CPM normalization on pooled bootstrapped scRNA-seq data

[Jade-Jung]

Hello, I have a question about converting scRNA-seq count data into CPM.
In the document (https://sysbiochalmers.github.io/Human-GEM-guide/gem\_extraction\_sc/), there is an example converting the pooled NKCells scRNA-sea data into CPM, but not about pooled bootstrapped data.

Is it recommendable to convert pooled bootstrapped data into CPM then use it to extract context-specific model? or just use pooled bootstrapped data into ftINIT?
Thank you.

[haowang-bioinfo]

@johan-gson maybe you can help

[johan-gson]

Hi, yes, convert to CPM

[Jade-Jung]

Thank you so much!