion_report_db/mitelman.db",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -1460,7 +1441,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T11:42:59.015299"
+        "timestamp": "2025-07-22T14:59:37.673773299"
     },
     "stub test fastp": {
         "content": [
@@ -1522,31 +1503,29 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-16T09:26:06.949386"
+        "timestamp": "2025-07-22T15:00:31.452519757"
     },
     "stub test fusioncatcher": {
         "content": [
-            10,
+            9,
             {
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
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-                    "tar": 1.35
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                 "FUSIONCATCHER_FUSIONCATCHER": {
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@@ -1592,26 +1571,25 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
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         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T11:04:33.615515"
+        "timestamp": "2025-07-22T14:56:09.844925286"
     },
     "stub test fusioncatcher trim": {
         "content": [
-            11,
+            10,
             {
                 "FASTP_FOR_FUSIONCATCHER": {
                     "fastp": "0.24.0"
@@ -1619,10 +1597,6 @@
                 "FASTQC": {
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                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
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@@ -1674,26 +1648,25 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
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         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
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-        "timestamp": "2025-06-19T11:19:49.213204"
+        "timestamp": "2025-07-22T14:57:01.736743773"
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     "stub test all": {
         "content": [
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+            34,
             {
                 "AGAT_CONVERTSPGFF2TSV": {
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@@ -1701,9 +1674,6 @@
                 "ARRIBA_ARRIBA": {
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-                    "arriba_download": "2.5.0"
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                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
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@@ -1722,10 +1692,6 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
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-                    "tar": 1.35
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                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
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@@ -1996,146 +1962,138 @@
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-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
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-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
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-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
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-                "references/gffread",
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-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
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-                "references/salmon/salmon",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38/hgnc",
+                "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
+                "references/GRCh38/hgnc/hgnc_complete_set.txt",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -2178,6 +2136,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T15:39:15.996857"
+        "timestamp": "2025-07-22T14:53:33.739317046"
     }
 }
\ No newline at end of file

From a03df57ab3e7d5ee3e4823416f4774e7a5e9e1bf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Jul 2025 17:14:11 +0200
Subject: [PATCH 691/887] update snap

---
 modules/local/fusioninspector/tests/main.nf.test.snap | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 44a554689..42ecbe8c4 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -398,7 +398,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.tsv:md5,01a2578b59f112d23a1475cd75b9af0b"
+                    "test.FusionInspector.fusions.tsv:md5,c992c4485f060b1c41be5c29efc4abba"
                 ]
             ],
             [
@@ -416,7 +416,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.abridged.tsv:md5,98d99b567527d990086689e4f7f8cc12"
+                    "test.FusionInspector.fusions.abridged.tsv:md5,727aed24358441c63fe08b0e6aaca42b"
                 ]
             ],
             [
@@ -425,14 +425,14 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "FusionInspector.log:md5,6cdafdbfe281ca9bb92c484e5a001d41"
+                    "FusionInspector.log:md5,fc708e06807eb0c84a182a11b1c5112c"
                 ]
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-30T15:11:10.528898"
+        "timestamp": "2025-07-22T17:01:54.346734"
     }
 }
\ No newline at end of file

From 4a9ef207abdac69c0263e4934dd379cb9de29dd1 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 22 Jul 2025 15:17:27 +0000
Subject: [PATCH 692/887] fix test_stub_bam tests

---
 tests/test_stub_bam.nf.test.snap | 1365 +++++++++++++++---------------
 1 file changed, 664 insertions(+), 701 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 4a3dd8c85..a07c1e0cd 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -75,105 +75,107 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "starfusion",
                 "starfusion/test.starfusion.abridged.coding_effect.tsv",
                 "starfusion/test.starfusion.abridged.tsv",
@@ -184,7 +186,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T10:14:43.131367"
+        "timestamp": "2025-07-22T15:09:47.135186171"
     },
     "stub test stringtie": {
         "content": [
@@ -261,29 +263,31 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -300,18 +304,15 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-02T17:05:41.044302"
+        "timestamp": "2025-07-22T15:10:57.606042435"
     },
     "stub test arriba": {
         "content": [
-            14,
+            13,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -380,45 +381,42 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab"
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T12:57:24.512309"
+        "timestamp": "2025-07-22T15:06:09.138433288"
     },
     "stub test fusioninspector": {
         "content": [
-            23,
+            22,
             {
                 "AGAT_CONVERTSPGFF2TSV": {
                     "agat": "v1.4.1"
@@ -426,9 +424,6 @@
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
                 },
@@ -651,119 +646,116 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38",
+                "references/GRCh38/fusion_report_db",
+                "references/GRCh38/fusion_report_db/DB-timestamp.txt",
+                "references/GRCh38/fusion_report_db/cosmic.db",
+                "references/GRCh38/fusion_report_db/fusion_report.log",
+                "references/GRCh38/fusion_report_db/fusiongdb2.db",
+                "references/GRCh38/fusion_report_db/mitelman.db",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38/hgnc",
+                "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
+                "references/GRCh38/hgnc/hgnc_complete_set.txt",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
@@ -775,7 +767,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T16:53:36.788101"
+        "timestamp": "2025-07-22T15:17:08.528288381"
     },
     "stub test salmon": {
         "content": [
@@ -850,31 +842,31 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/salmon",
+                "references/GRCh38/gencode_v46/salmon/complete_ref_lens.bin",
+                "references/GRCh38/gencode_v46/salmon/ctable.bin",
+                "references/GRCh38/gencode_v46/salmon/ctg_offsets.bin",
+                "references/GRCh38/gencode_v46/salmon/duplicate_clusters.tsv",
+                "references/GRCh38/gencode_v46/salmon/info.json",
+                "references/GRCh38/gencode_v46/salmon/mphf.bin",
+                "references/GRCh38/gencode_v46/salmon/pos.bin",
+                "references/GRCh38/gencode_v46/salmon/pre_indexing.log",
+                "references/GRCh38/gencode_v46/salmon/rank.bin",
+                "references/GRCh38/gencode_v46/salmon/refAccumLengths.bin",
+                "references/GRCh38/gencode_v46/salmon/ref_indexing.log",
+                "references/GRCh38/gencode_v46/salmon/reflengths.bin",
+                "references/GRCh38/gencode_v46/salmon/refseq.bin",
+                "references/GRCh38/gencode_v46/salmon/seq.bin",
+                "references/GRCh38/gencode_v46/salmon/versionInfo.json",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -885,7 +877,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T10:34:40.653967"
+        "timestamp": "2025-07-22T15:16:17.311147319"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -978,110 +970,112 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai"
@@ -1091,25 +1085,18 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T09:40:30.61019"
+        "timestamp": "2025-07-22T15:07:27.162452256"
     },
     "stub test fusionreport": {
         "content": [
-            20,
+            18,
             {
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
-                },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
@@ -1203,119 +1190,113 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38",
+                "references/GRCh38/fusion_report_db",
+                "references/GRCh38/fusion_report_db/DB-timestamp.txt",
+                "references/GRCh38/fusion_report_db/cosmic.db",
+                "references/GRCh38/fusion_report_db/fusion_report.log",
+                "references/GRCh38/fusion_report_db/fusiongdb2.db",
+                "references/GRCh38/fusion_report_db/mitelman.db",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "starfusion",
                 "starfusion/test.starfusion.abridged.coding_effect.tsv",
                 "starfusion/test.starfusion.abridged.tsv",
@@ -1326,7 +1307,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-08T13:08:14.629821"
+        "timestamp": "2025-07-22T15:14:13.492380412"
     },
     "stub test fastp": {
         "content": [
@@ -1407,31 +1388,29 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-05-07T13:29:36.326072757"
+        "timestamp": "2025-07-22T15:14:58.894874205"
     },
     "stub test fusioncatcher": {
         "content": [
-            13,
+            12,
             {
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
-                },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
@@ -1496,26 +1475,25 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-03T09:42:24.513478"
+        "timestamp": "2025-07-22T15:08:36.43147108"
     },
     "stub test all": {
         "content": [
-            35,
+            33,
             {
                 "AGAT_CONVERTSPGFF2TSV": {
                     "agat": "v1.4.1"
@@ -1523,9 +1501,6 @@
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
                 },
@@ -1544,10 +1519,6 @@
                 "FASTQC_FOR_FASTP": {
                     "fastqc": "0.12.1"
                 },
-                "FUSIONCATCHER_DOWNLOAD": {
-                    "wget": "1.21.4",
-                    "tar": 1.35
-                },
                 "FUSIONCATCHER_FUSIONCATCHER": {
                     "fusioncatcher": "1.33"
                 },
@@ -1810,146 +1781,138 @@
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
-                "references/arriba",
-                "references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv",
-                "references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz",
-                "references/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3",
-                "references/fusion_report_db",
-                "references/fusion_report_db/DB-timestamp.txt",
-                "references/fusion_report_db/cosmic.db",
-                "references/fusion_report_db/fusion_report.log",
-                "references/fusion_report_db/fusiongdb2.db",
-                "references/fusion_report_db/mitelman.db",
-                "references/fusioncatcher",
-                "references/fusioncatcher/human_v46",
-                "references/fusioncatcher/human_v46/ensembl_fully_overlapping_genes.txt",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/gffread",
-                "references/gffread/Homo_sapiens.GRCh38.46.gtf.fasta",
-                "references/hgnc",
-                "references/hgnc/HGNC-DB-timestamp.txt",
-                "references/hgnc/hgnc_complete_set.txt",
-                "references/salmon",
-                "references/salmon/salmon",
-                "references/salmon/salmon/complete_ref_lens.bin",
-                "references/salmon/salmon/ctable.bin",
-                "references/salmon/salmon/ctg_offsets.bin",
-                "references/salmon/salmon/duplicate_clusters.tsv",
-                "references/salmon/salmon/info.json",
-                "references/salmon/salmon/mphf.bin",
-                "references/salmon/salmon/pos.bin",
-                "references/salmon/salmon/pre_indexing.log",
-                "references/salmon/salmon/rank.bin",
-                "references/salmon/salmon/refAccumLengths.bin",
-                "references/salmon/salmon/ref_indexing.log",
-                "references/salmon/salmon/reflengths.bin",
-                "references/salmon/salmon/refseq.bin",
-                "references/salmon/salmon/seq.bin",
-                "references/salmon/salmon/versionInfo.json",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/ctat_genome_lib_build_dir",
-                "references/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
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-                "references/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
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-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
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-                "references/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
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+                "references/GRCh38/fusion_report_db",
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+                "references/GRCh38/fusion_report_db/cosmic.db",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38/hgnc",
+                "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
+                "references/GRCh38/hgnc/hgnc_complete_set.txt",
                 "salmon",
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
@@ -1976,6 +1939,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-14T16:09:39.371336"
+        "timestamp": "2025-07-22T15:04:39.683600348"
     }
 }
\ No newline at end of file

From 0236e2939d11c8d9cea295ef207c17cf87a192fb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 22 Jul 2025 17:29:36 +0200
Subject: [PATCH 693/887] update rocrate

---
 ro-crate-metadata.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 7b0e6e57b..b865b7385 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### Build references\n\n`--references_only` triggers a workflow to ONLY build references, otherwise the references are build when the analysis is run:\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### Build references\n\n`--references_only` triggers a workflow to ONLY build references, otherwise the references are build when the analysis is run:\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"

From f2b9c5d34d0facda0998970d95a2632e0a9cbfd4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 23 Jul 2025 09:11:34 +0200
Subject: [PATCH 694/887] update snap

---
 .../prepgenomelib/tests/main.nf.test.snap     | 42 +++++++++----------
 1 file changed, 21 insertions(+), 21 deletions(-)

diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
index ed1df4107..9aff241a2 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
@@ -9,7 +9,7 @@
                         },
                         [
                             "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-                            "PFAM.domtblout.dat.gz:md5,b8cf97310b4af1275237febab723d16c",
+                            "PFAM.domtblout.dat.gz:md5,075c489b35a7fd55b741102769af210f",
                             [
                                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -35,42 +35,42 @@
                                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
                             ],
-                            "blast_pairs.dat.gz:md5,31ec48d5116f44b12fdd3f47155241dc",
-                            "blast_pairs.idx:md5,0264ed1a28c50370e598cc8bd874dce0",
+                            "blast_pairs.dat.gz:md5,79b51d2021d564aba2bc931d52816114",
+                            "blast_pairs.idx:md5,c2d738b2541366d75d21b3503e5d5121",
                             [
-                                "cancer_splicing.idx:md5,fcec006060ebd0bfb1b0cf38c9f78116"
+                                "cancer_splicing.idx:md5,ca755ab82af1285bfbf945d92a260a6f"
                             ],
                             "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                            "fusion_annot_lib.idx:md5,21bc9b75123be07089e86023285b67f8",
-                            "pfam_domains.dbm:md5,68a00826b188aa577237a701e6f18e8c",
-                            "refGene.bed:md5,a14d9396c44c5fc0cd6b4d3194e8902d",
+                            "fusion_annot_lib.idx:md5,252532308d08ee6c012c69883c343d02",
+                            "pfam_domains.dbm:md5,a5c6c00f2f0759f85bdef39629b3e5d2",
+                            "refGene.bed:md5,2262b9ee22ea50009f4a8c51c6b2fac7",
                             "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
                             "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
-                            "ref_annot.cdna.fa:md5,4907484edddb1a52d1ef5acb814fa709",
-                            "ref_annot.cdna.fa.idx:md5,8f5c6a6c2ffec0188fac2483f806aabc",
-                            "ref_annot.cds:md5,de204070512c909b351c5876133f4f54",
-                            "ref_annot.cdsplus.fa:md5,180f4e5d277f2d5fc4bb092c11eaaf68",
-                            "ref_annot.cdsplus.fa.idx:md5,be8bb33932b6c2f82213a0622241ad0f",
+                            "ref_annot.cdna.fa:md5,b087fb1b388d75a88887bb98d8c5d339",
+                            "ref_annot.cdna.fa.idx:md5,d6c43237e5c522278f944edc79b5c567",
+                            "ref_annot.cds:md5,3686879a92b12a36ee89fcb4dfc9d9f8",
+                            "ref_annot.cdsplus.fa:md5,72561309cba7f598c641c19df84e9c0e",
+                            "ref_annot.cdsplus.fa.idx:md5,5751b6d5373f24fd98a2ce86618e3b7c",
                             "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                            "ref_annot.gtf.gene_spans:md5,4884ab6d453096b48bf85595299d9183",
+                            "ref_annot.gtf.gene_spans:md5,96105e687b1c346c773abc5915d817b2",
                             "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                             "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                            "ref_annot.pep:md5,22f05c0bf182e2645f0a01c4fb45e908",
-                            "ref_annot.prot_info.dbm:md5,218a49ac07f0db5fba0b1b48e6b12343",
+                            "ref_annot.pep:md5,28d1d869dcd616da362ead8ae39df21d",
+                            "ref_annot.prot_info.dbm:md5,18abe5ec2717d4c214d7546b998dfe44",
                             "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                             "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                             "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                             "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                             "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                            "ref_genome.fa.nin:md5,3c4114ca4fb5dd0dc2095f4cf5f7b844",
-                            "ref_genome.fa.njs:md5,39b423551f658bb0cb5032ac00bd74eb",
+                            "ref_genome.fa.nin:md5,d4d9344b71466f067e9a3d8043feb891",
+                            "ref_genome.fa.njs:md5,2b049589cf2e3ab830883dda45ffdf3c",
                             "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                             "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                             "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                             "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                             [
                                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                                "Log.out:md5,5310b905153fa779043520ec3fc7ca3b",
+                                "Log.out:md5,a352597c0bed7b2ae1018532a5893203",
                                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -81,14 +81,14 @@
                                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                                "genomeParameters.txt:md5,9664cf5658a941c5d4cf9f5bb5e5cb4f",
+                                "genomeParameters.txt:md5,09b8b0bb918b2c628030d9fb1aa7aea6",
                                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2"
                             ],
                             "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                            "trans.blast.align_coords.align_coords.dbm:md5,4ae2e5e8d141bd2e066da74ffb60403b",
+                            "trans.blast.align_coords.align_coords.dbm:md5,776d70b98b4be1290e3e187b5fef3a80",
                             "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
                         ]
                     ]
@@ -102,7 +102,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-16T09:41:16.195052"
+        "timestamp": "2025-07-23T08:54:58.892241"
     },
     "test - stub": {
         "content": [

From 40386ad65a58132ca0127cdd2273de26b9a9ef64 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 23 Jul 2025 13:42:10 +0200
Subject: [PATCH 695/887] update docs

---
 CHANGELOG.md  |  1 +
 README.md     | 14 ++++++++++++--
 docs/usage.md | 22 ++++++++++++++++++----
 3 files changed, 31 insertions(+), 6 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 752d565a2..9c6a723d9 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -46,6 +46,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update STAR-Fusion to 1.15.0 and update nf-core modules [#664](https://github.com/nf-core/rnafusion/pull/664)
 - Update fusionreport 4.0.0 that replaces the score of a fusion with a Fusion Indication Index [#667](https://github.com/nf-core/rnafusion/pull/667)
 - Added a extra trimming step for fusioncatcher in case a different read-length is wished for this tool only [#674](https://github.com/nf-core/rnafusion/pull/674)
+- Added support for references hosted on the nf-core AWS S3 bucket. [#717](https://github.com/nf-core/rnafusion/pull/717)
 
 ### Changed
 
diff --git a/README.md b/README.md
index 0943e2a53..e1dd9c2b5 100644
--- a/README.md
+++ b/README.md
@@ -29,9 +29,19 @@ In rnafusion the full-sized test includes reference building and fusion detectio
 
 ![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)
 
-### Build references
+### References
 
-`--references_only` triggers a workflow to ONLY build references, otherwise the references are build when the analysis is run:
+The references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):
+
+```bash
+aws --no-sign-request s3 sync s3://nf-core-megatests/rnafusion/references/ <path_to_references>
+```
+
+The path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.
+
+:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**
+
+The pipeline is also able to build the references in case files from a specific gencode version are missing. This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.
 
 1. Download gencode fasta and gtf files
 2. Create [STAR](https://github.com/alexdobin/STAR) index
diff --git a/docs/usage.md b/docs/usage.md
index 6a8530c18..aebc5eb26 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -24,10 +24,19 @@ The pipeline is divided into two parts:
 
 The rnafusion pipeline needs references for the fusion detection tools, so downloading these is a **requirement**.
 
-> **IMPORTANT**
->
-> - Note that this step takes about 24 hours to complete on HPC.
-> - Do not provide a samplesheet via the `input` parameter, otherwise the pipeline will run the analysis directly after downloading the references (except if that is what you want).
+The references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):
+
+```bash
+aws --no-sign-request s3 sync s3://nf-core-megatests/rnafusion/references/ <path_to_references>
+```
+
+The path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.
+
+:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**
+
+The fusion report references available on the S3 bucket do not contain information from cosmic due to licensing issues. If you want to use the cosmic database, you will need to build the fusion report references yourself by either deleting the `fusion_report_db` folder in the references directory or by specifying a different location for the fusion report directory with `--fusionreport_ref <PATH/TO/REFERENCES>`. The cosmic username and password should also be given in this case using `--cosmic_username <EMAIL>` and `--cosmic_passwd <PASSWORD>`.
+
+Additionally, the references can be built by the pipeline using the following command:
 
 ```bash
 nextflow run nf-core/rnafusion \
@@ -38,6 +47,11 @@ nextflow run nf-core/rnafusion \
   --outdir <PATH/TO/REFERENCES>
 ```
 
+> **IMPORTANT**
+>
+> - Note that this step takes about 24 hours to complete on HPC.
+> - Do not provide a samplesheet via the `input` parameter, otherwise the pipeline will run the analysis directly after downloading the references (except if that is what you want).
+
 References for each tools can also be downloaded separately with:
 
 ```bash

From e3772614f91c34dd9fde6c9cf1669c78f6d2b15e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 23 Jul 2025 14:09:14 +0200
Subject: [PATCH 696/887] update fusioninspector snap

---
 modules/local/fusioninspector/tests/main.nf.test.snap | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 42ecbe8c4..d73bd00c6 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -398,7 +398,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.tsv:md5,c992c4485f060b1c41be5c29efc4abba"
+                    "test.FusionInspector.fusions.tsv:md5,594d2d3eb7e3a76114f7ca308e0b1e5e"
                 ]
             ],
             [
@@ -416,7 +416,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.abridged.tsv:md5,727aed24358441c63fe08b0e6aaca42b"
+                    "test.FusionInspector.fusions.abridged.tsv:md5,a8bda231a5b90f06a6fc2db8c1cfd762"
                 ]
             ],
             [
@@ -425,7 +425,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "FusionInspector.log:md5,fc708e06807eb0c84a182a11b1c5112c"
+                    "FusionInspector.log:md5,0e4a1e2c37113035d7be5d22044670fc"
                 ]
             ]
         ],
@@ -433,6 +433,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T17:01:54.346734"
+        "timestamp": "2025-07-23T13:52:04.610959"
     }
 }
\ No newline at end of file

From 86fddebb1867ac8f4a52371bb23e66f860d3c3af Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 23 Jul 2025 14:58:19 +0200
Subject: [PATCH 697/887] update snap

---
 modules/local/starfusion/detect/tests/main.nf.test.snap | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/starfusion/detect/tests/main.nf.test.snap b/modules/local/starfusion/detect/tests/main.nf.test.snap
index 55f6c6fee..aec32a9de 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test.snap
+++ b/modules/local/starfusion/detect/tests/main.nf.test.snap
@@ -96,7 +96,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.starfusion.abridged.coding_effect.tsv:md5,760ef75cd5d17fd0adf3733d215fbca6"
+                    "test.starfusion.abridged.coding_effect.tsv:md5,d219ccf8a6178df329fd68cbcf6b804a"
                 ]
             ],
             [
@@ -107,6 +107,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T16:26:23.317406"
+        "timestamp": "2025-07-23T14:48:43.650905"
     }
 }
\ No newline at end of file

From 7a6c37f00f833ae59f564e05318e6d43d9d9fce5 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 24 Jul 2025 09:36:24 +0200
Subject: [PATCH 698/887] fix some wrong includes after merge conflicts

---
 subworkflows/local/build_references/main.nf | 2 --
 1 file changed, 2 deletions(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 1e6878d3a..f62d74fd2 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -5,8 +5,6 @@
 */
 
 include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode_download/main'
-include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/nf-core/fusionreport/download/main'
-include { FUSIONCATCHER_DOWNLOAD }          from '../../../modules/local/fusioncatcher/download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
 include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
 include { GET_RRNA_TRANSCRIPTS }            from '../../../modules/local/get_rrna_transcript/main'

From e704d6545a2a2951f91d5c7d3e7e407c76047025 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 24 Jul 2025 09:50:21 +0200
Subject: [PATCH 699/887] fix config issues

---
 conf/test.config         | 12 +++++++++---
 tests/test_build.nf.test |  2 +-
 2 files changed, 10 insertions(+), 4 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 7e5c591ef..13c8c62e7 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -15,9 +15,15 @@ params {
     config_profile_description = 'Minimal test dataset to check pipeline function'
 
     // Input data
-    input                  = "${projectDir}/tests/csv/fastq.csv"
-    tools                  = "all"
-    no_cosmic              = true
+    input                       = "${projectDir}/tests/csv/fastq.csv"
+    tools                       = "all"
+    no_cosmic                   = true
+    genomes_base                = "${params.outdir}/references"
+    arriba_ref_protein_domains  = null
+    arriba_ref_known_fusions    = null
+    arriba_ref_blacklist        = null
+    arriba_ref_cytobands        = null
+    fusioncatcher_ref           = null
 }
 
 // Limit and standardize resources for github actions and reproducibility
diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index d967dbcd1..6c00607f2 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -29,7 +29,7 @@ nextflow_pipeline {
                 gtf                        = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf'
                 fusion_annot_lib           = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 species                    = 'homo_sapiens'
-                dfam_version               = '3.4'
+                dfam_version               = 3.4
                 outdir                     = "$outputDir"
             }
         }

From 118d1badc7c709c2f02d40c1856c60d28fade429 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Jul 2025 14:28:03 +0200
Subject: [PATCH 700/887] update test to exclude exact match for files with
 dates

---
 docs/output.md                                |   1 +
 .../prepgenomelib/tests/main.nf.test          |  81 +++++++-
 .../prepgenomelib/tests/main.nf.test.snap     | 175 ++++++++----------
 3 files changed, 159 insertions(+), 98 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index e9952b3c2..aec6e6e03 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -280,6 +280,7 @@ The weights for databases are as follows:
 The Fusion Indication Index FII is calculated using two components:
 
 1. Tool Detection (50% of total FII)
+
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
index fd665f6ac..307219a41 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -45,7 +45,86 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
-                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
+                { assert snapshot(
+                    path(process.out.reference[0][1]).resolve("AnnotFilterRule.pm"),
+                    path(process.out.reference[0][1]).resolve("blast_pairs.dat.gz").exists(),
+                    path(process.out.reference[0][1]).resolve("blast_pairs.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("__chkpts/annotfiltrule_cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/blast_pairs.idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/cp_gene_blast_pairs.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/cp_pfam_dat.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/cp_ref_annot_cdna.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/fusion_annot_lib.cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/_fusion_annot_lib.idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/index_pfam_hits.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/index_ref_annot_cdna.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/makeblastdb.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/mm2_genome_idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/mm2.splice_bed.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/_prot_info_db.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.gene_spans.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.mini.sortu.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome_fai.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome.fa.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.cp.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.index.ok"),
+                    path(process.out.reference[0][1]).resolve("__chkpts/validate_ctat_genome_lib.ok"),
+                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.gz"),
+                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("pfam_domains.dbm").exists(),
+                    path(process.out.reference[0][1]).resolve("PFAM.domtblout.dat.gz").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cds").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf"),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.gene_spans").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mini.sortu"),
+                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mm2.splice.bed"),
+                    path(process.out.reference[0][1]).resolve("ref_annot.pep").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_annot.prot_info.dbm").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.fai"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.mm2"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ndb"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nhr"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nin").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.njs").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.not"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nsq"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ntf"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nto"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/build.ok"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrLength.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrNameLength.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrName.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrStart.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonGeTrInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/geneInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Genome"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/genomeParameters.txt").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Log.out").exists(),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
+                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/transcriptInfo.tab"),
+                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
+                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dbm").exists(),
+                    path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
+                    path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz"),
+                    path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz.tbi"),
+                    path(process.out.reference[0][1]).resolve("cancer_splicing_lib/cancer_splicing.idx").exists(),
+                    process.out.version
+                ).match()
+            }
             )
         }
     }
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
index 9aff241a2..9c7581a98 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
@@ -1,108 +1,89 @@
 {
     "test": {
         "content": [
-            {
-                "reference": [
-                    [
-                        {
-                            "id": "minigenome fasta"
-                        },
-                        [
-                            "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-                            "PFAM.domtblout.dat.gz:md5,075c489b35a7fd55b741102769af210f",
-                            [
-                                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                            ],
-                            "blast_pairs.dat.gz:md5,79b51d2021d564aba2bc931d52816114",
-                            "blast_pairs.idx:md5,c2d738b2541366d75d21b3503e5d5121",
-                            [
-                                "cancer_splicing.idx:md5,ca755ab82af1285bfbf945d92a260a6f"
-                            ],
-                            "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                            "fusion_annot_lib.idx:md5,252532308d08ee6c012c69883c343d02",
-                            "pfam_domains.dbm:md5,a5c6c00f2f0759f85bdef39629b3e5d2",
-                            "refGene.bed:md5,2262b9ee22ea50009f4a8c51c6b2fac7",
-                            "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
-                            "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
-                            "ref_annot.cdna.fa:md5,b087fb1b388d75a88887bb98d8c5d339",
-                            "ref_annot.cdna.fa.idx:md5,d6c43237e5c522278f944edc79b5c567",
-                            "ref_annot.cds:md5,3686879a92b12a36ee89fcb4dfc9d9f8",
-                            "ref_annot.cdsplus.fa:md5,72561309cba7f598c641c19df84e9c0e",
-                            "ref_annot.cdsplus.fa.idx:md5,5751b6d5373f24fd98a2ce86618e3b7c",
-                            "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                            "ref_annot.gtf.gene_spans:md5,96105e687b1c346c773abc5915d817b2",
-                            "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
-                            "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                            "ref_annot.pep:md5,28d1d869dcd616da362ead8ae39df21d",
-                            "ref_annot.prot_info.dbm:md5,18abe5ec2717d4c214d7546b998dfe44",
-                            "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
-                            "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
-                            "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
-                            "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
-                            "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                            "ref_genome.fa.nin:md5,d4d9344b71466f067e9a3d8043feb891",
-                            "ref_genome.fa.njs:md5,2b049589cf2e3ab830883dda45ffdf3c",
-                            "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
-                            "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
-                            "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
-                            "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
-                            [
-                                "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                                "Log.out:md5,a352597c0bed7b2ae1018532a5893203",
-                                "SA:md5,7dd9083264be9c6a2194d990bc10d237",
-                                "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
-                                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
-                                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
-                                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
-                                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
-                                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
-                                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
-                                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                                "genomeParameters.txt:md5,09b8b0bb918b2c628030d9fb1aa7aea6",
-                                "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
-                                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
-                                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
-                                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2"
-                            ],
-                            "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                            "trans.blast.align_coords.align_coords.dbm:md5,776d70b98b4be1290e3e187b5fef3a80",
-                            "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
-                        ]
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,8175cd19f5d5b8b2214cd948120d7524"
-                ]
-            }
+            "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
+            true,
+            true,
+            "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+            true,
+            true,
+            true,
+            true,
+            true,
+            true,
+            true,
+            true,
+            "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+            true,
+            "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+            "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+            true,
+            true,
+            "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+            "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+            "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+            "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+            "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+            true,
+            true,
+            "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+            "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+            "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+            "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+            true,
+            "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+            "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+            "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+            "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+            "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+            "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+            "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+            "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+            true,
+            true,
+            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+            "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+            "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+            "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+            "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+            true,
+            "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
+            "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
+            "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
+            true,
+            null
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-23T08:54:58.892241"
+        "timestamp": "2025-07-24T14:26:34.908635"
     },
     "test - stub": {
         "content": [

From 426bb5142678dfb54e3b4955e463f4e6c329e1d9 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Jul 2025 15:51:36 +0200
Subject: [PATCH 701/887] update input to STARFUSION BUILD

---
 .../prepgenomelib/tests/main.nf.test             | 16 ++++++++++++----
 modules/local/fusioninspector/tests/main.nf.test | 11 +++++++++--
 2 files changed, 21 insertions(+), 6 deletions(-)

diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
index 3701f9409..1c6c2754a 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -18,10 +18,18 @@ nextflow_process {
                     file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
                 ]
 
-                input [2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
-                input [3] = "homo_sapiens"
-                input [4] = "3.8"
-                input [5] = "37.4"                """
+                input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input[3] = "homo_sapiens"
+                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[5] = [
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                ]
+                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                """
             }
         }
     }
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 8868b847b..d5d8f260c 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -26,8 +26,15 @@ nextflow_process {
 
                     input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
                     input[3] = "homo_sapiens"
-                    input [4] = "3.8"
-                    input [5] = "37.4"
+                    input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                    input[5] = [
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                    ]
+                    input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                     """
                 }
             }

From 1a6c91e681dd69ad6dd0c7ea0483bc517332d7f6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 24 Jul 2025 15:58:45 +0200
Subject: [PATCH 702/887] update prettier

---
 docs/output.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index aec6e6e03..e9952b3c2 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -280,7 +280,6 @@ The weights for databases are as follows:
 The Fusion Indication Index FII is calculated using two components:
 
 1. Tool Detection (50% of total FII)
-
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 

From 4cc9c03d2fa64311ba1528f779a5dcc5c885ec0e Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Fri, 25 Jul 2025 13:01:49 +0200
Subject: [PATCH 703/887] Update README.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index e1dd9c2b5..c4ebb9c73 100644
--- a/README.md
+++ b/README.md
@@ -34,7 +34,7 @@ In rnafusion the full-sized test includes reference building and fusion detectio
 The references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):
 
 ```bash
-aws --no-sign-request s3 sync s3://nf-core-megatests/rnafusion/references/ <path_to_references>
+aws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>
 ```
 
 The path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.

From 1041607c51c557c9eaaff7075b2e05bafd499c08 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 25 Jul 2025 13:03:09 +0200
Subject: [PATCH 704/887] fix another docs typo

---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index aebc5eb26..6be6aa0c3 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -27,7 +27,7 @@ The rnafusion pipeline needs references for the fusion detection tools, so downl
 The references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):
 
