Hello, i have issues with using this scirpt "downsample_normal_gatk.sh"
The issues i found :
the Singularity image checking threw errors so i changed it to this :
" # Local image names with correct .sif filenames
singularity_local_images=()
for image in "${singularity_images[@]}"; do
image_name=$(basename "$image" | tr ':' '_')
singularity_local_images+=("$image_name.sif")
done
for i in "${!singularity_images[@]}"; do
image_uri="${singularity_images[$i]}"
image_file="${singularity_local_images[$i]}"
if [ ! -f "$image_file" ]; then
echo "Pulling Singularity image: $image_file"
singularity pull docker://"$image_uri"
else
echo "Singularity image: $image_file already exists. Skipping pull."
fi
done
"
"-f "not duplicated" seems to be an invalid syntax. https://github.com/biod/sambamba/wiki/%5Bsambamba-view%5D-Filter-expression-syntax
this command produced empty compressed files :
" $bedtools bamtofastq -i $OUTPUT_FILE -fq $OUTPUT_FQ | gzip > "$OUTPUT_FQ.gz" "
so i changed it to this :
" $bedtools bamtofastq -i "$OUTPUT_FILE" -fq "$OUTPUT_FQ"
gzip -c "$OUTPUT_FQ" > "$OUTPUT_FQ.gz" "
the problem is that the resulting subsamples give this error during pon build " [E::aux_parse] Incomplete aux field samtools sort: truncated file. Aborting" and " [E::sam_parse1] SEQ and QUAL are of different length samtools sort: truncated file. Aborting". I hope you can help me figure out why. Thank you in advance.
Hello, i have issues with using this scirpt "downsample_normal_gatk.sh"
The issues i found :
the Singularity image checking threw errors so i changed it to this :
" # Local image names with correct .sif filenames
singularity_local_images=()
for image in "${singularity_images[@]}"; do
image_name=$(basename "$image" | tr ':' '_')
singularity_local_images+=("$image_name.sif")
done
for i in "${!singularity_images[@]}"; do
image_uri="${singularity_images[$i]}"
image_file="${singularity_local_images[$i]}"
if [ ! -f "$image_file" ]; then
echo "Pulling Singularity image: $image_file"
singularity pull docker://"$image_uri"
else
echo "Singularity image: $image_file already exists. Skipping pull."
fi
done
"
"-f "not duplicated" seems to be an invalid syntax. https://github.com/biod/sambamba/wiki/%5Bsambamba-view%5D-Filter-expression-syntax
this command produced empty compressed files :
" $bedtools bamtofastq -i $OUTPUT_FILE -fq $OUTPUT_FQ | gzip > "$OUTPUT_FQ.gz" "
so i changed it to this :
" $bedtools bamtofastq -i "$OUTPUT_FILE" -fq "$OUTPUT_FQ"
gzip -c "$OUTPUT_FQ" > "$OUTPUT_FQ.gz" "
the problem is that the resulting subsamples give this error during pon build " [E::aux_parse] Incomplete aux field samtools sort: truncated file. Aborting" and " [E::sam_parse1] SEQ and QUAL are of different length samtools sort: truncated file. Aborting". I hope you can help me figure out why. Thank you in advance.