diff --git a/docs/tutorial/multiple-values/multiple-options.md b/docs/tutorial/multiple-values/multiple-options.md
index 08c9e80ff2..d6c16b8a92 100644
--- a/docs/tutorial/multiple-values/multiple-options.md
+++ b/docs/tutorial/multiple-values/multiple-options.md
@@ -63,3 +63,49 @@ The sum is 9.5
 ```
 
 </div>
+
+## Passing multiple values in a single argument
+
+**Typer** supports passing multiple arguments with a single option, by using the `separator` parameter in combination with `typing.List[T]` types.
+This feature makes it easy to parse multiple values from a single command-line argument into a list in your application.
+
+To use this feature, define a command-line option that accepts multiple values separated by a specific character (such as a comma). Here's an example of how to implement this:
+
+{* docs_src/multiple_values/multiple_options/tutorial003_an.py hl[10] *}
+
+Check it:
+
+<div class="termy">
+
+```console
+// With no optional CLI argument
+$ python main.py
+
+The sum is 0
+
+// With one number argument
+$ python main.py --number 2
+
+The sum is 2.0
+
+// With several number arguments, split using the separator defined by the Option argument
+$ python main.py --number "2, 3, 4.5"
+
+The sum is 9.5
+
+// You can remove the quotes if no whitespace is added between the numbers
+$ python main.py --number 2,3,4.5
+
+The sum is 9.5
+
+// Supports passing the option multiple times. This joins all values to a single list
+$ python main.py --number 2,3,4.5 --number 5
+
+The sum is 14.5
+```
+
+</div>
+
+/// warning
+
+Only single-character non-whitespace separators are supported.
diff --git a/docs_src/multiple_values/multiple_options/tutorial003.py b/docs_src/multiple_values/multiple_options/tutorial003.py
new file mode 100644
index 0000000000..ba973ad5f9
--- /dev/null
+++ b/docs_src/multiple_values/multiple_options/tutorial003.py
@@ -0,0 +1,14 @@
+from typing import List
+
+import typer
+
+app = typer.Typer()
+
+
+@app.command()
+def main(number: List[float] = typer.Option([], separator=",")):
+    print(f"The sum is {sum(number)}")
+
+
+if __name__ == "__main__":
+    app()
diff --git a/docs_src/multiple_values/multiple_options/tutorial003_an.py b/docs_src/multiple_values/multiple_options/tutorial003_an.py
new file mode 100644
index 0000000000..abdcf4e610
--- /dev/null
+++ b/docs_src/multiple_values/multiple_options/tutorial003_an.py
@@ -0,0 +1,15 @@
+from typing import List
+
+import typer
+from typing_extensions import Annotated
+
+app = typer.Typer()
+
+
+@app.command()
+def main(number: Annotated[List[float], typer.Option(separator=",")] = []):
+    print(f"The sum is {sum(number)}")
+
+
+if __name__ == "__main__":
+    app()
diff --git a/pyproject.toml b/pyproject.toml
index ac89bbd9bb..26e2c31634 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -184,6 +184,7 @@ ignore = [
 # Default mutable data structure
 "docs_src/options_autocompletion/tutorial006_an.py" = ["B006"]
 "docs_src/multiple_values/multiple_options/tutorial002_an.py" = ["B006"]
+"docs_src/multiple_values/multiple_options/tutorial003_an.py" = ["B006"]
 "docs_src/options_autocompletion/tutorial007_an.py" = ["B006"]
 "docs_src/options_autocompletion/tutorial008_an.py" = ["B006"]
 "docs_src/options_autocompletion/tutorial009_an.py" = ["B006"]
diff --git a/tests/test_others.py b/tests/test_others.py
index a8ba207a5f..f1846d3078 100644
--- a/tests/test_others.py
+++ b/tests/test_others.py
@@ -321,3 +321,33 @@ def test_split_opt():
     prefix, opt = _split_opt("verbose")
     assert prefix == ""
     assert opt == "verbose"
+
+
+def test_multiple_options_separator_1_unsupported_separator():
+    app = typer.Typer()
+
+    @app.command()
+    def main(names: typing.List[str] = typer.Option(..., separator="\t \n")):
+        pass  # pragma: no cover
+
+    with pytest.raises(typer.UnsupportedSeparatorError) as exc_info:
+        runner.invoke(app, [])
+    assert (
+        str(exc_info.value)
+        == "Error in definition of Option 'names'. Only single-character non-whitespace separators are supported, but got \"\t \n\"."
+    )
+
+
+def test_multiple_options_separator_2_non_list_type():
+    app = typer.Typer()
+
+    @app.command()
+    def main(names: str = typer.Option(..., separator=",")):
+        pass  # pragma: no cover
+
+    with pytest.raises(typer.SeparatorForNonListTypeError) as exc_info:
+        runner.invoke(app, [])
+    assert (
+        str(exc_info.value)
+        == "Multiple values are supported for List[T] types only. Annotate 'names' as List[str] to support multiple values."