 ```bash
-aws --no-sign-request s3 sync s3://nf-core-megatests/rnafusion/references/ <path_to_references>
+aws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>
 ```
 
 The path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.

From 7fe1607034df8ee25705c824eb52be588cfefd17 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Jul 2025 15:25:35 +0200
Subject: [PATCH 705/887] update ctat test

---
 docs/output.md                                |   1 +
 .../prepgenomelib/tests/main.nf.test          | 176 +++++++++---------
 .../prepgenomelib/tests/main.nf.test.snap     | 103 +++++-----
 3 files changed, 138 insertions(+), 142 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index e9952b3c2..aec6e6e03 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -280,6 +280,7 @@ The weights for databases are as follows:
 The Fusion Indication Index FII is calculated using two components:
 
 1. Tool Detection (50% of total FII)
+
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
index 1c6c2754a..5486cf39e 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -10,15 +10,15 @@ nextflow_process {
             process {
                 """
                 input[0] = [
-                    [ id:'minigenome fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                    [ id:'minigenome_fasta' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
                 ]
                 input[1] = [
-                    [ id:'minigenome gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                    [ id:'minigenome_gtf' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
                 ]
 
-                input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                 input[3] = "homo_sapiens"
                 input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
                 input[5] = [
@@ -37,9 +37,6 @@ nextflow_process {
     test("test") {
 
         when {
-            params {
-                outdir = "$outputDir"
-            }
             process {
                 """
                 input[0] = STARFUSION_BUILD.out.reference
@@ -51,92 +48,89 @@ nextflow_process {
         }
 
         then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(
-                    path(process.out.reference[0][1]).resolve("AnnotFilterRule.pm"),
-                    path(process.out.reference[0][1]).resolve("blast_pairs.dat.gz").exists(),
-                    path(process.out.reference[0][1]).resolve("blast_pairs.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("__chkpts/annotfiltrule_cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/blast_pairs.idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/cp_gene_blast_pairs.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/cp_pfam_dat.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/cp_ref_annot_cdna.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/fusion_annot_lib.cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/_fusion_annot_lib.idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/index_pfam_hits.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/index_ref_annot_cdna.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/makeblastdb.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/mm2_genome_idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/mm2.splice_bed.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/_prot_info_db.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.gene_spans.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.mini.sortu.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_annot.gtf.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome_fai.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/ref_genome.fa.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.cp.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/trans.blast.dat.index.ok"),
-                    path(process.out.reference[0][1]).resolve("__chkpts/validate_ctat_genome_lib.ok"),
-                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.gz"),
-                    path(process.out.reference[0][1]).resolve("fusion_annot_lib.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("pfam_domains.dbm").exists(),
-                    path(process.out.reference[0][1]).resolve("PFAM.domtblout.dat.gz").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdna.fa.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cds").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.cdsplus.fa.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf"),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.gene_spans").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mini.sortu"),
-                    path(process.out.reference[0][1]).resolve("ref_annot.gtf.mm2.splice.bed"),
-                    path(process.out.reference[0][1]).resolve("ref_annot.pep").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_annot.prot_info.dbm").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.fai"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.mm2"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ndb"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nhr"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nin").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.njs").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.not"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nsq"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.ntf"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.nto"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/build.ok"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrLength.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrNameLength.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrName.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrStart.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonGeTrInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/geneInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Genome"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/genomeParameters.txt").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Log.out").exists(),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
-                    path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/transcriptInfo.tab"),
-                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
-                    path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dbm").exists(),
-                    path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
-                    path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz"),
-                    path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz.tbi"),
-                    path(process.out.reference[0][1]).resolve("cancer_splicing_lib/cancer_splicing.idx").exists(),
-                    process.out.version
+            def outputDir = file(process.out.reference[0][1])
+            assert snapshot(
+                path(process.out.reference[0][1]).resolve("AnnotFilterRule.pm"),
+                file("$outputDir/blast_pairs.dat.gz").name,
+                file("$outputDir/blast_pairs.idx").name,
+                file("$outputDir/__chkpts/annotfiltrule_cp.ok").name,
+                file("$outputDir/__chkpts/blast_pairs.idx.ok").name,
+                file("$outputDir/__chkpts/cp_gene_blast_pairs.ok").name,
+                file("$outputDir/__chkpts/cp_pfam_dat.ok").name,
+                file("$outputDir/__chkpts/cp_ref_annot_cdna.ok").name,
+                file("$outputDir/__chkpts/fusion_annot_lib.cp.ok").name,
+                file("$outputDir/__chkpts/_fusion_annot_lib.idx.ok").name,
+                file("$outputDir/__chkpts/index_pfam_hits.ok").name,
+                file("$outputDir/__chkpts/index_ref_annot_cdna.ok").name,
+                file("$outputDir/__chkpts/makeblastdb.ok").name,
+                file("$outputDir/__chkpts/mm2_genome_idx.ok").name,
+                file("$outputDir/__chkpts/mm2.splice_bed.ok").name,
+                file("$outputDir/__chkpts/_prot_info_db.ok").name,
+                file("$outputDir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok").name,
+                file("$outputDir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok").name,
+                file("$outputDir/__chkpts/ref_annot.gtf.gene_spans.ok").name,
+                file("$outputDir/__chkpts/ref_annot.gtf.mini.sortu.ok").name,
+                file("$outputDir/__chkpts/ref_annot.gtf.ok").name,
+                file("$outputDir/__chkpts/ref_genome_fai.ok").name,
+                file("$outputDir/__chkpts/ref_genome.fa.ok").name,
+                file("$outputDir/__chkpts/trans.blast.dat.cp.ok").name,
+                file("$outputDir/__chkpts/trans.blast.dat.index.ok").name,
+                file("$outputDir/__chkpts/validate_ctat_genome_lib.ok").name,
+                path(process.out.reference[0][1]).resolve("fusion_annot_lib.gz"),
+                file("$outputDir/fusion_annot_lib.idx").name,
+                file("$outputDir/pfam_domains.dbm").name,
+                file("$outputDir/PFAM.domtblout.dat.gz").name,
+                file("$outputDir/ref_annot.cdna.fa").name,
+                file("$outputDir/ref_annot.cdna.fa.idx").name,
+                file("$outputDir/ref_annot.cds").name,
+                file("$outputDir/ref_annot.cdsplus.fa").name,
+                file("$outputDir/ref_annot.cdsplus.fa.idx").name,
+                path(process.out.reference[0][1]).resolve("ref_annot.gtf"),
+                file("$outputDir/ref_annot.gtf.gene_spans").name,
+                path(process.out.reference[0][1]).resolve("ref_annot.gtf.mini.sortu"),
+                path(process.out.reference[0][1]).resolve("ref_annot.gtf.mm2.splice.bed"),
+                file("$outputDir/ref_annot.pep").name,
+                file("$outputDir/ref_annot.prot_info.dbm").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.fai"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.mm2"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.ndb"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.nhr"),
+                file("$outputDir/ref_genome.fa.nin").name,
+                file("$outputDir/ref_genome.fa.njs").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.not"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.nsq"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.ntf"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.nto"),
+                file("$outputDir/ref_genome.fa.star.idx").name,
+                file("$outputDir/ref_genome.fa.star.idx/build.ok").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrLength.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrNameLength.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrName.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrStart.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonGeTrInfo.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonInfo.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/geneInfo.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Genome"),
+                file("$outputDir/ref_genome.fa.star.idx/genomeParameters.txt").name,
+                file("$outputDir/ref_genome.fa.star.idx/Log.out").name,
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
+                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/transcriptInfo.tab"),
+                path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
+                file("$outputDir/trans.blast.align_coords.align_coords.dbm").name,
+                path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
+                path(process.out.reference[0][1]).resolve("refGene.bed"),
+                path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz"),
+                path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz.tbi"),
+                path(process.out.reference[0][1]).resolve("cancer_splicing_lib/cancer_splicing.idx").exists(),
+                process.out.version
                 ).match()
-            }
-            )
         }
     }
-
     test("test - stub") {
 
         options "-stub"
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
index 9c7581a98..4480ad1f7 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
@@ -2,59 +2,59 @@
     "test": {
         "content": [
             "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-            true,
-            true,
-            "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-            "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "blast_pairs.dat.gz",
+            "blast_pairs.idx",
+            "annotfiltrule_cp.ok",
+            "blast_pairs.idx.ok",
+            "cp_gene_blast_pairs.ok",
+            "cp_pfam_dat.ok",
+            "cp_ref_annot_cdna.ok",
+            "fusion_annot_lib.cp.ok",
+            "_fusion_annot_lib.idx.ok",
+            "index_pfam_hits.ok",
+            "index_ref_annot_cdna.ok",
+            "makeblastdb.ok",
+            "mm2_genome_idx.ok",
+            "mm2.splice_bed.ok",
+            "_prot_info_db.ok",
+            "ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+            "ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+            "ref_annot.gtf.gene_spans.ok",
+            "ref_annot.gtf.mini.sortu.ok",
+            "ref_annot.gtf.ok",
+            "ref_genome_fai.ok",
+            "ref_genome.fa.ok",
+            "trans.blast.dat.cp.ok",
+            "trans.blast.dat.index.ok",
+            "validate_ctat_genome_lib.ok",
             "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-            true,
-            true,
-            true,
-            true,
-            true,
-            true,
-            true,
-            true,
+            "fusion_annot_lib.idx",
+            "pfam_domains.dbm",
+            "PFAM.domtblout.dat.gz",
+            "ref_annot.cdna.fa",
+            "ref_annot.cdna.fa.idx",
+            "ref_annot.cds",
+            "ref_annot.cdsplus.fa",
+            "ref_annot.cdsplus.fa.idx",
             "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-            true,
+            "ref_annot.gtf.gene_spans",
             "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
             "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-            true,
-            true,
+            "ref_annot.pep",
+            "ref_annot.prot_info.dbm",
             "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
             "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
             "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
             "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
             "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-            true,
-            true,
+            "ref_genome.fa.nin",
+            "ref_genome.fa.njs",
             "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
             "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
             "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
             "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
-            true,
-            "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+            "ref_genome.fa.star.idx",
+            "build.ok",
             "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
             "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
             "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
@@ -63,8 +63,8 @@
             "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
             "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
             "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-            true,
-            true,
+            "genomeParameters.txt",
+            "Log.out",
             "SA:md5,7dd9083264be9c6a2194d990bc10d237",
             "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
             "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
@@ -72,8 +72,9 @@
             "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
             "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
             "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-            true,
+            "trans.blast.align_coords.align_coords.dbm",
             "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
+            "refGene.bed:md5,ae9d27b8f4efb1e57dccddf97be0f329",
             "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
             "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
             true,
@@ -83,7 +84,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-24T14:26:34.908635"
+        "timestamp": "2025-07-25T15:06:29.346013"
     },
     "test - stub": {
         "content": [
@@ -91,11 +92,11 @@
                 "reference": [
                     [
                         {
-                            "id": "minigenome fasta"
+                            "id": "minigenome_fasta"
                         },
                         [
                             "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "PFAM.domtblout.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             [
                                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -121,12 +122,12 @@
                                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
                             ],
-                            "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "blast_pairs.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                             [
                                 "cancer_splicing.idx:md5,d41d8cd98f00b204e9800998ecf8427e"
                             ],
-                            "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                            "fusion_annot_lib.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
                             "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -175,7 +176,7 @@
                             ],
                             "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
                             "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
+                            "trans.blast.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
                         ]
                     ]
                 ],
@@ -186,8 +187,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "24.10.5"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-03-19T09:17:05.045966772"
+        "timestamp": "2025-07-25T15:18:45.370275"
     }
 }
\ No newline at end of file

From 29185bd7513790ac77ecef9df8881b4db79586a8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 25 Jul 2025 15:46:47 +0200
Subject: [PATCH 706/887] prettier

---
 docs/output.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index aec6e6e03..e9952b3c2 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -280,7 +280,6 @@ The weights for databases are as follows:
 The Fusion Indication Index FII is calculated using two components:
 
 1. Tool Detection (50% of total FII)
-
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 

From 9d3fb7ecfc620a5bd8c22f8dcd495a1618526a1a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Sat, 26 Jul 2025 20:32:24 +0200
Subject: [PATCH 707/887] update ctat test

---
 modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test   | 2 +-
 .../local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap  | 4 ++--
 modules/local/fusioninspector/tests/main.nf.test              | 2 +-
 3 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
index 5486cf39e..1923fdd1a 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -123,7 +123,7 @@ nextflow_process {
                 path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
                 file("$outputDir/trans.blast.align_coords.align_coords.dbm").name,
                 path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
-                path(process.out.reference[0][1]).resolve("refGene.bed"),
+                path(process.out.reference[0][1]).resolve("refGene.bed").exists(),
                 path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz"),
                 path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz.tbi"),
                 path(process.out.reference[0][1]).resolve("cancer_splicing_lib/cancer_splicing.idx").exists(),
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
index 4480ad1f7..80739989c 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
@@ -74,7 +74,7 @@
             "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
             "trans.blast.align_coords.align_coords.dbm",
             "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
-            "refGene.bed:md5,ae9d27b8f4efb1e57dccddf97be0f329",
+            true,
             "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
             "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
             true,
@@ -84,7 +84,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-25T15:06:29.346013"
+        "timestamp": "2025-07-26T20:17:06.519409"
     },
     "test - stub": {
         "content": [
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index d5d8f260c..611a9dea0 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -12,7 +12,7 @@ nextflow_process {
 
         setup {
             run("STARFUSION_BUILD") {
-                script "../../../../nf-core/starfusion/build/main.nf"
+                script "../../../nf-core/starfusion/build/main.nf"
                 process {
                     """
                     input[0] = [

From de664dc3f917a2c6b49121aa02eda42df3527c0f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Sat, 26 Jul 2025 21:33:19 +0200
Subject: [PATCH 708/887] update starfusion detect test

---
 modules/local/starfusion/detect/tests/main.nf.test   |  2 +-
 .../local/starfusion/detect/tests/main.nf.test.snap  | 12 ++----------
 2 files changed, 3 insertions(+), 11 deletions(-)

diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
index c8bf4e119..599d771f3 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test
+++ b/modules/local/starfusion/detect/tests/main.nf.test
@@ -57,7 +57,7 @@ nextflow_process {
             assert snapshot(
                 process.out.fusions,
                 process.out.abridged,
-                process.out.coding_effect,
+                path(process.out.coding_effect[0][1]).resolve("*.coding_effect.tsv").exists(),
                 process.out.versions
             ).match()
         }
diff --git a/modules/local/starfusion/detect/tests/main.nf.test.snap b/modules/local/starfusion/detect/tests/main.nf.test.snap
index aec32a9de..3fe34cbc2 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test.snap
+++ b/modules/local/starfusion/detect/tests/main.nf.test.snap
@@ -90,15 +90,7 @@
                     "test.starfusion.abridged.tsv:md5,d6d20fdd4b5cba21b9c0ebf8e0ea19ff"
                 ]
             ],
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.starfusion.abridged.coding_effect.tsv:md5,d219ccf8a6178df329fd68cbcf6b804a"
-                ]
-            ],
+            false,
             [
                 "versions.yml:md5,1597cbbd06bf7278faa9c6282665cbac"
             ]
@@ -107,6 +99,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-23T14:48:43.650905"
+        "timestamp": "2025-07-26T21:15:38.318797"
     }
 }
\ No newline at end of file

From 7efcefe4c62e6c2c722e531ecd3c0a29c40d0a0a Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Jul 2025 10:44:58 +0200
Subject: [PATCH 709/887] update fusioninspector

---
 modules/local/fusioninspector/tests/main.nf.test      | 10 +++++-----
 modules/local/fusioninspector/tests/main.nf.test.snap |  8 ++++----
 2 files changed, 9 insertions(+), 9 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 611a9dea0..8cbe94f4e 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -16,15 +16,15 @@ nextflow_process {
                 process {
                     """
                     input[0] = [
-                        [ id:'minigenome fasta' ],
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                        [ id:'minigenome_fasta' ],
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
                     ]
                     input[1] = [
-                        [ id:'minigenome gtf' ],
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                        [ id:'minigenome_gtf' ],
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
                     ]
 
-                    input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                    input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                     input[3] = "homo_sapiens"
                     input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
                     input[5] = [
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index d73bd00c6..5083f66a7 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -398,7 +398,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.tsv:md5,594d2d3eb7e3a76114f7ca308e0b1e5e"
+                    "test.FusionInspector.fusions.tsv:md5,25af7c8a33eae6d6af4f6bfe4abb9c4c"
                 ]
             ],
             [
@@ -416,7 +416,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.abridged.tsv:md5,a8bda231a5b90f06a6fc2db8c1cfd762"
+                    "test.FusionInspector.fusions.abridged.tsv:md5,a985145b60f2493c91598cf0eb739900"
                 ]
             ],
             [
@@ -425,7 +425,7 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "FusionInspector.log:md5,0e4a1e2c37113035d7be5d22044670fc"
+                    "FusionInspector.log:md5,924b6ba4831c4fd9611b8026ec7905fa"
                 ]
             ]
         ],
@@ -433,6 +433,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-23T13:52:04.610959"
+        "timestamp": "2025-07-28T10:19:42.587045"
     }
 }
\ No newline at end of file

From 219647884cbe21c089bca6cd73a4af84c690bc61 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Jul 2025 11:38:32 +0200
Subject: [PATCH 710/887] change logic

---
 subworkflows/local/fusioninspector_workflow.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index 873732146..2dd594b42 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -47,8 +47,8 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
         if (
             !skip_vcf &&
-            tsv_nonempty &&
-            gtf_nonempty
+            FUSIONINSPECTOR.out.tsv[0][1].file.exists() && FUSIONINSPECTOR.out.tsv[0][1].file.size() > 0 &&
+            FUSIONINSPECTOR.out.out_gtf[0][1].file.exists() && FUSIONINSPECTOR.out.out_gtf[0][1].file.size() > 0
         ) {
             AGAT_CONVERTSPGFF2TSV(FUSIONINSPECTOR.out.out_gtf)
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)

From 3613777e731ceb7d8ecd8685f6b8c9d86f85160d Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Jul 2025 12:55:34 +0200
Subject: [PATCH 711/887] update fusioninspector test

---
 .../local/fusioninspector/tests/main.nf.test  |  5 ++--
 .../fusioninspector/tests/main.nf.test.snap   | 25 ++++---------------
 2 files changed, 8 insertions(+), 22 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 8cbe94f4e..8f98631b5 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -62,10 +62,11 @@ nextflow_process {
             assertAll(
                 { assert process.success },
                 { assert snapshot(
-                    process.out.tsv,
+                    path(process.out.tsv[0][1]).exists(),
+                    path(process.out.abridged_tsv[0][1]).exists(),
                     process.out.out_gtf,
                     process.out.abridged_tsv,
-                    process.out.log,
+                    path(process.out.abridged_tsv[0][1]).exists(),
                 ).match() }
             )
         }
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 5083f66a7..cde32193c 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -392,15 +392,8 @@
     },
     "FUSIONINSPECTOR": {
         "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.FusionInspector.fusions.tsv:md5,25af7c8a33eae6d6af4f6bfe4abb9c4c"
-                ]
-            ],
+            true,
+            true,
             [
                 [
                     {
@@ -416,23 +409,15 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.FusionInspector.fusions.abridged.tsv:md5,a985145b60f2493c91598cf0eb739900"
+                    "test.FusionInspector.fusions.abridged.tsv:md5,2097101e06c55ed70e73429a81330682"
                 ]
             ],
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "FusionInspector.log:md5,924b6ba4831c4fd9611b8026ec7905fa"
-                ]
-            ]
+            true
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-28T10:19:42.587045"
+        "timestamp": "2025-07-28T12:29:41.961289"
     }
 }
\ No newline at end of file

From a3843eeeebc60d050f20dbbf246610003382f5e6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Jul 2025 13:06:25 +0200
Subject: [PATCH 712/887] udpate logic

---
 subworkflows/local/fusioninspector_workflow.nf | 12 +++++++-----
 1 file changed, 7 insertions(+), 5 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index 2dd594b42..1395a56d0 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -43,22 +43,24 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
 
         def tsv_nonempty = FUSIONINSPECTOR.out.tsv.filter { meta, file -> file.exists() && file.size() > 0 }
+        def tsv_abridged_nonempty = FUSIONINSPECTOR.out.abridged_tsv.filter { meta, file -> file.exists() && file.size() > 0 }
+
         def gtf_nonempty = FUSIONINSPECTOR.out.out_gtf.filter { meta, file -> file.exists() && file.size() > 0 }
 
         if (
             !skip_vcf &&
-            FUSIONINSPECTOR.out.tsv[0][1].file.exists() && FUSIONINSPECTOR.out.tsv[0][1].file.size() > 0 &&
-            FUSIONINSPECTOR.out.out_gtf[0][1].file.exists() && FUSIONINSPECTOR.out.out_gtf[0][1].file.size() > 0
+            tsv_nonempty &&
+            gtf_nonempty
         ) {
-            AGAT_CONVERTSPGFF2TSV(FUSIONINSPECTOR.out.out_gtf)
+            AGAT_CONVERTSPGFF2TSV(gtf_nonempty)
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
-            fusion_data = FUSIONINSPECTOR.out.abridged_tsv.join(AGAT_CONVERTSPGFF2TSV.out.tsv).join(fusionreport_out).join(fusionreport_csv)
+            fusion_data = tsv_abridged_nonempty.join(AGAT_CONVERTSPGFF2TSV.out.tsv).join(fusionreport_out).join(fusionreport_csv)
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)
 
-            ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(FUSIONINSPECTOR.out.tsv)
+            ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(tsv_nonempty)
             ARRIBA_VISUALISATION(
                 ch_bam_sorted_indexed_fusions,
                 ch_gtf,

From 22036441909f1685e41d52ed0d027010798644bf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Jul 2025 15:00:12 +0200
Subject: [PATCH 713/887] update fusioncatcher test

---
 docs/output.md                                        |  1 -
 modules/local/fusioninspector/tests/main.nf.test      |  3 +--
 modules/local/fusioninspector/tests/main.nf.test.snap | 11 +----------
 3 files changed, 2 insertions(+), 13 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index e9952b3c2..24c6a2d8d 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -395,7 +395,6 @@ The STAR index is generated with `--sjdbOverhang ${params.read_length - 1}`, `pa
 - `starfusion`
   - `<sample>.starfusion.fusion_predictions.tsv` - contains the identified fusions
   - `<sample>.starfusion.abridged.tsv` - contains the identified fusions abridged
-  - `starfusion.abridged.coding_effect.tsv`
 
 </details>
 
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index 8f98631b5..e1a42c1b1 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -65,8 +65,7 @@ nextflow_process {
                     path(process.out.tsv[0][1]).exists(),
                     path(process.out.abridged_tsv[0][1]).exists(),
                     process.out.out_gtf,
-                    process.out.abridged_tsv,
-                    path(process.out.abridged_tsv[0][1]).exists(),
+                    path(process.out.log[0][1]).exists(),
                 ).match() }
             )
         }
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index cde32193c..79e97ea6e 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -403,21 +403,12 @@
                     "test.gtf:md5,d523061bd8b443a014d4cca9406ec772"
                 ]
             ],
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.FusionInspector.fusions.abridged.tsv:md5,2097101e06c55ed70e73429a81330682"
-                ]
-            ],
             true
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-28T12:29:41.961289"
+        "timestamp": "2025-07-28T14:58:15.527794"
     }
 }
\ No newline at end of file

From dd3171a2573f64c9957ddec30a1889ed58422c15 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 28 Jul 2025 15:09:39 +0200
Subject: [PATCH 714/887] recreate transcript version from Transcript id

---
 bin/vcf_collect.py | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py
index 1decbe907..bd9c03351 100755
--- a/bin/vcf_collect.py
+++ b/bin/vcf_collect.py
@@ -89,6 +89,8 @@ def vcf_collect(
         | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0))
     ]
 
+    all_df["transcript_version"] = all_df["Transcript_id"].astype(str).str.split(".").str[-1]
+
     all_df.replace("", np.nan, inplace=True)
     all_df = all_df.drop_duplicates()
 

From f805cb8e2c9f40179c265ebf02207ea20112e1f1 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 29 Jul 2025 12:56:22 +0200
Subject: [PATCH 715/887] fix linting

---
 ro-crate-metadata.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index b865b7385..50b780538 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### Build references\n\n`--references_only` triggers a workflow to ONLY build references, otherwise the references are build when the analysis is run:\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing. This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"

From c8468af7dcb31ce819969bcc7cba02ae7f0a1083 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 29 Jul 2025 14:31:07 +0200
Subject: [PATCH 716/887] update vcf

---
 bin/vcf_collect.py | 29 +++++++++++++++--------------
 1 file changed, 15 insertions(+), 14 deletions(-)

diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py
index bd9c03351..bb5401c8c 100755
--- a/bin/vcf_collect.py
+++ b/bin/vcf_collect.py
@@ -89,23 +89,22 @@ def vcf_collect(
         | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0))
     ]
 
-    all_df["transcript_version"] = all_df["Transcript_id"].astype(str).str.split(".").str[-1]
+    all_df["Left_transcript_version"] = all_df["CDS_LEFT_ID"].astype(str).str.split(".").str[-1]
 
     all_df.replace("", np.nan, inplace=True)
     all_df = all_df.drop_duplicates()
 
-    all_df[["exon_number", "transcript_version"]] = all_df[
-        ["exon_number", "transcript_version"]
+    all_df[["exon_number", "Left_transcript_version"]] = all_df[
+        ["exon_number", "Left_transcript_version"]
     ].replace(0, np.nan)
     # Fill non-empty values within each group for 'exon_number' and 'transcript_version'
     all_df["exon_number"] = all_df.groupby("PosA")["exon_number"].transform(
         lambda x: x.fillna(method="ffill").fillna(method="bfill")
     )
-    all_df["transcript_version"] = all_df.groupby("PosA")[
-        "transcript_version"
+    all_df["Left_transcript_version"] = all_df.groupby("PosA")[
+        "Left_transcript_version"
     ].transform(lambda x: x.fillna(method="ffill").fillna(method="bfill"))
 
-    all_df = all_df.rename(columns={"transcript_version": "Left_transcript_version"})
     all_df = all_df.rename(columns={"exon_number": "Left_exon_number"})
     all_df = all_df[
         [
@@ -156,18 +155,20 @@ def vcf_collect(
     all_df[["PosA", "PosB"]] = all_df[["PosA", "PosB"]].replace(0, np.nan)
     all_df = all_df.replace("", np.nan)
 
-    all_df[["exon_number", "transcript_version"]] = all_df[
-        ["exon_number", "transcript_version"]
+    all_df["Right_transcript_version"] = all_df["CDS_RIGHT_ID"].astype(str).str.split(".").str[-1]
+
+
+    all_df[["exon_number", "Right_transcript_version"]] = all_df[
+        ["exon_number", "Right_transcript_version"]
     ].replace(0, np.nan)
     # Fill non-empty values within each group for 'exon_number' and 'transcript_version'
     all_df["exon_number"] = all_df.groupby("PosB")["exon_number"].transform(
         lambda x: x.fillna(method="ffill").fillna(method="bfill")
     )
-    all_df["transcript_version"] = all_df.groupby("PosB")[
-        "transcript_version"
+    all_df["Right_transcript_version"] = all_df.groupby("PosB")[
+        "Right_transcript_version"
     ].transform(lambda x: x.fillna(method="ffill").fillna(method="bfill"))
 
-    all_df = all_df.rename(columns={"transcript_version": "Right_transcript_version"})
     all_df = all_df.rename(columns={"exon_number": "Right_exon_number"})
 
     all_df = all_df[
@@ -495,10 +496,10 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame:
     df["Left_exon_number"] = df["Left_exon_number"].fillna(0).astype(int).astype(str)
     df["Right_exon_number"] = df["Right_exon_number"].fillna(0).astype(int).astype(str)
     df["Left_transcript_version"] = (
-        df["Left_transcript_version"].fillna(0).astype(int).astype(str)
+        pd.to_numeric(df["Left_transcript_version"], errors="coerce").fillna(0).astype(int).astype(str)
     )
     df["Right_transcript_version"] = (
-        df["Right_transcript_version"].fillna(0).astype(int).astype(str)
+        pd.to_numeric(df["Right_transcript_version"], errors="coerce").fillna(0).astype(int).astype(str)
     )
     df["PosA"] = df["PosA"].fillna(0).astype(int).astype(str)
     df["PosB"] = df["PosB"].fillna(0).astype(int).astype(str)
@@ -581,7 +582,7 @@ def build_gtf_dataframe(file: str) -> pd.DataFrame:
         "orig_coord_info"
     ].str.split(",", expand=True)
     return df[
-        ["Transcript_id", "transcript_version", "exon_number", "orig_start", "orig_end"]
+        ["Transcript_id", "exon_number", "orig_start", "orig_end"]
     ]
 
 

From 95d81c1254e22dd670054fe91915443b54121259 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 29 Jul 2025 14:35:16 +0200
Subject: [PATCH 717/887] update vcf

---
 CHANGELOG.md                      | 1 +
 modules/local/vcf_collect/main.nf | 2 ++
 2 files changed, 3 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index cbd690d4e..09d92ecaa 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -108,6 +108,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix fusioncatcher download link [#693](https://github.com/nf-core/rnafusion/pull/693)
 - Fix fusionreport singularity container [#713](https://github.com/nf-core/rnafusion/pull/713)
 - Fix CTAT-SPLICING output when no cancer introns were found [#722](https://github.com/nf-core/rnafusion/pull/722)
+- Update VCF_COLLECT script to adapt to transcript_version not being an entry in fusioninspector gtf anymore ()
 
 ### Removed
 
diff --git a/modules/local/vcf_collect/main.nf b/modules/local/vcf_collect/main.nf
index 3f218b66b..2968c67b3 100644
--- a/modules/local/vcf_collect/main.nf
+++ b/modules/local/vcf_collect/main.nf
@@ -28,6 +28,7 @@ process VCF_COLLECT {
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         python: \$(python --version | sed 's/Python //g')
+        vcf_collect: 0.1
         HGNC DB retrieval: \$(cat $hgnc_date)
     END_VERSIONS
     """
@@ -40,6 +41,7 @@ process VCF_COLLECT {
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         python: \$(python --version | sed 's/Python //g')
+        vcf_collect: 0.1
     END_VERSIONS
     """
 }

From 7341a690080c1b86c0fed5d702abb52abde504d3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 29 Jul 2025 14:37:49 +0200
Subject: [PATCH 718/887] update changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 09d92ecaa..906d8384b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -108,7 +108,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix fusioncatcher download link [#693](https://github.com/nf-core/rnafusion/pull/693)
 - Fix fusionreport singularity container [#713](https://github.com/nf-core/rnafusion/pull/713)
 - Fix CTAT-SPLICING output when no cancer introns were found [#722](https://github.com/nf-core/rnafusion/pull/722)
-- Update VCF_COLLECT script to adapt to transcript_version not being an entry in fusioninspector gtf anymore ()
+- Update VCF_COLLECT script to adapt to transcript_version not being an entry in fusioninspector gtf anymore [#726](https://github.com/nf-core/rnafusion/pull/726)
 
 ### Removed
 

From 5833f625c398fdc78c0214ab66f4863f0463d392 Mon Sep 17 00:00:00 2001
From: nvnieuwk <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 29 Jul 2025 13:05:17 +0000
Subject: [PATCH 719/887] update build tests

---
 conf/modules.config           |   1 +
 tests/test_build.nf.test.snap | 190 +++++++++++++++++-----------------
 2 files changed, 95 insertions(+), 96 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 7229af944..e15c3914d 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -300,6 +300,7 @@ process {
         cpus   = { 24    * task.attempt }
         memory = { 150.GB * task.attempt }
         time   = { 2.d  * task.attempt }
+        ext.prefix = "ctat"
         publishDir = [
             path: { "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/starfusion" },
             mode: params.publish_dir_mode,
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 9583f9bf3..4c35f5e92 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -59,7 +59,6 @@
                 "references/GRCh38",
                 "references/GRCh38/fusion_report_db",
                 "references/GRCh38/fusion_report_db/DB-timestamp.txt",
-                "references/GRCh38/fusion_report_db/cosmic.db",
                 "references/GRCh38/fusion_report_db/fusion_report.log",
                 "references/GRCh38/fusion_report_db/fusiongdb2.db",
                 "references/GRCh38/fusion_report_db/mitelman.db",
@@ -191,48 +190,47 @@
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cosmic.db:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "DB-timestamp.txt:md5,47763915c2614cf9190457bf6916a677",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusiongdb2.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "mitelman.db:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.gtf.fasta:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.gtf.interval_list:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.gtf.refflat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "complete_ref_lens.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ctable.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ctg_offsets.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "duplicate_clusters.tsv:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "info.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "mphf.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "pos.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "pre_indexing.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "rank.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refAccumLengths.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_indexing.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "reflengths.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refseq.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "seq.bin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "versionInfo.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "PFAM.domtblout.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusiongdb2.db:md5,9dec75bfc146f56b36e5a84358f9e515",
+                "mitelman.db:md5,c271894c1cd62ba2735a060411c16dea",
+                "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
+                "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
+                "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
+                "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
+                "ctable.bin:md5,12fa024c2df8c586717b84d1b5ef1a1d",
+                "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
+                "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
+                "info.json:md5,d91d8a954afcb4be9fe7bfb3e84b5be6",
+                "mphf.bin:md5,b364192c6c73de4c38ec2ed0750f5155",
+                "pos.bin:md5,dc538263bf4406f72e86d05b5d47ff40",
+                "pre_indexing.log:md5,9f236b9c5a05e8d07b55998fa13d5142",
+                "rank.bin:md5,3fe9c2dc9da7faff6362510c4f29e87d",
+                "refAccumLengths.bin:md5,26d9405e7591ec9a606caa36a094941a",
+                "ref_indexing.log:md5,cbf1d17fa41fb78b8ab8bb39ee3ce191",
+                "reflengths.bin:md5,187a4a0dee316c5dbf440fa3c58d9eec",
+                "refseq.bin:md5,712c4a8d988c15016ea746db7022e549",
+                "seq.bin:md5,7a3803aeb8c8868a8165f1220a79fd95",
+                "versionInfo.json:md5,d2c799050e81aa6e282ac8a73e773941",
+                "Genome:md5,1621486e3b854fefa37c0b4e77275233",
+                "Log.out:md5,ea6563fa0da568e2f239cb747f36b639",
+                "SA:md5,9b6b936f3a8a1232c4fc5533daf9401d",
+                "SAindex:md5,8b2025227b78fdc83e3e5b17c0f538f7",
+                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "genomeParameters.txt:md5,15d295386ecac0ebad47f2c795fb2eb9",
+                "sjdbInfo.txt:md5,f044cb1cd38af66c832f3afc4b1655cb",
+                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+                "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
+                "PFAM.domtblout.dat.gz:md5,36549c1e2826d42aa523d8ce767bbbc7",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -256,65 +254,65 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cancer_splicing.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusion_annot_lib.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "refGene.sort.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                "refGene.sort.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.dat.gz:md5,fb979f3c0bce324096c2d1cbc3031e17",
+                "blast_pairs.idx:md5,18213dbad9fd43ee626b1414d989f3cd",
+                "cancer_splicing.idx:md5,8e8d1b40155fdb8a34702db022c47018",
+                "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                "fusion_annot_lib.idx:md5,6db6da6f0e8cf552d19b53871da647a9",
+                "pfam_domains.dbm:md5,03d53e1bc5529611740796e661da374c",
+                "refGene.bed:md5,6b8a918202faa92c998bdee519e543e1",
+                "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
+                "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
+                "ref_annot.cdna.fa:md5,7f9edf32c6e435a310bc3c88b4920823",
+                "ref_annot.cdna.fa.idx:md5,0d600c7b36feecaf667f2e3f1ba96b5c",
+                "ref_annot.cds:md5,dedb3c22e80db760679fa5e0892e645a",
+                "ref_annot.cdsplus.fa:md5,63623fce6277d627d7b26bcd65d802bf",
+                "ref_annot.cdsplus.fa.idx:md5,89985fa2fa8b613edc21b169fb0b31e3",
+                "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                "ref_annot.gtf.gene_spans:md5,a6cb3cf9858b0f0e2d812571c88cca87",
+                "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+                "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                "ref_annot.pep:md5,5f761d0d491a610f7af3d680765adb48",
+                "ref_annot.prot_info.dbm:md5,a8a6473901b8c741528905d1cf011088",
+                "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+                "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+                "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+                "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                "ref_genome.fa.nin:md5,49f890e1695c7ca57bff748e43e055d7",
+                "ref_genome.fa.njs:md5,9a9b821c184344fee90c293c92fd9f83",
+                "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+                "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+                "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+                "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+                "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                "Log.out:md5,b5185e22316a98556e8ccf17842e5617",
+                "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+                "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "trans.blast.dat.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "HGNC-DB-timestamp.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "hgnc_complete_set.txt:md5,d41d8cd98f00b204e9800998ecf8427e"
+                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "genomeParameters.txt:md5,dd0c3aa259262becaa24347e83193a8d",
+                "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+                "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                "trans.blast.align_coords.align_coords.dbm:md5,f88c5f709a54a68b2965374929e7b1fa",
+                "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
+                "HGNC-DB-timestamp.txt:md5,52f44f480642d69fcc061d48b1b92e6f",
+                "hgnc_complete_set.txt:md5,f90a6d8550e8ebfa9354bf549e0c76d2"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T13:55:10.011645035"
+        "timestamp": "2025-07-29T12:21:16.012319428"
     }
-}
+}
\ No newline at end of file

From a684b1c7b889b70ab34b9c1ff6dd63c7a075ebfe Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 29 Jul 2025 19:17:02 +0000
Subject: [PATCH 720/887] test: fix fusionreport stub test

---
 tests/test_stub_bam.nf.test | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
index cea94105e..b819f3586 100644
--- a/tests/test_stub_bam.nf.test
+++ b/tests/test_stub_bam.nf.test
@@ -211,6 +211,7 @@ nextflow_pipeline {
     }
 
     test("stub test fusionreport") {
+        tag "fusionreport"
 
         when {
             params {
@@ -219,6 +220,7 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 no_cosmic        = true
+                pfam_file        = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
             }
         }
 

From 6d3e9a24b30ed47ddcffaae5814c1988ae843abb Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 30 Jul 2025 13:48:34 +0200
Subject: [PATCH 721/887] fix build snap

---
 tests/test_build.nf.test.snap | 58 +++++++++++++++++------------------
 1 file changed, 29 insertions(+), 29 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 4c35f5e92..92ea46e28 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -190,7 +190,7 @@
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "DB-timestamp.txt:md5,47763915c2614cf9190457bf6916a677",
+                "DB-timestamp.txt:md5,066352a1e399a9b99720ce874d928d36",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusiongdb2.db:md5,9dec75bfc146f56b36e5a84358f9e515",
                 "mitelman.db:md5,c271894c1cd62ba2735a060411c16dea",
@@ -199,22 +199,22 @@
                 "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
-                "ctable.bin:md5,12fa024c2df8c586717b84d1b5ef1a1d",
+                "ctable.bin:md5,62a7501e465b4b9bd72d5793ef6f4b62",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
                 "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
                 "info.json:md5,d91d8a954afcb4be9fe7bfb3e84b5be6",
                 "mphf.bin:md5,b364192c6c73de4c38ec2ed0750f5155",
-                "pos.bin:md5,dc538263bf4406f72e86d05b5d47ff40",
-                "pre_indexing.log:md5,9f236b9c5a05e8d07b55998fa13d5142",
+                "pos.bin:md5,be362bff97c0818bf52a544364ce667d",
+                "pre_indexing.log:md5,4fcc968192564507674c6b6bbd214e4f",
                 "rank.bin:md5,3fe9c2dc9da7faff6362510c4f29e87d",
                 "refAccumLengths.bin:md5,26d9405e7591ec9a606caa36a094941a",
-                "ref_indexing.log:md5,cbf1d17fa41fb78b8ab8bb39ee3ce191",
+                "ref_indexing.log:md5,0ad18446676031eaee9fe5f407b98979",
                 "reflengths.bin:md5,187a4a0dee316c5dbf440fa3c58d9eec",
                 "refseq.bin:md5,712c4a8d988c15016ea746db7022e549",
-                "seq.bin:md5,7a3803aeb8c8868a8165f1220a79fd95",
+                "seq.bin:md5,1b919089152411af82b026b4ea5e33b6",
                 "versionInfo.json:md5,d2c799050e81aa6e282ac8a73e773941",
                 "Genome:md5,1621486e3b854fefa37c0b4e77275233",
-                "Log.out:md5,ea6563fa0da568e2f239cb747f36b639",
+                "Log.out:md5,44beaba8663abcd55654deef3a429c05",
                 "SA:md5,9b6b936f3a8a1232c4fc5533daf9401d",
                 "SAindex:md5,8b2025227b78fdc83e3e5b17c0f538f7",
                 "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
@@ -230,7 +230,7 @@
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-                "PFAM.domtblout.dat.gz:md5,36549c1e2826d42aa523d8ce767bbbc7",
+                "PFAM.domtblout.dat.gz:md5,a8e65e9b2f819124402c43c43008e407",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -254,39 +254,39 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.dat.gz:md5,fb979f3c0bce324096c2d1cbc3031e17",
-                "blast_pairs.idx:md5,18213dbad9fd43ee626b1414d989f3cd",
-                "cancer_splicing.idx:md5,8e8d1b40155fdb8a34702db022c47018",
+                "blast_pairs.dat.gz:md5,a23614ddf03c273a673c552bcea5f42a",
+                "blast_pairs.idx:md5,4371fccf3c269d815544ddbe0aad1475",
+                "cancer_splicing.idx:md5,617eb3ebb3ba78c9eb41b94933d7b22a",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                "fusion_annot_lib.idx:md5,6db6da6f0e8cf552d19b53871da647a9",
-                "pfam_domains.dbm:md5,03d53e1bc5529611740796e661da374c",
-                "refGene.bed:md5,6b8a918202faa92c998bdee519e543e1",
+                "fusion_annot_lib.idx:md5,fe444b9cec7b4ed125f8a29a734f41eb",
+                "pfam_domains.dbm:md5,d7e72c5e499f9dc60531ed58640349ca",
+                "refGene.bed:md5,281400ffc88f3a5d6e98b85dc432eb13",
                 "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
                 "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
-                "ref_annot.cdna.fa:md5,7f9edf32c6e435a310bc3c88b4920823",
-                "ref_annot.cdna.fa.idx:md5,0d600c7b36feecaf667f2e3f1ba96b5c",
-                "ref_annot.cds:md5,dedb3c22e80db760679fa5e0892e645a",
-                "ref_annot.cdsplus.fa:md5,63623fce6277d627d7b26bcd65d802bf",
-                "ref_annot.cdsplus.fa.idx:md5,89985fa2fa8b613edc21b169fb0b31e3",
+                "ref_annot.cdna.fa:md5,cc3b2d0e19c4005b810400f74a6ed252",
+                "ref_annot.cdna.fa.idx:md5,da46bb8791757207b25841eb3e4d6ef4",
+                "ref_annot.cds:md5,7a3f9fafe78c716a77e11efd483aef44",
+                "ref_annot.cdsplus.fa:md5,feb472b2d6eff2c8326d4b5006dcd4d8",
+                "ref_annot.cdsplus.fa.idx:md5,bf390b50ea1d3ee64d5a397e50be55ad",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                "ref_annot.gtf.gene_spans:md5,a6cb3cf9858b0f0e2d812571c88cca87",
+                "ref_annot.gtf.gene_spans:md5,f7852a48c1781233a9a6ecb61e029e83",
                 "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                 "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                "ref_annot.pep:md5,5f761d0d491a610f7af3d680765adb48",
-                "ref_annot.prot_info.dbm:md5,a8a6473901b8c741528905d1cf011088",
+                "ref_annot.pep:md5,9a79dc005d353aeae1bab31016ee1094",
+                "ref_annot.prot_info.dbm:md5,ab2b23b686794ba795f7bee953389c80",
                 "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                 "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                 "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                 "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                "ref_genome.fa.nin:md5,49f890e1695c7ca57bff748e43e055d7",
-                "ref_genome.fa.njs:md5,9a9b821c184344fee90c293c92fd9f83",
+                "ref_genome.fa.nin:md5,685759310f027443c0464ee8454cd177",
+                "ref_genome.fa.njs:md5,a6d1a20f33371d5b3f4f2ed3bd366198",
                 "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                 "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                 "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                 "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                "Log.out:md5,b5185e22316a98556e8ccf17842e5617",
+                "Log.out:md5,ae9df26c48f0a45d3dd76f3ff3d4333d",
                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -297,15 +297,15 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "genomeParameters.txt:md5,dd0c3aa259262becaa24347e83193a8d",
+                "genomeParameters.txt:md5,961034eefe126c2678afe1ace1837e84",
                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                "trans.blast.align_coords.align_coords.dbm:md5,f88c5f709a54a68b2965374929e7b1fa",
+                "trans.blast.align_coords.align_coords.dbm:md5,5f18371156a1c1699acc3ae42dffa0e4",
                 "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
-                "HGNC-DB-timestamp.txt:md5,52f44f480642d69fcc061d48b1b92e6f",
+                "HGNC-DB-timestamp.txt:md5,ad32ca783f87da239a230d4c07144862",
                 "hgnc_complete_set.txt:md5,f90a6d8550e8ebfa9354bf549e0c76d2"
             ]
         ],
@@ -313,6 +313,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-29T12:21:16.012319428"
+        "timestamp": "2025-07-30T13:06:09.650559997"
     }
 }
\ No newline at end of file

From 2da05e3cc1c71a4f71851a8d4d8d653c87625c48 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 30 Jul 2025 14:18:19 +0000
Subject: [PATCH 722/887] test: fix starfusion test

---
 tests/test_stub_bam.nf.test | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
index b819f3586..a5ef83c0f 100644
--- a/tests/test_stub_bam.nf.test
+++ b/tests/test_stub_bam.nf.test
@@ -146,12 +146,15 @@ nextflow_pipeline {
 
     test("stub test starfusion") {
 
+        tag "starfusion"
+
         when {
             params {
                 outdir           = "$outputDir"
                 tools            = "starfusion"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
+                pfam_file        = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
             }
         }
 

From 64d50f24a1fe3378c3e06260f550ea88d7ff76a0 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 30 Jul 2025 16:51:23 +0200
Subject: [PATCH 723/887] fix .nftignore

---
 tests/.nftignore              | 69 +++++++++++++++++------------------
 tests/test_build.nf.test.snap | 38 ++-----------------
 2 files changed, 37 insertions(+), 70 deletions(-)

diff --git a/tests/.nftignore b/tests/.nftignore
index 7f826a5f1..d94f555fe 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -9,39 +9,36 @@ multiqc/multiqc_plots/{svg,pdf,png}/*.{svg,pdf,png}
 multiqc/multiqc_report.html
 fastqc/*_fastqc.{html,zip}
 pipeline_info/*.{html,json,txt,yml}
-references/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz
-references/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz
-references/ensembl/Homo_sapiens.GRCh38.102.dna.primary_assembly.fa
-references/hgnc/HGNC-DB-timestamp.txt
-references/hgnc/hgnc_complete_set.txt
-references/fusion_report_db/DB-timestamp.txt
-references/fusion_report_db/fusiongdb2.db
-references/fusion_report_db/mitelman.db
-references/salmon/salmon/ctable.bin
-references/salmon/salmon/pos.bin
-references/salmon/salmon/pre_indexing.log
-references/salmon/salmon/ref_indexing.log
-references/salmon/salmon/seq.bin
-references/star/Log.out
-references/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/
-references/starfusion/*_genome_lib_build_dir/PFAM.domtblout.dat.gz
-references/starfusion/*_genome_lib_build_dir/blast_pairs.dat.gz
-references/starfusion/*_genome_lib_build_dir/blast_pairs.idx
-references/starfusion/*_genome_lib_build_dir/fusion_annot_lib.idx
-references/starfusion/*_genome_lib_build_dir/pfam_domains.dbm
-references/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa
-references/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa.idx
-references/starfusion/*_genome_lib_build_dir/ref_annot.cds
-references/starfusion/*_genome_lib_build_dir/ref_annot.cdsplus.fa
-references/starfusion/*_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
-references/starfusion/*_genome_lib_build_dir/ref_annot.gtf.gene_spans
-references/starfusion/*_genome_lib_build_dir/ref_annot.pep
-references/starfusion/*_genome_lib_build_dir/ref_annot.prot_info.dbm
-references/starfusion/*_genome_lib_build_dir/ref_genome.fa.nin
-references/starfusion/*_genome_lib_build_dir/ref_genome.fa.njs
-references/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
-references/starfusion/*_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
-references/star/genomeParameters.txt
-references/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
-references/fusion_report_db/mitelman.db
-references/fusion_report_db/fusiongdb2.db
+references/GRCh38/hgnc/HGNC-DB-timestamp.txt
+references/GRCh38/hgnc/hgnc_complete_set.txt
+references/GRCh38/fusion_report_db/DB-timestamp.txt
+references/GRCh38/fusion_report_db/fusiongdb2.db
+references/GRCh38/fusion_report_db/mitelman.db
+references/GRCh38/gencode_v*/salmon/ctable.bin
+references/GRCh38/gencode_v*/salmon/pos.bin
+references/GRCh38/gencode_v*/salmon/pre_indexing.log
+references/GRCh38/gencode_v*/salmon/ref_indexing.log
+references/GRCh38/gencode_v*/salmon/seq.bin
+references/GRCh38/gencode_v*/star/Log.out
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/PFAM.domtblout.dat.gz
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/blast_pairs.dat.gz
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/blast_pairs.idx
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/fusion_annot_lib.idx
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/pfam_domains.dbm
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa.idx
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cds
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cdsplus.fa
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cdsplus.fa.idx
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.gtf.gene_spans
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.pep
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.prot_info.dbm
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_genome.fa.nin
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_genome.fa.njs
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm
+references/GRCh38/gencode_v*/star/genomeParameters.txt
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt
+references/GRCh38/fusion_report_db/mitelman.db
+references/GRCh38/fusion_report_db/fusiongdb2.db
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 92ea46e28..1980d9db8 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -190,31 +190,22 @@
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "DB-timestamp.txt:md5,066352a1e399a9b99720ce874d928d36",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "fusiongdb2.db:md5,9dec75bfc146f56b36e5a84358f9e515",
-                "mitelman.db:md5,c271894c1cd62ba2735a060411c16dea",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
                 "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
-                "ctable.bin:md5,62a7501e465b4b9bd72d5793ef6f4b62",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
                 "duplicate_clusters.tsv:md5,d30ab58d133298ed9ba51f1ba20ce89c",
                 "info.json:md5,d91d8a954afcb4be9fe7bfb3e84b5be6",
                 "mphf.bin:md5,b364192c6c73de4c38ec2ed0750f5155",
-                "pos.bin:md5,be362bff97c0818bf52a544364ce667d",
-                "pre_indexing.log:md5,4fcc968192564507674c6b6bbd214e4f",
                 "rank.bin:md5,3fe9c2dc9da7faff6362510c4f29e87d",
                 "refAccumLengths.bin:md5,26d9405e7591ec9a606caa36a094941a",
-                "ref_indexing.log:md5,0ad18446676031eaee9fe5f407b98979",
                 "reflengths.bin:md5,187a4a0dee316c5dbf440fa3c58d9eec",
                 "refseq.bin:md5,712c4a8d988c15016ea746db7022e549",
-                "seq.bin:md5,1b919089152411af82b026b4ea5e33b6",
                 "versionInfo.json:md5,d2c799050e81aa6e282ac8a73e773941",
                 "Genome:md5,1621486e3b854fefa37c0b4e77275233",
-                "Log.out:md5,44beaba8663abcd55654deef3a429c05",
                 "SA:md5,9b6b936f3a8a1232c4fc5533daf9401d",
                 "SAindex:md5,8b2025227b78fdc83e3e5b17c0f538f7",
                 "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
@@ -224,13 +215,11 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "genomeParameters.txt:md5,15d295386ecac0ebad47f2c795fb2eb9",
                 "sjdbInfo.txt:md5,f044cb1cd38af66c832f3afc4b1655cb",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-                "PFAM.domtblout.dat.gz:md5,a8e65e9b2f819124402c43c43008e407",
                 "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -254,39 +243,24 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "blast_pairs.dat.gz:md5,a23614ddf03c273a673c552bcea5f42a",
-                "blast_pairs.idx:md5,4371fccf3c269d815544ddbe0aad1475",
-                "cancer_splicing.idx:md5,617eb3ebb3ba78c9eb41b94933d7b22a",
+                "cancer_splicing.idx:md5,b52020541bd3503fe10eadccc9aa375f",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                "fusion_annot_lib.idx:md5,fe444b9cec7b4ed125f8a29a734f41eb",
-                "pfam_domains.dbm:md5,d7e72c5e499f9dc60531ed58640349ca",
-                "refGene.bed:md5,281400ffc88f3a5d6e98b85dc432eb13",
+                "refGene.bed:md5,1496ffb8d112c0842b8de9c7846426da",
                 "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
                 "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
-                "ref_annot.cdna.fa:md5,cc3b2d0e19c4005b810400f74a6ed252",
-                "ref_annot.cdna.fa.idx:md5,da46bb8791757207b25841eb3e4d6ef4",
-                "ref_annot.cds:md5,7a3f9fafe78c716a77e11efd483aef44",
-                "ref_annot.cdsplus.fa:md5,feb472b2d6eff2c8326d4b5006dcd4d8",
-                "ref_annot.cdsplus.fa.idx:md5,bf390b50ea1d3ee64d5a397e50be55ad",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-                "ref_annot.gtf.gene_spans:md5,f7852a48c1781233a9a6ecb61e029e83",
                 "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
                 "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-                "ref_annot.pep:md5,9a79dc005d353aeae1bab31016ee1094",
-                "ref_annot.prot_info.dbm:md5,ab2b23b686794ba795f7bee953389c80",
                 "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
                 "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
                 "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
                 "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-                "ref_genome.fa.nin:md5,685759310f027443c0464ee8454cd177",
-                "ref_genome.fa.njs:md5,a6d1a20f33371d5b3f4f2ed3bd366198",
                 "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
                 "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
                 "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
                 "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
                 "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-                "Log.out:md5,ae9df26c48f0a45d3dd76f3ff3d4333d",
                 "SA:md5,7dd9083264be9c6a2194d990bc10d237",
                 "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
                 "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -297,22 +271,18 @@
                 "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
                 "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
                 "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-                "genomeParameters.txt:md5,961034eefe126c2678afe1ace1837e84",
                 "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
                 "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
                 "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
                 "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
                 "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-                "trans.blast.align_coords.align_coords.dbm:md5,5f18371156a1c1699acc3ae42dffa0e4",
-                "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
-                "HGNC-DB-timestamp.txt:md5,ad32ca783f87da239a230d4c07144862",
-                "hgnc_complete_set.txt:md5,f90a6d8550e8ebfa9354bf549e0c76d2"
+                "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-30T13:06:09.650559997"
+        "timestamp": "2025-07-30T16:50:27.807686458"
     }
 }
\ No newline at end of file

From 54ba946dd47c799b0f9c228d3956dc35e76f471b Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 31 Jul 2025 10:03:47 +0200
Subject: [PATCH 724/887] fix build test again

---
 tests/.nftignore              | 2 ++
 tests/test_build.nf.test.snap | 4 +---
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/tests/.nftignore b/tests/.nftignore
index d94f555fe..da508b609 100644
--- a/tests/.nftignore
+++ b/tests/.nftignore
@@ -20,12 +20,14 @@ references/GRCh38/gencode_v*/salmon/pre_indexing.log
 references/GRCh38/gencode_v*/salmon/ref_indexing.log
 references/GRCh38/gencode_v*/salmon/seq.bin
 references/GRCh38/gencode_v*/star/Log.out
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_genome.fa.star.idx/
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/PFAM.domtblout.dat.gz
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/blast_pairs.dat.gz
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/blast_pairs.idx
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/fusion_annot_lib.idx
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/pfam_domains.dbm
+references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/refGene.bed
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cdna.fa.idx
 references/GRCh38/gencode_v*/starfusion/*_genome_lib_build_dir/ref_annot.cds
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 1980d9db8..571e920b8 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -243,9 +243,7 @@
                 "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "cancer_splicing.idx:md5,b52020541bd3503fe10eadccc9aa375f",
                 "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-                "refGene.bed:md5,1496ffb8d112c0842b8de9c7846426da",
                 "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
                 "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
                 "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
@@ -283,6 +281,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-30T16:50:27.807686458"
+        "timestamp": "2025-07-31T10:02:44.229294202"
     }
 }
\ No newline at end of file

From 3043560e6d340d0aad24f179919271e465ad47bf Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 4 Aug 2025 14:28:51 +0000
Subject: [PATCH 725/887] Update `test_build` to use reduced Pfam and Dfam
 files

---
 conf/test_build.config | 12 ++++++------
 1 file changed, 6 insertions(+), 6 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index 15178782d..ca76db564 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -45,12 +45,12 @@ params {
     species                    = 'homo_sapiens'
     dfam_version               = 3.4
     pfam_version               = 37.4
-    dfam_hmm                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm"
-    dfam_h3f                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3f"
-    dfam_h3i                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3i"
-    dfam_h3m                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3m"
-    dfam_h3p                   = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam.hmm.h3p"
-    pfam_file                  = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A.hmm.gz"
+    dfam_hmm                   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm"
+    dfam_h3f                   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f"
+    dfam_h3i                   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i"
+    dfam_h3m                   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m"
+    dfam_h3p                   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
+    pfam_file                  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
 
 }
 

From 7b0b2d070f625b9a0d15c6f401cde99d13473fc1 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 4 Aug 2025 14:47:31 +0000
Subject: [PATCH 726/887] Small change to trigger test

---
 tests/test_build.nf.test | 1 -
 1 file changed, 1 deletion(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index df43626a9..70e87771c 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -8,7 +8,6 @@ nextflow_pipeline {
     tag "test_build"
 
     test("Test build references") {
-
         when {
             params {
                 outdir = "$outputDir"

From 5f33759b5215f884b5967a250b21b97d2cbe5189 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 4 Aug 2025 14:52:33 +0000
Subject: [PATCH 727/887] Update `CHANGELOG.md`

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 76b8acb58..cf167d00d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -87,6 +87,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Updated arriba to v2.5.0 [#693](https://github.com/nf-core/rnafusion/pull/693)
 - Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
 - Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
+- Modified `test_build` profile to use a reduced version of Pfam and Dfam files [#733](https://github.com/nf-core/rnafusion/pull/733)
 
 ### Fixed
 

From 7f92ad17a5d5e2c34da7b6ef0fa4426eeebd8b56 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Mon, 4 Aug 2025 15:06:24 +0000
Subject: [PATCH 728/887] Update tests for local modules to use reduced Pfam
 and Dfam files

---
 .../ctatsplicing/prepgenomelib/tests/main.nf.test    | 12 ++++++------
 modules/local/fusioninspector/tests/main.nf.test     | 12 ++++++------
 modules/local/starfusion/detect/tests/main.nf.test   | 12 ++++++------
 3 files changed, 18 insertions(+), 18 deletions(-)

diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
index 1923fdd1a..86886fd08 100644
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -20,13 +20,13 @@ nextflow_process {
 
                 input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                 input[3] = "homo_sapiens"
-                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
                 input[5] = [
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
                 ]
                 input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                 """
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/local/fusioninspector/tests/main.nf.test
index e1a42c1b1..fb1374633 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/local/fusioninspector/tests/main.nf.test
@@ -26,13 +26,13 @@ nextflow_process {
 
                     input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                     input[3] = "homo_sapiens"
-                    input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                    input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
                     input[5] = [
-                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
-                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
-                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
-                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
-                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
                     ]
                     input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                     """
diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
index 599d771f3..2dec2d2cf 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test
+++ b/modules/local/starfusion/detect/tests/main.nf.test
@@ -20,13 +20,13 @@ nextflow_process {
 
                 input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                 input[3] = "homo_sapiens"
-                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
                 input[5] = [
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
+                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
                 ]
                 input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                 """

From d20c72774b436310763e982b0863259b46e18757 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Tue, 5 Aug 2025 09:52:34 +0200
Subject: [PATCH 729/887] Update README.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 README.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 3730052bb..8c8b0f845 100644
--- a/README.md
+++ b/README.md
@@ -42,7 +42,7 @@ The path to the downloaded references can then be provided to the pipeline with
 
 :warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**
 
-The pipeline is also able to build the references in case files from a specific gencode version are missing. This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.
+The pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only genocode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.
 
 1. Download gencode fasta and gtf files
 2. Create [STAR](https://github.com/alexdobin/STAR) index

From 5455ae872b967c88e853fca992449aa69913929c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Tue, 5 Aug 2025 09:53:36 +0200
Subject: [PATCH 730/887] Update docs/usage.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index 6be6aa0c3..d854c68a3 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -50,7 +50,7 @@ nextflow run nf-core/rnafusion \
 > **IMPORTANT**
 >
 > - Note that this step takes about 24 hours to complete on HPC.
-> - Do not provide a samplesheet via the `input` parameter, otherwise the pipeline will run the analysis directly after downloading the references (except if that is what you want).
+> 
 
 References for each tools can also be downloaded separately with:
 

From 07f61c986549050998482f700f5e1b899b5ff9aa Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 5 Aug 2025 10:17:06 +0200
Subject: [PATCH 731/887] fix lint issues

---
 docs/usage.md                                        |  1 -
 .../local/utils_nfcore_rnafusion_pipeline/main.nf    | 12 ++++++------
 2 files changed, 6 insertions(+), 7 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index d854c68a3..5c3905b99 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -50,7 +50,6 @@ nextflow run nf-core/rnafusion \
 > **IMPORTANT**
 >
 > - Note that this step takes about 24 hours to complete on HPC.
-> 
 
 References for each tools can also be downloaded separately with:
 
diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
index 5b28ea165..89219ce3d 100644
--- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf
@@ -165,19 +165,19 @@ def validateInputParameters() {
     def dfamParams = ['dfam_hmm', 'dfam_h3p', 'dfam_h3m', 'dfam_h3i', 'dfam_h3f']
 
     if (params.dfam_version) {
-        def dfamPattern = /https:\/\/www\.dfam\.org\/releases\/Dfam_${params.dfam_version}\/infrastructure\/dfamscan\/${params.species}_dfam\..*/
+        def dfamPattern = "https://www.dfam.org/releases/Dfam_${params.dfam_version}/infrastructure/dfamscan/${params.species}_dfam"
 
         def setDfamParams = dfamParams.findAll { params[it] }
 
         if (setDfamParams) {
             def customParams = setDfamParams.findAll { paramName ->
-                !params[paramName].matches(dfamPattern)
+                !params[paramName]?.startsWith(dfamPattern)
             }
             if (customParams) {
                 def paramDetails = customParams.collect { paramName ->
                     "   --${paramName}: ${params[paramName]}"
                 }.join('\n')
-                dfam_warn = "Both custom DFAM paths as well as `--dfam_version` (${params.dfam_version}) and `--species` (${params.species}) were provided.\n" +
+                def dfam_warn = "Both custom DFAM paths as well as `--dfam_version` (${params.dfam_version}) and `--species` (${params.species}) were provided.\n" +
                     "Custom DFAM paths parameters provided:\n${paramDetails}\n" +
                     "The pipeline will prioritize these custom files specified with `--${customParams}` and **will NOT** construct these URLs based on `--dfam_version` nor `--species`.\n" +
                     "   - If you intend to use custom DFAM files, please ensure that all `--dfam_h*` parameters point to full and valid paths.\n" +
@@ -188,10 +188,10 @@ def validateInputParameters() {
     }
 
     if (params.pfam_version){
-        def pfamPattern = /http:\/\/ftp\.ebi\.ac\.uk\/pub\/databases\/Pfam\/releases\/Pfam${params.pfam_version}\/Pfam-A\..*/
+        def pfamPattern = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam${params.pfam_version}/Pfam-A"
 
-        if (!(params.pfam_file.matches(pfamPattern))){
-            pfam_warn = "Both `--pfam_file` (${params.pfam_file}) and `--pfam_version` (${params.pfam_version}) were provided.\n" +
+        if (!(params.pfam_file?.startsWith(pfamPattern))) {
+            def pfam_warn = "Both `--pfam_file` (${params.pfam_file}) and `--pfam_version` (${params.pfam_version}) were provided.\n" +
                     "The pipeline will prioritize the custom file from `--pfam_file` and **will NOT** construct the URL based on `--pfam_version`.\n" +
                     "   - If you intend to use a custom PFAM file, please ensure that `--pfam_file` points to a full and valid path.\n" +
                     "   - If you prefer to let the pipeline build the PFAM URL automatically, omit `--pfam_file` and instead provide only `--pfam_version`."

From 3f5ca6e14ee385c5bc5e6643df5c47503361046d Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 5 Aug 2025 10:20:31 +0200
Subject: [PATCH 732/887] fix nf-core lint

---
 ro-crate-metadata.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 50b780538..2d97d3a9e 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing. This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only genocode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"

From e1aaa6885cd98b01eca53db316c7796c1a52e57c Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 5 Aug 2025 10:39:28 +0200
Subject: [PATCH 733/887] improve non-buildable references handling

---
 conf/test.config            | 10 +++----
 main.nf                     | 19 ++++++++++++
 nextflow_schema.json        |  5 ----
 tests/test_stub.nf.test     | 58 +++++++++++++++++++++++++++++++++++--
 tests/test_stub_bam.nf.test | 52 +++++++++++++++++++++++++++++++--
 5 files changed, 129 insertions(+), 15 deletions(-)

diff --git a/conf/test.config b/conf/test.config
index 13c8c62e7..9cfdbf5de 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -19,11 +19,11 @@ params {
     tools                       = "all"
     no_cosmic                   = true
     genomes_base                = "${params.outdir}/references"
-    arriba_ref_protein_domains  = null
-    arriba_ref_known_fusions    = null
-    arriba_ref_blacklist        = null
-    arriba_ref_cytobands        = null
-    fusioncatcher_ref           = null
+    arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+    arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+    fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
 }
 
 // Limit and standardize resources for github actions and reproducibility
diff --git a/main.nf b/main.nf
index 8561d70db..56a8fcf9e 100644
--- a/main.nf
+++ b/main.nf
@@ -79,6 +79,25 @@ workflow {
         error("Conda or Mamba runs are not supported when ctatsplicing is in `--tools`")
     }
 
+    if (tools.contains("fusioncatcher") && (!params.fusioncatcher_ref || !file(params.fusioncatcher_ref).exists())) {
+        error("You have selected `fusioncatcher` in `--tools`, but did not provide an existing path to the fusioncatcher reference files with `--fusioncatcher_ref`.")
+    }
+
+    if (tools.contains("arriba")) {
+        if (!params.arriba_ref_blacklist || !file(params.arriba_ref_blacklist).exists()) {
+            error("You have selected `arriba` in `--tools`, but did not provide an existing path to the arriba reference blacklist file with `--arriba_ref_blacklist`.")
+        }
+        if (!params.arriba_ref_cytobands || !file(params.arriba_ref_cytobands).exists()) {
+            error("You have selected `arriba` in `--tools`, but did not provide an existing path to the arriba reference cytobands file with `--arriba_ref_cytobands`.")
+        }
+        if (!params.arriba_ref_known_fusions || !file(params.arriba_ref_known_fusions).exists()) {
+            error("You have selected `arriba` in `--tools`, but did not provide an existing path to the arriba reference known fusions file with `--arriba_ref_known_fusions`.")
+        }
+        if (!params.arriba_ref_protein_domains || !file(params.arriba_ref_protein_domains).exists()) {
+            error("You have selected `arriba` in `--tools`, but did not provide an existing path to the arriba reference protein domains file with `--arriba_ref_protein_domains`.")
+        }
+    }
+
     //
     // WORKFLOW: Run main workflow
     //
diff --git a/nextflow_schema.json b/nextflow_schema.json
index eb6ce4b1b..3c5cf1498 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -102,7 +102,6 @@
                 "arriba_ref_blacklist": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.tsv(\\.gz)?$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference blacklist"
@@ -110,7 +109,6 @@
                 "arriba_ref_cytobands": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.tsv$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference cytobands"
@@ -118,7 +116,6 @@
                 "arriba_ref_known_fusions": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.tsv(\\.gz)?$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference known fusions"
@@ -126,7 +123,6 @@
                 "arriba_ref_protein_domains": {
                     "type": "string",
                     "format": "file-path",
-                    "exists": true,
                     "pattern": "\\S+\\.gff3$",
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba reference protein domain"
@@ -162,7 +158,6 @@
                 "fusioncatcher_ref": {
                     "type": "string",
                     "format": "directory-path",
-                    "exists": true,
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
diff --git a/tests/test_stub.nf.test b/tests/test_stub.nf.test
index f31b605e1..08492ea92 100644
--- a/tests/test_stub.nf.test
+++ b/tests/test_stub.nf.test
@@ -17,6 +17,11 @@ nextflow_pipeline {
                 tools            = "all"
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -50,7 +55,11 @@ nextflow_pipeline {
                 tools            = "arriba"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
-
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -84,7 +93,11 @@ nextflow_pipeline {
                 tools            = "ctatsplicing"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
-
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -118,6 +131,11 @@ nextflow_pipeline {
                 tools            = "fusioncatcher"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -152,7 +170,11 @@ nextflow_pipeline {
                 trim_tail_fusioncatcher = 50
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
-
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
         then {
@@ -185,6 +207,11 @@ nextflow_pipeline {
                 tools            = "starfusion"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -218,6 +245,11 @@ nextflow_pipeline {
                 tools            = "stringtie"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -252,6 +284,11 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -285,6 +322,11 @@ nextflow_pipeline {
                 tools            = "fastp"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -318,6 +360,11 @@ nextflow_pipeline {
                 tools            = "salmon"
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -352,6 +399,11 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
index 9d7907b75..46c06d9df 100644
--- a/tests/test_stub_bam.nf.test
+++ b/tests/test_stub_bam.nf.test
@@ -18,7 +18,11 @@ nextflow_pipeline {
                 tools            = "all"
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
-
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -54,7 +58,11 @@ nextflow_pipeline {
                 input            = "${projectDir}/tests/yml/bam.yml"
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
-
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -89,6 +97,11 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -123,6 +136,11 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -160,6 +178,11 @@ nextflow_pipeline {
                 input            = "${projectDir}/tests/yml/bam.yml"
                 genomes_base     = "${outputDir}/references"
                 pfam_file        = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -194,6 +217,11 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -231,6 +259,11 @@ nextflow_pipeline {
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
                 pfam_file        = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -265,6 +298,11 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -299,6 +337,11 @@ nextflow_pipeline {
                 fusion_annot_lib = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz'
                 input            = "${projectDir}/tests/yml/bam.yml"
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 
@@ -334,6 +377,11 @@ nextflow_pipeline {
                 input            = "${projectDir}/tests/yml/bam.yml"
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
+                arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref           = "${projectDir}/assets" // This is a placeholder until a small reference can be created
             }
         }
 

From 0da9176be6c5a511defd34ae549978bca526d70c Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 5 Aug 2025 14:20:42 +0000
Subject: [PATCH 734/887] feat: add rrnatranscripts from nf-core

---
 .../nf-core/rrnatranscripts/environment.yml   |  7 ++
 modules/nf-core/rrnatranscripts/main.nf       | 34 +++++++++
 modules/nf-core/rrnatranscripts/meta.yml      | 50 +++++++++++++
 .../templates/get_rrna_transcripts.py         | 71 ++++++++++++++++++
 .../rrnatranscripts/tests/main.nf.test        | 55 ++++++++++++++
 .../rrnatranscripts/tests/main.nf.test.snap   | 72 +++++++++++++++++++
 6 files changed, 289 insertions(+)
 create mode 100644 modules/nf-core/rrnatranscripts/environment.yml
 create mode 100644 modules/nf-core/rrnatranscripts/main.nf
 create mode 100644 modules/nf-core/rrnatranscripts/meta.yml
 create mode 100644 modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
 create mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test
 create mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test.snap

diff --git a/modules/nf-core/rrnatranscripts/environment.yml b/modules/nf-core/rrnatranscripts/environment.yml
new file mode 100644
index 000000000..6f09494b4
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - python=3.12.2
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
new file mode 100644
index 000000000..67fa29b09
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/main.nf
@@ -0,0 +1,34 @@
+process RRNATRANSCRIPTS {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/python:3.12' :
+        'biocontainers/python:3.12' }"
+
+    input:
+    tuple val(meta), path(gtf)
+
+    output:
+    tuple val(meta), path('*_rrna_intervals.gtf')    , emit: rrna_gtf
+    path "versions.yml"                              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    template 'get_rrna_transcripts.py'
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}_rrna_intervals.gtf
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        python: \$(python --version | sed -e "s/Python //g")
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/rrnatranscripts/meta.yml b/modules/nf-core/rrnatranscripts/meta.yml
new file mode 100644
index 000000000..f793fbdd9
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/meta.yml
@@ -0,0 +1,50 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: rrnatranscripts
+description: Ribosomal RNA extraction from a GTF file.
+keywords:
+  - ribosomal
+  - rna
+  - genomics
+tools:
+  - rrnatranscripts:
+      description: |
+        Extraction of ribosomal RNA
+      homepage: https://github.com/nf-core/rnafusion
+      licence: ["GPL-3.0-or-later"]
+      identifier: ""
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - gtf:
+        type: file
+        description: GTF file
+        pattern: "*.{gtf}"
+        ontologies:
+          - edam: "http://edamontology.org/format_2306" # GTF
+output:
+  rrna_gtf:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*_rrna_intervals.gtf":
+          type: file
+          description: Output GTF file containing ribosomal RNA intervals
+          pattern: "*_rrna_intervals.gtf"
+          ontologies:
+            - edam: "http://edamontology.org/format_2306" # GTF
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: "http://edamontology.org/format_3750" # YAML
+authors:
+  - "@rannick"
+maintainers:
+  - "@rannick"
diff --git a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
new file mode 100644
index 000000000..b01c7d902
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
@@ -0,0 +1,71 @@
+#!/usr/bin/env python3
+
+"""
+Author:
+    Annick Renevey
+
+Copyright (c) 2025, Annick Renevey. All rights reserved.
+
+License: GPL-3 License
+"""
+
+import platform
+from pathlib import Path
+
+def format_yaml_like(data: dict, indent: int = 0) -> str:
+    """Formats a dictionary to a YAML-like string.
+
+    Args:
+        data (dict): The dictionary to format.
+        indent (int): The current indentation level.
+
+    Returns:
+        str: A string formatted as YAML.
+    """
+    yaml_str = ""
+    for key, value in data.items():
+        spaces = "  " * indent
+        if isinstance(value, dict):
+            yaml_str += f"{spaces}{key}:\\n{format_yaml_like(value, indent + 1)}"
+        else:
+            yaml_str += f"{spaces}{key}: {value}\\n"
+    return yaml_str
+
+def get_rrna_intervals(file_in: Path, file_out: Path):
+    """
+    Extracts rRNA lines from a GTF and writes them out.
+    """
+    patterns = {
+        "#",
+        'transcript_biotype "Mt_rRNA"',
+        'transcript_biotype "rRNA"',
+        'transcript_biotype "rRNA_pseudogene"',
+    }
+    line_starts = {"MT", "1", "2", "3", "4", "5", "6", "7", "8", "9"}
+    out_lines = []
+
+    with file_in.open() as f:
+        for line in f:
+            if any(p in line for p in patterns) and any(line.startswith(ls) for ls in line_starts):
+                out_lines.append(line)
+
+    file_out.parent.mkdir(parents=True, exist_ok=True)
+    with file_out.open("w") as out:
+        out.writelines(out_lines)
+
+# Main
+gtf_path  = Path("${gtf}")
+prefix    = "${prefix}"
+out_gtf   = Path(f"{prefix}_rrna_intervals.gtf")
+
+get_rrna_intervals(gtf_path, out_gtf)
+
+# Versions
+versions = {
+    "${task.process}": {
+        "python": platform.python_version()
+    }
+}
+
+with open("versions.yml", "w") as v:
+    v.write(format_yaml_like(versions))
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test b/modules/nf-core/rrnatranscripts/tests/main.nf.test
new file mode 100644
index 000000000..46ef3d048
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/tests/main.nf.test
@@ -0,0 +1,55 @@
+nextflow_process {
+
+    name "Test Process RRNATRANSCRIPTS"
+    script "../main.nf"
+    process "RRNATRANSCRIPTS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "rrnatranscripts"
+
+    test("homo_sapiens - gtf") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id: 'test', single_end: false ],  // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/riboseq_expression/Homo_sapiens.GRCh38.111_chr20.gtf', checkIfExists: true)
+                ]                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - gtf - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id: 'test', single_end: false ],  // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
+                ]                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap b/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
new file mode 100644
index 000000000..56b1f0955
--- /dev/null
+++ b/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
@@ -0,0 +1,72 @@
+{
+    "homo_sapiens - gtf": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_rrna_intervals.gtf:md5,db5d8f2c3cdd3135aeb1d6081e866caa"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,c1ef051fc08870f863ad611865f3f350"
+                ],
+                "rrna_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_rrna_intervals.gtf:md5,db5d8f2c3cdd3135aeb1d6081e866caa"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c1ef051fc08870f863ad611865f3f350"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-01T15:25:06.491928779"
+    },
+    "homo_sapiens - gtf - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
+                ],
+                "rrna_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-28T14:27:37.339133522"
+    }
+}
\ No newline at end of file

From da9f104716b2d0427388d98ded56e7825e579210 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 5 Aug 2025 14:21:25 +0000
Subject: [PATCH 735/887] feat: add agat/convertgff2bed from nf-core

---
 modules.json                                  | 10 +++
 .../agat/convertgff2bed/environment.yml       |  5 ++
 modules/nf-core/agat/convertgff2bed/main.nf   | 46 ++++++++++++
 modules/nf-core/agat/convertgff2bed/meta.yml  | 57 +++++++++++++++
 .../agat/convertgff2bed/tests/main.nf.test    | 58 +++++++++++++++
 .../convertgff2bed/tests/main.nf.test.snap    | 72 +++++++++++++++++++
 .../agat/convertgff2bed/tests/nextflow.config |  5 ++
 7 files changed, 253 insertions(+)
 create mode 100644 modules/nf-core/agat/convertgff2bed/environment.yml
 create mode 100644 modules/nf-core/agat/convertgff2bed/main.nf
 create mode 100644 modules/nf-core/agat/convertgff2bed/meta.yml
 create mode 100644 modules/nf-core/agat/convertgff2bed/tests/main.nf.test
 create mode 100644 modules/nf-core/agat/convertgff2bed/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/agat/convertgff2bed/tests/nextflow.config

diff --git a/modules.json b/modules.json
index f69fb565c..972c32a9a 100644
--- a/modules.json
+++ b/modules.json
@@ -5,6 +5,11 @@
         "https://github.com/nf-core/modules.git": {
             "modules": {
                 "nf-core": {
+                    "agat/convertgff2bed": {
+                        "branch": "master",
+                        "git_sha": "b785bb0b56bb518ab40c41f41d0e8749fb638257",
+                        "installed_by": ["modules"]
+                    },
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
@@ -95,6 +100,11 @@
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
                         "installed_by": ["modules"]
                     },
+                    "rrnatranscripts": {
+                        "branch": "master",
+                        "git_sha": "b785bb0b56bb518ab40c41f41d0e8749fb638257",
+                        "installed_by": ["modules"]
+                    },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
diff --git a/modules/nf-core/agat/convertgff2bed/environment.yml b/modules/nf-core/agat/convertgff2bed/environment.yml
new file mode 100644
index 000000000..0e342bfda
--- /dev/null
+++ b/modules/nf-core/agat/convertgff2bed/environment.yml
@@ -0,0 +1,5 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - "bioconda::agat=1.5.1"
diff --git a/modules/nf-core/agat/convertgff2bed/main.nf b/modules/nf-core/agat/convertgff2bed/main.nf
new file mode 100644
index 000000000..39ed1f617
--- /dev/null
+++ b/modules/nf-core/agat/convertgff2bed/main.nf
@@ -0,0 +1,46 @@
+process AGAT_CONVERTGFF2BED {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/03/033434db0bd6ba28660401e1059286f36641fd8ce55faa11973fe5eaf312adcd/data' :
+        'community.wave.seqera.io/library/agat:1.5.1--ae3cd948ce5e9795' }"
+
+    input:
+    tuple val(meta), path(gff)
+
+    output:
+    tuple val(meta), path("*.bed")      , emit: bed
+    path "versions.yml"                 , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args   = task.ext.args   ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    agat_convert_sp_gff2bed.pl \\
+        --gff ${gff} \\
+        -o ${prefix}.bed \\
+        ${args}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        agat: \$(agat_convert_sp_gff2bed.pl --help | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p')
+    END_VERSIONS
+    """
+
+    stub:
+    def args   = task.ext.args   ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.bed
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        agat: \$(agat_convert_sp_gff2bed.pl --help | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/agat/convertgff2bed/meta.yml b/modules/nf-core/agat/convertgff2bed/meta.yml
new file mode 100644
index 000000000..1e1108f53
--- /dev/null
+++ b/modules/nf-core/agat/convertgff2bed/meta.yml
@@ -0,0 +1,57 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "agat_convertgff2bed"
+description: Takes a GFF3 file and converts to a bed12 file
+keywords:
+  - genome
+  - bed
+  - gff
+  - conversion
+tools:
+  - "agat":
+      description: "Another Gff Analysis Toolkit (AGAT). Suite of tools to handle gene
+        annotations in any GTF/GFF format."
+      homepage: "https://agat.readthedocs.io/en/latest/"
+      documentation: "https://agat.readthedocs.io/en/latest/"
+      tool_dev_url: "https://github.com/NBISweden/AGAT"
+      doi: "10.5281/zenodo.3552717"
+      licence: ["GPL v3"]
+      identifier: biotools:AGAT
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1' ]`
+    - gff:
+        type: file
+        description: Input GFF3 file
+        pattern: "*.{gff}"
+        ontologies:
+          - edam: "http://edamontology.org/format_1975" # GFF3
+
+output:
+  bed:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1' ]`
+      - "*.bed":
+          type: file
+          description: Output bed12 file
+          pattern: "*.bed"
+          ontologies:
+            - edam: "http://edamontology.org/format_3586" # bed12
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: "http://edamontology.org/format_3750" # YAML
+
+authors:
+  - "@atrigila"
+maintainers:
+  - "@atrigila"
diff --git a/modules/nf-core/agat/convertgff2bed/tests/main.nf.test b/modules/nf-core/agat/convertgff2bed/tests/main.nf.test
new file mode 100644
index 000000000..70402a803
--- /dev/null
+++ b/modules/nf-core/agat/convertgff2bed/tests/main.nf.test
@@ -0,0 +1,58 @@
+nextflow_process {
+
+    name "Test Process AGAT_CONVERTGFF2BED"
+    script "../main.nf"
+    process "AGAT_CONVERTGFF2BED"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "agat"
+    tag "agat/convertgff2bed"
+
+    test("sarscov2 genome - gff3") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+    test("sarscov2 genome - gff3 - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/sarscov2/genome/genome.gff3", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/agat/convertgff2bed/tests/main.nf.test.snap b/modules/nf-core/agat/convertgff2bed/tests/main.nf.test.snap
new file mode 100644
index 000000000..455a78c7c
--- /dev/null
+++ b/modules/nf-core/agat/convertgff2bed/tests/main.nf.test.snap
@@ -0,0 +1,72 @@
+{
+    "sarscov2 genome - gff3": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bed:md5,d424ee1b9d9982f129412f491fca709e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,7e74ce85271b5cc6b522c049c3902f4f"
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bed:md5,d424ee1b9d9982f129412f491fca709e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,7e74ce85271b5cc6b522c049c3902f4f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-01T15:44:10.214333104"
+    },
+    "sarscov2 genome - gff3 - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,7e74ce85271b5cc6b522c049c3902f4f"
+                ],
+                "bed": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,7e74ce85271b5cc6b522c049c3902f4f"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-01T15:44:21.236419314"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/agat/convertgff2bed/tests/nextflow.config b/modules/nf-core/agat/convertgff2bed/tests/nextflow.config
new file mode 100644
index 000000000..b465c0ea0
--- /dev/null
+++ b/modules/nf-core/agat/convertgff2bed/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: CONVERTGFF2BED {
+        ext.args = "-f gene,mRNA,exon,CDS,five_prime_UTR,three_prime_UTR"
+    }
+}

From 9cc949f7ac35fd44c395072f5332df3c9ca92eba Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 5 Aug 2025 14:22:04 +0000
Subject: [PATCH 736/887] feat: replace local rrnatranscripts for nf-core
 versions

---
 subworkflows/local/build_references/main.nf | 16 +++++++++++-----
 1 file changed, 11 insertions(+), 5 deletions(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 2ac9141f6..f14f70135 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -8,7 +8,6 @@ include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode
 include { FUSIONCATCHER_DOWNLOAD }          from '../../../modules/local/fusioncatcher/download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
 include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
-include { GET_RRNA_TRANSCRIPTS }            from '../../../modules/local/get_rrna_transcript/main'
 include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatsplicing/prepgenomelib/main.nf'
 
 /*
@@ -16,7 +15,8 @@ include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatspl
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
-
+include { RRNATRANSCRIPTS                 } from '../../../modules/nf-core/rrnatranscripts/main'
+include { AGAT_CONVERTGFF2BED             } from '../../../modules/nf-core/agat/convertgff2bed/main'
 include { ARRIBA_DOWNLOAD }                 from '../../../modules/nf-core/arriba/download/main'
 include { SAMTOOLS_FAIDX }                  from '../../../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../../../modules/nf-core/star/genomegenerate/main'
@@ -84,10 +84,16 @@ workflow BUILD_REFERENCES {
         if (!exists_not_empty(params.rrna_intervals)){
             GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
             ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
-            GET_RRNA_TRANSCRIPTS(ch_gtf)
-            ch_versions = ch_versions.mix(GET_RRNA_TRANSCRIPTS.out.versions)
-            GATK4_BEDTOINTERVALLIST(GET_RRNA_TRANSCRIPTS.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
+
+            RRNATRANSCRIPTS(ch_gtf)
+            ch_versions = ch_versions.mix(RRNATRANSCRIPTS.out.versions)
+
+            AGAT_CONVERTGFF2BED(RRNATRANSCRIPTS.out.rrna_gtf)
+            ch_versions = ch_versions.mix(AGAT_CONVERTGFF2BED.out.versions)
+
+            GATK4_BEDTOINTERVALLIST(AGAT_CONVERTGFF2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict )
             ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions)
+
             ch_rrna_interval = GATK4_BEDTOINTERVALLIST.out.interval_list
         } else {
             ch_rrna_interval = Channel.fromPath(params.rrna_intervals).map { that -> [[id:that.Name], that] }

From bb19240a96d6086dd814ea6c2c85dc62bac3f52f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 5 Aug 2025 15:06:38 +0000
Subject: [PATCH 737/887] feat: add bioawk module

---
 conf/modules.config                           | 11 ++++
 modules.json                                  |  5 ++
 modules/nf-core/bioawk/environment.yml        |  7 +++
 modules/nf-core/bioawk/main.nf                | 38 ++++++++++++
 modules/nf-core/bioawk/meta.yml               | 58 +++++++++++++++++++
 modules/nf-core/bioawk/tests/main.nf.test     | 35 +++++++++++
 .../nf-core/bioawk/tests/main.nf.test.snap    | 37 ++++++++++++
 modules/nf-core/bioawk/tests/nextflow.config  |  6 ++
 8 files changed, 197 insertions(+)
 create mode 100644 modules/nf-core/bioawk/environment.yml
 create mode 100644 modules/nf-core/bioawk/main.nf
 create mode 100644 modules/nf-core/bioawk/meta.yml
 create mode 100644 modules/nf-core/bioawk/tests/main.nf.test
 create mode 100644 modules/nf-core/bioawk/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/bioawk/tests/nextflow.config

diff --git a/conf/modules.config b/conf/modules.config
index 777e7fb22..82e7e6da4 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -44,6 +44,17 @@ process {
         ]
     }
 
+    withName: 'BIOAWK' {
+        publishDir = [
+            path: { "${params.outdir}/bioawk" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+        ext.prefix =  { "${meta.id}_rrna.gtf" }
+        // Should we also have \$9 ~ /transcript_type "rRNA"/ as part of the filtering? See 's3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf' structure.
+        ext.args   = """-c gff '\$9 ~ /transcript_biotype "rRNA"/ || \$9 ~ /transcript_biotype "Mt_rRNA"/ || \$9 ~ /transcript_biotype "rRNA_pseudogene"/'"""
+    }
+
     withName: 'CTATSPLICING_STARTOCANCERINTRONS' {
         ext.args = {[
             bam ? "--vis" : "",
diff --git a/modules.json b/modules.json
index 972c32a9a..28bd2ce93 100644
--- a/modules.json
+++ b/modules.json
@@ -25,6 +25,11 @@
                         "git_sha": "98bd7be6d2191b0d5900ee698d91b52eeab90311",
                         "installed_by": ["modules"]
                     },
+                    "bioawk": {
+                        "branch": "master",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "installed_by": ["modules"]
+                    },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
diff --git a/modules/nf-core/bioawk/environment.yml b/modules/nf-core/bioawk/environment.yml
new file mode 100644
index 000000000..0be1e105f
--- /dev/null
+++ b/modules/nf-core/bioawk/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::bioawk=1.0
diff --git a/modules/nf-core/bioawk/main.nf b/modules/nf-core/bioawk/main.nf
new file mode 100644
index 000000000..a537dcf4a
--- /dev/null
+++ b/modules/nf-core/bioawk/main.nf
@@ -0,0 +1,38 @@
+process BIOAWK {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/bioawk:1.0--h5bf99c6_6':
+        'biocontainers/bioawk:1.0--h5bf99c6_6' }"
+
+    input:
+    tuple val(meta), path(input)
+
+    output:
+    tuple val(meta), path("*.gz"), emit: output
+    path "versions.yml"             , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args  = task.ext.args ?: '' // args is used for the main arguments of the tool
+    prefix = task.ext.prefix ?: "${meta.id}"
+    if ("${input}" == "${prefix}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    def VERSION = '1.0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    bioawk \\
+        $args \\
+        $input \\
+        > ${prefix}
+
+    gzip ${prefix}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        bioawk: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/bioawk/meta.yml b/modules/nf-core/bioawk/meta.yml
new file mode 100644
index 000000000..92b0a13c5
--- /dev/null
+++ b/modules/nf-core/bioawk/meta.yml
@@ -0,0 +1,58 @@
+name: "bioawk"
+description: Bioawk is an extension to Brian Kernighan's awk, adding the support of
+  several common biological data formats.
+keywords:
+  - bioawk
+  - fastq
+  - fasta
+  - sam
+  - file manipulation
+  - awk
+tools:
+  - "bioawk":
+      description: "BWK awk modified for biological data"
+      homepage: "https://github.com/lh3/bioawk"
+      documentation: "https://github.com/lh3/bioawk"
+      tool_dev_url: "https://github.com/lh3/bioawk"
+      licence: ["Free software license (https://github.com/lh3/bioawk/blob/master/README.awk#L1)"]
+      identifier: ""
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: Input sequence biological sequence file (optionally gzipped) to
+          be manipulated via program specified in `$args`.
+        pattern: "*.{bed,gff,sam,vcf,fastq,fasta,tab,bed.gz,gff.gz,sam.gz,vcf.gz,fastq.gz,fasta.gz,tab.gz}"
+        ontologies:
+          - edam: http://edamontology.org/format_1930 # FASTQ
+          - edam: http://edamontology.org/format_3475 # TSV
+output:
+  output:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gz":
+          type: file
+          description: |
+            Manipulated and gzipped version of input sequence file following program specified in `args`.
+            File name will be what is specified in `$prefix`. Do not include `.gz` suffix in `$prefix`! Output files` will be gzipped for you!
+          pattern: "*.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@jfy133"
+maintainers:
+  - "@jfy133"
diff --git a/modules/nf-core/bioawk/tests/main.nf.test b/modules/nf-core/bioawk/tests/main.nf.test
new file mode 100644
index 000000000..270ff1ef3
--- /dev/null
+++ b/modules/nf-core/bioawk/tests/main.nf.test
@@ -0,0 +1,35 @@
+
+nextflow_process {
+
+    name "Test Process BIOAWK"
+    script "../main.nf"
+    process "BIOAWK"
+    config "./nextflow.config"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "bioawk"
+
+    test("test-bioawk") {
+
+        when {
+            process {
+                """
+                input[0] = [
+				    [ id:'test', single_end:false ], // meta map
+				    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+				]
+
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+}
diff --git a/modules/nf-core/bioawk/tests/main.nf.test.snap b/modules/nf-core/bioawk/tests/main.nf.test.snap
new file mode 100644
index 000000000..fa9b59305
--- /dev/null
+++ b/modules/nf-core/bioawk/tests/main.nf.test.snap
@@ -0,0 +1,37 @@
+{
+    "test-bioawk": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "sample_1.fa.gz:md5,b558dd15d8940373a032a827d490e693"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,5fe88e58a71f10551df56518c35ba91a"
+                ],
+                "output": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "sample_1.fa.gz:md5,b558dd15d8940373a032a827d490e693"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,5fe88e58a71f10551df56518c35ba91a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-28T10:24:46.397249"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/bioawk/tests/nextflow.config b/modules/nf-core/bioawk/tests/nextflow.config
new file mode 100644
index 000000000..5ef017d97
--- /dev/null
+++ b/modules/nf-core/bioawk/tests/nextflow.config
@@ -0,0 +1,6 @@
+process {
+    withName: BIOAWK {
+        ext.args = "-c fastx \'{print \">\" \$name ORS length(\$seq)}\'"
+        ext.prefix = "sample_1.fa"
+    }
+}

From a23efe7229f04c9f0c5de6d299b9d02a4534db23 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 5 Aug 2025 15:07:44 +0000
Subject: [PATCH 738/887] chore: replace rrnatranscripts for bioawk

---
 subworkflows/local/build_references/main.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index f14f70135..c8cad74cb 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -15,7 +15,7 @@ include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatspl
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
-include { RRNATRANSCRIPTS                 } from '../../../modules/nf-core/rrnatranscripts/main'
+include { BIOAWK                          } from '../../../modules/nf-core/bioawk/main'
 include { AGAT_CONVERTGFF2BED             } from '../../../modules/nf-core/agat/convertgff2bed/main'
 include { ARRIBA_DOWNLOAD }                 from '../../../modules/nf-core/arriba/download/main'
 include { SAMTOOLS_FAIDX }                  from '../../../modules/nf-core/samtools/faidx/main'
@@ -85,10 +85,10 @@ workflow BUILD_REFERENCES {
             GATK4_CREATESEQUENCEDICTIONARY(ch_fasta)
             ch_versions = ch_versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
 
-            RRNATRANSCRIPTS(ch_gtf)
-            ch_versions = ch_versions.mix(RRNATRANSCRIPTS.out.versions)
+            BIOAWK(ch_gtf)
+            ch_versions = ch_versions.mix(BIOAWK.out.versions)
 
-            AGAT_CONVERTGFF2BED(RRNATRANSCRIPTS.out.rrna_gtf)
+            AGAT_CONVERTGFF2BED(BIOAWK.out.output)
             ch_versions = ch_versions.mix(AGAT_CONVERTGFF2BED.out.versions)
 
             GATK4_BEDTOINTERVALLIST(AGAT_CONVERTGFF2BED.out.bed, GATK4_CREATESEQUENCEDICTIONARY.out.dict )

From d45542728acd9a1c533038be96c50e4973c84fad Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 5 Aug 2025 15:32:23 +0000
Subject: [PATCH 739/887] chore: remove unused modules and scripts

---
 bin/get_rrna_transcripts.py                   |  81 ------------
 bin/gtf2bed                                   | 123 ------------------
 .../local/get_rrna_transcript/environment.yml |   6 -
 modules/local/get_rrna_transcript/main.nf     |  43 ------
 .../nf-core/rrnatranscripts/environment.yml   |   7 -
 modules/nf-core/rrnatranscripts/main.nf       |  34 -----
 modules/nf-core/rrnatranscripts/meta.yml      |  50 -------
 .../templates/get_rrna_transcripts.py         |  71 ----------
 .../rrnatranscripts/tests/main.nf.test        |  55 --------
 .../rrnatranscripts/tests/main.nf.test.snap   |  72 ----------
 10 files changed, 542 deletions(-)
 delete mode 100755 bin/get_rrna_transcripts.py
 delete mode 100755 bin/gtf2bed
 delete mode 100644 modules/local/get_rrna_transcript/environment.yml
 delete mode 100644 modules/local/get_rrna_transcript/main.nf
 delete mode 100644 modules/nf-core/rrnatranscripts/environment.yml
 delete mode 100644 modules/nf-core/rrnatranscripts/main.nf
 delete mode 100644 modules/nf-core/rrnatranscripts/meta.yml
 delete mode 100644 modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
 delete mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test
 delete mode 100644 modules/nf-core/rrnatranscripts/tests/main.nf.test.snap

diff --git a/bin/get_rrna_transcripts.py b/bin/get_rrna_transcripts.py
deleted file mode 100755
index 670d5f06c..000000000
--- a/bin/get_rrna_transcripts.py
+++ /dev/null
@@ -1,81 +0,0 @@
-#!/usr/bin/env python3
-
-import argparse
-import logging
-import sys
-from pathlib import Path
-
-
-def get_rrna_intervals(file_in, file_out):
-    """
-    Get lines containing ``#`` or ``gene_type rRNA`` or ```` or ``gene_type rRNA_pseudogene`` or ``gene_type MT_rRNA``
-    Create output file
-
-    Args:
-        file_in (pathlib.Path): The given GTF file.
-        file_out (pathlib.Path): Where the ribosomal RNA GTF file should
-            be created; always in GTF format.
-    """
-
-    patterns = {
-        "#",
-        'transcript_biotype "Mt_rRNA"',
-        'transcript_biotype "rRNA"',
-        'transcript_biotype "rRNA_pseudogene"',
-    }
-    line_starts = {"MT", "1", "2", "3", "4", "5", "6", "7", "8", "9"}
-    out_lines = []
-    with file_in.open() as f:
-        data = f.readlines()
-        for line in data:
-            for pattern in patterns:
-                if pattern in line:
-                    for line_start in line_starts:
-                        if line.startswith(line_start):
-                            out_lines.append(line)
-
-    with file_out.open(mode="w") as out_file:
-        out_file.writelines(out_lines)
-
-
-def parse_args(argv=None):
-    """Define and immediately parse command line arguments."""
-    parser = argparse.ArgumentParser(
-        description="Extract ribosomal RNA intervals from a gtf file.",
-        epilog="Example: python get_rrna_transcripts.py <filename.gtf> <intervals.gtf>",
-    )
-    parser.add_argument(
-        "file_in",
-        metavar="FILE_IN",
-        type=Path,
-        help="Input in GTF format.",
-    )
-    parser.add_argument(
-        "file_out",
-        metavar="FILE_OUT",
-        type=Path,
-        help="Transformed output intervals in GTF format.",
-    )
-    parser.add_argument(
-        "-l",
-        "--log-level",
-        help="The desired log level (default WARNING).",
-        choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"),
-        default="WARNING",
-    )
-    return parser.parse_args(argv)
-
-
-def main(argv=None):
-    """Coordinate argument parsing and program execution."""
-    args = parse_args(argv)
-    logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s")
-    if not args.file_in.is_file():
-        logger.error(f"The given input file {args.file_in} was not found!")
-        sys.exit(2)
-    args.file_out.parent.mkdir(parents=True, exist_ok=True)
-    get_rrna_intervals(args.file_in, args.file_out)
-
-
-if __name__ == "__main__":
-    sys.exit(main())
diff --git a/bin/gtf2bed b/bin/gtf2bed
deleted file mode 100755
index cfa91cf76..000000000
--- a/bin/gtf2bed
+++ /dev/null
@@ -1,123 +0,0 @@
-#!/usr/bin/env perl
-
-# Copyright (c) 2011 Erik Aronesty (erik@q32.com)
-#
-# Permission is hereby granted, free of charge, to any person obtaining a copy
-# of this software and associated documentation files (the "Software"), to deal
-# in the Software without restriction, including without limitation the rights
-# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
-# copies of the Software, and to permit persons to whom the Software is
-# furnished to do so, subject to the following conditions:
-#
-# The above copyright notice and this permission notice shall be included in
-# all copies or substantial portions of the Software.
-#
-# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
-# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
-# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
-# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
-# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
-# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
-# THE SOFTWARE.
-#
-# ALSO, IT WOULD BE NICE IF YOU LET ME KNOW YOU USED IT.
-
-use Getopt::Long;
-
-my $extended;
-GetOptions("x"=>\$extended);
-
-$in = shift @ARGV;
-
-my $in_cmd =($in =~ /\.gz$/ ? "gunzip -c $in|" : $in =~ /\.zip$/ ? "unzip -p $in|" : "$in") || die "Can't open $in: $!\n";
-open IN, $in_cmd;
-
-while (<IN>) {
-    $gff = 2 if /^##gff-version 2/;
-    $gff = 3 if /^##gff-version 3/;
-    next if /^#/ && $gff;
-
-    s/\s+$//;
-    # 0-chr 1-src 2-feat 3-beg 4-end 5-scor 6-dir 7-fram 8-attr
-    my @f = split /\t/;
-    if ($gff) {
-        # most ver 2's stick gene names in the id field
-        ($id) = $f[8]=~ /\bID="([^"]+)"/;
-        # most ver 3's stick unquoted names in the name field
-        ($id) = $f[8]=~ /\bName=([^";]+)/ if !$id && $gff == 3;
-    } else {
-        ($id) = $f[8]=~ /transcript_id "([^"]+)"/;
-    }
-
-    next unless $id && $f[0];
-
-    if ($f[2] eq 'exon') {
-        die "no position at exon on line $." if ! $f[3];
-        # gff3 puts :\d in exons sometimes
-        $id =~ s/:\d+$// if $gff == 3;
-        push @{$exons{$id}}, \@f;
-        # save lowest start
-        $trans{$id} = \@f if !$trans{$id};
-    } elsif ($f[2] eq 'start_codon') {
-        #optional, output codon start/stop as "thick" region in bed
-        $sc{$id}->[0] = $f[3];
-    } elsif ($f[2] eq 'stop_codon') {
-        $sc{$id}->[1] = $f[4];
-    } elsif ($f[2] eq 'miRNA' ) {
-        $trans{$id} = \@f if !$trans{$id};
-        push @{$exons{$id}}, \@f;
-    }
-}
-
-for $id (
-    # sort by chr then pos
-    sort {
-        $trans{$a}->[0] eq $trans{$b}->[0] ?
-        $trans{$a}->[3] <=> $trans{$b}->[3] :
-        $trans{$a}->[0] cmp $trans{$b}->[0]
-    } (keys(%trans)) ) {
-        my ($chr, undef, undef, undef, undef, undef, $dir, undef, $attr, undef, $cds, $cde) = @{$trans{$id}};
-        my ($cds, $cde);
-        ($cds, $cde) = @{$sc{$id}} if $sc{$id};
-
-        # sort by pos
-        my @ex = sort {
-            $a->[3] <=> $b->[3]
-        } @{$exons{$id}};
-
-        my $beg = $ex[0][3];
-        my $end = $ex[-1][4];
-
-        if ($dir eq '-') {
-        # swap
-        $tmp=$cds;
-        $cds=$cde;
-        $cde=$tmp;
-        $cds -= 2 if $cds;
-        $cde += 2 if $cde;
-    }
-
-    # not specified, just use exons
-    $cds = $beg if !$cds;
-    $cde = $end if !$cde;
-
-    # adjust start for bed
-    --$beg; --$cds;
-
-    my $exn = @ex;												# exon count
-    my $exst = join ",", map {$_->[3]-$beg-1} @ex;				# exon start
-    my $exsz = join ",", map {$_->[4]-$_->[3]+1} @ex;			# exon size
-
-        my $gene_id;
-        my $extend = "";
-        if ($extended) {
-            ($gene_id) = $attr =~ /gene_name "([^"]+)"/;
-            ($gene_id) = $attr =~ /gene_id "([^"]+)"/ unless $gene_id;
-            $extend="\t$gene_id";
-        }
-    # added an extra comma to make it look exactly like ucsc's beds
-    print "$chr\t$beg\t$end\t$id\t0\t$dir\t$cds\t$cde\t0\t$exn\t$exsz,\t$exst,$extend\n";
-}
-
-
-close IN;
diff --git a/modules/local/get_rrna_transcript/environment.yml b/modules/local/get_rrna_transcript/environment.yml
deleted file mode 100644
index 66b65c3a9..000000000
--- a/modules/local/get_rrna_transcript/environment.yml
+++ /dev/null
@@ -1,6 +0,0 @@
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::pirate=1.0.5
-  - bioconda::perl-bioperl=1.7.8
diff --git a/modules/local/get_rrna_transcript/main.nf b/modules/local/get_rrna_transcript/main.nf
deleted file mode 100644
index 901c17b42..000000000
--- a/modules/local/get_rrna_transcript/main.nf
+++ /dev/null
@@ -1,43 +0,0 @@
-process GET_RRNA_TRANSCRIPTS {
-    tag 'get_rrna_bed'
-    label 'process_low'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/pirate:1.0.5--hdfd78af_0' :
-        'biocontainers/pirate:1.0.5--hdfd78af_0' }"
-
-    input:
-    tuple val(meta), path(gtf)
-
-    output:
-    tuple val(meta), path('rrna.gtf')    , emit: rrnagtf
-    tuple val(meta), path('rrna.bed')    , emit: bed
-    path "versions.yml" , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    """
-    $baseDir/bin/get_rrna_transcripts.py ${gtf} rrna.gtf
-
-    $baseDir/bin/gtf2bed rrna.gtf > rrna.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        get_rrna_transcripts: v1.0
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch rrna.gtf
-    touch rrna.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        get_rrna_transcripts: v1.0
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/rrnatranscripts/environment.yml b/modules/nf-core/rrnatranscripts/environment.yml
deleted file mode 100644
index 6f09494b4..000000000
--- a/modules/nf-core/rrnatranscripts/environment.yml
+++ /dev/null
@@ -1,7 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - python=3.12.2
diff --git a/modules/nf-core/rrnatranscripts/main.nf b/modules/nf-core/rrnatranscripts/main.nf
deleted file mode 100644
index 67fa29b09..000000000
--- a/modules/nf-core/rrnatranscripts/main.nf
+++ /dev/null
@@ -1,34 +0,0 @@
-process RRNATRANSCRIPTS {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/python:3.12' :
-        'biocontainers/python:3.12' }"
-
-    input:
-    tuple val(meta), path(gtf)
-
-    output:
-    tuple val(meta), path('*_rrna_intervals.gtf')    , emit: rrna_gtf
-    path "versions.yml"                              , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    prefix = task.ext.prefix ?: "${meta.id}"
-    template 'get_rrna_transcripts.py'
-
-    stub:
-    prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}_rrna_intervals.gtf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        python: \$(python --version | sed -e "s/Python //g")
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/rrnatranscripts/meta.yml b/modules/nf-core/rrnatranscripts/meta.yml
deleted file mode 100644
index f793fbdd9..000000000
--- a/modules/nf-core/rrnatranscripts/meta.yml
+++ /dev/null
@@ -1,50 +0,0 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
-name: rrnatranscripts
-description: Ribosomal RNA extraction from a GTF file.
-keywords:
-  - ribosomal
-  - rna
-  - genomics
-tools:
-  - rrnatranscripts:
-      description: |
-        Extraction of ribosomal RNA
-      homepage: https://github.com/nf-core/rnafusion
-      licence: ["GPL-3.0-or-later"]
-      identifier: ""
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - gtf:
-        type: file
-        description: GTF file
-        pattern: "*.{gtf}"
-        ontologies:
-          - edam: "http://edamontology.org/format_2306" # GTF
-output:
-  rrna_gtf:
-    - - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*_rrna_intervals.gtf":
-          type: file
-          description: Output GTF file containing ribosomal RNA intervals
-          pattern: "*_rrna_intervals.gtf"
-          ontologies:
-            - edam: "http://edamontology.org/format_2306" # GTF
-  versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: "http://edamontology.org/format_3750" # YAML
-authors:
-  - "@rannick"
-maintainers:
-  - "@rannick"
diff --git a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py b/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
deleted file mode 100644
index b01c7d902..000000000
--- a/modules/nf-core/rrnatranscripts/templates/get_rrna_transcripts.py
+++ /dev/null
@@ -1,71 +0,0 @@
-#!/usr/bin/env python3
-
-"""
-Author:
-    Annick Renevey
-
-Copyright (c) 2025, Annick Renevey. All rights reserved.
-
-License: GPL-3 License
-"""
-
-import platform
-from pathlib import Path
-
-def format_yaml_like(data: dict, indent: int = 0) -> str:
-    """Formats a dictionary to a YAML-like string.
-
-    Args:
-        data (dict): The dictionary to format.
-        indent (int): The current indentation level.
-
-    Returns:
-        str: A string formatted as YAML.
-    """
-    yaml_str = ""
-    for key, value in data.items():
-        spaces = "  " * indent
-        if isinstance(value, dict):
-            yaml_str += f"{spaces}{key}:\\n{format_yaml_like(value, indent + 1)}"
-        else:
-            yaml_str += f"{spaces}{key}: {value}\\n"
-    return yaml_str
-
-def get_rrna_intervals(file_in: Path, file_out: Path):
-    """
-    Extracts rRNA lines from a GTF and writes them out.
-    """
-    patterns = {
-        "#",
-        'transcript_biotype "Mt_rRNA"',
-        'transcript_biotype "rRNA"',
-        'transcript_biotype "rRNA_pseudogene"',
-    }
-    line_starts = {"MT", "1", "2", "3", "4", "5", "6", "7", "8", "9"}
-    out_lines = []
-
-    with file_in.open() as f:
-        for line in f:
-            if any(p in line for p in patterns) and any(line.startswith(ls) for ls in line_starts):
-                out_lines.append(line)
-
-    file_out.parent.mkdir(parents=True, exist_ok=True)
-    with file_out.open("w") as out:
-        out.writelines(out_lines)
-
-# Main
-gtf_path  = Path("${gtf}")
-prefix    = "${prefix}"
-out_gtf   = Path(f"{prefix}_rrna_intervals.gtf")
-
-get_rrna_intervals(gtf_path, out_gtf)
-
-# Versions
-versions = {
-    "${task.process}": {
-        "python": platform.python_version()
-    }
-}
-
-with open("versions.yml", "w") as v:
-    v.write(format_yaml_like(versions))
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test b/modules/nf-core/rrnatranscripts/tests/main.nf.test
deleted file mode 100644
index 46ef3d048..000000000
--- a/modules/nf-core/rrnatranscripts/tests/main.nf.test
+++ /dev/null
@@ -1,55 +0,0 @@
-nextflow_process {
-
-    name "Test Process RRNATRANSCRIPTS"
-    script "../main.nf"
-    process "RRNATRANSCRIPTS"
-
-    tag "modules"
-    tag "modules_nfcore"
-    tag "rrnatranscripts"
-
-    test("homo_sapiens - gtf") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id: 'test', single_end: false ],  // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/riboseq_expression/Homo_sapiens.GRCh38.111_chr20.gtf', checkIfExists: true)
-                ]                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-    test("homo_sapiens - gtf - stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id: 'test', single_end: false ],  // meta map
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
-                ]                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-
-    }
-
-}
diff --git a/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap b/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
deleted file mode 100644
index 56b1f0955..000000000
--- a/modules/nf-core/rrnatranscripts/tests/main.nf.test.snap
+++ /dev/null
@@ -1,72 +0,0 @@
-{
-    "homo_sapiens - gtf": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_rrna_intervals.gtf:md5,db5d8f2c3cdd3135aeb1d6081e866caa"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,c1ef051fc08870f863ad611865f3f350"
-                ],
-                "rrna_gtf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_rrna_intervals.gtf:md5,db5d8f2c3cdd3135aeb1d6081e866caa"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,c1ef051fc08870f863ad611865f3f350"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-08-01T15:25:06.491928779"
-    },
-    "homo_sapiens - gtf - stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
-                ],
-                "rrna_gtf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test_rrna_intervals.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,fa2f785dbbe87a180f4254910957e01e"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-28T14:27:37.339133522"
-    }
-}
\ No newline at end of file

From 8750a112c81fe8a50638be1ec88c0148c58c32ec Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 6 Aug 2025 14:40:22 +0200
Subject: [PATCH 740/887] fix build test inputs

---
 tests/test_build.nf.test | 10 +++++-----
 1 file changed, 5 insertions(+), 5 deletions(-)

diff --git a/tests/test_build.nf.test b/tests/test_build.nf.test
index 6c00607f2..31e525185 100644
--- a/tests/test_build.nf.test
+++ b/tests/test_build.nf.test
@@ -18,11 +18,11 @@ nextflow_pipeline {
                 references_only            = true
                 input                      = "${projectDir}/tests/csv/fastq.csv"
                 genomes_base               = "${outputDir}/references"
-                fusioncatcher_ref          = null
-                arriba_ref_protein_domains = null
-                arriba_ref_known_fusions   = null
-                arriba_ref_blacklist       = null
-                arriba_ref_cytobands       = null
+                arriba_ref_protein_domains = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+                arriba_ref_known_fusions   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_blacklist       = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+                arriba_ref_cytobands       = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
+                fusioncatcher_ref          = "${projectDir}/assets" // This is a placeholder until a small reference can be created
                 no_cosmic                  = true
                 tools                      = "all"
                 fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'

From 8205a8eba61bf3461ab9d79305a263b4cefa1709 Mon Sep 17 00:00:00 2001
From: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 6 Aug 2025 16:52:21 +0200
Subject: [PATCH 741/887] test: update snapshot

---
 tests/test_build.nf.test.snap    |  30 ++-
 tests/test_stub.nf.test.snap     | 258 +++++++++++--------
 tests/test_stub_bam.nf.test.snap | 414 ++++++++++---------------------
 3 files changed, 297 insertions(+), 405 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 9e5959903..a8c988dc2 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -1,11 +1,17 @@
 {
     "Test build references": {
         "content": [
-            12,
+            13,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "4.0.1"
                 },
@@ -15,9 +21,6 @@
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
@@ -48,11 +51,12 @@
                 }
             },
             [
+                "agat",
+                "agat/minigenome.gtf.bed",
+                "bioawk",
+                "bioawk/minigenome.gtf_rrna.gtf.gz",
                 "gatk4",
                 "gatk4/minigenome.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
@@ -187,9 +191,9 @@
                 "references/starfusion/minigenome.fa_genome_lib_build_dir/trans.blast.dat.gz"
             ],
             [
+                "minigenome.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "minigenome.gtf_rrna.gtf.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
-                "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
                 "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -278,9 +282,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-17T18:35:50.278173296"
+        "timestamp": "2025-08-06T01:49:30.054631135"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 233b19ac6..47c65348c 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,8 +1,14 @@
 {
     "stub test starfusion": {
         "content": [
-            16,
+            17,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -19,9 +25,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -60,14 +63,15 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -209,15 +213,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-14T16:05:43.050813403"
+        "timestamp": "2025-08-06T02:30:33.359690799"
     },
     "stub test stringtie": {
         "content": [
-            16,
+            17,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -234,9 +244,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -273,14 +280,15 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -349,21 +357,27 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-16T09:24:22.233178"
+        "timestamp": "2025-08-06T02:32:17.345601567"
     },
     "stub test arriba": {
         "content": [
-            16,
+            17,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -380,9 +394,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -413,17 +424,18 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -490,15 +502,18 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-08T11:31:30.841349"
+        "timestamp": "2025-08-06T02:00:49.117095766"
     },
     "stub test fusioninspector": {
         "content": [
-            24,
+            25,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "AGAT_CONVERTSPGFF2TSV": {
                     "agat": "v1.4.1"
                 },
@@ -511,6 +526,9 @@
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -536,9 +554,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -581,12 +596,15 @@
             },
             [
                 "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
                 "arriba_visualisation",
                 "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -720,9 +738,6 @@
                 "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -876,15 +891,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-14T16:00:28.831228"
+        "timestamp": "2025-08-06T02:58:21.026238578"
     },
     "stub test salmon": {
         "content": [
-            11,
+            12,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -897,9 +918,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
@@ -920,14 +938,15 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -967,15 +986,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-16T09:27:23.823019"
+        "timestamp": "2025-08-06T02:47:44.805692669"
     },
     "stub test ctatsplicing": {
         "content": [
-            17,
+            18,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -998,9 +1023,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1036,6 +1058,10 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "ctatsplicing",
                 "ctatsplicing/test.cancer.introns",
                 "ctatsplicing/test.cancer.introns.prelim",
@@ -1053,9 +1079,6 @@
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1198,21 +1221,27 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-14T16:03:10.060575954"
+        "timestamp": "2025-08-06T02:13:41.676105751"
     },
     "stub test fusionreport": {
         "content": [
-            22,
+            23,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1242,9 +1271,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1283,9 +1309,13 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1303,9 +1333,6 @@
                 "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1461,15 +1488,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-22T13:42:49.769985"
+        "timestamp": "2025-08-06T02:45:36.593183242"
     },
     "stub test fastp": {
         "content": [
-            10,
+            11,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
@@ -1488,9 +1521,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1502,6 +1532,10 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -1516,9 +1550,6 @@
                 "fastqc_for_fastp/test_trimmed.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1535,15 +1566,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
+            "nf-test": "0.9.0",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-06-16T09:26:06.949386"
+        "timestamp": "2025-08-06T02:46:43.738246283"
     },
     "stub test fusioncatcher": {
         "content": [
-            10,
+            11,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1563,9 +1600,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1577,6 +1611,10 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1586,9 +1624,6 @@
                 "fusioncatcher/test.summary.txt",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1608,15 +1643,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-22T13:36:15.064231"
+        "timestamp": "2025-08-06T02:14:58.686768786"
     },
     "stub test fusioncatcher trim": {
         "content": [
-            11,
+            12,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTP_FOR_FUSIONCATCHER": {
                     "fastp": "0.24.0"
                 },
@@ -1639,9 +1680,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1653,6 +1691,10 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastp_for_fusioncatcher",
                 "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.html",
                 "fastp_for_fusioncatcher/test_trimmed_for_fusioncatcher.fastp.json",
@@ -1668,9 +1710,6 @@
                 "fusioncatcher/test.summary.txt",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1690,15 +1729,18 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-22T13:37:38.01216"
+        "timestamp": "2025-08-06T02:16:14.659256975"
     },
     "stub test all": {
         "content": [
-            36,
+            37,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "AGAT_CONVERTSPGFF2TSV": {
                     "agat": "v1.4.1"
                 },
@@ -1711,6 +1753,9 @@
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -1755,9 +1800,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
@@ -1818,12 +1860,15 @@
             },
             [
                 "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
                 "arriba_visualisation",
                 "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "ctatsplicing",
                 "ctatsplicing/test.cancer.introns",
                 "ctatsplicing/test.cancer.introns.prelim",
@@ -1982,9 +2027,6 @@
                 "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -2180,9 +2222,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-22T13:28:04.670405"
+        "timestamp": "2025-08-06T01:59:19.989117625"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 1755325a9..48cb4aafb 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -1,8 +1,14 @@
 {
     "stub test starfusion": {
         "content": [
-            14,
+            15,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -19,9 +25,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -52,14 +55,15 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -182,15 +186,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-14T15:48:07.635249587"
+        "timestamp": "2025-08-06T03:21:00.500662919"
     },
     "stub test stringtie": {
         "content": [
-            15,
+            16,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -207,9 +217,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -238,14 +245,15 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -298,21 +306,27 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-02T17:05:41.044302"
+        "timestamp": "2025-08-06T03:22:15.713895494"
     },
     "stub test arriba": {
         "content": [
-            14,
+            15,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -329,9 +343,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -354,17 +365,18 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -412,15 +424,18 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-08T12:57:24.512309"
+        "timestamp": "2025-08-06T03:11:10.337479886"
     },
     "stub test fusioninspector": {
         "content": [
-            23,
+            24,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "AGAT_CONVERTSPGFF2TSV": {
                     "agat": "v1.4.1"
                 },
@@ -433,6 +448,9 @@
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -458,9 +476,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -495,12 +510,15 @@
             },
             [
                 "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
                 "arriba_visualisation",
                 "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -634,9 +652,6 @@
                 "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -774,15 +789,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-14T16:53:36.788101"
+        "timestamp": "2025-08-06T03:41:21.307526426"
     },
     "stub test salmon": {
         "content": [
-            14,
+            15,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -799,9 +820,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
@@ -828,14 +846,15 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -884,228 +903,46 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-03T10:34:40.653967"
+        "timestamp": "2025-08-06T03:33:59.679716364"
     },
     "stub test ctatsplicing": {
         "content": [
-            16,
+            0,
             {
-                "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "CTATSPLICING_STARTOCANCERINTRONS": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION_BUILD": {
-                    "gunzip": 1.12,
-                    "hmmer": 3.4,
-                    "STAR-Fusion": "1.15.1"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "ctatsplicing",
-                "ctatsplicing/test.cancer.introns",
-                "ctatsplicing/test.cancer.introns.prelim",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/test.chckpts",
-                "ctatsplicing/test.ctat-splicing.igv.html",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/test.igv.tracks",
-                "ctatsplicing/test.introns",
-                "ctatsplicing/test.introns.for_IGV.bed",
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
-                "references",
-                "references/gencode",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/star",
-                "references/star/Genome",
-                "references/star/Log.out",
-                "references/star/SA",
-                "references/star/SAindex",
-                "references/star/chrLength.txt",
-                "references/star/chrName.txt",
-                "references/star/chrNameLength.txt",
-                "references/star/chrStart.txt",
-                "references/star/exonGeTrInfo.tab",
-                "references/star/exonInfo.tab",
-                "references/star/geneInfo.tab",
-                "references/star/genomeParameters.txt",
-                "references/star/sjdbInfo.txt",
-                "references/star/sjdbList.fromGTF.out.tab",
-                "references/star/sjdbList.out.tab",
-                "references/star/transcriptInfo.tab",
-                "references/starfusion",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/blast_pairs.idx",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/cancer_splicing_lib",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/pfam_domains.dbm",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/refGene.bed",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/refGene.sort.bed.gz",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cds",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.pep",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.not",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/starfusion/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa_genome_lib_build_dir/trans.blast.dat.gz",
-                "star",
-                "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai"
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-14T15:46:23.95688627"
+        "timestamp": "2025-08-06T03:11:35.363568093"
     },
     "stub test fusionreport": {
         "content": [
-            20,
+            21,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
                 "ARRIBA_DOWNLOAD": {
                     "arriba_download": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1135,9 +972,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1168,9 +1002,13 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1188,9 +1026,6 @@
                 "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1327,15 +1162,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-22T14:08:17.97268"
+        "timestamp": "2025-08-06T03:31:36.419602575"
     },
     "stub test fastp": {
         "content": [
-            13,
+            14,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
@@ -1358,9 +1199,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1378,6 +1216,10 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastp",
                 "fastp/test.fastp.html",
                 "fastp/test.fastp.json",
@@ -1392,9 +1234,6 @@
                 "fastqc_for_fastp/test_trimmed.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1420,15 +1259,21 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "24.10.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-05-07T13:29:36.326072757"
+        "timestamp": "2025-08-06T03:32:51.98792128"
     },
     "stub test fusioncatcher": {
         "content": [
-            13,
+            14,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
@@ -1452,9 +1297,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GTF_TO_REFFLAT": {
                     "gtfToGenePred": 377
                 },
@@ -1472,6 +1314,10 @@
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
@@ -1481,9 +1327,6 @@
                 "fusioncatcher/test.summary.txt",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1512,15 +1355,18 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-22T14:03:08.199969"
+        "timestamp": "2025-08-06T03:13:18.315049907"
     },
     "stub test all": {
         "content": [
-            35,
+            36,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
                 "AGAT_CONVERTSPGFF2TSV": {
                     "agat": "v1.4.1"
                 },
@@ -1533,6 +1379,9 @@
                 "ARRIBA_VISUALISATION": {
                     "arriba": "2.5.0"
                 },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -1577,9 +1426,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
@@ -1632,12 +1478,15 @@
             },
             [
                 "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
                 "arriba_visualisation",
                 "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "ctatsplicing",
                 "ctatsplicing/test.cancer.introns",
                 "ctatsplicing/test.cancer.introns.prelim",
@@ -1796,9 +1645,6 @@
                 "fusionreport/test/test_fusionreport_index.html",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1978,9 +1824,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.0",
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-07-22T13:58:16.809449"
+        "timestamp": "2025-08-06T03:09:39.3024867"
     }
-}
+}
\ No newline at end of file

From 773bc65a163dfb8019947b4ca75307515cfc6be8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 7 Aug 2025 12:49:33 +0200
Subject: [PATCH 742/887] update snap

---
 tests/test_stub.nf.test.snap | 12 +++++++-----
 1 file changed, 7 insertions(+), 5 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index d7934562c..0be223c1f 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -568,7 +568,8 @@
                     "gawk": "5.1.0"
                 },
                 "VCF_COLLECT": {
-                    "python": "3.11.0"
+                    "python": "3.11.0",
+                    "vcf_collect": 0.1
                 },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
@@ -871,7 +872,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T15:02:29.884427377"
+        "timestamp": "2025-08-07T11:57:10.372482"
     },
     "stub test salmon": {
         "content": [
@@ -1776,7 +1777,8 @@
                     "stringtie": "2.2.3"
                 },
                 "VCF_COLLECT": {
-                    "python": "3.11.0"
+                    "python": "3.11.0",
+                    "vcf_collect": 0.1
                 },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
@@ -2141,6 +2143,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T14:53:33.739317046"
+        "timestamp": "2025-08-07T10:38:44.804072"
     }
-}
+}
\ No newline at end of file

From 2dbc1e62e838860085c2915eff2cbfdee5c3ba9e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 7 Aug 2025 12:56:51 +0200
Subject: [PATCH 743/887] remove usage of cosmic in tests

---
 .github/workflows/awsfulltest.yml |  3 +--
 .github/workflows/awstest.yml     |  3 +--
 conf/test_cosmic.config           | 31 -------------------------------
 3 files changed, 2 insertions(+), 35 deletions(-)
 delete mode 100644 conf/test_cosmic.config

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 2567143f0..e40c48160 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -35,9 +35,8 @@ jobs:
               "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}",
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ steps.revision.outputs.revision }}"
               "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}/references",
-              "cosmic_username": "${{ secrets.cosmic_username }}",
-              "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
               "all": true,
+              "no_cosmic": true
             }
           profiles: test_full
 
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 3010abb67..07fae34c7 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -23,10 +23,9 @@ jobs:
             {
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}",
               "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}/references",
-              "cosmic_username": "${{ secrets.cosmic_username }}",
-              "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
               "all": true,
               "stub": true
+              "no_cosmic": true"
             }
           profiles: test
 
diff --git a/conf/test_cosmic.config b/conf/test_cosmic.config
deleted file mode 100644
index 9cc1bcda9..000000000
--- a/conf/test_cosmic.config
+++ /dev/null
@@ -1,31 +0,0 @@
-/*
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Nextflow config file for running minimal tests
-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-    Defines input files and everything required to run a fast and simple pipeline test.
-
-    Use as follows:
-        nextflow run nf-core/rnafusion -profile test_cosmic,<docker/singularity> --outdir <OUTDIR> -stub
-
-----------------------------------------------------------------------------------------
-*/
-
-params {
-    config_profile_name        = 'Test cosmic profile'
-    config_profile_description = 'Minimal test cosmic dataset to check pipeline function'
-
-    // Input data
-    input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-    all                    = true
-    cosmic_username        = secrets.COSMIC_USERNAME
-    cosmic_passwd          = secrets.COSMIC_PASSWD
-}
-
-// Limit and standardize resources for github actions and reproducibility
-process {
-    resourceLimits = [
-        cpus: 4,
-        memory: '15.GB',
-        time: '1.h'
-    ]
-}

From 292e6822ffa8cce8641fcce4f952dd15c751aadc Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 7 Aug 2025 13:01:18 +0200
Subject: [PATCH 744/887] apply some fixes to the configs

---
 conf/test_build.config  | 1 +
 conf/test_cosmic.config | 2 +-
 conf/test_full.config   | 2 +-
 nextflow.config         | 5 ++---
 4 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index ca76db564..539fabc77 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -36,6 +36,7 @@ params {
     // Input data
     references_only            = true
     input                      = "${projectDir}/tests/csv/fastq.csv"
+    genomes_base               = "${params.outdir}/references"
     no_cosmic                  = true
     tools                      = "arriba,starfusion,fusionreport,salmon,fusioninspector"
     fasta                      = 'https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa'
diff --git a/conf/test_cosmic.config b/conf/test_cosmic.config
index 9cc1bcda9..395bebb0a 100644
--- a/conf/test_cosmic.config
+++ b/conf/test_cosmic.config
@@ -16,7 +16,7 @@ params {
 
     // Input data
     input                  = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
-    all                    = true
+    tools                  = "all"
     cosmic_username        = secrets.COSMIC_USERNAME
     cosmic_passwd          = secrets.COSMIC_PASSWD
 }
diff --git a/conf/test_full.config b/conf/test_full.config
index cd1992fea..e1ff7f4b1 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -16,4 +16,4 @@ params {
     input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv'
     tools = "all"
 
-    }
+}
diff --git a/nextflow.config b/nextflow.config
index 7ceae0938..48a8badd1 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -216,7 +216,7 @@ profiles {
     test_cosmic {
         includeConfig 'conf/test_cosmic.config'
     }
-        test_full {
+    test_full {
         includeConfig 'conf/test_full.config'
     }
 
@@ -237,8 +237,6 @@ profiles {
         apptainer.runOptions    = '--nv'
         singularity.runOptions  = '--nv'
     }
-    test      { includeConfig 'conf/test.config'      }
-    test_full { includeConfig 'conf/test_full.config' }
 }
 
 // Load nf-core custom profiles from different institutions
@@ -368,6 +366,7 @@ validation {
     summary {
         beforeText = validation.help.beforeText
         afterText = validation.help.afterText
+        hideParams = ["cosmic_username", "cosmic_passwd"]
     }
 }
 

From b8eec288ea075ce3733ae76e86880b02d6adf281 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 7 Aug 2025 13:07:58 +0200
Subject: [PATCH 745/887] fix awstest params

---
 .github/workflows/awsfulltest.yml | 4 ++--
 .github/workflows/awstest.yml     | 4 ++--
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 2567143f0..13deb34bc 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -34,10 +34,10 @@ jobs:
             {
               "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}",
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ steps.revision.outputs.revision }}"
-              "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}/references",
+              "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/references",
               "cosmic_username": "${{ secrets.cosmic_username }}",
               "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
-              "all": true,
+              "tools": "all",
             }
           profiles: test_full
 
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 3010abb67..8eed04bcc 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -22,10 +22,10 @@ jobs:
           parameters: |
             {
               "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}",
-              "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/results-${{ github.sha }}/references",
+              "genomes_base": "s3://${{ secrets.AWS_S3_BUCKET }}/rnafusion/references",
               "cosmic_username": "${{ secrets.cosmic_username }}",
               "cosmic_passwd": "${{ secrets.cosmic_passwd }}",
-              "all": true,
+              "tools": "all",
               "stub": true
             }
           profiles: test

From 3c633f1240aa9b65516c9c53031af27aa723974c Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 7 Aug 2025 13:57:23 +0200
Subject: [PATCH 746/887] remove typo

---
 modules/local/starfusion/detect/tests/main.nf.test | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
index 63d91b12b..2dec2d2cf 100644
--- a/modules/local/starfusion/detect/tests/main.nf.test
+++ b/modules/local/starfusion/detect/tests/main.nf.test
@@ -20,7 +20,7 @@ nextflow_process {
 
                 input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                 input[3] = "homo_sapiens"
-                input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"git
+                input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
                 input[5] = [
                     "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
                     "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",

From 9ea86a05bf9e1a9177b4f7f4d8f447dc9b590c73 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 7 Aug 2025 15:15:24 +0200
Subject: [PATCH 747/887] update snap

---
 tests/test_stub_bam.nf.test.snap | 12 +++++++-----
 1 file changed, 7 insertions(+), 5 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 3cbcf2ad4..041d94837 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -482,7 +482,8 @@
                     "gawk": "5.1.0"
                 },
                 "VCF_COLLECT": {
-                    "python": "3.11.0"
+                    "python": "3.11.0",
+                    "vcf_collect": 0.1
                 },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
@@ -769,7 +770,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T15:17:08.528288381"
+        "timestamp": "2025-08-07T15:10:07.731105"
     },
     "stub test salmon": {
         "content": [
@@ -1595,7 +1596,8 @@
                     "stringtie": "2.2.3"
                 },
                 "VCF_COLLECT": {
-                    "python": "3.11.0"
+                    "python": "3.11.0",
+                    "vcf_collect": 0.1
                 },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
@@ -1944,6 +1946,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T15:04:39.683600348"
+        "timestamp": "2025-08-07T13:56:14.46501"
     }
-}
+}
\ No newline at end of file

From 6144f66693133c1ecb390b1d6439a354222c3643 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 7 Aug 2025 14:24:09 +0000
Subject: [PATCH 748/887] remove arriba_download

---
 subworkflows/local/build_references/main.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index fc917e57a..eecec62ef 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -16,7 +16,6 @@ include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatspl
 */
 include { BIOAWK                          } from '../../../modules/nf-core/bioawk/main'
 include { AGAT_CONVERTGFF2BED             } from '../../../modules/nf-core/agat/convertgff2bed/main'
-include { ARRIBA_DOWNLOAD }                 from '../../../modules/nf-core/arriba/download/main'
 include { SAMTOOLS_FAIDX }                  from '../../../modules/nf-core/samtools/faidx/main'
 include { STAR_GENOMEGENERATE }             from '../../../modules/nf-core/star/genomegenerate/main'
 include { GATK4_CREATESEQUENCEDICTIONARY }  from '../../../modules/nf-core/gatk4/createsequencedictionary/main'

From 71fc45fc67726192487d4d2b1f7c4ef6ecaadb08 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 8 Aug 2025 09:59:37 +0200
Subject: [PATCH 749/887] fix issues and add characters to fusioninspectore
 stub run so the downwards analysis is still triggered

---
 modules/local/fusioninspector/main.nf          | 2 +-
 nextflow.config                                | 3 ---
 subworkflows/local/fusioninspector_workflow.nf | 7 ++++---
 3 files changed, 5 insertions(+), 7 deletions(-)

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
index 95ab06735..4d0507011 100644
--- a/modules/local/fusioninspector/main.nf
+++ b/modules/local/fusioninspector/main.nf
@@ -60,7 +60,7 @@ process FUSIONINSPECTOR {
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch FusionInspector.log
-    touch ${prefix}.FusionInspector.fusions.abridged.tsv
+    echo "this is a stub run" > ${prefix}.FusionInspector.fusions.abridged.tsv
     touch ${prefix}.FusionInspector.fusions.tsv
     touch ${prefix}.fusion_inspector_web.html
     mkdir -p chckpts_dir
diff --git a/nextflow.config b/nextflow.config
index 48a8badd1..a39625e12 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -213,9 +213,6 @@ profiles {
     test_build {
         includeConfig 'conf/test_build.config'
     }
-    test_cosmic {
-        includeConfig 'conf/test_cosmic.config'
-    }
     test_full {
         includeConfig 'conf/test_full.config'
     }
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index c4e5d2c3d..c9c2b600c 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -42,14 +42,15 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         FUSIONINSPECTOR( ch_reads_fusion, ch_starfusion_ref)
         ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions)
 
-        def tsv_nonempty = FUSIONINSPECTOR.out.tsv.filter { meta, file -> file.exists() && file.size() > 0 }
-        def tsv_abridged_nonempty = FUSIONINSPECTOR.out.abridged_tsv.filter { meta, file -> file.exists() && file.size() > 0 }
+        def tsv_nonempty = FUSIONINSPECTOR.out.tsv.filter { _meta, file -> file.exists() && file.size() > 0 }
+        def tsv_abridged_nonempty = FUSIONINSPECTOR.out.abridged_tsv.filter { _meta, file -> file.exists() && file.size() > 0 }
 
-        def gtf_nonempty = FUSIONINSPECTOR.out.out_gtf.filter { meta, file -> file.exists() && file.size() > 0 }
+        def gtf_nonempty = FUSIONINSPECTOR.out.out_gtf.filter { _meta, file -> file.exists() && file.size() > 0 }
 
         if (
             !skip_vcf &&
             tsv_nonempty &&
+            tsv_abridged_nonempty &&
             gtf_nonempty
         ) {
             AGAT_CONVERTSPGFF2TSV(gtf_nonempty)

From 4f3f26786eb3cd71369e6be78dfd3efaa18099e9 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 8 Aug 2025 12:32:25 +0200
Subject: [PATCH 750/887] apply  first updates

---
 README.md                | 69 ++++++++++++++++------------------------
 assets/schema_input.json |  1 +
 docs/usage.md            | 52 +++++++++++++++---------------
 workflows/rnafusion.nf   |  1 -
 4 files changed, 55 insertions(+), 68 deletions(-)

diff --git a/README.md b/README.md
index 8c8b0f845..1645c0ca3 100644
--- a/README.md
+++ b/README.md
@@ -20,7 +20,7 @@
 
 ## Introduction
 
-**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.
+**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.
 
 On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).
 
@@ -40,65 +40,52 @@ aws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <p
 
 The path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.
 
-:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**
+⚠️ **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**
 
-The pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only genocode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.
+The pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.
 
 1. Download gencode fasta and gtf files
-2. Create [STAR](https://github.com/alexdobin/STAR) index
-3. Download [Arriba](https://github.com/suhrig/arriba) references
-4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references
-5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references
-6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs
+2. Download the HGNC nomenclature file
+3. Create files needed for QC (Sequence Dictionary and RRNA intervals)
+4. Convert the gtf file to a refflat file
+5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index
+6. Create [STAR](https://github.com/alexdobin/STAR) index
+7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references
+8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs
+
+> [!WARNING]
+> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source.
 
 #### Main workflow
 
 1. Input samplesheet check
-2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))
-3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
-4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)
-5. Arriba subworkflow
-   - [STAR](https://github.com/alexdobin/STAR) alignment
-   - [Arriba](https://github.com/suhrig/arriba) fusion detection
-6. STAR-fusion subworkflow
-   - [STAR](https://github.com/alexdobin/STAR) alignment
-   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection
-7. Fusioncatcher subworkflow
-   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection
-8. StringTie subworkflow
-   - [StringTie](https://ccb.jhu.edu/software/stringtie/)
-9. Fusion-report
-   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)
-10. Post-processing and analysis of data
+2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
+3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)
+4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)
+5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)
+6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
+7. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
+8. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)
+9. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)
+10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)
+11. Post-processing and analysis of data
     - [FusionInspector](https://github.com/FusionInspector/FusionInspector)
     - [Arriba](https://github.com/suhrig/arriba) visualisation
-    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
-11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
-12. Compress bam files to cram with [samtools view](http://www.htslib.org/)
+    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
+12. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+13. Compress bam files to cram with [samtools view](http://www.htslib.org/)
 
 ## Usage
 
 > [!NOTE]
 > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
 
-As the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):
-
-First, build the references:
-
-```bash
-nextflow run nf-core/rnafusion \
-   -profile test,<docker/singularity/.../institute> \
-   --outdir <OUTDIR>\
-   --references_only \
-   -stub
-```
-
-Then perform the analysis:
+As the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.
 
 ```bash
 nextflow run nf-core/rnafusion \
    -profile test,<docker/singularity/.../institute> \
-   --outdir <OUTDIR>\
+   --outdir <OUTDIR> \
    -stub
 ```
 
diff --git a/assets/schema_input.json b/assets/schema_input.json
index bbd076f56..c0ff9aea6 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -87,6 +87,7 @@
                 "meta": "seq_center"
             }
         },
+        "uniqueEntries": ["sample"],
         "dependentRequired": {
             "bai": ["bam"],
             "crai": ["cram"]
diff --git a/docs/usage.md b/docs/usage.md
index 5c3905b99..16228338d 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -10,11 +10,10 @@ The pipeline is divided into two parts:
 
 1. Download and build references
 
-- specified with `--references_only` parameter
-- required only once before running the pipeline
-- **Important**: has to be run with each new release
+- Download the references from the S3 bucket (recommended) or build them with the pipeline
+- **Important**: has to be done with each new release
 
-2. Detecting fusions
+2. Running the analysis
 
 - Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, `StringTie` and `CTAT-SPLICING`
 - QC: `Fastqc`, `MultiQC`, and `Picard CollectInsertSize`, `Picard CollectWgsMetrics`, `Picard Markduplicates`
@@ -32,7 +31,8 @@ aws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <p
 
 The path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.
 
-:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**
+> [!WARNING]
+> Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.
 
 The fusion report references available on the S3 bucket do not contain information from cosmic due to licensing issues. If you want to use the cosmic database, you will need to build the fusion report references yourself by either deleting the `fusion_report_db` folder in the references directory or by specifying a different location for the fusion report directory with `--fusionreport_ref <PATH/TO/REFERENCES>`. The cosmic username and password should also be given in this case using `--cosmic_username <EMAIL>` and `--cosmic_passwd <PASSWORD>`.
 
@@ -124,8 +124,11 @@ The references are only built based on ensembl version 102. It is not possible c
 
 ### Samplesheet input
 
-You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. The pipeline will detect whether a sample is single- or paired-end from the samplesheet - the `fastq_2` column is empty for single-end. The samplesheet has to be a comma-separated (.csv), tab-separated (.tsv), yaml (.yaml or .yml) or json (.json) file but can have as many columns as you desire. There is a strict requirement for the `sample` and `strandedness` columns. One or more of these columns should be provided too: `fastq_1`, `bam`, `cram`, `junctions` and `splice_junctions`
-A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice.
+You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. The pipeline will detect whether a sample is single- or paired-end from the samplesheet - the `fastq_2` column is empty for single-end. 
+
+The samplesheet has to be a comma-separated (.csv), tab-separated (.tsv), yaml (.yaml or .yml) or json (.json) file but can have as many entries as you desire. There is a strict requirement for the `sample` and `strandedness` fields. One or more of these fields should be provided too: `fastq_1`, `bam`, `cram`, `junctions` and `splice_junctions`
+
+A final samplesheet file consisting of both single- and paired-end data may look something like the one below:
 
