diff --git a/README.md b/README.md
index 22b263585..e949142df 100644
--- a/README.md
+++ b/README.md
@@ -278,6 +278,7 @@ GuardDog's behavior can be customized using environment variables:
 | `GUARDDOG_VERIFY_EXHAUSTIVE_DEPENDENCIES` | Analyze all possible versions of dependencies (`true`/`false`) | `false` |
 | `GUARDDOG_TOP_PACKAGES_CACHE_LOCATION` | Location of the top packages cache directory | `guarddog/analyzer/metadata/resources` |
 | `GUARDDOG_YARA_EXT_EXCLUDE` | Comma-separated list of file extensions to exclude from YARA scanning | `ini,md,rst,txt,lock,json,yaml,yml,toml,xml,html,csv,sql,pdf,doc,docx,ppt,pptx,xls,xlsx,odt,changelog,readme,makefile,dockerfile,pkg-info,d.ts` |
+| `GUARDDOG_YARA_PATH_EXCLUDE_REGEX` | Regex pattern to exclude files from YARA scanning (matched against relative file path) | _empty_ (disabled) |
 
 #### Archive Extraction Security Limits
 
diff --git a/guarddog/analyzer/analyzer.py b/guarddog/analyzer/analyzer.py
index fe8259593..57947e8a9 100644
--- a/guarddog/analyzer/analyzer.py
+++ b/guarddog/analyzer/analyzer.py
@@ -1,5 +1,6 @@
 import logging
 import os
+import re
 import yara  # type: ignore
 
 from collections import defaultdict
@@ -17,7 +18,7 @@
     validate_identifies,
     validate_mitre_tactics,
 )
-from guarddog.utils.config import YARA_EXT_EXCLUDE
+from guarddog.utils.config import YARA_EXT_EXCLUDE, YARA_PATH_EXCLUDE_REGEX
 from guarddog.ecosystems import ECOSYSTEM, LANGUAGE
 
 SOURCECODE_RULES_PATH = os.path.join(os.path.dirname(__file__), "sourcecode")
@@ -378,6 +379,22 @@ def analyze_yara(self, path: str, rules: Optional[set] = None) -> dict:
             log.debug("No yara rules to run")
             return {"results": results, "errors": errors, "issues": issues}
 
+        regex_exclude = None
+        if YARA_PATH_EXCLUDE_REGEX:
+            try:
+                regex_exclude = re.compile(YARA_PATH_EXCLUDE_REGEX)
+            except re.error as e:
+                return {
+                    "results": results,
+                    "errors": {
+                        "rules-all": (
+                            "failed to run rule: invalid "
+                            f"GUARDDOG_YARA_PATH_EXCLUDE_REGEX: {str(e)}"
+                        )
+                    },
+                    "issues": issues,
+                }
+
         import time
 
         # Get rule metadata to access max_hits
@@ -425,6 +442,10 @@ def analyze_yara(self, path: str, rules: Optional[set] = None) -> dict:
                             scan_file_target_abspath, path
                         )
 
+                        # Skip files matching global exclusion regex
+                        if regex_exclude and regex_exclude.search(scan_file_target_relpath):
+                            continue
+
                         # Check path_include patterns if specified (takes precedence)
                         if path_include:
                             patterns = [p.strip() for p in path_include.split(",")]
diff --git a/guarddog/utils/config.py b/guarddog/utils/config.py
index 1824cf11b..9ada21f18 100644
--- a/guarddog/utils/config.py
+++ b/guarddog/utils/config.py
@@ -40,6 +40,15 @@
     "pptx,xls,xlsx,odt,changelog,readme,makefile,dockerfile,pkg-info,d.ts",
 ).split(",")
 
+"""
+This parameter specifies a regex pattern to exclude files from YARA scanning.
+The regex is matched against the file path relative to the scan root.
+- Default: empty (disabled)
+"""
+YARA_PATH_EXCLUDE_REGEX: str = os.environ.get(
+    "GUARDDOG_YARA_PATH_EXCLUDE_REGEX", ""
+)
+
 """
 This parameter specifies the maximum uncompressed size allowed for archive extraction
 - Default: 2 GB in bytes
diff --git a/tests/core/test_sourcecode_analyzer.py b/tests/core/test_sourcecode_analyzer.py
index 6d92ebf02..ed9435ecb 100644
--- a/tests/core/test_sourcecode_analyzer.py
+++ b/tests/core/test_sourcecode_analyzer.py
@@ -6,6 +6,7 @@
 
 from guarddog import ecosystems
 from guarddog.analyzer.analyzer import Analyzer
+import guarddog.analyzer.analyzer as analyzer_module
 from guarddog.ecosystems import LANGUAGE
 
 pypi_analyzer = Analyzer(ecosystem=ecosystems.ECOSYSTEM.PYPI)
@@ -69,6 +70,49 @@ def test_get_snippet_file_not_found():
     assert snippet == ""
 
 
+def test_analyze_yara_excludes_files_matching_regex():
+    analyzer = Analyzer(ecosystem=ecosystems.ECOSYSTEM.PYPI)
+    rule = next(iter(analyzer.yara_ruleset))
+
+    class FakeCompiledRules:
+        def __init__(self):
+            self.scanned_files = []
+
+        def match(self, file_path):
+            self.scanned_files.append(file_path)
+            return []
+
+    fake_compiled_rules = FakeCompiledRules()
+
+    with patch.object(analyzer_module, "YARA_EXT_EXCLUDE", []), patch.object(
+        analyzer_module, "YARA_PATH_EXCLUDE_REGEX", r"\.min\.js$"
+    ), patch.object(
+        analyzer_module.yara, "compile", return_value=fake_compiled_rules
+    ), patch.object(
+        analyzer_module.os,
+        "walk",
+        return_value=[("/tmp/pkg", [], ["keep.py", "bundle.min.js"])],
+    ):
+        analyzer.analyze_yara("/tmp/pkg", {rule})
+
+    assert "/tmp/pkg/keep.py" in fake_compiled_rules.scanned_files
+    assert "/tmp/pkg/bundle.min.js" not in fake_compiled_rules.scanned_files
+
+
+def test_analyze_yara_returns_error_for_invalid_exclude_regex():
+    analyzer = Analyzer(ecosystem=ecosystems.ECOSYSTEM.PYPI)
+    rule = next(iter(analyzer.yara_ruleset))
+
+    with patch.object(analyzer_module, "YARA_PATH_EXCLUDE_REGEX", "("), patch.object(
+        analyzer_module.yara, "compile"
+    ) as yara_compile:
+        result = analyzer.analyze_yara("/tmp/pkg", {rule})
+
+    assert "rules-all" in result["errors"]
+    assert "GUARDDOG_YARA_PATH_EXCLUDE_REGEX" in result["errors"]["rules-all"]
+    yara_compile.assert_not_called()
+
+
 # Comment filtering tests