Skip to content

Are you missing .ctab files issue #186

Description

@zoe-elizz

Hello, I'm having an issue getting my .ctab files into R. My .ctab files were generated from Stringtie by specifying a ballgown readable output (-B). I have a similar directory structure to the example provided where I have set my working directory to my "RNA_seq2021" folder in R and in here I have my root folder "Bees", within which I have subfolders for each of my samples (VB_B1, VB_B2, VB_V3,...VB_B29) and in these subfolders are my 5 .ctab files. Below is the code I have run:

VB_sample<c('VB_B1','VB_B2','VB_B3','VB_B4','VB_B5','VB_B6','VB_B7','VB_B8','VB_B9','VB_B10','VB_B11','VB_B12','VB_B13','VB_B15','VB_B16','VB_B17','VB_B18','VB_B20','VB_B21','VB_B22','VB_B23','VB_B24','VB_B25','VB_B26','VB_B27','VB_B28','VB_B29')
VB_dir<-paste0('~/Bees/', VB_sample)
bee_bg2<-ballgown(samples=VB_dir, meas='all')
But I keep getting this error:
Error in ballgown(samples = VB_dir, meas = "all") :
Something is wrong: are you missing .ctab files? Do extra files/folders (other than tablemaker output folders) match your samples/dataDir/samplePattern argument(s)?

I have also tried running:

bee_bg<-ballgown(dataDir='~/Bees', samplePattern='VB_B', meas='all')
but get this error:
Tue Apr 19 09:24:15 2022: Reading linking tables
Error in file(file, "rt") : invalid 'description' argument

Any suggestions on what is wrong?? This appears to be a common error that people get and I have combed through logged issues on bioconductor and git hub and haven't found a real explanation on how they fixed it or what is actually wrong.

Activity

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

      Milestone

      No milestone

      Relationships

      None yet

      Development

      No branches or pull requests

      Issue actions