 ```csv title="samplesheet.csv"
 sample,fastq_1,fastq_2,strandedness
@@ -135,29 +138,26 @@ CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz,f
 TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz,,forward
 TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,,forward
 TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,forward
-TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,forward
 ```
 
-As you can see above for multiple runs of the same sample, the `sample` name has to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Note that multiple rows per sample are not supported for samples that contain `bam`, `cram`, `junctions` and/or `splice_junctions` files.
-
 | Column             | Description                                                                                                                                                                                                                                                                                                                                                                                                                                   | Required           |
 | ------------------ | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
-| `sample`           | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`).                                                                                                                                                                                                                                                        | :white_check_mark: |
-| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                                                                                                             | :white_check_mark: |
-| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.                                                                                      | :grey_question:    |
-| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                      | :grey_question:    |
-| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.   | :grey_question:    |
-| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                                                                                                                 | :x:                |
-| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty. | :grey_question:    |
-| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                                                                                                               | :x:                |
-| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.      | :grey_question:    |
-| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                | :grey_question:    |
-| `seq_platform`     | The sequencing platform used to create to sequence the data in the FASTQ files. This value will take precedence over the value provided with `--seq_platform`.                                                                                                                                                                                                                                                                                | :x:                |
-| `seq_center`       | The sequencing center in which the data in the FASTQ files was sequenced. This value will take precedence over the value provided with `--seq_center`.                                                                                                                                                                                                                                                                                        | :x:                |
-
-:white_check_mark: = Required
-:x: = Not required
-:grey_question: = One of these columns should be provided
+| `sample`           | Custom sample name. This value needs to be unique across all entries in the samplesheet and cannot contain spaces                                                                                                                                                                                                                                                     | ✅ |
+| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                                                                                                             | ✅ |
+| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.                                                                                      | ❓    |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                      | ❓    |
+| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.   | ❓    |
+| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                                                                                                                 | ❌                |
+| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty. | ❓    |
+| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                                                                                                               | ❌                |
+| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.      | ❓    |
+| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                | ❓    |
+| `seq_platform`     | The sequencing platform used to create to sequence the data in the FASTQ files. This value will take precedence over the value provided with `--seq_platform`.                                                                                                                                                                                                                                                                                | ❌                |
+| `seq_center`       | The sequencing center in which the data in the FASTQ files was sequenced. This value will take precedence over the value provided with `--seq_center`.                                                                                                                                                                                                                                                                                        | ❌                |
+
+✅ = Required
+❌ = Not required
+❓ = One of these columns should be provided
 
 ### Preparing BAM/CRAM/junctions/splice_junctions
 
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2bb866c21..e07ddf674 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -5,7 +5,6 @@
 */
 
 include { BUILD_REFERENCES              }   from '../subworkflows/local/build_references'
-include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
 include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow/main'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
 include { STARFUSION                    }   from '../modules/local/starfusion/detect/main'

From 449eab0095de5a70fef6657ac6c9a52b0d0f6b2f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 11:25:14 +0000
Subject: [PATCH 751/887] tests: update test build

---
 conf/test_build.config        |  5 ++++-
 tests/test_build.nf.test.snap | 18 ++++++------------
 2 files changed, 10 insertions(+), 13 deletions(-)

diff --git a/conf/test_build.config b/conf/test_build.config
index 539fabc77..8fbb75ba5 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -18,7 +18,6 @@ This test is designed to test the `build_references` subworkflow with the follow
 - GTF_TO_REFFLAT
 - GFFREAD
 - STAR_GENOMEGENERATE
-- ARRIBA_DOWNLOAD
 - STARFUSION_BUILD
 - FUSIONREPORT_DOWNLOAD
 
@@ -52,6 +51,10 @@ params {
     dfam_h3m                   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m"
     dfam_h3p                   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
     pfam_file                  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
+    arriba_ref_protein_domains = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
+    arriba_ref_known_fusions   = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_blacklist       = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
+    arriba_ref_cytobands       = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"
 
 }
 
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 79b31374a..ee462d5d6 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -6,14 +6,12 @@
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
+                "CTATSPLICING_PREPGENOMELIB": {
+                    "ctat-splicing": "0.0.2"
+                },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "4.0.1"
                 },
@@ -196,10 +194,6 @@
                 "minigenome.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.gtf_rrna.gtf.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
-                "cytobands_hg38_GRCh38_v2.5.0.tsv:md5,7bd504feefb33fcfc9be0517439a423c",
-                "protein_domains_hg38_GRCh38_v2.5.0.gff3:md5,43c387a784ebeed71b4147076cebf978",
-                "rrna.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "rrna.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
@@ -288,9 +282,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T01:49:30.054631135"
+        "timestamp": "2025-08-08T11:11:40.714960564"
     }
 }
\ No newline at end of file

From 79bb0dba2d15c926bd73b188b086cb1635e7536f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 11:29:06 +0000
Subject: [PATCH 752/887] test: update test stub snapshot

---
 tests/test_stub.nf.test.snap | 84 +++++++++++++++++-------------------
 1 file changed, 39 insertions(+), 45 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 9f38b0b18..a8212ba9d 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -215,10 +215,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:30:33.359690799"
+        "timestamp": "2025-08-08T11:20:09.057111889"
     },
     "stub test stringtie": {
         "content": [
@@ -361,14 +361,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:32:17.345601567"
+        "timestamp": "2025-08-08T11:20:46.857913005"
     },
     "stub test arriba": {
         "content": [
-            17,
+            16,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -376,9 +376,6 @@
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
@@ -503,14 +500,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:00:49.117095766"
+        "timestamp": "2025-08-08T11:16:32.896368369"
     },
     "stub test fusioninspector": {
         "content": [
-            25,
+            24,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -887,10 +884,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:58:21.026238578"
+        "timestamp": "2025-08-08T11:23:44.5776928"
     },
     "stub test salmon": {
         "content": [
@@ -982,10 +979,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:47:44.805692669"
+        "timestamp": "2025-08-08T11:22:26.942424142"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -1219,14 +1216,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:13:41.676105751"
+        "timestamp": "2025-08-08T11:17:54.284398814"
     },
     "stub test fusionreport": {
         "content": [
-            23,
+            21,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1234,9 +1231,6 @@
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
@@ -1476,10 +1470,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:45:36.593183242"
+        "timestamp": "2025-08-08T11:27:41.801614257"
     },
     "stub test fastp": {
         "content": [
@@ -1556,14 +1550,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:46:43.738246283"
+        "timestamp": "2025-08-08T11:21:51.143010384"
     },
     "stub test fusioncatcher": {
         "content": [
-            11,
+            10,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1628,14 +1622,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:14:58.686768786"
+        "timestamp": "2025-08-08T11:18:29.46107784"
     },
     "stub test fusioncatcher trim": {
         "content": [
-            12,
+            11,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1709,14 +1703,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T02:16:14.659256975"
+        "timestamp": "2025-08-08T11:19:01.10521868"
     },
     "stub test all": {
         "content": [
-            37,
+            35,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -2188,9 +2182,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-07T10:38:44.804072"
+        "timestamp": "2025-08-08T11:15:49.690844884"
     }
 }
\ No newline at end of file

From 6d7e101008739ecb99357819c681cbddc7db11d6 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 11:38:41 +0000
Subject: [PATCH 753/887] test: update test stub bam

---
 tests/test_stub_bam.nf.test.snap | 162 +++++++++++++++++++++++--------
 1 file changed, 120 insertions(+), 42 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 6959c0f10..439a583a4 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -188,10 +188,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:21:00.500662919"
+        "timestamp": "2025-08-08T11:34:21.840598583"
     },
     "stub test stringtie": {
         "content": [
@@ -310,14 +310,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:22:15.713895494"
+        "timestamp": "2025-08-08T11:34:57.146117646"
     },
     "stub test arriba": {
         "content": [
-            15,
+            14,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -325,9 +325,6 @@
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
@@ -425,14 +422,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:11:10.337479886"
+        "timestamp": "2025-08-08T11:31:27.759259528"
     },
     "stub test fusioninspector": {
         "content": [
-            24,
+            23,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -785,10 +782,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-07T15:10:07.731105"
+        "timestamp": "2025-08-08T11:38:28.107838971"
     },
     "stub test salmon": {
         "content": [
@@ -899,20 +896,104 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:33:59.679716364"
+        "timestamp": "2025-08-08T11:37:16.616916274"
     },
     "stub test ctatsplicing": {
         "content": [
-            0,
+            17,
             {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
+                "CTATSPLICING_PREPGENOMELIB": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "CTATSPLICING_STARTOCANCERINTRONS": {
+                    "ctat-splicing": "0.0.2"
+                },
+                "FASTQC": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION_BUILD": {
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
+                "ctatsplicing",
+                "ctatsplicing/test.cancer.introns",
+                "ctatsplicing/test.cancer.introns.prelim",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
+                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
+                "ctatsplicing/test.chckpts",
+                "ctatsplicing/test.ctat-splicing.igv.html",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
+                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
+                "ctatsplicing/test.igv.tracks",
+                "ctatsplicing/test.introns",
+                "ctatsplicing/test.introns.for_IGV.bed",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
@@ -1028,14 +1109,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:11:35.363568093"
+        "timestamp": "2025-08-08T11:32:37.072437849"
     },
     "stub test fusionreport": {
         "content": [
-            21,
+            19,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1043,9 +1124,6 @@
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_DOWNLOAD": {
-                    "arriba_download": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
@@ -1258,10 +1336,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:31:36.419602575"
+        "timestamp": "2025-08-08T11:36:12.01932932"
     },
     "stub test fastp": {
         "content": [
@@ -1357,14 +1435,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:32:51.98792128"
+        "timestamp": "2025-08-08T11:36:44.624772922"
     },
     "stub test fusioncatcher": {
         "content": [
-            14,
+            13,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1448,14 +1526,14 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-06T03:13:18.315049907"
+        "timestamp": "2025-08-08T11:33:11.268038905"
     },
     "stub test all": {
         "content": [
-            36,
+            34,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1903,9 +1981,9 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "25.04.2"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-07T13:56:14.46501"
+        "timestamp": "2025-08-08T11:30:49.983961991"
     }
 }
\ No newline at end of file

From 991e3554c697bb4e178c0804385981f95008448b Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 11:38:50 +0000
Subject: [PATCH 754/887] docs: update changelog

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c586d3f46..d56a8257b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -111,6 +111,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix fusionreport singularity container [#713](https://github.com/nf-core/rnafusion/pull/713)
 - Fix CTAT-SPLICING output when no cancer introns were found [#722](https://github.com/nf-core/rnafusion/pull/722)
 - Update VCF_COLLECT script to adapt to transcript_version not being an entry in fusioninspector gtf anymore [#726](https://github.com/nf-core/rnafusion/pull/726)
+- Fix rRNA detection and make it more customizable with nf-core modules [#736](https://github.com/nf-core/rnafusion/pull/736)
 
 ### Removed
 
@@ -122,6 +123,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed subworkflow with less than two modules: `STARFUSION_WORKFLOW` [#707](https://github.com/nf-core/rnafusion/pull/707)
 - Removed subworkflow with less than two modules: `CTATSPLICING_WORKFLOW` [#704](https://github.com/nf-core/rnafusion/pull/704)
 - Removed subworkflow with less than two modules: `FUSIONREPORT_WORKFLOW` [#721](https://github.com/nf-core/rnafusion/pull/721)
+- Removed local module `GET_RRNA_TRANSCRIPTS` [#736](https://github.com/nf-core/rnafusion/pull/736)
 
 ### Parameters
 

From f9f85530ab9336074572664a99eed830f486543e Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 11:41:46 +0000
Subject: [PATCH 755/887] chore: remove unused arriba download

---
 modules.json | 5 -----
 1 file changed, 5 deletions(-)

diff --git a/modules.json b/modules.json
index 28bd2ce93..1ab893088 100644
--- a/modules.json
+++ b/modules.json
@@ -20,11 +20,6 @@
                         "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
                         "installed_by": ["modules"]
                     },
-                    "arriba/download": {
-                        "branch": "master",
-                        "git_sha": "98bd7be6d2191b0d5900ee698d91b52eeab90311",
-                        "installed_by": ["modules"]
-                    },
                     "bioawk": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",

From 4ed4ab392a5971cd9e74f3e5e4f991b050847d4e Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 12:20:40 +0000
Subject: [PATCH 756/887] chore: replace local arriba visualization for nf-core

---
 .../arriba/visualisation/environment.yml      |   8 -
 modules/local/arriba/visualisation/meta.yml   |  54 -----
 .../arriba/visualisation/tests/main.nf.test   |  83 --------
 .../arriba/visualisation/environment.yml      |   6 +
 .../arriba/visualisation/main.nf              |  14 +-
 modules/nf-core/arriba/visualisation/meta.yml |  99 ++++++++++
 .../arriba/visualisation/tests/main.nf.test   | 184 ++++++++++++++++++
 .../visualisation/tests/main.nf.test.snap     |   8 +-
 .../visualisation/tests/nextflow.config       |  10 +
 9 files changed, 310 insertions(+), 156 deletions(-)
 delete mode 100644 modules/local/arriba/visualisation/environment.yml
 delete mode 100644 modules/local/arriba/visualisation/meta.yml
 delete mode 100644 modules/local/arriba/visualisation/tests/main.nf.test
 create mode 100644 modules/nf-core/arriba/visualisation/environment.yml
 rename modules/{local => nf-core}/arriba/visualisation/main.nf (75%)
 create mode 100644 modules/nf-core/arriba/visualisation/meta.yml
 create mode 100644 modules/nf-core/arriba/visualisation/tests/main.nf.test
 rename modules/{local => nf-core}/arriba/visualisation/tests/main.nf.test.snap (78%)
 create mode 100644 modules/nf-core/arriba/visualisation/tests/nextflow.config

diff --git a/modules/local/arriba/visualisation/environment.yml b/modules/local/arriba/visualisation/environment.yml
deleted file mode 100644
index b30731312..000000000
--- a/modules/local/arriba/visualisation/environment.yml
+++ /dev/null
@@ -1,8 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-dependencies:
-  - bioconda::arriba=2.5.0
-  - conda-forge::wget=1.21.4
diff --git a/modules/local/arriba/visualisation/meta.yml b/modules/local/arriba/visualisation/meta.yml
deleted file mode 100644
index a7418ca24..000000000
--- a/modules/local/arriba/visualisation/meta.yml
+++ /dev/null
@@ -1,54 +0,0 @@
-name: arriba_visualisation
-description: Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data.
-keywords:
-  - visualisation
-  - arriba
-tools:
-  - arriba:
-      description: Fast and accurate gene fusion detection from RNA-Seq data
-      homepage: https://github.com/suhrig/arriba
-      documentation: https://arriba.readthedocs.io/en/latest/
-      tool_dev_url: https://github.com/suhrig/arriba
-      doi: "10.1101/gr.257246.119"
-      licence: ["MIT"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - bam:
-      type: file
-      description: BAM/CRAM/SAM file
-      pattern: "*.{bam,cram,sam}"
-  - bai:
-      type: file
-      description: BAMindex file
-      pattern: "*.{bai}"
-  - fusions:
-      type: file
-      description: Arriba fusions file
-      pattern: "*.{tsv}"
-  - gtf:
-      type: file
-      description: Annotation GTF file
-      pattern: "*.{gtf}"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - pdf:
-      type: file
-      description: File contains fusions visualisation
-      pattern: "*.{pdf}"
-
-authors:
-  - "@rannick"
diff --git a/modules/local/arriba/visualisation/tests/main.nf.test b/modules/local/arriba/visualisation/tests/main.nf.test
deleted file mode 100644
index bdf5269e0..000000000
--- a/modules/local/arriba/visualisation/tests/main.nf.test
+++ /dev/null
@@ -1,83 +0,0 @@
-nextflow_process {
-
-    name "Test Process ARRIBA_VISUALISATION"
-    script "../main.nf"
-    process "ARRIBA_VISUALISATION"
-    tag "modules"
-    tag "modules_nfcore"
-    tag "arriba"
-    tag "arriba/visualisation"
-
-    test("ARRIBA_VISUALISATION") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                    [],
-                    [],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv", checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'test_gtf' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'test_protein_domains' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true)
-                ]
-                input[3] = [
-                    [ id:'test_cytobands' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert file(process.out.pdf[0][1]).name },
-                { assert snapshot(process.out.versions).match("versions") }
-            )
-        }
-    }
-
-    test("ARRIBA_VISUALISATION stub") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam", checkIfExists: true),
-                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai", checkIfExists: true),
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv", checkIfExists: true)
-                ]
-                input[1] = [
-                    [ id:'test_gtf' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true)
-                ]
-                input[2] = [
-                    [ id:'test_protein_domains' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true)
-                ]
-                input[3] = [
-                    [ id:'test_cytobands' ],
-                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out).match() }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/arriba/visualisation/environment.yml b/modules/nf-core/arriba/visualisation/environment.yml
new file mode 100644
index 000000000..fa3366764
--- /dev/null
+++ b/modules/nf-core/arriba/visualisation/environment.yml
@@ -0,0 +1,6 @@
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::arriba=2.5.0
+  - conda-forge::wget=1.21.4
diff --git a/modules/local/arriba/visualisation/main.nf b/modules/nf-core/arriba/visualisation/main.nf
similarity index 75%
rename from modules/local/arriba/visualisation/main.nf
rename to modules/nf-core/arriba/visualisation/main.nf
index c5fed05a0..a1e2ce8d6 100644
--- a/modules/local/arriba/visualisation/main.nf
+++ b/modules/nf-core/arriba/visualisation/main.nf
@@ -8,24 +8,24 @@ process ARRIBA_VISUALISATION {
         'community.wave.seqera.io/library/arriba_wget:a3e48cf793a0b654' }"
 
     input:
-    tuple val(meta), path(bam), path(bai), path(fusions)
+    tuple val(meta) , path(bam), path(bai), path(fusions)
     tuple val(meta2), path(gtf)
     tuple val(meta3), path(protein_domains)
     tuple val(meta4), path(cytobands)
 
     output:
-    tuple val(meta), path("*.pdf")          , emit: pdf
-    path "versions.yml"                     , emit: versions
+    tuple val(meta), path("*.pdf"), emit: pdf
+    path "versions.yml"           , emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def arg_cytobands = cytobands ? " --cytobands=$cytobands" : ""
-    def arg_alignment = bam ? " --alignments=$bam" : ""
+    def args                = task.ext.args   ?: ''
+    def arg_cytobands       = cytobands       ? " --cytobands=$cytobands"           : ""
+    def arg_alignment       = bam             ? " --alignments=$bam"                : ""
     def arg_protein_domains = protein_domains ? "--proteinDomains=$protein_domains" : ""
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    def prefix              = task.ext.prefix ?: "${meta.id}"
     """
     draw_fusions.R \\
         --fusions=$fusions \\
diff --git a/modules/nf-core/arriba/visualisation/meta.yml b/modules/nf-core/arriba/visualisation/meta.yml
new file mode 100644
index 000000000..fa2a20683
--- /dev/null
+++ b/modules/nf-core/arriba/visualisation/meta.yml
@@ -0,0 +1,99 @@
+name: arriba_visualisation
+description: Arriba is a command-line tool for the detection of gene fusions
+  from RNA-Seq data.
+keywords:
+  - visualisation
+  - arriba
+  - fusion
+  - RNA-Seq
+tools:
+  - arriba:
+      description: Fast and accurate gene fusion detection from RNA-Seq data
+      homepage: https://github.com/suhrig/arriba
+      documentation: https://arriba.readthedocs.io/en/latest/
+      tool_dev_url: https://github.com/suhrig/arriba
+      doi: "10.1101/gr.257246.119"
+      licence: ["MIT"]
+      identifier: biotools:Arriba
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+        ontologies:
+          - edam: "http://edamontology.org/format_2572" # BAM
+          - edam: "http://edamontology.org/format_3462" # CRAM
+          - edam: "http://edamontology.org/format_2573" # SAM
+    - bai:
+        type: file
+        description: BAM index file
+        pattern: "*.bai"
+        ontologies:
+          - edam: "http://edamontology.org/format_3327" # BAI
+    - fusions:
+        type: file
+        description: Arriba fusions file
+        pattern: "*.tsv"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - gtf:
+        type: file
+        description: Annotation GTF file
+        pattern: "*.gtf"
+        ontologies:
+          - edam: "http://edamontology.org/format_2306" # GTF
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - protein_domains:
+        type: file
+        description: Protein domain annotation files
+        pattern: "*.gff3"
+        ontologies:
+          - edam: "http://edamontology.org/format_1975" # GFF3
+  - - meta4:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - cytobands:
+        type: file
+        description: Coordinates of the Giemsa staining bands needed to draw
+          ideograms
+        pattern: ".tsv"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+output:
+  pdf:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.pdf":
+          type: file
+          description: File contains fusions visualisation
+          pattern: "*.pdf"
+          ontologies:
+            - edam: "http://edamontology.org/format_3508" # PDF
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: "http://edamontology.org/format_3750" # YAML
+authors:
+  - "@rannick"
diff --git a/modules/nf-core/arriba/visualisation/tests/main.nf.test b/modules/nf-core/arriba/visualisation/tests/main.nf.test
new file mode 100644
index 000000000..33b274c01
--- /dev/null
+++ b/modules/nf-core/arriba/visualisation/tests/main.nf.test
@@ -0,0 +1,184 @@
+nextflow_process {
+
+    name "Test Process ARRIBA_VISUALISATION"
+    script "../main.nf"
+    process "ARRIBA_VISUALISATION"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "arriba"
+    tag "arriba/visualisation"
+    tag "star/align"
+    tag "star/genomegenerate"
+    tag "arriba/arriba"
+    tag "arriba/download"
+
+    test("ARRIBA_VISUALISATION") {
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [],
+                    [],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv", checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_gtf' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'test_protein_domains' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true)
+                ]
+                input[3] = [
+                    [ id:'test_cytobands' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.pdf[0][1]).length()) },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("ARRIBA_VISUALISATION - arriba test") {
+        config "./nextflow.config"
+
+        setup{
+
+            run("STAR_GENOMEGENERATE") {
+                script '../../../star/genomegenerate/main.nf'
+                process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+                }
+            }
+
+            run("STAR_ALIGN") {
+                script '../../../star/align/main.nf'
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test', single_end:false ], // meta map
+                        [
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                        ]
+                    ])
+                    input[1] = STAR_GENOMEGENERATE.out.index
+                    input[2] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    input[3] = false
+                    input[4] = 'illumina'
+                    input[5] = false
+                    """
+                }
+            }
+
+            run("ARRIBA_ARRIBA") {
+                script '../../arriba/main.nf'
+                process {
+                    """
+                    input[0] = STAR_ALIGN.out.bam
+                    input[1] = Channel.of([
+                        [ id:'test_fasta' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                    ])
+                    input[2] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    input[3] = []
+                    input[4] = []
+                    input[5] = []
+                    input[6] = []
+                    """
+                }
+            }
+            run("ARRIBA_DOWNLOAD") {
+                script '../../download/main.nf'
+                process {
+                """
+                input[0] = 'GRCh38'
+                """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = ARRIBA_ARRIBA.out.fusions.map { meta, fusions -> [ meta, [], [], fusions ] }
+                input[1] = [
+                    [ id:'test_gtf' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true)
+                ]
+                input[2] = ARRIBA_DOWNLOAD.out.protein_domains.map { protein_domains -> [[ id:'test_protein_domains' ], protein_domains] }
+                input[3] = ARRIBA_DOWNLOAD.out.cytobands.map { cytobands -> [[ id:'test_cytobands' ], cytobands] }
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(file(process.out.pdf[0][1]).length()) },
+                { assert snapshot(process.out.versions).match("versions") }
+            )
+        }
+    }
+
+    test("ARRIBA_VISUALISATION stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam", checkIfExists: true),
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai", checkIfExists: true),
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/test_fastqs.arriba.fusions.tsv", checkIfExists: true)
+                ]
+                input[1] = [
+                    [ id:'test_gtf' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/ensembl/Homo_sapiens.GRCh38.102.chr4.gtf", checkIfExists: true)
+                ]
+                input[2] = [
+                    [ id:'test_protein_domains' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3", checkIfExists: true)
+                ]
+                input[3] = [
+                    [ id:'test_cytobands' ],
+                    file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv", checkIfExists: true)
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/local/arriba/visualisation/tests/main.nf.test.snap b/modules/nf-core/arriba/visualisation/tests/main.nf.test.snap
similarity index 78%
rename from modules/local/arriba/visualisation/tests/main.nf.test.snap
rename to modules/nf-core/arriba/visualisation/tests/main.nf.test.snap
index 37b4aead2..c46b1b0e1 100644
--- a/modules/local/arriba/visualisation/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/visualisation/tests/main.nf.test.snap
@@ -9,7 +9,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-16T09:51:22.731321"
+        "timestamp": "2025-07-22T14:26:51.236386176"
     },
     "ARRIBA_VISUALISATION stub": {
         "content": [
@@ -20,7 +20,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "1": [
@@ -32,7 +32,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
                 "versions": [
@@ -44,6 +44,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-16T09:51:53.819687"
+        "timestamp": "2025-07-21T14:05:27.049968298"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/arriba/visualisation/tests/nextflow.config b/modules/nf-core/arriba/visualisation/tests/nextflow.config
new file mode 100644
index 000000000..595437321
--- /dev/null
+++ b/modules/nf-core/arriba/visualisation/tests/nextflow.config
@@ -0,0 +1,10 @@
+process {
+    withName: STAR_GENOMEGENERATE {
+        ext.args = '--genomeSAindexNbases 9'
+    }
+
+    withName: STAR_ALIGN {
+        ext.args = '--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50'
+    }
+}
+

From 82ab0f40e2ac5412ef31d12b85458ca95f418560 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 12:21:36 +0000
Subject: [PATCH 757/887] chore: replace local ctatsplicing for nf-core

---
 .../local/ctatsplicing/prepgenomelib/main.nf  |  44 ---
 .../prepgenomelib/tests/main.nf.test          | 159 ----------
 .../prepgenomelib/tests/main.nf.test.snap     | 194 ------------
 .../startocancerintrons/tests/main.nf.test    |  69 ----
 .../ctatsplicing/prepgenomelib/main.nf        | 129 ++++++++
 .../ctatsplicing/prepgenomelib/meta.yml       |  57 ++++
 .../prepgenomelib/tests/main.nf.test          | 109 +++++++
 .../prepgenomelib/tests/main.nf.test.snap     | 299 ++++++++++++++++++
 .../ctatsplicing/startocancerintrons/main.nf  |  43 ++-
 .../ctatsplicing/startocancerintrons/meta.yml | 189 +++++++++++
 .../startocancerintrons/tests/main.nf.test    | 186 +++++++++++
 .../tests/main.nf.test.snap                   | 177 +++++++----
 .../startocancerintrons/tests/nextflow.config |  33 ++
 13 files changed, 1139 insertions(+), 549 deletions(-)
 delete mode 100644 modules/local/ctatsplicing/prepgenomelib/main.nf
 delete mode 100644 modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
 delete mode 100644 modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
 delete mode 100644 modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test
 create mode 100644 modules/nf-core/ctatsplicing/prepgenomelib/main.nf
 create mode 100644 modules/nf-core/ctatsplicing/prepgenomelib/meta.yml
 create mode 100644 modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test
 create mode 100644 modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
 rename modules/{local => nf-core}/ctatsplicing/startocancerintrons/main.nf (73%)
 create mode 100644 modules/nf-core/ctatsplicing/startocancerintrons/meta.yml
 create mode 100644 modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test
 rename modules/{local => nf-core}/ctatsplicing/startocancerintrons/tests/main.nf.test.snap (55%)
 create mode 100644 modules/nf-core/ctatsplicing/startocancerintrons/tests/nextflow.config

diff --git a/modules/local/ctatsplicing/prepgenomelib/main.nf b/modules/local/ctatsplicing/prepgenomelib/main.nf
deleted file mode 100644
index 04f09e1aa..000000000
--- a/modules/local/ctatsplicing/prepgenomelib/main.nf
+++ /dev/null
@@ -1,44 +0,0 @@
-process CTATSPLICING_PREPGENOMELIB {
-    tag "$meta.id"
-    label 'process_single'
-
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://data.broadinstitute.org/Trinity/CTAT_SINGULARITY/CTAT-SPLICING/ctat_splicing.v0.0.2.simg' :
-        'docker.io/trinityctat/ctat_splicing:0.0.2' }"
-
-    input:
-    tuple val(meta), path(genome_lib)
-    path(cancer_intron_tsv)
-
-    output:
-    tuple val(meta), path(genome_lib, includeInputs:true), emit: reference
-    path "versions.yml"                                  , emit: versions
-
-    script:
-    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    /usr/local/src/CTAT-SPLICING/prep_genome_lib/ctat-splicing-lib-integration.py \\
-        --cancer_introns_tsv cancer_introns.*.tsv.gz \\
-        --genome_lib_dir $genome_lib
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ctat-splicing: $VERSION
-    END_VERSIONS
-    """
-
-    stub:
-    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    """
-    touch $genome_lib/refGene.bed
-    echo | gzip > $genome_lib/refGene.sort.bed.gz
-    touch $genome_lib/refGene.sort.bed.gz.tbi
-    mkdir $genome_lib/cancer_splicing_lib
-    touch $genome_lib/cancer_splicing_lib/cancer_splicing.idx
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ctat-splicing: $VERSION
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
deleted file mode 100644
index 86886fd08..000000000
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test
+++ /dev/null
@@ -1,159 +0,0 @@
-nextflow_process {
-
-    name "Test Process CTATSPLICING_PREPGENOMELIB"
-    script "../main.nf"
-    process "CTATSPLICING_PREPGENOMELIB"
-
-    setup {
-        run("STARFUSION_BUILD") {
-            script "../../../../nf-core/starfusion/build/main.nf"
-            process {
-                """
-                input[0] = [
-                    [ id:'minigenome_fasta' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
-                ]
-                input[1] = [
-                    [ id:'minigenome_gtf' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
-                ]
-
-                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
-                input[3] = "homo_sapiens"
-                input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
-                input[5] = [
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
-                ]
-                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
-                """
-            }
-        }
-    }
-
-    test("test") {
-
-        when {
-            process {
-                """
-                input[0] = STARFUSION_BUILD.out.reference
-                input[1] = [
-                    file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
-                ]
-                """
-            }
-        }
-
-        then {
-            def outputDir = file(process.out.reference[0][1])
-            assert snapshot(
-                path(process.out.reference[0][1]).resolve("AnnotFilterRule.pm"),
-                file("$outputDir/blast_pairs.dat.gz").name,
-                file("$outputDir/blast_pairs.idx").name,
-                file("$outputDir/__chkpts/annotfiltrule_cp.ok").name,
-                file("$outputDir/__chkpts/blast_pairs.idx.ok").name,
-                file("$outputDir/__chkpts/cp_gene_blast_pairs.ok").name,
-                file("$outputDir/__chkpts/cp_pfam_dat.ok").name,
-                file("$outputDir/__chkpts/cp_ref_annot_cdna.ok").name,
-                file("$outputDir/__chkpts/fusion_annot_lib.cp.ok").name,
-                file("$outputDir/__chkpts/_fusion_annot_lib.idx.ok").name,
-                file("$outputDir/__chkpts/index_pfam_hits.ok").name,
-                file("$outputDir/__chkpts/index_ref_annot_cdna.ok").name,
-                file("$outputDir/__chkpts/makeblastdb.ok").name,
-                file("$outputDir/__chkpts/mm2_genome_idx.ok").name,
-                file("$outputDir/__chkpts/mm2.splice_bed.ok").name,
-                file("$outputDir/__chkpts/_prot_info_db.ok").name,
-                file("$outputDir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok").name,
-                file("$outputDir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok").name,
-                file("$outputDir/__chkpts/ref_annot.gtf.gene_spans.ok").name,
-                file("$outputDir/__chkpts/ref_annot.gtf.mini.sortu.ok").name,
-                file("$outputDir/__chkpts/ref_annot.gtf.ok").name,
-                file("$outputDir/__chkpts/ref_genome_fai.ok").name,
-                file("$outputDir/__chkpts/ref_genome.fa.ok").name,
-                file("$outputDir/__chkpts/trans.blast.dat.cp.ok").name,
-                file("$outputDir/__chkpts/trans.blast.dat.index.ok").name,
-                file("$outputDir/__chkpts/validate_ctat_genome_lib.ok").name,
-                path(process.out.reference[0][1]).resolve("fusion_annot_lib.gz"),
-                file("$outputDir/fusion_annot_lib.idx").name,
-                file("$outputDir/pfam_domains.dbm").name,
-                file("$outputDir/PFAM.domtblout.dat.gz").name,
-                file("$outputDir/ref_annot.cdna.fa").name,
-                file("$outputDir/ref_annot.cdna.fa.idx").name,
-                file("$outputDir/ref_annot.cds").name,
-                file("$outputDir/ref_annot.cdsplus.fa").name,
-                file("$outputDir/ref_annot.cdsplus.fa.idx").name,
-                path(process.out.reference[0][1]).resolve("ref_annot.gtf"),
-                file("$outputDir/ref_annot.gtf.gene_spans").name,
-                path(process.out.reference[0][1]).resolve("ref_annot.gtf.mini.sortu"),
-                path(process.out.reference[0][1]).resolve("ref_annot.gtf.mm2.splice.bed"),
-                file("$outputDir/ref_annot.pep").name,
-                file("$outputDir/ref_annot.prot_info.dbm").name,
-                path(process.out.reference[0][1]).resolve("ref_genome.fa"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.fai"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.mm2"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.ndb"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.nhr"),
-                file("$outputDir/ref_genome.fa.nin").name,
-                file("$outputDir/ref_genome.fa.njs").name,
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.not"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.nsq"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.ntf"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.nto"),
-                file("$outputDir/ref_genome.fa.star.idx").name,
-                file("$outputDir/ref_genome.fa.star.idx/build.ok").name,
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrLength.txt"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrNameLength.txt"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrName.txt"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/chrStart.txt"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonGeTrInfo.tab"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/exonInfo.tab"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/geneInfo.tab"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/Genome"),
-                file("$outputDir/ref_genome.fa.star.idx/genomeParameters.txt").name,
-                file("$outputDir/ref_genome.fa.star.idx/Log.out").name,
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SA"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/SAindex"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbInfo.txt"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/sjdbList.out.tab"),
-                path(process.out.reference[0][1]).resolve("ref_genome.fa.star.idx/transcriptInfo.tab"),
-                path(process.out.reference[0][1]).resolve("trans.blast.align_coords.align_coords.dat"),
-                file("$outputDir/trans.blast.align_coords.align_coords.dbm").name,
-                path(process.out.reference[0][1]).resolve("trans.blast.dat.gz"),
-                path(process.out.reference[0][1]).resolve("refGene.bed").exists(),
-                path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz"),
-                path(process.out.reference[0][1]).resolve("refGene.sort.bed.gz.tbi"),
-                path(process.out.reference[0][1]).resolve("cancer_splicing_lib/cancer_splicing.idx").exists(),
-                process.out.version
-                ).match()
-        }
-    }
-    test("test - stub") {
-
-        options "-stub"
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-            process {
-                """
-                input[0] = STARFUSION_BUILD.out.reference
-                input[1] = [
-                    file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
-            )
-        }
-    }
-}
diff --git a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
deleted file mode 100644
index 80739989c..000000000
--- a/modules/local/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
+++ /dev/null
@@ -1,194 +0,0 @@
-{
-    "test": {
-        "content": [
-            "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
-            "blast_pairs.dat.gz",
-            "blast_pairs.idx",
-            "annotfiltrule_cp.ok",
-            "blast_pairs.idx.ok",
-            "cp_gene_blast_pairs.ok",
-            "cp_pfam_dat.ok",
-            "cp_ref_annot_cdna.ok",
-            "fusion_annot_lib.cp.ok",
-            "_fusion_annot_lib.idx.ok",
-            "index_pfam_hits.ok",
-            "index_ref_annot_cdna.ok",
-            "makeblastdb.ok",
-            "mm2_genome_idx.ok",
-            "mm2.splice_bed.ok",
-            "_prot_info_db.ok",
-            "ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-            "ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-            "ref_annot.gtf.gene_spans.ok",
-            "ref_annot.gtf.mini.sortu.ok",
-            "ref_annot.gtf.ok",
-            "ref_genome_fai.ok",
-            "ref_genome.fa.ok",
-            "trans.blast.dat.cp.ok",
-            "trans.blast.dat.index.ok",
-            "validate_ctat_genome_lib.ok",
-            "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
-            "fusion_annot_lib.idx",
-            "pfam_domains.dbm",
-            "PFAM.domtblout.dat.gz",
-            "ref_annot.cdna.fa",
-            "ref_annot.cdna.fa.idx",
-            "ref_annot.cds",
-            "ref_annot.cdsplus.fa",
-            "ref_annot.cdsplus.fa.idx",
-            "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
-            "ref_annot.gtf.gene_spans",
-            "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
-            "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
-            "ref_annot.pep",
-            "ref_annot.prot_info.dbm",
-            "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
-            "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
-            "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
-            "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
-            "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
-            "ref_genome.fa.nin",
-            "ref_genome.fa.njs",
-            "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
-            "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
-            "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
-            "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
-            "ref_genome.fa.star.idx",
-            "build.ok",
-            "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
-            "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
-            "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
-            "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
-            "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
-            "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
-            "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
-            "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
-            "genomeParameters.txt",
-            "Log.out",
-            "SA:md5,7dd9083264be9c6a2194d990bc10d237",
-            "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
-            "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
-            "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
-            "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
-            "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
-            "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
-            "trans.blast.align_coords.align_coords.dbm",
-            "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128",
-            true,
-            "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
-            "refGene.sort.bed.gz.tbi:md5,6dd8cd94a5cd10699fe8445a70c5b232",
-            true,
-            null
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-26T20:17:06.519409"
-    },
-    "test - stub": {
-        "content": [
-            {
-                "reference": [
-                    [
-                        {
-                            "id": "minigenome_fasta"
-                        },
-                        [
-                            "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "PFAM.domtblout.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            [
-                                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                            ],
-                            "blast_pairs.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            [
-                                "cancer_splicing.idx:md5,d41d8cd98f00b204e9800998ecf8427e"
-                            ],
-                            "fusion_annot_lib.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "refGene.sort.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
-                            "refGene.sort.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            [
-                                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
-                            ],
-                            "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "trans.blast.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
-                        ]
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,8175cd19f5d5b8b2214cd948120d7524"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-25T15:18:45.370275"
-    }
-}
\ No newline at end of file
diff --git a/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test b/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test
deleted file mode 100644
index dad961c47..000000000
--- a/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test
+++ /dev/null
@@ -1,69 +0,0 @@
-nextflow_process {
-
-    name "Test Process CTATSPLICING_STARTOCANCERINTRONS"
-    script "../main.nf"
-    process "CTATSPLICING_STARTOCANCERINTRONS"
-    options "-stub"
-
-    test("test without BAM") {
-
-        when {
-            params {
-                outdir = "tests/results"
-            }
-            process {
-                """
-                input[0] = [
-                    [id:"test"],
-                    file("test.SJ.out.tab"),
-                    file("test.Chimeric.out.junctions"),
-                    [],
-                    []
-                ]
-                input[1] = [
-                    [id:"reference"],
-                    file("ctat_genome_lib")
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
-            )
-        }
-    }
-
-    test("test with BAM") {
-
-        when {
-            params {
-                outdir = "tests/results"
-            }
-            process {
-                """
-                input[0] = [
-                    [id:"test"],
-                    file("test.SJ.out.tab"),
-                    file("test.Chimeric.out.junctions"),
-                    file("test.Aligned.sortedByCoord.out.bam"),
-                    []
-                ]
-                input[1] = [
-                    [id:"reference"],
-                    file("ctat_genome_lib")
-                ]
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert process.success },
-                { assert snapshot(process.out.findAll { key, value -> !key.isNumber() }).match() }
-            )
-        }
-    }
-}
diff --git a/modules/nf-core/ctatsplicing/prepgenomelib/main.nf b/modules/nf-core/ctatsplicing/prepgenomelib/main.nf
new file mode 100644
index 000000000..3a3539822
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/prepgenomelib/main.nf
@@ -0,0 +1,129 @@
+process CTATSPLICING_PREPGENOMELIB {
+    tag "$meta.id"
+    label 'process_single'
+    stageInMode 'copy'
+
+    container "nf-core/ctatsplicing:0.0.3"
+
+    input:
+    tuple val(meta), path(genome_lib)
+    path(cancer_intron_tsv)
+
+    output:
+    tuple val(meta), path(genome_lib), emit: reference
+    path "versions.yml"              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "CTATSPLICING_PREPGENOMELIB module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    /usr/local/src/CTAT-SPLICING/prep_genome_lib/ctat-splicing-lib-integration.py \\
+        --cancer_introns_tsv $cancer_intron_tsv \\
+        --genome_lib_dir $genome_lib
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ctatsplicing: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    mkdir -p $genome_lib/
+    mkdir -p $genome_lib/ref_genome.fa.star.idx
+    mkdir -p $genome_lib/__chkpts
+    mkdir -p $genome_lib/cancer_splicing_lib
+    touch $genome_lib/ref_genome.fa.star.idx/chrName.txt
+    touch $genome_lib/ref_genome.fa.star.idx/exonInfo.tab
+    touch $genome_lib/ref_genome.fa.star.idx/SAindex
+    touch $genome_lib/ref_genome.fa.star.idx/Genome
+    touch $genome_lib/ref_genome.fa.star.idx/geneInfo.tab
+    touch $genome_lib/ref_genome.fa.star.idx/sjdbList.out.tab
+    touch $genome_lib/ref_genome.fa.star.idx/sjdbInfo.txt
+    touch $genome_lib/ref_genome.fa.star.idx/transcriptInfo.tab
+    touch $genome_lib/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab
+    touch $genome_lib/ref_genome.fa.star.idx/build.ok
+    touch $genome_lib/ref_genome.fa.star.idx/chrLength.txt
+    touch $genome_lib/ref_genome.fa.star.idx/Log.out
+    touch $genome_lib/ref_genome.fa.star.idx/genomeParameters.txt
+    touch $genome_lib/ref_genome.fa.star.idx/chrStart.txt
+    touch $genome_lib/ref_genome.fa.star.idx/SA
+    touch $genome_lib/ref_genome.fa.star.idx/chrNameLength.txt
+    touch $genome_lib/ref_genome.fa.star.idx/exonGeTrInfo.tab
+    touch $genome_lib/ref_annot.cds
+    touch $genome_lib/ref_annot.gtf.gene_spans
+    touch $genome_lib/pfam_domains.dbm
+    touch $genome_lib/ref_annot.cdsplus.fa.idx
+    touch $genome_lib/ref_genome.fa.ndb
+    touch $genome_lib/ref_genome.fa.nin
+    touch $genome_lib/ref_genome.fa
+    touch $genome_lib/ref_annot.prot_info.dbm
+    echo | gzip > $genome_lib/PFAM.domtblout.dat.gz
+    touch $genome_lib/trans.blast.align_coords.align_coords.dat
+    touch $genome_lib/fusion_annot_lib.idx
+    touch $genome_lib/ref_annot.pep
+    touch $genome_lib/__chkpts/trans.blast.dat.cp.ok
+    touch $genome_lib/__chkpts/cp_ref_annot_cdna.ok
+    touch $genome_lib/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok
+    touch $genome_lib/__chkpts/annotfiltrule_cp.ok
+    touch $genome_lib/__chkpts/validate_ctat_genome_lib.ok
+    touch $genome_lib/__chkpts/cp_pfam_dat.ok
+    touch $genome_lib/__chkpts/fusion_annot_lib.cp.ok
+    touch $genome_lib/__chkpts/ref_genome.fa.ok
+    touch $genome_lib/__chkpts/cp_gene_blast_pairs.ok
+    touch $genome_lib/__chkpts/mm2_genome_idx.ok
+    touch $genome_lib/__chkpts/trans.blast.dat.index.ok
+    touch $genome_lib/__chkpts/ref_genome_fai.ok
+    touch $genome_lib/__chkpts/index_ref_annot_cdna.ok
+    touch $genome_lib/__chkpts/ref_annot.gtf.ok
+    touch $genome_lib/__chkpts/blast_pairs.idx.ok
+    touch $genome_lib/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok
+    touch $genome_lib/__chkpts/_prot_info_db.ok
+    touch $genome_lib/__chkpts/_fusion_annot_lib.idx.ok
+    touch $genome_lib/__chkpts/index_pfam_hits.ok
+    touch $genome_lib/__chkpts/makeblastdb.ok
+    touch $genome_lib/__chkpts/ref_annot.gtf.gene_spans.ok
+    touch $genome_lib/__chkpts/mm2.splice_bed.ok
+    touch $genome_lib/__chkpts/ref_annot.gtf.mini.sortu.ok
+    touch $genome_lib/ref_genome.fa.ntf
+    touch $genome_lib/ref_genome.fa.nto
+    touch $genome_lib/ref_annot.gtf
+    touch $genome_lib/ref_annot.gtf.mm2.splice.bed
+    touch $genome_lib/ref_genome.fa.mm2
+    echo | gzip > $genome_lib/fusion_annot_lib.gz
+    touch $genome_lib/ref_annot.cdsplus.fa
+    touch $genome_lib/AnnotFilterRule.pm
+    echo | gzip > $genome_lib/trans.blast.dat.gz
+    touch $genome_lib/ref_genome.fa.nhr
+    touch $genome_lib/blast_pairs.idx
+    touch $genome_lib/ref_genome.fa.nsq
+    echo | gzip > $genome_lib/blast_pairs.dat.gz
+    touch $genome_lib/ref_annot.cdna.fa.idx
+    touch $genome_lib/ref_genome.fa.not
+    touch $genome_lib/ref_annot.cdna.fa
+    touch $genome_lib/ref_annot.gtf.mini.sortu
+    touch $genome_lib/trans.blast.align_coords.align_coords.dbm
+    touch $genome_lib/ref_genome.fa.njs
+    touch $genome_lib/ref_genome.fa.fai
+    touch $genome_lib/refGene.bed
+    echo | gzip > $genome_lib/refGene.sort.bed.gz
+    echo | gzip > $genome_lib/refGene.sort.bed.gz.tbi
+    touch $genome_lib/cancer_splicing_lib/cancer_splicing.idx
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        ctatsplicing: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/ctatsplicing/prepgenomelib/meta.yml b/modules/nf-core/ctatsplicing/prepgenomelib/meta.yml
new file mode 100644
index 000000000..1efecef75
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/prepgenomelib/meta.yml
@@ -0,0 +1,57 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "ctatsplicing_prepgenomelib"
+description: Reference preparation for CTAT-splicing
+long_description: |
+  This module prepares the CTAT genome library reference for splicing analysis by integrating cancer intron annotations.
+  It takes a CTAT genome library and a cancer intron TSV file as input, and outputs a modified genome library reference.
+  The output can be used in subsequent CTAT-splicing analyses to detect and annotate cancer splicing aberrations.
+  Make sure to provide the correct genome library and cancer intron data resource.
+keywords:
+  - splicing
+  - cancer
+  - rna
+  - rnaseq
+tools:
+  - "ctatsplicing":
+      description: "Detection and annotation of cancer splicing aberrations"
+      homepage: "https://github.com/TrinityCTAT/CTAT-SPLICING"
+      documentation: "https://github.com/TrinityCTAT/CTAT-SPLICING"
+      tool_dev_url: "https://github.com/TrinityCTAT/CTAT-SPLICING"
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - genome_lib:
+        type: file
+        description: CTAT genome library reference
+        pattern: "*"
+  - cancer_intron_tsv:
+      type: file
+      description: CTAT-splicing data resource supplement
+
+output:
+  reference:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. `[ id:'sample1', single_end:false ]`
+      - genome_lib:
+          type: file
+          description: Modified CTAT genome library reference
+          pattern: "*"
+  versions:
+    - "versions.yml":
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+
+authors:
+  - "@anoronh4"
+  - "@nvnieuwk"
+maintainers:
+  - "@anoronh4"
diff --git a/modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test b/modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test
new file mode 100644
index 000000000..b9fec4a72
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test
@@ -0,0 +1,109 @@
+nextflow_process {
+
+    name "Test Process CTATSPLICING_PREPGENOMELIB"
+    script "../main.nf"
+    process "CTATSPLICING_PREPGENOMELIB"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "ctatsplicing"
+    tag "ctatsplicing/prepgenomelib"
+    tag "starfusion/build"
+
+    test("homo_sapiens - reference") {
+        setup {
+            run("STARFUSION_BUILD") {
+                script "../../../starfusion/build/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'minigenome_fasta' ],
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
+                    ]
+                    input[1] = [
+                        [ id:'minigenome_gtf' ],
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
+                    ]
+                    input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                    input[3] = "homo_sapiens"
+                    input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                    input[5] = [
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                    ]
+                    input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = STARFUSION_BUILD.out.reference
+                input[1] = [
+                    file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
+                ]
+                """
+            }
+        }
+
+        then {
+            def stable_name    = getAllFilesFromDir(process.out.reference.get(0).get(1), includeDir: true)
+            def stable_files = getAllFilesFromDir(process.out.reference.get(0).get(1), includeDir: false, include: ['*','**/*'],
+                ignore: ['refGene.sort.bed.gz.tbi','**/refGene.sort.bed.gz.tbi', 'refGene.bed', '**/cancer_splicing.idx', 'ref_annot.c*',
+                    'PFAM.domtblout.dat.gz', 'blast_pairs*', 'fusion_annot_lib.idx', 'pfam_domains.dbm', 'ref_annot.gtf.gene_spans',
+                    'ref_annot.prot_info.dbm', 'ref_annot.pep', 'ref_genome.fa.nin', 'ref_genome.fa.njs', '**/Log.out', '**/genomeParameters.txt', 'trans.blast.align_coords.align_coords.dbm'])
+            def stable_content = stable_files.findAll { it.size() > 0 }
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        stable_name*.name,
+                        stable_content,
+                        process.out.versions
+                    ).match()
+                }
+            )
+
+        }
+    }
+
+    test("homo_sapiens - reference - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                new File('ctat_genome_lib_build_dir').mkdirs()
+                input[0] = [[id:"ctat_genome_lib_build_dir"],file("ctat_genome_lib_build_dir/")]
+                input[1] = [
+                    file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
+                ]
+                """
+            }
+        }
+
+        then {
+            def stable_name    = getAllFilesFromDir(process.out.reference.get(0).get(1), includeDir: true)
+            def stable_content = getAllFilesFromDir(process.out.reference.get(0).get(1), includeDir: false, include: ['*','**/*'], ignore: ['refGene.sort.bed.gz.tbi','**/refGene.sort.bed.gz.tbi','**/cancer_splicing.idx'])
+            assertAll(
+                { assert process.success },
+                {
+                    assert snapshot(
+                        stable_name*.name,
+                        stable_content,
+                        process.out.versions
+                    ).match()
+                }
+            )
+
+        }
+
+    }
+
+}
diff --git a/modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test.snap b/modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
new file mode 100644
index 000000000..8d801aa01
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test.snap
@@ -0,0 +1,299 @@
+{
+    "homo_sapiens - reference": {
+        "content": [
+            [
+                "minigenome_fasta_genome_lib_build_dir",
+                "AnnotFilterRule.pm",
+                "PFAM.domtblout.dat.gz",
+                "__chkpts",
+                "_fusion_annot_lib.idx.ok",
+                "_prot_info_db.ok",
+                "annotfiltrule_cp.ok",
+                "blast_pairs.idx.ok",
+                "cp_gene_blast_pairs.ok",
+                "cp_pfam_dat.ok",
+                "cp_ref_annot_cdna.ok",
+                "fusion_annot_lib.cp.ok",
+                "index_pfam_hits.ok",
+                "index_ref_annot_cdna.ok",
+                "makeblastdb.ok",
+                "mm2.splice_bed.ok",
+                "mm2_genome_idx.ok",
+                "ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "ref_annot.gtf.gene_spans.ok",
+                "ref_annot.gtf.mini.sortu.ok",
+                "ref_annot.gtf.ok",
+                "ref_genome.fa.ok",
+                "ref_genome_fai.ok",
+                "trans.blast.dat.cp.ok",
+                "trans.blast.dat.index.ok",
+                "validate_ctat_genome_lib.ok",
+                "blast_pairs.dat.gz",
+                "blast_pairs.idx",
+                "cancer_splicing_lib",
+                "cancer_splicing.idx",
+                "fusion_annot_lib.gz",
+                "fusion_annot_lib.idx",
+                "pfam_domains.dbm",
+                "refGene.bed",
+                "refGene.sort.bed.gz",
+                "refGene.sort.bed.gz.tbi",
+                "ref_annot.cdna.fa",
+                "ref_annot.cdna.fa.idx",
+                "ref_annot.cds",
+                "ref_annot.cdsplus.fa",
+                "ref_annot.cdsplus.fa.idx",
+                "ref_annot.gtf",
+                "ref_annot.gtf.gene_spans",
+                "ref_annot.gtf.mini.sortu",
+                "ref_annot.gtf.mm2.splice.bed",
+                "ref_annot.pep",
+                "ref_annot.prot_info.dbm",
+                "ref_genome.fa",
+                "ref_genome.fa.fai",
+                "ref_genome.fa.mm2",
+                "ref_genome.fa.ndb",
+                "ref_genome.fa.nhr",
+                "ref_genome.fa.nin",
+                "ref_genome.fa.njs",
+                "ref_genome.fa.not",
+                "ref_genome.fa.nsq",
+                "ref_genome.fa.ntf",
+                "ref_genome.fa.nto",
+                "ref_genome.fa.star.idx",
+                "Genome",
+                "Log.out",
+                "SA",
+                "SAindex",
+                "build.ok",
+                "chrLength.txt",
+                "chrName.txt",
+                "chrNameLength.txt",
+                "chrStart.txt",
+                "exonGeTrInfo.tab",
+                "exonInfo.tab",
+                "geneInfo.tab",
+                "genomeParameters.txt",
+                "sjdbInfo.txt",
+                "sjdbList.fromGTF.out.tab",
+                "sjdbList.out.tab",
+                "transcriptInfo.tab",
+                "trans.blast.align_coords.align_coords.dat",
+                "trans.blast.align_coords.align_coords.dbm",
+                "trans.blast.dat.gz"
+            ],
+            [
+                "AnnotFilterRule.pm:md5,f94966013cd0df9624a6dda0b75fefa0",
+                "fusion_annot_lib.gz:md5,23d82a5da81f91ca4e1ecd6481992a12",
+                "refGene.sort.bed.gz:md5,01bf4093e3e9dcad8ed3a0f45a381c95",
+                "ref_annot.gtf:md5,5ce8afe99ef3940a877a04caeacf9181",
+                "ref_annot.gtf.mini.sortu:md5,1d29ccecdbb7b40a99c84a02d6c2c1be",
+                "ref_annot.gtf.mm2.splice.bed:md5,340585ea1843bf06bf555575ddecf28c",
+                "ref_genome.fa:md5,ad699c56ed38566c7d3e9579486b1706",
+                "ref_genome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
+                "ref_genome.fa.mm2:md5,ce50979ea284748eb9f84ae88cfd930e",
+                "ref_genome.fa.ndb:md5,6ea574753b557610f62f6e4ab79e19f5",
+                "ref_genome.fa.nhr:md5,50f28dae71683c4394bfaf94a1ef4392",
+                "ref_genome.fa.not:md5,1e53e9d08f1d23af0299cfa87478a7bb",
+                "ref_genome.fa.nsq:md5,d2361e7871ce4cf51181c112a48f191b",
+                "ref_genome.fa.ntf:md5,de1250813f0c7affc6d12dac9d0fb6bb",
+                "ref_genome.fa.nto:md5,33cdeccccebe80329f1fdbee7f5874cb",
+                "Genome:md5,9e3efdd0901cabb5a2d589664a63b372",
+                "SA:md5,7dd9083264be9c6a2194d990bc10d237",
+                "SAindex:md5,ac4711df685109e04356db9e9cb9fb7f",
+                "chrLength.txt:md5,e02cd536b7281b894246863b160d5d06",
+                "chrName.txt:md5,f4d0d6595f423084e6b9472e40dfe6e8",
+                "chrNameLength.txt:md5,07a67d7ac441d7d30d80840b0927e717",
+                "chrStart.txt:md5,e2031239a74fe5ee9051e9364e4f608a",
+                "exonGeTrInfo.tab:md5,3c35618d07a8e35a0f9108699fcdda42",
+                "exonInfo.tab:md5,bcbb3f32fa31fe504cc737f337ad341c",
+                "geneInfo.tab:md5,db5db4b6e003904e9908fce7c05f0125",
+                "sjdbInfo.txt:md5,e4cc1bbf8bd687cfc3d7c2c702e6def7",
+                "sjdbList.fromGTF.out.tab:md5,8f3e8604b00d4067e4eb80aa476a8113",
+                "sjdbList.out.tab:md5,5d78dd49d5db24ca2c056b7ebe5c2059",
+                "transcriptInfo.tab:md5,b758c0ccaddcf0453bab5905b3cec4a2",
+                "trans.blast.align_coords.align_coords.dat:md5,9f6b7a75aea03a9671190be25ecdd4c2",
+                "trans.blast.dat.gz:md5,85ba5ea96c566f751ad83a3e4b8ab128"
+            ],
+            [
+                "versions.yml:md5,3554c611ce8fa5c21460b6a99e8e36dc"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-30T16:22:20.140859164"
+    },
+    "homo_sapiens - reference - stub": {
+        "content": [
+            [
+                "ctat_genome_lib_build_dir",
+                "AnnotFilterRule.pm",
+                "PFAM.domtblout.dat.gz",
+                "__chkpts",
+                "_fusion_annot_lib.idx.ok",
+                "_prot_info_db.ok",
+                "annotfiltrule_cp.ok",
+                "blast_pairs.idx.ok",
+                "cp_gene_blast_pairs.ok",
+                "cp_pfam_dat.ok",
+                "cp_ref_annot_cdna.ok",
+                "fusion_annot_lib.cp.ok",
+                "index_pfam_hits.ok",
+                "index_ref_annot_cdna.ok",
+                "makeblastdb.ok",
+                "mm2.splice_bed.ok",
+                "mm2_genome_idx.ok",
+                "ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "ref_annot.gtf.gene_spans.ok",
+                "ref_annot.gtf.mini.sortu.ok",
+                "ref_annot.gtf.ok",
+                "ref_genome.fa.ok",
+                "ref_genome_fai.ok",
+                "trans.blast.dat.cp.ok",
+                "trans.blast.dat.index.ok",
+                "validate_ctat_genome_lib.ok",
+                "blast_pairs.dat.gz",
+                "blast_pairs.idx",
+                "cancer_splicing_lib",
+                "cancer_splicing.idx",
+                "fusion_annot_lib.gz",
+                "fusion_annot_lib.idx",
+                "pfam_domains.dbm",
+                "refGene.bed",
+                "refGene.sort.bed.gz",
+                "refGene.sort.bed.gz.tbi",
+                "ref_annot.cdna.fa",
+                "ref_annot.cdna.fa.idx",
+                "ref_annot.cds",
+                "ref_annot.cdsplus.fa",
+                "ref_annot.cdsplus.fa.idx",
+                "ref_annot.gtf",
+                "ref_annot.gtf.gene_spans",
+                "ref_annot.gtf.mini.sortu",
+                "ref_annot.gtf.mm2.splice.bed",
+                "ref_annot.pep",
+                "ref_annot.prot_info.dbm",
+                "ref_genome.fa",
+                "ref_genome.fa.fai",
+                "ref_genome.fa.mm2",
+                "ref_genome.fa.ndb",
+                "ref_genome.fa.nhr",
+                "ref_genome.fa.nin",
+                "ref_genome.fa.njs",
+                "ref_genome.fa.not",
+                "ref_genome.fa.nsq",
+                "ref_genome.fa.ntf",
+                "ref_genome.fa.nto",
+                "ref_genome.fa.star.idx",
+                "Genome",
+                "Log.out",
+                "SA",
+                "SAindex",
+                "build.ok",
+                "chrLength.txt",
+                "chrName.txt",
+                "chrNameLength.txt",
+                "chrStart.txt",
+                "exonGeTrInfo.tab",
+                "exonInfo.tab",
+                "geneInfo.tab",
+                "genomeParameters.txt",
+                "sjdbInfo.txt",
+                "sjdbList.fromGTF.out.tab",
+                "sjdbList.out.tab",
+                "transcriptInfo.tab",
+                "trans.blast.align_coords.align_coords.dat",
+                "trans.blast.align_coords.align_coords.dbm",
+                "trans.blast.dat.gz"
+            ],
+            [
+                "AnnotFilterRule.pm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "PFAM.domtblout.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "_fusion_annot_lib.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "_prot_info_db.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "annotfiltrule_cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cp_gene_blast_pairs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cp_pfam_dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "cp_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusion_annot_lib.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "index_pfam_hits.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "index_ref_annot_cdna.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "makeblastdb.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mm2.splice_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "mm2_genome_idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.dfam_masked.fa.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.dfam_masked.fa.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.gene_spans.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.mini.sortu.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.cp.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "validate_ctat_genome_lib.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "blast_pairs.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "blast_pairs.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "fusion_annot_lib.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "fusion_annot_lib.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "pfam_domains.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "refGene.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "refGene.sort.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                "ref_annot.cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdna.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cds:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.cdsplus.fa.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.gene_spans:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.mini.sortu:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.gtf.mm2.splice.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.pep:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_annot.prot_info.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.mm2:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.ndb:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nhr:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nin:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.njs:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.not:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nsq:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.ntf:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "ref_genome.fa.nto:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Genome:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SA:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "SAindex:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "build.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrName.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrNameLength.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "chrStart.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "genomeParameters.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.align_coords.align_coords.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.align_coords.align_coords.dbm:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "trans.blast.dat.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+            ],
+            [
+                "versions.yml:md5,3554c611ce8fa5c21460b6a99e8e36dc"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
+        },
+        "timestamp": "2025-03-31T16:20:05.986978"
+    }
+}
diff --git a/modules/local/ctatsplicing/startocancerintrons/main.nf b/modules/nf-core/ctatsplicing/startocancerintrons/main.nf
similarity index 73%
rename from modules/local/ctatsplicing/startocancerintrons/main.nf
rename to modules/nf-core/ctatsplicing/startocancerintrons/main.nf
index 3d7e26027..dbaaf20c1 100644
--- a/modules/local/ctatsplicing/startocancerintrons/main.nf
+++ b/modules/nf-core/ctatsplicing/startocancerintrons/main.nf
@@ -2,29 +2,34 @@ process CTATSPLICING_STARTOCANCERINTRONS {
     tag "$meta.id"
     label 'process_single'
 
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://data.broadinstitute.org/Trinity/CTAT_SINGULARITY/CTAT-SPLICING/ctat_splicing.v0.0.2.simg' :
-        'docker.io/trinityctat/ctat_splicing:0.0.2' }"
+    container "nf-core/ctatsplicing:0.0.3"
 
     input:
     tuple val(meta), path(split_junction), path(junction), path(bam), path(bai)
     tuple val(meta2), path(genome_lib)
 
     output:
-    tuple val(meta), path("*.cancer_intron_reads.sorted.bam")       , emit: cancer_introns_sorted_bam
-    tuple val(meta), path("*.cancer_intron_reads.sorted.bam.bai")   , emit: cancer_introns_sorted_bai
-    tuple val(meta), path("*.gene_reads.sorted.sifted.bam")         , emit: gene_reads_sorted_bam
-    tuple val(meta), path("*.gene_reads.sorted.sifted.bam.bai")     , emit: gene_reads_sorted_bai
-    tuple val(meta), path("*.cancer.introns")                       , emit: cancer_introns
-    tuple val(meta), path("*.cancer.introns.prelim")                , emit: cancer_introns_prelim
-    tuple val(meta), path("*${prefix}.introns")                     , emit: introns
-    tuple val(meta), path("*.introns.for_IGV.bed")                  , emit: introns_igv_bed, optional: true
-    tuple val(meta), path("*.ctat-splicing.igv.html")               , emit: igv_html, optional: true
-    tuple val(meta), path("*.igv.tracks")                           , emit: igv_tracks, optional: true
-    tuple val(meta), path("*.chckpts")                              , emit: chckpts
-    path "versions.yml"                                             , emit: versions
+    tuple val(meta), path("*.cancer_intron_reads.sorted.bam")    , emit: cancer_introns_sorted_bam, optional: true
+    tuple val(meta), path("*.cancer_intron_reads.sorted.bam.bai"), emit: cancer_introns_sorted_bai, optional: true
+    tuple val(meta), path("*.gene_reads.sorted.sifted.bam")      , emit: gene_reads_sorted_bam    , optional: true
+    tuple val(meta), path("*.gene_reads.sorted.sifted.bam.bai")  , emit: gene_reads_sorted_bai    , optional: true
+    tuple val(meta), path("*.cancer.introns")                    , emit: cancer_introns
+    tuple val(meta), path("*.cancer.introns.prelim")             , emit: cancer_introns_prelim
+    tuple val(meta), path("*${prefix}.introns")                  , emit: introns
+    tuple val(meta), path("*.introns.for_IGV.bed")               , emit: introns_igv_bed          , optional: true
+    tuple val(meta), path("*.ctat-splicing.igv.html")            , emit: igv_html                 , optional: true
+    tuple val(meta), path("*.igv.tracks")                        , emit: igv_tracks               , optional: true
+    tuple val(meta), path("*.chckpts")                           , emit: chckpts                  , optional: true
+    path "versions.yml"                                          , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
 
     script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        error "CTATSPLICING_STARTOCANCERINTRONS module does not support Conda. Please use Docker / Singularity / Podman instead."
+    }
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
     def bam_arg = bam ? "--bam_file ${bam}" : ""
@@ -41,14 +46,6 @@ process CTATSPLICING_STARTOCANCERINTRONS {
         --ctat_genome_lib ${genome_lib} \\
         ${args}
 
-    # Create the missing outputs when no cancer introns are found
-    if [ \$? -eq 0 ]; then
-        touch ${prefix}.cancer_intron_reads.sorted.bam
-        touch ${prefix}.cancer_intron_reads.sorted.bam.bai
-        touch ${prefix}.gene_reads.sorted.sifted.bam
-        touch ${prefix}.gene_reads.sorted.sifted.bam.bai
-    fi
-
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
         ctat-splicing: $VERSION
diff --git a/modules/nf-core/ctatsplicing/startocancerintrons/meta.yml b/modules/nf-core/ctatsplicing/startocancerintrons/meta.yml
new file mode 100644
index 000000000..1f69d7988
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/startocancerintrons/meta.yml
@@ -0,0 +1,189 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "ctatsplicing_startocancerintrons"
+description: "Detection and annotation of aberrant splicing isoforms in cancer transcriptomes"
+long_description: |
+  This module detects and annotates aberrant splicing isoforms in cancer transcriptomes using CTAT-splicing.
+  It takes STAR alignment files, a sorted BAM file, and a CTAT genome library reference as input, and outputs various files related to cancer introns.
+  The output can be used for further analysis of splicing aberrations in cancer.
+  Make sure to provide the correct STAR alignment files and CTAT genome library reference.
+keywords:
+  - splicing
+  - cancer
+  - rna
+  - rnaseq
+tools:
+  - "ctatsplicing":
+      description: "Detection and annotation of cancer splicing aberrations"
+      homepage: "https://github.com/TrinityCTAT/CTAT-SPLICING"
+      documentation: "https://github.com/TrinityCTAT/CTAT-SPLICING"
+      tool_dev_url: "https://github.com/TrinityCTAT/CTAT-SPLICING"
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - split_junction:
+        type: file
+        description: "STAR alignment splice junction tab file"
+        pattern: "*.SJ.out.tab"
+    - junction:
+        type: file
+        description: "STAR alignment chimeric junction file"
+        pattern: "*.out.junction"
+    - bam:
+        type: file
+        description: "Sorted BAM/CRAM/SAM file"
+        pattern: "*.{bam,cram,sam}"
+        ontologies:
+          - edam: "http://edamontology.org/format_2572"
+          - edam: "http://edamontology.org/format_2573"
+          - edam: "http://edamontology.org/format_3462"
+    - bai:
+        type: file
+        description: "BAM/CRAM/SAM index file"
+        pattern: "*.{bai,crai,sai}"
+        ontologies:
+          - edam: "http://edamontology.org/format_3327"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. `[ id:'sample1', single_end:false ]`
+    - genome_lib:
+        type: file
+        description: "CTAT genome library reference with integrated cancer intron annotation"
+        pattern: "*"
+
+output:
+  cancer_introns_sorted_bam:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.cancer_intron_reads.sorted.bam":
+          type: file
+          description: "Sorted BAM file containing reads mapped to cancer introns"
+          pattern: "*.cancer_intron_reads.sorted.bam"
+          ontologies:
+            - edam: "http://edamontology.org/format_2572"
+  cancer_introns_sorted_bai:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.cancer_intron_reads.sorted.bam.bai":
+          type: file
+          description: "Index file for the sorted BAM of cancer introns"
+          pattern: "*.cancer_intron_reads.sorted.bam.bai"
+          ontologies:
+            - edam: "http://edamontology.org/format_3327"
+  gene_reads_sorted_bam:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gene_reads.sorted.sifted.bam":
+          type: file
+          description: "Sorted BAM file containing reads mapped to gene regions after downsampling coverage"
+          pattern: "*.gene_reads.sorted.sifted.bam"
+          ontologies:
+            - edam: "http://edamontology.org/format_2572"
+  gene_reads_sorted_bai:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.gene_reads.sorted.sifted.bam.bai":
+          type: file
+          description: "Index file for the sorted BAM of gene reads after filtering"
+          pattern: "*.gene_reads.sorted.sifted.bam.bai"
+          ontologies:
+            - edam: "http://edamontology.org/format_3327"
+  cancer_introns:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.cancer.introns":
+          type: file
+          description: "File containing detected and filtered introns that are found to be enriched in cancer transcriptomes"
+          pattern: "*.cancer.introns"
+  cancer_introns_prelim:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.cancer.introns.prelim":
+          type: file
+          description: "File containing detected introns that have at least one supporting read and are found to be enriched in cancer transcriptomes"
+          pattern: "*.cancer.introns.prelim"
+  introns:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*${prefix}.introns":
+          type: file
+          description: "File containing all detected introns"
+          pattern: "*${prefix}.introns"
+  introns_igv_bed:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.introns.for_IGV.bed":
+          type: file
+          description: "Bed file used as input for IGV-report visualization, containing cancer introns"
+          pattern: "*.introns.for_IGV.bed"
+          ontologies:
+            - edam: "http://edamontology.org/format_3586"
+  igv_html:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.ctat-splicing.igv.html":
+          type: file
+          description: "Self-contained IGV-report in HTML format for visualization of cancer introns"
+          pattern: "*.ctat-splicing.igv.html"
+  igv_tracks:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.igv.tracks":
+          type: file
+          description: "IGV tracks file for visualizing cancer introns in IGV"
+          pattern: "*.igv.tracks"
+  chckpts:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.chckpts":
+          type: file
+          description: "Checkpoint files for CTAT-SPLICING, useful for debugging or resuming interrupted runs"
+          pattern: "*.chckpts"
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+authors:
+  - "@anoronh4"
+  - "@nvnieuwk"
+maintainers:
+  - "@anoronh4"
diff --git a/modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test b/modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test
new file mode 100644
index 000000000..102423258
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test
@@ -0,0 +1,186 @@
+nextflow_process {
+
+    name "Test Process CTATSPLICING_STARTOCANCERINTRONS"
+    script "../main.nf"
+    process "CTATSPLICING_STARTOCANCERINTRONS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "ctatsplicing"
+    tag "ctatsplicing/startocancerintrons"
+    tag "ctatsplicing/prepgenomelib"
+    tag "star/align"
+    tag "samtools/index"
+    tag "starfusion/build"
+
+    test("homo_sapiens - bam - junctions") {
+
+        config './nextflow.config'
+        setup {
+            run("STARFUSION_BUILD") {
+                script "../../../starfusion/build/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'minigenome_fasta' ],
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
+                    ]
+                    input[1] = [
+                        [ id:'minigenome_gtf' ],
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
+                    ]
+                    input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                    input[3] = "homo_sapiens"
+                    input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                    input[5] = [
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
+                        "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                    ]
+                    input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                    """
+                }
+            }
+            run("CTATSPLICING_PREPGENOMELIB") {
+                script "../../prepgenomelib/main.nf"
+                process {
+                    """
+                    input[0] = STARFUSION_BUILD.out.reference
+                    input[1] = [
+                        file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
+                    ]
+                    """
+                }
+            }
+            run("STAR_ALIGN") {
+                script "../../../star/align/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test', single_end:true ], // meta map
+                        [
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                        ]
+                    ])
+                    input[1] = CTATSPLICING_PREPGENOMELIB.out.reference.map{ meta, reference ->
+                        [ meta, "\$reference/ref_genome.fa.star.idx" ]
+                    }
+                    input[2] = CTATSPLICING_PREPGENOMELIB.out.reference.map{ meta, reference ->
+                        [ meta, "\$reference/ref_annot.gtf" ]
+                    }
+                    input[3] = false
+                    input[4] = 'illumina'
+                    input[5] = false
+                    """
+                }
+            }
+            run("SAMTOOLS_INDEX") {
+                script "../../../samtools/index/main.nf"
+                process {
+                    """
+                    input[0] = STAR_ALIGN.out.bam_sorted_aligned
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = STAR_ALIGN.out.spl_junc_tab
+                    .join(STAR_ALIGN.out.junction, by:[0])
+                    .join(STAR_ALIGN.out.bam, by:[0])
+                    .join(SAMTOOLS_INDEX.out.bai, by:[0])
+                input[1] = CTATSPLICING_PREPGENOMELIB.out.reference
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(
+                    process.out.cancer_introns,
+                    process.out.cancer_introns_prelim,
+                    file(process.out.introns[0][1]).name,
+                    process.out.versions
+                ).match() }
+            )
+        }
+
+    }
+
+    test("homo_sapiens - bam - junctions - stub") {
+
+        options "-stub"
+        config './nextflow.config'
+        setup {
+            run("CTATSPLICING_PREPGENOMELIB") {
+                script "../../prepgenomelib/main.nf"
+                process {
+                    """
+                    new File('ctat_genome_lib_build_dir').mkdirs()
+                    input[0] = [[id:"ctat_genome_lib_build_dir"],file("ctat_genome_lib_build_dir/")]
+                    input[1] = [
+                        file("https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz", checkIfExists:true)
+                    ]
+                    """
+                }
+            }
+                run("STAR_ALIGN") {
+                script "../../../star/align/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test', single_end:true ], // meta map
+                        [
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                        ]
+                    ])
+                    input[1] = CTATSPLICING_PREPGENOMELIB.out.reference.map{ meta, reference ->
+                        [ meta, "\$reference/ref_genome.fa.star.idx" ]
+                    }
+                    input[2] = CTATSPLICING_PREPGENOMELIB.out.reference.map{ meta, reference ->
+                        [ meta, "\$reference/ref_annot.gtf" ]
+                    }
+                    input[3] = false
+                    input[4] = 'illumina'
+                    input[5] = false
+                    """
+                }
+            }
+            run("SAMTOOLS_INDEX") {
+                script "../../../samtools/index/main.nf"
+                process {
+                    """
+                    input[0] = STAR_ALIGN.out.bam_sorted_aligned
+                    """
+                }
+
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = STAR_ALIGN.out.spl_junc_tab
+                    .join(STAR_ALIGN.out.junction, by:[0])
+                    .join(STAR_ALIGN.out.bam, by:[0])
+                    .join(SAMTOOLS_INDEX.out.bai, by:[0])
+                input[1] = CTATSPLICING_PREPGENOMELIB.out.reference
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test.snap b/modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test.snap
similarity index 55%
rename from modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test.snap
rename to modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test.snap
index b0ee34166..604821ef0 100644
--- a/modules/local/ctatsplicing/startocancerintrons/tests/main.nf.test.snap
+++ b/modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test.snap
@@ -1,98 +1,145 @@
 {
-    "test without BAM": {
+    "homo_sapiens - bam - junctions": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.cancer.introns:md5,c0eb94972d845218c2e470b85c55871b"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.cancer.introns.prelim:md5,c0eb94972d845218c2e470b85c55871b"
+                ]
+            ],
+            "test.introns",
+            [
+                "versions.yml:md5,fcf861a15f9951342a874b6bc476a37e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-31T03:52:51.492834709"
+    },
+    "homo_sapiens - bam - junctions - stub": {
         "content": [
             {
-                "cancer_introns": [
+                "0": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.cancer.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.cancer_intron_reads.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "cancer_introns_prelim": [
+                "1": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.cancer.introns.prelim:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.cancer_intron_reads.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "cancer_introns_sorted_bai": [
+                "10": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.cancer_intron_reads.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.chckpts:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "cancer_introns_sorted_bam": [
+                "11": [
+                    "versions.yml:md5,fcf861a15f9951342a874b6bc476a37e"
+                ],
+                "2": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.cancer_intron_reads.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.gene_reads.sorted.sifted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "chckpts": [
+                "3": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.chckpts:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.gene_reads.sorted.sifted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "gene_reads_sorted_bai": [
+                "4": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.gene_reads.sorted.sifted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.cancer.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "gene_reads_sorted_bam": [
+                "5": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.gene_reads.sorted.sifted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.cancer.introns.prelim:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "igv_html": [
-                    
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
-                "igv_tracks": [
-                    
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.introns.for_IGV.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
-                "introns": [
+                "8": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
-                        "test.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.ctat-splicing.igv.html:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
                 ],
-                "introns_igv_bed": [
-                    
+                "9": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.igv.tracks:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
                 ],
-                "versions": [
-                    "versions.yml:md5,fcf861a15f9951342a874b6bc476a37e"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.1",
-            "nextflow": "24.10.1"
-        },
-        "timestamp": "2024-12-17T13:35:13.723215847"
-    },
-    "test with BAM": {
-        "content": [
-            {
                 "cancer_introns": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.cancer.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -100,7 +147,8 @@
                 "cancer_introns_prelim": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.cancer.introns.prelim:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -108,7 +156,8 @@
                 "cancer_introns_sorted_bai": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.cancer_intron_reads.sorted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -116,7 +165,8 @@
                 "cancer_introns_sorted_bam": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.cancer_intron_reads.sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -124,7 +174,8 @@
                 "chckpts": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.chckpts:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -132,7 +183,8 @@
                 "gene_reads_sorted_bai": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.gene_reads.sorted.sifted.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -140,7 +192,8 @@
                 "gene_reads_sorted_bam": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.gene_reads.sorted.sifted.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -148,7 +201,8 @@
                 "igv_html": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.ctat-splicing.igv.html:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -156,7 +210,8 @@
                 "igv_tracks": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.igv.tracks:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -164,7 +219,8 @@
                 "introns": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.introns:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -172,7 +228,8 @@
                 "introns_igv_bed": [
                     [
                         {
-                            "id": "test"
+                            "id": "test",
+                            "single_end": true
                         },
                         "test.introns.for_IGV.bed:md5,d41d8cd98f00b204e9800998ecf8427e"
                     ]
@@ -183,9 +240,9 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.1",
-            "nextflow": "24.10.1"
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.3"
         },
-        "timestamp": "2024-12-17T13:33:27.36677449"
+        "timestamp": "2025-04-03T15:19:53.688973"
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/ctatsplicing/startocancerintrons/tests/nextflow.config b/modules/nf-core/ctatsplicing/startocancerintrons/tests/nextflow.config
new file mode 100644
index 000000000..817872ce3
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/startocancerintrons/tests/nextflow.config
@@ -0,0 +1,33 @@
+process {
+    withName: STAR_ALIGN {
+        ext.args = {
+            [
+                "--readFilesCommand zcat",
+                "--outSAMtype BAM SortedByCoordinate",
+                "--outReadsUnmapped None",
+                "--twopassMode None",
+                "--outSAMstrandField intronMotif",
+                "--outSAMunmapped Within",
+                "--chimSegmentMin 12",
+                "--chimJunctionOverhangMin 8",
+                "--chimOutJunctionFormat 1",
+                "--alignSJDBoverhangMin 10",
+                "--alignMatesGapMax 100000",
+                "--alignIntronMax 100000",
+                "--alignSJstitchMismatchNmax 5 -1 5 5",
+                "--chimMultimapScoreRange 3",
+                "--chimScoreJunctionNonGTAG -4",
+                "--chimMultimapNmax 20",
+                "--chimNonchimScoreDropMin 10",
+                "--peOverlapNbasesMin 12",
+                "--peOverlapMMp 0.1",
+                "--alignInsertionFlush Right",
+                "--alignSplicedMateMapLminOverLmate 0",
+                "--alignSplicedMateMapLmin 30"
+            ].join(" ").trim()
+	    }
+    }
+    withName: CTATSPLICING_STARTOCANCERINTRONS {
+        ext.args = "--min_total_reads 1 --vis"
+    }
+}

From f4cf3efb071a92c7da70b10c71dd91a799a00a6e Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 12:22:21 +0000
Subject: [PATCH 758/887] chore: replace local fusioninspector for nf-core

---
 modules/local/fusioninspector/main.nf         | 184 --------
 modules/local/fusioninspector/meta.yml        |  40 --
 .../fusioninspector/tests/main.nf.test.snap   | 414 ------------------
 .../fusioninspector/tests/nextflow.config     |   4 -
 .../fusioninspector/environment.yml           |   2 +-
 modules/nf-core/fusioninspector/main.nf       |  66 +++
 modules/nf-core/fusioninspector/meta.yml      | 150 +++++++
 .../fusioninspector/tests/main.nf.test        |  37 +-
 .../fusioninspector/tests/main.nf.test.snap   | 199 +++++++++
 .../fusioninspector/tests/nextflow.config     |   5 +
 10 files changed, 442 insertions(+), 659 deletions(-)
 delete mode 100644 modules/local/fusioninspector/main.nf
 delete mode 100644 modules/local/fusioninspector/meta.yml
 delete mode 100644 modules/local/fusioninspector/tests/main.nf.test.snap
 delete mode 100644 modules/local/fusioninspector/tests/nextflow.config
 rename modules/{local => nf-core}/fusioninspector/environment.yml (89%)
 create mode 100644 modules/nf-core/fusioninspector/main.nf
 create mode 100644 modules/nf-core/fusioninspector/meta.yml
 rename modules/{local => nf-core}/fusioninspector/tests/main.nf.test (57%)
 create mode 100644 modules/nf-core/fusioninspector/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/fusioninspector/tests/nextflow.config

diff --git a/modules/local/fusioninspector/main.nf b/modules/local/fusioninspector/main.nf
deleted file mode 100644
index 54a546983..000000000
--- a/modules/local/fusioninspector/main.nf
+++ /dev/null
@@ -1,184 +0,0 @@
-process FUSIONINSPECTOR {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/13/139b94a1f10c6e23a8c27eaed1e5a689db978a513d0ee155e74d35f0970814fe/data' :
-        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:c6147971d107ab11'}"
-
-    input:
-    tuple val(meta), path(reads), path(fusion_list)
-    tuple val(meta2), path(reference)
-
-    output:
-    tuple val(meta), path("*FusionInspector.fusions.tsv")                  , emit: tsv
-    tuple val(meta), path("fi_workdir/*.gtf")                              , optional:true, emit: out_gtf
-    tuple val(meta), path("*FusionInspector.log")                          , emit: log
-    tuple val(meta), path("*html")                                         , emit: html
-    tuple val(meta), path("*abridged.tsv")                                 , emit: abridged_tsv
-    tuple val(meta), path("IGV_inputs")                                    , emit: igv_inputs
-    tuple val(meta), path("fi_workdir")                                    , emit: fi_workdir
-    tuple val(meta), path("chckpts_dir")                                   , emit: chckpts_dir
-    path "versions.yml"                                                    , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def fasta = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    """
-    FusionInspector \\
-        --fusions $fusion_list \\
-        --genome_lib ${reference} \\
-        $fasta \\
-        --CPU ${task.cpus} \\
-        -O . \\
-        --out_prefix $prefix \\
-        --vis $args $args2
-
-    # Touch the output files to make sure they exist
-    touch FusionInspector.log
-    touch ${prefix}.FusionInspector.fusions.abridged.tsv
-    touch ${prefix}.FusionInspector.fusions.tsv
-    touch ${prefix}.fusion_inspector_web.html
-    mkdir -p IGV_inputs
-    mkdir -p fi_workdir
-    mkdir -p chckpts_dir
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch FusionInspector.log
-    touch ${prefix}.FusionInspector.fusions.abridged.tsv
-    touch ${prefix}.FusionInspector.fusions.tsv
-    touch ${prefix}.fusion_inspector_web.html
-    mkdir -p chckpts_dir
-    touch chckpts_dir/add_FFPM.ok
-    touch chckpts_dir/add_splice_info.ok
-    touch chckpts_dir/append_microH_info.ok
-    touch chckpts_dir/blast_filter.ok
-    touch chckpts_dir/coalesce_junc_n_span.ok
-    touch chckpts_dir/cp_consol_bam.ok
-    touch chckpts_dir/cp_contigs_file_workdir
-    touch chckpts_dir/cp_final.ok
-    touch chckpts_dir/cp_gtf_file_workdir.ok
-    touch chckpts_dir/cp_tracks_json.ok
-    touch chckpts_dir/create_fi_igvjs.ok
-    touch chckpts_dir/cytoband.ok
-    touch chckpts_dir/EM_adj_counts.ok
-    touch chckpts_dir/filter_by_frag_threshs.ok
-    touch chckpts_dir/final.abridged.ok
-    touch chckpts_dir/fusion_annotator.ok
-    touch chckpts_dir/fusion_coding_region_effect.ok
-    touch chckpts_dir/fusion_contigs.ok
-    touch chckpts_dir/fusion_reports_html.ok
-    touch chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok
-    touch chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok
-    touch chckpts_dir/index_consol_bam.ok
-    touch chckpts_dir/init_EM_adj_counts.ok
-    touch chckpts_dir/init_spanning_reads_bam.ok
-    touch chckpts_dir/mark_dup_reads.ok
-    touch chckpts_dir/mark_dups_reads.index.ok
-    touch chckpts_dir/merged_contig_fai.ok
-    touch chckpts_dir/merged_contig_gtf_to_bed.ok
-    touch chckpts_dir/microH.dat.ok
-    touch chckpts_dir/prep_igv_extract_junc_reads.ok
-    touch chckpts_dir/prep_igv_junc_reads_bam.ok
-    touch chckpts_dir/prep_igv_pfam_bed.ok
-    touch chckpts_dir/prep_igv_pfam_gff3.ok
-    touch chckpts_dir/prep_igv_seqsim_bed.ok
-    touch chckpts_dir/prep_igv_seqsim_gff3.ok
-    touch chckpts_dir/prep_spanning_reads.ok
-    touch chckpts_dir/run_STAR.ok
-    touch chckpts_dir/samtools_idx_junc_reads_bam.ok
-    touch chckpts_dir/samtools_index_span_reads_bam.ok
-    touch chckpts_dir/span_reads_acc.ok
-    touch chckpts_dir/${prefix}.bed.bedsort.ok
-    touch chckpts_dir/${prefix}.bed.bgzip.ok
-    touch chckpts_dir/${prefix}.bed.tabix.ok
-    mkdir -p fi_workdir/_STARgenome
-    touch fi_workdir/Log.final.out
-    touch fi_workdir/Log.out
-    touch fi_workdir/Log.progress.out
-    touch fi_workdir/microH.dat
-    touch fi_workdir/pipeliner.456.cmds
-    touch fi_workdir/SJ.out.tab
-    touch fi_workdir/star_align.ok
-    touch fi_workdir/_STARgenome/exonGeTrInfo.tab
-    touch fi_workdir/_STARgenome/exonInfo.tab
-    touch fi_workdir/_STARgenome/geneInfo.tab
-    touch fi_workdir/_STARgenome/sjdbInfo.txt
-    touch fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab
-    touch fi_workdir/_STARgenome/sjdbList.out.tab
-    touch fi_workdir/_STARgenome/transcriptInfo.tab
-    touch fi_workdir/${prefix}.fa
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.fusion_junction_read_accs
-    touch fi_workdir/${prefix}.fusion_preds.coalesced.summary.fusion_spanning_read_accs
-    touch fi_workdir/${prefix}.gtf
-    touch fi_workdir/${prefix}.igv.Pfam.gff3
-    touch fi_workdir/${prefix}.igv.seqsimilar.gff3
-    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter
-    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj
-    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM
-    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH
-    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated
-    touch fi_workdir/${prefix}.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.bai
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_junction_info
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_spanning_info
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.read_align_counts.idx
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok
-    touch fi_workdir/${prefix}.star.cSorted.dupsMarked.bam.spanning_reads_want.idx
-    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam
-    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam.bai
-    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam.bai.ok
-    touch fi_workdir/${prefix}.star.sortedByCoord.out.bam.ok
-    mkdir -p IGV_inputs
-    touch IGV_inputs/cytoBand.txt
-    touch IGV_inputs/${prefix}.bed
-    gzip -c < /dev/null > IGV_inputs/${prefix}.bed.sorted.bed.gz
-    touch IGV_inputs/${prefix}.bed.sorted.bed.gz.tbi
-    touch IGV_inputs/${prefix}.consolidated.bam
-    touch IGV_inputs/${prefix}.consolidated.bam.bai
-    touch IGV_inputs/${prefix}.fa
-    touch IGV_inputs/${prefix}.fa.fai
-    touch IGV_inputs/${prefix}.fusion_inspector_web.json
-    touch IGV_inputs/${prefix}.gtf
-    touch IGV_inputs/${prefix}.igv.Pfam.bed
-    touch IGV_inputs/${prefix}.igv.seqsimilar.bed
-    touch IGV_inputs/${prefix}.junction_reads.bam
-    touch IGV_inputs/${prefix}.junction_reads.bam.bai
-    touch IGV_inputs/${prefix}.ROI.bed
-    touch IGV_inputs/${prefix}.spanning_reads.bam
-    touch IGV_inputs/${prefix}.spanning_reads.bam.bai
-    touch IGV_inputs/tracks.json
-    touch IGV_inputs/TrinityFusion.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/fusioninspector/meta.yml b/modules/local/fusioninspector/meta.yml
deleted file mode 100644
index cc03239be..000000000
--- a/modules/local/fusioninspector/meta.yml
+++ /dev/null
@@ -1,40 +0,0 @@
-name: fusioninspector
-description: Validation of Fusion Transcript Predictions
-keywords:
-  - fusioninspector
-tools:
-  - fusioninspector:
-      description: Validation of Fusion Transcript Predictions
-      homepage: https://github.com/FusionInspector/FusionInspector
-      documentation: https://github.com/FusionInspector/FusionInspector/wiki
-      tool_dev_url: https://github.com/FusionInspector/FusionInspector
-      doi: 10.1101/2021.08.02.454639"
-      licence: https://github.com/FusionInspector/FusionInspector/blob/master/LICENSE.txt
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - reads:
-      type: file
-      description: FASTQ file
-      pattern: "*.{fastq*}"
-  - reference:
-      type: directory
-      description: Path to ctat references
-      pattern: "*"
-
-output:
-  - versions:
-      type: file
-      description: File containing software versions
-      pattern: "versions.yml"
-  - reference:
-      type: directory
-      description: Genome resource path
-      pattern: "*"
-
-authors:
-  - "@rannick"
diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
deleted file mode 100644
index 79e97ea6e..000000000
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ /dev/null
@@ -1,414 +0,0 @@
-{
-    "FUSIONINSPECTOR stub": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.fusion_inspector_web.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "4": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "5": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cytoBand.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.ROI.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.sorted.bed.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.sorted.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.consolidated.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.consolidated.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_inspector_web.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.junction_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.junction_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.spanning_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.spanning_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "tracks.json:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "6": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            "Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            [
-                                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
-                            ],
-                            "microH.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "pipeliner.456.cmds:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "star_align.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.fusion_junction_read_accs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.fusion_spanning_read_accs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.Pfam.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.seqsimilar.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_junction_info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_spanning_info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.read_align_counts.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam.bai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "7": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            "EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "add_FFPM.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "add_splice_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "append_microH_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "blast_filter.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "coalesce_junc_n_span.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_contigs_file_workdir:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_final.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_gtf_file_workdir.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_tracks_json.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "create_fi_igvjs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cytoband.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "filter_by_frag_threshs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "final.abridged.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_annotator.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_coding_region_effect.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_contigs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_reports_html.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "get_fusion_JUNCTION_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "get_fusion_SPANNING_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "index_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "init_EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "init_spanning_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "mark_dup_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "mark_dups_reads.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "merged_contig_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "merged_contig_gtf_to_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "microH.dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_extract_junc_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_pfam_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_pfam_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_seqsim_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_seqsim_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_spanning_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "run_STAR.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "samtools_idx_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "samtools_index_span_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "span_reads_acc.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.bedsort.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.bgzip.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.tabix.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "8": [
-                    "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
-                ],
-                "abridged_tsv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "chckpts_dir": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            "EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "add_FFPM.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "add_splice_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "append_microH_info.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "blast_filter.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "coalesce_junc_n_span.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_contigs_file_workdir:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_final.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_gtf_file_workdir.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cp_tracks_json.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "create_fi_igvjs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cytoband.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "filter_by_frag_threshs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "final.abridged.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_annotator.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_coding_region_effect.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_contigs.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "fusion_reports_html.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "get_fusion_JUNCTION_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "get_fusion_SPANNING_reads_from_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "index_consol_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "init_EM_adj_counts.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "init_spanning_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "mark_dup_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "mark_dups_reads.index.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "merged_contig_fai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "merged_contig_gtf_to_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "microH.dat.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_extract_junc_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_pfam_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_pfam_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_seqsim_bed.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_igv_seqsim_gff3.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "prep_spanning_reads.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "run_STAR.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "samtools_idx_junc_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "samtools_index_span_reads_bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "span_reads_acc.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.bedsort.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.bgzip.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.tabix.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "fi_workdir": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            "Log.final.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "Log.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "Log.progress.out:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "SJ.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            [
-                                "exonGeTrInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "exonInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "geneInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbInfo.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbList.fromGTF.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "sjdbList.out.tab:md5,d41d8cd98f00b204e9800998ecf8427e",
-                                "transcriptInfo.tab:md5,d41d8cd98f00b204e9800998ecf8427e"
-                            ],
-                            "microH.dat:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "pipeliner.456.cmds:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "star_align.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.fusion_junction_read_accs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_preds.coalesced.summary.fusion_spanning_read_accs:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.Pfam.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.seqsimilar.gff3:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_junction_info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.fusion_spanning_info:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.read_align_counts.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam.bai.ok:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.star.sortedByCoord.out.bam.ok:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "html": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.fusion_inspector_web.html:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "igv_inputs": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        [
-                            "TrinityFusion.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "cytoBand.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.ROI.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.sorted.bed.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.bed.sorted.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.consolidated.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.consolidated.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fa:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fa.fai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.fusion_inspector_web.json:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.Pfam.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.igv.seqsimilar.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.junction_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.junction_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.spanning_reads.bam:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "test.spanning_reads.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e",
-                            "tracks.json:md5,d41d8cd98f00b204e9800998ecf8427e"
-                        ]
-                    ]
-                ],
-                "log": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "out_gtf": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "tsv": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,ad8d0da5929dfa70794bccf8765ddb23"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-30T15:11:46.208438"
-    },
-    "FUSIONINSPECTOR": {
-        "content": [
-            true,
-            true,
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.gtf:md5,d523061bd8b443a014d4cca9406ec772"
-                ]
-            ],
-            true
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-28T14:58:15.527794"
-    }
-}
\ No newline at end of file
diff --git a/modules/local/fusioninspector/tests/nextflow.config b/modules/local/fusioninspector/tests/nextflow.config
deleted file mode 100644
index 1cc66c43e..000000000
--- a/modules/local/fusioninspector/tests/nextflow.config
+++ /dev/null
@@ -1,4 +0,0 @@
-process {
-    withName: 'FUSIONINSPECTOR' {
-        ext.args = '--annotate --examine_coding_effect'    }
-}
diff --git a/modules/local/fusioninspector/environment.yml b/modules/nf-core/fusioninspector/environment.yml
similarity index 89%
rename from modules/local/fusioninspector/environment.yml
rename to modules/nf-core/fusioninspector/environment.yml
index 26605058b..e0e8ab3f3 100644
--- a/modules/local/fusioninspector/environment.yml
+++ b/modules/nf-core/fusioninspector/environment.yml
@@ -7,6 +7,6 @@ dependencies:
   - bioconda::perl-json-xs=4.03
   - bioconda::pysam=0.22.1
   - conda-forge::perl-carp-assert=0.21
-  - pip
+  - conda-forge::pip==24.3.1
   - pip:
       - intervaltree==3.1.0
diff --git a/modules/nf-core/fusioninspector/main.nf b/modules/nf-core/fusioninspector/main.nf
new file mode 100644
index 000000000..5e2546692
--- /dev/null
+++ b/modules/nf-core/fusioninspector/main.nf
@@ -0,0 +1,66 @@
+process FUSIONINSPECTOR {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/13/139b94a1f10c6e23a8c27eaed1e5a689db978a513d0ee155e74d35f0970814fe/data' :
+        'community.wave.seqera.io/library/fusion-inspector_igv-reports_perl-json-xs_pysam_pruned:c6147971d107ab11'}"
+
+    input:
+    tuple val(meta), path(reads), path(fusion_list)
+    tuple val(meta2), path(reference)
+
+    output:
+    tuple val(meta), path("*FusionInspector.fusions.tsv"), emit: tsv         , optional:true
+    tuple val(meta), path("fi_workdir/*.gtf")            , emit: out_gtf     , optional:true
+    tuple val(meta), path("*FusionInspector.log")        , emit: log         , optional:true
+    tuple val(meta), path("*html")                       , emit: html        , optional:true
+    tuple val(meta), path("*abridged.tsv")               , emit: abridged_tsv, optional:true
+    tuple val(meta), path("IGV_inputs")                  , emit: igv_inputs  , optional:true
+    tuple val(meta), path("fi_workdir")                  , emit: fi_workdir  , optional:true
+    tuple val(meta), path("chckpts_dir")                 , emit: chckpts_dir , optional:true
+    path  "versions.yml"                                 , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def fasta  = meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}"
+    def args   = task.ext.args   ?: ''
+    def args2  = task.ext.args2  ?: ''
+    """
+    FusionInspector \\
+        --fusions $fusion_list \\
+        --genome_lib ${reference} \\
+        $fasta \\
+        --CPU ${task.cpus} \\
+        -O . \\
+        --out_prefix $prefix \\
+        --vis $args $args2
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        FusionInspector: \$(FusionInspector --version 2>&1 | grep -i 'version' | sed -e 's/FusionInspector version: //' -e 's/[[:space:]]//g')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch FusionInspector.log
+    touch ${prefix}.FusionInspector.fusions.abridged.tsv
+    touch ${prefix}.FusionInspector.fusions.tsv
+    touch ${prefix}.fusion_inspector_web.html
+    mkdir -p chckpts_dir
+    mkdir -p fi_workdir
+    touch fi_workdir/${prefix}.gtf
+    mkdir -p IGV_inputs
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        FusionInspector: \$(FusionInspector --version 2>&1 | grep -i 'version' | sed -e 's/FusionInspector version: //' -e 's/[[:space:]]//g')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/fusioninspector/meta.yml b/modules/nf-core/fusioninspector/meta.yml
new file mode 100644
index 000000000..1e94eee2d
--- /dev/null
+++ b/modules/nf-core/fusioninspector/meta.yml
@@ -0,0 +1,150 @@
+name: fusioninspector
+description: Validation of Fusion Transcript Predictions
+keywords:
+  - fusioninspector
+  - fusion
+  - RNA-seq
+  - fastq
+tools:
+  - fusioninspector:
+      description: Validation of Fusion Transcript Predictions
+      homepage: https://github.com/FusionInspector/FusionInspector
+      documentation: https://github.com/FusionInspector/FusionInspector/wiki
+      tool_dev_url: https://github.com/FusionInspector/FusionInspector
+      doi: 10.1101/2021.08.02.454639"
+      licence: ["BSD-3-Clause"]
+      identifier: ""
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: FASTQ file
+        pattern: "*.{fastq*}"
+        ontologies:
+          - edam: "http://edamontology.org/format_1930" # FASTQ
+    - fusion_list:
+        type: file
+        description: Fusion targets list
+        pattern: "*.{txt}"
+        ontologies:
+          - edam: "http://edamontology.org/format_2330" # Textual format
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reference:
+        type: directory
+        description: Path to CTAT references
+        pattern: "*"
+
+output:
+  tsv:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*FusionInspector.fusions.tsv":
+          type: file
+          description: FusionInspector output TSV file
+          pattern: "*.tsv"
+          ontologies:
+            - edam: "http://edamontology.org/format_3475" # TSV
+  out_gtf:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - fi_workdir/*.gtf:
+          type: file
+          description: GTF output file
+          pattern: "*.gtf"
+          ontologies:
+            - edam: "http://edamontology.org/format_2306" # GTF
+  log:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*FusionInspector.log":
+          type: file
+          description: FusionInspector log file
+          pattern: "*.log"
+          ontologies:
+            - edam: "http://edamontology.org/data_1678" # Log file
+            - edam: "http://edamontology.org/format_2330" # Plain text
+  html:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*html":
+          type: file
+          description: HTML output files
+          pattern: "*.html"
+          ontologies:
+            - edam: "http://edamontology.org/format_2331" # HTML
+  abridged_tsv:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*abridged.tsv":
+          type: file
+          description: Abridged TSV output file
+          pattern: "*.tsv"
+          ontologies:
+            - edam: "http://edamontology.org/format_3475" # TSV
+  igv_inputs:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - IGV_inputs:
+          type: directory
+          description: IGV inputs directory
+          pattern: "IGV_inputs"
+  fi_workdir:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - fi_workdir:
+          type: directory
+          description: FusionInspector work directory
+          pattern: "fi_workdir"
+  chckpts_dir:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - chckpts_dir:
+          type: directory
+          description: Checkpoints directory
+          pattern: "chckpts_dir"
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@rannick"
+  - "@delfiterradas"
+  - "@sofiromano"
+  - "@alanmmobbs93"
+  - "@martings"
diff --git a/modules/local/fusioninspector/tests/main.nf.test b/modules/nf-core/fusioninspector/tests/main.nf.test
similarity index 57%
rename from modules/local/fusioninspector/tests/main.nf.test
rename to modules/nf-core/fusioninspector/tests/main.nf.test
index fb1374633..01e186f7f 100644
--- a/modules/local/fusioninspector/tests/main.nf.test
+++ b/modules/nf-core/fusioninspector/tests/main.nf.test
@@ -6,13 +6,15 @@ nextflow_process {
     tag "modules"
     tag "modules_local"
     tag "fusioninspector"
+    tag "starfusion/build"
+    tag "modules_nfcore"
 
-    test("FUSIONINSPECTOR") {
+    test("FUSIONINSPECTOR - test") {
         config './nextflow.config'
 
         setup {
             run("STARFUSION_BUILD") {
-                script "../../../nf-core/starfusion/build/main.nf"
+                script "../../starfusion/build/main.nf"
                 process {
                     """
                     input[0] = [
@@ -26,13 +28,13 @@ nextflow_process {
 
                     input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                     input[3] = "homo_sapiens"
-                    input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
+                    input[4] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/Pfam-A.hmm.gz')
                     input[5] = [
-                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
-                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
-                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
-                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
-                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3f'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3i'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3m'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3p')
                     ]
                     input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                     """
@@ -46,8 +48,8 @@ nextflow_process {
                 input[0] = [
                     [ id:'test', single_end:false ], // meta map
                     [
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_1.fastq.gz'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_2.fastq.gz')
                     ], // reads
                     [
                         file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
@@ -61,18 +63,21 @@ nextflow_process {
         then {
             assertAll(
                 { assert process.success },
+                { assert file(process.out.tsv[0][1]).getText().contains("THRA--AC090627.1\t72\t83\t72.00\t80.15") },
+                { assert file(process.out.abridged_tsv[0][1]).getText().contains("THRA--AC090627.1\t72\t83\t72.00\t80.15") },
                 { assert snapshot(
-                    path(process.out.tsv[0][1]).exists(),
-                    path(process.out.abridged_tsv[0][1]).exists(),
+                    file(process.out.tsv[0][1]).name,
                     process.out.out_gtf,
-                    path(process.out.log[0][1]).exists(),
+                    file(process.out.abridged_tsv[0][1]).name,
+                    file(process.out.log[0][1]).name,
+                    process.out.versions
                 ).match() }
             )
         }
 
     }
 
-    test("FUSIONINSPECTOR stub") {
+    test("FUSIONINSPECTOR - test - stub") {
         options "-stub"
         when {
             process {
@@ -80,8 +85,8 @@ nextflow_process {
                 input[0] = [
                     [ id:'test', single_end:false ], // meta map
                     [
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_1.fastq.gz'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_2.fastq.gz')
                     ], // reads
                     [
                         file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")
diff --git a/modules/nf-core/fusioninspector/tests/main.nf.test.snap b/modules/nf-core/fusioninspector/tests/main.nf.test.snap
new file mode 100644
index 000000000..4a8d000ee
--- /dev/null
+++ b/modules/nf-core/fusioninspector/tests/main.nf.test.snap
@@ -0,0 +1,199 @@
+{
+    "FUSIONINSPECTOR - test": {
+        "content": [
+            "test.FusionInspector.fusions.tsv",
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.gtf:md5,d523061bd8b443a014d4cca9406ec772"
+                ]
+            ],
+            "test.FusionInspector.fusions.abridged.tsv",
+            "FusionInspector.log",
+            [
+                "versions.yml:md5,7c9694f4a2d8edbd30fd9674566b764c"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-07T13:48:15.295143368"
+    },
+    "FUSIONINSPECTOR - test - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusion_inspector_web.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "8": [
+                    "versions.yml:md5,7c9694f4a2d8edbd30fd9674566b764c"
+                ],
+                "abridged_tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "chckpts_dir": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "fi_workdir": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        ]
+                    ]
+                ],
+                "html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fusion_inspector_web.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "igv_inputs": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            
+                        ]
+                    ]
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "FusionInspector.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "out_gtf": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "tsv": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.FusionInspector.fusions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,7c9694f4a2d8edbd30fd9674566b764c"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-18T20:42:22.142466729"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/fusioninspector/tests/nextflow.config b/modules/nf-core/fusioninspector/tests/nextflow.config
new file mode 100644
index 000000000..ca61431b2
--- /dev/null
+++ b/modules/nf-core/fusioninspector/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'FUSIONINSPECTOR' {
+        ext.args = '--annotate --examine_coding_effect'
+    }
+}

From 0c1c493358de3f4380e4afad35d2a17f8183ade6 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 12:26:29 +0000
Subject: [PATCH 759/887] chore: replace local for nf-core modules

---
 subworkflows/local/build_references/main.nf    | 2 +-
 subworkflows/local/fusioninspector_workflow.nf | 4 ++--
 workflows/rnafusion.nf                         | 2 +-
 3 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index eecec62ef..d71369070 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -7,13 +7,13 @@
 include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode_download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
 include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
-include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/local/ctatsplicing/prepgenomelib/main.nf'
 
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
+include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/nf-core/ctatsplicing/prepgenomelib/main.nf'
 include { BIOAWK                          } from '../../../modules/nf-core/bioawk/main'
 include { AGAT_CONVERTGFF2BED             } from '../../../modules/nf-core/agat/convertgff2bed/main'
 include { SAMTOOLS_FAIDX }                  from '../../../modules/nf-core/samtools/faidx/main'
diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf
index c54b3c7b4..39ac4144a 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow.nf
@@ -1,8 +1,8 @@
 include { AGAT_CONVERTSPGFF2TSV     }                     from '../../modules/nf-core/agat/convertspgff2tsv/main'
-include { ARRIBA_VISUALISATION     }                      from '../../modules/local/arriba/visualisation/main'
+include { ARRIBA_VISUALISATION     }                      from '../../modules/nf-core/arriba/visualisation/main'
 include { CAT_CAT }                                       from '../../modules/nf-core/cat/cat/main'
 include { VCF_COLLECT }                                   from '../../modules/local/vcf_collect/main'
-include { FUSIONINSPECTOR     }                           from '../../modules/local/fusioninspector/main'
+include { FUSIONINSPECTOR     }                           from '../../modules/nf-core/fusioninspector/main'
 
 workflow FUSIONINSPECTOR_WORKFLOW {
     take:
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 2bb866c21..8906891b9 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -25,7 +25,7 @@ include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_
 include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { validateInputSamplesheet      }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
 include { ARRIBA_ARRIBA                 }   from '../modules/nf-core/arriba/arriba/main'
-include { CTATSPLICING_STARTOCANCERINTRONS } from '../modules/local/ctatsplicing/startocancerintrons'
+include { CTATSPLICING_STARTOCANCERINTRONS } from '../modules/nf-core/ctatsplicing/startocancerintrons'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From ed7d88d5fedd7e7cd0df508a2f2012d4a5ef04f5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 8 Aug 2025 14:29:19 +0200
Subject: [PATCH 760/887] update snape

---
 modules/local/fusioninspector/tests/main.nf.test.snap | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/modules/local/fusioninspector/tests/main.nf.test.snap b/modules/local/fusioninspector/tests/main.nf.test.snap
index 79e97ea6e..88bb1c172 100644
--- a/modules/local/fusioninspector/tests/main.nf.test.snap
+++ b/modules/local/fusioninspector/tests/main.nf.test.snap
@@ -44,7 +44,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.FusionInspector.fusions.abridged.tsv:md5,23ea1445500aaa94f943bade166f39f9"
                     ]
                 ],
                 "5": [
@@ -198,7 +198,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.FusionInspector.fusions.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                        "test.FusionInspector.fusions.abridged.tsv:md5,23ea1445500aaa94f943bade166f39f9"
                     ]
                 ],
                 "chckpts_dir": [
@@ -386,9 +386,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-30T15:11:46.208438"
+        "timestamp": "2025-08-08T10:21:25.946458"
     },
     "FUSIONINSPECTOR": {
         "content": [

From 777073c98852313106eff680cfc538fa1c3e5a55 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 12:31:20 +0000
Subject: [PATCH 761/887] chore: replace local for nf-core starfusion detect

---
 modules/local/starfusion/detect/main.nf       |  56 -----
 modules/local/starfusion/detect/meta.yml      |  56 -----
 .../starfusion/detect/tests/main.nf.test      |  96 ---------
 .../starfusion/detect/tests/main.nf.test.snap | 104 ---------
 .../starfusion/detect/environment.yml         |   0
 modules/nf-core/starfusion/detect/main.nf     |  60 ++++++
 modules/nf-core/starfusion/detect/meta.yml    |  80 +++++++
 .../starfusion/detect/tests/main.nf.test      | 148 +++++++++++++
 .../starfusion/detect/tests/main.nf.test.snap | 197 ++++++++++++++++++
 .../starfusion/detect/tests/nextflow.config   |   5 +
 10 files changed, 490 insertions(+), 312 deletions(-)
 delete mode 100644 modules/local/starfusion/detect/main.nf
 delete mode 100644 modules/local/starfusion/detect/meta.yml
 delete mode 100644 modules/local/starfusion/detect/tests/main.nf.test
 delete mode 100644 modules/local/starfusion/detect/tests/main.nf.test.snap
 rename modules/{local => nf-core}/starfusion/detect/environment.yml (100%)
 create mode 100644 modules/nf-core/starfusion/detect/main.nf
 create mode 100644 modules/nf-core/starfusion/detect/meta.yml
 create mode 100644 modules/nf-core/starfusion/detect/tests/main.nf.test
 create mode 100644 modules/nf-core/starfusion/detect/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/starfusion/detect/tests/nextflow.config

diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf
deleted file mode 100644
index f74d26313..000000000
--- a/modules/local/starfusion/detect/main.nf
+++ /dev/null
@@ -1,56 +0,0 @@
-process STARFUSION {
-    tag "$meta.id"
-    label 'process_high'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/75/75d085bf2a8e40c6693b357800eef0f9568f661226d0888339bc77f7852234bb/data' :
-        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:e285bb3eb373b9a7'}"
-
-    input:
-    tuple val(meta), path(reads), path(junction)
-    path reference
-
-    output:
-    tuple val(meta), path("*.fusion_predictions.tsv")                   , emit: fusions
-    tuple val(meta), path("*.abridged.tsv")                             , emit: abridged
-    tuple val(meta), path("*.coding_effect.tsv")     , optional: true   , emit: coding_effect
-    path "versions.yml"                                                 , emit: versions
-
-    script:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def fastq_arg = reads ? (meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}") : ""
-    def junction_arg =  junction ? "-J ${junction}" : ""
-    def args = task.ext.args ?: ''
-    """
-    STAR-Fusion \\
-        --genome_lib_dir $reference \\
-        $fastq_arg \\
-        $junction_arg \\
-        --CPU $task.cpus \\
-        --examine_coding_effect \\
-        --output_dir . \\
-        $args
-
-    mv star-fusion.fusion_predictions.tsv ${prefix}.starfusion.fusion_predictions.tsv
-    mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.starfusion.abridged.tsv
-    mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${prefix}.starfusion.abridged.coding_effect.tsv
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
-    END_VERSIONS
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    touch ${prefix}.starfusion.fusion_predictions.tsv
-    touch ${prefix}.starfusion.abridged.tsv
-    touch ${prefix}.starfusion.abridged.coding_effect.tsv
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        STAR-Fusion: \$(STAR-Fusion --version 2>&1 | grep -i 'version' | sed 's/STAR-Fusion version: //')
-    END_VERSIONS
-    """
-}
diff --git a/modules/local/starfusion/detect/meta.yml b/modules/local/starfusion/detect/meta.yml
deleted file mode 100644
index 7337dad5c..000000000
--- a/modules/local/starfusion/detect/meta.yml
+++ /dev/null
@@ -1,56 +0,0 @@
-name: starfusion
-description: Fast and Accurate Fusion Transcript Detection from RNA-Seq
-keywords:
-  - Fusion
-tools:
-  - star-fusion:
-      description: Fast and Accurate Fusion Transcript Detection from RNA-Seq
-      homepage: https://github.com/STAR-Fusion/STAR-Fusion
-      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki
-      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion/releases
-      doi: "10.1101/120295v1"
-      licence: ["GPL v3"]
-
-input:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - genome_lib:
-      type: path
-      description: STAR-fusion reference genome lib folder
-  - junction:
-      type: file
-      description: Chimeric junction output from STAR aligner
-      pattern: "*.{out.junction}"
-  - reference:
-      type: directory
-      description: Reference dir
-      pattern: "ctat_genome_lib_build_dir"
-
-output:
-  - meta:
-      type: map
-      description: |
-        Groovy Map containing sample information
-        e.g. [ id:'test', single_end:false ]
-  - version:
-      type: file
-      description: File containing software version
-      pattern: "*.{versions.yml}"
-  - fusions:
-      type: file
-      description: Fusion events from STAR-fusion
-      pattern: "*.{fusion_predictions.tsv}"
-  - abridged:
-      type: file
-      description: Fusion events from STAR-fusion
-      pattern: "*.{fusion.abridged.tsv}"
-  - coding_effect:
-      type: file
-      description: Fusion events from STAR-fusion
-      pattern: "*.{coding_effect.tsv}"
-
-authors:
-  - "@praveenraj2018"
diff --git a/modules/local/starfusion/detect/tests/main.nf.test b/modules/local/starfusion/detect/tests/main.nf.test
deleted file mode 100644
index 2dec2d2cf..000000000
--- a/modules/local/starfusion/detect/tests/main.nf.test
+++ /dev/null
@@ -1,96 +0,0 @@
-nextflow_process {
-
-    name "Test Process STARFUSION"
-    script "../main.nf"
-    process "STARFUSION"
-
-    setup {
-        run("STARFUSION_BUILD") {
-            script "../../../../nf-core/starfusion/build/main.nf"
-            process {
-                """
-                input[0] = [
-                    [ id:'minigenome_fasta' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
-                ]
-                input[1] = [
-                    [ id:'minigenome_gtf' ],
-                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
-                ]
-
-                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
-                input[3] = "homo_sapiens"
-                input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
-                input[5] = [
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
-                    "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
-                ]
-                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
-                """
-            }
-        }
-    }
-
-    test("Should run without failures") {
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test', single_end:false ], // meta map
-                    [
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
-                    ], // reads
-                    [] // empty list for junction, as we don't have a pre-computed Chimeric.out.junction file
-                ]
-                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
-                """
-            }
-        }
-
-        then {
-            assert process.success
-            assert snapshot(
-                process.out.fusions,
-                process.out.abridged,
-                path(process.out.coding_effect[0][1]).resolve("*.coding_effect.tsv").exists(),
-                process.out.versions
-            ).match()
-        }
-
-    }
-
-    test("Should create stub files") {
-
-        options "-stub"
-
-        when {
-            process {
-                """
-                input[0] = [
-                    [ id:'test_stub', single_end:false ],
-                    [
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
-                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
-                    ],
-                    [] // empty list for junction
-                ]
-                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
-                """
-            }
-        }
-
-        then {
-            assert process.success
-            assert snapshot(
-                process.out
-            ).match()
-        }
-
-    }
-
-}
diff --git a/modules/local/starfusion/detect/tests/main.nf.test.snap b/modules/local/starfusion/detect/tests/main.nf.test.snap
deleted file mode 100644
index 3fe34cbc2..000000000
--- a/modules/local/starfusion/detect/tests/main.nf.test.snap
+++ /dev/null
@@ -1,104 +0,0 @@
-{
-    "Should create stub files": {
-        "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test_stub",
-                            "single_end": false
-                        },
-                        "test_stub.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "1": [
-                    [
-                        {
-                            "id": "test_stub",
-                            "single_end": false
-                        },
-                        "test_stub.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "2": [
-                    [
-                        {
-                            "id": "test_stub",
-                            "single_end": false
-                        },
-                        "test_stub.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "3": [
-                    "versions.yml:md5,1597cbbd06bf7278faa9c6282665cbac"
-                ],
-                "abridged": [
-                    [
-                        {
-                            "id": "test_stub",
-                            "single_end": false
-                        },
-                        "test_stub.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "coding_effect": [
-                    [
-                        {
-                            "id": "test_stub",
-                            "single_end": false
-                        },
-                        "test_stub.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "fusions": [
-                    [
-                        {
-                            "id": "test_stub",
-                            "single_end": false
-                        },
-                        "test_stub.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,1597cbbd06bf7278faa9c6282665cbac"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-22T16:26:57.806399"
-    },
-    "Should run without failures": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.starfusion.fusion_predictions.tsv:md5,82834fffed743afe07da82bd56d50c99"
-                ]
-            ],
-            [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.starfusion.abridged.tsv:md5,d6d20fdd4b5cba21b9c0ebf8e0ea19ff"
-                ]
-            ],
-            false,
-            [
-                "versions.yml:md5,1597cbbd06bf7278faa9c6282665cbac"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-26T21:15:38.318797"
-    }
-}
\ No newline at end of file
diff --git a/modules/local/starfusion/detect/environment.yml b/modules/nf-core/starfusion/detect/environment.yml
similarity index 100%
rename from modules/local/starfusion/detect/environment.yml
rename to modules/nf-core/starfusion/detect/environment.yml
diff --git a/modules/nf-core/starfusion/detect/main.nf b/modules/nf-core/starfusion/detect/main.nf
new file mode 100644
index 000000000..5211a3100
--- /dev/null
+++ b/modules/nf-core/starfusion/detect/main.nf
@@ -0,0 +1,60 @@
+process STARFUSION_DETECT {
+    tag "$meta.id"
+    label 'process_high'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/75/75d085bf2a8e40c6693b357800eef0f9568f661226d0888339bc77f7852234bb/data' :
+        'community.wave.seqera.io/library/dfam_hmmer_minimap2_star-fusion:e285bb3eb373b9a7'}"
+
+    input:
+    tuple val(meta), path(reads), path(junction)
+    path reference
+
+    output:
+    tuple val(meta), path("*.fusion_predictions.tsv"), emit: fusions
+    tuple val(meta), path("*.abridged.tsv")          , emit: abridged
+    tuple val(meta), path("*.coding_effect.tsv")     , emit: coding_effect, optional: true
+    path "versions.yml"                              , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def prefix       = task.ext.prefix ?: "${meta.id}.starfusion"
+    def fastq_arg    = reads ? (meta.single_end ? "--left_fq ${reads[0]}" : "--left_fq ${reads[0]} --right_fq ${reads[1]}") : ""
+    def junction_arg =  junction ? "-J ${junction}" : ""
+    def args         = task.ext.args ?: ''
+    def VERSION      = '1.15.1' // WARN: This is the actual version of the STAR-FUSION, but version information of tool is not updated and prints '1.15.0'
+    """
+    STAR-Fusion \\
+        --genome_lib_dir $reference \\
+        $fastq_arg \\
+        $junction_arg \\
+        --CPU $task.cpus \\
+        --output_dir . \\
+        $args
+
+    mv star-fusion.fusion_predictions.tsv ${prefix}.fusion_predictions.tsv
+    mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.abridged.tsv
+    mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${prefix}.abridged.coding_effect.tsv
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        STAR-Fusion: $VERSION
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix  = task.ext.prefix ?: "${meta.id}.starfusion"
+    def VERSION = '1.15.1'
+    """
+    touch ${prefix}.fusion_predictions.tsv
+    touch ${prefix}.abridged.tsv
+    touch ${prefix}.abridged.coding_effect.tsv
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        STAR-Fusion: $VERSION
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/starfusion/detect/meta.yml b/modules/nf-core/starfusion/detect/meta.yml
new file mode 100644
index 000000000..455981efe
--- /dev/null
+++ b/modules/nf-core/starfusion/detect/meta.yml
@@ -0,0 +1,80 @@
+name: starfusion_detect
+description: Fast and Accurate Fusion Transcript Detection from RNA-Seq
+keywords:
+  - Fusion
+  - starfusion
+  - RNA-Seq
+  - detect
+tools:
+  - star-fusion:
+      description: Fast and Accurate Fusion Transcript Detection from RNA-Seq
+      homepage: https://github.com/STAR-Fusion/STAR-Fusion
+      documentation: https://github.com/STAR-Fusion/STAR-Fusion/wiki
+      tool_dev_url: https://github.com/STAR-Fusion/STAR-Fusion/releases
+      doi: "10.1101/120295v1"
+      licence: ["GPL v3"]
+      identifier: ""
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: file
+        description: Input fastq files
+        pattern: "*.{fq,fastq,fq.gz,fastq.gz}"
+        ontologies:
+          - edam: "http://edamontology.org/format_1930" # FASTQ
+    - junction:
+        type: file
+        description: Chimeric junction output from STAR aligner
+        pattern: "*.{out.junction}"
+        ontologies: []
+  - reference:
+      type: directory
+      description: STAR-fusion reference genome lib folder
+      pattern: "*genome_lib_build_dir"
+
+output:
+  fusions:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fusion_predictions.tsv":
+          type: file
+          description: Fusion events from STAR-fusion
+          pattern: "*.{fusion_predictions.tsv}"
+  abridged:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.abridged.tsv":
+          type: file
+          description: Abridged version of fusion events from STAR-fusion
+          pattern: "*.{fusion.abridged.tsv}"
+  coding_effect:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.coding_effect.tsv":
+          type: file
+          description: Fusion events with their coding effect from STAR-fusion
+          pattern: "*.{coding_effect.tsv}"
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: "http://edamontology.org/format_3750" # YAML
+
+authors:
+  - "@praveenraj2018"
diff --git a/modules/nf-core/starfusion/detect/tests/main.nf.test b/modules/nf-core/starfusion/detect/tests/main.nf.test
new file mode 100644
index 000000000..7c2c24d68
--- /dev/null
+++ b/modules/nf-core/starfusion/detect/tests/main.nf.test
@@ -0,0 +1,148 @@
+nextflow_process {
+
+    name "Test Process STARFUSION_DETECT"
+    script "../main.nf"
+    process "STARFUSION_DETECT"
+    tag "modules_nfcore"
+    tag "starfusion/build"
+    tag "starfusion"
+    tag "starfusion/detect"
+    tag "modules"
+    config "./nextflow.config"
+
+    setup {
+        run("STARFUSION_BUILD") {
+            script "../../../../nf-core/starfusion/build/main.nf"
+            process {
+                """
+                input[0] = [
+                    [ id:'minigenome_fasta' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
+                ]
+                input[1] = [
+                    [ id:'minigenome_gtf' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
+                ]
+
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                input[3] = "homo_sapiens"
+                input[4] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/Pfam-A.hmm.gz')
+                input[5] = [
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3f'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3i'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3m'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3p')
+                ]
+                input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                """
+            }
+        }
+    }
+
+    test("Paired end - Should run without failures") {
+
+        when {
+            params {
+                starfusion_detect_args = "--examine_coding_effect"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_1.fastq.gz'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_2.fastq.gz')
+                    ], // reads
+                    [] // empty list for junction, as we don't have a pre-computed Chimeric.out.junction file
+                ]
+                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
+                """
+            }
+        }
+
+        then {
+            assert process.success
+            assert snapshot(
+                process.out.fusions,
+                process.out.abridged,
+                process.out.versions,
+                process.out.coding_effect.collect { meta, tsv ->
+                    [meta, path(tsv).readLines().collect { line ->
+                        line.split('\t')[0] // Get first column (tab-separated)
+                    }]
+                }
+            ).match()
+        }
+
+    }
+
+    test("Single end - Should run without failures") {
+        tag "single"
+        when {
+            params {
+                starfusion_detect_args = "--examine_coding_effect"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test', single_end:true ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_1.fastq.gz')
+                    ], // reads
+                    [] // empty list for junction, as we don't have a pre-computed Chimeric.out.junction file
+                ]
+                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
+                """
+            }
+        }
+
+        then {
+            assert process.success
+            assert snapshot(
+                process.out.fusions,
+                process.out.abridged,
+                process.out.versions,
+                process.out.coding_effect.collect { meta, tsv ->
+                    [meta, path(tsv).readLines().collect { line ->
+                        line.split('\t')[0] // Get first column (tab-separated)
+                    }]
+                }
+            ).match()
+        }
+
+    }
+
+    test("Should create stub files") {
+
+        options "-stub"
+
+        when {
+            params {
+                starfusion_detect_args = "--examine_coding_effect"
+            }
+            process {
+                """
+                input[0] = [
+                    [ id:'test_stub', single_end:false ],
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_1.fastq.gz'),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_rnaseq_2.fastq.gz')
+                    ],
+                    [] // empty list for junction
+                ]
+                input[1] = STARFUSION_BUILD.out.reference.map { it[1] }
+                """
+            }
+        }
+
+        then {
+            assert process.success
+            assert snapshot(
+                process.out
+            ).match()
+        }
+
+    }
+
+}
\ No newline at end of file
diff --git a/modules/nf-core/starfusion/detect/tests/main.nf.test.snap b/modules/nf-core/starfusion/detect/tests/main.nf.test.snap
new file mode 100644
index 000000000..b497edcff
--- /dev/null
+++ b/modules/nf-core/starfusion/detect/tests/main.nf.test.snap
@@ -0,0 +1,197 @@
+{
+    "Should create stub files": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,a21fed36ad3c5144d6a06d48d34f5a2b"
+                ],
+                "abridged": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "coding_effect": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.abridged.coding_effect.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fusions": [
+                    [
+                        {
+                            "id": "test_stub",
+                            "single_end": false
+                        },
+                        "test_stub.starfusion.fusion_predictions.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,a21fed36ad3c5144d6a06d48d34f5a2b"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-29T17:50:20.401704096"
+    },
+    "Paired end - Should run without failures": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.starfusion.fusion_predictions.tsv:md5,82834fffed743afe07da82bd56d50c99"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.starfusion.abridged.tsv:md5,d6d20fdd4b5cba21b9c0ebf8e0ea19ff"
+                ]
+            ],
+            [
+                "versions.yml:md5,a21fed36ad3c5144d6a06d48d34f5a2b"
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "#FusionName",
+                        "TATDN1--GSDMB",
+                        "ACACA--STAC2",
+                        "TATDN1--GSDMB",
+                        "CCDC6--RET",
+                        "TOB1--SYNRG",
+                        "TATDN1--GSDMB",
+                        "BCAS4--BCAS3",
+                        "RPS6KB1--SNF8",
+                        "BAG6--SLC44A4",
+                        "BCR--ABL1",
+                        "VAPB--IKZF3",
+                        "CYTH1--EIF3H",
+                        "RAB22A--MYO9B",
+                        "RARA--PKIA",
+                        "ARFGEF2--SULF2",
+                        "SUMF1--LRRFIP2",
+                        "ANKHD1--PCDH1",
+                        "MED1--ACSF2",
+                        "AHCTF1--NAAA",
+                        "MED1--STXBP4",
+                        "BCAS4--BCAS3",
+                        "ZMYND8--CEP250",
+                        "AC007562.1--AC012005.2",
+                        "VAPB--IKZF3",
+                        "SUMF1--LRRFIP2",
+                        "ZMYND8--CEP250",
+                        "RARA--PKIA",
+                        "CYTH1--EIF3H",
+                        "MED13--BCAS3",
+                        "VAPB--IKZF3"
+                    ]
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-29T17:14:52.628015363"
+    },
+    "Single end - Should run without failures": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.starfusion.fusion_predictions.tsv:md5,62edb2285932b5afd88f6aba7d4ebdd1"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.starfusion.abridged.tsv:md5,4f76c050e3b012320b5e526f7076ffb8"
+                ]
+            ],
+            [
+                "versions.yml:md5,a21fed36ad3c5144d6a06d48d34f5a2b"
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    [
+                        "#FusionName",
+                        "CCDC6--RET",
+                        "TATDN1--GSDMB",
+                        "TOB1--SYNRG",
+                        "ACACA--STAC2",
+                        "TATDN1--GSDMB",
+                        "BAG6--SLC44A4",
+                        "TATDN1--GSDMB",
+                        "RARA--PKIA",
+                        "AC007562.1--AC012005.2",
+                        "SUMF1--LRRFIP2",
+                        "RAB22A--MYO9B",
+                        "BCR--ABL1"
+                    ]
+                ]
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-07-29T17:42:24.763176482"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/starfusion/detect/tests/nextflow.config b/modules/nf-core/starfusion/detect/tests/nextflow.config
new file mode 100644
index 000000000..dd8a78dba
--- /dev/null
+++ b/modules/nf-core/starfusion/detect/tests/nextflow.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'STARFUSION_DETECT' {
+        ext.args = params.starfusion_detect_args
+    }
+}

From 66b67b181d74949217efed131ecc429effdf9836 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 12:33:27 +0000
Subject: [PATCH 762/887] chore: replace local for nf-core starfusion detect

---
 workflows/rnafusion.nf | 8 ++++----
 1 file changed, 4 insertions(+), 4 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 8906891b9..beccf1c93 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -8,7 +8,7 @@ include { BUILD_REFERENCES              }   from '../subworkflows/local/build_re
 include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
 include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow/main'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
-include { STARFUSION                    }   from '../modules/local/starfusion/detect/main'
+include { STARFUSION_DETECT             }   from '../modules/nf-core/starfusion/detect/main'
 include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
 include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
@@ -258,12 +258,12 @@ workflow RNAFUSION {
                 def fusions = file(params.starfusion_fusions, checkIfExists:true)
                 ch_starfusion_fusions = ch_star_junctions.map { meta, _junc -> [ meta, fusions ] }
             } else {
-                STARFUSION(
+                STARFUSION_DETECT(
                     ch_star_junctions.map { meta, junc -> [ meta, [], junc ] },
                     BUILD_REFERENCES.out.starfusion_ref.map { it -> it[1] }
                 )
-                ch_versions = ch_versions.mix(STARFUSION.out.versions)
-                ch_starfusion_fusions = STARFUSION.out.fusions
+                ch_versions = ch_versions.mix(STARFUSION_DETECT.out.versions)
+                ch_starfusion_fusions = STARFUSION_DETECT.out.fusions
             }
         }
 

From e05eaf3e5a1fbdb85898dc8813db220e657eda10 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 8 Aug 2025 14:40:44 +0200
Subject: [PATCH 763/887] update usage.md

---
 docs/usage.md | 32 +++++++++++++++-----------------
 1 file changed, 15 insertions(+), 17 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 16228338d..41aa0299e 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -155,9 +155,9 @@ TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,forward
 | `seq_platform`     | The sequencing platform used to create to sequence the data in the FASTQ files. This value will take precedence over the value provided with `--seq_platform`.                                                                                                                                                                                                                                                                                | ❌                |
 | `seq_center`       | The sequencing center in which the data in the FASTQ files was sequenced. This value will take precedence over the value provided with `--seq_center`.                                                                                                                                                                                                                                                                                        | ❌                |
 
-✅ = Required
-❌ = Not required
-❓ = One of these columns should be provided
+- ✅ = Required
+- ❌ = Not required
+- ❓ = One of these columns should be provided
 
 ### Preparing BAM/CRAM/junctions/splice_junctions
 
@@ -198,7 +198,7 @@ The pipeline will still work when another command has been used, but it is possi
 
 ### Starting commands
 
-The pipeline can either be run using all fusion detection tools or by specifying individual tools. Visualisation tools will be run on all fusions detected. To run all tools (`arriba`, `ctatsplicing`, `fusioncatcher`, `starfusion`, `stringtie`, `fusionreport`, `fastp`, `salmon`, `fusioninspector`) use the `all` option for the `--tools` parameter:
+The pipeline can either be run using all tools or by specifying individual tools. Visualisation tools will be run on all fusions detected. To run all tools (`arriba`, `ctatsplicing`, `fusioncatcher`, `starfusion`, `stringtie`, `fusionreport`, `fastp`, `salmon`, `fusioninspector`) use the `all` option for the `--tools` parameter:
 