+    )
diff --git a/tests/test_tutorial/test_multiple_values/test_multiple_options/test_tutorial003.py b/tests/test_tutorial/test_multiple_values/test_multiple_options/test_tutorial003.py
new file mode 100644
index 0000000000..d09150eb8d
--- /dev/null
+++ b/tests/test_tutorial/test_multiple_values/test_multiple_options/test_tutorial003.py
@@ -0,0 +1,43 @@
+import subprocess
+import sys
+
+from typer.testing import CliRunner
+
+from docs_src.multiple_values.multiple_options import tutorial003 as mod
+
+app = mod.app
+
+runner = CliRunner()
+
+
+def test_main():
+    result = runner.invoke(app)
+    assert result.exit_code == 0
+    assert "The sum is 0" in result.output
+
+
+def test_1_number():
+    result = runner.invoke(app, ["--number", "2"])
+    assert result.exit_code == 0
+    assert "The sum is 2.0" in result.output
+
+
+def test_2_number():
+    result = runner.invoke(app, ["--number", "2,3,4.5"], catch_exceptions=False)
+    assert result.exit_code == 0
+    assert "The sum is 9.5" in result.output
+
+
+def test_3_number():
+    result = runner.invoke(app, ["--number", "2,3,4.5", "--number", "5"])
+    assert result.exit_code == 0
+    assert "The sum is 14.5" in result.output
+
+
+def test_script():
+    result = subprocess.run(
+        [sys.executable, "-m", "coverage", "run", mod.__file__, "--help"],
+        capture_output=True,
+        encoding="utf-8",
+    )
+    assert "Usage" in result.stdout
diff --git a/tests/test_tutorial/test_multiple_values/test_multiple_options/test_tutorial003_an.py b/tests/test_tutorial/test_multiple_values/test_multiple_options/test_tutorial003_an.py
new file mode 100644
index 0000000000..e7ae10cb28
--- /dev/null
+++ b/tests/test_tutorial/test_multiple_values/test_multiple_options/test_tutorial003_an.py
@@ -0,0 +1,43 @@
+import subprocess
+import sys
+
+from typer.testing import CliRunner
+
+from docs_src.multiple_values.multiple_options import tutorial003_an as mod
+
+app = mod.app
+
+runner = CliRunner()
+
+
+def test_main():
+    result = runner.invoke(app)
+    assert result.exit_code == 0
+    assert "The sum is 0" in result.output
+
+
+def test_1_number():
+    result = runner.invoke(app, ["--number", "2"])
+    assert result.exit_code == 0
+    assert "The sum is 2.0" in result.output
+
+
+def test_2_number():
+    result = runner.invoke(app, ["--number", "2,3,4.5"])
+    assert result.exit_code == 0
+    assert "The sum is 9.5" in result.output
+
+
+def test_3_number():
+    result = runner.invoke(app, ["--number", "2,3,4.5", "--number", "5"])
+    assert result.exit_code == 0
+    assert "The sum is 14.5" in result.output
+
+
+def test_script():
+    result = subprocess.run(
+        [sys.executable, "-m", "coverage", "run", mod.__file__, "--help"],
+        capture_output=True,
+        encoding="utf-8",
+    )
+    assert "Usage" in result.stdout
diff --git a/typer/__init__.py b/typer/__init__.py
index a2e897cdd7..726e03595a 100644
--- a/typer/__init__.py
+++ b/typer/__init__.py
@@ -37,3 +37,9 @@
 from .models import FileTextWrite as FileTextWrite
 from .params import Argument as Argument
 from .params import Option as Option
+from .utils import (
+    SeparatorForNonListTypeError as SeparatorForNonListTypeError,
+)
+from .utils import (
+    UnsupportedSeparatorError as UnsupportedSeparatorError,
+)
diff --git a/typer/core.py b/typer/core.py
index e9631e56cf..aa22a638c6 100644
--- a/typer/core.py
+++ b/typer/core.py
@@ -3,6 +3,7 @@
 import inspect
 import os
 import sys
+import typing as t
 from difflib import get_close_matches
 from enum import Enum
 from gettext import gettext as _
@@ -26,6 +27,7 @@
 import click.shell_completion
 import click.types
 import click.utils
+from click import Context
 
 from ._typing import Literal
 
@@ -446,6 +448,7 @@ def __init__(
         show_envvar: bool = False,
         # Rich settings
         rich_help_panel: Union[str, None] = None,
+        separator: Optional[str] = None,
     ):
         super().__init__(
             param_decls=param_decls,
@@ -475,6 +478,19 @@ def __init__(
         )
         _typer_param_setup_autocompletion_compat(self, autocompletion=autocompletion)
         self.rich_help_panel = rich_help_panel
+        self.original_type = type
+        self.separator = separator
+
+    def _parse_separated_parameter_list(self, parameter_values: List[str]) -> List[str]:
