Cytometry in R - Week # 10 - Downsampling and Concatenation

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Welcome to the tenth week of Cytometry in R! This is the official start of the "Cytometry Core" section, which means we are one-third of the way through the course! For previous course emails, see here.

This week, we will start building some of the toolsets that will be very much needed for the high-dimensional and unsupervised analysis weeks, with our goal of cobbling together our existing knowledge to down-sample our .fcs files to a desired number (or percentage) of cells for a given cell population. Once this has been accomplished, we will explore how to concatenate these down-sampled files together, before saving them to new .fcs files (while hopefully updating the metadata correctly so that commercial software can visualize everything correctly). You can find this week's course material here.

For those in Baltimore attending in-person:

• This week we will meet on Thursday at 4 PM EST (Bressler Research Building 7-035).

For those attending online, our YouTube live-streams will be on:

• No live-stream for the Tuesday 2200 EST (Wednesday 0200 GMT+0) slot this week 😞

• Wednesday 1530 EST (Wednesday 1930 GMT+0) (timeslot for the rest of the summer)

• Thursday 1000 EST (Thursday 1400 GMT+0).

All recordings will be available immediately afterwards on YouTube.

Best Wishes-
David