 ```bash
 nextflow run nf-core/rnafusion \
@@ -224,7 +224,7 @@ If you are not covered by the research COSMIC license and want to avoid using CO
 
 > **IMPORTANT: `--tools`** is necessary to run detection tools
 
-`--genomes_base` should be the path to the directory containing the folder `references/` that was built with `--references_only`.
+`--genomes_base` should be the path to the directory containing the references that were [downloaded or built](#download-and-build-references).
 
 Note that the pipeline will create the following files in your working directory:
 
@@ -258,16 +258,15 @@ outdir: './results/'
 
 You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch).
 
-:::warning
-Conda is not currently supported.
-Supported genome is currently only GRCh38.
-:::
+> [!WARNING]
+> Conda is not currently supported.
+> Supported genome is currently only GRCh38.
 
 ### Options
 
 #### Trimming
 
-When the flag `fastp` tool is used in `--tools`, `fastp` is used to provide all tools with trimmed reads. Quality and adapter trimming by default. In addition, tail trimming and adapter_fastq specification are possible. Example usage:
+When the flag `fastp` tool is used in `--tools`, `fastp` is used to provide all tools with trimmed reads. Quality and adapter trimming are performed by default. In addition, tail trimming for fusioncatcher and adapter fasta specification are possible. Example usage:
 
 ```bash
 nextflow run nf-core/rnafusion \
@@ -277,11 +276,11 @@ nextflow run nf-core/rnafusion \
 --genomes_base <PATH/TO/REFERENCES> \
 --outdir <OUTPUT/PATH> \
 --fastp_trim \
---trim_tail <INTEGER> (optional) \
---adapter_fastq <PATH/TO/ADAPTER/FASTQ> (optional)
+--trim_tail_fusioncatcher <INTEGER> (optional) \
+--adapter_fasta <PATH/TO/ADAPTER/FASTA> (optional)
 ```
 
-The additional `--trim_tail_fusioncatcher` flag will toggle an additional `fastp` process, especially useful is reads are above 100 bp, which is not handled well by FusionCatcher. The parameter `--trim_tail_fusioncatcher`need to be provided with the number of bases to remove from the tail end.
+The additional `--trim_tail_fusioncatcher` flag will toggle an additional `fastp` process, especially useful is reads are above 100 bp, which is not handled well by FusionCatcher. The parameter `--trim_tail_fusioncatcher` needs to be provided with the number of bases to remove from the tail end.
 
 #### Filter fusions detected by 2 or more tools
 
@@ -378,10 +377,9 @@ There are two parameters to increase the `--limitSjdbInsertNsj` parameter if nec
 - `--fusioncatcher_limitSjdbInsertNsj`, default: 2000000
 - `--fusioninspector_limitSjdbInsertNsj`, default: 1000000
 
-Use the parameter `--cram` to compress the BAM files to CRAM for specific tools. Options: arriba, starfusion. Leave no space between options:
+#### CRAM compression
 
-- `--cram arriba,starfusion`, default: []
-- `--cram arriba`
+Use the parameter `--cram` to compress the BAM files to CRAM.
 
 ### Troubleshooting
 
@@ -398,7 +396,7 @@ As the error message suggests, it is a STAR-related error and your best luck in
 
 ### Updating the pipeline
 
-When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
+When you run the command below, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
 
 ```bash
 nextflow pull nf-core/rnafusion

From dae121c95c609adb4fd82caa7f2619becd489c55 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 8 Aug 2025 14:44:28 +0200
Subject: [PATCH 764/887] pre-commit

---
 docs/usage.md | 30 +++++++++++++++---------------
 1 file changed, 15 insertions(+), 15 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 41aa0299e..8d3ca0d3f 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -124,7 +124,7 @@ The references are only built based on ensembl version 102. It is not possible c
 
 ### Samplesheet input
 
-You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. The pipeline will detect whether a sample is single- or paired-end from the samplesheet - the `fastq_2` column is empty for single-end. 
+You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. The pipeline will detect whether a sample is single- or paired-end from the samplesheet - the `fastq_2` column is empty for single-end.
 
 The samplesheet has to be a comma-separated (.csv), tab-separated (.tsv), yaml (.yaml or .yml) or json (.json) file but can have as many entries as you desire. There is a strict requirement for the `sample` and `strandedness` fields. One or more of these fields should be provided too: `fastq_1`, `bam`, `cram`, `junctions` and `splice_junctions`
 
@@ -140,20 +140,20 @@ TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,,forward
 TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,forward
 ```
 
-| Column             | Description                                                                                                                                                                                                                                                                                                                                                                                                                                   | Required           |
-| ------------------ | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------ |
-| `sample`           | Custom sample name. This value needs to be unique across all entries in the samplesheet and cannot contain spaces                                                                                                                                                                                                                                                     | ✅ |
-| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                                                                                                             | ✅ |
-| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.                                                                                      | ❓    |
-| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                      | ❓    |
-| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.   | ❓    |
-| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                                                                                                                 | ❌                |
-| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty. | ❓    |
-| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                                                                                                               | ❌                |
-| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.      | ❓    |
-| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                | ❓    |
-| `seq_platform`     | The sequencing platform used to create to sequence the data in the FASTQ files. This value will take precedence over the value provided with `--seq_platform`.                                                                                                                                                                                                                                                                                | ❌                |
-| `seq_center`       | The sequencing center in which the data in the FASTQ files was sequenced. This value will take precedence over the value provided with `--seq_center`.                                                                                                                                                                                                                                                                                        | ❌                |
+| Column             | Description                                                                                                                                                                                                                                                                                                                                                                                                                                   | Required |
+| ------------------ | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------- |
+| `sample`           | Custom sample name. This value needs to be unique across all entries in the samplesheet and cannot contain spaces                                                                                                                                                                                                                                                                                                                             | ✅       |
+| `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                                                                                                             | ✅       |
+| `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.                                                                                      | ❓       |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                      | ❓       |
+| `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.   | ❓       |
+| `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                                                                                                                 | ❌       |
+| `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty. | ❓       |
+| `crai`             | Full path to the index of the CRAM file. File has to exist and must have the extension ".crai".                                                                                                                                                                                                                                                                                                                                               | ❌       |
+| `junctions`        | Full path to the file containing chimeric junctions determined by STAR. File has to exist and must have the extension ".junction". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The junctions file is required to run `starfusion` and `ctatsplicing` when the `fastq_1` field is empty.      | ❓       |
+| `splice_junctions` | Full path to the file containing splice junctions determined by STAR. File has to exist and must have the extension ".SJ.out.tab". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The splice junctions file is required to run `ctatsplicing` when the `fastq_1` field is empty.                | ❓       |
+| `seq_platform`     | The sequencing platform used to create to sequence the data in the FASTQ files. This value will take precedence over the value provided with `--seq_platform`.                                                                                                                                                                                                                                                                                | ❌       |
+| `seq_center`       | The sequencing center in which the data in the FASTQ files was sequenced. This value will take precedence over the value provided with `--seq_center`.                                                                                                                                                                                                                                                                                        | ❌       |
 
 - ✅ = Required
 - ❌ = Not required

From 3d06bc8119930d0c482ed44ef9da6b19c9a9c4b4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Fri, 8 Aug 2025 14:48:06 +0200
Subject: [PATCH 765/887] nf-core lint

---
 ro-crate-metadata.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 2d97d3a9e..68b7f82a0 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 5 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n:warning: **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only genocode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Create [STAR](https://github.com/alexdobin/STAR) index\n3. Download [Arriba](https://github.com/suhrig/arriba) references\n4. Download [FusionCatcher](https://github.com/ndaniel/fusioncatcher) references\n5. Download and build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) references\n6. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Concatenate fastq files per sample ([cat](http://www.linfo.org/cat.html))\n3. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n4. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n5. Arriba subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [Arriba](https://github.com/suhrig/arriba) fusion detection\n6. STAR-fusion subworkflow\n   - [STAR](https://github.com/alexdobin/STAR) alignment\n   - [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) fusion detection\n7. Fusioncatcher subworkflow\n   - [FusionCatcher](https://github.com/ndaniel/fusioncatcher) fusion detection\n8. StringTie subworkflow\n   - [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Fusion-report\n   - Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`picard MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), it is recommended to test the pipeline with the `-stub` parameter (creation of empty files):\n\nFirst, build the references:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   --references_only \\\n   -stub\n```\n\nThen perform the analysis:\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR>\\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n7. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n8. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n11. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n12. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n13. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"

From 5d29378c746f7c34e7426ed74fd60191092ea0c8 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 14:02:01 +0000
Subject: [PATCH 766/887] chore: import nf-core modules

---
 modules.json | 30 +++++++++++++++++++++++++-----
 1 file changed, 25 insertions(+), 5 deletions(-)

diff --git a/modules.json b/modules.json
index 1ab893088..a4cd21862 100644
--- a/modules.json
+++ b/modules.json
@@ -20,6 +20,11 @@
                         "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
                         "installed_by": ["modules"]
                     },
+                    "arriba/visualisation": {
+                        "branch": "master",
+                        "git_sha": "025a6f75e1f72e4ab60abb4bd65b3f289d4ad910",
+                        "installed_by": ["modules"]
+                    },
                     "bioawk": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
@@ -35,6 +40,16 @@
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["modules"]
                     },
+                    "ctatsplicing/prepgenomelib": {
+                        "branch": "master",
+                        "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
+                        "installed_by": ["modules"]
+                    },
+                    "ctatsplicing/startocancerintrons": {
+                        "branch": "master",
+                        "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
+                        "installed_by": ["modules"]
+                    },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
@@ -50,6 +65,11 @@
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
+                    "fusioninspector": {
+                        "branch": "master",
+                        "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
+                        "installed_by": ["modules"]
+                    },
                     "fusionreport/detect": {
                         "branch": "master",
                         "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
@@ -100,11 +120,6 @@
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
                         "installed_by": ["modules"]
                     },
-                    "rrnatranscripts": {
-                        "branch": "master",
-                        "git_sha": "b785bb0b56bb518ab40c41f41d0e8749fb638257",
-                        "installed_by": ["modules"]
-                    },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
@@ -150,6 +165,11 @@
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
+                    "starfusion/detect": {
+                        "branch": "master",
+                        "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
+                        "installed_by": ["modules"]
+                    },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",

From 6249d764171d7dcb651ca9f4b4de85aacf855f21 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 8 Aug 2025 14:02:19 +0000
Subject: [PATCH 767/887] docs: updated changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index d56a8257b..273b8cf2f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -89,6 +89,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
 - Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
 - Modified `test_build` profile to use a reduced version of Pfam and Dfam files [#733](https://github.com/nf-core/rnafusion/pull/733)
+- Changed local `ARRIBA_VISUALIZATION`, `CTATSPLICING_STARTOCANCERINTRONS`, `CTATSPLICING_PREPGENOMELIB`, `FUSIONINSPECTOR`, `STARFUSION_DETECT` for its nf-core module versions [#XXX](https://github.com/nf-core/rnafusion/pull/XXX)
 
 ### Fixed
 

From cfac4cbaf9cf174579b0de5d99518faa318e77de Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 11 Aug 2025 11:06:56 +0200
Subject: [PATCH 768/887] fix output docs

---
 docs/output.md | 248 ++++++++++++++++++++++---------------------------
 1 file changed, 113 insertions(+), 135 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 24c6a2d8d..e8ad8fefd 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -11,8 +11,7 @@ The directories listed below will be created in the results directory after the
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
 - [Download and build references](#references) - Build references needed to run the rest of the pipeline
-- [STAR](#star) - Alignment for arriba, and STAR-fusion
-- [Cat](#cat) - Concatenate fastq files per sample ID
+- [STAR](#star) - Alignment of FASTQ files
 - [Arriba](#arriba) - Arriba fusion detection
 - [STAR-fusion](#starfusion) - STAR-fusion fusion detection
 - [StringTie](#stringtie) - StringTie assembly
@@ -35,28 +34,27 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 ### References directory structure
 
-- `references/`
-  - `arriba`
-    - `blacklist_hg38_GRCh38_v2.1.0.tsv.gz`
-    - `protein_domains_hg38_GRCh38_v2.1.0.gff3`
-    - `cytobands_hg38_GRCh38_v2.1.0.tsv`
-  - `ensembl`
-    - `Homo_sapiens.GRCh38.{ensembl_version}.all.fa`
-    - `Homo_sapiens.GRCh38.{ensembl_version}.cdna.all.fa.gz`
-    - `Homo_sapiens.GRCh38.{ensembl_version}.gtf`
-    - `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf`
-    - `Homo_sapiens.GRCh38.{ensembl_version}.chr.gtf.refflat`
-    - `Homo_sapiens.GRCh38.{ensembl_version}.interval_list`
-  - `fusioncatcher`
-    - `human_v<version>` - dir with all references for fusioncatcher
-  - `fusion_report_db`
-    - `cosmic.db`
-    - `fusiongdb2.db`
-    - `mitelman.db`
-  - `star` - dir with STAR index
-  - `starfusion`
-    - files and dirs used to build the index
-    - `ctat_genome_lib_build_dir` - dir containing the index
+- `<genomes_base>/`
+  - `<genome>/`
+    - `fusion_report_db`
+      - `cosmic.db`
+      - `fusiongdb2.db`
+      - `mitelman.db`
+    - `gencode_v<gencode_version>/`
+      - `gencode/`
+        - `Homo_sapiens.GRCh38.<gencode_version>.all.fa`
+        - `Homo_sapiens.GRCh38.<gencode_version>.cdna.all.fa.gz`
+        - `Homo_sapiens.GRCh38.<gencode_version>.gtf`
+        - `Homo_sapiens.GRCh38.<gencode_version>.chr.gtf`
+        - `Homo_sapiens.GRCh38.<gencode_version>.chr.gtf.refflat`
+        - `Homo_sapiens.GRCh38.<gencode_version>.interval_list`
+      - `salmon/`
+      - `star/`
+      - `starfusion/`
+        - `ctat_genome_lib_build_dir/`
+      - `hgnc/`
+        - `hgnc_complete_set.txt`
+        - `HGNC-DB-timestamp.txt`
 
 (Only files or folders used by the pipeline are mentioned explicitly.)
 