+        values = []
+        for param_str_list in parameter_values:
+            values.extend(param_str_list.split(self.separator))
+        return values
+
+    def process_value(self, ctx: Context, value: t.Any) -> t.Any:
+        if self.separator is not None:
+            value = self._parse_separated_parameter_list(value)
+        return super().process_value(ctx, value)
 
     def _get_default_string(
         self,
diff --git a/typer/main.py b/typer/main.py
index 2e3b1223cc..4429893768 100644
--- a/typer/main.py
+++ b/typer/main.py
@@ -48,7 +48,11 @@
     TyperInfo,
     TyperPath,
 )
-from .utils import get_params_from_function
+from .utils import (
+    SeparatorForNonListTypeError,
+    UnsupportedSeparatorError,
+    get_params_from_function,
+)
 
 _original_except_hook = sys.excepthook
 _typer_developer_exception_attr_name = "__typer_developer_exception__"
@@ -896,6 +900,18 @@ def get_click_param(
             param_decls.extend(parameter_info.param_decls)
         else:
             param_decls.append(default_option_declaration)
+
+        # Check the multiple separator option for validity
+        separator = None
+        if parameter_info.separator:
+            separator = parameter_info.separator.strip()
+
+            if not is_list:
+                raise SeparatorForNonListTypeError(param.name, main_type)
+
+            if len(separator) != 1:
+                raise UnsupportedSeparatorError(param.name, parameter_info.separator)
+
         return (
             TyperOption(
                 # Option
@@ -928,6 +944,7 @@ def get_click_param(
                 autocompletion=get_param_completion(parameter_info.autocompletion),
                 # Rich settings
                 rich_help_panel=parameter_info.rich_help_panel,
+                separator=separator,
             ),
             convertor,
         )
diff --git a/typer/models.py b/typer/models.py
index e0bddb965b..8bbd7f6410 100644
--- a/typer/models.py
+++ b/typer/models.py
@@ -336,6 +336,7 @@ def __init__(
         path_type: Union[None, Type[str], Type[bytes]] = None,
         # Rich settings
         rich_help_panel: Union[str, None] = None,
+        separator: Optional[str] = None,
     ):
         super().__init__(
             default=default,
@@ -398,6 +399,7 @@ def __init__(
         self.hide_input = hide_input
         self.count = count
         self.allow_from_autoenv = allow_from_autoenv
+        self.separator = separator
 
 
 class ArgumentInfo(ParameterInfo):
diff --git a/typer/params.py b/typer/params.py
index 66c2b32d3e..ac3c55962c 100644
--- a/typer/params.py
+++ b/typer/params.py
@@ -202,6 +202,8 @@ def Option(
     path_type: Union[None, Type[str], Type[bytes]] = None,
     # Rich settings
     rich_help_panel: Union[str, None] = None,
+    # Multiple values
+    separator: Optional[str] = None,
 ) -> Any:
     return OptionInfo(
         # Parameter
@@ -257,6 +259,7 @@ def Option(
         path_type=path_type,
         # Rich settings
         rich_help_panel=rich_help_panel,
+        separator=separator,
     )
 
 
diff --git a/typer/utils.py b/typer/utils.py
index 81dc4dd61d..c9a47dbb54 100644
--- a/typer/utils.py
+++ b/typer/utils.py
@@ -188,3 +188,30 @@ def get_params_from_function(func: Callable[..., Any]) -> Dict[str, ParamMeta]:
             name=param.name, default=default, annotation=annotation
         )
     return params
+
+
+class SeparatorForNonListTypeError(Exception):
+    argument_name: str
+    argument_type: Type[Any]
+
+    def __init__(self, argument_name: str, argument_type: Type[Any]):
+        self.argument_name = argument_name
+        self.argument_type = argument_type
+
+    def __str__(self) -> str:
+        return f"Multiple values are supported for List[T] types only. Annotate {self.argument_name!r} as List[{self.argument_type.__name__}] to support multiple values."
+
+
+class UnsupportedSeparatorError(Exception):
+    argument_name: str
+    separator: str
+
+    def __init__(self, argument_name: str, separator: str):
+        self.argument_name = argument_name
+        self.separator = separator
+
+    def __str__(self) -> str:
+        return (
+            f"Error in definition of Option {self.argument_name!r}. "
+            f'Only single-character non-whitespace separators are supported, but got "{self.separator}".'
+        )