lab-solutions.md

Title: Lab solutions Authors: Brant C. Faircloth Address1: Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA 90095, USA Contact: Brant C. Faircloth ContactEmail: brant.faircloth at gmail dot com

Inputs

Attempt to list the input chemicals of all solutions here.

1. Tris-HCl
2. Tris-Base
3. EDTA
4. NaCl
5. CTAB

CTAB Pre-wash Buffer (for 100 mL)####

1. Add 10.0 mL 1 M Tris-HCl to a 250 mL container
2. Add 10.0 mL 0.5 M EDTA
3. Add 20.0 mL 5 M NaCl
4. Bring up to 100.0 mL with dH2O
5. Mix gently by inverting
6. [optional] Autoclave

CTAB Buffer (for 50 mL)

1. Add 5.0 mL 1 M Tris-HCl to a 50 mL conical
2. Add 14.0 mL 5 M NaCl
3. Add 2.0 mL 0.5 M EDTA
4. Add 1.0 g CTAB
5. Bring up to 50 mL with dH2O
6. Mix gently
7. [optional] Autoclave

CTAB Buffer (for 25 mL)

1. Add 2.5 mL 1 M Tris-HCl to a 50 mL conical
2. Add 7.0 mL 5 M NaCl
3. Add 1.0 mL 0.5 M EDTA
4. Add 0.5 g CTAB
5. Bring up to 25 mL with dH2O
6. Mix gently
7. [optional] Autoclave

TLE Buffer (for 250 mL)

1. Add 2.5 mL 1 M Tris-Base to a 500 mL graduate cylinder
2. Add 100 uL 0.5 M EDTA
3. Bring up to 250 mL with dH2O
4. Transfer to a 1 L glass container
5. Mix gently
6. [optional] Autoclave