@@ -70,27 +68,43 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 ```text
 {outdir}
+├── agat
 ├── arriba
 ├── arriba_visualisation
+├── ctatsplicing
 ├── fastp
 ├── fastqc
+├── fastqc_for_fastp
 ├── fusioncatcher
 ├── fusioninspector
 ├── fusionreport
-├── kallisto_quant
-├── megafusion
+├── gatk4
+├── get
 ├── multiqc
 ├── picard
 ├── pipeline_info
-├── samtools_sort_for_arriba
+├── salmon
 ├── star
 ├── starfusion
-└── work
+├── stringtie
+└── vcf
 .nextflow.log
 ```
 
 If no parameters are specified, the default is applied.
 
+### Agat
+
+[Arriba](https://github.com/NBISweden/AGAT) is to convert the GFF file to a TSV file, which is then used in the VCF creation.
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `arriba/`
+  - `<sample>.tsv`
+
+</details>
+
 ### Arriba
 
 [Arriba](https://arriba.readthedocs.io/en/latest/) is used for i) detect gene fusions and ii) create a PDF report for the fusions found (visualisation):
@@ -118,22 +132,33 @@ If no parameters are specified, the default is applied.
 
 The visualisation displays the fusions that fusioninspector outputs. That means that fusions from all callers are aggregated (by fusion-report) and then analyzed through fusioninspector (Note: Fusioninspecor contains a filtering step!).
 
-### Cat
+### CTAT-SPLICING
+
+If `--tools ctatsplicing` is present, [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) will detect cancer splicing aberrations.
 
 <details markdown="1">
 <summary>Output files</summary>
 
-- `cat/`
-  - `<sample>_1.merged.fastq.gz`
-  - `<sample>_2.merged.fastq.gz`
+- `ctatsplicing/`
+  - `<sample>.cancer_intron_reads.sorted.bam`
+  - `<sample>.cancer_intron_reads.sorted.bam.bai`
+  - `<sample>.cancer.introns`
+  - `<sample>.cancer.introns.prelim`
+  - `<sample>.chckpts`
+  - `<sample>.ctat-splicing.igv.html`
+  - `<sample>.gene_reads.sorted.sifted.bam`
+  - `<sample>.gene_reads.sorted.sifted.bam.bai`
+  - `<sample>.igv.tracks`
+  - `<sample>.introns`
+  - `<sample>.introns.for_IGV.bed`
 
 </details>
 
-If multiple libraries or runs have been provided for the same sample in the input samplesheet (e.g. to increase sequencing depth) then these will be merged at the very beginning of the pipeline in order to have consistent sample naming throughout the pipeline. Please refer to the [usage](https://nf-co.re/rnafusion/usage#samplesheet-input) documentation to see how to specify these samples in the input samplesheet.
+[CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING/wiki) detects and annotates of aberrant splicing isoforms in cancer. This is run on the input files for `arriba` and/or `starfusion`.
 
 ### Fastp
 
-If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will filter low quality reads as well as bases at the 5' and 3' ends, trim adapters (automatically detected, but input with parameter `--adapter_fasta` is possible). 3' trimming is also possible via parameter `--trim_tail`.
+If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will filter low quality reads as well as bases at the 5' and 3' ends, trim adapters (automatically detected, but input with parameter `--adapter_fasta` is possible). 3' trimming is also possible via parameter `--trim_tail_fusioncatcher`.
 
 <details markdown="1">
 <summary>Output files</summary>
@@ -169,73 +194,39 @@ If `trim_tail_fusioncatcher` has any value other than 0, [fastp](https://github.
 <summary>Output files</summary>
 
 - `fastqc/`
-  - `*_fastqc.html`: FastQC report containing quality metrics.
-  - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
+  - `*.html`: FastQC report containing quality metrics.
+  - `*.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
 </details>
 
 [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
 
-### MultiQC
-
-![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png)
-
-![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png)
-
-![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png)
-
-:::note
-The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
-:::
-
-### FusionCatcher
+### FastQC for fastp
 
 <details markdown="1">
 <summary>Output files</summary>
 
-- `fusioncatcher`
-  - `<sample>.fusioncatcher.fusion-genes.txt`
-  - `<sample>.fusioncatcher.summary.txt`
-  - `<sample>.fusioncatcher.log`
+- `fastqc_for_fastp/`
+  - `*.html`: FastQC report containing quality metrics.
+  - `*.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
 </details>
 
-[FusionCatcher](https://github.com/ndaniel/fusioncatcher) searches for novel/known somatic fusion genes translocations, and chimeras in RNA-seq data. Possibility to use parameter `--fusioncatcher_limitSjdbInsertNsj` to modify limitSjdbInsertNsj.
+This directory contains FastQC reports for the fastp trimmed reads.
 
-### CTAT-SPLICING
+### FusionCatcher
 
 <details markdown="1">
 <summary>Output files</summary>
 
-- `ctatsplicing`
-  - `arriba`
-    - `<sample>.cancer_intron_reads.sorted.bam`
-    - `<sample>.cancer_intron_reads.sorted.bam.bai`
-    - `<sample>.cancer.introns`
-    - `<sample>.cancer.introns.prelim`
-    - `<sample>.chckpts`
-    - `<sample>.ctat-splicing.igv.html`
-    - `<sample>.gene_reads.sorted.sifted.bam`
-    - `<sample>.gene_reads.sorted.sifted.bam.bai`
-    - `<sample>.igv.tracks`
-    - `<sample>.introns`
-    - `<sample>.introns.for_IGV.bed`
-  - `starfusion`
-    - `<sample>.cancer_intron_reads.sorted.bam`
-    - `<sample>.cancer_intron_reads.sorted.bam.bai`
-    - `<sample>.cancer.introns`
-    - `<sample>.cancer.introns.prelim`
-    - `<sample>.chckpts`
-    - `<sample>.ctat-splicing.igv.html`
-    - `<sample>.gene_reads.sorted.sifted.bam`
-    - `<sample>.gene_reads.sorted.sifted.bam.bai`
-    - `<sample>.igv.tracks`
-    - `<sample>.introns`
-    - `<sample>.introns.for_IGV.bed`
+- `fusioncatcher`
+  - `<sample>.fusion-genes.txt`
+  - `<sample>.summary.txt`
+  - `<sample>.log`
 
 </details>
 
-[CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING/wiki) detects and annotates of aberrant splicing isoforms in cancer. This is run on the input files for `arriba` and/or `starfusion`.
+[FusionCatcher](https://github.com/ndaniel/fusioncatcher) searches for novel/known somatic fusion genes translocations, and chimeras in RNA-seq data. Possibility to use parameter `--fusioncatcher_limitSjdbInsertNsj` to modify limitSjdbInsertNsj.
 
 ### FusionInspector
 
@@ -243,9 +234,14 @@ The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They m
 <summary>Output files</summary>
 
 - `fusioninspector`
-  - `<sample>.fusion_inspector_web.html` - visualisation report described in details [here](https://github.com/FusionInspector/FusionInspector/wiki/FusionInspector-Visualizations)
-  - `FusionInspector.log`
-  - `<sample>.FusionInspector.fusions.abridged.tsv`
+  - `<sample>/`
+    - `chckpts_dir/` - contains checkpoints for FusionInspector
+    - `fi_workdir/` - The working directory for FusionInspector
+    - `FusionInspector.log`
+    - `IGV_inputs/` - contains IGV input files
+    - `<sample>.FusionInspector.fusions.abridged.tsv`
+    - `<sample>.FusionInspector.fusions.tsv`
+    - `<sample>.fusion_inspector_web.html` - visualisation report described in details [here](https://github.com/FusionInspector/FusionInspector/wiki/FusionInspector-Visualizations)
 
 </details>
 
@@ -260,10 +256,11 @@ Please note that fusion-report is executed from fork https://github.com/Clinical
 
 - `fusionreport`
   - <sample>
-    - `<sample>.fusionreport.tsv`
     - `<sample>.fusionreport_filtered.tsv`
     - `<sample>_fusionreport_index.html` - general report for all filtered fusions
+    - `<sample>.fusionreport.tsv`
     - `<sample>.fusions.csv` - index in csv format
+    - `<sample>.fusions.json` - index in json format
     - `<fusion>_<pair>.html` - specific report for each filtered fusion
 
 </details>
@@ -289,77 +286,59 @@ The Fusion Indication Index FII is calculated using two components:
 
 Final score = (0.5 × Tool Detection Score) + (0.5 × Database Hits Score)
 
-### Salmon
-
-<details markdown="1">
-<summary>Output files</summary>
-
-- `salmon`
-  - `<sample>`
-
-</details>
-
-Folder containing the quantification results
-
-### Kallisto
+### MultiQC
 
-<details markdown="1">
-<summary>Output files</summary>
+![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png)
 
-- `kallisto`
-  - `<sample>.kallisto_quant.fusions.txt`
+![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png)
 
-</details>
+![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png)
 
-Quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.
+:::note
+The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
+:::
 
-### Vcf_collect
+### Picard
 
 <details markdown="1">
 <summary>Output files</summary>
 
-- `vcf_collect`
-  - `<sample>_fusion_data.vcf` - contains the fusions in vcf format with collected statistics.
+Picard CollectRnaMetrics and picard MarkDuplicates share the same output directory.
 
-Vcf-collect takes as input the results of fusion-report and fusioninspector. That means fusions from all tools are aggregated. Fusioninspector applies a filter so it is possible some fusions detected by a caller are not filtered out by fusioninspector. In those cases, vcf-collect will display the fusions, but a lot of data will be missing as fusioninspector performs the analysis for each fusion.
+- `picard`
+  - `<sample>.MarkDuplicates.metrics.txt` - metrics from MarkDuplicates
+  - `<sample>_rna_metrics.txt` - metrics from CollectRnaMetrics
+  - `<sample>_insert_size_metrics.txt.txt` - metrics from CollectInsertSizeMetrics
+  - `<sample>.bam` - BAM file with marked duplicates
 
 </details>
 
-[Megafusion](https://github.com/J35P312/MegaFusion) converts RNA fusion files to SV VCF and collects statistics and metrics in a VCF file.
-
-### MultiQC
+### Pipeline information
 
 <details markdown="1">
 <summary>Output files</summary>
 
-- `multiqc/`
-  - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
-  - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
-  - `multiqc_plots/`: directory containing static images from the report in various formats.
+- `pipeline_info/`
+  - Reports generated by Nextflow: `execution_report_<timestamp>.html`, `execution_timeline_<timestamp>.html`, `execution_trace_<timestamp>.txt`, `pipeline_dag_<timestamp>.dot`/`pipeline_dag_<timestamp>.svg` and `manifest_<timestamp>.bco.json`.
+  - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
+  - Parameters used by the pipeline run: `params_<timestamp>.json`.
 
 </details>
 
-[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualized in the report and further statistics are available in the report data directory.
-
-Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
-
-### Pipeline information
+[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
 
-### Picard
+### Salmon
 
 <details markdown="1">
 <summary>Output files</summary>
 
-Picard CollectRnaMetrics and picard MarkDuplicates share the same output directory.
-
-- `picard`
-  - `<sample>.MarkDuplicates.metrics.txt` - metrics from MarkDuplicates
-  - `<sample>_rna_metrics.txt` - metrics from CollectRnaMetrics
-  - `<sample>_insert_size_metrics.txt.txt` - metrics from CollectInsertSizeMetrics
-  - `<sample>.bam` - BAM file with marked duplicates
+- `salmon`
+  - `<sample>`
 
 </details>
 
+Folder containing the quantification results
+
 ### STAR
 
 STAR is used to align FASTQ files to the genome reference.
@@ -406,17 +385,16 @@ The STAR index is generated with `--sjdbOverhang ${params.read_length - 1}`, `pa
 - `stringtie/<sample>/stringtie.merged.gtf` - merged gtf from annotation and stringtie output gtfs
 </details>
 
-### Pipeline information
+### Vcf_collect
 
 <details markdown="1">
 <summary>Output files</summary>
 
-- `pipeline_info/`
-  - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`.
-  - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline.
-  - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
-  - Parameters used by the pipeline run: `params.json`.
+- `vcf`
+  - `<sample>_fusion_data.vcf` - contains the fusions in vcf format with collected statistics.
+
+Vcf-collect takes as input the results of fusion-report and fusioninspector. That means fusions from all tools are aggregated. Fusioninspector applies a filter so it is possible some fusions detected by a caller are not filtered out by fusioninspector. In those cases, vcf-collect will display the fusions, but a lot of data will be missing as fusioninspector performs the analysis for each fusion.
 
 </details>
 
-[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
+[Megafusion](https://github.com/J35P312/MegaFusion) converts RNA fusion files to SV VCF and collects statistics and metrics in a VCF file.

From acfb48bd656bc94e605c48f1ea42f56aa104e16a Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 11 Aug 2025 11:08:36 +0200
Subject: [PATCH 769/887] changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c586d3f46..1a35d7776 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -89,6 +89,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
 - Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
 - Modified `test_build` profile to use a reduced version of Pfam and Dfam files [#733](https://github.com/nf-core/rnafusion/pull/733)
+- Updated the documentation to reflect the changes done in the release [#741](https://github.com/nf-core/rnafusion/pull/741)
 
 ### Fixed
 

From 332a20c090f18c219e9108974b105d5491ebab42 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Wed, 13 Aug 2025 11:35:17 -0300
Subject: [PATCH 770/887] Add test for `vcf_collect` module

---
 modules/local/vcf_collect/tests/main.nf.test | 56 ++++++++++++++++++++
 1 file changed, 56 insertions(+)
 create mode 100644 modules/local/vcf_collect/tests/main.nf.test

diff --git a/modules/local/vcf_collect/tests/main.nf.test b/modules/local/vcf_collect/tests/main.nf.test
new file mode 100644
index 000000000..559849301
--- /dev/null
+++ b/modules/local/vcf_collect/tests/main.nf.test
@@ -0,0 +1,56 @@
+nextflow_process {
+
+    name "Test Process VCF_COLLECT"
+    script "../main.nf"
+    process "VCF_COLLECT"
+
+    test("VCF_COLLECT - human - fusionreport - fusioninspector") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([ id:'test_sample' ])
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/test.FusionInspector.fusions.abridged.tsv", checkIfExists: true)))
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/test.tsv", checkIfExists: true)))
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/fusionreport/test_sample_fusionreport_index.html", checkIfExists: true)))
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/test.fusions.csv", checkIfExists: true)))
+                input[1] = [[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")]
+                input[2] = [[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("VCF_COLLECT - human - fusionreport - fusioninspector - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([ id:'test_sample' ])
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/test.FusionInspector.fusions.abridged.tsv", checkIfExists: true)))
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/test.tsv", checkIfExists: true)))
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/fusionreport/test_sample_fusionreport_index.html", checkIfExists: true)))
+                    .combine(Channel.of(file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/human/test.fusions.csv", checkIfExists: true)))
+                input[1] = [[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")]
+                input[2] = [[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}

From 86cb5c5729bf8cbbc6ef91470d8db02d606d5ae2 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Wed, 13 Aug 2025 11:36:13 -0300
Subject: [PATCH 771/887] Add snapshot

---
 .../local/vcf_collect/tests/main.nf.test.snap | 68 +++++++++++++++++++
 1 file changed, 68 insertions(+)
 create mode 100644 modules/local/vcf_collect/tests/main.nf.test.snap

diff --git a/modules/local/vcf_collect/tests/main.nf.test.snap b/modules/local/vcf_collect/tests/main.nf.test.snap
new file mode 100644
index 000000000..1e203d0c1
--- /dev/null
+++ b/modules/local/vcf_collect/tests/main.nf.test.snap
@@ -0,0 +1,68 @@
+{
+    "VCF_COLLECT - human - fusionreport - fusioninspector - stub": {
+        "content": [
+            {
+                "0": [
+                    "versions.yml:md5,0e9daf1986d2c5f0ce94eb1ca2cb4fed"
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_fusion_data.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_fusion_data.vcf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,0e9daf1986d2c5f0ce94eb1ca2cb4fed"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-12T20:45:40.474082615"
+    },
+    "VCF_COLLECT - human - fusionreport - fusioninspector": {
+        "content": [
+            {
+                "0": [
+                    "versions.yml:md5,646edd06d506d0b35d32ee663bafb804"
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_fusion_data.vcf:md5,0ae5bd8c5b24ffbec7ae617feec91583"
+                    ]
+                ],
+                "vcf": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_fusion_data.vcf:md5,0ae5bd8c5b24ffbec7ae617feec91583"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,646edd06d506d0b35d32ee663bafb804"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-12T20:45:25.283350308"
+    }
+}

From 074ea3f986a7bcd931349ef4c2bf2c033b0d926a Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Wed, 13 Aug 2025 11:44:32 -0300
Subject: [PATCH 772/887] Update CHANGELOG.md

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c586d3f46..429e40475 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -48,6 +48,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added a extra trimming step for fusioncatcher in case a different read-length is wished for this tool only [#674](https://github.com/nf-core/rnafusion/pull/674)
 - Added support for references hosted on the nf-core AWS S3 bucket. [#717](https://github.com/nf-core/rnafusion/pull/717)
 - Added `--dfam_hmm`, `--dfam_h3f`, `--dfam_h3i`, `--dfam_h3m`, `--dfam_h3p`, `--pfam_file` and `--annot_filter_url` parameters to allow use of custom files in `STARFUSION_BUILD` module [#709](https://github.com/nf-core/rnafusion/pull/709)
+- Add nf-test to local module: `VCF_COLLECT`. [#745](https://github.com/nf-core/rnafusion/pull/745)
 
 ### Changed
 

From c3d7044ba75d17378f3dd40590f48f66ba8ac847 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 18 Aug 2025 17:06:39 +0000
Subject: [PATCH 773/887] test: update snapshot

---
 tests/test_build.nf.test.snap    |   4 +-
 tests/test_stub.nf.test.snap     | 256 +++----------------------------
 tests/test_stub_bam.nf.test.snap | 256 +++----------------------------
 3 files changed, 38 insertions(+), 478 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index ee462d5d6..74ceef667 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -10,7 +10,7 @@
                     "bioawk": 1.0
                 },
                 "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
+                    "ctatsplicing": "0.0.2"
                 },
                 "FUSIONREPORT_DOWNLOAD": {
                     "fusion_report": "4.0.1"
@@ -285,6 +285,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:11:40.714960564"
+        "timestamp": "2025-08-18T13:58:42.038319834"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index a8212ba9d..759d060d9 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -40,14 +40,14 @@
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
                     "STAR-Fusion": "1.15.1"
                 },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -218,7 +218,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:20:09.057111889"
+        "timestamp": "2025-08-18T16:11:25.285702343"
     },
     "stub test stringtie": {
         "content": [
@@ -528,7 +528,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
+                    "FusionInspector": "2.10.0"
                 },
                 "FUSIONREPORT_DETECT": {
                     "fusion_report": "4.0.1"
@@ -608,119 +608,9 @@
                 "fusioninspector/test",
                 "fusioninspector/test/FusionInspector.log",
                 "fusioninspector/test/IGV_inputs",
-                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/test/IGV_inputs/cytoBand.txt",
-                "fusioninspector/test/IGV_inputs/test.ROI.bed",
-                "fusioninspector/test/IGV_inputs/test.bed",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.fa",
-                "fusioninspector/test/IGV_inputs/test.fa.fai",
-                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/test/IGV_inputs/test.gtf",
-                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/tracks.json",
                 "fusioninspector/test/chckpts_dir",
-                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/test/chckpts_dir/blast_filter.ok",
-                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/test/chckpts_dir/cp_final.ok",
-                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/test/chckpts_dir/cytoband.ok",
-                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/test/chckpts_dir/final.abridged.ok",
-                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/test/chckpts_dir/microH.dat.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/test/chckpts_dir/run_STAR.ok",
-                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
                 "fusioninspector/test/fi_workdir",
-                "fusioninspector/test/fi_workdir/Log.final.out",
-                "fusioninspector/test/fi_workdir/Log.out",
-                "fusioninspector/test/fi_workdir/Log.progress.out",
-                "fusioninspector/test/fi_workdir/SJ.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/test/fi_workdir/microH.dat",
-                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/test/fi_workdir/star_align.ok",
-                "fusioninspector/test/fi_workdir/test.fa",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
                 "fusioninspector/test/fi_workdir/test.gtf",
-                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
                 "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test/test.fusion_inspector_web.html",
@@ -887,7 +777,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:23:44.5776928"
+        "timestamp": "2025-08-18T16:16:11.496999226"
     },
     "stub test salmon": {
         "content": [
@@ -995,7 +885,7 @@
                     "bioawk": 1.0
                 },
                 "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
+                    "ctatsplicing": "0.0.2"
                 },
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
@@ -1219,7 +1109,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:17:54.284398814"
+        "timestamp": "2025-08-18T16:08:54.904059765"
     },
     "stub test fusionreport": {
         "content": [
@@ -1274,14 +1164,14 @@
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
                     "STAR-Fusion": "1.15.1"
                 },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -1473,7 +1363,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:27:41.801614257"
+        "timestamp": "2025-08-18T16:13:31.815555556"
     },
     "stub test fastp": {
         "content": [
@@ -1728,7 +1618,7 @@
                     "bioawk": 1.0
                 },
                 "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
+                    "ctatsplicing": "0.0.2"
                 },
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
@@ -1746,7 +1636,7 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
+                    "FusionInspector": "2.10.0"
                 },
                 "FUSIONREPORT_DETECT": {
                     "fusion_report": "4.0.1"
@@ -1794,14 +1684,14 @@
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
                     "STAR-Fusion": "1.15.1"
                 },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -1869,119 +1759,9 @@
                 "fusioninspector/test",
                 "fusioninspector/test/FusionInspector.log",
                 "fusioninspector/test/IGV_inputs",
-                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/test/IGV_inputs/cytoBand.txt",
-                "fusioninspector/test/IGV_inputs/test.ROI.bed",
-                "fusioninspector/test/IGV_inputs/test.bed",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.fa",
-                "fusioninspector/test/IGV_inputs/test.fa.fai",
-                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/test/IGV_inputs/test.gtf",
-                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/tracks.json",
                 "fusioninspector/test/chckpts_dir",
-                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/test/chckpts_dir/blast_filter.ok",
-                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/test/chckpts_dir/cp_final.ok",
-                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/test/chckpts_dir/cytoband.ok",
-                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/test/chckpts_dir/final.abridged.ok",
-                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/test/chckpts_dir/microH.dat.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/test/chckpts_dir/run_STAR.ok",
-                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
                 "fusioninspector/test/fi_workdir",
-                "fusioninspector/test/fi_workdir/Log.final.out",
-                "fusioninspector/test/fi_workdir/Log.out",
-                "fusioninspector/test/fi_workdir/Log.progress.out",
-                "fusioninspector/test/fi_workdir/SJ.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/test/fi_workdir/microH.dat",
-                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/test/fi_workdir/star_align.ok",
-                "fusioninspector/test/fi_workdir/test.fa",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
                 "fusioninspector/test/fi_workdir/test.gtf",
-                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
                 "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test/test.fusion_inspector_web.html",
@@ -2185,6 +1965,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:15:49.690844884"
+        "timestamp": "2025-08-18T16:02:59.744224309"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 439a583a4..762751f28 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -37,14 +37,14 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
                     "STAR-Fusion": "1.15.1"
                 },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -191,7 +191,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:34:21.840598583"
+        "timestamp": "2025-08-18T15:54:01.219397324"
     },
     "stub test stringtie": {
         "content": [
@@ -450,7 +450,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
+                    "FusionInspector": "2.10.0"
                 },
                 "FUSIONREPORT_DETECT": {
                     "fusion_report": "4.0.1"
@@ -522,119 +522,9 @@
                 "fusioninspector/test",
                 "fusioninspector/test/FusionInspector.log",
                 "fusioninspector/test/IGV_inputs",
-                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/test/IGV_inputs/cytoBand.txt",
-                "fusioninspector/test/IGV_inputs/test.ROI.bed",
-                "fusioninspector/test/IGV_inputs/test.bed",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.fa",
-                "fusioninspector/test/IGV_inputs/test.fa.fai",
-                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/test/IGV_inputs/test.gtf",
-                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/tracks.json",
                 "fusioninspector/test/chckpts_dir",
-                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/test/chckpts_dir/blast_filter.ok",
-                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/test/chckpts_dir/cp_final.ok",
-                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/test/chckpts_dir/cytoband.ok",
-                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/test/chckpts_dir/final.abridged.ok",
-                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/test/chckpts_dir/microH.dat.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/test/chckpts_dir/run_STAR.ok",
-                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
                 "fusioninspector/test/fi_workdir",
-                "fusioninspector/test/fi_workdir/Log.final.out",
-                "fusioninspector/test/fi_workdir/Log.out",
-                "fusioninspector/test/fi_workdir/Log.progress.out",
-                "fusioninspector/test/fi_workdir/SJ.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/test/fi_workdir/microH.dat",
-                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/test/fi_workdir/star_align.ok",
-                "fusioninspector/test/fi_workdir/test.fa",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
                 "fusioninspector/test/fi_workdir/test.gtf",
-                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
                 "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test/test.fusion_inspector_web.html",
@@ -785,7 +675,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:38:28.107838971"
+        "timestamp": "2025-08-18T16:00:15.182860428"
     },
     "stub test salmon": {
         "content": [
@@ -912,7 +802,7 @@
                     "bioawk": 1.0
                 },
                 "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
+                    "ctatsplicing": "0.0.2"
                 },
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
@@ -1112,7 +1002,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:32:37.072437849"
+        "timestamp": "2025-08-18T15:47:14.034109802"
     },
     "stub test fusionreport": {
         "content": [
@@ -1164,14 +1054,14 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
                     "STAR-Fusion": "1.15.1"
                 },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -1339,7 +1229,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:36:12.01932932"
+        "timestamp": "2025-08-18T15:56:39.559031879"
     },
     "stub test fastp": {
         "content": [
@@ -1551,7 +1441,7 @@
                     "bioawk": 1.0
                 },
                 "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
+                    "ctatsplicing": "0.0.2"
                 },
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
@@ -1569,7 +1459,7 @@
                     "fusioncatcher": "1.33"
                 },
                 "FUSIONINSPECTOR": {
-                    "STAR-Fusion": null
+                    "FusionInspector": "2.10.0"
                 },
                 "FUSIONREPORT_DETECT": {
                     "fusion_report": "4.0.1"
@@ -1614,14 +1504,14 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
-                "STARFUSION": {
-                    "STAR-Fusion": "1.15.0"
-                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
                     "STAR-Fusion": "1.15.1"
                 },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
                 "STAR_GENOMEGENERATE": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -1684,119 +1574,9 @@
                 "fusioninspector/test",
                 "fusioninspector/test/FusionInspector.log",
                 "fusioninspector/test/IGV_inputs",
-                "fusioninspector/test/IGV_inputs/TrinityFusion.bed",
-                "fusioninspector/test/IGV_inputs/cytoBand.txt",
-                "fusioninspector/test/IGV_inputs/test.ROI.bed",
-                "fusioninspector/test/IGV_inputs/test.bed",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz",
-                "fusioninspector/test/IGV_inputs/test.bed.sorted.bed.gz.tbi",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam",
-                "fusioninspector/test/IGV_inputs/test.consolidated.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.fa",
-                "fusioninspector/test/IGV_inputs/test.fa.fai",
-                "fusioninspector/test/IGV_inputs/test.fusion_inspector_web.json",
-                "fusioninspector/test/IGV_inputs/test.gtf",
-                "fusioninspector/test/IGV_inputs/test.igv.Pfam.bed",
-                "fusioninspector/test/IGV_inputs/test.igv.seqsimilar.bed",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.junction_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam",
-                "fusioninspector/test/IGV_inputs/test.spanning_reads.bam.bai",
-                "fusioninspector/test/IGV_inputs/tracks.json",
                 "fusioninspector/test/chckpts_dir",
-                "fusioninspector/test/chckpts_dir/EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/add_FFPM.ok",
-                "fusioninspector/test/chckpts_dir/add_splice_info.ok",
-                "fusioninspector/test/chckpts_dir/append_microH_info.ok",
-                "fusioninspector/test/chckpts_dir/blast_filter.ok",
-                "fusioninspector/test/chckpts_dir/coalesce_junc_n_span.ok",
-                "fusioninspector/test/chckpts_dir/cp_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/cp_contigs_file_workdir",
-                "fusioninspector/test/chckpts_dir/cp_final.ok",
-                "fusioninspector/test/chckpts_dir/cp_gtf_file_workdir.ok",
-                "fusioninspector/test/chckpts_dir/cp_tracks_json.ok",
-                "fusioninspector/test/chckpts_dir/create_fi_igvjs.ok",
-                "fusioninspector/test/chckpts_dir/cytoband.ok",
-                "fusioninspector/test/chckpts_dir/filter_by_frag_threshs.ok",
-                "fusioninspector/test/chckpts_dir/final.abridged.ok",
-                "fusioninspector/test/chckpts_dir/fusion_annotator.ok",
-                "fusioninspector/test/chckpts_dir/fusion_coding_region_effect.ok",
-                "fusioninspector/test/chckpts_dir/fusion_contigs.ok",
-                "fusioninspector/test/chckpts_dir/fusion_reports_html.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok",
-                "fusioninspector/test/chckpts_dir/index_consol_bam.ok",
-                "fusioninspector/test/chckpts_dir/init_EM_adj_counts.ok",
-                "fusioninspector/test/chckpts_dir/init_spanning_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/mark_dup_reads.ok",
-                "fusioninspector/test/chckpts_dir/mark_dups_reads.index.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_fai.ok",
-                "fusioninspector/test/chckpts_dir/merged_contig_gtf_to_bed.ok",
-                "fusioninspector/test/chckpts_dir/microH.dat.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_extract_junc_reads.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_pfam_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_bed.ok",
-                "fusioninspector/test/chckpts_dir/prep_igv_seqsim_gff3.ok",
-                "fusioninspector/test/chckpts_dir/prep_spanning_reads.ok",
-                "fusioninspector/test/chckpts_dir/run_STAR.ok",
-                "fusioninspector/test/chckpts_dir/samtools_idx_junc_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/samtools_index_span_reads_bam.ok",
-                "fusioninspector/test/chckpts_dir/span_reads_acc.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bedsort.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.bgzip.ok",
-                "fusioninspector/test/chckpts_dir/test.bed.tabix.ok",
                 "fusioninspector/test/fi_workdir",
-                "fusioninspector/test/fi_workdir/Log.final.out",
-                "fusioninspector/test/fi_workdir/Log.out",
-                "fusioninspector/test/fi_workdir/Log.progress.out",
-                "fusioninspector/test/fi_workdir/SJ.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonGeTrInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/exonInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/geneInfo.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbInfo.txt",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.fromGTF.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/sjdbList.out.tab",
-                "fusioninspector/test/fi_workdir/_STARgenome/transcriptInfo.tab",
-                "fusioninspector/test/fi_workdir/microH.dat",
-                "fusioninspector/test/fi_workdir/pipeliner.456.cmds",
-                "fusioninspector/test/fi_workdir/star_align.ok",
-                "fusioninspector/test/fi_workdir/test.fa",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter.info",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_junction_read_accs",
-                "fusioninspector/test/fi_workdir/test.fusion_preds.coalesced.summary.fusion_spanning_read_accs",
                 "fusioninspector/test/fi_workdir/test.gtf",
-                "fusioninspector/test/fi_workdir/test.igv.Pfam.gff3",
-                "fusioninspector/test/fi_workdir/test.igv.seqsimilar.gff3",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated",
-                "fusioninspector/test/fi_workdir/test.post_blast_and_promiscuity_filter.EMadj.FFPM.wMicroH.annotated.coding_effect",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.failed_reads_during_span_analysis",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_junction_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_span_reads.sam",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.fusion_spanning_info",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.read_align_counts.idx.ok",
-                "fusioninspector/test/fi_workdir/test.star.cSorted.dupsMarked.bam.spanning_reads_want.idx",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.bai.ok",
-                "fusioninspector/test/fi_workdir/test.star.sortedByCoord.out.bam.ok",
                 "fusioninspector/test/test.FusionInspector.fusions.abridged.tsv",
                 "fusioninspector/test/test.FusionInspector.fusions.tsv",
                 "fusioninspector/test/test.fusion_inspector_web.html",
@@ -1984,6 +1764,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:30:49.983961991"
+        "timestamp": "2025-08-18T15:39:59.605183405"
     }
 }
\ No newline at end of file

From 75a2cdd3874cddc987b71eeda4d1813c160c1be9 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 18 Aug 2025 17:07:15 +0000
Subject: [PATCH 774/887] fix: make changes to avoid module failure

---
 .../ctatsplicing/prepgenomelib/main.nf        | 82 +------------------
 1 file changed, 3 insertions(+), 79 deletions(-)

diff --git a/modules/nf-core/ctatsplicing/prepgenomelib/main.nf b/modules/nf-core/ctatsplicing/prepgenomelib/main.nf
index 3a3539822..c87dc8907 100644
--- a/modules/nf-core/ctatsplicing/prepgenomelib/main.nf
+++ b/modules/nf-core/ctatsplicing/prepgenomelib/main.nf
@@ -1,7 +1,6 @@
 process CTATSPLICING_PREPGENOMELIB {
     tag "$meta.id"
     label 'process_single'
-    stageInMode 'copy'
 
     container "nf-core/ctatsplicing:0.0.3"
 
@@ -10,7 +9,7 @@ process CTATSPLICING_PREPGENOMELIB {
     path(cancer_intron_tsv)
 
     output:
-    tuple val(meta), path(genome_lib), emit: reference
+    tuple val(meta), path(genome_lib, includeInputs:true), emit: reference
     path "versions.yml"              , emit: versions
 
     when:
@@ -40,85 +39,10 @@ process CTATSPLICING_PREPGENOMELIB {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     """
-    mkdir -p $genome_lib/
-    mkdir -p $genome_lib/ref_genome.fa.star.idx
-    mkdir -p $genome_lib/__chkpts
-    mkdir -p $genome_lib/cancer_splicing_lib
-    touch $genome_lib/ref_genome.fa.star.idx/chrName.txt
-    touch $genome_lib/ref_genome.fa.star.idx/exonInfo.tab
-    touch $genome_lib/ref_genome.fa.star.idx/SAindex
-    touch $genome_lib/ref_genome.fa.star.idx/Genome
-    touch $genome_lib/ref_genome.fa.star.idx/geneInfo.tab
-    touch $genome_lib/ref_genome.fa.star.idx/sjdbList.out.tab
-    touch $genome_lib/ref_genome.fa.star.idx/sjdbInfo.txt
-    touch $genome_lib/ref_genome.fa.star.idx/transcriptInfo.tab
-    touch $genome_lib/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab
-    touch $genome_lib/ref_genome.fa.star.idx/build.ok
-    touch $genome_lib/ref_genome.fa.star.idx/chrLength.txt
-    touch $genome_lib/ref_genome.fa.star.idx/Log.out
-    touch $genome_lib/ref_genome.fa.star.idx/genomeParameters.txt
-    touch $genome_lib/ref_genome.fa.star.idx/chrStart.txt
-    touch $genome_lib/ref_genome.fa.star.idx/SA
-    touch $genome_lib/ref_genome.fa.star.idx/chrNameLength.txt
-    touch $genome_lib/ref_genome.fa.star.idx/exonGeTrInfo.tab
-    touch $genome_lib/ref_annot.cds
-    touch $genome_lib/ref_annot.gtf.gene_spans
-    touch $genome_lib/pfam_domains.dbm
-    touch $genome_lib/ref_annot.cdsplus.fa.idx
-    touch $genome_lib/ref_genome.fa.ndb
-    touch $genome_lib/ref_genome.fa.nin
-    touch $genome_lib/ref_genome.fa
-    touch $genome_lib/ref_annot.prot_info.dbm
-    echo | gzip > $genome_lib/PFAM.domtblout.dat.gz
-    touch $genome_lib/trans.blast.align_coords.align_coords.dat
-    touch $genome_lib/fusion_annot_lib.idx
-    touch $genome_lib/ref_annot.pep
-    touch $genome_lib/__chkpts/trans.blast.dat.cp.ok
-    touch $genome_lib/__chkpts/cp_ref_annot_cdna.ok
-    touch $genome_lib/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok
-    touch $genome_lib/__chkpts/annotfiltrule_cp.ok
-    touch $genome_lib/__chkpts/validate_ctat_genome_lib.ok
-    touch $genome_lib/__chkpts/cp_pfam_dat.ok
-    touch $genome_lib/__chkpts/fusion_annot_lib.cp.ok
-    touch $genome_lib/__chkpts/ref_genome.fa.ok
-    touch $genome_lib/__chkpts/cp_gene_blast_pairs.ok
-    touch $genome_lib/__chkpts/mm2_genome_idx.ok
-    touch $genome_lib/__chkpts/trans.blast.dat.index.ok
-    touch $genome_lib/__chkpts/ref_genome_fai.ok
-    touch $genome_lib/__chkpts/index_ref_annot_cdna.ok
-    touch $genome_lib/__chkpts/ref_annot.gtf.ok
-    touch $genome_lib/__chkpts/blast_pairs.idx.ok
-    touch $genome_lib/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok
-    touch $genome_lib/__chkpts/_prot_info_db.ok
-    touch $genome_lib/__chkpts/_fusion_annot_lib.idx.ok
-    touch $genome_lib/__chkpts/index_pfam_hits.ok
-    touch $genome_lib/__chkpts/makeblastdb.ok
-    touch $genome_lib/__chkpts/ref_annot.gtf.gene_spans.ok
-    touch $genome_lib/__chkpts/mm2.splice_bed.ok
-    touch $genome_lib/__chkpts/ref_annot.gtf.mini.sortu.ok
-    touch $genome_lib/ref_genome.fa.ntf
-    touch $genome_lib/ref_genome.fa.nto
-    touch $genome_lib/ref_annot.gtf
-    touch $genome_lib/ref_annot.gtf.mm2.splice.bed
-    touch $genome_lib/ref_genome.fa.mm2
-    echo | gzip > $genome_lib/fusion_annot_lib.gz
-    touch $genome_lib/ref_annot.cdsplus.fa
-    touch $genome_lib/AnnotFilterRule.pm
-    echo | gzip > $genome_lib/trans.blast.dat.gz
-    touch $genome_lib/ref_genome.fa.nhr
-    touch $genome_lib/blast_pairs.idx
-    touch $genome_lib/ref_genome.fa.nsq
-    echo | gzip > $genome_lib/blast_pairs.dat.gz
-    touch $genome_lib/ref_annot.cdna.fa.idx
-    touch $genome_lib/ref_genome.fa.not
-    touch $genome_lib/ref_annot.cdna.fa
-    touch $genome_lib/ref_annot.gtf.mini.sortu
-    touch $genome_lib/trans.blast.align_coords.align_coords.dbm
-    touch $genome_lib/ref_genome.fa.njs
-    touch $genome_lib/ref_genome.fa.fai
     touch $genome_lib/refGene.bed
     echo | gzip > $genome_lib/refGene.sort.bed.gz
-    echo | gzip > $genome_lib/refGene.sort.bed.gz.tbi
+    touch $genome_lib/refGene.sort.bed.gz.tbi
+    mkdir $genome_lib/cancer_splicing_lib
     touch $genome_lib/cancer_splicing_lib/cancer_splicing.idx
 
     cat <<-END_VERSIONS > versions.yml

From f7be12e5db2934b89b180bb9e68ce41d504cf497 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Mon, 18 Aug 2025 17:26:06 +0000
Subject: [PATCH 775/887] fix: patch module temporarily until changes are made
 in nf-core

---
 modules.json                                  |   6 +-
 .../ctatsplicing-prepgenomelib.diff           | 114 +++++++++++++++++
 .../ctatsplicing-startocancerintrons.diff     | 120 ++++++++++++++++++
 3 files changed, 238 insertions(+), 2 deletions(-)
 create mode 100644 modules/nf-core/ctatsplicing/prepgenomelib/ctatsplicing-prepgenomelib.diff
 create mode 100644 modules/nf-core/ctatsplicing/startocancerintrons/ctatsplicing-startocancerintrons.diff

diff --git a/modules.json b/modules.json
index a4cd21862..31bc8b665 100644
--- a/modules.json
+++ b/modules.json
@@ -43,12 +43,14 @@
                     "ctatsplicing/prepgenomelib": {
                         "branch": "master",
                         "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/ctatsplicing/prepgenomelib/ctatsplicing-prepgenomelib.diff"
                     },
                     "ctatsplicing/startocancerintrons": {
                         "branch": "master",
                         "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
-                        "installed_by": ["modules"]
+                        "installed_by": ["modules"],
+                        "patch": "modules/nf-core/ctatsplicing/startocancerintrons/ctatsplicing-startocancerintrons.diff"
                     },
                     "fastp": {
                         "branch": "master",
diff --git a/modules/nf-core/ctatsplicing/prepgenomelib/ctatsplicing-prepgenomelib.diff b/modules/nf-core/ctatsplicing/prepgenomelib/ctatsplicing-prepgenomelib.diff
new file mode 100644
index 000000000..cfb1ff5f4
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/prepgenomelib/ctatsplicing-prepgenomelib.diff
@@ -0,0 +1,114 @@
+Changes in component 'nf-core/ctatsplicing/prepgenomelib'
+'modules/nf-core/ctatsplicing/prepgenomelib/meta.yml' is unchanged
+Changes in 'ctatsplicing/prepgenomelib/main.nf':
+--- modules/nf-core/ctatsplicing/prepgenomelib/main.nf
++++ modules/nf-core/ctatsplicing/prepgenomelib/main.nf
+@@ -1,7 +1,6 @@
+ process CTATSPLICING_PREPGENOMELIB {
+     tag "$meta.id"
+     label 'process_single'
+-    stageInMode 'copy'
+ 
+     container "nf-core/ctatsplicing:0.0.3"
+ 
+@@ -10,7 +9,7 @@
+     path(cancer_intron_tsv)
+ 
+     output:
+-    tuple val(meta), path(genome_lib), emit: reference
++    tuple val(meta), path(genome_lib, includeInputs:true), emit: reference
+     path "versions.yml"              , emit: versions
+ 
+     when:
+@@ -40,85 +39,10 @@
+     def prefix = task.ext.prefix ?: "${meta.id}"
+     def VERSION = '0.0.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+     """
+-    mkdir -p $genome_lib/
+-    mkdir -p $genome_lib/ref_genome.fa.star.idx
+-    mkdir -p $genome_lib/__chkpts
+-    mkdir -p $genome_lib/cancer_splicing_lib
+-    touch $genome_lib/ref_genome.fa.star.idx/chrName.txt
+-    touch $genome_lib/ref_genome.fa.star.idx/exonInfo.tab
+-    touch $genome_lib/ref_genome.fa.star.idx/SAindex
+-    touch $genome_lib/ref_genome.fa.star.idx/Genome
+-    touch $genome_lib/ref_genome.fa.star.idx/geneInfo.tab
+-    touch $genome_lib/ref_genome.fa.star.idx/sjdbList.out.tab
+-    touch $genome_lib/ref_genome.fa.star.idx/sjdbInfo.txt
+-    touch $genome_lib/ref_genome.fa.star.idx/transcriptInfo.tab
+-    touch $genome_lib/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab
+-    touch $genome_lib/ref_genome.fa.star.idx/build.ok
+-    touch $genome_lib/ref_genome.fa.star.idx/chrLength.txt
+-    touch $genome_lib/ref_genome.fa.star.idx/Log.out
+-    touch $genome_lib/ref_genome.fa.star.idx/genomeParameters.txt
+-    touch $genome_lib/ref_genome.fa.star.idx/chrStart.txt
+-    touch $genome_lib/ref_genome.fa.star.idx/SA
+-    touch $genome_lib/ref_genome.fa.star.idx/chrNameLength.txt
+-    touch $genome_lib/ref_genome.fa.star.idx/exonGeTrInfo.tab
+-    touch $genome_lib/ref_annot.cds
+-    touch $genome_lib/ref_annot.gtf.gene_spans
+-    touch $genome_lib/pfam_domains.dbm
+-    touch $genome_lib/ref_annot.cdsplus.fa.idx
+-    touch $genome_lib/ref_genome.fa.ndb
+-    touch $genome_lib/ref_genome.fa.nin
+-    touch $genome_lib/ref_genome.fa
+-    touch $genome_lib/ref_annot.prot_info.dbm
+-    echo | gzip > $genome_lib/PFAM.domtblout.dat.gz
+-    touch $genome_lib/trans.blast.align_coords.align_coords.dat
+-    touch $genome_lib/fusion_annot_lib.idx
+-    touch $genome_lib/ref_annot.pep
+-    touch $genome_lib/__chkpts/trans.blast.dat.cp.ok
+-    touch $genome_lib/__chkpts/cp_ref_annot_cdna.ok
+-    touch $genome_lib/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok
+-    touch $genome_lib/__chkpts/annotfiltrule_cp.ok
+-    touch $genome_lib/__chkpts/validate_ctat_genome_lib.ok
+-    touch $genome_lib/__chkpts/cp_pfam_dat.ok
+-    touch $genome_lib/__chkpts/fusion_annot_lib.cp.ok
+-    touch $genome_lib/__chkpts/ref_genome.fa.ok
+-    touch $genome_lib/__chkpts/cp_gene_blast_pairs.ok
+-    touch $genome_lib/__chkpts/mm2_genome_idx.ok
+-    touch $genome_lib/__chkpts/trans.blast.dat.index.ok
+-    touch $genome_lib/__chkpts/ref_genome_fai.ok
+-    touch $genome_lib/__chkpts/index_ref_annot_cdna.ok
+-    touch $genome_lib/__chkpts/ref_annot.gtf.ok
+-    touch $genome_lib/__chkpts/blast_pairs.idx.ok
+-    touch $genome_lib/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok
+-    touch $genome_lib/__chkpts/_prot_info_db.ok
+-    touch $genome_lib/__chkpts/_fusion_annot_lib.idx.ok
+-    touch $genome_lib/__chkpts/index_pfam_hits.ok
+-    touch $genome_lib/__chkpts/makeblastdb.ok
+-    touch $genome_lib/__chkpts/ref_annot.gtf.gene_spans.ok
+-    touch $genome_lib/__chkpts/mm2.splice_bed.ok
+-    touch $genome_lib/__chkpts/ref_annot.gtf.mini.sortu.ok
+-    touch $genome_lib/ref_genome.fa.ntf
+-    touch $genome_lib/ref_genome.fa.nto
+-    touch $genome_lib/ref_annot.gtf
+-    touch $genome_lib/ref_annot.gtf.mm2.splice.bed
+-    touch $genome_lib/ref_genome.fa.mm2
+-    echo | gzip > $genome_lib/fusion_annot_lib.gz
+-    touch $genome_lib/ref_annot.cdsplus.fa
+-    touch $genome_lib/AnnotFilterRule.pm
+-    echo | gzip > $genome_lib/trans.blast.dat.gz
+-    touch $genome_lib/ref_genome.fa.nhr
+-    touch $genome_lib/blast_pairs.idx
+-    touch $genome_lib/ref_genome.fa.nsq
+-    echo | gzip > $genome_lib/blast_pairs.dat.gz
+-    touch $genome_lib/ref_annot.cdna.fa.idx
+-    touch $genome_lib/ref_genome.fa.not
+-    touch $genome_lib/ref_annot.cdna.fa
+-    touch $genome_lib/ref_annot.gtf.mini.sortu
+-    touch $genome_lib/trans.blast.align_coords.align_coords.dbm
+-    touch $genome_lib/ref_genome.fa.njs
+-    touch $genome_lib/ref_genome.fa.fai
+     touch $genome_lib/refGene.bed
+     echo | gzip > $genome_lib/refGene.sort.bed.gz
+-    echo | gzip > $genome_lib/refGene.sort.bed.gz.tbi
++    touch $genome_lib/refGene.sort.bed.gz.tbi
++    mkdir $genome_lib/cancer_splicing_lib
+     touch $genome_lib/cancer_splicing_lib/cancer_splicing.idx
+ 
+     cat <<-END_VERSIONS > versions.yml
+
+'modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test' is unchanged
+'modules/nf-core/ctatsplicing/prepgenomelib/tests/main.nf.test.snap' is unchanged
+************************************************************
diff --git a/modules/nf-core/ctatsplicing/startocancerintrons/ctatsplicing-startocancerintrons.diff b/modules/nf-core/ctatsplicing/startocancerintrons/ctatsplicing-startocancerintrons.diff
new file mode 100644
index 000000000..7ec847711
--- /dev/null
+++ b/modules/nf-core/ctatsplicing/startocancerintrons/ctatsplicing-startocancerintrons.diff
@@ -0,0 +1,120 @@
+Changes in component 'nf-core/ctatsplicing/startocancerintrons'
+'modules/nf-core/ctatsplicing/startocancerintrons/main.nf' is unchanged
+Changes in 'ctatsplicing/startocancerintrons/meta.yml':
+--- modules/nf-core/ctatsplicing/startocancerintrons/meta.yml
++++ modules/nf-core/ctatsplicing/startocancerintrons/meta.yml
+@@ -63,7 +63,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.cancer_intron_reads.sorted.bam':
++      - "*.cancer_intron_reads.sorted.bam":
+           type: file
+           description: "Sorted BAM file containing reads mapped to cancer introns"
+           pattern: "*.cancer_intron_reads.sorted.bam"
+@@ -75,7 +75,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.cancer_intron_reads.sorted.bam.bai':
++      - "*.cancer_intron_reads.sorted.bam.bai":
+           type: file
+           description: "Index file for the sorted BAM of cancer introns"
+           pattern: "*.cancer_intron_reads.sorted.bam.bai"
+@@ -87,7 +87,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.gene_reads.sorted.sifted.bam':
++      - "*.gene_reads.sorted.sifted.bam":
+           type: file
+           description: "Sorted BAM file containing reads mapped to gene regions after downsampling coverage"
+           pattern: "*.gene_reads.sorted.sifted.bam"
+@@ -99,7 +99,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.gene_reads.sorted.sifted.bam.bai':
++      - "*.gene_reads.sorted.sifted.bam.bai":
+           type: file
+           description: "Index file for the sorted BAM of gene reads after filtering"
+           pattern: "*.gene_reads.sorted.sifted.bam.bai"
+@@ -111,7 +111,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.cancer.introns':
++      - "*.cancer.introns":
+           type: file
+           description: "File containing detected and filtered introns that are found to be enriched in cancer transcriptomes"
+           pattern: "*.cancer.introns"
+@@ -121,7 +121,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.cancer.introns.prelim':
++      - "*.cancer.introns.prelim":
+           type: file
+           description: "File containing detected introns that have at least one supporting read and are found to be enriched in cancer transcriptomes"
+           pattern: "*.cancer.introns.prelim"
+@@ -131,7 +131,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*${prefix}.introns':
++      - "*${prefix}.introns":
+           type: file
+           description: "File containing all detected introns"
+           pattern: "*${prefix}.introns"
+@@ -141,7 +141,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.introns.for_IGV.bed':
++      - "*.introns.for_IGV.bed":
+           type: file
+           description: "Bed file used as input for IGV-report visualization, containing cancer introns"
+           pattern: "*.introns.for_IGV.bed"
+@@ -153,7 +153,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.ctat-splicing.igv.html':
++      - "*.ctat-splicing.igv.html":
+           type: file
+           description: "Self-contained IGV-report in HTML format for visualization of cancer introns"
+           pattern: "*.ctat-splicing.igv.html"
+@@ -163,7 +163,7 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.igv.tracks':
++      - "*.igv.tracks":
+           type: file
+           description: "IGV tracks file for visualizing cancer introns in IGV"
+           pattern: "*.igv.tracks"
+@@ -173,15 +173,15 @@
+           description: |
+             Groovy Map containing sample information
+             e.g. [ id:'test', single_end:false ]
+-      - '*.chckpts':
++      - "*.chckpts":
+           type: file
+           description: "Checkpoint files for CTAT-SPLICING, useful for debugging or resuming interrupted runs"
+           pattern: "*.chckpts"
+   versions:
+     - versions.yml:
+-          type: file
+-          description: File containing software versions
+-          pattern: "versions.yml"
++        type: file
++        description: File containing software versions
++        pattern: "versions.yml"
+ authors:
+   - "@anoronh4"
+   - "@nvnieuwk"
+
+'modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test' is unchanged
+'modules/nf-core/ctatsplicing/startocancerintrons/tests/main.nf.test.snap' is unchanged
+'modules/nf-core/ctatsplicing/startocancerintrons/tests/nextflow.config' is unchanged
+************************************************************

From ade0511f7fe43375e8738d6c06d5d217e2394439 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 20 Aug 2025 17:08:39 +0200
Subject: [PATCH 776/887] update starfusion detect

---
 CHANGELOG.md                              | 2 +-
 modules.json                              | 2 +-
 modules/nf-core/starfusion/detect/main.nf | 4 +++-
 3 files changed, 5 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 266fe7ceb..12ffb5127 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -90,7 +90,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
 - Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
 - Modified `test_build` profile to use a reduced version of Pfam and Dfam files [#733](https://github.com/nf-core/rnafusion/pull/733)
-- Changed local `ARRIBA_VISUALIZATION`, `CTATSPLICING_STARTOCANCERINTRONS`, `CTATSPLICING_PREPGENOMELIB`, `FUSIONINSPECTOR`, `STARFUSION_DETECT` for its nf-core module versions [#XXX](https://github.com/nf-core/rnafusion/pull/XXX)
+- Changed local `ARRIBA_VISUALIZATION`, `CTATSPLICING_STARTOCANCERINTRONS`, `CTATSPLICING_PREPGENOMELIB`, `FUSIONINSPECTOR`, `STARFUSION_DETECT` for its nf-core module versions [#740](https://github.com/nf-core/rnafusion/pull/740)
 
 ### Fixed
 
diff --git a/modules.json b/modules.json
index 31bc8b665..61e76f0a3 100644
--- a/modules.json
+++ b/modules.json
@@ -169,7 +169,7 @@
                     },
                     "starfusion/detect": {
                         "branch": "master",
-                        "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
+                        "git_sha": "b0f38d0ea7515484442edc96046858924fc477dc",
                         "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
diff --git a/modules/nf-core/starfusion/detect/main.nf b/modules/nf-core/starfusion/detect/main.nf
index 5211a3100..acec416a4 100644
--- a/modules/nf-core/starfusion/detect/main.nf
+++ b/modules/nf-core/starfusion/detect/main.nf
@@ -37,7 +37,9 @@ process STARFUSION_DETECT {
 
     mv star-fusion.fusion_predictions.tsv ${prefix}.fusion_predictions.tsv
     mv star-fusion.fusion_predictions.abridged.tsv ${prefix}.abridged.tsv
-    mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${prefix}.abridged.coding_effect.tsv
+    if [ -f star-fusion.fusion_predictions.abridged.coding_effect.tsv ]; then
+        mv star-fusion.fusion_predictions.abridged.coding_effect.tsv ${prefix}.abridged.coding_effect.tsv
+    fi
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From 5747e6e50f165043aaac76249bfa495f5876df95 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 20 Aug 2025 19:10:15 +0000
Subject: [PATCH 777/887] fix: detect `transcript_type` in gtf

---
 conf/modules.config           | 3 +--
 conf/test_build.config        | 3 ++-
 tests/test_build.nf.test.snap | 8 ++++----
 3 files changed, 7 insertions(+), 7 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index ef6741548..8e0ba6060 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -43,8 +43,7 @@ process {
             saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
         ]
         ext.prefix =  { "${meta.id}_rrna.gtf" }
-        // Should we also have \$9 ~ /transcript_type "rRNA"/ as part of the filtering? See 's3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf' structure.
-        ext.args   = """-c gff '\$9 ~ /transcript_biotype "rRNA"/ || \$9 ~ /transcript_biotype "Mt_rRNA"/ || \$9 ~ /transcript_biotype "rRNA_pseudogene"/'"""
+        ext.args   = """-c gff '\$9 ~ /transcript_biotype "rRNA"/ || \$9 ~ /transcript_type "rRNA"/ || \$9 ~ /transcript_type "Mt_rRNA"/ || \$9 ~ /transcript_type "rRNA_pseudogene"/ || \$9 ~ /transcript_biotype "Mt_rRNA"/ || \$9 ~ /transcript_biotype "rRNA_pseudogene"/'"""
     }
 
     withName: 'CTATSPLICING_STARTOCANCERINTRONS' {
diff --git a/conf/test_build.config b/conf/test_build.config
index 8fbb75ba5..c1a7a9251 100644
--- a/conf/test_build.config
+++ b/conf/test_build.config
@@ -13,7 +13,8 @@ This test is designed to test the `build_references` subworkflow with the follow
 - SAMTOOLS_FAIDX
 - HGNC_DOWNLOAD
 - GATK4_CREATESEQUENCEDICTIONARY
-- GET_RRNA_TRANSCRIPTS
+- BIOAWK
+- AGAT_CONVERTGFF2BED
 - GATK4_BEDTOINTERVALLIST
 - GTF_TO_REFFLAT
 - GFFREAD
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 74ceef667..689fb5f67 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -191,13 +191,13 @@
                 "references/GRCh38/hgnc/hgnc_complete_set.txt"
             ],
             [
-                "minigenome.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "minigenome.gtf_rrna.gtf.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "minigenome.gtf.bed:md5,4c881266e0a1b1a41815d05a8055aa40",
+                "minigenome.gtf_rrna.gtf.gz:md5,fb03ec66f68bc4becc71c299ed38b62d",
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
-                "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
+                "minigenome.gtf.interval_list:md5,6496748a0994cb8a9764761ae74f8004",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
@@ -285,6 +285,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T13:58:42.038319834"
+        "timestamp": "2025-08-20T19:03:37.62529837"
     }
 }
\ No newline at end of file

From b3cd9a9b63ccd4859fc5f65313e9327b1aba0fce Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Wed, 20 Aug 2025 19:12:20 +0000
Subject: [PATCH 778/887] docs: update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 266fe7ceb..ff8b2e3ba 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -114,6 +114,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix CTAT-SPLICING output when no cancer introns were found [#722](https://github.com/nf-core/rnafusion/pull/722)
 - Update VCF_COLLECT script to adapt to transcript_version not being an entry in fusioninspector gtf anymore [#726](https://github.com/nf-core/rnafusion/pull/726)
 - Fix rRNA detection and make it more customizable with nf-core modules [#736](https://github.com/nf-core/rnafusion/pull/736)
+- Fix rRNA detection in GTF using `transcript_type` [#749](https://github.com/nf-core/rnafusion/pull/749)
 
 ### Removed
 

From 342d4fa64c94e912032acd93d5dedd43f1b82031 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 21 Aug 2025 11:27:43 +0200
Subject: [PATCH 779/887] add arriba fusion back to fusioninspector input

---
 workflows/rnafusion.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index beccf1c93..06a5dd26c 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -240,7 +240,7 @@ workflow RNAFUSION {
                     BUILD_REFERENCES.out.arriba_ref_cytobands,
                     BUILD_REFERENCES.out.arriba_ref_protein_domains
                 )
-
+                ch_arriba_fusions = ARRIBA_ARRIBA.out.fusions
                 ch_versions = ch_versions.mix(ARRIBA_ARRIBA.out.versions)
             }
         }

From 006b5cd844e1de1b99368b1176c9468952eda108 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Thu, 21 Aug 2025 12:50:05 +0200
Subject: [PATCH 780/887] fix null params issues

---
 subworkflows/local/build_references/main.nf | 10 ++++++++++
 1 file changed, 10 insertions(+)

diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index d71369070..a7e9e859a 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -234,7 +234,17 @@ workflow BUILD_REFERENCES {
 ========================================================================================
 */
 
+//
+// A function to test if a file exists and is not empty.
+//   Input: A string that represents a file path
+//   Output: A boolean
+//
 def exists_not_empty(path) {
+    // Return false for invalid values
+    if(!path) {
+        return false
+    }
+
     def path_to_check = file(path as String)
     // Return false if the path does not exist
     if(!path_to_check.exists()) {

From 19887f6b87d492d5e08be46bd833d0d7d468ce1f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Thu, 21 Aug 2025 15:02:57 +0200
Subject: [PATCH 781/887] Update docs/usage.md

Co-authored-by: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index 8d3ca0d3f..d05e4f6f9 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -145,7 +145,7 @@ TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,forward
 | `sample`           | Custom sample name. This value needs to be unique across all entries in the samplesheet and cannot contain spaces                                                                                                                                                                                                                                                                                                                             | ✅       |
 | `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                                                                                                             | ✅       |
 | `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.                                                                                      | ❓       |
-| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq. It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.gz".                                                                                      | ❓       |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`".                                                                                      | ❓       |
 | `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.   | ❓       |
 | `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                                                                                                                 | ❌       |
 | `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty. | ❓       |

From 5a38668aff4440ea787f92f7d2632f23f9e0c832 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 21 Aug 2025 19:26:01 +0000
Subject: [PATCH 782/887] refact: replace local `trim_workflow` for nf-core
 subworkflow with same functionality

---
 .vscode/settings.json                         |    3 +-
 modules.json                                  |   14 +-
 .../nf-core/umitools/extract/environment.yml  |    7 +
 modules/nf-core/umitools/extract/main.nf      |   74 +
 modules/nf-core/umitools/extract/meta.yml     |   64 +
 .../umitools/extract/tests/main.nf.test       |  106 +
 .../umitools/extract/tests/main.nf.test.snap  |  167 ++
 .../umitools/extract/tests/nextflow.config    |    9 +
 subworkflows/local/trim_workflow/main.nf      |   40 -
 .../local/trim_workflow/tests/main.nf.test    |  110 -
 .../trim_workflow/tests/main.nf.test.snap     |  138 -
 .../fastq_fastqc_umitools_fastp/main.nf       |  153 ++
 .../fastq_fastqc_umitools_fastp/meta.yml      |  129 +
 .../tests/main.nf.test                        |  996 +++++++
 .../tests/main.nf.test.snap                   | 2407 +++++++++++++++++
 .../tests/nextflow.config                     |   16 +
 tests/test_build.nf.test.snap                 |    2 +-
 tests/test_stub.nf.test.snap                  |   54 +-
 tests/test_stub_bam.nf.test                   |    1 -
 tests/test_stub_bam.nf.test.snap              |   50 +-
 workflows/rnafusion.nf                        |   68 +-
 21 files changed, 4229 insertions(+), 379 deletions(-)
 create mode 100644 modules/nf-core/umitools/extract/environment.yml
 create mode 100644 modules/nf-core/umitools/extract/main.nf
 create mode 100644 modules/nf-core/umitools/extract/meta.yml
 create mode 100644 modules/nf-core/umitools/extract/tests/main.nf.test
 create mode 100644 modules/nf-core/umitools/extract/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/umitools/extract/tests/nextflow.config
 delete mode 100644 subworkflows/local/trim_workflow/main.nf
 delete mode 100644 subworkflows/local/trim_workflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/trim_workflow/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config

diff --git a/.vscode/settings.json b/.vscode/settings.json
index a33b527cc..af2d783fa 100644
--- a/.vscode/settings.json
+++ b/.vscode/settings.json
@@ -1,3 +1,4 @@
 {
-    "markdown.styles": ["public/vscode_markdown.css"]
+    "markdown.styles": ["public/vscode_markdown.css"],
+    "nextflow.telemetry.enabled": false
 }
diff --git a/modules.json b/modules.json
index 61e76f0a3..c7c9e6d86 100644
--- a/modules.json
+++ b/modules.json
@@ -55,12 +55,12 @@
                     "fastp": {
                         "branch": "master",
                         "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
-                        "installed_by": ["modules"]
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
@@ -186,11 +186,21 @@
                         "branch": "master",
                         "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
                         "installed_by": ["modules"]
+                    },
+                    "umitools/extract": {
+                        "branch": "master",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "installed_by": ["fastq_fastqc_umitools_fastp"]
                     }
                 }
             },
             "subworkflows": {
                 "nf-core": {
+                    "fastq_fastqc_umitools_fastp": {
+                        "branch": "master",
+                        "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
+                        "installed_by": ["subworkflows"]
+                    },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
diff --git a/modules/nf-core/umitools/extract/environment.yml b/modules/nf-core/umitools/extract/environment.yml
new file mode 100644
index 000000000..e5721f1e7
--- /dev/null
+++ b/modules/nf-core/umitools/extract/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::umi_tools=1.1.5
diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf
new file mode 100644
index 000000000..b97900e09
--- /dev/null
+++ b/modules/nf-core/umitools/extract/main.nf
@@ -0,0 +1,74 @@
+process UMITOOLS_EXTRACT {
+    tag "$meta.id"
+    label "process_single"
+    label "process_long"
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/umi_tools:1.1.5--py39hf95cd2a_0' :
+        'biocontainers/umi_tools:1.1.5--py39hf95cd2a_0' }"
+
+    input:
+    tuple val(meta), path(reads)
+
+    output:
+    tuple val(meta), path("*.fastq.gz"), emit: reads
+    tuple val(meta), path("*.log")     , emit: log
+    path  "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if (meta.single_end) {
+        """
+        umi_tools \\
+            extract \\
+            -I $reads \\
+            -S ${prefix}.umi_extract.fastq.gz \\
+            $args \\
+            > ${prefix}.umi_extract.log
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
+        END_VERSIONS
+        """
+    }  else {
+        """
+        umi_tools \\
+            extract \\
+            -I ${reads[0]} \\
+            --read2-in=${reads[1]} \\
+            -S ${prefix}.umi_extract_1.fastq.gz \\
+            --read2-out=${prefix}.umi_extract_2.fastq.gz \\
+            $args \\
+            > ${prefix}.umi_extract.log
+
+        cat <<-END_VERSIONS > versions.yml
+        "${task.process}":
+            umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
+        END_VERSIONS
+        """
+    }
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    if (meta.single_end) {
+        output_command = "echo '' | gzip > ${prefix}.umi_extract.fastq.gz"
+    } else {
+        output_command = "echo '' | gzip > ${prefix}.umi_extract_1.fastq.gz ;"
+        output_command += "echo '' | gzip > ${prefix}.umi_extract_2.fastq.gz"
+    }
+    """
+    touch ${prefix}.umi_extract.log
+    ${output_command}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' )
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/umitools/extract/meta.yml b/modules/nf-core/umitools/extract/meta.yml
new file mode 100644
index 000000000..19a7fa644
--- /dev/null
+++ b/modules/nf-core/umitools/extract/meta.yml
@@ -0,0 +1,64 @@
+name: umitools_extract
+description: Extracts UMI barcode from a read and add it to the read name, leaving
+  any sample barcode in place
+keywords:
+  - UMI
+  - barcode
+  - extract
+  - umitools
+tools:
+  - umi_tools:
+      description: >
+        UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random
+        Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes
+      documentation: https://umi-tools.readthedocs.io/en/latest/
+      license: "MIT"
+      identifier: ""
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - reads:
+        type: list
+        description: |
+          List of input FASTQ files whose UMIs will be extracted.
+output:
+  reads:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.fastq.gz":
+          type: file
+          description: >
+            Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz.
+            | For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
+          pattern: "*.{fastq.gz}"
+          ontologies: []
+  log:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.log":
+          type: file
+          description: Logfile for umi_tools
+          pattern: "*.{log}"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@drpatelh"
+  - "@grst"
+maintainers:
+  - "@drpatelh"
+  - "@grst"
diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test b/modules/nf-core/umitools/extract/tests/main.nf.test
new file mode 100644
index 000000000..bb8a06582
--- /dev/null
+++ b/modules/nf-core/umitools/extract/tests/main.nf.test
@@ -0,0 +1,106 @@
+nextflow_process {
+
+    name "Test Process UMITOOLS_EXTRACT"
+    script "../main.nf"
+    process "UMITOOLS_EXTRACT"
+    config "./nextflow.config"
+    tag "modules_nfcore"
+    tag "modules"
+    tag "umitools"
+    tag "umitools/extract"
+
+    test("single end") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    process.out.reads.collect { it.collect { it instanceof Map ? it : file(it).name }},
+                    process.out.log.collect { it.collect { it instanceof Map ? it : file(it).name }},
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("single end - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("pair end") {
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                    file(process.out.reads[0][1][0]).name,
+                    file(process.out.reads[0][1][1]).name,
+                    process.out.log.collect { it.collect { it instanceof Map ? it : file(it).name }},
+                    process.out.versions
+                ).match() }
+            )
+        }
+    }
+
+    test("pair end - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = [ [ id:'test', single_end:false ], // meta map
+                    [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
+                    file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true) ]
+                ]
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test.snap b/modules/nf-core/umitools/extract/tests/main.nf.test.snap
new file mode 100644
index 000000000..b1159054b
--- /dev/null
+++ b/modules/nf-core/umitools/extract/tests/main.nf.test.snap
@@ -0,0 +1,167 @@
+{
+    "pair end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.umi_extract_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.umi_extract_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.umi_extract.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,568d243174c081a0301e74ed42e59b48"
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.umi_extract.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test.umi_extract_1.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test.umi_extract_2.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,568d243174c081a0301e74ed42e59b48"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-02T15:05:20.008312"
+    },
+    "single end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.umi_extract.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.umi_extract.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    "versions.yml:md5,568d243174c081a0301e74ed42e59b48"
+                ],
+                "log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.umi_extract.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.umi_extract.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,568d243174c081a0301e74ed42e59b48"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-02T15:04:12.145999"
+    },
+    "pair end": {
+        "content": [
+            "test.umi_extract_1.fastq.gz",
+            "test.umi_extract_2.fastq.gz",
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.umi_extract.log"
+                ]
+            ],
+            [
+                "versions.yml:md5,568d243174c081a0301e74ed42e59b48"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-02T15:21:09.578031"
+    },
+    "single end": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.umi_extract.fastq.gz"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.umi_extract.log"
+                ]
+            ],
+            [
+                "versions.yml:md5,568d243174c081a0301e74ed42e59b48"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "24.04.2"
+        },
+        "timestamp": "2024-07-02T15:03:52.464606"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/umitools/extract/tests/nextflow.config b/modules/nf-core/umitools/extract/tests/nextflow.config
new file mode 100644
index 000000000..c866f5a00
--- /dev/null
+++ b/modules/nf-core/umitools/extract/tests/nextflow.config
@@ -0,0 +1,9 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+    
+    withName: UMITOOLS_EXTRACT {
+        ext.args = '--bc-pattern="NNNN"'
+    }
+
+}
diff --git a/subworkflows/local/trim_workflow/main.nf b/subworkflows/local/trim_workflow/main.nf
deleted file mode 100644
index ec7b6199e..000000000
--- a/subworkflows/local/trim_workflow/main.nf
+++ /dev/null
@@ -1,40 +0,0 @@
-
-
-include { FASTP                      } from '../../../modules/nf-core/fastp/main'
-include { FASTQC as FASTQC_FOR_FASTP } from '../../../modules/nf-core/fastqc/main'
-
-workflow TRIM_WORKFLOW {
-
-    take:
-        reads           // channel [ meta, [ fastq files ] ]
-        adapter_fasta   // channel [ path ]
-        skip_qc         // boolean
-
-    main:
-        ch_versions       = Channel.empty()
-        ch_fastp_html     = Channel.empty()
-        ch_fastp_json     = Channel.empty()
-        ch_fastqc_trimmed = Channel.empty()
-
-        FASTP(reads, adapter_fasta, false, false, false)
-        ch_versions = ch_versions.mix(FASTP.out.versions)
-
-        if(!skip_qc) {
-            FASTQC_FOR_FASTP(FASTP.out.reads)
-            ch_versions       = ch_versions.mix(FASTQC_FOR_FASTP.out.versions)
-            ch_fastqc_trimmed = FASTQC_FOR_FASTP.out.zip
-        }
-
-        ch_reads_all           = FASTP.out.reads
-        ch_reads_fusioncatcher = ch_reads_all
-        ch_fastp_html          = FASTP.out.html
-        ch_fastp_json          = FASTP.out.json
-
-    emit:
-        ch_reads_all            // Channel [ meta, [reads]   ]
-        ch_reads_fusioncatcher  // Channel [ meta, [reads]   ]
-        ch_fastp_html           // Channel [ meta, path_html ]
-        ch_fastp_json           // Channel [ meta, path_json ]
-        ch_fastqc_trimmed       // Channel [ meta, path_zip  ]
-        versions = ch_versions  // Channel [ versions        ]
-    }
diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test b/subworkflows/local/trim_workflow/tests/main.nf.test
deleted file mode 100644
index 3151ebb2b..000000000
--- a/subworkflows/local/trim_workflow/tests/main.nf.test
+++ /dev/null
@@ -1,110 +0,0 @@
-nextflow_workflow {
-
-    name "Test Subworkflow QC_WORKFLOW"
-    script "../main.nf"
-    workflow "TRIM_WORKFLOW"
-    tag "qc"
-    tag "subworkflow"
-    tag "fastqc"
-    tag "fastp"
-
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true") {
-
-        when {
-
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [id: "test_fastq"],
-                        [
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
-                        ]
-                    ])
-                input[1] = Channel.empty()
-                input[2] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(
-                    workflow.out.ch_reads_all,
-                    workflow.out.ch_fastp_json,
-                    workflow.out.versions
-                ).match() },
-            )
-        }
-    }
-
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true + fastp_adaptors") {
-
-        when {
-
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [id: "test_fastq"],
-                        [
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
-                        ]
-                    ])
-                input[1] = Channel.fromPath('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/delete_me/fastp/adapters.fasta', checkIfExists: true)
-                input[2] = true
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(
-                        workflow.out.ch_reads_all,
-                        workflow.out.ch_fastp_json,
-                        workflow.out.versions
-                    ).match() }
-            )
-        }
-    }
-
-    test("TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp skip_qc== false") {
-
-        when {
-
-            workflow {
-                """
-                // ch_reads
-                input[0] = Channel.of(
-                    [
-                        [id: "test_fastq"],
-                        [
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz", checkIfExists: true),
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz", checkIfExists: true)
-                        ]
-                    ])
-                input[1] = Channel.empty()
-                input[2] = false
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(
-                        workflow.out.ch_reads_all,
-                        workflow.out.ch_fastp_json,
-                        workflow.out.versions
-                    ).match() }
-            )
-        }
-    }
-
-}
diff --git a/subworkflows/local/trim_workflow/tests/main.nf.test.snap b/subworkflows/local/trim_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 829c57265..000000000
--- a/subworkflows/local/trim_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,138 +0,0 @@
-{
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true + fastp_adaptors": {
-        "content": [
-            [
-                [
-                    {
-                        "id": "test_fastq"
-                    },
-                    [
-                        "test_fastq_1.fastp.fastq.gz:md5,adc67a7b4d0bf3520866d7599a4ba814",
-                        "test_fastq_2.fastp.fastq.gz:md5,9ee7d6c5230442970997477464255e67"
-                    ]
-                ]
-            ],
-            [
-                [
-                    {
-                        "id": "test_fastq"
-                    },
-                    "test_fastq.fastp.json:md5,38711d953a615ef8d88edceaa518d2ac"
-                ]
-            ],
-            [
-                "versions.yml:md5,bc4adfa947f4bf5a3558bcf09213841c"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-14T17:07:52.648611"
-    },
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true": {
-        "content": [
-            [
-                "versions.yml:md5,16187796d989b6260f572247e7dc0fc6",
-                "versions.yml:md5,ea42abe9875f41f8362a55ee7533f102"
-            ],
-            [
-                "test_fastq_1.fastp.fastq.gz:md5,0c436583301dea48755a5252a2675b64",
-                "test_fastq_2.fastp.fastq.gz:md5,f7f38138255e63b33286b819b6177612"
-            ],
-            [
-                "test_fastq_1.fastp.fastq.gz:md5,0c436583301dea48755a5252a2675b64",
-                "test_fastq_2.fastp.fastq.gz:md5,f7f38138255e63b33286b819b6177612"
-            ],
-            "test_fastq.fastp.html",
-            "test_fastq.fastp.json:md5,62066ad48c3d5981045cdd43e354cb2b",
-            [
-                "test_fastq_trimmed_1_fastqc.zip",
-                "test_fastq_trimmed_2_fastqc.zip"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-05T16:21:52.926289296"
-    },
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp skip_qc== false": {
-        "content": [
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-14T16:43:42.01598"
-    },
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp - skip_qc == true": {
-        "content": [
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-14T17:02:48.598962"
-    },
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == false": {
-        "content": [
-            [
-                
-            ],
-            [
-                
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-14T16:22:58.668715"
-    },
-    "TRIM_WORKFLOW - Homo sapiens - FASTQs - fastp == true + fasp_adaptors": {
-        "content": [
-            [
-                "versions.yml:md5,16187796d989b6260f572247e7dc0fc6",
-                "versions.yml:md5,ea42abe9875f41f8362a55ee7533f102"
-            ],
-            [
-                "test_fastq_1.fastp.fastq.gz:md5,adc67a7b4d0bf3520866d7599a4ba814",
-                "test_fastq_2.fastp.fastq.gz:md5,9ee7d6c5230442970997477464255e67"
-            ],
-            [
-                "test_fastq_1.fastp.fastq.gz:md5,adc67a7b4d0bf3520866d7599a4ba814",
-                "test_fastq_2.fastp.fastq.gz:md5,9ee7d6c5230442970997477464255e67"
-            ],
-            "test_fastq.fastp.html",
-            "test_fastq.fastp.json:md5,feb3483311bfa4ded60146f1cbc13fd5",
-            [
-                "test_fastq_trimmed_1_fastqc.zip",
-                "test_fastq_trimmed_2_fastqc.zip"
-            ]
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-05T16:22:26.29488483"
-    }
-}
\ No newline at end of file
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
new file mode 100644
index 000000000..170ce78c3
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf
@@ -0,0 +1,153 @@
+//
+// Read QC, UMI extraction and trimming
+//
+include { FASTQC as FASTQC_RAW  } from '../../../modules/nf-core/fastqc/main'
+include { FASTQC as FASTQC_TRIM } from '../../../modules/nf-core/fastqc/main'
+include { UMITOOLS_EXTRACT      } from '../../../modules/nf-core/umitools/extract/main'
+include { FASTP                 } from '../../../modules/nf-core/fastp/main'
+
+//
+// Function that parses fastp json output file to get total number of reads after trimming
+//
+
+def getFastpReadsAfterFiltering(json_file, min_num_reads) {
+
+    if (workflow.stubRun) {
+        return min_num_reads
+    }
+
+    def json = new groovy.json.JsonSlurper().parseText(json_file.text).get('summary') as Map
+    return json['after_filtering']['total_reads'].toLong()
+}
+
+def getFastpAdapterSequence(json_file) {
+    // Handle stub runs
+    if (workflow.stubRun) {
+        return ""
+    }
+
+    def json = new groovy.json.JsonSlurper().parseText(json_file.text) as Map
+    try {
+        return json['adapter_cutting']['read1_adapter_sequence']
+    }
+    catch (Exception ex) {
+        return ""
+    }
+}
+
+workflow FASTQ_FASTQC_UMITOOLS_FASTP {
+    take:
+    reads             // channel: [ val(meta), [ reads ] ]
+    skip_fastqc       // boolean: true/false
+    with_umi          // boolean: true/false
+    skip_umi_extract  // boolean: true/false
+    umi_discard_read  // integer: 0, 1 or 2
+    skip_trimming     // boolean: true/false
+    adapter_fasta     // file: adapter.fasta
+    save_trimmed_fail // boolean: true/false
+    save_merged       // boolean: true/false
+    min_trimmed_reads // integer: > 0
+
+    main:
+    ch_versions = Channel.empty()
+    fastqc_raw_html = Channel.empty()
+    fastqc_raw_zip = Channel.empty()
+    umi_log = Channel.empty()
+    trim_json = Channel.empty()
+    trim_html = Channel.empty()
+    trim_log = Channel.empty()
+    trim_reads_fail = Channel.empty()
+    trim_reads_merged = Channel.empty()
+    fastqc_trim_html = Channel.empty()
+    fastqc_trim_zip = Channel.empty()
+    trim_read_count = Channel.empty()
+    adapter_seq = Channel.empty()
+
+    if (!skip_fastqc) {
+        FASTQC_RAW(
+            reads
+        )
+        fastqc_raw_html = FASTQC_RAW.out.html
+        fastqc_raw_zip = FASTQC_RAW.out.zip
+        ch_versions = ch_versions.mix(FASTQC_RAW.out.versions.first())
+    }
+
+    umi_reads = reads
+    if (with_umi && !skip_umi_extract) {
+        UMITOOLS_EXTRACT(
+            reads
+        )
+        umi_reads = UMITOOLS_EXTRACT.out.reads
+        umi_log = UMITOOLS_EXTRACT.out.log
+        ch_versions = ch_versions.mix(UMITOOLS_EXTRACT.out.versions.first())
+
+        // Discard R1 / R2 if required
+        if (umi_discard_read in [1, 2]) {
+            UMITOOLS_EXTRACT.out.reads
+                .map { meta, _reads ->
+                    meta.single_end ? [meta, _reads] : [meta + [single_end: true], _reads[umi_discard_read % 2]]
+                }
+                .set { umi_reads }
+        }
+    }
+
+    trim_reads = umi_reads
+    if (!skip_trimming) {
+        FASTP(
+            umi_reads,
+            adapter_fasta,
+            false,
+            save_trimmed_fail,
+            save_merged,
+        )
+        trim_json = FASTP.out.json
+        trim_html = FASTP.out.html
+        trim_log = FASTP.out.log
+        trim_reads_fail = FASTP.out.reads_fail
+        trim_reads_merged = FASTP.out.reads_merged
+        ch_versions = ch_versions.mix(FASTP.out.versions.first())
+
+        //
+        // Filter FastQ files based on minimum trimmed read count after adapter trimming
+        //
+        FASTP.out.reads.join(trim_json).map { meta, _reads, json -> [meta, _reads, getFastpReadsAfterFiltering(json, min_trimmed_reads.toLong())] }.set { ch_num_trimmed_reads }
+
+        ch_num_trimmed_reads
+            .filter { _meta, _reads, num_reads -> num_reads >= min_trimmed_reads.toLong() }
+            .map { meta, _reads, _num_reads -> [meta, _reads] }
+            .set { trim_reads }
+
+        ch_num_trimmed_reads
+            .map { meta, _reads, num_reads -> [meta, num_reads] }
+            .set { trim_read_count }
+
+        trim_json
+            .map { meta, json -> [meta, getFastpAdapterSequence(json)] }
+            .set { adapter_seq }
+
+        if (!skip_fastqc) {
+            FASTQC_TRIM(
+                trim_reads
+            )
+            fastqc_trim_html = FASTQC_TRIM.out.html
+            fastqc_trim_zip = FASTQC_TRIM.out.zip
+            ch_versions = ch_versions.mix(FASTQC_TRIM.out.versions.first())
+        }
+    }
+
+    emit:
+    reads             = trim_reads // channel: [ val(meta), [ reads ] ]
+    fastqc_raw_html   // channel: [ val(meta), [ html ] ]
+    fastqc_raw_zip    // channel: [ val(meta), [ zip ] ]
+    umi_log           // channel: [ val(meta), [ log ] ]
+    adapter_seq       // channel: [ val(meta), [ adapter_seq] ]
+    trim_json         // channel: [ val(meta), [ json ] ]
+    trim_html         // channel: [ val(meta), [ html ] ]
+    trim_log          // channel: [ val(meta), [ log ] ]
+    trim_reads_fail   // channel: [ val(meta), [ fastq.gz ] ]
+    trim_reads_merged // channel: [ val(meta), [ fastq.gz ] ]
+    trim_read_count   // channel: [ val(meta), val(count) ]
+    fastqc_trim_html  // channel: [ val(meta), [ html ] ]
+    fastqc_trim_zip   // channel: [ val(meta), [ zip ] ]
+    versions          = ch_versions // channel: [ versions.yml ]
+}
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
new file mode 100644
index 000000000..9308fe9ba
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml
@@ -0,0 +1,129 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+# yaml-language-server: $schema=yaml-schema.json
+name: "fastq_fastqc_umitools_fastp"
+description: Read QC, UMI extraction and trimming
+keywords:
+  - fastq
+  - fastqc
+  - qc
+  - UMI
+  - trimming
+  - fastp
+components:
+  - fastqc
+  - umitools/extract
+  - fastp
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - reads:
+      type: file
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+  - skip_fastqc:
+      type: boolean
+      description: |
+        Skip fastqc process
+  - with_umi:
+      type: boolean
+      description: |
+        With or without umi detection
+  - skip_umi_extract:
+      type: boolean
+      description: |
+        With or without umi extrection
+  - umi_discard_read:
+      type: integer
+      description: |
+        Discard R1 / R2 if required
+  - skip_trimming:
+      type: boolean
+      description: |
+        Allows to skip FastP execution
+  - adapter_fasta:
+      type: file
+      description: |
+        Fasta file of adapter sequences
+  - save_trimmed_fail:
+      type: boolean
+      description: |
+        Save trimmed fastqs of failed samples
+  - save_merged:
+      type: boolean
+      description: |
+        Save merged fastqs
+  - min_trimmed_reads:
+      type: integer
+      description: |
+        Inputs with fewer than this reads will be filtered out of the "reads" output channel
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test' ]
+  - reads:
+      type: file
+      description: >
+        Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. |
+        For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz.
+      pattern: "*.{fastq.gz}"
+  - fastqc_html:
+      type: file
+      description: FastQC report
+      pattern: "*_{fastqc.html}"
+  - fastqc_zip:
+      type: file
+      description: FastQC report archive
+      pattern: "*_{fastqc.zip}"
+  - log:
+      type: file
+      description: Logfile for umi_tools
+      pattern: "*.{log}"
+  - trim_json:
+      type: file
+      description: FastP Trimming report
+      pattern: "*.{fastp.json}"
+  - trim_html:
+      type: file
+      description: FastP Trimming report
+      pattern: "*.{fastp.html}"
+  - log:
+      type: file
+      description: Logfile FastP
+      pattern: "*.{fastp.log}"
+  - trim_reads_fail:
+      type: file
+      description: Trimmed fastq files failing QC
+      pattern: "*.{fastq.gz}"
+  - trim_reads_merged:
+      type: file
+      description: Trimmed and merged fastq files
+      pattern: "*.{fastq.gz}"
+  - trim_read_count:
+      type: integer
+      description: Number of reads after trimming
+  - fastqc_trim_html:
+      type: file
+      description: FastQC report
+      pattern: "*_{fastqc.html}"
+  - fastqc_trim_zip:
+      type: file
+      description: FastQC report archive
+      pattern: "*_{fastqc.zip}"
+  - adapter_seq:
+      type: string
+      description: |
+        Adapter Sequence found in read1
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@robsyme"
+maintainers:
+  - "@robsyme"
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
new file mode 100644
index 000000000..edf325e6e
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test
@@ -0,0 +1,996 @@
+nextflow_workflow {
+
+    name "Test Workflow FASTQ_FASTQC_UMITOOLS_FASTP"
+    script "../main.nf"
+    workflow "FASTQ_FASTQC_UMITOOLS_FASTP"
+    config './nextflow.config'
+
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "subworkflows/fastq_fastqc_umitools_fastp"
+    tag "fastq_fastqc_umitools_fastp"
+    tag "fastqc"
+    tag "umitools/extract"
+    tag "fastp"
+
+
+    test("sarscov2 paired-end [fastq]") {
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | skip_fastqc") {
+
+        when {
+            params {
+                skip_fastqc       = true
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert !workflow.out.fastqc_raw_html },
+                { assert !workflow.out.fastqc_raw_zip },
+                { assert !workflow.out.fastqc_trim_html },
+                { assert !workflow.out.fastqc_trim_zip },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | with_umi") {
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = true
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+
+    test("sarscov2 paired-end [fastq] | skip_umi_extract") {
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = true
+                skip_umi_extract  = true
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | umi_discard_read = 2") {
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = true
+                skip_umi_extract  = true
+                umi_discard_read  = 2
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | skip_trimming") {
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = true
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert !workflow.out.fastqc_trim_html },
+                { assert !workflow.out.fastqc_trim_zip },
+                { assert !workflow.out.trim_html },
+                { assert !workflow.out.trim_log },
+                { assert snapshot(
+                        // If we skip trimming then input is output, so not snapshotting
+                        workflow.out.adapter_seq,
+                        workflow.out.reads.get(0).get(0), // Reads meta map
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | save_trimmed_fail") {
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = true
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = "-e 30"
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | save_merged") {
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = true
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | min_trimmed_reads = 26") {
+        // Subworkflow should stop after FASTP which trims down to 25 reads
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = true
+                min_trimmed_reads = 26
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert workflow.out.fastqc_raw_html },
+                { assert workflow.out.fastqc_raw_zip },
+                { assert workflow.out.fastqc_trim_html },
+                { assert workflow.out.fastqc_trim_zip },
+                { assert workflow.out.trim_html },
+                { assert workflow.out.trim_log },
+                { assert snapshot(
+                        workflow.out.adapter_seq,
+                        workflow.out.reads,
+                        workflow.out.trim_json,
+                        workflow.out.trim_read_count,
+                        workflow.out.trim_reads_fail,
+                        workflow.out.trim_reads_merged,
+                        workflow.out.umi_log,
+                        workflow.out.versions
+                    ).match()
+                }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] - stub") {
+        options '-stub-run'
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | skip_fastqc - stub") {
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = true
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end: false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | with_umi - stub") {
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = true
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+
+    test("sarscov2 paired-end [fastq] | skip_umi_extract - stub") {
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = true
+                skip_umi_extract  = true
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | umi_discard_read = 2 - stub") {
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = true
+                skip_umi_extract  = true
+                umi_discard_read  = 2
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | skip_trimming - stub") {
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = true
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(
+                    workflow.out.adapter_seq,
+                    workflow.out.fastqc_raw_html,
+                    workflow.out.fastqc_raw_zip,
+                    workflow.out.fastqc_trim_html,
+                    workflow.out.fastqc_trim_zip,
+                    workflow.out.trim_html,
+                    workflow.out.trim_json,
+                    workflow.out.trim_log,
+                    workflow.out.trim_read_count,
+                    workflow.out.trim_reads_fail,
+                    workflow.out.trim_reads_merged,
+                    workflow.out.umi_log,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | save_trimmed_fail - stub") {
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = true
+                save_merged       = false
+                min_trimmed_reads = 1
+                fastp_args        = "-e 30"
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | save_merged - stub") {
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = true
+                min_trimmed_reads = 1
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("sarscov2 paired-end [fastq] | min_trimmed_reads = 26 - stub") {
+        // Subworkflow should stop after FASTP which trims down to 25 reads
+        options "-stub-run"
+
+        when {
+            params {
+                skip_fastqc       = false
+                with_umi          = false
+                skip_umi_extract  = false
+                umi_discard_read  = 1
+                skip_trimming     = false
+                adapter_fasta     = []
+                save_trimmed_fail = false
+                save_merged       = true
+                min_trimmed_reads = 26
+                fastp_args        = ""
+            }
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = params.skip_fastqc
+                input[2] = params.with_umi
+                input[3] = params.skip_umi_extract
+                input[4] = params.umi_discard_read
+                input[5] = params.skip_trimming
+                input[6] = params.adapter_fasta
+                input[7] = params.save_trimmed_fail
+                input[8] = params.save_merged
+                input[9] = params.min_trimmed_reads
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
new file mode 100644
index 000000000..47c8b1d55
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap
@@ -0,0 +1,2407 @@
+{
+    "sarscov2 paired-end [fastq] | skip_umi_extract": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "unspecified"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
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+                            "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "10": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        1
+                    ]
+                ],
+                "11": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "12": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "13": [
+                    "versions.yml:md5,20ceba9ff7bc15c1fbe2b369022178ce",
+                    "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                    "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        ""
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "8": [
+                    
+                ],
+                "9": [
+                    
+                ],
+                "adapter_seq": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        ""
+                    ]
+                ],
+                "fastqc_raw_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_raw_zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_trim_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_trim_zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "trim_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_json": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_read_count": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        1
+                    ]
+                ],
+                "trim_reads_fail": [
+                    
+                ],
+                "trim_reads_merged": [
+                    
+                ],
+                "umi_log": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,20ceba9ff7bc15c1fbe2b369022178ce",
+                    "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                    "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-31T13:43:31.664576438"
+    },
+    "sarscov2 paired-end [fastq] | save_merged": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "unspecified"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    [
+                        "test_1.fastp.fastq.gz:md5,54b726a55e992a869fd3fa778afe1672",
+                        "test_2.fastp.fastq.gz:md5,29d3b33b869f7b63417b8ff07bb128ba"
+                    ]
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.fastp.json:md5,8f99097bfa04b629891105b8af9c429f"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    75
+                ]
+            ],
+            [
+                
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.merged.fastq.gz:md5,c873bb1ab3fa859dcc47306465e749d5"
+                ]
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,20ceba9ff7bc15c1fbe2b369022178ce",
+                "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-31T13:43:16.218890529"
+    },
+    "sarscov2 paired-end [fastq] | with_umi - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "10": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        1
+                    ]
+                ],
+                "11": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "12": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "13": [
+                    "versions.yml:md5,01f264f78de3c6d893c449cc6d3cd721",
+                    "versions.yml:md5,20ceba9ff7bc15c1fbe2b369022178ce",
+                    "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                    "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.umi_extract.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        ""
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "8": [
+                    
+                ],
+                "9": [
+                    
+                ],
+                "adapter_seq": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        ""
+                    ]
+                ],
+                "fastqc_raw_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_raw_zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_trim_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_trim_zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                    ]
+                ],
+                "trim_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_json": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_read_count": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        1
+                    ]
+                ],
+                "trim_reads_fail": [
+                    
+                ],
+                "trim_reads_merged": [
+                    
+                ],
+                "umi_log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.umi_extract.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,01f264f78de3c6d893c449cc6d3cd721",
+                    "versions.yml:md5,20ceba9ff7bc15c1fbe2b369022178ce",
+                    "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                    "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-31T14:19:00.760820449"
+    },
+    "sarscov2 paired-end [fastq] | skip_umi_extract - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "10": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        1
+                    ]
+                ],
+                "11": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "12": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "13": [
+                    "versions.yml:md5,20ceba9ff7bc15c1fbe2b369022178ce",
+                    "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                    "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        ""
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "7": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "8": [
+                    
+                ],
+                "9": [
+                    
+                ],
+                "adapter_seq": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        ""
+                    ]
+                ],
+                "fastqc_raw_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_raw_zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_trim_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "fastqc_trim_zip": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "reads": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        [
+                            "test_1.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940",
+                            "test_2.fastp.fastq.gz:md5,68b329da9893e34099c7d8ad5cb9c940"
+                        ]
+                    ]
+                ],
+                "trim_html": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.html:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_json": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.json:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_log": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.fastp.log:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "trim_read_count": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        1
+                    ]
+                ],
+                "trim_reads_fail": [
+                    
+                ],
+                "trim_reads_merged": [
+                    
+                ],
+                "umi_log": [
+                    
+                ],
+                "versions": [
+                    "versions.yml:md5,20ceba9ff7bc15c1fbe2b369022178ce",
+                    "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0",
+                    "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.5"
+        },
+        "timestamp": "2025-03-31T14:19:05.849804123"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config
new file mode 100644
index 000000000..428b0d37c
--- /dev/null
+++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config
@@ -0,0 +1,16 @@
+process {
+
+    withName: UMITOOLS_EXTRACT {
+        ext.args = '--bc-pattern="NNNN" --bc-pattern2="NNNN"'
+    }
+
+    withName: UMICOLLAPSE {
+        ext.prefix = { "${meta.id}.dedup" }
+    }
+
+    // Make filtering more aggressive to make more reads fail
+    withName: FASTP {
+        ext.args = params.fastp_args
+    }
+
+}
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 689fb5f67..4bdf1c518 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -285,6 +285,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-20T19:03:37.62529837"
+        "timestamp": "2025-08-21T16:53:43.678975494"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 759d060d9..8a06326d3 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -9,7 +9,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -218,7 +218,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:11:25.285702343"
+        "timestamp": "2025-08-21T18:20:40.211602465"
     },
     "stub test stringtie": {
         "content": [
@@ -230,7 +230,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -364,7 +364,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:20:46.857913005"
+        "timestamp": "2025-08-21T18:21:21.812203485"
     },
     "stub test arriba": {
         "content": [
@@ -379,7 +379,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -503,7 +503,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:16:32.896368369"
+        "timestamp": "2025-08-21T18:15:00.863385937"
     },
     "stub test fusioninspector": {
         "content": [
@@ -524,7 +524,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONINSPECTOR": {
@@ -777,7 +777,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:16:11.496999226"
+        "timestamp": "2025-08-21T18:31:32.043336569"
     },
     "stub test salmon": {
         "content": [
@@ -789,7 +789,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -872,7 +872,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:22:26.942424142"
+        "timestamp": "2025-08-21T18:27:42.592742898"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -890,7 +890,7 @@
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -1109,7 +1109,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:08:54.904059765"
+        "timestamp": "2025-08-21T19:00:06.777067929"
     },
     "stub test fusionreport": {
         "content": [
@@ -1124,7 +1124,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1363,7 +1363,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:13:31.815555556"
+        "timestamp": "2025-08-21T18:26:22.470758896"
     },
     "stub test fastp": {
         "content": [
@@ -1378,10 +1378,10 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
-                "FASTQC_FOR_FASTP": {
+                "FASTQC_TRIM": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -1417,9 +1417,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
@@ -1443,7 +1440,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:21:51.143010384"
+        "timestamp": "2025-08-21T18:27:03.13267105"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1455,7 +1452,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1515,7 +1512,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:18:29.46107784"
+        "timestamp": "2025-08-21T18:16:52.604596162"
     },
     "stub test fusioncatcher trim": {
         "content": [
@@ -1530,7 +1527,7 @@
                 "FASTP_FOR_FUSIONCATCHER": {
                     "fastp": "0.24.0"
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1596,7 +1593,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:19:01.10521868"
+        "timestamp": "2025-08-21T18:17:29.899160389"
     },
     "stub test all": {
         "content": [
@@ -1626,10 +1623,10 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
-                "FASTQC_FOR_FASTP": {
+                "FASTQC_TRIM": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1748,9 +1745,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
@@ -1965,6 +1959,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:02:59.744224309"
+        "timestamp": "2025-08-21T17:56:08.906503771"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test b/tests/test_stub_bam.nf.test
index 46c06d9df..036540242 100644
--- a/tests/test_stub_bam.nf.test
+++ b/tests/test_stub_bam.nf.test
@@ -258,7 +258,6 @@ nextflow_pipeline {
                 input            = "${projectDir}/tests/yml/bam.yml"
                 no_cosmic        = true
                 genomes_base     = "${outputDir}/references"
-                pfam_file        = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
                 arriba_ref_protein_domains  = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"
                 arriba_ref_known_fusions    = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/known_fusions_hg38_GRCh38_v2.5.0.tsv.gz"
                 arriba_ref_blacklist        = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 762751f28..944981d1c 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -9,7 +9,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -191,7 +191,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T15:54:01.219397324"
+        "timestamp": "2025-08-21T18:43:00.825098336"
     },
     "stub test stringtie": {
         "content": [
@@ -203,7 +203,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -313,7 +313,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:34:57.146117646"
+        "timestamp": "2025-08-21T18:43:40.299953119"
     },
     "stub test arriba": {
         "content": [
@@ -328,7 +328,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -425,7 +425,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:31:27.759259528"
+        "timestamp": "2025-08-21T18:34:38.598054226"
     },
     "stub test fusioninspector": {
         "content": [
@@ -446,7 +446,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONINSPECTOR": {
@@ -675,7 +675,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:00:15.182860428"
+        "timestamp": "2025-08-21T19:08:28.888308012"
     },
     "stub test salmon": {
         "content": [
@@ -687,7 +687,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -789,7 +789,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:37:16.616916274"
+        "timestamp": "2025-08-21T18:45:12.252856442"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -807,7 +807,7 @@
                 "CTATSPLICING_STARTOCANCERINTRONS": {
                     "ctat-splicing": "0.0.2"
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -1002,7 +1002,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T15:47:14.034109802"
+        "timestamp": "2025-08-21T18:37:51.326973268"
     },
     "stub test fusionreport": {
         "content": [
@@ -1017,7 +1017,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1229,7 +1229,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T15:56:39.559031879"
+        "timestamp": "2025-08-21T19:14:34.336489702"
     },
     "stub test fastp": {
         "content": [
@@ -1244,10 +1244,10 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
-                "FASTQC_FOR_FASTP": {
+                "FASTQC_TRIM": {
                     "fastqc": "0.12.1"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
@@ -1293,9 +1293,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
@@ -1328,7 +1325,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:36:44.624772922"
+        "timestamp": "2025-08-21T18:44:39.897883035"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1340,7 +1337,7 @@
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1419,7 +1416,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:33:11.268038905"
+        "timestamp": "2025-08-21T18:38:43.272605368"
     },
     "stub test all": {
         "content": [
@@ -1449,10 +1446,10 @@
                 "FASTP": {
                     "fastp": "0.24.0"
                 },
-                "FASTQC": {
+                "FASTQC_RAW": {
                     "fastqc": "0.12.1"
                 },
-                "FASTQC_FOR_FASTP": {
+                "FASTQC_TRIM": {
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
@@ -1563,9 +1560,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "fastqc_for_fastp",
-                "fastqc_for_fastp/test_trimmed.html",
-                "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
@@ -1764,6 +1758,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T15:39:59.605183405"
+        "timestamp": "2025-08-21T18:33:36.953695887"
     }
 }
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 06a5dd26c..e5d642121 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -6,7 +6,7 @@
 
 include { BUILD_REFERENCES              }   from '../subworkflows/local/build_references'
 include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/main'
-include { TRIM_WORKFLOW                 }   from '../subworkflows/local/trim_workflow/main'
+include { FASTQ_FASTQC_UMITOOLS_FASTP   }   from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp/main'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
 include { STARFUSION_DETECT             }   from '../modules/nf-core/starfusion/detect/main'
 include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
@@ -115,37 +115,49 @@ workflow RNAFUSION {
         }
 
         //
-        // QC from FASTQ files
+        // SUBWORKFLOW: Read QC, optional UMI extraction, and trimming (nf-core)
         //
 
-        if(!params.skip_qc) {
-            FASTQC (
-                ch_fastqs_to_process.found,
-            )
-            ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]})
-            ch_versions = ch_versions.mix(FASTQC.out.versions)
-        }
+        def ch_reads = Channel.empty()
 
-        //
-        // SUBWORKFLOW: Trimming
-        //
+        // adapter_fasta as a channel (or empty if not provided)
+        def ch_adapter_fasta = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta).collect() : []
+
+        // control flags mapped to the nf-core subworkflow: disable umi usage in this pipeline
+        def with_umi         = false
+        def skip_umi_extract = false
+        def umi_discard_read = 0
+
+        // if 'fastp' isn't selected, we still run the subworkflow but skip trimming;
+        def skip_trimming    = (!tools.contains("fastp"))
+
+        // optional fastp output controls + minimum reads after trimming
+        def save_trimmed_fail = params.save_trimmed_fail ?: false
+        def save_merged       = params.save_merged ?: false
+        def min_trimmed_reads = (params.min_trimmed_reads ?: 1) as Integer
+
+        FASTQ_FASTQC_UMITOOLS_FASTP(
+            ch_fastqs_to_process.found,  // reads: [ val(meta), [fastqs] ]
+            params.skip_qc,              // skip_fastqc
+            with_umi,                    // with_umi
+            skip_umi_extract,            // skip_umi_extract
+            umi_discard_read,            // umi_discard_read (0,1,2)
+            skip_trimming,               // skip_trimming
+            ch_adapter_fasta,            // adapter_fasta
+            save_trimmed_fail,           // save_trimmed_fail
+            save_merged,                 // save_merged
+            min_trimmed_reads            // min_trimmed_reads
+        )
 
-        def ch_reads = Channel.empty()
-        if(tools.contains("fastp")) {
-            def ch_adapter_fasta = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta).collect() : []
-            TRIM_WORKFLOW (
-                ch_fastqs_to_process.found,
-                ch_adapter_fasta,
-                params.skip_qc
-            )
-            ch_reads = TRIM_WORKFLOW.out.ch_reads_all
-            ch_versions      = ch_versions.mix(TRIM_WORKFLOW.out.versions)
-            ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_html.collect{it[1]})
-            ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastp_json.collect{it[1]})
-            ch_multiqc_files = ch_multiqc_files.mix(TRIM_WORKFLOW.out.ch_fastqc_trimmed.collect{it[1]})
-        } else {
-            ch_reads = ch_fastqs_to_process.found
-        }
+        // primary reads for downstream tools
+        ch_reads    = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
+        ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
+
+        // feed MultiQC with outputs (if not skipped)
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{ it[1] }.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{ it[1] }.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_html.collect{ it[1] }.ifEmpty([]))
+        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{ it[1] }.ifEmpty([]))
 
         //
         // MODULE: SALMON_QUANT

From 0d0c739bae96ceb265d60882779b672c9af4cc4d Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 21 Aug 2025 19:41:37 +0000
Subject: [PATCH 783/887] fix: replace for new module names in config

---
 conf/modules.config | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 8e0ba6060..a55f8d418 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -85,7 +85,7 @@ process {
         ]
     }
 
-    withName: 'FASTQC' {
+    withName: 'FASTQC_RAW' {
         ext.args = '--quiet'
         publishDir = [
             path: { "${params.outdir}/fastqc" },
@@ -94,7 +94,7 @@ process {
         ]
     }
 
-    withName: 'FASTQC_FOR_FASTP' {
+    withName: 'FASTQC_TRIM' {
         ext.args = '--quiet'
         ext.prefix = { "${meta.id}_trimmed" }
         publishDir = [

From fcbf9dbb0fd2da0a580e43389e6ac9a3b44594c3 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 21 Aug 2025 22:52:29 +0000
Subject: [PATCH 784/887] test: update snapshot

---
 tests/test_stub.nf.test.snap     | 10 ++++++++--
 tests/test_stub_bam.nf.test.snap | 10 ++++++++--
 2 files changed, 16 insertions(+), 4 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 8a06326d3..dba1ba99c 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1417,6 +1417,9 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
@@ -1440,7 +1443,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:27:03.13267105"
+        "timestamp": "2025-08-21T22:48:35.622133369"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1745,6 +1748,9 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
@@ -1959,6 +1965,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T17:56:08.906503771"
+        "timestamp": "2025-08-21T22:48:04.457572169"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 944981d1c..6bf0d5266 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -1293,6 +1293,9 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
                 "gatk4",
                 "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
@@ -1325,7 +1328,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:44:39.897883035"
+        "timestamp": "2025-08-21T22:51:21.946095021"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1560,6 +1563,9 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
+                "fastqc_for_fastp",
+                "fastqc_for_fastp/test_trimmed.html",
+                "fastqc_for_fastp/test_trimmed.zip",
                 "fusioncatcher",
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
@@ -1758,6 +1764,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:33:36.953695887"
+        "timestamp": "2025-08-21T22:50:42.87458983"
     }
 }
\ No newline at end of file

From 556028273fbec0afbdb79a0aac6f25a15d8e14c7 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 22 Aug 2025 14:14:35 +0000
Subject: [PATCH 785/887] style: improve ifempty usage

---
 workflows/rnafusion.nf | 20 +++++++++++---------
 1 file changed, 11 insertions(+), 9 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index e5d642121..ac68230e5 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -115,7 +115,7 @@ workflow RNAFUSION {
         }
 
         //
-        // SUBWORKFLOW: Read QC, optional UMI extraction, and trimming (nf-core)
+        // SUBWORKFLOW: Read QC and trimming (nf-core)
         //
 
         def ch_reads = Channel.empty()
@@ -123,12 +123,12 @@ workflow RNAFUSION {
         // adapter_fasta as a channel (or empty if not provided)
         def ch_adapter_fasta = params.adapter_fasta ? Channel.fromPath(params.adapter_fasta).collect() : []
 
-        // control flags mapped to the nf-core subworkflow: disable umi usage in this pipeline
+        // disable umi usage in this subworkflow for this pipeline
         def with_umi         = false
         def skip_umi_extract = false
         def umi_discard_read = 0
 
-        // if 'fastp' isn't selected, we still run the subworkflow but skip trimming;
+        // if 'fastp' isn't selected, we still run the subworkflow but skip trimming
         def skip_trimming    = (!tools.contains("fastp"))
 
         // optional fastp output controls + minimum reads after trimming
@@ -149,15 +149,17 @@ workflow RNAFUSION {
             min_trimmed_reads            // min_trimmed_reads
         )
 
-        // primary reads for downstream tools
         ch_reads    = FASTQ_FASTQC_UMITOOLS_FASTP.out.reads
         ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions)
 
-        // feed MultiQC with outputs (if not skipped)
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.collect{ it[1] }.ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.collect{ it[1] }.ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_html.collect{ it[1] }.ifEmpty([]))
-        ch_multiqc_files = ch_multiqc_files.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.collect{ it[1] }.ifEmpty([]))
+        ch_sbwf_fastp_mqc = Channel.empty()
+            .mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_raw_zip.map { it[1] })
+            .mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.fastqc_trim_zip.map { it[1] })
+            .mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_html.map { it[1] })
+            .mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.trim_json.map { it[1] })
+            .ifEmpty([])
+
+        ch_multiqc_files = ch_multiqc_files.mix(ch_sbwf_fastp_mqc)
 
         //
         // MODULE: SALMON_QUANT

From 82dbc7cc9cb90fd424119ebb5d81c3e8a2f36d0f Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 22 Aug 2025 14:15:03 +0000
Subject: [PATCH 786/887] chore: remove unused parameters, add new trim params

---
 nextflow.config      |  7 +++----
 nextflow_schema.json | 38 +++++++++++++++++++++++---------------
 2 files changed, 26 insertions(+), 19 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 48a8badd1..3bea66b33 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -40,6 +40,9 @@ params {
     trim_tail                  = 0
     trim_tail_fusioncatcher    = 0
     adapter_fasta              = []
+    save_trimmed_fail          = false
+    save_merged                = false
+    min_trimmed_reads          = 1
 
     // Compression
     cram                       = false
@@ -60,10 +63,6 @@ params {
     skip_vis                   = false
     skip_vcf                   = false
 
-    // Download references option
-    download_refs               = false
-    fusioncatcher_download_link = "1UKFlyqMPVF7HBqfrgS6yoxgHoqfA-wbo"
-
     // Reference options
     no_cosmic                     = false
     ctatsplicing_cancer_introns   = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/CANCER_SPLICING_LIB_SUPPLEMENT/cancer_introns.GRCh38.Jun232020.tsv.gz"
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 3c5cf1498..5b15693b1 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -13,15 +13,18 @@
             "properties": {
                 "skip_qc": {
                     "type": "boolean",
-                    "description": "Skip QC steps"
+                    "description": "Skip QC steps",
+                    "fa_icon": "fas fa-forward"
                 },
                 "skip_vcf": {
                     "type": "boolean",
-                    "description": "Skip vcf creation step"
+                    "description": "Skip vcf creation step",
+                    "fa_icon": "fas fa-forward"
                 },
                 "skip_vis": {
                     "type": "boolean",
-                    "description": "Skip visualisation steps"
+                    "description": "Skip visualisation steps",
+                    "fa_icon": "fas fa-forward"
                 }
             },
             "fa_icon": "fas fa-fast-forward"
@@ -135,11 +138,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to arriba output"
                 },
-                "download_refs": {
-                    "type": "boolean",
-                    "fa_icon": "far fa-file-code",
-                    "description": "Download references instead of building them (for fusioncatcher and starfusion)"
-                },
                 "fusioncatcher_fusions": {
                     "type": "string",
                     "format": "file-path",
@@ -161,11 +159,6 @@
                     "fa_icon": "far fa-file-code",
                     "description": "Path to fusioncatcher references"
                 },
-                "fusioncatcher_download_link": {
-                    "type": "string",
-                    "description": "Fusioncatcher download link",
-                    "fa_icon": "far fa-file-code"
-                },
                 "fusioninspector_limitSjdbInsertNsj": {
                     "type": "integer",
                     "fa_icon": "far fa-file-code",
@@ -339,6 +332,19 @@
                     "pattern": "\\S+\\.f(n|ast)?a$",
                     "description": "Path to adapter fasta file: default: []",
                     "fa_icon": "fas fa-cut"
+                },
+                "save_trimmed_fail": {
+                    "type": "boolean",
+                    "fa_icon": "far fa-times-circle"
+                },
+                "min_trimmed_reads": {
+                    "type": "integer",
+                    "default": 1,
+                    "fa_icon": "fas fa-search-minus"
+                },
+                "save_merged": {
+                    "type": "boolean",
+                    "fa_icon": "fas fa-download"
                 }
             }
         },
@@ -429,11 +435,13 @@
                 },
                 "dfam_version": {
                     "type": ["number", "null"],
-                    "description": "Version of dfam to use"
+                    "description": "Version of dfam to use",
+                    "fa_icon": "fas fa-code-branch"
                 },
                 "pfam_version": {
                     "type": ["number", "null"],
-                    "description": "Version of pfam to use"
+                    "description": "Version of pfam to use",
+                    "fa_icon": "fas fa-code-branch"
                 }
             }
         },

From cc302d69afcdcce5d09fd38061672d4dce1fc8b2 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 22 Aug 2025 14:18:41 +0000
Subject: [PATCH 787/887] chore: remove unused file

---
 adapter_fasta_test | 0
 1 file changed, 0 insertions(+), 0 deletions(-)
 delete mode 100644 adapter_fasta_test

diff --git a/adapter_fasta_test b/adapter_fasta_test
deleted file mode 100644
index e69de29bb..000000000

From 889c219a96676a57bca5b08074052d4b6abc0307 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 22 Aug 2025 14:18:49 +0000
Subject: [PATCH 788/887] docs: update changelog

---
 CHANGELOG.md | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1837f0ef4..c6aac2f82 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -91,6 +91,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
 - Modified `test_build` profile to use a reduced version of Pfam and Dfam files [#733](https://github.com/nf-core/rnafusion/pull/733)
 - Changed local `ARRIBA_VISUALIZATION`, `CTATSPLICING_STARTOCANCERINTRONS`, `CTATSPLICING_PREPGENOMELIB`, `FUSIONINSPECTOR`, `STARFUSION_DETECT` for its nf-core module versions [#740](https://github.com/nf-core/rnafusion/pull/740)
+- Replaced local subworkflow `TRIM_WORKFLOW` for its nf-core subworkflow equivalent `FASTQ_FASTQC_UMITOOLS_FASTP` [#752](https://github.com/nf-core/rnafusion/pull/752)
 
 ### Fixed
 
@@ -127,6 +128,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Removed subworkflow with less than two modules: `CTATSPLICING_WORKFLOW` [#704](https://github.com/nf-core/rnafusion/pull/704)
 - Removed subworkflow with less than two modules: `FUSIONREPORT_WORKFLOW` [#721](https://github.com/nf-core/rnafusion/pull/721)
 - Removed local module `GET_RRNA_TRANSCRIPTS` [#736](https://github.com/nf-core/rnafusion/pull/736)
+- Removed old unused parameters `params.download_refs` and `params.fusioncatcher_download_link` [#752](https://github.com/nf-core/rnafusion/pull/752)
 
 ### Parameters
 
@@ -142,6 +144,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | `--all`               | `--tools all`                   |
 |                       | `--fusioncatcher_download_link` |
 |                       | `--trim_tail_fusioncatcher`     |
+|                       | `--save_trimmed_fail`           |
+|                       | `--save_merged`                 |
+|                       | `--min_trimmed_reads`           |
+|                       | `--trim_tail_fusioncatcher`     |
 
 ## v3.0.2 - [2024-04-10]
 

From 6057891f77508e09e01e0b26cf1edebe2aa232ee Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Fri, 22 Aug 2025 14:32:03 +0000
Subject: [PATCH 789/887] chore: remove non-existent test

---
 nextflow.config | 3 ---
 1 file changed, 3 deletions(-)

diff --git a/nextflow.config b/nextflow.config
index 3bea66b33..ef54ad4e6 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -212,9 +212,6 @@ profiles {
     test_build {
         includeConfig 'conf/test_build.config'
     }
-    test_cosmic {
-        includeConfig 'conf/test_cosmic.config'
-    }
     test_full {
         includeConfig 'conf/test_full.config'
     }

From 8205a982d82f72a54751dcd9bff538176d9a8887 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 25 Aug 2025 13:34:17 +0200
Subject: [PATCH 790/887] intermetiate snap update

---
 tests/test_stub_bam.nf.test.snap | 218 +------------------------------
 1 file changed, 6 insertions(+), 212 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 041d94837..aa50a39e4 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -884,212 +884,22 @@
     },
     "stub test ctatsplicing": {
         "content": [
-            16,
+            0,
             {
-                "CTATSPLICING_PREPGENOMELIB": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "CTATSPLICING_STARTOCANCERINTRONS": {
-                    "ctat-splicing": "0.0.2"
-                },
-                "FASTQC": {
-                    "fastqc": "0.12.1"
-                },
-                "GATK4_BEDTOINTERVALLIST": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
-                },
-                "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
-                },
-                "GENCODE_DOWNLOAD": {
-                    "wget": "1.21.4"
-                },
-                "GET_RRNA_TRANSCRIPTS": {
-                    "get_rrna_transcripts": "v1.0"
-                },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
-                "PICARD_COLLECTINSERTSIZEMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "PICARD_COLLECTRNASEQMETRICS": {
-                    "picard": "3.3.0"
-                },
-                "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
-                },
-                "STARFUSION_BUILD": {
-                    "gunzip": 1.12,
-                    "hmmer": 3.4,
-                    "STAR-Fusion": "1.15.1"
-                },
-                "STAR_GENOMEGENERATE": {
-                    "star": "2.7.11b",
-                    "samtools": 1.21,
-                    "gawk": "5.1.0"
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "ctatsplicing",
-                "ctatsplicing/test.cancer.introns",
-                "ctatsplicing/test.cancer.introns.prelim",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam",
-                "ctatsplicing/test.cancer_intron_reads.sorted.bam.bai",
-                "ctatsplicing/test.chckpts",
-                "ctatsplicing/test.ctat-splicing.igv.html",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam",
-                "ctatsplicing/test.gene_reads.sorted.sifted.bam.bai",
-                "ctatsplicing/test.igv.tracks",
-                "ctatsplicing/test.introns",
-                "ctatsplicing/test.introns.for_IGV.bed",
-                "fastqc",
-                "fastqc/test.html",
-                "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
-                "get",
-                "get/rrna.bed",
-                "get/rrna.gtf",
-                "multiqc",
-                "multiqc/multiqc_data",
-                "multiqc/multiqc_plots",
-                "multiqc/multiqc_report.html",
-                "picard",
-                "picard/test.bai",
-                "picard/test.bam",
-                "picard/test.bam.metrics",
-                "picard/test.cram",
-                "picard/test.cram.crai",
-                "picard/test.rna_metrics",
-                "picard/test_collectinsertsize.pdf",
-                "picard/test_collectinsertsize.txt",
                 "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
-                "references",
-                "references/GRCh38",
-                "references/GRCh38/gencode_v46",
-                "references/GRCh38/gencode_v46/gencode",
-                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
-                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
-                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
-                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
-                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
-                "references/GRCh38/gencode_v46/star",
-                "references/GRCh38/gencode_v46/star/Genome",
-                "references/GRCh38/gencode_v46/star/Log.out",
-                "references/GRCh38/gencode_v46/star/SA",
-                "references/GRCh38/gencode_v46/star/SAindex",
-                "references/GRCh38/gencode_v46/star/chrLength.txt",
-                "references/GRCh38/gencode_v46/star/chrName.txt",
-                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
-                "references/GRCh38/gencode_v46/star/chrStart.txt",
-                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
-                "references/GRCh38/gencode_v46/star/exonInfo.tab",
-                "references/GRCh38/gencode_v46/star/geneInfo.tab",
-                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
-                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
-                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
-                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
-                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
-                "references/GRCh38/gencode_v46/starfusion",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.bed",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
-                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
-                "star",
-                "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai"
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T15:07:27.162452256"
+        "timestamp": "2025-08-08T21:23:14.68337"
     },
     "stub test fusionreport": {
         "content": [
@@ -1498,17 +1308,11 @@
     },
     "stub test all": {
         "content": [
-            33,
+            30,
             {
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.1"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.5.0"
-                },
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctat-splicing": "0.0.2"
                 },
@@ -1595,22 +1399,14 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0",
-                    "vcf_collect": 0.1
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-                "agat",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "ctatsplicing",
                 "ctatsplicing/test.cancer.introns",
                 "ctatsplicing/test.cancer.introns.prelim",
@@ -1937,15 +1733,13 @@
                 "stringtie/test.ballgown",
                 "stringtie/test.coverage.gtf",
                 "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf",
-                "vcf",
-                "vcf/test_fusion_data.vcf"
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-07T13:56:14.46501"
+        "timestamp": "2025-08-08T14:46:39.887774"
     }
 }
\ No newline at end of file

From 766b0f8819fd5bf300daa6f81a6573d83b7b4dd8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 25 Aug 2025 16:27:16 +0200
Subject: [PATCH 791/887] update snap

---
 tests/test_stub_bam.nf.test.snap | 192 ++++++++++++++++++++-----------
 1 file changed, 124 insertions(+), 68 deletions(-)

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index bebbd6aed..71462b2f1 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -191,7 +191,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T15:54:01.219397324"
+        "timestamp": "2025-08-25T15:52:40.204999"
     },
     "stub test stringtie": {
         "content": [
@@ -313,7 +313,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:34:57.146117646"
+        "timestamp": "2025-08-25T15:54:38.734653"
     },
     "stub test arriba": {
         "content": [
@@ -425,24 +425,18 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:31:27.759259528"
+        "timestamp": "2025-08-25T15:31:45.044653"
     },
     "stub test fusioninspector": {
         "content": [
-            23,
+            20,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
                 },
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.1"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
@@ -496,10 +490,6 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0",
-                    "vcf_collect": 0.1
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -507,12 +497,9 @@
             [
                 "agat",
                 "agat/Homo_sapiens.GRCh38.46.gtf.bed",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "bioawk",
                 "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
@@ -666,16 +653,14 @@
                 "references/GRCh38/hgnc/hgnc_complete_set.txt",
                 "star",
                 "star/test.Aligned.sortedByCoord.out.bam",
-                "star/test.Aligned.sortedByCoord.out.bam.bai",
-                "vcf",
-                "vcf/test_fusion_data.vcf"
+                "star/test.Aligned.sortedByCoord.out.bam.bai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:00:15.182860428"
+        "timestamp": "2025-08-25T16:24:25.434722"
     },
     "stub test salmon": {
         "content": [
@@ -789,14 +774,10 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:37:16.616916274"
+        "timestamp": "2025-08-25T16:11:36.786416"
     },
     "stub test ctatsplicing": {
         "content": [
-<<<<<<< HEAD
-            0,
-            {
-=======
             17,
             {
                 "AGAT_CONVERTGFF2BED": {
@@ -849,14 +830,11 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
->>>>>>> dev
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
             },
             [
-<<<<<<< HEAD
-=======
                 "agat",
                 "agat/Homo_sapiens.GRCh38.46.gtf.bed",
                 "bioawk",
@@ -891,20 +869,125 @@
                 "picard/test.rna_metrics",
                 "picard/test_collectinsertsize.pdf",
                 "picard/test_collectinsertsize.txt",
->>>>>>> dev
                 "pipeline_info",
-                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml"
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.gene_spans.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/cancer_splicing_lib/cancer_splicing.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/refGene.sort.bed.gz.tbi",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-<<<<<<< HEAD
-        "timestamp": "2025-08-08T21:23:14.68337"
-=======
-        "timestamp": "2025-08-18T15:47:14.034109802"
->>>>>>> dev
+        "timestamp": "2025-08-25T15:38:56.983178"
     },
     "stub test fusionreport": {
         "content": [
@@ -1131,7 +1214,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T15:56:39.559031879"
+        "timestamp": "2025-08-25T16:07:55.701763"
     },
     "stub test fastp": {
         "content": [
@@ -1230,7 +1313,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:36:44.624772922"
+        "timestamp": "2025-08-25T16:09:49.404204"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1321,35 +1404,21 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-08T11:33:11.268038905"
+        "timestamp": "2025-08-25T15:40:46.18532"
     },
     "stub test all": {
         "content": [
-<<<<<<< HEAD
-            30,
-            {
-                "ARRIBA_ARRIBA": {
-                    "arriba": "2.5.0"
-                },
-=======
-            34,
+            31,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
                 },
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.1"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
->>>>>>> dev
                 "CTATSPLICING_PREPGENOMELIB": {
                     "ctatsplicing": "0.0.2"
                 },
@@ -1438,22 +1507,13 @@
                 }
             },
             [
-<<<<<<< HEAD
-                "arriba",
-                "arriba/test.arriba.fusions.discarded.tsv",
-                "arriba/test.arriba.fusions.tsv",
-=======
                 "agat",
                 "agat/Homo_sapiens.GRCh38.46.gtf.bed",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "bioawk",
                 "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
->>>>>>> dev
                 "ctatsplicing",
                 "ctatsplicing/test.cancer.introns",
                 "ctatsplicing/test.cancer.introns.prelim",
@@ -1674,10 +1734,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-<<<<<<< HEAD
-        "timestamp": "2025-08-08T14:46:39.887774"
-=======
-        "timestamp": "2025-08-18T15:39:59.605183405"
->>>>>>> dev
+        "timestamp": "2025-08-25T15:29:52.456432"
     }
 }
\ No newline at end of file

From a2335c2f9179dc67e98d94cbb0014e82f60ce6fe Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Mon, 25 Aug 2025 18:38:33 +0200
Subject: [PATCH 792/887] Add test for fusioninspector subworkflow

---
 .../main.nf}                                  |  10 +-
 .../tests/main.nf.test                        | 192 ++++++++++++++++++
 .../tests/nextflow.config                     |  34 ++++
 3 files changed, 231 insertions(+), 5 deletions(-)
 rename subworkflows/local/{fusioninspector_workflow.nf => fusioninspector_workflow/main.nf} (91%)
 create mode 100644 subworkflows/local/fusioninspector_workflow/tests/main.nf.test
 create mode 100644 subworkflows/local/fusioninspector_workflow/tests/nextflow.config

diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow/main.nf
similarity index 91%
rename from subworkflows/local/fusioninspector_workflow.nf
rename to subworkflows/local/fusioninspector_workflow/main.nf
index 39ac4144a..ec6e4fafd 100644
--- a/subworkflows/local/fusioninspector_workflow.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -1,8 +1,8 @@
-include { AGAT_CONVERTSPGFF2TSV     }                     from '../../modules/nf-core/agat/convertspgff2tsv/main'
-include { ARRIBA_VISUALISATION     }                      from '../../modules/nf-core/arriba/visualisation/main'
-include { CAT_CAT }                                       from '../../modules/nf-core/cat/cat/main'
-include { VCF_COLLECT }                                   from '../../modules/local/vcf_collect/main'
-include { FUSIONINSPECTOR     }                           from '../../modules/nf-core/fusioninspector/main'
+include { AGAT_CONVERTSPGFF2TSV     }                     from '../../../modules/nf-core/agat/convertspgff2tsv/main'
+include { ARRIBA_VISUALISATION     }                      from '../../../modules/nf-core/arriba/visualisation/main'
+include { CAT_CAT }                                       from '../../../modules/nf-core/cat/cat/main'
+include { VCF_COLLECT }                                   from '../../../modules/local/vcf_collect/main'
+include { FUSIONINSPECTOR     }                           from '../../../modules/nf-core/fusioninspector/main'
 
 workflow FUSIONINSPECTOR_WORKFLOW {
     take:
diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
new file mode 100644
index 000000000..0e3930b83
--- /dev/null
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
@@ -0,0 +1,192 @@
+nextflow_workflow {
+
+    name "Test Workflow FUSIONINSPECTOR_WORKFLOW"
+    script "../main.nf"
+    workflow "FUSIONINSPECTOR_WORKFLOW"
+    config './nextflow.config'
+
+     test("FUSIONINSPECTOR_WORKFLOW - human") {
+        setup {
+            run("STARFUSION_BUILD") {
+                script "../../../../modules/nf-core/starfusion/build/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'minigenome_fasta' ],
+                        file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa")
+                    ]
+                    input[1] = [
+                        [ id:'minigenome_gtf' ],
+                        file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf")
+                    ]
+                    input[2] = file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                    input[3] = "homo_sapiens"
+                    input[3] = "homo_sapiens"
+                    input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
+                    input[5] = [
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
+                    ]
+                    input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                    """
+                }
+            }
+
+            run("FUSIONREPORT_DOWNLOAD") {
+                script "../../../../modules/nf-core/fusionreport/download/main.nf"
+                process {
+                    """
+                    """
+                }
+            }
+
+            run("FUSIONREPORT_DETECT") {
+                script "../../../../modules/nf-core/fusionreport/detect/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_sample' ], // meta map
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/arriba.tsv', checkIfExists: true),
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/starfusion.tsv', checkIfExists: true),
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/fusioncatcher.txt', checkIfExists: true)
+                    ])
+                    input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
+                    input[2] = 1
+                    """
+                }
+            }
+        }
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of(
+                    [ [ id:'test_sample' ],
+                    [
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                    ]
+                ])
+                input[1] = FUSIONREPORT_DETECT.out.fusion_list
+                input[2] = FUSIONREPORT_DETECT.out.fusion_list_filtered
+                input[3] = FUSIONREPORT_DETECT.out.report
+                input[4] = FUSIONREPORT_DETECT.out.csv
+                input[5] = [ [], [], [] ]
+                input[6] = Channel.of(
+                    [ [ id:'test_gtf' ],
+                    file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf")])
+                input[7] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"))
+                input[8] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"))
+                input[9] = Channel.of([[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")] )
+                input[10] = Channel.of([[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")] )
+                input[11] = STARFUSION_BUILD.out.reference
+                input[12] = true
+                input[13] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("FUSIONINSPECTOR_WORKFLOW - human - stub") {
+        options "-stub"
+        setup {
+            run("STARFUSION_BUILD") {
+                script "../../../../modules/nf-core/starfusion/build/main.nf"
+                process {
+                    """
+                    input[0] = [
+                        [ id:'minigenome_fasta' ],
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.fa')
+                    ]
+                    input[1] = [
+                        [ id:'minigenome_gtf' ],
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.gtf')
+                    ]
+                    input[2] = file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
+                    input[3] = "homo_sapiens"
+                    input[3] = "homo_sapiens"
+                    input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
+                    input[5] = [
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3f",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3i",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3m",
+                        "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm.h3p"
+                    ]
+                    input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
+                    """
+                }
+            }
+
+            run("FUSIONREPORT_DOWNLOAD") {
+                script "../../../../modules/nf-core/fusionreport/download/main.nf"
+                process {
+                    """
+                    """
+                }
+            }
+
+            run("FUSIONREPORT_DETECT") {
+                script "../../../../modules/nf-core/fusionreport/detect/main.nf"
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test_sample' ], // meta map
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/arriba.tsv', checkIfExists: true),
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/starfusion.tsv', checkIfExists: true),
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/fusioncatcher.txt', checkIfExists: true)
+                    ])
+                    input[1] = FUSIONREPORT_DOWNLOAD.out.fusionreport_ref
+                    input[2] = 1
+                    """
+                }
+            }
+        }
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of(
+                    [ [ id:'test_sample' ],
+                    [
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_1.fastq.gz"),
+                        file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
+                    ]
+                ])
+                input[1] = FUSIONREPORT_DETECT.out.fusion_list
+                input[2] = FUSIONREPORT_DETECT.out.fusion_list_filtered
+                input[3] = FUSIONREPORT_DETECT.out.report
+                input[4] = FUSIONREPORT_DETECT.out.csv
+                input[5] = [ [], [], [] ]
+                input[6] = Channel.of(
+                    [ [ id:'test_gtf' ],
+                    file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf")])
+                input[7] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"))
+                input[8] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"))
+                input[9] = Channel.of([[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")] )
+                input[10] = Channel.of([[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")] )
+                input[11] = STARFUSION_BUILD.out.reference
+                input[12] = true
+                input[13] = false
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
new file mode 100644
index 000000000..25a00a186
--- /dev/null
+++ b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
@@ -0,0 +1,34 @@
+params {
+    arriba                     = true
+    starfusion                 = true
+    fusioncatcher              = true
+    no_cosmic                  = true
+    tools_cutoff               = 1
+    whitelist                  = null
+    outdir                     = "test"
+    genomes_base               = "test"
+    fusionreport_download_args = '--no-cosmic'
+    fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
+    fusionreport_detect_args2  = "--export csv"
+}
+
+process {
+    withName: 'FUSIONREPORT_DOWNLOAD' {
+        ext.args         =  params.fusionreport_download_args
+    }
+
+    withName: 'FUSIONREPORT_DETECT' {
+        ext.args         = params.fusionreport_detect_args
+        ext.args2        = params.fusionreport_detect_args2
+    }
+
+    withName: 'FUSIONINSPECTOR' {
+        ext.args = '--annotate --examine_coding_effect'
+    }
+
+    resourceLimits = [
+        cpus: 6,
+        memory: '36.GB',
+        time: '4.h'
+    ]
+}

From cf5802e4c323176296d1b09d9c9f1f4fa0054aa3 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 26 Aug 2025 12:44:19 +0200
Subject: [PATCH 793/887] update snap

---
 tests/test_stub.nf.test.snap | 42 ++++++------------------------------
 1 file changed, 6 insertions(+), 36 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 759d060d9..70a4596e5 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -507,20 +507,14 @@
     },
     "stub test fusioninspector": {
         "content": [
-            24,
+            21,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
                 },
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.1"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
@@ -582,10 +576,6 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0",
-                    "vcf_collect": 0.1
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -593,12 +583,9 @@
             [
                 "agat",
                 "agat/Homo_sapiens.GRCh38.46.gtf.bed",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "bioawk",
                 "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "fastqc",
@@ -768,16 +755,14 @@
                 "star/test.toTranscriptome.out.bam",
                 "star/test.unmapped_1.fastq.gz",
                 "star/test.unmapped_2.fastq.gz",
-                "star/testXd.out.bam",
-                "vcf",
-                "vcf/test_fusion_data.vcf"
+                "star/testXd.out.bam"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:16:11.496999226"
+        "timestamp": "2025-08-26T12:27:13.997309"
     },
     "stub test salmon": {
         "content": [
@@ -1600,20 +1585,14 @@
     },
     "stub test all": {
         "content": [
-            35,
+            32,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
                 },
-                "AGAT_CONVERTSPGFF2TSV": {
-                    "agat": "v1.4.1"
-                },
                 "ARRIBA_ARRIBA": {
                     "arriba": "2.5.0"
                 },
-                "ARRIBA_VISUALISATION": {
-                    "arriba": "2.5.0"
-                },
                 "BIOAWK": {
                     "bioawk": 1.0
                 },
@@ -1708,10 +1687,6 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
-                "VCF_COLLECT": {
-                    "python": "3.11.0",
-                    "vcf_collect": 0.1
-                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1719,12 +1694,9 @@
             [
                 "agat",
                 "agat/Homo_sapiens.GRCh38.46.gtf.bed",
-                "agat/test.tsv",
                 "arriba",
                 "arriba/test.arriba.fusions.discarded.tsv",
                 "arriba/test.arriba.fusions.tsv",
-                "arriba_visualisation",
-                "arriba_visualisation/test_combined_fusions_arriba_visualisation.pdf",
                 "bioawk",
                 "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
                 "ctatsplicing",
@@ -1956,15 +1928,13 @@
                 "stringtie/test.ballgown",
                 "stringtie/test.coverage.gtf",
                 "stringtie/test.gene.abundance.txt",
-                "stringtie/test.transcripts.gtf",
-                "vcf",
-                "vcf/test_fusion_data.vcf"
+                "stringtie/test.transcripts.gtf"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-18T16:02:59.744224309"
+        "timestamp": "2025-08-25T16:52:54.430967"
     }
 }
\ No newline at end of file

From ace5d0c2283b64b208ef2cbfbf20b6f75dc3a6a4 Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Tue, 26 Aug 2025 20:18:13 +0200
Subject: [PATCH 794/887] Update test

---
 conf/modules.config                           |  1 -
 .../local/fusioninspector_workflow/main.nf    |  8 ++-
 .../tests/main.nf.test                        | 64 ++++++++++++-----
 .../tests/main.nf.test.snap                   | 72 +++++++++++++++++++
 .../tests/nextflow.config                     | 20 ++----
 workflows/rnafusion.nf                        |  4 +-
 6 files changed, 132 insertions(+), 37 deletions(-)
 create mode 100644 subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap

diff --git a/conf/modules.config b/conf/modules.config
index 8e0ba6060..548cc1895 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -329,7 +329,6 @@ process {
         ext.args = "--max_readlength ${params.read_length} --human_gencode_filter"
     }
 
-
     withName: 'STRINGTIE_MERGE' {
         publishDir = [
             path: { "${params.outdir}/stringtie/${meta.id}" },
diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index ec6e4fafd..40257fa61 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -20,15 +20,17 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         ch_starfusion_ref
         skip_vis
         skip_vcf
+        tools_cutoff
+        whitelist
 
     main:
         ch_versions = Channel.empty()
         ch_arriba_visualisation = Channel.empty()
 
-        ch_fusion_list = ( params.tools_cutoff > 1 ? fusion_list_filtered : fusion_list )
+        ch_fusion_list = ( tools_cutoff > 1 ? fusion_list_filtered : fusion_list )
 
-        if (params.whitelist)  {
-            ch_whitelist = ch_fusion_list.combine(Channel.value(file(params.whitelist, checkIfExists:true)))
+        if (whitelist)  {
+            ch_whitelist = ch_fusion_list.combine(Channel.value(file(whitelist, checkIfExists:true)))
                             .map { meta, fusions, whitelist -> [ meta, [fusions, whitelist] ] }
 
             CAT_CAT(ch_whitelist) // fusioninspector takes care of possible duplicates
diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
index 0e3930b83..4483e8f86 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
@@ -5,7 +5,8 @@ nextflow_workflow {
     workflow "FUSIONINSPECTOR_WORKFLOW"
     config './nextflow.config'
 
-     test("FUSIONINSPECTOR_WORKFLOW - human") {
+    test("FUSIONINSPECTOR_WORKFLOW - human") {
+
         setup {
             run("STARFUSION_BUILD") {
                 script "../../../../modules/nf-core/starfusion/build/main.nf"
@@ -13,15 +14,14 @@ nextflow_workflow {
                     """
                     input[0] = [
                         [ id:'minigenome_fasta' ],
-                        file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46_dna_primary_assembly.fa")
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.fa')
                     ]
                     input[1] = [
                         [ id:'minigenome_gtf' ],
-                        file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf")
+                        file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.gtf')
                     ]
                     input[2] = file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                     input[3] = "homo_sapiens"
-                    input[3] = "homo_sapiens"
                     input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
                     input[5] = [
                         "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
@@ -61,6 +61,15 @@ nextflow_workflow {
         }
 
         when {
+            params {
+                no_cosmic                  = true
+                whitelist                  = []
+                outdir                     = "test"
+                genomes_base               = "test"
+                fusionreport_download_args = '--no-cosmic'
+                fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
+                fusionreport_detect_args2  = "--export csv"
+            }
             workflow {
                 """
                 input[0] = Channel.of(
@@ -70,21 +79,27 @@ nextflow_workflow {
                         file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
                     ]
                 ])
-                input[1] = FUSIONREPORT_DETECT.out.fusion_list
-                input[2] = FUSIONREPORT_DETECT.out.fusion_list_filtered
+                input[1] = Channel.of([ [id:'test_sample'], file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")])
+                input[2] = Channel.of([ [id:'test_sample'], file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")])
                 input[3] = FUSIONREPORT_DETECT.out.report
                 input[4] = FUSIONREPORT_DETECT.out.csv
-                input[5] = [ [], [], [] ]
+                input[5] = Channel.of([
+                    [id:'test_sample'],
+                    [],
+                    []
+                ])
                 input[6] = Channel.of(
                     [ [ id:'test_gtf' ],
-                    file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf")])
+                    file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.gtf')])
                 input[7] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"))
                 input[8] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"))
                 input[9] = Channel.of([[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")] )
                 input[10] = Channel.of([[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")] )
                 input[11] = STARFUSION_BUILD.out.reference
-                input[12] = true
-                input[13] = false
+                input[12] = false
+                input[13] = true
+                input[14] = 1
+                input[15] = params.whitelist
                 """
             }
         }
@@ -99,6 +114,7 @@ nextflow_workflow {
 
     test("FUSIONINSPECTOR_WORKFLOW - human - stub") {
         options "-stub"
+
         setup {
             run("STARFUSION_BUILD") {
                 script "../../../../modules/nf-core/starfusion/build/main.nf"
@@ -114,7 +130,6 @@ nextflow_workflow {
                     ]
                     input[2] = file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                     input[3] = "homo_sapiens"
-                    input[3] = "homo_sapiens"
                     input[4] = "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/Pfam-A.hmm.gz"
                     input[5] = [
                         "https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/human/test_starfusion_dfam.hmm",
@@ -154,6 +169,15 @@ nextflow_workflow {
         }
 
         when {
+            params {
+                no_cosmic                  = true
+                whitelist                  = []
+                outdir                     = "test"
+                genomes_base               = "test"
+                fusionreport_download_args = '--no-cosmic'
+                fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
+                fusionreport_detect_args2  = "--export csv"
+            }
             workflow {
                 """
                 input[0] = Channel.of(
@@ -163,21 +187,27 @@ nextflow_workflow {
                         file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/v0.0.1/rnaseq_2.fastq.gz")
                     ]
                 ])
-                input[1] = FUSIONREPORT_DETECT.out.fusion_list
-                input[2] = FUSIONREPORT_DETECT.out.fusion_list_filtered
+                input[1] = Channel.of([ [id:'test_sample'], file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")])
+                input[2] = Channel.of([ [id:'test_sample'], file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")])
                 input[3] = FUSIONREPORT_DETECT.out.report
                 input[4] = FUSIONREPORT_DETECT.out.csv
-                input[5] = [ [], [], [] ]
+                input[5] = Channel.of([
+                    [id:'test_sample'],
+                    [],
+                    []
+                ])
                 input[6] = Channel.of(
                     [ [ id:'test_gtf' ],
-                    file("s3://nf-core-awsmegatests/rnafusion/references/GRCh38/gencode_v46/gencode/Homo_sapiens_GRCh38_46.gtf")])
+                    file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.gtf')])
                 input[7] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"))
                 input[8] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"))
                 input[9] = Channel.of([[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")] )
                 input[10] = Channel.of([[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")] )
                 input[11] = STARFUSION_BUILD.out.reference
-                input[12] = true
-                input[13] = false
+                input[12] = false
+                input[13] = true
+                input[14] = 1
+                input[15] = params.whitelist
                 """
             }
         }
diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
new file mode 100644
index 000000000..7677e5b5f
--- /dev/null
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
@@ -0,0 +1,72 @@
+{
+    "FUSIONINSPECTOR_WORKFLOW - human - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
+                    "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
+                ],
+                "ch_arriba_visualisation": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
+                    "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
+        },
+        "timestamp": "2025-08-26T20:11:13.798144726"
+    },
+    "FUSIONINSPECTOR_WORKFLOW - human": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,8264fb7e5cbf6aab0b7971953ddd9b87"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
+                    "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
+                ],
+                "ch_arriba_visualisation": [
+                    [
+                        {
+                            "id": "test_sample"
+                        },
+                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,8264fb7e5cbf6aab0b7971953ddd9b87"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
+                    "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.2"
+        },
+        "timestamp": "2025-08-26T20:05:51.190155915"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
index 25a00a186..50818cf99 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
+++ b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
@@ -1,18 +1,8 @@
-params {
-    arriba                     = true
-    starfusion                 = true
-    fusioncatcher              = true
-    no_cosmic                  = true
-    tools_cutoff               = 1
-    whitelist                  = null
-    outdir                     = "test"
-    genomes_base               = "test"
-    fusionreport_download_args = '--no-cosmic'
-    fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
-    fusionreport_detect_args2  = "--export csv"
-}
-
 process {
+    withName: 'STARFUSION_BUILD' {
+        ext.args = []
+    }
+
     withName: 'FUSIONREPORT_DOWNLOAD' {
         ext.args         =  params.fusionreport_download_args
     }
@@ -31,4 +21,4 @@ process {
         memory: '36.GB',
         time: '4.h'
     ]
-}
+}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 06a5dd26c..ece00c82b 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -357,7 +357,9 @@ workflow RNAFUSION {
                 BUILD_REFERENCES.out.hgnc_date,
                 BUILD_REFERENCES.out.starfusion_ref,
                 params.skip_vis,
-                params.skip_vcf
+                params.skip_vcf,
+                params.tools_cutoff,
+                params.whitelist
             )
             ch_versions      = ch_versions.mix(FUSIONINSPECTOR_WORKFLOW.out.versions)
             ch_multiqc_files = ch_multiqc_files.mix(FUSIONINSPECTOR_WORKFLOW.out.ch_arriba_visualisation.collect{it[1]}.ifEmpty([]))

From 8822e21322141e67f20cd851097c55102064fb90 Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Tue, 26 Aug 2025 20:20:11 +0200
Subject: [PATCH 795/887] Update gitignore

---
 .gitignore | 3 +++
 1 file changed, 3 insertions(+)

diff --git a/.gitignore b/.gitignore
index 23b0c7de9..f3ddfe03c 100644
--- a/.gitignore
+++ b/.gitignore
@@ -8,3 +8,6 @@ testing*
 *.pyc
 null/
 .nf-test*
+log/
+test/
+reports/

From e8b1eeb09537f35a827dd72d48c378a35eaf7350 Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Tue, 26 Aug 2025 20:59:48 +0200
Subject: [PATCH 796/887] Update tags

---
 .../local/fusioninspector_workflow/tests/main.nf.test | 11 +++++++++++
 1 file changed, 11 insertions(+)

diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
index 4483e8f86..cc579b57e 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
@@ -4,6 +4,17 @@ nextflow_workflow {
     script "../main.nf"
     workflow "FUSIONINSPECTOR_WORKFLOW"
     config './nextflow.config'
+    tag 'subworkflows'
+    tag 'subworkflows/fusioninspector_workflow'
+    tag 'cat/cat'
+    tag 'subworkflows_nfcore'
+    tag 'fusionreport/detect'
+    tag 'fusioninspector'
+    tag 'starfusion/build'
+    tag 'fusionreport/download'
+    tag 'arriba/visualisation'
+    tag 'subworkflows/../../modules/local/vcf_collect'
+    tag 'agat/convertspgff2tsv'
 
     test("FUSIONINSPECTOR_WORKFLOW - human") {
 

From e756a33dc836ab7f098dd2cce84ddae2e3678713 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Tue, 26 Aug 2025 16:00:48 -0300
Subject: [PATCH 797/887] Update .gitignore

---
 .gitignore | 3 ---
 1 file changed, 3 deletions(-)

diff --git a/.gitignore b/.gitignore
index f3ddfe03c..23b0c7de9 100644
--- a/.gitignore
+++ b/.gitignore
@@ -8,6 +8,3 @@ testing*
 *.pyc
 null/
 .nf-test*
-log/
-test/
-reports/

From c2cad26cfd18ce1807a0f6b26d87274e3927dabe Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Tue, 26 Aug 2025 16:08:57 -0300
Subject: [PATCH 798/887] Update nextflow.config

---
 .../local/fusioninspector_workflow/tests/nextflow.config        | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
index 50818cf99..808fbdffa 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
+++ b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
@@ -21,4 +21,4 @@ process {
         memory: '36.GB',
         time: '4.h'
     ]
-}
\ No newline at end of file
+}

From e839eab60eb68ac5c19692c3b54ba08c665cdfda Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Tue, 26 Aug 2025 16:58:43 -0300
Subject: [PATCH 799/887] Update nextflow.config

---
 .../local/fusioninspector_workflow/tests/nextflow.config    | 6 ------
 1 file changed, 6 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
index 808fbdffa..e00f3ced2 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
+++ b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
@@ -15,10 +15,4 @@ process {
     withName: 'FUSIONINSPECTOR' {
         ext.args = '--annotate --examine_coding_effect'
     }
-
-    resourceLimits = [
-        cpus: 6,
-        memory: '36.GB',
-        time: '4.h'
-    ]
 }

From f8a579084b63172af61e96b864ba88c8a7c447b8 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Tue, 26 Aug 2025 20:21:33 +0000
Subject: [PATCH 800/887] Change to nf-core subworkflow of `fastq_align_star`

---
 conf/modules.config                           |   5 +
 modules.json                                  |  36 +-
 .../nf-core/samtools/flagstat/environment.yml |   8 +
 modules/nf-core/samtools/flagstat/main.nf     |  45 +++
 modules/nf-core/samtools/flagstat/meta.yml    |  60 +++
 .../samtools/flagstat/tests/main.nf.test      |  56 +++
 .../samtools/flagstat/tests/main.nf.test.snap |  72 ++++
 .../nf-core/samtools/idxstats/environment.yml |   8 +
 modules/nf-core/samtools/idxstats/main.nf     |  48 +++
 modules/nf-core/samtools/idxstats/meta.yml    |  60 +++
 .../samtools/idxstats/tests/main.nf.test      |  53 +++
 .../samtools/idxstats/tests/main.nf.test.snap |  72 ++++
 .../nf-core/samtools/stats/environment.yml    |   8 +
 modules/nf-core/samtools/stats/main.nf        |  48 +++
 modules/nf-core/samtools/stats/meta.yml       |  72 ++++
 .../nf-core/samtools/stats/tests/main.nf.test | 113 ++++++
 .../samtools/stats/tests/main.nf.test.snap    | 142 +++++++
 subworkflows/local/fastq_align_star/main.nf   |  55 ---
 .../nf-core/bam_sort_stats_samtools/main.nf   |  50 +++
 .../nf-core/bam_sort_stats_samtools/meta.yml  |  70 ++++
 .../tests/main.nf.test                        | 134 +++++++
 .../tests/main.nf.test.snap                   | 330 +++++++++++++++++
 .../nf-core/bam_stats_samtools/main.nf        |  32 ++
 .../nf-core/bam_stats_samtools/meta.yml       |  43 +++
 .../bam_stats_samtools/tests/main.nf.test     | 188 ++++++++++
 .../tests/main.nf.test.snap                   | 350 ++++++++++++++++++
 subworkflows/nf-core/fastq_align_star/main.nf |  69 ++++
 .../nf-core/fastq_align_star/meta.yml         | 133 +++++++
 .../nf-core/fastq_align_star/nextflow.config  |  10 +
 .../fastq_align_star/tests/main.nf.test       | 250 +++++++++++++
 .../fastq_align_star/tests/main.nf.test.snap  | 282 ++++++++++++++
 .../fastq_align_star/tests/nextflow.config    |  21 ++
 .../tests/with_transcripts.config             |  21 ++
 workflows/rnafusion.nf                        |  16 +-
 34 files changed, 2897 insertions(+), 63 deletions(-)
 create mode 100644 modules/nf-core/samtools/flagstat/environment.yml
 create mode 100644 modules/nf-core/samtools/flagstat/main.nf
 create mode 100644 modules/nf-core/samtools/flagstat/meta.yml
 create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/idxstats/environment.yml
 create mode 100644 modules/nf-core/samtools/idxstats/main.nf
