Hi, I am new to Iso-Seq analysis, I want to ask which one should I choose to do the alignment at the first stage: 1. the raw subread data? 2. the ccs data? 3. isoforms produced with PacBio Iso-Seq packages? 4. collapsed isoforms produced by Cupcake-tofu2?
Hi,
I am new to Iso-Seq analysis, I want to ask which one should I choose to do the alignment at the first stage: