diff --git a/app-config.example.yaml b/app-config.example.yaml
index 3dd8ff1..6c6a678 100644
--- a/app-config.example.yaml
+++ b/app-config.example.yaml
@@ -8,14 +8,8 @@ catalog:
   locations:
     - type: file
       target: ../../default-catalog/catalog-info.yaml
-    - type: file
-      target: ../../examples/entities.yaml
-    - type: file
-      target: ../../examples/org.yaml
     - type: file
       target: ../../examples/all.yaml
-    - type: file
-      target: ../../examples/acme-corp.yaml
 ## Uncomment the following to enable Plausible analytics in development mode
 # plausible:
 #   enabled: true
diff --git a/app-config.production.yaml b/app-config.production.yaml
index cd3560c..b6c06d0 100644
--- a/app-config.production.yaml
+++ b/app-config.production.yaml
@@ -47,9 +47,7 @@ auth:
             # See https://backstage.io/docs/auth/github/provider#resolvers for more resolvers.
             - resolver: usernameMatchingUserEntityName
 catalog:
-  # Overrides the default list locations from app-config.yaml as these contain example data.
-  # See https://backstage.io/docs/features/software-catalog/#adding-components-to-the-catalog for more details
-  # on how to get entities into the catalog.
+  # Load sample entities, including guest users, groups and templates, from one location.
   providers:
     github:
       default:
@@ -61,14 +59,8 @@ catalog:
           timeout: { minutes: 3 }
 
   locations:
-    - type: file
-      target: ../../examples/entities.yaml
-    - type: file
-      target: ../../examples/org.yaml
     - type: file
       target: ../../examples/all.yaml
-    - type: file
-      target: ../../examples/acme-corp.yaml
 
 plausible:
   enabled: true
diff --git a/packages/backend/package.json b/packages/backend/package.json
index 8b78e12..8684dad 100644
--- a/packages/backend/package.json
+++ b/packages/backend/package.json
@@ -64,7 +64,8 @@
     "@types/supertest": "^6.0.2",
     "jose": "^5.6.3",
     "msw": "^2.3.4",
-    "supertest": "^7.0.0"
+    "supertest": "^7.0.0",
+    "yaml": "^2.2.2"
   },
   "files": [
     "dist"
diff --git a/packages/backend/src/catalogLocations.test.ts b/packages/backend/src/catalogLocations.test.ts
new file mode 100644
index 0000000..db9adf7
--- /dev/null
+++ b/packages/backend/src/catalogLocations.test.ts
@@ -0,0 +1,31 @@
+import { readFileSync, statSync } from 'node:fs';
+import { resolve } from 'node:path';
+import { ConfigReader } from '@backstage/config';
+import { parse } from 'yaml';
+
+const backendDirectory = resolve(__dirname, '..');
+const repositoryDirectory = resolve(backendDirectory, '../..');
+
+describe.each(['app-config.example.yaml', 'app-config.production.yaml'])(
+  '%s catalog locations',
+  configFile => {
+    const config = new ConfigReader(
+      parse(readFileSync(resolve(repositoryDirectory, configFile), 'utf8')),
+    );
+    const fileLocations = config
+      .getConfigArray('catalog.locations')
+      .filter(location => location.getString('type') === 'file')
+      .map(location => location.getString('target'));
+
+    it('loads the aggregate sample catalog', () => {
+      expect(fileLocations).toContain('../../examples/all.yaml');
+    });
+
+    it.each(fileLocations)('can read the file location %s', target => {
+      // Backstage resolves file locations from the backend package directory.
+      const locationPath = resolve(backendDirectory, target);
+      expect(statSync(locationPath).isFile()).toBe(true);
+      expect(readFileSync(locationPath, 'utf8').trim()).not.toBe('');
+    });
+  },
+);
diff --git a/yarn.lock b/yarn.lock
index 411058e..39c2721 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -16701,6 +16701,7 @@ __metadata:
     pg: "npm:^8.11.3"
     supertest: "npm:^7.0.0"
     winston: "npm:^3.2.1"
+    yaml: "npm:^2.2.2"
   languageName: unknown
   linkType: soft