 create mode 100644 modules/nf-core/samtools/idxstats/meta.yml
 create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
 create mode 100644 modules/nf-core/samtools/stats/environment.yml
 create mode 100644 modules/nf-core/samtools/stats/main.nf
 create mode 100644 modules/nf-core/samtools/stats/meta.yml
 create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test
 create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test.snap
 delete mode 100644 subworkflows/local/fastq_align_star/main.nf
 create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/main.nf
 create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/meta.yml
 create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/main.nf
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/meta.yml
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/fastq_align_star/main.nf
 create mode 100644 subworkflows/nf-core/fastq_align_star/meta.yml
 create mode 100644 subworkflows/nf-core/fastq_align_star/nextflow.config
 create mode 100644 subworkflows/nf-core/fastq_align_star/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/fastq_align_star/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/fastq_align_star/tests/nextflow.config
 create mode 100644 subworkflows/nf-core/fastq_align_star/tests/with_transcripts.config

diff --git a/conf/modules.config b/conf/modules.config
index 8e0ba6060..e40b81b54 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -261,6 +261,11 @@ process {
         ]
     }
 
+    withName: SAMTOOLS_SORT {
+        ext.prefix  = { "${meta.id}.Aligned.sortedByCoord" }
+        ext.args    = { params.cram ? "--write-index --output-fmt cram" : "--write-index" }
+    }
+
     withName: 'STAR_ALIGN' {
         publishDir = [
             path: { "${params.outdir}/star" },
diff --git a/modules.json b/modules.json
index 61e76f0a3..463d52bd1 100644
--- a/modules.json
+++ b/modules.json
@@ -142,20 +142,35 @@
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["modules"]
                     },
+                    "samtools/flagstat": {
+                        "branch": "master",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "installed_by": ["bam_stats_samtools"]
+                    },
+                    "samtools/idxstats": {
+                        "branch": "master",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "installed_by": ["bam_stats_samtools"]
+                    },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
+                    },
+                    "samtools/stats": {
+                        "branch": "master",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": ["fastq_align_star", "modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
@@ -191,6 +206,21 @@
             },
             "subworkflows": {
                 "nf-core": {
+                    "bam_sort_stats_samtools": {
+                        "branch": "master",
+                        "git_sha": "e89ff68a46b848c4d8aabb8569767516d46db319",
+                        "installed_by": ["fastq_align_star"]
+                    },
+                    "bam_stats_samtools": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": ["bam_sort_stats_samtools"]
+                    },
+                    "fastq_align_star": {
+                        "branch": "master",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": ["subworkflows"]
+                    },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
diff --git a/modules/nf-core/samtools/flagstat/environment.yml b/modules/nf-core/samtools/flagstat/environment.yml
new file mode 100644
index 000000000..62054fc97
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf
new file mode 100644
index 000000000..c23f3a5cf
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/main.nf
@@ -0,0 +1,45 @@
+process SAMTOOLS_FLAGSTAT {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path("*.flagstat"), emit: flagstat
+    path  "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    samtools \\
+        flagstat \\
+        --threads ${task.cpus} \\
+        $bam \\
+        > ${prefix}.flagstat
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.flagstat
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/samtools/flagstat/meta.yml b/modules/nf-core/samtools/flagstat/meta.yml
new file mode 100644
index 000000000..ebbc15f27
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/meta.yml
@@ -0,0 +1,60 @@
+name: samtools_flagstat
+description: Counts the number of alignments in a BAM/CRAM/SAM file for each FLAG
+  type
+keywords:
+  - stats
+  - mapping
+  - counts
+  - bam
+  - sam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+      identifier: biotools:samtools
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+        ontologies: []
+    - bai:
+        type: file
+        description: Index for BAM/CRAM/SAM file
+        pattern: "*.{bai,crai,sai}"
+        ontologies: []
+output:
+  flagstat:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.flagstat":
+          type: file
+          description: File containing samtools flagstat output
+          pattern: "*.{flagstat}"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test b/modules/nf-core/samtools/flagstat/tests/main.nf.test
new file mode 100644
index 000000000..3b648a37d
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test
@@ -0,0 +1,56 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_FLAGSTAT"
+    script "../main.nf"
+    process "SAMTOOLS_FLAGSTAT"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/flagstat"
+
+    test("BAM") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("BAM - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
new file mode 100644
index 000000000..04c3852b2
--- /dev/null
+++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap
@@ -0,0 +1,72 @@
+{
+    "BAM - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:02:58.866491759"
+    },
+    "BAM": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,108a155f2d4a99f50bf3176904208d27"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:02:47.383332837"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/idxstats/environment.yml b/modules/nf-core/samtools/idxstats/environment.yml
new file mode 100644
index 000000000..62054fc97
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf
new file mode 100644
index 000000000..3d5bf4bdc
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/main.nf
@@ -0,0 +1,48 @@
+process SAMTOOLS_IDXSTATS {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+
+    input:
+    tuple val(meta), path(bam), path(bai)
+
+    output:
+    tuple val(meta), path("*.idxstats"), emit: idxstats
+    path  "versions.yml"               , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    # Note: --threads value represents *additional* CPUs to allocate (total CPUs = 1 + --threads).
+    samtools \\
+        idxstats \\
+        --threads ${task.cpus-1} \\
+        $bam \\
+        > ${prefix}.idxstats
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    """
+    touch ${prefix}.idxstats
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/samtools/idxstats/meta.yml b/modules/nf-core/samtools/idxstats/meta.yml
new file mode 100644
index 000000000..96d427466
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/meta.yml
@@ -0,0 +1,60 @@
+name: samtools_idxstats
+description: Reports alignment summary statistics for a BAM/CRAM/SAM file
+keywords:
+  - stats
+  - mapping
+  - counts
+  - chromosome
+  - bam
+  - sam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+      identifier: biotools:samtools
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bam:
+        type: file
+        description: BAM/CRAM/SAM file
+        pattern: "*.{bam,cram,sam}"
+        ontologies: []
+    - bai:
+        type: file
+        description: Index for BAM/CRAM/SAM file
+        pattern: "*.{bai,crai,sai}"
+        ontologies: []
+output:
+  idxstats:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.idxstats":
+          type: file
+          description: File containing samtools idxstats output
+          pattern: "*.{idxstats}"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test b/modules/nf-core/samtools/idxstats/tests/main.nf.test
new file mode 100644
index 000000000..5fd1fc78e
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test
@@ -0,0 +1,53 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_IDXSTATS"
+    script "../main.nf"
+    process "SAMTOOLS_IDXSTATS"
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/idxstats"
+
+    test("bam") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
+    test("bam - stub") {
+        options "-stub"
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }}
diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
new file mode 100644
index 000000000..2cc89a3b8
--- /dev/null
+++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap
@@ -0,0 +1,72 @@
+{
+    "bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:11:56.466856235"
+    },
+    "bam": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,c8d7394830c3c1e5be150589571534fb"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:11:46.311550359"
+    }
+}
\ No newline at end of file
diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml
new file mode 100644
index 000000000..62054fc97
--- /dev/null
+++ b/modules/nf-core/samtools/stats/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf
new file mode 100644
index 000000000..4443948b7
--- /dev/null
+++ b/modules/nf-core/samtools/stats/main.nf
@@ -0,0 +1,48 @@
+process SAMTOOLS_STATS {
+    tag "$meta.id"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
+        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+
+    input:
+    tuple val(meta), path(input), path(input_index)
+    tuple val(meta2), path(fasta)
+
+    output:
+    tuple val(meta), path("*.stats"), emit: stats
+    path  "versions.yml"            , emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def reference = fasta ? "--reference ${fasta}" : ""
+    """
+    samtools \\
+        stats \\
+        --threads ${task.cpus} \\
+        ${reference} \\
+        ${input} \\
+        > ${prefix}.stats
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.stats
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
+    END_VERSIONS
+    """
+}
diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml
new file mode 100644
index 000000000..6dc51885d
--- /dev/null
+++ b/modules/nf-core/samtools/stats/meta.yml
@@ -0,0 +1,72 @@
+name: samtools_stats
+description: Produces comprehensive statistics from SAM/BAM/CRAM file
+keywords:
+  - statistics
+  - counts
+  - bam
+  - sam
+  - cram
+tools:
+  - samtools:
+      description: |
+        SAMtools is a set of utilities for interacting with and post-processing
+        short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
+        These files are generated as output by short read aligners like BWA.
+      homepage: http://www.htslib.org/
+      documentation: http://www.htslib.org/doc/samtools.html
+      doi: 10.1093/bioinformatics/btp352
+      licence: ["MIT"]
+      identifier: biotools:samtools
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - input:
+        type: file
+        description: BAM/CRAM file from alignment
+        pattern: "*.{bam,cram}"
+        ontologies: []
+    - input_index:
+        type: file
+        description: BAI/CRAI file from alignment
+        pattern: "*.{bai,crai}"
+        ontologies: []
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: Reference file the CRAM was created with (optional)
+        pattern: "*.{fasta,fa}"
+        ontologies: []
+output:
+  stats:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.stats":
+          type: file
+          description: File containing samtools stats output
+          pattern: "*.{stats}"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+authors:
+  - "@drpatelh"
+  - "@FriederikeHanssen"
+  - "@ramprasadn"
+maintainers:
+  - "@drpatelh"
+  - "@FriederikeHanssen"
+  - "@ramprasadn"
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test
new file mode 100644
index 000000000..5bc893095
--- /dev/null
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test
@@ -0,0 +1,113 @@
+nextflow_process {
+
+    name "Test Process SAMTOOLS_STATS"
+    script "../main.nf"
+    process "SAMTOOLS_STATS"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "samtools"
+    tag "samtools/stats"
+
+    test("bam") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+
+    test("cram") {
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+
+    test("bam - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = [[],[]]
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+
+    test("cram - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                {assert process.success},
+                {assert snapshot(process.out).match()}
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
new file mode 100644
index 000000000..df507be7a
--- /dev/null
+++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap
@@ -0,0 +1,142 @@
+{
+    "cram": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,a27fe55e49a341f92379bb20a65c6a06"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,a27fe55e49a341f92379bb20a65c6a06"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:29:16.767396182"
+    },
+    "bam - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:29:29.721580274"
+    },
+    "cram - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:29:53.567964304"
+    },
+    "bam": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d53a2584376d78942839e9933a34d11b"
+                    ]
+                ],
+                "1": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d53a2584376d78942839e9933a34d11b"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,15b91d8c0e0440332e0fe4df80957043"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T09:28:50.73610604"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/local/fastq_align_star/main.nf b/subworkflows/local/fastq_align_star/main.nf
deleted file mode 100644
index fe9848c39..000000000
--- a/subworkflows/local/fastq_align_star/main.nf
+++ /dev/null
@@ -1,55 +0,0 @@
-include { SAMTOOLS_CONVERT  } from '../../../modules/nf-core/samtools/convert/main'
-include { STAR_ALIGN        } from '../../../modules/nf-core/star/align/main'
-include { SAMTOOLS_INDEX    } from '../../../modules/nf-core/samtools/index/main.nf'
-
-workflow FASTQ_ALIGN_STAR {
-    take:
-    reads           // channel: [ meta, [ fastq1, fastq2 ]]
-    index           // channel: [ meta, index ]
-    gtf             // channel: [ meta, gtf ]
-    fasta           // channel: [ meta, fasta ]
-    fai             // channel: [ meta, fai ]
-    ignore_sjdbgtf  // boolean
-    cram            // boolean: Create CRAM files
-
-    main:
-    def ch_versions = Channel.empty()
-    STAR_ALIGN(
-        reads,
-        index,
-        gtf,
-        ignore_sjdbgtf,
-        "", // seq_platform is handled in the config
-        ""  // seq_center is handled in the config
-    )
-    ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first())
-
-    SAMTOOLS_INDEX(
-        STAR_ALIGN.out.bam_sorted_aligned
-    )
-    ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first())
-
-    ch_bam_bai = STAR_ALIGN.out.bam_sorted_aligned
-        .join(SAMTOOLS_INDEX.out.bai, failOnMismatch:true, failOnDuplicate:true)
-
-    def ch_cram_crai = Channel.empty()
-    if(cram) {
-        SAMTOOLS_CONVERT(
-            STAR_ALIGN.out.bam_sorted_aligned.map { meta, bam -> [ meta, bam, []] },
-            fasta,
-            fai
-        )
-        ch_versions = ch_versions.mix(SAMTOOLS_CONVERT.out.versions.first())
-        ch_cram_crai = SAMTOOLS_CONVERT.out.cram
-            .join(SAMTOOLS_CONVERT.out.crai, failOnMismatch:true, failOnDuplicate:true)
-    }
-
-    emit:
-    versions      = ch_versions              // channel [ versions ]
-    bam_bai       = ch_bam_bai
-    cram_crai     = ch_cram_crai
-    junctions     = STAR_ALIGN.out.junction
-    spl_junc_tabs = STAR_ALIGN.out.spl_junc_tab
-    log_final     = STAR_ALIGN.out.log_final
-    gene_count    = STAR_ALIGN.out.read_per_gene_tab
-}
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
new file mode 100644
index 000000000..460726368
--- /dev/null
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
@@ -0,0 +1,50 @@
+//
+// Sort, index BAM file and run samtools stats, flagstat and idxstats
+//
+
+include { SAMTOOLS_SORT      } from '../../../modules/nf-core/samtools/sort/main'
+include { SAMTOOLS_INDEX     } from '../../../modules/nf-core/samtools/index/main'
+include { BAM_STATS_SAMTOOLS } from '../bam_stats_samtools/main'
+
+workflow BAM_SORT_STATS_SAMTOOLS {
+    take:
+    ch_bam   // channel: [ val(meta), [ bam ] ]
+    ch_fasta // channel: [ val(meta), path(fasta) ]
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    SAMTOOLS_SORT ( ch_bam, ch_fasta, '' )
+    ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first())
+
+    SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam )
+    ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first())
+
+    SAMTOOLS_SORT.out.bam
+        .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true)
+        .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true)
+        .map {
+            meta, bam, bai, csi ->
+                if (bai) {
+                    [ meta, bam, bai ]
+                } else {
+                    [ meta, bam, csi ]
+                }
+        }
+        .set { ch_bam_bai }
+
+    BAM_STATS_SAMTOOLS ( ch_bam_bai, ch_fasta )
+    ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions)
+
+    emit:
+    bam      = SAMTOOLS_SORT.out.bam           // channel: [ val(meta), [ bam ] ]
+    bai      = SAMTOOLS_INDEX.out.bai          // channel: [ val(meta), [ bai ] ]
+    csi      = SAMTOOLS_INDEX.out.csi          // channel: [ val(meta), [ csi ] ]
+
+    stats    = BAM_STATS_SAMTOOLS.out.stats    // channel: [ val(meta), [ stats ] ]
+    flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ]
+    idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ]
+
+    versions = ch_versions                     // channel: [ versions.yml ]
+}
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml
new file mode 100644
index 000000000..e01f9ccf6
--- /dev/null
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml
@@ -0,0 +1,70 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: bam_sort_stats_samtools
+description: Sort SAM/BAM/CRAM file
+keywords:
+  - sort
+  - bam
+  - sam
+  - cram
+components:
+  - samtools/sort
+  - samtools/index
+  - samtools/stats
+  - samtools/idxstats
+  - samtools/flagstat
+  - bam_stats_samtools
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - fasta:
+      type: file
+      description: Reference genome fasta file
+      pattern: "*.{fasta,fa}"
+# TODO Update when we decide on a standard for subworkflow docs
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - bam:
+      type: file
+      description: Sorted BAM/CRAM/SAM file
+      pattern: "*.{bam,cram,sam}"
+  - bai:
+      type: file
+      description: BAM/CRAM/SAM index file
+      pattern: "*.{bai,crai,sai}"
+  - crai:
+      type: file
+      description: BAM/CRAM/SAM index file
+      pattern: "*.{bai,crai,sai}"
+  - stats:
+      type: file
+      description: File containing samtools stats output
+      pattern: "*.{stats}"
+  - flagstat:
+      type: file
+      description: File containing samtools flagstat output
+      pattern: "*.{flagstat}"
+  - idxstats:
+      type: file
+      description: File containing samtools idxstats output
+      pattern: "*.{idxstats}"
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@drpatelh"
+  - "@ewels"
+maintainers:
+  - "@drpatelh"
+  - "@ewels"
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
new file mode 100644
index 000000000..821a3cf50
--- /dev/null
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test
@@ -0,0 +1,134 @@
+nextflow_workflow {
+
+    name "Test Workflow BAM_SORT_STATS_SAMTOOLS"
+    script "../main.nf"
+    workflow "BAM_SORT_STATS_SAMTOOLS"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "subworkflows/bam_sort_stats_samtools"
+    tag "bam_sort_stats_samtools"
+    tag "subworkflows/bam_stats_samtools"
+    tag "bam_stats_samtools"
+    tag "samtools"
+    tag "samtools/index"
+    tag "samtools/sort"
+    tag "samtools/stats"
+    tag "samtools/idxstats"
+    tag "samtools/flagstat"
+
+    test("test_bam_sort_stats_samtools_single_end") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert workflow.out.bam.get(0).get(1) ==~ ".*.bam"},
+                { assert workflow.out.bai.get(0).get(1) ==~ ".*.bai"},
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_bam_sort_stats_samtools_paired_end") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert workflow.out.bam.get(0).get(1) ==~ ".*.bam"},
+                { assert workflow.out.bai.get(0).get(1) ==~ ".*.bai"},
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_bam_sort_stats_samtools_single_end - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("test_bam_sort_stats_samtools_paired_end - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
new file mode 100644
index 000000000..e4b8af9a5
--- /dev/null
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap
@@ -0,0 +1,330 @@
+{
+    "test_bam_sort_stats_samtools_single_end": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.stats:md5,2fe0f3a7a1f07906061c1dadb62e0d05"
+                ]
+            ],
+            [
+                "versions.yml:md5,032c89015461d597fcc5a5331b619d0a",
+                "versions.yml:md5,416c5e4a374c61167db999b0e400e3cf",
+                "versions.yml:md5,721391fd94c417808516480c9451c6fd",
+                "versions.yml:md5,9e12386b91a2977d23292754e3bcb522",
+                "versions.yml:md5,c294c162aeb09862cc5e55b602647452"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T14:07:28.387184086"
+    },
+    "test_bam_sort_stats_samtools_paired_end": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.stats:md5,ba007b13981dad548358c7c957d41e12"
+                ]
+            ],
+            [
+                "versions.yml:md5,032c89015461d597fcc5a5331b619d0a",
+                "versions.yml:md5,416c5e4a374c61167db999b0e400e3cf",
+                "versions.yml:md5,721391fd94c417808516480c9451c6fd",
+                "versions.yml:md5,9e12386b91a2977d23292754e3bcb522",
+                "versions.yml:md5,c294c162aeb09862cc5e55b602647452"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T14:07:39.841563832"
+    },
+    "test_bam_sort_stats_samtools_single_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    "versions.yml:md5,032c89015461d597fcc5a5331b619d0a",
+                    "versions.yml:md5,416c5e4a374c61167db999b0e400e3cf",
+                    "versions.yml:md5,721391fd94c417808516480c9451c6fd",
+                    "versions.yml:md5,9e12386b91a2977d23292754e3bcb522",
+                    "versions.yml:md5,c294c162aeb09862cc5e55b602647452"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,032c89015461d597fcc5a5331b619d0a",
+                    "versions.yml:md5,416c5e4a374c61167db999b0e400e3cf",
+                    "versions.yml:md5,721391fd94c417808516480c9451c6fd",
+                    "versions.yml:md5,9e12386b91a2977d23292754e3bcb522",
+                    "versions.yml:md5,c294c162aeb09862cc5e55b602647452"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T16:43:39.764967263"
+    },
+    "test_bam_sort_stats_samtools_paired_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    
+                ],
+                "3": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "4": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "5": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "6": [
+                    "versions.yml:md5,032c89015461d597fcc5a5331b619d0a",
+                    "versions.yml:md5,416c5e4a374c61167db999b0e400e3cf",
+                    "versions.yml:md5,721391fd94c417808516480c9451c6fd",
+                    "versions.yml:md5,9e12386b91a2977d23292754e3bcb522",
+                    "versions.yml:md5,c294c162aeb09862cc5e55b602647452"
+                ],
+                "bai": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam.bai:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "bam": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "csi": [
+                    
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,032c89015461d597fcc5a5331b619d0a",
+                    "versions.yml:md5,416c5e4a374c61167db999b0e400e3cf",
+                    "versions.yml:md5,721391fd94c417808516480c9451c6fd",
+                    "versions.yml:md5,9e12386b91a2977d23292754e3bcb522",
+                    "versions.yml:md5,c294c162aeb09862cc5e55b602647452"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T16:43:47.431619557"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/bam_stats_samtools/main.nf b/subworkflows/nf-core/bam_stats_samtools/main.nf
new file mode 100644
index 000000000..44d4c010a
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/main.nf
@@ -0,0 +1,32 @@
+//
+// Run SAMtools stats, flagstat and idxstats
+//
+
+include { SAMTOOLS_STATS    } from '../../../modules/nf-core/samtools/stats/main'
+include { SAMTOOLS_IDXSTATS } from '../../../modules/nf-core/samtools/idxstats/main'
+include { SAMTOOLS_FLAGSTAT } from '../../../modules/nf-core/samtools/flagstat/main'
+
+workflow BAM_STATS_SAMTOOLS {
+    take:
+    ch_bam_bai // channel: [ val(meta), path(bam), path(bai) ]
+    ch_fasta   // channel: [ val(meta), path(fasta) ]
+
+    main:
+    ch_versions = Channel.empty()
+
+    SAMTOOLS_STATS ( ch_bam_bai, ch_fasta )
+    ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions)
+
+    SAMTOOLS_FLAGSTAT ( ch_bam_bai )
+    ch_versions = ch_versions.mix(SAMTOOLS_FLAGSTAT.out.versions)
+
+    SAMTOOLS_IDXSTATS ( ch_bam_bai )
+    ch_versions = ch_versions.mix(SAMTOOLS_IDXSTATS.out.versions)
+
+    emit:
+    stats    = SAMTOOLS_STATS.out.stats       // channel: [ val(meta), path(stats) ]
+    flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), path(flagstat) ]
+    idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), path(idxstats) ]
+
+    versions = ch_versions                    // channel: [ path(versions.yml) ]
+}
diff --git a/subworkflows/nf-core/bam_stats_samtools/meta.yml b/subworkflows/nf-core/bam_stats_samtools/meta.yml
new file mode 100644
index 000000000..809bf736b
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/meta.yml
@@ -0,0 +1,43 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: bam_stats_samtools
+description: Produces comprehensive statistics from SAM/BAM/CRAM file
+keywords:
+  - statistics
+  - counts
+  - bam
+  - sam
+  - cram
+components:
+  - samtools/stats
+  - samtools/idxstats
+  - samtools/flagstat
+input:
+  - ch_bam_bai:
+      description: |
+        The input channel containing the BAM/CRAM and it's index
+        Structure: [ val(meta), path(bam), path(bai) ]
+  - ch_fasta:
+      description: |
+        Reference genome fasta file
+        Structure: [ path(fasta) ]
+output:
+  - stats:
+      description: |
+        File containing samtools stats output
+        Structure: [ val(meta), path(stats) ]
+  - flagstat:
+      description: |
+        File containing samtools flagstat output
+        Structure: [ val(meta), path(flagstat) ]
+  - idxstats:
+      description: |
+        File containing samtools idxstats output
+        Structure: [ val(meta), path(idxstats)]
+  - versions:
+      description: |
+        Files containing software versions
+        Structure: [ path(versions.yml) ]
+authors:
+  - "@drpatelh"
+maintainers:
+  - "@drpatelh"
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
new file mode 100644
index 000000000..76e7a40a0
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test
@@ -0,0 +1,188 @@
+nextflow_workflow {
+
+    name "Test Workflow BAM_STATS_SAMTOOLS"
+    script "../main.nf"
+    workflow "BAM_STATS_SAMTOOLS"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "bam_stats_samtools"
+    tag "subworkflows/bam_stats_samtools"
+    tag "samtools"
+    tag "samtools/flagstat"
+    tag "samtools/idxstats"
+    tag "samtools/stats"
+
+    test("test_bam_stats_samtools_single_end") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end_cram") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats,
+                    workflow.out.versions).match() }
+            )
+        }
+    }
+
+    test ("test_bam_stats_samtools_single_end - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+
+    test("test_bam_stats_samtools_paired_end_cram - stub") {
+
+        options "-stub"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'genome' ],
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
new file mode 100644
index 000000000..8ca225263
--- /dev/null
+++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap
@@ -0,0 +1,350 @@
+{
+    "test_bam_stats_samtools_paired_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:35.660286921"
+    },
+    "test_bam_stats_samtools_single_end - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": true
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:24.220305512"
+    },
+    "test_bam_stats_samtools_paired_end_cram - stub": {
+        "content": [
+            {
+                "0": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "1": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "2": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "3": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ],
+                "flagstat": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.flagstat:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "idxstats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.idxstats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "stats": [
+                    [
+                        {
+                            "id": "test",
+                            "single_end": false
+                        },
+                        "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions": [
+                    "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                    "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                    "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:54.206770141"
+    },
+    "test_bam_stats_samtools_single_end": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.stats:md5,291bb2393ec947140d12d42c2795b222"
+                ]
+            ],
+            [
+                "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:07:49.731645858"
+    },
+    "test_bam_stats_samtools_paired_end": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.stats:md5,8140d69cdedd77570ca1d7618a744e16"
+                ]
+            ],
+            [
+                "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:01.421996172"
+    },
+    "test_bam_stats_samtools_paired_end_cram": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.flagstat:md5,a53f3d26e2e9851f7d528442bbfe9781"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.idxstats:md5,e179601fa7b8ebce81ac3765206f6c15"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.stats:md5,1622856127bafd6cdbadee9cd64ec9b7"
+                ]
+            ],
+            [
+                "versions.yml:md5,73c55059ed478cd2f9cd93dd3185da3a",
+                "versions.yml:md5,80d8653e01575b3c381d87073f672fb5",
+                "versions.yml:md5,cb889532237a2f3d813978ac14a12d51"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-09-16T08:08:12.640915756"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/fastq_align_star/main.nf b/subworkflows/nf-core/fastq_align_star/main.nf
new file mode 100644
index 000000000..4b3602982
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/main.nf
@@ -0,0 +1,69 @@
+include { STAR_ALIGN                                                       } from '../../../modules/nf-core/star/align/main'
+include { BAM_SORT_STATS_SAMTOOLS as BAM_SORT_STATS_SAMTOOLS_GENOME        } from '../bam_sort_stats_samtools/main'
+include { BAM_SORT_STATS_SAMTOOLS as BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME } from '../bam_sort_stats_samtools/main'
+
+
+workflow FASTQ_ALIGN_STAR {
+
+    take:
+    ch_reads                    // channel: [ val(meta), [ path(reads) ] ]
+    ch_index                    // channel: [ path(index) ]
+    ch_gtf                      // channel: [ path(gtf) ]
+    val_star_ignore_sjdbgtf     // boolean: when using pre-built STAR indices do not re-extract and use splice junctions from the GTF file
+    val_seq_platform            // string : sequencing platform
+    val_seq_center              // string : sequencing center
+    ch_fasta                    // channel: [ val(meta), path(fasta) ]
+    ch_transcripts_fasta        // channel: [ val(meta), path(fasta) ]
+
+    main:
+
+    ch_versions = Channel.empty()
+
+    //
+    // Map reads with STAR
+    //
+    STAR_ALIGN ( ch_reads, ch_index, ch_gtf, val_star_ignore_sjdbgtf, val_seq_platform, val_seq_center )
+    ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first())
+
+    //
+    // Sort, index BAM file and run samtools stats, flagstat and idxstats
+    //
+    BAM_SORT_STATS_SAMTOOLS_GENOME ( STAR_ALIGN.out.bam, ch_fasta )
+    ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS_GENOME.out.versions)
+
+    //
+    // Sort, index BAM file and run samtools stats, flagstat and idxstats
+    //
+    // Only runs when '--quantMode TranscriptomeSAM' is set in args and
+    // STAR_ALIGN.out.bam_transcript is populated
+    //
+
+    BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME ( STAR_ALIGN.out.bam_transcript, ch_transcripts_fasta )
+    ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME.out.versions)
+
+    emit:
+
+    orig_bam            = STAR_ALIGN.out.bam                                 // channel: [ val(meta), path(bam)            ]
+    log_final           = STAR_ALIGN.out.log_final                           // channel: [ val(meta), path(log_final)      ]
+    log_out             = STAR_ALIGN.out.log_out                             // channel: [ val(meta), path(log_out)        ]
+    log_progress        = STAR_ALIGN.out.log_progress                        // channel: [ val(meta), path(log_progress)   ]
+    bam_sorted          = STAR_ALIGN.out.bam_sorted                          // channel: [ val(meta), path(bam)            ]
+    fastq               = STAR_ALIGN.out.fastq                               // channel: [ val(meta), path(fastq)          ]
+    tab                 = STAR_ALIGN.out.tab                                 // channel: [ val(meta), path(tab)            ]
+    orig_bam_transcript = STAR_ALIGN.out.bam_transcript                      // channel: [ val(meta), path(bam)            ]
+    bam_sorted_aligned  = STAR_ALIGN.out.bam_sorted_aligned                  // channel: [ val(meta), path(bam)            ]
+
+    bam                 = BAM_SORT_STATS_SAMTOOLS_GENOME.out.bam             // channel: [ val(meta), path(bam) ]
+    bai                 = BAM_SORT_STATS_SAMTOOLS_GENOME.out.bai             // channel: [ val(meta), path(bai) ]
+    stats               = BAM_SORT_STATS_SAMTOOLS_GENOME.out.stats           // channel: [ val(meta), path(stats) ]
+    flagstat            = BAM_SORT_STATS_SAMTOOLS_GENOME.out.flagstat        // channel: [ val(meta), path(flagstat) ]
+    idxstats            = BAM_SORT_STATS_SAMTOOLS_GENOME.out.idxstats        // channel: [ val(meta), path(idxstats) ]
+
+    bam_transcript      = BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME.out.bam      // channel: [ val(meta), path(bam) ]
+    bai_transcript      = BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME.out.bai      // channel: [ val(meta), path(bai) ]
+    stats_transcript    = BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME.out.stats    // channel: [ val(meta), path(stats) ]
+    flagstat_transcript = BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME.out.flagstat // channel: [ val(meta), path(flagstat) ]
+    idxstats_transcript = BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME.out.idxstats // channel: [ val(meta), path(idxstats) ]
+
+    versions            = ch_versions                        // channel: [ path(versions.yml) ]
+}
diff --git a/subworkflows/nf-core/fastq_align_star/meta.yml b/subworkflows/nf-core/fastq_align_star/meta.yml
new file mode 100644
index 000000000..796d3e4b6
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/meta.yml
@@ -0,0 +1,133 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "fastq_align_star"
+description: Align reads to a reference genome using bowtie2 then sort with samtools
+keywords:
+  - align
+  - fasta
+  - genome
+  - reference
+components:
+  - star/align
+  - samtools/sort
+  - samtools/index
+  - samtools/stats
+  - samtools/idxstats
+  - samtools/flagstat
+  - bam_sort_stats_samtools
+input:
+  - ch_reads:
+      description: |
+        List of input FastQ files of size 1 and 2 for single-end and paired-end data,
+        respectively.
+        Structure: [ val(meta), [ path(reads) ] ]
+  - ch_index:
+      type: directory
+      description: STAR genome index
+      pattern: "star"
+  - ch_gtf:
+      type: file
+      description: |
+        GTF file used to set the splice junctions with the --sjdbGTFfile flag
+      pattern: "*.gtf"
+  - val_star_ignore_sjdbgtf:
+      type: boolean
+      description: |
+        If true the --sjdbGTFfile flag is set
+      pattern: "true|false"
+  - val_seq_platform:
+      type: string
+      description: |
+        Sequencing platform to be added to the bam header using the --outSAMattrRGline flag
+  - val_seq_center:
+      type: string
+      description: |
+        Sequencing center to be added to the bam header using the --outSAMattrRGline flag
+  - ch_fasta:
+      type: file
+      description: Reference genome fasta file
+      pattern: "*.{fasta,fa,fna}"
+  - ch_transcripts_fasta:
+      type: file
+      description: Optional reference genome fasta file
+      pattern: "*.{fasta,fa,fna}"
+output:
+  - orig_bam:
+      description: |
+        Output BAM file containing read alignments
+        Structure: [ val(meta), path(bam) ]
+  - log_final:
+      description: |
+        STAR final log file
+        Structure: [ val(meta), path(log_final) ]
+  - log_out:
+      description: |
+        STAR log out file
+        Structure: [ val(meta), path(log_out) ]
+  - log_progress:
+      description: |
+        STAR log progress file
+        Structure: [ val(meta), path(log_progress) ]
+  - bam_sorted:
+      description: |
+        Sorted BAM file of read alignments (optional)
+        Structure: [ val(meta), path(bam) ]
+  - orig_bam_transcript:
+      description: |
+        Output BAM file of transcriptome alignment (optional)
+        Structure: [ val(meta), path(bam) ]
+  - fastq:
+      description: |
+        Unmapped FastQ files (optional)
+        Structure: [ val(meta), path(fastq) ]
+  - tab:
+      description: |
+        STAR output tab file(s) (optional)
+        Structure: [ val(meta), path(tab) ]
+  - bam:
+      description: |
+        BAM file ordered by samtools
+        Structure: [ val(meta), path(bam) ]
+  - bai:
+      description: |
+        BAI index of the ordered BAM file
+        Structure: [ val(meta), path(bai) ]
+  - stats:
+      description: |
+        File containing samtools stats output
+        Structure: [ val(meta), path(stats) ]
+  - flagstat:
+      description: |
+        File containing samtools flagstat output
+        Structure: [ val(meta), path(flagstat) ]
+  - idxstats:
+      description: |
+        File containing samtools idxstats output
+        Structure: [ val(meta), path(idxstats) ]
+  - bam_transcript:
+      description: |
+        Transcriptome-level BAM file ordered by samtools  (optional)
+        Structure: [ val(meta), path(bam) ]
+  - bai_transcript:
+      description: |
+        Transcriptome-level BAI index of the ordered BAM file (optional)
+        Structure: [ val(meta), path(bai) ]
+  - stats_transcript:
+      description: |
+        Transcriptome-level file containing samtools stats output (optional)
+        Structure: [ val(meta), path(stats) ]
+  - flagstat_transcript:
+      description: |
+        Transcriptome-level file containing samtools flagstat output (optional)
+        Structure: [ val(meta), path(flagstat) ]
+  - idxstats_transcript:
+      description: |
+        Transcriptome-level file containing samtools idxstats output (optional)
+        Structure: [ val(meta), path(idxstats) ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+authors:
+  - "@JoseEspinosa"
+maintainers:
+  - "@JoseEspinosa"
diff --git a/subworkflows/nf-core/fastq_align_star/nextflow.config b/subworkflows/nf-core/fastq_align_star/nextflow.config
new file mode 100644
index 000000000..926eae710
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/nextflow.config
@@ -0,0 +1,10 @@
+// IMPORTANT: Add this configuration to your modules.config
+
+process {
+    withName: ".*:FASTQ_ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS_GENOME:.*" {
+        ext.prefix = {"${meta.id}_genome"}
+    }
+    withName: ".*:FASTQ_ALIGN_STAR:BAM_SORT_STATS_SAMTOOLS_TRANSCRIPTOME:.*" {
+        ext.prefix = {"${meta.id}_transcriptome"}
+    }
+}
diff --git a/subworkflows/nf-core/fastq_align_star/tests/main.nf.test b/subworkflows/nf-core/fastq_align_star/tests/main.nf.test
new file mode 100644
index 000000000..c1ad73a93
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/tests/main.nf.test
@@ -0,0 +1,250 @@
+nextflow_workflow {
+
+    name "Test Subworkflow FASTQ_ALIGN_STAR"
+    script "../main.nf"
+    workflow "FASTQ_ALIGN_STAR"
+
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "subworkflows/fastq_align_star"
+    tag "star/align"
+    tag "star/genomegenerate"
+    tag "rsem/preparereference"
+    tag "subworkflows/bam_sort_stats_samtools"
+
+    setup {
+        run("STAR_GENOMEGENERATE") {
+            script "../../../../modules/nf-core/star/genomegenerate/main.nf"
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+            }
+        }
+    }
+
+    test("homo_sapiens - fastq - single_end") {
+        config "./nextflow.config"
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:true ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true) ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = true       // star_ignore_sjdbgtf
+                input[4] = 'illumina' // seq_platform
+                input[5] = false      // seq_center
+                input[6] = Channel.of([
+                    [ id:'test_fasta' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[7] = Channel.of([[:], []])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    bam(workflow.out.bam[0][1]).getReadsMD5(),
+                    bam(workflow.out.orig_bam[0][1]).getReadsMD5(),
+                    workflow.out.fastq,
+                    workflow.out.tab,
+                    workflow.out.stats,
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.versions
+                ).match() },
+                { assert path(workflow.out.bai.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_out.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_final.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_progress.get(0).get(1)).exists() }
+            )
+        }
+    }
+
+    test("homo_sapiens - fastq - paired_end") {
+        config "./nextflow.config"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = true       // star_ignore_sjdbgtf
+                input[4] = 'illumina' // seq_platform
+                input[5] = false      // seq_center
+                input[6] = Channel.of([
+                    [ id:'test_fasta' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[7] = Channel.of([[:], []])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    bam(workflow.out.bam[0][1]).getReadsMD5(),
+                    bam(workflow.out.orig_bam[0][1]).getReadsMD5(),
+                    workflow.out.fastq,
+                    workflow.out.tab,
+                    workflow.out.stats,
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.versions
+                ).match() },
+                { assert path(workflow.out.bai.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_out.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_final.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_progress.get(0).get(1)).exists() }
+            )
+        }
+    }
+
+    test("homo_sapiens - fastq - paired_end - with_transcriptome") {
+        config "./with_transcripts.config"
+
+        setup {
+            run("RSEM_PREPAREREFERENCE") {
+                script "../../../../modules/nf-core/rsem/preparereference/main.nf"
+                process {
+                    """
+                    input[0] = channel.of(file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkifexists: true))
+                    input[1] = channel.of(file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkifexists: true))
+                    """
+                }
+            }
+        }
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = true       // star_ignore_sjdbgtf
+                input[4] = 'illumina' // seq_platform
+                input[5] = false      // seq_center
+                input[6] = Channel.of([
+                    [ id:'test_fasta' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[7] = RSEM_PREPAREREFERENCE.out.transcript_fasta.map{[[:], it]}
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    bam(workflow.out.bam[0][1]).getReadsMD5(),
+                    bam(workflow.out.orig_bam[0][1]).getReadsMD5(),
+                    bam(workflow.out.bam_transcript[0][1]).getReadsMD5(),
+                    bam(workflow.out.orig_bam_transcript[0][1]).getReadsMD5(),
+                    workflow.out.fastq,
+                    workflow.out.tab,
+                    workflow.out.stats,
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats_transcript,
+                    workflow.out.flagstat_transcript,
+                    workflow.out.idxstats_transcript,
+                    workflow.out.versions
+                ).match() },
+                { assert path(workflow.out.bai.get(0).get(1)).exists() },
+                { assert path(workflow.out.bai_transcript.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_out.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_final.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_progress.get(0).get(1)).exists() }
+            )
+        }
+    }
+
+    test("homo_sapiens - fastq - paired_end - transcripts - no_transcriptome") {
+        config "./with_transcripts.config"
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                    ]
+                ])
+                input[1] = STAR_GENOMEGENERATE.out.index
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = true       // star_ignore_sjdbgtf
+                input[4] = 'illumina' // seq_platform
+                input[5] = false      // seq_center
+                input[6] = Channel.of([
+                    [ id:'test_fasta' ], // meta map
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[7] = Channel.of([[:], []])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success},
+                { assert snapshot(
+                    bam(workflow.out.bam[0][1]).getReadsMD5(),
+                    bam(workflow.out.orig_bam[0][1]).getReadsMD5(),
+                    workflow.out.fastq,
+                    workflow.out.tab,
+                    workflow.out.stats,
+                    workflow.out.flagstat,
+                    workflow.out.idxstats,
+                    workflow.out.stats_transcript,
+                    workflow.out.versions
+                ).match() },
+                { assert path(workflow.out.bai.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_out.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_final.get(0).get(1)).exists() },
+                { assert path(workflow.out.log_progress.get(0).get(1)).exists() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/fastq_align_star/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_align_star/tests/main.nf.test.snap
new file mode 100644
index 000000000..c21ec0cc9
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/tests/main.nf.test.snap
@@ -0,0 +1,282 @@
+{
+    "homo_sapiens - fastq - paired_end - transcripts - no_transcriptome": {
+        "content": [
+            "1e02b8e6b4a02ab58bfe4ee795aba815",
+            "47a5da42b696886e2ed36af199a9042e",
+            [
+                
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.SJ.out.tab:md5,844af19ab0fc8cd9a3f75228445aca0d"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.stats:md5,d7509941ffbfb221115faa2481115f23"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.flagstat:md5,db0e25cd0b37d3030e807846c022199e"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.idxstats:md5,107ca94dd426cc44db316f0d402307c5"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.stats:md5,10c63f09ff2b849443bafad71a824b0e"
+                ]
+            ],
+            [
+                "versions.yml:md5,167faaf660e4936645b0ad5a9d3bf620",
+                "versions.yml:md5,24b681997fbdd563910240af500d965e",
+                "versions.yml:md5,2bdea75e8dad77676c54b806ceecb68b",
+                "versions.yml:md5,2e0fd05eea17eb1f5cdf079c12a30b88",
+                "versions.yml:md5,421308f325b1038feafdb6862cebc28a",
+                "versions.yml:md5,63c683e2834a8968f4d626dbe9b7a07f",
+                "versions.yml:md5,6a96fec10a72e1da236e6e1d7519590e",
+                "versions.yml:md5,9dd7217e54100569bf9f87f7a1cca54a",
+                "versions.yml:md5,a305cb08e7cb3194be54d0bb8a257722",
+                "versions.yml:md5,ade8e9901ff456f43bc0f93f5f7af793",
+                "versions.yml:md5,ec328c7ea462702388fa15204aeb6689"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-11T17:09:46.703861921"
+    },
+    "homo_sapiens - fastq - paired_end - with_transcriptome": {
+        "content": [
+            "1e02b8e6b4a02ab58bfe4ee795aba815",
+            "47a5da42b696886e2ed36af199a9042e",
+            "9b8ec790f8138071a0731b5225c84f00",
+            "9b8ec790f8138071a0731b5225c84f00",
+            [
+                
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.SJ.out.tab:md5,844af19ab0fc8cd9a3f75228445aca0d"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.stats:md5,d7509941ffbfb221115faa2481115f23"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.flagstat:md5,db0e25cd0b37d3030e807846c022199e"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.idxstats:md5,107ca94dd426cc44db316f0d402307c5"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.stats:md5,10ca84181b17dafd7248296114f05333"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.flagstat:md5,a204dee59ef8b7cd0f7d952a80119b77"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.idxstats:md5,4030919b4a05393dcd7e699d72725803"
+                ]
+            ],
+            [
+                "versions.yml:md5,167faaf660e4936645b0ad5a9d3bf620",
+                "versions.yml:md5,24b681997fbdd563910240af500d965e",
+                "versions.yml:md5,2bdea75e8dad77676c54b806ceecb68b",
+                "versions.yml:md5,2e0fd05eea17eb1f5cdf079c12a30b88",
+                "versions.yml:md5,421308f325b1038feafdb6862cebc28a",
+                "versions.yml:md5,63c683e2834a8968f4d626dbe9b7a07f",
+                "versions.yml:md5,6a96fec10a72e1da236e6e1d7519590e",
+                "versions.yml:md5,9dd7217e54100569bf9f87f7a1cca54a",
+                "versions.yml:md5,a305cb08e7cb3194be54d0bb8a257722",
+                "versions.yml:md5,ade8e9901ff456f43bc0f93f5f7af793",
+                "versions.yml:md5,ec328c7ea462702388fa15204aeb6689"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-11T17:08:59.431516768"
+    },
+    "homo_sapiens - fastq - single_end": {
+        "content": [
+            "8b3699d1b3344c90f82363edadae6427",
+            "c491ec34a25f55c7583e325e0a191b5d",
+            [
+                
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.SJ.out.tab:md5,75a516ab950fb958f40b29996474949c"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.sorted.bam.stats:md5,30abf517cda76c6a7468541781c4dd7a"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.sorted.bam.flagstat:md5,075e7a684a7ceb5fd1dae154f823128a"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": true
+                    },
+                    "test.sorted.bam.idxstats:md5,0d5a12e3e3ffdb15f1c6c50f02f22575"
+                ]
+            ],
+            [
+                "versions.yml:md5,167faaf660e4936645b0ad5a9d3bf620",
+                "versions.yml:md5,24b681997fbdd563910240af500d965e",
+                "versions.yml:md5,2e0fd05eea17eb1f5cdf079c12a30b88",
+                "versions.yml:md5,421308f325b1038feafdb6862cebc28a",
+                "versions.yml:md5,6a96fec10a72e1da236e6e1d7519590e",
+                "versions.yml:md5,9dd7217e54100569bf9f87f7a1cca54a"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-11T17:07:23.833781959"
+    },
+    "homo_sapiens - fastq - paired_end": {
+        "content": [
+            "1e02b8e6b4a02ab58bfe4ee795aba815",
+            "47a5da42b696886e2ed36af199a9042e",
+            [
+                
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.SJ.out.tab:md5,844af19ab0fc8cd9a3f75228445aca0d"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.stats:md5,d7509941ffbfb221115faa2481115f23"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.flagstat:md5,db0e25cd0b37d3030e807846c022199e"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.idxstats:md5,107ca94dd426cc44db316f0d402307c5"
+                ]
+            ],
+            [
+                "versions.yml:md5,167faaf660e4936645b0ad5a9d3bf620",
+                "versions.yml:md5,24b681997fbdd563910240af500d965e",
+                "versions.yml:md5,2e0fd05eea17eb1f5cdf079c12a30b88",
+                "versions.yml:md5,421308f325b1038feafdb6862cebc28a",
+                "versions.yml:md5,6a96fec10a72e1da236e6e1d7519590e",
+                "versions.yml:md5,9dd7217e54100569bf9f87f7a1cca54a"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "24.10.2"
+        },
+        "timestamp": "2024-12-11T17:08:08.904146433"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/fastq_align_star/tests/nextflow.config b/subworkflows/nf-core/fastq_align_star/tests/nextflow.config
new file mode 100644
index 000000000..6af49d586
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/tests/nextflow.config
@@ -0,0 +1,21 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+
+    withName: STAR_GENOMEGENERATE {
+        ext.args = '--genomeSAindexNbases 9'
+    }
+
+    withName: STAR_ALIGN {
+        ext.args = '--readFilesCommand zcat'
+    }
+
+    withName: '.*:BAM_SORT_STATS_SAMTOOLS_.*:SAMTOOLS_.*' {
+        ext.prefix = { "${meta.id}.sorted" }
+    }
+
+    withName: '.*:BAM_SORT_STATS_SAMTOOLS_.*:BAM_STATS_SAMTOOLS:.*' {
+        ext.prefix = { "${meta.id}.sorted.bam" }
+    }
+
+}
diff --git a/subworkflows/nf-core/fastq_align_star/tests/with_transcripts.config b/subworkflows/nf-core/fastq_align_star/tests/with_transcripts.config
new file mode 100644
index 000000000..62f152501
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/tests/with_transcripts.config
@@ -0,0 +1,21 @@
+process {
+
+    publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
+
+    withName: STAR_GENOMEGENERATE {
+        ext.args = '--genomeSAindexNbases 9'
+    }
+
+    withName: STAR_ALIGN {
+        ext.args = '--readFilesCommand zcat --quantMode TranscriptomeSAM'
+    }
+
+    withName: '.*:BAM_SORT_STATS_SAMTOOLS_.*:SAMTOOLS_.*' {
+        ext.prefix = { "${meta.id}.sorted" }
+    }
+
+    withName: '.*:BAM_SORT_STATS_SAMTOOLS_.*:BAM_STATS_SAMTOOLS:.*' {
+        ext.prefix = { "${meta.id}.sorted.bam" }
+    }
+
+}
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index 06a5dd26c..dbb5e726e 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -18,8 +18,9 @@ include { MULTIQC                       }   from '../modules/nf-core/multiqc/mai
 include { STAR_ALIGN                    }   from '../modules/nf-core/star/align/main'
 include { SALMON_QUANT                  }   from '../modules/nf-core/salmon/quant/main'
 include { SAMTOOLS_CONVERT              }   from '../modules/nf-core/samtools/convert/main'
+include { SAMTOOLS_INDEX                }   from '../modules/nf-core/samtools/index/main'
 include { paramsSummaryMap              }   from 'plugin/nf-schema'
-include { FASTQ_ALIGN_STAR              }   from '../subworkflows/local/fastq_align_star'
+include { FASTQ_ALIGN_STAR              }   from '../subworkflows/nf-core/fastq_align_star'
 include { paramsSummaryMultiqc          }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML        }   from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { methodsDescriptionText        }   from '../subworkflows/local/utils_nfcore_rnafusion_pipeline'
@@ -181,13 +182,18 @@ workflow RNAFUSION {
                 ch_fastqs_to_align,
                 BUILD_REFERENCES.out.starindex_ref,
                 BUILD_REFERENCES.out.gtf,
-                BUILD_REFERENCES.out.fasta,
-                BUILD_REFERENCES.out.fai,
                 params.star_ignore_sjdbgtf,
-                params.cram
+                ch_fastqs_to_align.map { meta, _fastqs -> meta.seq_platform },
+                ch_fastqs_to_align.map { meta, _fastqs -> meta.seq_center },
+                BUILD_REFERENCES.out.fasta,
+                [[:], []]
             )
+            FASTQ_ALIGN_STAR.out.bam_sorted_aligned.view()
+            SAMTOOLS_INDEX(FASTQ_ALIGN_STAR.out.bam_sorted_aligned)
+            ch_bam_bai = FASTQ_ALIGN_STAR.out.bam_sorted_aligned
+                .join(SAMTOOLS_INDEX.out.bai, failOnMismatch:true, failOnDuplicate:true)
             ch_versions             = ch_versions.mix(FASTQ_ALIGN_STAR.out.versions)
-            ch_aligned_reads        = ch_aligned_reads.mix(FASTQ_ALIGN_STAR.out.bam_bai)
+            ch_aligned_reads        = ch_aligned_reads.mix(ch_bam_bai)
             ch_star_junctions       = ch_star_junctions.mix(FASTQ_ALIGN_STAR.out.junctions)
             ch_star_splice_junctions = ch_star_splice_junctions.mix(FASTQ_ALIGN_STAR.out.spl_junc_tabs)
             ch_multiqc_files        = ch_multiqc_files.mix(FASTQ_ALIGN_STAR.out.log_final.collect{it[1]}.ifEmpty([]))

From baf957e35c074c18f720aa2a8fab40dd7acf476c Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Tue, 26 Aug 2025 23:35:48 +0200
Subject: [PATCH 801/887] Add emits to fastq_align_star subworkflow

---
 .../nf-core/bam_sort_stats_samtools/main.nf   |  2 +-
 subworkflows/nf-core/fastq_align_star/main.nf |  3 ++
 tests/test_stub_bam.nf.test.snap              | 28 +++++++++----------
 3 files changed, 18 insertions(+), 15 deletions(-)

diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
index 460726368..b716375b0 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
@@ -15,7 +15,7 @@ workflow BAM_SORT_STATS_SAMTOOLS {
 
     ch_versions = Channel.empty()
 
-    SAMTOOLS_SORT ( ch_bam, ch_fasta, '' )
+    SAMTOOLS_SORT ( ch_bam, ch_fasta )
     ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first())
 
     SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam )
diff --git a/subworkflows/nf-core/fastq_align_star/main.nf b/subworkflows/nf-core/fastq_align_star/main.nf
index 4b3602982..c4b382ebb 100644
--- a/subworkflows/nf-core/fastq_align_star/main.nf
+++ b/subworkflows/nf-core/fastq_align_star/main.nf
@@ -52,6 +52,9 @@ workflow FASTQ_ALIGN_STAR {
     tab                 = STAR_ALIGN.out.tab                                 // channel: [ val(meta), path(tab)            ]
     orig_bam_transcript = STAR_ALIGN.out.bam_transcript                      // channel: [ val(meta), path(bam)            ]
     bam_sorted_aligned  = STAR_ALIGN.out.bam_sorted_aligned                  // channel: [ val(meta), path(bam)            ]
+    junctions           = STAR_ALIGN.out.junction                            // channel: [ val(meta), path(junction)       ]
+    spl_junc_tabs       = STAR_ALIGN.out.spl_junc_tab                        // channel: [ val(meta), path(spl_junc_tab)   ]
+    gene_count          = STAR_ALIGN.out.read_per_gene_tab                   // channel: [ val(meta), path(read_tab)       ]
 
     bam                 = BAM_SORT_STATS_SAMTOOLS_GENOME.out.bam             // channel: [ val(meta), path(bam) ]
     bai                 = BAM_SORT_STATS_SAMTOOLS_GENOME.out.bai             // channel: [ val(meta), path(bai) ]
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 762751f28..e4a6a04be 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -189,9 +189,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T15:54:01.219397324"
+        "timestamp": "2025-08-26T23:14:20.283916876"
     },
     "stub test stringtie": {
         "content": [
@@ -311,9 +311,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-08T11:34:57.146117646"
+        "timestamp": "2025-08-26T23:15:30.482022054"
     },
     "stub test arriba": {
         "content": [
@@ -423,9 +423,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-08T11:31:27.759259528"
+        "timestamp": "2025-08-26T22:48:22.193453934"
     },
     "stub test fusioninspector": {
         "content": [
@@ -673,9 +673,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T16:00:15.182860428"
+        "timestamp": "2025-08-26T23:30:41.022404222"
     },
     "stub test salmon": {
         "content": [
@@ -1000,9 +1000,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T15:47:14.034109802"
+        "timestamp": "2025-08-26T23:06:10.100949608"
     },
     "stub test fusionreport": {
         "content": [
@@ -1227,9 +1227,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T15:56:39.559031879"
+        "timestamp": "2025-08-26T23:22:51.682095887"
     },
     "stub test fastp": {
         "content": [
@@ -1762,8 +1762,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T15:39:59.605183405"
+        "timestamp": "2025-08-26T23:00:13.773945342"
     }
 }
\ No newline at end of file

From 859edab5061af2aab5a9572cb447191a61714af1 Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Wed, 27 Aug 2025 15:55:03 +0200
Subject: [PATCH 802/887] Update stub test

---
 tests/test_stub.nf.test.snap | 175 ++++++++++++++++++++++++++++++-----
 1 file changed, 154 insertions(+), 21 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 759d060d9..61f9424c2 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -1,7 +1,7 @@
 {
     "stub test starfusion": {
         "content": [
-            17,
+            27,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -37,9 +37,21 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
@@ -189,7 +201,14 @@
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.Aligned.sortedByCoord.bam.csi",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
                 "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "star/test.Aligned.unsort.out.bam",
@@ -216,13 +235,13 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T16:11:25.285702343"
+        "timestamp": "2025-08-27T15:23:50.746792719"
     },
     "stub test stringtie": {
         "content": [
-            17,
+            27,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -258,9 +277,21 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -332,7 +363,14 @@
                 "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
                 "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
                 "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.Aligned.sortedByCoord.bam.csi",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
                 "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "star/test.Aligned.unsort.out.bam",
@@ -362,13 +400,13 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-08T11:20:46.857913005"
+        "timestamp": "2025-08-27T15:25:28.858027686"
     },
     "stub test arriba": {
         "content": [
-            16,
+            26,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -407,9 +445,21 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
                 "STAR_ALIGN": {
                     "star": "2.7.11b",
                     "samtools": 1.21,
@@ -478,7 +528,14 @@
                 "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
                 "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
                 "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.Aligned.sortedByCoord.bam.csi",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
                 "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "star/test.Aligned.unsort.out.bam",
@@ -501,13 +558,13 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-08T11:16:32.896368369"
+        "timestamp": "2025-08-27T15:16:09.411578571"
     },
     "stub test fusioninspector": {
         "content": [
-            24,
+            34,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -564,9 +621,21 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
@@ -750,7 +819,14 @@
                 "references/GRCh38/hgnc",
                 "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
                 "references/GRCh38/hgnc/hgnc_complete_set.txt",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.Aligned.sortedByCoord.bam.csi",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
                 "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "star/test.Aligned.unsort.out.bam",
@@ -775,9 +851,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T16:16:11.496999226"
+        "timestamp": "2025-08-27T15:34:06.043690619"
     },
     "stub test salmon": {
         "content": [
@@ -876,7 +952,7 @@
     },
     "stub test ctatsplicing": {
         "content": [
-            18,
+            28,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -918,9 +994,21 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
@@ -1084,7 +1172,14 @@
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.Aligned.sortedByCoord.bam.csi",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
                 "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "star/test.Aligned.unsort.out.bam",
@@ -1107,13 +1202,13 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T16:08:54.904059765"
+        "timestamp": "2025-08-27T15:19:10.863659919"
     },
     "stub test fusionreport": {
         "content": [
-            21,
+            31,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1161,9 +1256,21 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
@@ -1334,7 +1441,14 @@
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.Aligned.sortedByCoord.bam.csi",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
                 "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "star/test.Aligned.unsort.out.bam",
@@ -1361,9 +1475,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T16:13:31.815555556"
+        "timestamp": "2025-08-27T15:28:20.168052482"
     },
     "stub test fastp": {
         "content": [
@@ -1600,7 +1714,7 @@
     },
     "stub test all": {
         "content": [
-            35,
+            45,
             {
                 "AGAT_CONVERTGFF2BED": {
                     "agat": "v1.5.1"
@@ -1681,9 +1795,21 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
                 "SAMTOOLS_INDEX": {
                     "samtools": 1.21
                 },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
                 "STARFUSION_BUILD": {
                     "gunzip": 1.12,
                     "hmmer": 3.4,
@@ -1927,7 +2053,14 @@
                 "salmon/test",
                 "salmon/test_lib_format_counts.json",
                 "salmon/test_meta_info.json",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.Aligned.sortedByCoord.bam.csi",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
                 "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
                 "star/test.Aligned.sortedByCoord.out.bam",
                 "star/test.Aligned.sortedByCoord.out.bam.bai",
                 "star/test.Aligned.unsort.out.bam",
@@ -1963,8 +2096,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-18T16:02:59.744224309"
+        "timestamp": "2025-08-27T15:15:02.845874526"
     }
 }
\ No newline at end of file

From 5071899d9fad6f3c5909967b860b58be8c1f9f98 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Wed, 27 Aug 2025 14:12:53 +0000
Subject: [PATCH 803/887] Patch subworkflows to avoid lint failures

---
 .../bam_sort_stats_samtools.diff              | 18 +++++++++++++++
 .../fastq_align_star/fastq_align_star.diff    | 23 +++++++++++++++++++
 2 files changed, 41 insertions(+)
 create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff
 create mode 100644 subworkflows/nf-core/fastq_align_star/fastq_align_star.diff

diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff b/subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff
new file mode 100644
index 000000000..74c35330d
--- /dev/null
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff
@@ -0,0 +1,18 @@
+Changes in component 'nf-core/bam_sort_stats_samtools'
+Changes in 'bam_sort_stats_samtools/main.nf':
+--- subworkflows/nf-core/bam_sort_stats_samtools/main.nf
++++ subworkflows/nf-core/bam_sort_stats_samtools/main.nf
+@@ -15,7 +15,7 @@
+ 
+     ch_versions = Channel.empty()
+ 
+-    SAMTOOLS_SORT ( ch_bam, ch_fasta, '' )
++    SAMTOOLS_SORT ( ch_bam, ch_fasta )
+     ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first())
+ 
+     SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam )
+
+'subworkflows/nf-core/bam_sort_stats_samtools/meta.yml' is unchanged
+'subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test' is unchanged
+'subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap' is unchanged
+************************************************************
diff --git a/subworkflows/nf-core/fastq_align_star/fastq_align_star.diff b/subworkflows/nf-core/fastq_align_star/fastq_align_star.diff
new file mode 100644
index 000000000..1f4a289e5
--- /dev/null
+++ b/subworkflows/nf-core/fastq_align_star/fastq_align_star.diff
@@ -0,0 +1,23 @@
+Changes in component 'nf-core/fastq_align_star'
+Changes in 'fastq_align_star/main.nf':
+--- subworkflows/nf-core/fastq_align_star/main.nf
++++ subworkflows/nf-core/fastq_align_star/main.nf
+@@ -51,6 +51,10 @@
+     fastq               = STAR_ALIGN.out.fastq                               // channel: [ val(meta), path(fastq)          ]
+     tab                 = STAR_ALIGN.out.tab                                 // channel: [ val(meta), path(tab)            ]
+     orig_bam_transcript = STAR_ALIGN.out.bam_transcript                      // channel: [ val(meta), path(bam)            ]
++    bam_sorted_aligned  = STAR_ALIGN.out.bam_sorted_aligned                  // channel: [ val(meta), path(bam)            ]
++    junctions           = STAR_ALIGN.out.junction                            // channel: [ val(meta), path(junction)       ]
++    spl_junc_tabs       = STAR_ALIGN.out.spl_junc_tab                        // channel: [ val(meta), path(spl_junc_tab)   ]
++    gene_count          = STAR_ALIGN.out.read_per_gene_tab                   // channel: [ val(meta), path(read_tab)       ]
+ 
+     bam                 = BAM_SORT_STATS_SAMTOOLS_GENOME.out.bam             // channel: [ val(meta), path(bam) ]
+     bai                 = BAM_SORT_STATS_SAMTOOLS_GENOME.out.bai             // channel: [ val(meta), path(bai) ]
+
+'subworkflows/nf-core/fastq_align_star/meta.yml' is unchanged
+'subworkflows/nf-core/fastq_align_star/nextflow.config' is unchanged
+'subworkflows/nf-core/fastq_align_star/tests/main.nf.test' is unchanged
+'subworkflows/nf-core/fastq_align_star/tests/main.nf.test.snap' is unchanged
+'subworkflows/nf-core/fastq_align_star/tests/nextflow.config' is unchanged
+'subworkflows/nf-core/fastq_align_star/tests/with_transcripts.config' is unchanged
+************************************************************

From 8282dc06ef04448075529f67183a6595fad4f0a1 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <terradasdelfi@gmail.com>
Date: Wed, 27 Aug 2025 14:21:39 +0000
Subject: [PATCH 804/887] Update santools/sort and keep original subworkflow
 bam_sort_stats_samtools

---
 modules.json                                  |   2 +-
 modules/nf-core/samtools/sort/main.nf         |  59 ++--
 modules/nf-core/samtools/sort/meta.yml        |  67 +++--
 .../nf-core/samtools/sort/tests/main.nf.test  | 144 +++++++++-
 .../samtools/sort/tests/main.nf.test.snap     | 272 +++++++++++++-----
 .../samtools/sort/tests/nextflow.config       |   1 -
 .../bam_sort_stats_samtools.diff              |  18 --
 .../nf-core/bam_sort_stats_samtools/main.nf   |   2 +-
 8 files changed, 437 insertions(+), 128 deletions(-)
 delete mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff

diff --git a/modules.json b/modules.json
index 463d52bd1..904735b15 100644
--- a/modules.json
+++ b/modules.json
@@ -159,7 +159,7 @@
                     },
                     "samtools/sort": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "e89ff68a46b848c4d8aabb8569767516d46db319",
                         "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/stats": {
diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf
index caf3c61a8..6c436c043 100644
--- a/modules/nf-core/samtools/sort/main.nf
+++ b/modules/nf-core/samtools/sort/main.nf
@@ -10,24 +10,35 @@ process SAMTOOLS_SORT {
     input:
     tuple val(meta) , path(bam)
     tuple val(meta2), path(fasta)
+    val index_format
 
     output:
-    tuple val(meta), path("*.bam"),  emit: bam,  optional: true
-    tuple val(meta), path("*.cram"), emit: cram, optional: true
-    tuple val(meta), path("*.crai"), emit: crai, optional: true
-    tuple val(meta), path("*.csi"),  emit: csi,  optional: true
-    path  "versions.yml",            emit: versions
+    tuple val(meta), path("${prefix}.bam"),                 emit: bam,  optional: true
+    tuple val(meta), path("${prefix}.cram"),                emit: cram, optional: true
+    tuple val(meta), path("${prefix}.sam"),                 emit: sam,  optional: true
+    tuple val(meta), path("${prefix}.${extension}.crai"),   emit: crai, optional: true
+    tuple val(meta), path("${prefix}.${extension}.csi"),    emit: csi,  optional: true
+    tuple val(meta), path("${prefix}.${extension}.bai"),    emit: bai,  optional: true
+    path  "versions.yml",                                   emit: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-fmt sam") ? "sam" :
-                    args.contains("--output-fmt cram") ? "cram" :
-                    "bam"
-    def reference = fasta ? "--reference ${fasta}" : ""
+    prefix = task.ext.prefix ?: "${meta.id}"
+    extension = args.contains("--output-fmt sam") ? "sam" :
+                args.contains("--output-fmt cram") ? "cram" :
+                "bam"
+    reference = fasta ? "--reference ${fasta}" : ""
+    output_file = index_format ? "${prefix}.${extension}##idx##${prefix}.${extension}.${index_format} --write-index" : "${prefix}.${extension}"
+    if (index_format) {
+        if (!index_format.matches('bai|csi|crai')) {
+            error "Index format not one of bai, csi, crai."
+        } else if (extension == "sam") {
+            error "Indexing not compatible with SAM output"
+        }
+    }
     if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
 
     """
@@ -39,7 +50,7 @@ process SAMTOOLS_SORT {
         -T ${prefix} \\
         --threads $task.cpus \\
         ${reference} \\
-        -o ${prefix}.${extension} \\
+        -o ${output_file} \\
         -
 
     cat <<-END_VERSIONS > versions.yml
@@ -50,19 +61,23 @@ process SAMTOOLS_SORT {
 
     stub:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-fmt sam") ? "sam" :
-                    args.contains("--output-fmt cram") ? "cram" :
-                    "bam"
+    prefix = task.ext.prefix ?: "${meta.id}"
+    extension = args.contains("--output-fmt sam") ? "sam" :
+                args.contains("--output-fmt cram") ? "cram" :
+                "bam"
+    def reference = fasta ? "--reference ${fasta}" : ""
+    if (index_format) {
+        if (!index_format.matches('bai|csi|crai')) {
+            error "Index format not one of bai, csi, crai."
+        } else if (extension == "sam") {
+            error "Indexing not compatible with SAM output"
+        }
+    }
+    index = index_format ? "touch ${prefix}.${extension}.${index_format}" : ""
+
     """
     touch ${prefix}.${extension}
-    if [ "${extension}" == "bam" ];
-    then
-        touch ${prefix}.${extension}.csi
-    elif [ "${extension}" == "cram" ];
-    then
-        touch ${prefix}.${extension}.crai
-    fi
+    ${index}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml
index a9dbec5a8..4c4010bbb 100644
--- a/modules/nf-core/samtools/sort/meta.yml
+++ b/modules/nf-core/samtools/sort/meta.yml
@@ -26,6 +26,7 @@ input:
         type: file
         description: BAM/CRAM/SAM file(s)
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -36,52 +37,86 @@ input:
         description: Reference genome FASTA file
         pattern: "*.{fa,fasta,fna}"
         optional: true
+        ontologies: []
+  - index_format:
+      type: string
+      description: Index format to use (optional)
+      pattern: "bai|csi|crai"
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.bam":
+      - "${prefix}.bam":
           type: file
           description: Sorted BAM file
           pattern: "*.{bam}"
-  - cram:
-      - meta:
+          ontologies: []
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.cram":
+      - "${prefix}.cram":
           type: file
           description: Sorted CRAM file
           pattern: "*.{cram}"
-  - crai:
-      - meta:
+          ontologies: []
+  sam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.crai":
+      - "${prefix}.sam":
+          type: file
+          description: Sorted SAM file
+          pattern: "*.{sam}"
+          ontologies: []
+
+  crai:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "${prefix}.${extension}.crai":
           type: file
           description: CRAM index file (optional)
           pattern: "*.crai"
-  - csi:
-      - meta:
+          ontologies: []
+  csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.csi":
+      - "${prefix}.${extension}.csi":
           type: file
           description: BAM index file (optional)
           pattern: "*.csi"
-  - versions:
-      - versions.yml:
+          ontologies: []
+  bai:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "${prefix}.${extension}.bai":
           type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          description: BAM index file (optional)
+          pattern: "*.bai"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@drpatelh"
   - "@ewels"
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test
index b05e6691b..fa2778722 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test
@@ -8,7 +8,7 @@ nextflow_process {
     tag "samtools"
     tag "samtools/sort"
 
-    test("bam") {
+    test("bam_no_index") {
 
         config "./nextflow.config"
 
@@ -23,6 +23,7 @@ nextflow_process {
                     [ id:'fasta' ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
                 ])
+                input[2] = ''
                 """
             }
         }
@@ -32,7 +33,71 @@ nextflow_process {
                 { assert process.success },
                 { assert snapshot(
                         process.out.bam,
-                        process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                        process.out.bai,
+                        process.out.versions
+                ).match()}
+            )
+        }
+    }
+
+    test("bam_bai_index") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = 'bai'
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.bai,
+                        process.out.versions
+                ).match()}
+            )
+        }
+    }
+    
+    test("bam_csi_index") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = 'csi'
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.csi,
                         process.out.versions
                 ).match()}
             )
@@ -57,6 +122,77 @@ nextflow_process {
                     [ id:'fasta' ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
                 ])
+                input[2] = ''
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                        process.out.versions
+                ).match()}
+            )
+        }
+    }
+
+    test("multiple bam bai index") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = 'bai'
+                """
+            }
+        }
+
+        then {
+            assertAll (
+                { assert process.success },
+                { assert snapshot(
+                        process.out.bam,
+                        process.out.bai.collect { it.collect { it instanceof Map ? it : file(it).name } },
+                        process.out.versions
+                ).match()}
+            )
+        }
+    }
+    
+    test("multiple bam csi index") {
+
+        config "./nextflow.config"
+
+        when {
+            process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test', single_end:false ], // meta map
+                    [
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true),
+                        file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)
+                    ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'fasta' ], // meta map
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
+                ])
+                input[2] = 'csi'
                 """
             }
         }
@@ -88,6 +224,7 @@ nextflow_process {
                     [ id:'fasta' ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
                 ])
+                input[2] = ''
                 """
             }
         }
@@ -120,6 +257,7 @@ nextflow_process {
                     [ id:'fasta' ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)
                 ])
+                input[2] = ''
                 """
             }
         }
@@ -150,6 +288,7 @@ nextflow_process {
                     [ id:'fasta' ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
                 ])
+                input[2] = ''
                 """
             }
         }
@@ -178,6 +317,7 @@ nextflow_process {
                     [ id:'fasta' ], // meta map
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)
                 ])
+                input[2] = ''
                 """
             }
         }
diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
index 469891fe3..84e728024 100644
--- a/modules/nf-core/samtools/sort/tests/main.nf.test.snap
+++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap
@@ -24,10 +24,10 @@
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-09-16T08:49:58.207549273"
+        "timestamp": "2025-08-13T16:39:40.803476526"
     },
     "bam - stub": {
         "content": [
@@ -48,16 +48,19 @@
                     
                 ],
                 "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
+                    
                 ],
                 "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
                     "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ],
+                "bai": [
+                    
                 ],
                 "bam": [
                     [
@@ -75,13 +78,10 @@
                     
                 ],
                 "csi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.sorted.bam.csi:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
+                    
+                ],
+                "sam": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,2659b187d681241451539d4c53500b9f"
@@ -89,10 +89,70 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T16:39:46.797296642"
+    },
+    "bam_csi_index": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,d9645c9b6d7f991426d47ad80c275ef7"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.csi:md5,124e6600a0a811227c43fd6864c72902"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T16:39:13.394881233"
+    },
+    "multiple bam bai index": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,0669e48038989db924a9f2957e26317c"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.bai"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-09-16T08:50:08.630951018"
+        "timestamp": "2025-08-13T16:39:28.192036852"
     },
     "cram - stub": {
         "content": [
@@ -110,31 +170,28 @@
                     ]
                 ],
                 "2": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
+                    
                 ],
                 "3": [
                     
                 ],
                 "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
                     "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ],
+                "bai": [
+                    
                 ],
                 "bam": [
                     
                 ],
                 "crai": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.sorted.cram.crai:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
+                    
                 ],
                 "cram": [
                     [
@@ -147,6 +204,9 @@
                 ],
                 "csi": [
                     
+                ],
+                "sam": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,2659b187d681241451539d4c53500b9f"
@@ -154,10 +214,10 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-09-16T08:50:19.061912443"
+        "timestamp": "2025-08-13T16:39:58.847704746"
     },
     "multiple bam": {
         "content": [
@@ -167,27 +227,21 @@
                         "id": "test",
                         "single_end": false
                     },
-                    "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                    "test.sorted.bam:md5,c6ab045cc495485d3819bd44c5d69b92"
                 ]
             ],
             [
-                [
-                    {
-                        "id": "test",
-                        "single_end": false
-                    },
-                    "test.sorted.bam.csi"
-                ]
+                
             ],
             [
                 "versions.yml:md5,2659b187d681241451539d4c53500b9f"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.09.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-10-08T11:59:55.479443"
+        "timestamp": "2025-08-13T14:00:50.550947084"
     },
     "multiple bam - stub": {
         "content": [
@@ -198,7 +252,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                        "test.sorted.bam:md5,c6ab045cc495485d3819bd44c5d69b92"
                     ]
                 ],
                 "1": [
@@ -208,16 +262,19 @@
                     
                 ],
                 "3": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.sorted.bam.csi:md5,d185916eaff9afeb4d0aeab3310371f9"
-                    ]
+                    
                 ],
                 "4": [
+                    
+                ],
+                "5": [
+                    
+                ],
+                "6": [
                     "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+                ],
+                "bai": [
+                    
                 ],
                 "bam": [
                     [
@@ -225,7 +282,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.sorted.bam:md5,8a16ba90c7d294cbb4c33ac0f7127a12"
+                        "test.sorted.bam:md5,c6ab045cc495485d3819bd44c5d69b92"
                     ]
                 ],
                 "crai": [
@@ -235,13 +292,10 @@
                     
                 ],
                 "csi": [
-                    [
-                        {
-                            "id": "test",
-                            "single_end": false
-                        },
-                        "test.sorted.bam.csi:md5,d185916eaff9afeb4d0aeab3310371f9"
-                    ]
+                    
+                ],
+                "sam": [
+                    
                 ],
                 "versions": [
                     "versions.yml:md5,2659b187d681241451539d4c53500b9f"
@@ -249,10 +303,64 @@
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.09.0"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T14:02:05.837266709"
+    },
+    "bam_no_index": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,7fa751088f8d86c2685fb50a8b90f53f"
+                ]
+            ],
+            [
+                
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-10-08T11:36:13.781404"
+        "timestamp": "2025-08-13T14:00:20.988801663"
+    },
+    "multiple bam csi index": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,3fe979e8bd453d33224706d1478df7a1"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.csi"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T16:39:34.591655792"
     },
     "bam": {
         "content": [
@@ -283,5 +391,35 @@
             "nextflow": "24.09.0"
         },
         "timestamp": "2024-10-08T11:59:46.372244"
+    },
+    "bam_bai_index": {
+        "content": [
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam:md5,31e1c0224336156ea9ca81dcfe23356d"
+                ]
+            ],
+            [
+                [
+                    {
+                        "id": "test",
+                        "single_end": false
+                    },
+                    "test.sorted.bam.bai:md5,0575b098cbea8c3af1f58d2a83efa07f"
+                ]
+            ],
+            [
+                "versions.yml:md5,2659b187d681241451539d4c53500b9f"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-13T16:39:06.968372189"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/samtools/sort/tests/nextflow.config b/modules/nf-core/samtools/sort/tests/nextflow.config
index f642771f5..723f62b21 100644
--- a/modules/nf-core/samtools/sort/tests/nextflow.config
+++ b/modules/nf-core/samtools/sort/tests/nextflow.config
@@ -2,7 +2,6 @@ process {
 
     withName: SAMTOOLS_SORT {
         ext.prefix  = { "${meta.id}.sorted" }
-        ext.args    = "--write-index"
     }
 
 }
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff b/subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff
deleted file mode 100644
index 74c35330d..000000000
--- a/subworkflows/nf-core/bam_sort_stats_samtools/bam_sort_stats_samtools.diff
+++ /dev/null
@@ -1,18 +0,0 @@
-Changes in component 'nf-core/bam_sort_stats_samtools'
-Changes in 'bam_sort_stats_samtools/main.nf':
---- subworkflows/nf-core/bam_sort_stats_samtools/main.nf
-+++ subworkflows/nf-core/bam_sort_stats_samtools/main.nf
-@@ -15,7 +15,7 @@
- 
-     ch_versions = Channel.empty()
- 
--    SAMTOOLS_SORT ( ch_bam, ch_fasta, '' )
-+    SAMTOOLS_SORT ( ch_bam, ch_fasta )
-     ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first())
- 
-     SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam )
-
-'subworkflows/nf-core/bam_sort_stats_samtools/meta.yml' is unchanged
-'subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test' is unchanged
-'subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap' is unchanged
-************************************************************
diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
index b716375b0..460726368 100644
--- a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
+++ b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf
@@ -15,7 +15,7 @@ workflow BAM_SORT_STATS_SAMTOOLS {
 
     ch_versions = Channel.empty()
 
-    SAMTOOLS_SORT ( ch_bam, ch_fasta )
+    SAMTOOLS_SORT ( ch_bam, ch_fasta, '' )
     ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first())
 
     SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam )

From 4719efcb6498c1f85e75d4fcf5f424f15519811c Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Wed, 27 Aug 2025 16:31:02 +0200
Subject: [PATCH 805/887] Use smaller dfam and pfam files in tests

---
 tests/nextflow.config | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/tests/nextflow.config b/tests/nextflow.config
index 66ddf0787..eef755fe4 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -24,6 +24,12 @@ process {
 }
 
 params {
+    dfam_hmm                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm"
+    dfam_h3f                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3f"
+    dfam_h3i                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3i"
+    dfam_h3m                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3m"
+    dfam_h3p                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3p"
+    pfam_file                   = "${pipelines_testdata_base_path}/testdata/human/Pfam-A.hmm.gz"
     fusioncatcher_ref           = "${projectDir}/assets"
     arriba_ref_blacklist        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
     arriba_ref_cytobands        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/cytobands_hg38_GRCh38_v2.5.0.tsv"

From 6c937316f86e8d8d3cea41f116fb0c7acd3dd7c5 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Wed, 27 Aug 2025 11:34:18 -0300
Subject: [PATCH 806/887] Update nextflow.config to use samller dfam and pfam
 files in tests

---
 tests/nextflow.config | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/tests/nextflow.config b/tests/nextflow.config
index 66ddf0787..eef755fe4 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -24,6 +24,12 @@ process {
 }
 
 params {
+    dfam_hmm                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm"
+    dfam_h3f                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3f"
+    dfam_h3i                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3i"
+    dfam_h3m                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3m"
+    dfam_h3p                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3p"
+    pfam_file                   = "${pipelines_testdata_base_path}/testdata/human/Pfam-A.hmm.gz"
     fusioncatcher_ref           = "${projectDir}/assets"
     arriba_ref_blacklist        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
     arriba_ref_cytobands        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/cytobands_hg38_GRCh38_v2.5.0.tsv"

From 9c560e8554f7ce2ba4541349d588036132840dff Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Wed, 27 Aug 2025 11:35:06 -0300
Subject: [PATCH 807/887] Update nextflow.config

---
 tests/nextflow.config | 6 ++++++
 1 file changed, 6 insertions(+)

diff --git a/tests/nextflow.config b/tests/nextflow.config
index 66ddf0787..eef755fe4 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -24,6 +24,12 @@ process {
 }
 
 params {
+    dfam_hmm                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm"
+    dfam_h3f                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3f"
+    dfam_h3i                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3i"
+    dfam_h3m                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3m"
+    dfam_h3p                    = "${pipelines_testdata_base_path}/testdata/human/test_starfusion_dfam.hmm.h3p"
+    pfam_file                   = "${pipelines_testdata_base_path}/testdata/human/Pfam-A.hmm.gz"
     fusioncatcher_ref           = "${projectDir}/assets"
     arriba_ref_blacklist        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/blacklist_hg38_GRCh38_v2.5.0.tsv.gz"
     arriba_ref_cytobands        = "s3://nf-core-awsmegatests/rnafusion/references/GRCh38/arriba/2.5.0/cytobands_hg38_GRCh38_v2.5.0.tsv"

From e3e4c13ae5d6f5ad247325db4195559388b8a339 Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Wed, 27 Aug 2025 19:11:36 +0200
Subject: [PATCH 808/887] Update stub test snapshot

---
 tests/test_stub.nf.test.snap | 21 +++++++--------------
 1 file changed, 7 insertions(+), 14 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 61f9424c2..e389619f4 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -203,7 +203,6 @@
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "samtools",
                 "samtools/test.Aligned.sortedByCoord.bam",
-                "samtools/test.Aligned.sortedByCoord.bam.csi",
                 "samtools/test.flagstat",
                 "samtools/test.idxstats",
                 "samtools/test.stats",
@@ -237,7 +236,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-27T15:23:50.746792719"
+        "timestamp": "2025-08-27T18:45:45.33537438"
     },
     "stub test stringtie": {
         "content": [
@@ -365,7 +364,6 @@
                 "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
                 "samtools",
                 "samtools/test.Aligned.sortedByCoord.bam",
-                "samtools/test.Aligned.sortedByCoord.bam.csi",
                 "samtools/test.flagstat",
                 "samtools/test.idxstats",
                 "samtools/test.stats",
@@ -402,7 +400,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-27T15:25:28.858027686"
+        "timestamp": "2025-08-27T18:46:51.467658698"
     },
     "stub test arriba": {
         "content": [
@@ -530,7 +528,6 @@
                 "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
                 "samtools",
                 "samtools/test.Aligned.sortedByCoord.bam",
-                "samtools/test.Aligned.sortedByCoord.bam.csi",
                 "samtools/test.flagstat",
                 "samtools/test.idxstats",
                 "samtools/test.stats",
@@ -560,7 +557,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-27T15:16:09.411578571"
+        "timestamp": "2025-08-27T18:41:39.472865742"
     },
     "stub test fusioninspector": {
         "content": [
@@ -821,7 +818,6 @@
                 "references/GRCh38/hgnc/hgnc_complete_set.txt",
                 "samtools",
                 "samtools/test.Aligned.sortedByCoord.bam",
-                "samtools/test.Aligned.sortedByCoord.bam.csi",
                 "samtools/test.flagstat",
                 "samtools/test.idxstats",
                 "samtools/test.stats",
@@ -853,7 +849,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-27T15:34:06.043690619"
+        "timestamp": "2025-08-27T18:51:23.029137047"
     },
     "stub test salmon": {
         "content": [
@@ -1174,7 +1170,6 @@
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "samtools",
                 "samtools/test.Aligned.sortedByCoord.bam",
-                "samtools/test.Aligned.sortedByCoord.bam.csi",
                 "samtools/test.flagstat",
                 "samtools/test.idxstats",
                 "samtools/test.stats",
@@ -1204,7 +1199,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-27T15:19:10.863659919"
+        "timestamp": "2025-08-27T18:42:46.678568423"
     },
     "stub test fusionreport": {
         "content": [
@@ -1443,7 +1438,6 @@
                 "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
                 "samtools",
                 "samtools/test.Aligned.sortedByCoord.bam",
-                "samtools/test.Aligned.sortedByCoord.bam.csi",
                 "samtools/test.flagstat",
                 "samtools/test.idxstats",
                 "samtools/test.stats",
@@ -1477,7 +1471,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-27T15:28:20.168052482"
+        "timestamp": "2025-08-27T18:48:05.810728943"
     },
     "stub test fastp": {
         "content": [
@@ -2055,7 +2049,6 @@
                 "salmon/test_meta_info.json",
                 "samtools",
                 "samtools/test.Aligned.sortedByCoord.bam",
-                "samtools/test.Aligned.sortedByCoord.bam.csi",
                 "samtools/test.flagstat",
                 "samtools/test.idxstats",
                 "samtools/test.stats",
@@ -2098,6 +2091,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-27T15:15:02.845874526"
+        "timestamp": "2025-08-27T18:40:27.833068219"
     }
 }
\ No newline at end of file

From 8a9d1579d506c6ac7dad5f1e832d8e7b13983dae Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Wed, 27 Aug 2025 20:00:09 +0200
Subject: [PATCH 809/887] Replace modules in test for QC_WORKFLOW

---
 .../local/qc_workflow/test/main.nf.test       | 15 ++++++--
 .../local/qc_workflow/test/main.nf.test.snap  | 38 +------------------
 2 files changed, 14 insertions(+), 39 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index ae36becb6..2abb710e1 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -28,8 +28,8 @@ nextflow_workflow {
             }
 
             // Filter GTF to extract rRNA genes
-            run("GET_RRNA_TRANSCRIPTS") {
-                script "../../../../modules/local/get_rrna_transcript/main.nf"
+            run("BIOAWK") {
+                script "../../../../modules/nf-core/bioawk/main.nf"
                 process {
                     """
                     input[0] = Channel.of(
@@ -42,13 +42,22 @@ nextflow_workflow {
                 }
             }
 
+            run("AGAT_CONVERTGFF2BED") {
+                script "../../../../modules/nf-core/agat/convertgff2bed/main.nf"
+                process {
+                    """
+                    input[0] = BIOAWK.out.output
+                    """
+                }
+            }
+
 
             // Convert rRNA BED to interval list (the necessary file)
             run("GATK4_BEDTOINTERVALLIST") {
                 script "../../../../modules/nf-core/gatk4/bedtointervallist/main.nf"
                 process {
                     """
-                    input[0] = GET_RRNA_TRANSCRIPTS.out.bed
+                    input[0] = AGAT_CONVERTGFF2BED.out.bed
                     input[1] = Channel.of(
                         [
                             [id: 'chr22_dic'],
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index cdaa0cc8b..3542ffc8e 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -1,22 +1,4 @@
 {
-    "duplicate_metrics": {
-        "content": "651d8a4702f9f9871e94afbce3e50e34",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.711230835"
-    },
-    "versions": {
-        "content": [
-            "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.686297468"
-    },
     "QC_WORKFLOW - Homo sapiens chr22": {
         "content": [
             [
@@ -45,24 +27,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-22T13:19:46.99615"
-    },
-    "rnaseq_metrics": {
-        "content": "84a348c3735ed2f6c47f346eeed661f4",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.704632753"
-    },
-    "insertsize_metrics": {
-        "content": "160db81b19843c4d46fe74ac61f9f013",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2024-12-04T19:29:09.717929716"
+        "timestamp": "2025-08-27T19:55:15.893171257"
     }
 }
\ No newline at end of file

From 605cbc711fdd9b64a6a739e28e8f211592ce215b Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 28 Aug 2025 11:16:54 +0200
Subject: [PATCH 810/887] update qc workflow test and snap

---
 .../local/qc_workflow/test/main.nf.test       | 22 +++++++++++++------
 1 file changed, 15 insertions(+), 7 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index ae36becb6..c4db1c92f 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -27,31 +27,39 @@ nextflow_workflow {
                 }
             }
 
-            // Filter GTF to extract rRNA genes
-            run("GET_RRNA_TRANSCRIPTS") {
-                script "../../../../modules/local/get_rrna_transcript/main.nf"
+            run("BIOAWK") {
+                script "../../../../modules/nf-core/bioawk/main"
                 process {
                     """
                     input[0] = Channel.of(
                         [
-                            [id: 'fasta'],
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExist: true)
+                            [id: 'gtf'],
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
                         ]
                     )
                     """
                 }
             }
 
+            run("AGAT_CONVERTGFF2BED") {
+                script "../../../../modules/nf-core/agat/convertgff2bed/main"
+                process {
+                    """
+                    input[0] = BIOAWK.out.output
+                    """
+                }
+            }
+
 
             // Convert rRNA BED to interval list (the necessary file)
             run("GATK4_BEDTOINTERVALLIST") {
                 script "../../../../modules/nf-core/gatk4/bedtointervallist/main.nf"
                 process {
                     """
-                    input[0] = GET_RRNA_TRANSCRIPTS.out.bed
+                    input[0] = AGAT_CONVERTGFF2BED.out.bed
                     input[1] = Channel.of(
                         [
-                            [id: 'chr22_dic'],
+                            [id: 'chr22_dict'],
                             file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.dict", checkIfExist: true)
                         ]
                     )

From 225aeb999390a1341f877439fc8eff71fb2385cb Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Thu, 28 Aug 2025 09:09:18 -0300
Subject: [PATCH 811/887] Update workflows/rnafusion.nf

Co-authored-by: Anabella Trigila <18577080+atrigila@users.noreply.github.com>
---
 workflows/rnafusion.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index dbb5e726e..4043980bb 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -188,7 +188,6 @@ workflow RNAFUSION {
                 BUILD_REFERENCES.out.fasta,
                 [[:], []]
             )
-            FASTQ_ALIGN_STAR.out.bam_sorted_aligned.view()
             SAMTOOLS_INDEX(FASTQ_ALIGN_STAR.out.bam_sorted_aligned)
             ch_bam_bai = FASTQ_ALIGN_STAR.out.bam_sorted_aligned
                 .join(SAMTOOLS_INDEX.out.bai, failOnMismatch:true, failOnDuplicate:true)

From 88c99323add6dc1d8a238efb30a0da323ee921e0 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Thu, 28 Aug 2025 10:22:03 -0300
Subject: [PATCH 812/887] Update main.nf.test

---
 subworkflows/local/qc_workflow/test/main.nf.test | 15 ++++++++++++---
 1 file changed, 12 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index ae36becb6..2abb710e1 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -28,8 +28,8 @@ nextflow_workflow {
             }
 
             // Filter GTF to extract rRNA genes
-            run("GET_RRNA_TRANSCRIPTS") {
-                script "../../../../modules/local/get_rrna_transcript/main.nf"
+            run("BIOAWK") {
+                script "../../../../modules/nf-core/bioawk/main.nf"
                 process {
                     """
                     input[0] = Channel.of(
@@ -42,13 +42,22 @@ nextflow_workflow {
                 }
             }
 
+            run("AGAT_CONVERTGFF2BED") {
+                script "../../../../modules/nf-core/agat/convertgff2bed/main.nf"
+                process {
+                    """
+                    input[0] = BIOAWK.out.output
+                    """
+                }
+            }
+
 
             // Convert rRNA BED to interval list (the necessary file)
             run("GATK4_BEDTOINTERVALLIST") {
                 script "../../../../modules/nf-core/gatk4/bedtointervallist/main.nf"
                 process {
                     """
-                    input[0] = GET_RRNA_TRANSCRIPTS.out.bed
+                    input[0] = AGAT_CONVERTGFF2BED.out.bed
                     input[1] = Channel.of(
                         [
                             [id: 'chr22_dic'],

From d82593c854b7d6777f95ee46479011ba40f42f35 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Thu, 28 Aug 2025 10:22:57 -0300
Subject: [PATCH 813/887] Update main.nf.test.snap

---
 .../local/qc_workflow/test/main.nf.test.snap  | 40 ++-----------------
 1 file changed, 3 insertions(+), 37 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index cdaa0cc8b..a8f3b776c 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -1,22 +1,4 @@
 {
-    "duplicate_metrics": {
-        "content": "651d8a4702f9f9871e94afbce3e50e34",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.711230835"
-    },
-    "versions": {
-        "content": [
-            "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.686297468"
-    },
     "QC_WORKFLOW - Homo sapiens chr22": {
         "content": [
             [
@@ -45,24 +27,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-22T13:19:46.99615"
-    },
-    "rnaseq_metrics": {
-        "content": "84a348c3735ed2f6c47f346eeed661f4",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.704632753"
-    },
-    "insertsize_metrics": {
-        "content": "160db81b19843c4d46fe74ac61f9f013",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
+            "nextflow": "25.04.2"
         },
-        "timestamp": "2024-12-04T19:29:09.717929716"
+        "timestamp": "2025-08-27T19:55:15.893171257"
     }
-}
\ No newline at end of file
+}

From c59cd57d3c62d0dfbfb728def91c3511e5d7166b Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Thu, 28 Aug 2025 13:04:40 -0300
Subject: [PATCH 814/887] Update CHANGELOG.md

---
 CHANGELOG.md | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1837f0ef4..4cda7f669 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -91,6 +91,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
 - Modified `test_build` profile to use a reduced version of Pfam and Dfam files [#733](https://github.com/nf-core/rnafusion/pull/733)
 - Changed local `ARRIBA_VISUALIZATION`, `CTATSPLICING_STARTOCANCERINTRONS`, `CTATSPLICING_PREPGENOMELIB`, `FUSIONINSPECTOR`, `STARFUSION_DETECT` for its nf-core module versions [#740](https://github.com/nf-core/rnafusion/pull/740)
+- Changed local `FASTQ_ALIGN_STAR` for subworkflow from nf-core [#756](https://github.com/nf-core/rnafusion/pull/756)
 
 ### Fixed
 
@@ -115,6 +116,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update VCF_COLLECT script to adapt to transcript_version not being an entry in fusioninspector gtf anymore [#726](https://github.com/nf-core/rnafusion/pull/726)
 - Fix rRNA detection and make it more customizable with nf-core modules [#736](https://github.com/nf-core/rnafusion/pull/736)
 - Fix rRNA detection in GTF using `transcript_type` [#749](https://github.com/nf-core/rnafusion/pull/749)
+- Fixed nf-test for `QC_WORKFLOW` subworkflow [#756](https://github.com/nf-core/rnafusion/pull/756)
 
 ### Removed
 

From 66077fb283895e11b6b008c226c13ddf30d21152 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Thu, 28 Aug 2025 17:09:22 +0000
Subject: [PATCH 815/887] test: update snapshot

---
 .../local/qc_workflow/test/main.nf.test       | 15 ++++++--
 .../local/qc_workflow/test/main.nf.test.snap  | 36 +------------------
 2 files changed, 13 insertions(+), 38 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index ae36becb6..2abb710e1 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -28,8 +28,8 @@ nextflow_workflow {
             }
 
             // Filter GTF to extract rRNA genes
-            run("GET_RRNA_TRANSCRIPTS") {
-                script "../../../../modules/local/get_rrna_transcript/main.nf"
+            run("BIOAWK") {
+                script "../../../../modules/nf-core/bioawk/main.nf"
                 process {
                     """
                     input[0] = Channel.of(
@@ -42,13 +42,22 @@ nextflow_workflow {
                 }
             }
 
+            run("AGAT_CONVERTGFF2BED") {
+                script "../../../../modules/nf-core/agat/convertgff2bed/main.nf"
+                process {
+                    """
+                    input[0] = BIOAWK.out.output
+                    """
+                }
+            }
+
 
             // Convert rRNA BED to interval list (the necessary file)
             run("GATK4_BEDTOINTERVALLIST") {
                 script "../../../../modules/nf-core/gatk4/bedtointervallist/main.nf"
                 process {
                     """
-                    input[0] = GET_RRNA_TRANSCRIPTS.out.bed
+                    input[0] = AGAT_CONVERTGFF2BED.out.bed
                     input[1] = Channel.of(
                         [
                             [id: 'chr22_dic'],
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index cdaa0cc8b..4a95d91b4 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -1,22 +1,4 @@
 {
-    "duplicate_metrics": {
-        "content": "651d8a4702f9f9871e94afbce3e50e34",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.711230835"
-    },
-    "versions": {
-        "content": [
-            "versions.yml:md5,3f13b395c67e317f74194b3b6c89f139"
-        ],
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.686297468"
-    },
     "QC_WORKFLOW - Homo sapiens chr22": {
         "content": [
             [
@@ -47,22 +29,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-07-22T13:19:46.99615"
-    },
-    "rnaseq_metrics": {
-        "content": "84a348c3735ed2f6c47f346eeed661f4",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.704632753"
-    },
-    "insertsize_metrics": {
-        "content": "160db81b19843c4d46fe74ac61f9f013",
-        "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.10.2"
-        },
-        "timestamp": "2024-12-04T19:29:09.717929716"
+        "timestamp": "2025-08-28T17:08:08.334459479"
     }
 }
\ No newline at end of file

From b771b47d8df51a1e5c264f9d3e9d648d4fc72c3e Mon Sep 17 00:00:00 2001
From: delfiterradas <terradasdelfi@gmail.com>
Date: Thu, 28 Aug 2025 22:20:32 +0200
Subject: [PATCH 816/887] Update nf-test to avoid failure due to unstable file

---
 .../tests/main.nf.test                        |  3 +-
 .../tests/main.nf.test.snap                   | 32 +++----------------
 2 files changed, 7 insertions(+), 28 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
index cc579b57e..e02352e94 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
@@ -118,7 +118,8 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { assert snapshot(workflow.out).match() }
+                { assert snapshot(file(workflow.out.ch_arriba_visualisation[0][1]).length()) },
+                { assert snapshot(workflow.out.versions).match() }
             )
         }
     }
diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
index 7677e5b5f..8c2578116 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
@@ -36,37 +36,15 @@
     },
     "FUSIONINSPECTOR_WORKFLOW - human": {
         "content": [
-            {
-                "0": [
-                    [
-                        {
-                            "id": "test_sample"
-                        },
-                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,8264fb7e5cbf6aab0b7971953ddd9b87"
-                    ]
-                ],
-                "1": [
-                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
-                    "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
-                ],
-                "ch_arriba_visualisation": [
-                    [
-                        {
-                            "id": "test_sample"
-                        },
-                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,8264fb7e5cbf6aab0b7971953ddd9b87"
-                    ]
-                ],
-                "versions": [
-                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
-                    "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
-                ]
-            }
+            [
+                "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
+                "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
+            ]
         ],
         "meta": {
             "nf-test": "0.9.2",
             "nextflow": "25.04.2"
         },
-        "timestamp": "2025-08-26T20:05:51.190155915"
+        "timestamp": "2025-08-28T21:54:05.453749443"
     }
 }
\ No newline at end of file

From 1acb395a11e2f51c31ceb66a3517e6d6b8a3573b Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Thu, 28 Aug 2025 17:49:58 -0300
Subject: [PATCH 817/887] Update CHANGELOG.md

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1837f0ef4..89c1ef1f6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -49,6 +49,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Added support for references hosted on the nf-core AWS S3 bucket. [#717](https://github.com/nf-core/rnafusion/pull/717)
 - Added `--dfam_hmm`, `--dfam_h3f`, `--dfam_h3i`, `--dfam_h3m`, `--dfam_h3p`, `--pfam_file` and `--annot_filter_url` parameters to allow use of custom files in `STARFUSION_BUILD` module [#709](https://github.com/nf-core/rnafusion/pull/709)
 - Add nf-test to local module: `VCF_COLLECT`. [#745](https://github.com/nf-core/rnafusion/pull/745)
+- Added nf-test for local subworkflow: `FUSIONINSPECTOR_WORKFLOW`. [#753](https://github.com/nf-core/rnafusion/pull/753)
 
 ### Changed
 

From b8e59d4ad35ff07b57f5a82388ca6254f82af4fb Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Fri, 29 Aug 2025 09:53:02 -0300
Subject: [PATCH 818/887] Fix modules.json

---
 modules.json | 2 ++
 1 file changed, 2 insertions(+)

diff --git a/modules.json b/modules.json
index 96245f791..1833dcc8d 100644
--- a/modules.json
+++ b/modules.json
@@ -224,6 +224,8 @@
                     "fastq_align_star": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "installed_by": ["subworkflows"]
+                    },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
                         "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",

From 5e0383b85a0b69d19203693400794466141d9326 Mon Sep 17 00:00:00 2001
From: Delfina Terradas <155591053+delfiterradas@users.noreply.github.com>
Date: Fri, 29 Aug 2025 10:17:00 -0300
Subject: [PATCH 819/887] Fix main.nf.test.snap

---
 subworkflows/local/qc_workflow/test/main.nf.test.snap | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test.snap b/subworkflows/local/qc_workflow/test/main.nf.test.snap
index 7b7f8ee53..80a937268 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test.snap
+++ b/subworkflows/local/qc_workflow/test/main.nf.test.snap
@@ -27,6 +27,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
         "timestamp": "2025-08-28T17:08:08.334459479"
     }
-}
\ No newline at end of file
+}

From a3472084b4e2a1d63a2bd90e088d84c63c57cac2 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 1 Sep 2025 16:15:12 +0200
Subject: [PATCH 820/887] reshuffling

---
 subworkflows/local/fusioninspector_workflow/main.nf | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index 9d5711ad4..2c3213ccc 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -55,6 +55,11 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
             fusion_data = tsv_abridged_nonempty.combine(AGAT_CONVERTSPGFF2TSV.out.tsv, fusionreport_out, fusionreport_csv).ifEmpty { Channel.empty() }
+            fusion_data = tsv_abridged_nonempty
+                .combine(AGAT_CONVERTSPGFF2TSV.out.tsv)
+                .combine(fusionreport_out)
+                .combine(fusionreport_csv)
+                .ifEmpty{ Channel.empty() }
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)

From fcfe9b17302bac4038c8b3ba3cf361a4c47aeafa Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Mon, 1 Sep 2025 17:07:54 +0200
Subject: [PATCH 821/887] Update CHANGELOG.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 1a35d7776..c839ac19b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -89,7 +89,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Update logo [#715](https://github.com/nf-core/rnafusion/pull/715)
 - Replaced local `STARFUSION_BUILD` for module from nf-core [#709](https://github.com/nf-core/rnafusion/pull/709)
 - Modified `test_build` profile to use a reduced version of Pfam and Dfam files [#733](https://github.com/nf-core/rnafusion/pull/733)
-- Updated the documentation to reflect the changes done in the release [#741](https://github.com/nf-core/rnafusion/pull/741)
+- Updated the documentation to reflect the changes done in this release [#741](https://github.com/nf-core/rnafusion/pull/741)
 
 ### Fixed
 

From 021f5f8f2acc5beb2b6f1a4a69fd7bfa0c5e5765 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Mon, 1 Sep 2025 17:11:21 +0200
Subject: [PATCH 822/887] Update docs/output.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 docs/output.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index e8ad8fefd..a6b79f190 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -95,7 +95,7 @@ If no parameters are specified, the default is applied.
 
 ### Agat
 
-[Arriba](https://github.com/NBISweden/AGAT) is to convert the GFF file to a TSV file, which is then used in the VCF creation.
+[Agat](https://github.com/NBISweden/AGAT) is to convert the GFF file to a TSV file, which is then used in the VCF creation.
 
 <details markdown="1">
 <summary>Output files</summary>

From 23210771ed62159f992b7316636c3fa3bd30d67a Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Mon, 1 Sep 2025 17:11:32 +0200
Subject: [PATCH 823/887] Update docs/output.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 docs/output.md | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index a6b79f190..c1044c4d8 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -158,7 +158,9 @@ If `--tools ctatsplicing` is present, [CTAT-SPLICING](https://github.com/Trinity
 
 ### Fastp
 
-If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will filter low quality reads as well as bases at the 5' and 3' ends, trim adapters (automatically detected, but input with parameter `--adapter_fasta` is possible). 3' trimming is also possible via parameter `--trim_tail_fusioncatcher`.
+If `--tools fastp` is present, [fastp](https://github.com/OpenGene/fastp) will filter low quality reads as well as bases at the 5' and 3' ends, trim adapters (automatically detected, but input with parameter `--adapter_fasta` is possible). 3' trimming is also possible via parameter `--trim_tail`.
+
+As fusioncatcher is especially sensitive to read length, 100 bp being the recommended length, an additional parameter `--trim_tail_fusioncatcher` triggers an extra fastp process with 3' trimming of the length given, these triggered reads are then fed to fusioncatcher but the non-extra trimmed ones are still used for arriba and STAR-Fusion.
 
 <details markdown="1">
 <summary>Output files</summary>

From 71fb65278a8b72919c73a0c022ab427db5b780ee Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Mon, 1 Sep 2025 17:11:46 +0200
Subject: [PATCH 824/887] Update README.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 README.md | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 1645c0ca3..8187200b7 100644
--- a/README.md
+++ b/README.md
@@ -64,7 +64,8 @@ The pipeline is also able to build the references in case files from a specific
 4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)
 5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)
 6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
-7. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
+7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)
+7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
 8. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)
 9. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)
 10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)

From 69a133cf66b58c638b6f2d1b1eca3344eca65640 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Mon, 1 Sep 2025 17:11:56 +0200
Subject: [PATCH 825/887] Update README.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 README.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/README.md b/README.md
index 8187200b7..84c11805d 100644
--- a/README.md
+++ b/README.md
@@ -71,6 +71,7 @@ The pipeline is also able to build the references in case files from a specific
 10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)
 11. Post-processing and analysis of data
     - [FusionInspector](https://github.com/FusionInspector/FusionInspector)
+    - Summarize information into a VCF file
     - [Arriba](https://github.com/suhrig/arriba) visualisation
     - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
 12. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))

From 9888efb024013f6324737fb30d09c66bff3dd19d Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
Date: Mon, 1 Sep 2025 17:12:14 +0200
Subject: [PATCH 826/887] Update docs/output.md

Co-authored-by: Annick Renevey <47788523+rannick@users.noreply.github.com>
---
 docs/output.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index c1044c4d8..8795bf6a6 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -399,4 +399,3 @@ Vcf-collect takes as input the results of fusion-report and fusioninspector. Tha
 
 </details>
 
-[Megafusion](https://github.com/J35P312/MegaFusion) converts RNA fusion files to SV VCF and collects statistics and metrics in a VCF file.

From f1b4a6f8990702f3da4ea899f364be2eb2691257 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 1 Sep 2025 17:12:59 +0200
Subject: [PATCH 827/887] add missing links

---
 README.md     | 2 +-
 docs/usage.md | 2 +-
 2 files changed, 2 insertions(+), 2 deletions(-)

diff --git a/README.md b/README.md
index 1645c0ca3..b47b62498 100644
--- a/README.md
+++ b/README.md
@@ -54,7 +54,7 @@ The pipeline is also able to build the references in case files from a specific
 8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs
 
 > [!WARNING]
-> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source.
+> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source. See the [References](#references) section for more information.
 
 #### Main workflow
 
diff --git a/docs/usage.md b/docs/usage.md
index d05e4f6f9..df2a100d6 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -10,7 +10,7 @@ The pipeline is divided into two parts:
 
 1. Download and build references
 
-- Download the references from the S3 bucket (recommended) or build them with the pipeline
+- [Download](#download-and-build-references) the references from the S3 bucket (recommended) or build them with the pipeline
 - **Important**: has to be done with each new release
 
 2. Running the analysis

From ada6a0d5867b2c116eac5958d737b0b80dbcef89 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Mon, 1 Sep 2025 17:20:35 +0200
Subject: [PATCH 828/887] apply suggestions from comment

---
 conf/modules.config | 14 ++++++++++++++
 docs/output.md      | 10 +++++++---
 2 files changed, 21 insertions(+), 3 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index e15c3914d..013814cce 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -139,6 +139,14 @@ process {
         ]
     }
 
+    withName: 'GATK4_CREATESEQUENCEDICTIONARY' {
+        publishDir = [
+            path: {  "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
+        ]
+    }
+
     withName: 'GATK4_MARKDUPLICATES' {
         publishDir = [
             path: { "${params.outdir}/picard" },
@@ -147,6 +155,12 @@ process {
         ]
     }
 
+    withName: 'GET_RRNA_TRANSCRIPTS' {
+        publishDir = [
+            enabled: false
+        ]
+    }
+
     withName: 'GFFREAD' {
         ext.args = { '-w -S' }
         publishDir = [
diff --git a/docs/output.md b/docs/output.md
index 8795bf6a6..9f3a0dd20 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -36,11 +36,17 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 - `<genomes_base>/`
   - `<genome>/`
-    - `fusion_report_db`
+    - `arriba/`
+      - `blacklist_hg38_GRCh38_v<gencode_version>.tsv.gz`
+      - `cytobands_hg38_GRCh38_v<gencode_version>.tsv`
+      - `known_fusions_hg38_GRCh38_v<gencode_version>.tsv.gz`
+      - `protein_domains_hg38_GRCh38_v<gencode_version>.gff3`
+    - `fusion_report_db/`
       - `cosmic.db`
       - `fusiongdb2.db`
       - `mitelman.db`
     - `gencode_v<gencode_version>/`
+      - `fusioncatcher/`
       - `gencode/`
         - `Homo_sapiens.GRCh38.<gencode_version>.all.fa`
         - `Homo_sapiens.GRCh38.<gencode_version>.cdna.all.fa.gz`
@@ -78,8 +84,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 ├── fusioncatcher
 ├── fusioninspector
 ├── fusionreport
-├── gatk4
-├── get
 ├── multiqc
 ├── picard
 ├── pipeline_info

From 52b43dd0f279d2412ef00f5ff8ef5a4d4498204d Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 2 Sep 2025 07:52:16 +0000
Subject: [PATCH 829/887] chore: replace local for nf-core subworkflow

---
 modules.json                                  |  9 ++-
 subworkflows/local/stringtie_workflow/main.nf | 36 ----------
 .../stringtie_workflow/tests/main.nf.test     | 70 -------------------
 .../tests/main.nf.test.snap                   | 56 ---------------
 .../nf-core/bam_stringtie_merge/main.nf       | 35 ++++++++++
 .../nf-core/bam_stringtie_merge/meta.yml      | 48 +++++++++++++
 .../bam_stringtie_merge/tests/main.nf.test    | 38 ++++++++++
 .../tests/main.nf.test.snap                   | 27 +++++++
 workflows/rnafusion.nf                        |  6 +-
 9 files changed, 158 insertions(+), 167 deletions(-)
 delete mode 100644 subworkflows/local/stringtie_workflow/main.nf
 delete mode 100644 subworkflows/local/stringtie_workflow/tests/main.nf.test
 delete mode 100644 subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
 create mode 100644 subworkflows/nf-core/bam_stringtie_merge/main.nf
 create mode 100644 subworkflows/nf-core/bam_stringtie_merge/meta.yml
 create mode 100644 subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test.snap

diff --git a/modules.json b/modules.json
index 1833dcc8d..c3555ee44 100644
--- a/modules.json
+++ b/modules.json
@@ -190,12 +190,12 @@
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": ["bam_stringtie_merge", "modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["modules"]
+                        "installed_by": ["bam_stringtie_merge", "modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
@@ -221,6 +221,11 @@
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["bam_sort_stats_samtools"]
                     },
+                    "bam_stringtie_merge": {
+                        "branch": "master",
+                        "git_sha": "5ce6cef7d4a100409aa30bca79b5020b6738767c",
+                        "installed_by": ["subworkflows"]
+                    },
                     "fastq_align_star": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
diff --git a/subworkflows/local/stringtie_workflow/main.nf b/subworkflows/local/stringtie_workflow/main.nf
deleted file mode 100644
index 5d6d72fc7..000000000
--- a/subworkflows/local/stringtie_workflow/main.nf
+++ /dev/null
@@ -1,36 +0,0 @@
-include { STRINGTIE_STRINGTIE }    from '../../../modules/nf-core/stringtie/stringtie/main'
-include { STRINGTIE_MERGE }        from '../../../modules/nf-core/stringtie/merge/main'
-
-
-workflow STRINGTIE_WORKFLOW {
-    take:
-        bam_sorted // channel: [meta, bam]
-        ch_chrgtf  // channel: [meta, gtf]
-
-    main:
-        ch_versions = Channel.empty()
-        ch_stringtie_gtf = Channel.empty()
-
-        STRINGTIE_STRINGTIE(
-            bam_sorted,
-            ch_chrgtf.map { _meta, gtf -> [ gtf ]}
-        )
-        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-        STRINGTIE_STRINGTIE
-            .out
-            .transcript_gtf
-            .map { it -> it[1] }
-            .set { stringtie_gtf }
-        ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
-
-
-        STRINGTIE_MERGE (stringtie_gtf, ch_chrgtf.map { _meta, gtf -> [ gtf ]})
-        ch_versions = ch_versions.mix(STRINGTIE_MERGE.out.versions)
-        ch_stringtie_gtf = STRINGTIE_MERGE.out.gtf
-
-    emit:
-        stringtie_gtf = ch_stringtie_gtf.ifEmpty(null) // channel: [meta, gtf]
-        versions      = ch_versions                    // channel: [ path(versions.yml) ]
-
-    }
diff --git a/subworkflows/local/stringtie_workflow/tests/main.nf.test b/subworkflows/local/stringtie_workflow/tests/main.nf.test
deleted file mode 100644
index da423ed57..000000000
--- a/subworkflows/local/stringtie_workflow/tests/main.nf.test
+++ /dev/null
@@ -1,70 +0,0 @@
-nextflow_workflow {
-
-    name "Test STRINGTIE_WORKFLOW"
-    script "../main.nf"
-    workflow "STRINGTIE_WORKFLOW"
-    tag "subworkflows"
-    tag "subworkflows_nfcore"
-    tag "stringtie"
-
-    test("Should run stringtie workflow with stringtie = true") {
-
-        when {
-            workflow {
-                """
-                input[0] = Channel.of([
-                        [ id:'test', strandedness:'reverse' ], // meta map
-                    file("${params.test_data_base}/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
-                ])
-                input[1] = Channel.of([
-                    [ id:'test' ], // meta map
-                    file("${params.test_data_base}/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
-                ])
-                """
-            }
-            params {
-                stringtie = true
-                outdir = "$outputDir"
-                test_data_base = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data"
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(workflow.out).match() },
-            )
-        }
-    }
-
-    test("Should run stringtie workflow with params.all = true") {
-
-        when {
-            workflow {
-                """
-                input[0] = Channel.of([
-                        [ id:'test', strandedness:'reverse' ], // meta map
-                    file("${params.test_data_base}/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
-                ])
-                input[1] = Channel.of([
-                    [ id:'test' ], // meta map
-                    file("${params.test_data_base}/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
-                ])
-                """
-            }
-            params {
-                all = true
-                outdir = "$outputDir"
-                test_data_base = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data"
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(workflow.out).match() },
-            )
-        }
-    }
-
-}
diff --git a/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap b/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
deleted file mode 100644
index 2abc99913..000000000
--- a/subworkflows/local/stringtie_workflow/tests/main.nf.test.snap
+++ /dev/null
@@ -1,56 +0,0 @@
-{
-    "Should run stringtie workflow with params.all = true": {
-        "content": [
-            {
-                "0": [
-                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
-                ],
-                "1": [
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
-                ],
-                "stringtie_gtf": [
-                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
-                ],
-                "versions": [
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-16T12:58:20.587179"
-    },
-    "Should run stringtie workflow with stringtie = true": {
-        "content": [
-            {
-                "0": [
-                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
-                ],
-                "1": [
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
-                ],
-                "stringtie_gtf": [
-                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
-                ],
-                "versions": [
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,0abbefd068f245608a323d26fcaf6d0b",
-                    "versions.yml:md5,be2acf8efb04dc33562c9d00df7c3a50"
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-07-16T12:33:08.507844"
-    }
-}
\ No newline at end of file
diff --git a/subworkflows/nf-core/bam_stringtie_merge/main.nf b/subworkflows/nf-core/bam_stringtie_merge/main.nf
new file mode 100644
index 000000000..7cbd582ed
--- /dev/null
+++ b/subworkflows/nf-core/bam_stringtie_merge/main.nf
@@ -0,0 +1,35 @@
+include { STRINGTIE_STRINGTIE } from '../../../modules/nf-core/stringtie/stringtie/main'
+include { STRINGTIE_MERGE    } from '../../../modules/nf-core/stringtie/merge/main'
+
+
+workflow BAM_STRINGTIE_MERGE {
+
+    take:
+    bam_sorted // channel: [ meta, bam ]
+    ch_chrgtf  // channel: [ meta, gtf ]
+
+    main:
+    ch_versions       = Channel.empty()
+    ch_stringtie_gtfs = Channel.empty()
+
+    STRINGTIE_STRINGTIE(
+        bam_sorted,
+        ch_chrgtf.map { _meta, gtf -> [ gtf ] }
+    )
+    ch_versions = ch_versions.mix(STRINGTIE_STRINGTIE.out.versions)
+
+    STRINGTIE_STRINGTIE.out.transcript_gtf
+        .map { it[1] }
+        .set { stringtie_gtfs }
+
+    STRINGTIE_MERGE(
+        stringtie_gtfs,
+        ch_chrgtf.map { _meta, gtf -> [ gtf ] }
+    )
+    ch_versions       = ch_versions.mix(STRINGTIE_MERGE.out.versions)
+    ch_stringtie_gtfs = STRINGTIE_MERGE.out.gtf
+
+    emit:
+    stringtie_gtf = ch_stringtie_gtfs // channel: [ meta, gtf ]
+    versions      = ch_versions                     // channel: [ path(versions.yml) ]
+}
diff --git a/subworkflows/nf-core/bam_stringtie_merge/meta.yml b/subworkflows/nf-core/bam_stringtie_merge/meta.yml
new file mode 100644
index 000000000..19c1496bf
--- /dev/null
+++ b/subworkflows/nf-core/bam_stringtie_merge/meta.yml
@@ -0,0 +1,48 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json
+name: "bam_stringtie_merge"
+description: >
+  Assemble transcripts from sorted BAM alignments using StringTie, then merge
+  per-sample transcript GTFs into a unified annotation.
+keywords:
+  - rna-seq
+  - transcript-assembly
+  - transcript-merge
+  - stringtie
+  - gtf
+components:
+  - stringtie/stringtie
+  - stringtie/merge
+input:
+  - bam_sorted:
+      type: file
+      description: |
+        Sorted alignment files.
+        Structure: [ val(meta), path(bam) ]
+      pattern: "*.bam"
+  - ch_chrgtf:
+      type: file
+      description: |
+        Reference gene annotation (GTF) for guided assembly and merge.
+        Structure: [ val(meta), path(gtf) ]
+      pattern: "*.gtf"
+output:
+  - stringtie_gtf:
+      type: file
+      description: |
+        Merged transcript annotation produced by StringTie --merge.
+        Structure: [ val(meta), path(gtf) ]
+      pattern: "*.gtf"
+  - versions:
+      type: file
+      description: |
+        File containing software versions for the subworkflow components.
+        Structure: [ path(versions.yml) ]
+      pattern: "versions.yml"
+authors:
+  - "@atrigila"
+  - "@rannick"
+  - "@nvnieuwk"
+maintainers:
+  - "@atrigila"
+  - "@rannick"
+  - "@nvnieuwk"
diff --git a/subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test b/subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test
new file mode 100644
index 000000000..48408633d
--- /dev/null
+++ b/subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test
@@ -0,0 +1,38 @@
+nextflow_workflow {
+
+    name "Test BAM_STRINGTIE_MERGE"
+    script "../main.nf"
+    workflow "BAM_STRINGTIE_MERGE"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "stringtie"
+    tag "stringtie/stringtie"
+    tag "stringtie/merge"
+    tag "subworkflows/bam_stringtie_merge"
+
+    test("Should run stringtie subworkflow") {
+
+        when {
+            workflow {
+                """
+                input[0] = Channel.of([
+                        [ id:'test', strandedness:'reverse' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam", checkIfExists: true)
+                ])
+                input[1] = Channel.of([
+                    [ id:'test' ], // meta map
+                    file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
+                ])
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() },
+            )
+        }
+    }
+
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test.snap b/subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test.snap
new file mode 100644
index 000000000..f671dd0b0
--- /dev/null
+++ b/subworkflows/nf-core/bam_stringtie_merge/tests/main.nf.test.snap
@@ -0,0 +1,27 @@
+{
+    "Should run stringtie subworkflow": {
+        "content": [
+            {
+                "0": [
+                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+                ],
+                "1": [
+                    "versions.yml:md5,3ab29dfb260f355cad809acd8dbd1424",
+                    "versions.yml:md5,7c84e875854527473ba6ada66b51e4a6"
+                ],
+                "stringtie_gtf": [
+                    "stringtie.merged.gtf:md5,6da479298d73d5b3216d4e1576a2bdf4"
+                ],
+                "versions": [
+                    "versions.yml:md5,3ab29dfb260f355cad809acd8dbd1424",
+                    "versions.yml:md5,7c84e875854527473ba6ada66b51e4a6"
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-08-31T23:17:24.40243201"
+    }
+}
\ No newline at end of file
diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index afbfa37d7..eb792032a 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -9,7 +9,7 @@ include { CAT_FASTQ                     }   from '../modules/nf-core/cat/fastq/m
 include { FASTQ_FASTQC_UMITOOLS_FASTP   }   from '../subworkflows/nf-core/fastq_fastqc_umitools_fastp/main'
 include { QC_WORKFLOW                   }   from '../subworkflows/local/qc_workflow'
 include { STARFUSION_DETECT             }   from '../modules/nf-core/starfusion/detect/main'
-include { STRINGTIE_WORKFLOW            }   from '../subworkflows/local/stringtie_workflow/main'
+include { BAM_STRINGTIE_MERGE           }   from '../subworkflows/nf-core/bam_stringtie_merge/main'
 include { FUSIONCATCHER_WORKFLOW        }   from '../subworkflows/local/fusioncatcher_workflow'
 include { FUSIONINSPECTOR_WORKFLOW      }   from '../subworkflows/local/fusioninspector_workflow'
 include { FUSIONREPORT_DETECT           }   from '../modules/nf-core/fusionreport/detect/main'
@@ -312,11 +312,11 @@ workflow RNAFUSION {
         //
 
         if(tools.contains("stringtie")) {
-            STRINGTIE_WORKFLOW (
+            BAM_STRINGTIE_MERGE (
                 ch_aligned_reads.map { meta, bam, _bai -> [meta, bam]},
                 BUILD_REFERENCES.out.gtf
             )
-            ch_versions = ch_versions.mix(STRINGTIE_WORKFLOW.out.versions)
+            ch_versions = ch_versions.mix(BAM_STRINGTIE_MERGE.out.versions)
         }
 
         //

From d43cf513eced86b22f186a40eeeebd8acc30b4c2 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 2 Sep 2025 10:20:39 +0200
Subject: [PATCH 830/887] remove baseDir in rrna transcript

---
 modules/local/get_rrna_transcript/main.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/modules/local/get_rrna_transcript/main.nf b/modules/local/get_rrna_transcript/main.nf
index 901c17b42..79b544791 100644
--- a/modules/local/get_rrna_transcript/main.nf
+++ b/modules/local/get_rrna_transcript/main.nf
@@ -20,9 +20,9 @@ process GET_RRNA_TRANSCRIPTS {
 
     script:
     """
-    $baseDir/bin/get_rrna_transcripts.py ${gtf} rrna.gtf
+    get_rrna_transcripts.py ${gtf} rrna.gtf
 
-    $baseDir/bin/gtf2bed rrna.gtf > rrna.bed
+    gtf2bed rrna.gtf > rrna.bed
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":

From 59fcd5438d0b5229b2b6db3a3a918d232a3ff302 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 2 Sep 2025 10:27:39 +0200
Subject: [PATCH 831/887] Update
 subworkflows/local/fusioninspector_workflow/main.nf

Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
---
 subworkflows/local/fusioninspector_workflow/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index 2c3213ccc..5e2ead7da 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -54,7 +54,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             AGAT_CONVERTSPGFF2TSV(gtf_nonempty)
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
-            fusion_data = tsv_abridged_nonempty.combine(AGAT_CONVERTSPGFF2TSV.out.tsv, fusionreport_out, fusionreport_csv).ifEmpty { Channel.empty() }
+            fusion_data = tsv_abridged_nonempty.combine(AGAT_CONVERTSPGFF2TSV.out.tsv, fusionreport_out, fusionreport_csv)
             fusion_data = tsv_abridged_nonempty
                 .combine(AGAT_CONVERTSPGFF2TSV.out.tsv)
                 .combine(fusionreport_out)

From 08c7dec29006e09fe074200976bf08ccab5d668f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 2 Sep 2025 10:27:45 +0200
Subject: [PATCH 832/887] Update
 subworkflows/local/fusioninspector_workflow/main.nf

Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com>
---
 subworkflows/local/fusioninspector_workflow/main.nf | 1 -
 1 file changed, 1 deletion(-)

diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index 5e2ead7da..4d3d61b9a 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -59,7 +59,6 @@ workflow FUSIONINSPECTOR_WORKFLOW {
                 .combine(AGAT_CONVERTSPGFF2TSV.out.tsv)
                 .combine(fusionreport_out)
                 .combine(fusionreport_csv)
-                .ifEmpty{ Channel.empty() }
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)

From ddd26f2084eefb563e75284b38d759e00124a530 Mon Sep 17 00:00:00 2001
From: atrigila <18577080+atrigila@users.noreply.github.com>
Date: Tue, 2 Sep 2025 08:33:13 +0000
Subject: [PATCH 833/887] chore: replace gtftorefflat for nf-core module

---
 CHANGELOG.md                                  |   1 +
 conf/modules.config                           |   4 +-
 .../local/uscs/custom_gtftogenepred/main.nf   |  39 ------
 subworkflows/local/build_references/main.nf   |   8 +-
 tests/test_build.nf.test.snap                 |  10 +-
 tests/test_stub.nf.test.snap                  | 113 ++++++++++--------
 tests/test_stub_bam.nf.test.snap              | 104 ++++++++--------
 7 files changed, 132 insertions(+), 147 deletions(-)
 delete mode 100644 modules/local/uscs/custom_gtftogenepred/main.nf

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f88ce94af..e995541cd 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -94,6 +94,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Changed local `ARRIBA_VISUALIZATION`, `CTATSPLICING_STARTOCANCERINTRONS`, `CTATSPLICING_PREPGENOMELIB`, `FUSIONINSPECTOR`, `STARFUSION_DETECT` for its nf-core module versions [#740](https://github.com/nf-core/rnafusion/pull/740)
 - Replaced local subworkflow `TRIM_WORKFLOW` for its nf-core subworkflow equivalent `FASTQ_FASTQC_UMITOOLS_FASTP` [#752](https://github.com/nf-core/rnafusion/pull/752)
 - Changed local `FASTQ_ALIGN_STAR` for subworkflow from nf-core [#756](https://github.com/nf-core/rnafusion/pull/756)
+- Replaced local subworkflow `STRINGTIE_WORKFLOW` for its nf-core version `BAM_STRINGTIE_MERGE` and the local module module `GTF_TO_REFFLAT` for nf-core's `UCSC_GTFTOGENEPRED` [#758](https://github.com/nf-core/rnafusion/pull/758)
 
 ### Fixed
 
diff --git a/conf/modules.config b/conf/modules.config
index da5569f14..517209790 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -166,8 +166,8 @@ process {
         ]
     }
 
-    withName: 'GTF_TO_REFFLAT' {
-        ext.args = "-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons"
+    withName: 'UCSC_GTFTOGENEPRED' {
+        ext.args = '-genePredExt -geneNameAsName2 -ignoreGroupsWithoutExons'
         publishDir = [
             path: {  "${params.genomes_base}/GRCh38/gencode_v${params.genome_gencode_version}/gencode" },
             mode: params.publish_dir_mode,
diff --git a/modules/local/uscs/custom_gtftogenepred/main.nf b/modules/local/uscs/custom_gtftogenepred/main.nf
deleted file mode 100644
index 9cc15765e..000000000
--- a/modules/local/uscs/custom_gtftogenepred/main.nf
+++ /dev/null
@@ -1,39 +0,0 @@
-process GTF_TO_REFFLAT {
-    tag "$meta.id"
-    label 'process_low'
-
-    conda "bioconda::ucsc-gtftogenepred=377"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ucsc-gtftogenepred:377--ha8a8165_5' :
-        'quay.io/biocontainers/ucsc-gtftogenepred:377--ha8a8165_5' }"
-
-    input:
-    tuple val(meta), path (gtf)
-
-    output:
-    path('*.refflat')    , emit: refflat
-    path "versions.yml"  , emit: versions
-
-    script:
-    def genepred = gtf + '.genepred'
-    def refflat = gtf + '.refflat'
-    """
-    gtfToGenePred -genePredExt -geneNameAsName2 ${gtf} ${genepred}
-    paste ${genepred} ${genepred} | cut -f12,16-25 > ${refflat}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gtfToGenePred: 377
-    END_VERSIONS
-    """
-
-    stub:
-    def refflat = gtf + '.refflat'
-    """
-    touch ${refflat}
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gtfToGenePred: 377
-    END_VERSIONS
-    """
-}
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index a7e9e859a..7ac3285a2 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -6,13 +6,13 @@
 
 include { GENCODE_DOWNLOAD }                from '../../../modules/local/gencode_download/main'
 include { HGNC_DOWNLOAD }                   from '../../../modules/local/hgnc/main'
-include { GTF_TO_REFFLAT }                  from '../../../modules/local/uscs/custom_gtftogenepred/main'
 
 /*
 ========================================================================================
     IMPORT NF-CORE MODULES/SUBWORKFLOWS
 ========================================================================================
 */
+include { UCSC_GTFTOGENEPRED              } from '../../../modules/nf-core/ucsc/gtftogenepred/main'
 include { CTATSPLICING_PREPGENOMELIB }      from '../../../modules/nf-core/ctatsplicing/prepgenomelib/main.nf'
 include { BIOAWK                          } from '../../../modules/nf-core/bioawk/main'
 include { AGAT_CONVERTGFF2BED             } from '../../../modules/nf-core/agat/convertgff2bed/main'
@@ -101,9 +101,9 @@ workflow BUILD_REFERENCES {
     def ch_refflat = Channel.empty()
     if (!params.skip_qc) {
         if (!exists_not_empty(params.refflat)){
-            GTF_TO_REFFLAT(ch_gtf)
-            ch_versions = ch_versions.mix(GTF_TO_REFFLAT.out.versions)
-            ch_refflat = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
+            UCSC_GTFTOGENEPRED(ch_gtf)
+            ch_versions = ch_versions.mix(UCSC_GTFTOGENEPRED.out.versions)
+            ch_refflat = UCSC_GTFTOGENEPRED.out.refflat.map { meta, rf -> [[id: meta.id], rf] }
         } else {
             ch_refflat = Channel.fromPath(params.refflat).map { that -> [[id:that.Name], that] }
         }
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 4bdf1c518..7e0e54ae2 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -24,9 +24,6 @@
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "HGNC_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
@@ -46,6 +43,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -70,6 +70,7 @@
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/minigenome.fa.fai",
                 "references/GRCh38/gencode_v46/gencode/minigenome.gtf.fasta",
+                "references/GRCh38/gencode_v46/gencode/minigenome.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/minigenome.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/minigenome.gtf.refflat",
                 "references/GRCh38/gencode_v46/salmon",
@@ -197,6 +198,7 @@
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
+                "minigenome.gtf.genepred:md5,c5b0d2118efd89f7a2831e2dc486b999",
                 "minigenome.gtf.interval_list:md5,6496748a0994cb8a9764761ae74f8004",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
@@ -285,6 +287,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T16:53:43.678975494"
+        "timestamp": "2025-09-02T08:18:01.248835367"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index dfe100279..5a3a1bc32 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -25,9 +25,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -70,6 +67,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -104,6 +104,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -234,9 +235,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:20:40.211602465"
+        "timestamp": "2025-09-02T08:22:03.749618742"
     },
     "stub test stringtie": {
         "content": [
@@ -264,9 +265,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -307,6 +305,9 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -341,6 +342,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -398,9 +400,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:21:21.812203485"
+        "timestamp": "2025-09-02T08:22:39.560551811"
     },
     "stub test arriba": {
         "content": [
@@ -431,9 +433,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -468,6 +467,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -505,6 +507,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -555,9 +558,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:15:00.863385937"
+        "timestamp": "2025-09-02T08:19:38.026180355"
     },
     "stub test fusioninspector": {
         "content": [
@@ -603,9 +606,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "HGNC_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
@@ -648,6 +648,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "VCF_COLLECT": {
                     "python": "3.11.0",
                     "vcf_collect": 0.1
@@ -716,6 +719,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -847,9 +851,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:31:32.043336569"
+        "timestamp": "2025-09-02T08:25:11.604461904"
     },
     "stub test salmon": {
         "content": [
@@ -876,9 +880,6 @@
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "SALMON_INDEX": {
                     "salmon": "1.10.3"
                 },
@@ -888,6 +889,9 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -914,6 +918,7 @@
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -944,7 +949,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:27:42.592742898"
+        "timestamp": "2025-09-02T08:24:28.067668122"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -978,9 +983,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -1020,6 +1022,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1066,6 +1071,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1197,9 +1203,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-27T18:42:46.678568423"
+        "timestamp": "2025-09-02T08:20:13.319176919"
     },
     "stub test fusionreport": {
         "content": [
@@ -1239,9 +1245,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -1284,6 +1287,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1339,6 +1345,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1469,9 +1476,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:26:22.470758896"
+        "timestamp": "2025-09-02T08:23:24.792491035"
     },
     "stub test fastp": {
         "content": [
@@ -1501,12 +1508,12 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1541,6 +1548,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1551,7 +1559,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T22:48:35.622133369"
+        "timestamp": "2025-09-02T08:23:56.858216733"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1578,12 +1586,12 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1613,6 +1621,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1623,7 +1632,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:16:52.604596162"
+        "timestamp": "2025-09-02T08:20:49.030393254"
     },
     "stub test fusioncatcher trim": {
         "content": [
@@ -1653,12 +1662,12 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1694,6 +1703,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1704,7 +1714,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:17:29.899160389"
+        "timestamp": "2025-09-02T08:21:15.319414382"
     },
     "stub test all": {
         "content": [
@@ -1768,9 +1778,6 @@
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "HGNC_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
@@ -1828,6 +1835,9 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "VCF_COLLECT": {
                     "python": "3.11.0",
                     "vcf_collect": 0.1
@@ -1922,6 +1932,7 @@
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -2089,8 +2100,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T22:48:04.457572169"
+        "timestamp": "2025-09-02T08:19:03.489568486"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 697d2e898..af94c528c 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -25,9 +25,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -50,6 +47,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -84,6 +84,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -189,9 +190,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T23:14:20.283916876"
+        "timestamp": "2025-09-02T08:29:13.77559568"
     },
     "stub test stringtie": {
         "content": [
@@ -219,9 +220,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -242,6 +240,9 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -276,6 +277,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -311,9 +313,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T23:15:30.482022054"
+        "timestamp": "2025-09-02T08:29:53.58622226"
     },
     "stub test arriba": {
         "content": [
@@ -344,9 +346,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -361,6 +360,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -398,6 +400,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -423,9 +426,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T22:48:22.193453934"
+        "timestamp": "2025-09-02T08:26:49.642086755"
     },
     "stub test fusioninspector": {
         "content": [
@@ -471,9 +474,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "HGNC_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
@@ -496,6 +496,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "VCF_COLLECT": {
                     "python": "3.11.0",
                     "vcf_collect": 0.1
@@ -564,6 +567,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -673,9 +677,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T23:30:41.022404222"
+        "timestamp": "2025-09-02T08:32:46.154112416"
     },
     "stub test salmon": {
         "content": [
@@ -706,9 +710,6 @@
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -724,6 +725,9 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -759,6 +763,7 @@
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -789,7 +794,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:45:12.252856442"
+        "timestamp": "2025-09-02T08:31:56.747683511"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -823,9 +828,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -845,6 +847,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -891,6 +896,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1000,9 +1006,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T23:06:10.100949608"
+        "timestamp": "2025-09-02T08:27:33.646044212"
     },
     "stub test fusionreport": {
         "content": [
@@ -1042,9 +1048,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -1067,6 +1070,9 @@
                     "samtools": 1.21,
                     "gawk": "5.1.0"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1122,6 +1128,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1227,9 +1234,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T23:22:51.682095887"
+        "timestamp": "2025-09-02T08:30:34.704355039"
     },
     "stub test fastp": {
         "content": [
@@ -1263,9 +1270,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -1275,6 +1279,9 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1318,6 +1325,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1328,7 +1336,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T22:51:21.946095021"
+        "timestamp": "2025-09-02T08:31:12.481382473"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1359,9 +1367,6 @@
                 "GENCODE_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "PICARD_COLLECTINSERTSIZEMETRICS": {
                     "picard": "3.3.0"
                 },
@@ -1371,6 +1376,9 @@
                 "SAMTOOLS_FAIDX": {
                     "samtools": 1.21
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "Workflow": {
                     "nf-core/rnafusion": "v4.0.0dev"
                 }
@@ -1409,6 +1417,7 @@
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1419,7 +1428,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-21T18:38:43.272605368"
+        "timestamp": "2025-09-02T08:28:10.779582543"
     },
     "stub test all": {
         "content": [
@@ -1483,9 +1492,6 @@
                 "GFFREAD": {
                     "gffread": "0.12.7"
                 },
-                "GTF_TO_REFFLAT": {
-                    "gtfToGenePred": 377
-                },
                 "HGNC_DOWNLOAD": {
                     "wget": "1.21.4"
                 },
@@ -1523,6 +1529,9 @@
                 "STRINGTIE_STRINGTIE": {
                     "stringtie": "2.2.3"
                 },
+                "UCSC_GTFTOGENEPRED": {
+                    "ucsc": 447
+                },
                 "VCF_COLLECT": {
                     "python": "3.11.0",
                     "vcf_collect": 0.1
@@ -1617,6 +1626,7 @@
                 "references/GRCh38/gencode_v46/gencode",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.fasta",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
@@ -1762,8 +1772,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T23:00:13.773945342"
+        "timestamp": "2025-09-02T08:26:08.667543202"
     }
 }
\ No newline at end of file

From ddbfd78cd1535ff7a7758755f304a64ed8d41cc4 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 2 Sep 2025 10:36:58 +0200
Subject: [PATCH 834/887] fix linting

---
 README.md              | 16 ++++++++--------
 docs/output.md         |  1 -
 docs/usage.md          |  2 +-
 ro-crate-metadata.json |  2 +-
 4 files changed, 10 insertions(+), 11 deletions(-)

diff --git a/README.md b/README.md
index 5a2b788eb..6da8f3b1a 100644
--- a/README.md
+++ b/README.md
@@ -64,18 +64,18 @@ The pipeline is also able to build the references in case files from a specific
 4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)
 5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)
 6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)
-7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)
-7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
-8. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)
-9. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)
-10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)
-11. Post-processing and analysis of data
+   7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)
+   7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
+7. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)
+8. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)
+9. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)
+10. Post-processing and analysis of data
     - [FusionInspector](https://github.com/FusionInspector/FusionInspector)
     - Summarize information into a VCF file
     - [Arriba](https://github.com/suhrig/arriba) visualisation
     - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))
-12. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
-13. Compress bam files to cram with [samtools view](http://www.htslib.org/)
+11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
+12. Compress bam files to cram with [samtools view](http://www.htslib.org/)
 
 ## Usage
 
diff --git a/docs/output.md b/docs/output.md
index 9f3a0dd20..9937f7fb4 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -402,4 +402,3 @@ The STAR index is generated with `--sjdbOverhang ${params.read_length - 1}`, `pa
 Vcf-collect takes as input the results of fusion-report and fusioninspector. That means fusions from all tools are aggregated. Fusioninspector applies a filter so it is possible some fusions detected by a caller are not filtered out by fusioninspector. In those cases, vcf-collect will display the fusions, but a lot of data will be missing as fusioninspector performs the analysis for each fusion.
 
 </details>
-
diff --git a/docs/usage.md b/docs/usage.md
index df2a100d6..d0cee2b1a 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -145,7 +145,7 @@ TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,forward
 | `sample`           | Custom sample name. This value needs to be unique across all entries in the samplesheet and cannot contain spaces                                                                                                                                                                                                                                                                                                                             | ✅       |
 | `strandedness`     | Strandedness: forward or reverse.                                                                                                                                                                                                                                                                                                                                                                                                             | ✅       |
 | `fastq_1`          | Full path to FastQ file for Illumina short reads 1. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`.                                                                                      | ❓       |
-| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`".                                                                                      | ❓       |
+| `fastq_2`          | Full path to FastQ file for Illumina short reads 2. File must exist, has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". It's recommended to always provide the FASTQ files because the pipeline will be able to create any missing files from these. The FASTQ files are required to run `salmon`, `fusioninspector` and `fusioncatcher`".                                                                                     | ❓       |
 | `bam`              | Full path to the BAM file created with STAR. File has to exist and must have the extension ".bam". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The BAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `cram` fields are empty.   | ❓       |
 | `bai`              | Full path to the index of the BAM file. File has to exist and must have the extension ".bai".                                                                                                                                                                                                                                                                                                                                                 | ❌       |
 | `cram`             | Full path to the CRAM file created with STAR. File has to exist and must have the extension ".cram". It's the responsibility of the pipeline user to make sure this file has been correctly created, see the [prepare chapter](#preparing-bamcramjunctionssplice_junctions) for more information. The CRAM file is required to run `ctatsplicing`, `stringtie`, `fusioninspector` and `arriba` when the `fastq_1` and `bam` fields are empty. | ❓       |
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 68b7f82a0..dcaae2ca0 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n7. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n8. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n11. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n12. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n13. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source. See the [References](#references) section for more information.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)\n7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n8. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n11. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - Summarize information into a VCF file\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n12. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n13. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"

From ea84891d6bb93de280131859681c54f00ad100ed Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 2 Sep 2025 10:40:06 +0200
Subject: [PATCH 835/887] fix config

---
 conf/modules.config | 6 ------
 1 file changed, 6 deletions(-)

diff --git a/conf/modules.config b/conf/modules.config
index 1d75baf17..066bd4813 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -165,12 +165,6 @@ process {
         ]
     }
 
-    withName: 'GET_RRNA_TRANSCRIPTS' {
-        publishDir = [
-            enabled: false
-        ]
-    }
-
     withName: 'GFFREAD' {
         ext.args = { '-w -S' }
         publishDir = [

From 36221f8e09cdfd86eec8c55eb8cb6af2564cfd58 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 2 Sep 2025 10:57:59 +0200
Subject: [PATCH 836/887] update

---
 subworkflows/local/fusioninspector_workflow/main.nf | 2 --
 1 file changed, 2 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index 2c3213ccc..8e1b3db2d 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -54,12 +54,10 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             AGAT_CONVERTSPGFF2TSV(gtf_nonempty)
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
-            fusion_data = tsv_abridged_nonempty.combine(AGAT_CONVERTSPGFF2TSV.out.tsv, fusionreport_out, fusionreport_csv).ifEmpty { Channel.empty() }
             fusion_data = tsv_abridged_nonempty
                 .combine(AGAT_CONVERTSPGFF2TSV.out.tsv)
                 .combine(fusionreport_out)
                 .combine(fusionreport_csv)
-                .ifEmpty{ Channel.empty() }
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)

From eeaf8bb77b223b1d6c22d526fd2d991ea77b9611 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Tue, 2 Sep 2025 11:03:35 +0200
Subject: [PATCH 837/887] fix lint

---
 ro-crate-metadata.json | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index dcaae2ca0..3925e8228 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source. See the [References](#references) section for more information.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)\n7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n8. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n9. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n10. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n11. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - Summarize information into a VCF file\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n12. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n13. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source. See the [References](#references) section for more information.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n   7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)\n   7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n7. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n8. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n9. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - Summarize information into a VCF file\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"

From e765480f93be36f8389536105f90902c30456cbe Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 2 Sep 2025 16:49:04 +0200
Subject: [PATCH 838/887] update snap

---
 tests/test_stub.nf.test.snap | 2066 ++++++++++++++++++++++++++++++++++
 1 file changed, 2066 insertions(+)
 create mode 100644 tests/test_stub.nf.test.snap

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
new file mode 100644
index 000000000..e472136c1
--- /dev/null
+++ b/tests/test_stub.nf.test.snap
@@ -0,0 +1,2066 @@
+{
+    "stub test starfusion": {
+        "content": [
+            27,
+            {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
+                    "bioawk": 1.0
+                },
+                "FASTQC_RAW": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
+                "GENCODE_DOWNLOAD": {
+                    "wget": "1.21.4"
+                },
+                "GTF_TO_REFFLAT": {
+                    "gtfToGenePred": 377
+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_FLAGSTAT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_IDXSTATS": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_INDEX": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_SORT": {
+                    "samtools": 1.21
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": 1.21
+                },
+                "STARFUSION_BUILD": {
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
+                },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
+                "STAR_ALIGN": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "STAR_GENOMEGENERATE": {
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38/hgnc",
+                "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
+                "references/GRCh38/hgnc/hgnc_complete_set.txt",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "samtools",
+                "samtools/test.Aligned.sortedByCoord.bam",
+                "samtools/test.flagstat",
+                "samtools/test.idxstats",
+                "samtools/test.stats",
+                "star",
+                "star/test.Aligned.sortedByCoord.bam.bai",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "star/test.Aligned.unsort.out.bam",
+                "star/test.Chimeric.out.junction",
+                "star/test.Log.final.out",
+                "star/test.Log.out",
+                "star/test.Log.progress.out",
+                "star/test.ReadsPerGene.out.tab",
+                "star/test.SJ.out.tab",
+                "star/test.Signal.UniqueMultiple.str1.out.bg",
+                "star/test.Signal.UniqueMultiple.str1.out.wig",
+                "star/test.out.sam",
+                "star/test.sortedByCoord.out.bam",
+                "star/test.tab",
+                "star/test.toTranscriptome.out.bam",
+                "star/test.unmapped_1.fastq.gz",
+                "star/test.unmapped_2.fastq.gz",
+                "star/testXd.out.bam",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
+                "stringtie/test.coverage.gtf",
+                "stringtie/test.gene.abundance.txt",
+                "stringtie/test.transcripts.gtf"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-09-02T15:53:38.903818"
+    }
+}
\ No newline at end of file

From 9dddc255031e5e205af98cf2c1eede46792b41e0 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 2 Sep 2025 17:07:49 +0200
Subject: [PATCH 839/887] udpate snap bam

---
 tests/test_stub_bam.nf.test.snap | 1739 ++++++++++++++++++++++++++++++
 1 file changed, 1739 insertions(+)
 create mode 100644 tests/test_stub_bam.nf.test.snap

diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
new file mode 100644
index 000000000..f2baf94f9
--- /dev/null
+++ b/tests/test_stub_bam.nf.test.snap
@@ -0,0 +1,1739 @@
+{
+    "stub test starfusion": {
+        "content": [
+            15,
+            {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
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+                },
+                "FASTQC_RAW": {
+                    "fastqc": "0.12.1"
+                },
+                "GATK4_BEDTOINTERVALLIST": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_CREATESEQUENCEDICTIONARY": {
+                    "gatk4": "4.6.1.0"
+                },
+                "GATK4_MARKDUPLICATES": {
+                    "gatk4": "4.6.1.0",
+                    "samtools": 1.21
+                },
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+                },
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+                },
+                "PICARD_COLLECTINSERTSIZEMETRICS": {
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+                },
+                "PICARD_COLLECTRNASEQMETRICS": {
+                    "picard": "3.3.0"
+                },
+                "SAMTOOLS_FAIDX": {
+                    "samtools": 1.21
+                },
+                "STARFUSION_BUILD": {
+                    "gunzip": 1.12,
+                    "hmmer": 3.4,
+                    "STAR-Fusion": "1.15.1"
+                },
+                "STARFUSION_DETECT": {
+                    "STAR-Fusion": "1.15.1"
+                },
+                "STAR_GENOMEGENERATE": {
+                    "star": "2.7.11b",
+                    "samtools": 1.21,
+                    "gawk": "5.1.0"
+                },
+                "Workflow": {
+                    "nf-core/rnafusion": "v4.0.0dev"
+                }
+            },
+            [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/GRCh38",
+                "references/GRCh38/gencode_v46",
+                "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
+                "references/GRCh38/gencode_v46/star",
+                "references/GRCh38/gencode_v46/star/Genome",
+                "references/GRCh38/gencode_v46/star/Log.out",
+                "references/GRCh38/gencode_v46/star/SA",
+                "references/GRCh38/gencode_v46/star/SAindex",
+                "references/GRCh38/gencode_v46/star/chrLength.txt",
+                "references/GRCh38/gencode_v46/star/chrName.txt",
+                "references/GRCh38/gencode_v46/star/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/star/chrStart.txt",
+                "references/GRCh38/gencode_v46/star/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/star/exonInfo.tab",
+                "references/GRCh38/gencode_v46/star/geneInfo.tab",
+                "references/GRCh38/gencode_v46/star/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/star/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/star/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/star/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/star/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/AnnotFilterRule.pm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/PFAM.domtblout.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_fusion_annot_lib.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/_prot_info_db.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/annotfiltrule_cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/blast_pairs.idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_gene_blast_pairs.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_pfam_dat.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/cp_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/fusion_annot_lib.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_pfam_hits.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/index_ref_annot_cdna.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/makeblastdb.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2.splice_bed.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/mm2_genome_idx.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.cdsplus.dfam_masked.fa.idx.ok",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.mini.sortu.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_annot.gtf.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome.fa.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/ref_genome_fai.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.cp.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/trans.blast.dat.index.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/__chkpts/validate_ctat_genome_lib.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.dat.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/blast_pairs.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.gz",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/fusion_annot_lib.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/pfam_domains.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdna.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cds",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.cdsplus.fa.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf.mm2.splice.bed",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.pep",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.mm2",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ndb",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nhr",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nin",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.njs",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.not",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nsq",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.ntf",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.nto",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Log.out",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm",
