❓ Question / Help Request
Dear Caffery,
I hope you are well and thank you for the very helpful tool (:
I normally use the output of picrust2 (e.g. pred_metagenome_unstrat.tsv) as the input for ggpicrust2. But I was wondering if we should transform/normalize/standardize the picrust2 output before using it in the ggpicrust2 function. For example, should we transform/normalize it based on the tool we use (Aldex2 vs Deseq2 vs Maaslin2 vs LinDA.
Do you have any recommendations?
Thank you (:
All the best,
Carmen
📋 What do you need help with?
🔬 Research Context
Briefly describe your research question and study design:
Study type:
📊 Your Data
Data source:
Data characteristics:
- Number of samples: 86
- Number of groups/conditions: 4
- Sample size per group: 33/9/33/11
- Type of microbiome data: [e.g., 16S, shotgun metagenomics] 16S
💻 What you've tried
Current approach:
# Please share your current code (even if it's not working)
library(ggpicrust2)
# Your code here...
Current results or issues:
Describe what you're getting vs. what you expected.
🎯 Specific Questions
📚 Background Research
- [x ] I've read the package documentation
- [x ] I've reviewed the vignettes
- [x ] I've searched existing issues
- [x ] I've consulted relevant literature
Helpful resources you've already checked:
🖥️ Environment (if relevant)
- Operating System:
- R Version:
- ggpicrust2 Version:
🔄 Analysis Goals
What do you ultimately want to achieve?
⏰ Timeline
📖 Additional Context
Any other information that might be helpful for understanding your question or providing better guidance.
❓ Question / Help Request
Dear Caffery,
I hope you are well and thank you for the very helpful tool (:
I normally use the output of picrust2 (e.g. pred_metagenome_unstrat.tsv) as the input for ggpicrust2. But I was wondering if we should transform/normalize/standardize the picrust2 output before using it in the ggpicrust2 function. For example, should we transform/normalize it based on the tool we use (Aldex2 vs Deseq2 vs Maaslin2 vs LinDA.
Do you have any recommendations?
Thank you (:
All the best,
Carmen
📋 What do you need help with?
🔬 Research Context
Briefly describe your research question and study design:
Study type:
📊 Your Data
Data source:
pred_metagenome_unstrat.tsv)Data characteristics:
💻 What you've tried
Current approach:
Current results or issues:
Describe what you're getting vs. what you expected.
🎯 Specific Questions
📚 Background Research
Helpful resources you've already checked:
🖥️ Environment (if relevant)
🔄 Analysis Goals
What do you ultimately want to achieve?
⏰ Timeline
📖 Additional Context
Any other information that might be helpful for understanding your question or providing better guidance.