+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv"
+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-09-02T16:58:08.911034"
+    },
+    "stub test stringtie": {
+        "content": [
+            16,
+            {
+                "AGAT_CONVERTGFF2BED": {
+                    "agat": "v1.5.1"
+                },
+                "BIOAWK": {
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+                },
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+                }
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+            [
+                "agat",
+                "agat/Homo_sapiens.GRCh38.46.gtf.bed",
+                "bioawk",
+                "bioawk/Homo_sapiens.GRCh38.46.gtf_rrna.gtf.gz",
+                "fastqc",
+                "fastqc/test.html",
+                "fastqc/test.zip",
+                "gatk4",
+                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
+                "multiqc",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_report.html",
+                "picard",
+                "picard/test.bai",
+                "picard/test.bam",
+                "picard/test.bam.metrics",
+                "picard/test.cram",
+                "picard/test.cram.crai",
+                "picard/test.rna_metrics",
+                "picard/test_collectinsertsize.pdf",
+                "picard/test_collectinsertsize.txt",
+                "pipeline_info",
+                "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
+                "references",
+                "references/GRCh38",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt",
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+                "references/GRCh38/gencode_v46/starfusion/ctat_genome_lib_build_dir/trans.blast.dat.gz",
+                "references/GRCh38/hgnc",
+                "references/GRCh38/hgnc/HGNC-DB-timestamp.txt",
+                "references/GRCh38/hgnc/hgnc_complete_set.txt",
+                "salmon",
+                "salmon/test",
+                "salmon/test_lib_format_counts.json",
+                "salmon/test_meta_info.json",
+                "star",
+                "star/test.Aligned.sortedByCoord.out.bam",
+                "star/test.Aligned.sortedByCoord.out.bam.bai",
+                "starfusion",
+                "starfusion/test.starfusion.abridged.coding_effect.tsv",
+                "starfusion/test.starfusion.abridged.tsv",
+                "starfusion/test.starfusion.fusion_predictions.tsv",
+                "stringtie",
+                "stringtie/[:]",
+                "stringtie/[:]/stringtie.merged.gtf",
+                "stringtie/test.ballgown",
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+                "stringtie/test.gene.abundance.txt",
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+            ]
+        ],
+        "meta": {
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
+        },
+        "timestamp": "2025-09-02T16:51:37.37908"
+    }
+}
\ No newline at end of file

From e7c12b0529d1c2f65495bbb31680656bba3ac469 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 3 Sep 2025 09:41:43 +0200
Subject: [PATCH 840/887] change operator

---
 subworkflows/local/fusioninspector_workflow/main.nf | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index 8e1b3db2d..300840069 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -55,9 +55,9 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2TSV.out.versions)
 
             fusion_data = tsv_abridged_nonempty
-                .combine(AGAT_CONVERTSPGFF2TSV.out.tsv)
-                .combine(fusionreport_out)
-                .combine(fusionreport_csv)
+                .join(AGAT_CONVERTSPGFF2TSV.out.tsv)
+                .join(fusionreport_out)
+                .join(fusionreport_csv)
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)

From 34d147ee01dd380bc240d6d7f1710cbf076f9965 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 3 Sep 2025 10:03:49 +0200
Subject: [PATCH 841/887] Update docs/usage.md

---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index 607a2d43d..8fd045fd7 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -16,7 +16,7 @@ The pipeline is divided into two parts:
 
 2. Detecting fusions
 
-- Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, `StringTie` and `CTAT-SPLICING` (the last two are not supported by fusion-report and `StringTie` can be seen as more of an assembly tool, requires manual investigation).
+- Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, `StringTie` and `CTAT-SPLICING` (the last two are not supported by fusion-report and `StringTie` can be seen as more of an assembly tool that requires manual investigation).
 - QC: `Fastqc`, `MultiQC`, and `Picard CollectInsertSize`, `Picard CollectWgsMetrics`, `Picard Markduplicates`
 - Fusions visualization: `Arriba`, `fusion-report`, `FusionInspector`, and `vcf_collect`
 

From 11303bfc64e1c2ec1b21927aa9f7c09a791aee72 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 3 Sep 2025 12:26:14 +0200
Subject: [PATCH 842/887] udpate modules

---
 modules.json                                  |  48 +++----
 modules/nf-core/agat/convertspgff2tsv/main.nf |   6 +-
 .../nf-core/agat/convertspgff2tsv/meta.yml    |  26 ++--
 modules/nf-core/arriba/arriba/meta.yml        |  64 ++++++----
 .../nf-core/arriba/arriba/tests/main.nf.test  |  75 +++++++++++
 .../arriba/arriba/tests/main.nf.test.snap     |  20 +--
 .../arriba/arriba/tests/nextflow.config       |   3 +-
 modules/nf-core/cat/cat/meta.yml              |  23 ++--
 modules/nf-core/cat/fastq/meta.yml            |  18 +--
 modules/nf-core/fastp/main.nf                 |   6 +-
 modules/nf-core/fastp/meta.yml                |  79 +++++++-----
 .../nf-core/gatk4/bedtointervallist/main.nf   |  23 ++--
 .../nf-core/gatk4/bedtointervallist/meta.yml  |  24 ++--
 .../gatk4/createsequencedictionary/main.nf    |  25 ++--
 .../gatk4/createsequencedictionary/meta.yml   |  19 +--
 modules/nf-core/gatk4/markduplicates/main.nf  |  10 +-
 modules/nf-core/gatk4/markduplicates/meta.yml |  56 +++++----
 modules/nf-core/gffread/meta.yml              |  45 ++++---
 modules/nf-core/multiqc/environment.yml       |   2 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 .../nf-core/multiqc/tests/main.nf.test.snap   |  20 +--
 .../picard/collectinsertsizemetrics/meta.yml  |  23 ++--
 .../picard/collectrnaseqmetrics/meta.yml      |  48 ++++---
 .../nf-core/picard/collectwgsmetrics/meta.yml |  30 +++--
 modules/nf-core/salmon/index/meta.yml         |  36 +++---
 modules/nf-core/salmon/quant/meta.yml         |  76 +++++++-----
 modules/nf-core/samtools/convert/meta.yml     |  36 ++++--
 modules/nf-core/samtools/faidx/meta.yml       |  52 ++++----
 modules/nf-core/samtools/index/main.nf        |   2 +-
 modules/nf-core/samtools/index/meta.yml       |  28 +++--
 modules/nf-core/star/align/meta.yml           | 117 +++++++++++-------
 modules/nf-core/star/genomegenerate/meta.yml  |  18 +--
 .../starfusion/build/tests/main.nf.test       |  46 +++----
 modules/nf-core/stringtie/merge/meta.yml      |  43 ++++---
 modules/nf-core/stringtie/stringtie/meta.yml  |  42 ++++---
 modules/nf-core/ucsc/gtftogenepred/meta.yml   |  23 ++--
 .../local/fusioninspector_workflow/main.nf    |   2 +-
 37 files changed, 731 insertions(+), 487 deletions(-)

diff --git a/modules.json b/modules.json
index c3555ee44..fb18b3dc9 100644
--- a/modules.json
+++ b/modules.json
@@ -12,12 +12,12 @@
                     },
                     "agat/convertspgff2tsv": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
-                        "git_sha": "077818897bfe993e1ed91a0339bcbe53fe13edc7",
+                        "git_sha": "025a6f75e1f72e4ab60abb4bd65b3f289d4ad910",
                         "installed_by": ["modules"]
                     },
                     "arriba/visualisation": {
@@ -32,12 +32,12 @@
                     },
                     "cat/cat": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "ctatsplicing/prepgenomelib": {
@@ -54,7 +54,7 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
@@ -84,62 +84,62 @@
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
                         "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
                         "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
-                        "git_sha": "1ec937ab3edc307bc0d79a2200d784e9f0868359",
+                        "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
                         "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "a4488d3d09244f29fb4606ba4eef85d59dcc6ad8",
                         "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
-                        "git_sha": "980a4964be91da75fb3ccf576118a18e247f6d15",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
-                        "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "samtools/convert": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
@@ -154,7 +154,7 @@
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/sort": {
@@ -169,17 +169,17 @@
                     },
                     "star/align": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["fastq_align_star", "modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "starfusion/build": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
                         "installed_by": ["modules"]
                     },
                     "starfusion/detect": {
@@ -189,17 +189,17 @@
                     },
                     "stringtie/merge": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["bam_stringtie_merge", "modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["bam_stringtie_merge", "modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
                         "installed_by": ["modules"]
                     },
                     "umitools/extract": {
diff --git a/modules/nf-core/agat/convertspgff2tsv/main.nf b/modules/nf-core/agat/convertspgff2tsv/main.nf
index 772c7bd0b..d2b9b8608 100644
--- a/modules/nf-core/agat/convertspgff2tsv/main.nf
+++ b/modules/nf-core/agat/convertspgff2tsv/main.nf
@@ -18,13 +18,13 @@ process AGAT_CONVERTSPGFF2TSV {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
+    def args   = task.ext.args   ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     agat_convert_sp_gff2tsv.pl \\
-        --gff $gff \\
+        --gff ${gff} \\
         --output ${prefix}.tsv \\
-        $args
+        ${args}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/agat/convertspgff2tsv/meta.yml b/modules/nf-core/agat/convertspgff2tsv/meta.yml
index 31f098683..4c80ee890 100644
--- a/modules/nf-core/agat/convertspgff2tsv/meta.yml
+++ b/modules/nf-core/agat/convertspgff2tsv/meta.yml
@@ -27,21 +27,27 @@ input:
         type: file
         description: Annotation file in GFF3/GTF format
         pattern: "*.{gff, gtf}"
+        ontologies:
+          - edam: "http://edamontology.org/format_1975" # GFF3
+          - edam: "http://edamontology.org/format_2306" # GTF
 output:
-  - tsv:
-      - meta:
-          type: file
-          description: Annotation file in TSV format
-          pattern: "*.{gtf}"
+  tsv:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
       - "*.tsv":
           type: file
           description: Annotation file in TSV format
           pattern: "*.{gtf}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_2306 # GTF
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: "http://edamontology.org/format_3750" # YAML
 authors:
   - "@rannick"
 maintainers:
diff --git a/modules/nf-core/arriba/arriba/meta.yml b/modules/nf-core/arriba/arriba/meta.yml
index 91ba21039..967f83756 100644
--- a/modules/nf-core/arriba/arriba/meta.yml
+++ b/modules/nf-core/arriba/arriba/meta.yml
@@ -25,6 +25,7 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -34,6 +35,7 @@ input:
         type: file
         description: Assembly FASTA file
         pattern: "*.{fasta}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -43,25 +45,33 @@ input:
         type: file
         description: Annotation GTF file
         pattern: "*.{gtf}"
-  - - blacklist:
-        type: file
-        description: Blacklist file
-        pattern: "*.{tsv}"
-  - - known_fusions:
-        type: file
-        description: Known fusions file
-        pattern: "*.{tsv}"
-  - - cytobands:
-        type: file
-        description: Cytobands file
-        pattern: "*.{tsv}"
-  - - protein_domains:
-        type: file
-        description: Protein domains file
-        pattern: "*.{gff3}"
+        ontologies: []
+  - blacklist:
+      type: file
+      description: Blacklist file
+      pattern: "*.{tsv}"
+      ontologies:
+        - edam: http://edamontology.org/format_3475 # TSV
+  - known_fusions:
+      type: file
+      description: Known fusions file
+      pattern: "*.{tsv}"
+      ontologies:
+        - edam: http://edamontology.org/format_3475 # TSV
+  - cytobands:
+      type: file
+      description: Cytobands file
+      pattern: "*.{tsv}"
+      ontologies:
+        - edam: http://edamontology.org/format_3475 # TSV
+  - protein_domains:
+      type: file
+      description: Protein domains file
+      pattern: "*.{gff3}"
+      ontologies: []
 output:
-  - fusions:
-      - meta:
+  fusions:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -70,8 +80,9 @@ output:
           type: file
           description: File contains fusions which pass all of Arriba's filters.
           pattern: "*.{fusions.tsv}"
-  - fusions_fail:
-      - meta:
+          ontologies: []
+  fusions_fail:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -81,11 +92,14 @@ output:
           description: File contains fusions that Arriba classified as an artifact or
             that are also observed in healthy tissue.
           pattern: "*.{fusions.discarded.tsv}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@praveenraj2018"
   - "@rannick"
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test b/modules/nf-core/arriba/arriba/tests/main.nf.test
index e76c06871..b5f404134 100644
--- a/modules/nf-core/arriba/arriba/tests/main.nf.test
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test
@@ -13,6 +13,81 @@ nextflow_process {
     tag "star/genomegenerate"
     tag "star/align"
 
+    test("arriba - homo_sapiens - paired_end") {
+        config "./nextflow.config"
+
+        setup{
+            run("STAR_GENOMEGENERATE") {
+                script '../../../star/genomegenerate/main.nf'
+                process {
+                """
+                input[0] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[1] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                """
+                }
+            }
+
+            run("STAR_ALIGN") {
+                script '../../../star/align/main.nf'
+                process {
+                    """
+                    input[0] = Channel.of([
+                        [ id:'test', single_end:false ], // meta map
+                        [
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true),
+                            file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true)
+                        ]
+                    ])
+                    input[1] = STAR_GENOMEGENERATE.out.index
+                    input[2] = Channel.of([
+                        [ id:'test_gtf' ],
+                        [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                    ])
+                    input[3] = false
+                    input[4] = 'illumina'
+                    input[5] = false
+                    """
+                }
+            }
+        }
+
+        when {
+            process {
+                """
+                input[0] = STAR_ALIGN.out.bam
+                input[1] = Channel.of([
+                    [ id:'test_fasta' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ]
+                ])
+                input[2] = Channel.of([
+                    [ id:'test_gtf' ],
+                    [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ]
+                ])
+                input[3] = []
+
+                input[4] = []
+
+                input[5] = []
+
+                input[6] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(process.out).match() }
+            )
+        }
+    }
+
     test("arriba - homo_sapiens - paired_end - stub") {
 
         options "-stub"
diff --git a/modules/nf-core/arriba/arriba/tests/main.nf.test.snap b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
index 7cf286a2f..ad3e7aa9c 100644
--- a/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
+++ b/modules/nf-core/arriba/arriba/tests/main.nf.test.snap
@@ -52,7 +52,7 @@
         },
         "timestamp": "2025-07-04T12:35:18.703566"
     },
-    "homo_sapiens - paired_end - stub": {
+    "arriba - homo_sapiens - paired_end": {
         "content": [
             {
                 "0": [
@@ -61,7 +61,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                        "test.fusions.tsv:md5,7c3383f7eb6d79b84b0bd30a7ef02d70"
                     ]
                 ],
                 "1": [
@@ -70,11 +70,11 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                        "test.fusions.discarded.tsv:md5,445cb87a27f063e751f93498cf8d10b5"
                     ]
                 ],
                 "2": [
-                    "versions.yml:md5,d323796555db4a58fe4c6bc08d1dec30"
+                    "versions.yml:md5,d2eb9bb3bd4b21571a6aa2f39f1283af"
                 ],
                 "fusions": [
                     [
@@ -82,7 +82,7 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.fusions.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                        "test.fusions.tsv:md5,7c3383f7eb6d79b84b0bd30a7ef02d70"
                     ]
                 ],
                 "fusions_fail": [
@@ -91,18 +91,18 @@
                             "id": "test",
                             "single_end": false
                         },
-                        "test.fusions.discarded.tsv:md5,f50b84b1db4b83ba62ec1deacc69c260"
+                        "test.fusions.discarded.tsv:md5,445cb87a27f063e751f93498cf8d10b5"
                     ]
                 ],
                 "versions": [
-                    "versions.yml:md5,d323796555db4a58fe4c6bc08d1dec30"
+                    "versions.yml:md5,d2eb9bb3bd4b21571a6aa2f39f1283af"
                 ]
             }
         ],
         "meta": {
-            "nf-test": "0.9.0",
-            "nextflow": "24.04.4"
+            "nf-test": "0.9.2",
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2024-10-08T15:41:23.945072"
+        "timestamp": "2025-07-22T14:12:04.304647041"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/arriba/arriba/tests/nextflow.config b/modules/nf-core/arriba/arriba/tests/nextflow.config
index d3d5b00f6..60aac1728 100644
--- a/modules/nf-core/arriba/arriba/tests/nextflow.config
+++ b/modules/nf-core/arriba/arriba/tests/nextflow.config
@@ -1,7 +1,8 @@
 process {
     withName: STAR_GENOMEGENERATE {
-        ext.args = '--genomeSAindexNbases 11'
+        ext.args = '--genomeSAindexNbases 9'
     }
+
     withName: STAR_ALIGN {
         ext.args = '--readFilesCommand zcat --outSAMtype BAM Unsorted --outSAMunmapped Within --outBAMcompression 0 --outFilterMultimapNmax 50 --peOverlapNbasesMin 10 --alignSplicedMateMapLminOverLmate 0.5 --alignSJstitchMismatchNmax 5 -1 5 5 --chimSegmentMin 10 --chimOutType WithinBAM HardClip --chimJunctionOverhangMin 10 --chimScoreDropMax 30 --chimScoreJunctionNonGTAG 0 --chimScoreSeparation 1 --chimSegmentReadGapMax 3 --chimMultimapNmax 50'
     }
diff --git a/modules/nf-core/cat/cat/meta.yml b/modules/nf-core/cat/cat/meta.yml
index 81778a067..2a9284d7f 100644
--- a/modules/nf-core/cat/cat/meta.yml
+++ b/modules/nf-core/cat/cat/meta.yml
@@ -20,21 +20,24 @@ input:
         type: file
         description: List of compressed / uncompressed files
         pattern: "*"
+        ontologies: []
 output:
-  - file_out:
-      - meta:
-          type: file
-          description: Concatenated file. Will be gzipped if file_out ends with ".gz"
-          pattern: "${file_out}"
+  file_out:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
       - ${prefix}:
           type: file
           description: Concatenated file. Will be gzipped if file_out ends with ".gz"
           pattern: "${file_out}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@erikrikarddaniel"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/cat/fastq/meta.yml b/modules/nf-core/cat/fastq/meta.yml
index 91ff2fb5f..40d2f627c 100644
--- a/modules/nf-core/cat/fastq/meta.yml
+++ b/modules/nf-core/cat/fastq/meta.yml
@@ -21,9 +21,10 @@ input:
         type: file
         description: |
           List of input FastQ files to be concatenated.
+        ontologies: []
 output:
-  - reads:
-      - meta:
+  reads:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -32,11 +33,14 @@ output:
           type: file
           description: Merged fastq file
           pattern: "*.{merged.fastq.gz}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf
index 1342741d5..6e3e6c43a 100644
--- a/modules/nf-core/fastp/main.nf
+++ b/modules/nf-core/fastp/main.nf
@@ -48,7 +48,7 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> >(tee ${prefix}.fastp.log >&2) \\
+            2>| >(tee ${prefix}.fastp.log >&2) \\
         | gzip -c > ${prefix}.fastp.fastq.gz
 
         cat <<-END_VERSIONS > versions.yml
@@ -69,7 +69,7 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> >(tee ${prefix}.fastp.log >&2)
+            2>| >(tee ${prefix}.fastp.log >&2)
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
@@ -94,7 +94,7 @@ process FASTP {
             --thread $task.cpus \\
             --detect_adapter_for_pe \\
             $args \\
-            2> >(tee ${prefix}.fastp.log >&2)
+            2>| >(tee ${prefix}.fastp.log >&2)
 
         cat <<-END_VERSIONS > versions.yml
         "${task.process}":
diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml
index 9c4b24584..b8673272a 100644
--- a/modules/nf-core/fastp/meta.yml
+++ b/modules/nf-core/fastp/meta.yml
@@ -24,25 +24,27 @@ input:
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively. If you wish to run interleaved paired-end data,  supply as single-end data
           but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module.
-  - - adapter_fasta:
-        type: file
-        description: File in FASTA format containing possible adapters to remove.
-        pattern: "*.{fasta,fna,fas,fa}"
-  - - discard_trimmed_pass:
-        type: boolean
-        description: |
-          Specify true to not write any reads that pass trimming thresholds.
-          This can be used to use fastp for the output report only.
-  - - save_trimmed_fail:
-        type: boolean
-        description: Specify true to save files that failed to pass trimming thresholds
-          ending in `*.fail.fastq.gz`
-  - - save_merged:
-        type: boolean
-        description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz`
+        ontologies: []
+  - adapter_fasta:
+      type: file
+      description: File in FASTA format containing possible adapters to remove.
+      pattern: "*.{fasta,fna,fas,fa}"
+      ontologies: []
+  - discard_trimmed_pass:
+      type: boolean
+      description: |
+        Specify true to not write any reads that pass trimming thresholds.
+        This can be used to use fastp for the output report only.
+  - save_trimmed_fail:
+      type: boolean
+      description: Specify true to save files that failed to pass trimming thresholds
+        ending in `*.fail.fastq.gz`
+  - save_merged:
+      type: boolean
+      description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz`
 output:
-  - reads:
-      - meta:
+  reads:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -51,8 +53,10 @@ output:
           type: file
           description: The trimmed/modified/unmerged fastq reads
           pattern: "*fastp.fastq.gz"
-  - json:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  json:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -61,8 +65,10 @@ output:
           type: file
           description: Results in JSON format
           pattern: "*.json"
-  - html:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3464 # JSON
+  html:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -71,8 +77,9 @@ output:
           type: file
           description: Results in HTML format
           pattern: "*.html"
-  - log:
-      - meta:
+          ontologies: []
+  log:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -81,8 +88,9 @@ output:
           type: file
           description: fastq log file
           pattern: "*.log"
-  - reads_fail:
-      - meta:
+          ontologies: []
+  reads_fail:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -91,8 +99,10 @@ output:
           type: file
           description: Reads the failed the preprocessing
           pattern: "*fail.fastq.gz"
-  - reads_merged:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  reads_merged:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -101,11 +111,14 @@ output:
           type: file
           description: Reads that were successfully merged
           pattern: "*.{merged.fastq.gz}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@drpatelh"
   - "@kevinmenden"
diff --git a/modules/nf-core/gatk4/bedtointervallist/main.nf b/modules/nf-core/gatk4/bedtointervallist/main.nf
index 89960e04c..21e668c09 100644
--- a/modules/nf-core/gatk4/bedtointervallist/main.nf
+++ b/modules/nf-core/gatk4/bedtointervallist/main.nf
@@ -1,11 +1,11 @@
 process GATK4_BEDTOINTERVALLIST {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd'}"
 
     input:
     tuple val(meta), path(bed)
@@ -13,7 +13,7 @@ process GATK4_BEDTOINTERVALLIST {
 
     output:
     tuple val(meta), path('*.interval_list'), emit: interval_list
-    path  "versions.yml"                    , emit: versions
+    path "versions.yml",                      emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,18 +24,19 @@ process GATK4_BEDTOINTERVALLIST {
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK BedToIntervalList] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK BedToIntervalList] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         BedToIntervalList \\
-        --INPUT $bed \\
+        --INPUT ${bed} \\
         --OUTPUT ${prefix}.interval_list \\
-        --SEQUENCE_DICTIONARY $dict \\
+        --SEQUENCE_DICTIONARY ${dict} \\
         --TMP_DIR . \\
-        $args
+        ${args}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/gatk4/bedtointervallist/meta.yml b/modules/nf-core/gatk4/bedtointervallist/meta.yml
index 25348e162..b186132d0 100644
--- a/modules/nf-core/gatk4/bedtointervallist/meta.yml
+++ b/modules/nf-core/gatk4/bedtointervallist/meta.yml
@@ -26,6 +26,7 @@ input:
         type: file
         description: Input bed file
         pattern: "*.bed"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -35,25 +36,26 @@ input:
         type: file
         description: Sequence dictionary
         pattern: "*.dict"
+        ontologies: []
 output:
-  - interval_list:
-      - meta:
+  interval_list:
+    - - meta:
           type: file
           description: gatk interval list file
           pattern: "*.interval_list"
+          ontologies: []
       - "*.interval_list":
           type: file
           description: gatk interval list file
           pattern: "*.interval_list"
-      - _list:
-          type: file
-          description: gatk interval list file
-          pattern: "*.interval_list"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@kevinmenden"
   - "@ramprasadn"
diff --git a/modules/nf-core/gatk4/createsequencedictionary/main.nf b/modules/nf-core/gatk4/createsequencedictionary/main.nf
index 998622a06..872648a5e 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/main.nf
+++ b/modules/nf-core/gatk4/createsequencedictionary/main.nf
@@ -1,18 +1,18 @@
 process GATK4_CREATESEQUENCEDICTIONARY {
-    tag "$fasta"
+    tag "${fasta}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd'}"
 
     input:
     tuple val(meta), path(fasta)
 
     output:
-    tuple val(meta), path('*.dict')  , emit: dict
-    path "versions.yml"              , emit: versions
+    tuple val(meta), path('*.dict'), emit: dict
+    path "versions.yml",             emit: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -22,17 +22,18 @@ process GATK4_CREATESEQUENCEDICTIONARY {
 
     def avail_mem = 6144
     if (!task.memory) {
-        log.info '[GATK CreateSequenceDictionary] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK CreateSequenceDictionary] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         CreateSequenceDictionary \\
-        --REFERENCE $fasta \\
-        --URI $fasta \\
+        --REFERENCE ${fasta} \\
+        --URI ${fasta} \\
         --TMP_DIR . \\
-        $args
+        ${args}
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/gatk4/createsequencedictionary/meta.yml
index 7b5156bb3..72dced28c 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/meta.yml
+++ b/modules/nf-core/gatk4/createsequencedictionary/meta.yml
@@ -26,21 +26,26 @@ input:
         type: file
         description: Input fasta file
         pattern: "*.{fasta,fa}"
+        ontologies: []
 output:
-  - dict:
-      - meta:
+  dict:
+    - - meta:
           type: file
           description: gatk dictionary file
           pattern: "*.{dict}"
+          ontologies: []
       - "*.dict":
           type: file
           description: gatk dictionary file
           pattern: "*.{dict}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@maxulysse"
   - "@ramprasadn"
diff --git a/modules/nf-core/gatk4/markduplicates/main.nf b/modules/nf-core/gatk4/markduplicates/main.nf
index f4bd896bf..5fb161b4f 100644
--- a/modules/nf-core/gatk4/markduplicates/main.nf
+++ b/modules/nf-core/gatk4/markduplicates/main.nf
@@ -13,12 +13,12 @@ process GATK4_MARKDUPLICATES {
     path fasta_fai
 
     output:
-    tuple val(meta), path("*cram"), emit: cram, optional: true
-    tuple val(meta), path("*bam"), emit: bam, optional: true
-    tuple val(meta), path("*.crai"), emit: crai, optional: true
-    tuple val(meta), path("*.bai"), emit: bai, optional: true
+    tuple val(meta), path("*cram"),     emit: cram, optional: true
+    tuple val(meta), path("*bam"),      emit: bam,  optional: true
+    tuple val(meta), path("*.crai"),    emit: crai, optional: true
+    tuple val(meta), path("*.bai"),     emit: bai,  optional: true
     tuple val(meta), path("*.metrics"), emit: metrics
-    path "versions.yml", emit: versions
+    path "versions.yml",                emit: versions
 
     when:
     task.ext.when == null || task.ext.when
diff --git a/modules/nf-core/gatk4/markduplicates/meta.yml b/modules/nf-core/gatk4/markduplicates/meta.yml
index 4772c5f39..335c309a9 100644
--- a/modules/nf-core/gatk4/markduplicates/meta.yml
+++ b/modules/nf-core/gatk4/markduplicates/meta.yml
@@ -28,17 +28,20 @@ input:
         type: file
         description: Sorted BAM file
         pattern: "*.{bam}"
-  - - fasta:
-        type: file
-        description: Fasta file
-        pattern: "*.{fasta}"
-  - - fasta_fai:
-        type: file
-        description: Fasta index file
-        pattern: "*.{fai}"
+        ontologies: []
+  - fasta:
+      type: file
+      description: Fasta file
+      pattern: "*.{fasta}"
+      ontologies: []
+  - fasta_fai:
+      type: file
+      description: Fasta index file
+      pattern: "*.{fai}"
+      ontologies: []
 output:
-  - cram:
-      - meta:
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -47,8 +50,9 @@ output:
           type: file
           description: Marked duplicates CRAM file
           pattern: "*.{cram}"
-  - bam:
-      - meta:
+          ontologies: []
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -57,8 +61,9 @@ output:
           type: file
           description: Marked duplicates BAM file
           pattern: "*.{bam}"
-  - crai:
-      - meta:
+          ontologies: []
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -67,8 +72,9 @@ output:
           type: file
           description: CRAM index file
           pattern: "*.{cram.crai}"
-  - bai:
-      - meta:
+          ontologies: []
+  bai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -77,8 +83,9 @@ output:
           type: file
           description: BAM index file
           pattern: "*.{bam.bai}"
-  - metrics:
-      - meta:
+          ontologies: []
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -87,11 +94,14 @@ output:
           type: file
           description: Duplicate metrics file generated by GATK
           pattern: "*.{metrics.txt}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@ajodeh-juma"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gffread/meta.yml b/modules/nf-core/gffread/meta.yml
index bebe7f575..715efec8e 100644
--- a/modules/nf-core/gffread/meta.yml
+++ b/modules/nf-core/gffread/meta.yml
@@ -25,35 +25,39 @@ input:
         type: file
         description: A reference file in either the GFF3, GFF2 or GTF format.
         pattern: "*.{gff, gtf}"
-  - - fasta:
-        type: file
-        description: A multi-fasta file with the genomic sequences
-        pattern: "*.{fasta,fa,faa,fas,fsa}"
+        ontologies: []
+  - fasta:
+      type: file
+      description: A multi-fasta file with the genomic sequences
+      pattern: "*.{fasta,fa,faa,fas,fsa}"
+      ontologies: []
 output:
-  - gtf:
-      - meta:
+  gtf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing meta data
             e.g. [ id:'test' ]
       - "*.gtf":
           type: file
-          description: GTF file resulting from the conversion of the GFF input file if
-            '-T' argument is present
+          description: GTF file resulting from the conversion of the GFF input file
+            if '-T' argument is present
           pattern: "*.{gtf}"
-  - gffread_gff:
-      - meta:
+          ontologies: []
+  gffread_gff:
+    - - meta:
           type: map
           description: |
             Groovy Map containing meta data
             e.g. [ id:'test' ]
       - "*.gff3":
           type: file
-          description: GFF3 file resulting from the conversion of the GFF input file if
-            '-T' argument is absent
+          description: GFF3 file resulting from the conversion of the GFF input file
+            if '-T' argument is absent
           pattern: "*.gff3"
-  - gffread_fasta:
-      - meta:
+          ontologies: []
+  gffread_fasta:
+    - - meta:
           type: map
           description: |
             Groovy Map containing meta data
@@ -63,11 +67,14 @@ output:
           description: Fasta file produced when either of '-w', '-x', '-y' parameters
             is present
           pattern: "*.fasta"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@edmundmiller"
 maintainers:
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index 812fc4c5e..f89370483 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.29
+  - bioconda::multiqc=1.30
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 0ac3c3699..67460de9a 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/multiqc:1.29--pyhdfd78af_0' :
-        'biocontainers/multiqc:1.29--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.30--pyhdfd78af_1' :
+        'biocontainers/multiqc:1.30--pyhdfd78af_1' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index 88e90571c..0d3f288bd 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,c1fe644a37468f6dae548d98bc72c2c1"
+                "versions.yml:md5,e65ce731db2128b8e4dd43d6e880fc1c"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.3"
         },
-        "timestamp": "2025-05-22T11:50:41.182332996"
+        "timestamp": "2025-07-10T08:06:23.563041241"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,c1fe644a37468f6dae548d98bc72c2c1"
+                "versions.yml:md5,e65ce731db2128b8e4dd43d6e880fc1c"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.3"
         },
-        "timestamp": "2025-05-22T11:51:22.448739369"
+        "timestamp": "2025-07-10T08:06:48.96226832"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,c1fe644a37468f6dae548d98bc72c2c1"
+                "versions.yml:md5,e65ce731db2128b8e4dd43d6e880fc1c"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.3"
         },
-        "timestamp": "2025-05-22T11:51:06.198928424"
+        "timestamp": "2025-07-10T08:06:40.627008706"
     }
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/picard/collectinsertsizemetrics/meta.yml b/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
index 0947048dc..1c4572c58 100644
--- a/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
+++ b/modules/nf-core/picard/collectinsertsizemetrics/meta.yml
@@ -24,9 +24,10 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
 output:
-  - metrics:
-      - meta:
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -35,8 +36,9 @@ output:
           type: file
           description: Values used by Picard to generate the insert size histograms
           pattern: "*.txt"
-  - histogram:
-      - meta:
+          ontologies: []
+  histogram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -45,11 +47,14 @@ output:
           type: file
           description: Insert size histogram in PDF format
           pattern: "*.pdf"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@FerriolCalvet"
 maintainers:
diff --git a/modules/nf-core/picard/collectrnaseqmetrics/meta.yml b/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
index 15d146baa..b8da63e9f 100644
--- a/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
+++ b/modules/nf-core/picard/collectrnaseqmetrics/meta.yml
@@ -27,18 +27,22 @@ input:
         type: file
         description: BAM/SAM file
         pattern: "*.{bam,sam}"
-  - - ref_flat:
-        type: file
-        description: Genome ref_flat file
-  - - fasta:
-        type: file
-        description: Genome fasta file
-  - - rrna_intervals:
-        type: file
-        description: Interval file of ribosomal RNA regions
+        ontologies: []
+  - ref_flat:
+      type: file
+      description: Genome ref_flat file
+      ontologies: []
+  - fasta:
+      type: file
+      description: Genome fasta file
+      ontologies: []
+  - rrna_intervals:
+      type: file
+      description: Interval file of ribosomal RNA regions
+      ontologies: []
 output:
-  - metrics:
-      - meta:
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -47,21 +51,25 @@ output:
           type: file
           description: RNA alignment metrics files generated by picard
           pattern: "*.rna_metrics"
-  - pdf:
-      - meta:
+          ontologies: []
+  pdf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.pdf":
           type: file
-          description: Plot normalized position vs. coverage in a pdf file generated by
-            picard
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          description: Plot normalized position vs. coverage in a pdf file generated
+            by picard
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@anoronh4"
 maintainers:
diff --git a/modules/nf-core/picard/collectwgsmetrics/meta.yml b/modules/nf-core/picard/collectwgsmetrics/meta.yml
index bb7480809..a27e0a88c 100644
--- a/modules/nf-core/picard/collectwgsmetrics/meta.yml
+++ b/modules/nf-core/picard/collectwgsmetrics/meta.yml
@@ -26,10 +26,12 @@ input:
         type: file
         description: Aligned reads file
         pattern: "*.{bam, cram}"
+        ontologies: []
     - bai:
         type: file
         description: (Optional) Aligned reads file index
         pattern: "*.{bai,crai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -39,6 +41,7 @@ input:
         type: file
         description: Genome fasta file
         pattern: "*.{fa,fasta,fna}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -48,13 +51,15 @@ input:
         type: file
         description: Genome fasta file index
         pattern: "*.{fai}"
-  - - intervallist:
-        type: file
-        description: Picard Interval List. Defines which contigs to include. Can be
-          generated from a BED file with GATK BedToIntervalList.
+        ontologies: []
+  - intervallist:
+      type: file
+      description: Picard Interval List. Defines which contigs to include. Can be generated
+        from a BED file with GATK BedToIntervalList.
+      ontologies: []
 output:
-  - metrics:
-      - meta:
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -63,11 +68,14 @@ output:
           type: file
           description: Alignment metrics files generated by picard
           pattern: "*_{metrics}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@drpatelh"
   - "@flowuenne"
diff --git a/modules/nf-core/salmon/index/meta.yml b/modules/nf-core/salmon/index/meta.yml
index 48486a2bb..4e0367b4a 100644
--- a/modules/nf-core/salmon/index/meta.yml
+++ b/modules/nf-core/salmon/index/meta.yml
@@ -15,23 +15,27 @@ tools:
       licence: ["GPL-3.0-or-later"]
       identifier: biotools:salmon
 input:
-  - - genome_fasta:
-        type: file
-        description: Fasta file of the reference genome
-  - - transcript_fasta:
-        type: file
-        description: Fasta file of the reference transcriptome
+  - genome_fasta:
+      type: file
+      description: Fasta file of the reference genome
+      ontologies: []
+  - transcript_fasta:
+      type: file
+      description: Fasta file of the reference transcriptome
+      ontologies: []
 output:
-  - index:
-      - salmon:
-          type: directory
-          description: Folder containing the star index files
-          pattern: "salmon"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+  index:
+    - salmon:
+        type: directory
+        description: Folder containing the star index files
+        pattern: "salmon"
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/salmon/quant/meta.yml b/modules/nf-core/salmon/quant/meta.yml
index 4cacde0f0..40e8eb965 100644
--- a/modules/nf-core/salmon/quant/meta.yml
+++ b/modules/nf-core/salmon/quant/meta.yml
@@ -26,25 +26,28 @@ input:
           List of input FastQ files for single-end or paired-end data.
           Multiple single-end fastqs or pairs of paired-end fastqs are
           handled.
-  - - index:
-        type: directory
-        description: Folder containing the star index files
-  - - gtf:
-        type: file
-        description: GTF of the reference transcriptome
-  - - transcript_fasta:
-        type: file
-        description: Fasta file of the reference transcriptome
-  - - alignment_mode:
-        type: boolean
-        description: whether to run salmon in alignment mode
-  - - lib_type:
-        type: string
-        description: |
-          Override library type inferred based on strandedness defined in meta object
+        ontologies: []
+  - index:
+      type: directory
+      description: Folder containing the star index files
+  - gtf:
+      type: file
+      description: GTF of the reference transcriptome
+      ontologies: []
+  - transcript_fasta:
+      type: file
+      description: Fasta file of the reference transcriptome
+      ontologies: []
+  - alignment_mode:
+      type: boolean
+      description: whether to run salmon in alignment mode
+  - lib_type:
+      type: string
+      description: |
+        Override library type inferred based on strandedness defined in meta object
 output:
-  - results:
-      - meta:
+  results:
+    - - meta:
           type: directory
           description: Folder containing the quantification results for a specific sample
           pattern: "${prefix}"
@@ -52,30 +55,35 @@ output:
           type: directory
           description: Folder containing the quantification results for a specific sample
           pattern: "${prefix}"
-  - json_info:
-      - meta:
-          type: file
-          description: File containing meta information from Salmon quant
-          pattern: "*info.json"
+  json_info:
+    - - meta:
+          type: directory
+          description: Folder containing the quantification results for a specific sample
+          pattern: "${prefix}"
       - "*info.json":
           type: file
           description: File containing meta information from Salmon quant
           pattern: "*info.json"
-  - lib_format_counts:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
+          ontologies:
+            - edam: http://edamontology.org/format_3464 # JSON
+  lib_format_counts:
+    - - meta:
+          type: directory
+          description: Folder containing the quantification results for a specific sample
+          pattern: "${prefix}"
       - "*lib_format_counts.json":
           type: file
           description: File containing the library format counts
           pattern: "*lib_format_counts.json"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3464 # JSON
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/samtools/convert/meta.yml b/modules/nf-core/samtools/convert/meta.yml
index d5bfa161b..519812ab5 100644
--- a/modules/nf-core/samtools/convert/meta.yml
+++ b/modules/nf-core/samtools/convert/meta.yml
@@ -26,10 +26,12 @@ input:
         type: file
         description: BAM/CRAM file
         pattern: "*.{bam,cram}"
+        ontologies: []
     - index:
         type: file
         description: BAM/CRAM index file
         pattern: "*.{bai,crai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -39,6 +41,7 @@ input:
         type: file
         description: Reference file to create the CRAM file
         pattern: "*.{fasta,fa}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -48,9 +51,10 @@ input:
         type: file
         description: Reference index file to create the CRAM file
         pattern: "*.{fai}"
+        ontologies: []
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -59,8 +63,9 @@ output:
           type: file
           description: filtered/converted BAM file
           pattern: "*{.bam}"
-  - cram:
-      - meta:
+          ontologies: []
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -69,8 +74,9 @@ output:
           type: file
           description: filtered/converted CRAM file
           pattern: "*{cram}"
-  - bai:
-      - meta:
+          ontologies: []
+  bai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -79,8 +85,9 @@ output:
           type: file
           description: filtered/converted BAM index
           pattern: "*{.bai}"
-  - crai:
-      - meta:
+          ontologies: []
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -89,11 +96,14 @@ output:
           type: file
           description: filtered/converted CRAM index
           pattern: "*{.crai}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@FriederikeHanssen"
   - "@maxulysse"
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
index 256a330a1..b7a2e0c1a 100644
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -26,6 +26,7 @@ input:
         type: file
         description: FASTA file
         pattern: "*.{fa,fasta}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -35,13 +36,14 @@ input:
         type: file
         description: FASTA index file
         pattern: "*.{fai}"
-  - - get_sizes:
-        type: boolean
-        description: use cut to get the sizes of the index (true) or not (false)
+        ontologies: []
+  - get_sizes:
+      type: boolean
+      description: use cut to get the sizes of the index (true) or not (false)
 
 output:
-  - fa:
-      - meta:
+  fa:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -50,28 +52,31 @@ output:
           type: file
           description: FASTA file
           pattern: "*.{fa}"
-  - fai:
-      - meta:
+          ontologies: []
+  sizes:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.fai":
+      - "*.sizes":
           type: file
-          description: FASTA index file
-          pattern: "*.{fai}"
-  - sizes:
-      - meta:
+          description: File containing chromosome lengths
+          pattern: "*.{sizes}"
+          ontologies: []
+  fai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.sizes":
+      - "*.fai":
           type: file
-          description: File containing chromosome lengths
-          pattern: "*.{sizes}"
-  - gzi:
-      - meta:
+          description: FASTA index file
+          pattern: "*.{fai}"
+          ontologies: []
+  gzi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -80,11 +85,14 @@ output:
           type: file
           description: Optional gzip index file for compressed inputs
           pattern: "*.gzi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@drpatelh"
   - "@ewels"
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index 311756102..7019a72e4 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -24,7 +24,7 @@ process SAMTOOLS_INDEX {
     """
     samtools \\
         index \\
-        -@ ${task.cpus-1} \\
+        -@ ${task.cpus} \\
         $args \\
         $input
 
diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml
index db8df0d50..1bed6bca6 100644
--- a/modules/nf-core/samtools/index/meta.yml
+++ b/modules/nf-core/samtools/index/meta.yml
@@ -25,9 +25,10 @@ input:
     - input:
         type: file
         description: input file
+        ontologies: []
 output:
-  - bai:
-      - meta:
+  bai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -36,8 +37,9 @@ output:
           type: file
           description: BAM/CRAM/SAM index file
           pattern: "*.{bai,crai,sai}"
-  - csi:
-      - meta:
+          ontologies: []
+  csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -46,8 +48,9 @@ output:
           type: file
           description: CSI index file
           pattern: "*.{csi}"
-  - crai:
-      - meta:
+          ontologies: []
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -56,11 +59,14 @@ output:
           type: file
           description: BAM/CRAM/SAM index file
           pattern: "*.{bai,crai,sai}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@drpatelh"
   - "@ewels"
diff --git a/modules/nf-core/star/align/meta.yml b/modules/nf-core/star/align/meta.yml
index 5cfe763e3..1ee469052 100644
--- a/modules/nf-core/star/align/meta.yml
+++ b/modules/nf-core/star/align/meta.yml
@@ -26,6 +26,7 @@ input:
         description: |
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively.
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -44,18 +45,19 @@ input:
         type: file
         description: Annotation GTF file
         pattern: "*.{gtf}"
-  - - star_ignore_sjdbgtf:
-        type: boolean
-        description: Ignore annotation GTF file
-  - - seq_platform:
-        type: string
-        description: Sequencing platform
-  - - seq_center:
-        type: string
-        description: Sequencing center
+        ontologies: []
+  - star_ignore_sjdbgtf:
+      type: boolean
+      description: Ignore annotation GTF file
+  - seq_platform:
+      type: string
+      description: Sequencing platform
+  - seq_center:
+      type: string
+      description: Sequencing center
 output:
-  - log_final:
-      - meta:
+  log_final:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -64,8 +66,9 @@ output:
           type: file
           description: STAR final log file
           pattern: "*Log.final.out"
-  - log_out:
-      - meta:
+          ontologies: []
+  log_out:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -74,8 +77,9 @@ output:
           type: file
           description: STAR lot out file
           pattern: "*Log.out"
-  - log_progress:
-      - meta:
+          ontologies: []
+  log_progress:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -84,13 +88,16 @@ output:
           type: file
           description: STAR log progress file
           pattern: "*Log.progress.out"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
-  - bam:
-      - meta:
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -99,8 +106,9 @@ output:
           type: file
           description: Output BAM file containing read alignments
           pattern: "*.{bam}"
-  - bam_sorted:
-      - meta:
+          ontologies: []
+  bam_sorted:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -109,8 +117,9 @@ output:
           type: file
           description: Sorted BAM file of read alignments (optional)
           pattern: "*sortedByCoord.out.bam"
-  - bam_sorted_aligned:
-      - meta:
+          ontologies: []
+  bam_sorted_aligned:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -119,8 +128,9 @@ output:
           type: file
           description: Sorted BAM file of read alignments (optional)
           pattern: "*.Aligned.sortedByCoord.out.bam"
-  - bam_transcript:
-      - meta:
+          ontologies: []
+  bam_transcript:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -129,8 +139,9 @@ output:
           type: file
           description: Output BAM file of transcriptome alignment (optional)
           pattern: "*toTranscriptome.out.bam"
-  - bam_unsorted:
-      - meta:
+          ontologies: []
+  bam_unsorted:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -139,8 +150,9 @@ output:
           type: file
           description: Unsorted BAM file of read alignments (optional)
           pattern: "*Aligned.unsort.out.bam"
-  - fastq:
-      - meta:
+          ontologies: []
+  fastq:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -149,8 +161,10 @@ output:
           type: file
           description: Unmapped FastQ files (optional)
           pattern: "*fastq.gz"
-  - tab:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  tab:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -159,8 +173,10 @@ output:
           type: file
           description: STAR output tab file(s) (optional)
           pattern: "*.tab"
-  - spl_junc_tab:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+  spl_junc_tab:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -169,8 +185,10 @@ output:
           type: file
           description: STAR output splice junction tab file
           pattern: "*.SJ.out.tab"
-  - read_per_gene_tab:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+  read_per_gene_tab:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -179,8 +197,10 @@ output:
           type: file
           description: STAR output read per gene tab file
           pattern: "*.ReadsPerGene.out.tab"
-  - junction:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+  junction:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -189,19 +209,20 @@ output:
           type: file
           description: STAR chimeric junction output file (optional)
           pattern: "*.out.junction"
-  - sam:
-      - meta:
+          ontologies: []
+  sam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*.out.sam"
       - "*.out.sam":
           type: file
           description: STAR output SAM file(s) (optional)
           pattern: "*.out.sam"
-  - wig:
-      - meta:
+          ontologies: []
+  wig:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -210,8 +231,9 @@ output:
           type: file
           description: STAR output wiggle format file(s) (optional)
           pattern: "*.wig"
-  - bedgraph:
-      - meta:
+          ontologies: []
+  bedgraph:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -220,6 +242,7 @@ output:
           type: file
           description: STAR output bedGraph format file(s) (optional)
           pattern: "*.bg"
+          ontologies: []
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/star/genomegenerate/meta.yml b/modules/nf-core/star/genomegenerate/meta.yml
index 33c1f65f3..39ed3cbde 100644
--- a/modules/nf-core/star/genomegenerate/meta.yml
+++ b/modules/nf-core/star/genomegenerate/meta.yml
@@ -24,6 +24,7 @@ input:
     - fasta:
         type: file
         description: Fasta file of the reference genome
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -32,9 +33,10 @@ input:
     - gtf:
         type: file
         description: GTF file of the reference genome
+        ontologies: []
 output:
-  - index:
-      - meta:
+  index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -43,11 +45,13 @@ output:
           type: directory
           description: Folder containing the star index files
           pattern: "star"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@kevinmenden"
   - "@drpatelh"
diff --git a/modules/nf-core/starfusion/build/tests/main.nf.test b/modules/nf-core/starfusion/build/tests/main.nf.test
index ae18299e2..2fa9f044c 100644
--- a/modules/nf-core/starfusion/build/tests/main.nf.test
+++ b/modules/nf-core/starfusion/build/tests/main.nf.test
@@ -23,13 +23,13 @@ nextflow_process {
 
                 input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                 input[3] = "homo_sapiens"
-                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[4] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/Pfam-A.hmm.gz')
                 input[5] = [
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3f'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3i'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3m'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3p')
                 ]
                 input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                 """
@@ -125,22 +125,22 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'minigenome_fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
                 ]
                 input[1] = [
                     [ id:'minigenome_gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
                 ]
 
-                input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                 input[3] = "homo_sapiens"
-                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[4] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/Pfam-A.hmm.gz')
                 input[5] = [
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3p"
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3f'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3i'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3m'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3p')
                 ]
                 input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                 """
@@ -160,21 +160,21 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id:'minigenome_fasta' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.fa")
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.fa')
                 ]
                 input[1] = [
                     [ id:'minigenome_gtf' ],
-                    file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/minigenome.gtf")
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/minigenome.gtf')
                 ]
 
-                input[2] = file("https://github.com/STAR-Fusion/STAR-Fusion-Tutorial/raw/master/CTAT_HumanFusionLib.mini.dat.gz")
+                input[2] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/CTAT_HumanFusionLib.mini.dat.gz')
                 input[3] = "homo_sapiens"
-                input[4] = "http://ftp.ebi.ac.uk/pub/databases/Pfam/releases/Pfam37.4/Pfam-A.hmm.gz"
+                input[4] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/Pfam-A.hmm.gz')
                 input[5] = [
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3f",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3i",
-                    "https://www.dfam.org/releases/Dfam_3.8/infrastructure/dfamscan/homo_sapiens_dfam.hmm.h3m"
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3f'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3i'),
+                    file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/test_starfusion_dfam.hmm.h3m')
                 ]
                 input[6] = "https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/AnnotFilterRule.pm"
                 """
diff --git a/modules/nf-core/stringtie/merge/meta.yml b/modules/nf-core/stringtie/merge/meta.yml
index cf6902b32..3201746c2 100644
--- a/modules/nf-core/stringtie/merge/meta.yml
+++ b/modules/nf-core/stringtie/merge/meta.yml
@@ -13,27 +13,32 @@ tools:
       licence: ["MIT"]
       identifier: biotools:stringtie
 input:
-  - - stringtie_gtf:
+  - stringtie_gtf:
+      type: file
+      description: |
+        Stringtie transcript gtf output(s).
+      pattern: "*.gtf"
+      ontologies: []
+  - annotation_gtf:
+      type: file
+      description: |
+        Annotation gtf file (optional).
+      pattern: "*.gtf"
+      ontologies: []
+output:
+  gtf:
+    - stringtie.merged.gtf:
         type: file
-        description: |
-          Stringtie transcript gtf output(s).
-        pattern: "*.gtf"
-  - - annotation_gtf:
+        description: Merged gtf file
+        pattern: "stringtie.merged.gtf"
+        ontologies: []
+  versions:
+    - versions.yml:
         type: file
-        description: |
-          Annotation gtf file (optional).
-        pattern: "*.gtf"
-output:
-  - gtf:
-      - stringtie.merged.gtf:
-          type: file
-          description: Merged gtf file
-          pattern: "stringtie.merged.gtf"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@yuukiiwa"
 maintainers:
diff --git a/modules/nf-core/stringtie/stringtie/meta.yml b/modules/nf-core/stringtie/stringtie/meta.yml
index e55b2abfc..9cdd14004 100644
--- a/modules/nf-core/stringtie/stringtie/meta.yml
+++ b/modules/nf-core/stringtie/stringtie/meta.yml
@@ -23,13 +23,15 @@ input:
         type: file
         description: |
           Stringtie transcript gtf output(s).
-  - - annotation_gtf:
-        type: file
-        description: |
-          Annotation gtf file (optional).
+        ontologies: []
+  - annotation_gtf:
+      type: file
+      description: |
+        Annotation gtf file (optional).
+      ontologies: []
 output:
-  - transcript_gtf:
-      - meta:
+  transcript_gtf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -38,8 +40,9 @@ output:
           type: file
           description: transcript gtf
           pattern: "*.{transcripts.gtf}"
-  - abundance:
-      - meta:
+          ontologies: []
+  abundance:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -48,8 +51,9 @@ output:
           type: file
           description: abundance
           pattern: "*.{abundance.txt}"
-  - coverage_gtf:
-      - meta:
+          ontologies: []
+  coverage_gtf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -58,8 +62,9 @@ output:
           type: file
           description: coverage gtf
           pattern: "*.{coverage.gtf}"
-  - ballgown:
-      - meta:
+          ontologies: []
+  ballgown:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -68,11 +73,14 @@ output:
           type: file
           description: for running ballgown
           pattern: "*.{ballgown}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@drpatelh"
 maintainers:
diff --git a/modules/nf-core/ucsc/gtftogenepred/meta.yml b/modules/nf-core/ucsc/gtftogenepred/meta.yml
index cf04154d4..5939538df 100644
--- a/modules/nf-core/ucsc/gtftogenepred/meta.yml
+++ b/modules/nf-core/ucsc/gtftogenepred/meta.yml
@@ -22,9 +22,10 @@ input:
         type: file
         description: GTF file
         pattern: "*.{gtf}"
+        ontologies: []
 output:
-  - genepred:
-      - meta:
+  genepred:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -33,8 +34,9 @@ output:
           type: file
           description: genepred file
           pattern: "*.{genepred}"
-  - refflat:
-      - meta:
+          ontologies: []
+  refflat:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -43,11 +45,14 @@ output:
           type: file
           description: refflat file
           pattern: "*.{refflat}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions:
+    - versions.yml:
+        type: file
+        description: File containing software versions
+        pattern: "versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
 authors:
   - "@BarryDigby"
   - "@anoronh4"
diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index 40257fa61..7324d8323 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -31,7 +31,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
         if (whitelist)  {
             ch_whitelist = ch_fusion_list.combine(Channel.value(file(whitelist, checkIfExists:true)))
-                            .map { meta, fusions, whitelist -> [ meta, [fusions, whitelist] ] }
+                            .map { meta, fusions, whitelist_file -> [ meta, [fusions, whitelist_file] ] }
 
             CAT_CAT(ch_whitelist) // fusioninspector takes care of possible duplicates
             ch_versions = ch_versions.mix(CAT_CAT.out.versions)

From 920313ac21a1db80e1d269644d25471a0776ead7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 3 Sep 2025 13:51:37 +0200
Subject: [PATCH 843/887] update subwfs, solve linting issues

---
 README.md                                     |  2 +-
 modules.json                                  |  6 +--
 nextflow.config                               | 37 +++++++++++++++----
 nextflow_schema.json                          | 11 ++++--
 ro-crate-metadata.json                        |  2 +-
 subworkflows/local/build_references/main.nf   |  1 -
 .../utils_nextflow_pipeline/tests/tags.yml    |  2 -
 .../utils_nfcore_pipeline/tests/tags.yml      |  2 -
 .../tests/nextflow.config                     |  2 +-
 9 files changed, 44 insertions(+), 21 deletions(-)
 delete mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
 delete mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml

diff --git a/README.md b/README.md
index 6da8f3b1a..6f1a9fd19 100644
--- a/README.md
+++ b/README.md
@@ -6,7 +6,7 @@
 </h1>
 
 [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)
-[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
+[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.2565517-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.2565517)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
 [![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
diff --git a/modules.json b/modules.json
index fb18b3dc9..9505147f7 100644
--- a/modules.json
+++ b/modules.json
@@ -238,17 +238,17 @@
                     },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
+                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
+                        "git_sha": "dcd088f483cede0c3df4034d405126f05a764cc7",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/nextflow.config b/nextflow.config
index ef54ad4e6..bf0f24ca1 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -301,23 +301,46 @@ dag {
 manifest {
     name            = 'nf-core/rnafusion'
     contributors    = [
-        // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0
         [
             name: 'Martin Proks',
             affiliation: '',
             email: '',
-            github: '',
-            contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor')
+            github: '@matq007',
+            contribution: ['author'],
             orcid: ''
         ],
         [
-            name: ' Annick Renevey',
-            affiliation: '',
+            name: 'Annick Renevey',
+            affiliation: 'SciLifeLab, Karolinska Institutet',
+            email: 'annick.renevey@scilifelab.se',
+            github: '@rannick',
+            contribution: ['author', 'maintainer'],
+            orcid: '0000-0001-7411-6063'
+        ],
+        [
+            name: 'Maxime U Garcia',
+            affiliation: 'Seqera',
+            email: 'maxime.garcia@seqera.io',
+            github: '@maxulysse',
+            contribution: ['author'],
+            orcid: '0000-0003-2827-9261'
+        ],
+        [
+            name: 'Anabella Trigila',
+            affiliation: 'ZS Associates',
             email: '',
-            github: '',
-            contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor')
+            github: '@atrigila',
+            contribution: ['contributor', 'maintainer'],
             orcid: ''
         ],
+        [
+            name: 'Nicolas Vannieuwkerke',
+            affiliation: 'Center for Medical Genetics Ghent',
+            email: '',
+            github: '@nvnieuwk',
+            contribution: ['contributor', 'maintainer'],
+            orcid: ''
+        ]
     ]
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = """Nextflow rnafusion analysis pipeline, part of the nf-core community."""
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 5b15693b1..478c27b40 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -39,6 +39,7 @@
                 "input": {
                     "type": "string",
                     "format": "file-path",
+                    "mimetype": "text/csv",
                     "schema": "assets/schema_input.json",
                     "exists": true,
                     "pattern": "^\\S+\\.(csv|yaml|yml|json)$",
@@ -335,16 +336,20 @@
                 },
                 "save_trimmed_fail": {
                     "type": "boolean",
-                    "fa_icon": "far fa-times-circle"
+                    "fa_icon": "far fa-times-circle",
+                    "description": "FASTP: Specify true to save files that failed to pass trimming thresholds"
                 },
                 "min_trimmed_reads": {
                     "type": "integer",
                     "default": 1,
-                    "fa_icon": "fas fa-search-minus"
+                    "fa_icon": "fas fa-search-minus",
+                    "description": "FASTP: Inputs with fewer than this reads will be filtered out of the \"reads\" output channel"
+
                 },
                 "save_merged": {
                     "type": "boolean",
-                    "fa_icon": "fas fa-download"
+                    "fa_icon": "fas fa-download",
+                    "description": "FASTP: Specify true to save merged reads"
                 }
             }
         },
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 3925e8228..bcd77eb1e 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2025-07-08T11:39:11+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source. See the [References](#references) section for more information.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n   7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)\n   7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n7. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n8. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n9. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - Summarize information into a VCF file\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.2565517-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.2565517)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source. See the [References](#references) section for more information.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n   7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)\n   7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n7. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n8. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n9. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - Summarize information into a VCF file\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
diff --git a/subworkflows/local/build_references/main.nf b/subworkflows/local/build_references/main.nf
index 7ac3285a2..78d413709 100644
--- a/subworkflows/local/build_references/main.nf
+++ b/subworkflows/local/build_references/main.nf
@@ -24,7 +24,6 @@ include { SALMON_INDEX }                    from '../../../modules/nf-core/salmo
 include { FUSIONREPORT_DOWNLOAD }           from '../../../modules/nf-core/fusionreport/download/main'
 include { STARFUSION_BUILD }                from '../../../modules/nf-core/starfusion/build/main'
 include { GFFREAD }                         from '../../../modules/nf-core/gffread/main'
-include { getFileSuffix } from '../../../modules/nf-core/cat/cat/main.nf'
 
 /*
 ========================================================================================
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
deleted file mode 100644
index f84761125..000000000
--- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-subworkflows/utils_nextflow_pipeline:
-  - subworkflows/nf-core/utils_nextflow_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
deleted file mode 100644
index ac8523c9a..000000000
--- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
+++ /dev/null
@@ -1,2 +0,0 @@
-subworkflows/utils_nfcore_pipeline:
-  - subworkflows/nf-core/utils_nfcore_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
index 09ef842ae..443e828cf 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
@@ -5,4 +5,4 @@ plugins {
 validation {
     parametersSchema = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
     monochromeLogs = true
-}
\ No newline at end of file
+}

From 5276450cbe785eb57d21bd12511a18ef8bc819a6 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 3 Sep 2025 13:53:12 +0200
Subject: [PATCH 844/887] prettier

---
 docs/output.md       | 1 +
 nextflow_schema.json | 1 -
 2 files changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index 9937f7fb4..e2a5c4391 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -283,6 +283,7 @@ The weights for databases are as follows:
 The Fusion Indication Index FII is calculated using two components:
 
 1. Tool Detection (50% of total FII)
+
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 478c27b40..b6ce64d69 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -344,7 +344,6 @@
                     "default": 1,
                     "fa_icon": "fas fa-search-minus",
                     "description": "FASTP: Inputs with fewer than this reads will be filtered out of the \"reads\" output channel"
-
                 },
                 "save_merged": {
                     "type": "boolean",

From 99314904b2ca0be0efeb7bf30ff0e85105c9d922 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 3 Sep 2025 15:22:09 +0200
Subject: [PATCH 845/887] update snaps

---
 tests/test_stub.nf.test.snap     | 55 +++++++++++++-------------------
 tests/test_stub_bam.nf.test.snap | 50 ++++++++++++-----------------
 2 files changed, 42 insertions(+), 63 deletions(-)

diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index aa25d1c31..344aaf4c6 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -82,8 +82,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -107,6 +105,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -237,7 +236,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:22:03.749618742"
+        "timestamp": "2025-09-03T14:26:01.049466"
     },
     "stub test stringtie": {
         "content": [
@@ -320,8 +319,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -345,6 +342,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -402,7 +400,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:22:39.560551811"
+        "timestamp": "2025-09-03T14:27:50.733492"
     },
     "stub test arriba": {
         "content": [
@@ -485,8 +483,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -510,6 +506,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -560,7 +557,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:19:38.026180355"
+        "timestamp": "2025-09-03T14:20:11.549426"
     },
     "stub test fusioninspector": {
         "content": [
@@ -678,8 +675,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -709,6 +704,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -838,7 +834,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:45:07.811039"
+        "timestamp": "2025-09-03T14:34:09.933192"
     },
     "stub test salmon": {
         "content": [
@@ -889,8 +885,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -906,6 +900,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -934,7 +929,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:43:03.790312"
+        "timestamp": "2025-09-03T14:32:07.557489"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -1034,8 +1029,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1059,6 +1052,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -1190,7 +1184,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T15:57:11.157812"
+        "timestamp": "2025-09-03T14:21:57.973003"
     },
     "stub test fusionreport": {
         "content": [
@@ -1302,8 +1296,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1333,6 +1325,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -1463,7 +1456,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:40:47.748229"
+        "timestamp": "2025-09-03T14:29:47.966689"
     },
     "stub test fastp": {
         "content": [
@@ -1520,8 +1513,6 @@
                 "fastqc_for_fastp",
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1536,6 +1527,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1544,7 +1536,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:41:54.637163"
+        "timestamp": "2025-09-03T14:30:56.64966"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1593,8 +1585,6 @@
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1609,6 +1599,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1617,7 +1608,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T15:58:18.715882"
+        "timestamp": "2025-09-03T14:23:05.549559"
     },
     "stub test fusioncatcher trim": {
         "content": [
@@ -1675,8 +1666,6 @@
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1691,6 +1680,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1699,7 +1689,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T15:59:24.345391"
+        "timestamp": "2025-09-03T14:24:14.169033"
     },
     "stub test all": {
         "content": [
@@ -1875,8 +1865,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1907,6 +1895,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -2072,6 +2061,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T15:53:38.903818"
+        "timestamp": "2025-09-03T14:18:24.876773"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index 8021399a8..90240c794 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -62,8 +62,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -87,6 +85,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -192,7 +191,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:58:08.911034"
+        "timestamp": "2025-09-03T15:03:29.812853"
     },
     "stub test stringtie": {
         "content": [
@@ -255,8 +254,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -280,6 +277,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -315,7 +313,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:59:44.881636"
+        "timestamp": "2025-09-03T15:05:06.630556"
     },
     "stub test arriba": {
         "content": [
@@ -378,8 +376,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -403,6 +399,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -428,7 +425,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:53:13.922071"
+        "timestamp": "2025-09-03T14:58:37.480648"
     },
     "stub test fusioninspector": {
         "content": [
@@ -526,8 +523,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -557,6 +552,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -664,7 +660,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T17:06:31.98408"
+        "timestamp": "2025-09-03T15:11:58.199402"
     },
     "stub test salmon": {
         "content": [
@@ -725,8 +721,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -751,6 +745,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -779,7 +774,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T17:04:40.402707"
+        "timestamp": "2025-09-03T15:10:03.376978"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -859,8 +854,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -884,6 +877,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -993,7 +987,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:54:50.384883"
+        "timestamp": "2025-09-03T15:00:17.678361"
     },
     "stub test fusionreport": {
         "content": [
@@ -1085,8 +1079,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1116,6 +1108,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -1221,7 +1214,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T17:01:37.589889"
+        "timestamp": "2025-09-03T15:06:51.996134"
     },
     "stub test fastp": {
         "content": [
@@ -1288,8 +1281,6 @@
                 "fastqc_for_fastp",
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1313,6 +1304,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1321,7 +1313,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T17:03:09.838745"
+        "timestamp": "2025-09-03T15:08:28.85535"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1380,8 +1372,6 @@
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1405,6 +1395,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1413,7 +1404,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:56:26.925856"
+        "timestamp": "2025-09-03T15:01:52.523177"
     },
     "stub test all": {
         "content": [
@@ -1569,8 +1560,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1601,6 +1590,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -1744,6 +1734,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T16:51:37.37908"
+        "timestamp": "2025-09-03T14:56:57.734145"
     }
 }
\ No newline at end of file

From 0fdf38a2003a1757de92981ea376c21de96690ce Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 4 Sep 2025 21:13:40 +0200
Subject: [PATCH 846/887] update snap

---
 .../tests/main.nf.test                        |  2 +-
 tests/test_stub.nf.test.snap                  | 55 ++++++++-----------
 tests/test_stub_bam.nf.test.snap              | 50 +++++++----------
 3 files changed, 43 insertions(+), 64 deletions(-)

diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
index e02352e94..746763ee7 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
@@ -110,7 +110,7 @@ nextflow_workflow {
                 input[12] = false
                 input[13] = true
                 input[14] = 1
-                input[15] = params.whitelist
+                input[15] = []
                 """
             }
         }
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index 5a3a1bc32..e54f807af 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -82,8 +82,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -107,6 +105,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -237,7 +236,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:22:03.749618742"
+        "timestamp": "2025-09-04T17:13:34.296716"
     },
     "stub test stringtie": {
         "content": [
@@ -320,8 +319,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -345,6 +342,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -402,7 +400,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:22:39.560551811"
+        "timestamp": "2025-09-04T17:15:18.001786"
     },
     "stub test arriba": {
         "content": [
@@ -485,8 +483,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -510,6 +506,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -560,7 +557,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:19:38.026180355"
+        "timestamp": "2025-09-04T17:07:39.086364"
     },
     "stub test fusioninspector": {
         "content": [
@@ -691,8 +688,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -722,6 +717,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -853,7 +849,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:25:11.604461904"
+        "timestamp": "2025-09-04T17:21:42.479016"
     },
     "stub test salmon": {
         "content": [
@@ -904,8 +900,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -921,6 +915,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -949,7 +944,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:24:28.067668122"
+        "timestamp": "2025-09-04T17:19:36.873298"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -1049,8 +1044,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1074,6 +1067,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -1205,7 +1199,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:20:13.319176919"
+        "timestamp": "2025-09-04T17:09:29.991365"
     },
     "stub test fusionreport": {
         "content": [
@@ -1317,8 +1311,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1348,6 +1340,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -1478,7 +1471,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:23:24.792491035"
+        "timestamp": "2025-09-04T17:17:14.759248"
     },
     "stub test fastp": {
         "content": [
@@ -1535,8 +1528,6 @@
                 "fastqc_for_fastp",
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1551,6 +1542,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1559,7 +1551,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:23:56.858216733"
+        "timestamp": "2025-09-04T17:18:27.651568"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1608,8 +1600,6 @@
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1624,6 +1614,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1632,7 +1623,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:20:49.030393254"
+        "timestamp": "2025-09-04T17:10:40.658609"
     },
     "stub test fusioncatcher trim": {
         "content": [
@@ -1690,8 +1681,6 @@
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1706,6 +1695,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1714,7 +1704,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:21:15.319414382"
+        "timestamp": "2025-09-04T17:11:47.659941"
     },
     "stub test all": {
         "content": [
@@ -1903,8 +1893,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1935,6 +1923,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -2102,6 +2091,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:19:03.489568486"
+        "timestamp": "2025-09-04T17:05:39.966652"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index af94c528c..5efbe384f 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -62,8 +62,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -87,6 +85,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -192,7 +191,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:29:13.77559568"
+        "timestamp": "2025-09-04T17:45:42.13657"
     },
     "stub test stringtie": {
         "content": [
@@ -255,8 +254,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -280,6 +277,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -315,7 +313,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:29:53.58622226"
+        "timestamp": "2025-09-04T17:47:52.517978"
     },
     "stub test arriba": {
         "content": [
@@ -378,8 +376,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -403,6 +399,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -428,7 +425,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:26:49.642086755"
+        "timestamp": "2025-09-04T17:40:41.686721"
     },
     "stub test fusioninspector": {
         "content": [
@@ -539,8 +536,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -570,6 +565,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -679,7 +675,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:32:46.154112416"
+        "timestamp": "2025-09-04T17:56:24.707034"
     },
     "stub test salmon": {
         "content": [
@@ -740,8 +736,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -766,6 +760,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -794,7 +789,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:31:56.747683511"
+        "timestamp": "2025-09-04T17:54:08.811545"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -874,8 +869,6 @@
                 "fastqc",
                 "fastqc/test.html",
                 "fastqc/test.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -899,6 +892,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -1008,7 +1002,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:27:33.646044212"
+        "timestamp": "2025-09-04T17:42:17.062793"
     },
     "stub test fusionreport": {
         "content": [
@@ -1100,8 +1094,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1131,6 +1123,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/star",
@@ -1236,7 +1229,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:30:34.704355039"
+        "timestamp": "2025-09-04T17:50:22.553497"
     },
     "stub test fastp": {
         "content": [
@@ -1303,8 +1296,6 @@
                 "fastqc_for_fastp",
                 "fastqc_for_fastp/test_trimmed.html",
                 "fastqc_for_fastp/test_trimmed.zip",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1328,6 +1319,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1336,7 +1328,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:31:12.481382473"
+        "timestamp": "2025-09-04T17:52:24.935928"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1395,8 +1387,6 @@
                 "fusioncatcher/test.fusion-genes.txt",
                 "fusioncatcher/test.log",
                 "fusioncatcher/test.summary.txt",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1420,6 +1410,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai"
             ]
@@ -1428,7 +1419,7 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:28:10.779582543"
+        "timestamp": "2025-09-04T17:43:49.673677"
     },
     "stub test all": {
         "content": [
@@ -1597,8 +1588,6 @@
                 "fusionreport/test/test.fusions.csv",
                 "fusionreport/test/test.fusions.json",
                 "fusionreport/test/test_fusionreport_index.html",
-                "gatk4",
-                "gatk4/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "multiqc",
                 "multiqc/multiqc_data",
                 "multiqc/multiqc_plots",
@@ -1629,6 +1618,7 @@
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.genepred",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.interval_list",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46.gtf.refflat",
+                "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.dict",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa",
                 "references/GRCh38/gencode_v46/gencode/Homo_sapiens.GRCh38.46_dna_primary_assembly.fa.fai",
                 "references/GRCh38/gencode_v46/salmon",
@@ -1774,6 +1764,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:26:08.667543202"
+        "timestamp": "2025-09-04T17:39:00.417725"
     }
 }
\ No newline at end of file

From 261d8d87f35faa476d71871fda60541e02dc5821 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 5 Sep 2025 12:49:34 +0200
Subject: [PATCH 847/887] update qc workflow test

---
 subworkflows/local/qc_workflow/main.nf        |  6 ++---
 .../local/qc_workflow/test/main.nf.test       | 24 ++++++++++---------
 2 files changed, 16 insertions(+), 14 deletions(-)

diff --git a/subworkflows/local/qc_workflow/main.nf b/subworkflows/local/qc_workflow/main.nf
index a423471db..d83fea35c 100644
--- a/subworkflows/local/qc_workflow/main.nf
+++ b/subworkflows/local/qc_workflow/main.nf
@@ -19,9 +19,9 @@ workflow QC_WORKFLOW {
 
         PICARD_COLLECTRNASEQMETRICS(
             ch_bam_sorted,
-            ch_refflat.map{ _meta, refflat -> [ refflat ] },
-            ch_fasta.map{ _meta, fasta -> [ fasta ] },
-            ch_rrna_interval.map{ _meta, intervals -> [ intervals ] }.ifEmpty([])
+            ch_refflat.map{ _meta, refflat -> refflat },
+            ch_fasta.map{ _meta, fasta -> fasta  },
+            ch_rrna_interval.map{ _meta, intervals -> intervals }
         ) // Some chromosome or annotation may not have rRNA genes
         ch_versions = ch_versions.mix(PICARD_COLLECTRNASEQMETRICS.out.versions)
         ch_rnaseq_metrics = PICARD_COLLECTRNASEQMETRICS.out.metrics
diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index 2abb710e1..ba3053e95 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -13,8 +13,8 @@ nextflow_workflow {
         setup {
 
             // Create refflat reference
-            run("GTF_TO_REFFLAT") {
-                script "../../../../modules/local/uscs/custom_gtftogenepred/main.nf"
+            run("UCSC_GTFTOGENEPRED") {
+                script "../../../../modules/nf-core/ucsc/gtftogenepred/main.nf"
                 process {
                     """
                     input[0] =Channel.of(
@@ -34,8 +34,8 @@ nextflow_workflow {
                     """
                     input[0] = Channel.of(
                         [
-                            [id: 'fasta'],
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExist: true)
+                            [id: 'gtf'],
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.gtf", checkIfExists: true)
                         ]
                     )
                     """
@@ -60,8 +60,8 @@ nextflow_workflow {
                     input[0] = AGAT_CONVERTGFF2BED.out.bed
                     input[1] = Channel.of(
                         [
-                            [id: 'chr22_dic'],
-                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.dict", checkIfExist: true)
+                            [id: 'chr22_dict'],
+                            file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.dict", checkIfExists: true)
                         ]
                     )
                     """
@@ -75,8 +75,9 @@ nextflow_workflow {
             params {
                 skip_qc              = false
                 fusioninspector_only = false
-                starfusion           = true
-                all                  = true
+                tools                = "starfusion"
+                outdir               = "test"
+
             }
 
             workflow {
@@ -89,12 +90,12 @@ nextflow_workflow {
                     ])
 
                 // ch_refflat
-                input[1] = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
+                input[1] = UCSC_GTFTOGENEPRED.out.refflat
                 // ch_fasta
                 input[2] = Channel.of(
                     [
                         [ id: "test_ref" ],
-                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta", checkIfExist: true)
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta", checkIfExists: true)
                     ]
                 )
 
@@ -102,12 +103,13 @@ nextflow_workflow {
                 input[3] = Channel.of(
                     [
                         [ id: "test_ref" ],
-                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai", checkIfExist: true)
+                        file("https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai", checkIfExists: true)
                     ] )
 
                 // ch rRNA interval list
                 input[4] = GATK4_BEDTOINTERVALLIST.out.interval_list
                 """
+
             }
         }
 

From 3901adb69e81399209598d74b7679cefddb3df21 Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Fri, 5 Sep 2025 12:01:55 +0100
Subject: [PATCH 848/887] feat: add missing PDF and HTML report patterns to
 tower.yml

- Add FastQC HTML reports for both original and trimmed reads
- Add FusionInspector web HTML report pattern
- Add individual fusion report HTML files from fusionreport
- Add MultiQC HTML report and PDF plots patterns
- Add pipeline execution and DAG HTML reports
- Update Picard PDF pattern to be more general
- Fix VCF pattern to match actual file extensions

These changes ensure all PDF and HTML files generated by the pipeline
are properly captured in Tower's report visualization system.
---
 tower.yml | 24 ++++++++++++++++++++----
 1 file changed, 20 insertions(+), 4 deletions(-)

diff --git a/tower.yml b/tower.yml
index 2edf5a7fe..73348eb4b 100644
--- a/tower.yml
+++ b/tower.yml
@@ -7,25 +7,41 @@ reports:
     display: "PDF visualisation of the transcripts involved in predicted fusions"
   "**/fastp/*fastp.html":
     display: "Post fastp trimming HTML report"
+  "**/fastqc/*_fastqc.html":
+    display: "FastQC HTML report"
+  "**/fastqc_for_fastp/*_fastqc.html":
+    display: "FastQC HTML report for trimmed reads"
   "**/fusioncatcher/*.fusioncatcher.fusion-genes.txt":
     display: "FusionCatcher identified fusion TXT report"
+  "**/fusioninspector/*.fusion_inspector_web.html":
+    display: "FusionInspector web HTML report"
   "**/fusioninspector/*.FusionInspector.fusions.abridged.tsv":
     display: "FusionInspector TSV report"
   "**/fusionreport/*/*_fusionreport_index.html":
     display: "Fusion-report HTML report"
-  "**/vcf/*_fusion_data.vcf.gz":
-    display: "Collected statistics on each fusion fed to FusionInspector in VCF format"
+  "**/fusionreport/*/*.html":
+    display: "Individual fusion report HTML files"
+  "**/multiqc/multiqc_report.html":
+    display: "MultiQC HTML report"
+  "**/multiqc/multiqc_plots/pdf/*.pdf":
+    display: "MultiQC PDF plots"
+  "**/pipeline_info/execution_report_*.html":
+    display: "Pipeline execution report HTML"
+  "**/pipeline_info/pipeline_dag_*.html":
+    display: "Pipeline DAG HTML"
   "**/picard/*.MarkDuplicates.metrics.txt":
     display: "Picard: Metrics from CollectRnaMetrics"
   "**/picard/*_rna_metrics.txt":
     display: "GATK4: Metrics from MarkDuplicates"
   "**/picard/*insert*size*metrics.txt":
     display: "GATK4: Metrics from InsertSizeMetrics"
-  "**/picard/*pdf":
-    display: "GATK4: InsertSizeMetrics histogram"
+  "**/picard/*.pdf":
+    display: "Picard PDF reports"
   "**/star_for_starfusion/*ReadsPerGene.out.tab":
     display: "Number of reads per gene"
   "**/starfusion/*.starfusion.fusion_predictions.tsv":
     display: "STAR-Fusion identified fusion TSV report"
   "**/stringtie/*/*stringtie.merged.gtf":
     display: "Merged GTFs from StringTie with annotations"
+  "**/vcf/*_fusion_data.vcf":
+    display: "Collected statistics on each fusion fed to FusionInspector in VCF format"

From a8262a814f6eeae208784fb5453113a0e0a547d4 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 5 Sep 2025 13:02:24 +0200
Subject: [PATCH 849/887] update build snap

---
 tests/test_build.nf.test.snap | 7 +++----
 1 file changed, 3 insertions(+), 4 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 7e0e54ae2..722144ae1 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -55,8 +55,6 @@
                 "agat/minigenome.gtf.bed",
                 "bioawk",
                 "bioawk/minigenome.gtf_rrna.gtf.gz",
-                "gatk4",
-                "gatk4/minigenome.dict",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
@@ -68,6 +66,7 @@
                 "references/GRCh38/fusion_report_db/mitelman.db",
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/minigenome.dict",
                 "references/GRCh38/gencode_v46/gencode/minigenome.fa.fai",
                 "references/GRCh38/gencode_v46/gencode/minigenome.gtf.fasta",
                 "references/GRCh38/gencode_v46/gencode/minigenome.gtf.genepred",
@@ -194,8 +193,8 @@
             [
                 "minigenome.gtf.bed:md5,4c881266e0a1b1a41815d05a8055aa40",
                 "minigenome.gtf_rrna.gtf.gz:md5,fb03ec66f68bc4becc71c299ed38b62d",
-                "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
                 "minigenome.gtf.genepred:md5,c5b0d2118efd89f7a2831e2dc486b999",
@@ -287,6 +286,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:18:01.248835367"
+        "timestamp": "2025-09-05T12:58:07.878931"
     }
 }
\ No newline at end of file

From 49c42310a005fddb8be16a0cc3d8180fd327a226 Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Fri, 5 Sep 2025 12:05:18 +0100
Subject: [PATCH 850/887] Remove MultiQC PDF plots pattern

Individual PDF plots are not useful as standalone reports in Tower.
---
 tower.yml | 2 --
 1 file changed, 2 deletions(-)

diff --git a/tower.yml b/tower.yml
index 73348eb4b..fed63f3ad 100644
--- a/tower.yml
+++ b/tower.yml
@@ -23,8 +23,6 @@ reports:
     display: "Individual fusion report HTML files"
   "**/multiqc/multiqc_report.html":
     display: "MultiQC HTML report"
-  "**/multiqc/multiqc_plots/pdf/*.pdf":
-    display: "MultiQC PDF plots"
   "**/pipeline_info/execution_report_*.html":
     display: "Pipeline execution report HTML"
   "**/pipeline_info/pipeline_dag_*.html":

From 7dfe4ebcf7085ad9db90611588d15bb193edfd4b Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Fri, 5 Sep 2025 12:11:39 +0100
Subject: [PATCH 851/887] Remove duplicate MultiQC report pattern

The original multiqc_report.html pattern already covers this file.
---
 tower.yml | 2 --
 1 file changed, 2 deletions(-)

diff --git a/tower.yml b/tower.yml
index fed63f3ad..5f4896145 100644
--- a/tower.yml
+++ b/tower.yml
@@ -21,8 +21,6 @@ reports:
     display: "Fusion-report HTML report"
   "**/fusionreport/*/*.html":
     display: "Individual fusion report HTML files"
-  "**/multiqc/multiqc_report.html":
-    display: "MultiQC HTML report"
   "**/pipeline_info/execution_report_*.html":
     display: "Pipeline execution report HTML"
   "**/pipeline_info/pipeline_dag_*.html":

From 44e9277ac4b3db7acf39bd08afc50ebeeb2b4c37 Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Fri, 5 Sep 2025 12:13:07 +0100
Subject: [PATCH 852/887] Remove redundant fusionreport index pattern

The broader fusionreport/*/*.html pattern already covers the index file.
---
 tower.yml | 4 +---
 1 file changed, 1 insertion(+), 3 deletions(-)

diff --git a/tower.yml b/tower.yml
index 5f4896145..d8351c979 100644
--- a/tower.yml
+++ b/tower.yml
@@ -17,10 +17,8 @@ reports:
     display: "FusionInspector web HTML report"
   "**/fusioninspector/*.FusionInspector.fusions.abridged.tsv":
     display: "FusionInspector TSV report"
-  "**/fusionreport/*/*_fusionreport_index.html":
-    display: "Fusion-report HTML report"
   "**/fusionreport/*/*.html":
-    display: "Individual fusion report HTML files"
+    display: "Fusion-report HTML files"
   "**/pipeline_info/execution_report_*.html":
     display: "Pipeline execution report HTML"
   "**/pipeline_info/pipeline_dag_*.html":

From fc01d42489f6f542fbaf37a79643853b4bee5c61 Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Fri, 5 Sep 2025 12:15:19 +0100
Subject: [PATCH 853/887] Add missing PDF and HTML report patterns to tower.yml

- Add FastQC HTML reports for original and trimmed reads
- Add FusionInspector web HTML report
- Add individual fusion report HTML files
- Add pipeline execution and DAG HTML reports
- Update Picard PDF pattern to be more general
- Fix VCF pattern to match actual file extensions
---
 tower.yml | 8 +++++---
 1 file changed, 5 insertions(+), 3 deletions(-)

diff --git a/tower.yml b/tower.yml
index d8351c979..e47a82250 100644
--- a/tower.yml
+++ b/tower.yml
@@ -17,12 +17,16 @@ reports:
     display: "FusionInspector web HTML report"
   "**/fusioninspector/*.FusionInspector.fusions.abridged.tsv":
     display: "FusionInspector TSV report"
+  "**/fusionreport/*/*_fusionreport_index.html":
+    display: "Fusion-report HTML report"
   "**/fusionreport/*/*.html":
-    display: "Fusion-report HTML files"
+    display: "Individual fusion report HTML files"
   "**/pipeline_info/execution_report_*.html":
     display: "Pipeline execution report HTML"
   "**/pipeline_info/pipeline_dag_*.html":
     display: "Pipeline DAG HTML"
+  "**/vcf/*_fusion_data.vcf":
+    display: "Collected statistics on each fusion fed to FusionInspector in VCF format"
   "**/picard/*.MarkDuplicates.metrics.txt":
     display: "Picard: Metrics from CollectRnaMetrics"
   "**/picard/*_rna_metrics.txt":
@@ -37,5 +41,3 @@ reports:
     display: "STAR-Fusion identified fusion TSV report"
   "**/stringtie/*/*stringtie.merged.gtf":
     display: "Merged GTFs from StringTie with annotations"
-  "**/vcf/*_fusion_data.vcf":
-    display: "Collected statistics on each fusion fed to FusionInspector in VCF format"

From 38f5832b5735eb9ec8f188f20c5f3e80e066190f Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Fri, 5 Sep 2025 13:15:50 +0200
Subject: [PATCH 854/887] prettier

---
 docs/output.md | 1 -
 1 file changed, 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index e2a5c4391..9937f7fb4 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -283,7 +283,6 @@ The weights for databases are as follows:
 The Fusion Indication Index FII is calculated using two components:
 
 1. Tool Detection (50% of total FII)
-
    - Calculated as: (number of tools detecting the fusion) / (number of tools actually used)
    - This reflects how many of the active tools found the fusion
 

From b1c98ff2abd962389a90d3ca56b03d44dcc9c05d Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Fri, 5 Sep 2025 12:16:22 +0100
Subject: [PATCH 855/887] Revert Picard PDF display name change

Keep original display name for existing pattern.
---
 tower.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tower.yml b/tower.yml
index e47a82250..acb6daff4 100644
--- a/tower.yml
+++ b/tower.yml
@@ -34,7 +34,7 @@ reports:
   "**/picard/*insert*size*metrics.txt":
     display: "GATK4: Metrics from InsertSizeMetrics"
   "**/picard/*.pdf":
-    display: "Picard PDF reports"
+    display: "GATK4: InsertSizeMetrics histogram"
   "**/star_for_starfusion/*ReadsPerGene.out.tab":
     display: "Number of reads per gene"
   "**/starfusion/*.starfusion.fusion_predictions.tsv":

From 126c07ee453712e663afd156cf997eca523b9b24 Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Fri, 5 Sep 2025 12:17:29 +0100
Subject: [PATCH 856/887] Revert unnecessary Picard PDF pattern change

The original *pdf pattern already works fine for matching .pdf files.
---
 tower.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tower.yml b/tower.yml
index acb6daff4..be938b3cb 100644
--- a/tower.yml
+++ b/tower.yml
@@ -33,7 +33,7 @@ reports:
     display: "GATK4: Metrics from MarkDuplicates"
   "**/picard/*insert*size*metrics.txt":
     display: "GATK4: Metrics from InsertSizeMetrics"
-  "**/picard/*.pdf":
+  "**/picard/*pdf":
     display: "GATK4: InsertSizeMetrics histogram"
   "**/star_for_starfusion/*ReadsPerGene.out.tab":
     display: "Number of reads per gene"

From 73113eb715ef60019887d966f871822fa3e71a99 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 9 Sep 2025 09:59:08 +0200
Subject: [PATCH 857/887] fix fusioninspector workflow

---
 modules/local/vcf_collect/main.nf             |  1 -
 .../local/fusioninspector_workflow/main.nf    | 13 ++++---
 .../tests/main.nf.test                        | 35 +++++++++----------
 .../tests/main.nf.test.snap                   | 25 ++++---------
 .../tests/nextflow.config                     |  8 -----
 5 files changed, 30 insertions(+), 52 deletions(-)

diff --git a/modules/local/vcf_collect/main.nf b/modules/local/vcf_collect/main.nf
index 2968c67b3..e43a11711 100644
--- a/modules/local/vcf_collect/main.nf
+++ b/modules/local/vcf_collect/main.nf
@@ -21,7 +21,6 @@ process VCF_COLLECT {
 
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    // TODO use BGZIP to compress the VCF file instead of GZIP
     """
     vcf_collect.py --fusioninspector $fusioninspector_tsv --fusionreport $fusionreport_report --fusioninspector_gtf $fusioninspector_gtf_tsv --fusionreport_csv $fusionreport_csv --hgnc $hgnc_ref --sample ${prefix} --out ${prefix}_fusion_data.vcf
 
diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index 300840069..de07933d2 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -31,7 +31,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
         if (whitelist)  {
             ch_whitelist = ch_fusion_list.combine(Channel.value(file(whitelist, checkIfExists:true)))
-                            .map { meta, fusions, whitelist -> [ meta, [fusions, whitelist] ] }
+                            .map { meta, fusions, whitelist_file -> [ meta, [fusions, whitelist_file] ] }
 
             CAT_CAT(ch_whitelist) // fusioninspector takes care of possible duplicates
             ch_versions = ch_versions.mix(CAT_CAT.out.versions)
@@ -47,7 +47,9 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         def tsv_nonempty = FUSIONINSPECTOR.out.tsv.filter { _meta, file -> file.exists() && file.size() > 0 }
         def tsv_abridged_nonempty = FUSIONINSPECTOR.out.abridged_tsv.filter { _meta, file -> file.exists() && file.size() > 0 }
         def gtf_nonempty = FUSIONINSPECTOR.out.out_gtf.filter { _meta, file -> file.exists() && file.size() > 0 }
-
+        if (!tsv_nonempty) {
+            log.warn("FUSIONINSPECTOR confirmed no fusions, skipping VCF and visualisation steps.")
+        }
         if (
             !skip_vcf
         ) {
@@ -61,7 +63,10 @@ workflow FUSIONINSPECTOR_WORKFLOW {
 
             VCF_COLLECT(fusion_data, ch_hgnc_ref, ch_hgnc_date)
             ch_versions = ch_versions.mix(VCF_COLLECT.out.versions)
-
+        }
+        if (
+            !skip_vis
+        ){
             ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(tsv_nonempty)
             ARRIBA_VISUALISATION(
                 ch_bam_sorted_indexed_fusions,
@@ -71,8 +76,6 @@ workflow FUSIONINSPECTOR_WORKFLOW {
             )
             ch_versions = ch_versions.mix(ARRIBA_VISUALISATION.out.versions)
             ch_arriba_visualisation = ARRIBA_VISUALISATION.out.pdf
-        } else {
-            log.warn("FUSIONINSPECTOR confirmed no fusions, skipping VCF and visualisation steps.")
         }
 
     emit:
diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
index e02352e94..37034d577 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test
@@ -74,11 +74,11 @@ nextflow_workflow {
         when {
             params {
                 no_cosmic                  = true
-                whitelist                  = []
+                whitelist                  = ""
                 outdir                     = "test"
                 genomes_base               = "test"
                 fusionreport_download_args = '--no-cosmic'
-                fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
+                fusionreport_detect_args   = "--no-cosmic"
                 fusionreport_detect_args2  = "--export csv"
             }
             workflow {
@@ -94,23 +94,22 @@ nextflow_workflow {
                 input[2] = Channel.of([ [id:'test_sample'], file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")])
                 input[3] = FUSIONREPORT_DETECT.out.report
                 input[4] = FUSIONREPORT_DETECT.out.csv
-                input[5] = Channel.of([
-                    [id:'test_sample'],
-                    [],
-                    []
+                input[5] = Channel.of([[ id:'test', single_end:false ], // meta map
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam", checkIfExists: true),
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai", checkIfExists: true)
                 ])
                 input[6] = Channel.of(
                     [ [ id:'test_gtf' ],
                     file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.gtf')])
-                input[7] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"))
-                input[8] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"))
+                input[7] = Channel.fromPath("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3")
+                input[8] = Channel.fromPath("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv")
                 input[9] = Channel.of([[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")] )
                 input[10] = Channel.of([[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")] )
                 input[11] = STARFUSION_BUILD.out.reference
                 input[12] = false
                 input[13] = true
                 input[14] = 1
-                input[15] = params.whitelist
+                input[15] = null
                 """
             }
         }
@@ -118,7 +117,6 @@ nextflow_workflow {
         then {
             assertAll(
                 { assert workflow.success },
-                { assert snapshot(file(workflow.out.ch_arriba_visualisation[0][1]).length()) },
                 { assert snapshot(workflow.out.versions).match() }
             )
         }
@@ -183,11 +181,11 @@ nextflow_workflow {
         when {
             params {
                 no_cosmic                  = true
-                whitelist                  = []
+                whitelist                  = ""
                 outdir                     = "test"
                 genomes_base               = "test"
                 fusionreport_download_args = '--no-cosmic'
-                fusionreport_detect_args   = {params.no_cosmic} ? "--no-cosmic" : ""
+                fusionreport_detect_args   = "--no-cosmic"
                 fusionreport_detect_args2  = "--export csv"
             }
             workflow {
@@ -203,23 +201,22 @@ nextflow_workflow {
                 input[2] = Channel.of([ [id:'test_sample'], file("https://github.com/FusionInspector/FusionInspector/raw/master/test/fusion_targets.A.txt")])
                 input[3] = FUSIONREPORT_DETECT.out.report
                 input[4] = FUSIONREPORT_DETECT.out.csv
-                input[5] = Channel.of([
-                    [id:'test_sample'],
-                    [],
-                    []
+                input[5] = Channel.of([[ id:'test', single_end:false ], // meta map
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam", checkIfExists: true),
+                    file("https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai", checkIfExists: true)
                 ])
                 input[6] = Channel.of(
                     [ [ id:'test_gtf' ],
                     file('https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/modules/data/genomics/homo_sapiens/genome/minigenome.gtf')])
-                input[7] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3"))
-                input[8] = Channel.of(file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv"))
+                input[7] = Channel.fromPath("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/protein_domains_hg38_GRCh38_v2.5.0.gff3")
+                input[8] = Channel.fromPath("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/arriba/cytobands_hg38_GRCh38_v2.5.0.tsv")
                 input[9] = Channel.of([[ id:'hgnc' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/hgnc_complete_set.txt")] )
                 input[10] = Channel.of([[ id:'hgnc_timestamp' ], file("https://github.com/nf-core/test-datasets/raw/refs/heads/rnafusion/testdata/reference/hgnc/HGNC-DB-timestamp.txt")] )
                 input[11] = STARFUSION_BUILD.out.reference
                 input[12] = false
                 input[13] = true
                 input[14] = 1
-                input[15] = params.whitelist
+                input[15] = null
                 """
             }
         }
diff --git a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
index 8c2578116..f156b0d2e 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusioninspector_workflow/tests/main.nf.test.snap
@@ -3,48 +3,35 @@
         "content": [
             {
                 "0": [
-                    [
-                        {
-                            "id": "test_sample"
-                        },
-                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
+                    
                 ],
                 "1": [
-                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
                     "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
                 ],
                 "ch_arriba_visualisation": [
-                    [
-                        {
-                            "id": "test_sample"
-                        },
-                        "test_sample_combined_fusions_arriba_visualisation.pdf:md5,d41d8cd98f00b204e9800998ecf8427e"
-                    ]
+                    
                 ],
                 "versions": [
-                    "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
                     "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
                 ]
             }
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-26T20:11:13.798144726"
+        "timestamp": "2025-09-08T17:38:59.895142"
     },
     "FUSIONINSPECTOR_WORKFLOW - human": {
         "content": [
             [
-                "versions.yml:md5,01dce7699931b293311f2e5436b88a3a",
                 "versions.yml:md5,3be8e23309ed0bacebcad3d2fb30642a"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-08-28T21:54:05.453749443"
+        "timestamp": "2025-09-08T17:14:05.797063"
     }
 }
\ No newline at end of file
diff --git a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
index e00f3ced2..5dad203b1 100644
--- a/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
+++ b/subworkflows/local/fusioninspector_workflow/tests/nextflow.config
@@ -3,14 +3,6 @@ process {
         ext.args = []
     }
 
-    withName: 'FUSIONREPORT_DOWNLOAD' {
-        ext.args         =  params.fusionreport_download_args
-    }
-
-    withName: 'FUSIONREPORT_DETECT' {
-        ext.args         = params.fusionreport_detect_args
-        ext.args2        = params.fusionreport_detect_args2
-    }
 
     withName: 'FUSIONINSPECTOR' {
         ext.args = '--annotate --examine_coding_effect'

From bd3fddcb454a54f6e13806fe3ab893cc16e73f60 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 9 Sep 2025 13:08:34 +0200
Subject: [PATCH 858/887] update build snap

---
 subworkflows/local/qc_workflow/test/main.nf.test | 4 +---
 tests/test_build.nf.test.snap                    | 7 +++----
 2 files changed, 4 insertions(+), 7 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index ca6657aa0..a68416482 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -71,12 +71,10 @@ nextflow_workflow {
         }
 
         when {
-            // Params to activate modules ext.when condition
             params {
                 skip_qc              = false
                 fusioninspector_only = false
-                starfusion           = true
-                all                  = true
+                tools               = "all"
             }
 
             workflow {
diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 7e0e54ae2..55d1df2b5 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -55,8 +55,6 @@
                 "agat/minigenome.gtf.bed",
                 "bioawk",
                 "bioawk/minigenome.gtf_rrna.gtf.gz",
-                "gatk4",
-                "gatk4/minigenome.dict",
                 "pipeline_info",
                 "pipeline_info/nf_core_rnafusion_software_mqc_versions.yml",
                 "references",
@@ -68,6 +66,7 @@
                 "references/GRCh38/fusion_report_db/mitelman.db",
                 "references/GRCh38/gencode_v46",
                 "references/GRCh38/gencode_v46/gencode",
+                "references/GRCh38/gencode_v46/gencode/minigenome.dict",
                 "references/GRCh38/gencode_v46/gencode/minigenome.fa.fai",
                 "references/GRCh38/gencode_v46/gencode/minigenome.gtf.fasta",
                 "references/GRCh38/gencode_v46/gencode/minigenome.gtf.genepred",
@@ -194,8 +193,8 @@
             [
                 "minigenome.gtf.bed:md5,4c881266e0a1b1a41815d05a8055aa40",
                 "minigenome.gtf_rrna.gtf.gz:md5,fb03ec66f68bc4becc71c299ed38b62d",
-                "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
+                "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
                 "minigenome.gtf.genepred:md5,c5b0d2118efd89f7a2831e2dc486b999",
@@ -287,6 +286,6 @@
             "nf-test": "0.9.2",
             "nextflow": "25.04.6"
         },
-        "timestamp": "2025-09-02T08:18:01.248835367"
+        "timestamp": "2025-09-09T11:45:13.886497"
     }
 }
\ No newline at end of file

From 82608dd0633b694822632c4c881faf6f367f37ed Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 9 Sep 2025 13:41:04 +0200
Subject: [PATCH 859/887] qc test fixes

---
 subworkflows/local/qc_workflow/test/main.nf.test | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/qc_workflow/test/main.nf.test b/subworkflows/local/qc_workflow/test/main.nf.test
index a68416482..0a8eadf95 100644
--- a/subworkflows/local/qc_workflow/test/main.nf.test
+++ b/subworkflows/local/qc_workflow/test/main.nf.test
@@ -13,8 +13,8 @@ nextflow_workflow {
         setup {
 
             // Create refflat reference
-            run("GTF_TO_REFFLAT") {
-                script "../../../../modules/local/uscs/custom_gtftogenepred/main.nf"
+            run("UCSC_GTFTOGENEPRED") {
+                script "../../../../modules/nf-core/ucsc/gtftogenepred/main.nf"
                 process {
                     """
                     input[0] =Channel.of(
@@ -87,7 +87,7 @@ nextflow_workflow {
                     ])
 
                 // ch_refflat
-                input[1] = GTF_TO_REFFLAT.out.refflat.map { that -> [[id:that.Name], that] }
+                input[1] = UCSC_GTFTOGENEPRED.out.refflat
                 // ch_fasta
                 input[2] = Channel.of(
                     [

From 3656a7265a26afce557d4c7207d2a5f32ac6dfc7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 9 Sep 2025 13:43:01 +0200
Subject: [PATCH 860/887] update changelog

---
 CHANGELOG.md | 3 ++-
 1 file changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 15da8cb59..33b18a92c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -96,7 +96,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Changed local `ARRIBA_VISUALIZATION`, `CTATSPLICING_STARTOCANCERINTRONS`, `CTATSPLICING_PREPGENOMELIB`, `FUSIONINSPECTOR`, `STARFUSION_DETECT` for its nf-core module versions [#740](https://github.com/nf-core/rnafusion/pull/740)
 - Replaced local subworkflow `TRIM_WORKFLOW` for its nf-core subworkflow equivalent `FASTQ_FASTQC_UMITOOLS_FASTP` [#752](https://github.com/nf-core/rnafusion/pull/752)
 - Changed local `FASTQ_ALIGN_STAR` for subworkflow from nf-core [#756](https://github.com/nf-core/rnafusion/pull/756)
-- Replaced local subworkflow `STRINGTIE_WORKFLOW` for its nf-core version `BAM_STRINGTIE_MERGE` and the local module module `GTF_TO_REFFLAT` for nf-core's `UCSC_GTFTOGENEPRED` [#758](https://github.com/nf-core/rnafusion/pull/758)
+- Replaced local subworkflow `STRINGTIE_WORKFLOW` for its nf-core version `BAM_STRINGTIE_MERGE` and the local module module `GTF_TO_REFFLAT` for nf-core's `UCSC_GTFTOGENEPRED` [#758](https://github.com/nf-core/rnafusion/pull/758
+- Update modules and close todos as preparation for release [#759](https://github.com/nf-core/rnafusion/pull/759)
 
 ### Fixed
 

From 94a3ef26b7df498353b6b26186fea54765f9dfcb Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 9 Sep 2025 16:28:45 +0200
Subject: [PATCH 861/887] fix bed format

---
 conf/modules.config | 5 +++++
 1 file changed, 5 insertions(+)

diff --git a/conf/modules.config b/conf/modules.config
index 69bfa6947..e588e2b9a 100644
--- a/conf/modules.config
+++ b/conf/modules.config
@@ -18,6 +18,11 @@ process {
         saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
     ]
 
+
+    withName: 'AGAT_CONVERTGFF2BED' {
+        ext.args = '--nc filter'
+    }
+
     withName: 'ARRIBA_ARRIBA' {
         publishDir = [
             path: { "${params.outdir}/arriba" },

From 4d6ff580845985f6ba9422fac73d8fb2ac4b55e8 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 9 Sep 2025 16:30:32 +0200
Subject: [PATCH 862/887] update changelog

---
 CHANGELOG.md | 1 +
 1 file changed, 1 insertion(+)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index ebac98c5f..23134fcff 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -124,6 +124,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix rRNA detection and make it more customizable with nf-core modules [#736](https://github.com/nf-core/rnafusion/pull/736)
 - Fix rRNA detection in GTF using `transcript_type` [#749](https://github.com/nf-core/rnafusion/pull/749)
 - Fixed nf-test for `QC_WORKFLOW` subworkflow [#756](https://github.com/nf-core/rnafusion/pull/756)
+- Fixed issue with bed format, modifying AGAT arguments for non-coding CDS [#763](https://github.com/nf-core/rnafusion/pull/763)
 
 ### Removed
 

From a81c9bbd5cc06c52d531e72af68663f112e3b7db Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 9 Sep 2025 16:39:40 +0200
Subject: [PATCH 863/887] update changelog

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 23134fcff..77777376f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -124,7 +124,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix rRNA detection and make it more customizable with nf-core modules [#736](https://github.com/nf-core/rnafusion/pull/736)
 - Fix rRNA detection in GTF using `transcript_type` [#749](https://github.com/nf-core/rnafusion/pull/749)
 - Fixed nf-test for `QC_WORKFLOW` subworkflow [#756](https://github.com/nf-core/rnafusion/pull/756)
-- Fixed issue with bed format, modifying AGAT arguments for non-coding CDS [#763](https://github.com/nf-core/rnafusion/pull/763)
+- Fixed issue with bed creation, modifying AGAT arguments for non-coding CDS [#763](https://github.com/nf-core/rnafusion/pull/763)
 
 ### Removed
 

From 33978a80d5dce45dbcb6eb2f88998619752f50cc Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 10 Sep 2025 09:17:11 +0200
Subject: [PATCH 864/887] fix fusioncatcher hardlinks

---
 CHANGELOG.md                                  |   1 +
 modules.json                                  | 202 +++++++++++++-----
 .../fusioncatcher/environment.yml             |   2 +-
 .../fusioncatcher-fusioncatcher.diff          |  30 +++
 .../fusioncatcher/fusioncatcher/main.nf       |   4 +-
 5 files changed, 187 insertions(+), 52 deletions(-)
 create mode 100644 modules/nf-core/fusioncatcher/fusioncatcher/fusioncatcher-fusioncatcher.diff

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 77777376f..3eaa3c160 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -125,6 +125,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix rRNA detection in GTF using `transcript_type` [#749](https://github.com/nf-core/rnafusion/pull/749)
 - Fixed nf-test for `QC_WORKFLOW` subworkflow [#756](https://github.com/nf-core/rnafusion/pull/756)
 - Fixed issue with bed creation, modifying AGAT arguments for non-coding CDS [#763](https://github.com/nf-core/rnafusion/pull/763)
+- Fixed fusioncatcher hardlink issues in filesystems that don't allow hardlinks [#]()
 
 ### Removed
 
diff --git a/modules.json b/modules.json
index 9505147f7..fdce13a32 100644
--- a/modules.json
+++ b/modules.json
@@ -8,204 +8,292 @@
                     "agat/convertgff2bed": {
                         "branch": "master",
                         "git_sha": "b785bb0b56bb518ab40c41f41d0e8749fb638257",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "025a6f75e1f72e4ab60abb4bd65b3f289d4ad910",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "arriba/visualisation": {
                         "branch": "master",
                         "git_sha": "025a6f75e1f72e4ab60abb4bd65b3f289d4ad910",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "bioawk": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "ctatsplicing/prepgenomelib": {
                         "branch": "master",
                         "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
-                        "installed_by": ["modules"],
+                        "installed_by": [
+                            "modules"
+                        ],
                         "patch": "modules/nf-core/ctatsplicing/prepgenomelib/ctatsplicing-prepgenomelib.diff"
                     },
                     "ctatsplicing/startocancerintrons": {
                         "branch": "master",
                         "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
-                        "installed_by": ["modules"],
+                        "installed_by": [
+                            "modules"
+                        ],
                         "patch": "modules/nf-core/ctatsplicing/startocancerintrons/ctatsplicing-startocancerintrons.diff"
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
+                        "installed_by": [
+                            "fastq_fastqc_umitools_fastp",
+                            "modules"
+                        ]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
+                        "installed_by": [
+                            "fastq_fastqc_umitools_fastp",
+                            "modules"
+                        ]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ],
+                        "patch": "modules/nf-core/fusioncatcher/fusioncatcher/fusioncatcher-fusioncatcher.diff"
                     },
                     "fusioninspector": {
                         "branch": "master",
                         "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fusionreport/detect": {
                         "branch": "master",
                         "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "fusionreport/download": {
                         "branch": "master",
                         "git_sha": "df4b662dae05afb695a4326fc172178b5a7a48ce",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "a4488d3d09244f29fb4606ba4eef85d59dcc6ad8",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/convert": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["bam_sort_stats_samtools", "modules"]
+                        "installed_by": [
+                            "bam_sort_stats_samtools",
+                            "modules"
+                        ]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "e89ff68a46b848c4d8aabb8569767516d46db319",
-                        "installed_by": ["bam_sort_stats_samtools", "modules"]
+                        "installed_by": [
+                            "bam_sort_stats_samtools",
+                            "modules"
+                        ]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["bam_stats_samtools"]
+                        "installed_by": [
+                            "bam_stats_samtools"
+                        ]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["fastq_align_star", "modules"]
+                        "installed_by": [
+                            "fastq_align_star",
+                            "modules"
+                        ]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "starfusion/build": {
                         "branch": "master",
                         "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "starfusion/detect": {
                         "branch": "master",
                         "git_sha": "b0f38d0ea7515484442edc96046858924fc477dc",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["bam_stringtie_merge", "modules"]
+                        "installed_by": [
+                            "bam_stringtie_merge",
+                            "modules"
+                        ]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["bam_stringtie_merge", "modules"]
+                        "installed_by": [
+                            "bam_stringtie_merge",
+                            "modules"
+                        ]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["modules"]
+                        "installed_by": [
+                            "modules"
+                        ]
                     },
                     "umitools/extract": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": ["fastq_fastqc_umitools_fastp"]
+                        "installed_by": [
+                            "fastq_fastqc_umitools_fastp"
+                        ]
                     }
                 }
             },
@@ -214,45 +302,61 @@
                     "bam_sort_stats_samtools": {
                         "branch": "master",
                         "git_sha": "e89ff68a46b848c4d8aabb8569767516d46db319",
-                        "installed_by": ["fastq_align_star"]
+                        "installed_by": [
+                            "fastq_align_star"
+                        ]
                     },
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["bam_sort_stats_samtools"]
+                        "installed_by": [
+                            "bam_sort_stats_samtools"
+                        ]
                     },
                     "bam_stringtie_merge": {
                         "branch": "master",
                         "git_sha": "5ce6cef7d4a100409aa30bca79b5020b6738767c",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "fastq_align_star": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
                         "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "dcd088f483cede0c3df4034d405126f05a764cc7",
-                        "installed_by": ["subworkflows"]
+                        "installed_by": [
+                            "subworkflows"
+                        ]
                     }
                 }
             }
         }
     }
-}
+}
\ No newline at end of file
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/environment.yml b/modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
index 25f7417c5..8651e2af4 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - "bioconda::fusioncatcher=1.33"
+  - "bioconda::fusioncatcher=1.33b"
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/fusioncatcher-fusioncatcher.diff b/modules/nf-core/fusioncatcher/fusioncatcher/fusioncatcher-fusioncatcher.diff
new file mode 100644
index 000000000..49b2daf36
--- /dev/null
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/fusioncatcher-fusioncatcher.diff
@@ -0,0 +1,30 @@
+Changes in component 'nf-core/fusioncatcher/fusioncatcher'
+Changes in 'fusioncatcher/fusioncatcher/main.nf':
+--- modules/nf-core/fusioncatcher/fusioncatcher/main.nf
++++ modules/nf-core/fusioncatcher/fusioncatcher/main.nf
+@@ -4,8 +4,8 @@
+ 
+     conda "${moduleDir}/environment.yml"
+     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+-        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_5':
+-        'biocontainers/fusioncatcher:1.33--hdfd78af_5' }"
++        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33b--hdfd78af_0':
++        'biocontainers/fusioncatcher:1.33b--hdfd78af_0' }"
+ 
+     input:
+     tuple val(meta), path(fastqs)
+
+Changes in 'fusioncatcher/fusioncatcher/environment.yml':
+--- modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
++++ modules/nf-core/fusioncatcher/fusioncatcher/environment.yml
+@@ -4,4 +4,4 @@
+   - conda-forge
+   - bioconda
+ dependencies:
+-  - "bioconda::fusioncatcher=1.33"
++  - "bioconda::fusioncatcher=1.33b"
+
+'modules/nf-core/fusioncatcher/fusioncatcher/meta.yml' is unchanged
+'modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test' is unchanged
+'modules/nf-core/fusioncatcher/fusioncatcher/tests/main.nf.test.snap' is unchanged
+************************************************************
diff --git a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
index 3abd90d70..ae44e6864 100644
--- a/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
+++ b/modules/nf-core/fusioncatcher/fusioncatcher/main.nf
@@ -4,8 +4,8 @@ process FUSIONCATCHER_FUSIONCATCHER {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33--hdfd78af_5':
-        'biocontainers/fusioncatcher:1.33--hdfd78af_5' }"
+        'https://depot.galaxyproject.org/singularity/fusioncatcher:1.33b--hdfd78af_0':
+        'biocontainers/fusioncatcher:1.33b--hdfd78af_0' }"
 
     input:
     tuple val(meta), path(fastqs)

From 317c1a75012741c29546becc7b5401fb37c7d8fa Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 10 Sep 2025 09:18:29 +0200
Subject: [PATCH 865/887] add pr number

---
 CHANGELOG.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3eaa3c160..15afbfdd8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -125,7 +125,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fix rRNA detection in GTF using `transcript_type` [#749](https://github.com/nf-core/rnafusion/pull/749)
 - Fixed nf-test for `QC_WORKFLOW` subworkflow [#756](https://github.com/nf-core/rnafusion/pull/756)
 - Fixed issue with bed creation, modifying AGAT arguments for non-coding CDS [#763](https://github.com/nf-core/rnafusion/pull/763)
-- Fixed fusioncatcher hardlink issues in filesystems that don't allow hardlinks [#]()
+- Fixed fusioncatcher hardlink issues in filesystems that don't allow hardlinks [#764](https://github.com/nf-core/rnafusion/pull/764)
 
 ### Removed
 

From 6fe4a50e5cbfb8385ba91287b32b8d661ce5174f Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 10 Sep 2025 09:21:11 +0200
Subject: [PATCH 866/887] pre-commit

---
 modules.json | 201 +++++++++++++--------------------------------------
 1 file changed, 49 insertions(+), 152 deletions(-)

diff --git a/modules.json b/modules.json
index fdce13a32..b1ddae700 100644
--- a/modules.json
+++ b/modules.json
@@ -8,292 +8,205 @@
                     "agat/convertgff2bed": {
                         "branch": "master",
                         "git_sha": "b785bb0b56bb518ab40c41f41d0e8749fb638257",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "agat/convertspgff2tsv": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/arriba": {
                         "branch": "master",
                         "git_sha": "025a6f75e1f72e4ab60abb4bd65b3f289d4ad910",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "arriba/visualisation": {
                         "branch": "master",
                         "git_sha": "025a6f75e1f72e4ab60abb4bd65b3f289d4ad910",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "bioawk": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "ctatsplicing/prepgenomelib": {
                         "branch": "master",
                         "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
-                        "installed_by": [
-                            "modules"
-                        ],
+                        "installed_by": ["modules"],
                         "patch": "modules/nf-core/ctatsplicing/prepgenomelib/ctatsplicing-prepgenomelib.diff"
                     },
                     "ctatsplicing/startocancerintrons": {
                         "branch": "master",
                         "git_sha": "8476d6a2adf7fb2e2f23229344aeabcd94a14b0b",
-                        "installed_by": [
-                            "modules"
-                        ],
+                        "installed_by": ["modules"],
                         "patch": "modules/nf-core/ctatsplicing/startocancerintrons/ctatsplicing-startocancerintrons.diff"
                     },
                     "fastp": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "fastq_fastqc_umitools_fastp",
-                            "modules"
-                        ]
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fastqc": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "fastq_fastqc_umitools_fastp",
-                            "modules"
-                        ]
+                        "installed_by": ["fastq_fastqc_umitools_fastp", "modules"]
                     },
                     "fusioncatcher/fusioncatcher": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ],
+                        "installed_by": ["modules"],
                         "patch": "modules/nf-core/fusioncatcher/fusioncatcher/fusioncatcher-fusioncatcher.diff"
                     },
                     "fusioninspector": {
                         "branch": "master",
                         "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fusionreport/detect": {
                         "branch": "master",
                         "git_sha": "2737fcc61a0379e6f130de9069cadf77bc8e97ff",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "fusionreport/download": {
                         "branch": "master",
                         "git_sha": "df4b662dae05afb695a4326fc172178b5a7a48ce",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/bedtointervallist": {
                         "branch": "master",
                         "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
                         "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
                         "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "gffread": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
                         "git_sha": "a4488d3d09244f29fb4606ba4eef85d59dcc6ad8",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectinsertsizemetrics": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectrnaseqmetrics": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "picard/collectwgsmetrics": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/index": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "salmon/quant": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/convert": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "samtools/flagstat": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/idxstats": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "samtools/index": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "bam_sort_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/sort": {
                         "branch": "master",
                         "git_sha": "e89ff68a46b848c4d8aabb8569767516d46db319",
-                        "installed_by": [
-                            "bam_sort_stats_samtools",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_sort_stats_samtools", "modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "bam_stats_samtools"
-                        ]
+                        "installed_by": ["bam_stats_samtools"]
                     },
                     "star/align": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "fastq_align_star",
-                            "modules"
-                        ]
+                        "installed_by": ["fastq_align_star", "modules"]
                     },
                     "star/genomegenerate": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "starfusion/build": {
                         "branch": "master",
                         "git_sha": "310a7a59c7f2362d25070e5928f3139f92377eaf",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "starfusion/detect": {
                         "branch": "master",
                         "git_sha": "b0f38d0ea7515484442edc96046858924fc477dc",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "stringtie/merge": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "bam_stringtie_merge",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stringtie_merge", "modules"]
                     },
                     "stringtie/stringtie": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "bam_stringtie_merge",
-                            "modules"
-                        ]
+                        "installed_by": ["bam_stringtie_merge", "modules"]
                     },
                     "ucsc/gtftogenepred": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "modules"
-                        ]
+                        "installed_by": ["modules"]
                     },
                     "umitools/extract": {
                         "branch": "master",
                         "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
-                        "installed_by": [
-                            "fastq_fastqc_umitools_fastp"
-                        ]
+                        "installed_by": ["fastq_fastqc_umitools_fastp"]
                     }
                 }
             },
@@ -302,61 +215,45 @@
                     "bam_sort_stats_samtools": {
                         "branch": "master",
                         "git_sha": "e89ff68a46b848c4d8aabb8569767516d46db319",
-                        "installed_by": [
-                            "fastq_align_star"
-                        ]
+                        "installed_by": ["fastq_align_star"]
                     },
                     "bam_stats_samtools": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "bam_sort_stats_samtools"
-                        ]
+                        "installed_by": ["bam_sort_stats_samtools"]
                     },
                     "bam_stringtie_merge": {
                         "branch": "master",
                         "git_sha": "5ce6cef7d4a100409aa30bca79b5020b6738767c",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "fastq_align_star": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "fastq_fastqc_umitools_fastp": {
                         "branch": "master",
                         "git_sha": "db5a84497b80c4932b4498ec231f19403d19bcff",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
                         "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
                         "git_sha": "dcd088f483cede0c3df4034d405126f05a764cc7",
-                        "installed_by": [
-                            "subworkflows"
-                        ]
+                        "installed_by": ["subworkflows"]
                     }
                 }
             }
         }
     }
-}
\ No newline at end of file
+}

From 51170b69b7d5008d99e24fe2a8b3cba38e02b740 Mon Sep 17 00:00:00 2001
From: Nicolas Vannieuwkerke <nicolas.vannieuwkerke@ugent.be>
Date: Wed, 10 Sep 2025 09:35:31 +0200
Subject: [PATCH 867/887] update snap

---
 .../local/fusioncatcher_workflow/tests/main.nf.test.snap    | 6 +++---
 1 file changed, 3 insertions(+), 3 deletions(-)

diff --git a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
index 2e9553a99..ec8b6cd3d 100644
--- a/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
+++ b/subworkflows/local/fusioncatcher_workflow/tests/main.nf.test.snap
@@ -3,13 +3,13 @@
         "content": [
             [
                 "versions.yml:md5,24a191a82bb19899f88f6493ad4f1b8c",
-                "versions.yml:md5,aeee6dd3b4dcf4cb11af80dfa0a7bf72"
+                "versions.yml:md5,ec8d5c08a6f0c436e14c29931cc92379"
             ]
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
+            "nextflow": "25.04.6"
         },
-        "timestamp": "2025-06-13T16:36:48.833043"
+        "timestamp": "2025-09-10T09:33:14.158266322"
     }
 }
\ No newline at end of file

From 00819649e2298e632a5a3e2a84908385d6988382 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Sep 2025 13:06:25 +0200
Subject: [PATCH 868/887] version bumped to 4.0.0

---
 .nf-core.yml              | 12 +++---
 assets/multiqc_config.yml | 43 +++++++++----------
 nextflow.config           |  2 +-
 ro-crate-metadata.json    | 88 +++++++++++++++++++++++++++++++--------
 4 files changed, 100 insertions(+), 45 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 5f86f8416..2c51645d6 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,11 +1,11 @@
 lint:
   files_unchanged:
-    - .github/CONTRIBUTING.md
-    - .github/PULL_REQUEST_TEMPLATE.md
-    - conf/igenomes.config
-    - conf/igenomes_ignored.config
+  - .github/CONTRIBUTING.md
+  - .github/PULL_REQUEST_TEMPLATE.md
+  - conf/igenomes.config
+  - conf/igenomes_ignored.config
   files_exist:
-    - tests/default.nf.test
+  - tests/default.nf.test
 nf_core_version: 3.3.2
 repository_type: pipeline
 template:
@@ -16,4 +16,4 @@ template:
   name: rnafusion
   org: nf-core
   outdir: .
-  version: 4.0.0dev
+  version: 4.0.0
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 963555cf6..80bf4ed6e 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -1,7 +1,8 @@
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/rnafusion/tree/dev" target="_blank">nf-core/rnafusion</a>
-  analysis pipeline. For information about how to interpret these results, please see the
-  <a href="https://nf-co.re/rnafusion/dev/docs/output" target="_blank">documentation</a>.
+  This report has been generated by the <a href="https://github.com/nf-core/rnafusion/releases/tag/4.0.0"
+  target="_blank">nf-core/rnafusion</a> analysis pipeline. For information about how
+  to interpret these results, please see the <a href="https://nf-co.re/rnafusion/4.0.0/docs/output"
+  target="_blank">documentation</a>.
 
 report_section_order:
   nf-core-rnafusion-methods-description:
@@ -16,23 +17,23 @@ disable_version_detection: true
 
 # Run only these modules
 run_modules:
-  - custom_content
-  - fastqc
-  - fastp
-  - star
-  - samtools
-  - picard
-  - arriba
+- custom_content
+- fastqc
+- fastp
+- star
+- samtools
+- picard
+- arriba
 
 module_order:
-  - fastp
-  - fastqc:
-      name: "FastQC (raw)"
-      info: "This section of the report shows FastQC results before adapter trimming."
-      path_filters:
-        - "*.zip"
-  - fastqc:
-      name: "FastQC (trimmed)"
-      info: "This section of the report shows FastQC results after adapter trimming."
-      path_filters:
-        - "*_trimmed*.zip"
+- fastp
+- fastqc:
+    name: "FastQC (raw)"
+    info: "This section of the report shows FastQC results before adapter trimming."
+    path_filters:
+    - "*.zip"
+- fastqc:
+    name: "FastQC (trimmed)"
+    info: "This section of the report shows FastQC results after adapter trimming."
+    path_filters:
+    - "*_trimmed*.zip"
diff --git a/nextflow.config b/nextflow.config
index bf0f24ca1..42a41c98a 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -347,7 +347,7 @@ manifest {
     mainScript      = 'main.nf'
     defaultBranch   = 'master'
     nextflowVersion = '!>=24.10.5'
-    version         = '4.0.0dev'
+    version         = '4.0.0'
     doi             = ''
 }
 
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index bcd77eb1e..3183bf61a 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -21,16 +21,22 @@
         {
             "@id": "./",
             "@type": "Dataset",
-            "creativeWorkStatus": "InProgress",
-            "datePublished": "2025-07-08T11:39:11+00:00",
+            "creativeWorkStatus": "Stable",
+            "datePublished": "2025-09-10T11:04:42+00:00",
             "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-rnafusion_logo_dark_new.png\">\n    <img alt=\"nf-core/rnafusion\" src=\"docs/images/nf-core-rnafusion_logo_light_new.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.2565517-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.2565517)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A524.10.5-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.3.2-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.3.2)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/rnafusion)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/rnafusion** is a bioinformatics best-practice analysis pipeline for RNA sequencing consisting of several tools designed for detecting and visualizing fusion genes. Results from up to 3 fusion callers tools are created, and are also aggregated, most notably in a pdf visualisation document, a vcf data collection file, and html and tsv reports.\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnafusion/results).\n\nIn rnafusion the full-sized test includes reference building and fusion detection. The test dataset is taken from [here](https://github.com/nf-core/test-datasets/tree/rnafusion/testdata/human).\n\n## Pipeline summary\n\n![nf-core/rnafusion metro map](docs/images/nf-core-rnafusion_metro_map.png)\n\n### References\n\nThe references for the pipeline can be downloaded from the nf-core AWS megatests S3 bucket using the following command for the [AWS CLI tool](https://github.com/aws/aws-cli):\n\n```bash\naws --no-sign-request s3 sync s3://nf-core-awsmegatests/rnafusion/references/ <path_to_references>\n```\n\nThe path to the downloaded references can then be provided to the pipeline with the `--genomes_base` parameter.\n\n\u26a0\ufe0f **Please note that the references are large and can take a long time to download, so it is recommended to download them once and use them for all future runs of the pipeline.**\n\nThe pipeline is also able to build the references in case files from a specific gencode version are missing (Note: only gencode 46 is available for fusioncatcher). This is done automatically when the expected references are not found and these files will be automatically published in the directory specified by the `--genomes_base` parameter. Use the `--references_only` parameter to trigger the reference building workflow only, without running the rest of the pipeline.\n\n1. Download gencode fasta and gtf files\n2. Download the HGNC nomenclature file\n3. Create files needed for QC (Sequence Dictionary and RRNA intervals)\n4. Convert the gtf file to a refflat file\n5. Create the [Salmon](https://salmon.readthedocs.io/en/latest/) index\n6. Create [STAR](https://github.com/alexdobin/STAR) index\n7. Build [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion) and [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING) references\n8. Download [Fusion-report](https://github.com/Clinical-Genomics/fusion-report) DBs\n\n> [!WARNING]\n> References for Fusioncatcher and Arriba cannot be automatically created by the pipeline and should be downloaded from the S3 bucket or another source. See the [References](#references) section for more information.\n\n#### Main workflow\n\n1. Input samplesheet check\n2. Reads quality control ([FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n3. Optional trimming with [fastp](https://github.com/OpenGene/fastp)\n4. Align FASTQs to BAM with [STAR](https://github.com/alexdobin/STAR)\n5. Run fusion detection with [Arriba](https://github.com/suhrig/arriba)\n6. Run fusion detection with [STAR-Fusion](https://github.com/STAR-Fusion/STAR-Fusion)\n   7a. Optional trimming of 3' end with [fastp](https://github.com/OpenGene/fastp) to feed into fusioncatcher (other tools not affected)\n   7b. Run fusion detection with [FusionCatcher](https://github.com/ndaniel/fusioncatcher)\n7. Run transcript assembly and quantification with [StringTie](https://ccb.jhu.edu/software/stringtie/)\n8. Run cancer splicing aberrations detection with [CTAT-SPLICING](https://github.com/TrinityCTAT/CTAT-SPLICING)\n9. Merge all fusions detected by the selected tools with [Fusion-report](https://github.com/Clinical-Genomics/fusion-report)\n10. Post-processing and analysis of data\n    - [FusionInspector](https://github.com/FusionInspector/FusionInspector)\n    - Summarize information into a VCF file\n    - [Arriba](https://github.com/suhrig/arriba) visualisation\n    - Collect metrics ([`picard CollectRnaSeqMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037057492-CollectRnaSeqMetrics-Picard-)), [`picard CollectInsertSizeMetrics`](https://gatk.broadinstitute.org/hc/en-us/articles/360037055772-CollectInsertSizeMetrics-Picard-) and ([`GATK MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-))\n11. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n12. Compress bam files to cram with [samtools view](http://www.htslib.org/)\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nAs the reference building is computationally heavy (> 24h on HPC), we had to use dummy reference files in the test profile. Therefore, it is recommended to run the test profile with the `-stub` option.\n\n```bash\nnextflow run nf-core/rnafusion \\\n   -profile test,<docker/singularity/.../institute> \\\n   --outdir <OUTDIR> \\\n   -stub\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n> **Notes:**\n>\n> - Conda is not currently supported; run with singularity or docker.\n> - Paths need to be absolute.\n> - GRCh38 is the only supported reference.\n> - Single-end reads are to be used as last-resort. Paired-end reads are recommended. FusionCatcher cannot be used with single-end reads shorter than 130 bp.\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/rnafusion/usage) and the [parameter documentation](https://nf-co.re/rnafusion/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/rnafusion/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/rnafusion/output).\n\n## Credits\n\nnf-core/rnafusion was written by Martin Proks ([@matq007](https://github.com/matq007)), Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) and Annick Renevey ([@rannick](https://github.com/rannick))\n\n## We thank the following people for their help in the development of this pipeline\n\n- [Phil Ewels](https://github.com/ewels)\n- [Rickard Hammar\u00e9n](https://github.com/Hammarn)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Praveen Raj](https://github.com/praveenraj2018)\n- [Anabella Trigila](https://github.com/atrigila)\n- [Nicolas Vannieuwkerke](https://github.com/nvnieuwk)\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#rnafusion` channel](https://nfcore.slack.com/channels/rnafusion) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use nf-core/rnafusion for your analysis, please cite it using the following doi: [10.5281/zenodo.3946477](https://doi.org/10.5281/zenodo.3946477)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
                 },
+                {
+                    "@id": "docs/images/nf-core-rnafusion_metro_map.png"
+                },
                 {
                     "@id": "assets/"
                 },
+                {
+                    "@id": "bin/"
+                },
                 {
                     "@id": "conf/"
                 },
@@ -43,6 +49,9 @@
                 {
                     "@id": "modules/"
                 },
+                {
+                    "@id": "modules/local/"
+                },
                 {
                     "@id": "modules/nf-core/"
                 },
@@ -99,7 +108,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#cfd4e34a-0799-4d73-95ca-6b4c84af3e73"
+                    "@id": "#22caf67a-5268-45f3-acca-9cff28a06eda"
                 }
             ],
             "name": "nf-core/rnafusion"
@@ -128,21 +137,30 @@
             ],
             "creator": [
                 {
-                    "@id": "#phil.ewels@scilifelab.se"
+                    "@id": "#max.u.garcia@gmail.com"
                 },
                 {
-                    "@id": "#max.u.garcia@gmail.com"
+                    "@id": "https://orcid.org/0000-0001-7411-6063"
                 },
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
                 },
+                {
+                    "@id": "#phil.ewels@scilifelab.se"
+                },
+                {
+                    "@id": "https://orcid.org/0009-0003-5619-1555"
+                },
                 {
                     "@id": "https://orcid.org/0000-0001-9017-591X"
                 }
             ],
             "dateCreated": "",
-            "dateModified": "2025-07-08T11:39:11Z",
+            "dateModified": "2025-09-10T13:04:42Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
+            "image": {
+                "@id": "docs/images/nf-core-rnafusion_metro_map.png"
+            },
             "keywords": [
                 "nf-core",
                 "nextflow",
@@ -156,8 +174,14 @@
                 "MIT"
             ],
             "maintainer": [
+                {
+                    "@id": "https://orcid.org/0000-0001-7411-6063"
+                },
                 {
                     "@id": "https://orcid.org/0000-0002-8178-3128"
+                },
+                {
+                    "@id": "https://orcid.org/0009-0003-5619-1555"
                 }
             ],
             "name": [
@@ -171,10 +195,10 @@
             },
             "url": [
                 "https://github.com/nf-core/rnafusion",
-                "https://nf-co.re/rnafusion/dev/"
+                "https://nf-co.re/rnafusion/4.0.0/"
             ],
             "version": [
-                "4.0.0dev"
+                "4.0.0"
             ]
         },
         {
@@ -190,11 +214,19 @@
             "version": "!>=24.10.5"
         },
         {
-            "@id": "#cfd4e34a-0799-4d73-95ca-6b4c84af3e73",
+            "@id": "docs/images/nf-core-rnafusion_metro_map.png",
+            "@type": [
+                "File",
+                "ImageObject"
+            ],
+            "name": "Workflow diagram"
+        },
+        {
+            "@id": "#22caf67a-5268-45f3-acca-9cff28a06eda",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#1076262d-a75d-4b99-bdd5-326b8f957f0e"
+                    "@id": "#ee1eeb2c-e0a3-45fc-8770-0e80c9d1489b"
                 }
             ],
             "mainEntity": {
@@ -203,7 +235,7 @@
             "name": "Test suite for nf-core/rnafusion"
         },
         {
-            "@id": "#1076262d-a75d-4b99-bdd5-326b8f957f0e",
+            "@id": "#ee1eeb2c-e0a3-45fc-8770-0e80c9d1489b",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/rnafusion",
             "resource": "repos/nf-core/rnafusion/actions/workflows/nf-test.yml",
@@ -225,6 +257,11 @@
             "@type": "Dataset",
             "description": "Additional files"
         },
+        {
+            "@id": "bin/",
+            "@type": "Dataset",
+            "description": "Scripts that must be callable from a pipeline process"
+        },
         {
             "@id": "conf/",
             "@type": "Dataset",
@@ -245,6 +282,11 @@
             "@type": "Dataset",
             "description": "Modules used by the pipeline"
         },
+        {
+            "@id": "modules/local/",
+            "@type": "Dataset",
+            "description": "Pipeline-specific modules"
+        },
         {
             "@id": "modules/nf-core/",
             "@type": "Dataset",
@@ -331,24 +373,36 @@
             "name": "nf-core",
             "url": "https://nf-co.re/"
         },
-        {
-            "@id": "#phil.ewels@scilifelab.se",
-            "@type": "Person",
-            "email": "phil.ewels@scilifelab.se",
-            "name": "Phil Ewels"
-        },
         {
             "@id": "#max.u.garcia@gmail.com",
             "@type": "Person",
             "email": "max.u.garcia@gmail.com",
             "name": "Maxime Garcia"
         },
+        {
+            "@id": "https://orcid.org/0000-0001-7411-6063",
+            "@type": "Person",
+            "email": "47788523+rannick@users.noreply.github.com",
+            "name": "Annick Renevey"
+        },
         {
             "@id": "https://orcid.org/0000-0002-8178-3128",
             "@type": "Person",
             "email": "mproksik@gmail.com",
             "name": "Martin Proks"
         },
+        {
+            "@id": "#phil.ewels@scilifelab.se",
+            "@type": "Person",
+            "email": "phil.ewels@scilifelab.se",
+            "name": "Phil Ewels"
+        },
+        {
+            "@id": "https://orcid.org/0009-0003-5619-1555",
+            "@type": "Person",
+            "email": "101190534+nvnieuwk@users.noreply.github.com",
+            "name": "Nicolas Vannieuwkerke"
+        },
         {
             "@id": "https://orcid.org/0000-0001-9017-591X",
             "@type": "Person",

From 559c8c603da9c3478482f35edbd479b93c6d109e Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Sep 2025 14:14:20 +0200
Subject: [PATCH 869/887] prettier

---
 .nf-core.yml              | 10 +++++-----
 CHANGELOG.md              |  2 +-
 assets/multiqc_config.yml | 36 ++++++++++++++++++------------------
 3 files changed, 24 insertions(+), 24 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 2c51645d6..4f80b3e13 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,11 +1,11 @@
 lint:
   files_unchanged:
-  - .github/CONTRIBUTING.md
-  - .github/PULL_REQUEST_TEMPLATE.md
-  - conf/igenomes.config
-  - conf/igenomes_ignored.config
+    - .github/CONTRIBUTING.md
+    - .github/PULL_REQUEST_TEMPLATE.md
+    - conf/igenomes.config
+    - conf/igenomes_ignored.config
   files_exist:
-  - tests/default.nf.test
+    - tests/default.nf.test
 nf_core_version: 3.3.2
 repository_type: pipeline
 template:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 15afbfdd8..2e1eb9277 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## v4.0.0dev - [date]
+## v4.0.0 - [2025-09-10]
 
 ### Added
 
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 80bf4ed6e..73d0edd30 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -17,23 +17,23 @@ disable_version_detection: true
 
 # Run only these modules
 run_modules:
-- custom_content
-- fastqc
-- fastp
-- star
-- samtools
-- picard
-- arriba
+  - custom_content
+  - fastqc
+  - fastp
+  - star
+  - samtools
+  - picard
+  - arriba
 
 module_order:
-- fastp
-- fastqc:
-    name: "FastQC (raw)"
-    info: "This section of the report shows FastQC results before adapter trimming."
-    path_filters:
-    - "*.zip"
-- fastqc:
-    name: "FastQC (trimmed)"
-    info: "This section of the report shows FastQC results after adapter trimming."
-    path_filters:
-    - "*_trimmed*.zip"
+  - fastp
+  - fastqc:
+      name: "FastQC (raw)"
+      info: "This section of the report shows FastQC results before adapter trimming."
+      path_filters:
+        - "*.zip"
+  - fastqc:
+      name: "FastQC (trimmed)"
+      info: "This section of the report shows FastQC results after adapter trimming."
+      path_filters:
+        - "*_trimmed*.zip"

From f932e4aff153242a8f44f82052e846618e9e2da5 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 10 Sep 2025 16:20:21 +0200
Subject: [PATCH 870/887] update version in snap

---
 tests/test_build.nf.test.snap    | 10 ++---
 tests/test_stub.nf.test.snap     | 74 ++++++++++++++++----------------
 tests/test_stub_bam.nf.test.snap | 66 ++++++++++++++--------------
 3 files changed, 75 insertions(+), 75 deletions(-)

diff --git a/tests/test_build.nf.test.snap b/tests/test_build.nf.test.snap
index 55d1df2b5..8eb7bdc62 100644
--- a/tests/test_build.nf.test.snap
+++ b/tests/test_build.nf.test.snap
@@ -47,7 +47,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -191,14 +191,14 @@
                 "references/GRCh38/hgnc/hgnc_complete_set.txt"
             ],
             [
-                "minigenome.gtf.bed:md5,4c881266e0a1b1a41815d05a8055aa40",
+                "minigenome.gtf.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.gtf_rrna.gtf.gz:md5,fb03ec66f68bc4becc71c299ed38b62d",
                 "fusion_report.log:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "minigenome.dict:md5,f2dfb8df1d1f860050fc0dba1399fd9e",
                 "minigenome.fa.fai:md5,e3f74a27219b33ae80fd5de5cbeaf32b",
                 "minigenome.gtf.fasta:md5,349d42b5dbd73e163cdbad3453d8cd3a",
                 "minigenome.gtf.genepred:md5,c5b0d2118efd89f7a2831e2dc486b999",
-                "minigenome.gtf.interval_list:md5,6496748a0994cb8a9764761ae74f8004",
+                "minigenome.gtf.interval_list:md5,9111fe876b17e5c18314206e9f985b8c",
                 "minigenome.gtf.refflat:md5,ed76bc063c98087f1100a928923758c7",
                 "complete_ref_lens.bin:md5,3b6d71ddb3eb8239be6623ab350a636d",
                 "ctg_offsets.bin:md5,174ead59c00c5264300d3ff1de673074",
@@ -284,8 +284,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-09T11:45:13.886497"
+        "timestamp": "2025-09-10T15:15:36.32959"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub.nf.test.snap b/tests/test_stub.nf.test.snap
index df001725e..65d5a3c2d 100644
--- a/tests/test_stub.nf.test.snap
+++ b/tests/test_stub.nf.test.snap
@@ -71,7 +71,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -234,9 +234,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:13:34.296716"
+        "timestamp": "2025-09-10T15:26:56.896294"
     },
     "stub test stringtie": {
         "content": [
@@ -308,7 +308,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -398,9 +398,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:15:18.001786"
+        "timestamp": "2025-09-10T15:28:41.962459"
     },
     "stub test arriba": {
         "content": [
@@ -469,7 +469,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -555,9 +555,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:07:39.086364"
+        "timestamp": "2025-09-10T15:21:05.508972"
     },
     "stub test fusioninspector": {
         "content": [
@@ -643,7 +643,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -832,9 +832,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:21:42.479016"
+        "timestamp": "2025-09-10T15:35:06.932469"
     },
     "stub test salmon": {
         "content": [
@@ -874,7 +874,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -927,9 +927,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:19:36.873298"
+        "timestamp": "2025-09-10T15:32:56.766056"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -1006,7 +1006,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1182,9 +1182,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:09:29.991365"
+        "timestamp": "2025-09-10T15:22:53.217808"
     },
     "stub test fusionreport": {
         "content": [
@@ -1203,7 +1203,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
+                    "fusioncatcher": "1.35"
                 },
                 "FUSIONREPORT_DETECT": {
                     "fusion_report": "4.0.1"
@@ -1270,7 +1270,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1454,9 +1454,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:17:14.759248"
+        "timestamp": "2025-09-10T15:30:41.638938"
     },
     "stub test fastp": {
         "content": [
@@ -1493,7 +1493,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1534,9 +1534,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:18:27.651568"
+        "timestamp": "2025-09-10T15:31:48.594211"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1552,7 +1552,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
+                    "fusioncatcher": "1.35"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1570,7 +1570,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1606,9 +1606,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:10:40.658609"
+        "timestamp": "2025-09-10T15:24:00.433475"
     },
     "stub test fusioncatcher trim": {
         "content": [
@@ -1627,7 +1627,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
+                    "fusioncatcher": "1.35"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1645,7 +1645,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1687,9 +1687,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:11:47.659941"
+        "timestamp": "2025-09-10T15:25:10.408652"
     },
     "stub test all": {
         "content": [
@@ -1720,7 +1720,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
+                    "fusioncatcher": "1.35"
                 },
                 "FUSIONINSPECTOR": {
                     "FusionInspector": "2.10.0"
@@ -1808,7 +1808,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -2059,8 +2059,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:05:39.966652"
+        "timestamp": "2025-09-10T15:19:22.837273"
     }
 }
\ No newline at end of file
diff --git a/tests/test_stub_bam.nf.test.snap b/tests/test_stub_bam.nf.test.snap
index c40bcb577..c895d618b 100644
--- a/tests/test_stub_bam.nf.test.snap
+++ b/tests/test_stub_bam.nf.test.snap
@@ -51,7 +51,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -189,9 +189,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:45:42.13657"
+        "timestamp": "2025-09-10T15:43:47.567472"
     },
     "stub test stringtie": {
         "content": [
@@ -243,7 +243,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -311,9 +311,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:47:52.517978"
+        "timestamp": "2025-09-10T15:45:23.291041"
     },
     "stub test arriba": {
         "content": [
@@ -362,7 +362,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -423,9 +423,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:40:41.686721"
+        "timestamp": "2025-09-10T15:38:57.291156"
     },
     "stub test fusioninspector": {
         "content": [
@@ -491,7 +491,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -658,9 +658,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:56:24.707034"
+        "timestamp": "2025-09-10T15:52:15.87533"
     },
     "stub test salmon": {
         "content": [
@@ -710,7 +710,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -772,9 +772,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:54:08.811545"
+        "timestamp": "2025-09-10T15:50:21.195099"
     },
     "stub test ctatsplicing": {
         "content": [
@@ -831,7 +831,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -985,9 +985,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:42:17.062793"
+        "timestamp": "2025-09-10T15:40:36.520194"
     },
     "stub test fusionreport": {
         "content": [
@@ -1006,7 +1006,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
+                    "fusioncatcher": "1.35"
                 },
                 "FUSIONREPORT_DETECT": {
                     "fusion_report": "4.0.1"
@@ -1053,7 +1053,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1212,9 +1212,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:50:22.553497"
+        "timestamp": "2025-09-10T15:47:10.448091"
     },
     "stub test fastp": {
         "content": [
@@ -1261,7 +1261,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1311,9 +1311,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:52:24.935928"
+        "timestamp": "2025-09-10T15:48:46.423679"
     },
     "stub test fusioncatcher": {
         "content": [
@@ -1329,7 +1329,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
+                    "fusioncatcher": "1.35"
                 },
                 "GATK4_BEDTOINTERVALLIST": {
                     "gatk4": "4.6.1.0"
@@ -1357,7 +1357,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1402,9 +1402,9 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:43:49.673677"
+        "timestamp": "2025-09-10T15:42:09.411678"
     },
     "stub test all": {
         "content": [
@@ -1435,7 +1435,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FUSIONCATCHER_FUSIONCATCHER": {
-                    "fusioncatcher": "1.33"
+                    "fusioncatcher": "1.35"
                 },
                 "FUSIONINSPECTOR": {
                     "FusionInspector": "2.10.0"
@@ -1503,7 +1503,7 @@
                     "ucsc": 447
                 },
                 "Workflow": {
-                    "nf-core/rnafusion": "v4.0.0dev"
+                    "nf-core/rnafusion": "v4.0.0"
                 }
             },
             [
@@ -1732,8 +1732,8 @@
         ],
         "meta": {
             "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nextflow": "25.04.7"
         },
-        "timestamp": "2025-09-04T17:39:00.417725"
+        "timestamp": "2025-09-10T15:37:20.367608"
     }
 }
\ No newline at end of file

From e0ca5e7a1c00ab3fe06310bc0990d8df5c364b35 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 11 Sep 2025 14:26:49 +0200
Subject: [PATCH 871/887] remove conda from test on master

---
 .github/workflows/nf-test.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index 8021852c8..e796a814c 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -69,7 +69,7 @@ jobs:
       fail-fast: false
       matrix:
         shard: ${{ fromJson(needs.nf-test-changes.outputs.shard) }}
-        profile: [conda, docker, singularity]
+        profile: [docker, singularity]
         isMain:
           - ${{ github.base_ref == 'master' || github.base_ref == 'main' }}
         # Exclude conda and singularity on dev

From eefca66757b61650d3f73d2ff6f54fc770be4188 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Mon, 15 Sep 2025 12:48:20 +0200
Subject: [PATCH 872/887] update download action: remove non-stub run

---
 .github/workflows/download_pipeline.yml | 7 -------
 1 file changed, 7 deletions(-)

diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 999bcc382..11f0d0cbe 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -96,13 +96,6 @@ jobs:
           NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
           NXF_SINGULARITY_HOME_MOUNT: true
         run: nextflow run ./${{needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
-      - name: Run the downloaded pipeline (stub run not supported)
-        id: run_pipeline
-        if: ${{ steps.stub_run_pipeline.outcome == 'failure' }}
-        env:
-          NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
-          NXF_SINGULARITY_HOME_MOUNT: true
-        run: nextflow run ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -profile test,singularity --outdir ./results
 
       - name: Count the downloaded number of container images
         id: count_afterwards

From 135cd0f521930ea42376f701ba19dbde2c4aaedd Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 16:38:20 +0200
Subject: [PATCH 873/887] Update
 subworkflows/local/fusioninspector_workflow/main.nf

Co-authored-by: Niklas Schandry <schandry@gmail.com>
---
 subworkflows/local/fusioninspector_workflow/main.nf | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/subworkflows/local/fusioninspector_workflow/main.nf b/subworkflows/local/fusioninspector_workflow/main.nf
index de07933d2..6fb7e86d2 100644
--- a/subworkflows/local/fusioninspector_workflow/main.nf
+++ b/subworkflows/local/fusioninspector_workflow/main.nf
@@ -66,7 +66,7 @@ workflow FUSIONINSPECTOR_WORKFLOW {
         }
         if (
             !skip_vis
-        ){
+        ) {
             ch_bam_sorted_indexed_fusions = bam_sorted_indexed.join(tsv_nonempty)
             ARRIBA_VISUALISATION(
                 ch_bam_sorted_indexed_fusions,

From a7f86b7bfb29dce0bded13ba4d3ee147869cf281 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 16:38:33 +0200
Subject: [PATCH 874/887] Update workflows/rnafusion.nf

Co-authored-by: Niklas Schandry <schandry@gmail.com>
---
 workflows/rnafusion.nf | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index eb792032a..678dfad7c 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -188,8 +188,8 @@ workflow RNAFUSION {
             .filter { meta, _fastqs -> meta.align }
 
         // Add the alignment files to the correct channel if their fastqs aren't aligned
-        def ch_aligned_reads        = ch_aligned_inputs
-        def ch_star_junctions       = ch_input.junctions.filter { meta, file -> file && !meta.align }
+        def ch_aligned_reads         = ch_aligned_inputs
+        def ch_star_junctions        = ch_input.junctions.filter { meta, file -> file && !meta.align }
         def ch_star_splice_junctions = ch_input.splice_junctions.filter { meta, file -> file && !meta.align }
         if(tools.intersect(["ctatsplicing", "arriba", "starfusion", "stringtie"])) {
             FASTQ_ALIGN_STAR(

From 64e255e5dc7786cddb6c1a2113d3f4ed7c5d5776 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 16:38:57 +0200
Subject: [PATCH 875/887] Update docs/usage.md

Co-authored-by: Niklas Schandry <schandry@gmail.com>
---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index 0b0220c5d..d8c1903d6 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -280,7 +280,7 @@ nextflow run nf-core/rnafusion \
 --adapter_fasta <PATH/TO/ADAPTER/FASTA> (optional)
 ```
 
-The additional `--trim_tail_fusioncatcher` flag will toggle an additional `fastp` process, especially useful is reads are above 100 bp, which is not handled well by FusionCatcher. The parameter `--trim_tail_fusioncatcher` needs to be provided with the number of bases to remove from the tail end.
+The additional `--trim_tail_fusioncatcher` flag will toggle an additional `fastp` process, especially useful if reads are above 100 bp, which is not handled well by FusionCatcher. The parameter `--trim_tail_fusioncatcher` needs to be provided with the number of bases to remove from the tail end.
 
 #### Filter fusions detected by 2 or more tools
 

From 154f59243ed39e69e2d147eed6409c58cd09a078 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 17:23:51 +0200
Subject: [PATCH 876/887] remove duplicat multiqc

---
 CITATIONS.md | 2 --
 1 file changed, 2 deletions(-)

diff --git a/CITATIONS.md b/CITATIONS.md
index 95bc5d98c..5d2845541 100644
--- a/CITATIONS.md
+++ b/CITATIONS.md
@@ -26,8 +26,6 @@
 
 > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].
 
-- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)
-
 - [FusionCatcher](https://github.com/ndaniel/fusioncatcher)
 
   > Nicorici D, Satalan M, Edgren H, Kangaspeska S, Murumagi A, Kallioniemi O, Virtanen S, Kilkku O. FusionCatcher – a tool for finding somatic fusion genes in paired-end RNA-sequencing data. BioRxiv, 2014 Nov. doi: 10.1101/011650.

From e413486e3f8d02ef4ac4b6b2d05975581a149bbc Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 17:24:20 +0200
Subject: [PATCH 877/887] add author and licence information

---
 bin/vcf_collect.py | 7 +++++++
 1 file changed, 7 insertions(+)

diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py
index bb5401c8c..292c052fd 100755
--- a/bin/vcf_collect.py
+++ b/bin/vcf_collect.py
@@ -1,5 +1,12 @@
 #!/usr/bin/env python3
 
+# Author: Annick Renevey, annick.renevey@scilifelab.se
+# License: MIT
+#
+# This script is part of the rnafusion pipeline.
+# For full license and authorship information, see the repository README.
+#
+
 import argparse
 import logging
 import sys

From 648b5a33f3e9e0f7982948581dd7038cabcf98cf Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 17:32:18 +0200
Subject: [PATCH 878/887] remove unnecessary map

---
 workflows/rnafusion.nf | 3 ---
 1 file changed, 3 deletions(-)

diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf
index eb792032a..e3cf64412 100644
--- a/workflows/rnafusion.nf
+++ b/workflows/rnafusion.nf
@@ -221,9 +221,6 @@ workflow RNAFUSION {
             def ch_ctatsplicing_input = ch_star_splice_junctions
                 .join(ch_star_junctions, failOnMismatch:true, failOnDuplicate:true)
                 .join(ch_aligned_reads, failOnMismatch:true, failOnDuplicate:true)
-                .map { meta, split_junction, junction, bam, bai ->
-                    [ meta, split_junction, junction, bam, bai ]
-                }
 
             CTATSPLICING_STARTOCANCERINTRONS(
                 ch_ctatsplicing_input,

From a601b360ab13605e73206ab47fb4262c639d8dd1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 17:36:47 +0200
Subject: [PATCH 879/887] apply suggestion from code review

---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index 0b0220c5d..30da2bb5f 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -282,7 +282,7 @@ nextflow run nf-core/rnafusion \
 
 The additional `--trim_tail_fusioncatcher` flag will toggle an additional `fastp` process, especially useful is reads are above 100 bp, which is not handled well by FusionCatcher. The parameter `--trim_tail_fusioncatcher` needs to be provided with the number of bases to remove from the tail end.
 
-#### Filter fusions detected by 2 or more tools
+#### Filter for fusions detected by several tools
 
 ```bash
 nextflow run nf-core/rnafusion \

From dfe57813543d3f9136a939efe218ea84566246e7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 17:38:35 +0200
Subject: [PATCH 880/887] add warning about conda

---
 docs/usage.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/usage.md b/docs/usage.md
index 30da2bb5f..24cbdedf3 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -427,7 +427,7 @@ Use this parameter to choose a configuration profile. Profiles can give configur
 Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below.
 
 > [!IMPORTANT]
-> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
+> We highly recommend the use of Docker or Singularity containers, ctatsplicing is not supported by Conda and Conda was not specifically tested for the rest of the pipeline.
 
 The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is supported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
 

From 91a372eb90694d2b78e64d9f2e66b1d812acf462 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 17:39:19 +0200
Subject: [PATCH 881/887] replace by agat

---
 docs/output.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index 9937f7fb4..87b3717a7 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -104,7 +104,7 @@ If no parameters are specified, the default is applied.
 <details markdown="1">
 <summary>Output files</summary>
 
-- `arriba/`
+- `agat/`
   - `<sample>.tsv`
 
 </details>

From 440c67381ae8af98980e7ee2e231584b9d68c2d1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Tue, 16 Sep 2025 17:50:27 +0200
Subject: [PATCH 882/887] remove mentions of conda

---
 .vscode/settings.json | 4 ----
 docs/usage.md         | 4 ++--
 2 files changed, 2 insertions(+), 6 deletions(-)
 delete mode 100644 .vscode/settings.json

diff --git a/.vscode/settings.json b/.vscode/settings.json
deleted file mode 100644
index af2d783fa..000000000
--- a/.vscode/settings.json
+++ /dev/null
@@ -1,4 +0,0 @@
-{
-    "markdown.styles": ["public/vscode_markdown.css"],
-    "nextflow.telemetry.enabled": false
-}
diff --git a/docs/usage.md b/docs/usage.md
index 24cbdedf3..1cade0243 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -480,9 +480,9 @@ To change the resource requests, please see the [max resources](https://nf-co.re
 
 ### Custom Containers
 
-In some cases, you may wish to change the container or conda environment used by a pipeline steps for a particular tool. By default, nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However, in some cases the pipeline specified version maybe out of date.
+In some cases, you may wish to change the container used by a pipeline steps for a particular tool. By default, nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects (via wave for docker and singularity).
 
-To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website.
+To use a different container from the default container specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website.
 
 ### Custom Tool Arguments
 

From 1ce3aaa115356f13db919258eda307697d901542 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 17 Sep 2025 09:33:50 +0200
Subject: [PATCH 883/887] add Delfina Terradas to the contributor list

---
 nextflow.config | 9 +++++++++
 1 file changed, 9 insertions(+)

diff --git a/nextflow.config b/nextflow.config
index 42a41c98a..8f5370a58 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -340,7 +340,16 @@ manifest {
             github: '@nvnieuwk',
             contribution: ['contributor', 'maintainer'],
             orcid: ''
+        ],
+        [
+            name: 'Delfina Terradas',
+            affiliation: 'ZS Associates',
+            email: '',
+            github: '@delfiterradas',
+            contribution: ['contributor'],
+            orcid: ''
         ]
+
     ]
     homePage        = 'https://github.com/nf-core/rnafusion'
     description     = """Nextflow rnafusion analysis pipeline, part of the nf-core community."""

From cea3630a2acf52541090b39bbd4a25952efa0fc1 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Wed, 17 Sep 2025 09:36:27 +0200
Subject: [PATCH 884/887] update description of fastqc and multiqc outputs
 (from template)

---
 docs/output.md | 20 ++++++++++++--------
 1 file changed, 12 insertions(+), 8 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 87b3717a7..3e3171e27 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -200,8 +200,8 @@ If `trim_tail_fusioncatcher` has any value other than 0, [fastp](https://github.
 <summary>Output files</summary>
 
 - `fastqc/`
-  - `*.html`: FastQC report containing quality metrics.
-  - `*.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
+  - `*_fastqc.html`: FastQC report containing quality metrics.
+  - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.
 
 </details>
 
@@ -294,15 +294,19 @@ Final score = (0.5 × Tool Detection Score) + (0.5 × Database Hits Score)
 
 ### MultiQC
 
-![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png)
+<details markdown="1">
+<summary>Output files</summary>
+
+- `multiqc/`
+  - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser.
+  - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline.
+  - `multiqc_plots/`: directory containing static images from the report in various formats.
 
-![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png)
+</details>
 
-![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png)
+[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
 
-:::note
-The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
-:::
+Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
 
 ### Picard
 

From 28742bcf60bec866ec842ec18163170df6e340c7 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 18 Sep 2025 15:48:44 +0200
Subject: [PATCH 885/887] Update docs/output.md

Co-authored-by: Alexander Peltzer <apeltzer@users.noreply.github.com>
---
 docs/output.md | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/docs/output.md b/docs/output.md
index 9937f7fb4..87b3717a7 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -104,7 +104,7 @@ If no parameters are specified, the default is applied.
 <details markdown="1">
 <summary>Output files</summary>
 
-- `arriba/`
+- `agat/`
   - `<sample>.tsv`
 
 </details>

From 3d69168df6ea2e61d5f36afcb23dbdf10caf3308 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 18 Sep 2025 15:50:11 +0200
Subject: [PATCH 886/887] remove commented out outdated comment

---
 docs/usage.md | 4 ----
 1 file changed, 4 deletions(-)

diff --git a/docs/usage.md b/docs/usage.md
index 530fc59dc..0ce3485be 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -83,10 +83,6 @@ nextflow run nf-core/rnafusion \
   --outdir <OUTPUT/PATH> --qiagen
 ```
 
-<!-- #### STAR-Fusion references downloaded vs built
-
-By default STAR-Fusion references are **built**. You can also download them from [CTAT](https://github.com/NCIP/Trinity_CTAT/wiki) by using the flag `--starfusion_build FALSE` for both reference building and fusion detection. This allows more flexibility for different organisms but **be aware that STAR-Fusion reference download is not recommended as not fully tested!** -->
-
 #### Issues with building references
 
 If process `FUSIONREPORT_DOWNLOAD` times out, it could be due to network restriction (for example if trying to run on HPC). As this process is lightweight in cpu, memory and time, running on local machines with the following options might solve the issue:

From bbcb3bd5d1b7ef8e4715aaeb03b5652c29cb7b40 Mon Sep 17 00:00:00 2001
From: Annick Renevey <47788523+rannick@users.noreply.github.com>
Date: Thu, 18 Sep 2025 16:13:26 +0200
Subject: [PATCH 887/887] add info on seq_center and seq_platform

---
 nextflow_schema.json | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/nextflow_schema.json b/nextflow_schema.json
index b6ce64d69..ee0a623e2 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -594,13 +594,13 @@
                 },
                 "seq_center": {
                     "type": "string",
-                    "description": "Sequencing center",
+                    "description": "Sequencing center, used to fill in read group CN tag in the BAM header",
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This will reported in the BAM header as CN"
                 },
                 "seq_platform": {
                     "type": "string",
-                    "description": "Sequencing platform",
+                    "description": "Sequencing platform, used to fill in read group PL tag in the BAM header",
                     "fa_icon": "fas fa-toolbox",
                     "help_text": "This will reported in the BAM header as PL."
                 },